LOCUS       DAOQKN010000001        43180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_316_length_43180_cov_5.787896, whole genome shotgun sequence.
ACCESSION   DAOQKN010000001 DAOQKN010000000
VERSION     DAOQKN010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 43180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43180
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_316_length_43180_cov_5.787896"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="PLJ64_00005"
     CDS             <1..617
                     /locus_tag="PLJ64_00005"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HNC91905.1"
                     /translation="GGAGALFIRGGKVVFRHVGSEIESPLGAAPVGQTTQVVGVWDGV
                     NMILYVNGVEVASKEAPGQPSGSSTLYLGLANVGLASPWLRGSLDEVSVYSHALEAGQ
                     VDQHFRADPPPPAEDNPSSHDPDQPAMDDHVVDDPDQPVGDDPDQSSTDDPVGSVEAE
                     SGGSSPKSGVKSNSKARRSALKKCQRMSNRSKRNRCLRHVRNRF"
     gene            694..975
                     /locus_tag="PLJ64_00010"
     CDS             694..975
                     /locus_tag="PLJ64_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91906.1"
                     /translation="MKLYVCWGTFQVPGAREHPCKIAYDALVQADFEPEVIKAHSVGF
                     LPRAVQTGARKKVEDATGSKWVPALETDEGEWISGSREIVAWAEQHARV"
     gene            complement(979..1326)
                     /locus_tag="PLJ64_00015"
     CDS             complement(979..1326)
                     /locus_tag="PLJ64_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91907.1"
                     /translation="MSAVVLGLLFIFILIMVTRPALLVGVSADDLAHSLANTTSPSGG
                     SNCRKEADGNWTCNFAPPDGSWRRYEVSVDWLGCWDATLPGADTSTSGTSSSPYDKSG
                     CIELGDVIRIESD"
     gene            complement(1366..2928)
                     /locus_tag="PLJ64_00020"
     CDS             complement(1366..2928)
                     /locus_tag="PLJ64_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91908.1"
                     /translation="MTTSHRLRSWGPPLAFIAVISAVLLAIFPKGFPNYDTIYYLLWG
                     REIADGLSPDYGAPLAPTPHPLYDLLGALFSPLGDGSITVAVVLAYVSLGLLAWLVYR
                     LGSEWFDRWIGVLAAFLVMTSAPVLSNGLRAYIDIPYMVLCLAALLIETKKPKAGWPV
                     LVLLALAGLLRPEAWLFSGFYFLWLALDFHVSPAEGADTSAPSRRAGISLGRLRIQLR
                     ESGIDRNLILMACLTAAGPVIWVLFDLITTSNPLYSFTGTRETVETLERDTGPLDVII
                     YGPRRLGEVMQWPGMVGAFFGVILTLWAMPKRAAIGVISAFLALVAFALMGASGLAII
                     PRYTMLAAAILFIFTAAAVLGWRLLEPGHRLRRIWQVAAAITVALWLIWLPNQYDLLS
                     TVDTDLSDQALVERDLEALVDDGAFNPPSDLPGETSVEDCLPISVPNHRAVPRLAFWL
                     DIRPSDVVSIAAADEQPDSGFFLAPANEFTVENFILDPGEPGRAVTTPPPGFEEVARN
                     RSWVLYTSCLAG"
     gene            3012..4745
                     /gene="dnaX"
                     /locus_tag="PLJ64_00025"
     CDS             3012..4745
                     /gene="dnaX"
                     /locus_tag="PLJ64_00025"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNC91909.1"
                     /translation="MSESTSLYRRHRPHSFDQVVGQQHVVRTLSNAITQDKVHHAYLF
                     VGSRGTGKTSMAKILAASLNCENGGPTLTPCGTCESCRSIAAGNSVDVIEMDAASNRS
                     VDDIRELRDRVAFAPTAGRWKVYILDEAHMLTKEAWNAFLKTLEEPPPNTVFILATTE
                     AHKVMPTIADRCQRFDFQRPSIEQLSEVLTRVAAAEQITIGAPAISMISRSASGSFRD
                     ALGTLDQLVSYSGNEIALDDVLEVLGAADADLLFGVVDKVIEADPAGVLTAVEQMARS
                     GRDPARFARDLLGHLRVLLVTQTTGQIPEAFAVTAADPDRLVRQAKAIGPANLIRTID
                     ELSAALGLIRDGDEARLAVEVALLKAARPDFDPSSEGLARRLERLESGVRPVAPPPIQ
                     ESPPAPSAAPTPPASEAAASSAPPSPPAVAEAEAAAPAVAPEPPKPQAAPTPPEPSAA
                     PTPAPEPEPYDLPKLEQVWPAVLDSLGASASGMVASYFDGTVPVALDGTKLSIGFPAD
                     AAFSRRNAERPECRQQLAEAIKVVTGDQFQVEYASLDSGQEVADPESEVMEEEDFIAR
                     VKSEFNAEEVI"
     gene            4745..5092
                     /locus_tag="PLJ64_00030"
     CDS             4745..5092
                     /locus_tag="PLJ64_00030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNC91910.1"
                     /translation="MARKGMGGMPGGMDMNKLLQQAQQMQAEMMQAQESLKDEVVEAS
                     AGGGMVKVKISGDLVVQDVVIDPDAVDPEDSEMLAEMVQAAINEAIRSAQQLAESKMP
                     QMPNMPGMPGLGG"
     gene            5089..5685
                     /gene="recR"
                     /locus_tag="PLJ64_00035"
     CDS             5089..5685
                     /gene="recR"
                     /locus_tag="PLJ64_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNC91911.1"
                     /translation="MSDGPAPLARLTAELSQLPGIGRRTAQRLAFHILRSDESDARSL
                     AQAIVEVKEKVGLCEICFNLAEGPRCRICEDHSRDESLICVVEEPADVIPIERTHEYR
                     GRYHVLGGALSPIDGIDAEDLRIAELRRRVEAGGISEVILATNPTTTGEATASHIADL
                     LRTQTKVTRLAAGLPVGADLEHADEVTLGRAMAGRGSI"
     gene            complement(5694..6743)
                     /locus_tag="PLJ64_00040"
     CDS             complement(5694..6743)
                     /locus_tag="PLJ64_00040"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNC91912.1"
                     /translation="MSEHIWYTHAPSPTAFGIALRKGWIHEEMARCGVEVRALAASSD
                     PDVQRLRRERTSPRAFRQGGNVAPLIARSRGNDVRLIGLSRTSSIHPVLALPESGYRT
                     AADLLGRRIAIPVRKNAVVDIPRATALRTVSNLLASAGLTLNDVELVAIPVEGSYFEA
                     RAKPGEPIADAPREGVAFQVSSAHSNELLALIRGEVDAIASEGSTAAIIASTFGLTTL
                     EPGRAVNRDLNNRSLLAVTVSGGLLDRGPGLVTSVLVQILAAAEWSIDEEAEARRLIA
                     LDAGIHEDLLDAAYSPRVSRQLGVDLRMDKVADLREQHDFLLDRGIIERPVDLDEFIE
                     PEPLKTALARRGVLV"
     gene            complement(6825..7637)
                     /locus_tag="PLJ64_00045"
     CDS             complement(6825..7637)
                     /locus_tag="PLJ64_00045"
                     /inference="COORDINATES: protein motif:HMM:NF025008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNC91913.1"
                     /translation="MPTGFEQDTAAEPLGEGRYAINLSDRWWVGRGPNGGYVAALMLR
                     AMTGEVASMEPEAALPPRSLSVHFLLRPEAGPAEIEVTVEHAGRVTAFLSARMVQSGE
                     VKAKAMAVFSGEREGPAFDNSLMPKVPPPEELEEWDTEQAPVAVFGRYRAMMIKGIPG
                     EAGERAETMGWIRLRDEQPVDPVLAAAVLDVWYPAPFVVFEKIPFAPTLEYTVHFPRT
                     IPEPGPPDWNLIRLTAEEGTEGHFTEDAELWSRDGKLLAKARQMALLRHPLG"
     gene            complement(7640..7996)
                     /locus_tag="PLJ64_00050"
     CDS             complement(7640..7996)
                     /locus_tag="PLJ64_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HNC91914.1"
                     /translation="MTDGSLRCPECGSEHTYELGGFSVCSLCAHEWDPAHDRTEIAGD
                     SAPEIRDSVGNVLTDGDTVTVIKNLKVKGASGPIKAGTKVKGIRLVNGVGDHDIDCRI
                     DGFGQMQLKSSVVKKA"
     gene            8062..9345
                     /locus_tag="PLJ64_00055"
     CDS             8062..9345
                     /locus_tag="PLJ64_00055"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNC91915.1"
                     /translation="MPERDDIVVMKFGGTSVAGPEEIKRAARRMVAAREAGNSVVAVL
                     SARGKTTDELVASAYEISDRPDPREMDMLLSTGERISCALAAMAINDMGHKAVSLTGS
                     QAGIVTDSSHTKAKIIDVKADRIREGLGEDKIVLVAGFQGVSTDSRDVTTLGRGGSDT
                     TAVALAAALGASVCEIYTDVTGVFSADPRIVPDARKLPVVSFEEMLDMAASGAGVLQL
                     RSVEYARNYNIPIHCRSSFEDLPGTLVLPESETMERPIITAVTHSTDEARVAVIGVKD
                     EPGIAGRIFGALADANVNVDVIIQNEPVHEGALADLSFTVETGDLATATDAIASLGDI
                     SQEVRTDEQIGKVSIVGAGMRSHPGVAAKVFEVLGEKEINIEMISTSSIRISCVIQAD
                     QVPEAVRSLHAAFDLGEEQILAEAPTGEHRPVTPS"
     gene            9348..10376
                     /locus_tag="PLJ64_00060"
     CDS             9348..10376
                     /locus_tag="PLJ64_00060"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNC91916.1"
                     /translation="MDESRYRVGVLGATGAVGSTILEILHERSFPASEVVAFASERSA
                     GKKVPFGEGELTCVVPDDESIQGIDILISSAGGSVSAEWAPKFSEAGAVVVDNTSYWR
                     MHEDVPLVVAEVNPEAARDHKGIIANPNCTTMVMMLVLAPIHREARLERLILSSYQAV
                     SGTGQKAIAELTEQAGQVLAGETVSYPAVYPHQIAFNVIPQVETFKDGDDYTTEERKV
                     MAETRKILGLGDEVKISATCARVPVFTGHSESLNIETREDLSPDRAREILGAAENVIV
                     VDNPGAGKYPTALDAAGRDETLVGRIRRDPGQEKTLNLWLAGDNLRKGAATNAVQVAE
                     LLVKNDWL"
     gene            complement(10373..11212)
                     /locus_tag="PLJ64_00065"
     CDS             complement(10373..11212)
                     /locus_tag="PLJ64_00065"
                     /inference="COORDINATES: protein motif:HMM:NF016873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HNC91917.1"
                     /translation="MPWNAEKPADQMDGNWLITEGDPESAVILHVPHASRAFPTRELN
                     RLVVDREGLDAELDAITDADTERLAAEAAGLAELRPWIFENRYSRLLVDPERFPDDRE
                     EMLEAGMGPVYLKTTTGAEFRRADEADDERLMSEYYRPYASAFERLVEQRLERLGRVL
                     LIDVHSFPRDPLPYELHSGEARPALCIGTDKFHTPQWLVDAISGLWPDTVELDQPFRG
                     CYVPLSFLESESRIAAVMLELRRDVVARWAGPDSPGRRGEPGLPGFVAAVADLFSRYD
                     PTG"
     gene            complement(11253..11729)
                     /locus_tag="PLJ64_00070"
     CDS             complement(11253..11729)
                     /locus_tag="PLJ64_00070"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNC91918.1"
                     /translation="MADHIVRSVIREVKPLKTSDPVGYAARLVIESGLPALPVVDEHG
                     KYAGLFGEREFMTAVFPGYVGTLMSARMIRRSLDETIERRIGSQEEPVRGYLTTDPVL
                     VEDDYADTQLAETFLHHRVMVVPIATEGRVHAVVPRSEFFQALYERIADIAEDFGD"
     gene            complement(11765..13018)
                     /locus_tag="PLJ64_00075"
     CDS             complement(11765..13018)
                     /locus_tag="PLJ64_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HNC91919.1"
                     /translation="MALAVATFLIVLALIASERVHRTKVALLGAAIVVLFVGQYNEEL
                     AIESVEFATLGLLAGMMILVYLTQQSGVYDYIAVKAGQLSRGRPFLLVVMLVTTVAVM
                     SGFLDNLTTILLIVPITFLLADTLDINPMPLILIEVMAANIGGAATLIGDPPNIMIGN
                     SANLSFNDFLINNMPGVVVILATVTVGLYFAYRSQLQVAEENRKYVQELDAAASIRDH
                     DELRRTLPVLIGTIILFFLHQYIHVEPATIALTGAAVGLMVTKTPIEEALEKIEWTTL
                     FFFIGLFVMVGALEVTGAIEKLAEGITSVTNGNRDAELVGIIWTSSLVGGIVDNIPLT
                     AAMIPVVEDIKAGSTDHAYWWALSLGACFGGNLTLISAACNVAASGMAEKAGRPIGFW
                     TFLKVGFPVTVASTVMATAYVLLRF"
     gene            complement(13066..14214)
                     /locus_tag="PLJ64_00080"
     CDS             complement(13066..14214)
                     /locus_tag="PLJ64_00080"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNC91920.1"
                     /translation="MFGGANSITLFRVAGIRIAVDWSWFIFLFIVIVWLSGFYGDILD
                     EPGSTQSYGLALASAIGFFGSILLHELGHAFEAKRSGIGIRTIRLWLFGGVAEMDQES
                     ESPGTEFRVAIAGPVVTLLIAVILCVIGIQAAGFDNFRDALEMRITAETNGPITMVAW
                     LAAVNILVLGFNLLPAFPMDGGRIVRSIAWKITGKRSAATRFAAGLGRIFGFIFIGLG
                     LLWIFNGNLFSGLWLAMIGFLVNSSARAASRSTVGDKLEGITVSQVMDTEPVSIPAEI
                     DCARALEEYFLRFGWSWFPVVDRHGSFLGMAVRDRLEAVPEVERDATPVISLVEINPG
                     FSVRSDAGLDRLLGNPQLRRFGALMVTDDQGKLAGVVTVEQLGRALQA"
     gene            14276..15502
                     /locus_tag="PLJ64_00085"
     CDS             14276..15502
                     /locus_tag="PLJ64_00085"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNC91921.1"
                     /translation="MANGTPMKLAAPHGEADQNWLRARLEELERIDRPSASAGERAAA
                     EWLVSRFSELGAEARIETEKAHGTYWWPIGIGTALGAAGGIAATRGRRLAGALLGAAG
                     AAAIATDFPPRPRYLRRFLPKRDTFNVVCELGDPEAERTVVFVSHHDAANAGLVFHPG
                     IPDLVSKTGAFQHLDTSPMLMAPVIGGPLLSAIGGLTGSRKLAAAGLLLSAGSTASMV
                     DIGLRKVVPGANDNGTAVIALLELARRFLADPPEGLRLILLSAGSEESFSEGMKAFGE
                     RHFPNLPVDSTFFINMDTIGSPYLTVLRGEGFLKMFEYPPEALILADRAAEEEGIELM
                     PNLRIHNGTDGLEALAAGYPVVSICSCTEHKQPANYHWPNDVSENVDFDTVESGIRLA
                     EAMARRLSRDWVSLPG"
     gene            15511..16755
                     /locus_tag="PLJ64_00090"
     CDS             15511..16755
                     /locus_tag="PLJ64_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC91922.1"
                     /translation="MAPALTLPLRNSVYRRLFSAQIVGLLGTGISTVALGLLAFSMGG
                     GNAGQILGVVLALKVATYVLVSPAIAAFAAGVSRKKLLVTMDVVRALAIGSIAFVGEI
                     WQVYVLIVIVSAASAGFTPAFQALIPDIFTDVDTYTEALSLSRLAYELEALVSPLIAG
                     LLLAVVGYTELFTLNSLAFLTSAGLVLTTAVPAVRDDALAARKLSQVSQGIRQFLRTP
                     RLQGLFLLDWAAAAAGAMVLINTVIYVKGQLGFGDSDVAWALGAFGAGSIVIALLLPT
                     LLRRFTDRSLMLLGGLILAASMFLFVAVGSLTTLMACWALAGIGTSAVLTPSGRLIQR
                     SGDSAGRPALFAAQFSLSHFCWLFTYLIAGFAGEALGLSEVALILGAIAAGASVLAAM
                     VWRPEPPARQASSTGSPQRLSR"
     gene            complement(16713..17048)
                     /locus_tag="PLJ64_00095"
     CDS             complement(16713..17048)
                     /locus_tag="PLJ64_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91923.1"
                     /translation="MADHGLSNHHSSTLAEIFDHQKHSIEWRKVESLLNAVGTVTRKH
                     NGHLKVEVGPETETFEVPHGKEINRQQLVDIRRMLSEAGYGPDGKAVEDQRDRDYRDN
                     RWGDPVDEA"
     gene            complement(17130..18803)
                     /locus_tag="PLJ64_00100"
     CDS             complement(17130..18803)
                     /locus_tag="PLJ64_00100"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HNC91924.1"
                     /translation="MLETAEAPQHETHTVFNQSTPLENVNLYEIDLPLQEAVNREGAG
                     WAEDRIKALGEICGQPEVLLAGRDANEFPPRLKAFDRFGNRIDEVEFHPAWHELMALG
                     IEHDLHALPWREPGIGANVARAALFTQLTQVEAGVGCPISMTFSVIPSLRNQPEVAEE
                     WEPRFTSLEYDGKLKPADQKPGCLAGMAMTEKQGGSDVRANTTRAVPLNGGGPGGEYE
                     ITGHKWFCSAPMCDAFLVLAQTDAGVSCFLLPRFTPNGERNRFHIQRLKEKLGNKSNA
                     SSEIEFRGAWARMIGEDGRGIRTIIEMVSHTRLDCAIGSSAGMRTGVAQATHHAMNRS
                     AFGKKLIDQPLMENVLADLCVESEAATATVMRLARAYDEAAAGDEGAALLQRLATPVL
                     KYWICKRAPWHAVESLECFGGNGYAEESGMPRLFRESPLMSIWEGSGNVQCLDALRAM
                     VKSPASYEAFFSEVGEAAGADSRLDSFVEKLQQEITGEPETLEIRARRVVEGMGLAFQ
                     GSMLVRHGDPAVADAFCASRLAGDWGEAFGTLPAGTDFKTIIQRSAPPV"
     gene            18934..20148
                     /locus_tag="PLJ64_00105"
     CDS             18934..20148
                     /locus_tag="PLJ64_00105"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HNC91925.1"
                     /translation="MSKIKVTNPIVELDGDEMTRIIWSFIKEQLILPYLDVDLKYYDL
                     GIESRDDTDDQITVDAANAIKEHGVGVKCATITPDEARVEEFDLKEMYRSPNGTIRNI
                     LGGVIFREPIVISNVPRLVPGWTKPIIIGRHAFGDQYRATDMVVEGEGTLTMTFTPKD
                     EGEPIEMHVHDFPGGGVAMAMYNHDDSIRDFARASFRYGLERGYPVYMSTKNTIMKRY
                     DGRFMILFQEVFDDEFKDEFDQAGLTYEHRLIDDMVASALKWEGGYVWACKNYDGDVQ
                     SDTVAQGFGSLGLMTSVLMTPDGKTVEAEAAHGTVTRHYREHQKGNATSTNPIASIFA
                     WTRGIAARGRMDETPEVSQFGETLEKVCIETVESGKMTKDLALLVGPDQEWQTTQEFL
                     ASIDENLQKAMA"
     gene            20179..21165
                     /locus_tag="PLJ64_00110"
     CDS             20179..21165
                     /locus_tag="PLJ64_00110"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNC91926.1"
                     /translation="MSSTAKVTVTGAAGQIGYALLFRIASGQMLGPDTKVHLSLLEIP
                     AAVKAAEGTAMELDDCAFPLLEKISISDDPKEAFDGVNVGLLVGARPRGPGMERADLL
                     EANGGIFKPQGEAINAGAADDVKILVVGNPANTNALIAASAAPDVPKERFTAMMRLDH
                     NRAIAQLANKLGRSVSDVKRMTVWGNHSATQYPDIGNALVDGAPAPAQVDDEVWVREE
                     FIPRVAKRGAEVIDARGASSAASAANAAIDHVRDWVLGTPSEDWVSMGIPSDGSYGVP
                     EGIIAGFPVTCSGGEYSIVEGLEIDEFSREKIDANAAELMEERNAVADLGLL"
     gene            21234..22007
                     /locus_tag="PLJ64_00115"
     CDS             21234..22007
                     /locus_tag="PLJ64_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91927.1"
                     /translation="MRGKFRKPTYAEIVATLALFIALGGISYAAIKIPNNSVGTKQLK
                     KSAVNSAKVRDRSLLAKDFRKGQIPRGATGAQGAPGMIGPTGAAGATGASGIPGAVGA
                     TGPTGAAGVTGENGDPGQPGGAGPTGPTGVTGPSTTAVSSGVVVNLGLGPDYFPISGA
                     SSPQGAPDTVASLSPGVPVTASNLDVKLTAAPFAGFPRIVSLLDDGIAVLSCVIASGT
                     TCHADTSALIDPGSELVMVSSVLAFPSITTARFGFTLGP"
     gene            22058..22858
                     /locus_tag="PLJ64_00120"
     CDS             22058..22858
                     /locus_tag="PLJ64_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HNC91928.1"
                     /translation="MGNRGKGLKGFKSKLRRPNFAETIAVIALFVALGGASYAAIKIP
                     KNSVGTKQLKKNAVTSAKVKNRSLLAADFKAGQLPAGATGATGLVGPRGATGEQGIPG
                     FPGTPGTPGINGIDGLPGSPGPAGATGATGATGSTGATGTGGFSAMTGRTSLGSSTQF
                     FAVSGSSVAGATATGLGMITPAVPVTLKNLMVVTDTAPGTGITRTFVIQEDGINVISC
                     TLGGIATACFNTSGGGVINAGSTLTMFDTVTGGSAAPSEARWGFTIGS"
     gene            22885..23850
                     /locus_tag="PLJ64_00125"
     CDS             22885..23850
                     /locus_tag="PLJ64_00125"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HNC91929.1"
                     /translation="MSERGKVVILRELLPAGRQLLEEKFQVTSLGLDADRSQMKAAAA
                     GADGLVTDLTFAVDDELLGAAGPQLKVVANFGVGYDNVDLAACAARGVTVTNTPGALT
                     NATAELALALAMAAARDLPAAEKDLREGAWQGWDPRRWRGFELSGASIGVVGAGRIGG
                     RFMELVAGFGGERLYTSRQPKPDLENRLGAERVELDELLARSDLISLHLPGSRESRHL
                     IDERALRLMKRTAVLVNTGRGTLVDSAALIRALKEDRIGAAGLDVYEGEPDFDPGFLT
                     APRVALAPHIGSATYRSRDEMSRLVAANVIAVLEGREPPNPVPGV"
     gene            complement(23899..24537)
                     /locus_tag="PLJ64_00130"
     CDS             complement(23899..24537)
                     /locus_tag="PLJ64_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNC91930.1"
                     /translation="MGSSERTERLAASPDLFENRLLNFFSRVHPVVPLLIYGPIIALL
                     VWLGVDRGLSALATVGLFVGGILIWTLSEYWLHRLLFHWTPKFRGGDKINFIIHGVHH
                     DHPNDKMRLVMPPAASIPLALIFYAAFMLIFGSPYGFVLFAGFLTGYLGYDYTHYYLH
                     HFVPKSSIGKKVREHHMRHHFQDHHYGYGVSTPFWDHVFRTVPRSRKADRGS"
     gene            24595..26064
                     /gene="ahcY"
                     /locus_tag="PLJ64_00135"
     CDS             24595..26064
                     /gene="ahcY"
                     /locus_tag="PLJ64_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNC91931.1"
                     /translation="MSETATATQDFKVADLSLADYGRKEIRLAEHEMPGLMATRREFA
                     DSKPLAGARITGSLHMTIQTAVLIETLVELGADVRWASCNIFSTQDHAAAAIAVGPNG
                     TPEDPQGVPVYAWKGETLEEYWWCTEQALTWPGTGADGSVGPNMILDDGGDATLLIHK
                     GVEFEKAGAVPDPAGADSEEFRIVLQTLTDSLAADSSKWTEIAKGIKGVTEETTTGVH
                     RLYEMLENGELLFPAINVNDSVTKSKFDNLYGCRHSLIDGINRATDVMIAGKAAVVCG
                     FGDVGKGCAESLRGQGARVIITEIDPICALQASMQGYEIRRLDDVVDSADIFITTTGN
                     KDIITADHMAKMKHQAIVGNIGHFDNEIDMVGLEATPGIEKIEIKPQVHEYRFADGHS
                     IIVLSEGRLLNLGNATGHPSFVMSNSFTNQVIAQIELYAKADDYELGVFVLPKHLDEK
                     VARLHLQAVGADLTELTEEQAAYIGVKVEGPYKPDHYRY"
     gene            complement(26370..28703)
                     /locus_tag="PLJ64_00140"
     CDS             complement(26370..28703)
                     /locus_tag="PLJ64_00140"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNC91932.1"
                     /translation="MRFRFSILVMALVAMAFSAAEASASPWIHAHRGGPLENGKAAQA
                     ENSSSGFAASIERGFVLEADVKLTSDKVPVIIHDDTLDRTTTCTGPVSAKTLDEIESE
                     CALDVKGIGDAATNLRAGYPGPTPRIPTLAAFLQVLKRTGAKANIEIKNLPTDNDWDP
                     TFEFAEIVANTVKNSGVPSSQLIIQSFLIDNLTTFKSIDPDPQTSYLTINLVNPIAIS
                     AARDNGIDWVSPEWPIDQAFVSDAHHAGLQVVPWTVDDAAGVREATRLGVDALITNDP
                     LMARTQVRKVAPPLAAIPKAPNLKSCNATFARDTRRPALAMLGRKDARGGPRVFAMQF
                     KQEARHIKTYASFRKKIECMIRKWVVPYKAKGRPNLVAFNEDVGLMTLGTGSRGAAAR
                     AAFAKPSSVTSCTGEAPPCRAIYSLMQVTAAYAGPDGEYTSRYSIPNPFTRGFVAAAD
                     TDARGWMQVFSDMARRYGIYIVGSNTQPRFRESQDPAEINLFRDPDLPKPKSVYVATG
                     PEVYNEAFMWGPKPVTQEGPRPLRNVVASNLKVPLTPIEQGLGVTAGPATGADAIANL
                     KPYRIPGTKARVGFATSLPAFRFGYDLGSPVSTDAPCADVSVTYMRCLSHLGTNLVMQ
                     DEANPGEWASPEGTYWQPLDWMGSTWRSVVDPGVKFTYNVTPHMVGNLGDLPFDGQTA
                     ITQRGLLGKKKCNYVGNRKLLAEDDPSYAGYAGPKRQFITLAPWVRKDGSRAELRKTG
                     AALLAASGKKMENRYLETAAIADLPFPPKKHRANCVS"
     gene            28946..29140
                     /locus_tag="PLJ64_00145"
     CDS             28946..29140
                     /locus_tag="PLJ64_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91933.1"
                     /translation="MSRSSKKVPSCEDCYFRQKMLCALDLEGPCGTFRESRPEGLVPP
                     RQPMLLLRPPRWADRQIGAS"
     gene            29240..30031
                     /locus_tag="PLJ64_00150"
     CDS             29240..30031
                     /locus_tag="PLJ64_00150"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNC91934.1"
                     /translation="MKLTVLGKSPAWQDADGACSGYLVTEGDTTLLLDCGNGVFGKLR
                     KFVDYVDVDAIVISHHHVDHIIDLIPYGFALKFAPRQQPVPVDRWPGTGNPARPKLFV
                     PPGATGFFNRVSDSWGTGNLLEDAFDTAEYDPEGRLEIGPISISFTEVPHYITTYAMD
                     LTGPDGSRITYGADCCPNPALVELASGTDLLIAESTLPRPERTGVRGHLTPAEAGEHA
                     KLAGAGELILTHISDELDPGWAVEQASSVYEGPVQVAYEGLQVEV"
     gene            30065..30532
                     /locus_tag="PLJ64_00155"
     CDS             30065..30532
                     /locus_tag="PLJ64_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNC91935.1"
                     /translation="MSTRDPFANFARMRREMDELLGGTWGRATYISRRSSGFSPNVDV
                     YYSGDPQRAVVKCDLAGIDLGSVGIEVSGRELTIVGERPVQETEGRVYQQVEIPTGPF
                     RYVVELQVDVDSERATATYDDGVLRVELPLKEEAEVTRRVPVDRSRKGRVIDG"
     gene            30525..32951
                     /gene="lon"
                     /locus_tag="PLJ64_00160"
     CDS             30525..32951
                     /gene="lon"
                     /locus_tag="PLJ64_00160"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNC91936.1"
                     /translation="MAEPGESVVPVLEVVSGSDEEVEEALRGEQELPEAMPVLPLRDT
                     VAYPDTLAPLGVGQERSIKLVDDVLAGNRMLVMVASRDPEEDTPSPDQLHGVGVVGVV
                     TRMLKVPDGTIRVLVQGSQRVRLGDFVTEKPYLVARITEMPDVIEETPELEALTRNVQ
                     KTFSDIIEQIPYLPEELQLAVTNVDDPSALSHLIAGALRITTDEKQLLLEEVDVAKRL
                     RMLSEILARELEVIQLGSQIQSEVQSSIDKGQREYFLREQLKAIQTELGEEDEQTAEI
                     NELRERIEAAKLPEHAHQAAERELSRLEKLPPAAAEHGVIRTYLEWLVDLPWSKQSKD
                     NLDIEHARGVLDADHYDMEKIKDRILEYLAVRKLNPDSPGPILCFVGPPGVGKTSLGQ
                     SIARALGREFERISVGGVRDEAEIRGHRRTYIGAMPGTIVRALRDAGTRNPVFMIDEI
                     DKMGSDVRGDPSSAMLEVLDPAQNDAFRDHYLDMPFDLSEVMFIATANILDTIPGPLR
                     DRMEVIQLAGYTADEKRHIARRYLVPRQIRANGLKPSQISFTDPALTAIIEEYTREAG
                     VRNLEREIGTVCRKVARTVAEKGGKAKKVKVSGKKVRELLGRRRVFSETRRRTTVPGV
                     STGLAWTPTGGDVLFIEATAMPGNGKLVITGQLGEVMRESAQAALSYVRANARDLAPG
                     LDEKWFAEHDLHIHVPAGAVPKDGPSAGVAITVALVSLITGRPVRNDVAMTGEVTLTG
                     QVLPIGGVKEKSLAAQRAGIKIVILPGRNEGDVEEIPEYERAEVEFFYADKVGDALAV
                     ALEGNLKGKQ"
     gene            33024..33485
                     /locus_tag="PLJ64_00165"
     CDS             33024..33485
                     /locus_tag="PLJ64_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91937.1"
                     /translation="MGTENISGKASRAYSSARENQHVRRLIEDEELRASLIAAAVAGR
                     KAFQRVQSNRSSAVESVTQDKKVKKELSSAAESLRDAADRLKAPPKKKRHPIRNLLLV
                     GVVTGGLVLVFSESARKSLLDAIFGAEEEFVYTSTTSTETAQTNGTPVGTE"
     gene            33917..35749
                     /gene="typA"
                     /locus_tag="PLJ64_00170"
     CDS             33917..35749
                     /gene="typA"
                     /locus_tag="PLJ64_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNC91938.1"
                     /translation="MARRDDMRNVAIVAHVDHGKTTLVDAMLWQSGAFRQGQDVNERV
                     MDSMDLEREKGITILAKNTAVQYGEIKLNIIDTPGHADFGGEVERGLTMVDGVVLLVD
                     ASEGPLPQTRFVLGKALEAGLPVILVVNKVDRPDARIGEVVDEVYELFMDLGADGDQI
                     EFPIVYTNGKKGWASLEEGLEGEDLKPLLDLMVEHIPAPEYEEGHPLQAWVTNLDASP
                     YLGRLALCRVMQGTIKAGSQVAWCRADGTVERAAVSELFVTEALERVSTDQAGPGEII
                     AVAGIPEVTIGETLSDPEDPRPLPVIKVDEPSLSIVVGINTSPLAGRDGDKVTARQIQ
                     NRLDQELLGNVAIRVLPTDRPDAWEVQGRGELQLVVLLEMMRREGFELTAGQPRVLTR
                     DVDGQMHEPMERLVIDVPEEHVGAVTQLLAARKGRMENMTAQSDSWTRLDYVIPARGL
                     VGFRTEFLTETRGTGILHSIFEGWEPWAGDITTRATGSLVADRQGSTASFALFGLQER
                     GAMFVGPGEDVYEGMIVGEHARANDLDVNAVKEKQLTNMRSSTSDVLVRLTPPKPLTL
                     ESAIEFLRSDECVEITPNSIRLRKVDLDQNDRVKAARKLKNLSR"
     gene            35821..37728
                     /locus_tag="PLJ64_00175"
     CDS             35821..37728
                     /locus_tag="PLJ64_00175"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNC91939.1"
                     /translation="MNSRPTWGQRLRYAFDNMMSRGTPALIFWLAAATLALIVVFTIV
                     FLVFGLSPEGGDPHKGVIGEAYFTLLHALDPGTITGDDGDWVFLFLMFLVTLGGLLIF
                     SALIGVLSTGLDARLQELRKGRSLVLEEGHTLILGWGKPVFTILAELDIASEGEADPV
                     VVVLSETDKVVMDDTLRERSDLKNLRVVTRHGSPIDVDDLALARPELASATIVLAPLE
                     EPEPDAYVIKVILALTQLLQGREVERGIVAEISDPANLSPARLAGAGQAVLLDGRETI
                     AKLLVQAARQSGISVAVQELLDFDGHEIYIDDASRFEGIDFGTLLGRLERCSVIGLLD
                     VDDMVLLNPPGSKVISSGDRLIAVAEDDTVLELFEESVSGPDFEQILAADERSPSPER
                     VVILGWNKGAQPVITHFDAFLAPGSRIEVVVDPGLGRAPEVDSHQRLANTDVNFRHGS
                     TTDRSTLEELELGQADHVMVLACSDDFDAQRADARTLVTLLHLREINDGLEPGFSIVS
                     EMVDERSRRLAEVAEVDDVIVSENVISLLFAQISQNHHLADVFTQLFSPEGSEIYLRP
                     AGTYVALNEEVSFATMVEAARRRGECAIGYRSASQSDDPDSGFGMTINPPKSRVFKVT
                     QGDSLVVLAEE"
     gene            complement(37945..39657)
                     /locus_tag="PLJ64_00180"
     CDS             complement(37945..39657)
                     /locus_tag="PLJ64_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNC91940.1"
                     /translation="MRLFPSIRPYRREWLRPDLMAALTVWAVLVPEALAYATIAGVSP
                     IVGLYAAPGALLIYAALGSSRKLVTGGDAATAALSAAIVGGIVAGDSSAFVQTTAALA
                     ICVGLIAVMAGVARLGFVVNFISIPVLRGFIIGLALTIIAGQIPHLFGVESSNGNFFD
                     RIWVFVTHLDQVDLPTVAIGLTSLAIILICKYRHPSAPGSLIAVGLGIAAVSLLDLGS
                     DGVAIVGEIKSGLPSVALPNVGLQDLGDLVAGAVGLMLVGFAESLAAAKAYPDEDEEL
                     DADRELIGTGAANVGAGLFGGFVVTGSFSKTSINSESGARSQMAGIVVALMVVITMLF
                     LTGLFEDLPEAALAAVVIAALIHAVDVVGLSELARIRDRGNRLNPANRPDFIAAVAAL
                     IGVTAFDILPGLFIGIVVSLVLLLYRSSRPHVTRLGELRAQPGHWGDLQRHANAVEVP
                     QIVVLRLEAAIYFANSDEIRQAVIRSLAADTRAVVLDVETVPYIDVTAAYMLKALREE
                     LDQRQVRLVIARDVGQVEDMMRSTGLEVLLTDSYVSIDQAVDSVTAGIRSTSGDRPSG
                     AATT"
     gene            complement(39654..40736)
                     /locus_tag="PLJ64_00185"
     CDS             complement(39654..40736)
                     /locus_tag="PLJ64_00185"
                     /inference="COORDINATES: protein motif:HMM:NF024905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HNC91941.1"
                     /translation="MRSLIEDRFSSLRFASLRIIQICLAAGVAWLIATRVVGHYEPFF
                     APISVVLVLGLAIEQRGRRAYEIAFGVALGIAIADLIVLALGTGTWQLMLVIGLAMSA
                     AVLSGGGVMLVNQAAISGVLVATLPAPDLADTTHRFVDALVGGGVALAANAITPVDPV
                     RLVRRYLEPLLARLSGTLVDIADALDHESHEEIVAALERARSLDPAVREMKIAVEAGQ
                     ETISLAPTRRRKRGRVDQLAAAAEPIDLMIRNTRVLARACQRAIDLDEHIPPVVADSI
                     RDLATSVSRLDRHLGGAPVRSTAREAALRAAAKATAALEETSNLSVSVIVGQIRSAAT
                     DLLLGLGMTNDQALDQVRSAREKLGI"
     gene            complement(40846..41709)
                     /locus_tag="PLJ64_00190"
     CDS             complement(40846..41709)
                     /locus_tag="PLJ64_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91942.1"
                     /translation="MRPGPVQSTAEDNLLYGAVHLNVTDLERSLVFWRDLIGMVELGD
                     GESGAVRLGVKGEPDEPGKELVVLHPGATRPVQRGYSALYHLAIHMPSEAEFARILAR
                     LFAARYPNAPTDHVTHWATYLDDPDGINVEIAFETIDRVGEIDIVGGRPLIIDSQGRR
                     HDMTEPLGLEEVFSHREDEDLNGPLPPGTRIGHIHLYVGDLDAARTFYEEIGFSPHMN
                     ADGIGFADLSLGGTFPHRIAVNVWQGIGAPQAPEGTAGMRCFELDDPRGGEVRELADP
                     AGNRLIIRPAA"
     gene            41754..43106
                     /locus_tag="PLJ64_00195"
     CDS             41754..43106
                     /locus_tag="PLJ64_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91943.1"
                     /translation="MRNRAPVLVLIGCLILSAALIIHAGRDLTFFFDEWQLLLASDPL
                     SLSTLLEPHNSQPSFMTSSIYGLLLETVGMSSQLPYRLVAVAASIACVTLLYLLVRRR
                     TDPWLALVMILPVLGLGVAWEALLLALSMNFLIGIATGLGALLALESRSRRGDLLACF
                     LLCCSLTCGGVGLAFLAGACVDVLVRRDARRAWIPAVPVLLYGLWSFAFGDEDSPASA
                     DNALGLPDYLYKTSAAGIRSVTGLAADLFPARPAFFLSVFIFALLLATVVWRLFFAKR
                     PYSDRVLVAGGALLAFWVLGGLALGEGRAPDASRYQYPSAILLILLVASLLDGLKLPR
                     WLPAALLPLALISLGLNVSQFSDGRDFLLKQTEITRAAIGQVEIEDPVPNFSLVEEAT
                     GSPFQRLIETDAYLRAEKRFGSPAYSPAQIRASGPAGREAAGGVAFAAALARKLAHDK
                     "
BASE COUNT         7734 a        14055 c        14051 g         7340 t
ORIGIN      
        1 atggcggcgc cggggcactg tttatccgag gcggcaaggt ggttttccgt cacgtaggaa
       61 gcgagatcga gtcgcctctc ggtgcggcac cagtcgggca aacgactcag gtggtcggag
      121 tctgggacgg cgtcaacatg atcctttacg tcaacggggt ggaagtcgcc tctaaggaag
      181 cccccggtca gccttccggc tcctcgactc tctatctggg attggccaac gtcgggctcg
      241 cctcaccgtg gctgcgcggc tcactcgacg aggtctctgt ttacagccat gcccttgaag
      301 ctggtcaggt cgaccagcac ttcagggcag atcctccacc cccagcggag gacaacccgt
      361 cgagccacga ccccgaccaa cctgccatgg atgaccacgt agtggacgac cccgaccaac
      421 cggtcggcga cgaccctgac cagtcgtcca cagatgaccc ggtcggtagc gtggaagccg
      481 agagcggcgg atcgtcaccg aagtccggcg tcaagtcgaa ctccaaggcc agaaggtccg
      541 cattgaagaa gtgccaacga atgagcaacc gctcgaagcg caaccgctgc ctgcgccacg
      601 ttcgtaaccg gttctagagc cacgctaaac cgcacttttt ccacaaatga tgtgggaaaa
      661 cgacagcttc acggcgccga ttagtctggt cccatgaaac tctacgtctg ctggggaact
      721 tttcaggtgc cgggagcgcg ggagcatccc tgcaagatcg cctatgacgc actggttcag
      781 gctgacttcg agccggaggt catcaaggcc cacagcgtcg gtttcctgcc ccgcgccgtt
      841 cagaccggcg cccggaaaaa ggtagaggac gccacgggaa gcaagtgggt gccggcgttg
      901 gaaacagacg agggagaatg gatctcggga tccagagaga tagtcgcctg ggccgagcag
      961 cacgcccggg tctgaccttc agtcgctctc gatcctgatc acgtcgccca gttcgatgca
     1021 gccggacttg tcgtaagggg agctggaggt tccggaggtg cttgtgtcgg cgcccggcaa
     1081 ggtggcgtcc cagcagccca gccagtcgac gctcacctcg taacgtctcc aggatccatc
     1141 cggaggggcg aagttgcagg tccagtttcc atcagcttcc ttgcggcaat tactgccgcc
     1201 gctcggcgag gtggtgttgg ccagcgagtg ggccaggtca tcagctgaaa cgccgaccag
     1261 aagtgcaggc cgagtgacca tgatcagaat gaagatgaac aggagcccca ggacgacggc
     1321 gctcaaggcc ccgaggatct ttacgccctt ccaccacacg gtggctcagc ccgccaggca
     1381 gctggtgtag agcacccagg accggttgcg ggcgacctcc tcgaaaccgg ggggaggcgt
     1441 ggtgacggca cggcccggct cgcccgggtc gaggatgaag ttttcgaccg tgaactcgtt
     1501 ggccggggcg aggaagaagc cggaatctgg ttgttcgtcc gcggcggcga tcgacacgac
     1561 atcagatggc cgaatgtcca gccagaaggc gagacggggc acggcccgat ggttcgggac
     1621 cgagatcggc aggcagtcct caaccgaggt ctccccaggg aggtcgctcg ggggattgaa
     1681 ggcaccgtca tcgacaaggg cctcgaggtc cctttcgacc agggcctgat cgctcaggtc
     1741 ggtgtcgacg gtgctgagca ggtcgtactg gttgggcagc cagatcagcc agagcgcgac
     1801 cgtgattgcg gctgccacct gccagattcg ccgcagcctg tgaccgggct cgagcagccg
     1861 ccagcctagt accgcggcag ccgtgaatat gaacaggatc gccgcggcca gcatcgtgta
     1921 gcgggggatg atcgccaggc ccgaagcacc catcagggcg aaggcgacca gggccaggaa
     1981 agccgagatc accccgatag cggcccgctt cggcatcgcc caaagggtca ggattactcc
     2041 gaagaaggca cctaccatgc ccggccactg catcacttcg ccaagccggc gtggtccgta
     2101 gatgatcacg tcgagtggtc cggtgtctcg ctccagcgtc tcgaccgtct cgcgggtgcc
     2161 ggtgaaggag tagagggggt tcgaggttgt gatcaggtcg aagagaaccc agatcaccgg
     2221 tccggccgcc gtcaggcagg ccatcaggat caggttcctg tcaatccctg actccctcag
     2281 ctggattctc aggcgtccga ggctgatccc ggcgcgtcga gacggagctg aggtgtcggc
     2341 gccctccgca ggcgacacgt ggaaatcgag ggcgagccag aggaagtaga agccggagaa
     2401 gagccaggcc tccggtctga gcaggccggc cagggcaagc aacacgagta cgggccagcc
     2461 agccttcggc ttcttcgttt cgatcagcag ggcggcaagg cagagcacca tgtacgggat
     2521 gtcgatgtat gcccggagac cattgctcag gaccggtgcg ctcgtcatca ccagaaacgc
     2581 ggcgaggacc ccgatccagc gatcgaacca ttccgagccc agccggtaga ccagccaggc
     2641 cagcaggccg aggcttacgt aggcgaggac gaccgcgacg gtgatcgagc cgtcgcccag
     2701 cggtgagaaa agcgccccga gaaggtcgta gagcgggtgc ggggttgggg cgagcggggc
     2761 gccgtaatcc gggctgaggc catcggcgat ctcccggccc cagagcaggt aatagatcgt
     2821 gtcgtagttc gggaagccct tgggaaagat cgccagcagg accgccgaga tgacggcgat
     2881 gaaggcaagc ggcggccccc aggaccggag tcggtgagac gtcgtcatcg ggccgtaaag
     2941 cctgtcacat gaggcgggta cactgggctg gtgccgcccc gggagactgt tccgttgcgg
     3001 cggaccagct catgagcgaa tcgacctccc tctaccggcg acaccggccc cactccttcg
     3061 accaggtggt cggacagcaa catgtggtcc ggaccctgtc caatgcgatc acccaggaca
     3121 aggtccatca cgcctacctg ttcgtcggct cacggggtac cggcaagaca tcgatggcga
     3181 agatcctcgc cgcctcgctg aactgcgaga acggcggtcc gaccctcacc ccctgcggca
     3241 cctgcgaatc ctgccgctcg atcgcggccg gcaattcggt cgatgtgatc gagatggatg
     3301 cagcctccaa ccgctcggtc gacgacatcc gcgaactgcg ggaccgggtc gccttcgccc
     3361 cgaccgccgg ccgctggaag gtctacatcc tcgacgaggc gcacatgctg accaaggagg
     3421 cctggaacgc cttcctcaag actctcgagg aaccgccgcc gaacacggtc ttcatcctcg
     3481 ccacgaccga ggcccacaag gtgatgccga ccatcgccga ccgttgccag cgcttcgatt
     3541 tccagcgacc ctcgatcgaa cagctctccg aggtgttgac ccgcgtcgcg gccgccgagc
     3601 agatcacgat cggcgctccg gcgatctcaa tgatcagccg ctccgcttcg ggaagtttcc
     3661 gcgacgcgct cggcactctg gaccagctcg tctcctacag cgggaacgag atcgcgctcg
     3721 acgacgtgct cgaggtgctg ggggcggccg acgccgacct gctcttcggt gtggttgaca
     3781 aggtgatcga ggccgacccg gccggcgtcc tgaccgcggt cgagcagatg gcccggtccg
     3841 gtcgcgaccc ggcccgcttt gcccgcgacc tcctgggcca ccttcgggtg cttctggtca
     3901 cccagacaac cggccagatc cccgaggcat tcgcggtgac tgccgccgac ccggaccgtc
     3961 tggtccgcca ggcgaaagcg atcggaccag ccaacctgat tcgcacgatc gacgagctgt
     4021 ctgccgccct gggcctgatc cgtgacgggg acgaggcgag actcgcggtg gaagtggcgc
     4081 tgctgaaagc tgcccgtccc gacttcgatc cgtcaagtga aggattggcg cgacggctgg
     4141 aaaggctcga gtcgggagtc cgccccgtgg ccccgcctcc gatccaggaa tcgccgccgg
     4201 cgccgtccgc cgccccgact ccgccggcgt ctgaggcagc tgcgtcgtca gcgccaccat
     4261 cgccgcccgc tgtcgcagag gcggaggcag ccgctccggc cgtcgcgccc gaaccaccca
     4321 aaccccaagc tgcaccaaca ccacccgaac catcagctgc gccgacaccg gccccagaac
     4381 cggagcctta cgacctgccg aagttggagc aagtctggcc ggccgtgctg gattcactcg
     4441 gggcttcggc gtctggaatg gtcgcgtcct acttcgacgg cacagtgccg gtcgccctgg
     4501 atggcacgaa gctcagcatc ggctttccgg ccgacgccgc tttcagccgc aggaacgccg
     4561 agcggcctga atgtcgccag caactggccg aagcgatcaa ggtcgtgacc ggagatcagt
     4621 ttcaggtcga atacgcaagt cttgacagcg gccaggaggt ggccgacccg gaatcggaag
     4681 tgatggagga agaagacttc atcgcccggg tcaaatccga attcaacgca gaggaggtca
     4741 tctgatggca cgcaagggaa tgggcggtat gcccggtgga atggacatga acaagctgct
     4801 tcagcaggcc cagcagatgc aggccgagat gatgcaggcc caggagtcac tcaaggacga
     4861 agtcgtcgaa gccagcgccg gcggcggcat ggtcaaggtg aagatcagcg gcgacctggt
     4921 tgtccaggac gtggtgatcg atcccgacgc cgttgatccc gaggattcgg agatgctcgc
     4981 ggagatggtc caggccgcga tcaacgaggc cattcgctcc gcccagcagc tggccgaatc
     5041 gaaaatgccc cagatgccga acatgcccgg catgcccggg ctcggtggat gagcgacggc
     5101 ccggcaccac tggcccgcct gaccgccgag ctctcgcagc tgccgggaat cgggcgccgc
     5161 accgcccagc gactcgcctt ccacatactc aggtcggatg aatccgacgc ccggagcctc
     5221 gcgcaggcaa tcgtcgaggt caaggagaag gtcggtctct gtgagatctg tttcaacctg
     5281 gccgaaggtc cgcgttgccg gatttgtgaa gaccactcga gggacgaatc actgatctgc
     5341 gtcgtcgagg aacccgcgga cgtgattccg atcgagcgga cccacgaata ccggggccgc
     5401 taccacgtgc tcggcggagc gctctccccg atcgacggca tcgacgccga ggacctgcgg
     5461 atcgcggagc tgcgtcgccg ggtcgaggcg ggagggatca gcgaggtgat cctcgccacc
     5521 aacccgacca caaccggaga ggcgaccgcc tcacacatcg cggacctgct ccgaacccag
     5581 acgaaggtga cccgcctggc cgccgggctg ccggtcggcg cagacctcga acatgccgat
     5641 gaggtgacac ttggccgggc gatggctggc cgagggtcga tctagatcgg gcctcagacc
     5701 aggacccccc ggcgggcaag ggcagtcttc aggggttccg gttcgatgaa ttcatcgagg
     5761 tccacgggtc gctcgatgat tccgcggtcg agcaggaagt cgtgctgttc ccggagatca
     5821 gcgactttgt ccattctcag gtccacgccg agttgccggc tgacccgagg gctgtaggcc
     5881 gcgtccagga ggtcctcgtg gatgcccgcg tcgagggcga tcaggcgcct cgcttcagcc
     5941 tcttcgtcta ttgaccactc ggcggcggca aggatctgga ccagaacgct ggtgaccaga
     6001 cccggcccgc ggtcgagcag cccgccactt accgttaccg ccagcagcga gcggttgttg
     6061 aggtcgcggt tgactgctct gccgggctcg agggtggtca ggccgaacgt cgaggcgatg
     6121 atcgccgcgg tcgagccctc ggatgcgatc gcatcgacct cacccctgat cagcgcgagc
     6181 agctcattgc tgtgcgcgga ggaaacctga aaggcgacgc cttccctggg ggcatcggcg
     6241 atcggctcac ccggcttcgc cctcgcctcg aagtaggagc cctcgaccgg gattgccacg
     6301 agctccacgt cgttcaacgt gaggccggcc gaggcgagca ggttgctgac tgtccggagg
     6361 gctgtggccc tcgggatgtc gaccacggcg ttcttcctca caggaatcgc gatgcggcgg
     6421 ccgagaaggt ccgctgctgt ccggtagccg gactccggga gggcgagcac cgggtgaatg
     6481 ctcgaggtgc gcgaaaggcc gatcagcctc acgtcgtttc ctcgggagcg ggcgatgagc
     6541 ggagctacgt ttccgccctg gcgaaaggcc ctgggggagg tgcgctcgcg cctcaggcgc
     6601 tggacatccg ggtcagacga ggccgccagg gccctgacct cgactccgca ccgggccatc
     6661 tcctcatgga tccagccctt cctcagggca atcccgaaag cagtgggtga cggggcgtgt
     6721 gtgtaccaga tgtgctcgct cattcggggc attgtagtca aacttgacca agaaagtcat
     6781 aaatgttggc taaaggatga agcggcttgg tcggaccctg gggatcagcc caatgggtgc
     6841 cgtaggagag ccatttggcg cgccttggcg agcagcttgc catcccggct ccagagctcg
     6901 gcatcctcgg tgaagtggcc ttcggttccc tcttcggcgg tcaaccggat caggttccag
     6961 tccggcggac cgggctcggg gatggttcgc gggaagtgca ccgtgtactc cagggtcgga
     7021 gcaaagggaa tcttctcgaa aacgacgaac ggcgccggat accagacgtc gagcaccgcg
     7081 gccgcgagga cggggtcgac cggctgctca tcacgcagcc ggatccagcc catggtttct
     7141 gccctctcac cggcttctcc gggaatgccc ttgatcatca tcgcgcggta acggccgaaa
     7201 accgcgaccg gggcctgctc ggtgtcccat tcctcgagtt cttccggggg cggaaccttc
     7261 ggcatcaggg aattgtcaaa ggccggccct tcacgctcac ccgagaaaac tgccatcgcc
     7321 ttcgctttca cttcaccgct ctggaccatg cgggcagaaa gaaacgcggt cactcttccc
     7381 gcatgctcga ccgtgacctc gatctcagca ggaccggctt cgggccggag caggaagtgg
     7441 acgctgagcg acctgggagg cagtgccgcc tccggttcca tcgacgctac ctcaccggtc
     7501 attgcccgga gcatcaaggc tgcgacatag ccgccgttgg gtccgcgacc aacccaccag
     7561 cggtcactca ggttgatcgc gtagcgaccc tcgcccagcg gctcggccgc cgtgtcctgc
     7621 tcgaacccgg tcggcattct caggccttct tgaccacgct ggacttcagc tgcatctgcc
     7681 cgaaaccatc gatccggcag tcgatgtcat ggtcgccgac accgttgacc agccggattc
     7741 ccttcacctt ggtccccgcc ttgatcggcc ccgaagcccc cttgaccttc aggttcttga
     7801 tcacagtcac cgtgtcaccg tcggtgagca cgttcccgac cgagtcacga atctcggggg
     7861 ccgaatcccc ggcgatctcg gtgcggtcgt gagccggatc ccactcgtgg gcgcaaagcg
     7921 agcagacgga aaaaccgcca agttcatagg tatgttcgct gccgcattcc gggcagcgga
     7981 gagatccgtc ggtcacccgg cgcaggatat tgaaatgcgc ttggggaccc ggttaaccgg
     8041 tgatccgtat gctcaggggt gatgcctgaa agagatgaca tcgtcgtaat gaagttcggc
     8101 gggacctcgg tcgccggccc ggaagagatc aagcgtgccg caaggcgaat ggtcgctgcc
     8161 cgcgaagccg gcaacagcgt cgtcgcggtg ctatccgccc ggggcaagac gaccgatgaa
     8221 ctggtcgcct cggcctacga gatctccgat cgtcccgatc cccgcgagat ggacatgctg
     8281 ctctcgaccg gtgagcgaat ttcctgcgcc ctggccgcga tggcgatcaa cgacatgggg
     8341 cacaaggcgg tctcactcac cggctcgcag gccggaatcg tgaccgactc ctcccatacg
     8401 aaggccaaga tcatcgacgt caaggccgac cgcatccgtg aggggctggg ggaagacaag
     8461 atcgttctgg tcgccggatt ccagggtgta tcaaccgaca gtcgggacgt gaccacactc
     8521 ggacgcggtg ggtcagacac gaccgcagtc gcgctcgcgg ccgccctcgg tgcgtcagtt
     8581 tgcgagatct acaccgacgt gaccggcgtc ttcagcgctg acccccgcat cgtccccgac
     8641 gcgagaaaac ttcctgtcgt ctccttcgag gaaatgctcg acatggccgc ctccggcgcc
     8701 ggagtactcc agctccgttc ggtcgagtac gcccgcaact acaacatccc gatccactgc
     8761 cgaagcagtt tcgaggacct ccccggtacc ctcgtacttc ccgaaagcga aaccatggaa
     8821 cgaccgatca tcacagccgt cacccactcc accgacgaag cccgcgtggc cgtcatcgga
     8881 gtaaaggacg agcccggaat tgccggccgt atcttcggcg cgctagccga tgcgaacgtc
     8941 aacgtggacg tgatcatcca gaacgagccg gtgcacgagg gtgctctcgc ggacctctcg
     9001 ttcacggtcg agaccggtga tctggccacg gccaccgacg cgatcgccag cctgggcgac
     9061 atcagccagg aagtccgcac cgacgagcag atcggcaagg tgtccatcgt cggcgccgga
     9121 atgcgcagcc accccggcgt tgcagccaag gttttcgagg ttctgggcga aaaggagatc
     9181 aacatcgaga tgatctccac ctcgtcgatc cggatttcat gtgttatcca ggccgatcag
     9241 gtgccggaag cggtgcggtc cctccacgcc gccttcgacc tcggcgagga gcagatcctg
     9301 gccgaagcgc cgactggcga acatcgtccc gtaaccccgt cctgatcatg gatgaatcca
     9361 ggtacagggt cggcgtcctc ggcgccaccg gagcggtcgg ctcgaccatc ctcgagatcc
     9421 ttcacgagcg ttcattcccg gccagcgagg tggtggcttt cgcctccgaa cgctcggccg
     9481 gcaagaaggt tccctttggc gagggagaac tgacctgcgt ggttcctgac gacgagtcga
     9541 ttcagggcat cgacatcctg atctcctcgg cgggcggatc cgtgagtgcc gaatgggctc
     9601 cgaagttctc tgaagccggc gcggtggtgg ttgacaacac ctcttactgg cgcatgcatg
     9661 aagacgtccc gctggtggtg gccgaggtca acccggaggc cgccagggac cacaagggga
     9721 tcatcgccaa cccgaactgc accaccatgg tgatgatgct cgtgctcgct ccgattcacc
     9781 gtgaggcgcg cctcgagcgc ctgatccttt cgagctatca ggcggtttcg ggtaccggtc
     9841 agaaggcaat cgccgaactg accgagcagg ccgggcaggt gctggccggc gagacggtct
     9901 cttaccctgc cgtctacccc caccagatcg ccttcaacgt catcccgcag gtcgagacct
     9961 tcaaggacgg cgacgattac acgaccgaag agcgcaaggt gatggccgag acccggaaga
    10021 tcctcggtct cggtgacgag gtgaagatct ccgctacctg cgcccgcgtg ccggtcttca
    10081 ccggccattc ggagtcactc aacatcgaga cccgcgaaga cctgagcccg gaccgtgccc
    10141 gcgagatcct cggcgcagcc gagaacgtga tcgtggtcga caatccgggt gccggcaagt
    10201 atccaaccgc tctcgatgcc gccggccgcg acgagaccct ggtcggccgg atccgccgtg
    10261 accccggcca ggaaaagacc ctgaacctct ggctggccgg cgacaacctc cgcaaggggg
    10321 cagccaccaa cgccgtccag gtcgccgagc tcctggtcaa gaacgactgg ctctagccgg
    10381 tcgggtcgta cctggaaaac aggtctgcta ccgcggccac gaaccccggc aggcccggtt
    10441 cgccccttcg ccccgggctg tccgggccgg cccagcgggc gaccacgtca cgcctcaact
    10501 cgagcatcac ggcggcgatt cgtgattccg actccagaaa cgagagtggc acgtaacagc
    10561 ctctgaaggg ctggtccagt tcgaccgtgt ccggccagag accggagatg gcgtcgacca
    10621 gccactgggg cgtatggaac ttgtcggtgc cgatgcagag cgcaggcctt gcctcgccgg
    10681 agtgaagttc gtaagggagc ggatctcggg gaaacgagtg cacgtcaatc agcagcacgc
    10741 gcccaagacg ctccagcctc tgttcaacca gccgctcgaa ggcgctcgca tacggccggt
    10801 agtactcgga catgagacgt tcatcatcgg cctcgtctgc gcggcgaaac tccgccccgg
    10861 tcgtcgtctt gagatagacg gggcccatac cggcctccag catctcctcg cgatcatcag
    10921 gaaaacgttc gggatcgacc agcaaccggg agtagcggtt ctcgaatatc cagggacgaa
    10981 gttcggcgag tccggcggct tctgcggcga gtcgttcggt gtcggcgtcg gtgatcgcgt
    11041 ccagctctgc gtccaggccc tcccggtcga ccaccagcct gttgagttcc ctggtcggga
    11101 aggccctcga ggcatggggg acgtggagga tgaccgcgct ctccgggtct ccctcggtga
    11161 tcagccagtt cccgtccatc tggtcggccg gtttttctgc gttccagggc atttgcgtag
    11221 agtcgccccg ggctcccgaa cctgacaggg ctctagtcgc cgaagtcttc ggcgatgtcg
    11281 gcgattctct cgtagagcgc ctggaagaac tcgcttctgg gtacgactgc atggacccgg
    11341 ccttcggttg cgatcgggac caccatcacc cggtgatgga ggaaggtttc cgccagctgc
    11401 gtgtcggcgt aatcgtcctc gaccaggacc gggtccgtgg tcaggtatcc gcgcaccggt
    11461 tcctcctgac ttccgatccg tcgctcgatc gtttcgtcga gtgagcgccg gatcatgcgg
    11521 gcgctcatga gggttcccac gtatccgggg aagactgcgg tcatgaactc ccgctcgccg
    11581 aagagcccgg cgtacttgcc gtgctcatcg accaccggaa gagccggcag gccggactcg
    11641 atcaccagcc gcgccgcgta cccgaccggg tcgctggtct tgagcggctt gacctcccgg
    11701 atgaccgaac ggactatgtg gtctgccatt tgcccatctc gcagaaacta gccggtttca
    11761 tcggtcagaa cctcagcaac acgtaggcgg ttgccatgac ggtcgacgcc acggtcaccg
    11821 ggaagccgac cttgagaaaa gtccagaacc cgatcggcct ccctgccttc tccgccatcc
    11881 ccgatgcggc gacgttgcag gcggccgaga tcagggtcag gttgcctccg aagcaggcgc
    11941 caagcgagag cgcccaccag tatgcgtgat cggtgctgcc agccttgatg tcttcgacca
    12001 ccgggatcat cgcggcggtc agggggatgt tgtcgacgat cccgccgacc agtgatgagg
    12061 tccagatgat cccgaccagt tcggcgtccc ggtttccgtt ggtgacgctg gtgattccct
    12121 cggccagctt ctcgatcgcg cctgtcacct ccagggcacc aaccatcacg aagagcccga
    12181 tgaagaagaa gagcgtcgtc cactcgatct tctccagggc ctcctcgatt ggtgttttcg
    12241 tgaccatcag tccgactgcc gctccggtca gggcgatcgt cgccggctcg acgtggatgt
    12301 actggtggag gaagaaaagg atgatcgtcc cgatcaggac cggaagggtg cgccggagct
    12361 cgtcgtggtc cctgatcgat gcggctgcgt ccagttcctg gacgtacttc cggttctcct
    12421 cggccacctg gagctggctc cggtaagcga agtagaggcc cacggtcacg gtagcgagga
    12481 tcaccacgac gccgggcatg ttgttgatca ggaagtcgtt gaagctcagg tttgccgagt
    12541 tgccgatcat gatgttcggc gggtcaccga tcagggtcgc agcaccgccg atgttcgcgg
    12601 ccatcacctc gatcaggatc agtggcatgg ggttgatgtc cagggtgtcg gccagcagga
    12661 aggtgatggg cacgatcaga aggatggtcg tgaggttgtc gaggaaaccc gacatcaccg
    12721 ccacggtggt gaccagcatc accaccagca ggaatggccg gccgcgcgaa agctggccgg
    12781 ccttgactgc gatgtagtcg taaaccccgc tctgctgggt caggtagacc aggatcatca
    12841 ttccggcgag cagaccaagc gtggcgaact cgacggattc gatcgccagt tcctcgttgt
    12901 actgaccaac gaagagcacc acgatcgcgg caccgagcag ggccaccttg gtcctgtgga
    12961 cccgttcgct cgcgatcagg gcgaggacga tcagaaatgt tgcgactgcc agtgccagat
    13021 accgagtcta tgtagtgcgc cggttgaagg cggcgaggtc gtaaatcagg cctgaagagc
    13081 ccggccgagc tgctcgacag tcaccacgcc ggccagtttc ccctggtcgt cggtcaccat
    13141 caaggccccg aaacggcgta gctgggggtt cccgagcagc cggtcgaggc cggcgtcgga
    13201 gcgcaccgaa aagcccggat tgatctcgac cagcgagatc accggggtcg cgtccctttc
    13261 gacctcgggt accgcttcaa gccggtccct gaccgccatt ccgaggaacg agccgtgccg
    13321 gtcgaccacc gggaaccagg accaaccgaa gcggaggaag tactcctcga gggccctggc
    13381 acagtcgatc tcggccggga tcgaaaccgg ttcggtgtcc atcacctgcg agacggtgat
    13441 cccttcgagc ttgtctccga ccgtgctgcg gctggccgcc cgggccgagc tgttgacgag
    13501 gaaaccgatc atcgccagcc agaggccgct gaacaggttt ccgttgaaga tccagagcag
    13561 gcccagcccg atgaagatga acccgaagat gcgaccaaga ccggcagcga accgggtcgc
    13621 ggcgctgcgc ttgccggtga tcttccaggc gatcgagcgg acgatccgcc caccgtccat
    13681 tgggaaggcg gggagcaggt tgaaaccgag aacgaggatg ttgaccgctg ccagccaggc
    13741 gaccatggtg atcgggccgt tggtctccgc ggtgatccgc atctcgaggg cgtcacggaa
    13801 gttgtcgaac ccggccgcct gaatcccgat cacgcagagg atcacggcga tcagcaaggt
    13861 caccaccggt ccggcgatcg ccactctgaa ttccgttccc ggcgactcgg actcctggtc
    13921 catttctgcg accccgccga acagccagag gcggatcgtc cggatgccga tcccgctgcg
    13981 cttcgcctcg aatgcgtgac cgagttcgtg gagcaggatc gagccgaaga aaccgatcgc
    14041 gctggccagg gccagtccgt aggactgggt gctcccgggc tcgtccagga tgtcgccgta
    14101 gaagcccgag agccagacga tcacgatgaa aaggaagatg aaccacgacc agtcgaccgc
    14161 gatccggatt ccggctaccc ggaagagagt tatcgagttt gcgccaccga acacccccac
    14221 atcgtatgga cccgcgtgaa cggggcgggc caagcaacgg ctatcttgct cctccatggc
    14281 aaacgggaca ccgatgaaac ttgccgcgcc gcacggcgaa gcggaccaga actggctccg
    14341 tgcccgactc gaggagctcg agcgaatcga tcgtccttcg gcttcggcgg gggagcgcgc
    14401 tgccgccgag tggctggtca gccggttctc tgaactcgga gcggaagccc ggatcgagac
    14461 cgagaaggcc cacggaacct actggtggcc gatcggtatc gggacggcgc tcggagccgc
    14521 gggcggcatt gcggcgaccc gtggcaggcg cctcgccggt gccctgctcg gggcggccgg
    14581 cgccgcggcg atcgccaccg acttcccgcc ccggccccgc tacctcagaa ggttcctgcc
    14641 gaagcgcgac accttcaacg tggtctgtga actgggcgac cccgaagcgg aacgcaccgt
    14701 cgttttcgtc tcccaccatg acgcggccaa cgccggcctg gttttccacc cgggcattcc
    14761 cgatctagtc tcgaagaccg gcgccttcca gcacctcgac acttcaccga tgctgatggc
    14821 cccggtgatc ggtggcccgc tgctcagcgc gatcggcggt ctgaccggga gccggaagct
    14881 ggcagcggcc gggctcctgc tttcggccgg atcgaccgcc tcgatggtcg acatcggcct
    14941 gaggaaggtc gttccggggg ccaacgacaa cggcacggcc gtgatcgccc tgctcgaact
    15001 cgcccgccgg tttctcgcag accctcccga gggtctccgc ctgatccttc tctcggcggg
    15061 ctccgaggag tcattcagcg agggaatgaa agccttcggg gaacgacact ttccgaacct
    15121 gccggtcgac tcaacctttt tcatcaacat ggacacgatc ggctccccgt acttgacggt
    15181 gctgcgcggg gagggtttcc tgaaaatgtt cgagtacccg cctgaagccc tgatcctggc
    15241 cgatcgggcc gccgaagagg aaggcatcga gttgatgccg aacctgagga tccacaacgg
    15301 gaccgatggt ctggaggcac tagcggccgg ttacccggtg gtctcgatct gttcgtgtac
    15361 cgagcacaag cagccggcca attaccactg gccgaatgac gtttcagaga acgtcgactt
    15421 cgacaccgtc gagtccggca tccgccttgc cgaagctatg gcccgccgcc tctcccggga
    15481 ctgggtcagc ctcccgggct gaacgcttcc gtggcgcccg cgctgacact tcctctccgg
    15541 aacagtgtct atcgccgtct gttttccgcg cagatcgtcg ggctgcttgg aaccggaata
    15601 tcgacggtcg ctctgggcct gctggcattc agcatgggag gaggcaacgc aggtcagatt
    15661 cttggagtgg tactcgccct gaaagttgcc acctatgttc tcgtttcccc ggcaatagcc
    15721 gcattcgccg ccggtgtctc acgaaagaag ttgcttgtca cgatggacgt ggtccgggcg
    15781 ctggccatcg gttcgatcgc tttcgtcggt gagatctggc aggtctacgt cctgatagtg
    15841 atcgtcagtg cggcctcggc ggggttcacc ccggcattcc aggccttgat ccccgacatc
    15901 ttcaccgacg tggacaccta taccgaggca ctatccctca gtcgcctggc ctacgagctg
    15961 gaagctctcg tcagcccttt gattgccggg ttgcttctgg ctgtggtcgg ctacacggag
    16021 ctgttcaccc tgaattccct ggccttcctg acctccgcgg gactcgtgtt gaccaccgct
    16081 gtcccggctg tccgggatga cgccctggcc gccagaaagt tgtctcaggt ttcccagggc
    16141 attcgccagt ttctgcgtac cccacggctc cagggtctct ttctcctcga ctgggccgct
    16201 gcggccgccg gcgccatggt cctcatcaac acggtgatct acgtcaaggg acagcttgga
    16261 ttcggtgact ccgacgtcgc ctgggctctg ggggcgtttg gtgcgggatc gatcgttatt
    16321 gccctgctcc tgccgactct gcttcgtcgc ttcaccgatc ggtcgctgat gctccttggt
    16381 ggcctgatcc tggcggcttc gatgttcctg tttgtcgcag ttggatcgct gacgacactg
    16441 atggcctgct gggcactcgc tggaatcgga acttccgcgg tcctgactcc ttcaggccgt
    16501 ctgatccaga gaagcggcga ctcggccggt cggccagccc tcttcgccgc ccagttctcg
    16561 ctttcacact tctgctggct ctttacgtac ttgatcgccg gcttcgcagg agaagctctg
    16621 gggctttccg aggttgccct gattctcggc gcgatcgccg ccggcgcgag tgtcctggca
    16681 gcgatggtgt ggcggcccga gccgcctgcg cgtcaggcct cgtctacggg gtcgccccag
    16741 cggttgtccc ggtaatcgcg gtcgcgctgg tcctcgacgg ccttgccgtc cggcccgtat
    16801 cccgcttcgc tcagcatccg ccggatgtcg accagctgct ggcggttgat ctccttgccg
    16861 tgggggactt cgaaggtctc ggtttccgga cccacctcca ccttcaggtg gccgttgtgc
    16921 ttcctggtca ccgtgccgac cgcgttcagc agcgattcga ccttgcgcca ctcgatcgag
    16981 tgcttctggt gatcaaagat ctcggcgagg gtgctgctgt ggtggttgct cagaccgtgg
    17041 tcggccatgg agttcctttc gaagttgacg gaaggatgaa tatcacctgc ccacctgcga
    17101 acaggtggcc atggcccgtc ggttatcgat tagaccgggg gagcggagcg ctggatgatc
    17161 gtcttgaaat cggttccggc gggaagggtg ccgaaggcct cgccccagtc gccggccaga
    17221 cgggaggcgc agaaggcgtc ggccacagca gggtcaccgt ggcggacgag catcgacccc
    17281 tggaaggcga gtcccatgcc ttcgactacg cggcgggccc tgatctcgag cgtctcgggt
    17341 tcaccggtga tctcctgctg gagcttctcg acaaacgaat cgagacgtga gtcagcgccg
    17401 gcggcctcgc cgacctcgct gaagaacgcc tcgtaggaag cggggctctt gaccatcgct
    17461 cgcagtgcgt cgaggcactg gacgttgccg gagccttccc agatcgacat cagcgggctc
    17521 tccctgaaca ggcggggcat tcccgactct tccgcgtaac cgttgccacc gaagcattcg
    17581 agcgattcga cggcatgcca cggggcgcgc ttgcagatcc agtacttgag gaccggagtg
    17641 gccaggcgct gaaggagagc ggcaccttcg tcaccggccg cggcctcgtc gtatgcccgg
    17701 gccagacgca tgaccgttgc ggttgcggcc tcggactcaa cacagaggtc ggccagcacg
    17761 ttctccatca gcggctggtc gatcagcttc ttgccgaagg cggaacggtt catcgcgtgg
    17821 tgggttgcct gcgccacgcc ggttcgcatt cctgcgctcg agccgatcgc gcagtcaagg
    17881 cgggtgtggc tgaccatttc gatgatggtc cggatgcccc ggccgtcctc gccgatcatg
    17941 cgtgcccacg ctccccggaa ctcgatttca gatgaggcat tcgacttgtt gcccagcttc
    18001 tccttcagac gctggatgtg gaaccggttg cgctcgccgt tcggggtgaa acgcggcaga
    18061 agaaagcagg aaacgccggc atcggtctgg gcgagaacga ggaaggcgtc gcacatcggg
    18121 gcggagcaga accacttgtg accggtgatc tcgtattcgc cgccggggcc gccaccgttc
    18181 agcggaaccg cgcgagtggt gtttgcccgg acgtcggagc caccctgctt ctcggtcatc
    18241 gccatcccgg ccaggcagcc aggcttctgg tctgccggct tcagcttgcc gtcgtactcg
    18301 agggaggtga agcggggctc ccattcctcg gcgacttcag gctgatttcg aagtgagggg
    18361 atcaccgaga aggtcatcga gatcgggcag ccgacgccgg cctctacctg ggtcagctgg
    18421 gtgaacaggg cagcccgggc cacattggcg ccgattcccg gctctcgcca cggcagggca
    18481 tggaggtcgt gctcgatccc gagcgccatc aactcgtgcc atgccgggtg gaactcgacc
    18541 tcgtcgatcc ggttgccaaa acggtcgaaa gccttcagcc ggggcggaaa ctcgttcgca
    18601 tcccggccgg ccagaagcac ctcgggctgg ccacagattt caccgagggc cttgatccgg
    18661 tcctcggccc agcctgcacc ctccctgttg acggcctcct gaagcggaag gtcgatctcg
    18721 tagaggttga cgttctccag cggagtcgac tgattgaaga ccgtatgggt ctcgtgctgc
    18781 ggagcttcgg cggtttccag catggagtaa tggtctcaca gccccggcgc attgaggcac
    18841 ggataactac ccattgacga ctgcaaggcg tttcgctcta tagcatcgct gcctgcaaga
    18901 gccttacgcc aaaatcgaag ggaagtacca gtcgtgtcga agatcaaagt gaccaacccg
    18961 atcgtcgagc tggatggcga cgagatgacc cgcatcatct ggtcgttcat caaggagcag
    19021 ctgatccttc cctatctcga cgtcgatctg aagtactacg acctcgggat cgaaagccgc
    19081 gacgacaccg atgaccagat cacggtcgac gccgccaacg cgatcaagga gcacggggtc
    19141 ggggtcaagt gcgcgaccat cactccggac gaggcccggg tcgaggagtt cgacctgaaa
    19201 gagatgtacc gctcgccgaa cggcacgatc aggaacatcc tcggcggcgt gatcttccgc
    19261 gagccgatcg tgatctcgaa cgtgccgcgc ctcgtgcccg gctggaccaa gccgatcatc
    19321 atcgggcgtc acgccttcgg cgaccagtac cgcgccaccg acatggtggt cgagggcgaa
    19381 ggcaccctga ccatgacctt caccccgaag gacgagggcg agccgatcga gatgcatgtc
    19441 catgacttcc ccggtggtgg cgtcgcgatg gcgatgtaca accacgacga ttcgatccgt
    19501 gactttgccc gggcttcctt ccgttacggc ctcgagcgcg gctacccggt ctacatgtcg
    19561 accaagaaca cgatcatgaa gcgctacgac ggccggttca tgatcctctt ccaggaagtc
    19621 ttcgatgacg agttcaagga cgagttcgac caggccgggc tcacctacga gcaccgcctg
    19681 atcgacgaca tggtcgcctc ggccctgaaa tgggagggcg gctacgtatg ggcctgcaag
    19741 aactacgacg gcgacgtcca gtccgacacc gtggcccagg gcttcggctc actcggcctg
    19801 atgacctcgg tcctgatgac cccggacgga aagacggtcg aggcggaagc cgcccacggc
    19861 accgtgaccc gccattaccg cgagcaccag aagggcaacg cgacctcgac caacccgatc
    19921 gcctcgatct tcgcctggac ccgggggatc gccgcccggg gccgcatgga tgagaccccc
    19981 gaggtcagcc agttcggcga gaccctcgag aaggtctgca tcgagacggt cgagtccggc
    20041 aagatgacaa aggacctcgc cctgctggtc ggtcccgacc aggagtggca gaccacccag
    20101 gaattcctgg cctcaatcga cgagaacctc cagaaggcga tggcctaggg cccgccacca
    20161 gaaaccaagg agaagaacgt gagttcaacc gccaaggtaa ccgtgaccgg cgctgccggc
    20221 cagatcggtt acgccctgct tttccggatc gccagtggac agatgctcgg tcccgacacc
    20281 aaggtccacc tgagcctgct cgagatcccg gccgccgtga aggccgccga aggcaccgcc
    20341 atggagctcg acgactgcgc cttcccgctg ctcgagaaga tcagcatctc ggacgacccg
    20401 aaggaagcgt tcgacggcgt caacgtcgga ctgctggtcg gcgcccgtcc gcgtggaccg
    20461 ggaatggaac gggccgatct gctcgaggca aacggtggca tcttcaagcc gcagggtgag
    20521 gcgatcaacg ccggcgccgc cgatgacgtg aagatcctcg tggtcggcaa cccggccaac
    20581 accaacgccc tgatcgctgc ctcggccgct ccggacgtgc cgaaggagcg cttcaccgcg
    20641 atgatgcgcc tcgaccacaa ccgggcgatc gcccagctcg ccaacaagct cggccgctcc
    20701 gtttctgacg tcaagcggat gaccgtgtgg ggcaaccatt cggccaccca gtacccggac
    20761 atcggcaacg ccctggttga tggcgctccg gcccccgccc aggtcgatga tgaagtctgg
    20821 gtccgcgagg agttcattcc gcgggtagcc aagcgtggcg ccgaagtaat cgacgcccgc
    20881 ggagcctcaa gtgccgcgtc cgcggccaac gcggcgatcg accacgtccg cgactgggtg
    20941 ctcggcaccc cgagcgagga ctgggtctcg atgggcatcc cttcggacgg ctcctacggc
    21001 gtgccggagg gaatcatcgc cggcttcccg gtcacctgct ccggcggcga gtactcgatc
    21061 gtcgaaggcc tcgagatcga tgaattctca cgcgagaaga tcgacgccaa cgccgccgag
    21121 ctgatggaag agcggaacgc ggtagccgac ctcggcctgc tctaggccca agcagttcgc
    21181 cgtttcatga aagcccgggc cacgacccgg gctttcgtgg ttatggtgga aacatgcggg
    21241 ggaagttccg gaaaccgacc tatgcagaaa ttgtcgcgac gctggcgctc ttcatcgccc
    21301 tcggcggcat ttcgtacgcg gcgatcaaaa tcccgaacaa cagcgtcggc accaaacaac
    21361 tgaagaagag cgcggtcaac tcggccaagg tccgggaccg ctcgctgctc gccaaggatt
    21421 tcaggaaggg ccagattccc cgcggggcca ccggcgccca gggtgctccg ggcatgatcg
    21481 gcccgaccgg cgccgcaggg gccaccggcg ccagcggaat ccccggtgcg gtcggtgcta
    21541 ccggcccgac cggtgcggcc ggggtcactg gcgagaacgg cgatccgggg caacccgggg
    21601 gtgccggacc aaccggtccg accggggtga ccgggcccag cacgaccgcg gtcagttccg
    21661 gggtcgtggt caatctcggg ctcggccccg actacttccc gatcagcggg gcttcctcgc
    21721 ctcagggagc gcccgacacc gtggcctcac tctcgcccgg ggttcccgtc accgcttcca
    21781 acctcgacgt gaaactgacc gccgccccct tcgcgggatt tcctcgcatc gtcagcctcc
    21841 tggatgatgg tatcgcggtg ctcagttgcg tcatagcctc gggtaccact tgccacgcgg
    21901 atacttcagc gctgatcgat cccggaagcg agctggtgat ggtcagctcg gtcctggcgt
    21961 tcccgagcat caccaccgcg cgcttcggct tcacgctcgg gccatgatcc gatcgaaggc
    22021 tctggcaatt tccgggctct gcgggtaggg tcggcacatg gggaaccggg ggaagggcct
    22081 gaagggattc aagtcgaagc ttcgtcggcc gaacttcgcc gaaacgatcg cggtcattgc
    22141 gttgttcgtt gcgctgggcg gcgcctcgta tgcggcgatc aagatcccga agaacagcgt
    22201 cggcaccaaa cagctgaaga agaacgcggt gaccagcgcc aaggtcaaga accgttcgct
    22261 gctcgcggcc gatttcaaag ccggccagct gccggccgga gcgaccggtg cgaccggact
    22321 ggtcggcccc aggggggcga ccggagaaca gggaattccg ggattccccg ggacgccggg
    22381 gacgcccggg ataaacggca tcgacggttt gcccggttcg ccaggccccg cgggagcgac
    22441 gggagcgacc ggagccaccg gttccaccgg tgcgaccggt actggcggat tctcggcgat
    22501 gaccggacgt acctcgcttg gcagctcgac tcagttcttc gcggtttcgg ggtccagtgt
    22561 cgccggcgcc accgcaaccg gcctggggat gattacgccg gcagttccgg tcacgctcaa
    22621 gaacctgatg gttgtcaccg acaccgcgcc cggcaccgga atcacccgga ctttcgtgat
    22681 tcaggaagac ggaatcaacg tgatcagttg caccctgggc ggcattgcca ctgcctgctt
    22741 caacacttcc ggtggcggcg tcatcaacgc gggaagcacc ctgacgatgt ttgacacggt
    22801 gaccggcggc tcggcggctc cttccgaagc ccgctgggga ttcacgatcg gttcctgagg
    22861 cgccggccgt gtagcgtctg gcctgtgagc gaacggggga aagtcgtgat tctgcgcgag
    22921 ctgctgccgg ccgggcgcca gttgctcgag gagaaatttc aggtgacttc gctgggtctc
    22981 gacgccgacc ggtcacagat gaaagccgcg gctgccggag ccgatggcct tgtcaccgac
    23041 ctgaccttcg cggtcgatga cgagctgctg ggtgccgccg gcccgcaact caaagtggtg
    23101 gcgaatttcg gggtgggcta cgacaacgtc gaccttgccg cctgtgccgc gcgcggagtc
    23161 acggtgacca acacgcccgg tgccctgacc aacgccacgg cggagcttgc actcgcgctg
    23221 gcgatggctg cggcccgcga cctgccggca gccgagaagg acctgcggga aggggcctgg
    23281 caggggtggg acccgaggcg atggcgcggc ttcgaactca gcggggcgag catcggagtg
    23341 gtcggtgccg gccggatcgg tggccgcttc atggaactcg ttgccggttt cggcggggag
    23401 cggctctaca cctcgcgcca gcccaagccc gaccttgaaa accggctggg agccgaaaga
    23461 gttgaactcg acgagctgct cgcccgctcg gacctgatca gccttcacct gccgggcagc
    23521 cgggaatccc gccacctgat cgacgaacgc gcgctgaggt tgatgaaacg gaccgcggtg
    23581 ctggtcaaca ccggtcgcgg caccctggtt gattccgcgg ccctgatcag ggcgctgaag
    23641 gaagaccgga tcggcgcggc ggggctcgat gtgtacgagg gcgagcctga tttcgatccc
    23701 gggttcctga ccgcgccccg ggttgcgctg gctccccaca tcggctcggc aacctaccgc
    23761 tcgcgggacg aaatgtcccg actggttgcc gccaacgtga tcgccgtcct cgagggccgg
    23821 gaaccgccaa acccggtacc gggcgtttga tcatttcggc ctgctgaaga ccagcgaccg
    23881 ttcgcccggg aaggccggtc aggatccgcg atcggccttg cgcgaacgcg gaacggtgcg
    23941 gaagacgtgg tcccagaacg gggtcgagac gccgtacccg tagtgatggt cctggaagtg
    24001 atggcgcatg tgatgttcac gcaccttctt gccgatcgat gacttcggta cgaagtgatg
    24061 aaggtagtag tgcgtgtagt cgtagccgag gtagccggtc aggaaaccgg cgaaaaggac
    24121 gaaaccgtac ggggagccga agatcagcat gaacgccgcg taaaagatca gggccagcgg
    24181 gatgctggcg gccgggggca tgaccagccg catcttgtcg ttcggatggt cgtggtgaac
    24241 cccgtgaatg atgaagttga tcttgtcgcc gcccctgaac ttcggcgtcc agtggaaaag
    24301 caggcggtgg agccagtact cggaaagggt ccagatcagg attccgccga cgaaaaggcc
    24361 gaccgtggcg agggccgaga ggccgcgatc gacgccgagc cagaccagca gcgcgatgat
    24421 cggcccgtaa atcagcaggg gcacgaccgg gtggacccgg gagaagaaat tgagcagccg
    24481 gttctcgaag aggtcagggg aagcggcgag cctctcggtg cgttcggacg atcccattgc
    24541 ctgtggaatg gtagcgtgat tggtcaccct aactctcagg agaaacccgc agtgatgagc
    24601 gaaaccgcca ccgcaacaca ggacttcaag gtcgccgatc tttccctcgc cgattacgga
    24661 cgcaaggaaa tccgcctcgc cgagcatgaa atgcccggtc tgatggcgac ccgccgcgag
    24721 tttgccgact cgaagccgct ggcgggagcc cggatcaccg gctcgctcca catgaccatc
    24781 cagaccgccg tcctgatcga aaccctggtc gaactcggcg ccgatgtgcg ctgggcttcc
    24841 tgcaacatct tctcgaccca ggatcacgct gccgccgcga tcgcggtcgg cccgaacggc
    24901 acccccgagg acccgcaagg cgtcccggtc tacgcctgga agggcgagac cctggaggag
    24961 tactggtggt gcaccgagca ggccctgacc tggcccggga cgggcgccga cggttcggtt
    25021 ggtccgaaca tgattctcga tgacggcggt gacgcgaccc tgctgatcca caagggagtc
    25081 gagttcgaga aggccggagc cgtgccggat ccggccggag ccgactccga ggaattccgc
    25141 atcgtcctgc agaccctgac cgactcgctt gccgcggaca gcagcaagtg gaccgaaatc
    25201 gcaaagggca tcaagggcgt gaccgaagag accaccaccg gggtccaccg gctctacgag
    25261 atgctggaaa acggcgagct cctcttcccg gcgatcaacg tcaacgactc ggtcaccaag
    25321 tccaagttcg acaacctcta cggctgccgc cactcgctga ttgacggaat caaccgggcc
    25381 accgacgtga tgatcgccgg caaggccgcg gtcgtctgcg gtttcggcga cgtcggcaag
    25441 ggctgcgccg aatcactgcg cggtcagggt gcccgggtga tcatcaccga gatcgacccg
    25501 atctgcgccc ttcaggcttc gatgcagggt tacgagatcc gccgcctcga tgacgtcgtt
    25561 gactcggccg acatcttcat cacgaccacc ggcaacaagg acatcatcac cgccgaccac
    25621 atggcgaaga tgaagcacca ggcgatcgtc ggcaacatcg gccacttcga caacgagatc
    25681 gacatggtcg gcctcgaagc gacccccggc atcgagaaga tcgagatcaa gccgcaggtc
    25741 cacgaatacc gtttcgccga cggccactcg atcatcgttc tttccgaagg ccggctgctc
    25801 aatctcggca acgccaccgg ccacccgagc ttcgtgatgt cgaactcctt caccaaccag
    25861 gtgatcgccc agatcgagct ctacgccaag gcggacgatt acgaactggg cgtcttcgtc
    25921 ctgcccaagc acctcgacga gaaggttgcc cgtctccacc tccaggcggt cggcgcagac
    25981 ctcaccgaac tgaccgagga acaggccgcc tacatcggcg tcaaggtcga aggcccctac
    26041 aagccggatc actaccggta ctagcccagc aggccccagc aggacccggc ggtcctgggg
    26101 cggccgctgg catccacccg ctgaagtgcg ggagatattc ccattccgcg agatcccggt
    26161 ttctgccgga tagctccccc aactccgatc tcgcccgtct cccgacccag atccgcggta
    26221 ggtgccgcac gtttaggccc atcaagggcc caaacgcgcg gcacctcccg aaacactttg
    26281 tgccggtagg cggcacaaag tgggctttcg ggaccacaag cctcccgctg atgcccgccc
    26341 gaacccgagg tgcaactgca gatgcgcaat cagctaacgc agttggcccg gtgcttcttt
    26401 ggcgggaacg ggaggtcggc gatggcggct gtttcgaggt agcggttctc catcttcttg
    26461 ccgctggccg ccagcagggc agcgccggtc ttgcgcagct cggcccggga gccgtccttg
    26521 cggacccagg gcgccagggt gatgaactgg cgcttggggc cggcataccc cgcgtatgag
    26581 gggtcgtcct cggccagcag cttgcggttg ccgacgtagt tgcatttctt ctttccgagc
    26641 aggccgcgct gggtgatcgc ggtctggccg tcaaagggca ggtcgccgag gttgccgacc
    26701 atgtgcggag tcacgttgta ggtgaacttg actcccgggt cgacgaccga gcgccaggtc
    26761 gaacccatcc agtcgagcgg ctgccagtag gtgccttcgg gggaagccca ttcaccgggg
    26821 ttggcttcat cctgcatgac cagattggtc ccgaggtggc tgaggcaacg catgtaggtg
    26881 acgctgacgt cagcgcacgg cgcgtcggtg gagaccgggc tgccgaggtc gtatccgaac
    26941 cggaaggcgg ggagactggt cgcgaagccg accctggcct tggttccggg gatccggtac
    27001 ggcttcaggt tggcgatcgc gtcggcgccg gtcgcgggcc cggcggtcac ccccagcccc
    27061 tgttcgatcg gggtgagcgg caccttcagg ttgctggcga ccacgttgcg gagcggccgc
    27121 gggccctcct gggtgaccgg cttcggcccc cacatgaagg cctcgttata aacctccggg
    27181 ccggtcgcca cgtaaacgct cttcggtttc ggcaggtccg ggtcccggaa caggttgatc
    27241 tcggccggat cctgcgattc acggaacctc ggctgggtgt tggaaccgac tatgtagatc
    27301 ccgtagcgcc gggccatgtc ggagaaaacc tgcatccagc cgcgggcgtc ggtgtcggcg
    27361 gcggcgacga aaccgcgagt gaacggattc gggatcgagt agcgcgaggt gtattcgccg
    27421 tccggtcccg cgtaggcggc ggtgacctgc atcagcgaat agatcgcccg gcagggcggc
    27481 gcctcaccgg tgcaggaagt gaccgaagag ggtttggcga aagcggcgcg ggccgctgct
    27541 ccccgtgaac cggtgccaag ggtcatcaga ccgacgtcct cgttgaaggc gaccagattc
    27601 ggccggccct tcgccttgta cggcacgacc cacttcctga tcatgcattc gatcttcttg
    27661 cggaaactcg cgtaggtctt gatgtgccgg gcttcctgct tgaactgcat cgcgaacacg
    27721 cgggggccgc ccctggcgtc cttgcggccc agcatcgcca gggcaggccg gcgggtgtca
    27781 cgggcgaagg tcgcgttgca tgacttgagg ttcggggcct tcgggatcgc ggccagcggg
    27841 ggtgcgacct tcctgacctg ggtccgggcc atcaggggat cgttggtgat caacgcgtcg
    27901 actccgagcc tggtcgcttc cctgaccccg gccgcgtcgt caaccgtcca gggaacgacc
    27961 tgaaggccgg cgtggtgggc gtcggaaacg aaggcctggt caatcggcca ctccggtgaa
    28021 acccagtcga tcccgttgtc cctggcggcg ctgatcgcga tcgggttgac cagattgatc
    28081 gtcagatatg aagtctgggg atcggggtcg atcgacttga acgtggtcag gttgtcgatc
    28141 aggaaggact ggatgatcag ctgcgaggaa gggaccccgg agttcttcac cgtgttggcg
    28201 acgatctccg cgaactcgaa ggtggggtcc cagtcgttgt cggtggggag gttcttgatc
    28261 tcgatgttgg ccttggctcc cgttctcttc aaaacctgga gaaatgccgc cagggtgggg
    28321 atgcggggag tcggtcccgg gtagccggct cgcaggttcg ttgcggcgtc gccgatcccc
    28381 ttcacatcga gcgcgcattc ggactcgatt tcgtcaaggg tcttggccga aacggggccg
    28441 gtacaggtcg tggtgcggtc gagggtgtcg tcgtggatga tcaccggcac cttgtccgag
    28501 gtcagtttca cgtcggcttc gaggacgaat ccccgttcaa tcgacgcggc gaagccactg
    28561 ctcgagttct cggcctgggc cgccttgccg ttttcaagcg ggccgccgcg gtgagcgtgg
    28621 atccacggtg aagccgatgc ctcggccgcg ctgaaagcca tcgcgacaag cgccatgacg
    28681 agaatcgaga acctgaacct cacttgaacc ccctctgtct ccagtcgccc cgcgggcgcc
    28741 tctccgtccg tgaggataac cgcggcggcg tccgggcggg cggatcgccc ggtgcatcat
    28801 tctgtccgga ccctggttcg ccccctcggg ccgggtcggc ccgccgcaaa atgcggggct
    28861 tgttgcagac ctgtgaaaca agaaagctgc gatttgcgga atcgctgccg acgcgatccg
    28921 gcggggcttg cttggatggc ccgcggtgtc ccgctcaagc aaaaaggtcc cctcctgcga
    28981 agactgctac ttccgccaga agatgctctg cgcactggat ctcgaagggc cctgcggcac
    29041 cttccgcgag agccggccgg aagggctggt tccgccgcgc cagccgatgc tgctgctgcg
    29101 cccgccccgt tgggcagacc ggcaaatcgg cgccagctaa ccaggcggac ggcgacgaac
    29161 attcacggat ggttcaccgg gctccgcttt cggttgtccg gagtagcatc cgcttgatga
    29221 acggaatcgg gttcctgtca tgaagctgac cgtgctgggc aagtcgccgg cgtggcagga
    29281 cgcggacggg gcatgcagcg gctacctggt caccgagggc gatacgacgc tgcttctcga
    29341 ctgtggcaac ggggtcttcg gcaagctgag gaagttcgtc gattacgtgg atgtcgatgc
    29401 gatcgtcatt tcgcaccatc acgtggacca catcatcgat ctgatcccct acggcttcgc
    29461 cttgaagttc gctccccggc agcaaccggt cccggttgac cggtggcccg ggaccgggaa
    29521 cccggcccgg ccgaaactgt tcgttccccc cggggcgacc ggtttcttca accgggtttc
    29581 ggacagctgg ggtaccggca acctgctcga ggacgcgttc gacacggccg aatacgaccc
    29641 cgaaggcagg ctcgagatcg gcccgatatc gatcagcttc accgaagtcc ctcactacat
    29701 aaccacctac gcgatggacc tgaccggccc ggacggttcc cgcatcacct acggcgccga
    29761 ctgctgcccc aacccggccc tggtcgagct ggcatccggc accgacctgc tgatcgccga
    29821 gtccacgctg ccccgtcccg agcggaccgg agtccgcggg catctgaccc cggccgaagc
    29881 gggtgaacat gcgaagctcg ccggggccgg ggaactcatc ctcacccaca tttcggacga
    29941 gctcgatccc ggctgggccg tcgaacaggc gagttccgtc tacgaagggc ccgtccaggt
    30001 tgcctacgag ggtcttcaag tcgaagtttg agccggaagt gctgcgttac taagcttcga
    30061 ggtcatgtca acgcgcgacc catttgccaa ctttgcccgg atgcgccggg agatggacga
    30121 actgctcggg gggacctggg gccgggccac ctacataagc cggcggtcaa gcggcttttc
    30181 ccccaacgtc gacgtgtatt acagcggcga tccccagcgg gcggtggtca agtgcgacct
    30241 cgccgggatt gatctgggct cggtcgggat cgaggtcagc ggacgcgaac tgaccatcgt
    30301 cggcgagcgg ccggtccagg aaaccgaagg ccgggtgtat caacaggtcg agattccgac
    30361 cgggcccttc cgctacgtgg tcgaactcca ggtcgacgtt gattcggagc gggccaccgc
    30421 cacctatgac gacggggtgt tgcgggttga actgccactc aaggaagagg ccgaggtgac
    30481 ccggcgggtg ccggtcgacc gttcccgcaa ggggcgcgtg atcgatggct gaaccgggcg
    30541 aaagcgtggt gccggtgctc gaagtcgtca gcggcagcga cgaggaagtg gaagaggcgc
    30601 ttcgcggcga gcaggaactg cccgaggcga tgccggtcct gcccctgcgg gacacggttg
    30661 cctatccgga caccctcgcg ccgctcggag tcgggcagga aaggtcgatc aagctggtcg
    30721 acgatgtcct ggccggcaac cggatgctgg tgatggtcgc atcccgcgat cccgaagagg
    30781 acacgcccag tcccgaccag cttcacgggg tcggggtggt cggagtcgtt acccggatgc
    30841 tcaaggttcc ggacgggacg atccgggtcc tggtccaggg cagccagcgg gtccggctgg
    30901 gtgacttcgt gaccgagaag ccgtatctcg tcgcccggat caccgaaatg cccgatgtca
    30961 tcgaggagac ccccgaactc gaggcattga cccgcaacgt ccagaagacc ttctccgaca
    31021 tcatcgagca gatcccctac ctgcccgagg agctccagct cgcggtgacc aacgtcgatg
    31081 acccgtcggc gctttcccac ctgatcgccg gtgctctgag gatcacgacc gatgagaagc
    31141 agcttctgct ggaggaagtc gacgtcgcca aacggctgcg gatgctgtcg gagatcctcg
    31201 cccgggagct ggaggtcatc cagctcggtt cacagattca gtccgaagtc cagtcctcga
    31261 ttgacaaggg tcagcgcgag tacttcctgc gcgagcaact caaggcgatc cagaccgaac
    31321 tcggcgaaga ggacgagcag accgccgaga tcaacgaact gcgtgagcgg atcgaggcgg
    31381 cgaaactgcc ggagcatgcc catcaggccg ccgaacgcga gctttcccgg ctggagaaac
    31441 tgccgccggc cgcggccgag cacggcgtga tccggaccta cctcgagtgg ctggtcgacc
    31501 tgccgtggtc gaagcaatcg aaggacaacc tcgacatcga gcatgcccgc ggggtgcttg
    31561 acgccgacca ctacgacatg gagaagatca aggaccgcat cctcgagtac ctcgcggtcc
    31621 gcaagctgaa tccggactcg ccggggccga tcctctgctt cgtcggcccg cccggggtgg
    31681 gcaagaccag cctcggccag tcgatcgccc gggcgctggg ccgtgaattc gagcgcatct
    31741 cggtcggcgg agttcgcgat gaagcggaaa tccgcggcca ccgccgcacc tacatcgggg
    31801 cgatgcccgg cacaatcgtg cgggccctcc gcgacgccgg aaccaggaac cccgtcttca
    31861 tgatcgacga gatcgacaag atgggctccg acgttcgcgg cgacccctcc tcggccatgc
    31921 tcgaggttct cgacccggcc cagaacgacg ctttccgcga tcactacctg gacatgccct
    31981 tcgatctttc ggaggtcatg ttcatcgcca ctgcgaacat cctcgacacg atcccggggc
    32041 cgctccggga ccgcatggaa gtgatccagc tggccggtta caccgccgac gagaagcggc
    32101 acatcgcccg tcgttacctc gtgccgcgac agatccgggc caacggactg aagccctcgc
    32161 agatctcctt caccgatccc gcgctgaccg cgatcatcga ggaatacacc cgggaggccg
    32221 gggtgcggaa tctcgaacgg gagatcggca ccgtctgccg gaaggttgcc cgcacggtcg
    32281 ccgagaaggg cggcaaggcg aagaaggtca aggtctcggg aaagaaggtg cgcgagctgc
    32341 tcggccggcg ccgggtgttt tccgagacac gacgccgaac cacggttccc ggggtttcga
    32401 ccggtctcgc ctggaccccg accgggggtg acgtgctctt catcgaagca acggcgatgc
    32461 cgggcaacgg caagctggta ataaccggac agctcggaga ggtgatgagg gaatcggcgc
    32521 aggcggcact ttcctacgtg cgggccaatg cacgggacct ggcgccgggc ctcgacgaga
    32581 aatggtttgc cgaacatgac ctccacatcc acgtgccggc cggcgccgtg cccaaggatg
    32641 gcccttcggc cggggtggcg atcacggtag cgctggtttc cctgatcacc gggcggccgg
    32701 ttcgcaatga cgtcgcgatg accggcgaag tgaccctgac cggtcaggtg ctgccgatcg
    32761 ggggcgtcaa ggagaaatcg ctggccgccc agcgggcagg aatcaagatc gtgatcctgc
    32821 ccggacgaaa cgaaggcgac gtcgaggaga ttcccgagta cgagcgggcc gaggtcgagt
    32881 tcttctatgc ggacaaggtc ggggacgccc tggcggtcgc gctcgagggc aatctcaagg
    32941 gcaaacaatg agccggttcg tttttctttg cagcggggta ccgctattct cgacagacaa
    33001 ccattcgatc aagcgaggaa cacatgggaa cagagaacat ctcgggtaag gcctccaggg
    33061 cgtactcgtc ggcgcgcgag aaccagcatg tccgtcgcct gatcgaggac gaagagcttc
    33121 gtgcgagcct gatcgctgcc gccgtggccg gtcgcaaggc ctttcagcgg gtccagtcaa
    33181 accggagttc cgcggtcgaa tcggtgaccc aggacaagaa ggtgaagaag gaactttcga
    33241 gcgcggcgga atcgcttcgt gatgccgccg accgcctcaa ggcgccgccg aagaagaagc
    33301 gccacccgat ccgcaacctg ctcctggtcg gtgtcgtgac cggcggcctg gttctggtct
    33361 tcagtgagtc ggcccgcaag agcctgctcg atgcgatctt cggcgccgag gaagagttcg
    33421 tctacacctc gacgacctcc accgagaccg cccagaccaa cggaaccccg gtcggaaccg
    33481 agtagacccg gttccgcaac cagtttcaaa gggccccggg aaaccggggc cctttctctt
    33541 tgcatcctcg ccggcccgga gtctcttctc cgaagaggac gaaactcgac ccgctttcgc
    33601 gggcaatcgc ggaaaccgcg aggttccatt ttccgccaca tagcgcaccc aactccgatc
    33661 tcgacccggt ggttcaaatc gaagtcggag ttgggtgcgc tatgtggcga aatctccctt
    33721 ctcgattctc cggccatcac ccgccccacg gcaacccaac ccatccatca accagttact
    33781 gcagggcccg ggtcggcggt gttccccaaa aacctccgga atgaggagcg cagcgacggt
    33841 ttttggggga caccgccgtt ccaggccctc caccaccaag cctttggcgt ctggctgcca
    33901 tagactctcc cgtcctttgg ctcgcaggga tgacatgaga aatgtggcga tcgtggctca
    33961 cgtcgaccac ggcaagacga ctctggttga cgcgatgctc tggcagtccg gggccttccg
    34021 gcagggccag gacgtcaacg aacgggtcat ggactcgatg gacctcgagc gcgagaaggg
    34081 catcacgatc ctcgccaaga acaccgcggt ccagtacggc gagatcaagc tgaacatcat
    34141 cgataccccc ggccacgccg acttcggcgg cgaggtcgag cgcggcctga ccatggtcga
    34201 cggggtggtg cttctggtcg acgcctccga ggggccgctg ccccagacgc ggttcgtgct
    34261 cggcaaggcg ctggaggcgg gcctgccggt gatcctcgtg gtcaacaagg tggaccggcc
    34321 ggacgcccgc atcggcgagg tcgtggacga ggtctacgag ctcttcatgg acctcggcgc
    34381 cgacggggac cagatcgagt tcccgatcgt ctatacgaac ggcaagaagg gctgggcttc
    34441 cctcgaggag ggcctcgaag gcgaggacct caagccgctg ctcgacctga tggttgaaca
    34501 cattccggcc cccgaatacg aggagggcca tcctcttcag gcctgggtga ccaacctcga
    34561 cgcctccccg tacctgggcc ggctcgcact atgccgggtg atgcagggca cgatcaaggc
    34621 cggctcgcag gtcgcctggt gccgggccga cggcacggtc gagcgggccg cggtgtccga
    34681 gctcttcgtc accgaagcgc ttgagcgggt atcaacggac caggccgggc cgggcgagat
    34741 catcgccgtg gccggcattc cggaagtgac gatcggcgag accctctccg acccggagga
    34801 tccgcggccg ttgccggtga tcaaggtcga tgaaccttca ctttcgatcg tggtcggaat
    34861 caacacttcg ccgctggcgg gccgggacgg cgacaaggtc accgcccgcc agatccagaa
    34921 ccggctcgat caggagctgc ttggcaatgt ggcgatcagg gtgctgccga ccgaccggcc
    34981 cgatgcctgg gaagtccagg gacggggcga gctgcagctg gtcgtactgc tcgagatgat
    35041 gcgacgcgaa ggtttcgagc tcaccgccgg ccagccgcgg gtgcttaccc gcgatgtcga
    35101 cggccagatg cacgagccga tggagcgact ggtgatcgac gtcccggaag aacacgtcgg
    35161 cgccgtcacc cagctgctgg ccgcccgcaa gggccggatg gagaacatga ctgcccagtc
    35221 cgacagctgg acccggctcg actatgtgat cccggcccgc ggcctggtcg gcttccggac
    35281 cgagtttctg accgaaaccc gtggcaccgg catcctccac agcatctttg aaggctggga
    35341 gccctgggcc ggggacatca cgacccgggc aaccggttcg ctggtcgccg accgccaggg
    35401 ttcgaccgcc agcttcgctc tcttcgggct gcaggaacgg ggggcgatgt tcgtcggacc
    35461 gggcgaagac gtctacgagg gaatgatcgt tggcgagcat gcccgggcca atgacctcga
    35521 tgtgaacgcg gtcaaggaga agcagctgac caacatgcgc tcctcgacct cggacgtgct
    35581 ggtccggctg actccgccga agccgctcac cctggaatcg gcgatcgaat tcctgcgctc
    35641 ggacgaatgc gtggagatca cccccaactc gatccgcctg cgcaaagtcg accttgacca
    35701 gaacgaccgg gtcaaggccg cacggaagct gaagaacctc tcccgctagg agcggggcaa
    35761 cttccgctcc gaccgcggcc ggaattgacc ggcgatccgg gcgccggtta aattgcccgg
    35821 gtgaattccc gtccgacctg ggggcagcgc ctcagatacg ccttcgacaa catgatgtcg
    35881 aggggtaccc cggccctgat cttctggctg gccgcggcga ccctggcgct gatcgtcgtc
    35941 ttcacgatcg tcttcctggt cttcggtctt tcacccgagg gcggggatcc gcacaagggt
    36001 gtgatcggcg aggcctactt caccctgctt cacgcgctcg atccaggaac gatcaccggc
    36061 gacgacggcg actgggtctt cctcttcctc atgttcctgg tcacgctcgg cggcctcctg
    36121 atcttcagcg ccctgatcgg cgtgctctcg accggtctcg atgcccgtct gcaggaactt
    36181 cgcaagggaa ggtcactggt cctcgaagag ggtcacacgc tgatcctcgg ctggggcaag
    36241 ccggtcttca cgatcctcgc cgaacttgac atcgccagcg aaggggaagc ggacccggtg
    36301 gtggtcgtgc tttccgagac cgacaaggtg gtgatggatg acaccctgcg ggagcggtcc
    36361 gacctgaaga acctgcgagt ggtcacccgg cacggcagcc cgatcgatgt cgacgacctg
    36421 gcgctggccc ggcccgagct ggccagtgcc acgatcgtgc tggcgccgct ggaggaaccg
    36481 gagcccgacg cctacgtgat caaggtgatc ctcgccctta cccagctcct tcagggccgc
    36541 gaggtcgagc ggggcatcgt cgccgagatc tccgacccgg ccaatctctc cccggcgcgg
    36601 ctggccggag ccgggcaggc cgtgctgctg gacggccggg agacgatcgc caagctgctg
    36661 gtgcaggcgg cccgtcagtc cggaatctcg gtcgccgtcc aggagttgct cgatttcgat
    36721 ggccacgaga tctacatcga cgatgcctcg cgcttcgagg ggattgactt cggcaccctg
    36781 ctcggccggc tggaaaggtg ttcggtgatc ggcctgctgg acgtggatga catggttctg
    36841 ctgaacccgc ccggctcgaa ggtgatctcg tccggagaca ggctgatcgc cgtggccgaa
    36901 gacgacacgg tgctcgagct tttcgaggaa tcggtctccg gtcccgactt cgagcagatc
    36961 ctggcggccg acgaacggag cccttcgccg gaaagggtcg tgatcctcgg ctggaacaag
    37021 ggggcgcagc cggtcatcac ccacttcgac gccttcctcg cgcccggatc acggatcgag
    37081 gtggttgtcg atcccggcct gggccgggca ccggaagtcg acagccacca gcgcctcgcc
    37141 aataccgacg tgaatttccg acatggttcg accaccgacc gctccaccct ggaggaactc
    37201 gagcttgggc aggccgacca cgtgatggtg ctggcctgct cggatgactt cgatgcccag
    37261 cgcgccgacg cccgcaccct ggtcaccctg ctccacctga gggagatcaa cgacggtctt
    37321 gagcctggct tctcgatcgt cagcgagatg gtggacgagc gaagccgccg gctggccgag
    37381 gtggccgagg tcgatgacgt gatcgtcagc gagaacgtga tcagcctgct cttcgcacag
    37441 atctcgcaga accaccatct ggccgatgtc ttcacccagc tcttctctcc ggaggggagc
    37501 gagatctacc tgcggccggc cgggacctac gtagcgctga acgaagaggt cagtttcgcg
    37561 accatggtcg aggcggcccg ccgccgcggg gaatgcgcga tcgggtatcg ctcggcttca
    37621 cagtccgatg acccggactc gggtttcggc atgacgatca acccgcccaa gtcccgggtc
    37681 ttcaaggtca cacaaggcga cagcctcgtc gtcctcgcag aggaatgaga cttctgttcg
    37741 gagcagaggg ccggccgggt cgttgaatgc aaggcggcga ggcctggggc ctcgccacct
    37801 ccccctgctt ctgtcagtcc ctgccgtgca tccctccctg gcacgcggac tgcccctata
    37861 accgggcagg tggccaaggg ttgcggtgaa attcgaaccg gacaaaaaac tttccgaaag
    37921 gtgttcggca ccgcggcgaa ccggtcaggt tgtggcggcg cctgacggcc tgtcgccgga
    37981 ggttgagcga atgcccgcgg tcaccgaatc aaccgcctgg tcgatcgaga cataggagtc
    38041 ggtcagcagc acctcgaggc cggtcgatct catcatgtcc tcgacctgcc cgacgtcgcg
    38101 ggcgatgacg agccggacct gcctttgatc gagttcctcc cgcagcgcct tcaacatgta
    38161 ggcggcggtc acgtcgatgt agggcaccgt ctccacgtcc agaaccaccg cccgggtgtc
    38221 ggccgcaagg cttcgaatca ccgcctggcg aatctcgtct gaattggcga aatagatcgc
    38281 cgcttcgagt ctcagcacca cgatctgcgg gacctcgacc gcgtttgcgt gccgctgcag
    38341 gtcaccccag tgtccgggct gcgccctgag ctcaccgagc ctggtcacat gcggccgcga
    38401 ggagcggtaa agcagcagca ccagcgaaac cacgatgccg atgaagagtc cgggcaggat
    38461 gtcgaaggcc gtgactccga tcagcgcggc gaccgcggcg atgaagtcgg gccggttggc
    38521 ggggttgagc cggttgccgc ggtccctgat ccgggcgagc tcactcagac cgaccacgtc
    38581 cacggcgtgg atcagggcgg cgatcaccac ggcggcgagg gccgcttccg ggaggtcctc
    38641 gaagagaccg gtgaggaaga gcatcgtgat cacgaccatc agggcgacga cgatgcccgc
    38701 catctggctg cgagccccgg actcggagtt gatcgaggtc ttcgagaaac tccccgtgac
    38761 cacgaagcca ccgaagaggc cggctcccac gttggccgcg ccggtgccga tcagctcccg
    38821 gtcggcatcg agctcctcgt cctcgtcggg ataggccttg gccgcggcga ggctttcggc
    38881 aaagccgacc agcatcagcc cgaccgcgcc ggcaaccagg tcaccgagat cctgaaggcc
    38941 aacgttcggc agggcaaccg aaggcaggcc ggacttgatc tcgccgacga tggcgacgcc
    39001 gtcggagcca aggtcaagca ggctgaccgc ggcgatcccc agcccgaccg cgatcaggga
    39061 gccgggcgcc gacgggtgcc ggtacttgca gatgaggatg atcgccagcg aggtgagccc
    39121 gatcgccacg gtcggcaggt ccacctggtc gaggtgggtc acgaagaccc agatccggtc
    39181 gaagaagttc ccgttgctgg actcgacccc gaagaggtgc gggatctgcc cggcgatgat
    39241 ggtcagggcc aggccgatga tgaatccccg gagaaccgga attgaaatga agttgaccac
    39301 gaaacccagc cgggcgaccc cggccattac cgcgatcagg ccgacgcaga tcgcgagggc
    39361 ggcggtggtc tggacgaagg ccgagctgtc gccggcaact atgccgccga cgatcgcggc
    39421 cgaaagagcc gcggtggcgg cgtcgccccc ggtaaccagc ttgcgtgaac tgccgagcgc
    39481 tgcgtagatc aggagggcgc cgggcgccgc gtagaggccg acgatggggg ataccccggc
    39541 gatcgtcgcg taggccaggg cttccggcac cagcacggcc cagaccgtga gcgcggccat
    39601 caggtccggt ctgagccact cgcggcggta cggccggatc gaaggaaaga gcctcatatg
    39661 cccagcttct cccgggccga gcgcacctga tcgagcgcct ggtcattggt catccccagc
    39721 ccgagcagca ggtcggttgc ggccgaacgg atctggccca cgatcacgct gaccgagaga
    39781 ttcgaggtct cctccagggc ggcggtcgcc ttggcggccg cccgcagggc cgcttcccgg
    39841 gcggtcgagc ggaccggcgc cccgccgagg tgccggtcca gccgggaaac ggaagtagca
    39901 aggtcccgga tcgagtcggc caccaccggc gggatgtgtt cgtcgaggtc gatcgcgcgc
    39961 tggcaggccc gggccagcac gcgggtgtta cggatcatca ggtcaatcgg ttcggcggcg
    40021 gccgcgagct gatcgacgcg cccccgtttt cgtcggcggg tcggggcgag tgaaatcgtt
    40081 tcctgaccgg cctcgacggc gatcttcatt tctcgcaccg ccgggtccag ggaccgggcc
    40141 cgctccaggg cggcgacgat ttcctcgtga ctttcgtggt cgagcgcatc ggcgatgtcg
    40201 accagagtcc ccgaaagcct tgcgagcagc ggttccaggt agcggcgcac caggcgaacc
    40261 ggatcgaccg gggtgattgc gtttgcggcg agcgcaacgc cgccgccgac cagggcgtca
    40321 acgaaccggt gggtcgtgtc ggccaggtcc ggggccggca gggtcgcaac cagcaccccg
    40381 gaaatggctg cctggttgac gagcatcacc ccgccgccgg aaagcactgc cgctgacatc
    40441 gccagcccga tgaccagcat cagctgccag gtgccggtcc cgagcgccag cacgatcagg
    40501 tcggcgatcg cgatgccaag ggccaccccg aatgcgattt cgtaggcccg ccggcctcgc
    40561 tgttcgatcg ccaggcccag caccaggacc accgagatcg gcgcgaagaa cggttcgtag
    40621 tgcccgacga cccgggttgc gatcagccat gcgacgcccg ctgccaggca gatctggatg
    40681 atcctcagcg aggcgaagcg cagggaggag aaccggtctt cgatcagcga tcgcacggcc
    40741 ccactatttc accgggtcgt ccggaaccac gcggtttaga gcttctgcgt ctgctttttc
    40801 gtcttctgca cgctccagcc gtggccggcc ggcggctggc gacggtcagg ctgcaggtct
    40861 gatgatcagg cggtttccgg ccgggtcggc cagttcgcgc acttccccgc cccgcggatc
    40921 atcgagttcg aagcaacgca tgccggccgt tccttccggg gcctgcgggg caccgatccc
    40981 ctgccagacg ttcacggcaa tccggtgggg aaaggtgccg ccgagcgaaa ggtcggcgaa
    41041 accgatcccg tcggcgttca tgtgcgggct gaaaccgatc tcctcgtaga aggtccgggc
    41101 tgcgtcgaga tcccccacgt agagatggat atgaccgatc cgggtgcccg gcggcagcgg
    41161 cccgttcagg tcttcgtcct cgcggtggga gaagacctcc tcgaggccga gcggctcggt
    41221 catgtcgtgg cgacggcctt gggaatcgat gatcagcggc cggccgccga cgatgtcgat
    41281 ctcgccgacc cggtcgatcg tctcgaaggc gatctcgaca ttgatcccgt ccggatcatc
    41341 gagataggtc gcccagtggg tgacgtggtc ggtcggggcg ttggggtagc gggcagcgaa
    41401 gaggcgagca aggatgcggg cgaactccgc ctccgagggc atgtggatcg ccaggtggta
    41461 gagggccgag tagccccgct gaacgggccg ggtggctccc ggatgaagca cgacgagttc
    41521 ctttccgggc tcgtccggct cgcccttgac tcccagccgc acggccccac tctccccgtc
    41581 tccgagctcg accattccga tcaggtcccg ccagaagacc agcgaccttt cgaggtcggt
    41641 cacgttcagg tggacggctc cgtaaaggag attgtcttcg gcggtgctct gaaccggtcc
    41701 gggtcgcatg ccccaactat ggcagccccc ggagtccggt tgaatacctg ctcttgcgca
    41761 accgggcacc agttctcgtc ctgatcggat gtctgattct ctcggccgcc ctgatcatcc
    41821 atgcagggcg cgacctgacc ttcttctttg acgagtggca gctgcttctg gcctccgatc
    41881 cgctgtcgct ttcgaccctg ctcgagcccc acaacagcca gccctccttc atgacttcgt
    41941 cgatctacgg gctgttgctc gagacggtcg ggatgtcatc ccagctcccg taccggttgg
    42001 tcgcggtggc cgcttcgatc gcctgtgtga cgttgctata tctgctggtg cgccgccgta
    42061 ccgacccttg gctggccctg gtcatgatcc ttccggtgct gggactcggg gttgcctggg
    42121 aggctctgct tctggccctc tcgatgaact tcctgatcgg aatcgcgacc ggtctcggtg
    42181 ccttgctggc gctggagtcc cgcagccggc gcggcgatct gctcgcctgc tttctgctct
    42241 gttgctcgct tacctgcggc ggggtcggcc ttgccttcct ggccggagcc tgcgttgacg
    42301 tgctggtgcg ccgggacgct cgcagggcct ggatcccggc ggtcccggtc ctgctctacg
    42361 ggctctggag cttcgccttc ggagatgagg attccccggc cagcgcggac aacgccctcg
    42421 gcctgccgga ctatctctac aagacctccg cggccgggat ccgctcggtc accggccttg
    42481 ccgcggacct gttcccggcc cgaccggcct tctttctctc ggtgttcatc ttcgccctgc
    42541 ttctggcgac ggtcgtctgg cgactcttct tcgccaaacg gccttacagc gaccgggtcc
    42601 tggtggcggg cggggcgctc ctcgccttct gggtgctcgg cggtctcgcc ctcggagaag
    42661 gccgggcccc ggacgcgagc cgctaccagt acccgtcggc gatcctgctg atcctgctcg
    42721 tcgccagcct gcttgacggt ctgaagcttc cgcgctggct gcccgcggcc ctgctccccc
    42781 tggccctcat ttcgctcggc ctgaacgtgt cgcaattcag tgacgggcgg gacttcctgc
    42841 tcaagcagac tgaaatcacc cgggccgcga tcggccaggt cgagatcgag gacccggttc
    42901 ccaacttcag cctggtcgag gaagcaaccg ggagcccgtt ccagcgactg atcgagaccg
    42961 acgcctacct gcgggcagag aaacgcttcg gctcaccggc ctacagcccg gcgcagatcc
    43021 gggcttcggg cccggccggc cgggaggccg ccgggggcgt cgccttcgcc gccgcactgg
    43081 cccgcaagct cgcccacgac aaatagcccg agagccacgc tcccgttcgg cgagatccgg
    43141 cttcttcgtc ataacgacga agaagtcacg cctcaccgat
//
LOCUS       DAOQKN010000002        43072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_319_length_43072_cov_6.051863, whole genome shotgun sequence.
ACCESSION   DAOQKN010000002 DAOQKN010000000
VERSION     DAOQKN010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 43072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43072
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_319_length_43072_cov_6.051863"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /gene="cydB"
                     /locus_tag="PLJ64_00200"
     CDS             complement(<1..854)
                     /gene="cydB"
                     /locus_tag="PLJ64_00200"
                     /inference="COORDINATES: protein motif:HMM:NF014386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HNC91944.1"
                     /translation="MEDTTTLQFVWFVLISVLWIGYLVLEGFDFGIGMLMKLIGGSRE
                     EKRAILHTIGPVWDGNEVWLLVAGGATFAAFPEWYATLFSGFYIALLAILVALIVRNV
                     GFEMWGKRDTDAWRTGWEWCIAIGSLLPALLWGVAWANIIGGVPIEPVTTHGVESIEY
                     TGTFFDLLSVYTLLGGVASLAIFFAHGALFLEIRTEGDIREKARAVAAKATPVAALLA
                     AVFMAWTLIRQDSLEILSLIAAVVAVGSFGFAAINAKSQPMRAFTGTAVGIALYFIAQ
                     FIDLFPNA"
     gene            complement(873..2318)
                     /locus_tag="PLJ64_00205"
     CDS             complement(873..2318)
                     /locus_tag="PLJ64_00205"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNC91945.1"
                     /translation="MSTIAMTSLELARWQFGIVTLYHFIFVPLTIGLAFFTAWYQTRW
                     YRTKDEKWLRATRFWGKLMLISFALGVATGIVQEFQFGMNWSAYSRFVGDIFGAPLAM
                     EGLAAFFVESTFLGLWIFGWGRLSEKVHLACMWAVSLSTMLSAFFILAANSWMQHPVG
                     YELNQATNKVELKSIWDVLTNSTALYAMTHTMLAAALTGGMVIAGISAWHLLRKNEVE
                     VFSSSMKIALPSLAVAGLLTLVVGHFNGILMSEQQPMKMAAADAVFETEDGVGLSLFA
                     TGKFEGNPEETNRNIKLPNALSFIMTGKPDSEVKGVNNLNEEYRQRFGDPASPEYRGP
                     EFKTMDYAPIVWVAYWSWRVMLGCGFLLALFGVIGWWFNRKGKLPDSRRFLKLAMPAA
                     SLPFIAAAAGWIFTEMGRQPWVVFGLLRTQDANSPTVSATEVWISLVGMTLLYGVLAV
                     FAGKIFFKTAGKGPAEIEEGEEDKPYLGMAY"
     gene            complement(2425..3189)
                     /locus_tag="PLJ64_00210"
     CDS             complement(2425..3189)
                     /locus_tag="PLJ64_00210"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HNC91946.1"
                     /translation="MKRLATAAAAVGLVAALGVLAGPVAEHAGSSASANGVAAGPAAG
                     AAPPGAARQSNRAALRMPAWIHRHFIPFGAKRKHQMARYSLRHYGVRSWRLRNPRQIV
                     EHVAVGNSVAGVYRTFAANRPDPEFHELPNVCAHFVVSGRGRVVQLVPLGIRCRHTVG
                     LNHVSFGIEHTGFTDGDVLGNPRQMRASIRLTKWLRCRYGIGIRDVIGHNESLGSRFH
                     RELVPAMKNQTHGDFRHASMRRYRKALHDAGRCPRA"
     gene            complement(3210..4334)
                     /locus_tag="PLJ64_00215"
     CDS             complement(3210..4334)
                     /locus_tag="PLJ64_00215"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNC91947.1"
                     /translation="MTVRSIPRTFARFSLPAALTLICAIAAFMLAGDTARAANGGAVP
                     TGGPARATVKIKLKGVHAHRATVMSRVVIQGTLKPFRNLQTVKVYLYKDGEKFSTRKV
                     KVHKAGGNYGTFRTYLYMRGGNRYGVHAKYWGKAGKRAVGKDTTRHKSWRVRYVGLPH
                     DQCGRVVAGFRKALNRLALVPDQGRCFNGKMERAVLAYRKLNDMTRNARASKTVVRRV
                     FNGKGGYHVRRPELGDHMEAPLSKQVIVFSKGDNPYAIFPIASGKASTPTILGTFSFY
                     WKEPGMNSHGMYYSSYFIRGYATHGYADVPDYPASHGCLRTFIADQPRIYNITEIGMP
                     VYTFGNAFRGKVAGYKVKHSGNGLGPDLGPTGGLDPAAFD"
     gene            complement(4389..5975)
                     /locus_tag="PLJ64_00220"
     CDS             complement(4389..5975)
                     /locus_tag="PLJ64_00220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC91948.1"
                     /translation="MSKYQHLITDENRKWWTLGAMCLALFMVMLDNTVVNVALPSIQR
                     DLDTSISGLEWIVNGYTLSFAVLLAVGGRIGDIFGRRKAFTFGVVIFTVASATAGFAP
                     SNFALVASRVLQGVGAAFMMPGTLSIITDAFPAHERGKAIGTWAGVSALALAVGPVLG
                     GFLVEHVSWRAIFYINLPVGVLAVLAATFVVRESRDTTVGRKVDYLGVGILTVALTSL
                     VLALIEGNTWGWDSTAVISLIAVSIIGLITFIFTENRVTAPIIEFGLFKSRNFIGSVV
                     VAFIISFAMMGVFFFLALYMQNILGYTALEAGVRFLPTTLVIMVIAPIAGRLADRIGP
                     RWPIVVGMTLISLSLYLFSQISDGSTFSDILPSFVILGAGIGLTMSPMSTAAMNAVPK
                     TKAGIASGVLSMFRMVGGTFGVAALGALFQSEAKTRLASTLGGENGLTASQRSDFAHQ
                     LTGGAPHIEGISGAKAERLVAAGHDAFIYGLSNAMTLSVGIAIAGVLVAFFLIGNQAE
                     SMDEETREAGEVAEAASPVA"
     gene            complement(5972..6577)
                     /locus_tag="PLJ64_00225"
     CDS             complement(5972..6577)
                     /locus_tag="PLJ64_00225"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNC91949.1"
                     /translation="MTDAEALKIDEQATAGATDPARGPGVDPAEVAGQEVIDAASPEA
                     KRVALQLGTLFRRMLSYGAATSALLDAAALGLSFLEFKAIMSLGVFEPADSFCLADIG
                     ESTGASMPAVSRAIDSLVRKGLVSRVEDPHDRRRRLIALTDEGHRVTNAALMGRAVGA
                     VRLADSFSEDELKALDSPLSKLIEREDLASIHRQLEGAVQQ"
     gene            complement(6642..8891)
                     /locus_tag="PLJ64_00230"
     CDS             complement(6642..8891)
                     /locus_tag="PLJ64_00230"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNC91950.1"
                     /translation="MDTSRNIAARAGRWSATHRKTAIWGWLAFVILAVFIGGSVGTRT
                     LTDAEDGVGESGRAEVATSNNFPENATERVLITAPAGGSNTSAAMREAAADLSRELRA
                     TPYVSHVESPFAPGNRGQISADGKSALVSFELAGNEDQLSERLGATQEAVNEARARAP
                     GYGFAQFGDASASEQIGQAFEDDFKKAEFTSLPVTLLILVFAFGAFIAAGVPLLLGLT
                     SVAAAIGLTALVSHVTPMSDAANSVILLIGLAVGVDYSLFYLRREREERAAGRSESAA
                     LEAAAATSGRAVIVSGITVMIAMAGMYMTGDATFASFATGTIIVVAVAVLGSLTVLPA
                     VLAWLGDRVDKGRIPLIDRLRRSRKIRRVGGEESVLAAGNGGGFWGRFIRTVLRRPGL
                     SAVAAAGLLVLLAVPAFKLHTVVPGAEALPQDLPVIKAYNQMDEAFPGGPEPANVVIE
                     ADDVRAPEVQQGIAALRQAVDGPAEFGSPVTTTVSPNGKVEVVNVPLAGSGTDDTSND
                     ALVKLREEVIPETIGKVPGTETNVNGMTAGSKDFNDLMKSRAPLVFAFVLLTAFILLM
                     VAFRSIVIPATSILLNLLSVGAAYGLLVWVFQEGHLEGLLGFESMGGITAWLPMFLFV
                     VLFGLSMDYHVFILSRIREAHDQGLSTSDAIEAGLRRTAGPVTSAAIVMVAVFSIFAT
                     LSMLEFKQMGVGLAAAVLIDATIVRGVLVPASMKLLGKWNWYLPAWLEWLPKLNLEGT
                     PEPAPRPAV"
     gene            9055..10812
                     /locus_tag="PLJ64_00235"
     CDS             9055..10812
                     /locus_tag="PLJ64_00235"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNC91951.1"
                     /translation="MGSALNLRARLTWLAIAGFLAGAGGATMIALTNQTLGEPVIEIT
                     LRVLTGWTWMGVGIFAWWRRPDNPFGKWMVFTSFAWLAQALTYANSSLVFTVGLVIAY
                     VFPFALIQTLVRFPTGRIETRADGWIVRLVAGALLLVLLATFFSSPDQLGYDNAPSNL
                     LLLADSPTTSAVFFIIANAVAIILVLGLIHSFTTKYSAASPPMRRLIGPVLWTSTTLL
                     AVLGLSSVFSIANLDTVEEIAGILGAIIAMLLPLAFLVGLARSRLVRGEALGRMLERV
                     AHESDPVKMQEAVAEALGDPSVEIAFWLPDTAGYVDARGLPIAEPDPDSNRAASQVTM
                     DGRRVALIIHDGALLEEPEKIEAVSRGAALAFENARLEAEVRANVAELRASRTRIVEA
                     GDAARREIERNLHDGAQQNLVSLALKLQLARSRVESDPRGAIEIIDGASAELEQTLAE
                     LRELARGIHPAVLSDRGLDAALLALATRAPLPVEVGEMPDDRLPEPVEAAAYYVVAES
                     LTNVARYSEASRAKVDITRENGVVRIRIADDGVGGADPARGSGLKGLADRIAALDGHF
                     MLASPDGLGTIVEAEIPCG"
     gene            10803..11456
                     /locus_tag="PLJ64_00240"
     CDS             10803..11456
                     /locus_tag="PLJ64_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC91952.1"
                     /translation="MRVVVADDSILLREGVVRVLEDDGFEVVGQAGDAEDLLRKVSAH
                     KPDVAVVDIRMPPNLTDDGLQAAKKIRAEHPDTGVLVLSQYLEESYALDLVADSAEGV
                     GYLLKDRVAEIDRFTESVRRVGSGGSALDPEVVSHLLGRRRREDPLEVLTVREREVLG
                     LMAEGRSNIAIAENLVVTERAVEKHVTSIFSKLDLTPTAEDHRRVLAVLTYVKAIGD"
     gene            complement(11526..>12650)
                     /locus_tag="PLJ64_00245"
     CDS             complement(11526..>12650)
                     /locus_tag="PLJ64_00245"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HNC91953.1"
                     /translation="DHFPPEWADEAALGKQGRGGIGSIAERFRRRTGRRPILLPAMAG
                     FAGLLLVATVVVSAVDSGRFGGGSGGEGDSGVVASKQLDTSASSASESGGISAGSAGT
                     ADSATLPVPEASTGAVYGALPSSAMKDQASENSRSSTVPGGSRQRDVALEAEIVLGTD
                     PDGVQDVANEVVSVTDEHNGIVMDSSVTDGEAGNAGASFSLLIPSGQVEEAVADLSGI
                     ADLKSRSQELTDITAPTNRTEDDVADSKAKIESLLSELEDAPDEATRQELEQKLANER
                     SELSYYESRLKRLERRADYTPVDLRVETGGESSSGGDSSSWGLGDAIDDAGKMLGVAA
                     GVTVLALAVAIPIGIVLLIALALNGAWVRRSRRRVLADEE"
     assembly_gap    12651..12750
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12910..13473)
                     /locus_tag="PLJ64_00250"
     CDS             complement(12910..13473)
                     /locus_tag="PLJ64_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNC91954.1"
                     /translation="MEAVPEPVSSPGPKSGPRPLFEDLYRGSRDDLYSYVAGLLRDRV
                     AAEDVTALAFERAFRKWDRYDPKRGSARGWLFGIARNAALDELRKRKREVPAIEPGGL
                     EAPGSVTGAVLEREQRADLMAALGTLAPADREMVALKFFAGLTNTEIAEATGYSSTNV
                     GTRINRAMTRLRSALTDGSASDEEVAR"
     gene            complement(13825..14004)
                     /locus_tag="PLJ64_00255"
     CDS             complement(13825..14004)
                     /locus_tag="PLJ64_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HNC91955.1"
                     /translation="MSTATTQLWICESCGFIYDPAEGDPDGGIPPGTAFEDIPEDWFC
                     PVCGARKADFEPYEG"
     gene            14108..14767
                     /locus_tag="PLJ64_00260"
     CDS             14108..14767
                     /locus_tag="PLJ64_00260"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNC91956.1"
                     /translation="MNKFPEAGPGESINLGKPSEPRPAATILLLRDREQLGTPEKGVL
                     EVLMLKRSAESHFMPSVWVFPGGSLDPEDGDGLDGLRTCAARELDEEAGIKLDLSSEL
                     IPLARWVTPEVVKTRFDTWFFLARAPEGVEVKPDNFEMTEALWVNPSDALAAAEAGDM
                     AIVFPTLKQLEALAPSANTEEAMGRAAADPNASKVVLPKVIGDEQNPRVVLPNEDDYP
                     D"
     gene            complement(14774..15442)
                     /locus_tag="PLJ64_00265"
     CDS             complement(14774..15442)
                     /locus_tag="PLJ64_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91957.1"
                     /translation="MGKQADQWQQIVSSYKGAGEVKWEPVGGINPVGAPVALCVGGSN
                     RLTGELAPDFWGASCDADEEEVGGFMRKELVPTAILAKAHMPDLAQVVPAFNVESIET
                     SELVKQKVSRRKVEFESVDFNKRFLATVPKEHDPIALRELFSPGFLEWTTTIDREVDF
                     GINDRQLWFLWRLRERSAEELKLALANAGELFKRLHSEMDEDGIHTYPGGPWNAGLEP
                     FPAD"
     gene            15526..16092
                     /locus_tag="PLJ64_00270"
     CDS             15526..16092
                     /locus_tag="PLJ64_00270"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNC91958.1"
                     /translation="MSGALIALIVVVVIALIAVLWYIATRNGIIASRNRVEESWSGID
                     VQLKRRHDLVPNLVETVKGYATHEQKVFEEVTKARADAMKAQSVDETAQAESGLNRSL
                     ADLRAVAEQYPDLKATENFQQLSRNLSELEDEIQASRRIYNSNVQAYNTKIQQFPASV
                     VANQGGFTAKEFFEIEDAAERATPNVSF"
     gene            complement(16152..16853)
                     /locus_tag="PLJ64_00275"
     CDS             complement(16152..16853)
                     /locus_tag="PLJ64_00275"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC91959.1"
                     /translation="MPTSMERRHNRRRRHERTRQELIEAALNQSAAGSFKDLTVEGVT
                     REAGVARSAFYVYFGDKEELLLGALEDLIASHQNRLGRCWQQGDDPRRDIERGIYGMA
                     RIYAGNSALLALAGEAATYDEEVRELWSTVLETTTEGTREEIERLQKAGSVNESLDPE
                     ALAEGLVLMTERSFQVHLAQGEDDPNEVASSLTRVWWATLFGPPLPSSPPEGEGGPQG
                     SQPGPEGRGMGDPEK"
     gene            complement(16905..18674)
                     /locus_tag="PLJ64_00280"
     CDS             complement(16905..18674)
                     /locus_tag="PLJ64_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91960.1"
                     /translation="MGGLAALVAFFAVAVLAGPPARAATNEELSSCSEIKQTIEQPDG
                     SIVGMGDCRYGVSPAPRIVLVGLNPDGSDRTAFADGGAALISVEENEFGLKIIGRPDG
                     SFLVLSQKRIWSFDGDGDPVESFGNHGVLNFSAADQMNLASAFDFALDSGGNLLVAGR
                     RPSTATPVVGKYDQTGQLVSGYGNSGVSAELDLNFDAAIAVDNSNRVVMVGEDGATRL
                     LADGSLDLSFGPDNVGYADLPAGLPYSSGEVFGLTVHPDDSITAYLDRTPGMYYYPAY
                     AVSLDSDGSPAAEDALVALGDHVSAVAPFDGGVAFVRFPGRGFQHWQFEVGTTNWPGI
                     RSVTFSPGDSLAHGVTALSDGSLLVSGNAQGPDCSDSCVFRNHLALAKIDPGTGDLVE
                     SFGSGGERLIPPNGCSRGQAGKAGDWNICRVRPPGINGSVRFAGAGSKHPSLVLKASL
                     GDPPEGLVGTRQRLTVDLPRKLRLRPGGLKGRVRVSVSPATPRTVKVQGKRIAILAKP
                     EYNPYETAGESDAKDSRLTFRITVNRGALAPITRPGRLHKRVFGLRGEFNPVWNGFPT
                     PEGGPWFSPNSAFRRIKAAAPSS"
     gene            complement(18741..19574)
                     /locus_tag="PLJ64_00285"
     CDS             complement(18741..19574)
                     /locus_tag="PLJ64_00285"
                     /inference="COORDINATES: protein motif:HMM:NF012355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HNC91961.1"
                     /translation="MRGGSARKLRVALVAVAAASAAFLFAAPATANNTWVSISDFQWS
                     DKNPHIDLGESVTWLWAGPDTPHSVTGQAPRDSAFDPITTTDASQWDSDAGRNEPNHW
                     PGDEYTITFDHPGTYLFACKLHSVVRGEVTVSNLPGNPDSSRGPRPKINFDFDSPVVD
                     DYFFTADGESPAPPVVGPRGRGIGFRFSVNEATTASADYYQLVTRGKGSRKRLVRVYR
                     GYGEWPAHVGINRVGFARPTANFKPAPGRYLAWFRAEDRVQNSTGDIPLRFTISPGKG
                     G"
     gene            complement(19577..20446)
                     /locus_tag="PLJ64_00290"
     CDS             complement(19577..20446)
                     /locus_tag="PLJ64_00290"
                     /inference="COORDINATES: protein motif:HMM:NF012355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HNC91962.1"
                     /translation="MVHRRLTSGAGRALRALIAVFAAAVVASAFTAPASANNTRVTIA
                     DFHWSNKNPEVDLGESVLWTWTGPDTQHSVTGQPAGNSGAESGLPGIGTVATDDASQW
                     DSDPDTNYPIHYPGSEYKVTFDHPGKYLFVCKVHASVRGVVTVSDTQGNPDSDFGPAP
                     VINFDTEGPLIDTYFFTSNGTDPTMPFIGHKGKGIGFSFGTNEGGSASADYYRLVKRG
                     KGKKARYVKFFQGYNEWPTHIGMNNVRFAARSSTFKPVPGKYVAYFRAEDGKSNSSDD
                     ITLNFTVFPKKKR"
     gene            complement(20428..21453)
                     /locus_tag="PLJ64_00295"
     CDS             complement(20428..21453)
                     /locus_tag="PLJ64_00295"
                     /inference="COORDINATES: protein motif:HMM:NF019352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HNC91963.1"
                     /translation="MDRRNFLGLAGGAFLCTIAGKQYSIDSNTDLAALANKIEVPPKV
                     AAAKRSNSVSNADISGNRKEYWIQAEPVTWNVVPKQRDQMMGTSIKKTVGRTKVPAYA
                     YRLYDTDFSKPLGPATVPGPLLDVTVGDTLVVNFRNACDVPVTMHPHGVFYSNEMDGA
                     YKGYWTDPGGFVQKGRTFQYVWECPEGTQGSWLYHDHGPFDSTAVFKGLFGPLIVRDP
                     DEPLPDREYFLGFHSYLPGLFPLKAPLFAINGRAYAGNTPTMRATVGDDVAMHVYGVD
                     NDMHTFHVHGHRWQEAGGTYADNKIFGPGDSFRVRWIEQNPGRWLYHCHVFSHLVTGM
                     SGWYIVD"
     gene            complement(21500..22063)
                     /locus_tag="PLJ64_00300"
     CDS             complement(21500..22063)
                     /locus_tag="PLJ64_00300"
                     /inference="COORDINATES: protein motif:HMM:NF024864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HNC91964.1"
                     /translation="MSSKIKSRAAGLLSVGIVALALGAASAVPATASASGDGAASSSS
                     LTAKQKKAKRAELKKCYRIKAKAKRKACIKRVNKKYAKLAKPPVKVAATIDVLDDYYY
                     PSDVTINRGEAVKWVWSNSNANGHTVSLDDPFPVGLTASEKYSLNTPTAPATFFTFGP
                     KQLKKPGVYHFVCELHSATMKMTVTVK"
     gene            complement(22117..23826)
                     /locus_tag="PLJ64_00305"
     CDS             complement(22117..23826)
                     /locus_tag="PLJ64_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91965.1"
                     /translation="MKQRLAVIVGILAAFLVGAFAVTAPASAADAGAVKLQKQVKAAQ
                     KKATATCKKVKKAKKGKSKAKAKKRCKSAKQNVTRLQKKLKAYNAAQYFDVCKHGCKY
                     RTVQKGADAAGLWQKKSKRKATVRVKPGTYVEGVFLDGRNPKYDYDGLTIMGTSKNAK
                     DVILEGENAKTIVDGVPQVANNAIEGRSIIGLVMKNMWARHYQNNTFFVWAGHDGTER
                     CADYTMDNLVSSDTRSYGFFARNCFGGVIENSSGYHHGDSAVYIGETPCDSPDWTNRT
                     VAAERTPCQADPDWTIIRNVKSYANVLGYSGTNSKYVDIKNSAFYNNGAGIVPNTLDS
                     EQFEPSGWLKIHNNDIFWNNYNYYSTGSDFQTVATIGDVNYPIGIGVVLYGTDSVEVY
                     DNNIFGNEKWGAATFSGPELFGVNVGDDAMNLNNKFIDNNMGRNGDDPNGKFDFFSDY
                     SGGGNCWQGNTAGSSFAPGNGTVALSTIYPSSCPQPVVGQKDVSPIELTAGLQINIAS
                     YADDGDPSNGPGDGDTIFGYVSQHPPQKQECEMNVSTPHPAYTDAKGVSYTEDRADPV
                     TCS"
     gene            24060..25787
                     /locus_tag="PLJ64_00310"
     CDS             24060..25787
                     /locus_tag="PLJ64_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HNC91966.1"
                     /translation="MAHDYDYVIVGSGFGGSVSALRLAEKGYKVAVIETGRRFEDKDF
                     AKTAWNMRRYFWLPKFGLRGILRMSMFKDVFVVSGCGVGGGSLGYANTLYRPRPAFYT
                     DPQWDGLADWEAELGPHYVTAERMLGVTLYRDTGPADALLHEYADEIGADKTYSNTQV
                     GVFFGEAEKTVPDPYFGGEGPERTGCSKCGSCMVGCRHGAKNTLVKNYLFFAEKLGVK
                     VFPGTEVTSVRPIGGPDGEHRDGSAGYELRTDRSGAVLRHRRRKLTAAKVIFSAGALG
                     TNKLLANCLHNGDLPNLSKRVGHVVRTNSESIQAVTAHDDERDFSDSVAITSSIYPDP
                     DTHIEVVTYGQAGDVMSRLYTIYTGNGTRLTRPIRFLATMARHPIDTLHLLFMPRHWS
                     RRTVILLVMQALDSAMRLKPVPKKFGKGVRLQTEQDPERPNPTFIQAAEDATKWFAKR
                     TGGIPQVSFGESLFNIPTTAHILGGAVVGHSPEDGVIDKDNHVFGYENLMVCDGSAVP
                     ANPGVNPSLTITAMTERAMSKIPPKDPAAELLHLPDEARPARTADTVGPARGPGPSGS
                     AESWTPLEP"
     gene            complement(25817..26842)
                     /locus_tag="PLJ64_00315"
     CDS             complement(25817..26842)
                     /locus_tag="PLJ64_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91967.1"
                     /translation="MIGLPTRTAVLPIPPKQLFFLALLAFAGLGMLISGCGSSESGDE
                     VARGAPSASDFPSAEGKTIEELLNDQTPSDKVVSPAAVVFEPGENRYPFGVFNVDRSQ
                     VQDADVAIYVAKSPQAPVEGPMPAEVVSLETKPAYRSKGAGGPGEANSFYLSHVKFDR
                     NGPWLAIAILKTADGFEAARVTPSPVVGQYPDVAQVGQQAPKVSTPTAEDVGGDLSKI
                     DTRQPPSSMHDDDFKDVVGKKPVVLIFATPALCQSRVCGPVVDVAEQVKDEVGDGVVF
                     IHNEVYNENDPSKGIRPQLTAFGLQTEPWIFLINKDGTIEKRIEGAVGVDELKSDVEQ
                     LKKKNGL"
     gene            complement(27014..27613)
                     /locus_tag="PLJ64_00320"
     CDS             complement(27014..27613)
                     /locus_tag="PLJ64_00320"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNC91968.1"
                     /translation="MAYEVPDLPYAYDALEPHIDEATMKVHHDKHHQAYVDKANAALE
                     GTEWADKPVEEVIANLSSLPADKQGPVRNNAGGHYNHSLFWEMLSPDGGGAPSGDLAA
                     AIDSAFGSFDDFKEQFKAGGIGQFGSGWVWLVKDGDGVAIVSTANQDSPLTDGKTPLL
                     GADVWEHAYYLKYQNKRPDYLDAFWNVVNWDYVAERFAS"
     gene            complement(27688..29337)
                     /locus_tag="PLJ64_00325"
     CDS             complement(27688..29337)
                     /locus_tag="PLJ64_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91969.1"
                     /translation="MERGFLRGIVVAASLVFGVMAVSSPALALTPPPVDKASVVFSNG
                     KRLIRIKADATDREVLTLKNSFVSSIDGLHDTALAGSPDGKSVLFRREDWSDRPTGPQ
                     GLMSLMVDGGKPKLLVKQGSLSWIQAATFSADGSLIYFTKTRYAGDWESYTLSVNSIR
                     PDGTGLETIWSAPPVDLDDDEYSIGGITPAPDGRLLLTFDYDTSDSHLDLLDPDTGEV
                     TPLKAGAAWGSYSPDGSRIAFINMKQGYGALSVMNADGTGETRIAPKVPGFIDRGPDW
                     SADGSRIIFQSVRDGWVASYGEEVWAVEPDGSCLTRLTNGAPESTAPVWLGTAGDSFE
                     PGGCGDRAPNAIVETSIPKRVRGLDPPRFWMGPEWEGMLYDDLIRIMNGVSFGYSDCT
                     EFDYTQCAGYREVSTDAVCSYPFFPENYAGMRRVRGTVIVRQNGDYRNEATVYTGGLS
                     VDIQINHAKSFADFVEAARLIQPVSGPRLHRLEGPVFTLGDVRTAHLVLRTKRQEGSF
                     ASLRRLGISPREARGYLRFARDLDRIGKYGTTRCRGFSNLP"
     gene            29632..32856
                     /locus_tag="PLJ64_00330"
     CDS             29632..32856
                     /locus_tag="PLJ64_00330"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNC91970.1"
                     /translation="MPNSIAVLGDSMSVATGINVIPSVTQPESSWSTGTNSSVNSLYQ
                     RILALNPAISGKNRNMAANGEDMTDAPAQANATPADTELVTMQLGGNNLCKDSVAQMT
                     SVAEYRARFVETLDILQSRMPNALIQIESVPDIYNLWFLRGAPNPPNDQPSSRAGTAR
                     VFWDTLAVIPCKSLVLDPTDMSQAAIDRRNAVRQRDLDYNKALAEECEARLRCRFDRY
                     ATFAFSSNRVVPGDLTGPYLPRAQWQFVDDDISTVDHFHPGLSGQRKMAQAAWESGYN
                     FTDSTMPTGAASISPAPLQSGTSFLPSTVSTTWSDSAGIKGIQYRTHFNGTASAWQTQ
                     LGTTTIPVNQTETYGYLTQKTFSVTVTTPGTTYVESRAMDVNGNLSASKVTEVNYDPN
                     AIAAPQITSTPPSITASNAASFSFTGAISGTGYECSLDGGAFSDCSPPKSYSSIADGQ
                     HEFRVRLKSAVASGPAVSYQWQIDSSAPPAPTIHTGPAANTKLTVADFTFSGTEAALE
                     CSLDGATFASCTSPRNVFVPTDGVHRFEVRQKTITGVPGTSAVYTWTVDNTKPPAPVF
                     GEGPSSPTNSTSASIQFTGEPGGTYSCQLTGPGGLQINNCTSPRNLAGLAQGEYTLSV
                     SQKDQLGNQGPAASYSWTVDLTAPVAPQVGSGPPARTASRDATVTFTGEAGGHFECAL
                     DDADFFFCSSPRGLSGLADGEHSLRMRQIDAAGNTGAAASRSWTVDATAPEAPGLDGR
                     PPALTNSAAAAFTFTGEPGGAFECSLDQAPFSGCATPQTLGPLADGSHQFRVRQKDDL
                     DNTGPAASHSWTVDTTPPAAPTIAPQTLELSQNRDRQIGFTGEPGGSFECRINEGVWS
                     NCLPLWDATFPGAGNHSFDVRQTDDAGNTGPSARGQVTIWGGPPSTPVITSAPEGTVR
                     STSAEVRFTAGPVGEPYCNLNGGNWQACDGVWTAQNLEQGPQTLAISQRNEVAGPAVV
                     VQWTVDTLAPKLSGKVTAKPKGKVKKGKPRTWILTTSYDRKLEKPAALEFSLSKAKPK
                     ATIAPVRSRTRKWAPKLTIKSTPKPAWVRVLDPVGNSSPWVRVR"
     gene            complement(32890..34398)
                     /locus_tag="PLJ64_00335"
     CDS             complement(32890..34398)
                     /locus_tag="PLJ64_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC91971.1"
                     /translation="MDRVAESHPRRWAILGVICASLMVVVIDVTVLHVAAPAISEDLD
                     PTALQLLWIIDVYPLVAAPLLIASGVLGDRLGRKRLLVAGMAVFGLASLGATFAPTAE
                     TLIAARALLGVGGAMIMPPTMSIIRDVFRDREERVRAVGIWSAVSAGGAAIGPLIGGF
                     LVEHFWWGAVFLINVPIVLVIIPVAIRLLPESRAAEPPPWDGFAVIMTVIGILGIAFG
                     LKEGARYGFLDPKVLLSLLVGSGSLFWFTLRQLRAPEPLLDVRLFKRREFTAAVGCVF
                     LSMFGLVGIEFFGAQYLQLVLGLEPLEAALRLLPLMLSTLAGGLLAARLLGRLGTRWT
                     ISLGLAGTALGLVPMLALGLTDQYYLLWPSFLVIGFTLEVALVAANDVIISSVPADEA
                     GQAAAIEETAYELGGGFGVAVLGSILAIVYTAKIGPIEGVTGAGIANAEESLARALEI
                     AGKMASRTADPLIEAAKLAFMSGYHTAIAVSIILVGASAGLAAVLLRPRTPQ"
     gene            complement(34445..36154)
                     /locus_tag="PLJ64_00340"
     CDS             complement(34445..36154)
                     /locus_tag="PLJ64_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91972.1"
                     /translation="MTGRRTILFSLIALTMGLGLGAQAQAAKPKADVVFSNGGRILTM
                     KADGTERKVLFGKDRLPRNDGLGAVEPSVSPDGTKITFGFRRLDRYDSLFDVWVMNFD
                     GTGARKLLASNRKQRYADPEFTAGGRIIAASFRENSSLAEARIFTMDTNGKSRKVMLR
                     LKQRQRPWEGWKSLAEPSLSPDGKHLLYLLDPGYDGTSFEEGYGASLRVLNIDTGKSR
                     EIAGNSLGGDFSPGGNRIVFSEVDSEGDNDFCWSYDYSCINFSRIRLVNLNGTGARDL
                     TSLDRRAADERRPDWSAPGRIVFQSTRGPEREVAETTEIWSIRPGGECLARLTNGSPA
                     SLSPVLVDPDGKLTGPRGCNARPPGPNRELTAPDMSGSAIDNLWLGRSHHNVLLSNVE
                     AERNRSTFTYADCGVIPFSGCGRGVLIYNEDICSLQGYTAAYIGSDWAQKQRGIPVLR
                     SLKPGRETPAFTAGFTGRTAFFIIGGSGLGDRKLQHRKEIDALRAVGSPLATGDLPKP
                     RIPVSDIRAMKRVQRVYKRTGSVARTARLLDRGSYFVRSNLKFARIFKAGGYGRINCP
                     GAD"
     gene            complement(36157..37758)
                     /locus_tag="PLJ64_00345"
     CDS             complement(36157..37758)
                     /locus_tag="PLJ64_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91973.1"
                     /translation="MRLRFLAGLLAGMFGCLVLWPGGGQATTPPLAPLAEVVFENGGR
                     IVSIKADGSGRKVLTRKGTDLLRRNYLADIHYFGDSQPAISPDGEKLAFVRYRPAAHS
                     QQVMLADRDGWGATAVKGINAEIQSLDFMPDGRLLLTTWDYRYGYSRTTETFRILALD
                     PATGKKKVLVSKRFFGNYNDDTLQAQEVSPDGKSLLYTRDFGENDDGHDVVQLRIRNL
                     VSGAERVLVTGAEAGSFSPDGERVAFTKMCVDTEGYAHCAHSGDGRTGVWTIGVDGSD
                     RQLIVTGDGGYSSPDWGPGGGYIIFASSRNFPEERQDANEIYSVRSDGECLTWLTNGS
                     PESVMPAWAPETEVRTDPGAKCGAAGRKPLAEARPPRAIDTDPRPRYWAGPIVANRLL
                     SKVDGYAQDEFGSPIYRYGDCPFFDSSDCRRPITIEIAFACGGLGEVRVPPRGREQVI
                     RRRGVRVTRHWTARGKFVTTTLRTGLVDIGIGQSMLDVEDIGGRASTFSDHLAFIDNL
                     RPVGKSLDPGSKLPPAAMVKCQGRR"
     gene            complement(37755..39638)
                     /locus_tag="PLJ64_00350"
     CDS             complement(37755..39638)
                     /locus_tag="PLJ64_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91974.1"
                     /translation="MSTSGGRDNHHNRWPASLIALFAAALLLAAFLLAADRAAAIPPE
                     QLPAATIVFENGGRIVSIKADGTGRKVLTPRRAKTMIETGIRDSDPAVSPDGQTVLFS
                     RDDDSSGSVDRSKLGLFSIDVDGGTPTQIRAMEEIETLQLTTYTGGFAWAPDGSRIYE
                     VEYIVKLVPAGWPYAVRTRIISMLPDGSDLKVIEVGKTTFKNEKPSGDLWIASEVEPS
                     PDGSELLVTRIPMNGNAQPRIDALDLSTRKSKVIEKSAFDPSFSPDGQRIVFSTTRDK
                     VNESCYEGSCSSSQQLYLMDADGSNPRLLLGKIQPWSFAEPDWSGDGSRILLTSNKAS
                     VTTIFGDEIWSVEPDGSCLSRITNGSPASESGSWGLTPDTSPTGCGLGMGSRGVAEVE
                     PPAKIADLEVTPLWLGRNFGLLLFSDYFVDRSNASFTYWDCSWFRNRCPATIAIGSAH
                     ACERNLEGELGNGAYQGMVRRKGGLAVMTGSVRPSLSTIFLADGREVWITSPWRHDGK
                     ATSRAWHMKAIDHLRPYGASKPPASFGAPVFAWHTVHTARVMVRSFRRTQSLKKAAVR
                     AGIFRAGMGISFSYSRRAAQAWLRFSRDLARLGPVKSVECRRWANKPGTIDVRSDFRE
                     LIR"
     gene            complement(39800..39873)
                     /locus_tag="PLJ64_00355"
     tRNA            complement(39800..39873)
                     /locus_tag="PLJ64_00355"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39837..39839),aa:Pro,seq:cgg)
     gene            complement(39925..40644)
                     /locus_tag="PLJ64_00360"
     CDS             complement(39925..40644)
                     /locus_tag="PLJ64_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91975.1"
                     /translation="MSSAFRQRRSGQFRTLIILVVAIGALLIALVGMAIQKPDNEYEN
                     VQGKQDMRQIFGGVRQLDDRLGSDDAPAQMQVFLDAQSSTYKDQFLDTIPALVSGPVR
                     DGTLKLLYRNRSLTRNATERSFYGIEAAMKQGYGWQYAYLMFRNQDLAKEQGIDDDFL
                     TTLAESIDNLEIEQWKQDYQDGLEEGSAMNADLQAQDQIAIDLKIRDAPAAVVNGPSG
                     TEILQDAPDLAEMQAAIGEVQ"
     gene            complement(40646..40909)
                     /locus_tag="PLJ64_00365"
     CDS             complement(40646..40909)
                     /locus_tag="PLJ64_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91976.1"
                     /translation="MAEEPETEVAAASDPFIESLMDTDLYSMGAYFSDRNPELIDELI
                     DRSVAIEERGLVRFSEEEDMELEQAFKTLITGLAVRYYKSVTG"
     gene            40955..41716
                     /locus_tag="PLJ64_00370"
     CDS             40955..41716
                     /locus_tag="PLJ64_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNC91977.1"
                     /translation="MADRNEIIAFCDELLEIESFGDYGPNGLQVPGKAVIGKVATAVS
                     ANLASIEAALATGVDLLLTHHGLFWDFHPRALTGAMARRLRLALGADLNVAGYHLPLD
                     GHPEIGNNALIVRQLGFEPVPGEIGEAKGRHIGQIGRRAEPIPATALFELVGRELGRE
                     PLVFAEGPEMVSTIGVVTGAGASDIHEAIALGLDAYLTGEPSEHVMADAREGAIHFIA
                     AGHYATEICGIAALGDLVAQRFEVEHEFLDLPNPI"
     gene            41778..>43072
                     /locus_tag="PLJ64_00375"
     CDS             41778..>43072
                     /locus_tag="PLJ64_00375"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNC91978.1"
                     /translation="MSSTYTPTNRSRTLADLLPNSAARHGSDGAFLFKNEAGSWEETS
                     YEKVAAEVQALTLGLKALGVQRGDKVAIIGNTRPEWTLFDFAAMSTGATVVPIYQSNS
                     PSECQYVLEHSESRVVIAENEDQLAKIREVRDSLPALENVVIMIGEADDAISMNELRA
                     KGDSGSAEDYKASYEAVEPDDLCKIVYTSGTTGPPKGCMISHGNYRAMLDMADSASIV
                     EEGETSYLFLPLAHVFALLIQYGMIDIGGRIAFWERDQLKIVPNLAEVKPESFPSVPR
                     IFEKVHDTAVATAEGEGGLKAAIFRWAFRVGKRYRKAEAQGKKPGFLLTKQFNFADKA
                     VFSKIREIFGGNLRVAISGAAPIDPEILRFYEAAGVPLFEAWGMTETSTGGTTNMPGE
                     NRIGTVGKPVDGVELRIAEDGELLIKGDNVFQGYYKNPEA"
BASE COUNT         7519 a        14069 c        13940 g         7444 t
ORIGIN      
        1 atcgcgttcg ggaacaggtc gatgaactgg gcgatgaaat agagggcaat gccgaccgcg
       61 gttccggtga aggcacgcat cggttgcgac ttcgcgttga tcgccgcgaa accgaaactg
      121 ccgaccgcga cgaccgcggc gatcagcgac aggatctcga gcgagtcctg gcggatcagg
      181 gtccaggcca tgaagaccgc ggccagcagc gccgcgaccg gagttgcctt cgccgccacc
      241 gccctcgcct tctcccggat gtcaccttcg gtcctgatct caaggaacag ggccccgtgg
      301 gcgaagaaga tcgccaggga agcgaccccg ccgagcaacg tgtatacgga gagcagatcg
      361 aagaaagtgc ccgtgtattc gattgactcc acaccgtggg tcgtcaccgg ttcgatcggc
      421 acgccgccga tgatgttggc ccaggcgacg ccccagagca gggccggcaa caggctgcca
      481 atcgcgatgc accattccca gccggtgcgc caggcgtccg tgtcccgctt gccccacatc
      541 tcgaaaccga cgttgcggac gatcagggcg accagaatcg cgagcaaggc gatgtagaag
      601 ccggagaaaa gggttgcgta ccactccggg aaggcggcga aggtggcccc gccggcgacc
      661 agcagccaga cctcgttgcc gtcccagacc gggccgatcg tatgcaggat cgcccgcttc
      721 tcttcccggc tgccgccgat cagcttcatc agcatgccga tcccgaagtc gaaaccctcg
      781 aggaccaggt acccgatcca gaggaccgag atcaggacga accagacgaa ctgaagtgtt
      841 gttgtgtctt ccatggtttt cctccggctc ccctagtagg ccattcccag gtagggcttg
      901 tcttcctcgc cttcctcaat ctcggccggg cctttgccgg ccgtcttgaa gaagatcttt
      961 ccggcgaaca ccgccagcac gccgtagagc agggtcatcc cgaccagcga gatccagacc
     1021 tcggtagcgc tcaccgtcgg tgaatttgcg tcctgggttc tgagcagtcc gaacaccacc
     1081 cagggttgcc ggcccatctc ggtgaagatc cagccggcgg ccgcggcgat gaacggcagc
     1141 gacgcggccg gcatcgccag cttcaggaaa cgacgcgagt cgggcagctt gcccttgcgg
     1201 ttgaaccacc agccgatcac gccgaacagg gccagcagga agccgcagcc cagcatcacg
     1261 cgccaggacc agtaggccac ccagacgatc ggcgcgtagt ccatcgtctt gaactcggga
     1321 ccgcggtact cggggctggc cggatcaccg aaccgctgcc tgtactcctc gttcaggttg
     1381 ttcacgccct tgacctcgga gtcgggcttt ccggtcatga tgaacgagag ggcgttgggc
     1441 aacttgatgt tgcggttcgt ctcttccggg ttgccctcga acttgccggt ggcaaagagc
     1501 gacaggccga ccccgtcctc ggtctcgaag acggcatcgg cggcggccat cttcatcggc
     1561 tgctgctcgc tcatcaggat gccgttgaag tgcccgacca ccagggtcag caggccggca
     1621 accgcgagcg aaggcagggc gatcttcatc gagctcgaga agacctcgac ctcgttcttg
     1681 cggagcaggt gccaggcgga aatgccggcg atcaccatgc caccggtcag ggccgcggcc
     1741 agcatggtgt gggtcatcgc gtagagcgcg gtgctgttgg tcagcacgtc ccagatcgat
     1801 ttcaactcga ccttgttggt ggcctgattc agctcgtaac ccaccgggtg ctgcatccag
     1861 gagtttgcgg ccaggatgaa gaaagccgag agcatggtcg aaagcgagac cgcccacatg
     1921 caggcgagat ggaccttctc ggagagcctc ccccagccga agatccagag tccgaggaaa
     1981 gtcgactcga cgaagaaggc ggcgaggccc tccatcgcca gtggcgcccc gaagatgtcg
     2041 cccacgaagc gcgagtaggc agaccagttc atgccgaact ggaactcctg cacgatgcct
     2101 gtcgcgacgc caagcgcgaa cgagatcaac atcagcttgc cccagaaacg ggtcgccctc
     2161 agccacttct catccttcgt gcggtaccag cgggtctggt accaggccgt gaaaaaggcc
     2221 agcccgatcg tgagcggcac aaaaatgaag tgatagagcg taactattcc gaattgccag
     2281 cgggccaact cgagactggt cattgcaatg gtgctcattc gtccaccttc atggatccca
     2341 gattagctag gcaggaggtc gaaattcctg cggaaaatca ccgcagttac tgcggggaag
     2401 tacggaggtt tggcttagcc ccggctaagc ccgggggcat cgaccggcat cgtggagcgc
     2461 cttgcggtag cggcgcatcg acgcgtgccg gaagtcgccg tgggtctggt tcttcatcgc
     2521 cggcaccagt tcacggtgga acctcgaacc gagactctcg ttgtgcccga tcacgtcccg
     2581 gatgccgatg ccgtatcggc agcggagcca tttggtcagc cggatcgagg ctctcatctg
     2641 tcgcgggttg ccaagcacgt caccgtcggt gaagccggtg tgctcgatcc cgaaggagac
     2701 gtggttcagg ccgaccgtgt ggcggcagcg gatgccgagc gggaccagct ggaccacgcg
     2761 gccccggccc gagaccacga agtgggcgca gacgttgggc agctcgtgga attcggggtc
     2821 gggccggttc gcggcaaaag tccggtacac cccggcgacc gaattgccca cagcaacgtg
     2881 ttcgacgatc tggcgcggat tccggaggcg ccaggatcgc accccgtagt gacgcagcga
     2941 gtagcgggcc atctggtgct tgcgcttcgc gccgaaggga atgaagtgcc ggtgaatcca
     3001 ggccggcatc ctgagggcgg cccggttcga ctgccgggct gcgcccggtg gcgcggcccc
     3061 ggccgccggg ccggctgcga cgccattggc ggacgcgctc gatccggcgt gttcagcgac
     3121 cgggccggcg agcaccccca gggcagcgac cagcccgacc gcagctgcgg ccgttgccag
     3181 gcgcttcata tctctacccc gggccgggat cagtcgaagg cggccgggtc caggccaccg
     3241 gtcgggccga gatccggacc gagtccgttg cccgaatgct tcaccttgta gccggcaacc
     3301 ttgccacgga aggcgttgcc gaaggtgtag accggcatcc cgatctcggt gatgttgtag
     3361 atcctcggct ggtcggcgat gaaggtcctc agacagccat gactggccgg gtaatccgga
     3421 acgtcggcgt acccgtgggt cgcgtagccc cggatgaagt acgaggagta gtacatgccg
     3481 tgcgagttca tgcccggctc tttccagtag aagctgaagg tgccgaggat ggtcggggtc
     3541 gaggccttgc cgctggcgat ggggaagatc gcgtagggat tgtcaccctt cgaaaagacg
     3601 atcacctgct tcgagagcgg ggcttccatg tggtcgccca gttcgggccg gcggacgtgg
     3661 tagcccccct tgccgttgaa gacacgccgg accacggtct tcgaggcccg cgcgttgcgg
     3721 gtcatgtcgt tgagcttgcg gtaggcgagc acggcgcgtt ccatcttgcc gttgaagcag
     3781 cgaccctggt cgggaaccag cgccaggcgg ttcagggcct tgcggaatcc ggccacgacg
     3841 cggccacact ggtcatgggg caggccgacg taacggaccc gccaggactt gtgcctggtc
     3901 gtgtccttgc cgaccgcacg cttgccggct ttgccccagt acttggcgtg cacgccgtag
     3961 cggttgccgc cgcgcatgta aaggtaggtg cggaaggttc cgtaattgcc gccggccttg
     4021 tggaccttga ccttgcgggt cgagaacttc tcgccgtcct tgtagaggta gaccttgacc
     4081 gtctggaggt tgcggaaggg cttcagcgtg ccctggatca cgacccggct catcacggtc
     4141 gcgcgatggg catgaacgcc cttgagcttg atcttgaccg tcgccctggc cggaccgccg
     4201 gtcggaaccg cgccaccatt ggctgctctg gcggtgtcgc cagccagcat gaaggcggca
     4261 atcgcgcaga tcagggtgag cgctgccgga aggctgaatc gggcgaaggt acggggtatc
     4321 gatcggacgg tcaagtgaag ctcctgcggt tgggcggcca gtccgcaaat tgtagagata
     4381 tggccggatc aggccacggg tgaagccgct tcggcaactt cccctgcctc tctcgtttcc
     4441 tcgtccatcg actcggcctg attgccgatc aggaagaagg cgacgagcac cccggcgatc
     4501 gcgatcccga ccgagagggt catcgcgttg ctcaggccgt agatgaaggc atcgtgaccg
     4561 gcggcgacca gacgctctgc cttggccccg gaaatccctt cgatgtgggg cgcgccaccg
     4621 gtcagctgat gggcgaagtc cgaacgctga ctggcggtca ggccgttctc gccgccgagg
     4681 gtgctcgcca gccgggtctt tgcttcgctc tggaagaggg ctccgagcgc cgccacgccg
     4741 aaagttccgc cgaccatccg gaacatcgaa agcacgccgg aggcgatgcc ggccttggtc
     4801 ttcggcaccg cgttcatggc cgcggtcgac atcggtgaca tggtcaggcc gatgccggcc
     4861 ccgaggatca cgaaactggg caggatgtcg gaaaaggtcg agccgtcgct gatctgcgag
     4921 aagaggtaga gcgaaagcga gatcagggtc atgccgacga cgatcggcca gcgcggcccg
     4981 atccgatcgg ccagccggcc cgcgatcggc gcgatgacca tgatcaccag ggtggttggc
     5041 aggaacctga ccccggcttc gagcgcggtg tagccgagga tgttctgcat gtagagggcg
     5101 aggaagaaga agacccccat catcgcgaag gagatgatga aggcgaccac caccgagccg
     5161 atgaagttcc ggctcttgaa gagcccgaac tcgatgatcg gtgccgtgac ccggttctcg
     5221 gtgaagatga aggtgatcag gccgatgatc gaaaccgcga tcaggctgat cacggcggtc
     5281 gaatcccagc cccaggtgtt gccttcgatc agggcgagga ccagcgaggt gagagcgacg
     5341 gtgaggatgc cgaccccgag gtagtcgacc ttgcggccga ccgtggtgtc gcgcgactcg
     5401 cggaccacga aggtcgcggc gagcacggcg agaaccccga ccggcaagtt gatgtagaag
     5461 atcgcgcgcc aggagacatg ctcgaccagg aagccgccga ggaccgggcc gaccgccagg
     5521 gccagagccg aaaccccggc ccaggtcccg atcgccttgc cgcgctcatg ggccgggaag
     5581 gcgtcggtga tgatcgaaag cgtgccgggc atcatgaaag cggcgccgac gccctgaagc
     5641 acgcggctgg cgaccagggc gaagttgctg ggcgcgaagc cggccgtggc cgaagcgacg
     5701 gtgaagatga ccacgccgaa ggtgaaggcc ttgcggcggc cgaagatgtc accgatccgg
     5761 ccgccgaccg ccagcagcac cgcgaaagag agcgtgtagc cgttgacgat ccattcgagg
     5821 ccggagatcg aggtgtcgag gtcgcgctga atcgagggga gggcgacgtt gacgacggtg
     5881 ttgtcgagca tcaccatgaa cagggccagg cacatcgcgc ccagcgtcca ccacttgcgg
     5941 ttctcgtcgg tgatcaggtg ctggtacttg ctcactgctg aactgctcct tccaactgcc
     6001 ggtgaattga ggcaaggtct tcccgctcga tcagcttcga gagcggggag tcgagggctt
     6061 tgagttcgtc ctcgctgaaa ctgtcggcca gtctcaccgc gccgaccgca cggcccatca
     6121 gggcggcgtt ggtgacccgg tggccttcgt cggtgagcgc gatcagccgg cggcgccggt
     6181 cgtggggatc ctcgacccgg gaaacgaggc ccttgcggac caggctgtcg atcgcccggc
     6241 tcacggcggg catcgaggcc ccggttgact cgccgatgtc agcgaggcag aagctgtcgg
     6301 cgggttcgaa gaccccgaga ctcatgatcg ccttgaactc gagaaatgag agaccgagcg
     6361 cagccgcgtc gagcagggcg ctggtcgccg ccccgtaaga gagcatccgc cggaacaggg
     6421 tcccgagctg cagggcgacc cgcttcgcct cgggcgatgc cgcgtcgatc acttcctgcc
     6481 cggcaacttc ggccgggtcg acgcccggac cccgggccgg atccgtggca ccggcagtcg
     6541 cctgctcgtc gatcttcagc gcttccgcat cggtcatgac tgaagatact tgctctttcg
     6601 caactatttg tcaaacgcaa tagttgctta tgtgatgcac ttcacacggc tggccggggg
     6661 gccggttcgg gggtgccttc gaggttgagc ttcggcagcc actcgagcca ggccggcagg
     6721 taccagttcc acttgccgag gagcttcatc gaggccggaa cgagcacacc ccgcacgatc
     6781 gtcgcgtcga tcaggacggc cgccgccagc ccgaccccca tctgcttgaa ctccagcatc
     6841 gagagggtgg cgaagatcga gaacacggcc accatcacga tcgcggcgga ggtgaccggc
     6901 ccggcagtgc gccggaggcc ggcctcgatt gcgtccgaag tgctcaaccc ctggtcgtgc
     6961 gcttcccgga tccggctgag gatgaacacg tggtagtcca tcgagagtcc gaacaggacc
     7021 acgaagagga acatcggcag ccaggcggtg atcccgccca tcgactcgaa accgagcaga
     7081 ccctcgagat gcccttcctg gaagacccag acgagcaatc cgtaggcggc gccgaccgag
     7141 agcagattga gcaggatcga ggtcgccggg atcacgatcg aacggaacgc gaccatcagc
     7201 aggatgaagg cggtcagcaa gacgaaggcg aagaccagcg gcgcccggct cttcatcagg
     7261 tcgttgaaat ccttcgaacc ggccgtcatg ccattgacgt tggtttcggt gcccggcacc
     7321 ttgccgatcg tctccgggat cacctcctcg cggagcttga ccagcgcgtc attggatgtg
     7381 tcatcggtgc cgctgccggc caggggcacg ttcaccacct cgaccttgcc gttcgggctc
     7441 acggtcgtgg tgaccggtga gccgaactcg gccggaccgt cgaccgcctg cctgagcgcg
     7501 gcgatgccct gctgaacctc gggcgcacgc acgtcatcgg cctcgatcac cacgttggcc
     7561 ggctccgggc cgccggggaa ggcttcgtcc atctggttgt aggccttgat caccggcagg
     7621 tcctgaggca gggcttcggc ccccggcacg acggtgtgga gtttgaaggc cgggaccgcc
     7681 agcaacacga gcaggccggc ggcggcgaca gccgagaggc cggggcgccg cagcacggtc
     7741 cggatgaatc gaccccagaa accgccgccg ttgccggcag ccagcacgct ctcctcgccc
     7801 ccgacccgcc ggatcttccg gctccgtcgc agccggtcga tcagcgggat ccggcccttg
     7861 tcaacccggt cgccgagcca ggcgagcacg gccggcagca cggtcagtga accgagcacg
     7921 gcgaccgcga ccacgatgat cgtgccggtg gcgaatgacg cgaaggtggc gtctccggtc
     7981 atgtacatgc cggccatcgc gatcatcacc gtgattcccg agacgatcac ggcccggccg
     8041 gaagttgccg cggcagcctc cagcgcggcc gattcggacc gtccggccgc ccgttcctcc
     8101 cgctcgcgtc gcaggtagaa gagcgagtag tcgaccccga cggcgaggcc gatcagcagg
     8161 atcaccgagt tcgcggcgtc cgacatcgga gtgacgtggc tgaccagtgc ggtcaggccg
     8221 atcgccgccg cgaccgaagt caggcccagc agcaaaggta cgcccgcggc gatgaacgct
     8281 ccgaaggcga agaccagaat cagcagggtg accggaagcg aggtgaactc ggcctttttg
     8341 aagtcatcct cgaaggcctg tccgatctgc tcgctggccg aggcatcacc gaactgggcg
     8401 aagccgtagc cgggcgcccg ggcccgggcc tcgttgaccg cctcctgggt cgccccgagc
     8461 cgctccgaga gctggtcctc gttgccggcc agctcgaaag agaccagcgc cgattttccg
     8521 tcggccgata tctggccccg gttgccaggt gcaaaggggc tctcgacatg tgacacgtac
     8581 ggcgtcgccc ttagctcgcg ggagaggtcg gcggcagctt cgcgcatcgc ggcgctggtg
     8641 ttcgaaccac cggccggcgc cgtgatcagc accctttcgg tcgcgttctc ggggaagttg
     8701 ttcgaggtcg ccacttcggc gcgccccgac tcgccaactc cgtcttcggc gtcggtcagg
     8761 gtcctcgttc caaccgatcc tccgatgaag acggccagga tgacgaaggc gagccaaccc
     8821 cagatcgcgg tcttgcgatg ggtcgcgctc caccttccgg cacgggctgc gatgtttcga
     8881 ctggtatcca tgccgccaag cctgcctttg cggacgccgg cgcaaaatgg ggcctgcctg
     8941 tgtcttcagc gggtgatgcc tgccccttag gggttcggca aaccgcacca tgaacgcccg
     9001 gatgcgggta ctggccgcac caacggcggt ccccgcatgg gtcagaatca ccccatgggt
     9061 agtgctctga acctgcgggc gaggctgacc tggctggcga tcgccggctt cctggccgga
     9121 gcgggcgggg cgacgatgat cgccctgaca aaccagaccc tgggcgagcc ggtaatcgag
     9181 atcacgctgc gggtgctgac cggatggacc tggatgggag tcggcatctt cgcctggtgg
     9241 cgccgccccg acaatccgtt cggcaagtgg atggtcttca ccagtttcgc ctggctggcc
     9301 caggccctga cctacgcgaa ctcgtcgctg gtcttcaccg tcggcctcgt catcgcctat
     9361 gtctttccct tcgccctgat ccagaccctg gtccgcttcc cgaccggccg gatcgaaacc
     9421 cgggccgacg ggtggatcgt ccgcctggtt gccggcgccc tcctgcttgt tctgctggcg
     9481 accttcttca gctctcccga tcagctcgga tacgacaacg ccccgagcaa cctgctgctg
     9541 ctggccgact cgccgaccac ttcggccgtc ttcttcatca tcgccaacgc ggtggcgatc
     9601 atcctcgtcc tcggtctgat ccacagcttc acgaccaagt attcggccgc ctcgcctccg
     9661 atgcgccgcc tgatcggacc ggtcctctgg acctccacca ccctgctggc cgtgctcggg
     9721 ctttcctccg tcttctcgat cgccaatctg gatacggtcg aagagatcgc cggcatcctc
     9781 ggtgcgatca tcgcgatgct gctgccactc gccttcctgg tcggcctggc ccgcagccgg
     9841 ctggtccgcg gcgaggcgct cggccggatg ctcgaacggg tggcccacga aagcgatccg
     9901 gtgaagatgc aggaagcggt cgccgaagcg ctcggcgacc cctcggtcga gatcgccttc
     9961 tggctgcctg acaccgccgg ctacgtcgac gcccgtggcc tgccgatcgc cgagcctgat
    10021 ccggattcga accgggcggc gagccaggtc acgatggacg gcagaagggt cgcgctgatc
    10081 atccacgatg gcgccctgct cgaggaaccg gagaagatcg aggcggtgag ccgtggcgcc
    10141 gcactggctt tcgagaacgc gcggctggag gccgaggtcc gggccaacgt ggccgaactg
    10201 agggcttccc gcacccgcat cgtcgaggcc ggggacgccg cccgccgcga gattgaacgg
    10261 aacctccacg acggcgccca gcagaacctg gtctccctcg ccctcaaact gcagttggcc
    10321 cgttcccggg tcgagtccga cccccgcggc gcgatcgaga tcatcgacgg cgccagcgcc
    10381 gaactcgaac agaccctggc cgagcttcgc gaactggccc gtggcatcca ccccgccgtg
    10441 ctttccgacc gcggcctgga tgccgccctg ctcgccctcg cgactcgcgc tccgctgccg
    10501 gtcgaggtcg gagaaatgcc ggatgatcgc ctgccggaac cggtcgaggc cgccgcctat
    10561 tacgtggtcg ccgagtcgct gaccaacgtc gcccgctatt ccgaggcaag ccgggccaag
    10621 gtcgacatca cccgggaaaa cggggtggtc cgcatccgga tcgccgacga cggcgtcggc
    10681 ggagccgacc ccgcccgggg gtccgggctg aagggcctgg ccgaccggat cgccgccctg
    10741 gacgggcact tcatgctggc cagcccggac ggtctgggca cgatcgtcga ggcggagatc
    10801 ccgtgcgggt agttgtcgcc gacgactcga tcctgctgcg cgaaggagtg gtccgcgtgc
    10861 tcgaggacga cggtttcgag gtggttggcc aggcgggcga cgccgaagac cttctccgca
    10921 aggtcagtgc ccacaagccc gatgtcgcgg tggtcgacat ccgcatgccg ccgaacctga
    10981 ccgacgacgg gcttcaggcg gccaagaaga tccgggccga acacccggac accggtgtcc
    11041 tcgtgctttc ccagtatctg gaggagagct acgccctcga cctggtcgcc gactcggccg
    11101 aaggggtcgg ttacctgctg aaggaccggg tcgctgaaat cgatcgcttc accgagtcgg
    11161 tccgccgggt cggcagcggc ggctcggccc tcgaccccga ggtggtctcc cacctgctcg
    11221 gccgccgccg gcgcgaagac ccgctcgagg tcctgacggt ccgtgaacgt gaagtcctcg
    11281 ggctgatggc cgagggccgc tcgaacatcg cgatcgccga gaacctggtc gtgaccgaac
    11341 gggcggtcga gaagcacgtg acctcgatct tcagcaagct cgacctcacg cccacggccg
    11401 aggaccaccg acgggtcctt gcggtcctca cctacgtcaa ggcaatcggc gactgaacca
    11461 gaccaccacc accgcgagat ccgacttttt cgccgctatg acgaagaagt cgcggctcgc
    11521 gaatttcact cctcgtcagc gaggacgcgg cggcgggagc gacggaccca ggccccgttg
    11581 agcgccaggg cgatcagcaa cacgatgccg atcggaatcg ccaccgccag cgcgagcacc
    11641 gtcaccccgg ccgcgacacc gagcatcttg cccgcatcgt caatcgcatc accgagaccc
    11701 cagctcgacg aatcgccgcc ggacgagctt tcgccgcccg tctcgacccg caagtcgacc
    11761 ggggtgtagt ccgcccgccg ttcgagccgc ttcagccgtg attcgtagta gctcagctcc
    11821 gagcgttcgt tggctagctt ttgttccagc tcctgccttg tcgcttcatc cggagcgtct
    11881 tcgagctcac tcagcaggct ctcgatcttc gccttcgagt cggctacgtc gtcctcggtt
    11941 cggttggtcg gcgccgtgat gtcggtcagt tcctggctgc gcgacttcag atcggcgatc
    12001 ccggagagat cggccacggc ctcttcgacc tgaccggaag gaatcagcag gctgaagctc
    12061 gcgccggcgt tgccggcctc accgtcggtg accgaggagt ccatgacgat tccgttgtgc
    12121 tcgtcggtca cggagacgac ctcgttggcc acgtcctgaa ccccgtcggg atcggtaccg
    12181 agcacgatct cggcctccaa ggcaacatcg cgttggcgag aaccgccggg cacggtggaa
    12241 ctcctcgagt tctccgatgc ctgatccttc attgccgagg agggaagggc tccgtatacc
    12301 gccccggtcg aggcctcggg cacaggcagg gtggcggagt ccgcggtacc cgccgaaccg
    12361 gctgagatac cccccgattc gctggccgac gatgcggaag tgtccagctg tttcgacgca
    12421 acaacaccgg agtcgccttc acccccgctt ccgccgccaa acctgccgga gtcgacggcg
    12481 ctcaccacga ccgtggcgac cagcaggagt cctgcgaacc cggccatcgc cgggagcagg
    12541 atggggcgcc tcccggtgcg gcgtcggaac cgttccgcga tgctgcctat ccctcccctg
    12601 ccctgtttgc cgagagcggc ttcgtcggcc cattcgggcg ggaaatggtc nnnnnnnnnn
    12661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccctgccctg tttgccgaga gcggcttcgt
    12781 cggcccattc gggcgggaaa tggtccgcga ccgcctggtc gagacgctcg gtgaagccgg
    12841 gctccggggt gggccgggac tcgtggagag cctgggccag gtcggtgaat tcattttcaa
    12901 gttcgtggct catcgggcca cctcctcgtc tgatgcggag ccatctgtga gtgcgctccg
    12961 aagtcgtgtc attgcgcggt tgatgcgggt gcccacattg gtggacgagt agccggtcgc
    13021 ttcggcgatc tcggtgttgg tcaggccggc gaagaacttg agggcgacca tttcgcggtc
    13081 ggccggagcc agggtgccga gcgccgccat aaggtcggcg cgctgttcgc gttcgagcac
    13141 ggcgccggtc accgagcccg gggcctccag cccgccaggc tcgatcgccg gcacctcccg
    13201 cttgcgtttg cgaagttcat ccagggccgc gttccgggcg atcccgaaga gccagccccg
    13261 ggcgctcccc cgcttcgggt cgtagcggtc ccacttgcga aaggcccgtt cgaaggcgag
    13321 cgcggtcacg tcctccgccg ccacccgatc ccgcagcaga ccagccacat acgagtagag
    13381 gtcgtcgcga ctgccgcggt aaaggtcctc gaagagaggt cgcggcccgc tcttcggacc
    13441 cggggacgaa acaggttcag ggacagcttc caacatgccg ttcatatcca gactacgggc
    13501 ggcatcgcga tccgtcacat ccccagcctg ccgagagtcc gcccgcatgc ttcgtgggtc
    13561 ggatttccgg ccctgctgcc ggttatccga cccactgccg gtgataagcg gcattgacgg
    13621 gggaatgtgg ggtcacccgt agacccgtgg cggctccgag gccggttcag gccccggatt
    13681 cgagaggtgc cgcacattca cgccctttac gggcctgttt gtgcggcacc tcccccggat
    13741 cagggtcggt gaaccgtcga gtgaccgacg aaggtcagcg tgcagctgct ggatcccggg
    13801 tgcgttgccc gggatccggg gttatcagcc ctcgtagggt tcgaagtcag ccttgcgggc
    13861 gccgcagacg gggcagaacc agtcttcggg gatgtcctcg aaggcggttc ccggggggat
    13921 gcctccgtcg gggtcgccct cggcggggtc gtagatgaat ccgcaggatt cgcagatcca
    13981 gagttgggtc gtagcggtgc tcatggcggc cagattaccc gagagcaggc aggcaaaacc
    14041 ggccggccag gtcgcctctt gtgcgcgcac gcggcgttgt cgccgttctg gcgatacggt
    14101 tcgtgaggtg aacaagtttc cggaagccgg accaggggag tcgatcaatc ttggcaagcc
    14161 gtcggagccg cgacccgctg ccaccatttt gttgctgcgt gaccgggagc agctcggcac
    14221 tcccgaaaag ggcgtactcg aggtcctgat gctgaagcgt tcggctgaat cgcacttcat
    14281 gccgagcgtc tgggtttttc cgggcgggtc gctggatccg gaggacggtg acggactcga
    14341 cgggcttcgc acctgcgccg cccgggagct cgacgaagag gccggcatca agctcgatct
    14401 gtccagcgag ttgatccccc tcgcccgctg ggtcaccccg gaagtggtca agacccgctt
    14461 cgacacctgg ttcttcctcg cccgcgcccc cgagggcgtc gaggtcaaac ccgacaattt
    14521 cgagatgacc gaggcactct gggtcaatcc ttccgatgct ctcgcggcag ccgaggccgg
    14581 ggacatggcc atcgtgtttc ccactctgaa acagctcgaa gcgcttgcgc cctcggcgaa
    14641 cacggaggag gcgatgggcc gcgctgccgc cgaccccaac gcctcgaagg tcgtcctgcc
    14701 gaaggtgatc ggggacgagc agaacccccg ggtcgtcctc ccgaacgaag acgattaccc
    14761 ggactgactc cggtcagtca gccggaaaag gctcaaggcc ggcgttccat ggtccgccgg
    14821 gataggtgtg gatcccgtcc tcgtccatct cgctgtgaag ccgtttgaag agctcgccgg
    14881 cgttggcaag ggcgagtttc agctcctccg cagagcgttc cctgagtcgc cagagaaacc
    14941 agagctgacg gtcgtttatg ccgaagtcga cttcccggtc gatcgtggtg gtccactcga
    15001 gaaagccggg cgagaacagc tcccgcagcg cgatcgggtc gtgttccttc ggcaccgtcg
    15061 ccagaaagcg cttgttgaag tcgaccgatt cgaattcgac cttgcggcgg gagaccttct
    15121 gcttgactag ctcgctggtt tcgatcgact cgacgttgaa ggccgggacc acctgggcca
    15181 ggtcaggcat gtgggccttc gcgaggatcg cggtcgggac cagttccttg cgcatgaatc
    15241 cgccgacttc ctcctcgtcg gcgtcgcagc ttgctcccca gaagtccggg gcgagctcgc
    15301 cggtcagccg gttgctgccg ccgacacaaa gcgcgaccgg tgcgccgacc gggtttatgc
    15361 cgccaaccgg ctcccatttc acctcgcccg cccccttgta ggaagagacg atctgctgcc
    15421 actggtcagc ttgcttgccc atcgccgatc accctacccg tagtcacagc cgaatcaggc
    15481 ggataggatg gccgcgatac tcatttcaac cgaggagact tgcctatgag tggcgcactt
    15541 atcgcgctga tagtggtcgt cgtaattgcc ctgatcgcgg tgctctggta catcgcgacg
    15601 cggaacggaa tcatcgcttc gcgcaaccgg gtcgaggaat cgtggagcgg catcgacgtc
    15661 cagctgaagc gccgacatga cctggtcccg aacctggtcg agacggtcaa gggctacgcg
    15721 actcacgagc agaaggtctt cgaggaagtc accaaggccc gtgccgatgc gatgaaggcc
    15781 cagtccgtcg acgagaccgc tcaggcggaa tccgggctca accgctcgct cgccgacctg
    15841 cgtgccgtgg ccgagcagta cccggacctg aaggcgaccg agaacttcca gcagctcagc
    15901 cggaacctct cggaactcga ggacgagatc caggcttccc gccggatcta caactcgaac
    15961 gtccaggcgt acaacacgaa gatccagcag ttccccgcct ccgtcgtcgc caatcagggc
    16021 ggcttcacgg ccaaggagtt cttcgagatc gaggacgccg ccgagcgcgc cacccccaac
    16081 gtcagcttct gagggggcac ggcatggggg accccgaaaa gtaacgagcg ctccgcgctc
    16141 tgattaagga gtcacttttc ggggtccccc atcccgcgcc cttccggacc gggctgggac
    16201 ccctgcgggc caccttcgcc ctccgggggt gaggagggta aagggggacc gaagagagtg
    16261 gcccaccaga ctcgggtcag gctcgaggcg acctcgttcg ggtcgtcctc gccttgggca
    16321 aggtggacct ggaagctcct ttcggtcatc agcaccaggc cttcggcaag tgcttccggg
    16381 tccagtgatt cgttgaccga gcctgccttc tgcaaccgct cgatctcctc ccgggtgcct
    16441 tcggtggtcg tttccagcac ggtcgaccag agctcgcgca cctcttcgtc gtaggtggct
    16501 gcctcgccgg ccagggcgag cagggccgag ttgccggcgt agatccgggc cattccgtag
    16561 atgccccgtt cgatgtcgcg gcgggggtca tcaccctgct gccagcagcg gccgagccgg
    16621 ttctggtggg aggcgatcag atcctccagc gcccccagca gcagctcttc cttgtcgccg
    16681 aaatagacgt agaacgccga gcgggcgacc ccggcctcgc gggttacccc ttcgacggtc
    16741 aggtccttga acgaaccggc cgccgactgg ttcagggcag cctcgatcag ctcctgccgg
    16801 gtccgctcat gacggcgtcg tcggttatgt cggcgctcca tcgaggtcgg cacccgccaa
    16861 gcatatgcac cacccccgcc ggaaacccga ccggcacgaa tcgctcagga agagggcgcg
    16921 gccgccttga tccggcggaa ggcggaattg gggctgaacc agggcccgcc ttccggggtc
    16981 gggaacccgt tccagaccgg attgaactcg ccgcgcaacc cgaagacccg cttgtggagc
    17041 cggcccggcc tggtgatcgg ggccagagcg ccgcggttga cagtgatcct gaaagtcagc
    17101 ctcgaatcct ttgcgtctga ctcacccgca gtctcgtagg ggttgtattc gggcttggcc
    17161 aggatcgcta tccgtttgcc ctggaccttg accgtgcgcg gggtggcggg acttaccgat
    17221 acgcggacgc gacccttcaa cccgcccgga cgcagccgca gcttccgcgg cagatcgacg
    17281 gtgagcctct gccgggtacc gaccaatcct tccggcgggt cgccgaggct tgccttgagt
    17341 accagcgacg gatgcttgct tccggctccg gcgaacctga ccgaaccgtt gatcccgggc
    17401 ggtcgcaccc gacagatgtt ccagtcaccc gcctttccgg cctgcccccg gctgcatccg
    17461 ttcggcggaa tcaggcgttc accgccggaa ccgaagctct cgacgagatc gccggtcccc
    17521 gggtcgatct tggccagggc caggtgattc cggaagacac aggaatcgga gcagtccggg
    17581 ccttgcgcgt tgccagacac cagcagcgat ccgtcgctca gcgcggtgac cccgtgcgca
    17641 agcgaatcac cgggcgaaaa ggtcacggaa cggatgcccg gccagttggt tgttccgacc
    17701 tcgaactgcc agtgctggaa cccgcgcccg gggaaacgca cgaacgcgac accaccgtca
    17761 aacggcgcga ccgctgacac atggtcgccg agcgccacca atgcatcttc agcggctgga
    17821 gatccgtcgc tgtccagcga aacggcgtag gcggggtagt aatacatgcc cggtgtccgg
    17881 tcgaggtagg cggtgatcga gtcatccgga tggacggtca ggccgaagac ctcgccgctg
    17941 ctgtacggga gcccggcggg gaggtccgcg tatccaacat tgtcgggacc gaacgaaagg
    18001 tcgaggctcc cgtcggcaag caggcgggtc gccccgtcct ccccgaccat gaccacgcga
    18061 ttcgagttgt caaccgcgat cgccgcgtca aaattcagat cgagttcagc gctcacgcca
    18121 gagttcccgt agccagagac aagttgaccg gtttggtcgt acttgccgac caccggcgtg
    18181 gctgttgaag gacgcctgcc cgcaaccagc aggttcccgc cggagtccaa cgcgaaatcg
    18241 aaggcggagg cgaggttcat ctggtcggcg gcgctgaaat tcaggactcc gtggttaccg
    18301 aagctctcga ccgggtcacc gtccccgtca aatgaccaga tccgtttctg gctgagaacc
    18361 agaaaacttc cgtccggacg gccaatgatc ttcaggccga attcgttctc ctcgacggag
    18421 atcaatgcag ccccgccgtc ggcaaaagcc gtgcggtccg acccgtccgg attgagcccg
    18481 accagaacga tccggggcgc aggtgaaacc ccgtagcggc agtcccccat gcccacgatc
    18541 gaaccgtccg gctgctcgat cgtctgcttg atttccgagc aggaggaaag ttcttcgttc
    18601 gtcgcagccc gcgccggagg tccggccagg accgccacgg cgaagaacgc gaccagcgcc
    18661 gccaatcccc ccaaggatga agttttcagt cccggcgttc ctgccatttt cccgactctc
    18721 cttgacttga gcgtatcgcc tcagccgccc ttgccgggcg aaatcgtgaa ccggagcggg
    18781 atgtctccgg ttgagttctg gacgcggtcc tcggcgcgga accaggcgag gtagcggccc
    18841 ggggcaggtt tgaaattcgc ggtgggtctg gcgaaaccga cccggttgat tccgacgtgt
    18901 gcgggccact cgccgtagcc ccggtacacc ctcacgaggc gcttcctgct gcccttgccg
    18961 cgggtgacca gctggtaata gtcggccgag gcggtggtcg cctcgttcac cgagaaacgg
    19021 aaaccgatgc cgcggcccct cggaccgacc accggcgggg ccgggctctc gccgtcggcg
    19081 gtgaagaagt agtcatcgac cacgggtgag tcaaagtcga agttgatctt cggcctcggt
    19141 ccccggctgg aatccgggtt gcccggcagg ttcgagacag tgacctcgcc cctgaccacc
    19201 gaatggagtt tgcaggcgaa caggtaggtg ccgggatggt cgaaggtgat cgtgtactcg
    19261 tccccgggcc agtggttcgg ctcgttcctg ccggcgtccg aatcccactg gctggcgtcg
    19321 gtggtggtga tcgggtcgaa ggccgaatcc ctgggcgcct ggccggtgac cgaatgcggg
    19381 gtgtcgggcc cggcccagag ccaggtcacc gattcgccga ggtcgatgtg cgggttcttg
    19441 tccgaccact gaaagtcgga gatcgaaacc caggtgttgt tcgccgtggc cggggcggca
    19501 aacaggaagg cagccgaagc cgccgcgacc gcgaccagcg cgacgcgaag tttcctggcg
    19561 gaacctccgc gcactcctag cgcttcttct tcgggaagac ggtgaagttc agggtgatgt
    19621 cgtcagagga gttcgacttg ccgtcttcgg cccgaaaata ggcgacgtac ttgcccggca
    19681 ccggtttgaa ggtgctcgag cgggcggcga acctgacgtt gttcatgccg atgtgggtcg
    19741 gccattcgtt gtagccctgg aagaacttga cgtacctggc cttcttgccc ttgccgcgct
    19801 tgaccaaccg gtagtagtcg gctgaagcgg agccaccctc gttggtgccg aaggagaatc
    19861 cgatgccctt gcccttgtga ccgatgaacg gcatggtcgg gtcggtgcca tttgaggtga
    19921 agaaataggt gtcgatcagc ggaccctcgg tgtcgaaatt gatcaccggg gcgggcccga
    19981 agtccgaatc cggattgccc tgcgtgtcgg agaccgtgac cacaccgcgg accgaggcgt
    20041 gaaccttgca gacgaagaga tacttgcccg ggtgatcgaa agtgaccttg tactcgctgc
    20101 ccggatagtg gatcgggtag ttcgtgtcgg gatccgaatc ccactgggag gcatcgtcgg
    20161 tcgccacggt gccgatgccg ggcagcccgc tttcggcgcc ggaatttccg gcgggctgac
    20221 cggtgaccga atgctgggtg tcggggccgg tccaggtcca gagcacggac tcgccgagat
    20281 cgacctcggg gttcttgttc gaccagtgga agtcggcgat cgtgacccgg gtgttgttcg
    20341 cggacgccgg ggcggtaaaa gcggaagcga cgaccgcggc cgcaaatacg gcgatgagcg
    20401 cccggagcgc ccggccggcg cctgatgtca gtcgacgatg taccaaccgc tcattcccgt
    20461 cacgagatgg ctgaacacat ggcagtggta gagccagcgc ccggggtttt gctcgatcca
    20521 ccggacccgg aaggagtcac ccgggccgaa gatcttgttg tccgcgtagg tgccacccgc
    20581 ttcctgccag cggtgaccgt ggacgtggaa agtgtgcatg tcattgtcga cgccgtaaac
    20641 atgcatcgcg acgtcgtcac caaccgtggc ccgcatggtc ggcgtgttgc cggcgtaggc
    20701 acggccgttg atcgcgaaaa gcggggcctt cagcgggaac aggccgggca ggtacgaatg
    20761 aaaaccgagg aagtactcgc ggtccggcag gggctcgtcc gggtcgcgga cgatcagcgg
    20821 cccgaaaagg cccttgaaga ccgcggtcga atcgaaaggt ccgtggtcgt ggtagagcca
    20881 gctgccctgg gtgccttcgg ggcattccca gacgtactgg aaggtgcggc ccttctggac
    20941 gaatccgccc gggtcggtcc agtagccctt gtaggcgccg tccatctcgt tcgagtagaa
    21001 gacgccgtgc gggtgcatgg tgaccgggac gtcgcaggcg ttgcggaagt tgacgaccag
    21061 ggtgtcaccg accgtgacgt caagcagcgg gcccggcacg gtggccggac ccagcggctt
    21121 gctgaagtcg gtgtcgtaaa gacggtaggc ataggccggg accttggtcc gcccgaccgt
    21181 cttcttgatc gacgtaccca tcatctggtc ccgttgcttg gggaccacgt tccaggtgac
    21241 gggttcggcc tggatccagt actccttgcg gttgccggag atgtcggcgt tgcttacgga
    21301 attgctccgt ttcgcagccg caaccttcgg cggtacctcg atcttgttcg ccaaagcggc
    21361 gaggtcggtg ttgctgtcaa tcgagtactg cttgcccgcg atggtgcaga gaaacgcacc
    21421 accggccagt ccgaggaaat tcctgcggtc cattggatcc agtcctttct ggctcttgcc
    21481 agacctgatg ctcgcccggt tacttgaccg tgaccgtcat cttcatcgtc gccgagtgga
    21541 gctcgcagac gaagtgatag acgcccggct tcttcagctg cttggggccg aaggtgaaga
    21601 aggttgccgg cgcggttgga gtgttcagcg agtacttctc gctggcggtc agcccgaccg
    21661 ggaacggatc atccagggaa acggtgtgac cgttcgcgtt cgagttgctc cacacccact
    21721 tgacggcctc gccccggttg atggtcacat cggacgggta gtagtaatcg tcgaggacgt
    21781 cgatcgtggc ggcaaccttg accggaggct tggcgagctt cgcgtacttc ttgttgaccc
    21841 gcttgatgca ggccttgcgc ttcgccttcg ccttgatcct gtagcacttc ttcagttcgg
    21901 ccctcttggc cttcttctgc ttcgcggtca ggctggagct ggacgcggct ccgtcacccg
    21961 acgctgaagc agtggcggga accgcgctgg cggcccccag cgccagcgcg acgattccca
    22021 cggacagcaa ccctgctgct cttgacttga tcttgctgct cattttgatt cttacctctt
    22081 gaattgtggt ttggctgggg gcagaactgc tcctggtcag gagcaggtca ccgggtcggc
    22141 ccgatcctcg gtgtacgaga cacccttggc atcggtgtac gccggatgcg gggtgctgac
    22201 gttcatctcg cactcctgct tctgcggcgg gtgctgcgac acgtatccga agatcgtgtc
    22261 accgtcaccg ggaccgttcg acgggtcacc gtcgtcggcg taggaggcga tgttgatctg
    22321 gaggccggcc gtcagttcga tcggcgaaac gtccttctga ccaaccaccg gctgcgggca
    22381 gctgctcggg tagatcgtgg aaagcgcgac cgtaccgttg ccgggggcga agctggaacc
    22441 ggcagtgttg ccctgccagc agttgccacc gcccgagtaa tccgagaaga agtcgaactt
    22501 cccgttcgga tcatcgccgt tgcggcccat gttgttgtcg atgaacttgt tgttcaggtt
    22561 catcgcgtcg tcgccgacgt tgacaccgaa cagttccggt ccggagaagg tggctgcgcc
    22621 ccacttctcg ttgccgaaga tgttgttgtc gtagacctcg acgctgtcgg tgccgtacag
    22681 gacgacgccg atgccgatcg ggtagttgac gtcaccgatc gtggcaaccg tctggaagtc
    22741 agacccggtc gagtagtagt tgtagttgtt ccagaaaatg tcgttgttgt ggatcttcag
    22801 ccagccgctc ggctcgaact gctctgagtc gagggtgttc ggcacgatgc cggcaccgtt
    22861 gttgtagaac gccgagttct tgatgtcgac gtacttggag ttggtgcccg agtagccgag
    22921 gacgttggcg tagctcttga cattccggat gatggtccag tccggatcgg cctggcaggg
    22981 cgtgcgctct gcggcaacgg tccggttggt ccagtcgggc gaatcacacg gggtctcacc
    23041 gatgtagacg gcggagtcac cgtggtggta gcccgaggag ttctcgatca caccaccgaa
    23101 gcagttccgg gcgaagaagc cgtaggaacg ggtgtcggag gagacgaggt tgtccatggt
    23161 gtagtcggca caacgctcgg tgccgtcgtg accggcccag acgaagaacg tgttgttctg
    23221 gtagtggcgg gcccacatgt tcttcatgac gaggccgatg atgctgcggc cttcgatcgc
    23281 gttgttggcg acctggggaa caccgtcgac gatcgtcttg gcgttctcgc cctcgaggat
    23341 gacgtccttc gcattcttcg aggtgcccat gatggtcagg ccgtcgtagt cgtacttggg
    23401 gttacgacca tcgaggaaga cgccctcaac gtaggtgccg ggcttgactc gcacggtcgc
    23461 cttgcgcttg gacttcttct gccacaggcc ggccgcgtca gcgcccttct ggacggtgcg
    23521 gtacttgcag ccgtgcttgc agacgtcgaa gtactgggcg gcgttgtagg ccttcagctt
    23581 cttctggaga cgagtcacgt tctgcttggc ggacttgcag cgcttcttgg ccttggcctt
    23641 cgacttgccc ttctttgcct tcttgacctt cttgcaggta gccgtggcct tcttctgcgc
    23701 ggccttgacc tgcttctgca gcttcacagc accggcgtca gccgccgaag ctggagcggt
    23761 gacggcgaaa gcgcccacca ggaacgcggc aagaatgccg acgattacag ccaaacgttg
    23821 cttcatctct ctccttgaag tgtgaaaagt gtgtgcggct cgaccgcagg aatgcggaaa
    23881 agccatccgg acactctaaa ccacccttaa cggaaaggtt gcggtcctgc gcttaacccg
    23941 gatcggatga attggcgctc tgacgcgatt tccgcttcct gtccgactcc aggggccgag
    24001 caatccgcac tgccatgcga gaaattgaca aggcttcatg tcggttacac ttgattgcca
    24061 tggcgcacga ctacgactac gtgatcgttg gttcgggatt cggcggcagt gtttccgccc
    24121 tgagactggc cgaaaagggc tacaaggtgg ccgtgatcga gaccggccgc cgcttcgaag
    24181 acaaggactt cgcgaagacg gcctggaaca tgcggcgtta cttctggctg ccgaagttcg
    24241 gcctgcgcgg gatcctgagg atgtcgatgt tcaaggacgt cttcgtcgtt tccggttgcg
    24301 gcgtgggagg cggcagcctc ggttacgcca acaccctcta ccggccccgg cctgccttct
    24361 acaccgatcc ccagtgggat ggcctggctg actgggaagc cgagctcggc ccccattacg
    24421 tgaccgccga gcggatgctg ggcgtgacgc tttaccgcga caccggtccg gccgacgcgc
    24481 tgcttcacga atacgccgac gagatcggcg ccgacaagac ctactcgaac acccaggtcg
    24541 gggtcttctt cggtgaagcc gagaagaccg tccccgatcc ctacttcggc ggcgagggcc
    24601 cggagcgcac cggctgcagc aagtgcggtt catgcatggt cggttgccgg cacggagcaa
    24661 agaacaccct ggtcaagaac tacctcttct tcgccgagaa gctgggggtc aaggtgttcc
    24721 ccggcaccga ggtcacttcg gtcaggccga tcggcgggcc cgacggcgag catcgggacg
    24781 gctccgcggg ctacgagctg agaaccgacc gctctggtgc cgtgctgcgg catcggcgcc
    24841 ggaaactgac ggccgccaag gtgatctttt cggccggagc cctcggcacc aacaaactgc
    24901 tcgccaactg cctccataac ggcgacctgc cgaacctctc gaagagggtc ggccatgtgg
    24961 ttcggaccaa ctcggagtcg atccaggccg tgaccgcgca cgatgacgaa agagacttca
    25021 gtgattcggt ggcaatcacc tccagcatct accccgaccc cgacactcac atcgaggtcg
    25081 tcacctacgg ccaggccggg gatgtgatga gccgcctcta cacgatctac accggcaacg
    25141 ggacccggtt gacccggccg atcaggttcc tggccacgat ggcacgccac ccgatcgaca
    25201 cccttcacct gctgttcatg ccccggcact ggtcaaggcg aaccgtgatc ctcctggtca
    25261 tgcaggcgct cgactcggcg atgcggctca agccggtccc gaagaagttc ggcaagggag
    25321 tccggctcca gaccgagcag gacccggaac ggcccaaccc gaccttcatc caggccgccg
    25381 aggacgcgac caagtggttc gcgaagcgaa ccggcggcat cccccaggtt tcctttggcg
    25441 agtcgctctt caacatcccg accaccgccc acatcctcgg cggtgccgtg gtcggccaca
    25501 gccccgaaga cggggtgatc gacaaggaca accacgtctt cggctacgag aacctgatgg
    25561 tctgtgacgg ctcggccgtg ccggccaatc cgggagtcaa cccgagcctg acgatcaccg
    25621 cgatgaccga acgggcgatg agcaagatcc cgcctaagga tcccgccgcc gaactgcttc
    25681 acctgccgga cgaggcccgc ccggcccgga ccgccgacac agtcggcccg gcccgcgggc
    25741 ccggcccctc cgggtcggcc gaatcctgga ctcccctcga gccctagacc gcccgcgcgg
    25801 acagttccgc gagtgcctag aggccgttct tcttcttgag ctgctcgacg tccgacttca
    25861 gctcgtcgac gccaaccgcg ccttcgatcc gcttttcgat cgtcccgtcc ttgttgatca
    25921 ggaagatcca cggctcggtc tggaggccga acgcggtcag ctgcgggcgg atgcccttgc
    25981 tcggatcgtt ctcgttgtag acctcgttgt ggatgaagac gaccccgtcg ccgacttcat
    26041 ccttgacctg ctcggccacg tccacgaccg gtccgcagac ccgggactgg cagagcgccg
    26101 gggtggcgaa gatcagcacc accggtttct tgccgaccac gtccttgaaa tcgtcgtcat
    26161 gcatgctcga gggcggctgg cgcgtatcga tcttcgagag gtcgccaccg acgtcttcag
    26221 cggtcggggt gctgaccttc ggcgcctgct ggccgacctg ggccacgtcc gggtactgcc
    26281 cgacgaccgg actcggtgtg acgcgggccg cttcgaaacc gtcggcggtc ttgaggatcg
    26341 cgatcgccag ccaggggccg ttgcggtcga acttcacatg cgagaggtag aagctgttgg
    26401 cctcgccagg gccgccggca cccttcgagc ggtaggcagg cttggtttcc agtgaaacga
    26461 cctcggccgg catcgggcct tccaccggcg cctgcggaga cttggcgaca tagatcgcca
    26521 cgtcggcatc ctggacctgg ctccggtcca cgttgaagac accgaacgga taccggttct
    26581 cgcccggttc gaacacgacc gcggccggag agacgacctt gtcgctgggg gtctggtcat
    26641 tcagcagctc ttcgatcgtc tttccttcgg ccgaaggaaa gtcggaagcc gaaggagcgc
    26701 cgcgggcaac ctcgtctccg gattcactgc tgccgcagcc ggagatcaac atgccgaggc
    26761 cggcgaaggc aagcagcgcg aggaagaaga gctgcttcgg ggggatgggc aggacagcgg
    26821 tccgggtggg aaggccgatc atcggaagga tcataagtag tcggcgagct tcgccccgct
    26881 tgggcgggtg ggaagaacgt gaacccaccc acagaatcac aaaaagaaag cggcccggac
    26941 taagccgggc cgccagtgaa acccgccggc ccacgacatc caggagtgga tgggccggct
    27001 ctggttggga taactaggag gcgaagcgct ccgctacgta gtcccagttg actacgttcc
    27061 agaaggcgtc caggtaatcc gggcgcttgt tctggtactt gaggtagtag gcgtgctccc
    27121 agacgtcggc gccgagcagc ggggtcttgc cgtcggtcag cggagaatcc tggttggcgg
    27181 tcgagacgat ggcgacgccg tcgccgtcct tgaccagcca gacccagccg gagccgaact
    27241 ggccgatgcc gccggccttg aactgctcct tgaaatcgtc aaaggaaccg aaggcggagt
    27301 cgatcgccgc ggcaaggtcg ccgctcgggg cgccgccgcc gtcgggcgag agcatctccc
    27361 agaagagcga gtggttgtag tgaccaccgg cgttgttgcg gaccgggccc tgcttgtcgg
    27421 ccggcagcga ggaaagattg gcgatgactt cctcgaccgg cttgtcggcc cattcggtgc
    27481 cttcgagggc ggcgttggcc ttgtcgacat acgcctgatg gtgcttgtcg tgatgcacct
    27541 tcatggtcgc ctcgtcgatg tgcggctcga gggcgtcgta tgcgtaggga agatcgggaa
    27601 cttcgtatgc catcagggga ctccttgact ggtttcttga ggtttcgtga caggtaccag
    27661 aaccctaacg gtccgacgcg ctccgcctca cggcaggttg ctgaatcccc ggcagcgggt
    27721 tgtgccgtat ttgccgatcc ggtcgaggtc tcgggcgaag cggaggtagc cccgggcctc
    27781 ccgcgggctg attcccagcc ttctcagcga ggcgaatgaa ccctcctgcc gtttcgtgcg
    27841 gaggaccagg tgcgccgttc ggacgtcgcc gagggtgaag acgggtccct ccaggcggtg
    27901 gagccgcggg ccgctgaccg gctggatcaa ccttgccgcc tcgacgaaat cggcgaagga
    27961 cttcgcgtga ttgatctgga tgtcaaccga aagcccgccg gtgtagacgg ttgcctcgtt
    28021 gcggtagtcc ccgttctgcc tgacgatcac cgttccgcgg acccggcgca tcccggcgta
    28081 gttctcgggg aagaacggat agctgcagac cgcgtcggtg ctgacttcgc ggtagccggc
    28141 acattgtgtg tagtcgaact cggtgcagtc cgagtaaccg aaggagacgc cgttcatgat
    28201 cctgatcagg tcgtcgtaga gcatcccttc ccattccggg cccatccaga accggggcgg
    28261 gtccagcccg cgcacccgtt tggggatgct cgtttcgacg atcgcattcg gggcccggtc
    28321 cccgcagccg ccaggttcga aggagtcccc tgcggtgccg agccagaccg gcgcggtgct
    28381 ctccggcgca ccgttggtca gccgggtcag gcagctgccg tccggttcga ccgcccagac
    28441 ctcctccccg tagctggcga cccagccatc tcgcacgctt tgaaagatga tccggctgcc
    28501 gtcggccgac cagtcgggcc cgcgatcgat gaagcccggc accttcgggg cgatccgcgt
    28561 ctcgccggtg ccgtcagcgt tcatcacact gagcgcgcca tagccctgct tcatattgat
    28621 gaaggcgatc cggctgccgt cgggcgaata cgagccccag gcggctccgg ccttgagcgg
    28681 cgtcacctcg ccggtgtccg gatcaagcag gtcgaggtgg ctgtcgctgg tgtcgtaatc
    28741 gaaggtgagg agcaaccggc cgtccggggc gggcgtgatt ccgccgatcg agtactcgtc
    28801 gtcatcaaga tcgaccgggg gcgcggacca gatggtttcc aggccggttc cgtcgggacg
    28861 gatcgagttg acgctcaggg tgtagctctc ccagtccccg gcgtacctgg tcttcgtgaa
    28921 gtagatcagc gaaccgtccg ccgaaaacgt tgcggcctga atccagctca gggagccctg
    28981 tttgaccagc agcttcggct tgccgccgtc aaccatcagt gacatcaggc cctgcgggcc
    29041 ggttggcctg tcagaccagt cttcccgccg gaagagcacc gacttgccgt cgggcgaccc
    29101 ggccagggcg gtgtcgtgaa gcccgtcgat gctgctgacg aaggagttct tcagggtcag
    29161 gacttcgcgg tcggtggcgt cggccttgat ccggatcagc cgcttgccgt tcgagaagac
    29221 caccgacgcc ttgtcgaccg gcggtggggt cagcgccagg gcaggcgagc tgaccgccat
    29281 cactccgaaa accagcgacg ccgcgacgac gattcccctg agaaatcctc tctccattgc
    29341 gatcctcccg attgcggttg aactcccacc cgaaaactac cttttgtccg ggcacgctcg
    29401 caagccgtac cgggttggac aggggccgct ccggcaggta ttttccttca cagtaagtgc
    29461 acaggggtcg aggccctggg ggaagtggag cgaggagagg gagacttgac cagcggggtc
    29521 ccagtcatca gtcgcgtatt gattcttgcc gcgacgcttg ccgtgtttgc aaactttgcc
    29581 gccggctacg cctctgccgc gcctcctacc ccgaccgccg ccgatccggg catgccgaac
    29641 tcaatcgcgg tgctggggga ttcgatgtcg gtggcgaccg gcatcaacgt gatcccttcg
    29701 gtcacccagc cggagagttc ctggtcaacc gggaccaact cgtcggtcaa cagcctctac
    29761 cagcgcatcc tcgccctgaa cccggcgatc tccggaaaga accggaacat ggccgccaac
    29821 ggcgaggaca tgaccgacgc tccggcccag gcaaacgcga ctccggcaga caccgagctg
    29881 gtgaccatgc agctcggcgg caacaatctc tgcaaggact cggtcgccca gatgacctcg
    29941 gtcgccgagt accgggcccg gttcgtcgag accctggaca tccttcagag ccgcatgccg
    30001 aacgcgctga tccagatcga gagcgtgcct gacatctaca acctctggtt cctgcgcggg
    30061 gcgccgaacc cgcccaatga ccaaccctcc agccgggccg gcacggcgag ggtcttctgg
    30121 gacacgcttg cggtgatccc ctgcaagtcg ctggttctcg acccgaccga tatgagccag
    30181 gcggcgatcg accggcgcaa cgcggtccgc cagcgggacc tcgactacaa caaggccctg
    30241 gcggaggaat gcgaagcccg cctgcgctgc cgcttcgacc gttacgcgac cttcgccttc
    30301 tcatccaacc gggtcgtccc cggggacctc accggtccct acctgcctcg cgcccagtgg
    30361 cagttcgtcg atgatgacat cagcaccgtc gaccatttcc atcccggcct ttccggccag
    30421 cgcaagatgg cccaggcagc ctgggagtcc ggctacaact tcaccgactc gaccatgcca
    30481 accggcgctg cgtcaatcag tccggcgccg ctccagtcgg ggaccagttt cctgccgtcc
    30541 acggtcagca caacctggtc ggattcagcc gggatcaagg ggatccagta ccggacccat
    30601 ttcaacggga ccgccagcgc ctggcagacc cagctcggca ccacaacgat tccggtcaac
    30661 cagaccgaga cctacggata cctgacccag aagactttct cggtcaccgt cactaccccg
    30721 ggcacgacct atgtcgagag ccgggcgatg gacgtgaacg gcaacctcag cgcctcgaag
    30781 gtcaccgagg tcaactacga cccgaacgcg atcgccgcgc cgcagatcac ttcgaccccg
    30841 ccctcgatca ccgcctcgaa cgcggcgagc ttctcgttta ccggcgcgat cagcggcacc
    30901 ggctacgaat gctcgcttga cgggggcgcg ttcagcgatt gctcgccacc gaagtcctac
    30961 tcgtcgatcg ctgacggcca gcacgagttc cgggtccgcc tgaagtcagc ggtcgcctcc
    31021 gggccggccg tcagctacca gtggcagatc gactcctcgg cccctcccgc gccgacgatt
    31081 cacaccggtc ctgccgccaa caccaaactg acggtcgccg atttcacctt ctcgggcacc
    31141 gaagccgccc tcgagtgctc gctcgacggc gctactttcg cttcctgcac ctcgccccgg
    31201 aatgtcttcg tcccgactga cggggtccac cggttcgaag tcaggcagaa gacgatcacg
    31261 ggagtcccgg gaacctccgc cgtttacaca tggacggtcg acaacacgaa gccccccgcc
    31321 ccggttttcg gcgaaggtcc ctcctccccg accaactcga cctccgcttc gatccagttc
    31381 accggcgaac cgggcgggac ttacagctgc cagctcaccg ggccgggcgg gctccagatc
    31441 aacaactgca ccagcccccg caacctggcc ggcctggcgc agggcgagta caccctctcg
    31501 gtttcccaga aggaccagct cggcaatcag ggcccggccg ccagctactc ctggacggtt
    31561 gacctgaccg ccccggtggc gccgcaggtc ggttccggac caccggcccg gaccgcgagc
    31621 cgcgatgcca cggttacctt caccggggag gccggcggtc acttcgaatg cgccctcgat
    31681 gatgcggact tcttcttctg ctccagtccg cggggtctga gcggcctcgc ggacggcgag
    31741 cacagcctgc ggatgcggca gatcgatgcc gccggaaata ccggcgccgc cgcctcgcgc
    31801 agctggacgg ttgacgcaac cgccccggaa gctccggggc tggatgggcg accgccggcc
    31861 ctgaccaatt ctgccgcggc ggcattcacc ttcaccggcg agcccggcgg agccttcgag
    31921 tgctcgctcg accaggctcc gttctcaggt tgcgccaccc cccaaactct cggcccgctc
    31981 gccgacggaa gccaccagtt ccgggtccgc cagaaggacg acctcgataa caccggtccg
    32041 gccgccagcc acagctggac ggtcgacacc acgccgccgg ccgccccgac catcgccccc
    32101 caaaccctgg aactcagtca gaaccgggac cgtcagatcg gtttcacggg ggagcccggc
    32161 ggctccttcg agtgccggat aaacgagggt gtctggagca actgcctgcc gctctgggat
    32221 gcgaccttcc ccggggccgg caaccacagc ttcgacgtga ggcagaccga cgacgccggc
    32281 aacaccggtc cgtccgcccg cggccaggtc acgatctggg gtggcccgcc gtcgacgccg
    32341 gttatcacca gcgcacccga ggggacggtc cgctcgacca gcgccgaggt gcggttcacg
    32401 gcgggccccg tcggggagcc ctactgcaac ctcaacggtg gcaactggca ggcctgcgac
    32461 ggagtctgga cggcgcagaa cctcgagcag ggaccccaga cgctggcgat ctctcagaga
    32521 aatgaagttg ccggccccgc tgtcgtcgtc cagtggacgg ttgacaccct cgccccgaag
    32581 ctgagtggca aggtcaccgc gaagccgaag ggcaaggtga agaagggcaa gccgcggacc
    32641 tggatcctga ccaccagcta tgatcgcaag cttgagaaac cggccgccct cgagttctca
    32701 ctctccaagg cgaagcccaa ggcgacgatt gcgccggtca ggtcacgcac ccgcaagtgg
    32761 gcgccgaagc tgaccatcaa gtcgacgccc aaacccgcct gggtgcgggt gctcgacccg
    32821 gtcggcaaca gctccccctg ggtccgcgtc aggtaacccc tcccgatagc cacctgccgg
    32881 attgcggggc tactgcggcg tccgggggcg caacaggacc gcggccagac cggcgctggc
    32941 gccgaccaga atgatcgaga ccgcgatcgc ggtgtggtag ccggacatga aggcaagctt
    33001 ggccgcctcg atcaggggat cagccgtccg gctcgccatc ttgccggcga tttcgagcgc
    33061 ccgtgccagc gactcctcgg cgttggcgat cccggccccg gtgacgccct cgatcggacc
    33121 gatcttcgcc gtgtagacga tcgccaggat cgaaccgagc acagccacgc cgaaaccgcc
    33181 tccgagttcg taggccgtct cctcgatcgc cgccgcctgg ccggcctcgt cggccgggac
    33241 cgaggaaatg atcacgtcat tggcggcgac cagggccacc tccagggtga agccgatcac
    33301 caggaaggaa ggccagagca ggtagtactg gtcggtcagg ccgagcgcca gcatcggaac
    33361 aagaccgagt gcggtgccgg ccagcccgag tgaaatcgtc cagcgggtgc cgagcctgcc
    33421 cagcaggcgc gcagcaagca agcctccagc cagggtggaa agcatcagcg gcaagagccg
    33481 gagcgccgcc tccaagggtt cgaggcccag caccagctgc aggtactggg ccccgaagaa
    33541 ctcgatcccg accagtccga acatggacag gaagacgcaa ccgaccgccg ccgtgaactc
    33601 ccgccgtttg aagagccgca cgtcaagcag gggctcaggc gcccgcagct ggcgaagggt
    33661 gaaccagaaa agggatccgg aaccgaccag aagcgagagg agcaccttcg gatcgaggaa
    33721 gccgtagcgg gcaccttcct tgaggccgaa ggcgattccg aggattccga tcacggtcat
    33781 gatcaccgcg aagccgtccc agggcggcgg ttcggccgcc cggctttcgg gcagcagcct
    33841 gattgcgacg gggatgatca caagcacgat cggcacgttg atcaggaaca cggcacccca
    33901 ccagaaatgc tcgaccagga aaccgccgat cagcgggccg atcgcggccc cgccggcgct
    33961 gaccgcgctc cagatcccga ccgcccgcac ccgctcctcc cggtcgcgga agacatcccg
    34021 gatgatcgac atggtcggcg gcatgatcat cgcgccgcca accccgagca gggcccgggc
    34081 cgcgatcagg gtttccgcgg tcggggcgaa ggtcgcccct agagatgcca gcccgaacac
    34141 ggccatgccc gcgaccagca gccgtttgcg accgagccgg tctcccagca ccccggatgc
    34201 gatcaggagc ggcgccgcga ccagcggata gacgtcaatg atccagagca gctgaagcgc
    34261 cgtcggatcg aggtcttcgg aaattgccgg cgccgccaca tgcagcacgg tcacgtcgat
    34321 caccaccacc atcagcgagg cgcagatcac cccgaggatc gcccagcggc gaggatgtga
    34381 ttcggcgacc cggtccatga tcaccaaatg aaatcaggag atggcttgcc acccccgggt
    34441 tggttcagtc tgccccgggg cagttgatcc tgccgtagcc accggctttg aagatgcggg
    34501 cgaacttcag gttgctgcgg acgaagtagc tgccgcggtc gagcagcctg gcggtacggg
    34561 cgaccgaacc ggtgcgcttg tagacacgct gaacccgctt catcgcacga atgtccgaga
    34621 ccgggatccg cggcttcggc aggtcgcccg tggcgagcgg cgagccaacg gcccgcaagg
    34681 catcaatctc cttgcggtgc tggagtttgc gatcaccgag ccccgagccc ccgatgatga
    34741 agaaggccgt gcggccggtg aagccggccg tgaaggccgg agtctcgcgg cccggtttga
    34801 ggctgcgaag caccgggatg ccccgctgct tctgggccca gtcggaaccg atgtatgccg
    34861 cggtgtatcc ctgcagggag cagatgtcct cgttgtagat gaggaccccg cggccgcagc
    34921 cggaaaaggg aatgacgcca caatcggcgt aggtgaaggt cgaccggttc cgttcggcct
    34981 cgacattcga gagcagcacg ttgtggtggc tgcgcccgag ccacaggttg tcgatcgccg
    35041 acccggacat gtcaggggcg gtgagctccc ggttcggtcc cggcggtctg gcgttgcatc
    35101 cccgcggccc ggtcagcttg ccgtcggggt cgaccagcac aggcgagagg ctggccggcg
    35161 agccgttggt caggcgggcc aggcattcgc cgcctggccg gatcgaccag atctcggtcg
    35221 tctcggcgac ttccctttcc ggaccacggg ttgactggaa gacgatgcgg cccggagcgg
    35281 accagtccgg ccggcgctcg tcggcggctc gcctgtcgag gctggtcagg tcgcgcgcac
    35341 cggttccgtt caggttgacc aggcggatcc ggctgaagtt gatgcacgaa tagtcatagc
    35401 tccagcagaa gtcgttgtct ccttcactgt caacctcact gaagacgatc cggtttcccc
    35461 cgggcgagaa gtccccgccg agcgagttcc cggcaatctc gcggctcttg ccggtgtcga
    35521 tgttcagcac ccgaagcgaa gccccgtagc cctcctcgaa agaagtcccg tcgtatcccg
    35581 ggtcgagcag atagagcaga tgcttcccgt cgggcgagag cgaaggttcg gccaggctct
    35641 tccagccctc ccagggacgc tggcgctgtt tcagccgcag catcaccttg cgggatttgc
    35701 cgttcgtatc catggtgaag atccgggcct cggccaggct ggagttctcg cggaaggagg
    35761 cggcgatgat ccggccacct gccgtgaatt ccggatcggc gtagcgttgc ttcctgttcg
    35821 aggccagcag cttgcgggct ccggtgccgt cgaagttcat cacccagaca tcgaacagag
    35881 agtcatatct gtcgagccgc ctgaagccaa aagtgatctt tgtcccgtcc ggggaaaccg
    35941 aaggctcgac ggcgccgagc ccgtcgtttc ggggaaggcg gtccttgccg aagaggactt
    36001 tccgttcggt gccgtcggcc ttcatcgtga ggatgcggcc gccgttgctg aacaccacgt
    36061 ccgccttcgg cttcgccgcc tgagcctggg cgccaaggcc gaggcccatg gtgagggcga
    36121 taagcgaaaa gaggatcgtg cgacggccgg tcatcttcac ctccggccct ggcacttgac
    36181 catggcggcg ggaggcagct tcgaaccggg gtcgagggac ttgccgaccg gtcgcagatt
    36241 gtcgatgaag gccaggtggt ccgagaaggt cgaggccctg ccgccgatgt cctcgacgtc
    36301 gagcatcgac tggccgatcc caatgtcaac caggccggtc cgcagggtgg tagtgacaaa
    36361 ttttcctcgc gctgtccagt gccgggtcac cctgactccg cgtcgccgga tcacttgctc
    36421 cctgcctcgg ggtggcaccc tgacctcgcc gaggccaccg caggcgaagg caatttcgat
    36481 cgtgatcggt cgccggcaat ccgagctgtc gaaaaaggga cagtcgccgt agcggtagat
    36541 cggggaaccg aactcgtcct gggcgtaacc gtcgaccttg gagagcagcc ggtttgcgac
    36601 gattggcccg gcccagtagc gcgggcgggg gtcggtgtcg atcgccctcg gcggccgcgc
    36661 ctcggccagc ggtttgcgtc cggccgcacc gcatttcgca ccggggtcgg ttcgcacctc
    36721 ggtctccggc gcccaggcgg gcatgaccga ctccggtgat ccgttggtca gccaggtcag
    36781 gcattcgccg tcggagcgca ccgagtagat ctcgttcgcg tcctgccgct cctccgggaa
    36841 gttccggctg ctggcgaaga tgatgtagcc gccgcccgga ccccagtcgg gtgagctgta
    36901 cccgccgtcc ccggtcacga tcaactggcg atcggaaccg tcgaccccga tcgtccagac
    36961 cccggtcctt ccgtcaccgg aatgagcgca gtgggcgtaa ccctcggtgt caacgcacat
    37021 cttcgtgaag gcgacccgct cgccatcggg agagaacgag cccgcttcgg cgcccgtcac
    37081 cagtacccgc tctgccccgc tgaccaggtt ccggatccgc agctggacca cgtcgtgacc
    37141 gtcatcgttc tcgccgaagt cacgggtgta gagcagcgac ttgccgtcgg ggcttacttc
    37201 ctgggcctgg agcgtgtcgt cgttgtagtt gccgaagaac cttttcgaga cgaggacctt
    37261 tttcttcccg gtcgcggggt cgagcgcgag aatccggaag gtctcggtgg tccggctgta
    37321 gccgtagcgg taatcccagg tggtgaggag gagccggccg tcgggcatga agtccagcga
    37381 ctggatttcg gcgtttatgc ccttgaccgc ggttgccccc caaccgtccc ggtcggccag
    37441 catcacctgc tgcgaatgtg cggcgggccg gtacctgacg aaggcgagtt tttcgccgtc
    37501 cggggaaatc gccggctggc tgtcgccgaa gtaatggatg tcggccagat agttgcggcg
    37561 gagcaggtcg gtgcccttgc gggtcagaac cttgcgtccg gatccgtcgg ccttgatcga
    37621 aacgatccgg ccaccgttct cgaaaacgac ctccgccagc ggcgccagcg gcggcgtcgt
    37681 cgcctgcccg ccccccggcc aaagcaccag gcacccgaac atcccggcga gcagcccggc
    37741 caggaaccgc aacctcaccg aatcagctcc ctgaagtccg agcggacatc gatggtgccg
    37801 ggtttgttgg cccagcgtct gcattcgacc gacttgaccg gtcccagccg ggccagatca
    37861 cggctgaacc tgagccaggc ctgcgccgct cgcctcgagt agctgaagct gatgcccatc
    37921 ccggcccgga agatcccggc ccgaacggcc gccttcttca gggattgagt ccggcggaag
    37981 ctgcgcacca tcacccgcgc ggtgtgcacg gtgtgccagg cgaagaccgg ggccccgaag
    38041 ctggccggcg gcttgctcgc cccgtacgga cgcagatggt cgatcgcctt catgtgccag
    38101 gcccgcgaag tcgctttgcc gtcatgtcgc cagggcgagg taatccagac ctcacgacca
    38161 tcggcgagaa agatggttga aagcgacggc ctgacgctgc cggtcatgac tgccagtcca
    38221 cccttgcgac ggaccattcc ctgataggcc ccgtttccga gctctccctc gaggttgcgc
    38281 tcgcaggcat gggccgatcc gattgcgatc gtggccgggc aacggttcct gaaccaggaa
    38341 cagtcccagt aagtgaagct tgcgttgctt cgatcgacga aatagtcgct gaacaacagc
    38401 agcccgaagt tcctgcccag ccacagaggc gtcacctcga ggtctgcgat tttcgcgggt
    38461 ggttcgacct ccgcaactcc tctcgaaccc atcccgaggc cgcaaccggt cggggaggta
    38521 tccggggtca atccccagga accggattca ctggccgggc tgccgttggt gattcggctc
    38581 aggcaagagc catccggctc caccgaccag atctcatcgc cgaagattgt cgtgaccgaa
    38641 gccttgttgc tggtcaggag tattcgcgag ccgtctccgg accagtcggg ctcggcgaaa
    38701 ctccagggct gaatcttccc gagcagcaac cgcgggttcg aaccgtccgc gtccatcaga
    38761 tacagctgct gggaagacga gcagctgcct tcgtaacaac tttcgttcac cttgtcgcgg
    38821 gtcgtcgaaa agacgatcct ctggccgtcc ggtgaaaatg agggatcgaa ggccgacttc
    38881 tcgatcacct tcgatttccg ggtagaaagg tccaacgcgt cgattcgggg ctgcgcgttt
    38941 ccgttcatcg ggatgcgggt taccaggagc tccgacccgt ccggtgaggg ttcgacttca
    39001 gacgcgatcc agaggtctcc ggatggcttc tcgttcttga aggtcgtctt gccgacttcg
    39061 attaccttga ggtcgctgcc gtcgggaagc atcgagatga tccgggtcct gaccgcatac
    39121 ggccagccgg ccggaaccag cttgacgatg tactcgacct catagatccg cgaaccatcc
    39181 ggtgcccatg cgaagccgcc ggtgtaggtg gtcagctgga gggtctcgat ctcctccatt
    39241 gccctgatct gggtcggagt tccgccgtcg acgtcaatcg agaaaaggcc gagcttcgac
    39301 cggtcaaccg atccggatga atcatcgtcc cgcgagaaga gcacggtctg cccgtccgga
    39361 gagacggccg gatccgaatc ccggatcccg gtctcgatca tcgtctttgc ccgccgcggg
    39421 gtcaggacct ttcgacccgt cccgtcggcc ttgatcgaga cgatccggcc gccgttctcg
    39481 aagacgatcg tggccgcggg cagctgctcc ggcgggatgg ccgcggcccg gtctgccgcg
    39541 aggaggaaag cggcaagcag cagcgccgcg gcgaaaagcg cgatgagcga tgcgggccac
    39601 cggttgtggt ggttatccct gccccctgat gtcgacatcc tgcgactcat ctttctgttt
    39661 cccggatccg ttccggtttc cctgtagaag cagggattct accgtccggt gacttgaaac
    39721 tgttacgtgg gtgtgtcctc gcccgtcacg gtaaagctga cggatgacac accccgtaac
    39781 acggtcggat tgagaagtat cggggcgccc ggatttgaac cggggacctc accgacccga
    39841 acggtgcgcg ctaccaggct gcgccacgcc ccgaggaaga atgagcctac agaaattgcg
    39901 gcccggaacc cgtcccggcg gcggctactg gacctcgccg atcgccgcct gcatctcggc
    39961 caggtcgggc gcgtcctgaa ggatctcggt cccggacggg ccgttgacca ccgcagcggg
    40021 ggcgtcgcgg atcttcaggt cgatcgcgat ctggtcctgg gcctggagat cggcgttcat
    40081 ggcgctgcct tcctcgagac catcctggta atcctgcttc cactgttcga tctcgaggtt
    40141 gtcgatcgac tccgccagcg tggtcaggaa gtcgtcgtct atgccctgct ccttggcgag
    40201 atcctggttg cggaacatca ggtaggcgta ctgccagccg tagccctgct tcatcgctgc
    40261 ctcgatcccg tagaagctgc gctccgtcgc attgcgggtg agcgaacggt tgcggtagag
    40321 aagcttgagg gtgccgtccc ggaccggccc gctgaccagg gcgggaatcg tgtcgaggaa
    40381 ctggtccttg taggtcgagc tctgggcgtc gagaaagacc tgcatctggg ccggggcgtc
    40441 atccgagccg agccggtcgt cgagctggcg caccccgccg aagatctggc gcatgtcctg
    40501 cttgccctgg acgttctcgt attcgttgtc gggtttctgg atcgccatgc cgaccagggc
    40561 gatcagcagg gcaccgatcg ccaccacgag gatgatcagc gtccggaact ggccggaacg
    40621 gcgctgcctg aaggcgctgc tcatctcagc cggttaccga tttgtagtac cgcaccgcaa
    40681 gaccggtgat cagggttttg aaggcctgtt cgagctccat gtcctcctct tcggagaaac
    40741 ggaccagacc gcgctcctcg atcgccacgc ttcgatcgat cagttcgtcg atcagttcgg
    40801 gattacgatc ggagaaatag gcacccatcg aatagagatc ggtgtccatc agagactcga
    40861 tgaaggggtc gctggcggca gcgacttcgg tttccggctc ttcagccacg gaaccatctt
    40921 agggacaggg gcgatcccct tatatggtgg ggccgtggcc gaccgcaacg agatcatcgc
    40981 cttctgcgac gaactgctgg agatcgagag cttcggggac tacggcccga acggcctcca
    41041 ggttccgggg aaggccgtga tcgggaaggt cgcgaccgcg gtttccgcca atctcgcctc
    41101 gatcgaggcc gctttggcga ccggcgtcga cctgctgctg acccatcatg gcctgttctg
    41161 ggattttcac ccccgggcac tgaccggggc gatggcccgg cgacttcgcc tggccctcgg
    41221 cgcggacctc aacgtggccg gctaccacct gccgctggac ggacacccgg aaatcggcaa
    41281 caacgcgctg atcgtccgcc agctcggttt cgaacccgtt cccggcgaga tcggcgaagc
    41341 gaaaggccgc catatcggtc agatcggccg gcgggcggaa ccgatcccgg cgactgcgct
    41401 tttcgagctg gtcgggcggg aactcggccg ggagcccctg gtgttcgctg aaggcccgga
    41461 aatggtctcg accatcggcg tggtcaccgg tgccggggct tccgacattc acgaggcgat
    41521 cgcgctcggg ctcgatgcct acctgaccgg cgagccctcg gaacatgtga tggccgacgc
    41581 ccgcgaaggg gcgatccact tcatcgcggc cggccactac gcaaccgaga tctgcggaat
    41641 tgccgccctc ggcgacctgg tcgcccaacg cttcgaggtc gaacatgaat tcctcgacct
    41701 gcccaatccg atctgatgtg gcaggatcaa tcgatcaaac cgctttgcgt cgccacatag
    41761 ccacatagga gagagacttg tccagcacct acacgccgac caaccgttcc cgaaccctcg
    41821 ctgacctgct gccgaactcg gctgccaggc acggatcgga cggtgccttt ctcttcaaga
    41881 acgaggccgg cagctgggag gagacctcct acgaaaaggt ggcggcggag gtccaggcgc
    41941 tcaccctcgg tctgaaggcc ctcggcgtcc agcgtggcga caaggtcgcg atcatcggca
    42001 acaccaggcc cgaatggacc ctcttcgact tcgccgcgat gagcaccggt gcgaccgtgg
    42061 tgccgatcta ccagagcaac tccccctccg agtgccagta cgtgctcgag cattcagagt
    42121 cccgggtcgt gatcgccgaa aacgaggatc aactggccaa gatccgcgag gtcagggatt
    42181 cactgccggc ccttgaaaac gtcgtgatca tgatcggcga agccgacgac gcgatcagca
    42241 tgaacgagct gcgggcgaag ggtgattccg gcagcgccga ggactacaag gcgagctacg
    42301 aggcggtcga acccgacgac ctctgcaaga ttgtctacac ctccggcacg accgggccgc
    42361 ccaagggctg catgatctcc cacggcaatt accgggcgat gctggacatg gctgactcgg
    42421 caagcatcgt cgaggaaggc gagaccagct atctgttcct gccgctcgcg cacgtcttcg
    42481 ccctgctgat ccagtacggg atgatcgaca tcggcggccg gatcgccttc tgggagcgtg
    42541 accagctgaa aatcgtcccc aacctggccg aggtcaagcc cgagtccttt ccctcggtcc
    42601 cccgcatttt cgagaaggtc cacgacaccg cggtcgccac ggccgagggc gagggtggcc
    42661 tcaaggcggc aatctttcgc tgggctttcc gggtcggcaa gcgttaccgc aaggccgagg
    42721 cccagggcaa aaagcccggc ttcctgctga ccaagcagtt caatttcgcc gacaaggcgg
    42781 tcttctccaa gattcgggag atcttcggcg gcaacctgcg ggtcgcgatc agcggcgccg
    42841 cgccgatcga tccggagatc ctccgcttct acgaagcggc cggagtgccc ttgttcgagg
    42901 cctggggcat gaccgagacc tcgaccggtg gcaccaccaa catgccgggc gaaaaccgga
    42961 tcggcacggt cggcaagccg gtcgacgggg tcgaactcag gatcgccgag gatggcgagc
    43021 tgctgatcaa gggcgacaac gtcttccagg ggtattacaa gaaccccgag gc
//
LOCUS       DAOQKN010000003        39509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_381_length_39509_cov_5.958281, whole genome shotgun sequence.
ACCESSION   DAOQKN010000003 DAOQKN010000000
VERSION     DAOQKN010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 39509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39509
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_381_length_39509_cov_5.958281"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            235..3789
                     /gene="metH"
                     /locus_tag="PLJ64_00380"
     CDS             235..3789
                     /gene="metH"
                     /locus_tag="PLJ64_00380"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HNC91979.1"
                     /translation="MAERDYLSAISDHIVVFDGGMGATLEMFDLTQEDYGGLAGKCHE
                     ALVLNRPDVIEGVHTSMIEAGAEVVETDTFQASRIKLDEWGLADHTEEINVKAAEIAR
                     KAAGPDRFVAGSIGPTGHLPASDDPTLGQIRFPELVTVFTEQAGGLVKGGADLIIIET
                     AQDILEVKAAIFGARAAFEEIGRSVPIQVSVSLLPNGGKMLLGTDIQAVLTTLEALKV
                     DVIGLNCSTGPEDMRDAIRYLGENSAVPVHCIPNAGLPLQGPDGETIFPEKPEPLAAT
                     LGEFVERYGVGVVGGCCGTTPEHIRAISERVKDLKPGPRPEPGEIRVSSMMTSTALVQ
                     EPTPTMVGERVNSQGSRKAKELLLADDYDGLLQVAEDQVEGGAHVLDVCVALTERQDE
                     DEQMRQVVKRISLTQPSPIQVDSTEPDVIRTALEQIPGRAIVNSVNLEAGRDKLDTVA
                     PLAKAHGACLIALTIDEVGMAKTADRKVEIARRITEMVCDEHGLDREALIFDDLTFTL
                     TTGDEEWRPSAIETIEGIRRIKAEIPGVKTSLGVSNVSFGVGQPARAVLNSVFLHHCV
                     EAGLDLAMVNPNHIKPYGEIPAEEIELTEDLVFNRREDALERFIAHFESKGDEAEEEA
                     EDATAGMEPEEALHFMILRRKKEGVEDWIDKSVEKIGAVPTLNDVLLPAMKEVGDKFG
                     AGELILPFVLQSAEVMKKAVSQLENYLDRIEGHTKGKVVIATVFGDVHDIGKSLVNTI
                     LTNNGYTVIDLGKQVPVDTIIDAAIEHEADAIGLSALLVSTSKQMPIAVQELHQRGLD
                     YPVLIGGAAINRDFGRRTLYPHGKESDEVYEPGVIYCKDAFQGLDTMDALIDDEARAA
                     LIDRLKQEAEEYRNKPVIVEDLPPLDDDSVRSSASTDAPIPSPPWWGVREVPVDFDEV
                     FDHLDLHVLFKLHWGGKGVKGEEWDKLVCEEFRPRLERMWREQDYLHPKARLGYFPCN
                     ADGNELVIFDPEDQDREINRLVFPRQPKHDRICLADFYRPLNDEGIRDVVALQGVTVG
                     PEVTELMEQLEKDGEFAEQLYTHGLGVQSAEGLAEWLHYKVRQDLGIKSSQGRRYSWG
                     YPACPDQSEHEKVWELLDLGEIGMYLSGGYAVMPEQSTVAIIAHHPQAVYFGMKSGFI
                     PKGEAPDEVIAESDRSANPPEADPDEGPTEAEGEPATA"
     gene            complement(3779..4189)
                     /locus_tag="PLJ64_00385"
     CDS             complement(3779..4189)
                     /locus_tag="PLJ64_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91980.1"
                     /translation="MFLVAALASAALLAGGSPAGAALQGSPKPLKVKLVTFDGKQRLK
                     VKRKVRLIASCSKDCNARVKLRLVTPVTTVRGGGSIGLEATGTWTIGVILSPSFRSYI
                     RQNITRCRLVANFSAVDIETGKTVRKVKTFRFRR"
     gene            4366..6348
                     /locus_tag="PLJ64_00390"
     CDS             4366..6348
                     /locus_tag="PLJ64_00390"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HNC91981.1"
                     /translation="MVPTAAASSAPHLSLPAGDSTDNGNVYLGDSSVITVTVNSDGSS
                     PLDITSVSIYAFPGNSEYFSIEQDNCSGQSVESGDHCTFQVRFTASVVGHKFTSVRID
                     SNDVESGGMKFFGISGTGINPGIDADAASHNFGTVQVGEYGTDKTFTIENDGTSDLNA
                     TATITGTDAAEFFFSSQPNCASVTPDSTCQIIVNLRPLGSAGPRSANLVIANNTKNEN
                     PVTIPLTATASAPHFTSEPLGLGFGQFDLFDDPPATRPLTIKSDGTADLKINGMVIDP
                     LNSNSNAFTLDQGECVDDLAIAPGDDCTLNITFKAGSFGEGSYNTSLLVFTNNGDQSV
                     PVSAARTSGGMSTDSSADVGDVELGQTGSATLTLTSTGTAPLKMSPPVIDGPDAGMFS
                     VDQPSDCNGLASGQSCEVTVHFTPTSLAGKQAGLTLSGNFGSTGVLLTGSGTQAFVNV
                     PGDITFPTTIVGKQVTWDESLISNGSAPFSLGSLTIEGADASMFSVETTGSCASVAHG
                     DECPYSITYKPTSTGSHEAQLKITGNLAGGSRTIPLSGTARNLTPAKVSLKLTGAKQV
                     KPGKTVTLTARISKSGEEQTGDLTLKTVVPKKMAASVKPVRIPHLGAGNRTVTRKITI
                     KVRRSAKSGQKLKVEATVSGGGLNKAGKAGKTVTIR"
     gene            complement(6341..6751)
                     /locus_tag="PLJ64_00395"
     CDS             complement(6341..6751)
                     /locus_tag="PLJ64_00395"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNC91982.1"
                     /translation="MAAETTHRLRVRYNECDRVGIVFNSNYLVYTDVAVTEFWREHLG
                     GYSEFNEIGLDMVLAEANLRFRQPLEFDDEFDIRVSVGGMTARSMTIEFEFTRSGEAI
                     AEIEISYVCVSSTEHTPQPIPDSIRTVLEGDSAT"
     gene            complement(6741..7652)
                     /locus_tag="PLJ64_00400"
     CDS             complement(6741..7652)
                     /locus_tag="PLJ64_00400"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC91983.1"
                     /translation="MTRQDQEKKLTVDLPQGTVAFRDEGEGPVLLFSHGIFVDSSLWD
                     GLVERLVPAGFRCVRPETPMGAHRIPLNPETPLSPPRVADLLADFIDALDLGPVTVVG
                     SDSGGAVSQMFAAQHPDKLAAMVLTNCDALEVYPPFPFTLLPPLARMPGGIAPLGLLM
                     KLRPVRWATYRMLTADPIPDRQLKAWLGPTGSNPGIRRDVNKVIGGLEKNQAIDAAEK
                     LRGFEKPVLFAWGESDRFFKPELAERLAAMIPNSRIEKVEGGRTFVALDDPEKVSNLI
                     VEFMNETVAPAGRTDAQRSTLAGADGR"
     gene            7714..8325
                     /locus_tag="PLJ64_00405"
     CDS             7714..8325
                     /locus_tag="PLJ64_00405"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC91984.1"
                     /translation="MQAARTQIERSDATRRALIDSARNLFGSRGYAAVPLDEVVSAAG
                     VTKGALYHHFPGGKKDLMEALYETVEADFTERVTAGIAPRLAEGEVDPLDIMGAAIEE
                     TLDLSMDPELQQILLLDSLAVLGWSRWREIADKYSLAVVKSLLAAAVEHGSMKPLPTE
                     SLANLLMAALSEAILMIPRAGDQEQAKADASRSLKAIIDGLRA"
     gene            8351..9145
                     /locus_tag="PLJ64_00410"
     CDS             8351..9145
                     /locus_tag="PLJ64_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC91985.1"
                     /translation="MSEFTASYRGGDGPTLVLIHGFTGTWEIWDPIIAPLEQHYDVFI
                     PTLPSHAGGPAIEGDPTLELLLDGAEALLDEAGIETAHIVGNSLGGYAALRLAARGRA
                     KTVNALAPAGGWAEGDPVIAQTMDFFQQMHPLVVAAAEHVDDAVATPEGRAMLLQFLT
                     EHPEVVSPDLAKKILLGGAGCTEAIALTEMGRTADWAFDLNAIECPARIVWGTEDKVL
                     GWPIAAARYQEGLPDADWVILEGIGHCPMLDVPERTVELITEFAKD"
     gene            complement(9291..9974)
                     /locus_tag="PLJ64_00415"
     CDS             complement(9291..9974)
                     /locus_tag="PLJ64_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HNC91986.1"
                     /translation="MFEQMKTPPVVSAEEWDAARMELLVREKEVTRARDALAAERRRM
                     PWLKIEKDYEFEGPEGRVGLIDLFEGRRQLVLYRFFFDPDVSGHPDAGCPGCSFGADQ
                     VADLRHLNARDTTFAHASRGSQENIRRLKEKMGWEQIPWYTVIDDFDRDMGVGEWHGT
                     NAFIRDDDGTVYRTYFIDARGDEHMGSTWAHLDLTALGRQEEWEDSPEGYPQTPPYQW
                     WRRHDEYGS"
     gene            complement(9977..10444)
                     /locus_tag="PLJ64_00420"
     CDS             complement(9977..10444)
                     /locus_tag="PLJ64_00420"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNC91987.1"
                     /translation="MTVSDQSVHLVKVVQAPPEMVYRAFIDPDELVRWMHPDAFTGVS
                     ASNDPRVGGRGELIHALDGEEVGGFNWQYLEVVPGRRLVMDWQFGPAGADPDGHHSRL
                     TIELREAGPGATEVSLTHDRLGDVPPGGPTGVSTGWTQALASLGRHFEAEGDE"
     gene            complement(10441..10782)
                     /locus_tag="PLJ64_00425"
     CDS             complement(10441..10782)
                     /locus_tag="PLJ64_00425"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNC91988.1"
                     /translation="MVEDQKEVDRVFHALSNQTRRGMVRQLSGGECSVGDLARPTRMS
                     FAAASKHVQVLEKAGMVNRRVEGRQHICSLRPDPLREASEWLSFYEKFWAGNLDRLAR
                     VVEADMKKEKK"
     gene            10928..12967
                     /locus_tag="PLJ64_00430"
     CDS             10928..12967
                     /locus_tag="PLJ64_00430"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNC91989.1"
                     /translation="MRKLVTFVAVLLGLLAVPATASALKFEAAPGTPLAPATSVIDPS
                     PVMPPGFSPTDIATGDFNKDGYGDIAVITDYISVQGPDGPEISPLSKNIYVYLGSADG
                     SLESSASNPIGADSPIAGGRFMIRAIDVDGDDDLDLVTGSGGNPTIIEIYQNGFNGTH
                     EAFATVPGQTLSLDDSESAAGYRSVSLGDQDDDGDVDMALGLYDNRYVFIRNLSGLDG
                     LEIPNPSVEVSRDDPQDIDGITSTAIGNFGDGTGPDAFGDLVMVSSPGFRAENQPPDR
                     LLYARGDGASSFASPVELLRTGTDEYLGWVSKADLNGDQYDDIVFKVQTATGENIVRA
                     ALGSADGPVIQAGEGSSISVYGAQQPAPGDFNGDGDQDVAIPQFVDDGFQVGLGDGSG
                     RLALDFAGPFALAAIDSADFFPQASATLDANGDGRLDFAAASGHSGDADQARGVAVML
                     NKPSSGISVSPTSIHFGDVPFDAELIAPVPVTIKSNGDLPLNLGQIGFSGPATGGFSL
                     DRGNCPNGNLASGSTCTLEVSMKQNQSGYFNGFLDITSDATGDPVRIEVIGQVGEAPP
                     TARGLSLKVKSAKKVKAGKKLVVSAIVRNSGKARSAHFTIRATAPKKMTGRIKFSPLR
                     NLSLDGRSYTTFFFKVPVKKQAKGVLRVKVSLVAKNRKIVRKTGEVRIVKKARRR"
     gene            complement(12983..13711)
                     /locus_tag="PLJ64_00435"
     CDS             complement(12983..13711)
                     /locus_tag="PLJ64_00435"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HNC91990.1"
                     /translation="MKHRHLIALALAAAILPAPVASGATERKVIGRSVDGRPIVSWRQ
                     GDPKGDFRVLVVGSVHGDERQGMRVVARLRQNLAGRGGPKGIGGWTVKTANPDGTALG
                     TRRNARRIDLNRNFPFRWDGSLNGGYNSGPRPASEPETRAIMRLSRRVGFDLAVWFHQ
                     PWGRTLAPCDGQRRWAFLYARLSGLGTGGSCDHGRYPGSAVSWQHQATGTTAFVVELG
                     PGLQPRAVINRHARAVIRLAKRMK"
     gene            13750..14322
                     /locus_tag="PLJ64_00440"
     CDS             13750..14322
                     /locus_tag="PLJ64_00440"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HNC91991.1"
                     /translation="MSELEDDGLTRCAWANGSQMMRTYHDEEWGCPIVGEQDMFERLS
                     LEAFQAGLSWATILRKRPAFREAFRDFDLEYCAGLTDADIEELMGNEGIIRARAKIEA
                     TRSNAAATIALREEGGLEGLIGSFVPERMPEPANQSEVPTSSPESTNLARELRAHGFR
                     FVGPTNMYALMEASGLVNTHLTSCFRRDQV"
     gene            complement(14319..15332)
                     /locus_tag="PLJ64_00445"
     CDS             complement(14319..15332)
                     /locus_tag="PLJ64_00445"
                     /inference="COORDINATES: protein motif:HMM:NF022169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
                     /protein_id="HNC91992.1"
                     /translation="MAADSSAKSLPAPGWYPVPGRDRDTTVERHWDGEVWTDEVRTAP
                     VGSSLATYRRHFWGFVRNGNWKLLLVLILTLAGAAALWGTDRKADLVGGIQILAVPLA
                     AAATFVTMVAFLRFIGRRVGFDRIPAETRREIVKWGIASGVLAFALAFAVEFFLPRVF
                     GDNLKDDPGWSVLDGPAEETGKLLLPVILWFRGRFRLPRQGYLLVLVSAATVAVMEGT
                     EYAVQPEIYQPMRPVLEIMHPLLTGFVAAVAWQAAWHRKSIFTAAAVGAWVLAMAAHS
                     TNDVIVLSHHVDGSIARMTNFVSIAVIVLMYLLQKHSARQMVPPDKVGEVSPRWRPVA
                     GPG"
     gene            complement(15362..16303)
                     /locus_tag="PLJ64_00450"
     CDS             complement(15362..16303)
                     /locus_tag="PLJ64_00450"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNC91993.1"
                     /translation="MGDLILYAIPFFFLAMGLEYLTLRHAAHEHGEAQAANPDAPIGF
                     EAKDTATSLSMGVGHLFIAGAWKLVVLVIYAAIYEVSPIQLSPSDWWTWVLLFFADDL
                     AYYCFHRSHHRIRLFWAMHVVHHSSEHYNFSTALRQDWSPFSSIFFWMPSALLFPPWM
                     IYLAFSWSLLYQFLLHTEAIDRLPRPIEFIFNTPSHHRVHHGSQEQYLDKNYGGILII
                     WDRMFGSFEPEEERVRYGLTKNIKTFHPVKVAYGEYMNIWRDVRAADSWRDRFGYAFK
                     GPGWKPEPIAADDERIATDSHRRSDGTDRDRRLAAAD"
     gene            complement(16373..17059)
                     /locus_tag="PLJ64_00455"
     CDS             complement(16373..17059)
                     /locus_tag="PLJ64_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNC91994.1"
                     /translation="MPGSGTETVSRRVHDELRRQIVEGELAPGDRIPSERVLAEDFGV
                     NRHAVREALKGLQAAGLIRITHGGATRVLDWRDAGGLEVLLDLGGDPLEPPAEIVRSV
                     VEMRASIGVDAARLCASRASQSERDQVEQLGESAAAMIGTGDEAGLDEAFAAFWLAIV
                     AGSGNIAYRLSLNSLLKAMLTFGDVADRIRPADPRIITRLGNAIGAGDPETAAEVAGH
                     MLRSDIDRVA"
     gene            17084..17584
                     /locus_tag="PLJ64_00460"
     CDS             17084..17584
                     /locus_tag="PLJ64_00460"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNC91995.1"
                     /translation="MARLRLLFTGALLAFGLQTLIRRLLLTQFRRSVEALNQGDYGPL
                     LDGFADDAVLHFNDAPHRWAGEHRGRDQIETFLKDFVAAGLQGEILDLWTSGPPWAMT
                     AVARFDDRSTGPDGQELYSNQTMIMVRTRWGKVTEQRDFYVDTTSMIDFDRRLTELGI
                     LPVADG"
     gene            17597..18397
                     /locus_tag="PLJ64_00465"
     CDS             17597..18397
                     /locus_tag="PLJ64_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC91996.1"
                     /translation="MTPGFTPFHHGGSGPPLVLIHGFTDTWRTWDLVIPRLERDFEVL
                     AVTMPGHAGGPAIEGKLTVDTLPSGIEAAMEAAGFTHAHLAGNSLGGWVALQLAARGR
                     ALSVTGLAPAGGWSAEFQNQWALDYFITQQDLVKKAAPYADQIASTPEGRRQATRFIV
                     SDIGLITPEMVVHQLIGAASCPAAMPMVELAREQGFELDAEQIECPVRFVWGTADLLL
                     RWPETAVRFREEWLPAAEFIELDGIGHCPQLEVPAEVAELIAGTARLA"
     gene            complement(18414..19103)
                     /locus_tag="PLJ64_00470"
     CDS             complement(18414..19103)
                     /locus_tag="PLJ64_00470"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC91997.1"
                     /translation="MARATSVFEKQAATYRGPREKLIPQMERFYGMVPEAIGLAEPET
                     GREGQPLRILDLGAGTGMLSAVVAEAWPDAAFTLFDFSPRMLEQAKESLGEAVRVLTG
                     DMYEGIPAGPFDAVISALAIHHLTDEGKRSVYEAVFGELRPGGIFVNAEHVLGETPAL
                     QQHYAKWHRRQAFEKGLTESEWADTVERMSHDHLTPLSTQLGWLEEIGFTDVDCLFKD
                     HGFAVLFGRRP"
     gene            complement(19131..19730)
                     /locus_tag="PLJ64_00475"
     CDS             complement(19131..19730)
                     /locus_tag="PLJ64_00475"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR00080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HNC91998.1"
                     /translation="MIEQLAVEVGDRRVLDAIAAVPRDLFVPEDQRRFAWENRPLPIG
                     RGQTISQPLVVATMCELLELSEDDLVLDVGGGSGYHAAVLARLARRVISIERQPELVE
                     AARRSLAAAGVVNVEMICGDGSVGCPGEAPFDAINVAAAVRGEVPEALPGQLAEGGRM
                     VVPVGRRHQWLELWRRREGRLSRERMVAVAFVPLVEEGD"
     gene            complement(19741..22197)
                     /locus_tag="PLJ64_00480"
     CDS             complement(19741..22197)
                     /locus_tag="PLJ64_00480"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNC91999.1"
                     /translation="MQVSSSGAMRPLGRLFGVLFGLGAVLMLLFAMPLAGRADAAKKP
                     PRVTIRTTQYGIPRILADNPYGLGYGYGWAIASEQLCTLADTYTTVRGERSLYFGGDV
                     NAPNGVTNLDSDFFWKRVRKEGTVQKMINLKPPNGPLPDVKQAVRGYAAGYNAYLRKT
                     GVGKLPDPRCRGKAWVKPISVLDVYMRFYQLLGYGSTDPSMAGIAEAQPPLAMTRKNG
                     SAPADEVNPADITTSDFPKGFGNFTGGLGSNAVALGKNSVKGGGGLLLGNPHFPWHGA
                     QRFFESQLTIPGRLNVSGASLLGVPVILIGHTRTMAWSHTVSTARRFILFREALDPTD
                     PTRYIVDGQSKPMKATTVRVKDKDGMVHTRTLYSTEHGYMTNQLQGSNLFGWATDYGY
                     SLYDANTGNFRMINHFYSMNQARSVPEVLKILKKYQGIPWVNTIASDSKGRALYADIG
                     AVPNVTDGRVADCNVGLGIVTWPSYRVPIFEGSDSNCDMSKKAPGAAGPGILPPSQMP
                     YQVRSDYTENSNDSYWFTNSKHTLDGFPLIIGQEGIAQSERTRLGHKMVGEKLAGDGR
                     FTLASLKAMEFNDRVMSAELLLDPVIDYCNANPSVVGSGGPVSTASACDALEAWDGRN
                     NLDSKGGLFFQRFAGRLYSNSTDPVYANPFDIADPIATPNGLNTLNSALPIALADTIT
                     EFQDQGIPLTATRRKYQTVTRNGVKIPIHGGDYEPYGSFNTIWGPWIPGKGITEVSDG
                     SSFIQAVRLDGSKCPRASMILTYSQSENPKSKHYADQTKLFSKKGWSLDRFCPNQQKK
                     SPGLKVKRFGGGAKAESRGY"
     gene            22339..22809
                     /locus_tag="PLJ64_00485"
     CDS             22339..22809
                     /locus_tag="PLJ64_00485"
                     /inference="COORDINATES: protein motif:HMM:NF016701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HNC92000.1"
                     /translation="MESSAQDQTLKPAEHMTGECMCGKVKFEVDQPLIGAALCYCKRC
                     QRRTGSGVSATGLTQPGSFRITEGEDSVTSYEPGDGGFNKYFCRNCSGQLFTSHPDNP
                     EILAVRLGALNEDPGIRPAAHQFTAYAAPWFPVPDDGLPKFPERLDWTQVLGQQ"
     gene            complement(22895..23392)
                     /locus_tag="PLJ64_00490"
     CDS             complement(22895..23392)
                     /locus_tag="PLJ64_00490"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNC92001.1"
                     /translation="MSELTEMHKTLVTTPSDTEIRTERIFDAPPEVIFDFWTDPELLA
                     QWVGPDRLEMTVEEYDVRPGGKYRYVHKDPESGAEYVFFGEFLEIEEPRKLSQTFNFV
                     MEPQPPPSIDRLELIPCEGGRTRLVTLTTFEAREYRDGMLQSGMEKGMDEGYAKLDRL
                     LADRT"
     gene            complement(23389..23751)
                     /locus_tag="PLJ64_00495"
     CDS             complement(23389..23751)
                     /locus_tag="PLJ64_00495"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNC92002.1"
                     /translation="MVQYLDERAGSAERAFAALADPVRLAVLDRLGEGGASISELAGP
                     AGMTLTGMKKHIRILEEAGLVRTEKRGRTRWCELATEGFDEAAFWLDEFRRRRHQQLN
                     RLEEAIEEKKEQKKEQEK"
     gene            complement(24069..26411)
                     /locus_tag="PLJ64_00500"
     CDS             complement(24069..26411)
                     /locus_tag="PLJ64_00500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC92003.1"
                     /translation="MRIALVTPYSWTYQGGVNRHVQSLAEEYISRGHYVRVIAPFDPP
                     DRLSKVLHRAPAEDREIPDYLIPVGRTFGVNANGAVSNIPVFPSGVTQTRRAIEEGNF
                     DVVHLHEPTTPANGWDTCLSSDVPVVGTFHAYSTKPVPNYLANAVGARRTLNRLSARI
                     AVSQAAAWTGRRWYGGNYTIVPNGVDVDAAPDGPKPETDHLRALFVGRPDERKGLPVL
                     LRAFSALVDHVPARLSVVGAGPEDVQRILAHPELADHLDLHGRVSNEELWQQLHDADV
                     LVAPSLSGESFGMILTEAFAVGTPVIASGIAGYSDVVTHNHDGILVPPADPQALAEEL
                     QWAHHEPERLAEMGEAARESAQRYAWSNVADQVMDVYERAIEVPEPEDTVDRVRRRIG
                     IAPSDGLPWAPAVKLPSLDPVPASGPSGGGKSWLRKAGLAAMALFGLGLTAIAAQKIG
                     VDQVADKLVDSDISWVLIALALMASSLFFRAWSWFFIARSAMPHSGLKRRDFASATMI
                     GVLMSATLPARLGEPARAISLARHTGRAKDTLPVVIGTIVSQTMLNILAILLLGAFVL
                     TSMDDVFHGATKQILLYSMIPAALLLAVIAAPTLLGAQNGKGRLARIGSAIHSVMLQV
                     RRGLTVFRHPRNGGPAVALQLFAWVIQMLSCWALMFALGLESQAGLAAAAAVLLAVNV
                     TAIVPATPSNIGVFQLAVIFVLHKGWGVSTADALAYGVILQAVEMATAAALGVPALVR
                     EGLTWSDLRTQAIASAPVELDPYSKNARRQEKRRQITYTR"
     gene            complement(26454..28460)
                     /locus_tag="PLJ64_00505"
     CDS             complement(26454..28460)
                     /locus_tag="PLJ64_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92004.1"
                     /translation="MPFLKIPGRATGPANSEVRRVLSRTRAVTFGLLLGLVFSLVRVG
                     AAGADDLQVADAATGHRGQVTIIVLPPGTSPADLSGLDNAAIGLMNPGLGDVPAQQTW
                     IDVSQGARAFDSSYDTPLDRVYPGGEFAHGWEKVLKRARSTDSKVIPGLMATVLSQND
                     LDAVAADSAGAAALAVARRDGGIARAPQACGGPASMLNADSPGAKSAIRGCPDPVTVL
                     SMPLDSAFEAAANRNPRDMQIFIERPPADSGDQLSIAIAGPGMDGELRSPSTRTDGYV
                     LSTDIAPTILRHFDIKAPKAMTGLPIESGGELDIDRLADLESRYEEVGKRRGAALAIP
                     LLLWIGLAALVSFGSRGLYSRPALQLLCLATVLLPVVLLLTAALEPTLAIERAIATLL
                     PVLIAALLMRIAPGWQSLALACGLTVIPYGIDMLAGSALTPRAVIGPNPGLGARFYGI
                     GNELESTLMILTSIGTGAALTRARAGGRRAAISFLVAGVVATVVFAAGRFGADVGAAI
                     VFPVAAGVGAAFAVGRPKLAWAGLAAAFAALLLVALADIVSGSETHFVRSVLGGSGGS
                     FFEVVGHRLDATLESFTKVSRIPVTLAALAVIAIGIWKREIVLAWFEDSPMMKAGFAA
                     AAAGSLIGALTNDSGALFIQVGTLYLALVAGFAWTTSNYHPESG"
     gene            28459..29382
                     /locus_tag="PLJ64_00510"
     CDS             28459..29382
                     /locus_tag="PLJ64_00510"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNC92005.1"
                     /translation="MATETIGPEAPVLPDVQHAQVGEVEIAFDVHGDPNDPVLLVIPG
                     LGTQMVYWDMPLVEMLTDRGFRVVRIDNRDNGASTVLHHKGKPALPAMMFGIPRDLAY
                     TLNDMANDAAGVLDHLGVDAAHVIGYSMGGMISQTMAIDHPDRVLSLTSMMSRTGARL
                     DAFPGVKELAALMRTRPTDSLDSYLTGMEALAAIIGSPDFPTNKERLRNIATVAYHRG
                     IHLEGTARQLHAINCQPKRDRALSRLDIPTVVLHGSADKLVFPRGGRATARAIPGAEL
                     RIYEGMGHDIPEQLWPRFVEEIDLVARRAGK"
     gene            29431..30861
                     /locus_tag="PLJ64_00515"
     CDS             29431..30861
                     /locus_tag="PLJ64_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC92006.1"
                     /translation="MSNPGTPENPLRVAIIGAGPSGFYAADQIIKDPETHAQVDLFDR
                     LPTPYGLVRGGVAPDHPKIKSVTRVYEKTAAKEGFRFFGNVEVGRDLNVEDFEQHYHA
                     IIYTFGAEADRELGIPGEDLPGSYAATAFVGWYNAHPDYADRNFKLDQAKRAVVIGNG
                     NVALDVARMLALDDAELRKTDIADHAIELLDASTIEEIVVLGRRGPAQAAYTNPEVKE
                     LGELEQADVIVSPEEIELDPASKAWIESDEADKTARVNVEIVQEYATRQPTGKPKKVA
                     LRFLSSPVEIKGGDRVESIVIGRNELVDVDGNLKARDTGEREELACDLILRSVGYTGV
                     PLRGVPFDERRGTILNEDGRVLESHGGDHRTGHYTAGWIKRGPSGVIGTNKKCATETV
                     AHVFEDVAAGRLLDPASPDPNSIEELLRERGTHYVSFSGWEKIDAAEVGKGEPHGRPR
                     VKFVRIEEMLETAAEQAEAGAATGGE"
     gene            30861..31091
                     /locus_tag="PLJ64_00520"
     CDS             30861..31091
                     /locus_tag="PLJ64_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HNC92007.1"
                     /translation="MAADLAELKGMIEAALPGSSAEVIDETGTGDHLRAEVSAPQFEG
                     QSRINQHRMVKAAVRERFDDGTIHALTIKILD"
     gene            complement(31148..32311)
                     /gene="hutI"
                     /locus_tag="PLJ64_00525"
     CDS             complement(31148..32311)
                     /gene="hutI"
                     /locus_tag="PLJ64_00525"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HNC92008.1"
                     /translation="MSSLAIENIGLLLTNDPQLGSGMLGAVENTSITFEEGVVAEIGP
                     AGSAQADRHVDAGGRCVMPGFVDSHTHLVFAGDRSREFAARMAGEPYSAGGIRVTVEA
                     TRAAGRDELAGLTDRRRREAIRAGITHTEIKSGYGLDIPSELRCLEVAAGFTDDVTFL
                     GAHVVPAGFEDRADDYVELVCGEMLAACAPHARWIDVFCEVGAFDREQSRRVLEAGRE
                     AGLGLRVHGNQLGPGPGVQLAVEMGAASVDHCTYLEDADLDALASGSTVATFLPATDF
                     STRQPYPDARRAIDAGVEVAIATNTNPGSSNTTAMGFCLALAVRDMGMTIEEAIRAAT
                     LGGAAALGRTDIGRLAPGARADAILLEAASHADFVYRPGVPLVGSTFIGGQQQ"
     gene            complement(32308..33687)
                     /locus_tag="PLJ64_00530"
     CDS             complement(32308..33687)
                     /locus_tag="PLJ64_00530"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF006681.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HNC92009.1"
                     /translation="MSPEPAIELWCEYAWLGGEQAAASVTIGIEAGRIASVEPGTDPP
                     GEAERLPGLTVPGFANVHSHAFQRILRGRTHSGHGDFWTWRRQMYAALDGLDPDRYLA
                     LATATFAEMAEAGITVVGEFHYLHHAPGGGRYDDPNAMGRAILEAARRAGIRITLLDT
                     CYLHGGIDTTPDEAQIRFSDGDADAWAERVELIDGEVAASHGQENGGIPMARVGAAIH
                     SVRAVDPGSAARVAEWASAREAPLHAHVSEQPAENEACQAAYGATPSALLEAAGATGP
                     RFTAVHATHLDEGDFARLGGGGVCLCPTTERDLADGIGPARRLLDGGASISIGTDSNA
                     VIDPIEETRAVELDQRLATGQRGVLGCGPLMDIASAAGYRQLGWAEGGRIAPGAPADL
                     VTVGLDSVRMAGTAPADPVGSLLFAANAGDVQRVMVAGRTIVAEGRHRQIDVPAELAA
                     SIEAVEGKS"
     gene            complement(33684..34262)
                     /gene="def"
                     /locus_tag="PLJ64_00535"
     CDS             complement(33684..34262)
                     /gene="def"
                     /locus_tag="PLJ64_00535"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNC92010.1"
                     /translation="MSVREITVIGNPVLREPTRPVDPEELAGPEVQQLIDDLIDTRRA
                     ANGAGLAANQIGSNLRVAVAEVEGVNPRYPYKPPIPRLVMVNPVVEPIGEEMVEINEG
                     CLSVPDLRGNVLRHVEVRVRYLDRDGVEHDEVKRGLTAGTVQHEVDHLDGRLFLDRLT
                     DTASLATWDEFERHQRDEFVERITRFVERVGS"
     gene            complement(34259..35938)
                     /gene="hutU"
                     /locus_tag="PLJ64_00540"
     CDS             complement(34259..35938)
                     /gene="hutU"
                     /locus_tag="PLJ64_00540"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF003820.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HNC92011.1"
                     /translation="MSDGLPGARAVRAPRGNELSCKGWQQEAPLRMLMNNLDPEVAES
                     PDDLVVYGGTGRAARSWQAFDAIVASLRKLDDDQTLLVQSGKPVGVFRTHEWSPRVLI
                     ANSNLVPDWANWDEFRRLEQLGLTMYGQMTAGSWIYIGSQGIVQGTYECFAEIARRRF
                     GGSLKGTITVTAGLGGMGGAQPLAVTMNDGVALCIEIEPERIRRRLETRYLDEQADSL
                     EDAVSRCESAKAAGRALSVGLCANAADVLPELRRIGFPADIVTDQTSAHDPLVGYVPN
                     LMTPDEADALRLSDPVEYVHRSRKAMAAHCAAMVGFLDDGAEVFDYGNSLRAEAREGG
                     FERAFDYPGFVPAYIRPMFCEGKGPFRWAALSGDPADIAATDRAVLEELPGDESLARW
                     IRLAGERIAFQGLPSRICWAGYGERHRLGLRFNEMVRTGEVKGPIVIGRDHLDSGSVA
                     SPYRETESMADGSDAIADWPLLNAMVNTAAGATWVSIHHGGGVGIGRSIHAGMVCVAD
                     GTDLAAQKLERVLTADPGMGVIRHADAGYDRAIEVAGERGVAIPMRDGERG"
     gene            complement(35935..37488)
                     /gene="hutH"
                     /locus_tag="PLJ64_00545"
     CDS             complement(35935..37488)
                     /gene="hutH"
                     /locus_tag="PLJ64_00545"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HNC92012.1"
                     /translation="MSAKQTADEIRLDGKGLRFGEVMDVARFGARVALTGAAVARMEE
                     SARIVERLGNSDEPVYGVSTGFGSLATTSIPADRREQLQLSLIRSHAAGMGEPVEAEV
                     VRGLMLMRARSLAMARSGARPVVAETLLALLNSGLSPVVPEYGSLGASGDLAPLAHCA
                     LVLIGEGEVLGPGGSRIPSGEALRSAGIEPLKLRSKEGLALINGTDGILGMLVLAICD
                     LERTLRTADIAAAASVEALLGTDRAFAADLVGLRPQPGQMESAENMMKLLAGSEIVAS
                     HRQGDPRVQDAYSLRCAPQVLGAARDSVTHARRVAEVEMDSAIDNPMVLPDGRVESCG
                     NFHGAPLGIVCDLLCIASAQAGAISERRTDRLLDANRSHGLPPFLADDPGVDSGMMIA
                     QYTQAAMVADNRQLAVPASSDTVPTSAMQEDHVSMAWNAARKLRRSVANLERIVTVEL
                     LCATRGLDLRAPLAPAPGTAAALRAVRECAPGPGPDRWQSPELAAVERIVREGEVLAA
                     VEEEIGELS"
     gene            37652..38056
                     /gene="grxD"
                     /locus_tag="PLJ64_00550"
     CDS             37652..38056
                     /gene="grxD"
                     /locus_tag="PLJ64_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HNC92013.1"
                     /translation="MADQELTDRVRELINENSVLLFMKGTPEAPRCGFSMRTVQVLDQ
                     MGVEYGAIDVLPALQPLREVTSEISDWETFPQLYVNGELVGGCDIVEEMFDNGELAEA
                     LGVEQPEPAEPDFETSAPGEPYKSPPMNIEGA"
     gene            complement(38187..38558)
                     /gene="erpA"
                     /locus_tag="PLJ64_00555"
     CDS             complement(38187..38558)
                     /gene="erpA"
                     /locus_tag="PLJ64_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HNC92014.1"
                     /translation="MSEQPESAITLTEAAAEKIAELIGDSPEGEEQSLRVAVRGGGCS
                     GFQYALAFDTRGEDDHVFEHRGVSVIVDKVSMQFVFGSEVDYVEGLTGAGFQVNNPNV
                     VAACGCGSSFQVKDDAEAATV"
     gene            38659..>39509
                     /locus_tag="PLJ64_00560"
     CDS             38659..>39509
                     /locus_tag="PLJ64_00560"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNC92015.1"
                     /translation="MSAESTIALVTDSTASLTREDRERLEISVVNLYVILNGEQRPEN
                     EIDDYAAFYKAVLESPQLTTTSQPSIGDFMEVYGPLLDEGREIVSLHLSGAISGTCEA
                     ARQAAVQLEADGRGGERITVVDSRTTAGGLAFCNLAAANAIKDGKDVAGVVAQIEACR
                     EEIDTLILVDTLEYLRKGGRIGGAQAWIGGALKIKPLITLRETVEPVDKVRTLSRAMQ
                     RLKDHASEKAASGQEITWTAQHIQAPELAAELAAHCREVFQCEGAFLDEMSTVLGCHA
                     GPGLVAIG"
BASE COUNT         6872 a        13248 c        12986 g         6403 t
ORIGIN      
        1 ggtcagcccg gaagtgatga gcagattgcc gctggcgatg taggtcctca catccccgaa
       61 accgagctgt ccggccagtt tccgcagatc ggccatcggc agcttgccgg tgccgccgac
      121 gttcacggcc ctgagcagcg cgacgtatga aatggccccc accatggtcc taagactagg
      181 gcaacgcgaa atcccgaatc ctgatcggat ttagctgatc ggtatcctcg cttgatggcc
      241 gaacgtgact atctgagcgc aatttccgac cacatcgtcg tcttcgacgg cgggatgggc
      301 gcgaccctgg agatgttcga cctgacccag gaggattacg gcggcctggc cggcaaatgt
      361 cacgaggccc tggtcctgaa ccggcccgac gtgatcgaag gggttcacac ctcgatgatc
      421 gaagccggcg ccgaggtggt cgagaccgac accttccagg cttcccggat caagctagac
      481 gagtggggtc tcgccgacca caccgaggaa atcaacgtca aggcggccga gatcgcccgc
      541 aaggcagccg gcccggaccg tttcgttgcc ggctcgatcg gaccgaccgg gcacctgccc
      601 gcctcggatg acccgaccct cgggcagatc cgcttccccg agctggtcac cgtcttcacc
      661 gaacaggccg gcggcctggt caagggcggc gccgacctga tcatcatcga aaccgcccag
      721 gacatcctcg aggtcaaggc ggcgatcttc ggagcccgcg ccgccttcga ggagatcggc
      781 cgcagcgtcc cgatccaggt ttccgtctcg cttctgccca acggcggcaa gatgctgctc
      841 ggcaccgaca tccaggcggt gctgaccacg ctcgaagccc tcaaggtcga cgtgatcggc
      901 ctcaactgct cgaccggtcc cgaggacatg cgggatgcga tccgctacct cggcgagaac
      961 tcggcggttc cggttcactg catccccaac gccggcctgc ccctgcaggg cccggacggc
     1021 gagacgatct tccccgaaaa gccggaaccg ctggccgcga cgctgggcga gttcgtcgag
     1081 cgctacggag tcggcgtggt cggcggctgc tgcggcacca cgccggagca catccgggcg
     1141 atcagcgagc gggtcaagga cctgaagccg ggcccgcggc ccgagccggg cgagatcagg
     1201 gtttcctcga tgatgacttc caccgcgctg gtccaggaac cgaccccgac catggtcggg
     1261 gagcgggtca actcccaggg ctcccgcaag gcgaaagagc tgctcctggc cgacgattac
     1321 gacggactgc tccaggtcgc cgaggaccag gtcgaaggcg gagcccacgt gctcgacgtc
     1381 tgcgtcgcgc tgaccgaacg ccaggacgag gacgagcaga tgcggcaggt cgtcaagcgg
     1441 atctcgctga cccagccttc accgatccag gtcgactcga ccgagccgga cgtgatcagg
     1501 accgccctgg agcagatccc gggccgggcg atcgtgaact ccgtgaacct cgaagccggc
     1561 cgcgacaagc tcgacaccgt ggccccgctg gccaaggccc acggcgcctg cctgatcgcc
     1621 ctgaccatcg acgaggtcgg catggccaag accgccgatc gcaaggtcga gatcgcccga
     1681 cggatcaccg agatggtttg cgatgagcac ggcctcgacc gggaagccct gatcttcgac
     1741 gacctcacct tcaccctgac cacgggtgac gaggagtggc gcccgtccgc gatcgagacg
     1801 atcgagggca tccgccggat caaggcggag atccccggag tcaagacctc gctcggcgtc
     1861 tcgaacgtga gcttcggcgt cggccagccc gcccgcgcgg tgctcaactc ggtcttcctc
     1921 catcactgcg tcgaggccgg cctcgacctc gcgatggtca acccgaatca catcaagccg
     1981 tacggcgaga tcccggccga ggagatcgag ctgaccgagg acctggtctt caaccggcgc
     2041 gaggacgcgc tggaacggtt catcgcccac ttcgagtcga agggcgacga agccgaggag
     2101 gaagccgagg acgcgaccgc ggggatggag ccggaagaag cgctccactt catgatcctg
     2161 cgccgcaaga aggaaggcgt cgaagactgg atcgacaagt cggtcgagaa gatcggcgcg
     2221 gtcccgaccc tgaacgacgt gctgttgccg gcgatgaagg aggtcggcga caagttcggg
     2281 gccggcgagc tgatcctgcc cttcgtgctg cagtcggccg aggtgatgaa gaaggctgtt
     2341 tcgcagctcg agaattacct cgaccggatc gaaggccaca ccaagggcaa ggtcgtgatc
     2401 gcgaccgtct tcggtgacgt tcatgacatc ggcaagtcgc tggtcaacac gatcctgacc
     2461 aacaacggct acacggtgat cgacctcggc aaacaggttc cggtggacac gatcatcgat
     2521 gccgcgatcg agcacgaggc cgacgcgatc ggcctctccg ccctgctcgt ctcgacctcg
     2581 aagcagatgc cgatcgccgt ccaggaactc caccagcgcg ggctcgacta cccggtgctg
     2641 attggcgggg ccgcgattaa ccgcgacttc ggccgccgca cgctctaccc gcacggcaag
     2701 gagtccgatg aggtctacga gccgggcgtc atctactgca aggacgcctt ccagggcctc
     2761 gacacgatgg acgccctgat cgacgacgag gcccgcgcgg cgctgatcga tcgcctcaag
     2821 caggaggccg aggagtaccg caacaagccg gtcatcgtcg aggacctgcc gccgctggac
     2881 gatgattcgg tccgctcttc ggcaagcacc gacgcgccga tcccgtcgcc gccctggtgg
     2941 ggcgtgcggg aggtaccggt cgatttcgac gaggtcttcg accacctcga cctgcatgtg
     3001 ctcttcaagc tccactgggg cggcaagggc gtcaagggcg aggagtggga caagctcgta
     3061 tgcgaggagt tccggccgag gctggagcgg atgtggcggg aacaggacta ccttcacccg
     3121 aaggcccggc tcggttactt cccctgcaac gcggacggaa acgagctggt catcttcgac
     3181 ccggaggacc aggacaggga gatcaaccgc ctcgtcttcc cccgccagcc caagcacgac
     3241 cggatctgcc tggctgactt ctaccggccg ctgaacgacg aagggatccg cgacgtggtc
     3301 gccctgcagg gcgtgacggt cggccccgag gtgaccgagc tgatggagca actcgagaag
     3361 gacggcgagt tcgccgagca gctctacacc cacggtctcg gagtccagtc ggcggagggg
     3421 ctggccgaat ggctccacta caaggtgcgc caggacctcg gcatcaagtc gagccagggc
     3481 cgccgctact catggggata cccggcctgc ccggaccagt ccgaacacga gaaggtctgg
     3541 gaactgctcg acctgggcga gatcggcatg tacctctcgg gcggctacgc cgtgatgccc
     3601 gaacagtcca cggtcgcgat catcgcccac catccgcagg cggtctattt cggcatgaag
     3661 tccggtttca tccccaaggg agaagccccg gacgaggtca tcgccgaatc cgaccgctcg
     3721 gccaatccgc ccgaggccga tccggacgaa ggcccgaccg aagccgaggg cgaaccggct
     3781 accgcctgaa gcggaaggtc ttgacctttc gtacggtctt gcccgtttcg atgtctaccg
     3841 ccgagaagtt cgccaccagc ctgcatctgg tgatgttctg gcggatgtag ctgcggaaag
     3901 acggactgag gatcacgccg atcgtccagg tgcccgtggc ctcgaggccg atcgatccgc
     3961 cgccgcggac ggtggtcacc ggggtgacca gccggagctt gacccgcgcg ttgcagtcct
     4021 tcgagcagga agcgatcagg cgaaccttcc gtttgacctt gagccgctgc ttgccgtcaa
     4081 aggtgaccag cttcaccttg agcggcttcg ggctgccttg aagtgcggct ccggcgggtg
     4141 acccgccggc gagcaaggcg gctgaggcca gggcggccac aagaaacaag ggcgctttct
     4201 tcatcgattc ctcccggcgg ttgagaactg ttcccagcct aaggcaccag accgcacccg
     4261 ttcattccgt acggtgttca taaagtagga tcagctcaac cgaccaaggg agtttggatg
     4321 ttcgccaggg gtttcctagt ttcgcttttc gctgcgttcg cattgatggt tccaactgct
     4381 gccgcttcgt ctgcgcctca cctgagcttg cccgcggggg actcgaccga caatggcaac
     4441 gtctacctcg gtgattccag tgtgatcacc gtgacggtga attcagacgg atccagtccc
     4501 ctcgacatca cttcggtctc gatttacgcg ttcccgggga actccgaata tttctcgatc
     4561 gagcaggaca actgctcggg tcaatcggtg gagtccggcg accactgcac cttccaggtc
     4621 aggttcaccg cttccgtggt cggccacaag ttcacttcgg tcaggatcga cagcaacgac
     4681 gtggaaagtg gcggcatgaa gttctttggc atcagcggta ccggcatcaa cccagggatc
     4741 gacgcggatg ccgcgagtca caacttcggg acggtccagg ttggtgagta cggaactgac
     4801 aagaccttca ccatcgagaa cgacggaacc tcggacctga acgccaccgc gaccatcacc
     4861 ggtaccgatg ctgccgagtt cttcttcagt tcccagccca actgcgcctc ggtcacgccg
     4921 gattccacct gccagatcat cgtgaatctt cgcccgctcg ggagcgccgg cccccgctcc
     4981 gccaacctgg tgatcgccaa caacacgaag aacgagaacc cggtcacgat cccgctgacc
     5041 gccaccgcca gcgcccctca tttcaccagc gagcctctgg gactggggtt cggtcagttc
     5101 gacctgttcg atgacccgcc cgcgaccagg cccctgacca tcaagtccga cggaaccgcg
     5161 gacctgaaga tcaacggcat ggtcatcgac cccctcaact cgaactccaa tgccttcacc
     5221 ctcgaccagg gggaatgcgt ggacgacctg gcgatcgcac ccggtgatga ctgcacgctc
     5281 aacatcactt tcaaggcagg ttcattcggc gagggctcct acaacaccag cttgctggtc
     5341 ttcacgaaca acggcgacca gtcggttccg gtcagtgccg cccggacctc cggcggcatg
     5401 tccaccgact catcggcaga cgtcggggac gtggagctcg gccagaccgg atcggcgacc
     5461 ctgaccctca cttcgaccgg aaccgcgccg ctgaagatgt caccgcccgt aatcgacggc
     5521 cccgacgcgg gcatgttctc ggtcgaccag ccgagcgact gcaacggtct cgccagcggc
     5581 cagagctgcg aggtcaccgt ccacttcacc cccacctcgc tggccggcaa gcaggccggc
     5641 ctgaccctct cgggcaactt cggcagcacg ggagtgctcc tgaccggctc cggcacccag
     5701 gccttcgtca acgtcccggg tgacatcacc ttcccgacaa cgatcgtggg caagcaggtg
     5761 acctgggacg agtccctgat ttccaacggt tcggccccat tcagcctcgg ctcattgacc
     5821 atcgagggag ccgacgcgtc gatgttctcg gttgagacaa ccggcagttg cgcgagcgtc
     5881 gcccacggcg atgaatgccc gtacagcatc acctacaagc cgacctcgac cggaagccac
     5941 gaagcgcagc tgaagatcac cggaaacctg gcagggggat cccggacgat cccgctctcc
     6001 ggcaccgcca ggaacctgac tccggcgaag gtctcgttga agttgaccgg agccaaacaa
     6061 gtgaagcccg gaaagaccgt gaccctgacc gccaggatct caaagagtgg cgaggagcag
     6121 accggagacc tgaccctcaa gacggtggtt ccgaagaaga tggccgcctc ggtgaaaccg
     6181 gtcaggatcc cccacctcgg agcaggcaac cggaccgtca ccaggaagat cacgatcaag
     6241 gtcaggagga gcgcgaagag cggccagaag ctgaaggtcg aggcgaccgt gtccggcggc
     6301 ggcctgaata aggccggcaa ggccgggaag accgtgacga tcaggtagcc gagtcaccct
     6361 caagcacggt gcggatgctg tccggaattg gctggggagt gtgttcggtt gacgagacgc
     6421 agacgtagga gatctcgatc tcggcgatcg cctcgccgct ccgggtgaat tcgaactcga
     6481 tcgtcatcga gcgcgcggtc atgccgccca cgctcacccg gatatcgaac tcgtcgtcaa
     6541 actcgagcgg ctgccggaag cggaggttcg cctcggccag gaccatgtcg aggccgatct
     6601 cgttgaactc cgaataacca cccaggtgct cgcgccagaa ctcggtaacc gccacgtcgg
     6661 tgtagacgag gtagttcgag ttgaagacga tcccgacccg gtcgcactcg ttgtaacgga
     6721 ccctcagcct gtgggtggtt tcagcggcca tcagctcccg cgagagtact tcgttgcgca
     6781 tccgtccgac cggccggggc gaccgtctcg ttcatgaatt cgacgatcag gttcgagacc
     6841 ttctcggggt cgtcgagggc gacgaaggtc ctgccgcctt cgaccttctc gatgcgggag
     6901 ttcgggatca tcgcggccag ccgctcggcc agttccggct tgaagaaacg gtcgctttca
     6961 ccccaggcga agagcaccgg cttctcgaat ccccgtagct tctcggcggc gtcgatcgcc
     7021 tggttctttt ccagcccgcc gatcaccttg tttacgtccc ggcggattcc gggattgctt
     7081 ccggtcgggc ccagccacgc cttcagctgg cgatccggga tcggatcggc ggtcagcatc
     7141 cggtaggtgg cccagcgcac cggcctgagc ttcatcagca ggccgagcgg ggcgattcca
     7201 ccaggcatcc gggcgagcgg cggaagcagc gtgaagggaa acggcggata gacctcgagt
     7261 gcatcgcagt tggtgagcac catcgccgcc aacttgtcgg gatgctgcgc ggcaaacatc
     7321 tgactgacgg caccgccgga atcgctgcca accacggtca ccggaccgag atcgagcgca
     7381 tcgatgaaat cagcgagcag gtccgccacg cggggcgggg agagaggcgt ctcggggttg
     7441 agcggaatcc ggtgggcacc catcggagtc tccggccgca cgcagcgaaa cccggccgga
     7501 accaggcgct cgaccagccc gtcccagagc gatgagtcga cgaagatccc gtgcgagaag
     7561 aggagcaccg gcccctcccc ctcgtcccgg aatgcgaccg tgccctgggg caggtcgacg
     7621 gtcagtttct tttcttggtc ttgccgggtc atcggtcctc ctcaggttgc gtaccgccgg
     7681 tatgtattgc cagcaagata acatacccgc ggtatgcaag ctgcgagaac ccagatcgag
     7741 cgctcggacg caacccgccg cgccctgatc gattcggccc ggaacctgtt cggatcgagg
     7801 ggctacgcgg cggtgcccct ggacgaggtc gtctccgcgg ccggggtcac caagggagcc
     7861 ctctaccacc acttcccggg cggcaagaaa gacctgatgg aggcgcttta cgaaacggtc
     7921 gaagccgatt tcacggagcg ggtcaccgcc ggcatcgcgc ctcgactggc cgaaggagag
     7981 gttgacccgc tggacatcat gggagcggcc attgaagaga ccctcgacct cagcatggat
     8041 cccgagctcc agcagatcct cctgctcgat tcgctagccg tcctcggctg gagtcgctgg
     8101 cgggaaatcg cggacaagta cagcctcgcc gtggtcaagt ccctcctcgc cgccgcggtg
     8161 gaacacggct cgatgaaacc gctcccgacc gaatcgctgg ccaacctgct catggccgcc
     8221 ctttccgaag cgatcctgat gatcccccgg gccggggacc aggagcaagc gaaagccgac
     8281 gcatccagat cactcaaggc gatcatcgac ggcctgcggg cctgaccgat tcggtgtaag
     8341 acttccgggc atgagcgaat tcacggcttc ctatcgtggt ggtgacggtc cgactctggt
     8401 gctgattcat ggcttcacgg gcacctggga aatctgggat ccgatcatcg ctccgcttga
     8461 gcagcactac gacgtgttca tcccgaccct tccgtcacat gcgggcggac cggcgatcga
     8521 aggcgacccg accctggagt tgctgctcga cggagccgaa gcgctgctgg acgaggcagg
     8581 aatcgagacc gcccacatcg tcggcaactc acttggcggc tacgcggcgc tccggctggc
     8641 ggcccggggc cgggcgaaga ccgtcaacgc attggctccg gccggtggct gggccgaagg
     8701 cgacccggtg atcgcccaga ccatggactt cttccagcag atgcacccgc tggtcgttgc
     8761 cgcagccgaa cacgtcgacg acgccgtcgc caccccggaa ggccgggcga tgctgctgca
     8821 gttcctgacc gagcatcccg aagtcgtgtc acctgatctt gcgaagaaga tcctgctcgg
     8881 cggggccggc tgcaccgagg cgatcgccct gaccgagatg ggccgcaccg cggattgggc
     8941 gtttgacctg aatgccatcg agtgcccggc caggatcgtc tggggcaccg aggacaaggt
     9001 gctgggctgg ccgatcgccg cggcccgcta ccaggaggga cttccggatg ccgactgggt
     9061 gatcctcgaa gggatcggcc actgcccgat gctcgacgtg cccgagcgga cggtcgagct
     9121 gatcaccgaa ttcgcgaagg actgacccgg cccgcctggc cgggcagatt tagcggctca
     9181 gaagccgcgg cggtttcgga gccgggcgcg gagggcgagg gtggcgccag tgagggccgg
     9241 ggcgagggcg aggagttctt cgaccgggag cgggccgatg tgggcgagga tcatgagccg
     9301 tactcgtcgt ggcggcgcca ccactggtac ggcggggtct gcggataacc ctcgggcgaa
     9361 tcctcccact cttcctggcg gccgagcgcg gtcaggtcga ggtgggccca ggtgctgccc
     9421 atgtgctcgt cgccgcgcgc gtcgatgaag taggtgcgat agaccgtgcc gtcgtcatca
     9481 cggatgaagg cattggttcc gtgccactcg ccgacaccca tgtcgcggtc gaagtcgtcg
     9541 atcaccgtgt accaggggat ctgctcccag cccatcttct ccttcagccg ccggatgttc
     9601 tcctggctgc cccgggaagc atgggcgaag gtcgtgtccc gggcattgag atgccggagg
     9661 tcggcgacct gatcggcccc gaacgagcag ccagggcaac cggcatcggg gtggccgctt
     9721 acatccgggt cgaagaagaa gcggtagagc acaagctggc ggcggccttc aaaaaggtcg
     9781 atcagcccga ccctgccctc ggggccttcg aactcgtagt ccttctcgat cttcagccag
     9841 ggcatccgcc ggcgttcggc ggccagcgcg tccctggccc tggtcacttc cttctcgcgg
     9901 accagcaact ccatccgggc cgcatcccat tcctcggccg agacgaccgg tggcgtcttc
     9961 atctgctcga acatgactac tcatctcctt ctgcttcgaa atgccgcccg agactcgcca
    10021 gagcctgggt ccagcctgtg ctgactcctg tgggtccgcc cggcggcaca tcaccaaggc
    10081 ggtcgtgggt cagcgaaacc tcggtcgctc ccggcccggc ttcccgcagc tcgatcgtca
    10141 ggcgggagtg gtgaccatcg gggtcggcgc cggccgggcc gaactgccag tccatcacca
    10201 gacgccggcc gggaaccacc tccaggtact gccagttgaa accgcccacc tcttccccgt
    10261 ccagggcgtg gatcagctcg ccccggccgc cgacccgggg gtcgttgctg gccgagactc
    10321 cggtgaaggc atcggggtgc atccagcgga ccagttcatc cgggtcgatg aacgcccggt
    10381 agaccatttc ggggggagcc tgaacgacct tgacgagatg aacggattga tcagagacag
    10441 tcatttcttc tccttcttca tatccgcttc cactacccgg gcgaggcgat ccagattgcc
    10501 tgcccagaac ttctcgtaga agctcagcca ctcgctggcc tccctgagcg ggtcgggcct
    10561 aagcgaacag atgtgctgac ggccttcaac cctgcgattg accatccccg ccttctccag
    10621 aacctgaacg tgtttggagg ccgcggcaaa ggacatccgg gtcggccggg cgaggtcgcc
    10681 gaccgaacat tcgccgccgg aaagctgcct caccatcccc ctccgggtct ggttcgagag
    10741 ggcgtgaaac acccggtcga cttccttctg atcttcaacc atgtggttga acgtagagca
    10801 cggacggaag attgtcaacc ggttggttga ataaaagttc tcgcggactg cccgcccctt
    10861 cgcatcgaac ggacccggac agtgttcggt acggtgagat caaccgaccg atccgagggg
    10921 gattgtcttg cgcaagctag tgacgttcgt ggccgttttg ctcggcctgc tcgccgtacc
    10981 ggccacagct tcggccctga agttcgaggc ggctcccggc acgccgctgg caccggcgac
    11041 ctcggtcatc gacccttccc cggtcatgcc ccctggcttc tccccgaccg acatcgcgac
    11101 cggggacttc aacaaggacg gctacggcga catcgccgtg atcacggact acatctccgt
    11161 ccaggggccc gacggaccgg aaatcagccc cctctccaag aacatctacg tttacctcgg
    11221 cagcgcggat ggcagtctcg agtcatcggc aagcaatccg atcggtgcgg attcgccgat
    11281 cgcgggcggg cgtttcatga tccgggcgat cgatgtagat ggcgatgatg acctggatct
    11341 ggtcaccggg tccgggggca acccgaccat catcgagatc taccagaacg gcttcaacgg
    11401 cactcacgag gcgtttgcca ccgttcccgg ccagacgctc agcctggatg acagcgagtc
    11461 cgccgccggc taccggtcgg tttcgcttgg ggatcaggac gacgacggtg acgtcgacat
    11521 ggctctcggc ctctacgaca accgttacgt cttcatccgc aacctgtcgg gtctggacgg
    11581 cctcgagatc ccgaacccga gcgtcgaggt ttcgcgcgac gacccgcagg acatcgacgg
    11641 gatcacctcc accgcgatcg gcaacttcgg cgacggcacc ggcccggacg ctttcggcga
    11701 cctggtgatg gtctccagcc ccgggttccg ggccgaaaac cagcccccgg accggctcct
    11761 ttacgcaagg ggcgatggcg catcgagctt cgcctcgccg gttgaactgc tgcgaaccgg
    11821 aaccgacgaa tacctgggct gggtgagcaa ggccgatctg aacggtgacc agtacgacga
    11881 catcgtcttc aaggtgcaga ccgccaccgg agaaaacatc gtcagggccg cgctcggcag
    11941 cgccgacgga ccggtcatcc aggccggcga aggcagctcg atttccgtct acggcgccca
    12001 gcagccggcc ccgggagact tcaacggtga cggtgaccag gacgtagcga tcccccagtt
    12061 cgtcgatgac ggattccagg ttggtctcgg cgacggcagc ggcaggctgg ctctcgactt
    12121 cgccggaccg ttcgccctgg cggcaatcga ctcggctgac ttctttcccc aggcaagcgc
    12181 caccctggac gccaacgggg acggccggct cgacttcgcg gcggccagcg gccattcggg
    12241 tgatgcggat caggctcgcg gcgtcgcggt catgctgaac aagccctcct ccgggatctc
    12301 cgtctcaccg acttcgatcc acttcggaga tgtcccgttt gacgccgagc tgatcgcgcc
    12361 ggttccggtc acgatcaaat ccaacggtga tctcccgctg aacctcggtc agatcggctt
    12421 cagcggccct gcgaccggag gtttcagcct cgaccgcggc aactgcccga acggcaacct
    12481 cgcgtccggc tcgacctgca cgctcgaggt ctcgatgaag cagaaccagt ccggctactt
    12541 caacggcttc ctcgacatca ccagcgacgc gaccggcgat ccggtccgga tcgaggtgat
    12601 cggccaggtc ggggaagccc cgcccactgc cagggggctt tccctgaagg tgaagtcggc
    12661 gaagaaggtc aaggccggaa agaagctggt cgtgtccgcg atcgtccgca acagcggcaa
    12721 ggcgcgctcg gcgcatttca cgatcagggc caccgcaccg aagaagatga ccggaaggat
    12781 caagttctcg ccgcttcgca atctctcgct cgacggtcgc agctacacga ccttcttctt
    12841 caaggttccc gtcaagaagc aggcgaaggg cgtgctccgg gtcaaggtga gcctggtggc
    12901 aaagaaccgg aagatcgtgc gaaagacagg cgaggtcagg atcgtgaaga aggcgcggcg
    12961 gcggtagagc cttcgcctgc gttcatttca tccgcttcgc caggcggatc acggccctgg
    13021 cgtggcggtt gatgaccgcg cggggctgaa ggccggggcc gagttcgacc acgaaggcgg
    13081 tggtgccggt tgcctggtgc tgccaggaga cggcgctgcc cggataccgg ccgtgatcac
    13141 agcttccccc ggtccccagg ccggagagcc gggcgtagag aaaggcccag cgtcgctggc
    13201 cgtcacaggg cgcgagggtc cggccccagg gctggtggaa ccagaccgcg aggtcgaagc
    13261 cgacccggcg gctcagtcgc atgatcgccc gggtctcggg ttcggaggcc ggtcgcgggc
    13321 ccgagttata cccgccgttc aggctgccgt cccagcggaa gggaaagttc cggttgaggt
    13381 cgatccggcg ggcgtttcga cgggtgccaa gcgcggtgcc atcggggttg gcggtcttca
    13441 cggtccagcc cccgatgccc ttcggcccgc cacgcccggc caggttctgc ctgagcctgg
    13501 ccacgacccg catcccctgc cgctcgtcgc cgtggaccga accgaccacc agaacccgga
    13561 aatcgccctt cggatcaccc tgacgccagc tcacgatcgg ccgcccgtca accgaacggc
    13621 cgatcacctt ccgctcggtc gcgccgctcg caaccggggc cggcaggatt gcggcagcca
    13681 gggcgagcgc gatcagatgg cggtgcttca ctggtcaaac ctatccccga tgactgggag
    13741 aatgtggcgg tgagcgaact cgaagatgac gggctgaccc gctgcgcctg ggccaacggc
    13801 agccagatga tgcggaccta ccacgacgag gagtggggct gcccgatcgt cggcgagcag
    13861 gacatgttcg agcggctctc gctcgaggcc ttccaggctg gtctcagctg ggcgaccatc
    13921 ctgcgcaagc gccccgcctt ccgggaggct ttccgcgact tcgacctcga atactgcgcc
    13981 ggccttaccg atgccgatat cgaggaactg atgggcaacg aggggatcat ccgggcccgg
    14041 gccaagatcg aagcgacccg gtcgaatgcg gccgccacga tcgcgctgcg cgaggagggc
    14101 gggcttgagg gcctgatcgg ttccttcgtg cccgaacgga tgcccgaacc ggcgaaccag
    14161 tcggaggttc cgaccagctc gcccgagtcg accaacctcg ccagggagct ccgcgctcat
    14221 ggcttcaggt tcgtcggccc gaccaacatg tacgcgctga tggaggcgag cggcctggtc
    14281 aacacccatc tgaccagctg cttccggcgc gatcaggtct agccgggccc cgcgaccggt
    14341 cgccagcgtg gcgagacctc gccgaccttg tcgggcggca ccatctgccg ggccgaatgc
    14401 ttctggagca ggtacatcag gacgatcacc gcgatcgaga cgaagttggt cattcgggcg
    14461 atcgacccgt cgacgtggtg gctgaggacg atcacgtcgt tggtgctgtg ggcggccatc
    14521 gccagcaccc aggccccgac tgccgccgca gtgaagatcg acttcctgtg ccaggcggcc
    14581 tgccaggcga ccgccgccac gaatccggtc agcagcggat gcatgatctc caggaccgga
    14641 cgcatcggct ggtagatctc gggctggacc gcgtattcgg tgccttccat caccgccacc
    14701 gtcgcggccg agaccagcac cagcaggtaa ccctgacggg gcagccggaa cctgccccgg
    14761 aaccagagga tcaccggcag cagcagcttg cccgtttctt cggccggccc gtccagcacc
    14821 gaccagcccg gatcatcctt gaggttgtcg ccaaagacgc gcgggaggaa gaactccacc
    14881 gcgaaggcca gcgcgaaggc caggacgccc gaagcgatgc cccacttgac gatttcccgg
    14941 cgcgtttcgg ccgggatccg gtcgaaaccg acccggcggc caatgaatct caggaaggcg
    15001 accatcgtca cgaaggtcgc cgccgcagcc aggggcacgg ccaggatctg gatgccgccg
    15061 accaaatcgg ccttgcggtc ggtaccccac aacgccgccg cgccggcaag ggtcaggatc
    15121 aggaccagca ggagcttcca gttgccgttc cggacgaagc cccagaagtg cctccggtag
    15181 gtagcgagac ttgaaccgac cggagcggtc ctgacctcgt cggtccagac ttcgccgtcc
    15241 cagtggcgct cgacggtcgt gtcccgatcg cggcccggga cggggtacca gccaggtgcg
    15301 ggaaggctct tcgcggaaga gtcggcagcc atcccacggt agctaccccg gaaccagcgg
    15361 atcaatcagc agcagccagc cgtcgatccc gatcggtgcc gtcggagcgc cgatgtgaat
    15421 cggtcgcgat ccgctcatcg tctgcggcga tcggctcagg tttccagccc ggacccttga
    15481 aggcgtagcc gaaccggtcc cgccagctgt cggcggcccg cacgtcacgc cagatgttca
    15541 tgtattcgcc gtaggcgacc ttgaccggat ggaacgtctt gatgtttttg gtcaggccgt
    15601 agcgaacccg ttcttcctcc ggttcgaagc tgccgaacat gcggtcccag atgatcagga
    15661 ttccgccgta gttcttgtcg aggtactgct cctgggagcc gtggtggacc cggtggtggc
    15721 tgggcgtgtt gaagatgaac tcgatcggcc ggggcagccg gtcgatcgcc tcggtgtgga
    15781 gcaggaactg gtagagcagg ctccatgaaa acgcgaggta gatcatccac ggcgggaaaa
    15841 gcagggccga aggcatccag aagaagatcg agctgaacgg cgaccagtcc tgacgaagcg
    15901 cggtcgagaa gttgtaatgc tcggaggagt ggtgaaccac atgcatcgcc cagaagagcc
    15961 ggatgcggtg gtggctgcgg tggaagcagt agtaggcgag atcgtcggcg aagaagagca
    16021 gcacccaggt ccaccagtcc gaaggtgaaa gctggatcgg gctgacttcg tagatcgcgg
    16081 cgtagatgac gagaacgacc agcttccagg cgccggcgat gaacaggtgg ccgaccccca
    16141 tgctgagact ggtggccgtg tccttcgctt cgaagccgat cggcgcgtcc gggtttgcgg
    16201 cctgcgcttc tccgtgctca tgcgccgcgt ggcgcagggt caggtactcc agacccatcg
    16261 caaggaagaa gaacgggatc gcgtaaagaa tcagatcgcc cattgccgaa ctggttatac
    16321 cagttcaccc agacggttgc aaggggcgga tcggccgcag ccgggatccg gttcaggcga
    16381 cgcggtcgat gtcgctgcgc agcatgtggc cagcgacctc ggccgccgtc tcggggtcgc
    16441 cggcgccgat cgcgttgccg agccgggtga tgatccgggg gtcggccggg cggatccggt
    16501 cggccacgtc tccgaaggtg agcatcgcct tgagcagcga gttgagtgaa agccggtagg
    16561 cgatattgcc ggacccggcg acgatcgcca gccagaaggc ggcgaaggct tcatccagtc
    16621 ctgcctcgtc tccggttccg atcattgcgg cggcgctttc gcccagttgt tcgacctgat
    16681 cgcgttccga ctggctcgca cggctggcgc agagcctcgc cgcgtcaacc ccgatcgagg
    16741 ctctcatctc gaccacggac ctgacgatct cggccggtgg ttcgagcggg tcgccaccga
    16801 ggtcgagcag aacctcgagc ccgccggcgt cacgccagtc cagcaccctg gtcgcaccgc
    16861 cgtgggtgat cctgatcagc ccggctgcct ggagaccctt gagcgcctcg cgaacggcat
    16921 ggcgattcac gccgaagtct tcggcgagga cccgctcgga cgggatgcga tccccgggcg
    16981 caagttcacc ctcgacgatc tggcggcgca gctcgtcgtg gacgcgacgg gaaacggtct
    17041 ctgtgccgga accgggcatt gcaggaggtt ttaccatctc cctatggccc gattgagact
    17101 gctgttcacc ggtgcgctcc tcgcgttcgg tcttcagacc ctgattcgca ggctgctgct
    17161 gacgcaattc cggcggagcg tggaggccct caaccagggg gactacggcc cgctgctgga
    17221 cggtttcgcc gatgacgccg tgcttcattt caatgacgcg ccacatcgct gggccggcga
    17281 gcatcggggg cgcgatcaga tcgagacctt cctcaaggat ttcgtagcgg ccgggctcca
    17341 gggagaaatt ctcgacctgt ggacttccgg cccaccctgg gcgatgaccg ccgtggcccg
    17401 ctttgacgac cgatcgaccg gaccggacgg tcaggagctc tactcgaacc agaccatgat
    17461 catggtccgg accagatggg gcaaggtgac tgagcagcgc gacttctacg ttgacacgac
    17521 cagcatgatc gatttcgacc gcaggctgac cgagctggga atcctgccgg tcgccgacgg
    17581 ctgagatgct tccggcatga cccccgggtt cacccccttc catcacggcg gatcgggacc
    17641 gccgctggtc ctgatccacg gtttcaccga cacctggcga acctgggatc tggtcatccc
    17701 ccggcttgaa cgcgacttcg aggtgctggc ggtgaccatg cccgggcatg ccggaggccc
    17761 ggcgatcgaa gggaagctca ccgtcgacac cctcccgtcg ggaatcgaag ccgcgatgga
    17821 agcagccggc ttcactcatg cgcacctggc cggaaactcg ctcggcggct gggtcgccct
    17881 gcagctcgcg gcgcgcggtc gcgcgctttc ggtcaccggc ctcgccccgg ccgggggctg
    17941 gtcggcggag ttccagaacc agtgggcgct cgattacttc atcacccagc aggacctggt
    18001 caagaaggcg gccccctacg ccgatcagat cgcgtcgacc ccggaaggcc ggcgccaggc
    18061 gacccgcttc atcgtctccg acatcggcct gatcacgccg gaaatggtgg ttcaccagtt
    18121 gatcggagcc gccagctgcc cggccgcgat gccgatggtc gaactggccc gcgaacaggg
    18181 cttcgagctg gacgcggagc agatcgagtg cccggtgcgg ttcgtctggg gcacggctga
    18241 cctgctgctt cgctggccgg agacagcggt ccgctttcgc gaggagtggc tgccggcggc
    18301 cgagttcatc gaactcgacg ggatcggcca ctgcccccag ctggaggtgc cggccgaggt
    18361 cgccgagctg atcgccggga ccgcccgcct ggcctgagcc gcgcgtcggt cgttcagggc
    18421 cggcgaccga agagcacggc gaagccgtgg tccttgaaga gacagtcgac gtcggtgaaa
    18481 ccgatctctt cgagccatcc aagctgggtc gaaagcgggg tcaggtggtc gtggctcatg
    18541 cgttcgacgg tgtcggccca ttcggattcg gtcaggccct tctcgaaggc ctggcggcgg
    18601 tgccattttg cgtagtgctg ctgcagggcc ggggtctcgc cgaggacgtg ctcggcgttg
    18661 acgaagatgc cgcccggtct caactccccg aaaaccgcct cgtagaccga ccgctttccc
    18721 tcgtcggtca ggtggtgaat ggcgagggcc gagatcaccg cgtcaaaggg cccggccggg
    18781 attccctcgt acatgtcccc ggtcagcacc cggaccgctt ctcccaggct ttccttcgcc
    18841 tgttccagca tccgcgggga aaagtcaaag agcgtgaacg ccgcatccgg ccaggcttcc
    18901 gcgaccaccg cggagagcat tccggttccc gcgccgaggt cgaggatcct gagcggctgg
    18961 ccctccctcc cggtttcggg ttcggccagt ccgatcgctt ccggcaccat cccgtagaag
    19021 cgctccatct ggggaatcag cttctccctt gggccgcgat aggtcgcggc ctgcttctcg
    19081 aaaaccgagg tcgccctggc catgggtctg atcctagatc ctcgaccggg tcagtcccct
    19141 tcttccacca gggggacgaa ggcgacggca accatgcgct cgcgggagag ccggccttcc
    19201 cgccttcgcc agagctccag ccactgatgt cggcgcccga ccggcaccac catccgcccg
    19261 ccctcggcga gttgccccgg gagcgcctcc ggcacctcgc cccgaactgc cgcggccacg
    19321 ttgatcgcgt caaaaggcgc ttcccccggg cagccgaccg acccgtcacc gcagatcatc
    19381 tccacgttca cgacgccggc cgcagccagg gaccggcgag ccgcttcgac gagttccggc
    19441 tggcgctcga ttgagatcac ccggcgggcg agcctcgcca gcaccgctgc gtgatagccc
    19501 gagccaccgc ccacgtcgag gacgaggtcg tcctcactca gctcgagcag ctcgcacatc
    19561 gtcgccacga ccagtggctg ggagatcgtc tggccccggc cgatcggcaa cgggcggttc
    19621 tcccaggcaa accggcgctg gtcctccgga acgaagagat cgcggggaac agcggcgatc
    19681 gcgtcgagga ccctgcggtc cccgacctcg accgccagct gttcgatcag ccgatcccgc
    19741 tcagtacccg cgactttctg ccttcgctcc cccgccgaat cgcttcacct tgagccccgg
    19801 tgacttcttc tgctggttgg ggcagaagcg atccagcgac cagcccttct tcgagaagag
    19861 cttcgtctgg tcggcgtaat gcttcgactt cgggttctcc gactgcgagt aggtcaggat
    19921 catgctcgcc cgcgggcact tcgaaccgtc caggcgaacc gcctggatga agctggagcc
    19981 gtcgctgacc tcggtgatgc ccttgcccgg aatccagggg ccccagatcg tgttgaacga
    20041 accgtagggc tcgtaatccc cgccgtggat cgggatcttc accccgttgc gggtcaccgt
    20101 ctggtacttg cggcgggtcg cggtcagcgg gatgccctga tcctggaact cggtgatcgt
    20161 gtcggccaat gcgatcggca gcgccgaatt gagcgtgttc agcccgttcg gggtggcgat
    20221 cggatcggcg atgtcgaagg gattcgcgta gaccgggtcc gtgctgttgg agtagagccg
    20281 gccggcgaac ctctggaaga acagaccgcc cttcgagtcg aggttgttgc ggccgtccca
    20341 tgcctcgagc gcgtcgcagg cacttgccgt cgacaccggc ccgccgctcc cgaccaccga
    20401 cgggttggcg ttgcagtaat cgatcaccgg gtcgagcaga agctccgcgc tcatcacccg
    20461 gtcgttgaac tccattgcct tgagcgaggc gagggtgaac ctgccgtcgc ccgcgagctt
    20521 ctcgccgacc atcttgtggc cgagccgggt ccgctcggac tgggcgatcc cttcctgacc
    20581 gatgatcagg gggaagccgt cgagggtgtg tttggagttg gtgaaccagt aggagtcgtt
    20641 cgagttctcc gtgtagtcgg agcgcacctg atacggcatc tggctgggcg gcaggattcc
    20701 gggaccggcc gcaccgggcg cctttttgct catgtcgcag ttcgaatccg aaccttcgaa
    20761 gatcggcacc cggtaggacg gccaggtcac gatgccgagg ccgacgttgc aatcggcgac
    20821 tcgtccatcc gtgacgttcg gtaccgcgcc gatgtccgcg taaagcgccc ggcccttcga
    20881 atcggaagcg atcgtgttga cccaggggat gccctggtac ttcttcagga tcttcaggac
    20941 ctcgggcacg ctccgggcct ggttcatcga gtagaagtgg ttgatcatcc ggaagttgcc
    21001 ggtgttcgcg tcatagagcg agtagccgta atccgtcgcc cagccgaaca ggttgctgcc
    21061 ctggagctgg ttggtcatgt agccgtgctc ggtcgaatag agggtcctgg tgtgaaccat
    21121 cccgtccttg tccttgaccc tgaccgtggt cgccttcatc ggcttcgact ggccgtcaac
    21181 gatgtagcgg gtcgggtcgg tcgggtcgag cgcctcccgg aagaggatga aacggcgggc
    21241 ggtcgaaacc gtgtggctcc aggccatggt gcgggtgtgg ccgatcagga tcaccggcac
    21301 cccgagcagg ctggccccgg aaacgttgag ccggccgggg atggtcagct gcgactcgaa
    21361 gaagcgctgg gcaccatgcc aggggaagtg cgggttgccg agcagaaggc cgccgccacc
    21421 cttgaccgag ttcttgccca gggcgaccgc gttcgagccg agaccgccgg tgaagttgcc
    21481 gaagcccttc gggaaatcac tggtggtgat gtcggcggga ttgacttcat cggccggggc
    21541 ggagccgttc ttcctcgtca tcgccaaggg cggctgggcc tcggcgatgc cggccatcga
    21601 ggggtcggtc gagccgtaac cgagcagttg gtagaagcgc atgtagacgt cgagcaccga
    21661 gatcggcttg acccaggcct tgccgcggca acgcgggtcg ggcagcttcc ctaccccggt
    21721 cttcctgagg taggcgttgt aaccggcggc gtatcccctg accgcctgct tgacgtcggg
    21781 cagcggcccg ttcggcggct tcaggttgat catcttctgg acggtgccct ccttcctcac
    21841 ccgcttccag aagaaatccg agtcgaggtt cgtaacgcca ttcggggcgt tgacgtcgcc
    21901 gccgaagtag agcgaccgct cgccccgaac cgtggtgtag gtgtcagcta gcgtgcatag
    21961 ctgctcgctg gcgatcgccc agccgtagcc gtagccgagc ccgtaggggt tgtcggcgag
    22021 gatccgcggg atcccgtact gggtggtgcg gatcgtgacc cgcggcggct tcttcgccgc
    22081 gtcggcccgg cccgccagcg gcatggcgaa aaggagcatc agtaccgcgc caagcccgaa
    22141 cagaaccccg aacaaacggc ccaggggacg cattgccccg gacgaagaca cctgcatcat
    22201 tgactctcct tcggttgttt ccctcgcgaa aagactaact gcagtcggtc cggtctgtgc
    22261 ggaccccggc gagagcccgc cctcccgttt gaagccctga atggctgggg gcggctccgt
    22321 tcggactaga gttggatcat ggagagctca gcgcaggatc aaactttgaa accggccgaa
    22381 cacatgaccg gcgagtgcat gtgcggcaag gtgaagttcg aggtcgacca gcccctgatc
    22441 ggggcggccc tctgctactg caaacgctgc cagcgccgca ccggatcggg cgtctcggcc
    22501 accggactga cccagccggg ctcgttccgg atcactgaag gcgaggattc cgtcacgagt
    22561 tacgagcccg gcgacggcgg cttcaacaag tacttctgcc gcaactgcag cggccagctt
    22621 ttcacctcgc atcccgacaa cccggagatc ctcgcggtcc ggcttggcgc cctcaacgag
    22681 gacccgggca tccgccccgc tgcccaccaa ttcaccgcct atgccgcgcc ctggttcccg
    22741 gtgccggatg acggcctgcc caaattcccc gaaagactcg actggaccca ggttctcggg
    22801 caacagtaag cagagcccct cccagcgatt acttccttcg ggtgggaggg gccggtttcc
    22861 atgggaggtc cggcgcagtc cggaccgact gcagtcaggt tcggtcggcg agcagtcgat
    22921 cgagcttcgc gtagccctcg tccatgccct tctccatgcc cgactgaagc atgccgtcgc
    22981 ggtactccct agcctcgaat gtagtgaggg tcaccagtct ggtccggccg ccttcgcagg
    23041 ggatcagttc gagccggtcg atcgaaggcg gcggctgggg ctccatcacg aaattgaagg
    23101 tctgggaaag cttccgcggc tcctcgatct cgaggaactc gccgaagaag acgtattccg
    23161 cgccggactc cgggtccttg tggacgtacc ggtatttgcc gcccgggcgc acgtcgtact
    23221 cctccaccgt catctccagc cgatcgggcc ccacccactg ggcgagcagt tcgggatcgg
    23281 tccagaagtc gaagatgact tccggtggcg cgtcgaagat ccgctcagtc cggatctcgg
    23341 tgtcgctcgg ggtggtgacc agggtcttgt gcatttcggt caattcactc atttctcctg
    23401 ctccttcttt tgctctttct tctcttcgat cgcttcttcc agccggttca gctgctggtg
    23461 acggcgacga cggaactcgt cgagccagaa cgcggcctcg tcaaagcctt cggtcgccag
    23521 ctcgcaccag cgggtccttc cccgcttctc ggtccggacc agccctgcct cctcgaggat
    23581 ccggatgtgt ttcttcatcc cggtcagggt catcccggct ggcccggcaa gctcggaaat
    23641 ggaggccccg ccctcgccca gccggtcgag aaccgccagc cgaacgggat cggccagggc
    23701 cgcaaaagcc cgctccgccg aacccgctcg ctcatctaga tactgaacca tacggttcac
    23761 tataacacac cccattccca aagcccgatt ttcgccaaat accgcaccca actccgacct
    23821 cgtctcaacc cccaaagacc gagatcgcgt ccgtcggcgc tatccgccga caaaccgcat
    23881 ctcgcgaaat gccaccgcca caaaccccga gcgggtcgtg cacagggacc tccagccgtt
    23941 cgctggcaag gagacaggcc ggaaatgacc gcgatagtac gcgggtacat gagctgtcgt
    24001 ttccggccga cgacgaagcc agcggacggc tggtggttcc tgggcgcgtt gcccgggccc
    24061 cgggggcgtt agcgggtgta agtgatctgc cgccgcttct cctggcggcg cgcattcttc
    24121 gagtaggggt cgagttcgac cggggcgctg gcgatcgcct gggtccggag gtcggaccag
    24181 gtgaggcctt cgcggaccag ggccggcact ccgagcgcgg cggcggtcgc catttcaact
    24241 gcctggagga tcacgccgta ggcgagcgcg tcggcggtcg agacgcccca gcccttgtgg
    24301 aggacgaaga tcacggcgag ctggaagacg ccgatgttcg acggggtggc cggcacgatg
    24361 gcggtcacgt tgacggcgag gagcacggcc gcggcggcgg cgagcccggc ctggctttcg
    24421 aggccgaggg cgaacatcag ggcccagcag gagagcatct ggatgaccca ggcaaagagc
    24481 tggagggcaa cggccggacc gccgttgcgg gggtgccgga acacggtcag gccgcggcgg
    24541 acctggagca tcaccgagtg gatggcggac ccgatccggg ccagccggcc cttgccgttc
    24601 tgggcgccga gcagggtcgg tgcggcgatc accgccagga gcagggcggc cggaatcatg
    24661 ctgtagagca ggatctgttt ggtcgcgccg tggaagacgt cgtccatcga ggtcagcacg
    24721 aaggcgccga gcaagaggat cgcgaggatg ttcagcatcg tctgcgagac gatcgtcccg
    24781 atcaccacgg gcagggtgtc cttggcccgg ccggtgtggc gggccaggct gatcgccctc
    24841 gcgggttcgc ccagacgggc gggcaaggtc gcggacatca gcaccccgat catcgtggcg
    24901 gaagcgaagt cacggcgctt gaggccggaa tgcggcatcg ccgaacgcgc gatgaagaac
    24961 cacgaccagg cgcggaagaa gagcgaggat gccatcaggg caagcgcgat gagaacccag
    25021 gagatgtcgg aatcgaccag cttgtcggcg acctgatcga caccgatctt ctgtgcggcg
    25081 atcgcggtga ggccaagtcc gaacagtgcc atcgccgcaa gaccggcctt gcgcagccag
    25141 gacttgccgc cgccggaagg cccggatgcg gggacgggat cgagcgaggg cagtttgacg
    25201 gccggagccc agggcagccc gtcggaaggt gcgatgccga tccggcgccg gacccggtcg
    25261 accgtgtcct ctggttcggg aacctcgatc gcccgttcgt acacatccat cacctggtcg
    25321 gcgacgttgg accaggcgta gcgctgggcg ctttcgcggg cggcctcgcc catctcggcc
    25381 agacgctccg gctcgtggtg ggcccactgg agttcctcgg ccagggcctg cggatcggcg
    25441 ggcgggacga ggatgccgtc atggttgtga gtgaccacgt cgctgtagcc ggcgatgccg
    25501 gaagcgatca ccggcgtgcc gacggcaaag gcctcggtca ggatcatgcc gaaggactcg
    25561 ccggaaagcg agggagcgac cagcacatcg gcgtcgtgca gctgctgcca gagctcttcg
    25621 ttggaaaccc ggccgtgcag atcgaggtgg tcggccagtt cggggtgggc gaggatgcgc
    25681 tggacgtcct cgggaccggc tccaaccact gaaagccggg ccggaacatg gtcgaccagg
    25741 gccgaaaagg cgcgcagcag gaccggcagg cccttgcgtt catccggccg gccaacgaag
    25801 agggcccgca ggtggtcggt ctccggtttg ggcccgtccg gggccgcgtc gacgtccaca
    25861 ccgttgggga cgatcgtgta gttgccgccg taccaacgcc gaccggtcca ggcggcggcc
    25921 tgcgaaacgg cgattcgggc cgagagccgg ttcagggtgc ggcgggcgcc gaccgcgttg
    25981 gccagatagt tcggcaccgg cttggtcgag taggcgtgga aggtgccgac caccggaacg
    26041 tcgctggaca agcatgtgtc ccagccgttg gccggcgtgg tcggctcgtg cagatggacc
    26101 acgtcaaagt tgccttcttc gatcgcccgg cgggtctgcg tcacgcccga ggggaagacc
    26161 ggaatgttcg aaacggcccc gttcgcgttg acgccgaagg tgcggccgac cgggatcagg
    26221 taatcgggaa tctcgcggtc ttcggccggg gcacgatgaa gaaccttcga gaggcggtcg
    26281 ggtggatcga agggcgcgat gacgcggacg tagtggcctc gggagatgta ctcctccgcc
    26341 agcgactgga catgccggtt aacgccgccc tgataggtcc acgaatacgg tgtgacgagg
    26401 gcgattcgca cgatagaaca ggatagtgaa ggtggcggct ttccgaggga cggttaaccc
    26461 gattctgggt ggtaattcga cgtcgtccac gcaaatcccg ctaccagcgc cagataaagc
    26521 gttccgacct ggatgaagag ggcgccggaa tcgttggtca gcgcaccgat cagggagccc
    26581 gcggcggcgg ccgcaaagcc ggctttcatc atcggcgaat cctcaaacca ggcgagcacg
    26641 atctcccgct tccagatgcc gatcgcgatc accgccagtg cggccagggt caccgggatc
    26701 cgcgagacct tggtgaagct ttccagggtg gcgtcgagcc ggtggccgac cacctcgaaa
    26761 aacgaccctc cggacccgcc cagcaccgac ctcacgaagt gggtctcact gccgctcacg
    26821 atgtccgcca gtgcgaccag caggagcgcg gcgaaagccg cggcgaggcc cgcccatgcc
    26881 agtttcgggc ggccgacggc gaaggcggcg ccaacccccg ccgccaccgg gaagacgatc
    26941 gccgcaccga cgtcggcgcc gaaccggccg gccgcgaaga cgaccgtggc caccaccccg
    27001 gccacgagaa agctgatcgc ggcgcgacgg cccccggccc gggccctggt cagggccgct
    27061 ccggtgccga tcgaggtgag gatcatcagg gtcgattcga gttcgttgcc gatcccgtag
    27121 aagcgtgcac caagtccggg attggggccg atcaccgccc tgggggtcag tgccgatccg
    27181 gccagcatgt cgatcccgta ggggatcacg gtgaggccgc aggccagggc gagggactgc
    27241 cagccgggag cgattcgcat aagcagggcc gcgatcagga ccggcagcag ggtcgcgatc
    27301 gccctttcga tcgccagcgt cggctcgagc gccgcggtca ggagcagcac gaccggcagc
    27361 agaacggtgg cgaggcagag cagctggagc gccggcctcg agtaaaggcc gcgacttccg
    27421 aacgagacca gggccgccag cccgatccag agcagcagcg gaatcgcgag cgccgcgccg
    27481 cgccgtttgc cgacctcctc gtagcggctt tcgagatcgg ccagccggtc gatgtccagc
    27541 tcgccccccg actcgatcgg caatccggtc atcgccttgg gggccttgat gtcgaagtgc
    27601 cgcaggatcg tcggggcgat gtcggtcgag agcacatatc cgtcggtcct cgtactcggt
    27661 gacctgagtt caccgtccat gcccgggccg gcgatggcga tcgaaagctg atcgccggag
    27721 tcggccgggg ggcgctcgat gaatatctgc atgtcccgcg gatttcggtt tgcggcggct
    27781 tcgaaggcag aatcgagcgg catcgaaagg acggtgaccg ggtccgggca gccgcggatc
    27841 gccgacttcg cccccgggga atcggcgttg agcatcgaag ctgggccacc gcaggcctgc
    27901 ggggcgcggg cgatcccccc gtcacggcgg gccaccgcca gcgcggctgc tccggccgag
    27961 tcggcagcca cggcgtcgag gtcgttctgg ctgaggacgg tggccatcaa ccccgggatt
    28021 accttgctgt ccgtgcttct ggcccgtttc agaacttttt cccatccgtg cgcgaattct
    28081 ccacctggat acacgcggtc taacggtgtg tcatacgagg agtcaaacgc cctcgctccc
    28141 tgggagacgt caatccatgt ctgctgggcg gggacgtcgc ccagacccgg attcatcagg
    28201 ccgatcgccg cgttgtcgag cccggacagg tcggcgggcg aggttccggg gggcagaacg
    28261 atgatcgtca cctggccgcg atggccggtc gcggcgtcgg cgacctggag atcatcggcc
    28321 ccggccgcgc cgacccggac gagcgaaaac acaaggccga gcagcagacc gaaggtgacc
    28381 gcgcgggtgc gcgaaaggac ccgtcggact tcggaattcg ccggtccggt ggccctcccc
    28441 ggtatcttca gaaacggcat ggcaacggaa acgataggtc ctgaagcccc cgttctaccc
    28501 gacgtccagc acgcgcaggt cggtgaggtc gaaatcgcct ttgatgttca cggcgatccg
    28561 aacgaccccg tgttgcttgt aattcccggc ctcggcaccc agatggtcta ctgggacatg
    28621 cccctcgtcg aaatgctgac cgaccgcggt ttccgcgtag tccggattga caaccgggac
    28681 aacggcgctt cgacggtgct tcaccacaag ggcaaacccg ccctgccggc gatgatgttc
    28741 gggatccccc gcgacctcgc ctacacgctc aacgacatgg cgaacgatgc ggccggcgtg
    28801 ctcgaccacc tcggggtcga tgccgcccac gtgatcggtt actcgatggg cggcatgatc
    28861 tcccagacca tggcgatcga ccatccggat cgggtccttt cactgacctc gatgatgtcc
    28921 cggaccggtg cgcggctcga cgcgttccct ggcgtgaagg aactggccgc cctgatgcgg
    28981 acccgcccga ccgacagcct ggacagttac ctgaccggca tggaagcgct ggcggcaatc
    29041 atcggttcgc ctgatttccc gaccaacaag gaacggcttc ggaatatcgc cacagtcgcc
    29101 tatcaccggg ggattcacct cgagggaacg gcgcggcagc tccacgccat caactgtcag
    29161 ccgaagcggg accgggccct gagccggctc gacatcccca cggtcgtcct tcacggctcc
    29221 gccgacaagc tggtcttccc gcgcggcggc cgggccaccg cccgcgccat tcccggcgcg
    29281 gagctccgga tctacgaagg aatgggccac gacatccccg agcagctctg gccccgcttc
    29341 gtcgaggaga tcgacctggt cgcccggcga gccggcaagt agccggggtc acgatcgcgg
    29401 tggcagggat tccttatgct ggatgaccgc atgagtaacc caggaacccc cgaaaacccg
    29461 cttcgggtag cgatcatcgg cgccggacca tccggcttct acgctgccga ccagatcatc
    29521 aaagatcccg agacccacgc ccaggtggat ctctttgacc gcctgcccac cccctacggg
    29581 ctggtgcgcg gcggtgtcgc cccggaccat ccgaagatca agtcggtgac ccgggtctac
    29641 gagaagaccg ctgccaagga ggggttccgc ttcttcggca acgtcgaggt cggccgcgac
    29701 ctgaacgtcg aggatttcga gcagcactac cacgcgatca tctacacctt cggggccgaa
    29761 gcggaccgtg agctcggcat ccccggcgag gatctgcccg gcagctatgc cgccactgcc
    29821 ttcgtcggct ggtacaacgc ccaccccgat tacgccgacc ggaacttcaa gctggaccag
    29881 gcgaaacggg cggtcgtgat cggcaacggc aacgtcgccc tcgatgtcgc ccgcatgctc
    29941 gccctcgatg acgccgaact gcggaagacc gacatcgccg accacgcgat cgagttgctc
    30001 gacgcatcga cgatcgagga aatcgttgtg ctcggccggc gcggcccggc ccaggctgcc
    30061 tacaccaacc ccgaggtcaa ggagctcggc gagctcgagc aggcggatgt gatcgtaagt
    30121 ccggaggaga tcgagctcga cccggccagc aaggcgtgga tcgagtcgga cgaggccgac
    30181 aagaccgccc gggtcaacgt cgagatcgtt caggagtacg ccacgcgcca gcccaccggc
    30241 aagccgaaga aggtcgcgct ccgctttctc tcctcgccgg tcgaaatcaa gggtggcgac
    30301 agggtcgaaa gcatcgtgat cggccgcaac gagttggtcg acgtcgacgg caatctcaaa
    30361 gcccgcgaca ccggcgagcg cgaggaactg gcttgtgacc tgatcctgcg ctcggtcgga
    30421 tacaccgggg tcccgcttcg cggggttccc ttcgacgaac ggcggggcac gatcctcaac
    30481 gaggacggac gggtgctcga aagccacggc ggggatcacc gcaccggcca ctacacggcc
    30541 ggctggatca agcgtggccc ctccggcgtg atcggcacca acaagaaatg cgccaccgaa
    30601 accgtggccc atgtcttcga ggacgttgcc gccggcagac tcctcgatcc cgcgagcccg
    30661 gatccgaatt cgatcgagga gctcctcagg gagcgcggaa cccactacgt gagtttcagc
    30721 ggctgggaga agatcgacgc ggccgaggtc ggcaagggtg aaccgcatgg ccggccccgg
    30781 gtcaagttcg tacggatcga agagatgctc gaaaccgcgg ccgagcaggc cgaagcgggc
    30841 gctgcaacgg ggggcgagtg atggccgccg acctggccga gctcaagggc atgatcgagg
    30901 ccgcgctgcc cggctcgagc gccgaggtga tcgacgaaac cggcaccggc gatcacctgc
    30961 gggccgaggt ttcggcaccg cagttcgagg gtcaaagccg gatcaaccag caccggatgg
    31021 tcaaggccgc ggttcgggag cgcttcgatg acggcaccat ccacgccctg accatcaaga
    31081 tcctggatta ggttcaagat tggtccccgg ttgcggcgga cctgccccca acggcaccgg
    31141 tccgaagcta ctgctgctgc ccgccaatga aggtcgaccc gaccaggggt actcccggcc
    31201 ggtagacgaa atctgcgtgc gaagcggctt cgagcaggat ggcgtcggcg cgggcaccgg
    31261 gggccagtcg cccgatgtcg gtacggccga gcgccgcggc gccccccagg gtcgccgcgc
    31321 ggatcgcctc ctcgatcgtc atccccatgt cccttacggc gagcgcaagg cagaatccca
    31381 tcgcggtggt gttgctggaa ccggggttgg tgttcgttgc gatcgcgacc tcgacccccg
    31441 cgtcgatcgc acgccgggca tcgggatagg gctgcctggt cgagaagtcg gtggcaggca
    31501 ggaaggtcgc cacggtcgag cccgaggcga gcgcgtcgag gtccgcgtct tcgaggtagg
    31561 tgcagtggtc gaccgaagcc gctcccatct cgaccgccag ctgcaccccc gggccgggac
    31621 cgagctggtt tccgtgcacg cgcaggccga ggcccgcttc ccggccggcc tccagcaccc
    31681 ggcgtgattg ttcacggtcg aaggcgccga cctcgcagaa cacgtcgatc cagcgggcgt
    31741 gcggggcgca ggcggccagc atctccccgc aaaccagttc gacatagtca tccgcccggt
    31801 cctcgaaccc ggccgggacg acatgcgccc cgaggaaagt cacatcgtcg gtaaatccgg
    31861 ccgccacctc caggcacctc agctcggagg ggatgtcgag cccgtagcct gacttgatct
    31921 ccgtgtgggt gatcccggcc cggatcgcct cccggcgacg gcggtcggtc aagccggcga
    31981 gttcatcccg gccggccgcg cgggtcgcct ccaccgtgac cctgatcccg cccgccgagt
    32041 agggctcgcc ggccatccgg gccgcgaact cgcgggagcg gtcaccggca aagacgagat
    32101 gggtgtggct gtccacgaaa cccggcatta cgcagcgtcc gccggcgtcg acgtgacggt
    32161 ccgcctgggc actcccggcc ggtccgatct ccgcgacgac gccttcctcg aaggtgattg
    32221 atgtgttctc gaccgcgccg agcatgccgc tgccaagctg cggatcgttg gtcaagagca
    32281 ggccgatgtt ttcgatcgcg aggctgctca cgacttgcct tccacggcct cgatcgaggc
    32341 cgcgagttcg gccgggacgt cgatctgccg gtgcctgcct tcggcgacga tcgtgcgccc
    32401 ggcgaccatc acccgctgca cgtccccggc gttcgcggcg aacagcagcg acccgaccgg
    32461 gtccgccggt gcggtgccgg ccatgcggac gctgtccagg ccgaccgtga ccaggtcggc
    32521 cggggccccc ggagcaatcc ggcccccctc cgcccatccc agttgccggt aacccgcggc
    32581 cgaggcgatg tccatcagcg gcccgcagcc caacaccccc cgctgaccgg tcgcgaggcg
    32641 ctggtcgagt tcaaccgccc gcgtttcctc gatcgggtcg atgaccgcgt tggagtcggt
    32701 gccgatcgag atcgaggctc ccccgtcgag cagccggcgg gccgggccga tgccgtcggc
    32761 caggtcacgc tcggtggtcg ggcagaggca gacgccgccg ccaccgaggc gagcgaagtc
    32821 accctcgtcg agatgggtcg catgaaccgc ggtgaagcgg ggcccggtcg cgccggcagc
    32881 ttccagcagc gcgctcggcg tggcgccgta ggccgcctgg caggcttcgt tttccgccgg
    32941 ctgctcggaa acgtgcgcat ggagcggagc ttcgcgagcg gaggcccatt ccgcgacccg
    33001 ggcagccgag ccggggtcga ccgcccggac gctgtgtatc gcggcgccga cgcgggccat
    33061 cggaatcccg ccgttctcct ggccgtggct ggctgccacc tcgccgtcga tcagctcgac
    33121 ccgctccgcc caggcatcgg cgtcgccatc cgagaaacgg atctgggcct cgtcgggggt
    33181 cgtgtcgatg ccgccgtgga ggtaacaggt atcgagcagg gtgatcctga tccccgcccg
    33241 ccgggccgcc tccaggatcg cccggcccat cgcgttggga tcgtcgtaac gcccgccgcc
    33301 gggtgcgtgg tggaggtaat ggaactcccc gaccacggtg atcccggcct ccgccatctc
    33361 ggcgaaggta gccgtcgcca aggccaggta gcggtccggg tcaagcccgt caagggccgc
    33421 gtacatctgc cgccgccagg tccagaagtc accgtggccg gaatgggtgc ggccgcgcag
    33481 gatccgctgg aaagcatgcg agtggacgtt ggcgaaaccg ggcacggtga ggcccggcag
    33541 ccgttcggct tccccgggcg ggtccgttcc cggctcgacc gaggcgattc tgccggcctc
    33601 gatccctatc gtgaccgacg cggcggcctg ctcgccaccc agccaggcgt attcgcacca
    33661 cagttcgatc gccggttccg ggctcaagag ccgacccgtt cgacgaaccg ggtgatccgc
    33721 tcgacgaact cgtcccgctg gtggcgttcg aactcgtccc aggtggcaag actggccgta
    33781 tcggtgagcc ggtcgaggaa gagcctgccg tcgagatggt cgacctcatg ctggacggtg
    33841 ccggcggtca ggcccctttt gacctcgtcg tgctcgaccc cgtcccggtc caggtacctc
    33901 accctcacct cgacgtgacg caggacgttg ccgcggaggt ccggcaccga gaggcaaccc
    33961 tcgttgatct cgaccatctc ctccccgatc ggctcgacca ccggattcac catgaccagc
    34021 cgcgggatcg gtggcttgta cgggtacctc gggtttactc cctccacttc ggccaccgcg
    34081 acgcgcaggt tcgagccgat ctggttggcc gcgaggccgg cgccattcgc ggcccggcgg
    34141 gtgtcgatca ggtcgtcgat cagctgctgg acctcgggtc cggccagctc ctccgggtcc
    34201 accgggcggg tcggctcacg gaggaccgga ttgccgatca cggttatctc ccggacgctc
    34261 atccccgctc gccgtcacgc atcgggatcg ctaccccgcg ctcgccggcc acttcgatgg
    34321 cgcggtcata accggcgtct gcatggcgaa tcacgcccat gccgggatcg gcggtgagga
    34381 cccgttccag cttctgtgcc gccaggtccg tgccatcggc aacgcagacc atgccggcgt
    34441 ggatcgagcg gccgatgccg actccgcccc cgtggtggat gctcacccag gtcgcccccg
    34501 cggcagtgtt caccatcgcg ttcaggagcg gccagtcggc gatcgcatcg gagccgtcgg
    34561 ccatcgattc ggtttcacgg tagggggaag cgaccgaacc ggagtcgagg tgatcacggc
    34621 cgatcacgat cggacccttg acctcgccgg tccggaccat ctcgttgaac ctcaggccga
    34681 gccggtgacg ctctccgtat ccggcccagc agatccgtga cggcaggccc tggaaggcga
    34741 tccgctcgcc ggccagccgg atccagcggg ccagcgactc gtcgccgggc agctcctcca
    34801 ggaccgcccg gtcggttgcg gcgatgtcgg ccgggtcgcc ggagagcgcg gcccagcgga
    34861 aggggccctt gccttcgcag aacatcgggc ggatgtaggc cgggacgaac ccgggatagt
    34921 cgaaggcccg ctcgaacccg ccctcacggg cttcggcccg caggctgttt ccgtagtcga
    34981 acacctcggc cccgtcgtcg aggaagccga ccatggccgc gcaatgggcg gccatcgcct
    35041 tgcgcgagcg gtggacgtac tcgaccgggt cggagaggcg cagcgcgtcg gcctcatccg
    35101 gggtcatcag gttcgggaca tagccgacca gggggtcatg ggcgctggtc tggtcggtca
    35161 cgatgtccgc cgggaatccg atccggcgca gttccgggag gacatccgcc gcgttggcgc
    35221 agagtccgac gctgagcgcc cggccggccg ccttcgccga ctcgcagcgg gagaccgcgt
    35281 cctcgaggct gtcggcctgc tcgtccaggt atctggtctc gaggcgccgc ctgatgcgct
    35341 ccggttcgat ctcgatgcag agggcgaccc cgtcgttcat ggtcacggcg agcggctggg
    35401 cgccgcccat cccgccgagg cccgcggtca ccgtgatcgt tcccttgagc gagccgccga
    35461 agcggcggcg ggcgatctcg gcgaaacatt cgtaggtgcc ctgcacgatc ccctggctgc
    35521 cgatgtagat ccaggagccg gcggtcatct gcccgtacat ggtcagcccc agttgctcga
    35581 gccggcggaa ctcgtcccag ttggcccagt cgggcaccag attggagttg gcgatcagca
    35641 cccgtggcga ccattcgtgg gtgcggaaca ccccgaccgg cttgcccgac tggaccagga
    35701 gagtctggtc gtcgtccagc ttgcgcaggc tggcgacgat cgcgtcgaag gcctgccagg
    35761 aacgggcggc ccgtcccgtc ccgccgtaga cgaccaggtc gtcgggactt tcggcgacct
    35821 ccgggtcgag gttgttcatc agcatccgca ggggcgcctc ctgctgccag cccttgcagc
    35881 tcagctcgtt tcctcgcgga gcgcgaacag cgcgggcacc gggcagacca tcgctcatga
    35941 caactctccg atctcttctt ccaccgcggc gagtacctcg ccttcgcgga ctattcgttc
    36001 cactgccgcc agttccggcg actgccaccg gtccgggccg ggcccgggcg cgcattcccg
    36061 caccgcccgc agcgccgcag cagtcccggg agcgggtgcg agcggcgccc tgagatccag
    36121 ccccctggtg gcgcaaagca gttcgaccgt gacgatcctt tccaggttcg cgaccgagcg
    36181 gcgcagcttc cgggccgcgt tccaggccat cgagacatgg tcctcctgca tcgcactggt
    36241 cgggaccgtg tcgctggaag ccggaaccgc aagctgccgg ttgtcggcga ccatcgccgc
    36301 ctgcgtgtac tgggcgatca tcatccccga gtcgaccccg ggatcgtcgg ccaggaaagg
    36361 cggcagaccg tgggaccggt tggcgtcgag cagccggtcg gtgcgccgct cggagattgc
    36421 cccggcctgg gctgaggcga tgcagagcag gtcgcagaca attccgagcg gtgcgccgtg
    36481 aaagttgccg cacgattcga cccggccgtc cgggagaacc atcggattgt cgatcgccga
    36541 atccatttca acctcggcga cccggcgggc atgcgtgacc gagtcacggg cggcaccgag
    36601 gacctggggg gcgcagcgca gcgagtaggc gtcctggacc cgggggtcgc cttgccggtg
    36661 gctcgcgacg atctccgagc ccgccaggag cttcatcatg ttttcggcgc tttccatctg
    36721 ccccggttgc ggccggagac cgaccaggtc ggcagcgaag gcacgatcgg taccgagcag
    36781 ggcctcgacc gaggccgcgg cggcgatgtc ggcggtgcgg agggtccgtt cgaggtcaca
    36841 gatcgcgagt acgagcatgc cgaggatccc gtcggtgccg ttgatcagcg ccagcccctc
    36901 cttcgaacgc agcttgagcg gttcgatccc ggccgatcgc agcgcctccc cggacgggat
    36961 ccgggagccg ccggggccca gcacctcgcc ttcgccgatc aggaccaggg cgcaatgtgc
    37021 gagcggcgcg agatcaccgc tcgccccgag cgatccgtac tcgggcacca ccggggacag
    37081 cccggagttg agcagcgcca gcagggtctc ggcgaccacc ggccgggcac cggacctcgc
    37141 catcgccagg ctgcgcgccc tcatcagcat caggccccgg accacctcgg cctcgaccgg
    37201 ttcccccatc cccgccgcgt gggaacgaat cagggaaagc tggagctgct cgcggcggtc
    37261 ggccgggatc gaggtcgtcg caagcgaacc gaaaccggtc gagaccccgt aaacgggctc
    37321 gtcgctgttg ccgagccgct cgacgatccg ggccgattcc tccattcgtg cgaccgctgc
    37381 gccggtcagg gccaccctgg ccccgaagcg cgcgacgtcc atcacctccc cgaaccgcag
    37441 ccccttgcca tcgagccgga tctcatcggc ggtctgcttc gcgctcattt ggcgttcgat
    37501 cccacgacgg cgatcgagtc taacgccgcg cggggcaagc ttccgggatt tccgcccctc
    37561 ccgccgaatt cgctgaccgc ccgggccctt ccccgttgac cgcgccgcgc cgcacgggtc
    37621 cggagagtgg ccagggtcta acatcagtta catggctgac caggaactca cagatcgcgt
    37681 ccgcgaactg atcaacgaga actccgtgct gctcttcatg aagggcaccc ccgaggcccc
    37741 gcgctgcggg ttttcgatgc gcaccgttca ggtgctcgac cagatgggag tcgagtacgg
    37801 agcaattgac gtactgcccg cccttcagcc cctgcgcgaa gtaaccagcg aaatctccga
    37861 ctgggagacc ttcccccagc tctatgtgaa cggcgagctg gttggcggct gcgacatcgt
    37921 cgaggaaatg tttgacaacg gcgaactggc cgaggctctc ggggtcgagc agcccgaacc
    37981 cgccgaaccg gatttcgaaa cctccgctcc cggcgagccg tacaagagcc cgccgatgaa
    38041 tattgagggc gcgtagcgct taaccggcgt gggtgccgta ggcgagctta agggaccgtc
    38101 aactgacgga gtccgatcat caattcattg atgccgtgat cggacgggtt tctgtggcat
    38161 tccggtgcag accggaatgc atgcggttaa acagtggcgg cttcggcgtc gtccttcacc
    38221 tggaaggaag acccgcagcc acaggcagct acgacgttcg ggttgttgac ctggaacccg
    38281 gccccggtca gaccctcgac gtagtcaact tccgagccga agacgaactg catgctgacc
    38341 ttgtcgacga tcacggaaac cccgcgatgc tcgaagacat gatcgtcttc acccctggtg
    38401 tcgaaggcaa gcgcgtactg aaaccccgag cagcctccac cccgcactgc aacgcgcagc
    38461 gactgctcct cgccctcggg agaatcaccg atcagttcgg cgatcttctc ggcggcggct
    38521 tcagtcaggg tgattgcgga ttcgggctgc tcggacatgg ttaaagagta gcgcgtgtgc
    38581 ccggcgtggt ctgtgacggg ggaacagttt tcccgcctgc tttgcccgac ccggcccggt
    38641 gtctgataga accgttgggt gtccgccgag tccaccatcg cgctggtgac cgattcgacc
    38701 gcgtcgctca cccgggagga tcgtgagcgc ctcgaaatca gcgtggtcaa cctctatgtg
    38761 atcctcaacg gcgagcaacg gcccgagaac gagatcgacg actacgccgc cttctacaag
    38821 gcggtgctcg aatcgccgca gctcaccacc acttcgcaac cctcgatcgg ggatttcatg
    38881 gaggtctacg ggccgcttct cgacgagggc agggagatcg tctcgctgca cctctcgggc
    38941 gcgatctccg gaacctgcga ggccgcccgg caggccgcgg tccagctcga agccgacggt
    39001 cgcggcgggg aacggatcac cgtagtcgac tcccgcacca ccgccggcgg cctggccttc
    39061 tgcaatctcg ccgccgcgaa cgcgatcaag gacggcaagg acgtggccgg ggtcgtcgcc
    39121 cagatcgagg cctgccgcga ggagatcgac acgctgatcc tggttgacac cctcgagtac
    39181 ctgagaaagg gcggccggat cggcggggcc caggcctgga tcggcggggc gctcaagatc
    39241 aagccgctga tcaccctccg cgagaccgtc gagccggtcg acaaggtgcg aaccctctcc
    39301 cgtgccatgc agcggctgaa ggatcacgcc agcgagaaag ccgcctccgg gcaggaaatc
    39361 acctggaccg cccaacacat ccaggctccg gaactggcgg ccgaactggc cgcccactgc
    39421 cgcgaggtct ttcagtgcga aggagcattc ctcgacgaga tgtcgaccgt gctgggatgt
    39481 cacgccggcc cggggctggt cgcgatcgg
//
LOCUS       DAOQKN010000004        32242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_533_length_32242_cov_6.092926, whole genome shotgun sequence.
ACCESSION   DAOQKN010000004 DAOQKN010000000
VERSION     DAOQKN010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 32242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32242
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_533_length_32242_cov_6.092926"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="PLJ64_00565"
     CDS             <1..301
                     /locus_tag="PLJ64_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HNC92016.1"
                     /translation="VRDVATRGWLPVPVFCAGGIATPADAALMMQLGAEGNFVGSGIF
                     KSDDPERRAKAIVEATTHFNDADRIAAASEQLGEAMPGIEMAALGEDGRLAGRGW"
     gene            363..899
                     /gene="pdxT"
                     /locus_tag="PLJ64_00570"
     CDS             363..899
                     /gene="pdxT"
                     /locus_tag="PLJ64_00570"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HNC92017.1"
                     /translation="MLAVQGGFQRHVEHLREAGAEPVEVRLPAELEALDGLILPGGES
                     TTITMGIEKAGLAKGILAHHQAGRPTLGTCAGMIVAGREHLGLIDITTKRNAFGRQLS
                     SFEDDISVEGVVDGPIRAVFIRAPWVESTGEGVQVLAEVRGHPVAVRQGNVTALSFHP
                     ELTPDNRIHQWFKELCVA"
     gene            complement(985..1416)
                     /locus_tag="PLJ64_00575"
     CDS             complement(985..1416)
                     /locus_tag="PLJ64_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92018.1"
                     /translation="MIPEGKHRASRTVAAGAVAGVMLLAALSLWSVIPLVWLWVGSQF
                     ADSQFPSLGPYLIVLFGAIASILLVAWILGRLNEIYIRLTGARDVGPIRMGWMKSLRD
                     SEKSGDPPSLIEVVIIGSVVIAFIAFIAWFFLAAGSPLPNS"
     gene            1445..2197
                     /locus_tag="PLJ64_00580"
     CDS             1445..2197
                     /locus_tag="PLJ64_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNC92019.1"
                     /translation="MSGHSKWSSIKHKKGAADAKRGQLFTKLARAITIAAREGGGDPD
                     GNPALATAIQKARAQSMPKANIEKAIDRGTGEGDDAVVVERIQFEGYGPGGAAILVDA
                     LTDNRNRTSAEVRHAFTKHNGSLGEPGSVAWMFETFGVVVVDGEANSEDDLMGAIDAG
                     ASDVVEEEGKLRVLAEPTNLTAVRQAIEADGIEIESAGLEVIPKNTVDIEESDAGTLI
                     KLLEAIEDQDDVNEVHANFDIPDEVLEKIAAD"
     gene            2249..2740
                     /locus_tag="PLJ64_00585"
     CDS             2249..2740
                     /locus_tag="PLJ64_00585"
                     /inference="COORDINATES: protein motif:HMM:NF025734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HNC92020.1"
                     /translation="MESEGGRWSRASWAKRIGWIFGGLVALFLVIQLVPYGRDHTNPP
                     VTRSPKFVGAQTEQIFNDSCGDCHSNLTKWPWYSNVAPVSWLVQSDVDEGRSIMNFSE
                     WDKPQPAIDELVEQINEGSMPPSKYTRIHSGTKLSDSEKQTLINGLIATYRANPPGGI
                     KGG"
     gene            2777..3310
                     /gene="ruvC"
                     /locus_tag="PLJ64_00590"
     CDS             2777..3310
                     /gene="ruvC"
                     /locus_tag="PLJ64_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNC92021.1"
                     /translation="MKVVMGIDPGTANLGFGVVRIEGGHMVALDGGVIETASGDPLER
                     RLGEIHSSLSRLIKWHQPVALAIEDLYFGKNVGSAMKVGQASGVSMLAAAQCDVPCFS
                     YTPQAIKTAVCGSGNAAKRQVQKMVATLLNLSEVPQPDHAADALAVAICHGGTAGRRD
                     ATEAALAGSGSSLRVAS"
     gene            3307..3906
                     /gene="ruvA"
                     /locus_tag="PLJ64_00595"
     CDS             3307..3906
                     /gene="ruvA"
                     /locus_tag="PLJ64_00595"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNC92022.1"
                     /translation="MIATVRGEVLVRRSDYVVIEAAGVGYRLAVSSETLRSVPAAGQE
                     IFLHAETISRDDSLLLYGFSSEEERDLFNMLIGVSGIGPKVAISALSGGPHRELTRAI
                     IAGDAKRFQAVPGIGKRTAERIILELKDKVTSVAEEAGEAVLPAASDARSLARDGLIN
                     LGYAPLEAEQLLDGVGDGDDPQQLIGAALKRAAASGRGE"
     gene            3908..4993
                     /gene="ruvB"
                     /locus_tag="PLJ64_00600"
     CDS             3908..4993
                     /gene="ruvB"
                     /locus_tag="PLJ64_00600"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNC92023.1"
                     /translation="MSDLPDFGGKRIDLGVEADLPEGAELRVHDARADQPDLELDQSL
                     RPKGLGDFVNQKQVTDQLAVFIEAARRRGEPLDHVLLAGPPGLGKTSLANIVAAEMGV
                     PLIQTAGPALERKGDVAAFLTSLEPGSVFFIDEIHRLGRAVEETLYPAMEDGELPVVL
                     GQGAGAQTVTLPLPPFTLIGATTRSGLLTTPLRDRFGVSHRLEYYTPADLARIVIRSA
                     GILGVEIDEGGAVAIAERSRGTPRVANRLLKRVRDFAEVKGSGSVDAEMADAALEMLE
                     VDKAGLDRADRALLELIATRFQGGPVGLSTIAVAIGEEQDTIEDVLEPYLLQMGMLKR
                     TPRGRVLTQSAFEHLGLPVPSGHQSLF"
     gene            complement(4990..5901)
                     /locus_tag="PLJ64_00605"
     CDS             complement(4990..5901)
                     /locus_tag="PLJ64_00605"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC92024.1"
                     /translation="MKIFIAGASGAIGLPLVRQLAAAGHEVVGSTRGGQGAARIREAG
                     GIAVACDVFDRAQLIEAVVKAEPEVVVNQLTSLPGKFEPKKKGFYDANNRIRSEGGDN
                     LIEATAAAGAPRVVTQSISFLYELSGPMIKAEEEPVDRSGVHDAVLGHERKSLEDDRF
                     EAVVLRYGLLYGPGTWYARDGYLSGEVRKRRLPIVGGGHGMTSFLHVEDAASAAVAAL
                     EKGEGIYNVTDDEPAATRDWIPVFAESVGAKPPRKVPFWLAALIAGKPIALQATEGRG
                     ASNAKFKAQTGWEPSIPSWRQGFFEAT"
     gene            5941..6429
                     /locus_tag="PLJ64_00610"
     CDS             5941..6429
                     /locus_tag="PLJ64_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNC92025.1"
                     /translation="MPFFICPSCGNRENSGDRTAGFSPRPKGCSKCGFGFVFELLDDY
                     YPAPNAAFFVCDQEGRVIDAGRGSFQLTGLTDEDVIGRPVREVLGLEWIDPGDGGPEA
                     DTNGDGETDPIETSLEWGVRSLGKRVEVRSEKDSAAQAIADVFPAYDDDGGLMIVLTP
                     EG"
     gene            6442..9288
                     /gene="secD"
                     /locus_tag="PLJ64_00615"
     CDS             6442..9288
                     /gene="secD"
                     /locus_tag="PLJ64_00615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNC92026.1"
                     /translation="MGGRRRNIFVLLFVLGLVIVSGIVCLKKPTQLGLDLQGGIELTL
                     QGRPTPQVKEVTSEAMDRSVDIIRSGCDKLGVSEIEVSRLGADQISVGIPGATNVGTA
                     TECATKPARLYFYDWENNLVGPARDLSLVGTGADTAEVSKQVRKAWVDAGRPPTEGFN
                     RTWIGLGAEPTMWDAVNLAAKQPRDDKCEDCTSGDKYYLFDEKTHELISGPVTAKEDL
                     YYNDQGRPIPHKGIVKKVPQGTIIVDELPTDSDGKTIDDESQAGYFVLKDNAALSGSE
                     IKDPQQNLDQFNQPNVTFDFTGDGKDSFQEITRRIAQEGQARAIGGVTSADQASALSG
                     HFAIILDGQVVSRPIINFVENPDGIDGRQGAQISGGFTVDQAQNLAELLQRGALPIDL
                     KLTSQSQVSATLGQEALDQGVLALLVGLFCVIIFLIVFYRFLGGVASLALLVYAVLFL
                     ALIKVIPITMTLPGIAGLVLTIGVAADSNIVIFERVKEELRAGRSVQSAITLGYRRGI
                     STIIDANVVTLLTAFILFVLATSGIKGFAFTLGVGTIVSLLTAVVFTQALLGSMSNTK
                     LMRSDKAFAGMEGKERWHFDFMGSAPKFFAISGVILLIGAGALTTKGLNFGIDFESGT
                     RIEVALNDSASTEDVRSVLSPLGLGSADIQTITDNPTLGDNAFQIESETLDPSEVSKV
                     DDALNKEFGVSADGFNSTSVGPTFGASVARSAALAIFFSMILIMGYVAIRFGGKYTVP
                     VLIAIAHDFLIALGIYALLGFEVSSATVAAFLTILGYSQYDTIIVFDRIRENEPKMPR
                     ATYSQIVNKSMSEVMTRSLVTSFSTVIGVIALLIFGGEVLRSFAIAILVGVASGTYSS
                     IFIASPVLALWKEHEPAFKRRRRQQIETQGSVPAFASDLEVARKDSGNGAESKAGEEG
                     AVEEAAPSQGGPNLSPRDRRRLERQKAKESGGEA"
     gene            9288..9584
                     /locus_tag="PLJ64_00620"
     CDS             9288..9584
                     /locus_tag="PLJ64_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92027.1"
                     /translation="MSIVIWFTVALALWHFTIFVPDRFWQGIIGALIGCVLGGLISGA
                     IVELILGNGLADTDIITFLAAVPGTLIGAWVIYRIGVSQGTEPIEAGSEPAEKV"
     gene            9664..12105
                     /gene="recJ"
                     /locus_tag="PLJ64_00625"
     CDS             9664..12105
                     /gene="recJ"
                     /locus_tag="PLJ64_00625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNC92028.1"
                     /translation="MATVLTRRGFGDPDAAARFLGAGESHDPFGFEGMAEVVARVRAA
                     IEAGEKITVYGDFDCDGVCATSILVGALRELGGDCDWFIPDRIDGYGLNPDSIRRLHQ
                     RGTSLVITVDCGVTSVGEVALARELGLGIIVTDHHQAETALPECPILHPEVSGYPFPS
                     LCGAAVAAKLASALRAGTSSGPERDEADLDLVALATVADVMPLVGENRHLVREGVRVA
                     RRARRVGLAALMADCRIEPSRLSSEDFGFRLGPRINAAGRLYRADAGVELFLADSRER
                     AGEIARELASVNAERRTVEREVEGLARAELKALGDPGPAVVVAGEGWHQGVVGIVASK
                     LVKATGRPSVVISIDGETGRGSARSVPGLDLHAAIGETADLLETFGGHAAAAGLSIRT
                     DRIDDFRSALGRAVASRIGTEPAEKAIEFDAVAGGADLGLDLAEEIEKLQPFGNGNPP
                     VRLFLPGARIEDLQEMGEGKHCRFAVCSGSHRATGVAFGRTSFGLAEGERADVIAELG
                     INHWNGSIEPQLRVTEVSRLPAAEPLAECEPDEWWQRFETALTRPLEGAGPAEKAEVR
                     IASGPLGLPGVALGELISSGERIVVLTADARQRWVGLGGAALGRFSSRDGEEAVGVWA
                     GSPASVSEPARDASVLLTDFTTLAGANPPVIDDFDRIALLDPPYLAASVAELARLDLP
                     VHLLAGPVEFEFTARVAAYHLDPTVQLRNLFRRFREADAIDGQATEGEPLRAVLSEDG
                     NSTRSPEQAATLLRILVETGLARTEGEGSARRAGVVSSVKADLSVSVVFSHHIELHQE
                     HRGFLSQFDRQTQTQ"
     gene            12138..14441
                     /locus_tag="PLJ64_00630"
     CDS             12138..14441
                     /locus_tag="PLJ64_00630"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HNC92029.1"
                     /translation="MPAAGTVPGNGAGIGSAAGPSAIETADLTARERSLLDDLMAVIQ
                     QFEADLATHGSSPAATIDRDEVTRAFVFACRHHADQKRKSGDEFITHPVGVARICVGM
                     ALDTETLCAALLHDTVEDTSASLEEIELEFSPTVSRLVDGVTKLTEITFESRDERQAE
                     NYRKMMVAMATDVRVILIKLADRLHNMRTLSSLPKQKQILKSRETLEIYAPLAHRLGI
                     HAIKWELEDLAFATLHPRKYSEIKHLVAQQRTERENFVEEAGEYLSDELEKVGIEAEI
                     AGRAKHFYSIYTKMTKKGREFNEIFDLTAMRVIVGSVKDCYGAIGVIHSLWKPLPGRF
                     KDYVAMPKANMYQALHTTVIGLEGKPLEIQIRTAEMHRLAEYGIAAHVAYKEGGSGTD
                     SSREKMTWLRQLVESESEQDPTEFLQSLKEDLFEDEVFVFTPKGEVMNLSAGSTPVDF
                     AYSIHTDVGHRCVGAKVNGKIVPLHYQLKSGDIVEVLTANKDRGPSRDWLKLVGTSRA
                     RNKIRAWFREESREDAERDGREALAEALRKRDLPPHKFSGSPMLADVMREMGFRKAED
                     FYIALGQGKVSARTTANKLIQRLRQGEIIEPAPVENIESGTPKRSRALREASEFGIKV
                     EGLENVALRLAKCCRPVPGDEIVGYVSLGRGITIHQANCQNVADLEKSPERMIDVAWE
                     GENEATFRVEIQIDAYDRTRLLEDLSRTFSEAGVNIISANLTTNPPMVKNRFVVEVGD
                     TGQLKQCINRIRHVESVFDAHRVNTTD"
     gene            complement(14742..14863)
                     /locus_tag="PLJ64_00635"
                     /pseudo
     CDS             complement(14742..14863)
                     /locus_tag="PLJ64_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611484.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            14968..16157
                     /locus_tag="PLJ64_00640"
     CDS             join(14968..15246,15249..16157)
                     /locus_tag="PLJ64_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611781.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HNC92030.1"
                     /translation="MPKPFPREFRDDVVAVARTGHAPIKQVAKDFGISEACLHNWLKK
                     AEIEEGTRPGLTEAERKELRETKKRIRLLEQENEVLRRAAAYLSQANLPKMIFPLVRE
                     MALAGAPIRVPVKVTCRVLGFSSQGYYKWLKEPVSDRDRDDAEVINKLVDLHEDDPTL
                     GYRFLKDELEHEGIVACENRVHRLCQIGGIQASHAKKKGKAGKPGPPVHDDLLAFEDR
                     HGRIRHRFTAKAPNQVWLTDITEHHTREGKLYLCAVKDLFSNRIVGHSIDSRMKSSLA
                     RAAIRNAITLRNPDGTICHSDRGSQFRSRKVVRLLKNNGLHGSMGRVGAAGDNASMES
                     FYSLLQKNVLNTRTWDTRDQLRHAIVYWIEAKYNRKRRQRALGRLTPVEYETVHQQAT
                     MVA"
     gene            16729..17817
                     /locus_tag="PLJ64_00645"
     CDS             16729..17817
                     /locus_tag="PLJ64_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92031.1"
                     /translation="MARNRMIPYRGFNVQGPSDKNPNYQPNGIHNYNIKDVAKQAGML
                     NADTIRCGMNWAEYEPSPGQYVSSEIDKIKDIVTEGRKGNSNLKVMLQVGNVPWWVPG
                     RVKDPDRPGSANYTTTTEGCHAYGSFCVKVLEELGSYAVAIEHSNEPNGLASADGTDT
                     PPIYYARMAAYAAYWIDFCLGSGYYALVGSLTLSGQWNGLNWQQYLGQVMTEIYRLCR
                     QFYPIDTHPVDFGRLIHSIRISFHPYPYLPAYDFGAALSYVDSKVSWLHSNYPDHKIW
                     ITECGGSSAHMTPAGQTNFLVGMCYQVCGTKPRVEGLIFWPTTDAEGYAWNSTSGFYK
                     FGVYDEDGTTEKQAATFLKTIFADSDWT"
     gene            complement(18714..19022)
                     /locus_tag="PLJ64_00650"
     CDS             complement(18714..19022)
                     /locus_tag="PLJ64_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HNC92032.1"
                     /translation="MATADITVLPIGREGASVSDLLAVIAMHLENQDRVRFEMGPMGT
                     ALEGDPTDIFALAAEIHALPFESGIPRVYTVLKIDQRSDKQQTLESKVQSVRNLLRGS
                     "
     gene            complement(19073..20044)
                     /locus_tag="PLJ64_00655"
     CDS             complement(19073..20044)
                     /locus_tag="PLJ64_00655"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HNC92033.1"
                     /translation="MNTEPAVPAMNGKRRMLIIVNPYATTVSDRLKNLVVYALQGRYE
                     VEVEATESQDHATEIGRDAVDGGYDIVVAFGGDGTVNEVANGLAGTDIPVTFLPGGNT
                     NVVCRSLGIPNDVVDATEHLLSLADDFKPRKIDLGKINDRHFVFACGAGIDATVVEQV
                     DANPKWKRRAGPWFYSWVATKGYFKKYMRNPVQLELEVGGKLHNGVTVLAQNSDPFTY
                     FSRQPVHVCENVSIDDGSLGMILLKRANLLDMGMVVPRLLTEKMRAARHGQVEAFEHF
                     TEARVRSGSKDEDGKVREFPVQVDGDYIGTFEEITLEAAPGALTIIA"
     gene            complement(20061..21080)
                     /locus_tag="PLJ64_00660"
     CDS             complement(20061..21080)
                     /locus_tag="PLJ64_00660"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNC92034.1"
                     /translation="MAEPEPTPESEAALVQAREWGIHTIPVPTPFAVGRINCYLIEGD
                     PLTLIDAGPRSEKSMQGLEEGVEAAGYKLEDIELVVATHQHIDHIGQIHSVVERSGAD
                     VAAIDLVVGRLARFAWGSEQEDQLAVDLMVRYGVPEDIANVLKEVTASFRELGAPVEV
                     NRPFAAGEKITMGSRSYETFHRPGHSPSDTVFWDEERKLMFGGDHLLSHISSNPLISR
                     SLDGSEERTRSLINYRESLRQTAAMPVEIMLSGHGLAITDHAELIEKRIAGQDRRTEK
                     IFGLVAEGHSSPHGIANAIWGDIALTQAYLTLSEVIGHLDILLADGRVVENEGEKYST
                     FEVVS"
     gene            complement(21073..22428)
                     /locus_tag="PLJ64_00665"
     CDS             complement(21073..22428)
                     /locus_tag="PLJ64_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HNC92035.1"
                     /translation="MSIFKAYDVRGLYGEEMDENTAEQIGRAFVLTLADLVRKDPGEL
                     KIGLGRDMRLSAPTMAAAYRDGMISEGAHVIDAGQVATEMLYYLVGSRDLDGGAMCTA
                     SHNPKAYTGVKLVQQGALALSGDAGIGDIRDLVEAGMGIAPGGGSHEEIDLWDEYHRA
                     ALGFIDPSKVRPMRIVVDGGNGMAGPMIGPILKKLGMDLVELYFEPNGEFPDHEPNPL
                     LEENRRLIIDTVKREGADLGIAWDGDSDRCFFIDGDGRFCDGDFICALLARSALETEP
                     GGTILYDPRSSRAVPDVVAAAGGRTDLSRVGHAFFKLRMREENAVFGGEVSGHYYFRD
                     FYCADSGSIPALRLLELLSVDGRSLSELMEEFHSKYFISGEINSEVEDPQAMMDQIEA
                     MHPEAEITHLDGVSIDYEDWHFNVRPSNTEPLLRLNLESLVSKEDMEQKRDEVLAVIR
                     G"
     gene            complement(22468..23001)
                     /locus_tag="PLJ64_00670"
     CDS             complement(22468..23001)
                     /locus_tag="PLJ64_00670"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HNC92036.1"
                     /translation="MAENRIWAPWRLKYVKDADKNNEDQCVFCAKPALGDDETALVVH
                     RGENAFVILNLYPYTNGHLMVAPFEHIGRIQEIPEETTTEMMALAKVAMNRLEDVYGP
                     QGFNVGFNQGRSAGAGVEHHIHMHVVPRWGGDTNFMPVIADHRVMPQSLEDSYRVLRG
                     AFGYEADPADEGTSAAR"
     gene            complement(23114..24130)
                     /locus_tag="PLJ64_00675"
     CDS             complement(23114..24130)
                     /locus_tag="PLJ64_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HNC92037.1"
                     /translation="MPNLPRLRRARESELLSPDSVEPEVLAPRVEEITSGGLRWINIE
                     SPGGEERAWLEENFDFHALDIEDVLSHNQRPKIDEYPDYLFLVVHVPVFDRKAKRLVA
                     GEIDIFVGPDFMVTLPNRGIPPVEYLFERCRADDTLRHKLFARGSGYLMYRLVDDSFD
                     YCFPMLRKIGNKLDALEEEIFEGRSEDVVRDLSNAKQEIINFRKVIRPQRPVLRDLEN
                     VKSRYLASDLDLEIYFDDIVDAHERIWDMLENYKEVVVALEETNESIISHKVNDILRV
                     LTSISVIVLPLTLIASIFGMNVGLPGGGDPATSTDFHFFVVVGIMLVILVGMVAYFRK
                     RDWL"
     regulatory      24249..24362
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            24446..25678
                     /gene="thrC"
                     /locus_tag="PLJ64_00680"
     CDS             24446..25678
                     /gene="thrC"
                     /locus_tag="PLJ64_00680"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNC92038.1"
                     /translation="MAVKNLKCKECSTEYELGANFFCSECFGPLEVKYDFSDLNPEET
                     RRRIQAGSRGIWRYSDFLPFQGKAGDPLEPGMTPLIKADRLSERLGTGAEIFIKNDAA
                     NPTHSFKDRVVAVAIAKAKELGFETVACASTGNLANAVAAHAAAAGLDSYVFVPSNLE
                     EQKLLATDIYGTNLVGVKGNYDDVNRLCTQLADEKPWAFVNVNLRPYYAEGSKTLAYE
                     TVEQLGWNLPDRVVAPIASGSLFTKISRGFQEWKEVGLVEGKQPVFHGAQAEGCSPVA
                     TAFAEGTRVCRPQKPDTIAKSLAIGDPADGPYAVAQANETGGSIDAVSDDEIREGIRL
                     LAESTGIFTETAGGVTVGVLKKLAERGELKDGEKVVVYITGEGLKTLDANRDSFALTE
                     IDPNPDSFESHFGQEVVA"
     gene            25678..25953
                     /locus_tag="PLJ64_00685"
     CDS             25678..25953
                     /locus_tag="PLJ64_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013988830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD family protein"
                     /protein_id="HNC92039.1"
                     /translation="MAVTVKIPTQLRATTGGQADLSAEGATVGEVLEAVFAEHGDLRD
                     RITEDGELRRFVNVYVGGEDIRFGDGLGTAVSDGAEVTILPAVAGGC"
     gene            25937..26677
                     /locus_tag="PLJ64_00690"
     CDS             25937..26677
                     /locus_tag="PLJ64_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNC92040.1"
                     /translation="MPAAAENLKPPVLILSGGRGTRLREQTDSLPKPLVEVGGKPLIW
                     HVIQIYARQGFTRFFPLTGYLGEQIEEFVEGEVWPEGVSVSCLETGLDTPTGGRIAAA
                     SGQVEGTFCATYADGVADLDLDALLDFHRSSGRVATMTAVRPDLQWGVAEIDPAGEVT
                     GFIEKPRSEHWINGGFFCFEPAVFDWLESDSVLEREPLENLAAAGQLGAFRHEGFWDC
                     CDTYKDLVTLNDLWAGGEARWLGSKATA"
     gene            26674..27660
                     /locus_tag="PLJ64_00695"
     CDS             26674..27660
                     /locus_tag="PLJ64_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC92041.1"
                     /translation="MSSSLVTGGQGFIGGHLVRALLERGDEVTVLDLRGAPGHAIELH
                     GLTGEVRLLEGDVADPAAVHRAIRESAAGSSGPDSVFHLAAQTLVGPAAEDPVATFRA
                     NAEGTWVLLEACREAEVGAVVVASSDKAYGPSEALPYREGDELKPAAPYEASKAAADV
                     ISRSYWPAFGLPVGVTRLANVYGGGDLNQSRLVPEVATACLAGRAPQIRSDGSPERDY
                     LHVSDAAAAYLAIEGAVKDGPGRGEAFNAGTGEPWPVRTIVELIADEAAREGAELVEP
                     EFLGDGVPPGEIDRQFVDATKLTELTGWKPEMTLGAGITEAVEWYRNHGNGR"
     gene            27697..28407
                     /locus_tag="PLJ64_00700"
     CDS             27697..28407
                     /locus_tag="PLJ64_00700"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HNC92042.1"
                     /translation="MCGRYTLTSSDQRRLGSRFQISLDDESPGLGRYNVAPTQEVLTV
                     NTHEGSGEREAVASRWGLVPFWAKDLKAGYKMINARNDRLLQSKAYGPLVKKSKNRCL
                     IVADGFYEWLKAEKPKEPRQPVRFTVDGGEPFAFAGLTVSREWEGEPMTSCTIITTDA
                     NDVVSQVHDRMPVILPNEEVEQAWLTGDLSPEEAVSLCVPLDPKRMEAKPANPVLNKV
                     GGAKEGPDFLEAPEKNSD"
     gene            complement(28411..29220)
                     /locus_tag="PLJ64_00705"
     CDS             complement(28411..29220)
                     /locus_tag="PLJ64_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92043.1"
                     /translation="MTDPAPQQTPDESAKRDRIPRLAIAVVVVGLLATAAVYAIAQLP
                     GSGSLEIDWDTTEVVSTPPARDLGNGSFAVARSSLSALAPNDEGGLIYRVAGVVRIDS
                     GGRKLTTVRCDVNSNAAGETNRMAHSSRLRAAWPKPSDALQEQEVPETSFVKFRTPDS
                     KKIDLPIRDVVQRYIDTDAPTQVEWDGYVEDDQNWIWTMPNGTGVGAATLPWLVIFKT
                     DARPKGTIKCKATVGDKSARVLIPFRQDEWPIADDQPNTDEAGTGDVTNVQ"
     gene            29359..30057
                     /locus_tag="PLJ64_00710"
     CDS             29359..30057
                     /locus_tag="PLJ64_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92044.1"
                     /translation="MLIGNQTKARLVATLAGALVALFSLVAVARAIVEQEPNDIRAEA
                     NGPIMDGDTWSGAFGAGGEFGEADWLKFYIAKPGTEVSFDITRGVVLENAANFELTTA
                     SGDSIEGGAAATIPDGGTTGNITKTLSPGKYHFWVVGITDGPDFGMTWQIEAHGDFAT
                     FADIQAACSSGKAKLPNLSQAVASAKKALNKAKKRKKGKAAAVKKAKARLARANKALA
                     SAKSSSAAVCVVEQ"
     gene            30167..31309
                     /locus_tag="PLJ64_00715"
     CDS             30167..31309
                     /locus_tag="PLJ64_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92045.1"
                     /translation="MALTWDQWIDRNRAKFDSDYELDFAAAILSKIPDLAPVSVSCQT
                     WVTQRDGGNRCIDFTIAEGRYVRIAIEVDGYSKDGTPGGQSFEQYLDAHFRDMSLATT
                     TWGIPLHIANRHIRTAPETVRETIDLKLKAQRRLAEALEAGEVDALLRKHAKDALIEE
                     ARSEGELDQTREAKLFELYERGEIESLSNEDQNRRNELRDQIRRLNEELKREKEDRNR
                     AEIERDEAKRGRDETVKENRGMRTGWLISVICLVVGMCLVVALFLLLGGNNDASGSQC
                     DDANAAGDLTLEDVGEIVNVKGQIAQISKVNEATFLNLGGPYGAQDLAVVIWGGNLEN
                     WATPPDVKYDGREVSVTGKVDLYDDPRYGESLEIEANSPNDIAICS"
     gene            complement(31349..31422)
                     /locus_tag="PLJ64_00720"
     tRNA            complement(31349..31422)
                     /locus_tag="PLJ64_00720"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31386..31388),aa:Arg,seq:ccg)
     gene            31461..>32242
                     /locus_tag="PLJ64_00725"
     CDS             31461..>32242
                     /locus_tag="PLJ64_00725"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: protein motif:HMM:NF000801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HNC92046.1"
                     /translation="MDLSVAFLGTGGPVPTALRGTASVLVTRGGEKLMFDCGEGTQRQ
                     MRRSMGLVQVDEIYLTHFHLDHCLGLPGLLKTYDLNDREEPLMLFGPVGLHGLMKDLQ
                     PLIGRLDYHLEVEELEGGDVVPHVDYEIECFPVEHRTRALGYALLEDDRPGRFDPEEA
                     GRLGVKPGPDFKKLQDGEEVDGNDGKVRPDQVMGDDRLGRAVVLTGDTRPCKSTVEAA
                     HDADLLIHDASFMVEDGDRARETGHSTGTEAAEVAAEAGVKLL"
BASE COUNT         6146 a         9967 c        10331 g         5798 t
ORIGIN      
        1 ggtccgtgac gtggcgaccc ggggctggct gccggttccc gtcttctgcg cgggtggcat
       61 cgccaccccg gccgacgccg ccttgatgat gcagctcggt gcggaaggaa acttcgtcgg
      121 ttccggcatc ttcaagtcgg atgacccgga acgccgggcc aaggcgatcg tcgaggcgac
      181 cactcacttc aatgacgcag accgcattgc cgctgcctcc gagcagctcg gcgaagcgat
      241 gccggggatc gagatggccg cactcggcga ggatggccgc ctcgccggac gcggctggta
      301 accaaacgga cccttcaact gacagttccg cccggtccgg tgccgtccgg ccccggatcg
      361 gcgtgctcgc agtccagggc ggcttccaaa ggcacgtcga gcatctgcgc gaggccgggg
      421 cggagccggt cgaggttcga ctgccagccg aactcgaagc gctcgacgga ctgatcctgc
      481 ccggtggcga aagcaccacg atcaccatgg gcatcgagaa agcgggcctc gcaaagggga
      541 tcctcgccca tcaccaggcc ggccggccca ccctcggcac ctgcgccggg atgatcgtcg
      601 ccggccgtga acacctggga ctgatcgaca tcaccacgaa gcgaaacgcc ttcggccgcc
      661 agctttccag cttcgaggac gacatctcgg tcgaaggggt tgtcgacggt ccgattcgcg
      721 cggtcttcat tcgcgccccc tgggtcgaat cgaccgggga gggagtgcag gtactggccg
      781 aggtccgcgg tcacccggtg gcggtcaggc agggaaatgt cacagccctc tcgttccacc
      841 ctgaactgac ccccgacaac cgcatccacc aatggttcaa agaactctgt gttgcctgaa
      901 gccggagcca gcccatccat ctgaagcttc tgtgggctgg cgggtttgac tgggagggcg
      961 ggtcggaccc cgcccgactt gcagttaaga attcgggagg ggtgagccgg cggcgaggaa
     1021 gaaccaggcg atgaaggcga tgaaggcgat cacgaccgag ccgatgatca ctacttcgat
     1081 caacgacgga gggtcgccgc tcttctcgct gtccctgagg ctcttcatcc agcccatccg
     1141 gatcggcccg acatcacggg cgccggtcag ccggatgtag atctcgttca gccggccgag
     1201 gatccaggcg accagcagga tcgaggcgat cgccccgaaa agcacgatca ggtacggccc
     1261 gaggctgggg aactggctgt ccgcgaactg tgatccgacc cacagccaga ccagcggaat
     1321 caccgaccag agcgaaagcg ccgcaagcag catcaccccg gcgaccgcgc ctgccgcgac
     1381 cgtcctcgaa gccctgtgtt tgccctcggg aatcatcagt tgctcttgtt catagaatca
     1441 caacatgtcc ggtcactcga aatggtcttc gatcaagcac aagaaagggg cagctgacgc
     1501 aaagcgcggt cagctcttca ccaaactcgc ccgggcgata accatcgccg cccgcgaggg
     1561 tggtggcgat cccgacggca acccggctct cgccaccgca atccagaagg cccgggcgca
     1621 atccatgccc aaggccaaca tcgagaaggc gatcgaccgg ggcaccggtg aaggcgacga
     1681 cgcggtcgtc gtcgagcgga tccagttcga gggctacgga cccggcggtg ccgcgatcct
     1741 cgttgatgcc ctgaccgaca accgcaaccg gaccagcgcc gaagtcaggc atgccttcac
     1801 caaacacaac ggcagcctcg gcgagcccgg ttcggtcgcc tggatgttcg aaaccttcgg
     1861 tgtggtcgtg gttgacggtg aagccaacag cgaagacgac ctgatggggg cgatcgacgc
     1921 cggagccagc gacgtggtcg aggaagaagg caagctgcgc gtcctcgccg aaccgaccaa
     1981 cctgaccgcg gtcaggcagg caatcgaggc cgacggcatc gagatcgaat cggccgggct
     2041 cgaagtcatc cccaagaaca cggtcgatat cgaggaatcc gacgccggca ccctgatcaa
     2101 gctgctcgaa gcgatcgaag accaggacga cgtgaacgaa gtccacgcca acttcgacat
     2161 cccggacgag gttctggaga agatcgccgc cgattagagt cctcttgcaa agctggaatt
     2221 gatcgaatga tcgctctatc ttcggcccat ggaaagcgaa ggcggtcgct ggagtcgggc
     2281 ctcatgggcg aaaagaatcg gctggatctt cggggggctt gtcgctctct tcctggtcat
     2341 ccagctggtc ccctacggca gggaccacac caatcctccg gtcacccggt caccgaagtt
     2401 cgtcggtgcc cagaccgagc agatcttcaa tgactcctgt ggcgactgcc acagcaacct
     2461 gaccaagtgg ccctggtatt ccaacgtggc gcccgtctcc tggctggtgc agagcgacgt
     2521 tgacgagggt cgttcgatca tgaacttctc cgagtgggac aagccgcagc cggcgatcga
     2581 cgaactggtc gagcagatca atgaaggttc gatgccgcct tccaagtaca cgaggatcca
     2641 cagcggaacg aagctctccg attccgaaaa gcagaccctg atcaacgggc tgatcgccac
     2701 ctaccgggcc aatccacccg gcggcatcaa gggtggctga aagaggcatc cgacgggcgg
     2761 tacagaatca tgagggatga aagtcgtaat ggggatagat cccgggacgg cgaacctcgg
     2821 cttcggcgtg gtcaggatcg agggtggcca catggtcgcc ctggacggcg gggttatcga
     2881 aaccgcttcc ggtgatccgc tcgaacggcg acttggcgag attcattcct ccctttcccg
     2941 cctgatcaag tggcatcagc cggttgccct ggcgatcgag gatctctatt tcggcaagaa
     3001 cgtgggctcg gcgatgaagg tggggcaggc ctccggcgtt tcgatgctgg ccgccgccca
     3061 atgcgacgtg ccctgtttca gctacacgcc gcaggcgatc aagaccgcgg tctgcggttc
     3121 gggcaacgcg gccaaacggc aggtgcagaa gatggttgcg accctgctca acctcagcga
     3181 agttccccag ccggatcacg cggctgatgc cctggcggta gcgatctgcc atggcggtac
     3241 ggctggccgg agggatgcga ccgaggccgc cctggccgga tccgggtcgt ccctgcgggt
     3301 ggcgagttga tcgccacggt ccgcggagag gtcctggtca ggcgctccga ttacgtcgtg
     3361 atcgaggcgg ccggggtggg ataccggctg gcggtcagtt ccgagacgct gcgcagcgtg
     3421 ccggcggccg gccaggagat tttcctccac gccgagacga tcagccgcga cgacagcctg
     3481 ctgctctacg gcttctcatc cgaggaagaa cgggatctct tcaacatgct gatcggggtg
     3541 agcggaatcg gccccaaggt cgcgatctcc gccctttcgg gaggcccgca ccgggaactg
     3601 acccgggcga tcattgccgg tgacgcaaaa cgctttcagg cggtgcccgg gatcggcaag
     3661 cgaaccgccg agcggatcat cctcgaactc aaggacaagg tgacttcggt cgccgaggaa
     3721 gcgggagaag cagttcttcc cgccgcctcg gacgcccgct cgctcgctcg cgacggtctg
     3781 atcaatctcg gttacgcccc gctggaagcc gagcagctgc tcgacggcgt cggcgatggc
     3841 gatgacccgc agcagctgat cggcgcggcc ctcaaacgcg cagccgcgtc cggccggggg
     3901 gagtgagatg agcgacctgc ccgatttcgg tggcaagcgg atcgacctcg gggtcgaggc
     3961 cgacctgccc gaaggtgccg agctgcgggt ccacgacgcc cgggccgatc agcccgacct
     4021 cgagctcgac cagtcgctca ggccgaaagg gctcggggat ttcgtcaacc agaagcaggt
     4081 gaccgaccag ctggccgtgt tcatcgaggc tgcccgccgc cggggggaac cactcgatca
     4141 cgtgcttctg gccggtccgc ccggtctggg caagacctcg ctggccaaca tcgtcgccgc
     4201 cgagatgggc gtgccgctga tccagaccgc cggcccggcc ctggaacgca agggtgacgt
     4261 cgccgccttt ctcacttcgc tggagcccgg cagtgtcttc ttcatcgatg agattcaccg
     4321 gcttgggcgg gccgtggaag agactctcta cccggcgatg gaagacggtg agctgccggt
     4381 ggtgctcggc cagggggccg gcgcccagac cgtgaccctg ccgttgccgc cgtttaccct
     4441 gatcggggcc actacccgca gcggcctgct gacgactccg ctgcgggacc ggtttggcgt
     4501 ctcccatagg ctcgagtact acacaccggc cgatctggcc aggatcgtga ttcgttcggc
     4561 cgggatcctc ggggtcgaga tcgacgaggg gggagcggtg gcgatcgccg agcgttcccg
     4621 cggcacgcca cgggtcgcca accgcctgct caaacgggtg cgggacttcg ccgaggtcaa
     4681 gggctccggt tcggtcgatg cggaaatggc cgacgctgcg ctcgagatgc tcgaagtcga
     4741 caaggccggc ctcgaccggg ccgaccgcgc cctgctcgag ttgatcgcga cgcggtttca
     4801 gggcgggccg gtcggccttt cgacgattgc ggtggcgatc ggtgaggagc aggacacgat
     4861 cgaagacgtg ctggagccct atctgctcca gatgggcatg ctgaagcgca ccccgcgggg
     4921 gcgggttctt acgcagtcag ctttcgagca cctcggcctg ccggttccct cgggccatca
     4981 gtcgctcttc taggtcgctt cgaaaaatcc ctggcgccaa ctggggatcg acggttccca
     5041 tccggtctgc gccttgaact tcgcgttcga tgcgccccgg ccctcggtgg cctgcaacgc
     5101 gatcggtttg ccggcgatca gcgcggccag ccagaacgga accttgcgtg gtggtttggc
     5161 cccgaccgac tctgcgaaaa cgggaatcca gtccctggtc gcggccggtt catcatcggt
     5221 cacgttgtag atcccctcgc ctttttccag ggcagcgacc gcggctgaag ccgcatcttc
     5281 gacgtggagg aaagaggtca tgccgtggcc gccaccgacg atcggcagcc gccgcttgcg
     5341 gacttcaccg gacaggtagc cgtcacgggc gtaccaggtg cccgggccgt agagcagccc
     5401 gtaacggagc acgaccgcct cgaaccggtc gtcttcgagc gactttcgct cgtggccaag
     5461 cacggcgtcg tggaccccgc tgcgatcaac cggttcctcc tcggccttga tcatcggtcc
     5521 gctcagctcg tagaggaagg agatgctctg ggtgaccacg cggggggcgc cggcggcggc
     5581 cgtcgcttcg atcaggttgt ctccgccttc cgacctgatc cggttgttgg cgtcatagaa
     5641 ccccttcttc ttcggctcaa acttcccggg caggctggtc agctggttga ccacgacttc
     5701 gggttcggcc ttgacgacgg cctcgatcag ctgcgcccga tcgaagacgt cgcaggcaac
     5761 cgcgatgccg ccagcctcgc ggatccgcgc cgcgccctgg ccgccgcggg tcgacccgac
     5821 cacctcgtgg cctgccgccg cgagctgacg gaccaggggc aggccgatcg cgccactggc
     5881 tccggcgatg aagatcttca tggacgcagc ctatgtcacc aattcaatac ccttcccgcg
     5941 atgcccttct tcatctgtcc cagttgcggg aaccgcgaga acagcggcga ccggaccgcc
     6001 ggtttcagcc cgcgaccgaa gggctgctcg aagtgcgggt tcgggttcgt cttcgaactg
     6061 ctcgacgatt actacccggc cccgaacgcg gcgttcttcg tctgcgacca ggaagggcgg
     6121 gtcatcgacg cgggccgggg gagtttccag ctgaccggcc tgaccgacga ggacgtgatc
     6181 ggccgcccgg ttcgcgaagt gctggggctc gaatggatcg accccggtga cggcggaccg
     6241 gaagccgaca caaacggtga cggtgagacc gacccgatcg agacttccct ggaatggggt
     6301 gtccgttcgc tcggaaagcg cgtcgaagtg cgctccgaga aggattccgc cgcccaggcc
     6361 attgctgacg tcttcccggc gtatgacgat gacgggggtc tgatgatcgt cttgacgcca
     6421 gaagggtaag cttccgctcg aatgggaggc aggcgccgca acatattcgt gctgctgttc
     6481 gtgctcggtc tggtgatcgt gtcgggcatc gtgtgcctga agaagccgac ccagctcggg
     6541 ctcgaccttc agggcggcat cgagctgacc cttcagggtc ggccgacccc gcaggtcaag
     6601 gaagtcactt ccgaggcgat ggaccgctcg gtggacatca tccgttccgg ttgcgacaag
     6661 ctcggcgttt ccgaaatcga ggtttcgcga ctgggcgccg accagatctc ggtcggcatt
     6721 ccgggcgcga ccaacgtcgg taccgcgacc gaatgcgcaa ccaaacccgc ccggctttac
     6781 ttctatgact gggagaacaa cctggtcgga ccggcccgtg acctcagcct ggtcgggacg
     6841 ggagccgaca ccgcagaagt ctcgaagcag gtccgcaagg cctgggtcga cgccggccgc
     6901 ccgccgaccg aaggcttcaa ccgcacctgg atcggcctcg gtgccgaacc gacgatgtgg
     6961 gacgccgtca acctcgccgc caagcagcca cgcgacgaca agtgcgagga ctgcacctcc
     7021 ggtgacaagt actacctctt cgacgagaag acccatgaac tgatctcggg accggtcacg
     7081 gcgaaggaag acctctacta caacgatcag ggccggccga tccctcacaa gggcatcgtc
     7141 aagaaggttc cccagggcac gatcatcgtc gacgagctgc cgaccgattc cgacggcaag
     7201 acgatcgacg acgagtcgca ggccggctac ttcgtcctca aggacaatgc ggccctttcc
     7261 ggatccgaga tcaaggatcc ccagcagaac ctcgaccagt tcaaccagcc gaatgtgacc
     7321 ttcgacttca ccggcgacgg caaggactcc ttccaggaga tcacccgccg gatcgcccag
     7381 gaagggcagg cccgcgcgat cggaggggtc acctcggccg accaggcttc ggccctttcc
     7441 ggtcacttcg cgatcatcct cgacggccag gtcgtctcga ggccgatcat caacttcgtc
     7501 gagaacccgg acggcatcga cggccgccag ggggcccaga tctccggcgg gttcacggtc
     7561 gaccaggcgc agaacctggc cgagctcctg cagcgtggtg ccctgccgat tgacctcaag
     7621 ctgacttcgc agagccaggt gtcggcgacc ctcgggcagg aagctctcga tcagggcgtg
     7681 ctggcgctgc tggtcgggct cttctgcgtg atcatcttcc tgatcgtctt ctaccgcttc
     7741 ctcggcggtg tcgcctcgct ggcgctgctg gtctacgcgg tcctcttcct cgccctgatc
     7801 aaggtgatcc cgatcaccat gaccctgccg ggcatagccg gtctggtctt aaccatcggg
     7861 gtcgcagccg actccaacat cgtcatcttc gaacgcgtca aggaagagtt gcgtgcagga
     7921 agatccgtgc aatcggcgat tacgctcggt taccggcgcg ggatcagcac gatcatcgac
     7981 gccaacgtcg tcaccctgct gaccgccttc atcctcttcg tcctcgcgac ctcgggcatc
     8041 aagggcttcg ccttcacgct cggtgtcggc accatcgttt cgctcctgac cgcggtcgtc
     8101 ttcacccagg cgctgctcgg ttcgatgtcg aacacgaagc tgatgcgctc cgacaaggca
     8161 ttcgccggca tggagggcaa ggagagatgg catttcgact tcatgggcag cgcgccgaag
     8221 ttctttgcga tctccggcgt gatcctgctg atcggtgccg gcgcgctgac caccaagggc
     8281 ctcaacttcg gcatcgactt cgagtccggc acccggatcg aggtggccct gaacgattcg
     8341 gcctcaaccg aggatgtccg cagcgtgctc agcccgctcg ggctcggcag cgccgacatc
     8401 cagaccatca ccgacaaccc gaccctcggt gacaacgcct tccagatcga gagcgagacc
     8461 ctcgacccgt ccgaggtctc gaaggtcgac gacgcgctga acaaggagtt cggcgtaagt
     8521 gccgacggct tcaacagcac ctcggtcggt ccgacgttcg gcgcctcggt cgcccgcagc
     8581 gcggcccttg cgatcttctt ctcgatgatc ctgatcatgg gatacgtggc gatccgcttc
     8641 ggcggcaagt acacggtccc ggtcctgatc gcgatcgccc acgacttcct gatcgcactc
     8701 ggaatctatg cattgctcgg gttcgaggtc tcgagtgcca ccgtggcggc attcctgacg
     8761 attctgggct actcgcaata cgacacgatc atcgttttcg accgaatccg ggagaacgaa
     8821 ccgaagatgc cgcgggcgac ctactcgcag atcgtcaaca agtcgatgag cgaggtcatg
     8881 acccgttcgc tggtcaccag cttctcgacc gtgatcggcg tcatcgcact gctgatcttc
     8941 ggtggcgagg ttttgcgttc gttcgcgatc gcgatcctgg tcggtgttgc ttccggcacc
     9001 tactcgtcga tcttcatcgc ttcgcccgtg ctcgccctgt ggaaggagca cgagcccgcg
     9061 ttcaagcgtc gccggcgcca gcagatcgag acccagggct cggttcccgc tttcgcatcc
     9121 gacctcgagg tcgcccgcaa ggattcaggc aacggagccg agagcaaggc aggcgaggaa
     9181 ggcgcagtcg aggaagcggc cccgtcgcaa ggcgggccga acctctcgcc ccgtgaccgg
     9241 cgccggctgg agcggcagaa agccaaggaa agcggagggg aagcctgatg tcaatcgtta
     9301 tctggttcac cgtcgcgctc gcgctctggc acttcacgat ctttgtgccc gaccgtttct
     9361 ggcagggcat catcggcgcc ctgatcggct gcgtgctcgg cggattgatc tccggtgcga
     9421 tcgtcgagct gatactcggc aacggccttg ccgacaccga catcatcacc ttcctggccg
     9481 cggtgccggg aacgctgatc ggcgcctggg tgatttaccg gatcggtgtt tcccagggca
     9541 ccgaaccgat tgaagccggc tccgagccgg cggaaaaggt ctagcgggat tcgtttccgg
     9601 gccgagcctt acccggtcga caccgccctc gaactggagc gggcaaccgg gctgagccgg
     9661 cctgtggcga ccgttcttac ccgccgcgga ttcggcgatc cggacgccgc cgcccgcttt
     9721 ctcggggccg gcgaaagcca tgatcccttc gggttcgagg gcatggcaga ggtcgtggcg
     9781 agggtccggg cagcgatcga ggccggcgag aagatcaccg tttacggcga cttcgactgc
     9841 gacggggtct gcgcgacttc gatcctggtc ggtgccctgc gtgaactggg cggtgactgc
     9901 gactggttca tccccgaccg gatcgatgga tacggcctga acccggattc gatccgccgg
     9961 cttcaccagc gcggtacctc gctggtcatc accgtggact gcggcgtgac ctcggtcggg
    10021 gaggtggccc tggccaggga gctgggcctt ggaatcatcg tgaccgacca ccatcaggcg
    10081 gaaacggccc tgccggagtg cccgatcctt cacccggagg tcagtggcta tccgttcccc
    10141 tcgctttgcg gagcggcggt cgccgccaaa ctcgcctcgg cgctcagggc cggcacctcg
    10201 tcgggcccgg agcgcgacga ggcagacctc gatctggtcg ccctcgcgac cgtcgccgac
    10261 gtgatgcccc ttgtcggcga gaaccgtcac ctggtccgcg aaggcgtcag ggtcgcccgt
    10321 cgcgcccggc gggtcggcct ggccgccctg atggccgact gcaggatcga accctcgcga
    10381 ctcagctcgg aggatttcgg cttccggctc ggcccgagga tcaacgccgc cggtcgcctc
    10441 taccgggccg acgccggggt ggagctcttc ctcgcggact cgagggaaag ggccggcgag
    10501 atcgcccgcg agctggccag tgtcaacgcc gaacgacgca cggtcgagcg cgaagtggag
    10561 gggctggcca gggccgagct gaaagcgctc ggcgacccgg gcccggcggt cgtcgtggcg
    10621 ggggagggct ggcatcaggg cgtggtcggg atcgtcgctt cgaaactggt caaggccacc
    10681 ggccggcctt cggtggtgat ctcgatcgac ggtgaaaccg gcaggggttc ggccaggtcg
    10741 gttcccggtc tggaccttca cgccgcgatc ggggagacgg ccgacctgct ggagactttc
    10801 ggcggccacg ccgccgccgc cggtttgagc ataaggaccg accgaattga cgattttcga
    10861 tcggctctgg gccgggcggt cgcgtcacgg atcggcaccg aacccgccga gaaggcgatc
    10921 gagtttgacg ccgtggccgg gggtgccgac ctggggctcg acctggccga ggagatcgag
    10981 aagctgcagc cgttcgggaa cggcaatccg ccggtcaggc ttttcctgcc cggtgccagg
    11041 atcgaggatc tgcaggagat gggcgaaggg aagcactgcc gcttcgccgt ctgttcgggc
    11101 tcccatcgcg caaccggcgt ggccttcggc cggacctcgt tcggactggc agaaggggaa
    11161 agggccgacg tgatcgccga actggggatc aaccactgga acggctcgat cgaaccgcag
    11221 ctgcgggtca ccgaggtttc ccggctcccg gccgccgaac cgctcgcgga gtgtgagccg
    11281 gacgagtggt ggcaacgctt cgagaccgca ctgacgaggc ccctggaggg agccgggccg
    11341 gctgaaaagg cggaggtcag aatcgcctcg gggccgctcg gcctgccggg tgtggcgctc
    11401 ggcgagctga tcagctccgg agagcggatc gtcgtgctca cggccgatgc gcgccagcgc
    11461 tgggtcggtc tcggcggcgc cgccctgggc cgcttttctt cacgcgacgg ggaggaggcg
    11521 gtcggggtct gggccggcag cccggcatcc gtttcggagc cggcccgcga tgcgtcggtc
    11581 ctgctcaccg acttcacgac cctggccggc gccaatcccc cggtgatcga cgacttcgac
    11641 cgcatcgccc tgctcgaccc gccgtatctg gccgcgagcg tggcggaact ggccaggctc
    11701 gacctgccgg ttcatcttct tgccgggccg gtcgagttcg agttcacggc ccgggtcgcg
    11761 gcgtatcacc tcgacccgac cgtgcagctc aggaacctct tccgccgctt tcgcgaagct
    11821 gacgcaatcg acggtcaggc gaccgaaggg gagcccctcc gggcggtcct ctccgaagac
    11881 ggcaactcaa cccgttcgcc ggagcaggcg gcgactctgc tgcggattct ggtcgagaca
    11941 ggcctcgccc gtaccgaagg cgaggggagt gcccgaaggg ccggggtcgt atcctcggtg
    12001 aaggcggatc tttccgtttc ggtcgttttc tcacatcaca tcgagcttca tcaggagcac
    12061 agaggatttc tcagccagtt cgacaggcag acccagacgc agtgacaccg gaggcgacgc
    12121 cgccggtcgg tccggtcgtg cccgcagccg gaaccgtgcc cggaaacgga gccggcattg
    12181 gctccgcagc cggcccgtcg gcgatcgaga ctgccgatct gaccgctcgc gaacgatccc
    12241 tgctcgacga cctgatggcg gtcatccagc agttcgaggc cgacctcgca acccacgggt
    12301 catctcccgc cgcgaccatc gaccgggacg aagtgactcg cgccttcgtc ttcgcctgcc
    12361 ggcaccacgc cgaccagaag cgtaaatccg gcgacgagtt cattacccat ccggtcgggg
    12421 tcgcccgaat ctgcgtcggc atggcgctcg acaccgagac actctgcgcg gccctgctcc
    12481 acgacacggt cgaggacacc tcggccagcc tcgaggagat cgagctggag ttcagcccga
    12541 ccgtttcccg cctggttgat ggcgtcacca aactgaccga gatcaccttc gaaagccgtg
    12601 acgagaggca ggccgagaac taccgcaaga tgatggtggc gatggcgacc gacgtgcggg
    12661 tgatcctgat caagctcgcc gaccgccttc acaacatgcg gaccctgagt tcgctgccca
    12721 agcagaagca gatcctcaag tcccgcgaga cgctggagat ctacgcgccg ctggcccacc
    12781 ggctcggtat ccacgcgatc aagtgggaac tcgaggacct cgccttcgcg acgcttcatc
    12841 cgcgcaagta ctcggagatc aagcacctgg tcgcccagca gcggaccgag cgcgagaact
    12901 tcgtcgagga ggcgggcgag tacctgtcgg acgagctgga gaaggttggc atcgaagccg
    12961 aaatcgccgg ccgcgccaag cacttctact cgatctacac gaagatgacc aagaagggcc
    13021 gggagttcaa cgagatcttc gacctgaccg cgatgcgggt gatcgtcggc tcggtcaagg
    13081 attgttacgg cgcgatcggg gtgatccatt cgctctggaa gcccctgccc ggtcgcttca
    13141 aggattacgt cgcgatgccg aaggcgaaca tgtaccaggc acttcacacg acggtgatcg
    13201 gccttgaggg caagccgctg gagatccaga tccggacggc ggaaatgcat cgtctggcgg
    13261 agtacgggat cgccgcccat gtcgcctaca aggaaggcgg cagcggcacc gattcctccc
    13321 gcgaaaagat gacctggctg cggcagctgg tcgagtcgga gtccgagcag gatccgaccg
    13381 agtttctcca gtcgctgaag gaggatctct tcgaggacga ggtattcgtc ttcactccga
    13441 agggcgaggt gatgaacctc tcggccggct cgaccccggt cgacttcgcg tattcgattc
    13501 acaccgatgt cggccaccgc tgcgtcgggg cgaaggtcaa cggcaaaatc gtgccgctcc
    13561 attaccagct gaagtcgggc gacatcgtcg aagtcctgac cgcgaacaag gatcgcggcc
    13621 cttcccggga ctggctgaag ctggtcggga ccagccgcgc ccgcaacaag atccgcgcct
    13681 ggttccgcga ggaaagccgc gaggacgccg agcgggatgg ccgcgaggca ctggccgaag
    13741 cgctgcgcaa gcgggacctg ccgccgcaca agttctccgg ttccccgatg ctggccgatg
    13801 tgatgcggga gatgggcttc cgcaaggccg aggatttcta catcgccctc ggccagggga
    13861 aggtctcggc caggaccacc gccaacaagc tgatccagcg cctcaggcag ggcgagatca
    13921 tcgaacccgc cccggtcgag aacatcgagt ccggcacccc gaagcgcagc cgcgcgctcc
    13981 gcgaggcatc cgagttcggg atcaaggtcg aggggctcga aaacgtcgcg ctccggctcg
    14041 ccaagtgttg ccggccggtc cccggcgacg agatcgtcgg ttatgtgtcg ctcggccgtg
    14101 gcatcacgat ccaccaggcc aattgccaga acgtggccga tctcgagaag tcaccggaac
    14161 ggatgatcga tgtcgcctgg gagggagaga acgaggcgac ctttcgggtc gagatccaga
    14221 tcgacgccta cgaccggacc cgcctgctcg aggatctttc ccgcaccttc tcggaggccg
    14281 gggtcaacat catctcggcc aacctcacga ccaacccgcc gatggtcaag aaccgtttcg
    14341 tggtggaagt cggagatacc ggccagctca agcaatgcat caaccggatc cgccacgtcg
    14401 aaagcgtctt cgacgcccac cgcgtaaaca ccacggactg accacttccc ggttccaagg
    14461 gcctgtggtc gattaaccct gtactacgag gttaatccgc actcagtgcc tcgcggatgc
    14521 aggccgcgac ccactccgga cgttcagtta cgtccaacca ggtgaatctc aggactttgt
    14581 agccgaggct gcggaggtag cgagtcttct cggaatcggc ttccagagcg aaagcatcac
    14641 cgtagaatct tcggctgtcc agctcgacca ccagccgctc acgcttccag agtgcgtcca
    14701 cttcatatct tgatccattc cgggtttgat ggagacggtt ttcattgggc ggttggtgcc
    14761 gtcaggtagt tgttctcgaa ctctacgggc ggcaggtatt tcagggctcc atggagcctt
    14821 gatcggttgt accagtcacc caggtcaggg ctgccagttc aacgtcggag catttcttcc
    14881 aggggccgtt gcggtcgatt gggtctggcc cgggtttggt tgacacttca agtctgagga
    14941 ttttctctca gtggaaggat gtccgtcatg ccgaagccgt ttccgaggga gttccgtgat
    15001 gatgttgttg ccgtggcccg tacgggccac gcgccgatca agcaggtggc caaagacttc
    15061 gggatctccg aagcgtgcct gcataactgg ttgaagaagg ccgagatcga ggagggaacc
    15121 cggcctggtt tgaccgaggc tgagcgcaaa gagttgcgcg aaacaaaaaa gcggatccgc
    15181 ctgttggagc aggaaaacga ggtgctcaga cgggcggccg cgtatctttc ccaggctaat
    15241 ctgccgggaa aatgatcttc ccgctcgtcc gtgaaatggc tttggctggt gccccgatcc
    15301 gggtgccggt caaggtgacg tgccgggtcc ttgggttcag ctcccagggg tattacaagt
    15361 ggctcaaaga gcctgtttca gatcgggacc gggacgatgc cgaggtcatc aacaagctgg
    15421 ttgacctcca cgaggatgat ccgacgcttg gttaccggtt cctcaaagac gagctggagc
    15481 acgaagggat cgttgcttgc gagaatcggg ttcatcgtct ttgccagata ggcggaatcc
    15541 aggcttccca cgcgaagaag aaggggaagg ccgggaaacc cggcccgccc gttcacgacg
    15601 atcttctggc gtttgaggat cggcatggac ggatccggca ccggttcacc gctaaagccc
    15661 cgaaccaggt ttggttgacc gacatcaccg aacaccacac ccgcgagggg aagctttatc
    15721 tctgcgcggt caaggatctg ttctcgaacc ggatcgttgg tcactcgatc gattcccgga
    15781 tgaagtcgtc gttggccagg gcagcgatcc ggaacgcgat cacgctcaga aaccctgatg
    15841 gaacgatctg ccactccgat cgtggcagcc agttccggtc ccgcaaggtc gttcggctgc
    15901 tcaagaacaa cggcctccac ggttcgatgg gccgggtcgg agccgcagga gacaacgcct
    15961 caatggagtc tttctactcg ctgcttcaaa agaacgttct gaacacccga acctgggaca
    16021 cccgcgatca actccgccac gcgatcgtct actggatcga agcgaaatac aaccgcaaaa
    16081 ggaggcagcg ggccctcggt cggctcaccc cggtcgaata tgaaacagtc caccaacagg
    16141 ccaccatggt ggcctgaaaa cactaaaccc cgagtgacaa cctaactcgg ggcagaccca
    16201 agaggggagt gacgcgatgt cggcgaaacg aatcgaacca gcaggcagac gattctcgcg
    16261 ccgtatggtg ataggtgcga ccgccgcatc tgttctcggt ctcgcgcttg actcaaacaa
    16321 ggtccaggcg gctcaagcaa gaaccgaaca aatatccgct cagaccgtag aacagtccgt
    16381 caagccgctg cttcaatcgt tcgtcgtcac gagaaggtct ccagagttcg atgaactacc
    16441 cccatctctg gtacgactcg ctatcacaga accaaacgtc acctacgact tacatttacg
    16501 cccaaactca cctagcgaag tgcggtctag aactcaattc caaaccaatg aaagcactcc
    16561 caacgcggca attcaagttg cttcctccac attcaagcaa attgtgaatg gaactctgga
    16621 gcttgaaatc gcgctggcac aaaactacgt aattttgagt ggagatcgga gaatattggc
    16681 cccgttcaag agattgtact gttctaagga ttacttcgac acctccggat ggctcgcaac
    16741 cgcatgattc cctatcgagg ctttaacgtc cagggtccga gcgacaagaa tcccaactac
    16801 caaccgaatg gcattcacaa ttacaacatc aaagatgtag ccaaacaagc cgggatgctg
    16861 aacgcagata cgatccgatg cggaatgaac tgggcagagt atgagccaag cccaggccag
    16921 tatgttagct cggaaattga caaaatcaag gatatcgtta cagaaggtcg caaaggaaat
    16981 tccaatctga aggtgatgtt gcaggtcggc aatgtacctt ggtgggtgcc cggacgagtc
    17041 aaagaccctg acagaccggg ctccgctaac tacacaacaa ctactgaggg atgccacgcg
    17101 tacggctctt tctgcgtgaa ggttctcgag gaactaggga gttatgcggt agccatagaa
    17161 cattccaacg aaccaaacgg gctggcttca gcagacggaa ctgacactcc acccatctat
    17221 tacgccagaa tggccgcata tgcggcgtac tggatagact tctgcctcgg atctggctat
    17281 tacgcgctcg tgggttcttt gaccttgagc ggacaatgga atggcttaaa ttggcaacag
    17341 tacctcggtc aggtaatgac tgagatctat cgcttgtgtc ggcagttcta ccctattgac
    17401 acccatccgg tggattttgg tcgccttata cactccatcc gaatatcatt tcacccctac
    17461 ccgtatctac cagcttatga ttttggggct gctttgagct atgtagattc gaaggtttca
    17521 tggctacatt ccaactatcc agatcataag atttggataa ccgaatgtgg tggatctagc
    17581 gcccatatga cccccgctgg gcaaactaac ttcctagttg gaatgtgtta ccaggtctgt
    17641 ggaacaaagc ccagggtgga aggtctaata ttctggccaa ccactgatgc cgaaggatac
    17701 gcttggaact caacctcagg attctacaag tttggtgtgt acgatgaaga tgggacgacg
    17761 gagaagcagg cagcaacatt tttgaagacc atatttgcgg acagcgattg gacatgaccg
    17821 aaactttgtc ccaactgatg tgtttaagct ttgcgtagcg ctcgtttcgt gattgaaagg
    17881 atggctggtt cgtttgcaaa ggtttaaact cacaccctcc ctgttcgtta gtttgatcgc
    17941 cctcttcgtc gccttgggcg gtggtgccta tgctgcgatt gggtttcact cgattggttc
    18001 agtccaactg aagtccggag cggtcaccaa gcgagtcttg cgaaatgatt ccgtcgtgca
    18061 ggccaagatt cgtccgggat cagtaggggc tgcccaaatt cggtctcaaa gtgtgaatgc
    18121 aagcaaggtt cgggggagtt tgccaggcac attggtagca tacgccaagg tgacggctga
    18181 aggcaaggtc gatcccgacg agtcatggcg tatatccgac gagaatgttc tccaaatgcc
    18241 catcgcgaat tattgtcttc gaaatttgcc tgctcataga accgcgatgg tgacccctgg
    18301 ctttgacgga agcggatcag ttgttgcaaa cttgagtcaa tcggtaccgc cagagtcaat
    18361 ctgtgatcaa gtgagtggca accctattca ggtctcaacc accttcgcga tcgaagagaa
    18421 ccttaccgcg tggaagtacg agcctttcta cgtgtaccta tttaagtagc agtgccttca
    18481 atcctgtctt gaagtgctgc acgtttcggg caggttctcc gatccgtctc gcggatctca
    18541 tttccaattc cgatttcagc acagcggctc gcggtgtgtc gaaattggtt ggtcgtttga
    18601 ccgccatctc ctcggctagg cgtcccggga tgaatcgctt ggcgacgaca attaagccgg
    18661 gggcggttca gcccgtgggt cgattaacca agtactacga ggttacccag ccctcagcta
    18721 cctcgcagca ggttgcggac cgattggacc tttgactcga gggtttgctg cttgtcgctg
    18781 cgctgatcga tcttgaggac cgtgtagacg cgggggatgc ctgactcgaa ggggagggcg
    18841 tggatctcgg cggcgagggc gaagatgtcg gtcggatcac cttccagtgc ggtgcccatc
    18901 gggcccatct cgaaacggac cctgtcctga ttctccagat gcattgcgat caccgcgagg
    18961 agatccgaaa ccgaggctcc ctcacgcccg atcggcaaga ccgtgatgtc agcggtagcc
    19021 atgacctgat ggtaaccgga ggcgcaggcc ggccttccgg gccggggacc ggtcaggcga
    19081 tgatggtgag ggcgccgggg gctgcttcga gggtgatctc ctcgaaggtg ccgatgtaat
    19141 cgccgtcaac ctggaccggg aactcgcgaa ccttcccgtc ctcgtccttt gatcccgagc
    19201 gcactctggc ttcagtgaaa tgctcgaagg cttcgacctg cccgtgtctg gcggcccgca
    19261 tcttctcggt cagcaaccga gggacaacca ttcccatgtc gagcaggttg gcgcgcttca
    19321 acaggatcat gcccagggat ccgtcatcga tcgagacgtt ctcgcagacg tgaaccggct
    19381 gccggctgaa gtaggtgaat ggatccgaat tctgggccag cacggtcact ccgttatgga
    19441 gctttccccc gacttcgagt tcgagctgga ccgggttgcg catgtacttc ttgaagtaac
    19501 ccttggtcgc gacccaggag taaaaccacg gaccggctcg ccgcttccac ttcggattcg
    19561 catcgacctg ctcgaccacg gtcgcgtcga tccccgcccc gcaggcgaag acgaagtggc
    19621 gatcattgat cttgccgaga tcgatctttc ggggcttgaa atcatcggcc agcgaaagca
    19681 ggtgctcggt tgcgtcgacc acgtcgttgg ggatcccgag cgaacgacag accacgttgg
    19741 tgttgccgcc gggcaggaag gtgaccggga tgtcggtacc ggccaggccg ttggcaacct
    19801 cgttcacggt gccatcaccg ccgaaagcga ccacgatgtc gtagccgccg tcgaccgcgt
    19861 cgcggccgat ctccgtggcg tgatcctggc tttcggtcgc ttcgacctcg acctcgtatc
    19921 ggccctgcag ggcgtagacg acaaggttct tgagccggtc tgaaaccgtc gtcgcgtagg
    19981 ggttgacgat gatcagcatc cgtcgtttgc cgttcatcgc gggaactgcc ggttcggtat
    20041 tcaccgtcgc acctcggaga tcacgaaacc acctcgaagg tggagtactt ctcgccctcg
    20101 ttctcgacca cgcggccgtc ggcgaggagg atgtcgagat ggccgatcac ttcggagagg
    20161 gtgagatagg cctgggtcag ggcgatgtca ccccagatcg cgttggcgat gccatgcggc
    20221 gagctgtggc cttcggcgac caggccgaag atcttttcgg tccggcggtc ctggccggca
    20281 attcgcttct cgatgagttc ggcgtgatcg gtgatcgcca ggccgtggcc ggaaagcatg
    20341 atctccaccg gcatggcagc ggtctgccgc agcgattcgc ggtagttgat caaggaacgg
    20401 gtgcgctcct cgctgccatc cagggaacgg gagatcagcg ggttggagga gatgtgggag
    20461 agcaggtgat cgccgccgaa cataagtttc cgctcttcgt cccagaagac ggtgtccgac
    20521 gggctgtggc cggggcggtg aaaagtctca tatgaccggg atcccatcgt gatcttctcg
    20581 ccagcagcga aaggacggtt cacttcgacc ggggcgccga gctcgcgaaa gctggcggtg
    20641 acttccttca ggacattggc gatgtcttcc gggactccgt agcggaccat cagatcaacc
    20701 gccagctggt cttcctgttc ggagccccag gcgaacctgg cgaggcgacc gaccacgagg
    20761 tcgatcgccg ccacatcggc gccggatcgc tcgaccaccg aatggatctg gccgatgtgg
    20821 tcgatgtgct gatgggtggc aaccacaagt tcgatgtctt cgagcttgta gccggccgct
    20881 tcgacccctt cttcgagtcc ctgcatcgat ttctccgaac gagggccggc atcgatcaga
    20941 gtcagcgggt ccccctcgat caggtagcag ttgatgcgac cgaccgcgaa gggggtcggg
    21001 accggaatcg tgtggatgcc ccattcacgg gcctggacca gggcggcttc cgactcgggt
    21061 gtcggttcgg gttcagccac ggatcaccgc gagcacttcg tctcgtttct gctccatgtc
    21121 ttccttcgac accagcgact cgaggttgag cctgagcaga ggctcggtgt tggaggggcg
    21181 gacgttgaaa tgccagtcct cgtaatcgat cgaaacgcca tcgagatgag tgatttcggc
    21241 ctcgggatgc atcgcctcga tctggtccat catcgcttgg ggatcttcaa cctcggagtt
    21301 gatctcaccc gagatgaagt acttcgagtg aaattcctcc atcagctccg aaagcgagcg
    21361 accgtcaacc gaaagaagct cgagcaggcg cagggccgga atcgagccgg aatcggcgca
    21421 gtagaagtcg cggaagtaat agtgcccgga gacttcgccg ccgaagacgg cattctcctc
    21481 gcgcatgcgt agtttgaaga aggcgtgacc aacccgggaa aggtcggtcc gtccgccggc
    21541 ggcggccaca acatccggca ccgcccggct ggaacggggg tcgtagagga tggtgccgcc
    21601 gggctcggtc tccagggccg agcgggccag aagcgcgcag atgaagtcac catcgcagaa
    21661 ccgcccgtcg ccgtcgatga agaagcagcg gtcggagtcg ccatcccagg cgatgccaag
    21721 gtcggctcct tcccgcttga ccgtgtcgat gatcagccgg cggttttcct ccagcagcgg
    21781 attcggttcg tggtcaggga attccccgtt cggctcgaaa tagagctcga ccaggtccat
    21841 gccgagcttc ttcaggatcg gcccgatcat cggtccggcc atgccgttgc cgccgtcgac
    21901 cacgatccgc atcggcctga ccttcgaggg atcgatgaag ccgagcgccg cccggtgata
    21961 ctcgtcccag agatcgatct cttcgtggct gccgccaccg ggagcgatcc ccatgccggc
    22021 ttcgaccagg tcgcggatgt ccccgatccc ggcgtcaccg gaaagtgcca gcgctccctg
    22081 ctggaccagc ttgacgccgg tgtaggcctt gggattgtgg gaggcggtgc acatcgcacc
    22141 gccgtcgagg tcccggctgc cgaccaggta gtagagcatt tcggtcgcga cctggccggc
    22201 gtcgatcaca tgagcgccct cgctgatcat gccgtcccgg taggcggccg ccatggtcgg
    22261 agctgacagc cgcatgtcac ggccaagccc gatcttgagt tcccccggat ccttccgcac
    22321 gaggtcggcg agggtgagga cgaaggcgcg gccgatctgc tccgcggtgt tttcgtccat
    22381 ttcctcgccg tagagcccac ggacgtcgta tgccttgaaa atggaaatgg tgctgccttt
    22441 cctgccttgt ttgactgggg gactgggtta cctggcggcg gacgttccct cgtccgccgg
    22501 gtcggcctcg tagccgaagg cgccgcgcaa cacgcggtag ctgtcctcga gagattgcgg
    22561 catgacccgg tgatcggcga tcaccggcat gaagttcgtg tcgccgcccc agcgcgggac
    22621 gacatgcatg tggatgtgat gctcgacccc ggctccggcg gagcggccct ggttgaaacc
    22681 gacgttgaat ccctgcggtc cgtagacatc ctcgagccgg ttcatcgcca ccttggcaag
    22741 ggccatcatc tcggtggtcg tctcttcggg gatctcctga atgcggccga tgtgttcgaa
    22801 gggggcgacc atcaggtgcc cgttcgtgta cgggtagagg ttgagaatca cgaaggcgtt
    22861 ctcgccgcgg tggacgacca gcgcggtttc atcgtcaccg agcgccggct tggcgcagaa
    22921 aacgcactgg tcctcgttgt tcttgtcggc gtccttcaca tacttgaggc gccagggtgc
    22981 ccaaatccgg ttctctgcca tcggcgcccc atcctattgg tccaacgcga gtcggagtcc
    23041 gatcatctat tcgctttctc tgtgatcgga cgggtggaac ttggaggcgg ggcgaagctc
    23101 gccgactact tattcagagc cagtctcgct tgcggaagta ggcgaccatg ccgaccagga
    23161 tcaccagcat gatccccacc accacgaaga agtggaaatc ggtactggtg gccgggtcgc
    23221 cgccaccggg caggcctacg ttcatgccga agatcgaggc gatcagggtc aagggcagga
    23281 cgatcaccga gatcgaggtg agcacccgca ggatgtcatt gaccttgtgg gagatgatcg
    23341 attcgttggt ctcctcgagc gcgaccacga cctccttata gttctcgagc atgtcccaga
    23401 tccgttcgtg ggcgtcgacg atgtcgtcga agtagatctc gaggtcgaga tcactggcca
    23461 ggtagcggga cttgacgttc tcgaggtcac gcagaaccgg tcgctgcggc cggatcacct
    23521 tgcggaagtt gatgatctcc tgcttggcgt tcgagaggtc gcgaaccaca tcctcggagc
    23581 ggccctcgaa aatctcttcc tcgagcgcat cgagtttgtt gccgatcttt cgcagcatcg
    23641 ggaagcagta atcgaagctg tcatcgacca ggcggtacat caggtaaccg gaaccgcggg
    23701 cgaagagttt atggcggagg gtgtcatcgg cccggcagcg ctcgaagagg tactcgaccg
    23761 gcgggatccc gcggttcggc agggtgacca tgaaatcggg tccgacgaag atgtcgattt
    23821 cgccggcgac caggcgcttg gccttgcggt cgaaaaccgg tacatggacg acgaggaaga
    23881 ggtagtccgg gtattcgtcg atctttggtc gctggttatg cgaaagcacg tcctcgatgt
    23941 cgagggcgtg gaaatcgaag ttctcctcca gccaggcgcg ttcctccccg ccagggcttt
    24001 cgatgttgat ccagcgcaaa ccgcccgagg tgatctcctc gacccgcggg gcgaggacct
    24061 cgggttcgac tgaatccggg ctcagcagct cagattcgcg cgcacggcga agacgaggga
    24121 ggttcggcat cgccgcctcc attcatgtca attcgcttcg cgggcatgaa acaaccctag
    24181 accagcagtc aaacccccgc gtaaacggga tgtttatgca tgctggtaac ctgtcgccag
    24241 acataagcgc cacatccaga ggggtggagg gacatggccc tacgaaaccc cggcaaccag
    24301 cggaagcgag agctttcgag aaggtgccaa atccagcggc tcaagtagcc gggagatgtg
    24361 gggagaagtc cgatccggcc ttcgccccac gggcgaaggt tttttgtttc cccggcaggg
    24421 gaaagaacga aaggtaagag atcggatggc agtaaagaac ctcaaatgca aggaatgttc
    24481 gaccgagtat gagctcggtg cgaacttctt ctgctccgaa tgtttcgggc cgctggaagt
    24541 caagtacgac ttctcggacc tgaacccgga agagacccga aggaggatcc aggcgggatc
    24601 gcgcggaatc tggcgctatt cggacttcct gcccttccag ggcaaggccg gcgacccgct
    24661 cgagccgggc atgactccgc tgatcaaggc ggaccgcctc tcggagcgcc tgggaaccgg
    24721 ggcagagatc ttcatcaaga acgatgccgc caacccgacc cattccttca aggaccgggt
    24781 cgtcgccgtg gcgatcgcca aggcgaagga gctcggcttc gagaccgtcg cctgcgcctc
    24841 gaccggcaac ctggccaacg cggttgcggc tcatgccgcc gcggccggac tcgattccta
    24901 tgtctttgtt ccgtccaacc tggaagagca gaagctgctg gcgaccgaca tctacggcac
    24961 caacctggtc ggcgtcaagg gcaactacga cgatgtgaac cggctctgca cccagctcgc
    25021 ggacgagaag ccctgggcct tcgtcaacgt gaacctgcgc ccgtactacg ccgagggctc
    25081 gaagaccctt gcctacgaga cggtcgaaca gctcggctgg aacctgcccg accgtgtggt
    25141 cgctccgatc gcgtcgggtt cgcttttcac caagatctcc cgcggcttcc aggagtggaa
    25201 ggaagtcggc ctggtcgaag gcaaacagcc ggtcttccac ggcgcccagg ccgagggttg
    25261 ttccccggtc gccacggcct ttgccgaagg cacccgggta tgccgtccgc agaagccgga
    25321 cacgatcgcc aagagcctcg cgatcggtga cccggccgac ggtccctacg cggtcgccca
    25381 ggcgaacgaa accggcggtt cgatcgatgc ggttagcgac gacgagatcc gcgagggtat
    25441 ccgcctgctg gccgagtcga ccggcatctt caccgagact gccggcggcg tcacggtcgg
    25501 cgtgctgaag aagctggccg agcgcggtga gctgaaagac ggcgagaagg tcgtcgttta
    25561 catcaccggc gaggggctga agaccctcga cgccaatcgc gatagctttg cgctgaccga
    25621 gatcgatccg aatccggact cgttcgaatc ccacttcggc caggaggtcg tcgcctgatg
    25681 gcagtcacag tcaagattcc gacccagctt cgcgccacca ccggaggcca ggccgacctg
    25741 agcgccgagg gtgccacggt gggcgaggtc ctcgaagcgg tcttcgccga gcacggtgac
    25801 ctccgagacc ggatcaccga agacggcgaa ctgcgccgtt tcgtcaacgt ctacgtcggc
    25861 ggtgaagaca tccgcttcgg cgacgggctc ggcaccgcgg tttccgatgg tgccgaagtg
    25921 acgatccttc cggccgttgc cggcggctgc tgaaaacctg aagcccccgg ttctgatcct
    25981 cagcggcggc cggggcacgc ggctgcgcga gcagaccgat tccctgccca agccgctggt
    26041 cgaggtcggt ggcaagccgc tgatctggca tgtgatccag atttacgccc ggcagggatt
    26101 caccaggttc ttcccgctga ccggttacct cggcgagcag atcgaggagt tcgtcgaagg
    26161 cgaagtctgg ccggagggcg tttccgtgag ttgcctggaa accgggctcg acaccccgac
    26221 cggcggtcga atcgccgcgg caagcggtca ggtcgaggga accttctgtg cgacctacgc
    26281 cgacggtgtc gccgacctcg acctcgacgc acttctcgac tttcaccgct ccagcggcag
    26341 ggtggcaacc atgaccgcgg tccggccgga cctgcagtgg ggcgtggcgg aaatcgatcc
    26401 ggccggcgag gtcaccggct tcatcgagaa gccgaggtcc gagcactgga tcaacggcgg
    26461 ctttttctgt ttcgaacctg ccgtcttcga ctggctcgag tcggactcgg tcctcgagcg
    26521 cgagccgctt gagaacctgg ccgcggcagg gcagctcggt gccttccgtc acgaagggtt
    26581 ctgggactgc tgtgacacct acaaggacct ggtcaccctg aacgatctct gggctggtgg
    26641 cgaggcacgc tggctcggct cgaaggcaac cgcttgagtt cctcgctggt caccggcggc
    26701 caggggttca tcggggggca cctcgtccgg gcgctgctgg agcggggcga tgaggtcacg
    26761 gtgctggacc tgcgtggcgc tcccggccat gcgatcgagt tgcacggact gaccggcgag
    26821 gtgcgactcc tcgaaggcga cgtcgccgac ccggctgccg ttcatcgcgc gatccgcgaa
    26881 tcggcggccg gttcgtcggg gccggattcg gtctttcacc tggccgccca gaccctggtc
    26941 gggccggcgg ccgaggatcc ggttgcaacc ttccgcgcca acgccgaggg aacctgggtg
    27001 ctgctcgagg cctgccgcga ggcggaggtc ggcgcggtcg tcgtcgcctc ctccgacaag
    27061 gcctacgggc caagcgaggc gttgccctac cgcgagggag acgagctgaa gcctgccgct
    27121 ccctacgaag ccagcaaggc cgcggctgac gtgatcagcc gcagctactg gcccgccttc
    27181 ggcctgccgg tcggggtgac ccgcctggcc aacgtctacg gcgggggaga cctgaaccag
    27241 tcacggctgg tgcccgaggt ggcgaccgcc tgcctggccg ggcgcgcgcc gcaaatccgc
    27301 tccgacggct cgcccgaacg ggactacctg catgtcagcg acgcggccgc cgcctacctg
    27361 gcgattgaag gtgcggtgaa agacggtccc ggtcgcggcg aagccttcaa cgccggaacc
    27421 ggcgaaccct ggccggtacg gacgatcgtc gagctgatcg ccgacgaagc cgcccgcgaa
    27481 ggtgccgaac tggtcgagcc cgaattcctg ggtgacggcg tgcccccggg tgaaatcgac
    27541 cgccagttcg tcgacgcgac caaactgacc gaactcaccg gctggaagcc cgagatgacc
    27601 ctcggcgccg gaatcaccga agcggtcgag tggtacagga accacgggaa tggccgttga
    27661 catgcgggcg atttccgccc tgttaggttg ggggccatgt gcggcagata cacactgact
    27721 tcatccgatc agcggcggct cggaagccgg ttccagattt cacttgatga cgagtcgccc
    27781 ggtctcgggc gctacaacgt ggcgccgacc caggaagtgc tgaccgtcaa cacgcacgaa
    27841 gggtccggcg agcgcgaagc ggttgcctcg cggtggggtc tggttccgtt ctgggccaag
    27901 gacctcaagg ccggctacaa gatgatcaac gcccgcaacg accgtttgct tcagagcaag
    27961 gcctacggtc cgctggtcaa gaagagcaag aatcgctgcc tgatcgtggc cgacggtttc
    28021 tacgaatggc tgaaggcaga gaagccgaag gagccgcgcc agccggtccg tttcacggtc
    28081 gacgggggag agcctttcgc tttcgccggt ttgacggttt cccgggagtg ggaaggggag
    28141 ccgatgacct cctgcacgat catcaccacc gacgccaatg acgtggtcag tcaggttcac
    28201 gaccggatgc cggtgatcct gccgaacgaa gaggttgagc aggcctggct gaccggtgac
    28261 ctttctcccg aggaggcagt ttcgctctgc gtgccgctcg acccgaagcg gatggaggcg
    28321 aaaccggcca acccggtgct gaacaaggtc ggcggggcaa aagaaggccc cgacttcctc
    28381 gaggccccgg aaaagaactc cgactagccg ctactggacg ttggtgacgt cgccggtgcc
    28441 ggcctcgtcg gtgttcggct gatcgtcggc gatcggccat tcgtcctgac ggaaggggat
    28501 caggacccgg gccgacttgt caccaacggt ggctttgcac ttgatcgttc ccttggggcg
    28561 ggcatcggtc ttgaagatga ccagccaggg cagcgtcgcg gcaccgaccc cggttccgtt
    28621 cggcatcgtc cagatccagt tctggtcgtc ttcgacgtat ccgtcccact cgacctgggt
    28681 cggagcgtcg gtgtcgatgt acctctgaac gacatcgcga atcggcaggt cgatcttctt
    28741 tgagtcaggg gtgcggaact tgacgaaact cgtttccgga acttcctgct cctgaagggc
    28801 gtcgctgggc ttcggccagg cggcccgcag gcggctcgag tgagccatcc ggttcgtttc
    28861 gccggcagcg ttcgaattga cgtcgcagcg gaccgtggtc agtttgcgtc cgccggaatc
    28921 gatccggacc acgccggcca cccggtagat gagtccgccc tcgtcattgg gcgccagcgc
    28981 ggaaaggctg cttctggcca ccgcgaacga gccgttgccg agatccctgg cgggtggtgt
    29041 gctgaccacc tcggtggtat cccagtcgat ttcaagcgag ccgctgccgg gcagctgcgc
    29101 gatcgcgtag acggcggccg tggccagcag gccgaccacg accacggcga tcgccaggcg
    29161 aggaatgcgg tcacgcttcg cggactcgtc gggtgtctgt tgaggggctg ggtcagtcat
    29221 cggacggcgg tgcggaggat attcgatgcg gcgcggccgg cagcgcgtcg ggacggttcc
    29281 ggcctcaagg gttgcgcggc tttacataac ccggttaggg tgggaatgtc gagctgaaac
    29341 cgggatttgg gggatcaaat gctcatcgga aatcagacga aggcgcggct tgtcgcaacg
    29401 ctcgctggcg ctcttgtcgc actcttcagt cttgtcgccg tcgccagggc gatcgtcgag
    29461 caggagccga acgacattcg agccgaagca aacggaccga tcatggacgg tgacacctgg
    29521 tccggtgcct ttggcgcggg tggcgagttc ggagaagccg actggctgaa gttctacatc
    29581 gcaaaaccgg ggacggaggt gagcttcgac atcacgcgcg gggtcgtgct ggagaacgca
    29641 gcgaatttcg agctcacgac cgcgagtggt gactcgatcg agggcggcgc cgcggcgacc
    29701 attccggacg gcggcactac cggcaacatc accaagaccc tgagtccggg caagtaccac
    29761 ttctgggtgg tcggcataac ggacggaccc gatttcggga tgacatggca gatcgaggcg
    29821 cacggcgact tcgcgacctt tgccgacatc caggcggcgt gcagttcggg caaagcgaag
    29881 ctgccgaacc tcagccaggc cgtagccagc gcgaagaagg ccctgaacaa ggcgaagaaa
    29941 cgcaagaagg gcaaggccgc tgcggtaaag aaggcgaagg cgaggctggc cagggcgaac
    30001 aaggcacttg cgagcgccaa aagctcatcg gctgcggtct gcgtggtgga gcagtagcca
    30061 accggaagta ttctccgcca caacgagacg tgggtgtcag gcagtccctc ggccagttgg
    30121 ccgtaaatcc cgtacgagaa cgaaccaagc aatagggttc cgaggcgtgg cactcacttg
    30181 ggaccaatgg attgatcgca atcgggcaaa gttcgatagt gattacgaac tagactttgc
    30241 cgctgcgatc ctgagcaaaa ttccggacct cgcgcccgtt tctgtcagct gtcaaacctg
    30301 ggtcacgcaa cgagacggcg gaaatcgttg catcgatttc actatcgccg agggaagata
    30361 tgtgcgaatt gccattgagg tggatggcta cagcaaggat ggcacaccgg ggggacagtc
    30421 gttcgagcag tatcttgacg cccattttcg cgacatgtcg ttggcgacga cgacctgggg
    30481 aatcccattg cacatcgcta atcgccacat caggacagct ccggaaacgg tccgagagac
    30541 catcgatcta aagctgaagg cccagcggcg cctcgccgaa gcacttgagg ctggagaagt
    30601 cgacgctcta ttgaggaaac acgccaagga cgctctgatc gaggaagcca ggtcagaagg
    30661 ggaattggat caaacaaggg aagccaagct gttcgagctg tacgagcgcg gcgaaatcga
    30721 aagtctctct aacgaagatc aaaatcgccg aaacgagctc cgagaccaga ttcgacggct
    30781 gaatgaggaa ctgaagagag agaaggaaga tcggaaccgt gctgagatag aacgggacga
    30841 ggccaagcga ggacgagacg aaacggtgaa ggagaatcgc ggaatgagaa ccggctggct
    30901 catctcggtc atttgtctgg ttgtaggtat gtgcctggtg gtggctcttt ttctcttgct
    30961 agggggaaat aacgacgctt cggggtctca atgcgatgac gcgaacgcgg caggcgatct
    31021 tacccttgaa gacgtgggcg agatagtgaa cgtgaaggga caaatcgctc agatatccaa
    31081 agtaaatgaa gctacgttcc tgaatctcgg aggaccgtat ggcgcccaag atctggcggt
    31141 ggtgatctgg ggaggtaatc tcgaaaactg ggctacgcca cctgatgtga agtacgatgg
    31201 gcgtgaagtg tcggttacag gaaaggttga tctctacgac gatcccagat acggagagtc
    31261 acttgagata gaggccaatt cacctaacga catagcgatc tgttcgtaaa gatccggtca
    31321 aaaatcagaa actggccata tcggaagacg cgcccggcag gattcgaacc tgcgacctca
    31381 tgctccggag gcatgcgctc tatccagctg agctacgggc gcggaagcgc tgagtctagt
    31441 gatcgggcta gggtgcgggc atggacctct ccgtcgcatt tcttggcacc ggtggcccgg
    31501 tgccgaccgc cctgcggggc accgccagcg tgctcgtcac gcggggcgga gaaaaactca
    31561 tgttcgactg cggcgaggga acccagcggc agatgcggcg atcgatgggg ctggtccagg
    31621 tcgacgagat ctacctgact cacttccacc tcgatcactg cctcggcctg cccggtctgc
    31681 tcaagaccta tgacctgaat gaccgcgagg agccgctcat gctcttcggc ccggtcggcc
    31741 ttcacgggct gatgaaggac ctgcagccgc tgatcggccg gctcgattac cacctcgagg
    31801 tcgaggagct cgaaggcgga gacgtggttc cccacgtcga ttacgagatc gagtgtttcc
    31861 cggtcgaaca ccgcacccgg gcgctcggct acgccctgct cgaggatgac cggcccggcc
    31921 gattcgaccc cgaagaagcc ggccggctcg gcgtcaaacc cggccccgat ttcaagaagc
    31981 tccaggacgg agaagaagtt gacggaaacg acgggaaggt ccggcccgac caggtgatgg
    32041 gtgacgaccg gctcggccgg gcagtcgtac tgaccggtga cacgcggccc tgcaagtcaa
    32101 ccgttgaagc tgcgcatgac gccgatctgc tgatccacga cgccagcttc atggtcgagg
    32161 acggtgaccg ggccagggag accggccatt cgaccgggac cgaagcggcc gaggtcgcgg
    32221 ccgaggccgg ggtcaagttg ct
//
LOCUS       DAOQKN010000005        30623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_588_length_30623_cov_5.889329, whole genome shotgun sequence.
ACCESSION   DAOQKN010000005 DAOQKN010000000
VERSION     DAOQKN010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 30623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30623
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_588_length_30623_cov_5.889329"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="PLJ64_00730"
     CDS             <1..291
                     /locus_tag="PLJ64_00730"
                     /inference="COORDINATES: protein motif:HMM:NF019614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HNC92047.1"
                     /translation="VIVRTAAEMRAVELANPFKSAAYNRIGVLFLDHRLPRENIRKAK
                     GRKNEEIEIGKREIYVHYPDGMGRSKLNLPAAKYGTTRNLNTVSKLADMAGG"
     gene            complement(292..1074)
                     /locus_tag="PLJ64_00735"
     CDS             complement(292..1074)
                     /locus_tag="PLJ64_00735"
                     /inference="COORDINATES: protein motif:HMM:NF018031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNC92048.1"
                     /translation="MAGPHAINGFDRTLSRIGTASADGYGIIVRRLKGPGDGWFSASE
                     LDGSGRVQALLLSRISGMAGPPEDHLRAEWLLESLARAIADLGGSFIVAAGQLPELTP
                     ENILLAAEGGLFRATAVASDRGLETTDPQSLATAAGPSDLDVLAEAFRRGFADLLAPT
                     VDWLDAQGLRPAKTLWHAAADRLAQALVWSGKAFDRPGLALELTRLTVGPDCPDPRLA
                     IPIERAEDDWGDEYHLRSTCCLAYRTEGGQLCQSCPLVKDRD"
     gene            complement(1079..2011)
                     /locus_tag="PLJ64_00740"
     CDS             complement(1079..2011)
                     /locus_tag="PLJ64_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92049.1"
                     /translation="MLRPLKTSLGLIAFACVLILPNFAQAFDAGVRTPLLTDRSPGAV
                     SVPFEIPVRQSAAVPQPLDFEIAVEGFRLDPAPAADLRIGSLDMVTDAGDFDNKAIYS
                     NGPAIDGARTWTLDWQLSNHPITATVEDGTGLGPAGEKTVDDGSTLISFTVPGNYHGF
                     RLMGLSLRFNQQEKGIPTPGIGAVNPTAPGSYLIRSRIESVAPGSSVKVASTRVRIGP
                     DETRPKTRLAVGANRSRVRPGGLVRFSIRTLNGTSDFVTVRRGRHEIGELKAGPAVRY
                     LYWRASRRLRGRKVTFHFKPDNGPAGNLTIRVKQ"
     gene            2121..2810
                     /locus_tag="PLJ64_00745"
     CDS             2121..2810
                     /locus_tag="PLJ64_00745"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNC92050.1"
                     /translation="MQIENFLFSVSDALRWPVLVAVLLALAWSIVEIGILLAEIWRRR
                     WRSINALENAVDQAEVDIVQGDQIGAISALSSVSWSRPMRETMTSIVTLRPSPDAENR
                     IAKRLADYDYNSLRRLERTRILVRMGPALGLMGTLIPLAPALAGLADGNVTELTDNLR
                     VAFGVTVAGLLTGALSFGVSLIRDRIYAQDFSDVEFAAANLAPDKHLAGHFVPSPVEH
                     GHSTQVSMGNP"
     gene            2810..3211
                     /locus_tag="PLJ64_00750"
     CDS             2810..3211
                     /locus_tag="PLJ64_00750"
                     /inference="COORDINATES: protein motif:HMM:NF021440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2149 domain-containing protein"
                     /protein_id="HNC92051.1"
                     /translation="MAMKVTAKARSREDRAGDPLDGLVNLFDIGIILSVGFLLAALSS
                     LNLTTEALSQNDTSPANTVPENSVVTQPDETTEQITIEPGQTVVGQGEPLGTVYQLDD
                     GRTILVKPSTGTTGATGATGTTGVTGSTLNP"
     gene            complement(3431..4942)
                     /locus_tag="PLJ64_00755"
     CDS             complement(3431..4942)
                     /locus_tag="PLJ64_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNC92052.1"
                     /translation="MEPTAATESIAVMNPATGEQVGSVPVDSAGDVAAAVARIRGNQP
                     AWEALGFKGRRRWLNELRDWLLDNSDRVADTLKSETGKVRAEATIETPYLADQINFYG
                     SKAEKFLGEQKVRPNTMLAATRKLRLQYRPYQVVGLISPWNFPLVLSLGDAIPALMAG
                     AAVVMKPSEITPLGLNEIVDAWRNEIGAPDVLHVVNGGAETATALVDHSDFVGFTGSD
                     VTGKKVMARAAETLTPVSLELGGKDPMVVLEGGNMDRAVNGATWGSMLNSGQMCISVE
                     RIYVEDGAYDEFVEKLTDSVGELKQGIDGETAACDVGAMTMLSQLEKVEKQVDDAVEA
                     GARVLTGGKRGEGPGQWFEPTVLVDCTPDMSVMEDETFGPVVTVTRVSDEEEAIRLAN
                     DSRYGLCASVFGPRGRAEKVARRIEAGAVNVNDIVFNMLAPGLPMGGWKESGIGYRNG
                     EYGIRKYCRSEAIVSARTLLRSEPLWYPYTPERHALVSKVTKFIGGRGLRRFR"
     gene            complement(5066..6268)
                     /locus_tag="PLJ64_00760"
     CDS             complement(5066..6268)
                     /locus_tag="PLJ64_00760"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNC92053.1"
                     /translation="MKLRAFLFAALAGFVLFLTAGDSAGAAPKLGYTRIATAREPTAL
                     VSPPGYPRMLFVTERAGFVRVIANGRKLKQPLLDLTSKVGSSNIERGLIGLAFAPDFR
                     TSGRFYVDYTAKNGNSIVAGYRTVPGQPTRLARGGGRLVIQIPRVNNKGNHNGGHLAF
                     RGNLLYIAVGDGFDPGDAPDHARNLNSLRGKILRIDPRPDQGTGKPYRIPAGNPFVGL
                     PGRDEIFAYGLRNPFTFSFFNSGGEAMMSIADVGQLRYEELNVLPFAEARGANFGWKG
                     FEGFEPYNCGESLCPNGADPVVATGLKWPQLVYSHDEGCAIVGGLKISDPKLTAVRGR
                     IIYGDFCSGRIRTALPHSPTIVDDRPIGFSLPARHGVTLLNGFGVDGLKRLYAFSHLG
                     GIYRLIQR"
     gene            complement(6301..7518)
                     /locus_tag="PLJ64_00765"
     CDS             complement(6301..7518)
                     /locus_tag="PLJ64_00765"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNC92054.1"
                     /translation="MGRLTCLTAAVFATLALSSASLACAEGDGGNPPERAATGKVSVG
                     GPDAAGASAGHGKLRATRVGFFNEPVEIRHAPGFPNLMFVVEQPGRIMVIRRGRKLAR
                     PFLDINRLVSNDGGERGLLSVAFPPDYRKSKRFYVYYVDNAGNIRVAEFRRRTAVRAR
                     RHSGRTVIRIAHPVNSNHNGGRLGFLGHDLYFGTGDGGGAGDEPGNAQNLNSLLGKLI
                     RIDPRRSGKRPYTVPASNPFVGRRGRDEIFAYGLRNPFRWSFDRTSSKRNVRIAIGDV
                     GQDAFEEVNYLNLSRARGGNFGWNRWEGYTPFNGGGAGTIKPSLVLPHPPNCSVIGGL
                     VVRDRRLPALRGRYVFTDFCSGKILSFKPHLGRAAGRPTGLNIGQVTSFGDSLGGALF
                     VTSLNGSVYRIRQ"
     gene            complement(7590..8285)
                     /locus_tag="PLJ64_00770"
     CDS             complement(7590..8285)
                     /locus_tag="PLJ64_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92055.1"
                     /translation="MPRSIVTLGLVLGALVGALLVSGCGGGDDDGQFAGQPTGDFPVK
                     VLDATFKPLQTVADTYDLKIAVENTGEKTIPGLNTVINLPGEGSTLAFAYGDKQQGLA
                     MNQRPVWVLEEGWPKLAGTTGRGGATTADRRTFEFGELKPDQTATMIWRVVAVRPGNY
                     RLEYQISAGLGPDTRAVDNSGHPPTGILPVRISDFARLTEINEKGQVVPVSRAEQARV
                     EAQQQSADTELAP"
     gene            8422..9228
                     /locus_tag="PLJ64_00775"
     CDS             8422..9228
                     /locus_tag="PLJ64_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC92056.1"
                     /translation="MVKATANKAIEPAKEFFTETGEMMILTGRTVLSAIKPPYPYGNE
                     FIGQFLFALQLCWFPMLISCVAFGFGAPGLQAANFLSLFGALDRLGGFFVLASIREFA
                     PFVTAVVVAGVAGTAITADLGARKIREELDALQVLGVDPIKNLVVPRFLALMLITGML
                     DIYALLFGIAGGIGAELLYSQPLGGFFASLFSNASTTELAASIIKCTLFGAIIAIVCC
                     YKGMTATGGAAGVGRAVNEAVVTALLGVFIFNYLFTQTMLATNPDIQTIK"
     gene            9228..10028
                     /locus_tag="PLJ64_00780"
     CDS             9228..10028
                     /locus_tag="PLJ64_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC92057.1"
                     /translation="MATSWLQVPREWLESMGEIVRFCGAVMGLVFSGRVFKYFGEVLN
                     QAGILILGSTTVIWGLVFVLGLQCGIEGAYFNRSVGAPAYAGVFAAWCDLREVIPYAF
                     GYMMSAKVGTGIVAELGAMRISDEIDALEVMGVPPVTFLAATRLLGAWITLPFMYIAA
                     IGVGFLASYIAVVEQIGDVSSGGYQLIFWMFQNPPDVLFSLIKAMTMSTAIVLVGCYY
                     GYTASGGPVGVGTATAKSMVLNIVLVHIIGMLGTLVFWGANPRAPIGG"
     gene            10033..11157
                     /locus_tag="PLJ64_00785"
     CDS             10033..11157
                     /locus_tag="PLJ64_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC92058.1"
                     /translation="MSETEETPDAQGSEETPINSPEVEAAVESTGDGEVAAEMESADE
                     AAAADDAVGEGEAELLAGEANEETTGPIDAAVTNEPEPSNGKGFVASDGAVLGRDFVI
                     PGVGPGDNDPYKWHTGPKQKTTDNAIEIVDVVKQFGRTRILNGLNLGLPDDQISMVLG
                     PSGTGKSVLIKHIVGLLYPDHGDVIVKGDSVPDLSDDDLFEMRKKFGLLFQDGALFGS
                     MNIYDNVAFPLRQHTDKGEEEIESIVTRRLREVGLGEAHFKMPNELSGGMRKRAGFAR
                     ALVLDPEIVMFDEPDSGLDPVRTALLCELIKEVHAESGGCYMVISHDLGTARRIADFI
                     AVLWKGRIVESGPKDELFESDNEFVHQFLQADVVGPLAMD"
     gene            11159..13615
                     /locus_tag="PLJ64_00790"
     CDS             11159..13615
                     /locus_tag="PLJ64_00790"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNC92059.1"
                     /translation="MSRFFGGVAAATARRPWPVLILAVLLGAISIWAAGGMGSQNVTD
                     AFFDKGSSAYRQTEQADRYFGTDPVVIMAKGPLIETLDTYNLNRLATLETCLAGRIKR
                     GRGQLFRTCKEISDLHPVHTLAGPATFLGRAVAGITEVYNRQIERLSSLPDTPAGQAE
                     RSRLIALAAQVISKYGLVEAPTLEDPTFVRRVVFSEGGVRAGPKPKLNYIFPSPDAAQ
                     IVIRLRSDLTGAERTRAIGLIKEAASDPSIVLRRSQLVVSGSPVVFNGLNDELPVRVL
                     ILAAVAVLLMSLALWFTFGSIWRLLPLALALGGMAVAAGLLRLLGGEFSLASLGAAPI
                     LIGLTVDYAVQLQARFDETPPLPPEQAAREAAGLGVPMIATACVATAAGFGTLIFSSL
                     PLVSQFGLLLGGGVLICFAFTFLAGFALLGLRGRRRPEPGRGRLIGRVRNLVKSVLAT
                     AIMAPGRVLALAVLLGAIGWAVSTQAEVRTEVGQLLPSRAPVVRDLLDVEKTTGASGE
                     IDLIVRAPDVTDPAVVVWTDEVRNTILNQSGYDTNDPSCEGAELCPGPAIPDFVDPTA
                     RGLTSADIRGVLKALPASDRKAMIAGGLTGKGDPTVAKIAFALRAGSVDGQQEVIDRM
                     QSAISDSRGGEGPPPGVSAEVTGLPVVVASSANDLADSRYLLVAAGILAIALVLLLVY
                     RSPRRVLVPLVPIIVAGGWSALIVAALDLSLNPLSTVLAVLVTAIATEFGVIISGRYF
                     QEREAGATLAVALRQTYGRTGLAVATSGLTAIAGFAALGSSDVAMLRDFGLIAVIDLG
                     VALAGVALVLPAMLVWLERR"
     gene            13612..14277
                     /locus_tag="PLJ64_00795"
     CDS             13612..14277
                     /locus_tag="PLJ64_00795"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNC92060.1"
                     /translation="MKEAGKKPFSYMTLVGVIFLVVVLVALFNMLKTTDSGTVGIGEV
                     GIGDRAAPFAVPLAMSGLDGDANIDPDEACSVEGADVLRICDYFERPLVMSFWFTKGA
                     SSCIDQQDVFDEVAAKYRRQAGFVSINVRDDRDRVRELIGEHGWKVPVGYDRDGAVSN
                     IYRVGGCPTFLFFRKGGVLEDAEIGENDAGQLGAQVRSLIDGESKATQQESASDPESG
                     PTG"
     gene            14303..14761
                     /locus_tag="PLJ64_00800"
     CDS             14303..14761
                     /locus_tag="PLJ64_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92061.1"
                     /translation="MESTTRQSGPAERRESRTRFGKWADRLEETADERPPAPWGSFPI
                     GAVSVFVGLVLAVIGLINANPVQLAIGVGLGMLGGLELAIREHFTGFRSHTTLLAGFV
                     FVVAVGATFYAAHWILWQSLLLGAALFGATFWLLRKKFEKASGGLSYKLR"
     gene            complement(14864..15202)
                     /locus_tag="PLJ64_00805"
     CDS             complement(14864..15202)
                     /locus_tag="PLJ64_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92062.1"
                     /translation="MEVAATADSKSIASRPMPQHLQALERANRVRLARAALKRSIASG
                     EVSVTKVITDCPWQTETMTLSELLRAQSRWGRTRTRKLLASVGLSENKRLDTLTERQR
                     MLLVSQLRPH"
     gene            15552..16877
                     /locus_tag="PLJ64_00810"
     CDS             15552..16877
                     /locus_tag="PLJ64_00810"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HNC92063.1"
                     /translation="MKLLLGLGALAIVAMVIVACGSSDDSSDDSGNVAGVSADTCGEM
                     TYGGEGSPDALIVSDLPLQGDSADRSKQMNDAVTQVLDTAGWKAGNTTIGFQACDDSL
                     ADTGLWDEQTCKDNATAYSDDKSVIGVVGTYNSGCAQAMIPILNQADGGSVAMVSPGN
                     TAICLTESADTCTDGTPDSLYPAGDRNYARVVPNDAAQGAGLVTYAAGNGIKRVAVLF
                     AANDDTSYGQAQTFINASGPGGVKVTEQLEWDPEAASYTDLMNKVKKSDPDGILLAGL
                     TEQNGGQLIKDKVAVFGPNDGSVALMAPDGFAQQSTIDEAGPASAGMIATVPGRAPEL
                     LSDSGKQFVKQLESKTGGQPVEIYAPYAGQAAQVLLDAIESAGNDRAGVARALYGIKV
                     TDGITGDFTITDTGDPDVSPITVNKAGKEFEPVEVIEPKANLIKAARGQ"
     gene            complement(17011..18264)
                     /locus_tag="PLJ64_00815"
     CDS             complement(17011..18264)
                     /locus_tag="PLJ64_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNC92064.1"
                     /translation="MSETTTAPIDLFEKIKNHERREQLEAANEGGLNPYFRLLTSQAG
                     PVVEMEGRETIMLGSNNYLGLTGDERVKNAAREALDQYGTGVTGSRLLNGTTPIHVEL
                     EKELAGWMNTEDAIVFSTGYQANVGCLQAILGPADTVICDSGDHASLLDGMKLSGAKL
                     RPFRHNQTDKLEKMLQRAQGDGGGILVVVDGVYSMEGDLPDLPRIVELSKHYGARIMV
                     DEAHGVGVLGERGAGATELFGLEDEVDLRMGTFSKSLASCGGFIAGNHDVIDYMRITS
                     RSFIFSASGVPAAVGAALEATKICRTEGAPLFARLLDNARYLRHGLEDLGLKVVQPGK
                     MPDGSVADTPVVPVMVGEDWTAVLLWKALFDAGVYCNVAIHPAVPPSGALLRTSLMAT
                     HEKEQLDRALGIIGEVIKDFPTLTG"
     gene            complement(18335..18781)
                     /locus_tag="PLJ64_00820"
     CDS             complement(18335..18781)
                     /locus_tag="PLJ64_00820"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HNC92065.1"
                     /translation="MTAGETLETRRELEGDAWEALSRLWFSRKPVLMELARELDLFPP
                     QIMVIQNLDRPKPMREVAEYLSCNSSNLTGITDRLEERGLVKRTLDPDDRRVRLLVLT
                     AKGRKVQGRVNAFLRQPPLSMEALDNEELETLTRLLAKLDPAGLPG"
     gene            18848..19888
                     /locus_tag="PLJ64_00825"
     CDS             18848..19888
                     /locus_tag="PLJ64_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC92066.1"
                     /translation="MPEREAGQGAPNTQSNGIEVEGLVRQFKNGPKAVDGISLTVEPG
                     EIYGFLGPNGAGKSTTVHMLTTLLPPTAGSARVAGLDVANQGAEVRKLIGAALQEAAL
                     DPNLTGREHMKLQTALHGLPREERVRRGDELIERVGLSSAADRRVGGYSGGMKRRLDL
                     ALALVHRPRVLFLDEPTTGLDPQSRASLWEEVTRLSTQDGVTVFLTTQYLEEADQLAN
                     RVGIIDAGKIAAEGTPSALKAEIGRPSVEVLPVDPERRGEAEQILLRFGTAVPAARDA
                     LSVRLNEGINELAAIVRSFDSAGIELASLGLNEPTLDDVFLAKTGRHLEGAGEADSAE
                     MRLPEVEAAGSS"
     gene            19885..20721
                     /locus_tag="PLJ64_00830"
     CDS             19885..20721
                     /locus_tag="PLJ64_00830"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC92067.1"
                     /translation="MSGAVATASGSLAGPDSPGPGHSTLPIQVSAMASRSVKRLIRQP
                     ALVIPSVIFPLFLLAVNAGGLNAATHIPGFPTDSYLTFAIGFTFIQAAIFSTMGAGQS
                     LAQDIENGFFSRLVLTPMRPTALMAGQLAGLVLLGQFQALLYLSVGLAAGAHFEAGLA
                     GYFVLAALVSIISIGFGGIGLIMAIRTGSGEAVQGMFPLMFVLLFFSSMTLPRNLIEQ
                     DWYRFLATINPVSYLIEAIRSLFIYGWDTEALALGFGVATVVAVVSIIFAAHSLQGRM
                     LK"
     gene            20718..21494
                     /locus_tag="PLJ64_00835"
     CDS             20718..21494
                     /locus_tag="PLJ64_00835"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC92068.1"
                     /translation="MIPDRKYFSAGWAVAWRYLHNLYTKPAVFVPGLIFPLFFLAAFA
                     GGLSAVGDTPKFDYYDFTAFQFCFILIQASALAGVFVGFSIAGDFEQGMGRRMMLATS
                     RRSSILLGYAIGAALRLIVVWVIITGVSLLAGMEVRGSGYDLVGMYSLGLLANVVALL
                     FSAGIAFRFRSLQAGPLMQMPVFIALFLAPVYVPQELLTGWIEPVAKVNPVTALLNGA
                     RSLMAGDPTGILLAYGIAFGMIALLALWALRGLRRAEVAG"
     gene            21579..22718
                     /locus_tag="PLJ64_00840"
     CDS             21579..22718
                     /locus_tag="PLJ64_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92069.1"
                     /translation="MSKLEIRRVQSKKDLKKFVKVPFLVHRDHPEWVPPLIMDRMDFL
                     NRDKNPYFDHAEVELWIAERDGEPVGRVSAQIDRNWDEYQGGNVGQFGFFETIDDQEV
                     ASALLDAGCEWLAGQGREKVYGPMDFTTNDEIGIQISGFDIQPSLLENCHQPYFQERV
                     EGAGFSKAMDLLMWHLEMGKLAKGLEFHPAIMESAQQSLDEHGITIRNMRKSDLANEM
                     GRFHEVYNEAWGDNWGFVPITREEVEFHAKTLKMVIDEDWAMIAETADGETVGAALTL
                     PDFNQVLAKMNGRIFPFGWYHFLTGRRKIDSVRILALGVKKAYQHTGVAAALYVKHIQ
                     TTDPNGVMKGEAGWILETNEPMNRALEGMGGEVTKKFRIYEKTLA"
     gene            22984..23295
                     /locus_tag="PLJ64_00845"
     CDS             22984..23295
                     /locus_tag="PLJ64_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92070.1"
                     /translation="MAEEVDGELISEEDVPAPQSGGEVRALPREYESGGALPVLKNEV
                     TTAAIAVAGGALAGAATVAAVRAVSGGSRRKARKLGRRARPANVVASRSFLVDVHVLG
                     R"
     gene            23306..24322
                     /locus_tag="PLJ64_00850"
     CDS             23306..24322
                     /locus_tag="PLJ64_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92071.1"
                     /translation="MIAERVVRPVAPLRMPRLVGGDLVFRLRGPWLERLLILSDEAMI
                     VRVREAARGEFVFAAEPVDPALLEGPGSPCLKEAGEPALEEGISRLRFSLGVDDDLAG
                     FRNAFREDPLLGRALNGRLQYRPMRRPVAWEALLWAITEQLIEYSRAARIQRQLIRRF
                     GVRLSPGVALGADDGRNRLPRSPRGRKQPSLMTVPTPAAIAGAAPAALEACGLSPRRS
                     IAMVKVAREVASGRVDPADPAGDKRLLAIPEIGPWTIACLALRGRGEPDALLAGDLSQ
                     VKLVGYLEGLGRLAEVEEVEEFYAPYAPWRGLAGEYIAMAYGSAVHGPGNRARIRQQL
                     RPAA"
     gene            complement(24405..25430)
                     /locus_tag="PLJ64_00855"
     CDS             complement(24405..25430)
                     /locus_tag="PLJ64_00855"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNC92072.1"
                     /translation="MRRFSLLPLAIFVALAVTACGTKSEEVNPVRDPLTVAMDWYPNP
                     DHAGFLVAEKQGYFEDAGLDVSLDSPTDPSLPIKLVAAGKADLALSYEPEVLLAREQG
                     LDVVSVATVIGRPLTSMIWLKKSKVKSVRDLKGKTVSTAGIPYQDAYLKTILSRAGLS
                     ENDVKKVGVGQGLLPSIVSGRAQATLGPFWNIEGIELKKEGLKPTVSPVDKLGVPTYS
                     ELVLVAKGSRIDEDPDPIRLFITALGRGTGFAVSHPNQATSTVLEANDALKPEVTAAQ
                     MKATLPLLGAPGGAKDAVPFGHQSTVKWQAFINWMVQQGVLANSQRAVDALTNELLPS
                     KRIDVDQ"
     gene            complement(25489..26247)
                     /locus_tag="PLJ64_00860"
     CDS             complement(25489..26247)
                     /locus_tag="PLJ64_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC92073.1"
                     /translation="MKARIWLPPLLLVLFLLGLWQLAASSGFLADVLGLEDFLVPAPS
                     EIADVLWQDRDLIFDNAGTTLVEIAAGFGLALISGLAVAVAFRGSDLVRRAGYPLVVA
                     SQTIPVIVIAPVLVVWFGYGIWPKLLIISLICFFPITVNTLDGLRRVDPEAVRMMRSL
                     DGSRMTIFRRVEVPTALPGVFTGARIAAAVAVIGAVFGEWAGSDKGLGHLILQDNAQL
                     MTARMFASVFVLSLIAISLYALIGLLERAVVRWR"
     gene            complement(26244..27017)
                     /locus_tag="PLJ64_00865"
     CDS             complement(26244..27017)
                     /locus_tag="PLJ64_00865"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC92074.1"
                     /translation="MTATVSARGLTHRFGPLDVLGGVDLEIEAGALLALAGPSGSGKS
                     TLLEITGGLREQSGGLVEVNGSADAAGRLRGCAWMPQRDCLLPWYSALDNASLALVNQ
                     GKSRREARKSAAERFEHFGLEGFETARPDELSGGMRQRVAFIRTLLSAKPVLLLDEPL
                     AALDAITRAELQEWLTPVLRESGATVLLVTHDVEEAIYLADRVTVLSPRPGRSIATID
                     GMRGSPGRRDEVVSSPAFNRKREELLGLLRSDQARGAAR"
     gene            27161..28099
                     /locus_tag="PLJ64_00870"
     CDS             27161..28099
                     /locus_tag="PLJ64_00870"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNC92075.1"
                     /translation="MDFSIAILFFGFGIGVLVGMTGMGGGALMTPLLILLFGVSPTTA
                     IGTDILYAAVTKTVGGWRHFKMKTVNMGLVFWLACGSVPAAVFGVQLVSWLQDRLGED
                     KLDSLVYAVLGGTLLMVGIITLGRALILARTISERDDFKIELRHKVAAVTIGATTGFV
                     IGVTSAGSGTVIAVLLIAVFRLTPKRVVGTDVFHAAIVLWAAGIAHFGHGNVDLGLVG
                     NILLGSVPGVVVGASLMNRVNQDGIRIALGLVLILSGIFTVQKGDPVVWPIAAVVAGM
                     GFALILMAPRWYQHFRPPEAIAAEQRAAGKQPPEAD"
     gene            28223..28678
                     /locus_tag="PLJ64_00875"
     CDS             28223..28678
                     /locus_tag="PLJ64_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNC92076.1"
                     /translation="MRVSAKADYAVRAVAELAASPDGPVKGERLADSQDIPLQFLEHI
                     LLDLKHSGIVRARRGAKGGYWLAKPAEDVSIADVIRAVEGPIAHVQSAPPESITYTGS
                     ARELQNVWIAVRASLRDVLESTSLADLVSGNLPDNVQRLADDKDAWEPR"
     gene            28808..30424
                     /locus_tag="PLJ64_00880"
     CDS             28808..30424
                     /locus_tag="PLJ64_00880"
                     /inference="COORDINATES: protein motif:HMM:NF017591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF839 domain-containing protein"
                     /protein_id="HNC92077.1"
                     /translation="MTEGLTRRDALRAGAGAAGGMALAGGLLGRAIDAMGAPAVIGDG
                     PYGPLGTPDANGLRLPAGFTSRKIARSGENIGPRPYQFHILPDGMGSFKTSDGGFILV
                     SNSEAPYLPGLAEIGAGAIRFNKDFEVVDAYPILKETWVNCAGGVTPWGTWLSCEEIE
                     TGKVFECDPKGRKPFDGSNWIERPALGVFKHEACCVDPAGKQVYLTEDLGDGCLYRFT
                     PDRYPDLSSGRLDVASVANDGTVTWHAVPDPQYAGSVPTRHQVEAATHFRRGEGMWFD
                     AGKVYFSTTQDDRVYAYHPASAHLEVLYDGVALGDEAPLHDTDNVTVSPISGDLFVCE
                     DPGGNAVNGLQVCIISAEGEAAAFCEAPASGHKDSELTGPVFDPTGSRLYFSSQRAKF
                     PGETVSAGIIYEISGPFRKLREDEKPVDHKKPLVKVRTLGMPSLAGLVKNGQAFQIDV
                     HDQSFPVDVEARLIAPLRRRQGRGTREVTLGTLKLTLHRPGAKKIRLKIKEGYRKRIR
                     QRRISGARLELIARDAAGNQRKVIKQVSFR"
     gene            30421..>30623
                     /locus_tag="PLJ64_00885"
     CDS             30421..>30623
                     /locus_tag="PLJ64_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92078.1"
                     /translation="MIPAARFGAAVLLLASLVAGCGSSTDQEGGGNAAAKSGQATTAR
                     ERAADRAAARKARAAARQKAALAA"
BASE COUNT         5011 a        10094 c        10274 g         5244 t
ORIGIN      
        1 gtgatcgtcc ggaccgcggc ggagatgcgg gcggtcgagc tggccaatcc gttcaagagc
       61 gccgcctaca accggatcgg cgtcctgttt ctcgatcacc gcctgccgcg ggagaacatc
      121 aggaaggcga agggccggaa gaacgaggag atcgaaatcg gcaaacggga gatctacgtc
      181 cactacccgg acgggatggg caggtcgaaa ctgaacctcc ccgccgccaa gtacggcaca
      241 acgagaaacc tgaacacggt cagcaagctg gccgacatgg ccggcggctg atcagtcccg
      301 gtccttgacc agggggcagg actggcagag ctggccgcct tcggtgcggt aggccaggca
      361 gcaggtgctc cggaggtggt actcgtcgcc ccagtcgtcc tcggctcttt cgatcgggat
      421 cgcgagccgg gggtccgggc aatcgggacc gacggtcagc ctggtcagtt cgagcgcaag
      481 tccgggacgg tcgaaagcct tgccggacca gaccagtgcc tgggccagcc ggtcggcggc
      541 cgcgtgccag agcgtcttgg ccggtctgag gccctgggcg tcaagccagt caacggtcgg
      601 ggccagcagg tcggcgaaac cgcggcggaa cgcttcggcg aggacatcga ggtccgacgg
      661 tccggccgct gtggcgagcg attgcgggtc ggtagtttcg aggccccggt cgctggccac
      721 cgccgtcgcc cggaagaggc cgccctcggc tgccaacagg atgttctccg gggtcagctc
      781 cggcaactgg ccggcagcga cgatgaacga gccgccgagg tcggcgatcg cccgggccag
      841 gctctcgagc agccactccg cccgcaggtg atcctcgggc gggccggcca tgccgctgat
      901 ccgggaaagc aggagcgcct ggacgcggcc gcttccatcc agctccgagg cggagaacca
      961 gccgtcaccc gggcccttca ggcgacggac gatgatcccg taaccgtccg cggacgcggt
     1021 gccgatccgg ctcagggtcc ggtcgaaccc gttgatcgca tgcggcccgg ccatgcggtc
     1081 actgtttcac acggatggtg agattcccgg cagggccgtt gtcgggtttg aagtggaagg
     1141 tgaccttccg gccccggagc cgccggctcg cccgccagta gaggtatcgc accgccggac
     1201 cggccttgag ctccccgatt tcatggcggc cacgccgcac ggtgacgaag tcgctggtgc
     1261 cgttgagggt ccggatcgag aaccggacca gaccgcccgg acgcacccgg gaacggttcg
     1321 cgcccacggc caggcgggtc tttggcctgg tctcatccgg accgatccgg acccgggtcg
     1381 aagccacctt caccgaactc ccgggtgcga ccgactcgat ccggctgcgg atcaggtagc
     1441 tgccgggtgc ggtcggattg acagcaccga tacccggggt ggggatgcct ttttcctgct
     1501 ggttgaatcg caggctgagg cccatcaggc ggaaaccgtg gtagttgccg ggaaccgtga
     1561 agctgatcag ggtcgagcca tcgtcgacgg tcttttcacc ggccgggccc aggccggtcc
     1621 cgtcctcgac cgtggcggtg atcggatggt tgctgagctg ccagtcgagg gtccaggtcc
     1681 gggccccgtc gatcgccggt ccgttcgagt agatcgcctt gttgtcgaag tcaccggcgt
     1741 cggtgaccat gtccagcgag ccgatgcgga gatcggcggc gggggccgga tcgaggcgga
     1801 aaccctcgac cgcgatctcg aaatccaggg gctggggaac cgcggccgac tgcctgaccg
     1861 ggatttcgaa aggcacgctg accgcgccgg gagatcgatc ggtgagcagg ggcgtacgca
     1921 ccccggcgtc gaaggcctgg gcgaagttcg gcaggatcag aacgcaggcg aaggcgatca
     1981 gtcccaggct tgtcttcagg gggcgaagca tcgggcgggt cagcgtatag acacattcgg
     2041 ggccggacag ttgcgcggcc tgacagcgtt gctccgctat cttgaccctt cggctgccct
     2101 aagccccggg cgcctcaagc atgcagatcg aaaacttcct cttctccgtt tccgacgctc
     2161 tccgctggcc agtcctggtc gcggtgctgc tggccctggc ctggtcgatc gtcgaaatcg
     2221 ggatcctcct ggccgaaatc tggcgccggc gctggcggtc gatcaacgcg ctcgagaatg
     2281 cggtcgacca ggccgaagtc gacatcgtgc agggcgatca gatcggggcg atttcggcac
     2341 tttcctcggt ttcgtggagc cggccgatgc gcgagacgat gacctcgatc gtcaccctcc
     2401 gtccctcccc cgacgcggag aaccggatcg ccaagcgact cgccgattac gactacaact
     2461 cactgcgccg gctcgaacgg acgaggatcc tggtccggat gggccccgca ctcggcctga
     2521 tgggcaccct gatcccgctg gctccggctc tggccggact ggcggacggc aacgtgaccg
     2581 agttgaccga caacctgcgg gtcgcgttcg gtgtgaccgt ggcgggcctg ctcaccggtg
     2641 cgctctcctt cggcgtctcg ctgatccgcg accggatcta cgcccaggac ttttcggatg
     2701 tcgagttcgc cgccgccaac ctcgcgccgg acaagcacct ggccgggcat ttcgtgccgt
     2761 caccggtcga gcacgggcac agcacccagg tctcgatggg gaatccctga tggccatgaa
     2821 ggtgacggcc aaggcgagga gccgcgagga ccgcgccggt gatccgctcg acggcctggt
     2881 caacctcttc gacatcggga tcatcctctc ggtcggtttc ctcctggccg cgctttcctc
     2941 gctcaacctg accaccgagg ccctctcgca gaacgacacc tcaccggcga acaccgtccc
     3001 cgagaactcg gtcgttaccc aaccggacga gaccaccgaa cagatcacga tcgagcccgg
     3061 ccagaccgtg gtcggccagg gcgagccgct cggcaccgtc taccagctcg acgacggcag
     3121 aacgatcctc gtcaagccct caaccggcac gaccggcgcg accggcgcga ccggaaccac
     3181 cggagtaacc ggctccacgc tcaaccccta gccggcaaac cgttttcgag aagtcgagcc
     3241 tgaacggggt ttcggggata tcgagaaggg agttttgcgc atataacgct cccaactccg
     3301 atttcgattc gacccatcag gtcgagatcg gagttgagtg cgctatgcgg caataagtcg
     3361 tatctcgata tccccggaac cgggtcggcg tagccgccgg ggcggcgtta ccagcggggt
     3421 ttcggaggga ctatctgaag cggcgaaggc cgcggccgcc gatgaacttg gtgactttgc
     3481 tgaccagggc gtggcgctcc ggggtgtacg ggtaccagag cggttccgag cggagcaggg
     3541 tgcgggcgga aacgatcgcc tcggaacggc agtacttgcg gatcccgtac tcgccgttgc
     3601 ggtagccgat ccccgactcc ttccagccgc ccatcggcag gcccggcgcc agcatgttga
     3661 agacgatgtc gttgacgttg accgcgccgg cttcgatccg ccgggccacc ttctcggccc
     3721 ggccgcgggg gccgaagacc gaggcgcaga gtccgtagcg gctgtcgttg gcgaggcgaa
     3781 tcgcctcttc ctcgtcgctc acccgggtca cggtgacgac gggaccgaaa gtctcgtcct
     3841 ccatcacgct catatcgggg gtgcagtcga ccagcacggt cggctcgaac cactgacccg
     3901 gaccttcgcc gcgcttgccg ccggtcagta cccgggctcc ggcctcgacc gcgtcgtcaa
     3961 cctgcttctc gaccttctca agctgggaca acatggtcat cgcgccgacg tcgcaggcgg
     4021 cggtctcgcc gtcgatcccc tgcttcagct cgccgacgct gtcggtcagc ttctcgacga
     4081 actcgtcgta agcgccgtcc tcgacgtaga tccgctcgac cgagatgcac atctggcccg
     4141 agttgagcat gctgccccag gtcgcaccgt tgacggcgcg gtccatgttg ccgccttcga
     4201 gaacaaccat cgggtccttg ccgccgagtt cgaggctgac cggagtcagg gtctcggccg
     4261 cgcgggccat caccttcttg ccggtcacgt cggagccggt gaagccgaca aagtcggagt
     4321 ggtcgaccag ggcggtcgcg gtttcggcgc caccattgac gacgtgaagc acgtcggggg
     4381 cgccgatctc gttgcgccag gcgtcgacga tctcgttcag gccgagcgga gtgatctcgg
     4441 aaggcttcat gaccacggca gcgccggcca tcagcgccgg gatcgcgtcc ccgagcgaga
     4501 gcacgagcgg gaagttccag gggctgatca gaccgaccac ctggtaaggg cggtactgga
     4561 gcctgagctt gcgggtcgcg gccagcatcg tgttcggcct gaccttctgc tcgccgagga
     4621 acttctcggc cttggacccg tagaagttga tctggtcggc caggtagggg gtctcgatcg
     4681 tcgcttcggc ccgcaccttt ccggtttcgg acttgagcgt gtcggcgacc cggtcggaat
     4741 tgtcgagcag ccagtcgcga agttcgttca gccagcggcg gcggcccttg aagccaagcg
     4801 cctcccaggc aggctggttg ccgcggatgc gggcgaccgc cgcggcgacg tcgccggccg
     4861 agtcgaccgg gaccgacccg acctgctcgc ccgtggcggg gttcatcact gcgattgact
     4921 cggttgctgc cgtcggttcc acctgctgct cccgtctctc gactctcttc gcctccgggc
     4981 cggtttgccc ttggggactg tcgttacagg ttagcggggg ccggaaggaa ttcaccgaat
     5041 cgatccgctt tgccccgggg cggggtcagc gctggatcag ccggtaaatg ccgccgaggt
     5101 gactgaaggc gtaaaggcgc ttgaggccgt cgacgccgaa gccgttcagc agcgtgactc
     5161 catgcctggc cggcagcgaa aagccgatcg gcctgtcatc gacgatggtc gggctgtgtg
     5221 gcagggccgt tcggatccgg ccgctgcaga agtcgccgta gatgatcctt cccctgaccg
     5281 ccgtcagttt cgggtcgctg atcttcaggc cgccgacgat cgcacagccc tcgtcgtggg
     5341 agtagacgag ctgaggccat ttcaggccgg tggcaaccac cgggtcggcg ccgttcgggc
     5401 aaagtgactc gccgcagttg tagggctcga agccctcgaa acccttccag ccgaaattgg
     5461 ccccccgcgc ttcggcgaag gggaggacgt tcagctcctc gtagcgaagc tggccgacgt
     5521 cggcgatcga catcatcgcc tcgccgccgg agttgaagaa gctgaaggtg aaggggttgc
     5581 gcagcccgta ggcgaagatc tcgtcgcgtc cgggaaggcc gacgaaggga ttgccggccg
     5641 ggatccggta cggctttccg gtcccctgat ccggccgggg gtcgatccgc aggatcttgc
     5701 cgcgcaggct gttcaggttc cgggcgtgat ccggggcgtc acctgggtcg aagccgtcac
     5761 cgaccgcgat gtagagaaga ttgccgcgaa aggcgaggtg gcctccgttg tggttgccct
     5821 tgttgttgac ccgcgggatc tggatcacaa gccggccgcc gccccgcgca agccgggtcg
     5881 gctggccggg aacggtccgg tacccggcca cgatcgagtt cccgttcttc gccgtgtaat
     5941 cgacgtagaa gcggccgctg gtccgaaaat ccggggcgaa agcgagtccg atcagacccc
     6001 tttcgatgtt gctcgaaccg accttcgaag tgaggtcgag caggggctgc ttcagcttcc
     6061 tgccgttggc gattacgcga acgaaaccgg cccgctcggt gacgaagagc atccgcgggt
     6121 agccgggcgg ggaaacgagg gcggtcggtt ccctggcggt cgcgatcctg gtgtaaccga
     6181 gtttcggtgc ggcgccggcc gaatcgcccg cggtcaggaa gagaacgaaa ccggccaggg
     6241 ccgcgaacag gaacgcccgg agtttcaatc gggccgggcc cgtcgctggc cgggtcggat
     6301 tcactgcctg atccggtaga cgctgccatt cagcgaagtg acgaaaagcg caccgccgag
     6361 gctgtcaccg aaagaggtga cctggccgat gttgaggccg gtcgggcggc cggccgcccg
     6421 gccgagatga ggtttgaaac tgaggatctt tccgctgcag aaatccgtga acacgtaccg
     6481 cccgcgcagg gccggcaggc ggcggtcacg aaccacgagg ccaccgatca ccgaacagtt
     6541 ggggggatgg gggaggacca gcgagggctt gatcgtcccg gccccgcctc cgttgaaggg
     6601 ggtgtaaccc tcccagcggt tccagccgaa attgccgccc ctggcccggc tcaggttgag
     6661 gtagttgacc tcctcgaatg cgtcctggcc gacgtcgccg atcgcgatcc ggacgttgcg
     6721 cttcgagctg gtccggtcga aggaccagcg gaagggattg cggaggccgt aggcgaagat
     6781 ctcgtcccgg ccgcgacgac cgacgaacgg gttgctggcg ggcaccgtgt agggacgctt
     6841 tccgctgcgg cgggggtcga tcctgatcag cttgccgagc agcgagttga ggttctgggc
     6901 gttgccgggc tcgtcacccg cgccgccacc gtcaccggtg ccgaagtaaa ggtcgtggcc
     6961 gaggaaaccg agccggccgc cgttgtggtt gctgttgacc ggatgggcga tccggatcac
     7021 cgttcggccg gagtgccggc gggctcggac cgcggtccgg cgccggaact cggcgacccg
     7081 gatgttgccg gcgttgtcta cgtaatagac gtaaaagcgt ttgctcttcc tgtaatcggg
     7141 cgggaaggca accgaaagca gacctcgctc gccgccgtca ttgctgacca gccggttgat
     7201 gtcgagaaag ggccgggcca gtttcctgcc gcgtcggatc accatgatcc ggcccggctg
     7261 ctcgaccacg aacatcaggt tcgggaaacc gggggcgtgc cggatttcaa ccggttcgtt
     7321 gaagaagccg acccgggttg cccgaagttt gccgtgcccg gccgaggcgc cggcggcatc
     7381 cggcccgccg accgaaacct tgccggtggc ggcgcgctcc ggcgggttac cgccgtcacc
     7441 ttcggcgcag gcaagcgagg ccgacgagag cgcgagagtg gcgaaaacgg ctgcggtgag
     7501 gcaagtaagt cttcccatga tcttcatccg tacccgaaaa ccgaccgggc aagccaagtt
     7561 gcgaacggcc gggggacgac tttctcgaat cagggagcga gttcggtgtc ggccgactgc
     7621 tgctgggctt cgaccctggc ctgctcggcg cggctgaccg gaacgacctg gcccttctcg
     7681 ttgatctcgg tgagacgggc gaaatcgctg atcctgaccg gcaggatccc ggtcggcgga
     7741 tggccgctgt tgtcgaccgc ccgcgtgtcc gggccgaggc cggccgagat ctggtactcg
     7801 agccggtaat tgccgggccg caccgcgact acccgccaga tcatggtggc ggtctgatcg
     7861 ggcttcagct ctccgaattc gaaggttcgc cggtcggcgg tggtggctcc gccccggccg
     7921 gtggtcccgg ccagcttcgg ccagccttcc tcgagcaccc agaccggacg ctggttcatc
     7981 gcgagaccct gctgcttgtc gccgtaggca aaggccaggg tcgaaccctc gccgggcagg
     8041 ttgatcacgg tgttgagacc ggggatcgtc ttctcgccgg tgttctcgac cgcgatcttc
     8101 aggtcgtagg tatcggcgac cgtctgcagg ggcttgaagg tcgcgtccag aaccttgacc
     8161 gggaagtcgc cggtcggctg gccggcaaac tggccgtcat cgtcgccacc gccgcagccg
     8221 gaaacgagca gggcgccgac aagcgcacca aggaccaatc cgagggtgac gatggatctg
     8281 ggcatggatc tgcggagtgc tcccctcaag aggggcgcaa cataccacgc gtgtggcgac
     8341 ggtcacacac gccgggggta tcgcatagac tgcgcgccac ggcgggtcac gtgttgtcca
     8401 gcggggcctg gcccgggaga gatggtcaaa gcaaccgcaa acaaggcgat cgagcccgcg
     8461 aaggaattct tcaccgaaac cggtgaaatg atgatcctga ccggacgcac ggtcctgtcc
     8521 gcgatcaagc ctccgtaccc ctacggcaac gagttcatcg gccagttcct cttcgccctt
     8581 cagctttgct ggttcccgat gctgatctcc tgtgtcgcct ttggcttcgg cgccccgggg
     8641 ctccaagcag ccaacttcct ttcgctcttc ggagctctcg accgcctcgg cggtttcttc
     8701 gttctggcct cgattcgcga gttcgcaccg ttcgtgaccg ccgtcgtggt cgccggtgtg
     8761 gccggcaccg cgatcaccgc cgacctcggt gcccgcaaga tccgcgagga actcgacgcc
     8821 ctccaggtgc tcggcgtcga tccgatcaag aacctcgtcg tgccccgctt cctcgccctg
     8881 atgctgatca ccggcatgct tgacatctac gccctgctct tcggcatcgc cggcggcatc
     8941 ggcgccgagc tgctctactc gcagccgctg ggcggcttct tcgcttcgct cttctcgaac
     9001 gcctcgacca ccgagctggc cgcctcgatc atcaagtgca ccctgttcgg cgcgatcatc
     9061 gcgatcgtct gctgctacaa gggaatgacc gcgaccggcg gcgcggccgg cgtcggccgg
     9121 gccgtcaacg aggcggttgt caccgccctg ctgggggtat tcatcttcaa ctacctgttc
     9181 acccagacca tgctggcgac caaccccgac atccagacga tcaagtaatg gctacgagct
     9241 ggctacaggt cccccgcgag tggctcgaat caatgggcga gatcgtccgt ttctgcggcg
     9301 cggtcatggg gctggtcttc tccggccggg tcttcaagta tttcggcgag gtcctcaacc
     9361 aggccggcat cctgatcctc ggatcgacca cggtcatctg gggcctggtc ttcgtgctcg
     9421 gcctgcaatg cggaatcgag ggcgcctact tcaaccgctc ggtcggcgca cccgcctacg
     9481 ccggcgtctt cgccgcctgg tgcgacctgc gtgaggtcat cccctacgcc ttcggctaca
     9541 tgatgtcggc caaggtcggc accggcatcg tggccgagct cggcgcgatg cggatctcgg
     9601 atgagatcga cgccctcgaa gtcatgggcg taccgccggt caccttcctc gccgccaccc
     9661 gcctgctcgg cgcatggatc accctccctt tcatgtacat cgccgcgatc ggcgtcggct
     9721 tcctcgcctc ctacatcgcg gtggtcgaac agatcggcga cgtttcgtcc ggtggctacc
     9781 agctgatctt ctggatgttc cagaacccgc ccgacgtctt attcagcctg atcaaggcaa
     9841 tgacgatgtc cacggccatc gttctggtcg gctgctatta cgggtacacg gcatcgggcg
     9901 gcccggtcgg ggtgggtacc gccaccgcga aatcaatggt tctgaacatc gtgctggtac
     9961 acatcatcgg tatgttggga acgctggtct tctggggtgc gaacccgaga gcaccgattg
    10021 gaggctgaga ggttgagcga aacagaagaa acacctgacg cgcagggttc ggaggaaact
    10081 ccgatcaact ctcccgaggt cgaagccgcg gtcgaaagca ccggtgatgg cgaagtggcc
    10141 gccgagatgg aatccgccga cgaggccgcc gctgccgatg acgcagtggg ggagggtgaa
    10201 gccgaactgc tcgccgggga agccaacgag gagaccaccg gtccgatcga tgccgcggtg
    10261 accaacgagc ccgagccctc caacggcaag ggtttcgtcg ccagcgacgg tgccgtgctc
    10321 ggtcgcgact tcgtgattcc cggtgtcggg cccggtgaca acgaccctta caagtggcac
    10381 acgggaccga agcaaaagac caccgacaat gcgatcgaga tcgtcgacgt ggtcaagcag
    10441 tttggccgga cccggatcct caacggcctc aacctgggcc tgccggacga tcagatctcg
    10501 atggtgctgg gcccctcggg caccggcaag agcgtgctga tcaagcacat cgtcgggctg
    10561 ctctaccccg accacggaga cgtgatcgtc aagggtgact cggttcccga cctcagtgac
    10621 gacgacctct tcgagatgcg caagaagttc ggcctgctct tccaggacgg ggcgctcttc
    10681 ggctcgatga acatctacga caacgtcgcc ttcccgctcc ggcagcacac cgacaagggt
    10741 gaggaagaaa tcgaatcgat cgttacccgc cggctgcgcg aggtcggtct cggtgaagct
    10801 cacttcaaga tgccgaacga gctttccggc ggcatgcgca agcgcgccgg atttgcgcga
    10861 gccctcgtgc tcgacccgga gatcgtcatg ttcgacgagc ccgactcggg tcttgacccg
    10921 gtgcggacgg cgctgctttg cgagctgatc aaggaagtcc atgccgaatc aggcggctgc
    10981 tacatggtga tcagccacga cctcggcacc gcgcgccgga tcgccgactt catcgcggtg
    11041 ctctggaagg gccggatcgt cgaaagcgga ccgaaggacg aactcttcga atcggacaac
    11101 gagttcgtcc accagttcct gcaggccgac gtggtcggcc cgctcgcgat ggactgagat
    11161 gtcgaggttt ttcggcggtg tcgccgcggc caccgcccgt cggccctggc cggtgctgat
    11221 ccttgccgta ctgctgggcg ccatttcgat ctgggcggcc ggcggaatgg gcagccagaa
    11281 cgtcaccgac gccttcttcg acaaggggtc gagcgcctac cgccagaccg agcaggccga
    11341 ccgctacttc ggcacggatc cggtcgtgat catggccaag gggcccctga tcgagacgct
    11401 ggacacctac aacctgaatc gcctggccac cctcgagacc tgtctggccg gaaggatcaa
    11461 gcggggcaga ggtcagctct tccgcacctg caaggaaatc tcggacctcc acccggtcca
    11521 caccctggcc ggtcctgcca ccttcctcgg ccgcgcggtg gccggcatca ccgaggtcta
    11581 caaccggcag atcgagcggc tgtccagcct gccggacacc ccggccgggc aggccgagcg
    11641 cagccgcctg atcgccctgg cggcccaggt catctcgaag tacgggctgg ttgaagcacc
    11701 caccctcgaa gacccgacct tcgttcgcag ggtcgtcttc agcgagggcg gggtccgggc
    11761 cgggccgaag ccgaagctga actacatctt cccttcaccc gacgcggccc agatcgtgat
    11821 ccggctgcga agcgacctga ccggggcgga gcggacccgg gcgatcggtc tgatcaagga
    11881 agcggcaagc gacccttcga tcgtgcttcg ccgctcgcag ctcgtggtct ccggctcgcc
    11941 ggtggtcttc aacggcctca acgacgagct gccggtcagg gtgctgatcc tggccgccgt
    12001 ggcggtgctc ctgatgagcc tggcgctctg gttcaccttc ggctcgatct ggcgccttct
    12061 gccgctcgcg ctcgccctcg gtggaatggc cgtcgcggcc ggtctgctca ggctcctcgg
    12121 cggcgagttc tccctcgcct cgctgggcgc ggccccgatc ctgatcgggt tgacggtcga
    12181 ttacgcggtg cagctccagg cccgcttcga cgagacaccg ccgctgccac cggagcaggc
    12241 cgcccgcgaa gcggccggcc tcggggtgcc gatgatcgcc accgcctgcg ttgcgacggc
    12301 ggccggcttc gggacgctga tcttctcctc cctgccgctg gtcagccagt tcggcctgct
    12361 tctcggcggc ggggtgctga tctgtttcgc cttcaccttc ctcgccggat ttgccctgct
    12421 ggggctgcgc ggccggcgcc gccccgaacc gggccggggc cggctcatcg gccgggtgcg
    12481 gaacctcgtc aagtcggttc tggcaaccgc gatcatggca ccgggcaggg tgctcgccct
    12541 ggccgtgctg ctcggcgcta tcggctgggc ggtcagcacc caggccgagg tccggaccga
    12601 ggtcggccag cttctgcctt cccgcgcccc ggtcgtccgg gatctgctcg acgtggaaaa
    12661 gacaaccgga gcctccggtg agatcgacct gatcgtccgg gcgcccgacg tgaccgatcc
    12721 ggcggtggtc gtctggaccg atgaggtcag gaacacaatc ctcaaccagt ccggttacga
    12781 cacgaacgac ccttcctgcg aaggggccga actctgtccc gggccggcga ttccggactt
    12841 cgtcgacccg accgcgcgcg ggctgacttc ggccgacatc cggggggtcc tcaaagcgct
    12901 cccggccagc gaccgcaagg cgatgattgc cggcggcctg accggcaaag gggatccgac
    12961 cgtggcgaag atcgccttcg cgctccgggc cggttcggtt gacgggcagc aggaagtgat
    13021 cgaccgcatg cagagcgcga tctccgacag tcgcgggggc gagggcccgc cgcccggcgt
    13081 gagcgccgaa gtgaccggac tcccggtcgt ggtggcctcg tcagccaacg acctggccga
    13141 ctcccgctat ctgctggttg ccgccgggat cctggccatc gcgctggtgc tgctcctggt
    13201 ctaccgctcg ccgaggcggg tcctggtgcc gctggttccg atcatcgtcg ccgggggatg
    13261 gtccgccctg atcgtcgccg ccctcgacct ttcgctcaac ccgctctcca ccgtgctggc
    13321 ggtgctggtc accgcgatcg ccaccgagtt cggggtgatc atctccggcc gctacttcca
    13381 ggagcgtgag gccggcgcga ccctggcggt tgcgctccgc cagacctatg gccggaccgg
    13441 gctggccgtc gcgacctccg gcctcacggc gatcgccgga ttcgcggcgc tcggatccag
    13501 cgacgtggcg atgcttcggg acttcggcct gatcgcggtg atcgatctcg gcgttgcgct
    13561 ggccggagtg gcgctggtcc tgccggcgat gctggtctgg ctggagcgcc gttgaaggaa
    13621 gccgggaaga agcccttcag ctacatgacc ctggtcgggg tcatcttcct ggtggtcgtc
    13681 ctggtcgcgc tcttcaacat gttgaaaacc accgactcgg gcacggtcgg gatcggggag
    13741 gtggggatcg gtgaccgggc ggctcccttc gccgtgccgc tggcgatgtc cggactcgac
    13801 ggggacgcca acatcgaccc ggacgaggcc tgctcggttg aaggtgccga cgtgctgagg
    13861 atctgcgact acttcgagcg gccgctggtc atgagcttct ggttcaccaa gggcgcttcg
    13921 agctgcattg accagcagga cgtattcgac gaggtcgccg cgaagtaccg ccggcaggcg
    13981 ggctttgttt cgatcaacgt ccgcgacgac cgtgaccggg tccgggaact gatcggcgag
    14041 cacggctgga aggttccggt cgggtacgac cgggatggcg cggtttccaa catctaccgg
    14101 gtcggcggct gcccgacctt tctcttcttc cgcaagggcg gggtgctcga agacgccgag
    14161 atcggcgaga acgacgccgg acagctgggg gcgcaggtac gctccctgat cgatggcgag
    14221 tcgaaggcaa cgcagcagga aagcgcgtcg gacccggaat ccggtcccac cggctgaggc
    14281 ggcccgggcc gacgccgggt ccgtggaaag cacgacgcgg cagtccgggc ctgccgagcg
    14341 ccgcgagtcg cgcacgaggt tcggcaagtg ggccgaccgg ctggaggaaa ccgcggacga
    14401 gcggccgccc gcaccctggg gcagctttcc gatcggcgcg gtctcggtct tcgtcgggct
    14461 cgtgctcgca gtgatcggcc tgatcaacgc caacccggtt cagcttgcga tcggagtcgg
    14521 cctcggcatg ctgggcgggc tcgaactggc gatccgcgag cacttcacgg ggttccggtc
    14581 acatacgacg ctgctggcgg gtttcgtctt cgtggtcgcg gtcggggcga ccttctacgc
    14641 ggcccactgg atcctctggc agtcgctcct gctcggcgcg gcactgttcg gagccacctt
    14701 ctggctgctc aggaagaagt tcgaaaaggc ctccggaggc ctcagctaca agctccggtg
    14761 actcaactgc agtcgggcgg ggtccaaccc gccctcccag tagaactcgc cccttccccc
    14821 gaaaaaaaga atggagggaa ggggctcagt ctggtggtgc ctctcagtgg gggcggagct
    14881 ggctgacgag cagcatgcgc tggcgctcgg tcagggtgtc aaggcgcttg ttctcgctca
    14941 ggccgaccga ggccaggagc tttctcgtcc gggtccggcc ccagcgggac tgggcgcgga
    15001 gcagttccga gagggtcatc gtctcggtct gccagggaca gtcggtgatc actttggtaa
    15061 cggaaacttc accgctggcg attgatcgct tcagggctgc gcgagcaagt cgaacccgat
    15121 tggcccgttc gagagcctgc agatgctggg gcatcggacg gctggcaatc gacttagaat
    15181 cggcggtggc ggcaacttcc acgggcggtc ctcccttcgg gggattggtc ttgttgactg
    15241 gatggcatgg ggcgacaggc cagggatcct ctttcccggg tggcgcttct cccccgcctg
    15301 gtgccgggct ttccgtccga ctcaggcgat aaaggcaaca tagacaagtt ggccggaccg
    15361 ggcaatggtc agggccgcga actttcgcta agagcaggtt tttctttttc gccgaattcc
    15421 cttgtcgggg acctgcccgg aatgtatggt cgaggcacgg tctgaccgaa cactgttcgg
    15481 tcggcgcaag gttcaagtgg cctccagtcc gacccggcca cctacagacg ggagagagag
    15541 tgtccaagca cgtgaagctt ctactcggtc tcggcgccct ggccatcgtg gccatggtca
    15601 tcgttgcctg cggcagtagc gacgactcat cggatgattc cggaaacgtt gccggcgtga
    15661 gcgccgacac ctgcggtgag atgacctacg gcggcgaggg ttcacccgat gccctgatcg
    15721 tctccgatct gccgctccag ggtgattctg cggatcgctc gaagcagatg aatgacgcgg
    15781 tcacccaggt gctcgacacc gcgggatgga aggccgggaa cacgacgatc ggcttccagg
    15841 catgtgacga ctcactcgcg gacaccggac tgtgggatga gcagacctgc aaggacaacg
    15901 ccaccgccta cagcgatgac aagagcgtga tcggtgtggt cggcacttac aactcgggct
    15961 gcgcccaggc gatgatcccg atcctcaatc aggccgatgg cggctcggtg gcgatggtct
    16021 cgcccggcaa cacggcgatc tgcctgaccg aatcggcaga cacctgcacc gacggcaccc
    16081 ccgactccct ctacccggcc ggcgatcgca actacgcgcg ggtcgttccc aacgatgccg
    16141 cccagggggc cggtctcgtt acctacgcgg ccgggaatgg catcaagcgg gtcgcggtcc
    16201 tgtttgccgc caatgacgac acctcgtacg gacaggcgca gaccttcatc aacgcctccg
    16261 gtccgggcgg cgtcaaggtc accgagcagc tcgagtggga tccggaagca gcgagctaca
    16321 ccgatctcat gaacaaggtg aagaagagcg acccggacgg catcctgctc gccggcctga
    16381 ccgagcagaa cggtggtcag ctgatcaagg acaaggtcgc ggtcttcggt ccgaatgacg
    16441 gcagcgtggc gctgatggcg ccggacgggt tcgcccagca gtcgaccatc gacgaggccg
    16501 gcccggcctc cgcgggcatg atcgccacgg ttcccggccg cgcccccgag ctgctgtcgg
    16561 attccggcaa gcagttcgtg aagcaactgg aatcaaagac cggcgggcag ccggtggaga
    16621 tctacgcccc ctacgccggt caggcagccc aggtcctgct cgatgcgatc gagtcggccg
    16681 gaaacgaccg ggccggggtt gccagggcgc tctatggcat caaggtgacc gatggcatca
    16741 cgggcgactt cacgatcacc gacaccgggg atccggacgt cagcccgatc acggtcaaca
    16801 aggccggcaa ggagttcgag ccggtcgagg tgatcgagcc gaaggcgaac ctgatcaagg
    16861 ccgcccgggg tcagtaggaa ccagcagaag aactgatccc ggtgtgccgg actcctcgac
    16921 ccgaaacgtc gtcgcccggc acaccgggat cagtccaatc aagactgtcg gtcctccggg
    16981 ccgcagcccc acgcgagcgg ggcgccctgg ctaaccggtg agggtgggga agtccttgat
    17041 gacttcgccg atgatcccga gggccctgtc gagctgctcc ttctcgtggg tggccatcag
    17101 gctggttcgc agcagggcgc cgctgggcgg gaccgccggg tggatcgcca cgttgcagta
    17161 gacgccggcg tcaaagaggg ccttccagag caggaccgcg gtccagtcct cgccgaccat
    17221 gacaggcacc accggggtgt cggcgaccga gccgtccggc atcttgcccg gctggaccac
    17281 cttgaggccg aggtcctcga gaccatggcg caggtagcgg gcgttgtcga gcaggcgggc
    17341 gaacagcggc gcgccctcgg tccggcagat cttcgtcgcc tcgagcgcgg caccgaccgc
    17401 ggcgggcacg ccggaagcgc tgaagatgaa ggagcgggag gtgatccgca tgtagtcgat
    17461 gacgtcgtgg ttgccggcga tgaagccgcc gcaggaggcg aggctcttcg agaaggtgcc
    17521 catccgcagg tcgacctcgt cttcgagacc gaagagttcg gtcgcgccgg caccgcgctc
    17581 gcccagcacg ccgaccccgt gggcctcgtc gaccatgatc cgggcgccgt aatgcttcga
    17641 tagttcgacg atccggggca ggtcgggcag gtcgccttcc atcgagtaga cgccgtcgac
    17701 cacgaccagg atgccgccgc cgtcgccctg ggcccgctgg agcatctttt ccagcttgtc
    17761 ggtctggttg tggcggaagg ggcggagctt ggccccggaa agcttcatgc cgtcaagcag
    17821 cgaggcgtgg tcgccggagt cgcagatcac cgtgtcggcc gggccgagga tcgcctgcag
    17881 gcagccgacg ttggcctggt atccggtcga gaagacgatc gcgtcctcgg tgttcatcca
    17941 gccggccagt tccttttcga gttcgacgtg gatcggcgtg gtgccgttca ggaggcgcga
    18001 gccggtgacc ccggtgccgt actggtcgag ggcctcgcgg gccgcgttct tgacccgttc
    18061 gtcgccggtc agcccgaggt agttgttgga accgagcatg atcgtttccc ggccttccat
    18121 ttcaaccacc gggccggcct gggaggtgag caggcggaag taggggttga ggccgccttc
    18181 attggcggct tcgagctgct ctcggcgctc gtggttcttg atcttctcga agagatcgat
    18241 cggtgcggtc gtggtctcgg acatgcccgg attctctcag aaccggggcc ggggttgacg
    18301 gaattgaccg gggcggcgaa gcaagtggcc gcggtcagcc gggaaggccg gcagggtcca
    18361 gtttcgccag cagccgggtc agggtttcga gttcctcgtt gtcgagggct tccatcgaga
    18421 gtggcggctg gcgaagaaag gcgttgaccc ggccctggac cttgcggccc ttggcggtga
    18481 ggaccagcag gcggacccgg cggtcgtcgg gatcgagggt gcgcttgacc aggccgcgtt
    18541 cctccagacg gtcggtgatc ccggtcaggt tcgagctgtt gcaggagagg tactcggcga
    18601 cctcacgcat cggcttgggc cggtcgaggt tctggatgac catgatctgg ggcgggaaca
    18661 gatccagctc gcgggccagt tccatcagga ccggtttgcg tgaaaaccag agccggctga
    18721 gggcctccca cgcgtccccc tcgagctcgc gtcgtgtttc gagggtctcg cctgcggtca
    18781 tttgcaccag tataccattc aaatgccgga tagttcaagt cttgaacatt tgctatacat
    18841 aacgatcatg ccggagcgag aagcgggcca gggtgcccca aatacccagt cgaacgggat
    18901 cgaggtcgaa gggctggttc gccagttcaa gaacggaccg aaagcggttg acgggatcag
    18961 cctgaccgtc gaaccagggg agatctacgg cttcctcggc ccgaacgggg cgggcaagtc
    19021 aaccaccgtc cacatgctga ccaccctgct gccgccaacc gccgggtcgg cccgcgtggc
    19081 ggggctcgac gtggccaacc agggggcgga ggtgcgcaag ctgatcggtg ccgccctgca
    19141 ggaagctgcc ctcgacccga acctgaccgg ccgcgaacac atgaagctcc agaccgcgct
    19201 gcacgggctc cccagggagg aacgggtccg gcgcggtgac gagctgatcg aaagggtggg
    19261 gctttccagc gcggccgatc gccgggtcgg cggctactcg ggagggatga agcggcgcct
    19321 ggacctggct ttggccctcg ttcaccggcc ccgggtgctc tttctcgacg agccgaccac
    19381 cggccttgac ccgcagagcc gtgcctcgct ctgggaggaa gtcacccgcc tttccaccca
    19441 ggacggggtg acggtctttc tgaccaccca gtacctcgag gaggcagatc agctggccaa
    19501 ccgggtcggg atcatcgacg ccgggaagat cgccgccgaa ggaactccct ccgcgctcaa
    19561 ggccgagatc ggccggccga gcgtcgaggt gctgccggtc gatcccgaac gccgcgggga
    19621 ggccgagcag atcctgctgc gcttcggcac cgcggttccg gccgcccggg atgcgctcag
    19681 cgtgcgtctc aacgaaggca tcaatgagct ggcggcgatc gtcaggtcat tcgattcggc
    19741 cggaatcgag ctggccagcc tcggcctgaa cgagccgacc ctcgatgacg tcttcctggc
    19801 caagaccggc cgccacctgg aaggggccgg cgaggccgac agcgccgaga tgcggttgcc
    19861 cgaggtcgaa gcggcaggtt cctcgtgagc ggagccgttg caaccgcgag cgggagcctg
    19921 gccgggccgg acagccccgg ccccgggcat tcgacgctgc cgatccaggt ctcggcgatg
    19981 gcgagccggt cggtcaagcg gctgatccgc cagcccgccc tggtaattcc cagcgtgatc
    20041 ttccccctct tcctgctcgc cgtgaacgcg ggcgggctca acgcggcgac ccacataccg
    20101 ggcttcccga ccgattccta cctgaccttc gcgatcggct tcaccttcat ccaggcggcg
    20161 atcttctcga ccatgggggc gggccagagc ctcgcccagg acatcgagaa cggcttcttc
    20221 agccgcctgg tgctgacccc gatgcggccc accgcgctca tggccggcca gctcgccggt
    20281 ctggtcctgc tcgggcagtt ccaggcgctg ctctacctct cggtcggtct cgccgccggg
    20341 gcgcatttcg aggccggtct ggccggctac ttcgtgctgg ccgccctggt ctcgatcatc
    20401 tcgatcggct tcggcgggat cggcctgatc atggcgatcc gcaccggctc cggcgaggcg
    20461 gtccagggca tgttcccgct catgttcgtg ctgctcttct tcagctcgat gaccctgccg
    20521 cgcaacctga tcgagcagga ctggtaccgc ttcctcgcca cgatcaaccc ggtcagctac
    20581 ctgatcgagg cgatccgcag cctgttcatc tacggctggg acaccgaagc cctggcgctc
    20641 ggctttggcg tggcgaccgt ggtcgcggtg gtttcgatca tcttcgcggc ccactcgctt
    20701 caggggagga tgctcaagtg atccccgatc gcaagtactt ctccgcgggc tgggcggtcg
    20761 cctggcgcta cctgcacaac ctctacacca agccggccgt gttcgtgccc gggctgatct
    20821 tcccgctgtt cttcctggca gcctttgcgg gcggactctc cgcggtcggc gacacgccga
    20881 aattcgacta ttacgacttc accgccttcc agttctgctt catcctgatc caggcttcgg
    20941 ccctggccgg ggtcttcgtc ggtttctcga tcgccggtga cttcgagcag gggatgggca
    21001 gacggatgat gctggccaca tcgcggcgct cctcgatcct gctcggatac gcgatcggcg
    21061 ccgccctgag actgatcgtg gtctgggtga tcatcaccgg cgtctccctg ctggccggga
    21121 tggaggttcg cggcagcggc tacgacctgg tcggcatgta ctcgctcggc ttgctcgcca
    21181 acgtggtcgc cctgctcttc tcggccggga tcgccttccg tttccgttcg ctccaggccg
    21241 gaccgctgat gcagatgccg gtcttcatcg cgctcttcct cgcgccggtc tacgtgccgc
    21301 aggaacttct gaccggctgg atcgagccgg tcgcgaaggt caatccggtg accgccctgc
    21361 tcaacggcgc ccgcagcctg atggccggcg acccgaccgg gattctgctc gcctacggga
    21421 tcgccttcgg aatgatcgcc ctgctggcgc tctgggctct gcgcggactg cgccgggccg
    21481 aggttgccgg ctgagtccgt cgcgcggcgg caccgacctc ccggccgcca ggggcggcgc
    21541 aaccggatcc cggggtccgg tccgctagtt tcccggcaat gtcgaagctt gagattcgcc
    21601 gggttcagag caagaaggac ctgaagaagt tcgtcaaggt ccccttcctc gtccaccgcg
    21661 accaccccga atgggtgccg ccgctgatca tggaccggat ggacttcctc aaccgtgaca
    21721 agaacccgta tttcgatcac gccgaggtcg agctgtggat cgccgagcgc gacggtgagc
    21781 cggtcgggcg ggtcagcgcc cagatcgatc gcaactggga tgaataccag ggcgggaacg
    21841 tcgggcagtt cggcttcttc gagacgatcg acgaccagga ggtcgcttcg gccctgcttg
    21901 acgcgggctg cgagtggctg gccggccagg gtcgggagaa ggtctacggg ccgatggact
    21961 tcaccaccaa cgacgagatc ggcatccaga tttccggctt cgatatccag ccgtcgctgc
    22021 tcgaaaactg ccatcagccc tatttccagg aaagggtcga gggtgcgggt ttcagcaagg
    22081 cgatggacct gctgatgtgg catctcgaaa tgggcaagct cgccaaggga ctcgagttcc
    22141 acccggcgat catggaatcc gcgcagcaga gtctcgacga gcacgggatc acgatccgca
    22201 acatgcgcaa gtccgacctc gccaacgaga tgggacgctt ccacgaggtc tacaacgagg
    22261 cctggggcga caactggggc ttcgtcccga tcacccgcga ggaagtcgag tttcacgcca
    22321 agacgctcaa gatggtgatc gacgaggact gggcaatgat cgccgaaacc gccgacgggg
    22381 agaccgtcgg cgccgctctc accctgcccg acttcaacca ggtcctggcg aagatgaacg
    22441 gccggatctt cccgttcggc tggtaccact tcctgaccgg tcgcaggaag atcgactcgg
    22501 tccgcatcct cgccctcggg gtcaagaagg cctatcagca caccggcgtc gccgctgccc
    22561 tctacgtgaa acacatccag accaccgatc ccaacggcgt gatgaagggc gaggcgggct
    22621 ggatcctcga aacgaacgag ccgatgaacc gggccctcga aggcatgggc ggcgaagtga
    22681 ccaagaagtt caggatctac gagaagacct tggcctagcc gcagacgccg cggagcgggt
    22741 agccctccca aagatggcgc taccgtcgcg ctcgtcccca cctgctcgct aatcggtgca
    22801 gtaatcggga agccactttg ggagggccac ccgccccacg gctacacgag tctcgcctca
    22861 ggttcgcgag atcccagttt gcgccacata ccggcacaaa gtgggcactc ggcggggttt
    22921 gaatcgtcgg tatttgcgga cttatgtcaa gtgaccgccg atggcgactg tagattggcg
    22981 gtcgtggctg aagaggttga tggtgaactg atttccgagg aggacgttcc ggcgccgcag
    23041 agcggtggcg aagttcgcgc acttcctcgc gaatacgagt ctggcggggc tcttccagtg
    23101 ttgaagaacg aggtaacgac cgcggccatc gccgtggccg gcggtgcact tgccggtgcc
    23161 gctaccgtgg cggccgtgcg ggccgtcagc ggcggctcca ggcgcaaggc ccgcaagctg
    23221 ggccgccggg cgcgcccggc caacgtggtc gccagtcgct cttttctggt tgacgtccac
    23281 gtcctcggac gataggggtg gtgcggtgat cgccgagcgc gtggtcaggc ccgttgcacc
    23341 gctacgcatg ccccgcctgg ttggcggtga cctggtcttc aggctgcgtg gcccctggct
    23401 tgagcggctg ctgatccttt ccgatgaagc gatgatcgtc cgggtccgcg aggcagcccg
    23461 tggcgagttc gtcttcgcgg ccgaaccggt cgatccggct ctgctcgaag gtccgggctc
    23521 gccctgcctg aaggaggcgg gggaaccggc gctcgaggag gggatctcca ggttgcgctt
    23581 cagcctcgga gttgacgacg acctggccgg gttccgcaac gcatttcgcg aggatccgct
    23641 gctcggccgg gccctgaacg gtcgtctcca gtaccggccg atgcggcggc cggtcgcctg
    23701 ggaggccctg ctttgggcaa tcaccgagca actgatcgag tacagccggg cggcccggat
    23761 ccagcggcag ctgatccgcc gcttcggggt ccgcctctcg cccggtgtgg cgctcggcgc
    23821 cgatgacggc cgaaaccggc ttccccgctc gcctcgcggc cggaaacagc cgagcctcat
    23881 gaccgtgccc acccccgcgg cgatcgccgg tgccgctccg gccgctctcg aggcctgcgg
    23941 gctcagtcca cggcgttcga tcgccatggt caaggttgcc cgcgaggtcg cctcgggccg
    24001 ggtcgacccg gcggacccag ccggggacaa gcgcctgctg gcgatcccgg agatcggccc
    24061 ctggacgatc gcctgcctgg ccctgcgtgg ccgcggtgag cccgacgcgc tgctggccgg
    24121 cgacctcagc caggtcaaac tggtcggtta cctcgaaggc ctgggccggc tggccgaggt
    24181 cgaagaggtt gaagagttct atgccccgta cgcaccctgg cggggcctgg ccggcgagta
    24241 catcgccatg gcctacggtt cggcggttca cggcccgggc aaccgggccc ggatccgcca
    24301 gcagctgcga cccgccgcat aacggagtcc gttcatcaag tcgttgatgt cctgaacgga
    24361 cgcacgggtt tcattggcat tccggcgcag accggaatgc gtacttactg atccacgtct
    24421 atccgcttgc tgggcagaag ctcgttggtc agggcgtcga ccgcgcgctg cgagttggcc
    24481 agcactccct gctggaccat ccagttgatg aatgcctgcc acttcacggt gctctggtga
    24541 ccgaacggaa ccgcgtcctt cgcgcctccg ggcgcgccca gcagcggcag ggtcgccttc
    24601 atctgggcgg ccgtgacctc gggcttcagg gcgtcattgg cttcgagcac ggtgctggtt
    24661 gcctggttgg gatgggagac cgcaaaaccg gttccccggc cgagtgccgt gatgaagagg
    24721 cggatcgggt ccgggtcttc gtcgatccgg ctgcccttgg cgaccaggac cagctcgcta
    24781 taggtcggga cgccgagctt gtcgaccggg ctcacggtcg gcttgaggcc ttccttcttc
    24841 agttcgattc cctcgatgtt ccagaagggg ccgagcgtcg cctgggcccg gccgctgacg
    24901 atcgacggca gaaggccctg gccgaccccg accttcttca cgtcgttctc gctcagtccc
    24961 gcccgcgaga ggatcgtctt caggtaggcg tcctggtagg gaatcccggc ggttgaaaca
    25021 gtcttgccct tgaggtccct gacactcttc accttcgact tcttcagcca gatcatcgag
    25081 gtgagcggcc ggccgatcac cgtcgcaact gaaaccacgt cgagaccctg ctcgcgggcc
    25141 agcagcacct ccggctcgta ggaaagcgcg aggtcggcct tgccggcggc gacgagcttg
    25201 atcgggagcg agggatcggt cggggaatcg agcgaaacgt cgagcccggc atcctcgaag
    25261 tagccctgct tctcggcgac caggaagccg gcgtgatcgg ggttggggta ccagtccatc
    25321 gccacggtca ggggatcccg gaccgggttc acttcctccg acttggtgcc gcaggcggtg
    25381 accgcgaggg caacgaagat tgcgaggggg agcagtgaga atcgtctcaa gtcatctccg
    25441 gggacgtgaa caggatggga aggagcctat agaggttatt gccgccagct accgccaacg
    25501 cacgacagcc cgttccagca ggccgatcag ggcgtaaagg ctgatcgcga tcagggagag
    25561 cacgaagacc gaggcgaaca tgcgcgcggt catcagctgg gcgttgtcct ggaggatcag
    25621 gtgccccagc cccttgtcgg atccggccca ttcgccgaag accgcaccga tcacggcgac
    25681 cgcggcggcg atcctcgccc cggtgaatac cccgggcagg gcggtcggca cctcgacccg
    25741 ccggaagatg gtcatccggg agccgtccag tgagcgcatc atccgcaccg cttccgggtc
    25801 gacccggcgg aggccgtcca gcgtgttgac ggtgatcggg aagaagcaga tcagactgat
    25861 gatgagcagc ttcggccaga tcccgtagcc gaaccagacc accagaaccg gggcaatcac
    25921 gataaccggg atggtctggg aggcgaccac cagcgggtag cccgcccgcc ggaccaggtc
    25981 cgaaccgcga aaggcgaccg ccaccgcgag gccggagatc agggccaggc cgaagccggc
    26041 ggcgatctcg accagggtcg tgcccgcgtt gtcgaagatc aggtcgcggt cctgccagag
    26101 cacatcggcg atctccgatg gtgcgggcac gaggaaatcc tcgagaccga gcacgtcggc
    26161 gaggaagccg ctcgaagcgg ccagctgcca gaggccgagc aggaagagga ccagcagaag
    26221 cggaggcagc cagatccttg ccttcaacgg gctgcccccc gggcctggtc gctgcgaagc
    26281 agccccagca gctcctcgcg cttccggttg aaggccggcg agctgaccac ctcgtcgcgg
    26341 cggcccgggc tgcctctcat cccgtcgatc gtggcgatgc tccgcccggg ccggggggag
    26401 aggacggtga cccggtcggc caggtagatc gcctcctcga cgtcgtgggt caccagcagc
    26461 accgttgccc cgctttcccg gagcaccgga gtcagccatt cctgcagctc ggcccgggtg
    26521 atcgcgtcga gcgcggcgag cggctcgtcg agcagcagta ccggtttggc ggaaaggagg
    26581 gtgcggatga aggcgacgcg ctgcctcatg ccaccggaaa gctcgtccgg gcgggcggtc
    26641 tcgaaaccct ccaggccgaa gtgctcgaac cgttccgccg ccgactttct cgcctcccgc
    26701 cgggatttcc cctggttgac cagggccagg ctggcgttgt ccagggccga ataccagggc
    26761 agcaggcagt cgcgctgcgg catccaggcg cagcctcgca gccgccccgc ggcgtcggcg
    26821 ctgccgttca cttcgaccag tccgccggac tgctcccgca ggcccccggt gatttcaagc
    26881 agggtcgact tgccggatcc cgagggcccg gccagggcga gcaacgcgcc ggcttcgatc
    26941 tcgaggtcga ctccccccag gacgtccagc ggtccgaatc tgtgggtcag cccgcgggca
    27001 gaaacggttg ctgtcatcgc cgggattgta ggtccgctgc ccaaaccact aggatgccga
    27061 cattcccgca ttccttacta aacaagtaag gagagtcggg agcgctccta gcccggcgcg
    27121 ccagcaccgt cacagcatcg atcctgcctc agcgaaccca gtggactttt caatagcgat
    27181 cctttttttc ggcttcggca tcggagtcct ggtcggcatg accgggatgg gtggcggtgc
    27241 cctgatgact ccgctcctga tcctgctgtt cggggtttcg ccgaccaccg cgatcggcac
    27301 cgacatcctc tacgccgcgg tcaccaagac ggtcggcggc tggcgccact tcaagatgaa
    27361 gacggtcaac atgggcctcg tcttctggct cgcctgcggg tccgtgccgg ccgcggtctt
    27421 tggagttcag cttgtttcct ggcttcagga ccggctcggt gaagacaagc tcgattcgct
    27481 ggtctatgcc gtactgggcg ggacgctgct catggtcggc atcatcacgc tcggccgggc
    27541 gctgatcctc gcccgcacta tcagcgagcg ggatgacttc aagatcgaac tccggcacaa
    27601 ggtcgccgcc gtcacgatcg gggccaccac cggattcgtg atcggcgtca cctcggccgg
    27661 ctccggaacg gtgatcgcgg tgctgttgat cgccgtcttc cgactgaccc ccaagcgcgt
    27721 ggtcggaacc gatgtctttc acgcggcaat cgtgctctgg gccgcaggga ttgcccattt
    27781 cggccatggc aacgtggacc tcggcctggt cggcaacatt ctgctcgggt cggtccccgg
    27841 cgtcgtggtg ggcgcgtcac tgatgaaccg ggtcaaccag gacgggatcc ggatcgccct
    27901 cggcctggtg ctgattctct ccggcatctt caccgttcag aagggcgacc ccgtggtctg
    27961 gccgatcgcc gcggtggtgg ccggcatggg cttcgcgctg atcctgatgg caccgcggtg
    28021 gtaccagcat ttccggcccc cggaagcgat cgccgcggaa cagcgggcag ccgggaaaca
    28081 gcccccggaa gccgattagc actgccttcc gcgcctcggg aagcgaaatt cccgattctg
    28141 ctgaccgatt tgatcgcgaa ataccgtgac cgggcccggc agcctgcgga cccgcgccgg
    28201 ttcgggctga tttactaggc ccatgcgcgt ctccgccaag gccgattacg ccgtcagggc
    28261 agttgccgaa ctcgccgcgt ctccggacgg gccggtcaag ggtgaacgac tcgccgattc
    28321 ccaggacatc ccgcttcagt ttctcgagca catcctgctc gacctgaagc actcggggat
    28381 cgtccgcgcc cgtcgcggcg ccaagggtgg ctactggctg gccaagccgg ccgaagacgt
    28441 ttcgatcgcc gacgtgatcc gcgcggtcga aggtccgatc gcccacgtcc agtcggctcc
    28501 gccggagtcg atcacctaca ccggcagcgc cagggaactc cagaatgtct ggattgccgt
    28561 ccgcgcaagc ctccgtgacg tgctggaatc aaccagcctc gccgacctcg tctccgggaa
    28621 cctcccggac aacgttcagc ggctggccga tgacaaggac gcctgggaac cgaggtaggt
    28681 tccccgggcg gcgcgaggga ccactgcccg cccatgactt gttttgcccc tggccttgcc
    28741 ctgaccggta gcctgctttc agggagagtt tttctacgat ttccagggag tgggtatgtc
    28801 agaggaagtg actgaaggac tgactcgaag agacgccttg cgggccgggg ctggcgccgc
    28861 cgggggaatg gctctggccg gcggcctgct gggccgggca atcgacgcga tgggcgctcc
    28921 ggcggtgatc ggcgacggcc cctacggtcc gctcgggacc ccggatgcca acggcctccg
    28981 gctccccgcc ggcttcactt cccgaaagat cgcccgctcc ggtgagaaca tcgggccccg
    29041 cccgtaccag tttcacatcc tgcccgacgg catgggctcc ttcaagacca gcgacggcgg
    29101 cttcatcctc gtttcgaaca gcgaggcccc ctacctgccg ggcctggccg aaatcggagc
    29161 cggagcgatc cgcttcaaca aggatttcga agtggtcgac gcctacccga tcctcaagga
    29221 aacctgggtc aactgcgcgg gcggcgtgac cccgtgggga acctggcttt cctgcgagga
    29281 gatcgagacc ggcaaggttt tcgaatgtga ccccaaaggc cggaaaccgt tcgacggcag
    29341 caactggatc gaacgcccgg cgctcggcgt gttcaagcat gaggcctgct gcgtcgatcc
    29401 ggccggcaag caggtttacc tgaccgagga tctcggggac ggttgtcttt accgcttcac
    29461 tcccgaccgg tacccggacc tttcctccgg ccggctcgac gtcgcttcgg tcgctaacga
    29521 cggaaccgtc acctggcatg cggtcccgga cccccagtac gccggctcgg ttcccacacg
    29581 tcatcaggtc gaggcagcga ctcatttccg gcggggcgaa gggatgtggt ttgacgccgg
    29641 gaaggtctat ttctcgacca cccaggacga ccgcgtttat gcctatcacc cggcttcggc
    29701 tcatctcgag gtgctctatg acggggtcgc cctcggcgac gaagctcccc tccatgacac
    29761 cgacaacgtg accgtctcac cgatctccgg cgaccttttc gtctgcgagg acccgggcgg
    29821 caatgccgtc aacggcctgc aggtctgcat catctcggcc gagggcgagg cggccgcctt
    29881 ctgcgaggcg ccggccagcg gccacaagga ttcggagttg accgggccgg tattcgaccc
    29941 gaccggaagc cggctctact tcagttcaca acgggcgaag ttccccggtg aaaccgtgtc
    30001 cgccggaatc atctacgaga tcagcggccc cttccggaaa ctgcgggagg acgaaaagcc
    30061 ggtcgatcac aagaagccgc tggtcaaggt gcggacgctg gggatgccaa gcctcgccgg
    30121 gctcgtcaag aacggccagg ccttccagat cgatgttcac gaccagagct tcccggtcga
    30181 tgtcgaggcc cggctcatcg cgcccttgcg ccgccggcag ggccgcggca cacgcgaagt
    30241 gacgctcgga accctgaagc tcacccttca ccgtccgggc gccaaaaaga ttcgcctgaa
    30301 gatcaaggag ggctaccgca agaggatcag gcagcggcgg atctccgggg ccagactgga
    30361 gctgatcgcc agagacgccg ctggcaatca gcgaaaagtg atcaagcagg tcagtttccg
    30421 ttgatcccgg cggcccgctt cggggcggcc gttttgctcc tcgccagcct tgtcgcgggc
    30481 tgtggttcat ccacggacca ggaagggggc ggaaacgccg ccgccaaatc cggccaagcg
    30541 accacggccc gggaacgggc tgccgatcgt gcggcagccc gcaaagccag ggcggccgcc
    30601 cgccagaagg ccgcgcttgc cgc
//
LOCUS       DAOQKN010000006        29152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_640_length_29152_cov_6.503626, whole genome shotgun sequence.
ACCESSION   DAOQKN010000006 DAOQKN010000000
VERSION     DAOQKN010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 29152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29152
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_640_length_29152_cov_6.503626"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(269..2224)
                     /locus_tag="PLJ64_00890"
     CDS             complement(269..2224)
                     /locus_tag="PLJ64_00890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HNC92079.1"
                     /translation="MVQTEAGLLVLKRTSAWSVAIALAVVGLLVFSSLAQAANPLPDS
                     QRADLPKPGNYLPVNGVWAERLTGYMDARDGMDLKYSVLLPPGDGPFPAIMNYSGYDP
                     GSIGGTAYQAGNTTMDPELDAKLLKAGYAVVGVNMRGTGCSDGNFSLFANEWGTDGYD
                     AVEWTADQPWSNGKVGMANWSYAGLSQILTAVNNPPHLKAIAPGMAVTDQWRDVASPG
                     GVTNWLFPYGWGLFIQQRWNAAKTSAEVEGDTKCIDNIAAHNAGFNAVSPYREGVERP
                     HVDNKPGRTNHVWRQVHKIKAAVISMVAWQDEATGPRAGYYQNRLDPKRSYLVGTNGP
                     HDIYMADRWQAMLIPFFDRYVKGKKNGFEKKPHVRLWFDTKTPDGSSTKSGNLRKSTP
                     GTVLTQKSLPIKVKPLRLSLRSSGSLTTNKPRKPEPASSYDFPTVSPNVNPDYAEPGD
                     TDWQEDPADPNGTVAFTTAPLQRTLTFQGPASVDLWVSATTPDADIQATITEVRPDGK
                     EVYVNRGWLRLSERALDKKLSTPTLPFLKRTKGAVQMLKDSKPVYGRVEIMQFAHIFR
                     KGTSIRVLLDTPSTTGGYGFEGPRDDTKIKVFHGPNRNSKLVLGLMRVGGIKTPKPAC
                     DTVLRQPCRENTFSIPTGKGPDGSTAR"
     gene            complement(2405..4123)
                     /locus_tag="PLJ64_00895"
     CDS             complement(2405..4123)
                     /locus_tag="PLJ64_00895"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNC92080.1"
                     /translation="MSEITGGELFAKCLASEGIEHVFGLPSPEIDPLFAQLEKYGIRL
                     MPVRHESAGVHMAEGLYKSTGKASCVIGNPGPGSANLLPGIFTALHEGVPVLAITSQH
                     RMGVSYPSPPSTFQGQDQLDAFRPAVKWGGPIFEWDRIPEVLRLAFREMWNGRPGPVH
                     VDVPVTVMYDLGEESEQPILAPGAYRPSQPQASAAQIGAAADLLESASRPIVVAGAGV
                     DRAGANAQAAELAELLGCPLIPTQAGRASVRSDHPNYVFGFGEGGIKARLEADVILVA
                     GSRIGNLDLPYDKYWGDPENQSLIQIDTDPSHLGVTRPLAIGMVADAADALGGITAEL
                     KRRDKTPSAEATAALAEAREADAAWQAVQASTADDWEGEGIHPARAIEVIGETFGDQA
                     IYTVDGGLTSVWAYAGLPPTPPRSYHSILELGMLGTGIPSAIGSKIGCPDRDVVCVTG
                     DGAAGFNIMEMQSAARDSIPVTVIVFAEGSWTMEEPNELALFNRTFGTEQGETRWDLV
                     AEGLGCHGEFVDQLGDLGEALVRCRDSGRPSVVCLKTNRDANRVIPPAMAARWMEVQA
                     GPIPGV"
     gene            complement(4291..5676)
                     /locus_tag="PLJ64_00900"
     CDS             complement(4291..5676)
                     /locus_tag="PLJ64_00900"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNC92081.1"
                     /translation="MNGNLARELANLLSPEQVVTIGPELRAALQDGTGNRGIKGEAEA
                     LLLPADAEEVAEVMAWCYGHGVPLTVRGGGTGLAGGAVPDGGVVLSLERLARVRSIDP
                     ELWRMEVEAGVRTATVHRIALENGLMFPPDPGASEQSTIGGNIATNAGGPHAFRYGVT
                     GAWVTGIEAVVPPGEVLSSGGPLRKNVAGLDLTDLMIGSEGTLGIVTSAWLRLVPAPE
                     ERGVVVGFYPDTASGCEAIGMIMGSGIQPTALEFLDRGALESSLGSFPSEAPEQAGFA
                     VIAEADGSAGEVNQAVGDLEEAMAGGALQPPVRITDHVAIRNFWAWRDGVSIAVTAQR
                     GGKISEDVVVPVESLAAAVDETVEIGRRHDLPACSWGHAGDGNLHSSFLVDLADDDQM
                     KRGGAAAAEIFEMAIRLGGSISGEHGIGSLKTGHVEAALGPVAVRLQAEIKRVFDPKL
                     LLNPGKKIPLP"
     gene            5813..7567
                     /locus_tag="PLJ64_00905"
     CDS             5813..7567
                     /locus_tag="PLJ64_00905"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNC92082.1"
                     /translation="MGLTGGEFVAEYLIAEGVPYVFGIPGHGDMAAFDAFKDRADRIT
                     MIGPRHEQAAAHMADAYYRASGKPLATMTSIGPGSMNVATGLGTAFVDSIPLISFTGG
                     PQTYMLGRGVLQELERQQDNVFPHIIQPMVKRNWDVQHVDLLADVLPRAFNNMLSGRP
                     GPVHIELPMDVQAEVTELPVPDPAERRATSGAVRPDPDAIDKAAAKLLAAKRPVLLAG
                     GGCLEAKAAAEVKKVAEYLDAAVVVTMMGKGVFPEDHPLDAEHTGSNGTLVGNYMTKN
                     ADVILAVGTRFAEQTSSSFMDGMSFSIPPTEIIHLDIDPTEIGKNYPTAVGAVCDAKA
                     GLNDLFDALVDHNGTEGISRPAYREEIAKLRADWKEMLTGRWTGKLSLSQVLHKSREV
                     LPREAIAIASAGHPQIQAFQEFLSYEPRTWLSPGGYSTMGYTLPAAIGAKLAKPDVPV
                     VGFAGDGDLQQTIQELAVAAETGAAVIIACLNNTGWISIRDFQRGMFGEDRGFETEFR
                     SRKGDQLMPVDFCAIAEAFNCGSEKVTETGDIGPAFERAMASEGPCLIEFELSSEPAD
                     SEGVNIGHWDLPKPAYLP"
     gene            7591..8937
                     /locus_tag="PLJ64_00910"
     CDS             7591..8937
                     /locus_tag="PLJ64_00910"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNC92083.1"
                     /translation="MNHDTPERDPNGAAQQLKNESDDHVIKCMGDVDMGLVKGEGVRV
                     WDADGNEYLDAISAEWVLNLGFRHPEIIEAMEAQFGKIDYVSPVFEDEARTAYAKKLA
                     ELAPGALSKVLYGLSGASAVEGAMHLAMRTTGGTDFVCLDAAFHGRTFATIGLTYVNP
                     GMVEGANKGLDRYLPRQMRVPNYNCYRCPLNLEPGTCDMACAEAVDWALDKGHLNGPA
                     GVIVEPYQANGGMVPAPDGYLERVHEIAKSHEVPLIVDEIQGALCRCGPMYASQRAGI
                     EPEMIILGKALGGGLPLSATITTPEYSELLPWEYGFTQAGNPMACAAGLAMVEIMVRD
                     DLPANSERIGTMMIEELEKIQEDSKLIGDVRGQGLMIGVELVRDRETREPAFEETDQL
                     MAACLEHGLMIGKTGPVFGANGNVAKFKPAVNVTEEDAMEMIDLFKRALGDVEATL"
     gene            8934..9719
                     /locus_tag="PLJ64_00915"
     CDS             8934..9719
                     /locus_tag="PLJ64_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNC92084.1"
                     /translation="MTREISFIAALDYEERPAAEVLESLLEAGYDSVEWTMAHAEELL
                     APASALACQMDLVTGGEKAAEETIRGIEAAAAAGIGVVNVLTGPNLWEEGAVQRDDEA
                     AWKVALEALEWIVARGEAAGVAIGFEPCWGTLAHDAQTAQRVLDSVGVSVTFDPSHFV
                     MTGDPIPELIERWGERIVHFHLKDAFGRPGIDGEDFIFCMLGEGKVPWPETFAALDRV
                     GYEGPLSVEFEAYAYYEQVLGSDPAAAARLARGQVAALLGEGS"
     gene            9716..10717
                     /locus_tag="PLJ64_00920"
     CDS             9716..10717
                     /locus_tag="PLJ64_00920"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNC92085.1"
                     /translation="MKVALCGLGEIGQIHLRAIRESGHELVGVLELNRRLAVESVGDE
                     VPIHTEVMEMVELTEPDVVDICLPHHLHVPIAVAALEAGADVLLEKPMAIDLAGCDEI
                     IAAAEAFDHRVGISHNQIHFAPHRRLRELIDAGRLGQIRAIYERLWMGGKYGGWREDS
                     SQVGGGLLMDAGVHRVYMAEYLGGPVSTVSAVMDKPRSEDSFVLTLEFESGATGVIQG
                     GYHGPEGVFDDRIEIQGSAGMAEALGCEAFFEGDLSGDLRLRARLDGAWVDDPVTGDW
                     DESVIASVRACLDDLAAGREPESGLAAGRSAVAVIEAAYRSAETGNSIRVSDLDGRQ"
     gene            10717..11457
                     /locus_tag="PLJ64_00925"
     CDS             10717..11457
                     /locus_tag="PLJ64_00925"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNC92086.1"
                     /translation="MKSIDIGNEEKGSLREIKAESSLTARVRESIREAIIDGSLEPGS
                     LHSVKSLADIFKVSRTPVREALIDLAGADMVEFERNRGVRILETSVHDLEEILVLRIL
                     LEVPATYRAAEHIDADGLSALRQELDAMVEAAENGDEPTMMQHDRRFHELINTASGNS
                     RLTEYVDSLRDLILTRGVSTVDNTRSLTEIVDEHEGILTAIASGDGEAAAAAMKEHLV
                     NTASLLLQQEGGRSSSLSLGWERLVAGG"
     gene            11454..12362
                     /locus_tag="PLJ64_00930"
     CDS             11454..12362
                     /locus_tag="PLJ64_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC92087.1"
                     /translation="MSKLVKLAVASWRRQKLRTAMTVGSLALSVGAVVFVYSLSVAFE
                     SSGSQAIESAIGKADIWVVPAKGVEVNRKDESIDAIGSLPSGIEAEVRSLPAVTAVTA
                     VTGSGAGTLQVTTSNPKLTADAMKSKGLTVTSDPARSTATGDAPALAYLITASEDRFG
                     VYTFSQQFEAVQVNEVASSVLGVVGRVTLALGFLAVLTSLLISIDERRREFGILAAVG
                     ITDDVLYLFLVESGLLIAMGLVVGVIFGGGMFALLLPSIFSLGTVGKAVALVSVYFPI
                     MLILGALIPAWRLLQKSPLELLRSSP"
     gene            12359..12913
                     /locus_tag="PLJ64_00935"
     CDS             12359..12913
                     /locus_tag="PLJ64_00935"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNC92088.1"
                     /translation="MIKPLKYSLRMITRARGSSLLRSGAVAVAAALTFFVLAQSSTVR
                     NQATDLAVGGIGVDTIERATLSLTVIAVIVGALQVGVLMTRVVLQRMQEIGVLKAAGV
                     SSRDIFLVFLFESLIYGFTGGVIGCLVGLLIALLGPMPGSEEILRAFVITVALSTVVA
                     TLAGLAPARRAVQAPAVEAINHAW"
     gene            12943..13695
                     /locus_tag="PLJ64_00940"
     CDS             12943..13695
                     /locus_tag="PLJ64_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC92089.1"
                     /translation="MNEHEQVPGGTGPVVETEDLWKLFKLGDEVVKALKGVSLKIHPG
                     ELVVIMGPSGSGKSTMLHLMGGLERPTKGEIYLEGQPLSGLTESQLSTMRHQAVGFIF
                     QSYNLIPLLTATENVELPLVFDDVDSGQVRRDCVELLERVGLGHRLDHTPSRMSGGEQ
                     QRVAIARALIGKPRLLLADEPTANLDHKNGDAIVKLLTTLNHEMGMTTVISTHDRSVA
                     EHASRVIEMRDGKVVSDSGSGDGAPADRLATA"
     gene            13703..15088
                     /locus_tag="PLJ64_00945"
     CDS             13703..15088
                     /locus_tag="PLJ64_00945"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNC92090.1"
                     /translation="MTAISSVNPFDPSDQVAGCEGASAAEIDSVLAAGVAAQPGWAAD
                     PMLRLRSLSTLADEIEVRRQKFIDLMVREVGKPVAEAAGEVDRAIAILGFYSQVPLDP
                     VGEVVPGSVPGARVLVERHPLGLVLAICPWNFPLAIPLWKSAPALAYGNAVVIKPASP
                     AIATAALLAECAAGALPEGVLSVLPMSGARTSEVLDDERIAAVTFTGSTGVGISVAER
                     MASRAAPAQAEMGGQNAAIVLEDADLDAAADAIISGAMGFAGQKCTATRRVVVVSQVR
                     EQLEAKLVERVRGLSVGDPEQSGVTVGPLISESAVEEFEAARSAALEAGATELAVAEA
                     PEGEGYFVSPALLRQDDPAATVNQEETFGPLLTLIEVADEDAAVEAANATRFGLVGAV
                     HGRDLGRAGRVAGRMTTGLRRVNAPTPGVDFYAPFGGEGQSSFGPREQGRAAREFFTS
                     SSTTTILPSAT"
     gene            complement(15286..15654)
                     /locus_tag="PLJ64_00950"
     CDS             complement(15286..15654)
                     /locus_tag="PLJ64_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92091.1"
                     /translation="MLGLSALSIALPASFAPETFYTDYPFYTALVKLLPPYNEHLITD
                     VGGLYLGFGVMFIWATIKPSRELVLPLCWGWIAAQILHFAFHVSHLTGFTTTQAVSQQ
                     IGLALYLLVAIVPIALLRRQ"
     gene            complement(15765..16949)
                     /locus_tag="PLJ64_00955"
     CDS             complement(15765..16949)
                     /locus_tag="PLJ64_00955"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNC92092.1"
                     /translation="MKTRGSSRLILFAAVLLGLVAAASPASAIRPDSQAFLSPVDFQI
                     VNRPTPVRGADDRRHMVYEIQAVNQSGLEVEIRKVAPRAQGNRLGLPFTGSRLEERMR
                     INSGATGTTLGPGGSAMIFIDASYSLGRKRPKAISHAISLAWADPETPSEPVGHTFLG
                     VSTRVARQKPVVLSAPLRGSKWVAVNGCCTLNAHRGATLAIDGTVRVPERFAIDFVQL
                     NDQNRLYSGLKVDLFSYGYYRTPVYSAAPGRVVGVRRNLPEQIPGSLPAGATIQMAGG
                     NHVVVDIGHGRYAFYAHLATNSIRVRRGQKVKAGQVIGLLGNTGNTDGPHLHFHVMNS
                     PSPLQSSGLPFVFRNFRGQGVLGNVAGIQNGEVASINHNRLRGVYRRVLPMENQVVRF
                     GR"
     gene            complement(16959..17987)
                     /locus_tag="PLJ64_00960"
     CDS             complement(16959..17987)
                     /locus_tag="PLJ64_00960"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNC92093.1"
                     /translation="MATLRSRIALALIKKQLPKIVAADPVAELGDGLHVAVVGSGSPL
                     PDDKRGNPCTLVIAGEKVFVVDAGEGASETINRMGIDPGRIEALLLTHFHSDHIGGIP
                     SVNLQRWVADGTDTPMPVLGPPGAERVVKGFNDAYRLDRSYRTAHHGEDYVDPAIGPM
                     AVEEFPVPGEGESIVVLEDDGLVVSAFEVDHSPVAPVVGFRFDYQGRSAVISADTVYS
                     GNLVKAAKNTDLLIHDALSIELLMMVAEAQTEAGNPARGKILADVHDYHATAPQAADA
                     ARQAGARALALTHVVPPLPLKGLEDIYLADAPDRFDGPLWIAADGDLYSLPAAGGLKR
                     SNLISRRR"
     gene            complement(18021..18920)
                     /locus_tag="PLJ64_00965"
     CDS             complement(18021..18920)
                     /locus_tag="PLJ64_00965"
                     /inference="COORDINATES: protein motif:HMM:NF002964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HNC92094.1"
                     /translation="MTGFRTEHLETLVALVEEGTFEAAARRLSVTPSAISQRIKAMER
                     STGQVLVQRSNPVEPTTAGDIALRYGRQMRLLEDEAARALALEESGGGAVTVPLAINA
                     DSLGTWFMEALARVDSPGVVFEVFREDQEHTTSLLRAGKVMAAVTSNAEAVQGCRSEL
                     LGTMTYHPVCSPGFLEGQLGGEATPANLGKATVVDFDRKDQTQNRFFREFTGKPLRAP
                     RHYIPTTADYARAILLGLGWGLLPDRQCLEEIKRGDLVEFAPDQAIEMPLYWQRWTLE
                     SPLLEVLTEAVKGVAAREMHPRD"
     gene            18993..19619
                     /locus_tag="PLJ64_00970"
     CDS             18993..19619
                     /locus_tag="PLJ64_00970"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE/ArgO family amino acid transporter"
                     /protein_id="HNC92095.1"
                     /translation="MTASLLQMMLLGFAASLTLIVAIGAQNAYVLRLGVEGRGRILVT
                     VILICAFSDAVLIAAGVAGIGAVLEAAPAAIDAVRILGAVFLISYGVIAARRALNPQA
                     MTAGESPADASMKAIVLTTLAFTWLNPHVYLDTVMLLGSIANQQQGVDRWWWALGAVA
                     ASFTWFFLLGYGARLLGPLFARPGAWRVLDALIAIIMVGLGAGLIIGG"
     gene            19763..20674
                     /locus_tag="PLJ64_00975"
     CDS             19763..20674
                     /locus_tag="PLJ64_00975"
                     /inference="COORDINATES: protein motif:HMM:NF039218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH hydrolase domain-containing protein"
                     /protein_id="HNC92096.1"
                     /translation="MVALTVAALVVAGCGGDGSSNKTEPAASQAATSQTLNNQEGESA
                     DGFVPSFSEVKQDKGPVFGDGCLIHGDEPESGECAYGDTESSRNVVVFGDSHALQWTP
                     ALIGIASQRGWRLTALLHANCTAAQVSVDENCDRWRENSLKRISEEKPGLVIVASNTG
                     PNMTVVSDGTKLDREAAGSSLEAGMARTFEDLKASGAEVTLMQDMAMSKDYLPSECVA
                     GHQDDPGQCTFEMDRPPELAYDLKAARQVGGVQVIDPLPKICPGYSCYPVHGRYLKFR
                     DRFHITATYSRLLAPWLDSRLQNPWEE"
     gene            20682..21536
                     /locus_tag="PLJ64_00980"
     CDS             20682..21536
                     /locus_tag="PLJ64_00980"
                     /inference="COORDINATES: protein motif:HMM:NF039218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH hydrolase domain-containing protein"
                     /protein_id="HNC92097.1"
                     /translation="MKHRGSSLLAGLLAIACLATVVMAGPATARFIPGLNRVRADKGE
                     VYRKGCLMGHNRVRSGPCRFGDRDSKKKVVLLGDSHAMQWGPGLIRLARKKQWQVIAL
                     TRASCPAALVHIDYYCDTWRRNSLRRIRLMRPGLVLVASAANSDAYSVFTGGGYLSRA
                     ASEQYLVDGMIRTLRRLRRWSKKTVVMQDQAVTPFSVTACLRINSPGNCGFRASRPRA
                     WSYDFKAARRVKKVPVIDPLPMLCPGGWCRATEGDNLIYRNQGHLAATFTREKYGWLG
                     HRLGDPWR"
     gene            complement(21564..22151)
                     /locus_tag="PLJ64_00985"
     CDS             complement(21564..22151)
                     /locus_tag="PLJ64_00985"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HNC92098.1"
                     /translation="MSNNNSPEDFPNFLNNPVETVAERLLGCFFERNLGGRRMLARIV
                     ETEAYDEGDAASHSHRGRTARTEVMFGEAGYLYVYFTYGMHYCCNVVTGEAGHGSAVL
                     IRAVEPVEGIDSMEERRGLHGRNLTNGPAKLCQALDIDLRLGGHDLREEPLRLLTGEL
                     LPGESSTCTTRIGISKATELLRRHYIAGNPWVSRH"
     gene            22238..24115
                     /locus_tag="PLJ64_00990"
     CDS             22238..24115
                     /locus_tag="PLJ64_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92099.1"
                     /translation="MTLIIGVGRSEAHIYWGDTAGDQISRAENDGTGVDSDFLWWSVG
                     NPTAVAIDGTGIYWANNTDNSVRRANIDGSGETLSAWIPTGITSAWGVAIDSTYAYYT
                     NIAGTSIGRKNRNTGVLDQAFITNTGLQPDSVTVSGSYIYWSHSGNAIGRANIDGTGA
                     DPDFISGLSGVRGIAVNGSYIYWAGDTTNGIGRANLNGTGVNQSFITGAATTDGIAVD
                     SSHIYWTLPGPGRIARANLDGSGANTSFIQNAGNPVAVAVDSGVSPPTLTINSGPSGP
                     TSNRTPSFGFTSSGVTLTCSIDQGTPAWTPCTSPYAPPESLADGDWTFRVHAVSSLGS
                     EADQTRTFTVDGQPPETILDGTPNQFTNESTAYFDFHSTEPGSYFECDGGDDDWLLCS
                     SPATIVQVSPGVSTFRVRATDSAGNVDPTPATFEFEMDQIKPETTIDSGPSGTITTSQ
                     ADFAFSSNKSPAQFFCSIDGADAEACASTKTYTGLSDGQHEFRVYARDKFGNQDSTPD
                     SRTFTVDTSGPVDPPSNAFTFGRLKLNTKQGTATLQVKVPGAGKVQLLGSKTVAASSK
                     SARAKSTVTLAVKARGKATKTLKKRGKVGLAVKVRFTPTGGTAKTMTKTVKLVRKKTG
                     K"
     gene            24250..25158
                     /locus_tag="PLJ64_00995"
     CDS             24250..25158
                     /locus_tag="PLJ64_00995"
                     /inference="COORDINATES: protein motif:HMM:NF039218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH hydrolase domain-containing protein"
                     /protein_id="HNC92100.1"
                     /translation="MSGAIGEAGFIPKLDRVKFDDGEVYRQGCIVGDGQLRSRPCRFG
                     YRNSKRRVVLFGDSHAAEWGPALLRLARADHWQVIVLTRASCPAALTNIDRYCNTWRH
                     NSLKRIRRLRPGLVVVASAANYHHYWTLRKRRGLDRQASERPLVHGMIRTLRYLKRWS
                     RKVVLIRDHSAAPFSVTDCLRRNPTRPQRCDFRSTRRKAWSYEFKAAGRVDGVGVIDP
                     QPMLCPGRRCYAVDGRHLVYRNYGHLAAAWVRTRNRWLGRNLNDPWNGRDVKPVLSND
                     SSDERSRPGYLVRSIPSPMNGQATSD"
     gene            complement(25193..25504)
                     /locus_tag="PLJ64_01000"
     CDS             complement(25193..25504)
                     /locus_tag="PLJ64_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92101.1"
                     /translation="MGVAACAVLLIAGCGGVAELDSSETDTVNAANSAVADATASGSL
                     KPGDQAKLEALMVLCHEKPLAESDGESMRELITRLAPELKSADPKMSRRLKRFATDGC
                     N"
     gene            25632..25847
                     /locus_tag="PLJ64_01005"
     CDS             25632..25847
                     /locus_tag="PLJ64_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92102.1"
                     /translation="MGVQTSKTPHTVRLEEASRRAHEAEANGDDAAAIAAWREYRLVR
                     DAGRPAEDLLEEGITLSKMADRLIEPR"
     gene            25844..26320
                     /locus_tag="PLJ64_01010"
     CDS             25844..26320
                     /locus_tag="PLJ64_01010"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNC92103.1"
                     /translation="MTDFGRILKDLNAAGVRYVVVGGIALIRHGVVRATRDVDVVYDP
                     DPSNLEAIRSLAEKWGATRPDGSPLLENAFSAGRSVHLSTPLGEIDLLSEETASVSFV
                     NLLERSEIRRIDAVEAPICSLADLVALKRAAGRERDLADLSDLEATHGSLPARPED"
     gene            complement(26409..26497)
                     /locus_tag="PLJ64_01015"
     tRNA            complement(26409..26497)
                     /locus_tag="PLJ64_01015"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26461..26463),aa:Ser,seq:tga)
     gene            complement(26563..26742)
                     /locus_tag="PLJ64_01020"
     CDS             complement(26563..26742)
                     /locus_tag="PLJ64_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92104.1"
                     /translation="MSKMGEVESEEEIRKEMEELGVSNDATGSPDVLMSGPMIQVTVI
                     VLVVLLVAGALFLVF"
     gene            26781..27962
                     /locus_tag="PLJ64_01025"
     CDS             26781..27962
                     /locus_tag="PLJ64_01025"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC92105.1"
                     /translation="MRRLLFLSCAVVFLDVTFFSVLTPLLPSYKSDHHLSEGAIGVLA
                     GAFAAGSLVMAVPGGWLVARVGARKTVIAGLTGIGICSPLFGFAEHIVLLDLLRFLQG
                     GCGALMWAGAISWVVVAAPTARRGELLGIVIAAAVVGELLGAPLGAIAHQVGTEPVFS
                     MVLVAALILITIAVTLPEPTGVESQPLGEAWRRIRQSDVPSGVLMLAGPSVAFGLVVV
                     IGPLRMDDLGASPFLIAAAFASGSVVEAVVGPVIGRISDRVGRTLPYLIGAGALILAL
                     VGLGIFDRLSLLFAVVMVMSFGAGMAFTPASTLVTDTATAAGVNQGYASGASNVAWGG
                     GQMIGAIGGGALAGAAGYLLPCLVAAAVMAGVGIVARSLGIGPPVISDLSEEAGTADG
                     R"
     gene            27952..>29152
                     /locus_tag="PLJ64_01030"
     CDS             27952..>29152
                     /locus_tag="PLJ64_01030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HNC92106.1"
                     /translation="MVGENYDARKLGSGGHAWTNWAGHQSCRPRDLIRVSGVGELAEV
                     VGAAGAAGRQITTVGSGHSFTPTALSDDLLLDISRISGVISADRDSGLVEVGAGTVLA
                     DLNRELDHLGLAMPNLGDIDRQTIAGAVSTGTHGTGRALPNISAQIEKARIMGADGEI
                     RELEPGSDGLLAARVAVGSLGVLVSVTLRTVPAFNLHRVDAPMDLDQVLAGFDRITEE
                     NEHFEFFVFPYTRTALTLIRNRTDQPLAPRSQADRFLGDVVVENGLGDLALRLTGRFR
                     SAIPKMARFSTGFMSQSEQIDVSHRVFANYRTIRFNEMEYALPAEHGPEALDRVLELI
                     ETENFPLAMPIECRISAGDDALISQAHERPTVYIAVHQHRTMEYEPYFREIERIFREY
                     DGRPHWGK"
BASE COUNT         4890 a         9546 c         9656 g         5060 t
ORIGIN      
        1 acatttgacc gatcctctca aagcaattgg cctccaacaa acccggggcg gttcaattcg
       61 cgacgggacg tacttggtga caagcgaagt tgagccaccg agttttttcg cgacagctcg
      121 aacagtaact ccacgggttc cctcgtcacg aacgagttga agggtcgctt ctgcaatcgc
      181 cgacaggcgc tcttgaacgt tgatttcagg tggcactaac ggcagcctac gcggtcgttt
      241 ctccgcgtag gctgccgaca ggccgctctt acctggcagt agaaccgtca ggccccttgc
      301 ccgtgggaat cgagaatgtg ttctcacggc aaggctgccg cagcacggtg tcacaggcag
      361 gcttcggtgt cttgatgccg ccgaccctca tcagtccaag cacgagcttg gagttccggt
      421 tcggtccgtg gaacaccttg atcttggtgt cgtcccgcgg gccttcgaat ccgtatccac
      481 cggtggtgct cggcgtgtca aggagaaccc tgatgctcgt gcccttacgg aagatgtggg
      541 cgaactgcat gatctccact cgtccgtaga cgggcttaga gtccttcagc atctgaaccg
      601 cgcccttggt cctcttcagg aacggaaggg tgggggtcga gagctttttg tcgagtgccc
      661 tttcggagag gcgcagccaa ccgcggttga cgtagacctc cttgccgtca ggccggacct
      721 cggtgatcgt ggcctgaatg tcggcgtccg gagtcgttgc cgacacccag aggtcaacgc
      781 tggccgggcc ctggaacgtc agagtccgct ggagcggagc ggtggtgaaa gcgaccgttc
      841 cgttcgggtc agcaggatcc tcttgccaat ccgtgtcgcc gggttcggcg tagtcaggat
      901 tgacgttcgg gctcaccgtc gggaagtcat acgacgaggc cggttccggc ttcctcggct
      961 tgttcgtcgt caggcttcca cttgatcgaa gtgacagcct aagcggcttg accttgatcg
     1021 gcaggctctt ctgggtcagg acagttccgg gggtcgactt tcggaggttg ccactcttgg
     1081 tagaagatcc atccggggtc ttcgtgtcaa accacaggcg gacgtgcggc ttcttctcga
     1141 agccgttctt cttgcccttt acgtagcgat cgaagaacgg aatcagcatc gcctgccagc
     1201 ggtctgccat gtagatgtcg tgcgggccgt tggtgccgac gaggtaggac ctcttcggat
     1261 cgaggcggtt ctggtagtag cccgctcgag gtccggtcgc ttcgtcctgc caggcaacca
     1321 tcgagatgac cgcggccttg atcttgtgga cctgacgcca gacgtgattt gtccgaccgg
     1381 gtttgttgtc gacatgtgga cgctcgacac cctctcgata ggggctgacg gcgttgaaac
     1441 cggcattgtg ggcggcgatg ttgtcaatgc acttggtgtc gccttcaact tctgcgctgg
     1501 tcttggcggc gttccacctt tgctggatga agagacccca gccgtagggg aatagccagt
     1561 tggtcacacc gcccgggctg gcgacgtcgc gccactgatc ggtcacggcc atgccgggag
     1621 cgatcgcctt caggtgcgga ggattgttca ccgcagtcaa gatctgactt agccccgcat
     1681 acgaccaatt cgccattccg actttgccgt tcgaccacgg ctgatcggcc gtccactcga
     1741 cggcgtcgta gccatccgtg ccccattcgt tcgcgaacaa gctgaagttg ccgtccgagc
     1801 atcctgttcc gcgcatattc acgccgacca ccgcgtaccc agctttgagc agcttcgcgt
     1861 caagttcagg gtccatggtt gtattgccag cttgataggc ggtgccaccg atgcttccgg
     1921 ggtcatagcc cgagtaattc atgatcgccg ggaacggtcc gtcgccgggt ggaagaagga
     1981 cgctgtattt caggtccata ccgtctcggg catccatgta tccggtcaag cgctccgccc
     2041 agaccccatt gacgggcagg taattaccgg gcttgggaag gtcggcccgt tgactgtcag
     2101 gcaacgggtt tgccgcttgg gccagcgagc tgaataccag cagtccgacg actgccaaag
     2161 caattgcgac tgaccaagcc gaagtccgct tcaggaccag gagtccagct tcagtttgca
     2221 ccacggtaac gcactcctcc tcctagacaa caatgttgtc taaccataca caacgctgtt
     2281 gcctgaaagg tagtttgatc ctgtacctgg tcttggccgt ccacttgaac tgaggtattc
     2341 cgcccgcagc aggctggtgc gacgcccagt atccggaggg cgcgcttcgg gatatgggcc
     2401 gggttcagac ccccgggatc gggcccgcct ggacctccat ccagcgggcg gccatcgccg
     2461 gggggatgac ccggttcgca tcccggttcg tcttcaggca gacaaccgaa ggccggccgg
     2521 aatcacggca ccggaccagg gcctcgccca ggtctccgag ttgatccacg aattcgccgt
     2581 ggcatccgag cccctcggcg accagatccc atcgggtctc gccctgctcg gtaccgaagg
     2641 tccggttgaa gagggcgagt tcgtttggtt cctccatggt ccaggagccc tcggcgaaga
     2701 cgatcacggt gaccgggatc gagtcgcgcg cggctgactg catttccatg atgttgaacc
     2761 cggcggcgcc gtccccggtc acgcagacga catcccggtc gggacagccg atcttcgatc
     2821 cgatcgcgga aggaatgccg gttccgagca tgcccagctc gaggatgctg tggtacgaac
     2881 ggggcggggt cggcggcagg ccggcgtagg cccagaccga ggtcaggccg ccgtccaccg
     2941 tgtagatcgc ctggtcgcca aaggtctcgc cgatcacctc gatcgcccgg gccgggtgga
     3001 tcccctcgcc ttcccagtca tccgcggtgg aggcctgaac tgcctgccag gccgcgtcag
     3061 cttcccgggc ctcggccagg gccgcggtcg cttcggccga gggagtcttg tcccgtcttt
     3121 tcagttcggc tgtgatcccg cccagcgcgt cggcggcgtc ggcgaccatg ccgatcgcca
     3181 ggggccgggt tacaccgaga tggctggggt cggtatcgat ctggatcagg ctttggttct
     3241 cggggtcgcc ccagtacttg tcgtagggga ggtcgaggtt gccgatccgg gacccggcga
     3301 ccagaatcac atcggcttcg agccgggcct tgatcccgcc ttcgccgaag ccgaagacat
     3361 agttcgggtg gtcggatctg acgctggccc gtccggcctg ggtggggatc agcgggcagc
     3421 cgagcagctc ggccagttcc gccgcctgcg cgttggcccc ggcccggtcc acgccggctc
     3481 cggccaccac gatcgggcgg ctggccgact cgagcagatc cgcggcggca ccgatctgcg
     3541 cggctgatgc ctgtggctgt gaagggcggt aggccccggg ggcgaggatc ggctgctcgc
     3601 tctcttcgcc gaggtcatac atgacggtga ccgggacatc gacgtgaacc gggccgggac
     3661 ggccgttcca catctcgcgg aaggcgagcc ggagcacctc ggggatccgg tcccattcga
     3721 agatcggccc gccccacttg accgcgggcc ggaaggcatc gagctggtcc tggccctgga
     3781 aagtcgaagg tggcgagggg taactcacgc ccatacggtg ttgcgaggtg atcgccagca
     3841 cgggcacgcc ttcgtgaagt gccgtgaaga tgcccggcag caggttggct gacccggggc
     3901 cggggttgcc gatcacgcag gacgccttgc cggtcgactt gtagagaccc tcggccatgt
     3961 ggaccccggc cgattcatgc cgcaccggca tcagccggat cccgtacttc tcgagctgcg
     4021 cgaagagggg gtcgatctcg ggggaaggca atccgaagac atgctctatt ccctcgcttg
     4081 ccagacactt ggcgaagagt tcgccgccgg tgatctcgga catcactgct ccttgctgtc
     4141 ggcggcatcg tgcggtgccg cacgggattg gaagtctgct tttcttccgg aaaagggcag
     4201 acccgagtgt aggagcaaat ctcgtcttct ccgcttcccg agcgaaagtt cagggttttc
     4261 ccgcagtctc ctgaagggcg ggagcgaggc tcagggcagc gggatcttct tgcccgggtt
     4321 gagcagcagc ttggggtcga agactcgttt gatctccgcc tgcagccgca ccgcgaccgg
     4381 cccgagggca gcctccacat gtccggtctt gagcgagccg atgccatgct cgccgctgat
     4441 cgacccgccc aggcggatcg ccatctcgaa gatctcggcc gccgccgcgc cgccccgctt
     4501 catctggtca tcgtcggcca ggtcgacgag gaagctggaa tgcaggttgc cgtccccggc
     4561 gtgaccccag gaacaggccg gcaggtcgtg acggcggccg atctcgaccg tctcgtcgac
     4621 cgccgcagcc agcgactcga ccggaaccac gacatcctcg gaaatcttgc cgccgcgctg
     4681 ggcggtgacg gcgatcgaaa ctccgtcccg ccaggcccag aagttccgga ttgcgacatg
     4741 gtcggtgatc ctgaccggcg gctgaagtgc ccctccggcc atcgcctctt ccaggtcccc
     4801 gaccgcctgg ttcacctcgc cggcggaacc gtcggcctcg gcgatcacgg caaaacccgc
     4861 ctgctccggt gcctcggaag gaaaggaacc gaggctggat tcgagcgcgc cgcggtcgag
     4921 gaactcgagc gccgtcggct ggatcccgga acccatgatc attccaatcg cctcgcagcc
     4981 ggaggcggtg tccgggtaga agccgaccac gacgccgcgt tcctcggggg ccggcaccag
     5041 ccgcagccag gctgaggtga cgatcccgag ggttccttcc gaaccgatca tcaggtcggt
     5101 caggtcgagg ccggcgacgt tcttcctcag cggcccgccg gaggaaagga cctctcccgg
     5161 tggcacgacc gcctcgatgc cggtcaccca ggccccggtc accccgtacc ggaatgcgtg
     5221 agggccgccg gcgttggtgg cgatgttgcc gccgatcgtg gactgttcgc tggcccccgg
     5281 gtcgggaggg aacatcagtc cgttctcgag cgcgatccgg tgcactgtgg ccgtccggac
     5341 cccggcctcg acttccatcc gccagagctc gggatcgatc gaacgaaccc gggctagccg
     5401 ttccaggctg agtacaaccc cgccatccgg cacggcccca ccggccagtc cggtgccacc
     5461 cccgcgcacg gtcagcggca cgccatgccc gtagcaccag gccatgacct cggccacttc
     5521 ttcagcgtcg gcgggcagga ggagagcctc ggcctcgcct ttgatgccgc ggttgccggt
     5581 tccgtcctgc agggcagctc tcaattcggg gccgatagtg accacctgtt caggcgaaag
     5641 caggttcgcc agttcccggg caaggttgcc gttcacgatt cgcttttcag aaattcctga
     5701 ttccaatgcg ggttcgtccc tcagctcccc ggatcctgat tgcaaggtag ccggtagtag
     5761 gtaatatgtt acctgctata gcgtgggcat cagcccggag gaaggagaac aggtgggact
     5821 gaccggagga gaattcgtcg ccgagtatct gatcgcggag ggggttccgt atgtattcgg
     5881 aattcccggt catggcgaca tggcggcatt cgatgcattc aaggaccggg ccgaccggat
     5941 caccatgatc ggcccgcggc acgagcaggc ggctgcccat atggccgatg cctattaccg
     6001 ggcgtcgggc aaacctctgg ccacgatgac ctcgatcggt ccgggttcga tgaacgtggc
     6061 caccggtctc gggaccgcct tcgtcgattc gatcccgctg atctcattca ccggaggccc
     6121 ccagacctac atgctcggcc gcggtgtcct gcaggaactc gaacgccagc aggacaatgt
     6181 cttccctcac atcatccagc cgatggtcaa gcggaactgg gatgtccagc atgtggacct
     6241 gctggctgat gttctgccgc gggccttcaa caacatgctc tcgggccggc ccggtccggt
     6301 tcacatcgag ctgccgatgg acgttcaggc cgaagtgacc gaactgccgg ttccggaccc
     6361 ggccgagcgg cgggccacat ccggcgcggt gaggccggac ccggacgcga ttgacaaggc
     6421 ggcggcgaag ctgctcgcgg cgaagcggcc ggtgctgctg gccggcggcg gctgcctcga
     6481 ggcgaaggcg gcggccgagg tgaagaaggt cgccgaatat ctggacgcgg ccgtggtcgt
     6541 gaccatgatg ggcaaggggg tcttccccga ggaccatccg ctcgacgccg aacacaccgg
     6601 ctccaacggc accctggtcg gcaactacat gaccaagaac gcggacgtga tcctcgcggt
     6661 cggcacccgc tttgccgagc agacctcctc ctccttcatg gacgggatgt ccttctcgat
     6721 tccgccgacc gagatcatcc acctcgacat cgacccgacc gagatcggca agaactaccc
     6781 gaccgcggtc ggcgcggtct gtgacgcaaa ggccggtctc aacgatctct tcgacgcgct
     6841 ggtcgatcac aacggaaccg aaggaatcag ccggcccgcc taccgggaag agatcgcgaa
     6901 gctgcgggcc gactggaagg aaatgctgac cggccgctgg accggcaagc tctcgctcag
     6961 ccaggtcctg cacaagtcac gggaggtatt gccgcgggaa gcgatcgcga tcgcctcggc
     7021 cggccacccc cagatccagg ccttccagga gttcctctcc tatgaaccgc ggacctggct
     7081 cagcccgggc ggttactcga ccatgggcta caccctgccg gccgcgatcg gagccaagct
     7141 ggccaaaccg gatgtgccgg tggtcggctt cgccggtgac ggtgacctcc agcagacgat
     7201 ccaggagctg gcagttgccg ccgagaccgg cgcggccgtg atcatcgcct gcctcaacaa
     7261 caccggctgg atctcgatcc gggacttcca gcgcggcatg ttcggcgagg accgcggctt
     7321 cgagaccgag ttccgctcgc gcaagggcga ccagctgatg ccggtcgact tctgcgcgat
     7381 tgccgaagcc ttcaactgcg gctcggagaa ggtgaccgag accggcgata tcggtcccgc
     7441 cttcgagcgg gcgatggcct cggaagggcc ctgcctgatc gagttcgaac tctcatccga
     7501 accggccgac agtgaaggcg tgaacatcgg ccactgggat ctgccgaagc cggcctacct
     7561 cccctgaccc ccacccaagg aggcaacaag gtgaatcacg acacccccga aagagacccg
     7621 aacggggccg cgcaacagct gaagaacgag tccgatgacc atgtcatcaa gtgcatgggc
     7681 gatgtggaca tgggcctggt caagggcgaa ggcgtccggg tctgggatgc cgacggcaac
     7741 gaatacctgg acgcgatctc ggccgaatgg gttctcaatc tcggcttccg ccaccccgag
     7801 atcatcgaag cgatggaggc ccagttcggc aagatcgact atgtatcgcc ggtcttcgag
     7861 gatgaagccc ggaccgccta tgcgaagaaa ctggctgaac ttgcgccagg agcactctcg
     7921 aaggtgctct acggtctctc cggcgcatcg gccgtggaag gagcgatgca tcttgcgatg
     7981 cggacgacgg gtgggactga tttcgtctgc ctcgacgctg ccttccacgg ccggaccttt
     8041 gccacgatcg gcctgaccta cgtgaacccc ggcatggtcg agggggccaa caagggcctc
     8101 gaccgctacc tgccgcgaca gatgcgggtg ccgaattaca actgctaccg ctgcccgctc
     8161 aacctggaac ccggaacctg tgacatggcc tgcgcggaag cggtcgactg ggccctcgac
     8221 aagggccacc tgaacggtcc ggccggggtg atcgtcgagc cctaccaggc aaacggcggc
     8281 atggttcccg ccccggacgg ctacctcgag cgggtccacg agatcgccaa gtcacatgag
     8341 gtcccgctga tcgtcgacga gatccagggc gctctttgcc gctgcgggcc gatgtacgcc
     8401 agccagcggg ccgggatcga gccggagatg atcatcctcg gcaaggcgct tggtggcggc
     8461 ctgccgctct cggccacgat caccaccccc gagtactcgg aactgctgcc ctgggaatac
     8521 ggcttcaccc aggccggcaa cccgatggcc tgcgcggcgg gcctggcaat ggtcgagatc
     8581 atggtccggg atgacctgcc tgccaattcc gagcggatcg gcacgatgat gatcgaggaa
     8641 ctggagaaga tccaggagga ctcgaagctg atcggcgatg ttcgcggcca gggcctgatg
     8701 atcggggtcg agctggtccg cgaccgggag accagggaac cggccttcga ggaaaccgac
     8761 cagctgatgg ccgcctgcct cgagcatgga ctgatgatcg gcaagaccgg cccggtcttc
     8821 ggcgcgaacg gcaacgtcgc caagttcaag ccggccgtca acgtgaccga ggaagacgcg
     8881 atggagatga tcgacctctt caagcgggcc ctcggtgatg tcgaggcgac tctgtgacga
     8941 gggagatcag cttcatcgcg gcgctcgact acgaagagcg ccccgccgcg gaagtcctcg
     9001 agtcgctgct cgaggccgga tatgactcgg tcgagtggac catggcccac gcggaggaac
     9061 tgctcgcgcc ggcctcggcc ctcgcctgtc agatggacct ggtcaccgga ggcgaaaaag
     9121 cggccgagga aaccatccgc ggcatcgaag cggcggccgc cgccgggatc ggggtcgtca
     9181 acgtgctgac cggcccgaat ctgtgggagg agggcgcggt tcagcgcgac gatgaagcag
     9241 cctggaaggt tgcgctcgaa gcgctggagt ggatcgtcgc ccggggcgag gcggcgggag
     9301 ttgcgatcgg attcgaaccc tgctggggca ccctggccca cgacgcgcag accgcccagc
     9361 gggtgctgga ttcggtcggc gtttcggtca ccttcgaccc cagccatttc gtgatgaccg
     9421 gcgacccgat cccggagctg atcgaacgct ggggcgagcg gatcgtccac ttccacctca
     9481 aggacgcctt cggccggccc ggcatcgacg gcgaggactt catcttctgc atgctcggtg
     9541 agggcaaggt tccctggccg gagactttcg cggcgctcga ccgagtcgga tacgaaggcc
     9601 cgctctcggt cgagttcgag gcctacgctt attacgagca ggtgctgggc agcgacccgg
     9661 ctgccgcggc caggctcgcc cgcggccagg tggcggccct gctcggggag ggctcgtgaa
     9721 ggtcgcgctc tgcggactcg gcgagatcgg ccagatccac ctcagggcga tccgcgaaag
     9781 cggtcacgaa ctggtcggtg tgctggaact gaaccgccgc ctcgcggtcg aaagcgtcgg
     9841 tgacgaggtg ccgatccaca ccgaggtcat ggagatggtc gaactgaccg agccggatgt
     9901 ggtcgacatc tgcctgcctc accaccttca cgtgccgatc gccgtggccg cgctcgaagc
     9961 cggcgccgac gtgctgctcg agaagccgat ggcgattgat ctcgccggct gtgacgagat
    10021 catcgccgcg gccgaagcct ttgaccaccg ggtcgggatc tcccacaacc agatccactt
    10081 cgcgcctcac cggcgtctgc gtgagctgat cgacgcgggc cggctggggc agatcagggc
    10141 gatctacgag cgcctgtgga tgggcggaaa gtacggcggc tggcgggagg attcatccca
    10201 ggttggcggc gggctcctga tggacgcggg ggtccaccgg gtctacatgg ccgagtatct
    10261 cggcggcccg gtaagcaccg tttcggcggt gatggacaag ccccggtcgg aggacagctt
    10321 cgtcctcacc ctcgagttcg agagcggagc caccggcgtg atccagggtg gctatcacgg
    10381 gcccgaaggg gtcttcgacg accggatcga gatccagggc tcggccggga tggccgaggc
    10441 gctgggctgc gaggcgttct tcgaaggtga cctttccggg gacctcaggc tgcgggcccg
    10501 gctcgacgga gcctgggtcg atgacccggt gaccggcgac tgggacgagt cggtgatcgc
    10561 ctcggtccgg gcctgcctcg acgatctcgc cgcgggccgg gaacccgagt cggggctggc
    10621 ggcgggccgc tccgcagttg ccgtaatcga ggctgcctat cgttcggcag agaccggcaa
    10681 ctcgattcgc gtcagcgatc tggatgggag gcagtgatga agagcatcga catcgggaac
    10741 gaggaaaagg gatcgctgcg ggaaatcaag gctgaatcga gcctcacggc gagggtgcgc
    10801 gagtcgattc gcgaggcgat catcgacggt agcctggagc ccgggtcgct tcactcggtc
    10861 aagtcgctgg ctgacatctt caaggtttcc cggaccccgg ttcgcgaggc cctgatcgac
    10921 ctcgccgggg ccgacatggt cgagttcgag cgcaatcgcg gtgtccggat tctcgagacc
    10981 tcggtccacg acctcgaaga gatcctcgtc ctgcggatcc tactcgaggt tcccgccacc
    11041 taccgggcgg ccgagcacat cgatgccgac gggctcagcg ccctcaggca ggagctcgac
    11101 gcgatggtcg aggcggccga gaacggggac gagccgacca tgatgcagca cgaccgccgc
    11161 ttccacgaac tgatcaacac ggcctccggc aactcccggt tgaccgaata cgtcgactcg
    11221 cttcgcgacc tgatcctgac ccgcggagtt tcgaccgttg acaacacgcg ctcgctgacg
    11281 gagatcgtcg acgagcacga agggatcctc accgcgattg cttccggcga tggtgaagcg
    11341 gcagcggccg cgatgaagga gcacctggtc aacacggcct cgctcctgct tcagcaggag
    11401 ggcggacgca gctcgtcgct gagcctgggc tgggagaggc tggtcgccgg cggatgagca
    11461 agctggtcaa gctcgcggtc gccagctggc gacgccagaa gctccgcacc gcgatgacgg
    11521 tcggctcgct ggccctttcg gtcggggcgg tcgtcttcgt ctattcgctt tcggtcgctt
    11581 tcgagagttc cggctcccag gcgatcgagt cggcgatcgg caaggccgac atctgggtgg
    11641 tgccggccaa gggggtcgag gtcaaccgga aggacgagag catcgacgcg atcggcagcc
    11701 tgccgtcggg gatcgaggcc gaagtcagat cgctgccggc agtcactgcc gtgaccgcgg
    11761 tcaccggcag cggtgccgga acccttcagg tcaccacttc caacccgaag ctgaccgcgg
    11821 acgcgatgaa gagcaagggc ctgaccgtga cttcggaccc ggctcgcagc acggcgaccg
    11881 gagacgcccc ggccctcgcc tacctgatca cggccagcga ggaccgcttc ggcgtctaca
    11941 ccttctccca gcagttcgag gcggttcagg tcaacgaggt ggcctccagc gtgctcgggg
    12001 tggtcggccg ggtcaccctt gcgctcggtt tcctcgcggt cctgacctca ctgttgatct
    12061 cgatcgacga acgccgccgc gagttcggca tcctcgccgc ggtggggatc accgatgacg
    12121 tgctctacct gtttctggtc gaatccggac tcctgatcgc gatggggctg gtggtcgggg
    12181 tgatcttcgg aggaggcatg ttcgccctgc ttttgccctc gatcttctcg ctggggacgg
    12241 tgggcaaggc ggttgccctg gtctcggtct acttcccgat catgctcatt ctcggggcgt
    12301 tgatcccggc ctggcgcctg cttcagaaga gtcccctcga actgctcagg agttcacctt
    12361 gatcaaaccc ctcaagtaca gcctgcggat gatcacccgg gcccgcggct cgtcgctgct
    12421 tcgctccggc gcggtggccg tcgcggccgc cctcaccttc ttcgtcctcg cccagagttc
    12481 gaccgttcgc aatcaggcca ccgatctcgc ggtcggcggg atcggggtgg acacgatcga
    12541 gagggcgacc ctttcgctga ccgtgatcgc cgtgatcgtc ggcgccctcc aggtcggcgt
    12601 gctgatgacc cgggtggtgc tgcagcggat gcaggaaatc ggcgtgctca aggcggccgg
    12661 agtatcgagc cgggacatct tcctcgtctt tctcttcgag tccctgatct acggcttcac
    12721 cggcggggtg atcggctgcc tggtcggtct cctgatcgcc ctgctcggac cgatgccggg
    12781 cagcgaggag attctgcggg ccttcgtgat cacggtcgcc ctttccaccg tcgttgccac
    12841 cctggccggg ctggcgcccg cccgtcgcgc cgttcaggcc ccggccgtag aagcaatcaa
    12901 ccatgcctgg tgagatgaat gccgccggct gaaggagatc cgatgaacga acacgagcaa
    12961 gtcccgggcg gcaccgggcc ggtggtcgag accgaagacc tgtggaagct gttcaagctc
    13021 ggcgatgagg tggtcaaggc gctcaagggc gtcagcctga agatccaccc cggtgaactc
    13081 gtcgtgatca tgggccccag cggcagcggc aagtcaacga tgctgcacct gatgggcggg
    13141 ctggagcggc cgaccaaggg cgagatctac ctcgaaggcc agccgctctc cgggctgacc
    13201 gagagccagc tctcgaccat gcgccatcag gcggtcggtt tcatcttcca gtcctacaac
    13261 ctgatcccgc tgctgaccgc gaccgagaac gtcgaactgc ccctggtctt cgacgacgtt
    13321 gacagcggcc aggtccggcg tgactgcgtc gagctgctcg agcgggtcgg cctcggtcac
    13381 cggcttgatc acactccgag ccgcatgtcc ggcggcgagc agcagcgggt ggcaatcgcc
    13441 cgggccctga tcggcaagcc gcgcctgctc ttggccgacg agccgaccgc aaaccttgac
    13501 cacaaaaacg gcgacgcgat cgtcaagctg ctgaccaccc tcaaccacga gatgggcatg
    13561 accaccgtga tctcgaccca cgaccgctcg gtggccgagc atgccagccg ggtgatcgag
    13621 atgcgggacg ggaaggtagt ttccgactcc ggttcggggg acggcgcccc ggccgaccgt
    13681 ctcgccaccg cctgaggaag ccgtgacagc gatcagcagc gtcaatccct ttgatccctc
    13741 cgaccaggtt gccggttgcg agggtgcttc cgcggccgaa atcgattcgg tcctcgccgc
    13801 cggggtggcg gcccagccgg gctgggcggc cgacccgatg ctcaggctgc gttccctttc
    13861 gaccctcgcc gacgagatcg aagtccggcg ccagaagttc atcgacctga tggtgcggga
    13921 agtcggcaaa ccggtcgccg aggcggccgg cgaagttgac cgggcgatcg cgatcctcgg
    13981 cttctattcc caggttccgc tcgatccggt cggggaggtg gtcccgggct cggtgccggg
    14041 cgcccgggtt ctggtcgagc gccatccact cgggctggtg ctggcgatct gcccctggaa
    14101 cttccccctg gcgatcccgc tgtggaagtc cgctcccgcc ctggcctacg gaaacgcggt
    14161 cgtgatcaaa ccggcttcac cggcgatcgc caccgccgcg ctgctagccg aatgtgcggc
    14221 cggggcgctg cctgaagggg tgctgtcggt cttgccgatg agcggggccc ggacctccga
    14281 ggtgctcgat gacgaacgga ttgccgccgt caccttcacc ggctcgaccg gggtggggat
    14341 ctcggtcgcc gagcggatgg cctcccgggc ggcccccgcc caggccgaga tgggtggcca
    14401 gaacgcggcc atcgtcctcg aggacgcgga ccttgacgcg gcagccgacg cgatcatctc
    14461 cggcgcgatg ggtttcgccg ggcagaaatg caccgcgacc cgccgcgtgg tcgtggtcag
    14521 tcaggtccgg gagcaactgg aagcaaagct ggtcgaaagg gtccgggggc tctcggtcgg
    14581 ggacccggaa cagagcggag tcacggttgg gcccctgatc agcgaatcgg cggtcgagga
    14641 gttcgaggct gcccgttcgg cggcgctcga ggcgggtgcc acggagcttg ccgtcgccga
    14701 ggcgccggag ggcgagggct atttcgtttc gccggccctg cttcgccagg acgacccggc
    14761 ggcgacggtc aaccaggagg aaactttcgg cccgctgctc accctgatcg aagtggccga
    14821 cgaggacgcg gcggtcgaag ctgccaacgc cacccgcttc gggctggtcg gcgcggtcca
    14881 cggccgggat ctgggccggg ctggccgggt cgccgggcgg atgaccaccg gcctccgccg
    14941 ggtcaacgcg ccgaccccgg gggttgattt ctacgctccc ttcggaggcg agggccagtc
    15001 ctccttcggc ccgagggaac agggtcgcgc cgcccgcgag ttcttcacct ccagctcgac
    15061 cacgacgatc ctgccctccg caacctgacc gggggatgca tcgagatgca cttcgtcggc
    15121 gttattcgcc gaccaagtga atctcgcact tctcccgttt cacaaggttg aagttatccg
    15181 cgaatagcgc acccaactcc gatctcgatc ccccacacgc tcgcgctacc gctcgcgcag
    15241 cagggcaatc ggacttacat caagccgaag atcgtcccga ttgccctatt gcctgcgcaa
    15301 aagggcaatg ggcacgatcg ctacgagcag gtagagggcg aggccgattt gctggcttac
    15361 tgcctgggtg gtggtgaagc cggtcaggtg gctgacgtgg aaggcgaagt gcaggatctg
    15421 ggcggcgatc cagccccagc agagcggcag gacgagttcg cgcgaaggtt tgatcgtggc
    15481 ccagatgaac atcaccccga agcccaggta gagaccaccg acatcggtga tcagatgctc
    15541 gttgtagggc ggcagcagtt tgaccagcgc cgtgtagaac ggatagtcgg tgtagaaggt
    15601 ctccggggca aacgaggcgg gcagggcgat cgaaagagcc gagagcccca gcaccgcgag
    15661 gcagatccgg atcgccggcg agtcggacgg cgccggcttg ccccgtttcc cggtcgccgg
    15721 cttgcgggcc ggctttctcg cgccggtctt cccggccgac gctgctatcg cccgaacctc
    15781 acgacctgat tttccatcgg caggacccgg cggtagacgc cgcgcaggcg gttgtggttg
    15841 atgctcgcga cttcgccatt ctggatgccg gccacgttcc cgagcacgcc ctggccgcgg
    15901 aaattccgga agacaaacgg caggccgctc gactggagcg gggagggcga gttcatgacg
    15961 tggaagtgaa ggtggggccc gtcggtgttg ccggtgttgc cgagcagccc gatgacctgc
    16021 ccggccttga ccttctgccc gcgccggacc cggatgctgt tggtggcgag atgggcatag
    16081 aaggcgtacc tcccgtgacc gatgtcaacc accacatggt tcccgcccgc catctggatt
    16141 gtcgcgccgg ccggcagcga acccggaatc tgttcgggca ggttcctccg taccccgacc
    16201 acccggccgg gagcggccga atagaccggg gtgcggtaat agccgtaact gaacaggtcc
    16261 accttcaacc cgctgtagag cctgttctgg tcgttcagct ggacgaagtc gatcgcgaag
    16321 cgttccggta cccgaaccgt gccgtcgatc gccagggtcg cgccacggtg ggcgttcagg
    16381 gtgcagcagc cgttgaccgc cacccatttc gaaccgcgca gcggagccga aagcacgacc
    16441 ggcttctgcc gggccacccg ggtcgagacc ccgaggaagg tgtgtccgac cggctcggac
    16501 ggggtttccg ggtcggccca ggcgaggctg atcgcgtggc tgatcgcctt cggccgtttg
    16561 cgccccaggc tgtaggaagc gtcgatgaag atcatcgcgc tgccacccgg gccgagcgtg
    16621 gttccggtgg ccccggagtt gattctcatt cgctcctcaa ggcggctgcc ggtgaacggc
    16681 aggccgagcc ggttgccctg ggcccgcgga gcaaccttgc ggatctccac ttccagaccc
    16741 gactggttca cggcctggat ctcgtagacc atgtgccgcc ggtcatcggc gccgcgcacc
    16801 ggggtgggcc ggttcacgat ctgaaaatca accggggaga ggaacgcctg ggaatccggc
    16861 cggatcgccg atgccggcga ggcagcggcc accaggccga gcagaacggc agcgaacaga
    16921 atcaggcgag acgacccccg ggttttcatg ccgccagcct atcggcggcg gctgatcagg
    16981 ttgctgcgct tcaggccccc cgcggcaggg aggctgtaga ggtcaccgtc ggcggcgatc
    17041 cagaggggtc cgtcgaaccg gtccggggcg tcggccaggt agatgtcttc gaggcccttg
    17101 aggggcagcg ggggcacgac gtgggtgagg gcaagcgccc tggcgccggc ctgccgggcg
    17161 gcatcggcgg cctggggggc ggtcgcgtgg tagtcgtgca cgtcggcgag gatcttgcca
    17221 cgggccggat tgccggcttc ggtctgtgcc tccgcgacca tcatcagaag ttcgatcgaa
    17281 agcgcatcgt ggatcagcag gtccgtgttc ttcgcggcct tgacgaggtt ccccgaatag
    17341 acggtgtcgg cactgatcac ggcgctccgg ccctgatagt cgaagcggaa accgaccacc
    17401 ggcgcgaccg gcgaatggtc cacctcgaag gcggacacga ccagcccgtc atcttcgagg
    17461 accacgatcg attccccttc gccggggacc gggaactcct cgaccgccat cggcccgatc
    17521 gccgggtcaa catagtcctc gccgtgatgg gcggttcggt agctgcggtc gagccggtag
    17581 gcgtcgttga accccttcac gaccctttcg gccccgggcg gtcccaggac cggcatcggc
    17641 gtgtcggtgc cgtcggccac ccagcgctgg agattgaccg aggggatgcc gccgatgtga
    17701 tcggagtgga agtgggtcag cagcagggcc tcgatccggc ccgggtcgat gcccatccgg
    17761 ttgatcgttt ccgaggcccc ttcgcccgcg tcgaccacaa agaccttctc acccgcgatg
    17821 accagggtgc agggattgcc gcgcttgtcg tcgggcaagg gagagcccga gccgacgacc
    17881 gcgacgtgga gtccgtcgcc gagttcggcg accgggtcgg ccgcgacgat cttcggaagc
    17941 tgcttcttga tcagggctag ggcgattcgg gagcgtaggg tggccatgat ccgaatccta
    18001 tagttgacgc gcgtcaattt ctagtcccgg gggtgcatct cccttgctgc gacccctttg
    18061 accgcctcgg tgagcacctc caggagcggc gactccaggg tccagcgttg ccagtagagc
    18121 ggcatctcga tcgcctggtc cggggcgaac tcgaccaggt ctccccgctt gatctcttcc
    18181 aggcactggc ggtccggcag aaggccccag cccaggccga gcaggatcgc ccgggcgtaa
    18241 tcggcggtgg tcgggatgta atgccgcggc gcccggagcg gcttgccggt gaattcccgg
    18301 aagaaacggt tctgggtctg gtccttgcgg tcgaagtcga ccaccgtggc cttgccgagg
    18361 ttggccggcg tcgcttcgcc gccaagctgc ccttcgagga agccgggtga acagaccggg
    18421 tggtaggtca tggttccgag cagctcggag cggcagccct ggaccgcttc cgcattcgag
    18481 gtcacggccg ccatcacctt gccggccctg agcagggagg tcgtgtgttc ctggtcctcg
    18541 cggaagacct cgaagaccac ccccggcgag tcgacccgcg ccagggcctc catgaaccag
    18601 gtcccgaggc tgtccgcgtt gatcgccaac ggcaccgtca ccgcgccgcc cccgctttct
    18661 tccagggcca gggcgcgggc ggcctcgtct tccagcagtc gcatctggcg gccgtagcgg
    18721 agggcgatgt cgccggccgt cgtcggttcg accggattgc tccgctggac cagcacctga
    18781 ccggttgacc gttccatcgc cttgatccgc tgcgagatcg ccgacggggt gaccgagagg
    18841 cgccgggccg cggcctcaaa ggtcccttcc tcgaccaggg caaccagggt ttcgagatgt
    18901 tcggtgcgga agccggtcat tagcagcgct aaagatagat aagaaagttt agtttgactt
    18961 cacacgcgga cggccccaga atcggactcc gcatgactgc ctccctcctc cagatgatgt
    19021 tgctgggctt cgccgccagc ctcaccctga tcgtcgcgat cggggcccag aacgcctacg
    19081 tgctcaggct cggggtggaa ggtcgcgggc gcatcctggt cacagtgatc ctgatctgcg
    19141 ccttctcgga cgcagttctg atcgccgccg gcgtcgccgg aatcggagct gtgctcgagg
    19201 cggctcccgc ggcgatcgac gcggtccgca tcctcggcgc ggtcttcctg atctcctacg
    19261 gagtgatcgc cgcccggcgc gcgctgaacc cgcaggcgat gacggcgggc gagagtccgg
    19321 ccgacgcgtc gatgaaggcg atcgttctga ccaccctggc cttcacctgg ctgaacccgc
    19381 acgtgtacct cgacaccgtg atgctgctcg gctcgattgc caaccagcag cagggcgtcg
    19441 atcgctggtg gtgggcgctc ggcgcggtgg ctgcctcgtt cacctggttc ttcctgctcg
    19501 gctacggggc ccggctgctg ggaccgctct ttgcccggcc gggtgcctgg cgcgtgctcg
    19561 acgccctgat cgccatcatc atggtcgggc tcggcgccgg cctgatcatc ggcggctgac
    19621 ccggccggcc accggttccg gtccggcgac acttgcccgc aaccgtcagg cggtgtaggc
    19681 tcgcggaccc cgcaggcagg gggagaatcg gcacaggagg taagcgatgt cttcagcagg
    19741 gatgaaaaga atcccgggag cagtggtggc gctaacggtg gcggcgctgg tggtcgccgg
    19801 ttgtggcggc gacgggtcca gcaacaaaac cgaaccggcc gcaagccagg ccgcaaccag
    19861 ccagaccctg aacaaccagg aaggcgaatc cgccgacggg ttcgttcctt ccttcagcga
    19921 ggtcaaacag gacaagggcc cggtcttcgg cgacggctgc ctgattcacg gcgatgagcc
    19981 cgaatccggt gagtgtgcct acggcgacac cgagtcgtcc cggaacgtag tggtcttcgg
    20041 ggattcgcac gcgctccagt ggacgccggc cctgatcggg atcgccagcc agcgcggctg
    20101 gcgcctgacc gccctgctcc atgcgaactg caccgcagcc caggtcagcg tcgacgaaaa
    20161 ctgcgaccgc tggcgggaaa actcgctgaa gaggatcagt gaggaaaagc ccggactcgt
    20221 catcgtggcc agcaacaccg gccccaacat gaccgtcgtg agcgacggca cgaaactgga
    20281 ccgggaggcg gccgggtcgt cgctcgaggc cgggatggcc aggaccttcg aggacctcaa
    20341 ggcgagcggc gccgaggtga ccctgatgca ggacatggcg atgtcgaagg actacttacc
    20401 ttcggaatgc gtcgccgggc accaggacga cccgggccaa tgcaccttcg agatggatcg
    20461 cccgccggag cttgcctatg acctcaaggc ggccagacag gtcggcggtg tccaggtgat
    20521 cgaccccctg ccgaagatct gccccggcta cagctgctac cccgtccacg gccggtacct
    20581 gaagttccgc gaccgcttcc acatcaccgc gacctattca cgccttctcg cgccatggct
    20641 tgactcgcgg ctccagaatc cctgggagga gtgacccgcg agtgaagcat cgcgggtcgt
    20701 cacttctcgc ggggctcctc gcgattgcct gcctggccac cgtcgtcatg gccggtccgg
    20761 ccaccgctcg tttcatcccc ggtctcaacc gggtcagggc tgacaagggc gaggtctacc
    20821 gcaagggctg cctgatgggt cacaaccggg tccggtccgg cccctgccgc ttcggggacc
    20881 gcgactcgaa gaagaaggtc gtcctgctcg gtgattccca cgccatgcag tggggcccgg
    20941 ggctgatccg gctggccagg aagaagcagt ggcaggtgat cgccctgacc cgagccagct
    21001 gcccggcggc gctggtccac atcgactact actgcgacac ctggcgaagg aactcactcc
    21061 ggcggatccg gttgatgcgc cccggcctcg tgctggttgc ctcggccgcc aacagcgatg
    21121 cgtattcggt tttcaccggc ggtggctatc tcagccgcgc ggccagcgag cagtacctgg
    21181 tcgacgggat gatccgcacc ctgcggcgcc tgcgtcgctg gtcgaagaag acggtcgtga
    21241 tgcaggacca ggcggtcacc ccgttcagcg tgaccgcctg cctccgcatc aacagccccg
    21301 ggaattgtgg tttccgggct tcgcgtccgc gggcctggtc atatgacttc aaggcggcgc
    21361 gccgggtgaa gaaggttccg gtcatcgatc ccctgccgat gctctgcccg ggcggctggt
    21421 gccgggcgac cgagggcgac aatctgatct accgcaacca gggccacctc gccgccacct
    21481 tcacccgcga gaagtacggc tggctgggcc acaggctcgg cgacccctgg cgctgaccgc
    21541 ccaggagcgg gcagtccgct ccgctagtgg cgggagaccc aggggttgcc ggcgatgtag
    21601 tgccggcgaa gcagctcggt tgccttgctg atcccgatcc tggtggtgca ggtcgaggat
    21661 tcacccggaa gcagctctcc ggtcaggagc cgcaggggct cctcgcgcag gtcgtgaccg
    21721 ccgagccgca ggtcgatgtc gagggcctgg cagagcttgg ccggtccgtt ggtgaggttc
    21781 cggccgtgaa gcccgcgacg ctcctccatc gagtcgatgc cctcgaccgg ttccacggcc
    21841 cgaatcagca cggccgaccc gtgtccggcc tcgccggtga ccacgttgca gcaatagtgc
    21901 attccgtagg tgaagtacac gtacaggtaa cccgcctcgc cgaacatgac ctcggttcgt
    21961 gccgtcctgc cccggtgcga gtggctggcg gcatcccctt cgtcgtaggc ctcggtctcg
    22021 acgatccggg ccagcatccg gcgcccgccc agatttcgct cgaaaaagca accgagcaac
    22081 cgctccgcga cggtttcgac cgggttattc aggaagttcg ggaaatcttc tggactattg
    22141 ttgttcgaca ttcctactcg agaggcaaga cgctttgacc cacagaatca ggcacatggg
    22201 ggcgatggct gcgtgagcgc ttgccctgct cttcgcattg accctaatta tcggcgtcgg
    22261 ccgctccgag gctcacatct actggggcga tacggcgggt gaccagatct cccgggccga
    22321 gaacgacgga acgggcgttg actccgactt cctctggtgg tcggtcggca acccgacggc
    22381 cgttgcgatc gacggcaccg ggatctactg ggcgaacaac accgacaact cggtccgtcg
    22441 ggcgaacatc gacggcagcg gagaaactct cagcgcctgg attccgaccg gcataaccag
    22501 cgcctggggc gtcgcgatcg attcgaccta cgcgtactac acgaacattg ccggcacttc
    22561 gatcgggcga aagaaccgga acaccggcgt cctcgaccag gccttcatca ccaacaccgg
    22621 attgcagccg gactcggtaa ccgtcagcgg gtcctacatc tactggagtc acagtggcaa
    22681 cgcgatcggt cgcgcaaaca tcgacggcac cggggccgac cccgatttca tcagcggcct
    22741 gagcggagtc cgggggatcg ccgtcaacgg gagctacatc tactgggccg gggataccac
    22801 gaacggaatc ggccgtgcca acctgaacgg gaccggcgtc aaccagagct tcatcaccgg
    22861 cgccgcgaca actgacggaa tcgcggttga ttccagccac atctactgga ccttgccggg
    22921 tccgggccgg atcgcccgcg ccaatctgga cgggtccggt gccaacacca gcttcatcca
    22981 gaacgccggc aaccccgtcg ccgtcgcggt tgacagcggg gtaagcccgc caaccctgac
    23041 gatcaattcc ggtccctcgg ggccgaccag caaccgcacg ccttccttcg gattcaccag
    23101 ctccggcgtg actctcacct gctcgattga tcagggcact cctgcgtgga ctccctgcac
    23161 ctcgccttat gccccgcccg agtccctggc cgacggtgac tggaccttcc gggtccacgc
    23221 ggtcagctcg ctgggcagtg aggcggacca gacccgcacg ttcacggttg acggacagcc
    23281 gccggaaacg atcctcgacg gtaccccgaa ccagttcacc aacgaatcca ccgcctactt
    23341 cgacttccat tcgaccgaac ccggttccta cttcgagtgc gacggcggcg atgacgactg
    23401 gcttctctgc agctctccgg ccacgatcgt ccaggtcagc ccgggagtca gtacgttcag
    23461 ggtccgcgcg accgacagcg ccggcaacgt cgacccgacc ccggcgactt tcgagttcga
    23521 gatggatcag atcaagccgg aaacgaccat cgactcaggt ccttccggaa cgatcaccac
    23581 aagccaggcg gacttcgcct tcagctcgaa caagtcgccg gcccagttct tctgcagcat
    23641 cgacggtgct gatgcggaag cctgcgcctc cacgaagaca tacacaggct tgtctgacgg
    23701 ccagcacgag ttcagggttt acgcccggga caagttcggc aaccaggact cgaccccgga
    23761 ctcccggacc ttcacggtcg acacctccgg tccggtcgat ccgccttcga acgccttcac
    23821 cttcggcagg ttgaagctga acactaagca gggcaccgcg accctccagg tcaaggtgcc
    23881 gggcgccggc aaggtgcagc tgctcggctc gaagactgtt gccgccagtt cgaagtcggc
    23941 ccgggcgaaa tcgaccgtca ccctggccgt caaggccagg ggcaaggcga cgaagaccct
    24001 caagaagagg ggcaaggtcg gcctcgcggt caaggtccgg ttcaccccga ccggtggcac
    24061 cgcgaagacc atgacgaaga ccgtgaagct ggtcaggaag aagaccggaa agtagcgccg
    24121 gtgacatccc cgggcatgag tacttcttga cttggagagt caggattatg cttgaatggg
    24181 aagcggtttt tcatggttgg gcacggtcga atttctctgg ctgcggtgat ctgcggcatc
    24241 gcgtttctgt tgtcaggcgc cattggcgag gcgggcttca tcccgaagct cgaccgcgtc
    24301 aaatttgacg acggcgaggt ctatcgtcag ggctgtatcg tcggcgacgg gcagctcagg
    24361 tccaggccct gccggttcgg ctaccggaac tcgaaacgga gggtggtcct gtttggtgat
    24421 tcgcacgccg ccgagtgggg tcctgccctg ctcaggctgg cccgggccga ccactggcag
    24481 gtgatcgtcc tgacccgggc cagctgcccg gccgccctga ccaacatcga ccgctactgc
    24541 aacacctggc ggcacaactc cctgaagcgg atcaggcgtt tgcgacccgg tctggtcgtg
    24601 gtcgcctcgg ccgcgaatta ccaccattac tggacccttc gcaagcgccg tgggctggac
    24661 cgccaggcga gcgaacgccc gctggtccac ggcatgatca ggaccttgag gtacctgaag
    24721 cgctggtcga gaaaggtcgt tctgatccgg gaccactcgg ccgcgccgtt cagcgtcacc
    24781 gactgcctgc gcaggaaccc gacccgcccg cagaggtgtg atttcaggtc aacccgcagg
    24841 aaggcctggt catacgaatt caaggcggcc ggaagggtgg acggtgtcgg agtgatcgat
    24901 ccgcagccga tgctatgccc cggccgccgg tgctacgcgg tcgacggccg ccacctggtc
    24961 taccgcaatt acggccacct ggcagcggcc tgggtccgga cccggaaccg gtggctcggc
    25021 cggaacctga acgatccctg gaacggaagg gacgtcaagc cggtcctttc gaacgattcg
    25081 tccgatgaac ggtcacggcc cgggtacctg gtccggtcga tcccctcccc gatgaacggc
    25141 caggcaacca gcgattgagc gacccggttc cggctggccg gccggggagc gctcagttgc
    25201 agccgtcggt ggcgaaccgc ttgaggcgcc gggacatctt cggatcggca ctcttcagct
    25261 ccggtgcgag ccgggtgatc agctcgcgca tcgactcgcc atccgattcc gccaggggtt
    25321 tctcgtggca gagaaccatc aacgcttcga gcttcgcctg atccccgggt ttcagcgatc
    25381 cgctcgcggt ggcatccgcc acggccgagt tggcggcgtt caccgtgtca gtctcgctgg
    25441 agtcgagctc cgcaactcct ccgcaaccgg cgatcaacag caccgcgcat gcggcaactc
    25501 ccaaagctct cctcacatca acctccggac gggatcaagg acgggcgaag cctacattcg
    25561 caagccgtcc agcgggactc ttgccggccg aaacccacat gaaaccgacg ctgtcgggct
    25621 atccttgacc cgtgggcgtt cagaccagca agactcctca caccgttcgc ctggaagagg
    25681 cgtctagaag agcgcatgag gcggaggcaa acggcgatga cgcggctgct atcgcggcct
    25741 ggcgcgagta tcgcctcgtt cgtgacgctg gccgtcccgc cgaggatttg cttgaggagg
    25801 gaatcactct gagcaagatg gccgatcggc tcatcgagcc gcggtgaccg actttggtcg
    25861 catactcaag gacttgaatg ctgcaggggt ccggtacgtg gtagttggcg gtatcgcgtt
    25921 gatccgtcac ggtgtggtgc gcgccacccg ggatgtggat gttgtctatg accctgaccc
    25981 gtccaatctg gaggcgattc gttcgttggc agagaaatgg ggtgcgaccc gacccgatgg
    26041 gtcacctctc ctggagaacg ccttctccgc tggccggtcg gttcatctgt cgacgccact
    26101 gggcgaaatc gacttgcttt cggaagagac ggcctcggtg agctttgtca acctgctgga
    26161 gagatccgag atccgccgca tcgacgctgt cgaagccccg atctgttcac ttgccgacct
    26221 ggttgccctg aagcgcgcag caggcagaga gcgcgatctc gcggatctct cggatctcga
    26281 agcaactcat gggagcttgc cagcccggcc cgaggactga agcacgcccg agcttcggct
    26341 tcgagattgg cagaatcaca gaagcatcct gccaggcccg caccaagagc gagatttgtg
    26401 gtgtggagcg gagggggagg gattcgaacc ctcgatacgc cgtaagacgc atgctggttt
    26461 tcaagaccag tgcattcaac cgctctgcca cccctccgtg gcctgttcgg cccggggatt
    26521 ctgacaggcc gcgggtcgat tggcgggatt gagccgccgc ggctagaaga cgaggaagag
    26581 cgcgccggcg acgaggagga cgacgaggac gatcaccgtc acctggatca tcggtcccga
    26641 catcaggaca tccggggagc cggtcgcgtc attgctgact ccgagctcct ccatctcttt
    26701 gcggatctct tcctcggatt cgacttcacc catcttgctc atgccgtaac tatcccacga
    26761 ccgcctgtca ggatggggtc gtgcgtcgtc tcctctttct ttcctgtgcg gtcgtcttcc
    26821 ttgacgtcac attcttctcg gtactgaccc cgctgcttcc cagctacaag agcgaccatc
    26881 acctcagcga aggggcgatc ggggtgctgg ccggtgcatt tgccgccggt tcgctggtca
    26941 tggcggttcc gggcggctgg ctggtggcca gggtgggagc gaggaagacc gtgatcgccg
    27001 gcctgaccgg gatcggaatc tgcagtccgc tcttcgggtt cgccgagcac atcgtgctgc
    27061 ttgatctgct ccgtttcctc cagggcggct gcggcgcgtt gatgtgggcg ggcgcgatca
    27121 gctgggtcgt cgttgcggcc ccgaccgcgc gccgggggga gctgctcggg atcgtgatcg
    27181 cggccgcggt ggtcggcgag ctgctcgggg cgccgcttgg ggcgatcgca catcaggtcg
    27241 gcaccgagcc ggtcttcagc atggtcctgg tcgcggccct gatcctgatc acgatcgccg
    27301 tgaccctgcc cgagccgacc ggtgtcgagt cccagcccct cggcgaggcc tggcgccgga
    27361 tccgccagtc cgacgtgccg agcggagtcc tgatgctggc cggaccttcg gtcgccttcg
    27421 ggctggtggt ggtgatcggc ccgctccgca tggatgatct cggagcgtcg ccgtttctga
    27481 tcgccgccgc cttcgcatcc ggctcggtcg tcgaagcggt ggtcggtccc gtgatcgggc
    27541 ggatctcgga ccgggtcggc cgcaccctgc cctacctgat cggggcgggt gccctgatcc
    27601 tcgccctggt cggcctcggg atattcgacc ggctttcact gctcttcgca gtggtgatgg
    27661 tgatgtcctt cggggccgga atggccttca ccccggccag cacgctggtc accgacacag
    27721 caaccgcggc cggggtcaac cagggttacg cctcgggagc atcgaacgtc gcctggggcg
    27781 gcggccagat gatcggcgcg atcggcggcg gcgcattggc cggtgcggcc ggataccttc
    27841 tgccctgtct ggtcgccgcg gccgtgatgg ccggggtggg aatcgtggcc cgttcactcg
    27901 gcatcggacc gccggtgatt tcggacctga gcgaggaagc aggtacagcg gatggtcggt
    27961 gagaactatg acgcccggaa gctcggaagc ggcgggcacg cctggaccaa ctgggccggt
    28021 catcagagct gccggccgcg cgacctgatc cgggtcagcg gggtcgggga gcttgccgag
    28081 gtcgtcggtg cggcgggcgc ggccggccgc cagatcacca cggtcggctc cggacattcg
    28141 ttcacgccga ccgcgctttc ggatgacctg ctgctcgaca tctcccggat ctccggggtg
    28201 atctccgccg accgggattc cggcctggtc gaggtcgggg cggggacggt tctcgccgac
    28261 ctcaaccgtg aactcgatca cctcggtctg gcaatgccga acctcgggga catcgatcgc
    28321 cagacgatcg ccggcgccgt ttccaccggg actcacggaa ccggccgtgc cctgccgaac
    28381 atctccgcac agatcgagaa ggcccgcatc atgggagccg acggggagat ccgcgagctg
    28441 gaaccgggct ccgacgggtt gctggccgcc cgggtcgcgg tcgggtcgct cggtgtgctg
    28501 gtctcggtga ccctgcgtac cgtccccgcc ttcaacctgc atcgggtcga tgcgccgatg
    28561 gacctcgacc aggtgctcgc cggttttgac cggatcacgg aagaaaacga gcacttcgag
    28621 ttcttcgtct tcccctacac ccggaccgcc ctgaccctga tccgcaaccg caccgaccag
    28681 ccgctcgccc cccgctcgca ggccgatcgc ttcctcggtg acgtggtggt cgagaacgga
    28741 ctcggtgacc tcgccctccg cctgaccggg cggttccggt cggccatccc gaagatggcc
    28801 cggttctcga ccggcttcat gtcccagtcg gagcagatcg atgtttcaca ccgggtcttt
    28861 gcgaactacc ggacgatccg cttcaacgag atggaatacg cgctcccggc cgagcatggg
    28921 ccggaggctc tcgaccgggt actggagctg atcgaaaccg aaaacttccc gctggcaatg
    28981 ccgatcgagt gccggatctc ggccggtgac gacgccctga tcagccaggc ccacgaacgg
    29041 ccgaccgtct acatcgcggt ccatcagcac cggaccatgg aatacgagcc ctacttccgg
    29101 gagatcgaac ggatcttccg cgagtacgac ggccggccgc actggggcaa gc
//
LOCUS       DAOQKN010000007        27864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_689_length_27864_cov_6.080046, whole genome shotgun sequence.
ACCESSION   DAOQKN010000007 DAOQKN010000000
VERSION     DAOQKN010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 27864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27864
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_689_length_27864_cov_6.080046"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="PLJ64_01035"
     CDS             <1..684
                     /locus_tag="PLJ64_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNC92107.1"
                     /translation="RFEGERERTWRTLRAVKNRFGATSEVGVFEMRSGGLTEVLDPSA
                     RFVEEATGAQGSVVLASMEGTRPVLVEVQALVSPSELEIPRRVVNGIDRNRLSMILAV
                     LARHARLGLGTADVFVNVVGGVRIDEPGADLAVALAVASAQRGETLSGPDGRPLACFG
                     ELGLTGDLRYVAHADRRVAEAAKFGLEPVIGPRGGERLSGLAPCATVKQALKAAFSGS
                     SADSRAAAA"
     gene            808..1920
                     /gene="disA"
                     /locus_tag="PLJ64_01040"
     CDS             808..1920
                     /gene="disA"
                     /locus_tag="PLJ64_01040"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HNC92108.1"
                     /translation="MSPAVESDLAHLSERQDPRLLRAIDAVAPGTELREGIDNILHAR
                     TGGLIVVGETEDFAFMLSGGIKLDIDYTPAMLYQVAKMDGAILVNADGSKITWANVQM
                     MPDPTIHSSETGTRHRTAERISKQVDALVIAISERREVVSLYLEGVKYILQDIPAVLA
                     KSNQALATLTKYRTRLDELSSRLTRAEFVGGAVLYDALMVLQRSELVSRMATEVERYV
                     IELGTEGRLIEMQLEETMVGVASERLALMRDYAVEDTEENVQFMLEELAALPHQDVLD
                     FGRLAELLGYDRKVNTLDLATEPRGYRMLGHVPRLPRLVVQKIVMNFGSVEAALAASD
                     EELAAVDGVGPARAKEIREGVRRLQESVMADRFLNT"
     gene            1980..>2285
                     /locus_tag="PLJ64_01045"
     CDS             1980..>2285
                     /locus_tag="PLJ64_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HNC92109.1"
                     /translation="MNGEEVTDEDLDLTPYVCDFEEGDKVVYPHHGAGVVLKKADAEL
                     MGETREYLTIKILHNDLTVKVPTAKAGEAGLRRVIDEDEIKKVISVLSDEVSDMPKNW
                     "
     assembly_gap    2286..2295
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2296..2555
                     /locus_tag="PLJ64_01050"
     CDS             <2296..2555
                     /locus_tag="PLJ64_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HNC92110.1"
                     /translation="RRFKYNKEKIKTGDVFELAEVVRNLALREMEKGLSTGEKQMYTR
                     AKKILASEFMYALDKDEEGAESYLDGLLEERYEASVAAAAA"
     gene            2581..3252
                     /gene="ispD"
                     /locus_tag="PLJ64_01055"
     CDS             2581..3252
                     /gene="ispD"
                     /locus_tag="PLJ64_01055"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNC92111.1"
                     /translation="MGVPALIVAAGSGQRLGAGGPKALVELSGRPLYAWSVDAFRRAT
                     RIDRIFVAVPPGQAGQFEEPGIITVEGGETRSHSVANGLAAITADGDPGMVVVHDAAR
                     PLVSPALIDAAVIGLDSDPELHALIAAAPMTDTVKRLGEDGIVVETLDRSELVSVQTP
                     QVFRIPNLQAAIAAGDLSAATDDASLVEAVGGSVGVIESPSGNIKVTVPADLELAALL
                     LERSR"
     gene            3249..4094
                     /locus_tag="PLJ64_01060"
     CDS             3249..4094
                     /locus_tag="PLJ64_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HNC92112.1"
                     /translation="MTDPVITDYHLHLRGDELSARAEDHFTTENIARYLEAAGQVGVT
                     DLGCAEHMYRFTEALTVWRHPFWEEWAIDDIDQYVEAVRDSPIKLGIEGDFIVGAEEK
                     LESMLGSRPFDYVVGSVHFLGDYSLDTEDYTIWDGSQDPDEIWRRYFETLASAARSGL
                     FDILAHPDLVKVWGRAGRLPSVDPRTMYEPAIEAIAETGIAVEVSTAGLRKGVEEIYP
                     APAFMEMCVEAGAVFALSSDAHEPDQIGFRYGEGIDFLNEHGITEIATFRDRVRTTAP
                     LGNPK"
     gene            4152..>6987
                     /locus_tag="PLJ64_01065"
     CDS             4152..>6987
                     /locus_tag="PLJ64_01065"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92113.1"
                     /translation="MTTGNQKTRGILIALALLLGALMMFGPARAEAAGYPNISMSKTT
                     SGSVLLGGNSNVTLTASNPNGQPTGYNLSFRDVLPPGISYVPGSSPSSTGEPQVIANA
                     PAAGQTTLIWSNVADLTPNSTFTFTFQVTNSPAVFDIGDTYQNTAGSYLNCDPRYVPK
                     FNAQGQPTQSGGNATCSGLPQETSYTGSATASANTLIKAIEIDKSEPSPEGQLLRGLH
                     DHQTVYTLTVTNNEVDADDNVKVEDYLPAGLEFLGCGTQDNTTDAPTNPGSAEEYPGS
                     GAINPGNAPAAPDCVVPDTVETVLNPSGLPAGVYTHVVWNNLGNLGPSGQLRIQYVAA
                     VPIRQNTMTWTNGVPPVTGTQGSNLDNNSGPETTHLQGLTNHAKVTSDHQGPGGPTEV
                     TDSEDHTVQAVDIRIVKSVTPAGLAVGQISTWSLAIATSEYRYFEDVEVSDTLGDGYC
                     PLGPVNYEHTPPPATAECNPTGNNPSVPYTSAVEQTNGTWQITWDSSTAPALGTINPN
                     SNLTISFPTKTREYFQQNYVNSTPVLSNDTGRNDVDIAGAAWVICAPGAPSPCPNLSP
                     DKIDTDFTDGTVLDDDSDATQVGSGPSINKQVSPTVNGNCGSLNDYASGITPVASPGD
                     TICWRLTMSFPNNLTTSNVFVGDYIPPGTTYVFNSTVALPSNTVGIFTGSPPQPDLFG
                     TSGLRWALNDGGGEVQPGKVFDVVFATKVQRSTDSVDGDIEGNLMKAVYTNSDGTSFP
                     LRDEEDFERSQPEISLLKGVYKVNGQPPAGNPPNVDGAVVDGGDDVTYRVDVTNDGSV
                     NAINTQVWDILPPQVTCADVDAGSISNGGTCNAGQNRIEWSGLTVPATGNLTLTYTVE
                     IPDGLSGGDVLNNTAGVREFASSSGSGPYVQVPENNIDPTLDPNANAPAAKDPSNVVI
                     ESSTTVKTRVTDINQPGNSPTTQATIGERIN"
     assembly_gap    6988..6997
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6998..10988
                     /locus_tag="PLJ64_01070"
     CDS             <6998..10988
                     /locus_tag="PLJ64_01070"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HNC92114.1"
                     /translation="VTATIPQGTRLYGPTALVDNVSSRMTVVPLSASATLDNGTGGGQ
                     VPLPTAGLTLTQTGNVVKVDFPNPYSNPEHSGDDVIVLTFGAIVRDVYPTNYAQGNST
                     QYTMPNTGTLSWKDTNGTNRSASGSVNTTIVEPRISIDKTVDDDGPVSPNDLLNYTIQ
                     VSNGSAARVSTANNTVLTDKVPVGLTPANGGVPVADGGTVNPDGGIWNLATRTITWPT
                     IPTINPGAITNITYQARVDNPAVGSDLLINHAQAATTSMPGTPTVERDSSTAAPGYLV
                     SDEVPVEVSSPAIVKTGLPAQATIGQHVTVTVFASIAANLTNYDGTVEDDLPNGLIFD
                     NYVSEECIEGCSGPVTISTLGVVPNGTGQRIGWWIGDVPAAPDSRVVALTYRAHVADQ
                     YQVPPGGPVLDGDALVNTATLLYNLTDKVTVPPTSPPDPSTFDRSLDDDHTTTVVEPD
                     LALDKSVSGDDDQDDSRTTQPGDSYTFSIKVTNNGTSPAYDLTVTDQPDPALTNIQVN
                     HSPVFEVPVLPVNSNDPIQWFIPGPIDPGESVTLTYTADLVPSAQLHNMQQVVNTAEL
                     PSYWGVSAGEREDPDNAGIDYREYEGNNDTVTMTVLVPTVTLDKTTGLPQFPDTGDAQ
                     TETPFPWRILITNPNTGSSLLGVDLTDTLPPNWTYVPNSAQITGTGTLTPGGQVNPTI
                     TQNPTGDVLVWSNIANLVGVQNAVVSFNAIPEPEAAIDPGLTNPHVNEAEAIGEDTSG
                     ATASGDGPYTDDDDATATLETPVTDIQIVKTADDPAPTAGTNTTWTLVVKNNGPKVSP
                     DVEVSDVLPAGLTYVSATSSQGTCAENPAGTMHCQIGQMNVDDEVTIHLTTAVGAGTV
                     GQTLTNPADVDDPYIDDSNPANNHSEDDVIPTESADVGIGKSIDEPLFAGQTGSYKLT
                     VTNHGPSVARGVTVTDTLPENLTYAGATTPVGSCSGSGQDFSCDLGDLDPGQTVVIDV
                     SVNVLEGGEYTNCADVASPTPDPDGDNNHSCTTNPAENTDLAIEKTGPPYFPEGKNRN
                     YTLLVTNVGDQPTGGTTTVTDRIPDVLRPEAAYGNGWSCAISGQKVTCTRSDVLEPGD
                     SFPLITIRVVARENLLFRPIGNTGHVHLPGDPNPDNDQDTVRTKKGAICEGGSLALNP
                     AFVWVGQKTIVKATLRTRAGAPAIGLPVRIKGNGKGGASGKLRILTTNGKGKVHFTAR
                     ATSTEARWTVSVPQSHQKAVLAPKRQETCRAMNVTPRTIDINKTTVLTVRLRTPEGRP
                     AIGITVVARGQGTGDSARTNGKGMARLRVRPNGQGYLVVTAPNATRCRFRIGASDLDA
                     AGAGNQLTG"
     gene            11018..11713
                     /locus_tag="PLJ64_01075"
     CDS             11018..11713
                     /locus_tag="PLJ64_01075"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNC92115.1"
                     /translation="MLVSLSLLLVWDGSAAASAARPEAFAEKVALPAVGEALAPRIAV
                     RARPISKAPVRAVMKAFRFDYRPTVFFAIDRRETGQGEWLKVRIPGRPNGRTGWVRGS
                     RIRLLRSAGPFRIVIDRSQRRLRLYRHKRMVMKTKVAVGTPDAPTPLGHFYLTAGFVP
                     GDDFLGPWAFETSAYAAITDWPRGGIVGLHGTSETWSVGLRASHGCLRVYNKVILALK
                     KRVRPGTPLRIRA"
     gene            11754..12215
                     /gene="ispF"
                     /locus_tag="PLJ64_01080"
     CDS             11754..12215
                     /gene="ispF"
                     /locus_tag="PLJ64_01080"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNC92116.1"
                     /translation="MAISVGIGYDSHRFADDRRLILGGVEVPHEMGLSGHSDADVLTH
                     AIIDAVLGAAGLGDIGEVFPDTDPAWEGADSISLLSTVIGMLRGPITNVDATLICEQP
                     KLGPHRPAMVARLSEITGSRVSVKATTNEQMGWIGRGEGIACMAVAAVNLD"
     gene            12239..13858
                     /locus_tag="PLJ64_01085"
     CDS             12239..13858
                     /locus_tag="PLJ64_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNC92117.1"
                     /translation="MNPVKSAAQAVANKAFEIRTLHKAGVVGGLGPAALPVAASALIR
                     GGTTPATAIRVAAVKNGDRVYLEDERGSLTFAEVHRRSNALAHAFAGLGIGPGDGLAV
                     MCRNHRGFVETNLAGWKLGANVILLNTMFSGPQLSDVIEREGAKAVVLDQEFEELLSG
                     VPGGIGRVCGFEDEPGTSGLPSLEDLIAGNDDGELPWPSEKGRNVILTSGTTGTPKGA
                     QRPAPSGLSVMVGMLDRIPHFSGETIVMAAPLFHSWGYLNSIIGTSVGARLVLQRRFN
                     PARTMELVDEYDADALVAVPVMLQRILELDAETSKHFKASKLGIVTLSGSALPGGLAT
                     AWMDRYGDNIYNFYGSTEVAMGSIATPEDLRHDPATAGRPPYGTSVRLVDEFGDEVPH
                     GETGRIFIRNEMTFDGYTGGGNKENLDGYLSSGDVGHFDEDGRLYIDGRDDEMIISGG
                     ENVYPAEVEDLISGHDQVVESAVIGVDDEKFGQALRAYVVLKTPDSLDEDGVRKYVKA
                     NLASYKAPRDVIFLPELPRNATGKILKKDLA"
     gene            13904..15064
                     /locus_tag="PLJ64_01090"
     CDS             13904..15064
                     /locus_tag="PLJ64_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92118.1"
                     /translation="MSDTTSAPSAEEAGSGNGLPPELRQVGEASAKLNSRIETLAKEL
                     SASESKAREAEIASREAELKALATARDSLTEARKAKAEAERDRAAAATADQRVEAVKA
                     QATRDVAEAREEIKTKARETLRKLSAEAREKIEASRKARLEAEEKAKELEQKDADQRE
                     AQARVEADLKRVEGTLKQERETRDEAMAKVAAEGEERLAARTRHLEEVAERLAKEARQ
                     AAEREAKNRIEAADERTRKAQERAAQAEAHANEFESLARQRIAEIEADVDRRVMEGTE
                     QVRREAAERVEELIERFGREAEENARRRADERLREEDERIQREADRRAELARQSAEDD
                     VQAAADRARQEALRAASSTAPEWERGDSGEGDGGPAPRRRRGGPGAGGYRTF"
     gene            15082..16455
                     /gene="cysS"
                     /locus_tag="PLJ64_01095"
     CDS             15082..16455
                     /gene="cysS"
                     /locus_tag="PLJ64_01095"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNC92119.1"
                     /translation="MREVRIRDTMSGELLTLGTDEEIGIYACGPTVYSRIHIGNARPF
                     VIFSLLARFLRSEGYGAKLAINVTDVNDKIYTAAAGRGVSSTELAAEMTAAYFEDTGR
                     LGIGRPDSEPLATETIGPIIDLIATLVEEGHAYESGGDVYFAVRSFDGYGKLSHRPTE
                     EMEQGEGDDAGSLKRDPLDFALWKAKKDGEDLSWESPWGPGRPGWHIECSAMAEAELG
                     QPFTIHGGGSDLVFPHHENEIAQSEAARGEQLARIWMHNGMIETGSEKMSKSVGNIFQ
                     LSEALDAYGAETVVAYLISGHYRQPLAFGEGPLEQAAAGNERLRNFLRENPPAGAGEG
                     VPGDEAAAAGSRFRDALADDFNTPRAMREAFDLVRAVNQRPDDPALAGAGTVLAGMLE
                     LVGLESLATGGEEGPGDEALELLEARNAARADKDWARADEIRDSLAELGWEVRDGAEG
                     ARLVPRS"
     gene            16455..17117
                     /gene="rlmB"
                     /locus_tag="PLJ64_01100"
     CDS             16455..17117
                     /gene="rlmB"
                     /locus_tag="PLJ64_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNC92120.1"
                     /translation="MSDPEIIYGRNPVEEAKRGRRKVLRVWEAPETPETRLVELCGST
                     DHQGIVAEVEPFPYLSGSDLLAGEDALVVVLDQIQDPRNLGAICRSAEAAGATGVVIP
                     DRRAASVTAAAAKASAGAVEHLKIARVRNISDWIIEAKDAGFWIWGADGRADQAPWQV
                     DLTGKTALVLGTEEKGLRQRVADSCDGLIAIPQSGQVDSLNVSVAAAVLIFEAIRQRS
                     RT"
     gene            17403..18101
                     /gene="sigH"
                     /locus_tag="PLJ64_01105"
     CDS             17403..18101
                     /gene="sigH"
                     /locus_tag="PLJ64_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HNC92121.1"
                     /translation="MATPTSISHNHAVSDVPARSRGSVSNPRLEPDDETLVAFAKRGK
                     TDAADRLIRRYHGFVRLKASSYFLVGGDSDDLIQEGLLGLHKAIRDYRTDRESSFRNF
                     AELCITRQIITAVKTATRNKHVPLNQYVSFGQSPASNTESETTMEEILPGPTALDPVN
                     QAISSEELSSLVSCLQSTLSEMESSVLGYYLEDRSYEDVAALLGCETKTVDNALQRVK
                     RKVSKHLESRDIAV"
     gene            18196..18269
                     /locus_tag="PLJ64_01110"
     tRNA            18196..18269
                     /locus_tag="PLJ64_01110"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18230..18232,aa:Thr,seq:tgt)
     gene            18315..19169
                     /locus_tag="PLJ64_01115"
     CDS             18315..19169
                     /locus_tag="PLJ64_01115"
                     /inference="COORDINATES: protein motif:HMM:NF015921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92122.1"
                     /translation="MNSLAQRLPTTGADRSLARQMLNKVPEVTLYFWIIKVLCTTVGE
                     TAADYMNVDLGFGLTNTTYAIGALLVVALIFQFRSRRYVPGIYWTAVVLISVVGTLIT
                     DNLVENMNVALQTTTIGFSVALAITFVVWYAFERTLSIHSITTTRREAFYWLAILFTF
                     ALGTSAGDQIAEQYGLGYFVSILIFAAAIGLVALAHFRFRLGAVTAFWVAYILTRPLG
                     ASIGDWMSQPTANGGLGLGTTVTSFIFLAAILGLVIYLSVTKVDQTPPEVVEAEMEET
                     EEAEAVSV"
     gene            complement(19182..20528)
                     /locus_tag="PLJ64_01120"
     CDS             complement(19182..20528)
                     /locus_tag="PLJ64_01120"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNC92123.1"
                     /translation="MKGTIRQRLTFSVVCVTAVMLALLVVGFNLSLRASLDGDVDRLL
                     HARAQATLDNIDLENGKLEVSEGSDDGAEDALVWIYDDRKPLESPQVASQLDRVAASL
                     AAEGSGERDDSDSDTRLFARPVVQNGRVVGTVVTGVSLEPYERTADRAAFASVVLALI
                     MLVLITITTRLVVGRALKPVSEMTEEAAKWSEHDLDRRFNEGEPTDELTRLAATFDTM
                     LDRMAYMVRHERNFSAELSHELRTPLSAISAEAEIALTKDRDAAEYRESLERIAERST
                     ELTKILETLLDVARSEGSGGSDEAADLLDSVDSAVAAAGSLTDRYGVVVELDRPEGRI
                     RAQVAGDTFQRMISPIIENAITYARATVSIAIRSRGRTAEVVIRDDGPGFSPRDLEAA
                     FEPGMRGIAARNEAAPPGTGLGLALARRLARANGGDVSIPVSGDGGTVILTVPIAS"
     gene            complement(20525..21190)
                     /locus_tag="PLJ64_01125"
     CDS             complement(20525..21190)
                     /locus_tag="PLJ64_01125"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC92124.1"
                     /translation="MNLNVLVVEDNPELRRLLNQGLDEEGFEVDLARNGREALMLADP
                     QKHDLLVIDIGLPDSDGRDVCQALKSKGIEAPVLFLTAKDSGPDRVSGFSAGGDDYLT
                     KPFVFAELVARLRVLGRRLTRGDRSTSGDLRLDPVRHVLTCGDRETELTPTEFRLTGA
                     MIARSGEVIRRREMIAAAWPPGAIVHDNTVDVYVRRLRRKLDELESESRIHTVHGVGY
                     RLE"
     gene            21350..21832
                     /locus_tag="PLJ64_01130"
     CDS             21350..21832
                     /locus_tag="PLJ64_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92125.1"
                     /translation="MTSKIKKILASLLALAALALGGAAIASATNGGSDDDHGAQEKAE
                     VNDHDSDANENESTSEGADDNEAEDGQEAADPGEAVSSTVAAKAEAAAVARTGGTVQE
                     VTAETNDAPEKGDDQDPGEKAAPAGTAYEVDLTKGSQELKVSLDRQFNVLQVQTGQDD
                     "
     gene            complement(21937..22557)
                     /locus_tag="PLJ64_01135"
     CDS             complement(21937..22557)
                     /locus_tag="PLJ64_01135"
                     /inference="COORDINATES: protein motif:HMM:NF037470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QsdR family transcriptional regulator"
                     /protein_id="HNC92126.1"
                     /translation="MEAATTSTSKAFAAHQDEAAGLGAPRATPADAFRAALRAFVRGP
                     RLDMEKLAQDLGISKTTLYRWTGPRDKLLSEILCFFGEEAARIGREASEGATGVRRIE
                     LFTRGFVESIVNFEPHRRFVRSETELAFRLVLTRGSQVQSTVVERFAEVLREEEASGN
                     LGLRAPAGDLAYAAIRIIEGFIYNDALTEDSPDVEKAVSIVCLLVD"
     gene            22687..24630
                     /locus_tag="PLJ64_01140"
     CDS             22687..24630
                     /locus_tag="PLJ64_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92127.1"
                     /translation="MKSKLIAAGAVLVAVLLTGVLGAGAASASSAKPPFYPIDKKTIK
                     VPNDVQPWLPTYSPDGKHIVFQNQLDGSTWATSKDGKGTSCITCEFSDRPDISGGFTY
                     VFPDNKRLFVSHELGGLTGVDSGPNTDAWILECSPSIYDCQSHQYLPVDMSDDKVGVP
                     LIVQRRTWHLAPDGVHLGWMDIRLDGTVMIVGRLTRLDDRYKVVDQKAVNPYRPTSLT
                     DTDAANWAGDNQLWELKSFADGGASILALGGAGYNIDPVKINLKTGKVTRLTANPDWD
                     EDGAISPDGKMLALYSWRTRHRLDAFGWIPQIDSFVEMPAGAALAPFYVSTWPGFQCD
                     LSPWLLSSSGDMGGKLIGQPLNVYPENNLTPGNNLSGNQFWSPNSQDVLLQERTRTIP
                     ATTLSEHVQQKGLTPNRIAIAHIERPAGKKLKVVSSEVGNWAVAPTQYFPSVGSNTSV
                     TVNGKAGGTADLVYTGNLQTGSWSSEYHDYTKDGKTFVNGTYFVSRTIEGVWTLKADV
                     TVTGQHTGRLNMDMTIQGGDPLPIMTGGITAVYDGKKAPALPKLGACYKKLPQKSRLA
                     AQVKSKRAGKATKVTVTVTANVYGDRRPVQNAKVKLAGRIVRTDRSGKAKLKVRGRGT
                     RKVLVTAGDTFIKTSKVAHLKQR"
     gene            24732..25892
                     /locus_tag="PLJ64_01145"
     CDS             24732..25892
                     /locus_tag="PLJ64_01145"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC92128.1"
                     /translation="MPVGYLITLFLLGTCVLLLLAPVRKRPLGLVSFWGGLLVNEQAH
                     WFAVLLAGSTVLAFVEGDISGVARAIAGLAVLAEAGLIVLFLRSFRSRGTLREALAAF
                     DSDSPAALAGRRSYLAALAAPPAMGRSRVRLKSDIPYGPVPGRANRLDLYLPRHGKVT
                     GPTMIYMHGGRFRSGNKRREAQAIKCQLARRGWSVISANYRLNPEGSFPDYLIDLKRV
                     IAWARGEGQAFGIDPERIFLAGGSAGAHIAAMAGLTAGKPELQPGFEQVNTAVSGVIG
                     IYGYYGGLESPGGRPPAVPSSPRRHVQPGAPPFLLIHGDHDSVVGRGGAAALVKELQG
                     AGNQASLAVLPGAEHTFDLLRSVRTENTIDSIVDFAAWAERSENRDLSQSRR"
     gene            25904..26662
                     /locus_tag="PLJ64_01150"
     CDS             25904..26662
                     /locus_tag="PLJ64_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92129.1"
                     /translation="MGAKGAKGNLPGGLRTGLVGLGLLALAITCLLTSPAPDRSNAET
                     RANAEPQGGILPCGSHVVLTAVYRNRDGRIHFEGVADPKFAGRQARIYDRDNHLVAAT
                     TVGEDGIFWANANSKDEGYTWLTRFIARVGGSESRWKRIGQAVGLRDRQPFELEPTAR
                     NHGKTLERTRVRVKVAGGHSNLLVVGLQVGCSRFDVIELASTYTDSNGVSDLNLPRPP
                     ADRPYWIFRVSTEDDWKISPPIVVKSVPESGPTG"
     gene            26735..>27864
                     /locus_tag="PLJ64_01155"
     CDS             26735..>27864
                     /locus_tag="PLJ64_01155"
                     /inference="COORDINATES: protein motif:HMM:NF013383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNC92130.1"
                     /translation="MTQEQLEAYHEYTRKHGVNRGLYLFARIVLQPFFLIYLRLSRQG
                     RGNAQIKGATIVAANHRSFLDPFVIGTCLPWNKPMNYVAKVELFEKRWQGFLLCRVGA
                     FPIRRGEADELAIETAELVLARGGTICIFPEGTRIRKGSLGKPKRGVGRLALRSGAPV
                     LPVAVTGSENVRRGWQIRPRKVDIRVGRPMSFPQSESPSPALAASVTDRIWPNVELQW
                     EDIGGLPPMRRAAVIGAGSWGTAVAVLLARGGLEVQLGTRTADQAEEMALARENTRYL
                     PGVRLPDGIEVRASSQIELGGVDLLCIAVPSASYPQAVGAVADRVGRRAGGLLLAKGL
                     IAPKGQLPSEYVGERIRSRGVSCLGGPAHAKEAVSGSAALVLG"
BASE COUNT         5183 a         9366 c         8859 g         4436 t
ORIGIN      
        1 cgcttcgagg gcgaacggga acgtacatgg cgcaccctgc gggcggttaa gaacaggttc
       61 ggggcgacct cggaggtcgg ggtgttcgag atgcgctcgg gaggcctgac cgaagtgctg
      121 gatccgtcgg cccgcttcgt cgaggaagcg accggggccc aggggtcggt ggttctggcc
      181 tcgatggaag ggaccagacc ggtgctggtc gaggtgcagg ctctggtctc cccgagtgaa
      241 ctcgagattc cgcgaagggt cgtcaacggc attgaccgca accgcctcag catgatcctt
      301 gcggtgctgg cccggcacgc gaggctgggt ctcggtacgg ccgacgtctt tgtgaacgtg
      361 gtcggcgggg tgaggatcga tgagcccggg gccgatctcg ccgtggcgct cgcggtcgct
      421 tcggcccagc gcggcgagac cctttccggg cctgatggcc gtccgctcgc ctgtttcggt
      481 gaactgggcc tgaccgggga cctgaggtac gtcgcccatg ccgaccgcag agtcgccgag
      541 gcagcgaaat tcggcctcga accggtgatc ggcccaaggg gtggagagcg gctatcgggg
      601 ctcgctccct gcgccaccgt gaagcaggcc ctgaaagccg cattttccgg aagttcagcc
      661 gattcccggg ccgccgcagc ctgatcttca ggcgaggcta aattcggctt tccggagcgg
      721 attgagggcc ggtcacaacc cccgcaaaag agcgcccgga accacgccag accgcattag
      781 aaagccgaat ctggcataat tctctgaatg tcaccagcgg tagagagtga tctggcacat
      841 ctgtctgaac gtcaggatcc cagactgctg cgcgcgattg acgccgtcgc ccccggcacc
      901 gagctgaggg aagggatcga caacatcctc cacgcccgta ccggcggtct gatcgtggtc
      961 ggggaaaccg aggacttcgc cttcatgctt tccggtggca tcaagctgga catcgactac
     1021 acgccggcga tgctctacca ggtggcgaag atggacgggg cgatcctggt caacgccgac
     1081 ggcagcaaga tcacctgggc caacgtccag atgatgcccg acccgaccat ccactcctcg
     1141 gagaccggca cccgccatcg caccgccgaa cggatctcga agcaggtcga tgccctggtc
     1201 atcgcgatct cggaacggcg ggaagtggtc tcgctctatc tcgaaggggt caagtacatc
     1261 cttcaggaca ttcccgcggt gctggccaag tccaatcagg ccctcgccac gctgaccaag
     1321 taccggaccc ggctcgacga gctctcgagc cgcctgaccc gggccgaatt cgtcggcggc
     1381 gccgtcctct acgacgcact gatggtgctg cagcgctcgg agctggtttc ccggatggcg
     1441 accgaggtcg agcgatacgt gatcgagctc ggcaccgaag gcaggctgat cgaaatgcag
     1501 ctcgaggaga ccatggtcgg ggttgcttcg gagcggctcg cactgatgcg cgattacgcg
     1561 gttgaggaca ccgaggagaa cgtccagttc atgctcgagg aactggccgc cctgccgcat
     1621 caggacgtgc tcgacttcgg ccgcctggcc gaactgctcg gctatgaccg caaggtcaac
     1681 acgcttgacc tggccaccga accgcgcggc tacaggatgc tcggccacgt tccgcgcctg
     1741 ccgcggctcg tggtccagaa gatcgtcatg aactttggca gcgtcgaagc cgcgctcgcg
     1801 gccagcgacg aggaacttgc cgccgtcgac ggcgttggcc cggcccgggc caaggagatc
     1861 cgcgaaggag tgcggaggct tcaggagtcc gtgatggcag accggttttt gaacacatga
     1921 cgacgcattc agtcgtcagc taagagacaa aggagcatct gatttccgat tcagaggcag
     1981 tgaacggcga agaagtgacc gatgaagatc tcgatctgac tccgtacgtg tgcgatttcg
     2041 aggaaggcga caaggtcgtc tatccgcacc acggagcagg cgttgttctg aagaaggccg
     2101 atgccgaact catgggcgag acccgcgagt acctgacgat caagatcctg cacaacgacc
     2161 tgaccgtcaa ggtcccgacc gcgaaggccg gcgaagccgg gctgcgccgg gtgatcgacg
     2221 aggacgaaat caagaaggtc atttcggtgc tgtcggacga ggtttccgac atgccgaaga
     2281 actggnnnnn nnnnnaccgc cggttcaagt acaacaagga aaagatcaag acgggtgacg
     2341 tcttcgagct ggccgaggtg gtacgcaacc tcgcgcttcg cgagatggag aagggcctct
     2401 cgaccggtga gaagcagatg tacacccggg ccaagaagat cctcgcctcc gagttcatgt
     2461 acgcactcga caaggacgaa gagggagccg aaagctacct cgacggcctg ctcgaagagc
     2521 gctacgaagc ctcggtggca gccgccgcgg cctaggccgc cggcctgaac ggtccccgag
     2581 atgggggtgc cggcattgat cgtggccgcc ggctccgggc aaaggctcgg agccggcggg
     2641 cccaaagccc tggtcgagct gtccggccgt ccgctctatg cgtggtcggt cgacgcattt
     2701 cgccgggcga cccgcatcga ccggatcttc gttgcggtcc cgcccggtca ggcgggccag
     2761 ttcgaggaac cgggaatcat cacggtggag gggggcgaga cccgttccca ctcggttgcc
     2821 aacggtctgg cggcgatcac cgcggacggt gatcccggca tggtggtcgt tcacgatgcc
     2881 gcccgcccgc tggtcagtcc ggccctgatc gacgccgccg tgatcggcct ggactccgac
     2941 cccgagctcc acgcgctgat cgccgcggct ccgatgaccg acacggtcaa acgcctcggc
     3001 gaggacggca tcgtggtcga gaccctcgat cgttccgaac tggtctcggt gcagacgcca
     3061 caggtcttcc ggatcccgaa cctccaggcg gcaatcgcgg ccggggacct ttccgcggcg
     3121 accgacgacg cctcgctggt cgaggcggtc ggggggtcgg tgggcgtgat cgaatcgcct
     3181 tcaggcaaca tcaaggtcac ggtccccgcg gaccttgaac tcgccgctct cctcctcgaa
     3241 agaagccgat gaccgaccca gtgataaccg actatcacct ccacctgcgc ggtgacgaac
     3301 tctcggcccg ggcggaagat cacttcacga ccgaaaacat tgcgcgctac ctcgaagcgg
     3361 cggggcaggt cggagtcacc gacctcggct gtgccgagca catgtaccgc ttcaccgagg
     3421 ccttgaccgt ctggcggcat cccttctggg aggaatgggc gatcgatgac atcgaccagt
     3481 acgtcgaggc ggtcagggac agcccgatca aactcgggat cgagggtgat ttcatcgtcg
     3541 gggcggagga gaagctcgag tcgatgctcg gctcgcgccc cttcgactac gtggtcggat
     3601 cggttcactt cctcggggac tactcgcttg acaccgagga ctacacgatc tgggacggca
     3661 gccaggatcc cgatgaaatc tggcgccgct acttcgagac cctcgccagc gcggcccgct
     3721 ccgggctctt cgacatcctg gcccatcccg acctggttaa ggtctggggc cgggccggac
     3781 gccttccctc ggtcgatccg aggaccatgt acgagccggc gatcgaagcg atcgcggaga
     3841 ccggaatcgc cgtcgaggta tcgaccgcgg gcctgcgcaa gggggtggag gagatctacc
     3901 ctgccccggc gttcatggag atgtgcgtcg aggcgggggc cgtcttcgcg ctttcgtcgg
     3961 acgcccatga gccggaccag atcggcttcc ggtacgggga agggatcgac ttcctgaacg
     4021 aacacgggat caccgagatc gcgactttcc gcgaccgggt tcggacgacg gcaccgctgg
     4081 gtaaccctaa atagaggcgt tcaggaaacg gattgttcag aaaaacagat cagggacgga
     4141 acactggcga aatgacgacg gggaatcaga agacacgggg aatactgatc gcgctggctc
     4201 tcctgctcgg cgccctgatg atgttcggtc cggcccgggc cgaggccgcc ggctacccga
     4261 acatctcgat gtccaagacc accagcggat ccgttctgct cgggggtaac tcgaacgtca
     4321 cccttaccgc ctcgaacccg aacggccagc cgaccggcta caacctcagc tttcgggacg
     4381 ttctcccgcc ggggatcagt tacgtccccg gttcctcacc gtccagcacc ggcgagccac
     4441 aggtgatcgc gaacgccccc gctgccgggc agaccacgct gatctggtca aacgtcgccg
     4501 atctcacccc caattcgacc ttcaccttca cttttcaggt caccaacagc ccggcggtct
     4561 tcgacatcgg cgacacctac cagaacacgg ccggcagcta tctgaactgt gacccgcgtt
     4621 acgtgccgaa gttcaacgcc cagggccagc cgacccagag cggcggaaac gccacctgtt
     4681 caggcctgcc tcaggagact tcgtacacgg gctcggcgac cgcttcggcc aacacgctga
     4741 tcaaggcgat cgagatcgac aagtccgagc cctccccgga ggggcagctg ctccggggtc
     4801 tgcacgacca ccagaccgtc tacacgctga ccgtcaccaa caacgaggtc gatgccgacg
     4861 acaacgtgaa ggtcgaggat tacctgccgg ccggcctcga gttcctcggt tgtggcaccc
     4921 aggacaacac gaccgacgcg ccgaccaacc cgggcagcgc ggaggagtac cccggctcgg
     4981 gcgcgatcaa cccgggcaac gctcctgccg cgccggattg cgtggtcccc gacaccgtcg
     5041 aaacggtgct caacccctca ggcttgccgg ccggggtcta cacgcatgtg gtctggaaca
     5101 acctcggcaa cctggggcct tccggccagc tccggatcca gtacgtggcg gcagtgccga
     5161 tccggcagaa caccatgacc tggaccaacg gtgttcctcc ggtaaccggg acgcagggtt
     5221 ccaacctcga caacaattcg ggtcccgaaa ccacccacct ccaggggctg accaaccacg
     5281 cgaaggtgac ctccgaccat cagggcccgg gcggcccgac cgaggtcact gactccgagg
     5341 accacaccgt tcaggcggtt gacatcagga tcgtcaagtc ggtcacgccg gccggtctgg
     5401 cggtcggcca gatcagcacc tggtcgctgg cgatcgcgac cagcgagtac cgctacttcg
     5461 aggatgtcga ggtcagcgac acgctcggcg acggctactg cccgctgggc ccggtcaact
     5521 acgagcacac cccgccgccg gcaaccgccg agtgcaaccc gaccggcaac aacccgtcgg
     5581 ttccctacac ctcggcggtc gagcagacca acggcacctg gcagatcacc tgggattcga
     5641 gcaccgctcc cgcactcggg acgatcaatc cgaacagcaa cctgacgatc agcttcccga
     5701 cgaagacccg cgagtacttc cagcagaact acgtgaattc aaccccggtc ctctccaacg
     5761 acaccggccg gaacgacgta gacatagccg gggccgcctg ggtcatctgt gcccccggtg
     5821 ccccctcgcc ctgcccgaac ctgagcccgg acaagatcga caccgacttc accgatggca
     5881 ccgttctcga tgacgattcg gacgcgaccc aggtcgggtc cgggcccagc atcaacaagc
     5941 aggtaagccc gaccgtcaac ggaaactgcg gcagcctgaa cgactacgcg agcggcatca
     6001 ccccggtcgc cagcccgggt gacaccatct gctggcgcct gaccatgtcg ttcccgaaca
     6061 acctgacgac cagcaacgtg ttcgtcggcg attacatccc gcccggcacg acctatgtct
     6121 tcaacagcac cgtggcgctg ccctccaaca cggtcggcat cttcaccggc tcgcccccgc
     6181 agcccgacct gtttggtacc tccggcctcc gctgggccct caacgacggc gggggtgaag
     6241 tccagcccgg caaggtcttc gacgtggtct tcgcaacgaa ggtccagcgc agcaccgatt
     6301 ccgtggacgg tgacatcgaa ggcaacctga tgaaggcggt ctacaccaac tccgacggaa
     6361 cctcgttccc gcttcgggac gaagaggatt tcgaaaggtc ccagcccgag atcagtctcc
     6421 tcaagggggt ctacaaggtc aacggacagc cgccggcggg caaccctcca aacgttgacg
     6481 gggccgtcgt cgacggaggc gatgacgtga cctatcgcgt cgacgtgacc aacgacggct
     6541 cggtgaacgc gatcaacacg caggtctggg acatcctgcc gccgcaggtc acctgtgccg
     6601 acgtcgacgc cggttcgatc tcgaacggcg gtacctgcaa cgcggggcag aaccggatcg
     6661 aatggtcggg gctgaccgtt cccgccaccg gcaacctgac cctgacttac accgttgaaa
     6721 tacctgatgg cttgagcggc ggggacgtgc tcaacaacac tgccggtgtt cgcgagttcg
     6781 cttcttccag tggttccggc ccctatgtcc aggtgccgga aaacaacatc gacccgaccc
     6841 tggatccgaa cgccaacgcg cccgcggcca aggatccctc aaacgtggtg atcgaatctt
     6901 caaccaccgt caagaccagg gtcaccgaca tcaaccagcc gggcaacagt ccgaccaccc
     6961 aggccacgat cggtgagcga atcaactnnn nnnnnnnggt gaccgccacg attccgcagg
     7021 gcacacgcct ctacgggccg accgccctgg ttgacaacgt gagctcccgg atgaccgtgg
     7081 ttccgctttc tgccagcgcc accctggaca acggcacggg cggcggacag gtgccactgc
     7141 cgacggccgg gctcactttg acccagaccg gcaacgtcgt aaaggtcgat ttccccaacc
     7201 cgtattcgaa cccggaacac agcggcgatg acgtgatcgt ccttactttc ggggcgatcg
     7261 tgcgggacgt ctatccgacc aactacgccc agggcaactc gacccagtac acgatgccga
     7321 ataccggaac cctgagctgg aaggacacca acggcaccaa ccgcagcgcc tccgggtcgg
     7381 tcaacaccac gatcgtcgag ccacggatct cgatcgacaa gacagtcgac gatgacggac
     7441 cggtgagtcc gaacgatctg ctcaactaca cgatccaggt ttcgaacggc tcggcggcta
     7501 gggtctcgac cgccaacaac acggtgctga ccgacaaggt gccggtcggt ctgacgccgg
     7561 ccaacggcgg agtgccggtc gccgatggtg gcaccgtgaa tccggatggc ggcatctgga
     7621 accttgcgac gcgcacgatc acctggccga cgatcccgac gatcaatccc ggtgcgatca
     7681 cgaacatcac ctaccaggcc agggtcgaca acccggccgt cggttccgac ctcctgatca
     7741 accacgccca ggcggcaacc acctcgatgc ccggcacccc gaccgtggag cgtgattctt
     7801 cgaccgcggc acccggctac ctggtttcgg acgaagtccc ggtcgaagtg agttccccgg
     7861 cgatcgtcaa gaccggcctt cccgcccagg cgacgatcgg ccaacacgtg accgtgaccg
     7921 tcttcgcttc gatcgccgcc aacctgacca actacgacgg aaccgtggag gatgaccttc
     7981 ccaacgggct gatcttcgac aattacgtca gcgaggaatg catcgaggga tgttccgggc
     8041 cggtcacgat ttccaccctg ggcgtggtgc cgaacgggac cggccagcgg atcggctggt
     8101 ggattggcga cgtgcccgcg gcgcccgaca gccgggtcgt ggccctcacc tacagggccc
     8161 atgtcgccga ccagtaccag gtgccgcccg gcggacctgt gctggacggc gatgccctgg
     8221 tcaacacggc gaccctgctc tacaacctga ccgacaaggt cacggtgccg ccgacctctc
     8281 cgccggatcc ctccaccttc gaccggagcc tcgatgacga tcacaccacg accgtggtcg
     8341 agcccgacct ggcactcgac aaatcggttt ccggtgacga tgaccaagac gattcccgga
     8401 ccacgcagcc cggtgacagc tacacgttct cgatcaaggt caccaacaac gggacttccc
     8461 cggcctatga cctgacggtc accgaccagc ctgacccggc gctgaccaac atccaggtca
     8521 accattcgcc ggtcttcgag gtgccggtcc ttcccgtcaa ttcgaatgat ccgatccagt
     8581 ggttcatacc cggcccgatc gatcccggcg agtcggtcac ccttacctac acggccgatc
     8641 tggtgccgtc cgcccagctg cacaacatgc agcaggtagt gaacaccgcc gagctgcctt
     8701 cctattgggg cgtttcggcc ggggaacgcg aggatccgga caacgccggc atcgactacc
     8761 gggagtacga gggcaacaac gacaccgtga ccatgacggt gctggtcccg accgttaccc
     8821 tcgacaagac gaccgggctg ccgcagttcc ccgacaccgg tgacgcccag accgagacgc
     8881 ccttcccgtg gcggatcctg atcaccaatc cgaacacggg cagcagcctg ctcggcgtcg
     8941 atctgaccga caccctgccg ccgaactgga cctatgtgcc gaactcggcc cagatcaccg
     9001 gcaccggcac cctcaccccg ggtggccagg tcaatccgac gatcacccag aacccgaccg
     9061 gtgacgtcct ggtctggtcg aacatcgcga atctggtcgg agtccagaac gccgttgtct
     9121 ccttcaacgc gatcccggag ccggaagcgg cgatcgatcc ggggctgacc aacccgcatg
     9181 tgaacgaggc cgaggcaatc ggggaagaca cttccggcgc taccgcctcc ggagacgggc
     9241 catacacgga cgacgatgac gccacggcga ccctcgaaac tccggtcacc gacatccaga
     9301 tcgtcaagac cgccgatgac ccggccccga cggcgggcac gaacacgacc tggaccctcg
     9361 tggtgaagaa caacgggccc aaggtctcgc ctgacgtcga ggtaagtgac gtcctgccgg
     9421 ccgggctcac ctacgtgagc gccacttcct cccaggggac ctgtgcggaa aacccggcgg
     9481 gaaccatgca ttgccagatc gggcagatga acgtcgatga cgaagtgacg atccacctga
     9541 ccacggcggt cggggccggc acggtcggcc agaccctgac caacccggcc gatgtcgacg
     9601 acccctacat cgacgattcg aacccggcca acaaccattc agaagacgac gtgatcccga
     9661 ccgagtcggc cgacgtcggg atcgggaagt cgatcgacga gccgctcttc gccggccaga
     9721 ccggcagcta caaactgacc gtcaccaacc acggccccag cgtcgcccgc ggagtgaccg
     9781 tcaccgacac gctgccggag aacctgacct atgccggcgc cacgaccccg gtcggcagct
     9841 gcagcggttc cggacaggac ttctcctgcg atctcggtga tctcgacccc ggccagaccg
     9901 tggtgatcga cgtgtcggtg aacgtgctcg aaggcgggga gtacacgaac tgcgccgacg
     9961 tggccagccc gaccccggac ccggacggcg acaacaacca ttcctgcacc accaacccgg
    10021 cggaaaacac cgacctggcg atcgagaaga ccggcccccc gtacttcccc gagggcaaga
    10081 accgtaacta caccctgctg gtgaccaacg tcggtgacca gccgaccggg ggcaccacca
    10141 ccgtgaccga ccgcattccc gatgtgctca ggccggaggc cgcctacggc aacggctgga
    10201 gctgcgcgat cagcggccag aaggtcacct gcacccgctc cgacgtgctc gagcccggtg
    10261 attcattccc cctgatcacg atcagggtgg ttgcccgcga aaacctgctc ttccggccga
    10321 tcggcaacac cggccacgtg cacctgccgg gagacccgaa cccggacaat gatcaggaca
    10381 ccgtccgcac gaagaagggc gcgatctgcg agggcgggtc tctggccctg aacccggcct
    10441 tcgtctgggt cggccagaag acgatcgtca aggcgaccct gcggacgaga gccggagctc
    10501 cggcgatcgg gctcccggtc cggatcaagg gcaacggcaa gggtggcgcc agcggcaagc
    10561 tgcggatcct gaccactaac ggcaagggca aggtccactt caccgcccgg gcgaccagta
    10621 cggaggcccg ctggacggtg agcgtgccgc aaagtcacca gaaggcggtg ctcgcaccga
    10681 agcgccagga aacctgccgg gcgatgaacg tgaccccgcg gacgatcgac atcaacaaga
    10741 ccacggtgct gaccgtccgt ctgcggactc ccgaaggccg tccggccatc ggcatcaccg
    10801 tggtcgccag gggtcagggc acgggtgact cggctcggac caacggcaag ggcatggccc
    10861 gcctcagggt ccgtcccaac ggtcagggtt acctcgtggt taccgcgccc aatgccacgc
    10921 gttgccggtt ccggatcggg gccagtgacc tggacgctgc cggcgccggt aaccagctga
    10981 ccggttagcc atggtccggc ggggtgtagt aaccggggtg ctggtttctc tttcgctgct
    11041 gctcgtctgg gatggctccg cagcggccag cgcagcgcgc cccgaagcct tcgcggagaa
    11101 ggtggcgcta ccggcggtcg gtgaggcgct agccccccgg attgcggtgc gggcgagacc
    11161 gatatcgaag gccccggtca gggcggtgat gaaggcgttt cgtttcgact accggcccac
    11221 cgtcttcttc gcgattgacc ggcgggaaac cggccagggc gagtggctca aggtcaggat
    11281 ccccggccgc ccgaacggtc gcaccggctg ggtcaggggt tcgcggattc gtctgctgcg
    11341 gagcgccggg ccattccgga tcgtgatcga ccgcagtcag cggcgcctgc ggctctaccg
    11401 ccacaaacga atggtcatga aaacgaaggt tgcggttggc acccccgacg caccgacccc
    11461 gctcggccac ttctacctga ccgcgggttt cgtccccggt gatgactttc tcggcccctg
    11521 ggccttcgag accagcgcct atgcggcaat taccgactgg ccgcgaggcg ggatcgtcgg
    11581 gctgcacggc accagtgaaa cctggtcggt cggccttcgc gccagccacg gctgcctgcg
    11641 ggtctacaac aaggtgatcc tcgccctgaa gaagcgggtc cggccgggaa ccccgctgcg
    11701 catccgcgca tgagctgccc cgtcgactgc ggatcctgcg actatccccg ctgatggcaa
    11761 tcagcgtcgg catcggatac gactcccacc gcttcgcgga tgaccgcagg ctgatcctcg
    11821 gcggggtgga ggttcctcac gagatgggcc tgagcggtca ctcggacgcc gacgttctga
    11881 cccacgcgat cattgacgct gtcctcggtg ccgccgggct cggtgacatc ggcgaggtct
    11941 tccctgatac cgatccggcc tgggaaggcg ccgactcgat cagcctgctc agcacggtaa
    12001 tcgggatgct gcgggggccg atcacaaacg tcgatgccac cttgatctgc gagcagccaa
    12061 agctgggccc ccaccgtccg gccatggtcg caaggttgtc tgaaataacg gggagccggg
    12121 taagcgtcaa ggcaaccacg aatgagcaga tggggtggat cggccggggc gaagggatcg
    12181 cctgcatggc tgttgccgcc gtcaatctcg actgatcctc aagtagagtc attcctcgat
    12241 gaacccggtc aagtcggccg cccaggccgt cgccaacaag gcatttgaaa tccgcaccct
    12301 gcacaaggcc ggggtggtcg gcgggctcgg cccggctgcg ctcccggttg ctgcctcggc
    12361 cctgatccgg ggcgggacga ctcccgcgac cgcgattcgc gtcgccgccg tcaagaacgg
    12421 ggaccgggtc tatctggagg atgaacgcgg ctccctgact ttcgccgaag ttcaccggcg
    12481 aagcaacgcc ctcgctcacg ccttcgccgg gctcggaatc ggccccggcg acggtctggc
    12541 cgtgatgtgc cgaaaccacc gcggcttcgt cgagaccaac ctggccggct ggaagctcgg
    12601 tgccaacgtg atcctgctca acaccatgtt ctcgggcccg caactttccg acgtgatcga
    12661 acgcgaaggc gccaaagccg tggtcctgga ccaggagttc gaggagctgc tctctggcgt
    12721 tccgggaggc atcggccggg tctgcgggtt cgaggatgaa ccgggcacct cgggcctgcc
    12781 cagcctcgag gacctgatcg ccgggaatga cgacggcgag ctgccctggc cgagcgaaaa
    12841 gggtcgcaac gtcatcctga cttccggcac cacgggcact cccaaaggcg cccagcggcc
    12901 cgccccgagc ggtctttcgg tgatggtcgg catgctcgac cggatcccgc acttctccgg
    12961 agagacgatc gtgatggcag cgcccctgtt tcattcctgg ggctatctca actcgatcat
    13021 cggcacttcg gtcggggccc ggctcgtcct ccagcggcgc ttcaacccgg cacggacgat
    13081 ggagctggtg gacgaatacg acgccgacgc gctggtcgcg gtgccggtca tgctccagcg
    13141 catcctcgaa ctggacgccg agaccagcaa gcacttcaag gcgagcaagc tggggatcgt
    13201 gacgctttcc gggtcggccc tgccgggtgg tctggcgacc gcctggatgg atcgctacgg
    13261 cgacaacatc tacaacttct acggttcgac cgaggtcgcg atgggttcga tcgccacccc
    13321 cgaggatctg cgccatgacc cggccacggc aggccggccg ccgtacggga ccagcgtccg
    13381 gctggtcgac gagttcggcg acgaggtccc tcacggggag accgggcgga tcttcatccg
    13441 caatgaaatg accttcgacg gctacaccgg agggggcaac aaggagaacc ttgacggcta
    13501 cctctcaagc ggcgacgtcg gccacttcga cgaagacggc cggctctaca tcgacggccg
    13561 cgacgacgag atgatcatct ccggcgggga gaacgtgtac cccgccgagg tcgaagacct
    13621 gatctccggt cacgaccagg tggtcgagtc ggccgtgatc ggagtcgatg acgagaagtt
    13681 cggccaggcg ctccgggcgt acgtggtgct gaaaacgccc gattcgctcg acgaagacgg
    13741 cgtccgcaag tacgtgaagg ccaacctcgc ctcctacaag gccccccgcg acgtgatctt
    13801 cctgccggaa cttcccagaa acgccacggg caagatcctg aaaaaggatc ttgcctgacc
    13861 gcggatcggg cgatccgtgg ggttgcgtag tagtttttag gcgatgagcg ataccacttc
    13921 agctccgtct gcggaagagg caggctccgg aaacgggctg ccccccgagc tgcgccaggt
    13981 cggcgaggcg tcggccaaac tcaactcgcg gattgaaacg ctggccaagg aactctccgc
    14041 cagcgagtca aaggcacgcg aggccgagat cgcttcccgt gaagccgaac tcaaggctct
    14101 cgccaccgcc cgcgactccc tgaccgaagc ccgcaaggcc aaggcggaag ccgaacggga
    14161 ccgggcggcc gccgcgaccg ccgaccaacg ggtcgaagcg gtcaaggccc aggccacccg
    14221 tgacgtcgcc gaggcccggg aagagatcaa gaccaaggcc cgcgaaaccc tgcgaaagct
    14281 gagcgccgag gcccgcgaga agatcgaagc ctcgcgcaag gcacggctcg aggcggagga
    14341 gaaggccaag gagcttgagc agaaggatgc cgaccagcgc gaagcccagg ccagggtcga
    14401 agccgacctg aagcgcgtcg aaggcacgct caagcaggag cgcgaaaccc gcgacgaggc
    14461 gatggcaaag gttgctgccg agggcgagga acgcctcgcg gcccgcaccc ggcaccttga
    14521 ggaggtggcc gagcggctgg ccaaggaagc ccgccaggcc gccgagcgcg aagccaagaa
    14581 ccggatcgag gccgccgacg agcggacccg caaggcccag gagagggccg cccaggccga
    14641 agcgcatgcg aatgaattcg agtcgctggc ccgccagcgg atcgccgaga tcgaggccga
    14701 cgttgaccgt cgcgtgatgg aaggcaccga gcaggttcgc cgcgaggcgg ccgagcgggt
    14761 cgaggagctg atcgagcgtt tcggccgcga agccgaggag aacgcacggc gacgcgccga
    14821 tgaacgcctg cgcgaagaag acgagcggat ccagcgcgag gcggatcgcc gggccgagct
    14881 ggcccgccag tcggccgagg atgacgtcca ggccgcagcc gaccgtgccc ggcaggaagc
    14941 tctccgcgcc gcctccagca ccgctccgga atgggagcgt ggcgattccg gcgaaggcga
    15001 cggcggaccg gcaccaaggc gccggcgcgg cgggcccggg gccggcggct accgcacctt
    15061 ctgagtagtc tccccgcggc aatgcgcgag gtcaggatca gggacacgat gagtggggaa
    15121 ctcctcactc tcggcaccga tgaagagatc ggcatctacg cctgcggccc gaccgtttac
    15181 agccggatcc acatcggcaa cgcccgtcct ttcgtgatct tctcgctgct cgcccgtttt
    15241 ctccggagcg agggctacgg ggcgaagctg gcgatcaacg tcaccgacgt aaacgacaag
    15301 atctacaccg ccgcggccgg gcggggggtc agctcgaccg aactggcggc cgagatgacc
    15361 gccgcctatt tcgaggacac cggaaggctc ggcatcggcc gccccgactc ggagcccctg
    15421 gcgaccgaga cgatcgggcc gatcatcgac ctgatcgcga ccctggtcga ggaaggccac
    15481 gcctacgagt ccggcggtga cgtctacttc gcggttcgga gcttcgatgg ctacggcaag
    15541 ctctcccacc gtccgaccga ggaaatggaa cagggagaag gcgatgacgc cggttcgctc
    15601 aagcgcgacc cgctcgattt cgccctctgg aaggccaaaa aagacggcga ggacctgagc
    15661 tgggaatcgc cttggggccc gggccggccg ggctggcaca tcgagtgctc ggcgatggcc
    15721 gaggccgaac tgggccagcc gttcacgatt cacggaggcg ggtccgacct ggtcttcccc
    15781 catcacgaaa acgagatcgc ccagtccgag gcggcccggg gcgagcagct ggcccggatc
    15841 tggatgcaca acgggatgat cgaaaccggc tcggaaaaga tgtcgaagtc ggtcgggaac
    15901 atcttccagc tttccgaggc gctcgacgcc tacggagccg aaaccgtggt cgcctacctg
    15961 atttccggtc actaccgcca gccactcgcc ttcggcgagg gcccgctcga acaggcggct
    16021 gccggcaacg aacgcctgcg caacttcctg cgcgaaaatc cgccggcagg ggcgggggag
    16081 ggcgtgcccg gcgacgaggc cgcggcggcc ggttcccggt tccgggacgc gctggccgat
    16141 gatttcaaca ctccccgggc gatgcgcgag gccttcgacc tggttcgcgc cgtcaatcag
    16201 cggcccgacg accccgccct ggccggtgcc gggaccgttc tggccgggat gctggagctg
    16261 gtcggcctgg aatccctcgc cacgggcggg gaggagggac ccggggacga ggcgctcgag
    16321 ctgctcgagg caagaaacgc ggccagggcc gacaaggact gggcccgcgc cgacgagatc
    16381 cgggacagtc tggccgaact cggctgggag gtccgtgacg gggccgaagg cgcccggctc
    16441 gtgccccgca gctgatgtcc gacccggaga tcatctatgg ccgcaatccc gtcgaggagg
    16501 ccaagcgggg ccggcgaaag gtgctccggg tctgggaagc ccccgaaacg ccggagacca
    16561 ggctggtcga gctttgcggt tcgaccgacc atcaggggat cgtggccgag gtcgagcctt
    16621 tcccctacct gagcgggtcc gacctgctgg ccggggagga cgcactggtc gtggtcctcg
    16681 accagatcca ggacccccgc aacctcggtg cgatctgccg ttcggccgaa gccgcggggg
    16741 cgaccggggt ggtgatcccc gatcgacggg ccgcttcggt caccgccgcg gcggccaagg
    16801 cttcggccgg cgcggtcgag catctgaaga tcgcccgggt ccgcaacatc agtgactgga
    16861 tcatcgaagc caaggatgcg ggcttctgga tctggggagc cgacggacgc gcagaccagg
    16921 ccccctggca ggtggacctg accggcaaga ccgccctcgt cctcggcacc gaggagaagg
    16981 gcctccggca acgggtcgcc gattcgtgcg acggcctgat cgcgatcccg caaagcggcc
    17041 aggtcgactc cctgaacgtc tcggtcgccg ctgccgtcct gatcttcgaa gcaatccgcc
    17101 agcgcagccg gacctgaacc tccggccccg cgccacccca tcaccattcc gccccttcgg
    17161 gcgggtcgga tttcccgcac aggcgacgga aatccgaccc acctcacggt tcctccctgg
    17221 acttcgcttc tgcaagccgg attgcagatt tggaggcagg tagttgacag aaccccgtgt
    17281 atatgtaaac taagcgacag ataaagggtg aacctcgact tgagtgtgag attcatctgt
    17341 cagcaagggc gtccagggga ggtaagtggc gccacaggcc ttcttctaaa ggagaatgtg
    17401 cggtggctac cccaacctca atttcccaca accatgcggt ttccgacgtt cctgctcgat
    17461 cccgcggttc agtatctaac cctcgacttg agcctgatga tgagaccctc gtcgccttcg
    17521 ccaagcgtgg caagaccgat gcggccgatc gtctgatcag gcggtaccac ggttttgtcc
    17581 ggctcaaggc ttcttcctac ttcctggtcg gcggcgattc cgacgatctg atccaggagg
    17641 gcctgctcgg cctgcacaag gcgatccggg attaccggac cgaccgcgag tcgagttttc
    17701 gcaacttcgc cgagctctgc atcacccggc aaatcatcac cgcggtgaag accgcgaccc
    17761 gcaacaagca cgtcccgctc aaccagtacg tgtccttcgg ccagtcgccg gcctcgaaca
    17821 ccgagtcgga gaccacgatg gaggagatcc tcccggggcc gaccgcgctc gacccggtca
    17881 accaggcgat ttcttccgaa gaactctcct ccctcgtttc ctgcctccag tcgaccctct
    17941 cggaaatgga aagcagcgtg ctcggctact acctcgagga ccgctcctac gaggacgtcg
    18001 ccgccctgct cggttgcgag accaagaccg tcgacaacgc cctccagcgg gtcaagcgca
    18061 aggtcagcaa gcacctcgaa tcacgcgaca tcgccgtctg aacaagcggc attcggcctg
    18121 acaggccatc gccccgcgcc ggaccgcacc cggccggggc caaagcctgc cggggctgtc
    18181 agaatcacgc ctcctgcccg gctagctcaa ttggctagag cacctgtctt gtaaacagga
    18241 ggtttccggt tcgagcccgg agccgggctc tcgctgtcag gagtcgttca ggttcgggag
    18301 tggattcttc cggcatgaac agccttgctc aaagactccc gacaaccggg gcggaccggt
    18361 ctctggcccg ccagatgctc aacaaggttc cggaggtaac cctctacttc tggatcatca
    18421 aggttctctg caccacggtc ggagaaaccg cggccgatta catgaacgtc gaccttggct
    18481 tcgggttgac caataccacc tatgcgatcg gcgccttgct cgtcgtagcc ctgatcttcc
    18541 agttcagatc cagacgctac gtgccgggga tctactggac tgccgtggtc ctgatcagcg
    18601 tggttggcac gttgatcacc gacaacctcg tggagaacat gaatgtcgcg ctccaaacca
    18661 cgacgatcgg gttctcggtg gcgttggcga tcacctttgt cgtgtggtac gcctttgagc
    18721 ggaccctctc gatccactcg attacgacca cccgcagaga ggccttctac tggctcgcaa
    18781 ttctgttcac cttcgcgctg ggaacctcgg ccggcgacca gatcgccgag cagtacgggc
    18841 tcggttattt cgtttccatc ctgatttttg ccgcggcgat cgggctcgtc gcgctggcgc
    18901 acttcaggtt caggctgggg gccgttacag ccttctgggt cgcctacatc ctgaccagac
    18961 cgctcggagc ttcaatcggt gactggatgt cccaaccgac cgcgaacggg ggtctgggcc
    19021 ttggaaccac cgtgaccagc ttcatcttcc tggccgcgat cctcgggctc gtgatctacc
    19081 tctcggtcac aaaggttgac cagactccac cggaggtcgt cgaggccgaa atggaggaga
    19141 cggaggaggc ggaggcggtc agcgtttagt caggcgccaa ctcaggacgc gatcggaacg
    19201 gtcaggatga ccgttcctcc gtcgcccgac acggggatcg agacgtctcc accgttggcc
    19261 cgggccaggc gacgggcaag ggcgaggccc agaccggtac cgggcggcgc cgcttcgttt
    19321 cgtgccgcaa ttccccgcat cccgggctcg aacgccgcct ccagatcccg gggcgaaaac
    19381 ccgggtccgt catcccggat cacgacttcg gccgttcttc ccctcgaccg gattgcgatc
    19441 gacacggtgg ccctggcgta ggtgatcgcg ttctcgatga tcggcgagat catccgctgg
    19501 aacgtgtcgc cggccacctg agcccggatc cggccttcgg gccggtccag ctcgaccacg
    19561 acgccgtatc gatcggtcag cgaacccgcc gcggccactg cggaatcgac cgagtcaagg
    19621 agatcggccg cttcatcgga tccgccgctg ccttccgatc tcgcgacgtc gagcaaggtc
    19681 tcgagaatct tcgtgagctc ggtcgatcgc tcggcgatcc gttccagtga ttcccggtat
    19741 tcagccgcgt cccgatcctt tgtgagcgcg atctccgcct cggccgatat cgcggaaaga
    19801 ggagtcctga gttcatgcga gagttcggcc gaaaagttcc tctcgtgccg gaccatgtag
    19861 gccatccggt ccagcatcgt gtcgaaagtg gccgccagcc tggtcagctc gtcggtgggc
    19921 tcgccctcgt tgaaccttcg gtccagatcg tgctcgctcc atttcgcggc ttcctcggtc
    19981 atctccgaga cgggcttgag tgccctgccg accacgagcc tggtcgtgat cgtgatcagc
    20041 acgagcatga tcaaggcgag caccaccgaa gcgaaggcgg cccgatccgc ggtccgctcg
    20101 taaggctcga gcgagacccc ggtaaccacc gtgcccacta cccggccgtt ctgcaccacc
    20161 ggccgcgcga agagccttgt gtcgctgtcc gagtcatccc gctccccgct tccctccgcg
    20221 gcgagtgagg ccgccacccg atccagctgg ctggcaacct gcggagactc aagcggcttt
    20281 cggtcgtcgt agatccagac cagcgcgtct tcggcgccgt cgtccgatcc ctcgctgact
    20341 tcgagtttcc cgttctccag atcgatgttg tcgagcgtcg cctgggccct ggcgtgaagg
    20401 agccggtcca cgtcgccatc gagactggca cgcagcgaga ggttgaagcc caccacgagc
    20461 agggccagca tcaccgcggt cacgcagacc accgagaaag tcagtcgctg acggatcgtc
    20521 cctttcattc gagccggtat ccaactccgt gaacggtgtg gattctcgac tcgctctcga
    20581 gttcgtcgag tttcctgcga agcctccgca cgtagacatc gaccgtgttg tcgtgcacga
    20641 tcgcccccgg tggccaggcg gccgcgatca tctcgcggcg tcggatcact tctcccgatc
    20701 gggcgatcat ggccccggtc aggcgaaact cggtcggagt cagttcggtc tcacggtccc
    20761 cgcaggtcag tacgtgccgg accggatcca gcctgagatc cccggaagtg gaccgatcgc
    20821 cccgagtcaa acgcctgccg agcacccgca gccgggccac gagttcggcg aacacgaacg
    20881 gcttggtcag gtagtcgtct ccaccggcgc tgaagcccga gacccggtcc ggtccggaat
    20941 cctttgcggt caggaacagc acgggtgcct cgatcccctt cgacttgagc gcctggcaga
    21001 catcgcggcc atcggaatcc ggcaacccga tgtcaatcac cagcaggtcg tgcttttgcg
    21061 gatcggcaag catcagggct tcgcgaccgt tgcgcgccag atcgacctcg aatccctcct
    21121 cgtcgagccc ctggttgagc agccgccgga gctcggggtt gtcctcgaca accaggacgt
    21181 tcaggttcat gtgatcgccg cttcggtcat acggtccaat gatcggtggc aaacctgaac
    21241 gactcctgaa aatccgctcc cggtcaggag aaattcaggg ttccgcgcac atactcgcct
    21301 cattggaaaa gcgccgatgc ccctcggccc cgatgagaag gaagaacaca tgacatccaa
    21361 gatcaagaaa atactcgcgt cactcctggc gcttgcggct ctggccctcg gcggtgccgc
    21421 gatcgcttct gcaaccaacg gcggctccga cgacgatcac ggcgcgcagg agaaggctga
    21481 agtcaacgat cacgactctg acgccaacga gaacgagtcc acctctgaag gcgctgacga
    21541 caacgaagcc gaagacggtc aggaagcagc cgaccccggt gaagccgttt caagcacggt
    21601 cgccgcgaag gctgaagccg ccgcggttgc caggaccggc ggaacggtcc aggaagtgac
    21661 cgccgagacc aatgacgcgc ccgagaaggg cgacgatcag gacccgggcg agaaagccgc
    21721 tccggccggt accgcctatg aagtagacct gaccaagggc tctcaggagt tgaaggtctc
    21781 gctcgacagg cagttcaacg tgctccaggt tcagaccggg caggatgact gaacgtgtca
    21841 agggcggagg aggaacccgg gtcgactccg gcccgggctt ctcttgccgc gtttacccgt
    21901 gccggtcgac gttgctgaag atcccgacgc agccgctcag tcgaccagca ggcagacgat
    21961 gctgactgcc ttctcgacgt cggggctgtc ctcggtcagg gcgtcgttgt agatgaagcc
    22021 ctcgattatc cggatcgccg cgtaggcgag gtcaccggcc ggggcgcgca acccgaggtt
    22081 gccgctcgcc tcctcttccc tgagcacttc tgcgaagcgc tcgaccacgg tcgactgaac
    22141 ctggctgccg cgggtcagga cgaggcggaa ggcgagttcg gtttcgctgc ggacgaacct
    22201 gcggtgcggc tcgaagttga cgatcgattc gacgaacccg cgggtgaaga gctcgatccg
    22261 cctgaccccg gtcgccccct ctgacgcttc gcggccgatc cgggccgcct cctcgccgaa
    22321 gaaacagagg atctccgaga gcagcttgtc ccgggggccg gtccagcggt agagggtggt
    22381 ctttgaaatg ccgagatcct gagccagctt ttccatgtcc agccgggggc cgcggacgaa
    22441 tgcccgcagg gcggcgcgga aggcgtcggc cggggtcgcg cggggtgcgc cgaggccggc
    22501 agcttcgtcc tggtgggcgg cgaatgcctt cgaggttgac gtggtcgctg cttccatcga
    22561 atcgaactct acagatcggt gttacggaaa ggtggagttg cacgaaagtg tacgaatatg
    22621 atatttgtac acaatcgaat cggaaacacg acgcgtctac ttgcgccgga aatggagaga
    22681 gttgggatga aaagcaagct gatcgcggca ggggccgtgc tcgtcgcagt acttctgacc
    22741 ggggttctgg gggcgggagc ggcgtcggcc agctccgcca agccgccctt ctacccgatc
    22801 gacaagaaga cgatcaaggt gccgaacgac gtccagccct ggttgccgac ctacagcccg
    22861 gacggcaagc acatcgtctt ccagaatcag cttgacggca gtacctgggc cacctcgaag
    22921 gacggaaagg gcaccagctg catcacctgc gagttctcgg accggcccga catcagcggc
    22981 ggcttcactt atgtcttccc ggacaacaag cgtctcttcg tgagccatga actcggcggg
    23041 ctcaccgggg ttgacagcgg ccccaatacc gacgcctgga ttctcgaatg ctcgccgagc
    23101 atctacgact gccagagcca ccagtacctg cccgtcgaca tgtcggacga caaggttggc
    23161 gtgccgctga tcgttcagcg acggacctgg cacctcgcgc cggacggcgt ccacctcggc
    23221 tggatggaca tccggctcga cggcaccgtg atgatcgtcg gtcgtctgac ccggctggac
    23281 gatcgctaca aggtggttga ccagaaggcg gtcaatccgt accggccgac ctcgctgacc
    23341 gataccgacg ccgccaactg ggccggtgac aaccagctct gggagctcaa gagcttcgcc
    23401 gacggcggcg cctcgatcct ggccctgggc ggtgccggct acaacatcga cccggtcaag
    23461 atcaacctga agaccggcaa ggtcacccgc ctgaccgcca accccgactg ggacgaggac
    23521 ggcgccatct cgccggacgg caagatgctg gctctctact catggcgcac ccgccatcgc
    23581 ctggacgcct tcggctggat cccgcagatc gattcgtttg tcgagatgcc ggccggggcc
    23641 gcactcgctc ccttctacgt ctcgacctgg cccggcttcc agtgtgacct ctcgccctgg
    23701 ttgctctcct cgtccggcga catgggcggc aagctgatcg gccagccgct gaacgtctac
    23761 ccggaaaaca acctgacccc gggcaacaac ctttcgggca accagttctg gagcccgaac
    23821 agccaggacg tcctgctgca ggagcgcacc cggacaatcc cggcgaccac gctctcggag
    23881 catgttcagc agaagggcct gactccgaac cggatcgcga tcgcccacat cgagcgtccg
    23941 gccggcaaga agctgaaggt cgtttcctcg gaggtcggca actgggcggt cgctcccacc
    24001 cagtacttcc cctcggtcgg gtcgaacacc tcggtcaccg tgaacggcaa ggccggcggc
    24061 accgccgacc tggtctacac cgggaatctc cagaccggaa gctggtcctc cgagtaccac
    24121 gactacacca aggacggcaa gaccttcgtc aacgggacct acttcgtcag ccgcaccatc
    24181 gaaggggtct ggaccctcaa ggccgatgtc acggtgaccg gccagcacac gggccggctc
    24241 aacatggaca tgacgatcca gggcggcgac ccgctgccga tcatgaccgg cggcatcact
    24301 gctgtctatg acggcaagaa ggctccggcc ctgcccaagc tgggcgcctg ctacaagaaa
    24361 ctcccgcaga agagcaggct cgccgcccaa gtcaagtcga agcgcgccgg caaggcgacc
    24421 aaggtgaccg taaccgtcac cgccaacgtc tacggcgaca ggcgaccggt ccagaacgcg
    24481 aaggtgaagc tcgccggccg gatcgtccgc accgaccgct cgggcaaggc gaagctcaag
    24541 gtccgcggcc gcggcacgcg gaaagtgttg gtcaccgccg gggacacctt catcaagacc
    24601 tcgaaggtcg cgcacctgaa gcagcgctga ccaggcaatc ctcgtccctt ccgggtcctg
    24661 tcgggcaacg ggccggccga ttatggccgg cccgtttttc gtgggaggaa gccggcaact
    24721 agcttgtggg aatgcccgtc ggctacctga tcacgctttt tctgctcgga acctgcgtgc
    24781 tccttctgct ggctccggtg cggaagcgac cactcggcct ggtctccttc tggggcgggc
    24841 tcctggtcaa cgagcaggcg cactggttcg ccgtgctgct tgccggttcg accgtgctgg
    24901 ccttcgtcga gggcgacatt tccggtgtcg cccgggcgat cgcggggctt gccgtactgg
    24961 ccgaggccgg actgatcgtc ctctttctca ggtcgtttcg gtcccgcggg accttgcggg
    25021 aagcgctggc cgctttcgat tcagattcac ccgccgcact tgcaggccgg cgttcatacc
    25081 tggcggcgct tgccgctccg ccggcgatgg gccgaagtcg ggttcggctg aaatccgaca
    25141 ttccctacgg accggtcccg ggccgggcca atcggctcga tctgtacctg ccccggcacg
    25201 gcaaggtgac cggcccgacc atgatctata tgcacggggg caggttccgg tcgggtaaca
    25261 agcgaaggga ggcccaggcg atcaagtgcc agctggcccg ccgcggctgg tcggtgatca
    25321 gcgccaacta ccggttgaat cccgaaggat cgtttcccga ctacctgatc gatctcaaac
    25381 gcgtgatcgc ctgggcccgc ggggaggggc aggcattcgg catagatccg gagcggatct
    25441 ttctggctgg cggctcggcc ggggctcaca tcgcggcgat ggccgggctg accgcgggca
    25501 agccggagct gcaaccggga ttcgagcagg tgaacacggc cgtctccggg gtgatcggga
    25561 tctacggcta ctacggcggc cttgaatcac ccggcggccg ccccccggct gtcccctcca
    25621 gcccccggcg ccacgtgcag ccgggcgcac ccccgtttct gctcatccac ggagaccacg
    25681 acagcgtggt cgggcggggc ggagccgcgg ccctcgtcaa ggaactccag ggcgccggca
    25741 accaggcgtc actggccgtc ctgcccgggg ccgaacacac cttcgacctg ctgcgctcgg
    25801 ttcgcaccga aaacacgatt gactcgatcg tcgattttgc cgcctgggca gaacgttcgg
    25861 aaaatcgtga tctttcacaa tcccggcgct aagatgggct gttgtggggg cgaagggagc
    25921 aaaggggaat cttcccggcg ggctgcggac cggcctggtc ggtctggggc tgcttgcgct
    25981 cgcgattacc tgtctcctga cttcgcctgc gcccgataga tcgaacgccg agactcgggc
    26041 caacgctgag ccgcaagggg gcatccttcc ctgcggcagc cacgtggtcc ttaccgcggt
    26101 ctatcgcaac agggacgggc ggatccattt cgaaggggtc gccgatccga aattcgccgg
    26161 caggcaggcc cggatctatg accgggacaa ccatctggtc gcagcgacga ccgtgggcga
    26221 ggacggaatc ttctgggcga acgccaattc aaaggacgag ggttacacct ggctcacccg
    26281 gttcatcgcc agggtgggtg gtagcgagtc ccgctggaaa cggatcggcc aggcggtcgg
    26341 cctcagggac cggcagccct ttgaactgga gccgacggca cgcaaccacg gcaagaccct
    26401 ggagcgcacc agggtcaggg tcaaggtggc cggcggccac agcaatctcc tggtggtcgg
    26461 tctgcaggtc ggctgttccc gattcgacgt gatcgaactc gcttcgacct acacggactc
    26521 gaacggggtg tccgacctga acctgccccg tcccccggcg gatcgtccct actggatctt
    26581 ccgagtcagc accgaagacg actggaagat ctcgcccccg atcgtcgtca aatcggtgcc
    26641 ggaatccggc ccgaccggct gattcgcccg ggcccgcccg aaaacggcga ggacccgttg
    26701 taaccgggtc cgggggtttg tgtaggactc ttttgtgact caggagcaac tcgaggccta
    26761 ccacgagtac acgcgcaagc acggggtgaa ccgcggtctt tacctcttcg cgcgcatcgt
    26821 tctgcagccg tttttcctaa tctacctgcg gctttcgcgt cagggcaggg gtaacgcgca
    26881 gatcaagggc gcgaccatcg tcgccgccaa tcaccgcagc ttcctcgacc cgttcgtgat
    26941 cggcacctgc ctgccctgga acaagccgat gaattacgtg gcgaaggtgg aactgttcga
    27001 gaagcgctgg caggggttcc tcctctgccg ggtcggggcc ttcccgattc gccggggaga
    27061 ggctgacgag ctggcgatcg aaaccgccga gctggttctg gcccggggcg ggacgatctg
    27121 catctttccc gaaggcaccc ggatcaggaa gggttcgctc ggcaagccga agcggggcgt
    27181 cgggcgtctc gctctccgga gcggggcacc ggttctgccg gtcgccgtca ccggttccga
    27241 gaacgtgcgg cgcggctggc agatccggcc gcgcaaggtc gacatccggg tcggccggcc
    27301 gatgtccttt ccccagtcgg aatcgccttc tccggccctc gccgcatcgg tcaccgatcg
    27361 catctggccg aacgtcgaac ttcaatggga agacatcggc ggtctgccgc cgatgcgccg
    27421 ggccgcggtc atcggagccg gcagctgggg caccgcggtc gcggtgctgc tggcccgcgg
    27481 cgggctcgaa gtccagctcg gcacccgcac cgccgaccag gcggaggaga tggccctcgc
    27541 gcgcgagaac accaggtatc tgcccggcgt tcgcctgccc gacggcatcg aagtccgggc
    27601 ctccagccag atcgaactcg gtggagtcga cctgctctgc atcgcggtcc cgtcagcttc
    27661 ctacccgcag gcggtcggag cggtcgccga ccgggtcggt cgccgggccg ggggactgct
    27721 tctcgccaag gggttgatag cccccaaggg tcaactgcca tccgaatacg tgggtgagcg
    27781 gatccgctca cgcggcgtct cctgccttgg tggtcccgca cacgcaaagg aagcggtgtc
    27841 cggttcggcc gctctggttc ttgg
//
LOCUS       DAOQKN010000008        27645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_697_length_27645_cov_6.151142, whole genome shotgun sequence.
ACCESSION   DAOQKN010000008 DAOQKN010000000
VERSION     DAOQKN010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 27645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27645
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_697_length_27645_cov_6.151142"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="PLJ64_01160"
     CDS             complement(<1..271)
                     /locus_tag="PLJ64_01160"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC92131.1"
                     /translation="MSAQDKPPFPPLDLADRVSSLPETDNALDHFEWLGLMTRQSIDA
                     ALPEDWNYEGKRLLDFGCGSGRTLRHFLDVAEVAEAWGCDIDRPSI"
     gene            complement(294..1253)
                     /locus_tag="PLJ64_01165"
     CDS             complement(294..1253)
                     /locus_tag="PLJ64_01165"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC92132.1"
                     /translation="MAPGKRVTDPLQWGYSLSNVFELLSGCLEAIETKTVLEIGSYKG
                     ELTVELLEWAQTRGVKVAGVDPLPPDELEALAKAHPELELLRQTGQEVLREREFDDAI
                     IIDGDHNYYTLSEELKIIGERAPGAGMPLLMFHDVCWPHARRDTYYEPDRIPEDQRQP
                     LVHNEGIAPGIKGTAEWGLPFVWAAAEEGGEKNGTMTAIEDFLAERPGIQLAVVPAFF
                     GFGVMWHRDAPWASAVERVIAPYDRHPVLERMEGNRVEHLVAGQARARQLVKLEEKVA
                     KQEDLLRRLLGSSAFTAAEKLSGLKQRGKPLFTREEVQSLLDE"
     gene            1284..1883
                     /locus_tag="PLJ64_01170"
     CDS             1284..1883
                     /locus_tag="PLJ64_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92133.1"
                     /translation="MDDLGQHNDAASLMADLRSRHPRFTEAVLADTVVTAQHRGERYE
                     FRGKGDALVAALRLAWVSDAFLAQVLYRAKAACQRREVRVLPRLFHRLSMMLAQVSIG
                     DPVVMHPGVYIVHGQVVLDGIVEIHPGVVISPWVTIGLRAGNVVGPTVREQVNIGTGA
                     KLIGPIDIGAASSIGANAVVVDDVPAGTTAVGVPASQPG"
     gene            1996..3066
                     /locus_tag="PLJ64_01175"
     CDS             1996..3066
                     /locus_tag="PLJ64_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92134.1"
                     /translation="MGIFRRKDRGAAAVAEAEPELNPVVEEARALEADGRIFDGIDLL
                     TRTNRGSGNVEIEREIIAMRHRAGAEMVEAGDQHPVHPEPADYDFRLEDKVPTVTPAE
                     LTPEILRAAILKYGCLKVTGVMDEATAKSLADDIDHAFEIRQRLSDAKEVDPTRIYEE
                     FAPNEPYQVQYVERHWVALAGGVLAADSPKMFFEMVDSLEQAGVRDVVEGYLGERAAI
                     SAQKCTLRKADPSVKGGWHQDGKFLGDVRALNVWLSLSRCGDVAPSMDIVPVRIDEFV
                     AAGVNEGDLHYQVTDETAAEAAGETGIVRPIFNPGDVLLFDEMFLHQTGSDPSMPNPR
                     YAIESWFFGPSAFPEEYAPIAF"
     gene            3126..5897
                     /locus_tag="PLJ64_01180"
     CDS             3126..5897
                     /locus_tag="PLJ64_01180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC92135.1"
                     /translation="MVEPEFDSEIELGLEVKIPGQLPAGRADALFLYGYAFHRRQDVE
                     GLEILVDGQPNRAVGRMPRLDLFKSLHPLIPEGEVSETHDAGSLEDPELKSFRSGFWS
                     TVEIPATGPGKVRIEVALKLADGSISRAPLAEIEMVDQEQRFSTDIEEDRGSRPPEAR
                     IAICMATHNPDIGLLRIQLDSLREQTVTDWVCLISDDCSLPETFREIEAEIEGDDRFL
                     ISRSEARLGFYRNFERALGMVPETAELVALCDQDDRWYPEKLAELEQALGGKGAGKSG
                     RRGPQLAYSDQRLVTEDGQVIAETYWSGRRNNHTNFASMLIANTVTGAASLFTREMLE
                     YALPFPEAPGEQFHDHWLALVAMSTGGIAYVDKPLYDYVQHGSAVLGHDAANAGIKAG
                     RPKRPKHLSIGAVRQFFSGWRSAYFNAYLRLSVLARVILARCDGKLSGRQRRTLKRYV
                     KAEKSPAGFLWLLFRPVRELFGRNETLGVERLLIQGILWRYIIFWRNRGLEKPVGSLY
                     DASLPPAGRAAKAPAHNHPQTAHIQRMVRPLDLAVSEEAPERVNLLIPTFDLKHLFGG
                     YIAKFNLARKLAEAGFRTRIVTVDPTPPLPRSWREQVESYQGLEGAFENIEIFFARDS
                     GEALEINPRDRFVATTWWTAHLANDALSRTEHDHFLYLIQEYEPLTFVTGSWYAMADA
                     TYDFPHRALFSTELLREFFRDRGYGVYREGAATGDELSVSFQNAITPVSAPPAAELSA
                     RGTRRLLFYARPEAHAARNMFEIGLMSLQQAVEDGVFGPEWSFHGIGTVEGQSRLRLT
                     DEVALEILPRTSQADYGELLAGHDLGLSLMLTPHPSLPPLEMASAGMLTVTNTCENKT
                     ADKLTAISPNLIAAPPTVPGVVAALAEAAAAAGDGERRAAGSSVDWSSDWETSLGPDV
                     MDRVIGFLR"
     gene            5894..7204
                     /locus_tag="PLJ64_01185"
     CDS             5894..7204
                     /locus_tag="PLJ64_01185"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC92136.1"
                     /translation="MTPDSMTTDPLAETKTRDITENPVVISVEGLKKSFKIPLHRVES
                     FKERAVHPLRKTEYRELKALDGIDFEVRQGEFFGIVGRNGSGKSTLLKILASIYRADA
                     GKVRMAGRLAPFIELGVGFNHDLTAHDNVIMNGVMMGLSRQEAASRLDRVVEFAELED
                     FKELKLKNYSSGMLVRLAFSLMIQADADIMLIDEVLAVGDASFQQKCADVFNQMRDAG
                     KTIVLVTHDMAAVESFCDRAMLIEGGHVIEAGEPRDVARAYLKLNFDAETPRQETLGF
                     LEDGEVEDDFIPGVDIQAKLIDAWLDDGAGERVTNVEIGDPLRLNLEIEALRDLERPL
                     FTFQINDAKHTQVTGFNQTLEVEGPGDVIHEGQKVTISGEIVNRLTPGRYLLTCWVTR
                     NRETSERVVQGIKLFEFVIFGMKRTPGLVEAEASLNAKVAGSNG"
     gene            7197..8090
                     /locus_tag="PLJ64_01190"
     CDS             7197..8090
                     /locus_tag="PLJ64_01190"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC92137.1"
                     /translation="MAEQTATAAASPEEAMVSSPDSPELREVTGPTALGGGWRRSLDL
                     LYLISINDFKKTYFDTVLGYVWSLFRPLILFAVLLFVFTQIFRIGDDVPHYPVMLLFN
                     IVVFTFFQEATIAAVTSVVAQEGVVRKTQFPRVVIPLAVVLTALFNFGVNLVVVLVFL
                     LASGVPVEWTWLFFPLLVLVLTVITTAVCLLVSSLFVRFRDVGIIWGVLATALFYATP
                     VLYPIEVVPERFHDIVLINPLTPIFIELRHLVIDNSAPTAVSAAGGWLGLLPAVAVTI
                     ALCLLAVKVFSREAPRIAEEL"
     gene            complement(8111..9193)
                     /locus_tag="PLJ64_01195"
     CDS             complement(8111..9193)
                     /locus_tag="PLJ64_01195"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC92138.1"
                     /translation="MAEQGKSTDEKIDAVREGYESTLSWRVTRPLRSISDAARRLRGR
                     ETEIPWAEVMVEEKVEPDWTSWLDTFFGEELERIDAACAETPPEEAWQLFRGLGDDLW
                     AVLLTREYDLYPNIKALLPGAPEPGLQWMWNGATGLELLGQSKNFYARVRELNERHGR
                     APLDGARILDFGCGWGRLTRFFARDAMPDRLFGCDPTPEILEVCAETGVPGELRQTGF
                     LPERLPFENLDLAFSFSVFTHISEAAHEACLKAIHAALAPGGLAVITVRPPAYLDHDP
                     LMEAARAELSDDFASREMKEPRFIFVPHPVEDGHPQYDEGEMTYGDTVISLPYIEENW
                     TGLFEIVETGFLTGDMYQVPIALKKR"
     gene            complement(9218..10663)
                     /locus_tag="PLJ64_01200"
     CDS             complement(9218..10663)
                     /locus_tag="PLJ64_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92139.1"
                     /translation="MTHPRELIRRTFGNPDEGTAGRKARLAGLIGVPVAGAFLIGVIL
                     GVIGQSLSAGATFILLAIPVAIGGGILTGRAVLGGAMQLRGNQKAGWITEKLEPLNVA
                     ADPGQPLRVEIIHPAVDLKHFFGGFIAVFNLARRLGEGGHRVRIVTLEPLDLPDDWRR
                     QVAAYQGIGDSVGDLEVIDGSDRAVPVAMNPDDRLIATHWTAAHIASAAAAELDQESF
                     TYLIQEYEPLIFPMGSAAALADQSYELDHNAIFSTGFLRDYFRDQGIGVYRPEGPGEA
                     GAAVFDNAITPAATVTTGELQGKDRRRLLFYARPEAHAERNLFELGIMALDRAILAGH
                     FRGWDLAGIGNVDGTGELTLPQSKARLRMLPRVAQREYADTLRQFDVGLALMYTPHPS
                     LVPLEMAAAGLVTVTNTYANKDRASLEAISGNLIAAEPRIDQIEEALASAEVMSRDFE
                     ARVEGSKVGWPDNWDQALDDATMERIGRLLD"
     gene            10696..11685
                     /locus_tag="PLJ64_01205"
     CDS             10696..11685
                     /locus_tag="PLJ64_01205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNC92140.1"
                     /translation="MDLSLIIPAYNESEALPGLIAEIDSACEGLGLQWETIVIDDGST
                     DGTFGVISEIAKSNPRVGVVRLRRNFGKSVALAVGFDVTRGEKVITIDGDGQDDPADI
                     PRLLEGLENGSDLVSGWKSDRQDPVTRRLASRIFNWFTARVTGVDLHDMNCGFKAYRG
                     ECARSIEVYGEMHRFLPAFAAQQGWRVTELPVNHRPRLHGSSRFGLERYRRGALDLLT
                     VAFLGRYQNRPLHLFGLLGLVLGSLGFLICLYLSIIWIGGEAIGERPLLFLGVLLIVV
                     GVQLFSLGLLGQMMVVGRRAGVSEEKVLIEKVLSPESDPELGLSRLEAVGSGR"
     gene            complement(11651..12268)
                     /locus_tag="PLJ64_01210"
     CDS             complement(11651..12268)
                     /locus_tag="PLJ64_01210"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC92141.1"
                     /translation="MAANSTETVDTTNYEKFQTGNPVVKRLIGGFYDTVEEVVKPLKP
                     ATVLDAGCGEGETLDRLSEVLPPAPTGIDLNPESVEFAARRLPGATIRQADLLNLPFE
                     DGSFDLVFCLEVLEHLPDPAAGLAELARVSGRDIVVSVPHEPWFRLGSFARGKYLKTW
                     GNHPEHVNHWNPKSLKAFLSTRTEVVEVQTSMPWIIARCRPLPAG"
     gene            complement(12280..13143)
                     /locus_tag="PLJ64_01215"
     CDS             complement(12280..13143)
                     /locus_tag="PLJ64_01215"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC92142.1"
                     /translation="MLGASGFFGSRIAAELAAAGIEVLGPERSESELLDPAGLSELVA
                     ATAPAAVVNAAGMTSPASARANPSACFAVNAGGALNLLEALRQRAPGAQLVSLSSAAV
                     YAGSAPFDESSATAATTPYAASKLAMETICGQYRRGYGIPVTVLRCFNLIGPGEPSTQ
                     ASSEFVRAALAAGTGGRAEVEVGEPATARDFTDVRDAARAVRRVLEEEATGTLNLCSG
                     KATSLAELAALTGELTGVDLTLRGSGTGRPASGLLSIRGDFSKLRELTGWRPGITLER
                     SLTDLIESLRP"
     gene            complement(13179..14126)
                     /locus_tag="PLJ64_01220"
     CDS             complement(13179..14126)
                     /locus_tag="PLJ64_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNC92143.1"
                     /translation="MTVSVVTGGAGFVGSHLCDALLEKGNKVICIDNLDTGSLQNIEH
                     ITNPDFTFLPQDITEEVHLDGEVDFVYHLASPASPIDYARLPLHTLKVGAQGTWHMLG
                     VAKFKRARFLLASTSEVYGDPQVHPQEESYWGHVNPIGPRGVYDEAKRYAEALTMAYH
                     RQQGVDTSIVRIFNTYGSRMRAHDGRAIPTFMRQALQNQPLTVFGDGSQTRSFCFVDD
                     LVRGLIALAESGEHLPVNIGNPDEYTLLQLANAVIEASGAESTITFEPLPTDDPQVRQ
                     PNIDRAKELLGWEPTVPLSEGLKKTIEQSGRDRLIGKTG"
     gene            14213..15220
                     /locus_tag="PLJ64_01225"
     CDS             14213..15220
                     /locus_tag="PLJ64_01225"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNC92144.1"
                     /translation="MSAVRRKAIGVLLLAGLVLFCLPAASGAVPAWQSAPVIPSLSNG
                     DQARLKDRVAAGERTGLRSGVFAKVGDSNTEFSPDLYGLACRKPTGLSAALRGTLATY
                     SRVRLPNARALPGCRPWTSFSRRSSAAQAGVHSTWPLTPVKDLPDESYWAKPPGCPLK
                     GTPLSCEIDATMPRYAMVMLGTNDIGMDIAFGIQPGSRIVSRLGGVVRALLARKVVPV
                     LSTIPPIIRPDGTRQGEVTAGVARSNAGIFELARKWHLPMINLWRALKEPSMIDLGLA
                     GDGLHLSVAGAGGEMVGVQPGASTFADSVDFTAAGLRYGANRRNLIWLKTLARLDRVT
                     G"
     gene            15222..16262
                     /locus_tag="PLJ64_01230"
     CDS             15222..16262
                     /locus_tag="PLJ64_01230"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNC92145.1"
                     /translation="MGRRFTAVLLAGLILLAGCGSSGEDSGQAPDPSRAPARAGESAR
                     TPQPVADLSSGSVLPDLSGAAGDRLRREVARADERKLNPDVFAKVGDSNTEWPQNLYG
                     LGCREVGYGSNGQLEAVVKKYSKVRFPGLVSLDGCSPVNSLTRRSAAAVSGVWTEWLM
                     TPTEDLYATEVGPPTDECRPEQTPLDCEIELIRPRWALIMSGTNDALLGKPLGDTYRG
                     LLTKMVSRVRELGSVPVLSTLPPMAVLTENGQPGEERIAEANRIIREVASTEQVPLID
                     LHSALVAPGMVNQGLGPDGLHLGVYGGEAQPDVMRGSAMLTIPALHYGANRRNLIWLQ
                     VLERLDQAADGQ"
     gene            16327..17973
                     /locus_tag="PLJ64_01235"
     CDS             16327..17973
                     /locus_tag="PLJ64_01235"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HNC92146.1"
                     /translation="MKVAGKFAVALSVLLGVFVFTGSASATTFCVPGYFDGCPDNGSN
                     VVPSGAGAGEKLENALSQNGSDGSPDTVYLDSGTYVDTDSLKAGGSDGLTVIGNGRDR
                     SFITSSVAGNYYVVDLNFGNSRHITMKDLAIVIPEDFPNDNGPGSAVQSKGDDFDSVD
                     VINENNTGTVYLSSGFANVMGGGSFTDIRLFGRNGARFGSAINVACNYPGQVTINGAG
                     VSDSQYGVLAPCSTVPVSIDRATFSGADSALQPSNGAQLTASNVLIESGDLSPVMVYN
                     AKGAGTTTLDLDHATVVATGDASMPAIRANVANVVAATDPIQVNVSNSIILGFDDTWE
                     MIAPTDPAKGDVGLSVSYSDFSPVGVVTGDSSLDTATGNIFDSPGFTGITDYHLTPGA
                     PTVDAGDPSAAGPLFDLEGNGRPIDGDFDSVAVPDMGAYELNPPPPNCPVDPSACAPK
                     LRKVKFRYRFGKGGTLRYRVSIKAKVIAVFRPVPKKAKSKRMTVRILKQGKQGANVIK
                     LGKRLLKPGRYRLAIRALNKTGDSSNVVVRKVQVRKAGRR"
     gene            17977..19578
                     /locus_tag="PLJ64_01240"
     CDS             17977..19578
                     /locus_tag="PLJ64_01240"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HNC92147.1"
                     /translation="MRVIIRTALGAAAFVCCLALAGPAAANNFCVPTYSADCPASGTN
                     EAEPDLNAALSAYGSDGNPDRVIIAPGTQTSTGSFKAPGNDPLEIIGSGRDKTFLTSS
                     ATGNTYVLDLTSGSNRAVTVKNLAVVVPASFSTGAAISESADRFESVDLISRNDGSSG
                     VFNPTGGSSFSDVRAFGQNGAGFGGVFYQQSQCGTGPVSFERIHITDASSGLIWNCPE
                     ANPVTVDRARFTGVGAAISVSDGALVSVTNTLVESGEGAPIRAFSATNKVTTLNLDHL
                     TMVATGDPNQPAIRAQVNGVASPTKDIEISIGNSIISGFADSWSLEAPVDSVKGNVSL
                     DASFLASDSDGLTAGESFVTGANNLALGGEAIFAAADDFRLATGSVVIDAGDPAATLP
                     DHDLDGEPRPVDGNADLVPVGDLGAYEYQPTCEEDPAFCPVDATAPRISKVSFTSRPG
                     RGSSLRLKISEAATVKAVFTPLSKRGRPKRGKVKLSRKARAGSLTIKLGKRKLKPGRY
                     RLTLQAADKAGNKSKMITRKVRVKG"
     gene            19587..21167
                     /locus_tag="PLJ64_01245"
     CDS             19587..21167
                     /locus_tag="PLJ64_01245"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HNC92148.1"
                     /translation="MRPVRFGVAAGTVMLALLVFSAPASATTFCVPGFFDACPDNGTN
                     QARTDLGDAMTDDGSDGIPDKVMIDSGTITSTGSIVAGGIDPLEVTGAGRDKTFLTSS
                     STGNISVLRINNRKGTKMSDLTIVVPASFPDEFGNGSAAQIGDSELTRVDVEVRNSDS
                     SAFASMVGSNLIRDSHVYAAEGSKVRWAFVTGGAPGKSTIIGARVEDPVYGFVADNPD
                     TSIEVKASRVIGPTAYGAWAGSGGQISLENTLIATYGGGALTADPENGESDPTIVTAV
                     SSTMVNTGTSTSPGIQVDIPNSITAGDAFVTVTDSIIRGYGATWDLFVPIGPGFAAAN
                     LNVVYSNFPPVAAPGSAGNVNVGSPTNINQDPLFVSPQDFRLKPGSPSVDAGNPASTL
                     TTDIDGSPRPRDGDGNRTSINDQGAFEFQPTCATAPALCPTVSKVKFRFRAGKGGSLR
                     FLLSLAAKVRAVFTPVPAHRKPKRKVVKITRKLKAGQVKIKLGKHRLMPDRYKLKITA
                     YDKTGHSAKPLIKKVTVK"
     gene            complement(21194..21610)
                     /locus_tag="PLJ64_01250"
     CDS             complement(21194..21610)
                     /locus_tag="PLJ64_01250"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNC92149.1"
                     /translation="MTPANLIVDASIAVKWFRPREIEPERELAEILIAEASIRITSLC
                     LYEVGNVLATKTRDSDEQIGRKLEALVHYCGPPLELTVPDFRLAGNLARRHKLTFYDA
                     SYVAIAERLGRKVVSADQDLIGPGLAVDLATAVGAG"
     gene            complement(21610..21846)
                     /locus_tag="PLJ64_01255"
     CDS             complement(21610..21846)
                     /locus_tag="PLJ64_01255"
                     /inference="COORDINATES: protein motif:HMM:NF027249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HNC92150.1"
                     /translation="MAKVMVSIPDELLAQIDAEARRQGTTRSGLLQRAARVEVGVRQV
                     SRDEVRAQLDGLSSDWIDPGEDITEAIRRDRRNH"
     gene            22019..22321
                     /locus_tag="PLJ64_01260"
     CDS             22019..22321
                     /locus_tag="PLJ64_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92151.1"
                     /translation="MLCALTVRKVKPGTAEEFIENFRPPDDEYPGGWKRFFAIRNLAD
                     ENEVITFGLFDGDLDQLNASQAEDNKYEDRINAVAPFIESTGTSGVFEVLVDQSID"
     gene            22738..23655
                     /locus_tag="PLJ64_01265"
     CDS             22738..23655
                     /locus_tag="PLJ64_01265"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HNC92152.1"
                     /translation="MKLKTGVAALAVSMLLFTADAGAAVSHQVTGPEAKASRGFWTAA
                     RVAAALKRDPDSALNAASSPDRHPVATHHRPVTVYRPEVGKILAWDRNIGEFSCSGSI
                     IDTRSLRLVLTAGHCVYADGVWARRHIFIPNFKNGRRPFGAYRVKATWVSNAWFNNSF
                     GTRGMNFDIGLLVTRKRWNGSRIGENVGAIPIQTFPRRYGVTDIYGYPGGAMRTRVMR
                     TCRSHTKADWYGGRFFYGPTGLLARCNMAAGSSGGPWVSRYKAEGGGTIGVVDGLTST
                     GFSQGGRSYLTSPYFGRILVRLIKATEGR"
     gene            23748..23957
                     /locus_tag="PLJ64_01270"
     CDS             23748..23957
                     /locus_tag="PLJ64_01270"
                     /inference="COORDINATES: protein motif:HMM:NF021474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HNC92153.1"
                     /translation="MRTNIVIDDELMAEAQKLTGTKTKKETVDLALREMVAHRRRQGI
                     LELPGKFKWEGDLNEMRRNFFRKFD"
     gene            23954..24358
                     /locus_tag="PLJ64_01275"
     CDS             23954..24358
                     /locus_tag="PLJ64_01275"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HNC92154.1"
                     /translation="MIVADTSVWVDYLNGVWAPGSRELRRLLEEDETVCMTDHVLSEI
                     LQGYRREAEADEIASLLLTFPFLGLEGPGDYLAAASVYRKARSAGITLRSTVDLLIAI
                     PCIRREAFLLHNDRDFDQLARVSDLKIWQPAG"
     gene            complement(24401..25765)
                     /locus_tag="PLJ64_01280"
     CDS             complement(24401..25765)
                     /locus_tag="PLJ64_01280"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNC92155.1"
                     /translation="MPFAQAAKVPAGAGSGAVAPIAHKGRWFVDATGRVVQLRGVNEV
                     YKTAPYYPAADGFGADDVNLLSKLGLNVVRLGVDLRGLMPTPGKIETAYIDKLAQTVN
                     QLTKAGIYTQVDIHQDGYAPKYNGNGFPDWMAIDDGLPNPDLPFPVYYFGNTAMQRAW
                     DHFWDNSEVNGKGLQEYVIQSLTALVKKFRGNPHVVGYDVFNEPWPGTDFAPCAEATG
                     CPQLERELMMPFARKAVKATRKLTKTQPVFVEPFLFFNLGRPTVLPGSTGAWLSAHNY
                     ATDENDALMVKETVKAATRDQVPAILTEFGATSDNATIDRLTSIYDARIVSWIFWAYN
                     ENVVPDGSVTAGTGTATQDVIQALARPYPVAVTGTPTSLKFDPDTRVMDLTYGTAKRG
                     GGKFPAPLPSVVSVPRYTYPTGYKAIVKGARVTSAACSPKLRLQTRPGARKVSLTVKP
                     ASSC"
     gene            25977..26288
                     /locus_tag="PLJ64_01285"
     CDS             25977..26288
                     /locus_tag="PLJ64_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92156.1"
                     /translation="MDKGEPEGCLCVRARVREDLENLCQLESLTSYADTIEESRFSDY
                     RFRIHVKREDWVKAAAVLAEQIDYDNFKNAVKARQGRERAGVYTQVWNDLYGMQEPFA
                     R"
     regulatory      26302..26401
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            27073..27273
                     /locus_tag="PLJ64_01290"
     CDS             27073..27273
                     /locus_tag="PLJ64_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92157.1"
                     /translation="MSGGGWRYSSGIVSQFGRYCRDCLHEKGVKTTSYSAAIEKLPSD
                     KQVEVEKLRNKWTVEDARRKPE"
     gene            complement(27408..27632)
                     /locus_tag="PLJ64_01295"
     CDS             complement(27408..27632)
                     /locus_tag="PLJ64_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92158.1"
                     /translation="MPSRDEIAATIPRLGRGARINLKLKYGGEVLGTLYGLFDDQVFF
                     EEEEIAPIDLDRIDDLLVRVHTEQPGGPAS"
BASE COUNT         4702 a         9086 c         9046 g         4811 t
ORIGIN      
        1 cgatcgaggg ccggtcgatg tcgcatcccc aggcctcggc cacctcggcc acatcaagga
       61 agtgccggag cgtgcgaccc gacccgcagc cgaaatcgag cagccgtttg ccctcgtagt
      121 tccagtcttc cggcaaggcg gcgtcgatcg actgtcgggt catcaggccc agccactcga
      181 agtggtcgag cgcgttatcg gtctccggca gggagctgac ccggtcagcc aggtccaggg
      241 gcggaaacgg cggcttgtcc tgcgcgctca ccgaaagctc cgcgggcaga tgcctactcg
      301 tcgagcagcg actggacttc ctcgcgggtg aagagcggct tgccgcgctg cttcagcccg
      361 gagagcttct cggccgcggt gaaggcgctc gatccgagca gccgccgcag cagatcctcc
      421 tgtttggcga ccttctcttc cagcttgacc agctggcggg cccgggcctg gccggcgacc
      481 aggtgctcga cccggttgcc ttccatccgt tcgaggaccg ggtggcggtc atagggggcg
      541 atcacgcgct cgaccgcgct agcccaggga gcatcccggt gccacatcac gccgaagccg
      601 aagaaggccg ggactaccgc cagctggatg ccgggccgtt cggccaggaa gtcctcgatc
      661 gcggtcatcg tgccgttctt ctccccgcct tcctcggcgg cggcccagac gaagggcagg
      721 ccccattcgg cggtgccctt gatcccgggg gcgatgccct cgttgtgaac cagtggctgg
      781 cgctgatcct cggggatccg gtccggctcg tagtaggtgt cgcgccgggc gtgaggccag
      841 cagacatcgt ggaacatcag cagcggcatc ccggcgccgg gagcgcgctc gccgatgatc
      901 ttcagttcct cgctcagggt gtagtagttg tggtcgccgt cgatgatgat cgcgtcatcg
      961 aattcccgtt cgcgcagcac ctcctggccg gtctggcgca ggagttcaag ctccgggtgc
     1021 gccttcgcca gggcttcgag ctcgtccggc ggcagcgggt cgactcccgc gaccttgacc
     1081 ccgcgtgtct gcgcccattc gagcagctcg accgtcaact cgcccttgta ggagccgatt
     1141 tcgagcacgg ttttcgtttc gatcgcttcc aggcagcccg aaagcagctc gaagacgttg
     1201 ctcagcgagt agccccactg gagcggatcg gtaacgcgtt ttccgggagc catcggcggc
     1261 aagggtatag attcgcccgg cccatggatg acctcggtca gcacaatgac gccgctagcc
     1321 tgatggcgga cctgcgctcc cggcaccccc ggttcaccga agcggtgctg gccgacacgg
     1381 tggtgaccgc ccagcaccgg ggggagcgat acgagttccg cggcaagggg gatgccctgg
     1441 tcgcggccct tcgcctggcc tgggtgagcg acgccttcct ggcccaggtt ctctaccgcg
     1501 ccaaggccgc ctgccagcgg cgcgaagtcc gggttttgcc ccggctcttt caccggcttt
     1561 cgatgatgct ggcccaggtt tcgatcggcg acccggtggt gatgcacccc ggcgtctaca
     1621 tcgtccacgg ccaggtcgtg ctcgacggca tcgtcgaaat tcatcccggc gtggtgattt
     1681 caccctgggt cacgatcggc ctgcgggccg gcaacgtggt cggtcccaca gttcgcgagc
     1741 aggtgaacat cggtacgggc gccaagctga tcggaccaat cgacatcggg gctgcctcat
     1801 cgatcggagc caacgcggtc gtggtcgacg acgtcccggc cgggaccacc gcggtgggag
     1861 ttccggccag tcaacccggt tgaaaccggg catgcgggta agtactgaac ctgttctgct
     1921 ttgcaggccg ggctggtagc gtccagcggg ttcgagaaag cagaaccgaa ctcaccagct
     1981 aaaggcaggt ttttgatggg catttttcgt cgcaaggatc gtggagcagc cgcagtcgcc
     2041 gaggccgagc cggaactcaa tccggtggtc gaggaagccc gcgctctcga agctgacgga
     2101 aggatcttcg acgggatcga tctgctgacc cggaccaatc gcggaagcgg caacgtcgag
     2161 atcgagcggg agatcatcgc catgcgccac cgggccggcg cggaaatggt cgaagccggg
     2221 gatcagcacc cggtccaccc ggagcccgcc gactacgatt ttcgcctcga agacaaggtt
     2281 ccgaccgtca ccccggccga gctgaccccg gagattctcc gcgcggcaat cctcaagtac
     2341 ggctgcctga aggtaaccgg cgtgatggac gaagcgaccg cgaagtccct ggccgatgac
     2401 atcgatcacg ccttcgagat ccgccagcgc ctgagcgacg ccaaggaagt cgatccgacc
     2461 cggatctacg aggagttcgc ccccaacgag ccctaccagg tccagtacgt ggagcggcac
     2521 tgggtcgccc tggccggcgg ggtcctcgcg gccgactcgc cgaagatgtt cttcgagatg
     2581 gtcgactcgc tcgagcaggc cggggttcgc gacgtggtcg agggttacct cggcgagcgg
     2641 gccgccattt ccgcccagaa atgcaccctg cgcaaggccg atccgagcgt gaagggcggc
     2701 tggcatcagg acggcaagtt cctcggtgac gtccgtgccc tgaacgtgtg gctttcgctt
     2761 tcgcgctgcg gtgatgtcgc cccgagcatg gacatcgtcc cggtccggat cgacgagttc
     2821 gtcgcagccg gcgtcaacga aggtgacctc cactaccagg tgaccgacga gaccgcggcc
     2881 gaagcggcgg gtgaaaccgg catcgtccgg ccgatcttca accccggtga cgtgctcctc
     2941 ttcgacgaga tgttcctcca ccagaccggc tcggatcctt cgatgccgaa cccgcgttac
     3001 gcgatcgaga gctggttctt cggaccctcg gccttccccg aggagtacgc gccgatcgca
     3061 ttttgagccg ggcgggaggc cgttcccggc cacatccggc ccgagttccc aatagtctcg
     3121 tctgaatggt cgagcctgaa ttcgattccg agatcgagct cgggctggag gtaaagatcc
     3181 ccgggcagct gccggccggc cgggccgacg cgctcttcct ttacgggtac gcatttcacc
     3241 gccgccagga cgtcgagggc ctggagatcc tggtcgacgg ccagccgaac cgggcggtcg
     3301 ggcgcatgcc ccgccttgac ctcttcaagt cgcttcaccc gctgatcccg gagggcgaag
     3361 tctccgagac ccatgacgcc ggctcgctgg aggaccctga actgaagagc ttccgctccg
     3421 gtttctggtc cacggtcgag atccccgcca ccggaccggg caaggtcagg atcgaagtcg
     3481 ccctgaagct tgcggatggc agcatcagcc gcgcccccct cgccgagatc gagatggtcg
     3541 atcaggaaca acggttttcc acagatattg aggaagaccg tgggtcccgc cccccggagg
     3601 cacggatcgc gatctgcatg gcgacccaca acccggacat cgggctgctc aggatccagc
     3661 ttgattcgct gcgtgagcag acggtgaccg actgggtctg cctgatcagc gacgactgct
     3721 cgctcccgga gaccttccgc gagatcgagg cggagatcga aggcgacgac cgcttcctga
     3781 tctcccggtc cgaggcgagg ctgggcttct accgcaactt cgagcgggcg ctcggcatgg
     3841 tccccgagac agccgaactg gttgcgctct gtgatcagga tgaccgctgg tacccggaga
     3901 aactggccga gctggagcag gccctcgggg gcaagggcgc cggcaagtca ggccgccgcg
     3961 gcccacagct cgcctacagc gaccagaggc tggtcaccga agacggccag gtgatcgccg
     4021 agacctactg gtcgggccgg cgcaacaacc acaccaactt cgcctcgatg ctgatcgcca
     4081 acacggtgac cggtgccgcg tcgctcttca cccgggagat gctcgaatac gccctgcctt
     4141 tcccggaggc gccgggcgag cagtttcacg atcactggct ggccctggtc gcgatgtcca
     4201 ccggcggcat cgcctacgtg gacaaaccgc tctacgacta cgtccagcac ggctcggccg
     4261 tgctcggaca tgacgcggcc aacgccggga tcaaggcggg ccggccgaag cgcccgaagc
     4321 atctgagcat cggcgcggtc cggcagttct tctccggctg gcgttcggcc tacttcaacg
     4381 cctacctcag gctgtcggtc ctggcccggg tgatcctggc ccgctgcgac gggaagctga
     4441 gcggtcgcca gcgccgcacc ctgaagcgat acgtcaaggc ggagaagagc ccggccggtt
     4501 ttctctggct gctcttcagg ccggtccggg agctcttcgg ccggaatgaa accctcgggg
     4561 tcgagcggct gctgatccag ggaatcctct ggcgctacat catcttctgg agaaaccggg
     4621 ggctggagaa gccggtcggc tcgctctatg acgccagcct gcccccggcc ggccgggccg
     4681 ccaaggcccc ggcccacaac catccccaga ccgcccacat ccagcgcatg gtccggccgc
     4741 tggatctggc ggtcagcgaa gaggcgccgg agcgggtcaa tctgctgatc ccgacctttg
     4801 acctcaagca tctcttcggc ggctacatcg ccaagttcaa cctggcccgc aaactggccg
     4861 aggccggctt tcgcacccgc atcgtcaccg tcgacccgac cccgccgcta cccaggtcct
     4921 ggcgcgaaca ggtcgagtcc taccaggggc tcgaaggtgc attcgagaac atcgagatct
     4981 tcttcgcccg cgactccggc gaggcgctcg agataaatcc ccgggaccgc ttcgtcgcga
     5041 ccacctggtg gaccgcccac ctcgccaacg acgcgctgtc gcggaccgag cacgatcact
     5101 ttctttacct gatccaggaa tacgaaccgc tgaccttcgt gaccggctcc tggtacgcga
     5161 tggcggatgc gacctacgac ttcccgcacc gggcactctt ttcgaccgag ctgctcaggg
     5221 agttcttccg cgaccgcggc tacggggtct atcgggaggg agcggccacc ggggacgagc
     5281 tttcggtctc cttccagaac gcgatcacgc cggtcagtgc cccgcccgcg gccgaactct
     5341 cggcccgggg cacccgtcgg ctgcttttct acgccagacc cgaggcccac gcggcgcgca
     5401 acatgttcga gatcggcctg atgagcctgc agcaggcggt cgaagacgga gtcttcggcc
     5461 cggaatggag ctttcacggg atcggcacgg ttgagggcca gagccgcctc aggctgaccg
     5521 acgaggtcgc cctcgagatc ctgccccgaa ccagccaggc cgactacggc gagctgctgg
     5581 ccggccatga tctcggcctt tcgctgatgc tgaccccgca tccgagcctg cctccgctgg
     5641 agatggcttc ggccgggatg ctgaccgtta ccaacacctg cgagaacaag accgcggaca
     5701 agctgaccgc gatctccccg aacctgatcg ccgcaccgcc caccgtgccg ggcgtggttg
     5761 cggcgctggc cgaggcggct gccgcggccg gtgacgggga gcggcgcgcg gccggttcgt
     5821 ccgtagactg gagcagtgac tgggagacct cgcttggtcc ggatgtgatg gaccgggtga
     5881 tcgggttcct ccggtgactc ccgattccat gaccacggat ccgctcgccg agaccaagac
     5941 cagggacatc accgaaaacc cggtggtgat ctccgtcgag ggcctgaaga agagcttcaa
     6001 aatccccctg caccgggttg agtcattcaa ggagcgggcc gtccatccgc tcagaaagac
     6061 cgagtaccgg gagctgaagg cgctcgacgg gatcgacttc gaagtcaggc agggagagtt
     6121 cttcgggatc gtcggtcgca acgggtcggg caagtcgacc ctgctgaaga tcctcgcctc
     6181 gatctaccgg gctgacgcgg gaaaggtccg gatggccggc cggctcgccc ccttcatcga
     6241 gctcggggtc ggtttcaacc acgatctcac cgcccacgac aacgtgatca tgaacggcgt
     6301 gatgatgggc ctcagccggc aggaggccgc aagccgcctc gaccgggtgg tcgagttcgc
     6361 cgagctcgag gatttcaagg agctcaaact caagaactac tcctccggga tgctggtccg
     6421 gctcgccttc tcgctgatga tccaggccga tgccgacatc atgctgatcg acgaggtgct
     6481 ggcggtcggt gacgcctcct tccagcagaa atgcgccgat gtcttcaacc agatgcgcga
     6541 cgccggcaag acgatcgtcc tggtcaccca tgacatggcg gcggtcgaat ccttctgcga
     6601 ccgggcgatg ttgatcgagg gcggccacgt gatcgaggcc ggcgaacccc gcgatgtcgc
     6661 ccgggcctac ctgaagctga acttcgacgc cgaaacgccg cggcaggaaa ccctcggctt
     6721 ccttgaggat ggcgaggtcg aggatgactt catccccggg gtcgacatcc aggcgaagct
     6781 gatcgacgcc tggctcgacg acggcgccgg cgagcgggtg accaacgtcg agatcggtga
     6841 tccgctgcgg ctcaacctgg agatcgaggc cctccgtgac ctcgaacggc cgcttttcac
     6901 cttccagatc aacgacgcca agcacaccca ggtcaccggc ttcaaccaga ccctcgaggt
     6961 ggagggcccc ggcgacgtga tccacgaggg ccagaaggtg acgatcagcg gcgagatcgt
     7021 caaccggctc acccccggcc gctacctgct gacctgctgg gtcacccgca accgggagac
     7081 ttcggagcgc gtggtccagg ggatcaagct cttcgagttc gtgatcttcg ggatgaagcg
     7141 cacccccgga ctggtcgagg cggaagccag cctcaacgcg aaggtggcgg gcagcaatgg
     7201 ctgagcaaac ggcgactgcg gcggcatccc ccgaagaggc gatggtctcc agtccggatt
     7261 cacccgaact gcgggaagtg accggcccca ccgcgctcgg tggcggctgg cgccgctcgc
     7321 tggatttgct ctacctgatc tcgatcaacg acttcaagaa gacctacttc gacacggtgc
     7381 tgggatacgt ctggtcgctg tttcggccgc tgatcctctt cgcggtgctg ctcttcgtct
     7441 tcacccagat cttccggatc ggcgatgacg tgccgcatta cccggtgatg ctgctcttca
     7501 acatcgtcgt cttcaccttc ttccaggagg cgacgatcgc cgcggtgacc tcagtcgtgg
     7561 cccaggaagg ggtggtgcga aagacccagt tcccgcgggt ggtgatcccg ctcgcggtgg
     7621 tgctgaccgc gctgttcaac ttcggcgtga acctggtcgt ggtcctggtc ttcctgctcg
     7681 cctccggggt tccggtcgag tggacctggc tgttcttccc gctcctggtt ctcgtcctca
     7741 cggtgatcac gaccgcggtc tgcctgctgg tctcctcgct tttcgtcaga ttccgggacg
     7801 tcgggatcat ctggggcgtg ctggccacgg cgctctttta tgcgaccccg gtcctctacc
     7861 cgatcgaggt ggtgccggag cgcttccacg acatcgtgct gatcaacccg ctcaccccga
     7921 tcttcatcga gctgcggcac ctggtgatcg acaactccgc cccgaccgcg gtcagcgccg
     7981 ccggcggatg gctcggcctg ctccccgcgg tcgccgtcac catcgccctc tgcctgctcg
     8041 cggtcaaagt cttcagccgc gaagctcccc gcatcgccga agaactctag gtctgcgagc
     8101 ttcgcccggc ctacctcttc ttcagggcga tcgggacctg gtacatgtcg ccggtgagga
     8161 atccggtttc gacgatctcg aaaaggccgg tccagttctc ttcgatgtag gggaggctga
     8221 tcaccgtgtc gccgtaggtc atctcgccct cgtcgtattg agggtgacca tcctcgaccg
     8281 ggtggggcac gaagatgaac ctcggctcct tcatttcccg gctggcgaaa tcatccgaaa
     8341 gctccgcccg ggccgcttcc attagcgggt cgtggtccag gtaggccggc ggacgcaccg
     8401 tgatcacggc gaggccaccg ggcgccaggg cggcgtggat cgccttgagg caggcctcgt
     8461 gagccgcttc ggaaatgtgg gtgaagaccg agaagctgaa ggccaggtcg aggttctcga
     8521 acggaagccg ctcgggcagg aaaccggtct gccggagttc gccgggcacg ccggtctcgg
     8581 cgcagacttc gaggatctcg ggggtggggt cgcagccgaa gagccggtcc ggcattgcgt
     8641 cccgggcgaa gaagcgggtc agccggcccc agccgcagcc gaagtcgagg atccgggccc
     8701 cgtcgagcgg ggcccggccg tgccgctcgt tcagctcgcg aaccctggcg taaaagttct
     8761 tgctctggcc cagcagctcg aggccggtcg cgccgttcca catccactga aggccgggtt
     8821 ccggggcgcc gggcaggagc gctttgatgt tcgggtagag gtcgtactcg cgggtcagca
     8881 ggacggccca gaggtcatcg ccgagcccgc ggaacagttg ccacgcctcc tccggcgggg
     8941 tctcggcgca ggcagcgtcg atcctttcaa gttcctcgcc gaagaaggtg tcgagccagg
     9001 aggtccagtc tggttcgacc ttctcctcga ccatcacctc ggcccaggga atctccgttt
     9061 cgcggccgcg cagccgccgg gcggcatcgc tgatcgaccg gagcggacgg gtcacccgcc
     9121 agctcagggt tgactcgtag ccctcccgga ccgcgtcgat cttctcgtca gtgctcttcc
     9181 cctgttcggc cacctgaggg gcaaccctag aggttgtcta gtcgagcagg cgcccgatcc
     9241 gttccatcgt tgcgtcgtcc agggcctggt cccagttgtc gggccacccc acctttgagc
     9301 cttcgacccg ggcctcgaag tcccggctca tcacttcggc cgaggccagc gcttcctcga
     9361 tctggtcgat ccgcggctcg gcggcgatca ggttgcccga aatcgcctcg agtgaagccc
     9421 ggtccttgtt cgcgtaggtg ttggtgacgg tgacgagccc ggcggccgcc atctcgaggg
     9481 ggaccaggct cggatgcggg gtgtacatca gggccaggcc gacgtcgaac tggcgtagcg
     9541 tgtccgcgta ctcccgctgg gcgacccgcg gcaacatccg cagccgggcc ttcgactgcg
     9601 gcagggtcag ctccccggtc ccgtccacgt tcccgatccc ggccaggtcc cagccccgga
     9661 aatggccagc gagaatcgcc cggtcgaggg ccatgatccc cagctcgaag aggttgcgtt
     9721 cggcgtgggc ctcggggcgt gcgtagaaca ggagccggcg ccggtccttg ccctgaagct
     9781 ccccagtcgt gaccgtggcc gccggggtga tcgcattgtc gaagaccgcg gcgcccgctt
     9841 cccccggtcc ttccggccgg tacacgccaa ttccctggtc gcggaagtag tcccgcagga
     9901 acccggtcga gaagatcgcg ttgtggtcta gttcgtagct ctgatcggcg agggcggcgg
     9961 cgctgcccat cggaaagatc agcggctcgt attcctggat caggtaagtg aaggactcct
    10021 gatcgagttc cgcggcagca gccgaggcga tgtgggcggc agtccagtgc gtggcgatca
    10081 ggcggtcatc cggattcatc gcgaccggga cggcccgatc gctgccatcg atcacttcca
    10141 ggtcacccac cgagtcaccg atcccctgat aggcggcgac ctgccggcgc cagtcatcgg
    10201 gcaggtcgag tggctccagg gtgacgatcc ggacccggtg gccgccctcg cccagccgcc
    10261 gggccaggtt gaagaccgcg atgaagccac cgaagaaatg tttgaggtcg acggcggggt
    10321 ggatgatctc gacccgcaaa ggctggccgg ggtcggccgc gacgttcagc ggttccagtt
    10381 tctcggtgat ccagccggcc ttctggttgc cgcgcagctg catcgctccc cccagcacgg
    10441 cccggccggt caggatgccg ccgccgatcg ccaccggaat cgccaggagg atgaaggtcg
    10501 cgccggccga gaggctctgg ccaatcaccc cgaggatcac gccgatcaga aaggccccgg
    10561 caaccggcac gccgatcagc ccggccagcc tcgccttgcg tccggcggtg ccttcatcgg
    10621 ggttgccaaa agtccgtcgg atcagctccc gggggtgcgt cacgcgacgc agcgtattac
    10681 cttgacgggc aagcaatgga cctgtccctg atcattcccg cctacaacga gagcgaagcc
    10741 ctgcccgggc tgatcgccga gatcgactcc gcctgcgaag ggctcggcct tcagtgggag
    10801 acgatcgtta tcgatgacgg ctcgaccgat ggcaccttcg gggtgatctc ggagatcgcg
    10861 aagtccaatc cccgggtcgg ggtcgtcagg ctgaggcgga acttcggcaa gtcggtggcc
    10921 ctggcggtcg gcttcgatgt gacccgcggc gagaaggtga tcacgatcga cggtgatggc
    10981 caggatgacc cggccgacat cccgcgactg ctcgaaggcc tggaaaacgg gtccgacctg
    11041 gtctcgggct ggaagagcga ccgccaggac cccgtcaccc gtcgcctggc atcgaggatc
    11101 ttcaactggt tcaccgcccg ggttaccgga gtcgacctgc atgacatgaa ctgcggcttc
    11161 aaggcctacc gcggcgaatg cgcccgctcg atcgaggtct acggggagat gcaccgcttc
    11221 ctgccggcct tcgccgccca gcagggctgg cgggtaaccg agctgccggt gaatcaccgt
    11281 ccccgcctcc acggctcgtc ccgcttcggc ctcgagcgct accggcgcgg cgccctcgac
    11341 ctgctgaccg tcgcctttct cggccgctac cagaaccgtc ccctgcacct tttcggcctg
    11401 ctcgggctcg tcctcggctc gcttggtttt ctgatctgcc tctacctctc gatcatctgg
    11461 atcggtggcg aggcgatcgg cgagcgaccg ctgctcttcc tcggggtgct gctgatcgtg
    11521 gtcggggtcc agctgttttc actgggcctg ctcggccaga tgatggtggt cggccggcgg
    11581 gccggtgtct ccgaggagaa ggtgctgatc gagaaggtgc tcagccccga aagcgatccg
    11641 gaactcggtc tcagccggct ggaagcggtc ggcagcgggc gatgatccag ggcatcgagg
    11701 tctgaacctc gaccacttcg gtccgggtcg aaaggaaggc cttcaagctc ttcggattcc
    11761 agtggttcac atgctcggga tggttgcccc aggtcttgag gtacttgccg cgggcgaagc
    11821 tgccgagccg gaaccagggc tcatgtggca ccgaaacgac gatgtcccgg ccggaaaccc
    11881 gcgccagttc ggccaggccg gcggccgggt cgggcaggtg ttcgagcacc tcgaggcaga
    11941 agacgaggtc gaagctgccg tcctcgaacg gcaggttgag caggtcggcc tgccggatgg
    12001 tcgcgccggg gagccgccgg gcggcgaact cgaccgactc ggggttgagg tcgatcccgg
    12061 tgggggcggg cggcagaacc tcgctcaggc gatccagggt ttccccttcg ccgcatccgg
    12121 catcgagcac ggtggccggc ttcagcggct tcacgacttc ctcgaccgtg tcgtagaagc
    12181 cgccgatcag ccgcttcacc accgggttcc cggtctggaa cttctcgtag ttggtggtgt
    12241 cgacggtttc ggtgctgttc gctgccatgg cgggtgatcc tatgggcgga ggctctcgat
    12301 gaggtccgtg aggctgcgct caagcgtgat cccggggcgc cagccggtca gctcgcgaag
    12361 cttcgagaag tccccgcgga tcgaaagcag cccggacgcg ggccgtccgg ttccggagcc
    12421 gcgcagggtc aggtcaaccc cggtcagctc gccggtcagg gcggccagtt ccgccaggct
    12481 cgtcgccttt cccgagcaga ggttgagggt gccggttgct tcttcctcga gaacccggcg
    12541 gaccgcccgg gcggcgtcgc gcacgtcggt gaagtccctg gcggtggccg gctccccgac
    12601 ctccacctcg gcccgtccgc cagtcccggc ggccagggcg gcccggacga actctgaact
    12661 cgcctgggtt gaaggctcgc cggggccgat caggttgaag cagcgcagca ccgtcaccgg
    12721 tatcccgtag ccgcgtcggt actggccgca gatcgtctcc atggccagct tcgaagcggc
    12781 gtagggcgtg gtcgccgcgg tcgctgaaga ttcgtcgaag ggcgctgacc cggcgtagac
    12841 cgcggccgag gaaagggaca cgagctgcgc cccgggggcc cgttgccgca gcgcttcaag
    12901 caggttcagc gccccgcccg cattgaccgc gaagcaggcg gaagggtttg cccgggccga
    12961 tgccggtgag gtcatccccg cggcgttgac cacggccgcc ggtgcggtgg cagcgaccag
    13021 ctcgctgagg ccggcgggat cgagcagctc ggattcgctc cgctccggac cgagcacctc
    13081 gattccggcc gcggcgagtt cggccgcaat ccggctcccg aaaaagccgg aagccccgag
    13141 cacgagaact ctcccgggcc cggtcacggt cctggaattc agccggtctt gccgatcagc
    13201 cggtcgcggc ccgactgttc gatcgtcttc ttgaggccct cggacagggg cacggtcggc
    13261 tcccagccga gcagctcctt cgcccggtcg atgttgggct ggcggacctg cgggtcatcg
    13321 gtcggaagcg gctcgaaggt gatcgtcgat tcggccccgg aagcctcgat caccgcgttc
    13381 gcgagctgca gcagcgtgta ctcgtcgggg ttgccgatgt tgaccggcag gtgctcgccc
    13441 gattcggcca gcgcgatcag gccgcggacc aggtcatcga cgaaacagaa ggaccgggtc
    13501 tgcgaaccat cgccgaaaac ggtcagcggc tggttctgca gggcctgccg catgaaggtc
    13561 gggatcgccc gcccgtcatg ggcgcgcatc cgtgagccgt aggtgttgaa gatccgcacg
    13621 atcgaggtgt cgacgccctg ctggcggtgg taggccatgg tcagggcttc ggcgtaccgc
    13681 ttcgcttcgt cgtagacccc gcgcgggccg atcgggttga cgtggcccca gtagctctct
    13741 tcctgggggt ggacctgggg gtcgccgtaa acctcggagg tcgaggcgag caggaagcgg
    13801 gcccgcttga acttggccac gccgagcatg tgccaggtgc cctgggcgcc gaccttcagg
    13861 gtgtgaagcg gcagccgcgc ataatcgatc gggctggccg gggaggccag gtggtagacg
    13921 aagtcaacct cgccgtcgag gtggacttcc tcggtgatgt cctgcggcag gaaggtgaaa
    13981 tcgggattcg tgatgtgctc gatgttctgg agtgatcctg tgtccaggtt gtcgatgcag
    14041 atcaccttgt tgcccttttc gagcagggca tcgcaaagat gggatccgac aaaaccggct
    14101 ccgccggtaa ctacgctgac tgtcatgggc ggatcgtatt caaggttcgg ccgacgatgc
    14161 cgtcctgatc gtctaccgtt tactgcctgt cacttcgggg ggattggaag ccatgagcgc
    14221 cgtcaggcga aaagcaatcg gggtcctgct gcttgccggc cttgtcctct tctgcctgcc
    14281 agccgcctcg ggagcggtcc cggcctggca gtcggccccg gttatcccct ctctttcgaa
    14341 cggggatcag gccaggctga aggaccgggt cgcggccggc gagcggaccg ggttgcgcag
    14401 cggcgtcttc gccaaggtcg gggattcgaa caccgagttc tcccccgacc tctacggcct
    14461 cgcctgccgg aagccgaccg gtctgagcgc cgcccttcgc ggcaccctcg ccacctacag
    14521 ccgcgtgcgc ctgccgaacg ccagggcgct gccgggctgc cggccgtgga cctcgttctc
    14581 ccgccgctcc tcggccgccc aggccggcgt gcactcgacc tggcctctaa ccccggtcaa
    14641 ggatcttccc gacgagagct actgggccaa gccgcccggc tgcccgctca aaggcactcc
    14701 gctgagctgc gagatcgacg cgaccatgcc ccgctacgcc atggtcatgc tgggcacgaa
    14761 tgacatcggc atggacatcg ctttcggcat ccagcctggc agcaggatcg tcagccgcct
    14821 cggcggcgtg gtcagggccc tgctcgcccg gaaagtggtt cccgtgctga gcacgatccc
    14881 gccgatcatc cggccggacg gcaccaggca aggcgaggtc acggccggtg tggcccggag
    14941 caacgccggc atcttcgaac tcgcccggaa gtggcacctg ccgatgatca atctctggag
    15001 ggccctgaag gagccttcga tgatcgacct cggcctggcc ggggacggcc tccacctcag
    15061 cgtcgcgggg gcaggcgggg agatggtcgg tgtccagccg ggagcttcga ccttcgccga
    15121 ctcggttgat ttcactgcgg ccggcctgcg ctacggggca aaccgccgca acctgatctg
    15181 gctgaagacc ctggcccggc tcgaccgggt caccggttga catgggccgc cgcttcacag
    15241 ccgtcctcct ggccgggctg atcctgctgg ccggttgcgg ctcctccggt gaggattccg
    15301 ggcaagctcc cgacccctcc cgggcacctg cccgggccgg ggaatcggcg cggaccccgc
    15361 aaccggtggc cgacctttcg tccggctcgg tgctcccgga cctctcgggg gcggccggtg
    15421 accgcctgcg ccgggaggtt gcccgggccg acgaacggaa gctgaacccg gatgtcttcg
    15481 ccaaggtcgg cgattcgaac accgagtggc cgcagaacct ttacgggctc ggttgccgcg
    15541 aggtcggtta cggctcgaac ggccagctcg aagcggtggt gaagaagtac tcgaaggtga
    15601 ggtttccggg cctcgtctcg ctggacggct gctcaccggt caattcgctt acccgccgtt
    15661 cggcggccgc ggtttccggg gtctggaccg agtggctgat gaccccgacc gaggatctct
    15721 acgcgaccga ggtcggccct ccgaccgacg agtgccggcc ggagcagact ccgctcgact
    15781 gcgagatcga actgatccgg ccccgttggg cgctgatcat gagcggcacc aatgacgccc
    15841 tgctcggcaa gccgctcggc gacacctacc gcggcctcct tacgaagatg gtcagccggg
    15901 tccgcgagct cggttcggtg ccggtgcttt cgaccctgcc gccgatggcg gtcctgaccg
    15961 agaacggcca gcccggcgag gagaggatcg ccgaagcgaa ccggatcatt cgcgaggttg
    16021 cgtcgacgga gcaggttccg ctgatcgacc tgcactcggc cctcgtcgct cccgggatgg
    16081 tcaaccaggg gctcggcccg gacggcctcc acctcggggt ttacggcggg gaggctcagc
    16141 cggacgtgat gcgcggctcg gccatgctga cgatccccgc cctccattac ggcgcgaacc
    16201 gccgcaacct gatctggctg caggtgttgg agcgtctgga tcaggccgcc gacgggcagt
    16261 aagcttccgc gccggccttt tccgccagtc aacgaagccg gcctgtcaag aaaaggggag
    16321 ttctgaatga aggttgcggg gaagttcgca gttgcgctgt cggtcctgct cggggttttc
    16381 gtcttcaccg gttcggcatc ggcaaccacc ttctgcgtgc ccggctactt cgacggctgc
    16441 cccgacaacg gctcgaacgt ggtcccctcc ggcgccgggg ccggtgaaaa gctcgagaat
    16501 gcgttgtccc agaacggcag cgacggcagt ccggacacgg tctacctgga cagcgggacc
    16561 tacgtcgaca ccgattccct gaaggctggc ggcagcgacg gtttgaccgt gatcggcaac
    16621 ggccgcgaca ggagcttcat caccagttcg gtcgccggca attactacgt ggttgacctc
    16681 aacttcggca actcccgtca catcacgatg aaggatctgg cgatcgtgat ccccgaggac
    16741 ttcccgaatg acaacgggcc cggctcggcg gtccagtcca agggtgacga cttcgattca
    16801 gttgacgtga tcaacgagaa caacaccgga acggtttatc tctcgtccgg atttgcgaac
    16861 gtgatgggcg ggggcagctt caccgacatc cggctattcg ggagaaacgg cgcccggttc
    16921 ggcagcgcga tcaatgtcgc ctgcaactat ccggggcagg tcacgatcaa cggcgccggg
    16981 gtctccgaca gccagtacgg cgtcctggct ccctgctcca cggtccccgt ttcgatcgac
    17041 cgggccacct tctccggggc ggattcggcg ctgcagccgt ccaacggtgc gcagctcacc
    17101 gcctcgaacg tgctgatcga aagtggcgac ctctccccgg tcatggtcta caacgccaag
    17161 ggcgccggca cgactacgct cgacctcgat cacgcaaccg tggtcgcaac gggggatgcg
    17221 tcgatgccgg cgatccgggc caacgtggcc aatgtcgttg ccgccacgga cccgattcag
    17281 gtcaacgtga gcaactcgat aattctcggc ttcgacgaca cctgggagat gatcgccccg
    17341 accgatccgg ccaaaggcga cgtcggcctt tcggtcagtt actccgactt ttccccggtc
    17401 ggcgtggtga ccggcgattc aagccttgac accgcgaccg gcaacatctt cgacagcccc
    17461 gggttcaccg ggatcaccga ctaccacctg acccccggcg cgccgaccgt tgacgccggg
    17521 gatccttccg ccgccgggcc gctcttcgac ctcgaaggga acggccgccc gatcgacggt
    17581 gactttgaca gcgtcgcggt cccggacatg ggcgcctacg aactcaaccc gcctcccccg
    17641 aactgcccgg tcgacccgtc cgcctgcgcc ccgaaactcc ggaaggtcaa gttccgctac
    17701 cggttcggca agggcgggac cctgcggtac cgggtctcga tcaaggcgaa ggtgatcgcc
    17761 gtcttcaggc cggtcccgaa gaaggcaaag tcgaagcgta tgacggtccg catcctcaag
    17821 cagggaaagc agggcgccaa cgtgatcaag ctcggcaaac gcctgctgaa gccgggccgc
    17881 taccggctcg cgatccgcgc tctcaacaag acgggcgact catccaacgt ggtggttcgc
    17941 aaggtgcagg tcaggaaagc cgggaggcgg tagacgatgc gcgtgatcat tcggaccgcc
    18001 ctcggggcgg cggcgtttgt ctgttgcctg gccctggccg ggccggcggc ggccaacaac
    18061 ttctgcgtcc ccacctactc ggcggattgc ccggccagcg ggaccaacga agccgaaccg
    18121 gacctgaacg cggcactctc ggcctacggg tcggatggca accccgacag ggtgatcatc
    18181 gctcccggca cgcagacttc caccggcagc ttcaaggcgc ccggcaacga tccgctggag
    18241 atcatcggct cgggccgcga caagaccttc ctgaccagca gcgcaaccgg caacacctac
    18301 gtgctcgacc tgacttccgg cagcaaccgg gcggtcacgg tgaagaacct ggccgtcgtg
    18361 gttccggcca gcttctcgac cggcgcggcg atctcggagt ccgccgaccg gtttgaatcg
    18421 gttgacctga tcagcaggaa cgacggttcc tccggggtct tcaacccgac cggcggcagc
    18481 agcttctccg acgtccgcgc ctttggccaa aacggggccg gctttggcgg ggtcttctac
    18541 cagcaaagtc aatgcggcac cggcccggtt tccttcgagc ggatccacat caccgacgct
    18601 tcgtcgggcc tgatctggaa ctgccccgag gcgaatccgg tcacggttga ccgggcccgg
    18661 ttcaccgggg tcggagccgc gatcagcgtt tccgacggcg ccctcgtttc ggtcaccaac
    18721 accctggtgg aaagtggcga gggagcgccg attcgcgcct tcagcgccac caacaaggtg
    18781 accacgctca acctcgacca tctcaccatg gtcgctaccg gcgaccccaa tcagccggcg
    18841 atccgcgccc aggtgaacgg tgtcgccagt ccgaccaagg acatcgagat cagcatcggc
    18901 aactcgatca tcagcggctt cgccgactcc tggtcactgg aagcaccggt tgattccgtg
    18961 aagggcaatg tgagcctgga cgccagtttc ctggcctccg attcggacgg gcttaccgcc
    19021 ggcgagtcct tcgtgaccgg ggcgaacaac ctcgctctcg gtggcgaggc gatcttcgcg
    19081 gcagccgacg atttccgcct ggcgaccggt tccgtcgtga tcgacgccgg cgaccctgcg
    19141 gccaccctgc cggatcacga cctcgacggc gaaccccggc cggttgacgg caacgccgac
    19201 ctggtcccgg tcggtgacct cggggcctac gagtaccagc cgacctgcga agaggacccg
    19261 gcattctgcc cggtcgatgc gaccgccccg aggatcagca aagtgagctt tacctccagg
    19321 ccgggccgcg gcagttcgct tcggctcaag atctccgagg cggccacggt caaggccgtg
    19381 ttcacgccgc tttcgaaacg gggcaggcct aagcgcggaa aggtcaagct cagccgcaag
    19441 gccagggcgg gctccttgac gatcaagctc gggaagagga aactcaagcc cggccggtac
    19501 aggctgacgc ttcaggcagc cgacaaggcc ggcaacaaat cgaaaatgat cacccggaag
    19561 gtccgggtca agggataggg gcagacatga ggccagtcag gtttggagtc gccgcgggaa
    19621 ccgtgatgct cgcgctgctc gttttctccg ctccggcttc ggccacgacc ttctgcgtgc
    19681 cgggcttttt cgatgcctgt cccgacaacg gcaccaacca ggcccggacg gacctgggcg
    19741 atgcgatgac cgatgacggc agcgatggaa tcccggacaa ggtgatgatc gactcgggga
    19801 cgatcacctc caccggctcg atcgtcgccg gcggcatcga tccgcttgag gtgaccgggg
    19861 cgggcaggga caagaccttc ctcacctcga gctcgaccgg gaacatcagc gtgttgcgga
    19921 tcaacaaccg caagggcacg aagatgtccg atctgaccat cgtcgtaccg gcttcgttcc
    19981 ctgacgaatt tggaaacggt tcggccgccc agatcgggga ttcggagctg accagggttg
    20041 acgtggaggt caggaactcc gattcgagcg ccttcgcctc gatggtcggg agcaacctga
    20101 tcagggattc acatgtgtac gcggccgaag ggtcgaaggt gcgatgggca ttcgtcaccg
    20161 gcggggcacc gggcaagtca acgatcatcg gagccagggt tgaagacccg gtctacgggt
    20221 tcgtagccga caaccccgat acctcgatcg aggtgaaggc aagccgggtg atcggcccga
    20281 ccgcttatgg cgcctgggcc gggtcgggag ggcaaatcag tctcgagaac accctgatcg
    20341 cgacctacgg gggaggcgct ttgacggcgg acccggagaa cggcgaatcc gacccgacca
    20401 tcgtgaccgc ggtctcgtcg acgatggtca acaccggcac ctcgacatct cccgggattc
    20461 aagtcgacat tccgaattcg attaccgccg gtgacgcctt cgtgacagtc accgactcga
    20521 tcatccgtgg ctacggggcg acctgggatc tgttcgtccc gatcgggccg ggctttgccg
    20581 cggcgaacct caacgtcgtc tattcgaact tccccccggt cgccgccccg ggctcggccg
    20641 gaaacgtaaa cgtcgggagc ccgaccaaca tcaaccagga tccgcttttc gtcagccccc
    20701 aggactttcg cctgaagccg ggctcacctt cggttgatgc gggcaatccg gcctcgaccc
    20761 tgaccaccga catcgacggc agcccccgcc cccgggacgg tgacggcaac cggacctcga
    20821 tcaacgatca aggagctttc gagtttcagc ccacctgcgc gacggccccg gcactttgcc
    20881 cgaccgtctc gaaggtgaag ttcaggttcc gggccggcaa gggcggctcg ctccgcttcc
    20941 tcctctccct ggcggccaag gtgagggcgg tcttcacgcc ggtccccgcg caccggaagc
    21001 cgaagcgcaa ggtggtgaag atcacccgca agctcaaggc cggccaggtg aagatcaagc
    21061 tcggcaagca ccggctcatg ccggaccgct acaagctgaa gatcaccgcc tacgacaaaa
    21121 ccggccacag cgccaagccc ctgatcaaga aggtcaccgt caagtaatcc cgcgcccggg
    21181 ccgggagcgt tcttcagcca gccccgaccg cagtagccag gtcaaccgcc agccctggcc
    21241 cgatcaggtc ctggtcggca gaaaccacct tccgtcccag ccgttcggcg atcgccacat
    21301 acgaagcgtc atagaaggtg agcttgtggc gtcgggccag gttgccggcg aggcggaagt
    21361 cggggacagt cagctcgagg ggagggccgc agtagtggac gagcgcctcc agtttgcggc
    21421 cgatctgttc gtcgctgtca cgcgtcttgg tcgcgagcac gttcccgacc tcgtacaggc
    21481 atagcgaggt gatcctgatc gacgcctcgg cgatgaggat ctcggccagc tcccgttccg
    21541 gttcgatctc cctgggccgg aaccacttga ccgcgatgct tgcatcgacg atcaggtttg
    21601 ccggagtcat caatggttcc ggcgatcccg ccggatcgct tcggttatgt cctccccggg
    21661 gtcgatccag tccgaggaaa gcccgtccaa ctgggctcga acttcgtcac ggctcacttg
    21721 ccggactccg acctcgaccc tcgcagctcg ctggagcagc ccgcttctcg tcgtgccctg
    21781 gcgccttgct tcggcgtcga tctgggcgag caattcatcg ggtatggaga ccataacctt
    21841 cgccatcctt ccgagtatat cccgttggca atacccactt ctgtctttcg aacatgccac
    21901 acccgcaagc ggaatcggct tcagggccac gaatcgtcac ggttctgtca cagagatttt
    21961 ctcgttctgt tgcacagttg cggttaggct cggtgcggag gcagagaaag gagcagtcat
    22021 gctgtgtgca ttgacggtcc gcaaggtgaa gccgggcact gccgaggagt tcatcgagaa
    22081 cttccgcccg cccgacgacg aataccccgg cggctggaag cggttctttg cgatccgcaa
    22141 cctggccgac gagaacgagg taatcacctt cgggctcttc gacggtgacc tcgaccagct
    22201 gaatgccagt caggccgagg acaacaagta cgaagacagg atcaacgcgg tggccccgtt
    22261 catcgaatcg acaggaacca gcggcgtatt cgaggttctg gtcgatcagt caatcgactg
    22321 aatcgcggcg gcatccccgg cggcccggtc gggcgccccg cgaggggcgc gccggccagc
    22381 ttcagagatg ccgccgcccg cggcgcccaa catctgggtc ggacgccggc cagtcgaagc
    22441 tcagtcggag ttcatccaga tcaaccagtg gaccgggctc acggttccgg taggtggcca
    22501 gctcgcttcg cacggccgac tggaacattt cgtcccggtc aaccccgcgg atcgcggctt
    22561 gcctctccat tcctgtgagc agtccgtccg ggatctgtcc ctccgtcctt gccattggcc
    22621 gaacatagcg ccggtccttc ggcctgtgtg agatatgtca accccgggtc gatgaaaaac
    22681 ccggcatttg cggaattcct ttgtcagatc agggagaatg gaagcgacat ggggggcatg
    22741 aaactgaaaa cgggagtcgc agctctcgct gtctcgatgc ttctgttcac cgccgatgcc
    22801 ggcgcagcgg tcagccacca ggtgaccggg ccggaggcga aggcatcccg gggtttctgg
    22861 acagcggccc gggtggctgc cgcgctcaag cgtgacccgg actccgctct gaacgccgcc
    22921 tcctcgcctg accggcaccc ggtcgcaacc catcaccgtc cggtcacggt gtaccggccc
    22981 gaggtcggca agatcctcgc ctgggatcga aacatcggcg agttcagctg cagcggcagt
    23041 atcatcgaca cccgcagcct gcggctcgtc ctcactgcgg gccattgcgt ctatgccgac
    23101 ggggtctggg cgcggaggca catcttcatc ccgaacttca agaacggccg ccgtccgttc
    23161 ggggcctacc gggtcaaggc cacctgggtg tccaatgcct ggttcaacaa ttccttcggg
    23221 acccggggca tgaatttcga catcggactg ctggtcaccc gcaaacggtg gaacggctcg
    23281 cggatcggag agaacgtcgg ggcgatcccg atccaaacct ttccccgccg atacggggtc
    23341 accgacatct acgggtaccc gggcggggcg atgcggaccc gggtgatgcg tacttgccgt
    23401 tcccacacca aggcggactg gtacggaggc cggttcttct acggaccgac cggccttctg
    23461 gcccgctgca acatggcggc cggctccagc ggcggcccct gggtttcccg ttacaaggcc
    23521 gaaggaggcg gcacgatagg agtggtcgac ggtctgacca gcaccggctt ctcccagggc
    23581 ggcaggtcct acttgaccag cccctacttc ggccggatcc tggtccgcct gatcaaagcc
    23641 accgaaggcc gctgaccccc ggggggcgaa tccccggccc ccggcactta cctcctcaat
    23701 ggagcgatcc gttgagaaag ggtcaggcga tgtgtatcgt gatgtgtatg aggaccaaca
    23761 tcgtcatcga cgacgaactg atggccgaag cccagaagct gaccgggaca aagaccaaga
    23821 aggaaacagt cgatctcgcc ctgcgggaaa tggtggccca ccgccgccgg caagggatcc
    23881 tggagttgcc gggcaagttc aagtgggaag gcgacctgaa cgagatgcgt cggaatttct
    23941 tccggaagtt cgattgattg tcgctgacac gagcgtctgg gtcgactatc tgaacggtgt
    24001 ctgggcaccc ggttcgagag agctccgtcg gctcctcgag gaggacgaaa ccgtgtgcat
    24061 gaccgatcac gttctctctg agattcttca gggctaccgc agggaggcgg aagcggatga
    24121 aatcgcaagc ctcttgctca ccttcccctt tctgggcctg gagggtccag gcgattacct
    24181 ggccgcagcg agcgtctatc gaaaggcccg gtctgcggga ataaccctcc gcagcacggt
    24241 cgatctcctg atcgccattc cttgcattcg tcgtgaagcc ttcctcctgc acaacgatcg
    24301 cgacttcgac caacttgcca gggtctcgga cctgaagatc tggcagccgg ccggctgacc
    24361 cgggccggca ccgggatagg tagcccgttg agctttgccg tcagcaggag ctggcgggct
    24421 tcaccgtgag gctgaccttt cgggcgccgg gcctggtctg cagtctcaac ttcggggagc
    24481 aggcagcgga ggtgaccctg gcccccttga cgatggcctt gtacccggtc gggtaggtgt
    24541 agcggggtac cgagacaacg ctgggaagcg gggcggggaa tttgccccct ccacgctttg
    24601 ccgtgccgta ggtcaggtcc atcacccggg tgtccggatc gaacttcagt gaggtgggcg
    24661 tgccggtgac ggccactgga taggggcggg ccagcgcctg gattacgtcc tgggtggcgg
    24721 tgccggtgcc tgcggttacc gatccgtcgg gtaccacgtt ctcgttgtag gcccagaaga
    24781 tccaggagac gatccgggca tcgtagatgc tggtcagccg gtcgatcgtc gcgttatcgc
    24841 tggtcgcgcc gaactcggtc aggatcgccg gaacctggtc ccgggtggcc gccttgaccg
    24901 tctccttaac catcagggcg tcgttttcgt cggtcgcata gttgtgggcc gatagccagg
    24961 cgccggttga ccccggcaaa acggtcggac ggccgaggtt gaagaagagg aacggctcga
    25021 caaacaccgg ctgggtcttc gtcaacttgc gggtcgcctt gactgccttg cgggcgaagg
    25081 gcatcatcag ttcgcgctcg agttgcgggc agccggttgc ttcggcgcag ggcgcgaagt
    25141 cggtcccggg ccacggctcg ttgaagacgt cgtaaccgac gacatggggg ttgccccgga
    25201 acttcttgac cagggcggta aggctctgga tcacgtattc ctgcaggccc ttcccgttga
    25261 cctccgagtt gtcccagaag tgatcccagg cccgctgcat ggcggtgttg ccgaagtagt
    25321 agacggggaa tggcagatcc gggttcggca ggccgtcgtc gattgccatc cagtcgggaa
    25381 agccgttgcc gttgtatttg ggggcgtaac cgtcctggtg gatgtcgacc tgggtgtaga
    25441 tcccggcctt ggtcaactgg ttgaccgtct gcgccagctt gtcgatgtag gcggtctcga
    25501 tcttgccggg ggtcggcatg aggccacgca ggtcgacccc cagccgaacc acgttgagtc
    25561 cgagcttgct cagcaggttg acgtcatcgg ctccgaatcc gtctgccgcc gggtaatacg
    25621 gcgccgtctt gtagacctcg ttgaccccgc ggagctgcac gacccgcccg gtcgcatcca
    25681 cgaaccagcg gcctttgtga gcgatcgggg caaccgcgcc ggaacctgcc ccggcgggaa
    25741 ccttggcggc ctgtgcgaac ggcaccgcga cgagcgacgc cgctacggcc agggtggcta
    25801 gaccagcgcg aatcgggttc gtgagagtgc aaacagtagt cgaacccgga agtagctcag
    25861 tctttttggc aagggtcttt gtcagaaatc cggtttcccg gcgactctcc atgtgccagc
    25921 cagcaaacga gggggtgccg atgtggatag tgactgaccg tggtttcttt tcagtggtgg
    25981 acaagggcga gcccgagggc tgcctgtgtg tacgggcccg ggtccgtgag gaccttgaga
    26041 atctctgcca gctcgagtcc ctcacctcgt acgccgacac gatcgaggaa agcaggttca
    26101 gtgactaccg cttccggatc cacgtgaagc gggaggactg ggtcaaggcc gcagccgtcc
    26161 tggccgaaca gatcgactac gacaacttca agaacgcggt caaagctcgc cagggaagag
    26221 aacgggccgg cgtgtacacc caggtctgga acgaccttta cggaatgcag gagccgttcg
    26281 ccaggtaaga ccagcagcag acatctatca agagcggtcg agggaaccgg ccccgtgacg
    26341 ccgcagcaac cagtcgggat tcaacccccg gaaacggtgc taaagccgga atcgatggag
    26401 gtaagaatgc cgtaccaatc agagatcgac gccgagttca gcgcaattga tgcgattctg
    26461 gacgagtaca cgaagcgagt tcccgagggc ccgcagggac gaatatgtca gtcatgcggc
    26521 gaggcagcga gattgccttc gatcgaacac gagatcgccg cttacgagca gcggggcgac
    26581 ttcatcgcct tctgggatga gcaccagtcg tgcttcacgc gagcctgcag agggatcttc
    26641 actggctgga gattttgcga agtctgtcgc tgcgaaaccg cggctacacc tgaaaaccgc
    26701 gctgggctta gctggttgtg ctctgactgc cgtgagggct tgcggctcgg gtatcgaggt
    26761 gaggggcagg tcctggagaa actcgcccga tggcgaggtg cgcaacacgg tacgttttgc
    26821 ggtgcctgga ggagtctgcc tctcaatgtc cccacggaga atgacgtcgc tgtcgcaatc
    26881 gagccgcccg gaggatggat tccgatcgga cttcgtgaat gtcccacctg taattgggga
    26941 accggattca cttattcggc tggctacgac ggaaaccccg acagaagatc tgcccgttgt
    27001 cgatgtgaaa tcgcccgatg cccctgcggt gcccgctcca ccaccggtga agctcagacc
    27061 tgctgttact cggtgagcgg tggcggctgg cgctactcct ccggcatcgt ctcccagttc
    27121 ggccgctact gccgggattg tctccacgag aaaggggtca aaaccacgag ctactcggcg
    27181 gcaatcgaga agcttccatc cgacaaacag gtcgaggtcg agaagcttcg caataagtgg
    27241 acggtcgagg atgcccggag gaagccggaa tagtcaggcg ggcggctgtg tcttgaggag
    27301 ccggtcattc tcgaggcgtg ccgaacggac tgctgccgga acggaaggac tcgttcggtt
    27361 cgggaacgtc gattggcgtg tacccactca ccttggacat cgaactctca cgaggccggc
    27421 ccaccgggtt gttcggtgtg gacccggacc aggaggtcgt cgatccggtc gaggtcgatt
    27481 ggcgcgattt cttcctcttc gaagaagacc tgatcgtcga agaggccgta gagggtgccg
    27541 agcacttcgc cgccgtattt cagcttcagg ttgatccggg cgccccggcc gaggcggggg
    27601 attgtcgcgg cgatctcgtc tctcgagggc atgggtggag cctag
//
LOCUS       DAOQKN010000009        27534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_701_length_27534_cov_5.977801, whole genome shotgun sequence.
ACCESSION   DAOQKN010000009 DAOQKN010000000
VERSION     DAOQKN010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 27534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27534
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_701_length_27534_cov_5.977801"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            499..1053
                     /gene="pyrR"
                     /locus_tag="PLJ64_01300"
     CDS             499..1053
                     /gene="pyrR"
                     /locus_tag="PLJ64_01300"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HNC92159.1"
                     /translation="MNEKVVLEANDVERTLRRIAHEIVEKNPDVSDLAIVGIHTGGAY
                     LAERLNSMIAGLIGEAPPLGEIDISFYRDDIGIRGSDYHPVVHSTRLDFPLQDRTVVL
                     VDDVLFTGRTVRAAIEALFDFGRPARIQLAVLCDRGHRELPIRPDYVGKNLPTSRSEQ
                     VSVRLKETDGEDGVAILSPVEAVE"
     gene            1050..1997
                     /locus_tag="PLJ64_01305"
     CDS             1050..1997
                     /locus_tag="PLJ64_01305"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HNC92160.1"
                     /translation="MKHLLSIDDLDQEQIEALLDRAESFAEVSRRDIKKVPALRGRTI
                     VTLFYEASTRTSSSFELAAKRLSADVVSVKASGSSVEKGESLKDTVETLSAYDPAAIV
                     IRHPQAGAAQMLSHWTDAAIINAGDGMHQHPSQALLDLFALRQRIGSVEGRKVWIVGD
                     VLHSRVARSNIQAFSMMGADVTIAGPPTLIPRGIEASGCEVRHSLDGIEEADVVYLLR
                     MQRERMEESFVPSIREYAQLFQVNSRRLNPRQLVMHPGPVNRGVEIGGDVIDSDRCLI
                     ADQVASGLAVRMAILFELLAHGGDARETADDPVPIGESA"
     gene            1994..3388
                     /locus_tag="PLJ64_01310"
     CDS             1994..3388
                     /locus_tag="PLJ64_01310"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNC92161.1"
                     /translation="MNEVMDGLPDEGPETGALSAPLVQRPGPDATLLIRGASLLDPVA
                     GIDGRYDLLIEDGRIARLGDAGGVEAGDGVEVIEAEGLHVFPAFFDPHVHFRTPGQEY
                     KEDLETGTRSAAAGGYGGVIAMANTSPPVSSAEQIEALRDRASRVASIPVGFSATVTR
                     DMKGTELTDMAELRQAGAVAFTDDGLPIASPRILRRALQYQAMTGGRIALHEEDPELS
                     GKGVMNEGVVSSELGLRGVPSISESTMIGRDAAIAGYEGGAIQIQHLSAVESIEQIRR
                     AKADGVDITCEVTPHHLCLTDEAVRDLDASKHKMNPPLRTETDRQALIEALVDGTIDC
                     IATDHAPHAIHEKEVPYEAANMGVTGLETAFSSIYTELVLPGRISLELLVEKLGCGGA
                     PFDIEPFSLAEGSRANLCLVDLEAEWVVGEDGYESRSVNSWCAGEKLRGRVLVTLADG
                     QVAFRLRSFSLGVA"
     gene            3385..4512
                     /gene="carA"
                     /locus_tag="PLJ64_01315"
     CDS             3385..4512
                     /gene="carA"
                     /locus_tag="PLJ64_01315"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HNC92162.1"
                     /translation="MSEATAGYVLLEDGSRFDGILCGATEGGVGEVVFNTAMTGYQEA
                     VTDPSYAGQIITFTYTMIGNYGVSAGAMESGAAHARGVIMRDARNNSDAASAEGGWLD
                     WLEANGVWAITGVNTRALVRHIRDKGAMRGGIFPASLSTGDAMARIEAEPTMAGSDFA
                     SQVSPEAPIEHDGSGPHVVALDTGIKASIVNQFLERDCRVTLLPCLTPAEEVMAREPD
                     LLFLANGPGDPAAVSAVTETVRELIGTRPMVGICLGHQLLSRAVGLETFKLPFGHRGS
                     NHPVKDLETGVIEITSQNHGFAVVGPDGKGTIENDEPVRWQTGHGEAVLSHINLYDRT
                     VEGLRLPDAKAMTVQHHPEAGPGPRDARHLFDRFLDLAKEG"
     gene            4519..7596
                     /gene="carB"
                     /locus_tag="PLJ64_01320"
     CDS             4519..7596
                     /gene="carB"
                     /locus_tag="PLJ64_01320"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HNC92163.1"
                     /translation="MPKREDIKRIMVVGSGPVVIGQAAEFDYSGVQACKVLLEEGYEV
                     VLVNSNPATIMTDPEFATRTYVEPLLPGPVAAIIEKERPDALLPTLGGQTALNLAMDL
                     SADGTLEKFGVELIGANRDAIIRGEDREVFRQVMTEAGIRIPWSITVESVEQALKELD
                     EGDASLPAIIRPAFTMGGQGGGIAETRDELIEVVSKGIAASPIGQVLVEESVIGWGEF
                     ELEVMRDRNDNVVIICSIENVDPMGVHTGDSITVAPAQSLTDPVYQSLRDIAIAVIRE
                     VGVETGGSNVQFAVNPADGEIVVIEMNPRVSRSSALASKATGFPIAKMAAKLAVGYAL
                     EEIPNDITRATPASFEPTIDYVVTKIPRFAFEKFPGADEKLSTYMQSVGEVMSIGRTF
                     RESFAKALASRELDSRPEVPESDNGLLNAIATPTPGRIDLVLAAFERGLTVEQVHEQA
                     KIDPWFLRELELLATDGDGTGGLERIYNSVDTCAAEFEAATPYYYSSHEPEGSPSEIR
                     RGEKESVVILGAGPNRIGQGIEFDYCCVHAAMTARECGRDAVMVNCNPETVSTDYDTS
                     DRLYFEPLTLEHVLAVCEKEQPEGVIVMFGGQTPLKLAEGLKEAGINLLGTPVEAIDA
                     AEDRGKFGALIDRLGIKPPPYGTALSAAEAEEIAAGIGYPLLVRPSFVLGGRAMEICY
                     STGDLKAYLDRHVKASPEHPLLLDRFLENAIEVDVDALCDGEEVFVAGIMQHVEEAGV
                     HSGDSACVLPPMSIGQEMIDEIKQATTAIALELGVIGLINIQYALAGGELYVIEANPR
                     ASRTVPFVSKAIGIPLAKVATRIMLGQKLADQDLPEFTGDHVSVKEAVLPFSRFQGAD
                     SVLGPEMKSTGEVIGIGPDFPTAFGKAQAAAGVSLPESGAVFISVTDTDKAAATQLAA
                     QLHDLGFEILATTGTAQAISRMGTPVRAINKIAEGSPHVVDMIEEQHCDLVINTPTGS
                     GARADGYEIRSAAIRHGIPSVTTLTGASAAVRAMAARANGSGEVKSLQELHAR"
     gene            7586..8464
                     /locus_tag="PLJ64_01325"
     CDS             7586..8464
                     /locus_tag="PLJ64_01325"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HNC92164.1"
                     /translation="MPADPAATPDRVQAPFGRRLCEVAETRPSGGYRIFTLIDPEGPA
                     PAPGQFFMLAAAPDWAREGGRPYLPRAISHARFTRHPGSGQRLDFLIDEVGPGTSRLA
                     EMKPGDQCWVAGPFGGRFSLPRKLNPQAAGAILVGGGIGIAPMAVLRRELVDRGIPSR
                     SLLGFRDQAHSGGVEELFGCEETRLAFEDGSSGHKGYVTDLLLQLLEGDDSASAAVYS
                     CGPPAMLEAVRRICLEREVPVELALESPMACGYGACFGCAVPKADGGYLRLCIDGPVV
                     RGDEIATAMAGEPAVD"
     gene            8496..9413
                     /locus_tag="PLJ64_01330"
     CDS             8496..9413
                     /locus_tag="PLJ64_01330"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HNC92165.1"
                     /translation="MDLSTGICGIPLSGPVLNGSGTFDALAARRVFGDALIEDFPFDC
                     YVSKTITMNPRPGNPPYRLWETPSGMINSIGLPGPGLDGFLENDLGALAEIGVPLIVS
                     VMADSAEDFATLVERVAARDEVAGIELNVSCPNVHSGLIVGEQPAETEALMKALRPLT
                     GKPLIVKLTPNVANPQEVAVAAEAGGADSVSMINTIKAAAIDPASSRTWLGAGAGGLS
                     GPAVRPIALAQVRAVSSAVGIPVVGMGGISDAESALEFILAGAEVIAVGTENFRNPRA
                     GAAVTEGLPLALRRRGFGSLAEARAATIG"
     gene            9580..9921
                     /gene="mihF"
                     /locus_tag="PLJ64_01335"
     CDS             9580..9921
                     /gene="mihF"
                     /locus_tag="PLJ64_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HNC92166.1"
                     /translation="MAPTSKTVSSPPEAPERSLDQRMDALKRANNIRTARAQLKRDLK
                     AGRTTITEVLDEPPEYLLTAKVFDMLLAVPKYGRVKTNRILKQCKVSPAKTIGGLSAR
                     QRQELVSLLQR"
     gene            10484..11065
                     /gene="gmk"
                     /locus_tag="PLJ64_01340"
     CDS             10484..11065
                     /gene="gmk"
                     /locus_tag="PLJ64_01340"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNC92167.1"
                     /translation="MSGPSPSKVFVITGPSGVGKGSLISLLRHRIPELELSTSATTRS
                     PRTGEQQGVDYHFLDQDEFLEKVAAGEFLEHAAYSGNHYGTLRSEVEGRVAAGHPVVL
                     EIEVQGARQVAESMPEATQIFIAPPELAALKQRLEGRGTDSPEAIGRRLEVAEEELAA
                     RDEFHHVVVNDELEAALDELEAIVRGKLSQSDQ"
     gene            11062..11295
                     /gene="rpoZ"
                     /locus_tag="PLJ64_01345"
     CDS             11062..11295
                     /gene="rpoZ"
                     /locus_tag="PLJ64_01345"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HNC92168.1"
                     /translation="MIKPRVDHLLQHSDSHYAAVVLAARRARQINSYYQSLNEGGYGD
                     YPPPMVPAEPGKGALTLALEEVATGKLKYEYRS"
     gene            11336..12571
                     /gene="coaBC"
                     /locus_tag="PLJ64_01350"
     CDS             11336..12571
                     /gene="coaBC"
                     /locus_tag="PLJ64_01350"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HNC92169.1"
                     /translation="MAKILLGVSGGIAAYKAVEFVRLATGAGHSVRVLMTSAARKFVG
                     PDTFEGILGAPVLTSEFERDPMRGTFPGEELPEHDPIGHLAVAESADVYLVAPASANT
                     IAKLATGQADSILTTAFLAFEGPRLVAPAMNERMYLSGATQGNLSRLRQMGVEVIDPG
                     VGRLASKGEAGVGRLPEPTELLERVEAALDEEKSADLEGLRVLVTAGGTREGIDPVRY
                     IGNRSSGRMGLALAERARKRGAEVTVICANVNLPAPAGVRRVDVESTVDLARACREQF
                     PAHDLLLMAAAPADFTVADTSNEKLSRTGGEFTLTLVPTEDILAGLAANRIDGQTVVG
                     FAAQHGGDAIGRARQKLERKGADMIVLNDVSDRTIGFDSDENAVTLVTGSGEREIPKA
                     PKTEIADRILDRAVELRAG"
     gene            12571..12987
                     /gene="dtd"
                     /locus_tag="PLJ64_01355"
     CDS             12571..12987
                     /gene="dtd"
                     /locus_tag="PLJ64_01355"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNC92170.1"
                     /translation="MRALVQRVTRASVAVEGEIVSEIGPGMLVLLGVKKGDTEAEAEK
                     VAAKLLKLRVFEDGEGRMNEPPGEREILCISQFTLYGDTRKGNRPSWIEAAGGEEAEP
                     LYDLVCEKLDAKKGIFGADMKVELVNDGPVTLLVET"
     gene            13169..13606
                     /gene="rimP"
                     /locus_tag="PLJ64_01360"
     CDS             13169..13606
                     /gene="rimP"
                     /locus_tag="PLJ64_01360"
                     /inference="COORDINATES: protein motif:HMM:NF036577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HNC92171.1"
                     /translation="MNPTPEDIEKRLEAIDPEIEFLALESAGGDALRLFIDRPGGVTL
                     DICQQVTSGLQDVLVDHSLEVSSPGPERPLTKPGHFDRFSGRRAKVVTVEPLDGRRNF
                     TGTIASAGESGVVIESDGQNFDIPYASIERSHLVPEIPQGAAK"
     gene            13603..15309
                     /gene="nusA"
                     /locus_tag="PLJ64_01365"
     CDS             13603..15309
                     /gene="nusA"
                     /locus_tag="PLJ64_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNC92172.1"
                     /translation="MTQEIVDAVRALEQEKGIAADTLMDALADALLSAYRKSPGSAKY
                     AEVDLDHETGDFRVFELILPAELEEKLLEEAAAEQERTINPETGEYNEPEEPELDPEM
                     LDPYRDQIERNDVTPDDFGRIAAQTAKQVIYQRIREAERQMMYEEYRDRVGELITGLI
                     QQSDKRYTLVQLRERVEALLPKSEQVYSERYDHGMRVKAVITDVSESTKGPSIVVSRR
                     SPELIKGLFELEVPEIADKLVEIVGVAREPGYRSKIAVVSHADGVDPVGACVGPRGSR
                     VRMVVSELRGEKIDIIPYNEEPARFVAKALSPARVREVLVDDEDMQATVVVPDDQLSL
                     AIGRDGQNARLAARLTGWRVDIKSESEFAREESEHDYEGEEEFDGRCMAVLSSGRRCP
                     NAAVSGSTYCQLPQHQALSRFSTSHVGVLASLSDEDVSVLADPDADEAAVNAIVTAAE
                     ADFVEPTDEDAGEEQVAEIEEAEAETTANEMLVEEAEADLESDDEAAAEVVAEEEAGE
                     ELAAAEEAEAEAELEEAEEAEAEEAEEAEASPEPVDEAEEADQEVAAADEAAEATEGE
                     AE"
     gene            15306..17708
                     /gene="infB"
                     /locus_tag="PLJ64_01370"
     CDS             15306..17708
                     /gene="infB"
                     /locus_tag="PLJ64_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNC92173.1"
                     /translation="MSKRRVHEIAKEAGVASKDLLAALQAAGIEVKAAASNVEEADAK
                     KVLASLGGKSGEKKPAAKSKAPAKPKAEAKPKEAAKPKAEAKPAAASKAEAKPAAEKP
                     AAKAGSTPKAEAKPKPAARPSQASSKPVRTDAAPSGTPAAPPAASGSPASSGAGGGSA
                     AAGKKRRRVVIDSQASRREQGGAPPPQRPPRRRGGRRRRPLLEEPEPVQADPSEPQAT
                     KVNSGVTVRDLAELLGLSSADLIKKLMTMGEMATLTQTLTDDSVKAIADEYDRKIEIV
                     SAAQEEAPAPEFEDSDEELMDRPPVVTIMGHVDHGKTSLLDAIRETEVAAGEAGGITQ
                     HIGAYQVHHDDHTITFLDTPGHEAFTAMRARGAQVTDVAVIVVAADDGVMPQTREAID
                     HARAADVPILIAVNKIDKEGANPDRIRTDLAADGLSPEDWGGDTIYCNVSAKTNEGLD
                     NLLDMIILVTELEELVANPDAPASGSVIESKLDPGRGPVVSVLVNRGTLRVGDSVVAG
                     AVWGKVRAMHDYLGAKVDEATPGMPVEVLGFDGVCEAGEFVQVVENDRRARQMAQERL
                     NRLKAETIARRSARKVTLEEIFARAQTGDLKELNIVLKSDVAGSLEALQDEIAKVPQE
                     QVAINIIRAQTGGITESDVMLASASNAVIIGFNVRPLADARRAAQAEGVDIRTYSVIY
                     KITEDLRAAMEGMLDTVEVEDSIGEAEVREVFKASKVGRIAGCVVTEGRVQRDASVRL
                     VREGTVIWTGQLDSLRRFKDAVQTVEEGQDCGIVLDGYADVKVGDVLEFFSTRQVEQT
                     LS"
     gene            17734..18081
                     /gene="rbfA"
                     /locus_tag="PLJ64_01375"
     CDS             17734..18081
                     /gene="rbfA"
                     /locus_tag="PLJ64_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNC92174.1"
                     /translation="MRRVNEVLRQVISDAITNDLSDPRLEMATVTSVDTTPDLRHAKV
                     YFTVLGDDAAKADALKGLGSAHSLLQGKVAAETRMKHTPALAFEYDESIESGMRIDAL
                     LAEGEAKGREDGE"
     gene            18078..19094
                     /locus_tag="PLJ64_01380"
     CDS             18078..19094
                     /locus_tag="PLJ64_01380"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HNC92175.1"
                     /translation="MNPAAASQLVVKGDLEEIATLIREEQKFLLTTHEGPDGDALGSL
                     LGMHHLLESLGKDSVMFLAAKEFPLPIEYRHLPLEGVFHEPPADIRDRIVVFLDCGNI
                     DRMPVDFLREDGKLMVNIDHHHDNTEFGDLNLVDAGASCTAEIIYELSVLLDVKVSPA
                     MARALYIGIVTDTGKFMYDATRPKTHRVAAELIEAGVDVDDVNRRLYESMPIEKLRLL
                     SRALDNLELHCEGQLIVSYITDEDYRKSGAGEEMTEGIIDNLRAVDGIRVAAVVRDQV
                     SRGRAARKISLRAGDDHTDVSVLARKFGGGGHVRAAGCSTELSFDEIVSELCSGLGDQ
                     PLPS"
     gene            19114..19962
                     /gene="truB"
                     /locus_tag="PLJ64_01385"
     CDS             19114..19962
                     /gene="truB"
                     /locus_tag="PLJ64_01385"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNC92176.1"
                     /translation="MATDPEDGVLIVDKPAGLTSHDVVAGVRRERGGKVGHAGTLDPF
                     ATGVLTILLGRATRLQRYLLELPKTYVATAKLGWKSTTGDPDGVLTETGNLPDRLELP
                     TGRIQQMVPMTSAVKVEGERLYKRAHRGESSEERPVREVEIYRAELLDAQTGPPFVGQ
                     EVSFEVEVSSGTYVRTLVETLGDAYCSKLRRTAVGPLDISLAGRVLAPLEALSFLPQI
                     ELDAEQARAIGFGQKLDTGRFDLPSGIQAGSSKSFALVHGDNLIAVARIEDSVLRPEV
                     VLVPAS"
     gene            19959..20843
                     /locus_tag="PLJ64_01390"
     CDS             19959..20843
                     /locus_tag="PLJ64_01390"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HNC92177.1"
                     /translation="MIKVTPLDEAEYRPRKIAIGTFDGVHVGHRAVIKGADTVLTFEP
                     HPLEILHPAAAPKLLMPLDVRRDVLDGLGVEEMIVIQFDDSFTKITAEQFIEEILIAR
                     LGAEEVSVGDNFRFGQKAKGTPEMLAARPEFRTRVEPLVEVDGEAVSSTRIRALVAAG
                     DMELARRCLGVPFMVEGEVVTGDQRGRELGFPTANIVPDDRFAHPGHGVYAAFADGLP
                     AAVNIGVRPTFDTGRGVLIETYIIDEEVDLYGRILRVAFVERLRGEKRFDSVDDLIAQ
                     MKRDVEKTKAVCASFHRP"
     gene            20885..21154
                     /gene="rpsO"
                     /locus_tag="PLJ64_01395"
     CDS             20885..21154
                     /gene="rpsO"
                     /locus_tag="PLJ64_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNC92178.1"
                     /translation="MTLTKENKAELISKYGKSEGDTGSTEVQVALLTERINDLTGHLR
                     EHGKDHHSRRGLLMMVGKRRRLLRYLERTDVERYRALVADLGLRH"
     gene            21151..21603
                     /locus_tag="PLJ64_01400"
     CDS             21151..21603
                     /locus_tag="PLJ64_01400"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNC92179.1"
                     /translation="MIEVGAKAPDFTLPDHAGQEVSLSDFSGRRVLLAFYPLDFSPVC
                     SDQLAQYKAIQGELEEAGVTLLGVSVDSAFAHKAFRDQLGIETSLLADFEPKGAVAEA
                     YGAYLGQAGHSNRSLVLIDADGVVEWVHETATPLDIPGPDLIVEAIRA"
     gene            21609..22058
                     /locus_tag="PLJ64_01405"
     CDS             21609..22058
                     /locus_tag="PLJ64_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92180.1"
                     /translation="MKPWIASALVLAALAAGCGGGSDDSPSPAPPAGEIKVIAESDAD
                     QKIANDLAKYLDRNCPASVRQSDNPTLQQLYTATSEFCGGVTAIEVRDKRITVQSDLE
                     PGAGSETLGNVFCVYVAGSDVADFTPGHTLLDASGKKIKTCEARPLP"
     gene            complement(22079..22771)
                     /locus_tag="PLJ64_01410"
     CDS             complement(22079..22771)
                     /locus_tag="PLJ64_01410"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNC92181.1"
                     /translation="MSPEHRQIRESLGAWAVGHLDPDEKAAVDAHLRSCSECREEAES
                     LRQAGALISLVDPAIADSLSEPLPRPSPELGRRVMAEMRDPEPAAEKMADGRRRWWLA
                     LPVAGALAAAAVSLVLVLGGGDGEGTGNSGQQFAFGSLPAGVTITGNLEPKATGTGIE
                     VRVDGMKPGTLCRVYVKTSAGTLEPAGTFRYRYDTDEHESYPATLSTAWDLSEIDSLV
                     IRAGPETFETGL"
     gene            complement(22761..23267)
                     /locus_tag="PLJ64_01415"
     CDS             complement(22761..23267)
                     /locus_tag="PLJ64_01415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNC92182.1"
                     /translation="MPGEQVREAYRAHSGELFGFACRSLGDRDRAEEAVQECFLKAWR
                     SRASYDPERGSERTWLFAILRNVIADLGRSDGRRPAGAPLEESADRIPSTDQPFERAM
                     LAWQVEEALRRIGERHRQIVVETCLRGRPYAEVAAALGIPEGTIKSRVYYGLRAMRDA
                     MEELGYES"
     gene            complement(23320..23910)
                     /locus_tag="PLJ64_01420"
     CDS             complement(23320..23910)
                     /locus_tag="PLJ64_01420"
                     /inference="COORDINATES: protein motif:HMM:NF015596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92183.1"
                     /translation="MDRRGLATLAVVLAGTLILAGCGGGDSDSGGSYGSSDNGSSAGT
                     GYGNEAGSQSADSTGKSDYGSNGSGAAGGSMAAGAAVVSAANVGDLGKVLVDDKGMTL
                     YMFEKDKGGKSACYGGCAEAWPPLATDGDPVAKNGAVAGKLGTTGRTDGSTQVTYAGW
                     PLYLYAGDQKPDQAKGNDIDQFGAEWYALTPSGEKP"
     gene            24207..26441
                     /locus_tag="PLJ64_01425"
     CDS             24207..26441
                     /locus_tag="PLJ64_01425"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNC92184.1"
                     /translation="MSIFETTRVETRVGDADISFETGKLAKQASGSVVVKSGDTMVLS
                     TATMGGLRDVDFLPLTVDVEERMYAAGKIPGSFFRREGRAGEKATLTARMIDRPIRPL
                     FPKGWHYETQLVTIPMSVDQEHPYDILAMNGASAALAISEIPVAKHVGAVRIGKLDGD
                     FVINPNEEDHELLDLDLIVAGTDEAILMVEAGANGVTEADILDALDIAHGEIKKITAT
                     IEELRQKIGKEKLVIEEPTIDESLLSDIRNSHGQALVDAIATTGKLERYEAIDQVKEA
                     VVEQYSAGIADEFEADARKSEVGKAFDAIEKDTIRKAIAVDKKRPDGRAQDEIRPIEC
                     EVDVSPRVHGSALFTRGETQILGNVTLGTTRMDMRVDGLGLETTKTFWHHYNFPPFSV
                     GEAGFMRGPKRRDIGHGALAERALAPSVPDQEEFPYVIRAVSETLESNGSSSMGSVCA
                     SSMAMQAAGIPVTAPVAGVAMGLIKEGDDYIVLTDIAGVEDHLGDMDFKVAGTADGIN
                     ALQMDIKITGVTFEILTDALEQARKGRLFILGKMAEAIDGPREQLSPHAPRIESIKID
                     QEKIGAVIGKGGETIRGLCEEFEAEIDVEDDGTIRIYAPTGTQVEDAVARIQSMTKEA
                     EVGDRYTGAKVVKTTTFGAFVELTKGTDGLLHISNVKPGGRVETVEEVLEQGQEIDVT
                     VAEVDRERGRIGLRMTDDPAIAGKSPEEIAALAANSGGGGGRRGGDREGGRRRRDRGD
                     RDRR"
     gene            26467..>27534
                     /locus_tag="PLJ64_01430"
     CDS             26467..>27534
                     /locus_tag="PLJ64_01430"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNC92185.1"
                     /translation="MSTDEPRLSETAAGVRVITEVVPSVRSVALGLWVRTGSRDETLA
                     QAGVSHFLEHLLFKGTPNYSAIEISERFDGLGASFNAATGKETTHLHARFLDEHTADV
                     FSLLGEMLLESTFPDIDSERDVVLEEIAMYEDEPSDRVHDYLADAIFGDTPLGRRILG
                     RSEVISAIPVPDIASYHRARYSANNIVVAAAGNVDHDEILAMAEKLAPGTGSGEETLV
                     PQVPDPAGRLEFAVKDTEQYHVCIGAPGINRADERRFGLAVLDSILGGSSSSRLFIEV
                     REKRGLAYSVGTYSEQFTDTGVVATYVGTREDNVEEACEVIGTELKRIQCEPVTAEEL
                     DRAKEHVKGRLVLSGESTAARM"
BASE COUNT         5035 a         9206 c         8984 g         4309 t
ORIGIN      
        1 cgttcacatc tcttcccgct atcacgtcgg taaggccctc gaggaggcca gggcggtctt
       61 caccaggacg gtcgctcccc gcgacttcga cacggtcgaa atccccttcc ccgaacgagg
      121 cgaaccgatc ctcgtcccaa acggagccag gcgcgaaacc cagccagcca ccgagtagga
      181 ctcccggaaa tggccacggc caaaggagcc cgatcgaacc tcaaaggtgc cgcacatcca
      241 gcaccgtaac ggcgccagaa ttgcggcacc ttcggccaac atcgactccg gtagccgtgg
      301 gggctgggag gggtctccgg gatgctcagg cagagcgcgg ctgaatgccg cgcgaagtgt
      361 ttgccgcttc ccggtgaccc ctgccggctc ccacggcgca ggcgcccggg atccgtggga
      421 agacgagcgg cgccggggtg agtgctatct tcatccccgt tcctttaacc caggtcctgt
      481 gaggccggga aggtgacttt gaacgagaag gttgtgctgg aagcaaacga cgtcgagcgc
      541 acgctccgtc gcatcgccca cgagatcgtc gagaagaatc ccgacgtctc ggatctcgcg
      601 atcgtcggca tccacaccgg cggcgcctac ctcgccgaac gcctcaactc gatgatcgcc
      661 ggcctgatcg gtgaagctcc gccgctgggc gagatcgaca tctccttcta tcgggatgac
      721 atcggcattc gcggctccga ctaccatccg gtcgttcact cgacccggct cgatttcccc
      781 cttcaggacc gcaccgtcgt tttggtcgat gacgtgctct ttaccggccg cacggtccgg
      841 gccgcgatcg aggcgctctt cgacttcggc cggcccgccc gcatccagct tgccgtgctc
      901 tgcgatcgcg gtcaccgcga gttgccgatc cggccggact atgtcggcaa gaacctgccg
      961 acctcgcgct ccgagcaggt ctcggtgcga ctcaaggaaa ccgatggcga ggacggggtc
     1021 gccatcctct cgccggtgga ggcggtcgaa tgaaacatct gctcagcatc gatgacctgg
     1081 accaggaaca gatcgaagcc ctgctcgacc gggccgaaag ctttgccgaa gtctccaggc
     1141 gggacatcaa gaaggtgccg gccctgcgcg gccgcaccat cgtcaccctc ttttacgagg
     1201 cttcgacccg gacctcatcc tccttcgagc tggcggccaa acgactctcc gccgacgtcg
     1261 tttcggtcaa ggcatcgggc tcctcggtcg agaagggcga gtcgctgaag gacacggtcg
     1321 aaaccctttc cgcctatgac ccggccgcga tcgtgatccg gcacccccag gccggcgccg
     1381 cgcagatgct gagccactgg accgacgccg cgatcatcaa cgccggcgac ggcatgcatc
     1441 agcatcccag ccaggcgctg ctcgaccttt tcgccctccg ccagcggatc ggctcggtcg
     1501 agggcaggaa ggtctggatc gtcggcgacg tgcttcacag ccgggtcgcc cgctcgaaca
     1561 tccaggcctt ctcgatgatg ggcgccgacg tgacgatcgc cggtccgccg accctgatcc
     1621 cgcggggaat cgaagcttcc ggttgcgagg tgaggcactc gctcgacggg atcgaggaag
     1681 ccgacgtggt ctatctgctg aggatgcagc gcgaacggat ggaagagagc ttcgtcccct
     1741 cgattcgcga gtacgcgcag cttttccagg tgaattccag gcgtctcaat ccgcggcagc
     1801 tggtgatgca tcccgggccg gtcaaccgcg gggtcgagat cggcggcgac gtgatcgatt
     1861 ccgatcgctg cctgatcgcc gatcaggtgg cctccggcct ggcggtgcgg atggcaatcc
     1921 tcttcgaact cctggcccac ggcggggatg cccgggaaac cgccgatgat ccggtgccga
     1981 tcggagagtc ggcatgaacg aggtgatgga cggcctgccg gacgaggggc ccgaaaccgg
     2041 ggcgctctcg gcgccgctgg tccagcgacc gggtccggac gcgaccctgc tgattcgcgg
     2101 tgccagcctg ctcgaccccg tcgcgggcat cgatggccgg tacgacctgc tgatcgaaga
     2161 cggccggatc gcccgcctcg gggacgcggg aggcgtcgaa gccggcgacg gagtcgaggt
     2221 gattgaagcc gaaggccttc acgtcttccc ggccttcttc gatccccatg tccacttccg
     2281 gaccccgggc caggaataca aggaggatct tgaaaccggc acccgctccg ccgcggcggg
     2341 cggttacggc ggagtgatcg cgatggccaa tacctcgcca cccgtttcat cagccgaaca
     2401 gatcgaagcc ctgcgggacc gcgcttcccg tgttgcctcg atcccggtcg gcttcagcgc
     2461 gaccgtcacc cgggacatga aggggaccga gctaaccgac atggccgagc tcaggcaggc
     2521 cggcgcggtc gccttcaccg atgacggact gccgattgcc agcccccgga ttctgcgtcg
     2581 ggctcttcag taccaggcaa tgaccggcgg ccggatcgcc ctccacgaag aggatccgga
     2641 actctccggc aagggcgtaa tgaacgaagg ggtcgtctcc tcggaactgg gcctgagggg
     2701 tgttccctcg atctccgaat cgaccatgat cggccgggac gccgcgatcg ccggttacga
     2761 aggcggggcg atccagatcc agcatctctc cgcggtcgaa tcgatcgaac agatccgccg
     2821 ggccaaggcc gacggcgtgg acatcacctg cgaggtcacc ccgcatcacc tctgtttgac
     2881 cgatgaggcg gtgcgtgacc tcgacgcctc gaagcacaag atgaacccgc cgctacggac
     2941 cgagaccgac cggcaggcgc tgatcgaggc gctggtcgac ggcacgatcg actgcatcgc
     3001 gaccgatcac gcaccccatg cgattcacga gaaggaggtg ccgtatgagg ccgccaacat
     3061 gggcgtgacc ggcctcgaga cggccttttc ctcgatctac acggaactgg tgctgcccgg
     3121 tcggatcagc cttgagttac tggtcgaaaa gctcggctgc ggcggtgcgc ccttcgacat
     3181 cgaaccgttc agtctggccg aggggagccg ggccaacctg tgcctcgtcg acctcgaggc
     3241 cgaatgggtg gtcggcgaag acggctacga aagccgctcg gtcaactcct ggtgcgccgg
     3301 cgagaagctg cgcggcaggg tgctcgtgac tctggccgac gggcaggtcg ccttccggct
     3361 tagatccttc agtctgggcg tggcatgagc gaagcaacag ccggatatgt actgctggaa
     3421 gacggcagcc gttttgacgg gatcctctgc ggcgcaaccg aaggcggggt gggggaagtc
     3481 gtcttcaaca cggcgatgac cggctaccag gaggcggtta ccgacccttc ctacgccggc
     3541 cagatcatca ccttcaccta cacgatgatc ggcaactacg gggtatcggc cggggcgatg
     3601 gaatcgggcg cagcccacgc tcgcggggtg atcatgcggg acgccagaaa caactcggac
     3661 gcggccagcg ccgaaggcgg ctggctcgac tggctcgagg ccaacggcgt ctgggcgatc
     3721 accggggtga acacccgggc gctggtccgc cacatccgcg acaagggcgc gatgcgcggc
     3781 ggcatattcc cggcgagcct ttccaccgga gatgcgatgg cccggatcga ggccgaaccg
     3841 accatggccg gctccgactt cgcctcgcag gtcagtcccg aagcaccgat cgaacatgac
     3901 ggcagcggcc ctcacgtggt cgccctggac accggcatca aggcttcgat cgtcaaccag
     3961 ttcctcgaaa gggactgccg ggtcacactg ctgccctgcc tcacccccgc cgaagaggtg
     4021 atggcccggg aacccgacct gctcttcctc gccaacggac cgggcgaccc ggccgcggta
     4081 tcggccgtga ccgaaacggt gcgggaactg atcggaacca ggccgatggt cggcatctgc
     4141 ctcggccacc aactgctcag ccgggcggtc ggcctcgaaa ccttcaagct gcccttcggc
     4201 caccgcggat caaaccatcc ggtcaaggac ctcgagaccg gggtgatcga gatcacttcc
     4261 cagaaccacg gtttcgcggt ggtcgggccc gatggcaagg gcacgatcga aaacgacgaa
     4321 ccggtgcgct ggcagaccgg tcacggcgag gcggtgcttt cacacatcaa cctctacgac
     4381 cgcacggtcg aggggctgcg actcccggac gcgaaggcga tgaccgtcca gcatcacccc
     4441 gaggccgggc cggggccgcg cgatgcccgc cacctcttcg accgtttcct cgacctggca
     4501 aaggaaggct gaaccgcgat gccgaagcgt gaagacatca agcggatcat ggtggtcggt
     4561 tccggaccgg tcgtgatcgg acaggctgcc gagttcgact actccggggt ccaggcatgc
     4621 aaggtgctgc ttgaagaggg ttacgaggtg gtgctggtca actcgaatcc ggccacgatc
     4681 atgaccgacc ccgagttcgc gacccgcacc tatgtcgaac cgctcctgcc cggcccggtc
     4741 gcggcgatca tcgagaagga acggccggac gcgctgctgc ccaccctcgg cggccagacc
     4801 gcgctgaacc tcgccatgga cctctctgcc gacggcaccc tggagaagtt cggggtcgag
     4861 ctgatcgggg ccaaccgcga cgcgatcatc cgcggcgagg accgcgaggt cttccgccag
     4921 gtgatgaccg aagccggaat ccggattccc tggtcgatca ccgtcgagtc ggtcgagcag
     4981 gccctgaagg aactcgacga gggtgacgcg tcgctgccgg cgatcatccg gccggccttc
     5041 accatgggcg gccagggcgg gggaatcgcc gagacccgtg acgagctgat cgaagtcgtt
     5101 tcgaagggca tcgccgccag cccgatcggc caggtgctgg tcgaggagtc ggtgatcggc
     5161 tggggcgagt tcgaactcga ggtgatgcgc gaccgcaacg acaacgtggt gatcatctgc
     5221 tcgatcgaga acgtcgaccc tatgggggtc cacaccggcg attcgatcac cgtcgctccg
     5281 gcccagtcgc tgaccgaccc ggtctaccag tcgctgcggg acatcgcgat cgccgtgatt
     5341 cgcgaggtcg gggtcgagac cggtggctcc aacgtccagt tcgcggtcaa cccggccgac
     5401 ggcgaaatcg tggtgatcga gatgaacccg cgggtgtcgc gctcgtcggc tttggcctcg
     5461 aaggcaaccg gcttcccgat cgccaagatg gcggccaagc tggcggtcgg ctacgcgctc
     5521 gaggagatcc cgaacgacat cacccgggcg accccggcct cattcgagcc gaccatcgat
     5581 tacgtggtga ccaagatccc gcgcttcgcc ttcgagaagt tccccggagc cgacgagaag
     5641 ctctcgacct acatgcagag cgtcggcgag gtcatgtcga tcgggaggac cttccgcgaa
     5701 tccttcgcca aggcgctggc ttcccgcgag ctcgattccc ggcccgaagt gccggagagc
     5761 gacaacgggc tgctgaacgc gatcgccacc ccgaccccgg gccggatcga cctggtcctc
     5821 gccgccttcg agcgcgggct gaccgtcgag caggttcacg aacaggcgaa gatcgacccc
     5881 tggttcctgc gcgaactgga actgctggcc accgacggcg acggcaccgg cggcctcgag
     5941 cggatctaca actcggtcga cacctgcgcg gccgagttcg aggcggcgac cccgtactac
     6001 tactcgtcgc atgagccgga agggtcaccc tccgagattc gccggggtga gaaggaatct
     6061 gtggtgatcc tcggggccgg cccgaaccgg atcggccagg ggatcgagtt cgactactgc
     6121 tgcgtccacg ccgcgatgac cgcccgcgag tgcggacgcg acgcggtgat ggtcaactgc
     6181 aatccggaaa cggtctcgac cgattacgac acttcggacc ggctctactt cgagccgctg
     6241 acgctcgaac acgtgctggc ggtctgcgag aaggaacagc cggaaggggt gatcgtgatg
     6301 ttcggcggcc agacgccgct gaagctggcc gagggtctca aggaagccgg catcaacctg
     6361 ctcggcaccc cggtcgaggc gatcgacgcg gccgaggacc gcggcaagtt cggtgccctg
     6421 atcgaccggc tcggaatcaa gccgccgccc tacggcacgg cgctgtcggc ggcggaggct
     6481 gaagaaatcg cggccgggat cggctacccg ctgctggtcc gtccctcctt cgtgctgggt
     6541 ggccgggcga tggagatctg ctactcgacc ggggacctga aggcctatct cgaccggcat
     6601 gtcaaggcct cgccggaaca tccgctgctg ctcgaccgct tcctcgagaa cgcgatcgag
     6661 gtcgacgtgg acgcactctg cgatggcgag gaggtcttcg tggccgggat catgcagcac
     6721 gtcgaagagg ccggagtcca ttccggcgac tcggcctgcg tgctgccgcc gatgagcatc
     6781 ggccaggaga tgatcgacga gatcaagcag gcgaccacgg cgatcgcgct cgaactggga
     6841 gtgatcggcc tgatcaacat ccagtacgcg ctggccggcg gcgagcttta cgtgatcgag
     6901 gccaatcccc gggcctcgcg gaccgtgcca ttcgtttcga aggcgatcgg gattcccctg
     6961 gccaaggtcg cgacccggat catgctcggc cagaagctgg ccgaccagga cctgcccgaa
     7021 ttcaccggtg atcatgtctc ggtcaaggag gcggtgctgc cgttttcccg cttccagggt
     7081 gccgattcgg tgctcggtcc ggagatgaag tcaaccggtg aggtgatcgg gatcggcccc
     7141 gacttcccga ccgccttcgg caaggcccag gcggcggccg gggtatccct tccggaatcc
     7201 ggggccgtgt tcatctcggt caccgacacc gacaaggctg ccgccaccca gctcgccgcc
     7261 cagcttcacg accttggttt cgagattctc gccacgaccg gcaccgccca ggcgatctcg
     7321 cgcatgggaa ccccggtcag ggcgatcaac aagatcgccg agggctcccc gcatgtggtc
     7381 gacatgatcg aggagcagca ctgcgacctg gtgatcaaca cgccgaccgg ttccggggcc
     7441 cgggccgacg gttacgagat ccgctcggcc gcgatccggc acggcattcc ttcggtgacc
     7501 acactgaccg gggcctcggc tgcggtccgg gcgatggccg cccgggccaa tggttccggg
     7561 gaggtcaagt cacttcagga gcttcatgcc cgctgacccg gccgccacgc ctgaccgggt
     7621 tcaggcgccg ttcggacggc gactctgcga agtcgctgaa acgaggccct cgggcggcta
     7681 ccggatcttc acgctgatcg accccgaagg cccggccccg gccccgggcc agttcttcat
     7741 gctggccgcc gcgcccgact gggcgaggga gggcggccgt ccgtacctgc cgcgggcgat
     7801 ctcccatgcc cggttcacac ggcaccccgg ctccgggcag cgacttgact tcctgatcga
     7861 cgaggtcggc ccgggaactt cgaggctcgc ggagatgaag ccgggagatc aatgctgggt
     7921 ggccgggccg ttcggtggcc gcttcagcct gcctcgcaag ctcaatccgc aggccgccgg
     7981 ggcgatcctg gtcggcggcg ggatcggcat cgcgccgatg gcggtgctgc gccgtgaact
     8041 cgtcgaccgc gggattccct cccgcagcct gctcggattc cgtgatcagg cgcattccgg
     8101 cggggtggaa gaactcttcg gctgcgagga gacgaggctc gccttcgagg acggcagctc
     8161 cggccacaag ggttacgtga ccgacctgct gctccagctg ctcgaaggtg acgattccgc
     8221 ctcggccgcc gtctactcct gcggaccgcc ggcgatgctg gaggccgttc gccggatctg
     8281 ccttgagcgg gaggtcccgg tcgagctcgc cctcgaatcg ccgatggcct gcgggtacgg
     8341 agcctgcttc ggctgcgccg tgccaaaggc ggacggcggc tacctgagac tctgcatcga
     8401 cggcccggtc gtccgtggcg acgagatcgc caccgcgatg gcaggagagc cggccgttga
     8461 ctgaatcggc aaaagcggca gaaccgctcc cggtcatgga tctctccacc gggatctgcg
     8521 gaattcccct cagcggtccc gtgctgaacg gttcggggac cttcgatgcg ctggccgccc
     8581 gccgggtctt cggcgacgcg ctgatcgagg acttcccctt cgactgttac gtctcgaaga
     8641 cgatcaccat gaacccgcgt cccggcaacc cgccctaccg gctctgggag accccgtcgg
     8701 ggatgatcaa ctcgatcgga ctgcccggcc cgggcctgga cggattcctc gaaaacgacc
     8761 taggggccct ggccgaaatc ggggtgcccc tgatcgtttc ggtcatggcc gacagcgccg
     8821 aagacttcgc caccctcgtc gagcgggtcg cggccaggga tgaggtggcc gggatcgaac
     8881 tcaatgtttc ctgccccaac gtccactccg gactgatcgt cggcgagcag cccgccgaga
     8941 ccgaggccct gatgaaggcg ctgcggccgc tgaccggcaa gccgctgatc gtcaagttga
     9001 cgcccaacgt ggccaaccct caagaggtgg ctgttgcggc cgaggccggg ggagccgact
     9061 cggtctcgat gatcaacacg atcaaggcgg ccgcgatcga cccggcttcc agccgaacct
     9121 ggcttggcgc cggagccggc ggactctcgg ggccggcggt caggccgatc gccctggccc
     9181 aggtgcgggc ggtttcatca gcggtcggaa tcccggtggt cggcatgggc ggcatcagtg
     9241 acgccgagtc ggcactcgag ttcatcctgg ccggcgccga ggtgattgcg gtcggtaccg
     9301 agaacttccg caacccccgg gccggggcgg ccgtgaccga gggtcttccg cttgctctcc
     9361 ggcgccgcgg gttcggcagc ctggccgagg cccgggcggc cacgatcggc tgagccggct
     9421 cttaccgaac gggcgcccgc cggacatgcc cggccggtga ccggttccgc ctcgccctca
     9481 acctcgagtc gaggttgaga tggatttgcc cgaaatcggc acttttcggg ggttttcggc
     9541 ctccaaactt ctcgccagtg gctatactcg ccgccttcca tggccccgac ctcgaagacc
     9601 gtatccagcc cgccggaagc cccggaaagg tccctcgacc agaggatgga cgcactcaag
     9661 agggccaaca atattcgcac cgccagggcc cagctgaagc gggacctcaa ggccggtcgc
     9721 accacgatca ccgaggttct cgacgagccg ccggaatacc tcctgaccgc caaggtcttc
     9781 gacatgctgc tcgccgttcc gaagtacggc agggtcaaga ccaaccggat cctcaagcag
     9841 tgcaaggtca gtcccgccaa gaccatcggc ggactcagcg cccgccagcg ccaggaactc
     9901 gtaagcctcc tccagcgtta acccgggcaa gcaccagcct caacccaggc aggcaccagc
     9961 ctcaacccgg gtaagcacca gctcaaaccc gggcaggcag caagacagaa cccgcggcaa
    10021 gcagggctct cccgagatcc ggttttccgc caaataccgc acccaactcc gatctcgccc
    10081 gttccccact catgatccgt gaagggtgcc gcaggtttgc gcccacagag gtaacaaacc
    10141 tgctgtaccg accaaagacg aggccgaata cccaaccctg gaggcggcaa gcagctgcgg
    10201 gccacctgaa ttcccccgtc acttgccctg ttccacccgg gtgggtcgga taaccggtac
    10261 ctgggccgga aaaccgaccc acgagtcttc cgaaccggac cgggcgttgg ggtgggtcgg
    10321 gtttccgggg ctggtgacgg aaatccgccc cagcggcatc gaggcacggt ccgggttgat
    10381 cgagatctgg gttgggggcg gtatttggca gaaagtggga tctcgcgaaa gacaggctgg
    10441 ggttgcgttg gcttccggag tggcgggtag gctggtcggt cctatgtctg gtccatctcc
    10501 gtcgaaggtc ttcgtgatca ccggtccgtc cggggtgggg aagggcagcc tgatttcgct
    10561 gctgcgacac cggattccgg agcttgaatt gagcacttcg gccaccaccc gatcaccacg
    10621 aaccggcgaa cagcagggag tcgattatca cttcctcgat caggacgagt tcctcgaaaa
    10681 ggtcgctgcc ggagagttcc tcgagcacgc cgcatacagc ggcaaccatt acgggacact
    10741 gcgctccgag gtcgagggca gggtggcggc cggacatccg gtggtgctcg aaatcgaggt
    10801 tcagggtgcc cgccaggtag cggaatcgat gccggaggcg acgcagatct tcatcgcgcc
    10861 cccggaactc gccgccctga agcaacggct ggaggggagg gggaccgatt caccggaggc
    10921 gatcggtcgc cggctcgagg ttgcggagga ggagctcgcg gcccgtgacg agttccatca
    10981 cgtggtggtc aacgatgaac tcgaggctgc gctcgacgag ctcgaagcca tagttcgtgg
    11041 caagctgtca caatcagatc aatgatcaag cctcgcgtcg accatcttct ccagcattcg
    11101 gactcgcatt atgcggccgt ggttctggct gcccgccggg cgcgccagat caacagttac
    11161 taccagagcc tcaacgaagg cggctacggc gattacccgc cgcccatggt tccggccgag
    11221 ccgggcaagg gtgcgttgac tctcgctctg gaagaggtcg ccaccggcaa actcaagtac
    11281 gagtaccgct cgtaggctgc gacccgggcc caagcccgct gaagaggtaa ccgatatggc
    11341 gaagatcctg cttggtgtaa gcggcgggat cgcggcctac aaggcagtcg agttcgtgcg
    11401 cctggcgacc ggagccgggc actcggtgcg ggtgttgatg acctcggccg cccgcaagtt
    11461 cgtcggcccg gacaccttcg agggaatcct cggtgcgccg gtcctcacct ccgaatttga
    11521 acgggacccg atgcggggca cgtttcccgg cgaggagttg ccggaacacg atccgatcgg
    11581 ccatctcgcg gtggccgagt cggcagatgt ctacctggtc gccccggcgt cggccaacac
    11641 gatcgccaaa ctggcgaccg gccaggccga ctcgatcctg accaccgcct tcctcgcctt
    11701 cgagggtccg cggctggtcg ccccggcgat gaacgagcgg atgtatctct cgggagccac
    11761 ccaggggaac ctttcccggc tgcggcagat gggggtggag gtaatcgatc ccggggtcgg
    11821 ccgcctcgct tcgaagggcg aagccggggt cggccgcctg cccgagccaa ccgagctgct
    11881 ggaacgggtc gaggccgccc tcgacgagga gaagtcggcg gacctcgagg gtctgcgggt
    11941 gctggtcacg gccgggggca cccgcgaggg gatcgatccg gtccgctaca tcggcaaccg
    12001 ctcctccggc cggatggggc ttgccctggc cgagcgggcc cgcaagcgcg gggcggaggt
    12061 gaccgtgatc tgcgccaacg tgaacctgcc ggccccggcg ggagtgagaa gggtggatgt
    12121 cgaatcgacg gtcgacctcg cccgggcatg tcgcgaacag ttcccggccc atgacctgct
    12181 gctgatggcg gcggcgccgg cggacttcac cgtggccgat acctcgaacg aaaagctgtc
    12241 ccggaccggg ggcgagttca ccctgaccct ggttccgacc gaggacattc tcgccggcct
    12301 cgccgcgaac cggatcgatg gtcagaccgt ggtcggtttc gccgcgcagc atggtggcga
    12361 cgcgattggc cgggcccggc agaagctgga gcgaaaaggg gcggacatga tcgtcctcaa
    12421 tgacgtatcc gaccggacga tcggcttcga ctcggacgag aacgcggtga cgctggtcac
    12481 cgggtccggc gagcgggaga tccccaaggc accgaagacc gagatcgccg accgcatcct
    12541 cgaccgtgcg gtcgaactcc gcgccggctg atgcgggcac tggttcagcg ggttacccgg
    12601 gcctcggtcg cggtcgaagg cgagatcgtc tcggagatcg gcccggggat gctcgttctg
    12661 ctgggagtga aaaagggcga caccgaagcc gaggccgaaa aggtcgcggc caaactgctc
    12721 aaactccggg tcttcgaaga cggcgaaggc cgcatgaacg aaccgcccgg cgaacgcgag
    12781 attctctgca ttagccagtt caccctctac ggcgacaccc gcaagggcaa ccgcccgagc
    12841 tggattgaag ccgcgggcgg ggaggaagcc gaaccgcttt acgacctcgt ctgcgaaaag
    12901 ctcgacgcga agaagggcat cttcggtgcc gacatgaagg tcgaactcgt gaacgacggc
    12961 ccggtcaccc ttctggtcga aacctgaccc ggagaacacc gcgagatccg agtttttcgt
    13021 cactgtgacg aaaaagtcgg agcccgctgt tcctggttgc tgcccgactt gatattcggg
    13081 ctatacttgt tggtgctttt cccctctagc tgtgtcgaaa agtgggcctc gcccgctttt
    13141 ttttggtcct gaacccggaa atacagactt gaatcccacc cccgaagaca tcgagaaaag
    13201 gctcgaagcg atcgatcccg agatcgagtt tctggcgctg gaaagcgccg gaggtgacgc
    13261 cctgcgcctt ttcatcgatc ggccgggcgg ggtgaccctg gacatctgcc agcaggtgac
    13321 ctccggactt caggacgtcc tggtcgacca ctcgctcgag gtttcctcac cgggccccga
    13381 acggccactg accaagccag gccatttcga ccgtttctcg ggtcgccggg cgaaggtggt
    13441 caccgtcgag ccgctcgacg gacggagaaa cttcaccggg accatcgcca gtgccgggga
    13501 aagcggagtc gtcatcgaat ccgacggcca gaatttcgat attccttacg cgagcattga
    13561 gcgctcgcac ctcgtgccag aaatcccaca aggagcagcc aagtgaccca agagatcgtc
    13621 gacgcagttc gagccctcga acaggaaaag ggaatcgccg ccgacaccct gatggacgcg
    13681 cttgccgacg ccctgctctc cgcctaccgc aagagcccgg gttcggccaa gtacgccgag
    13741 gttgatctcg atcacgagac cggagacttc cgggtcttcg agctgatcct gcctgccgag
    13801 ctcgaagaga agctgctcga agaagccgcc gccgagcagg agcgcacgat caatccggag
    13861 accggcgagt acaacgagcc ggaagagccc gaactcgacc cggagatgct ggatccctac
    13921 cgcgaccaga tcgaacgcaa tgacgtcacc ccggacgact tcggccggat cgccgcccag
    13981 accgccaagc aggtgatcta ccagcggatc cgcgaagccg agcgccagat gatgtacgag
    14041 gagtaccgcg accgggtcgg cgagctgatc accggtctga tccagcagtc ggacaagcgc
    14101 tacaccctgg tccagctgcg cgagcgggtc gaggcgctgc tgcccaagtc cgagcaggtc
    14161 tacagcgagc gctacgacca cggcatgcgg gtcaaggccg tgatcaccga cgtctccgag
    14221 tccaccaagg gcccgagcat cgtcgtttcg cgccgcagcc ccgagctgat caagggactc
    14281 ttcgaactgg aagtgcccga gatcgccgac aagctggtcg agatcgtcgg ggttgcccgc
    14341 gagcccggct accgctcgaa gatcgcggtg gtctcgcatg ccgacggagt cgacccggtc
    14401 ggcgcctgcg tcggcccccg cggttctcgc gtgcgcatgg tcgtttccga gctgcgcggc
    14461 gagaagatcg acatcattcc ctacaacgag gaaccggccc gcttcgtcgc caaggcgctt
    14521 tcgccggccc gggtccgcga ggtcctggtc gacgacgagg acatgcaggc gaccgtcgtc
    14581 gtgccggacg accagctttc cctggcgatc ggccgcgacg gccagaacgc caggctagcc
    14641 gcccgcctga ccggctggcg ggttgacatc aagtccgaaa gcgagttcgc ccgggaagag
    14701 agcgagcacg actacgaggg cgaagaggaa ttcgacggcc gctgcatggc cgtgctttcc
    14761 agcggccgcc gttgcccgaa cgccgccgtc tccggctcga cctactgcca gctgccgcag
    14821 catcaggcgc tctcgcgctt ctcgaccagc cacgtcggtg tgctggcgag tctcagtgac
    14881 gaggacgtca gcgtcctcgc cgaccccgat gccgacgaag ccgccgtgaa tgcgatcgtc
    14941 acggccgccg aggctgattt cgtcgaaccg accgacgagg acgccggcga agagcaggtg
    15001 gcggagatcg aggaagcaga agccgagacg accgcgaacg agatgctggt cgaagaggcc
    15061 gaggctgatc tcgaatccga tgacgaggcg gccgccgagg tcgtagccga ggaggaagcc
    15121 ggcgaggagc tggctgccgc cgaggaagcc gaagccgaag cagaactgga agaggctgaa
    15181 gaagccgaag ctgaagaagc tgaagaagcc gaagcaagtc cggagccggt agacgaggct
    15241 gaagaagccg atcaggaagt cgccgcggcc gatgaggcag ctgaagcgac ggaaggcgag
    15301 gccgagtgag caagaggcgc gtccacgaaa ttgccaagga agccggagta gccagcaagg
    15361 atctgctggc ggcacttcag gctgccggaa ttgaagtaaa ggcggctgcc tcgaacgtgg
    15421 aggaagctga cgcgaagaag gtcctggcca gcctcggcgg caagtccggc gagaagaagc
    15481 cggccgcgaa gtcgaaggct cccgccaagc cgaaggccga ggcgaagcca aaggaagccg
    15541 ccaaaccaaa agctgaagcg aaaccggccg ccgcgtcgaa ggctgaagcc aaaccggccg
    15601 cagagaagcc cgccgccaaa gccgggtcga caccgaaggc cgaggcgaag ccgaagccgg
    15661 ccgccaggcc ttcccaggct tcgagcaagc cggttcgcac cgacgctgct ccctccggaa
    15721 ctccggccgc tccgcctgcc gcatccggtt caccggcttc gtccggtgcc ggtggaggct
    15781 ccgctgccgc cggcaagaag cgccgccgcg tggtgatcga ctcccaggct tcacgccgcg
    15841 aacaaggcgg cgccccgccg ccgcagcggc cgccgcgtcg ccggggtgga cgccgtcgcc
    15901 ggccgttgct ggaagagccg gagcccgtac aggcggatcc gagcgagccg caggcgacca
    15961 aggtcaacag cggcgtcacg gtccgtgacc tggccgaact gctcggcctc agttccgccg
    16021 acctgatcaa gaagctgatg accatgggcg agatggcaac cctcacccag accctgaccg
    16081 acgattcggt caaggcgatc gccgacgagt acgaccgcaa gatcgagatc gtctcggcgg
    16141 cgcaggagga agccccggct ccggaattcg aggattccga tgaggaactc atggaccgcc
    16201 cgcccgtggt cacgatcatg ggtcacgtcg accacggcaa gacctcgctg ctcgacgcaa
    16261 ttcgcgagac cgaagtcgcc gccggcgagg ccggcgggat cacccagcac atcggtgctt
    16321 accaggtcca tcacgacgac cacacgatca ccttcctcga caccccgggc cacgaggcat
    16381 tcaccgccat gcgggcccgt ggcgcccagg tcaccgatgt tgcggtgatc gtggtcgccg
    16441 ccgatgacgg cgtcatgccc cagaccaggg aagcgatcga tcacgcccgg gccgccgacg
    16501 ttccgatcct gattgcggtc aacaagatcg acaaggaagg tgcgaatccc gaccggatcc
    16561 gcaccgatct tgccgccgac gggctcagcc cggaggattg gggcggggac acgatctact
    16621 gcaacgtgtc ggcgaagacc aacgagggac tcgacaacct gctcgacatg atcatcctgg
    16681 tcaccgagct cgaggaactc gtcgcgaacc ccgatgcgcc cgcatccggt tccgtgatcg
    16741 agtccaagct cgatcccggc cggggcccgg ttgtttccgt gctggtcaac cggggcacgc
    16801 tccgggtcgg cgactcggtc gtggccggag ccgtctgggg caaggtccgg gcgatgcacg
    16861 actacctcgg cgccaaggtt gacgaagcaa cccccggcat gccggtggag gtgctcggtt
    16921 tcgatggagt ctgcgaagcc ggggagttcg tgcaggtggt cgagaacgac cgtcgcgcca
    16981 ggcagatggc ccaggaaagg ctcaaccgcc tcaaggccga aaccatcgcc cgccgttccg
    17041 cccgcaaggt caccctcgag gagatcttcg cccgcgccca gaccggcgac ctcaaggaac
    17101 tcaacatcgt gctcaagagc gatgtcgcgg gttcgctgga ggcgctgcag gacgaaatcg
    17161 ccaaggtgcc gcaggagcag gtcgcgatca acatcatccg cgcccagacc ggcggcatca
    17221 ccgagtccga cgtgatgctc gcttcggcct cgaacgcagt gatcatcggc ttcaacgtga
    17281 ggccccttgc cgacgcccgc cgggccgcgc aggccgaggg ggtcgacatt cgcacctact
    17341 ccgtcatcta caagatcacc gaagacctgc gggccgccat ggaaggcatg ctcgacacgg
    17401 tcgaggtaga ggattcgatc ggtgaagcgg aagtccggga ggtcttcaag gcctccaagg
    17461 tcggccggat cgccggatgc gtcgtcaccg aagggagggt ccaacgggac gcctcggtgc
    17521 ggctcgtcag ggaggggacc gtcatctgga cggggcagct cgattcgctg cgccgtttca
    17581 aggatgcggt ccagaccgtc gaggaaggcc aggactgcgg catcgtcctg gacgggtacg
    17641 ccgacgtgaa ggtcggcgac gtactcgagt tcttctcgac ccgtcaggtc gaacagaccc
    17701 tcagttaggc ggcaagcggt gtcgaaggcg cggatgcgca gggtgaatga ggtgcttcgt
    17761 caggtgattt ccgatgcgat caccaacgat ctgagcgatc ctcgtctgga aatggcaacc
    17821 gtcacctcgg tcgacaccac cccggacctg cgtcacgcca aggtctattt cacggtgctg
    17881 ggtgacgatg cggcaaaggc cgacgcgctc aagggcctgg gcagcgccca cagcctgttg
    17941 cagggcaagg tggcggccga gacccggatg aagcacaccc cggcactggc cttcgaatac
    18001 gacgaatcga tcgagtcggg aatgcgaatc gacgccctgc tggccgaagg cgaagccaaa
    18061 ggcagggagg acggcgagtg aatcccgctg ccgccagcca gctcgtggtg aagggggatc
    18121 tcgaggagat cgcgaccctg atcagggagg aacagaagtt cctcttgacg acccacgagg
    18181 gaccggacgg agatgccctc ggctcgctgc tggggatgca tcacctgctg gaaagcctcg
    18241 gcaaggactc cgtgatgttc ctcgccgcga aggagttccc gcttccgatc gagtaccgcc
    18301 acctgccgct cgagggcgtc ttccacgaac cgccggccga catccgcgac cggatcgtcg
    18361 tctttctcga ttgcggcaac atcgaccgca tgccggtcga cttcctgcgc gaagacggca
    18421 agctgatggt caacatcgac catcatcacg acaacaccga attcggtgac ctgaacctgg
    18481 tcgacgccgg tgcctcctgc accgccgaga tcatctacga actttcggtc ctgctcgacg
    18541 tcaaggtcag ccccgcgatg gcccgggcgc tttacatcgg gatcgtcacc gacaccggca
    18601 agttcatgta cgacgcgacc aggcccaaga cccaccgggt cgccgccgag ctgatcgaag
    18661 ccggagtcga cgtcgatgac gtgaaccgtc gcctctacga aagcatgccg atcgagaaac
    18721 tgcgcctgct gagccgggcc ctcgacaacc tcgaacttca ctgcgaagga cagctcatcg
    18781 tcagctacat caccgacgag gattaccgca agagcggggc cggggaggag atgaccgaag
    18841 ggatcatcga caacctgcgg gcggtcgacg gaatcagagt cgctgccgtg gtgcgcgacc
    18901 aggtcagccg cggccgcgcg gcccgcaaga tcagcctccg cgccggtgac gaccacaccg
    18961 acgtctcggt gctggcccgg aagttcggcg gcggtggcca cgtgcgggcc gccggctgct
    19021 cgaccgagct ctccttcgat gagatcgtct ccgaactctg cagcggcctg ggcgaccagc
    19081 cgctcccttc ctgaccgaga gcggccggct tcagtggcca cggatcccga agacggggtg
    19141 ctgatagtcg acaagcccgc cggcctgacc tctcacgacg tggtcgccgg ggtgcggcgc
    19201 gagcgtggcg gcaaggtcgg gcatgcggga accctcgacc ctttcgcgac cggggtcctg
    19261 accatcctgc tgggccgtgc gacccgtctt cagcggtacc tgctggagct gccgaagacc
    19321 tatgtcgcga ccgcgaagct gggctggaag tcaaccaccg gcgacccgga cggagttttg
    19381 accgaaaccg gcaacctgcc ggaccggctc gagctcccga ccggtcggat tcagcagatg
    19441 gtgccgatga cctcggcggt caaggttgaa ggcgagcgac tctacaaacg ggcccaccgc
    19501 ggcgagagca gcgaggagcg gccggttcgc gaggtcgaga tctaccgggc ggagctcctc
    19561 gacgcacaga ccggaccgcc gtttgtcggt caggaagtca gcttcgaggt ggaagtgagc
    19621 tcgggaacct acgtccggac cctggttgaa accctcggtg acgcctattg ctcgaagctg
    19681 cgccggaccg ccgtgggtcc cctcgacatc tctctcgcgg gccgggtgct cgccccgctc
    19741 gaggccctgt cgtttttgcc gcagatcgag ctcgatgcgg aacaggcccg cgcgatcggc
    19801 ttcggacaga agctcgacac cggtcggttt gacctgccat cgggcattca ggcagggtcc
    19861 tcaaagagtt tcgcattggt ccacggggat aacctgattg ccgtggctcg tattgaagat
    19921 tcagtcctgc gccccgaagt cgtcctggtt cccgcctcgt gatcaaggta acccccctgg
    19981 atgaagccga gtaccggccc cggaagatcg cgatcggaac cttcgacggc gtccacgtcg
    20041 gacaccgcgc ggtaatcaag ggggccgaca ccgtgctgac cttcgagcct catcccctgg
    20101 agatccttca tccggcggcc gccccgaagc tgctgatgcc cctcgacgtc cgccgcgacg
    20161 ttctcgacgg tctcggagtc gaggagatga tcgtcatcca gttcgatgat tcgttcacga
    20221 agatcaccgc cgagcagttc atcgaagaga tcctgatcgc ccgcctcggc gccgaagagg
    20281 tgtcggtcgg agacaatttc cgcttcggcc agaaggcgaa gggcacgccg gaaatgctgg
    20341 ccgccaggcc cgagttcagg acccgggtcg agcccctcgt ggaagtcgac ggcgaagccg
    20401 tttcctcgac gcggattcgc gccctggtcg cagcggggga catggagctg gccagacgct
    20461 gcctgggagt ccccttcatg gtcgagggcg aggtcgtgac cggtgaccag cggggtcgcg
    20521 aacttggctt cccgactgcg aatatcgttc ccgatgaccg ttttgcccat cccgggcacg
    20581 gtgtttacgc agccttcgcc gacggtctgc cggcggcggt gaacatcggc gtcaggccga
    20641 cctttgacac cggtcgggga gtcctgatcg agacctacat catcgacgag gaggtcgatc
    20701 tgtatggccg gatcctccgg gtcgccttcg tcgaacggtt gcgcggcgag aaaaggttcg
    20761 actcggtcga cgatctgatc gcgcagatga aacgggacgt agagaagacg aaggccgtct
    20821 gtgctagctt ccatcggccg tgaacgggtc cgcccgtgac ccgaaaccaa ggatttttga
    20881 agaaatgaca ctgaccaaag aaaacaaggc cgaactgatc agcaagtacg gcaagtccga
    20941 gggcgacacc ggctcgaccg aagtccaggt ggccctgctt accgagcgga tcaacgatct
    21001 caccggccac ctgcgggagc acgggaagga tcaccattcc cgccgcgggc tgctgatgat
    21061 ggtcggcaag cgccgccgcc tgctgcgcta cctcgagcgg actgacgtcg agcgctaccg
    21121 cgccctcgtc gccgacctcg gcctgcgcca ttgatcgaag tcggggccaa ggctccggat
    21181 ttcaccctgc ctgaccacgc cggtcaggaa gtttccctgt cggacttttc cggccgccgg
    21241 gtgctgctcg ccttctaccc gctggacttc agcccggtct gctccgatca gctggcccag
    21301 tacaaggcga ttcagggcga actcgaagag gccggcgtca ccctgctcgg agtcagcgtc
    21361 gattcggcct tcgctcacaa ggcgttccgg gaccagctcg ggatcgaaac ctcgctgctc
    21421 gctgatttcg agcccaaggg cgcggtcgcc gaagcctacg gcgcctacct cggccaggcc
    21481 ggccattcga accgttccct ggtcctgatt gacgccgacg gcgttgtcga gtgggtccac
    21541 gagacagcga cgccgctcga catcccgggt cccgacctga ttgtcgaagc catccgggcc
    21601 tagccgccat gaagccctgg atcgcttccg cgctggtgct tgcggcgctc gccgccggat
    21661 gcgggggagg aagcgacgat tcaccatccc cggccccgcc ggccggggaa atcaaggtca
    21721 tcgccgaatc ggacgccgat cagaagatcg ccaatgacct cgccaaatac ctagaccgga
    21781 actgcccggc cagcgttcgc caaagcgaca atcccacgct gcagcagctc tacacggcta
    21841 cttccgagtt ctgtggcggc gtcaccgcga tcgaggtcag ggacaagagg atcacggttc
    21901 aaagcgacct cgagccgggc gccggctcgg agacgctcgg caacgtcttc tgtgtatacg
    21961 tcgcaggttc cgatgtcgcc gatttcacac cgggccatac cctgctcgac gcttccggca
    22021 aaaagatcaa gacctgtgag gcccgcccgc tgccctgacc ggccggtgcc cgcgcggcct
    22081 agagcccggt ttcgaaggtc tccggtccgg ccctgatcac gaggctgtcg atttcactca
    22141 gatcccaggc cgttgagagg gtggccgggt aggactcgtg ttcatccgtg tcgtagcggt
    22201 aacggaaggt tccggctggt tccagcgtgc cggccgaggt cttcacgtaa acccggcaca
    22261 gcgtgccggg cttcatcccg tcgaccctga cttcgatccc ggtcccggtt gccttcggtt
    22321 cgagattgcc ggtaatcgtc acgccggccg ggagggagcc gaaagcgaac tgctgaccgc
    22381 tattcccggt tccctcgcca tcgccgccgc ccagcaccag aaccaggctg accgcggccg
    22441 ccgccagcgc cccggcgacc ggcagggcga gccaccagcg ccggcggccg tccgccatct
    22501 tctccgcggc tggctccggg tcccgcattt ccgccatcac ccggcgaccg agctcgggcg
    22561 agggccgggg cagcggttcg gaaagcgaat cggcaatcgc gggatcgacc agcgagatca
    22621 gcgctccggc ctgcctcagc gattcggctt cctcgcggca ttcggagcag gaccggaggt
    22681 gggcgtcgac cgcggccttt tcatcggggt cgaggtggcc aaccgcccag gcaccgaggc
    22741 tttcacggat ctgtcggtgc tcaggactca tagccaagtt cctccatcgc gtcccgcatc
    22801 gcccgcagcc cgtagtagac ccggctcttg atcgtgccct cggggatgcc gagcgccgcc
    22861 gcgacctcgg catagggccg accccgcaga caggtctcga ccacgatctg gcggtgcctt
    22921 tcaccgatcc ggcggagtgc ctcctcgacc tgccaggcca gcatcgcccg ctcgaacggc
    22981 tggtcggttg aagggatccg gtcggcggat tcctcaagcg gagcgccggc cggccgtcgg
    23041 ccatcggacc gaccgaggtc ggcgatcacg ttgcggagga tcgcgaagag ccaggtcctt
    23101 tccgagcccc gttccgggtc gtagctcgcg cggctgcgcc aggccttgag gaaacactcc
    23161 tggacggctt cctccgcccg gtcccggtcg ccgagtgaac ggcaggcgaa gccgaagagc
    23221 tcaccggagt gagcccggta ggcctcgcgc acctgttcac ccggcatcgt cactggtcgg
    23281 ttgcctcctg gtcagaaact aactgtccgg tccgccgtct cagggctttt cgccactcgg
    23341 ggtcaaggcg taccactcgg caccgaactg gtcgatgtca ttgcccttcg cctggtccgg
    23401 cttctgatcc ccggcgtaga ggtaaagcgg ccagccggcg taggtcacct gggtcgagcc
    23461 gtcggtccgc ccggttgtgc cgagcttgcc cgccacggcg ccattcttgg cgaccggatc
    23521 tccgtcggtg gccaggggcg gccatgcctc ggcgcagccg ccgtagcagg ccgactttcc
    23581 gcccttgtcc ttttcgaaca tgtaaagggt catccccttg tcgtcgacca gcaccttgcc
    23641 gaggtcaccg acgttcgccg ccgaaacgac cgccgcaccg gcagccatcg aaccgcccgc
    23701 cgcgccggaa ccgttcgaac cgtaatccga tttgccggtc gaatccgccg actgtgatcc
    23761 ggcctcgttg ccgtaaccgg tcccggcgct cgatccattg tcggacgagc cgtaagagcc
    23821 gccggaatcc gagtcgccgc ccccgcagcc ggccaggatc agggttccgg ccagaacgac
    23881 cgccaaagtc gccaggcccc tgcgatccaa aatccatgaa gagttcatgt gaggggatac
    23941 gacccggccc cgaaaaggtt caccggctgc ggccaggatc gtagtctgcg gaaccaggaa
    24001 cgcgtaccgc tagcgcccag cgaggaggca gaatggagcg ctatatggcc actttcgcct
    24061 tctcgatcgg gtccggttcg ttttttcgga atttgcgggg tgaaacccgg ccttgagcgc
    24121 ggccctgtac ccttgtgggg ataaggaacg acagttctaa acagatggcc gccaaccggg
    24181 cggccggagg aaaaacgaat aacccaatga gtattttcga aacgactcgc gttgagacgc
    24241 gggtcggcga cgccgacatc tccttcgaga ccggcaagct cgccaagcag gccagcggtt
    24301 ccgtcgtagt aaagagcggc gacaccatgg tcctctcgac cgccaccatg ggtggtctgc
    24361 gcgacgtgga cttcctgccc ctgaccgtcg acgtcgaaga gcgcatgtac gccgcgggca
    24421 agatccccgg ctccttcttc cgtcgcgaag gacgcgccgg cgagaaggcc accctgaccg
    24481 cccgcatgat cgaccggccg atccggccgc tcttccccaa gggctggcac tacgagaccc
    24541 agctggtcac gatcccgatg tcggtcgacc aggagcatcc ctacgacatc ctcgccatga
    24601 acggtgcttc cgccgccctg gcgatttccg agattccggt cgccaagcac gtcggtgcgg
    24661 tccgcatcgg caagctcgac ggcgacttcg tgatcaaccc gaacgaggaa gaccacgagc
    24721 tgctcgacct cgacctgatc gtcgccggca ccgacgaggc catcctgatg gtcgaggccg
    24781 gtgccaacgg cgtgaccgag gccgacatcc tcgacgcgct tgacatcgcc cacggcgaga
    24841 tcaagaagat caccgccacg atcgaagaac tccgccagaa gatcggcaag gagaagctgg
    24901 tcatcgagga gccgacgatc gacgagagcc tcctctccga catccggaat tcgcacggcc
    24961 aggccctggt cgacgccatc gccaccaccg gcaagctcga gcgctacgaa gcgatcgacc
    25021 aggtcaagga agcggtcgtc gagcagtact ccgccggcat cgccgacgag ttcgaggccg
    25081 atgcgcgcaa gtcagaggtc ggcaaggcct tcgacgcaat cgagaaggac acgatccgca
    25141 aggcgatcgc cgtcgacaag aagcgccccg acggccgcgc ccaggacgag atccgtccga
    25201 tcgagtgcga ggtcgatgtt tcgccccgcg ttcacggttc ggctctcttc acccgtggtg
    25261 agacccagat cctcggcaac gtcacgctcg gcaccacccg catggacatg cgggtcgacg
    25321 gtctcgggct ggagacgacc aagaccttct ggcatcacta caacttcccg ccgttctcgg
    25381 tgggcgaggc cggcttcatg cgcggtccga agcgccgcga catcggtcac ggcgccctcg
    25441 ccgagcgcgc cctggccccg agcgtcccgg accaggaaga gttcccgtac gtgatccgcg
    25501 ccgtctccga gacgctggag tcgaacggct cctcgtcgat gggttccgtc tgcgcctcct
    25561 cgatggccat gcaggccgcc ggcatcccgg tgaccgctcc ggtcgccggc gtggcgatgg
    25621 gcctgatcaa ggaaggcgac gactacatcg tcctgaccga catcgccggt gtggaagacc
    25681 accttggtga catggacttc aaggtcgccg gcaccgcgga cggcatcaac gccctccaga
    25741 tggacatcaa gatcaccgga gtcaccttcg agatcctgac cgacgccctc gagcaggccc
    25801 gcaagggtcg actcttcatc ctcggcaaga tggccgaagc gatcgacggc ccgcgcgagc
    25861 agctttcgcc gcatgccccg cgcatcgagt cgatcaagat cgaccaggag aagatcggcg
    25921 ccgtgatcgg caagggtggc gaaaccatcc gcggtctctg cgaggagttc gaggccgaga
    25981 tcgacgtcga agacgacggc acgatccgga tctacgcccc gaccggcacc caggtcgagg
    26041 acgcggtcgc ccgcatccag tcgatgacca aggaagccga agtcggcgac cgctacaccg
    26101 gcgccaaggt cgtgaagacc accaccttcg gtgccttcgt cgaactgacc aagggcaccg
    26161 acggcctgct ccacatctcc aacgtcaagc ccggtggccg ggtcgagacg gtcgaggagg
    26221 tgctcgagca gggccaggag atcgacgtca ccgtggccga agtcgaccgc gagcgcggac
    26281 ggatcggcct gcgcatgacc gacgaccccg cgattgccgg caagagcccc gaggagatcg
    26341 cagccctcgc cgcaaacagc ggtggcggcg gtggccgtcg cggcggtgac cgtgaaggcg
    26401 gacgccgtcg tcgcgaccgt ggcgaccggg accgccgcta ggcagcgtcg gaatctccag
    26461 cgccacatga gcactgacga accgcgcctt tcagagaccg cagccggcgt ccgggtgatc
    26521 accgaggtag ttccctcggt gcgctcggtc gccctcggcc tctgggtgag aacaggttca
    26581 cgtgacgaga ccctcgccca ggcgggggtc tctcactttc tggagcacct gctcttcaag
    26641 ggcacaccga actactcggc gatcgagatc tccgagcgct tcgacgggct cggtgcctcc
    26701 ttcaacgccg cgaccggcaa ggagacgacc cacctccacg cccggttcct cgacgaacac
    26761 accgccgacg tcttctcgct gcttggcgag atgctgctcg aatcgacttt ccccgacatc
    26821 gattccgaaa gggatgtggt gctcgaggag atcgcgatgt acgaggacga gccctcggac
    26881 cgggttcacg actacctggc cgatgcgatc ttcggcgaca cgccgctcgg acgccgcatc
    26941 ctcggccgct ccgaagtcat ctccgcgatc ccggtcccgg acatcgcgtc ctaccaccgg
    27001 gcccgctact cggccaacaa catcgtcgtc gcggcggcag gcaacgtcga tcacgacgag
    27061 atcctggcca tggccgagaa actggccccg ggcaccggaa gcggggagga gaccctcgtc
    27121 ccgcaggtgc cggacccggc cgggaggctt gaattcgcgg tcaaggacac cgagcagtac
    27181 cacgtctgca tcggcgcacc cgggatcaac cgggccgacg agcggcgctt cggacttgcc
    27241 gtgctcgact cgatcctagg cgggtcgagt tcctcgcggc tcttcatcga agtacgggag
    27301 aagcgcggcc tggcctactc ggtcggcacc tattccgagc agttcaccga caccggcgtg
    27361 gtcgccacat acgtaggcac ccgcgaggac aacgtcgagg aagcctgcga agtgatcggc
    27421 accgagctca agcggatcca gtgtgaaccg gtcacggccg aggaacttga ccgggccaag
    27481 gaacacgtca agggccgtct ggtgctctct ggtgaatcga ccgcggcccg gatg
//
LOCUS       DAOQKN010000010        26542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_747_length_26542_cov_6.136369, whole genome shotgun sequence.
ACCESSION   DAOQKN010000010 DAOQKN010000000
VERSION     DAOQKN010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 26542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26542
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_747_length_26542_cov_6.136369"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(213..4232)
                     /locus_tag="PLJ64_01435"
     CDS             complement(213..4232)
                     /locus_tag="PLJ64_01435"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNC92186.1"
                     /translation="MNRVFAVLAFVVAALLIGVAPASADPAPDLPAGPVLATDDWPND
                     PNFARCELQDPVSGCQDSEQWNLYGPMDGLCLAPGGAVFDQPRPDGGLPCWARNATDP
                     ESSSGINMTGAWAQGNIGRDDVLVAYVEGGVNYSANGIKDGLDSIYLNAGELPYPQGW
                     DGQDLGRYDFDGNGEFDIRDYAKDPRVNPACVSPTTQFTTYQEGTTRGCADDGTHEYL
                     HSVNIGGPLTPYLSPEDLIAVFGHCKITDHRIESCPADARIDNDGNGYPNDVSGWNAY
                     QNTNDPQSEDFNYGHAPGLIGEMGGEADNGYAGVGNCRNCRVVPIKSGAESVGKADGL
                     APAITYAADINVTAMSTVVVSYAYSSALKAAVKHAVDKGILMSMDSNDFDSMDHTDGH
                     LFDDVFPGNGLAQDIEGKNAQTFRARSNVTSYGTHSIFSGGESTTSGATPFQAAFLAM
                     TQSAALNAVDENIIPRKLTPNEIKQVLINTASEILPQRDTPDVANQWPGNPDSKTDST
                     HTAWSTQYGYGRVNIGKATKLIMDGRVPPTADIDSPSWYKYVDPTKGGKLQVKGKVAP
                     SAWGSEGLEWTLEWALGANPSDDDFKALSTGKAAKSGVLGTLDLKDIPASYAKAAPAE
                     NVMPPDGPEQYTVTFRLRARDGNGLKGEDRRSIGARADKDLVNGFPKAIGTEMSAAPS
                     FVDLGGKRQLELVFGTMDGSVSALQPDGSQAPGFPVYTWKLKDIDPRNPQNFDSEAYS
                     SVQALRDARDPISGIAVGDLDGNGAQSIVATTTSGWVYVWNSSGKLREGFPVHGDDQY
                     NTLPVPTPWSGGYHARSPIRGNWSAPTLGNLQGNGKLSILMSSYDGHVYAWQPNGDPT
                     PGWPVEVKLPDSIKSTIPANSYIHDPKLMMAPAVGDVLGTGTDQVFLPGFDCDDAANK
                     TFAYGIQSDGNNNPGGPYLPGWPIKMGSIGGCYSQSIDFVQEGANAASIGDFDNSGQL
                     RIAITPVAGSPVIVNGDGSLYKGMTPGCTSAPCSGYDPYYGSDSITVGVTSQSAIGDL
                     TGDGMPEFMQPTAGGATLSGALNDVGQAALAHVFDQAWNPTTGAVLDNFPTDQDGFPF
                     YASPVVANLTDDGNRSMITANDSYWIHARGPDGQEAPGFPKWTGQWTSFGGAVGDPKF
                     DGQQYLAYGTREGQVFLWKVGGKPSMNDQWWHYRHDEHNSGRYGNDTRPPAGVKFVVK
                     RSKKSAQVTWKASGDDGVNGDKVAVLQVYKSNRKISLSQLGSKVKSPAIAKPNGQQKV
                     NLKLAPNKSLYVAVRQQDADGNWSSLSRFKIKPFKSYNLKKQKKICRKKFRGSKRKQC
                     IRKAEKKAKAVAKQNTQRS"
     gene            complement(4258..5367)
                     /locus_tag="PLJ64_01440"
     CDS             complement(4258..5367)
                     /locus_tag="PLJ64_01440"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNC92187.1"
                     /translation="MDAESNGRPSEEETVEETVASPSLTERLVGAGTGGARRMAGATG
                     LDQAMENAVEEAIVRAIESEAVERSMTRIINGPLIENAVRDAMKSPAVEQALMEVVDS
                     ELVDRLWARILDSNETQRLIERIAEAPEVRAAIASQSLGLVDDLGRGAARVTRAIDFA
                     LERLARRIFFRSRREVPTDRVGVATRALALVIDGVAVNLVMISSTAVLGMIASVLGID
                     LGNLGGAAYAVGGAVWLGLGSLYLFAFWAFSGQTLGMRFFDIRIEHEGKWVIGPRRAF
                     RRLVGFWLSAIPFCLGFLGVLLRLDRRGFHDRLGQTMVYYINPAGPNQPHLEPVLQPG
                     MDLMDDLVPDEPLISAEKPIDSPQGLPREVRGPGK"
     gene            complement(5398..6156)
                     /locus_tag="PLJ64_01445"
     CDS             complement(5398..6156)
                     /locus_tag="PLJ64_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92188.1"
                     /translation="MPRSGWQLLRDGFPDRPEMGPALSRTLLEQVAAGQRPATVRLSR
                     PGRVVAFGRRDVVSPGYADAVAAARAGGFPGMERLSGGRAAAFTEGALSLTFTLPDPK
                     PAERTVQRFTEWSGLVRDAFIDLGVDTRIGAVPDEYCPGDFSVNAGGRVKLAGAGQRM
                     IRGAAHLGFVILVSDSALVAETLGPVYAALGLDFNPGTVGSIEDARPGTGLEDVEQAL
                     LGRLESAVGLQPAEIDPVTLEMTRSRSGQFRSNP"
     gene            6331..6726
                     /locus_tag="PLJ64_01450"
     CDS             6331..6726
                     /locus_tag="PLJ64_01450"
                     /inference="COORDINATES: protein motif:HMM:NF018447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HNC92189.1"
                     /translation="MTSETPVGVAFQIGDLVQQSRAEAAERIVERQQAEVQRKLEARQ
                     QQEKFGDLPGSVTMETLEAIADCESGGDPKIVSSSGLYFGKYQFSPGTWESVGGKGLP
                     SDAPEAEQDYRAALLYERSGPGQWPVCGQ"
     gene            6859..7671
                     /locus_tag="PLJ64_01455"
     CDS             6859..7671
                     /locus_tag="PLJ64_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92190.1"
                     /translation="MSSGSTRRIVERSHKVTAGIDGLDRISALIMSEAEFARDQGAGR
                     VEVIAVPGLRGSRLIRLLDRVADAVGAGPIRIPTRRDWTAATFLGVTVPAGDSLPDPV
                     AVAVIGETAIGFAVGRPGEIPEWNGSRPVGASTMTRKARFQDPPRPGQIEAAVIGASR
                     GIASMFPPPTERVLVVSSLAPVVERLCGPRIGAAEARRGLDAILGQTSDDISAWFGAE
                     APLARHLPGIIVGHAALAEGLGVRVEPALCDLAASRYWLAEREEPVAGRESS"
     gene            7668..8549
                     /locus_tag="PLJ64_01460"
     CDS             7668..8549
                     /locus_tag="PLJ64_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNC92191.1"
                     /translation="MSDSGEIAEGEELAVGAPDPADQAPALHSRENVRSVRVKAPTRG
                     NRKLEKFLDAVNRDDEVKAWWYMAQTHAERLGMSDHSWVHMQIVLNIALRLTRLLDKG
                     GVHGAMVTDHGMSARDTEVVVAGGALLHDVGMSIHRADHEEYSLFLAREALPRLLDGI
                     YEEPEKSIVMAEVLHAIIGHRRRGQPYTVEAGIVRVADALDMAEGRTRVPLESGQEGI
                     HSISAAAIDRVNISAGENRPVRLEIELNNSAGIFQVDDLLATKIRDTPLAGHIEVVAE
                     VKGESEKRLLSGFRLGA"
     gene            8546..9598
                     /locus_tag="PLJ64_01465"
     CDS             8546..9598
                     /locus_tag="PLJ64_01465"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HNC92192.1"
                     /translation="MSTSLVILREQAGHETGSHPENSRRFPGVLNRLGEDSQRHGLPI
                     LESREATVEDLLRAHTESHVENVRVAAENGPTWLDGDTVASPLSYEVARRSAGAALVA
                     VDAVNGKAAEGTATTSSFAIIRPPGHHATADRAMGFCLFSNAAIAARYAREALGIGRV
                     AVLDWDVHHGNGTQDILYDDPGVLFISFHQWPLYPGSGWLDETGVGAGRGFTVNLPMP
                     PGCGDREHIEGFQAIAGPILDQFDPGLLIVSAGQDGHVADQLASQNLTAAGYHALASA
                     SADFASRKGIGLVALHEGGYNPNTLPLLDHAVYAGLGGFTPDLTGDTPAPELEETGWP
                     ERLAQIRRAQSVFWAV"
     gene            complement(9706..9954)
                     /locus_tag="PLJ64_01470"
     CDS             complement(9706..9954)
                     /locus_tag="PLJ64_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92193.1"
                     /translation="MNRVRNSLVALLTALFVLALPAYAAAADGVGTAGRVDDRYITFF
                     CFGLIAFFAILVTVLSLIQGRLDAKKDQRRHDLDRFNS"
     gene            10351..11145
                     /locus_tag="PLJ64_01475"
     CDS             10351..11145
                     /locus_tag="PLJ64_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC92194.1"
                     /translation="MNAGRAPMRVAVIDTDSGFIRVLSNRMDAAGWQYRVLASGVPPE
                     ELVAMKINAVVFDPDVIGEGGWEYLERICGMLPDLGVIVCANGGTVSQRVRGLRIGAD
                     DWVNKPCHPEEVIARIEAVVRRRRRHSNEQDAGPLVVGEIEIRPDQFQAFIAGQSLDL
                     TRREFELIQLLAHNEGKVLDRESIYQRVWGYAMAHGDRSVDVFIRKLRTKLERRSPAY
                     TYIHTHFGVGYRFAAESSISAAEATAKVSSDRATDDAGAAAPGVPV"
     gene            11328..12011
                     /gene="phoU"
                     /locus_tag="PLJ64_01480"
     CDS             11328..12011
                     /gene="phoU"
                     /locus_tag="PLJ64_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNC92195.1"
                     /translation="MTEKIRIQYQEELDHLEQQALGAFDLVDRALGRTLEAIENQDVE
                     LAELVAADDDRIDGRYLEIHQGLITLLATQSPVATDLRLISALLHVIKNAERMGDQCV
                     NICKLIPIVGHEPPSDESMIRLILEMGVQASSQIIQARQAFADRNETAAQDLVRQDDL
                     IDRLNRDCFTLAIEIGDEPDRREWAMTMLLAARAIERIGDNAVDVGEQVAFVVTGLFR
                     EFEDASHPI"
     gene            12024..12377
                     /locus_tag="PLJ64_01485"
     CDS             12024..12377
                     /locus_tag="PLJ64_01485"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNC92196.1"
                     /translation="MEAAKSQTGGSAGHALRAGPLEVRLDQRTVLADGRGIILTVREF
                     HLLAVLASRPEQVVSREEVYDLVWGGAMPENDRSVDVYVSKLRHKLETALPHWSFIQT
                     HIGFGYCFSPRPLNP"
     gene            12518..13606
                     /gene="pstS"
                     /locus_tag="PLJ64_01490"
     CDS             12518..13606
                     /gene="pstS"
                     /locus_tag="PLJ64_01490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HNC92197.1"
                     /translation="MKKWLIAIPLTLALALGVAACGSSDSDSGSSGSSGDAVSGEIAG
                     AGASSQEAAMAAWVAGFQENNPDANIAYDPVGSGGGREQFIAGGTDFGGTDTPMDETE
                     LADVAKRCKPGEYIEIPVYISPIAVPYNVEGVDDLNLSASTLARIFAGDITKWNDQAI
                     AAENPDASLPDQDIVVVHRSDESGTTENFTDYLHQAAPKDWTDEPDGVWPLQGGEAAN
                     GTSGVISAVKSGSGTIGYADLSQVGDLGTVNVKVGSDFVAPSPEAAVKIFDESKRIGG
                     EGKYIFAYELNRTSTAQGLYPISLVSYEMACTTYDSADTAKLVKAFYSYIISDEGQQA
                     AASNAGSAPIPASLSKQITPAVEAIQTS"
     gene            13708..14661
                     /gene="pstC"
                     /locus_tag="PLJ64_01495"
     CDS             13708..14661
                     /gene="pstC"
                     /locus_tag="PLJ64_01495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNC92198.1"
                     /translation="MNAGTVSNSETPPSGRAHRRFGDRAFSGLATAAGLAILVVLAGV
                     AAFLVSEAWPAITAPVADLPDGKSLLHYIWPLVFGTVLSSLIAILIAIPMAIAVALFT
                     SHLAPKPIAMSVSYLTDLLAAIPSIIYGIWGIAVLGPASVPVTQWLSDHFGFIPFFDG
                     PASTTGRTMLVAGVVLALMILPIISAVAREVFQQTPKGLQESALALGATRWEMIRMVV
                     LPFGRSAVVAGSMLGLGRALGETMAVTIILSVSGVITFDLISSENPSTIAANIALQFP
                     ESSGIDVNVLIATGLVLFAITLAVNMAARAVIGRREAAGGR"
     gene            14672..15706
                     /gene="pstA"
                     /locus_tag="PLJ64_01500"
     CDS             14672..15706
                     /gene="pstA"
                     /locus_tag="PLJ64_01500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNC92199.1"
                     /translation="MPLGEGSGVLPRRAGLLVFGISAVASALLCLVLGFSPFVWLIFT
                     GLFSLVTITVWSLAVEGRRRALDRVMTVVIVEIFVLALVPLISLVYTVVSEGLARFDV
                     QFLTEDMRGVVGEGGGAFHAAMGTFIITAFATVAAVPVGVLAAIYLVEYGKGRLKRSI
                     TFFVDVMMGIPSIVAGLFAFALFALFFGPGIRLGIMGSVALMVLMIPIVIRSAEEMLR
                     VVPNSLREASYALGVPKWLTVLRVVLPTALAGIVTGVMLAVARVIGETAPLLVTTGSI
                     DTTNLDPFDGRMANLPVYVYEQYKFPGVPPEAFIERAWAGALLLILIVMVLFLAARLI
                     SRRYGVEIRR"
     gene            15817..16596
                     /gene="pstB"
                     /locus_tag="PLJ64_01505"
     CDS             15817..16596
                     /gene="pstB"
                     /locus_tag="PLJ64_01505"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNC92200.1"
                     /translation="MAKRIDAEDLNIYYGSFLAVKDCTVSIHPNKVTALIGSSGCGKS
                     TFLRSLNRMHELIDGARVEGRVLLDGMDIYSPSMDPVLVRQQIGMVFQQPNPFPTMSI
                     YENTAAGLRLNRRRLKKTEIDGIVERSLRGAHLWDEVKDRLEKPASGLSGGQQQRLCI
                     ARATAIEPEVLLMDEPCSALDPIATLAIEDLINELKKRYTIVIVTHNMQQAARASDYT
                     GFFNIEKSGDPGVLVEIDETEKIFSNPSLQETEDYVSGRFG"
     gene            16676..17584
                     /locus_tag="PLJ64_01510"
     CDS             16676..17584
                     /locus_tag="PLJ64_01510"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92201.1"
                     /translation="MLCAAIDIGSNTTRILVAEPDEGKLKKVMEQRAYTRIGQARLDD
                     GTIPAEKVAEVAEVVDTQVKLARELGAGTFRAVATAALRDAPNGKEAAAEIEAVAGIP
                     VHVVSENEEGRLAFLGATKALGYQVEGRVAVVDVGGGSTEIIIGTADGGVEQVRSWPI
                     GSGQLAEELISSDPPSASEIRRVRDRVDEVFAEVQITQPEQAVAVGGSATSLRKIVGA
                     RLEYETLERSIRVLSGDEALEVGRQFELDPIRVRMLATGVLILEKVSELLGQDLQIGK
                     GGIREGIVLDMLNGGIDVSEPALTPG"
     gene            17613..18617
                     /locus_tag="PLJ64_01515"
     CDS             17613..18617
                     /locus_tag="PLJ64_01515"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HNC92202.1"
                     /translation="MSTESDPRFRLAASQVLEERATQISVLAKGVLDVTETGAADELW
                     LASRRLRAALEVFRSCFGRAQYREGRAEARRISRAAGARRDIDVVIAAYESVGEETAP
                     AESEGIDRVISSLRREQASANRALAQVVHGRRLQAFRVRIEEIADSAVEADAADRAHD
                     YRPVETLTPEAIALISKRLARLRKEAVDSLDPEGVDAQHRLRVAAERLRYSLELTGDA
                     LGSQAHTARRAARGLQEVLGEMRDCDLAVPPARQAITDLEAEDVKTIIDRARGSRDLD
                     PILVQAAPNRAAYRGLELAVVHMTARRQMLYERFKRLWHEQSRQGVWVALETSLKVES
                     "
     gene            18620..20713
                     /gene="ppk1"
                     /locus_tag="PLJ64_01520"
     CDS             18620..20713
                     /gene="ppk1"
                     /locus_tag="PLJ64_01520"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HNC92203.1"
                     /translation="METTPDPSWFFNRELSWLDFNQRVLELAEDDSAPLLERVKFCAI
                     FASNLDEFFMVRVAGLWDQVDAGIDARGPDGLSPREQIQAIRSRSLELDRRLTRVFER
                     KLKPQLTENGIKVATLADLNGTERAELDRIFDQQVFPALTPLVIGLGRPFPYISNLSL
                     SIAVLLRDRETSTRVLARIKVPKELLGRFIELSSEKERVLVPLEEVIAANLDQLFPGV
                     EVLDHSYFRVTRDADFTVSDEADDLLEAVQEEVRRRRFGEVVRLEVADGMNPELKELL
                     VELLRLQQNEVFEAGGLLDLTDLWDVVKLGGFQELRDKPWAPVTQPRLQGEDGDPPDI
                     FAAIRQRDVLVHHPYDSFSSSVERFIAQAVSDPDVLAIKQTVYRTSEDSPLVSALIKA
                     SERGKQVVCMLELKARFDEEANIEWAKRLEEVGVHVVYGIPGLKTHIKALLVVRREGD
                     QVRNYVHIGTGNYHSTTARLYTDLGLFTADPEIGADVAEMFNLLTGYSRPADYRRVLV
                     SPHSMRSSILAEIEATVAAHKAGREAKILMKMNSLVDRTCIEALYRASQAGVEVEINV
                     RGICCLRPGVPELSENIRVVSLVGRFLEHSRVYAFQRGGDWNIFIGSADLMPRNLDAR
                     VELVTPVEDEIARDQILDVMERCFSDNSNSWVLDSSGNWSRLSPEGGERRNAQEELME
                     RHQTLAATALPQANG"
     gene            20777..21394
                     /locus_tag="PLJ64_01525"
     CDS             20777..21394
                     /locus_tag="PLJ64_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNC92204.1"
                     /translation="MRFSLLPRDNTFFDLFNEAGQNTLRAATKLNDMMAAWPDDNGLG
                     REILIIEQEGDRITHDIINRLNSTFVTPIDREDIYGLATKLDDVVDYIEEAADFLALY
                     QIEAPMDQAQDLTRILVGCCEQLSLGLENLPSFRDLDQYWIEIHRLENDGDRVSRDAV
                     ASLFSNGIDPMVVIRWKDIFAVLEKAIDATETSAHILEGIVIKNS"
     gene            21399..22520
                     /locus_tag="PLJ64_01530"
     CDS             21399..22520
                     /locus_tag="PLJ64_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNC92205.1"
                     /translation="MDLHLILVIVVVSALAFDFTNGFHDTANVVATSISTGAASPKAA
                     IAFAAILNFIGAFISIEVAATIASDVVDQSAIQGAGSLNVVFAGLVGAIAWNLITWYF
                     GLPSSSSHALIGGIVGAALADSGSQAIETAGLVGKVLVPAIVAPILAFVVAALAIGLI
                     YRITARLRPGPVTRGYRLGQIASGGLLALAHGTNDAQKTMGVITLALIADGALPEGAS
                     PPTWVIVASATAIALGTYSGGWRIIKTTGMRIIKMDAAQGFSAQGAGAAVIMASSHFG
                     FPLSTTHVINGGVMGSGVAKRVSAVRWGVAGNIVTAWILTLPAAAAIGALCFGITEIF
                     GDGAAGPVIISAIAVVLIVIAFVRRSRQTEVVDVQTEVA"
     gene            22524..22769
                     /locus_tag="PLJ64_01535"
     CDS             22524..22769
                     /locus_tag="PLJ64_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92206.1"
                     /translation="MDLFASVVAWKELGDTVLYAFVGAMAVSLAASLGIWGATKYVDF
                     NQDGRGVVAGMALAVGAFGLVATIGIIALGIYLMVSK"
     gene            22810..23160
                     /locus_tag="PLJ64_01540"
     CDS             22810..23160
                     /locus_tag="PLJ64_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92207.1"
                     /translation="MTIARNIAIIALLAAGVAFLPNGGNVADAVLTTITMAFLAGLAW
                     TVYRLTYEFRSGLLALEGSRRVILYSCFGLIVLMIAGADKMFSTGFGTMTWLLLMASG
                     LVGVWLIVSEARSY"
     gene            23157..24008
                     /locus_tag="PLJ64_01545"
     CDS             23157..24008
                     /locus_tag="PLJ64_01545"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNC92208.1"
                     /translation="MNFRVLSWNLFHGRDFPPAPGKEDPGVPPGDLLWRITGRPIPGK
                     KHVRLNRDLFHEFAALLAAAKWDIALLQEVPPRWAHRLAEATGAEARTALTSRNWLRP
                     VMSPVARFRPQLAGSWEGGCNLILVRGATPGLDKSRPGAHIREFRKSTLTWFPERRVM
                     SMVELGCGLCVANLHASTGERAAGDVMRAARLASGRAGDRPLVLGGDFNARPRSSDVF
                     DRLEGEFGLAGTTAPGSIDHLLSRGVEVLEQPEAWPPARRDVPDPETGLMVRLSDHAP
                     VLARIRV"
     gene            24078..24437
                     /locus_tag="PLJ64_01550"
     CDS             24078..24437
                     /locus_tag="PLJ64_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92209.1"
                     /translation="MDADREKAGSGGIGSRRGSVSLELIGSLPIILLAVLVAAQIAIA
                     GAALWSAGVAARAGARAVLTGNSPRGAAEHALPSVLQGGLKVSQEDGVKVGVRVPGLL
                     PGFPETHVYGSSSLGDG"
     gene            24437..24769
                     /locus_tag="PLJ64_01555"
     CDS             24437..24769
                     /locus_tag="PLJ64_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92210.1"
                     /translation="MNSGRSGQATVEAVCGIAALMLAGLLCFQLLATGYTATIADGAA
                     EAGAVALIRGEPVEPAVLEALPGWARDRVSVSRVGSRVKVRLRPPSLFSSMSEKLAVS
                     AEAGGRPG"
     gene            24766..>26542
                     /locus_tag="PLJ64_01560"
     CDS             24766..>26542
                     /locus_tag="PLJ64_01560"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HNC92211.1"
                     /translation="MSEHLLIGSIGDSRGAERLAAERVLESAAGRVGVVLETGRERPR
                     PASLLAPGEAKELESELLEALPELEPKARGSLVYLSAAQSERLAGEVISLREIVDERP
                     LAVIAGAGRYRELLELGWDRVVIRSDEAGQDAPEVDAPVSGDAVAGPGALTGLVVEEA
                     GEFADRVELVRGAAGKLWPAGGRLLGRLRSTGGQATPLVLGCVFALVLATVAVVAISG
                     AVTGKARAQRAADLSALSAVRSMKDDLPRLLAPPRLPNGLPNPAHMPKQVYLARARLA
                     AIGAATSNGASPLAVSVRFPDSASYAPVRARVSIATSVRPGTGGGAHRAPPNWAEARV
                     SVPVSMGSVPAMASGGGYSGPLAERQGHGMRPDVAAAFDAMVASAAAAGVSLTINSAF
                     RSDAEQAALFAANPDPTWVAPPGQSLHRCATELDLGASDGYSWLAANAGRFGFVQRYS
                     WEAWHYGFVAGPAPCSDAGNSVGMDGGDRSAALSRAVPSFTPGWVRPLVLRAAMKWNV
                     SAALLAAQLFTESGFDPNAGNGIAFGIAAFTPAAAAEYGLDDPFDPAAAIMAQAHLMS
                     DLLRQFGSPALALAAYNAGPGAVGSC"
BASE COUNT         4330 a         8706 c         8869 g         4637 t
ORIGIN      
        1 cgggtggccc tcccaaagtg gcttcccgat tactgctccg gtcagcgagc aggtgaggac
       61 aagcgcgacg gtggcgccat ctttgggagg gccacccggc cccacggcgt caccaccgag
      121 tgccggttcc ggggcatgaa ccgatccgcc gggaatcaaa aggtgccgca ggttttgcac
      181 ccatatcggg ccagaacctg cggcacctcc cgctaggacc gctgggtgtt ctgcttcgct
      241 accgctttgg ccttcttctc ggccttgcgg atgcattgct tgcgcttgct gccacggaac
      301 ttcttgcggc agatcttctt ctgcttcttc aggttgtagc tcttgaaggg cttgatcttg
      361 aagcggctga gcgatgacca gttcccgtcc gcgtcctgct ggcggacggc gacgtagagg
      421 ctcttgttcg gagccagctt gaggttgacc ttctgctggc cgttcggctt ggcgatggcc
      481 ggcgacttga ccttgctgcc gagttgcgac aggctgatct tccggttcga cttgtagacc
      541 tggagcacgg cgaccttgtc gccatttacc ccatcgtcac cggaggcctt ccaggtgacc
      601 tgggccgact tcttcgagcg tttgaccacg aacttgacgc cggccggggg ccgggtgtca
      661 ttgccgtaac ggcccgagtt gtgctcgtcg tgtcggtagt gccaccactg gtcgttcatc
      721 gagggcttgc cgccgacctt ccagaggaag acctggcctt cccgcgtgcc gtaggccagg
      781 tactgctggc cgtcgaactt cgggtcaccg accgcaccgc cgaacgaggt ccactggccg
      841 gtccacttcg ggaaaccggg agcttcctgg ccgtccgggc cccgggcgtg gatccagtag
      901 gagtcattgg cagtgatcat cgagcggttg ccatcatcgg tcaggttggc aaccaccggt
      961 gaggcgtaga acgggaagcc gtcctggtcg gtgggaaagt tgtccagcac cgctccggtg
     1021 gtcggattcc aggcctggtc gaagacatgg gcgagagccg cctgcccgac atcgttgagg
     1081 gccccgctga gcgtggcacc accagccgtc ggctgcatga actcgggcat gccgtccccg
     1141 gtcagatcgc caatcgccga ctggctggtc acgccgacgg tgattgaatc cgagccatag
     1201 taagggtcgt agcccgagca gggggccgag gtgcagccgg gggtcatacc cttgtagagc
     1261 gacccgtcac cgttgacgat gaccggtgag ccggccaccg gagtgatcgc gatccggagc
     1321 tgcccggagt tgtcgaaatc accgatcgag gcagcgttgg ccccttcctg gacgaagtcg
     1381 atcgactgtg agtagcagcc tccgatcgaa cccatcttga tcggccagcc cggcaggtaa
     1441 ggtccgcccg ggttgttgtt gccgtcggac tggatgccgt aggcgaaggt cttgttcgct
     1501 gcatcgtcgc agtcgaatcc gggcaggaag acctggtcgg tgccggtgcc gagcacgtca
     1561 ccgacggccg gagccatcat cagcttcgga tcgtggatgt agctgttcgc cgggatcgtc
     1621 gacttgatcg aatccggcag cttgacctcg accggccagc ccggggtcgg atcgccattc
     1681 ggctgccagg catagacgtg accgtcatag ctggacatca ggatgctcag ctttccgttg
     1741 ccctggaggt tgccgagcgt cggtgccgac cagttgccgc ggatcgggga cctggcgtgg
     1801 tagccaccgg accagggggt cgggaccggc agcgtgttgt actgatcgtc cccgtgaacc
     1861 gggaaccctt ctctcagctt gccggaggag ttccagacgt agacccagcc gctggtcgtg
     1921 gtggcgacga tcgactgggc gccgttgcca tcgagatcac cgaccgcgat gccgctgatc
     1981 ggatcccgcg cgtcccggag cgcctgaacc gacgagtagg cctcactgtc gaaattctgc
     2041 gggttgcgcg gatcgatgtc cttcagcttc caggtgtaga cggggaagcc cggagcctgc
     2101 gaaccgtccg gctgcagagc gctgaccgag ccgtccatgg tgccgaagac gagctcgagc
     2161 tgacgcttgc ctccgaggtc gacgaagctc ggcgccgcgc tcatttcggt gccgatcgcc
     2221 ttcgggaacc cgttgaccag gtccttgtcg gcgcgggcgc cgatcgagcg gcggtcctcg
     2281 cccttgaggc cgttgccgtc acgggcccgg agccggaagg tcaccgtgta ctgctcgggg
     2341 ccgtccgggg gcatcacgtt ttcggccgga gccgccttgg cgtaggaggc cgggatgtcc
     2401 ttcaggtcga gcgtgccgag cacaccggac ttggcggcct tgccggttga aagcgccttg
     2461 aaatcatcat cggagggatt ggcgccgagg gcccattcga gggtccattc gagcccttcc
     2521 gaaccccagg cggaaggtgc gaccttgccc ttcacctgga gcttgccgcc cttggtcgga
     2581 tcaacgtact tgtaccagga gggcgaatcg atgtcggcgg tcgggggaac ccggccgtcc
     2641 atgatcagct tggtcgcctt gccgatgttg acccggccgt agccgtactg ggtcgaccag
     2701 gcggtgtggg ttgaatcggt cttcgagtcc gggtttccgg gccactggtt ggccacgtcc
     2761 ggggtgtccc gctgggggag gatctcgctg gccgtgttga tcagcacctg cttgatctcg
     2821 ttcggggtca gcttgcgggg gatgatgttt tcgtcgaccg cgttcagggc ggctgactgg
     2881 gtcatcgcga ggaatgccgc ctggaagggg gtggcgcccg aagtcgtgga ctcgcctccg
     2941 gagaagatcg agtgggtgcc gtaactggtc acgttcgagc gggcccggaa ggtctgggcg
     3001 ttcttccctt cgatgtcctg ggcgagcccg ttgccgggga agacgtcatc gaagagatgg
     3061 ccgtcggtgt ggtccatcga gtcgaagtcg tttgagtcca tcgacatcag gatgcccttg
     3121 tcaaccgcat gcttgaccgc ggccttgagc gcgctcgaat aggcgtagga caccaccacg
     3181 gtggacatcg ccgtcacgtt gatgtcagct gcgtaggtga tcgccggagc gagaccgtcc
     3241 gccttgccga cagattcggc cccggatttg atcgggacca cgcggcagtt gcggcagttg
     3301 ccgacgccgg cgtagccatt gtctgcctcg ccacccatct cgccgatcag gccgggcgcg
     3361 tgaccgtagt tgaaatcttc ggactggggg tcgttggtgt tctggtaggc gttccagccc
     3421 gaaacgtcgt tcgggtagcc gttgccgtcg ttgtcgatcc tggcgtcagc cgggcaggat
     3481 tcgatccggt ggtcggtgat cttgcaatgg ccgaacacag cgatcaggtc ttcgggggac
     3541 agatagggag tgagcggacc gccgatgttg accgagtgga ggtactcgtg ggtgccgtcg
     3601 tcggcgcagc cgcgggtcgt gccctcctgg taggtggtga actgggtggt cggggaaacg
     3661 caggcggggt tgacccgcgg gtccttggcg tagtcgcgga tgtcgaactc gccgttaccg
     3721 tcgaagtcat agcggccgag gtcctggccg tcccagccct gggggtaggg aagctcgccg
     3781 gcgttgaggt agatcgagtc gagtccgtcc ttgatcccgt tggcgctgta gttgaccccg
     3841 ccctcgacgt aggcgaccag gacgtcgtca cgaccgatgt tgccctgggc ccaggcgccg
     3901 gtcatgttga tgccggaaga gctttcggga tcggtcgcgt tgcgggccca gcaagggagg
     3961 ccgccgtccg ggcggggctg gtcgaacacg gcgccgccgg gggcgaggca taggccgtcc
     4021 atcggtccgt acagattcca ctgctcgctg tcctggcagc cggaaaccgg atcctgcagt
     4081 tcgcagcgcg cgaagttcgg gtcattcggc cagtcgtccg tcgccagcac tggtccggct
     4141 ggcaggtcgg gtgcggggtc ggccgacgcg ggagcgaccc cgatcagcaa agcggccacc
     4201 acaaaggcaa gcacggcaaa gacgcggttc acgaacaaga cattcctccc ttggtactca
     4261 tttccctggt cccctcacct cccgaggcag accctgcggg gaatctatcg gcttttcggc
     4321 cgaaatcagt ggttcgtcag gtaccaggtc atccatcagg tccatccctg gttgcaaaac
     4381 cggctcgagg tggggttgat tcggtccggc cgggtttatg tagtagacca tcgtctggcc
     4441 cagccggtcg tggaatccgc gccggtcaag gcgcagcaga accccgagaa aaccgaggca
     4501 gaagggaatc gccgacagcc agaagccgac cagtctcctg aaagcccgtc gcggaccgat
     4561 cacccacttt ccctcgtgtt cgatccggat gtcgaagaac cgcatgccga gcgtctgtcc
     4621 ggaaaacgcc cagaaggcga agagatagag cgacccgagc cccagccaca cggcgccgcc
     4681 aaccgcgtat gcggcgccgc cgaggttgcc caggtcgatt cccaggaccg aagcgatcat
     4741 cccgagcacg gcggtcgagg aaatcatcac caggttgact gcgactccgt cgatcaccag
     4801 tgcgagggcg cgagttgcga cgccgacccg atcggtcgga acctcccgcc tcgaccggaa
     4861 gaagatgcgg cgggcgagcc gctcgagggc gaagtcgatc gcccgggtga cccgcgcggc
     4921 gccgcggccg aggtcatcga ccagtccgag gctctgcgag gcgatcgcgg cccggacctc
     4981 cggcgcctcc gcgattcgct cgatcagtcg ctgggtttcg ttgctgtcga ggatccgggc
     5041 ccagagccgg tcaaccagtt cggaatcgac cacctccatc aaggcctgct cgactgccgg
     5101 gctcttcatc gcatcccgca cggcgttttc gatcaggggc ccgttgatga tccgggtcat
     5161 cgagcgctcc acggcctcgc tttcgatcgc ccggacgatc gcctcctcga ccgcgttttc
     5221 catggcctgg tcgagtccgg tcgcaccggc catccgcctg gccccgccgg tcccggcccc
     5281 gaccagccgc tcggtcaggg agggtgacgc gaccgtctcc tcgactgtct cctcctccga
     5341 aggccgtccg ttcgattcgg cgtccatggc ttcagattag aggctgctga ccgggcatca
     5401 gggattcgag cgaaattgtc cggatcgtga cctggtcatc tcaagggtca ccggatcaat
     5461 ttcggccggt tgaagtccga ccgctgactc gagccgtccg agcagcgcct gctccacgtc
     5521 ttcgagtccg gttccgggcc gggcatcctc gatgctgccg accgtccccg ggttgaagtc
     5581 gagcccgagc gccgcgtaga ccgggcccag cgtttcggcg accagggccg agtccgagac
     5641 cagaatgacg aagccgaggt gagcggcgcc cctgatcatt cgctgtcccg ctccggccag
     5701 cttgacccgg ccgccggcgt tgacgctgaa atcgcccggg cagtattcgt ccggcaccgc
     5761 accgatcctg gtatcgacgc cgaggtcgat gaacgcgtcc cggaccaggc cggaccattc
     5821 ggtgaagcgc tggacggtcc gctcggccgg cttcggatcg ggcagggtga aggtgaggct
     5881 cagggcaccc tcggtgaagg cggcggcccg gccaccggac agcctttcca tgccggggaa
     5941 cccgcccgcc ctggcggcgg cgacggcatc cgcatatccg ggggagacca cgtcccgccg
     6001 gccgaaggcg accacccgcc cgggccggct caacctgacc gtggccgggc gctggccggc
     6061 tgccacctgc tccagcaggg tccgggaaag ggccggcccc atttccggcc gatccgggaa
     6121 accgtcgcgc aacagctgcc atccggaacg cggcatccgg tcgcttctga tatgagtgtt
     6181 tgctaacaaa agggagaaat catccttacg acgcgaaatt caatcctcat tctggcgctg
     6241 atcgctgccc tgattatcgc cgggaacctc gccttcgacc ggtcgaagga cggctccggg
     6301 acttccggtg ccgacatcgc ccttgccgcg gtgacctcgg aaacgccggt cggcgtcgcc
     6361 ttccagatcg gtgacctggt ccagcagtcc agggccgaag cggctgaacg gatcgtcgaa
     6421 aggcagcagg ccgaggtaca gcggaaactc gaagccaggc agcagcagga aaaattcggg
     6481 gacctcccgg ggtcggtcac catggagact ctcgaagcga tcgccgattg cgagtcgggc
     6541 ggtgatccga agatcgtttc ctccagcggc ctctacttcg gcaagtacca gttcagtccc
     6601 ggcacctggg aatcggtcgg tggcaagggt ctcccgtctg atgctccgga ggccgagcag
     6661 gattaccgtg ccgcgctcct gtacgagcgc tccgggcccg gccagtggcc ggtctgcggc
     6721 cagtagcggc tttccgtcca ataccggcgt ttacaggatt aattcggctt tcgggcacat
     6781 tgcctctagg atggcccggt cttgatcctt ggggcaattg aaatctcgct ccgcagtacc
     6841 cgcttttcgc tgtcggaagt gtcttccggt tcaacccgga ggatcgtcga gcgcagccac
     6901 aaggtcacgg ccggaatcga cggtctcgac cggatctcgg ccctgatcat gtccgaggcg
     6961 gagttcgccc gggaccaggg ggcggggagg gtcgaggtga tcgcggttcc cggtctccgc
     7021 ggttcccgcc tgattcgttt gctcgaccgg gttgccgacg ccgtcggagc cgggccgatc
     7081 cggatcccca cccgccggga ctggacagcg gcgaccttcc tcggtgtgac ggttcccgcc
     7141 ggggactcgt tgccggaccc ggtcgccgtc gccgtgatcg gggagacggc aatcggcttc
     7201 gcggtcggcc gcccgggaga gatcccggaa tggaacggct cacggccggt cggcgcctcg
     7261 accatgaccc gcaaggcccg gtttcaggat ccgccccggc ccggccagat cgaagcggcc
     7321 gtgatcggcg cctcccgcgg gatcgcctcg atgttcccgc ccccgaccga gcgggtgctg
     7381 gtcgtctcga gcctcgcccc cgtggtcgag cgactttgcg gcccccggat cggcgccgcg
     7441 gaggcccgcc ggggactgga cgcgattctc ggccagacaa gcgatgacat ttccgcctgg
     7501 ttcggcgccg aagcccccct ggcccggcat ctgccaggca tcatcgtcgg tcacgcggca
     7561 ctggccgaag ggctcggagt ccgggtcgag cctgcccttt gcgacctcgc ggcaagccgc
     7621 tactggctgg ccgagcgtga ggaaccggtc gctggccggg aatcgtcttg agcgattcgg
     7681 gggagatcgc agaaggggag gagctggcgg tcggcgcacc cgatccggct gatcaggccc
     7741 cggcgttgca cagccgcgag aacgtcaggt cggtccgggt caaggcgccg acccgcggca
     7801 accgcaagct ggagaagttc ctcgacgccg tcaaccggga tgacgaggtc aaggcctggt
     7861 ggtacatggc ccagacccat gccgagcggc tcggcatgtc cgaccactcc tgggtccaca
     7921 tgcagatagt gctcaacatc gccctgcgcc tgacccggct gctcgacaag ggcggggtgc
     7981 atggagcgat ggtcaccgac cacgggatga gcgcccgtga caccgaagtg gtggtggcgg
     8041 gcggagccct gctccatgac gtcggcatgt cgatccaccg ggccgaccac gaggaataca
     8101 gcctttttct ggctcgcgag gcactgccgc ggctgctcga cgggatctat gaggaacccg
     8161 agaagtcgat cgtcatggcc gaggtgcttc acgcgatcat cggtcaccgg cggcgcgggc
     8221 agccatacac ggtggaagcc ggaatcgtcc gggtggccga tgcgctcgac atggccgagg
     8281 gccggacccg ggtgccgctc gaatccggcc aggaagggat tcactcgatt tccgccgcgg
     8341 cgatcgaccg ggtcaacatt tcggccgggg agaaccgccc ggtccggctc gagatcgaac
     8401 tcaacaactc ggccgggata ttccaggtcg acgacctgct cgcgaccaag attcgcgaca
     8461 cgcccctggc cggccacatc gaggtcgtgg ccgaagtgaa gggggaaagc gagaagcggc
     8521 tcctttccgg tttccggctc ggcgcatgag caccagtctg gtcattctgc gcgagcaggc
     8581 cgggcatgaa accgggagcc atccggaaaa ctcacgccgt ttcccggggg ttctgaaccg
     8641 tctcggcgaa gacagccagc ggcacggcct gccgatcctc gaatcccggg aagccacggt
     8701 cgaggacctg ctgagggccc acaccgagtc tcatgtggag aacgtgcggg tcgcggccga
     8761 gaacggtccg acctggctgg acggtgacac ggtcgcgtcg cccctgagct acgaggtggc
     8821 ccggcgttcg gccggagccg cgctggtcgc cgtggatgcc gtgaacggca aggcggccga
     8881 agggaccgcg accaccagtt ccttcgcgat catccgtccg cccggccatc acgcaaccgc
     8941 cgaccgggcg atgggcttct gtctcttcag caacgccgcg atcgccgccc gctatgcccg
     9001 cgaggccctc ggaattggcc gggtcgcggt gctcgattgg gacgtccacc acggcaacgg
     9061 cacccaggac atcctctacg acgacccggg ggtcctcttc atctccttcc accagtggcc
     9121 gctttatccc ggcagcggct ggctggacga gaccggggtc ggggcggggc ggggcttcac
     9181 ggtcaacctg ccgatgccgc ccggttgcgg cgaccgggaa cacatcgagg gtttccaggc
     9241 gatcgccggg ccgatcctcg accagttcga cccgggtcta ctgatcgttt cggccggtca
     9301 ggacggtcat gtcgccgacc agctggccag ccagaacctg accgcggccg gttaccacgc
     9361 cctggccagt gcctccgctg attttgcttc gcgcaagggg atcgggttgg tcgcccttca
     9421 cgaaggcggc tacaacccga acacgctgcc cctgctggac cacgcggtct acgccggcct
     9481 cggcggattc accccggacc tgaccggaga caccccggcc cctgaactgg aagaaaccgg
     9541 ctggccggaa cgcctggccc aaatcagacg cgcccaatca gtgttctggg ccgtctgaag
     9601 cacccaaaac cgagcccctc ccaaccatca attgcttcag tgggtgggcg ggtttcacag
     9661 ggagggcggg tcggaccccg cccgacttgc gattaagccc ggtccctagg aattgaaccg
     9721 gtcaagatcg tgccgtcgct gatccttctt cgcgtccagc cggccctgaa tgagcgagag
     9781 gacggtgacc aggatcgcga agaaggcgat cagtccgaag cagaagaagg tgatgtaacg
     9841 gtcatcgacc cgaccggcgg ttccaacgcc gtcggcggct gccgcgtagg cgggcagggc
     9901 cagaacgaaa agcgcggtca gaagggcaac gagactgtta cggacacgat tcacgccccg
     9961 aaacctaccg caaaccgcag gacgcctgtg gctacccgac tgctccggcg cgggtgtgcc
    10021 tgttgctcct ggcccgggag ttgtgccttt gcggcacctc gggatccgag ggcaggtgag
    10081 aatggggccc ggcctggaag atcgaggagg gagttctcgg catatggcgc acccaactcc
    10141 gacttcgatc cggaccaccg ggccgagaac ggagttgggt gcgctatgtg gcgcaaattg
    10201 gtacctcgct gttttcggga ctcccggctc gcttcaggtc cagagccgaa tcttttcggg
    10261 gttgtgaatg agttgtgaaa atcggcttga tttcgcggct ttgcgtccgg ctacgcccta
    10321 cacttttcgg tagatgatcg gaagctcaaa atgaacgccg gccgggctcc aatgcgtgtt
    10381 gcggtgatcg acaccgattc cggcttcatc cgggtccttt cgaaccggat ggacgcggcc
    10441 ggctggcagt accgggtcct cgcctcgggc gtgccgccgg aggaactggt cgcgatgaag
    10501 atcaacgcgg tcgtgttcga tccggacgtg atcggcgaag gcggctggga gtacctcgag
    10561 cggatctgcg gcatgctgcc cgacctcggc gtgatcgtct gcgccaacgg gggcaccgtt
    10621 tcacagcggg tccgcgggct caggatcggg gccgacgact gggtcaacaa gccctgccac
    10681 ccggaagagg tgatcgccag gatcgaagcg gtggtgcgcc gtcgccgtcg tcattccaac
    10741 gaacaggacg ccggtccgct ggtcgtcggg gagatcgaga tccgccccga ccagttccag
    10801 gccttcatcg ccggccagag cctcgacctg acccgccgcg agttcgagct gattcaactc
    10861 ctcgcacaca atgagggcaa ggtcctcgac cgcgaatcga tctaccagcg tgtctggggc
    10921 tacgcgatgg cccacggcga ccgctcggtt gacgtcttca tccgcaagct ccgcaccaag
    10981 ctggagaggc ggtccccggc ctacacctac atccacaccc acttcggcgt cggctacaga
    11041 tttgcggccg aatcgtcgat ttcggccgcc gaggcgaccg ccaaggtcag ctccgaccgg
    11101 gccaccgatg atgcgggcgc tgcggcgccc ggcgtgccgg tctagcgctg caccttctct
    11161 cactcacgaa aacgggcacc cgatcaaggg tgaacccgct tcacaggagt tcacaactct
    11221 tttacagcag gtctacccgc aggtaacaaa ctggggcgga agcggtggaa cgcttcgaac
    11281 caccaggccg cccccgaaag gcgggaacca agtcccggag ggtgtgagtg accgagaaga
    11341 tccgaattca gtatcaggaa gaacttgacc acctcgagca gcaggctctc ggggccttcg
    11401 atctcgtgga ccgggcgcta ggccgcaccc tcgaagcgat cgaaaaccag gacgtcgagc
    11461 tggccgaact ggttgctgcc gacgacgacc ggatcgacgg gcgctacctc gagattcacc
    11521 agggtctgat caccctgctg gccacccagt ccccggtggc caccgacctt cggctgattt
    11581 ccgctctgct ccacgtgatc aagaacgcgg agcggatggg ggatcagtgc gtgaacatct
    11641 gcaagctgat cccgatcgtc ggccacgagc cgccttcgga cgagagcatg atccggttga
    11701 tcctcgagat gggggtccag gccagcagcc agatcattca ggccaggcag gccttcgccg
    11761 atcgcaacga gacggccgcc caggacctgg tccggcagga cgacctgatc gaccggctga
    11821 accgcgactg cttcaccctg gcgatcgaaa tcggcgacga gcccgaccgc cgtgaatggg
    11881 cgatgacgat gttgctggcc gcgcgtgcga tcgagcggat cggcgacaac gcggttgatg
    11941 tcggggaaca ggttgccttt gtggtgaccg gcctgttccg ggaattcgag gacgcgtccc
    12001 acccgatctg agccgggccg agcgtggaag cagcaaagtc ccagacgggc ggttccgccg
    12061 gccacgcgct gagggccggg cccctggaag tccggctcga tcagcggacc gtcctcgccg
    12121 acggcagggg aatcatcctc acggtccggg agttccacct gctcgcggtc ctggccagcc
    12181 ggccggaaca ggtcgtgagc agggaagagg tctacgacct ggtctggggc ggcgcgatgc
    12241 ccgaaaacga ccggtcggtt gacgtctacg tctcgaaact gcgccacaag cttgaaaccg
    12301 ccttgcctca ctggagcttc atccagaccc acatcggctt tggctactgc ttttcgccgc
    12361 gaccgctgaa cccctgactc gcggcaagtg acgggctttt cacctctttt cacaactttc
    12421 acaaggaagt aacaccgcgc gaaatctcca tctattgact gaagttcatt actgcgcaac
    12481 ccctcgaaag aaatccaaac tggaggaaac cttgaacttg aagaagtggc taatcgcgat
    12541 ccccctcacc ctggccctgg cgctgggtgt ggccgcctgc ggaagcagcg acagcgacag
    12601 tggcagcagc gggtccagcg gcgacgcggt ctccggcgag atcgccggcg ccggcgccag
    12661 ctcacaggaa gccgccatgg ccgcctgggt cgccggcttc caggagaaca atcctgacgc
    12721 caacatcgcc tacgacccgg tcggttccgg cggcggccgc gagcagttca tcgccggtgg
    12781 caccgacttc ggcggcaccg acaccccgat ggacgagacc gaactggccg atgtcgccaa
    12841 gcgctgcaag ccgggcgagt acatcgagat cccggtctac atctcgccga tcgcggttcc
    12901 gtacaacgtc gagggggtcg atgacctgaa cctctcggcc agcaccctgg cccggatctt
    12961 cgctggtgac atcaccaagt ggaacgatca ggcgatcgcg gcggagaacc cggacgcgag
    13021 tctgcctgac caggacatcg tggtcgtcca tcgctcggat gagtcgggca cgaccgagaa
    13081 cttcaccgac tacctgcacc aggcggctcc gaaggactgg accgacgagc ccgacggggt
    13141 ctggccgctc cagggcggcg aggctgccaa cggcacttcc ggcgtgatca gcgccgtcaa
    13201 gtccggcagc ggcacgatcg gttacgccga tctgagccag gtcggtgacc tgggcaccgt
    13261 caacgtcaag gtcggcagcg acttcgtggc tccctcaccc gaagcagctg tgaagatctt
    13321 cgacgagtcg aagcggatcg gtggcgaagg caagtacatc ttcgcgtacg agctcaaccg
    13381 cacctcgacc gcccagggtc tctacccgat ctcgctggtg tcatacgaga tggcctgcac
    13441 cacctacgac tcggctgaca cggccaagct cgtcaaggcc ttctacagct acatcatcag
    13501 cgacgaaggc cagcaggctg cggcctcgaa cgccgggtcg gctccgatcc cggcatccct
    13561 cagcaagcag atcactcccg ccgtggaggc gatccagacc agctgagctg aagcaagccg
    13621 gcagcgagaa ccccgcggcg gaggcacagg ccgccgcggg gtgatcgcag tcaggctcac
    13681 cagccgtttt tcgggcagga gatttcattg aacgcgggca ccgtctcaaa cagcgaaaca
    13741 ccaccttcag gcagggctca ccgccggttc ggtgaccgtg ccttttccgg tcttgccact
    13801 gcggccggcc tggcgatcct ggtcgtgctg gccggggtcg cggcctttct ggtcagcgag
    13861 gcctggcccg cgatcaccgc tccggtcgcc gaccttcccg acggaaagtc cctcctccac
    13921 tacatctggc cactcgtctt cggcaccgtt ctttcctcgc tcatcgcgat cctgatcgcg
    13981 atccccatgg cgatcgcggt ggcgctcttc accagtcacc tcgccccgaa gccgatcgcg
    14041 atgtcggtct cctacctgac cgacctgctg gccgcgatcc ccagcatcat ctacggcatc
    14101 tgggggatcg ccgtgctcgg gccggcgtcg gtgccggtca cccagtggct ttccgaccac
    14161 ttcggcttca ttcccttctt cgacggcccc gcctcgacca ccggacgcac catgctggtc
    14221 gccggggtgg tgctggccct gatgatcctg ccgatcatca gtgcggtcgc ccgcgaggtc
    14281 ttccagcaga ccccgaaagg gctccaggaa agcgccctcg ccctcggcgc gacccgctgg
    14341 gagatgatcc gcatggtcgt gctgcccttc ggccgctcgg ccgtggtcgc cggctcgatg
    14401 ctcggtctcg gccgggcgct cggcgagacg atggccgtga cgatcatcct ctcggtctcc
    14461 ggggtgatca ccttcgacct gatcagctcc gagaaccctt cgacgatcgc cgccaacatc
    14521 gcgctccagt tcccggagtc atccgggatc gacgtcaacg tcctgatcgc caccggcctc
    14581 gtgctattcg cgatcaccct ggcggtcaac atggcggctc gggccgtgat cggcagaagg
    14641 gaggccgccg gtggccggta gtgccgtatc gatgcccctg ggtgaaggct ccggggtcct
    14701 gccccgccgg gccggtttgc tcgtcttcgg gatctcggcc gtcgcctcgg cgctgctctg
    14761 cctggttctc ggcttcagcc cgttcgtctg gctgatcttc accgggctct tcagcctggt
    14821 cacgatcacc gtctggtcgc tggcggtcga aggccgtcgc cgggcactcg accgggtcat
    14881 gaccgtggtc atcgtcgaga tcttcgtgct cgccctggtg ccgctgattt cactcgttta
    14941 cacggtggtc agcgaagggc tggcccgctt cgacgtccag tttctgaccg aggacatgcg
    15001 gggtgtggtc ggtgaaggcg gcggcgcctt tcacgccgcg atgggcacct tcatcatcac
    15061 tgccttcgcc accgtcgcgg ccgtgccggt tggcgtgctg gccgcgatct acctggtcga
    15121 gtacggcaag gggcgcctca agcgttcaat cacctttttc gtcgacgtga tgatgggcat
    15181 cccctcgatc gtcgccggcc tcttcgcctt cgccctgttc gcgctgttct tcggccccgg
    15241 catccggctc ggaatcatgg gctcggtcgc cctgatggtg ctgatgatcc cgatcgtgat
    15301 ccgctcggcg gaagagatgc tgcgggtcgt tccgaactcg ctgcgggaag cttcctacgc
    15361 cctcggcgtg cccaagtggc tgaccgtcct gcgggtcgtg ctgccgaccg ccctggccgg
    15421 gatcgtcacc ggcgtcatgc tggcggtggc ccgcgtgatc ggcgagaccg caccgctgct
    15481 ggtcaccacc ggatcgatcg acacgacgaa ccttgacccg ttcgacggcc ggatggcgaa
    15541 cctgccggtc tatgtgtacg agcagtacaa gtttcccggc gttccgcccg aggccttcat
    15601 cgaaagggcc tgggccgggg cgctcctttt gatcctgatc gtgatggtgc tgttccttgc
    15661 ggcccggctc atcagtcgac gctatggagt ggagatccgc cgatgaacca gaccgagact
    15721 atgaaggaga aagagatggt tgacgcccag gccggaccgg ccgaccccat catggacccc
    15781 ccggccagcc cgagtgccac cgcgaccgtg gacgccctgg cgaaacggat cgacgccgag
    15841 gacctgaaca tctactacgg gagcttcctc gcggtaaagg actgcacggt cagcatccac
    15901 ccgaacaagg tgaccgcgct gatcggctct tctggttgcg gcaaatcgac cttcctcagg
    15961 tcgctgaacc gcatgcatga gctgattgac ggcgcccggg tcgaaggccg ggtcctgctc
    16021 gacgggatgg acatctactc gccgtcgatg gatccggttc tggtccgcca gcagatcggc
    16081 atggtcttcc agcagccgaa cccgtttccg accatgtcga tctacgagaa cacggcggcg
    16141 ggcctgagac tgaaccgccg caggctgaag aagaccgaga tcgacgggat cgtcgaacgc
    16201 tcgcttcgcg gcgcccacct ctgggacgaa gtcaaggacc ggctcgaaaa gccggccagc
    16261 gggctctccg gcggccagca gcagcgcctc tgcatcgccc gcgcgacggc cattgaaccg
    16321 gaagtcctgc tcatggacga gccctgttcg gccctggatc cgatcgctac cctggcgatc
    16381 gaggacctga tcaacgaact gaagaagcgc tacacgatcg tgatcgtcac ccacaacatg
    16441 cagcaggcgg cccgggccag cgattacacc gggttcttca acatcgagaa aagcggtgac
    16501 ccgggcgtcc tggtcgagat cgacgaaacc gagaagatct tctcgaaccc gtcgttgcag
    16561 gagaccgagg attacgtcag tggccgtttc ggttgagctt ccggtcccgc cggagggccg
    16621 aaggctccga accggcccgt ccgcttcgcc tgccagaatc gtttgggtaa agacgatgct
    16681 ctgcgcagcg atcgacatag gttcgaacac cacccggatc ctggtcgccg agcccgatga
    16741 aggcaagctc aagaaggtga tggagcagcg ggcctacacc aggatcggcc aggcccggct
    16801 ggatgacggc acgattccgg ccgaaaaggt cgccgaagtc gccgaagtgg tcgacaccca
    16861 ggtgaaactt gcgcgcgaac tcggcgccgg gactttccgg gcggtcgcca cggcggcact
    16921 gagggacgct ccgaacggga aggaagccgc ggccgaaatc gaggcggtgg cgggcattcc
    16981 ggttcacgtg gtcagcgaga acgaggaagg ccggctcgcc ttcctcgggg cgaccaaggc
    17041 gctcggctat caggtcgaag gccgggtggc cgtggtcgac gtcggtggcg gttcgaccga
    17101 gatcatcatc ggcacggccg acggcggggt ggaacaggtc cgctcctggc cgatcggctc
    17161 gggccagctg gccgaggaat tgatcagctc ggacccgccc tcggcttccg agatccgccg
    17221 ggtgcgggac cgggtcgacg aagtcttcgc cgaggtccag atcacccagc ccgagcaggc
    17281 cgttgcggtt ggcggttcgg ccacttcact gcgcaagatc gtcggcgccc ggctcgaata
    17341 cgagactctg gaacgcagca tccgggtcct cagcggtgac gaagcgctcg aagtcggccg
    17401 ccagttcgaa ctcgatccga ttcgcgtccg catgctggcg accggggtcc tgattctcga
    17461 gaaggtctcc gaactgctcg gccaggacct gcagatcggc aagggcggga tccgcgaagg
    17521 gatcgtgctc gacatgctga acggcggaat cgacgtttca gaacccgcat tgacacccgg
    17581 ctgagtcggc ctgaggtcta gggttccggt tgatgagcac cgaatccgat ccccgcttcc
    17641 gccttgccgc gtcgcaggtg ctcgaagagc gggcgaccca gatctcggtc cttgccaagg
    17701 gtgtgctcga cgtgaccgag accggagcgg ccgacgaact ctggctcgct tcccgtcggc
    17761 ttcgcgccgc acttgaggtc tttcgctcct gtttcggccg ggcccagtat cgcgagggaa
    17821 gggcggaagc caggcggatt tcgcgggccg ccggggcgag gcgcgacatc gacgtggtga
    17881 tcgccgccta cgaaagcgtc ggtgaggaaa ccgccccggc cgaatcggag gggatcgacc
    17941 gtgtgatctc gtcgctgcgc cgtgaacagg ccagcgccaa ccgcgccctg gcccaggtgg
    18001 tccacggccg ccgcctgcag gcgttccggg tcaggatcga ggaaatcgcc gattcggcgg
    18061 tcgaagcaga cgccgccgac cgcgcccatg actaccggcc ggttgaaacc ctgactcccg
    18121 aagccatcgc cctgatctcc aaacggctgg cccggctgcg caaggaagcg gttgacagcc
    18181 tcgatccgga aggcgtcgac gcgcaacacc ggctgcgggt cgcggcagag cggctcaggt
    18241 actcgctgga gctgaccggt gacgccctcg gatcccaggc tcacaccgcc cgccgcgcgg
    18301 cccgggggct gcaggaggtg ctcggcgaga tgcgtgactg tgacctggcg gtgccgccgg
    18361 cccggcaggc gatcaccgac cttgaggccg aagacgtgaa gacgatcatc gatcgcgccc
    18421 gcggcagccg ggacctcgat ccgatcctgg tccaggccgc accgaaccgg gcggcctacc
    18481 gcggcctcga actcgccgtc gttcacatga cggcccggcg gcagatgctc tatgaacgct
    18541 tcaaacgact ctggcacgag cagtcccgcc agggcgtctg ggtcgctctt gaaacctcac
    18601 tgaaagtaga atcctgacct tggaaacgac tcccgatccc tcctggttct tcaatcgcga
    18661 actctcctgg cttgatttca accagcgggt tcttgagctg gccgaggacg attcggcgcc
    18721 gctcctggaa cgggtcaagt tctgcgcgat ctttgcctcg aacctcgacg aattcttcat
    18781 ggtccgggtg gccgggctgt gggatcaggt cgatgccgga atcgacgccc ggggcccgga
    18841 cggcctctcg ccgcgggaac agatccaggc gatccgcagc cggtcgctgg aactcgaccg
    18901 ccggctgacc agggtctttg aacgcaagtt gaagccgcag ctgaccgaga acggcatcaa
    18961 ggtcgcgacc ctggccgacc tgaacgggac cgagcgggcg gagctggacc ggattttcga
    19021 ccagcaggtc ttcccggcgc tgaccccgct ggtgatcggt ctcggccgtc ccttccctta
    19081 catctcgaac ctctccctga gcatcgccgt cctgcttcgg gaccgcgaga cctcgacccg
    19141 cgtgctcgcc cggatcaagg tgccgaagga gctgctcggc cgcttcatcg aactgagcag
    19201 cgagaaggag cgggtgctgg tgccgctgga agaggtgatc gcggccaatc tcgatcagct
    19261 gttccccggt gttgaagtgc tcgatcactc ctatttccgg gttacccggg acgctgattt
    19321 cacggtgtcc gacgaagccg acgacctgct cgaagcggtc caggaggaag tccgtcgccg
    19381 gcgtttcggg gaggtcgtgc ggctggaagt cgccgacggg atgaacccgg agctcaagga
    19441 gctgctggtc gagctgcttc gtcttcagca aaacgaagta ttcgaagccg gcggcctgct
    19501 cgacctgacc gacctctggg acgtggtcaa gctgggcggc ttccaggagc tgcgcgacaa
    19561 gccctgggct ccggtcaccc agccccggct gcagggcgag gacggagacc cgcccgacat
    19621 cttcgcggcg atccgccagc gcgacgtgct ggtccatcac ccctacgatt ccttctcgag
    19681 ttcggtcgag cgtttcatcg cccaggcggt gagcgatccg gacgtgctgg ctatcaagca
    19741 gaccgtttac cggaccagcg aggactcgcc gctggtctcg gccctgatca aggcatcgga
    19801 aaggggcaag caggtcgtct gcatgctgga actgaaggcg cgcttcgacg aggaagcgaa
    19861 catcgagtgg gccaaacgcc ttgaagaagt cggggttcac gtggtctacg gcatccccgg
    19921 gctgaagact cacatcaagg ccctgctggt tgtgcggcgg gaaggcgacc aggtccgcaa
    19981 ctacgtccac atcggcaccg gcaactatca ctcgaccacc gcccggctct acacggacct
    20041 cggcctgttc accgccgacc cggagatcgg cgccgacgtg gccgagatgt tcaacctgct
    20101 gaccggttac tcgcgcccgg ccgactaccg ccgggtgctg gtctcgccgc attcgatgcg
    20161 ttcctcgatc ctggccgaga tcgaagccac ggtcgccgcc cacaaggcag gccgggaggc
    20221 gaagatcctg atgaagatga actcgttggt cgaccggacc tgcatcgagg cgctttaccg
    20281 ggcctcgcag gccggtgtgg aagtcgagat caacgtgcgc gggatctgct gcctcaggcc
    20341 cggggtgccg gagctttcgg agaacatccg ggtggtctcc ctggtcggcc ggttcctcga
    20401 gcattcacgg gtctatgcct tccagcgcgg aggggactgg aacatcttca tcggctcggc
    20461 cgacctgatg ccgcgaaatc tcgacgcgcg ggtcgagctt gtgaccccgg tcgaagacga
    20521 gatcgcccgg gatcagattc tcgacgtgat ggaacgctgc ttctccgaca actcgaactc
    20581 gtgggtgctg gacagttccg gcaactggtc ccgcctcagc cccgagggcg gcgagcgccg
    20641 caacgcccag gaggaactga tggaacggca ccagaccctg gccgcaaccg cgcttccgca
    20701 ggccaacgga tgaaaagacc atgaaaaggc cgtgtaaagg atttgcggcc cgctctggta
    20761 ggttggcccg gctctgatgc gtttctcgct actgcccagg gacaacacct tcttcgacct
    20821 cttcaacgag gccgggcaga acaccctgcg cgcagccacg aagctgaacg acatgatggc
    20881 cgcatggcct gatgacaacg gcctcggccg cgagattctg atcatcgagc aggagggtga
    20941 ccggatcact cacgacatca tcaaccggct caattcgacc ttcgtcacgc cgattgaccg
    21001 cgaggacatt tacggcctcg cgaccaagct cgacgacgtg gtcgattaca tcgaggaagc
    21061 ggcggatttc ctggcgctgt accagatcga agcgccgatg gaccaggccc aggacctgac
    21121 ccggattctg gtcggctgct gcgagcagct ttcgctcggc ctcgagaacc tgccgtcatt
    21181 cagggatctc gaccagtact ggatcgagat tcaccggctc gaaaacgacg gcgaccgggt
    21241 ttcgcgtgac gccgtggcat cgctgttttc gaacgggatc gatccgatgg tcgtgattcg
    21301 ctggaaggac atcttcgccg tcctcgagaa ggcgatcgat gcaaccgaaa cctccgccca
    21361 catccttgaa ggcatcgtga tcaagaacag ctgacgacgt ggacctccat ctgatcctcg
    21421 tcatcgttgt cgtctcggca ctggccttcg atttcaccaa cggctttcac gacaccgcaa
    21481 acgtcgtggc cacctcgatc tcaaccgggg cggcttcacc caaggcggcg atcgccttcg
    21541 ccgcgatcct caacttcatc ggcgccttca tctcgatcga ggtggcggcg acgatcgcct
    21601 cggacgtggt cgaccagtcg gcgatccagg gtgcgggctc gctcaacgtg gtcttcgccg
    21661 gtctggtcgg cgcgatcgcc tggaatctga tcacctggta cttcggcctt ccttcctcca
    21721 gttctcacgc cctgatcggc ggcatagtcg gagcggccct cgccgactcc ggcagccagg
    21781 cgatcgagac cgccgggctg gtcggcaagg tgctggtgcc ggccatcgtt gcgccgatcc
    21841 tcgccttcgt cgtcgccgcc ctggcgatcg gcctgatcta ccggataacg gcccggcttc
    21901 ggcccggtcc ggttacccgt ggataccggc tgggacagat tgcctcgggt ggcctgctcg
    21961 cgctcgcgca cggaaccaat gacgcccaga agacgatggg tgtgatcacc ctcgcgctga
    22021 tcgccgacgg ggcgctgccg gaaggcgctt caccgccgac ctgggtgatc gtcgcttcgg
    22081 ccaccgcgat tgcgctcggc acctacagcg gcggctggcg gatcatcaag acgaccggca
    22141 tgcgcatcat caagatggac gccgcccagg gcttctccgc ccagggcgcg ggtgccgcgg
    22201 tgatcatggc ttcatcgcat ttcggattcc cgctttcaac cactcacgtg atcaacggcg
    22261 gcgtgatggg ctccggtgtc gcgaagcggg tctcggcggt gcgctggggc gtggcgggca
    22321 acatcgtcac tgcctggatc ctgaccctgc ccgccgccgc cgcgatcggc gcgctctgct
    22381 tcgggataac cgagatcttc ggcgacggtg ctgccggtcc ggtcatcatc agtgcgatcg
    22441 ccgtcgtcct gatcgtgatc gccttcgtgc gacgctcgcg ccagaccgag gtggttgacg
    22501 tgcagaccga ggtggcctga gccatggacc tgttcgcctc ggtcgtcgcc tggaaggaac
    22561 tcggggacac ggtgctctac gccttcgtcg gagcgatggc ggtctcgctg gccgcgtccc
    22621 tggggatctg gggggcgacc aagtacgtcg acttcaatca ggacgggcgc ggggtggttg
    22681 cgggtatggc gctggcggtc ggggccttcg gcctggtggc gacgatcggg atcatcgccc
    22741 tcggcatcta cctgatggtc tccaagtaga aggaagttcc ggcagcccgc ggtcatttag
    22801 gcttgtcggg tgactatcgc caggaacatc gcgatcatcg ccctgctcgc ggccggagtt
    22861 gccttccttc ccaacggggg caacgttgcc gatgcggtgc tgaccacgat caccatggcc
    22921 ttcctggccg gtctcgcctg gacggtctac cgactcacct atgagttccg ttcggggctg
    22981 ctggcgcttg aaggttcgcg ccgggtgatc ctctattcct gtttcggcct gatcgtcctg
    23041 atgatcgccg gtgcggacaa gatgttttcc accggcttcg gcacgatgac ctggctgctg
    23101 ctgatggcct cgggcctggt cggcgtctgg ctgatcgtct cggaggctcg cagctactga
    23161 atttccgggt actcagctgg aatctcttcc acggccgcga cttcccgccg gcgcctggca
    23221 aggaagatcc cggagttccg ccgggcgacc tcctctggcg gatcacgggg cgaccgatcc
    23281 cgggcaagaa gcatgttcgc ctgaaccggg acctctttca cgagtttgcc gccctgctcg
    23341 cggccgcgaa atgggacatc gccctgctgc aggaagttcc gcctcgctgg gcccaccgcc
    23401 tggccgaagc gaccggggcc gaggcccgga ccgcactcac ttcccgcaac tggttgcggc
    23461 cggtcatgtc accggtcgcc aggttccggc cccaactggc cgggagctgg gaaggagggt
    23521 gcaacctgat cctggtccgg ggcgcgaccc cgggcctgga taaatcccgg cccggggccc
    23581 acatcaggga gttccggaag tcgaccctga cctggtttcc tgagcgacgg gtcatgtcga
    23641 tggtcgaact cggctgcggc ctctgtgtgg caaaccttca cgccagcacc ggcgaaaggg
    23701 cggcggggga cgtgatgcgg gcggcgcggc tggccagcgg cagggcgggg gatcgccccc
    23761 tggtcctggg tggcgatttc aacgccaggc cccgttcgag cgatgttttc gaccggctcg
    23821 aaggcgagtt cgggctggcc ggaaccactg cgccgggttc gatcgaccac ctgctttccc
    23881 gcggggtgga agtgcttgag cagccggaag cctggccgcc ggcgcggcgc gacgtgcccg
    23941 atccggagac cggcctgatg gtcaggctct ccgatcacgc accggtgctc gcccggattc
    24001 gggtctgagc accccgacgt tgcaaatcgt tgcagttgta gagccacatg tgagcttttt
    24061 cctgccacgg tccgggcatg gatgcggatc gcgaaaaggc aggctcgggc gggatcggtt
    24121 cgcggcgggg ctcggtctcg ctcgagttga tcggttccct gccgatcatc ctgctggccg
    24181 tgctggtcgc ggcccagatc gcgatcgcgg gggcggcact ctggtcggcg ggggtggccg
    24241 cccgggccgg ggcccgggcg gtgctcaccg gcaactcccc gcgcggggcg gctgaacacg
    24301 cgcttccatc ggtcctgcag gggggcctga aggtctcgca ggaagacggg gtcaaagtcg
    24361 gggtgagggt gcccgggctg ctgcccgggt tcccggagac ccatgtctac ggttcgagtt
    24421 cgctgggaga tggctgatga attccgggcg ctcgggccag gcgacggtcg aggcggtctg
    24481 cggcatcgcc gccctgatgc tggccggcct gctctgcttc cagctgcttg ccaccggtta
    24541 caccgcgacc atcgccgacg gggcggccga ggctggcgcg gtcgcgctga tccggggcga
    24601 gccggtcgag ccggccgtgc tagaggcatt gcccggctgg gcccgggacc gggtctcggt
    24661 gtcgagggtc gggtcccggg taaaggtccg gctccggccg ccttcgctct tctcctcgat
    24721 gtccgaaaag cttgcggttt cggccgaggc gggcgggagg ccgggatgag cgagcatctg
    24781 ctgatcgggt cgatcggcga ttcgcgtggc gccgaacggc tcgccgccga acgggttctg
    24841 gaatcggcgg ccgggcgggt cggcgtggtg ctggagaccg gtcgggagcg gccccggccg
    24901 gccagcctgc tggctcccgg cgaggccaag gagctggaat cggaactgct ggaggccctg
    24961 ccggagcttg agccgaaagc ccgcgggagc ctcgtctacc tgtcagccgc ccagtcagag
    25021 cggctggccg gggaagtgat cagcttgcgg gagatcgtgg atgaacggcc cctggccgtg
    25081 attgcggggg ccggccggta ccgcgagctg ctggaactcg gctgggaccg ggtggtgatc
    25141 agatccgacg aggccggaca ggatgccccc gaggtcgatg cgccggtttc cggagatgcc
    25201 gtcgccggcc ccggcgccct gaccgggctg gtcgtcgaag aggcaggcga gttcgccgat
    25261 cgggtcgaac tcgtgcgggg cgcggccggg aaactgtggc ccgccggcgg gcggctcctg
    25321 ggccggctcc gttcgaccgg cgggcaggcg accccgctgg tgcttggatg cgtcttcgca
    25381 ctggtgctgg cgaccgtggc ggtggtcgcg atttccgggg cggtgaccgg caaggcaagg
    25441 gctcagcggg cagccgatct ctcggccctt tccgcagtcc gttcgatgaa ggatgacctg
    25501 ccgcggctgc ttgctccgcc gcgcctgccg aacggactgc cgaacccggc ccacatgccg
    25561 aagcaggtct acctcgcgcg ggcgcggctg gcggcgatcg gggcggcgac atcgaacggg
    25621 gcttcgccgc tcgcggtttc ggtccgtttt cccgactccg cctcctatgc cccggtgcgg
    25681 gcccgggttt cgatcgccac ctcggtccgg cccggaaccg gtggcggggc acaccgggca
    25741 ccgccgaact gggccgaggc ccgggtcagc gttccggtct cgatggggtc ggtgccggcg
    25801 atggccagcg gcggcggcta ctccgggccg ctggccgaac ggcagggtca cggcatgcgg
    25861 ccggacgtgg ccgccgcctt tgacgcgatg gtcgcgtccg ccgcggcagc cggagtaagc
    25921 ctgaccatca attcggcctt tcgttcggac gcggaacagg cagccctctt tgcggccaac
    25981 ccggatccga cctgggtcgc gccgcccggc cagtcgcttc accgctgcgc gaccgaactc
    26041 gatctcggtg cctccgacgg ctacagctgg ctggcagcca acgccgggcg tttcggcttc
    26101 gtccagagat acagctggga agcctggcat tacgggttcg tcgccggccc ggcgccatgc
    26161 tcggatgcgg gaaactcggt cggcatggac gggggcgacc gttcggcggc gctctcgcgg
    26221 gcggtgccgt cattcacccc gggctgggtc cggcccctgg tccttcgcgc agccatgaag
    26281 tggaatgtct cggccgcatt gctggccgcg cagctcttca ccgaatcggg ctttgacccg
    26341 aacgccggca acgggatcgc cttcggcatc gcggccttca cgccggccgc ggcggccgag
    26401 tatgggctgg atgatccgtt tgacccggcg gcggcgatca tggcccaggc ccacctgatg
    26461 agcgacctgc tccggcagtt cggttcgccc gctctcgccc tggccgcata caacgccggt
    26521 ccgggcgcgg tcggttcctg cg
//
LOCUS       DAOQKN010000011        26419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_753_length_26419_cov_6.098126, whole genome shotgun sequence.
ACCESSION   DAOQKN010000011 DAOQKN010000000
VERSION     DAOQKN010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 26419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26419
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_753_length_26419_cov_6.098126"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..860
                     /gene="cysW"
                     /locus_tag="PLJ64_01565"
     CDS             48..860
                     /gene="cysW"
                     /locus_tag="PLJ64_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HNC92212.1"
                     /translation="MTASGRLSKYGLRFVALFYLTALLLLPIGVALVKTFEDGIGEVI
                     KQITEPDAIHALKMSLLTVIIAVPLNTVFGIAAAHVVVRTKFRFKWAINALIDMPFAV
                     SPVVIGLALVLVYGTGGWFGADLAAMGIQVIFSTPGIVMAVIFVSLPFVAREVIPVMQ
                     EIGTDQETAAETLGASPWQTFRKITLPAIRWGVTYGIVLTTARALGEFGAVTVVSGNI
                     SGETQTLPLYVSKQFETFNSVGAYSASLILAVLALGTLLSMNLLRRREDQDD"
     gene            853..1932
                     /locus_tag="PLJ64_01570"
     CDS             853..1932
                     /locus_tag="PLJ64_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HNC92213.1"
                     /translation="MTEITDHLNPESHGIEVREVNKEFGDFQALKDVNLIVPDGSMTA
                     LLGPSGSGKSTLLRTIAGLEVPTSGQVFIGGEDQARKPVRTRNVGFVFQHYAAFKHMT
                     VFDNIAFGLKVRKWDKQKTRDRVNELIDLVQLTGLAKRYPSQLSGGQRQRMALARALA
                     VEPSVLLLDEPFGALDAKVRKDLRQWLRRLHDEVHVTTIFVTHDQEEAMDIAEQIVVM
                     NNGQIEQTGSATDLYDRPENEFVMGFVGEVNSLGGRLVRPHDTEILPEPTEDTTEVEI
                     DRVVRLGFSGRVEMLTVPDKKEVWAQLTRSELETLSLEAGTRAYVRPRAEAVTKEDTG
                     ALEEALREESPATDTPIPAEPALPV"
     gene            complement(1871..2242)
                     /locus_tag="PLJ64_01575"
     CDS             complement(1871..2242)
                     /locus_tag="PLJ64_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC92214.1"
                     /translation="MTGSTDTWQTADGRTTRPALTEVCPHFHAGIELIGKRWSGAIIW
                     ALDDGPLRFAELKRSIPGLSDRLLSQRLRELEDAGLMTREVEDGPQVKVTYSLTEMGE
                     GLRPAVLALREWACRWQDSLP"
     gene            complement(2313..3455)
                     /locus_tag="PLJ64_01580"
     CDS             complement(2313..3455)
                     /locus_tag="PLJ64_01580"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF033740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarP family serine protease"
                     /protein_id="HNC92215.1"
                     /translation="MTALDWGIAAFTFLLALWGFRQGLIVGALGLAGLAVGAILGSRL
                     APVFLDHGSNSPYAPLAALVGAIFVGSITLALSVTLGERVRDSTVSHPFWEVIDGIGG
                     AVLIAAVGLGIVWVLAAVAVYYPSSDGLRRDVQKSLLVGAVNDVMPPSGPLMQALHRI
                     DPFPRIAASTGEIAKPDQGTGSKAAVQKAAESVVKVSGTCNGFGVMGSGWAVGPDTFV
                     TNAHVVAGQDDTTIQTNDGQSASATPIAFNTRNDIAILHADVDLPALPALARARRGTA
                     AAVIGYPNDGPLTITPARAGETRILIGQDSYGSGSFERSMLALRGNVRHGNSGGPLVD
                     SRGQVLGTVFAATTEGPQGGLAIPNRVLADISKKVSGKEVDTGPCA"
     gene            3537..5270
                     /locus_tag="PLJ64_01585"
     CDS             3537..5270
                     /locus_tag="PLJ64_01585"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNC92216.1"
                     /translation="MGRPPSRQERQKRLKTRALPLVGVALISFIVGMVKGCPGSPNRD
                     AAEQYVEYWAEGNYVAMYDMLSTKSREGITQERFIARYGEASAEATIESIEQSDAEGD
                     ETNAEIPMEVDTLAFGTIDQSLKVTFGSDGIAWQNSLLFPGLELNEELTRVTKLPGRA
                     KILAEDGQVMAEGPVDAREHPLGDSMIDVTGVTGPPDQEEGPDLTSLALGYEPGEDTG
                     ISGLELAYNARLTGTPGGTLFAKPIEGGTDGQGRELGSGTPAPAKPLKTTIDPDLQES
                     AVANLGGVSGGVTVLDAKKGFIKALAGSAYSVLQPPGSTMKMVTATAALETGAATMDS
                     SYDYVTEAPADGRMIQNAHGEVCGGTFTEAFAKSCNSVFAPLAEQIGPEQFTRTAELY
                     GFNRPPQVWNDEGLAAVNPPTPTLPEADDYDSDLGVSGIGQGRVQATPLLMASVAQAI
                     SNKGISLPTPITTEADLQPEMKPVRVTSQKVASQVADLMVAVVTSGTGIAAAIPEAQV
                     AGKTGTAEVGPSGEVDANGDDILIEDAWFAGFAPSDKPQLAICVNVLDASGDGGTVAA
                     PIAAAILSDGL"
     gene            complement(5850..6203)
                     /locus_tag="PLJ64_01590"
     CDS             complement(5850..6203)
                     /locus_tag="PLJ64_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92217.1"
                     /translation="MSDGPGHLRLADHGPALAGQIAAGLGAALALLGPFSDVIAGIGV
                     AILIIGVVLAAPAGRQPGPVMAEWWSVLALAALATLIGYGLAFWLPAPGGVLLTIGAV
                     TSLAAVFFGTPAQPE"
     gene            complement(6204..6761)
                     /locus_tag="PLJ64_01595"
     CDS             complement(6204..6761)
                     /locus_tag="PLJ64_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92218.1"
                     /translation="MERLSKSNLFRGLVLALVVAISGLALAACGASHEEKEGLAEGEI
                     VELGPLQYNVLFTRPLNRNDIEDSQYLVGKPAPGPDQMYIGVFVHVHNDSDDPQTIPD
                     SFQIETTAGRKYENIPSKSVYALQPGATVEPHDNIPQVDSTAQVGPIGASMLLFLIDR
                     ESAEQRPVELTIDGEDGPATFDLDL"
     gene            complement(6808..7317)
                     /locus_tag="PLJ64_01600"
     CDS             complement(6808..7317)
                     /locus_tag="PLJ64_01600"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNC92219.1"
                     /translation="MGPVFTEVDIDAPREEIFDYLLDFDTRPFLYGDSVENFRLLRLD
                     SRGIGAGARWQFKRKKAWADSAITATEAPRRISERGSTGSYNRNVTGTEWEMEEISTG
                     VTRVRITFWIVPAGFSRVHNRLTGGAGWHRRRLDKVGKRLREAIESARREKVEVPVAG
                     GNRHATGVA"
     gene            7377..>8080
                     /locus_tag="PLJ64_01605"
     CDS             7377..>8080
                     /locus_tag="PLJ64_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNC92220.1"
                     /translation="MSTTIAILSQKGGTGKTTLTRSLSEGLRRAGLSVLAIDADPQGN
                     LSEYFGIPADAFPTLGEVLAGRERAVDAIHTGPGGGEVIPANLGLAEAELMLAGKIGR
                     ELTLRNALREPKRRFDVILIDCPPTLGLLTVNALVASDKAILSTQAEHFSEQGAEQAM
                     EVIGLARENLNPELELLGLLLNIADMRTNHARSTLETMREQFGDLVFDTVIRRSIVYP
                     ESARAAKPIFDYRKRKS"
     assembly_gap    8081..8180
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8212..9087)
                     /gene="thrB"
                     /locus_tag="PLJ64_01610"
     CDS             complement(8212..9087)
                     /gene="thrB"
                     /locus_tag="PLJ64_01610"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HNC92221.1"
                     /translation="MTERHRLVRVPASSANLGPGYDVMAVALDLHLELEVKETGTFSV
                     SCDALDVPLDRSNLVVRAFETLQPADGISFRVGGEIPLARGMGSSAAAIVAGLVAADH
                     LFELGLERQDLLERATALEGHPDNVAAAICGGFVICTGGPRLTATVIEAPEGLEGILV
                     IPEVEVSTRKAREAIPEQIPIKDAVANTASAAHLALGLARGDSDLIGMGLHDRLHQER
                     RRHLFPKSMEIVEVAGEIGAIGATISGAGPTVLVWTNWQETGGVVEQLRERCEGWAEV
                     RRVGFSPLGADVPEL"
     gene            9119..9928
                     /locus_tag="PLJ64_01615"
     CDS             9119..9928
                     /locus_tag="PLJ64_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC92222.1"
                     /translation="MSSSSANPPDPSPPALEITDLVKRYPTGVEALKGVSLEIADGEF
                     FGLLGPNGAGKSTLIHCSTGLASPTSGEIRVFGHDAVGDYRQAREAVGLAPQELNIDW
                     FLTVEETLDFHGGYFGMGKKDRKERVDELLESFSLVDKRNDRTRDLSGGMKRRVILAR
                     ALMHRPRLLILDEPTAGVDVELRLELWHYVQQVNSEGTSILLTTHYLEEAEQLCSRIA
                     FINGGEIVDHGSSDELAERFGVGSLEDAYLELVGRKELSRAHVGIEAEDGQ"
     gene            9925..10692
                     /locus_tag="PLJ64_01620"
     CDS             9925..10692
                     /locus_tag="PLJ64_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC92223.1"
                     /translation="MSPSMVRLVSLTRREVSRFLKIPRQTVGAPLLETFLYISIFGAA
                     LGSRVGDLHGYEYIVFVVPGLIMMAFAINAFTNNSSSILQQKFQGAIDDQLSSPASPG
                     QLLLAFTLGGFLRGLLISVVTFLVASLLIDLPVKHFYILIPALFLVGWFFASLGVLVG
                     VRAEQFDDVSFAQTFILQPLIFLGGVFYSASLLPEPYETLTHFNPVYYMISLVRYGFL
                     GVADISVWLSLALLTAATAALTFANYRMFKSGYKLRR"
     gene            complement(10700..12712)
                     /locus_tag="PLJ64_01625"
     CDS             complement(10700..12712)
                     /locus_tag="PLJ64_01625"
                     /inference="COORDINATES: protein motif:HMM:NF040438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HNC92224.1"
                     /translation="MGSGRKFSAVVSTIAASFFMLVMAGPASADLSQIPTATVTGPIA
                     VTADSHPFLATDIDLDKYGYVENEYFLAGDAYRYDMTGAIDTPAAKITTGGSADDGKY
                     PFKTRIVVRRPANPADANGVVIAEWNNVTSTQDVEFNWFGDPYFLLKHGYTFVGVTAQ
                     NVGVASLKAFDSDRYGTLTVNGNDTVPTGTGLDADALSYDVYSSVVKALKGSRNGVDP
                     LGGITPSMVIASGESQSCGRLVTHYNRIEPIHEVVDAYLLTVCTSALRGDRGEKAIRI
                     ITETENRVPRTVASDPDTGSIRHWEVAAASHLPRVAFDNLNPVFSRDFATVTANCVKF
                     PLSRIKWPYTQNAAISDLVGWVGDDAAPPIAPRGEYQASPPDMANQLERDENGIALGA
                     IRYPDVTVPTATNDGINSAAPGGSIFSAFCGLFGSSVPFSPELLHSLYTDQADYLAQY
                     SEAADALADSGFILPEDADRLKGDSREYARLRPSLPSVIGGTSNKGGFQLVWVGTEAP
                     DTTFEVQRASTKKPNAWAKVKTKSVSDSTATIADAAQGTAYFRVNSATVLPGTNISEP
                     ETVVTPFSEASVAVKIDRTGPAKPKIVLKGHKVKGQFRGPVRVKVIGKPDGKLPDGTA
                     GVGLNPKSVPKTRKVTRKGKTVIKIRTRDKLGNQSPVAKVVIRIRR"
     gene            complement(12768..13802)
                     /gene="thrC"
                     /locus_tag="PLJ64_01630"
     CDS             complement(12768..13802)
                     /gene="thrC"
                     /locus_tag="PLJ64_01630"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNC92225.1"
                     /translation="MPQPLIERYRDALPLDPGDPVVSLNEGSTPLVEAPRLSERVGAR
                     VWLKVEGANPTGSFKDRGMTVAVSRAAGRGAKAVICASTGNTAASASAYAVRAGLRGA
                     VIVPEGKIAIGKLAQSLMHGARVVALEGNFDDALTIVRQLAERNPIELVNSVNPYRIE
                     GQKTAAFELIDELGQAPDALAIPVGNAGNVTAYWKGFQERGTAPICYGFQAAGSAPLV
                     DGAPVAHPETVASAIRIGNPARWEEAMNAFNSSRGRVAAVSDEQILDAYRWLAANEGV
                     FCEPASAASVAGILNHGLPTVEGMEKPETVVCVLTGHGLKDPDTALGKAPSVINCPNE
                     IGAVEEAVFG"
     gene            complement(13809..15035)
                     /locus_tag="PLJ64_01635"
     CDS             complement(13809..15035)
                     /locus_tag="PLJ64_01635"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNC92226.1"
                     /translation="MSENLRIGLLGRGTVGSAFDELVAERADQVEAATGRRPEVTGVL
                     TRSQGDFGEILPDADVIVELMGGTDTAREHVLAALRAGVPVVTANKQLLAQHGAELFA
                     AAEEGGVQLRFEAAVAAVVPVIRVVQESFGAVEFSKVFGIVNGTTNFILSEMAATGAS
                     YAEVLKRAQELGYAEADPTEDVNGADAAAKMAILARLAFHTPVTLDQVDFEGIEDIQP
                     DDLAYAKELGLSLKLLGVAEKLEQGITVRVFPCFLYPGHPLAPIEGPFNAVMVEAPEI
                     TEVTMSGPGAGGLQTAAAVLGDVVSVAAGDARVHTADRDLPVLADVTSSFYLHLEVAD
                     EPGVLAEVAKVLSDGGVSVKSVVQRGLGDDARLVMVIHRCLESEFKAAVAQIAELDSL
                     KSGPRFIRVIEEEFTG"
     gene            complement(15032..16285)
                     /gene="lysA"
                     /locus_tag="PLJ64_01640"
     CDS             complement(15032..16285)
                     /gene="lysA"
                     /locus_tag="PLJ64_01640"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNC92227.1"
                     /translation="MPYPVGTTTNDAGHLEIGGCDVIELAREFGTPSYLFATAEMRER
                     ARAYQRAFAAHTDHFEVLFASKALPVTAAYRLFAEEGLSVDVASGGELTMALTAGFDP
                     ARIYMHGNNKTDAELVLAGNSGVGHLIVDSFDEIDRCERVLKRKQDVLIRVTPGIKPS
                     THSFIQTGQLDSKFGFGLEDGTAAKAITRVLASDTLNLVGLHFHIGSQIFEIEPFRLA
                     VEALGELRGDWCSILDLGGGLGISYDEADSPPPIEEYVNLKVQAVRDVFGDEVKILIE
                     PGRSLVGTAGVTIYRIGTVKEIPGVRTYVAVDGGMSDNLRPMLYDAKYTAVIADRPDD
                     PAGTTVTVAGSHCESGDILIRDVELANPRVGDILLTPATGAYGHSMSNNYNGMPRPPV
                     IFCEDGEATVVVRRETWDDLLRRDM"
     gene            16341..17627
                     /locus_tag="PLJ64_01645"
     CDS             16341..17627
                     /locus_tag="PLJ64_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92228.1"
                     /translation="MASSARRPINAPPGSPQGTGVPWLGLSILLVLLLILAAFVAYFF
                     FDVGKDEGGDSSSGVTELETGATGGQGAIVGHSNGPLGFPSAATRNTTRISGSDPVDI
                     SMAAALAAYPTSGPGSPPAAVTIIDANDWQSGVAAASLSALPIGSPILLAPSGTLSAT
                     GMDTLAQLDPLGSPDTGENKVFTVGKVAPPSGYDVERLDGNSPAEIAAAVADERAALT
                     DAPPNAFVVVSSEAPDYATPAATWAARSGDVVLYTGKDEVPKATLDVIKDKANKDVPI
                     FVLGPSDVVSSKALKQLDKAGSTVERVGGDDPESAAVELVRFSSGLFGWSLNEPGHGY
                     VLARTDRPMDAIAVTALSTGGTWAALLLTDSSEKLPQVVEDYLLDVKPGYETDPTKAI
                     FSHVWIVGDDSIISVDEQARVDDAVELAPVDTVAEG"
     gene            18391..18633
                     /locus_tag="PLJ64_01650"
     CDS             18391..18633
                     /locus_tag="PLJ64_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92229.1"
                     /translation="MSEAEHIGVLDSGQKITPEDIRELTGAVTPHFAQQVRVRVGRLI
                     SGLDASDPARVFGEREMARLEDLAHHSGQPGSESAH"
     gene            18650..19327
                     /locus_tag="PLJ64_01655"
     CDS             18650..19327
                     /locus_tag="PLJ64_01655"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNC92230.1"
                     /translation="MALKQVAPGVERLGQFPLPMVNVYLAGDILVDAGTTWDRFRIPR
                     QIKDRKLSMITLTHVHPDHQGLARDICRDRDIPLACHVDDVDAMEGRRPVSEGQITSS
                     LLKKIQGSWEGPPHKVSRVLEDGDQVGDFTVVHTPGHARGHVVYFRESDGVTICGDVI
                     RNLNYATGMSGLDEPPEMFTYDPAENRNSIRKLAALNPTVLLPGHGPAVTDMDEFQAW
                     AAALPQD"
     gene            19359..20372
                     /locus_tag="PLJ64_01660"
     CDS             19359..20372
                     /locus_tag="PLJ64_01660"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:TIGR00675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HNC92231.1"
                     /translation="MSGERLFEDQTPSLCFEERVNLLLEPKRPGSPLVLDLFAGCGGL
                     ALGFESAGFETLGFETNPDCVATYRSNLSGDCLEVNLTRDFDFPAKADVIIGGPPCQP
                     FSVRGSQKGNQDARNGFPAFLAAVEQIAPDLVIAENVRGMLYRNREYLDEVVQKLRDF
                     GYSVEVELLNAKNFGVPQNRERVIIVGHRLGWDWPDPSDFPLVTAGEALGDMAFEIPP
                     DSKFLTSSMDRYIAEYERKSGCARPRDLHLDRPARTLTCRNLAGATSDMHRLLLPDGR
                     RRRITTKEAARLQTFPDWFQFCGTELSVFNQIGNAVPPLFSLALAEQAKLSLGLQGVT
                     STV"
     gene            20482..21447
                     /locus_tag="PLJ64_01665"
     CDS             20482..21447
                     /locus_tag="PLJ64_01665"
                     /inference="COORDINATES: protein motif:HMM:NF018339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BsuBI/PstI family type II restriction
                     endonuclease"
                     /protein_id="HNC92232.1"
                     /translation="MINQALEIFDAFGIPPEKTGRRNEAVAMAFIAVAGVTAPSSWQM
                     AEDLDTPRALTTREIIIFANKYLGEERSSGSYDDVRRKDLKRLVEAGLVTNSAPESAH
                     NSPKRGWGINPHFGEAIRSFGSEDWVKELNLAMAGYKTWSEKYSTIREMSRVKVRISD
                     DAEITFGPGAHNELQKQIIEEFLPRYGYGAEVIYVADGENRSLHHDKERLNSLNVFEL
                     KHEQLPDVLAFSEAKDWLYVIEAVTSTGPISLQRHVAISELLADVKCEGVVYVTAFPN
                     REKPFTSFLPEIAWETEVWVASDPDHLIHFNGERFLGPYPVRQPD"
     gene            complement(21510..21887)
                     /locus_tag="PLJ64_01670"
     CDS             complement(21510..21887)
                     /locus_tag="PLJ64_01670"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNC92233.1"
                     /translation="MKLLDTSVVIDFLRGKGAAIRLLTGLAEGHEPLLASEVTRFELL
                     AGVRAGEEASLERFFTAIEWVPVGESICRLAGDLARKFRPSHSGIDMADYLIAATSML
                     ADADLLTTNVRHFPMIEGLAPAY"
     gene            complement(21884..22141)
                     /locus_tag="PLJ64_01675"
     CDS             complement(21884..22141)
                     /locus_tag="PLJ64_01675"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HNC92234.1"
                     /translation="MGRTQVYLGSEELEMLDRAAADTGATRSELIRRAVRRSYGRGHS
                     DPLRIVLETAGAWKSEGSGSDFVDEVRPGGLDERLASFGIE"
     gene            complement(22149..23198)
                     /locus_tag="PLJ64_01680"
     CDS             complement(22149..23198)
                     /locus_tag="PLJ64_01680"
                     /inference="COORDINATES: protein motif:HMM:NF014135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HNC92235.1"
                     /translation="MAALLLLTNGCHRAGVFDYAGWRVAHAGSGERGLLFSLFATMAA
                     ATAALNLDAAVLVMTPVVLAVASRRGIGHEAFSISTVHLANSASLLMPVSNLTNIMVF
                     HETDLSFGHFTALMALPWLGAIAVERLLVPRLARPAEVRSPPELATVKPEVSVWPAAL
                     LGIVLSGFIVSGPLGVEPLAIAWAGAAVMVLGELAGRTAGPVELVRAIRPLFLAALLA
                     LGGLAWLLGDLGLTDLIADLVPAGTGFGSLLLIAFGAAVLANLINNIPATLILLPAVA
                     PAGEGALLAMLIGVGIGPNLAYPGSIANLLWRRVMIEDGAAVSWREFTRIGLVTVPTG
                     IFVATALLCVVYTGV"
     gene            complement(23229..24257)
                     /locus_tag="PLJ64_01685"
     CDS             complement(23229..24257)
                     /locus_tag="PLJ64_01685"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNC92236.1"
                     /translation="MSAMEKVSIGITGETPEDMAAMARLAEAAGFDCGWAIELFRNAF
                     TQTTFLGAKTESMGIGTAIAWGFVRSPMTMAMSAMDIDEITGGRFRLGLGPGVKRLNE
                     TWHNAEYGRPAPHLRETIESVRAIIHAAAASEPVAYKGEYYDLDIKGWFRSTPPVRAD
                     IPIYTAAVQKGMSRMAGDVADGLIGHIICSARWIEQEIVPAFELGLSRSGRDRSSFEF
                     LPSICVAIDDDYQRGLEAARKTLAFYATVKTYLPLFELHDFGQNAADAGAAFRMGDLA
                     GVAEAISDEMVETYCAVGTPDKVKEKVAEVAQFGDAILPGAPTYFIPNEQITEYNEKI
                     IEVFGQTS"
     gene            24379..26148
                     /locus_tag="PLJ64_01690"
     CDS             24379..26148
                     /locus_tag="PLJ64_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92237.1"
                     /translation="MKLAGLAVMAVAFFGSTAAASADPVVFNFDESIINLGGGSGPQG
                     LKLIDPDADPADAPATLNGEVDTATDAFTAAASGFTFPQKTTQVSGQDVVVTITANAA
                     ITGTFDTDTGTADITVPVTVSAAVLGNTCTTSFPLELKTTGTLVEPGGNHDAAPFDPD
                     TGEGSVYSSFTVPNSTGGALCGVVDGQIGGAGDIWLSGTGGIDEGIVTVYPGDDGTSL
                     STTDGGWVHSTSYGPLCALIPGVLCPAMNGTFESANGSSGAADGFLRDTATGLTVTPT
                     GLGQTSTGVWTSPEFTYDGAEGEPAETLSFALARKGTLSDLLSQSSANWQVDLVKSSD
                     SSIIPLIPNGPATNADSWVNIPGSALAADGLTIGDAYRIVITTNVITETLGLAPSGTF
                     DYDEVALTARRATPTGPTGPTGPTEPTGPTGPTEPTGPTGPTEPTGPTGPTSPTGPTG
                     PTGPTGPNKPVTLTKGNAAFNGKRLFIRLYCPREFKPKCNTVSVPVTRKVANKAPKKI
                     KRLSKPMARPIRVTVKSGKWKKVSYLIKPAYRNKVRKMAGKNKRLLAIRDYIKAKKFA
                     GRKFKGKTRTVFHSHKVVIGKAR"
     gene            complement(26236..>26419)
                     /locus_tag="PLJ64_01695"
     CDS             complement(26236..>26419)
                     /locus_tag="PLJ64_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="steroid 3-ketoacyl-CoA thiolase"
                     /protein_id="HNC92238.1"
                     /translation="DMDKVNVNGGAIALGHPLGSTGARLMTTLLHELERQDKKTGLVT
                     MCCGGGIGTATLIERI"
BASE COUNT         4828 a         8580 c         8314 g         4597 t
ORIGIN      
        1 gtgcatgctg gccttcgtca atctgatcca gaaatggggt ctgcgtcatg accgctagtg
       61 gccgactctc caaatacggg ctccggttcg tcgcgctctt ctacctgacc gctctgctgc
      121 tgctgccgat cggcgtggcc ctggtcaaga ccttcgagga cgggatcggt gaagtgatca
      181 aacagatcac ggagccggac gcgatccacg ccctgaagat gtcgctgctc acggtgatca
      241 tcgcggtgcc gctcaacacg gtcttcggca tcgccgccgc ccatgtggtc gtgcggacga
      301 aattccggtt caaatgggcg ataaatgcgc tgatcgacat gccctttgcc gtctcgccgg
      361 tggtcatcgg cctcgccctg gtcctcgtct acgggaccgg cggctggttc ggtgccgacc
      421 tggccgcgat gggcatccag gtcatcttct ccaccccggg aatagtcatg gcggtgatct
      481 tcgtttcgct gccgttcgtg gctcgcgaag taatcccggt gatgcaggaa atcgggaccg
      541 accaggaaac cgccgccgag acgcttggcg ccagcccctg gcagaccttc cgcaagatca
      601 ccctgcccgc gatccgctgg ggcgtgacct acggaatcgt gctgaccaca gcccgcgccc
      661 tgggcgagtt cggcgcggtg acggtcgtct ccggaaacat ctccggcgaa acccagaccc
      721 tgccgcttta cgtatccaaa cagttcgaaa ccttcaactc agtgggcgcc tattcggctt
      781 cgttgatcct cgccgtcctt gcgctgggca ccctgctttc aatgaacctg ctacgccgcc
      841 gagaggacca agatgactga aatcactgac cacctcaacc cggaaagtca cggcatcgag
      901 gtccgggaag tcaacaagga gttcggtgac ttccaggccc tcaaggacgt caacctgatc
      961 gtccccgacg gatcgatgac cgcgctcctc ggaccttccg gttcgggcaa gtcaaccctg
     1021 cttcgcacca tcgccgggct tgaagtgccg accagcggcc aggtgttcat cggcggcgag
     1081 gatcaggcga ggaaaccggt ccggacccgc aacgtcggtt tcgtcttcca gcactacgcc
     1141 gcgttcaagc acatgacggt gttcgacaac atcgctttcg gcctcaaggt ccgcaagtgg
     1201 gacaagcaga agacccgcga ccgggtcaac gagctgatcg atctggtcca gctgaccggc
     1261 ctcgcgaagc gttacccctc gcagctctcc ggcggccagc gccagcgcat ggccctggcc
     1321 cgggcgctcg cggtcgaacc ttcggtgctg ctgctcgacg agcccttcgg cgcgcttgac
     1381 gccaaggtcc gcaaggacct gcgccagtgg ctgcgccgcc tccacgacga ggtccacgtg
     1441 accacgatct tcgtcaccca cgaccaggaa gaggcgatgg acatcgccga acagatcgtg
     1501 gtcatgaaca acggccagat cgagcagacc ggctccgcca ccgacctcta cgaccggccc
     1561 gagaacgagt tcgtgatggg cttcgtcggc gaggtcaaca gcctcggcgg ccggctggtc
     1621 cgcccccatg acaccgagat cctgccggag ccgaccgagg acacgaccga ggttgaaatc
     1681 gaccgggtgg tgcggctggg cttctccggc cgggtcgaga tgctcacggt tccggacaaa
     1741 aaggaggtct gggcgcagct gacccgctcc gagctggaga ccctcagcct cgaagcaggg
     1801 acgagggctt acgtcaggcc acgggccgaa gcggtgacca aggaagacac gggcgcgctc
     1861 gaggaagcgc tcagggaaga gagtcctgcc accgacacgc ccattcccgc agagccagca
     1921 ctgccggtct gagcccttcc cccatctcgg tgaggctgta ggtcaccttc acctgggggc
     1981 catcttcgac ctcgcgggtc atcaggcccg cgtcctcgag ctcgcgcagg cgctgggaga
     2041 gcagccggtc cgaaagcccg gggatcgaac gcttcagttc cgcgaaccgc aggggcccgt
     2101 catccagggc ccagatgatc gcgccactcc agcgcttgcc gatcagttcg atgcccgcgt
     2161 gaaaatgggg gcaaacttcg gtcagggccg gtcgggtggt acggccgtcg gccgtttgcc
     2221 aggtatcggt tgaaccggtc accgttcttt cactttagcg gagccggaat gtgggaaaat
     2281 ccggatttcg accgacggga ggagataccc gcctacgcgc aaggtccggt gtcgacttcc
     2341 ttgccgctga cttttttgct gatgtcggca aggacccggt tcgggattgc cagcccgccc
     2401 tgcggccctt ccgtggttgc ggcgaacacc gtgcccagca cctgcccccg ggaatcaacc
     2461 agcggcccgc ccgagttgcc gtgccggacg ttgcctctga gggccagcat cgaacgctcg
     2521 aatgaaccgg agccatagga gtcctggcca atcaggatgc gggtctcgcc ggcccgggcc
     2581 ggggtgatcg tcagcgggcc atcgttcggg tatccgatca ccgccgccgc ggtgccacgg
     2641 cgagcccggg cgagagccgg cagcgccggg aggtcgacgt ccgcatgcag gatcgcgatg
     2701 tcgttgcggg tgttgaaggc gatcggcgtg gcggacgcgc tctgcccgtc attggtctgg
     2761 atcgtcgtgt cgtcctggcc tgccaccacg tgggcattgg tcacgaaagt gtcgggtccg
     2821 accgcccacc cggaacccat gacgccaaag ccgttgcagg tgccggagac cttgaccact
     2881 gattcggccg ccttctgcac cgctgccttg ctgccggtgc cctggtccgg tttggcgatc
     2941 tcaccggtcg aagcggcgat ccgggggaag ggatcgatcc ggtggagggc ctgcatcaaa
     3001 ggccccgacg ggggcatcac gtcgttgacg gcaccgacca ggagtgactt ctggacgtcc
     3061 cgacgcaggc cgtccgaact cgggtagtag acggcgaccg cagccaggac ccagacgatg
     3121 cccagcccga cggcagcgat cagaaccgcc ccgccgatcc cgtcgatcac ttcccagaat
     3181 ggatggctga ccgtggaatc ccgtacccgc tcgccgagcg tgaccgagag ggccagggtt
     3241 atggaaccga cgaagatcgc cccgaccagc gcggccagtg gcgcgtaggg cgagttcgag
     3301 ccatggtcga gaaagaccgg ggccagccgg gatcccagga tggccccgac cgcgagtccg
     3361 gccagtccca gcgcaccgac gatcaaccct tgacggaaac cccagagggc aagcaggaag
     3421 gtgaaggcgg cgatgcccca gtccagcgct gtcacccggc caatttagcc cccgggcggc
     3481 accgatggtc agccgcaaca tctaaccacc ggcgaccccg gtatttttca gcccagatgg
     3541 ggcgtcctcc ttcacgccag gaacggcaaa aacgactcaa gacgagggct ttgcctctgg
     3601 tcggagtcgc gctgatctct ttcatcgtcg gaatggtcaa gggatgcccc ggcagcccca
     3661 accgtgatgc cgccgagcag tacgtcgagt actgggccga aggcaattac gtcgcgatgt
     3721 acgacatgct cagcaccaag tcccgggaag ggatcaccca ggagcgattc atcgccaggt
     3781 acggggaagc ttcggccgag gcgacgatcg agagcatcga acaaagcgat gccgaaggcg
     3841 atgaaaccaa cgccgaaatc ccgatggaag tggacactct cgctttcggg acaatcgacc
     3901 agtcgctcaa ggtcaccttc ggttcggacg gaatcgcctg gcagaacagc ctgctcttcc
     3961 ccgggctcga actgaacgaa gagctgacca gggtcaccaa gctgcccggc cgggcgaaga
     4021 tcctcgccga agacgggcag gtgatggccg agggtccggt cgacgcccgc gagcatccgc
     4081 tcggagactc gatgatcgac gtgaccgggg tcaccggacc gcccgaccag gaagagggcc
     4141 cggatttgac cagcctcgcc ctcggttacg aacccggcga ggacaccggc atcagcgggc
     4201 ttgaactcgc ttacaacgcg cggctcaccg gcacccccgg gggcaccctg ttcgccaagc
     4261 cgatcgaggg tggaaccgat ggccaggggc gcgagctcgg cagcggcacc ccggccccgg
     4321 ccaaaccgct gaagaccacg atcgacccgg atctgcagga gtcggcggtg gcaaacctcg
     4381 gcggggtctc cggcggcgtg acggtgctcg acgcgaagaa gggcttcatc aaggctctgg
     4441 ccggttccgc ctattcggtg ctgcagccgc ccggatcgac gatgaagatg gtcaccgcca
     4501 ccgcggccct ggaaacgggg gccgcgacca tggattcctc atacgactac gtgactgagg
     4561 ccccggccga cgggcgcatg atccagaacg cccacgggga ggtctgcggc ggcaccttca
     4621 ccgaggcttt cgccaagtcc tgcaattcgg tcttcgcccc cctggccgag cagatcggcc
     4681 ccgaacagtt cacccggacc gcggagctct acggcttcaa ccggcccccg caggtctgga
     4741 atgacgaggg tctggccgcg gtcaatccgc cgactcccac cctgccggaa gccgatgact
     4801 acgacagcga cctcggcgtc tccgggatcg ggcagggccg ggttcaggcc acgcccctgc
     4861 tgatggcaag cgtcgcccag gcgatctcca acaagggaat ttccctgccc accccgataa
     4921 ccaccgaagc ggacctgcag ccggaaatga agccggtccg ggtgaccagc cagaaggttg
     4981 cctcccaggt cgccgacctg atggttgccg tggtcacctc gggaaccggc atcgccgccg
     5041 ccattcccga agcccaggtg gccggcaaga ccggcaccgc cgaagtggga ccgagcggcg
     5101 aggtggacgc gaacggtgat gacatcctga tcgaggacgc ctggttcgcc ggttttgccc
     5161 cctccgacaa gccgcagctc gcaatctgcg tcaacgtcct cgacgcctcc ggtgacggcg
     5221 gcacggtcgc cgccccgatc gccgccgcca tcctctccga cggcctttag tcctctcgcg
     5281 acaagggacc ccgcgagatc ccactttgtg ccacataccg gcacaaaccg ggcactcgac
     5341 ggctccgccc cagaccgggc actcgacgcc tccgccccag accggccccc ggtgggtcgg
     5401 ttttccctca cctgccccgg aaatccgacc caccacctgg cgcgggaccg gtccgcaacg
     5461 gttcgtgggt cggatttccg gtccagacgc ccgttatccg acccacctgg gtggaaccgg
     5521 gcaggcgacg ggggaacttc aggtgacccg gagttccgtg gtacctccaa agccgggtcc
     5581 aggccccgtt ttcgggaggt gggtatggat ttcgtctata taggcgaaat ccatacccac
     5641 ctcccggggt tcgggtgggg aaccggtcga gatcggagtt gggtgcgcta tccggcaaga
     5701 aacgagatct cgaaggatgc cgggccctgc cgccgcgctg ggctcttggc gctgcggggc
     5761 ccttggcgct gcggggcccc cggcgctgcg gggcccccgg cgctgcgggg tcttggcgtt
     5821 gcccgggggg atgggcgctg cggggagttt tattcgggtt gggccggggt tccgaagaag
     5881 actgcggcga gggaagtgac ggcgccgatc gtgaggagta ctcctccggg ggccggcagc
     5941 cagaaggcca ggccgtagcc gatcagggtg gccagggcgg cgagggcgag gaccgaccac
     6001 cactcggcca tgaccgggcc cggctggcga ccggccggag cggcgaggac gactccgatg
     6061 atcagaatcg cgacgccgat tccggcgatc acgtccgaaa acggtcccag cagggccagg
     6121 gctgctccga gcccggccgc aatctgaccg gccagcgccg gcccgtggtc cgccagcctg
     6181 aggtggccgg gaccgtctga cacctagaga tcgaggtcga aggtggcggg gccgtcttcg
     6241 ccgtcgatcg tcagctcgac cggccgctgt tcggccgact cgcgatcaat caggaagagc
     6301 agcatcgagg caccgatcgg tccgacctgg gcggtcgaat cgacctgcgg gatgttgtcg
     6361 tggggctcga cggttgcgcc gggctgaagg gcgtagacgc tcttgctcgg gatgttctcg
     6421 tacttgcgac cggcggtcgt ctcgatctgg aagctgtccg gaatcgtctg gggatcgtca
     6481 ctgtcgttgt gaacgtggac gaagaccccg atgtacatct ggtcaggacc cggggccggc
     6541 ttgccgacca ggtactgcga atcctcgatg tcgttcctgt tcagcggccg ggtgaagagc
     6601 acgttgtact gcagcgggcc gagctcgacg atttcacctt cggcgagacc ctccttctcc
     6661 tcatggctgg caccgcaagc ggcgagcgcg agcccggaga tagcgactac gagggccagg
     6721 acgaggccac gaaacaggtt gctcttcgaa agtctttcca tcggtgcggg agtttattcg
     6781 gccttatccg gacgtgctga gcggttccta ggcgacaccg gtcgcgtgtc ggttgccgcc
     6841 ggcgaccgga acctcgacct tctccctgcg agccgactca atcgcctccc gaagacgctt
     6901 gccgaccttg tccagacggc gcctgtgcca gcccgcgccg ccggtcaggc ggttgtgaac
     6961 ccgggagaat ccggccggca cgatccagaa ggtgatccgc acccgggtta ccccggttga
     7021 gatctcttcc atctcccact cggtgccggt cacgttgcgg ttgtagctgc cggtggaccc
     7081 gcgttcggaa atccgccggg gggcttcggt cgcggtgatc gccgaatcgg cccaggcctt
     7141 cttgcgcttg aactgccagc gggcgccagc gccgatgccc cgcgaatcca gccgcaggag
     7201 ccgaaagttc tcgaccgagt cgccgtaaag aaacgggcgg gtatcgaagt ccaggaggta
     7261 gtcgaagatc tcttcgcgcg gcgcgtcgat gtcgacttca gtgaatacgg gacccatcga
     7321 tccggattct acgacctgcg tgccgatgcg tgcccgaccg gcgatagggt gtgccggtgt
     7381 ccacgacgat cgcgatcctt tcccagaagg gaggaaccgg gaagaccacg ttgacgcggt
     7441 cgctctccga gggcctgcgc cgggcgggac tttccgttct cgcgatcgat gccgaccccc
     7501 agggaaacct ttccgagtac ttcgggatcc cggccgatgc cttccccacc ctcggtgaag
     7561 tgctggccgg ccgggaacgg gcggtcgatg cgattcacac cggccccggc ggcggcgagg
     7621 tgatccccgc caacctcggc ctggccgagg ccgaactgat gttggccggc aagatcgggc
     7681 gcgaactcac cctgcgtaac gccctgcggg agccgaaacg gcgttttgac gtgatcctga
     7741 tcgactgccc gccgaccctg ggcctgctca ccgtgaacgc cctggtcgcg tccgacaagg
     7801 cgatcctctc gacccaggcc gagcatttct ccgagcaggg ggccgagcag gcgatggaag
     7861 tgatcggcct ggcccgggag aacctgaatc cggagctcga gttgctcggg ctgctgctca
     7921 acatcgccga catgcggacc aatcacgccc gcagcaccct ggagaccatg cgcgagcaat
     7981 tcggggacct cgtctttgac acggtaatcc ggcgttcgat cgtctatccc gaatcagccc
     8041 gggcagccaa gccgatcttc gattaccgca agcgaaagtc nnnnnnnnnn nnnnnnnnnn
     8101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn gatcaggccg atccggacga gcaggtccat ctcagagctc
     8221 ggggacgtcg gcgccgaggg ggctgaagcc gactcgcctt acctcggccc agccttcgca
     8281 acgctccctc aactgctcga ccactccccc ggtttcctgc cagttggtcc agaccaggac
     8341 ggtgggtccg gccccggaga tcgtcgcgcc gatcgccccg atctcgccgg ccacttcgac
     8401 gatctccatc gatttcggga agaggtggcg gcgccgttcc tggtggagac ggtcgtggag
     8461 acccattccg atcaggtcgg agtcacccct ggccaggccc agggccaggt gggcggcgga
     8521 ggccgtgttg gcgaccgcgt ccttgatcgg gatctgttcc gggatcgcct cgcgggcctt
     8581 gcgggtggaa acctcgacct ccgggatgac caggatcccc tccaggcctt ccggcgcttc
     8641 gatcacggtt gcggtcagtc gcgggccgcc ggtgcagatc acgaagcccc cgcagatcgc
     8701 ggcggcgacg ttgtcgggat ggccctcgag agccgtggcg cgttcaagca gatcctgccg
     8761 ttcgagcccg agttcaaaca ggtggtcggc cgcgaccagt ccggcgacga tcgcggcggc
     8821 gctcgatccc atgccgcggg cgagggggat ctcgccgccg acccggaagg aaatgccgtc
     8881 cgcgggctgc agggtttcga aggcgcggac cacaaggttg ctgcgatcga gcggcacgtc
     8941 gagcgcgtcg cagctgaccg agaaggtccc cgtctccttc acctcgagtt cgaggtgaag
     9001 atccaaagcc accgccatca cgtcgtagcc cggtccgaga ttggccgacg aagcgggaac
     9061 ccggacgagt cgatgccgtt cagtcacccg gcaaaaatac cttgctaatg attgaggggt
     9121 gagttcgtcc tccgccaatc cacctgatcc gtccccccct gcgcttgaaa tcaccgatct
     9181 ggtcaagcgt tacccgaccg gtgtcgaagc cctgaagggc gtttcgctcg agatcgccga
     9241 cggcgagttc ttcggcctgc tcggtccaaa cggggcgggc aagtcgactt tgatccattg
     9301 ctcgaccggt cttgccagcc cgacctcggg cgagatccgg gtcttcggcc acgacgcggt
     9361 cggcgactac cggcaggccc gcgaggcggt cgggctggcg ccgcaggaac tgaacatcga
     9421 ctggttcctc acggtcgagg agaccctcga cttccacggt ggctacttcg gcatgggcaa
     9481 gaaggaccgc aaggaacgag tcgacgagct gctcgaatcc ttttccctgg tcgataagcg
     9541 aaacgaccgg acccgcgacc tgtccggcgg catgaaacgg cgggtgatcc tggcccgggc
     9601 gctgatgcac cggcctcgcc tgctgatcct cgacgaaccg accgccgggg ttgacgtcga
     9661 gcttcgcctg gagctctggc attacgtcca gcaggtcaat tccgaaggca cctcgatcct
     9721 gctcaccacc cattacctcg aggaagccga gcagctctgc agccggatcg ccttcatcaa
     9781 cgggggcgag atcgtcgacc acggttccag cgacgagctg gccgagcggt tcggggtggg
     9841 cagcctcgag gacgcctacc tggaactggt cggccgcaag gagctttccc gtgcccatgt
     9901 cggaatcgaa gcggaggacg gccagtgagc ccgtcgatgg tgcggctggt ctccctgacc
     9961 aggcgcgagg tcagccgctt tctcaagatc ccccgccaga cggtcggcgc accgctgctc
    10021 gagaccttcc tttacatatc gatcttcggt gccgcgctcg gttcccgggt gggtgacctg
    10081 cacggctacg aatacatcgt cttcgtggtc cccggcctga tcatgatggc cttcgcgatc
    10141 aacgccttca ccaacaactc gtcatcgatc cttcagcaga agttccaggg ggcaatcgat
    10201 gaccagctct cctccccggc ctcgcccggc cagttgctcc tggccttcac gctcgggggt
    10261 ttcctgcgcg gcctcttgat ctcggtcgtc acgttcctgg tcgccagcct gctgatcgac
    10321 ctgccggtca agcacttcta catcctcatt cccgccctgt ttctggtcgg ctggttcttc
    10381 gccagcctcg gtgtgctggt cggggtcagg gcggaacagt tcgacgacgt ctcgtttgcc
    10441 cagaccttca tcctccagcc gctgatcttc ctcggcggcg tgttctactc ggcctcgctc
    10501 ctgcccgagc cctacgaaac cctgacccat ttcaacccgg tctactacat gatctccctg
    10561 gtccgttacg ggtttctcgg ggtcgccgac atcagcgtct ggctttcact tgccctgctc
    10621 accgcggcca ccgccgcact caccttcgcc aactaccgga tgttcaagtc cggctacaag
    10681 ctccggagat aacgacggat cagcgcctga tcctgatgac caccttggcg accggggact
    10741 ggttgccgag cttgtcgcgg gtcctgatct tgatcaccgt cttgcccttg cgggtgacct
    10801 tgcgggtctt cggcacggat ttcgggttga gcccgacgcc ggcggtcccg tccggcagct
    10861 tgccgtccgg cttgccgatc accttgaccc ggaccgggcc cctgaactgg cctttgacct
    10921 tgtgaccctt gagcacgatc ttcggcttcg ccggtccggt ccggtcgatc ttgaccgcga
    10981 cgctggcttc cgagaacggg gtcacgacgg tctccggctc ggagatgttg gtgccgggca
    11041 gcacggtggc gctgttcacc cggaagtagg cggttccctg ggcggcatcc gcaatggtcg
    11101 cggtgctgtc gctcaccgac ttcgtcttga cctttgccca ggcgttgggc tttttggtgc
    11161 tggcccgctg aacctcgaag gtcgtgtcgg gggcttcggt cccgacccag acgagctgga
    11221 agccgccctt gttcgatgtt ccgccgatca cgctcggcag cgacggccgg agccgcgcgt
    11281 attcacgtga gtcccccttc aaccggtcgg catcctcggg caggatgaag ccggaatcgg
    11341 ccagggcgtc ggccgcctcg gagtactgcg ccaggtagtc ggcctgatcg gtgtaaagcg
    11401 aatgaagcag ctcgggcgag aacgggaccg atgaaccgaa cagcccgcag aaggcagaga
    11461 agattgagcc tccgggagcc gccgagttga tgccgtcatt ggtcgcggtg ggcaccgtca
    11521 cgtcggggta gcggattgcg cccagcgcga tcccgttctc gtcgcgttcc agctggttgg
    11581 ccatgtccgg gggactcgcc tggtactcgc cgcgtggcgc gatcggcggt gcggcgtcgt
    11641 cacccaccca gccgaccagg tcgctgatcg cggcgttctg ggtgtagggc cacttgattc
    11701 gcgacagcgg gaacttgacg cagttcgccg tcacggtggc gaagtcgcgc gagaacaccg
    11761 ggttcagatt gtcgaaggcg acccggggca ggtgggacgc cgccgcgacc tcccagtggc
    11821 gaatcgaacc ggtgtccgga tcggacgcga cggttcgcgg aacccggttc tcggtctcgg
    11881 tgatgatccg gatcgccttc tccccgcggt cgccgcggag cgcggaggtg cagaccgtca
    11941 gcagataggc atccacgact tcatgaatcg gctcgatccg gttgtaatgg gtgaccagac
    12001 gaccgcagga ctgtgattca cccgaggcga tcaccatcga gggcgtgatc ccgcccaggg
    12061 gatcgacgcc gttgcggctg cccttcagtg ccttgacgac cgaggagtag acgtcatatg
    12121 agagggcgtc ggcatccagg ccggtaccgg tcggcacggt gtcgttgccg ttgaccgtca
    12181 gcgtcccgta acggtcgctg tcgaaggcct tcagggaggc gacaccgacg ttctgggcgg
    12241 tcaccccgac gaaggtgtaa ccgtgcttga gcaggaagta cgggtcaccg aaccagttga
    12301 actcgacgtc ctgggtcgag gtgacgttgt tccattcggc gatgaccacg ccgttcgcgt
    12361 cggccggatt ggcggggcgc ctgaccacga tccgggtctt gaacgggtac ttgccgtcat
    12421 cggccgaacc accggtcgtg atctttgccg cgggagtgtc gatcgctccg gtcatgtcgt
    12481 accggtaggc gtcaccggcg aggaaatact cgttctcgac gtagccgtac ttgtcaaggt
    12541 cgatgtcggt ggccagaaac ggatgtgaat cggcggtcac cgcgatcggg ccggtcaccg
    12601 tcgcggtcgg aatctggctg agatcagccg acgccggacc ggccataacc agcatgaaaa
    12661 atgacgccgc aatcgtcgag acgacggctg agaacttcct gcctgaaccc atttacctct
    12721 ccctctcccg ttgaaaaagc tacgggagaa tctaccgcag ttggcgccta gccaaagaca
    12781 gcttcttcga ccgcgccgat ttcgttcggg cagttgatca ccgagggggc cttgccgaga
    12841 gcggtgtccg gatccttgag gccgtggccg gtgagtacgc agacaaccgt ctcgggcttc
    12901 tccattccct ccacggtcgg caggccgtgg ttgagaattc cggccaccga agcggccgag
    12961 gccggttcgc agaacacacc ttcgttggcg gcaagccagc ggtaggcgtc gaggatctgc
    13021 tcgtcgctga ccgcggcgac ccgtcccctc gaggagttga aggcgttcat cgcctcttcc
    13081 cagcgggccg ggttgccgat ccggatcgcc gaagcgaccg tctccgggtg ggcgaccggg
    13141 gcgccgtcaa ccagcggagc cgagccggcg gcctggaagc cgtaacagat cggtgcggtt
    13201 cccctttcct gaaagccctt ccagtaggcg gtcacgttgc cggcgttgcc gaccgggatc
    13261 gccagtgcgt ccggggcctg gccaagctcg tcgatgagct cgaacgcggc cgtcttctga
    13321 ccttcgatcc tgtacgggtt gaccgagttg accagctcga tcggattccg ttcggccagc
    13381 tgccggacga tggtcagggc atcatcgaag ttgccttcga gggcgaccac ccgggccccg
    13441 tgcatgagcg attgggccag cttgccgatc gcgatcttgc cttcggggac gatgaccgcg
    13501 ccgcgcagcc ccgcccgcac cgcgtaggcg gaggcggaag cggcggtgtt gccggtcgag
    13561 gcacagatca cggccttggc accacgcccg gccgcccggg aaaccgccac ggtcatgccg
    13621 cgatccttga aggagccggt cgggttcgca ccttcgacct tcagccagac ccgggccccg
    13681 acccgctcgg aaaggcgcgg cgcctcgacc agcggggtcg agccctcgtt caggctgacg
    13741 accggatcac ccggatccag cggcagggcg tcgcggtacc tctcgataag tggctgtggc
    13801 atggcttcct aacctgtgaa ttcttcttcg atgacccgga tgaagcgcgg ccccgacttg
    13861 agcgagtcga gctccgcgat ctgcgcgacc gccgccttga attccgattc gaggcaacgg
    13921 tggatgacca tcaccaggcg ggcgtcgtcg ccgagcccgc gctggacgac gctcttgact
    13981 gacacgccac cgtcggaaag caccttggcg acttcagcca ggacccccgg ttcgtcggca
    14041 acctcgaggt ggaggtaaaa ggatgaagtg acgtcggcca gcaccggcag gtcgcggtcg
    14101 gcggtgtgga cgcgggcgtc gccggccgcg accgaaacca cgtcaccgag cacggcggca
    14161 gcggtctgaa gaccaccggc gccggggccg gacatggtca cctcggtgat ttcgggcgcc
    14221 tccaccatca ccgcgttgaa cggaccctcg atcggcgcca ggggatggcc cgggtaaagg
    14281 aagcagggaa agacccggac ggtgatcccc tgctccagct tctcggcgac cccgagcagc
    14341 ttcagcgaaa ggccgagctc cttggcgtag gcgagatcat ccggctggat gtcctcgatc
    14401 ccctcgaagt cgacctgatc aagggtgacc ggggtgtgaa aggcgagccg ggcgaggatc
    14461 gccatcttcg cggcggcgtc ggccccgttc acatcctcgg tcgggtcggc ctcggcgtaa
    14521 ccgagttcct gggcccgctt cagcacctcg gcgtaggagg caccggtggc ggccatctcg
    14581 gaaaggatga agttcgtcgt cccgttgacg atgccgaaga ccttgctgaa ctcgaccgcg
    14641 ccgaagcttt cctgaaccac gcggatcacc ggcacgaccg cagccactgc ggcctcgaag
    14701 cggagctgga ccccgccttc ttcggcggcg gcgaagagct cggccccgtg ctgggcgagc
    14761 agttgcttgt tcgcggtgac caccggcacg ccggccctga gcgccgccag cacatgttcg
    14821 cgggcggtgt cggtgccgcc catcaactcg acgatcacgt cggcatcggg caggatctcg
    14881 ccgaaatcac cctggctgcg ggtcaggacc ccggtcacct ccggccgacg gccggtcgcc
    14941 gcctcgacct ggtcggcccg ttcggcaaca agttcatcga aggcggagcc gacggttccc
    15001 cgtccgagca ggccgatgcg caggttctcg ctcacatgtc cctcctgaga aggtcatccc
    15061 aggtttcgcg tcgcaccacc acggtggctt cgccgtcttc acagaaaatc acgggcggcc
    15121 gcggcattcc gttgtagttg ttcgacatcg agtgcccgta ggcgccggtc gccggggtaa
    15181 gcaggatgtc ccccacccgg gggttcgcga gctcgacatc acggatcagg atatctccgg
    15241 actcgcaatg tgaaccggcg acggtgacgg tggtcccggc ggggtcatcg ggccggtcgg
    15301 cgatcaccgc cgtgtacttc gcgtcataga gcatcggccg gaggttgtcg gacatgccgc
    15361 cgtccaccgc gacataggtc cggaccccgg ggatctcctt cacggtcccg atccggtaga
    15421 tcgtcacccc ggcggtacca accagcgagc ggcccggctc gatcaggatc ttgacctcgt
    15481 ctccgaagac gtcgcgaacc gcctggacct tgaggttcac gtactcctcg atcggcgggg
    15541 gtgaatcggc ctcgtcgtaa gagatcccga ggccgccgcc gaggtcgagg atcgagcacc
    15601 agtcgccgcg caactcgccg agtgcctcga ccgccaggcg gaagggctcg atctcgaaga
    15661 tctgggagcc gatgtgaaaa tgaaggccga ccagattcag ggtgtccgag gcaagcaccc
    15721 gggtgatcgc tttggcggct gtaccgtcct ccagcccgaa gccgaacttc gagtcaagct
    15781 ggccggtctg gatgaaggag tgggttgacg gtttgatgcc cggggtgacc cggatcagca
    15841 cgtcctgctt gcgcttcagc acccgctcgc atcgatcgat ttcgtcaaac gagtcgacga
    15901 tcaggtggcc gacccccgag ttgccggcga gcaccagctc ggcgtcggtc ttgttgttgc
    15961 cgtgcatgta gatcctggcc gggtcgaaac cggcggtaag ggccatggtc aactcgccac
    16021 ccgaggcgac atcgaccgaa agtccttctt cggcgaagag ccggtaggcg gccgtgaccg
    16081 gcagggcctt gctcgcgaaa agcacttcga agtgatcggt atgtgcggcg aaggcacgct
    16141 gataggcccg ggcccgttcc cgcatttcgg ccgtggcgaa gaggtaggac ggggtgccga
    16201 actcgcgggc gagttcgatt acgtcgcagc ctccaatctc gaggtggccg gcgtcattgg
    16261 tggttgtccc aaccgggtat ggcatcgccg ccattcttac gatttccggt gaacgacacc
    16321 gtttggatac ggttcacgcc gtggcaagct ccgcaagacg gccgatcaac gcaccgccag
    16381 ggagtccgca gggcaccggg gtgccctggc tcggcctctc gatcctgctc gtcctgctgc
    16441 tgattctggc cgccttcgtc gcctatttct tctttgacgt gggcaaggac gaagggggcg
    16501 attcctcctc aggcgtgacc gagctggaaa ccggcgcaac cgggggccag ggcgcgatcg
    16561 tcggtcactc gaacggcccg ctcggtttcc cctcggcggc cacccgcaac acgaccagga
    16621 tcagcggcag cgatccggtt gacatttcga tggccgccgc gctggccgcc taccccactt
    16681 caggacccgg ttcgccaccg gcggcggtca cgatcatcga cgcgaacgac tggcagtcgg
    16741 gagtcgcggc agcttcgctc agtgcgctgc cgattggctc gccgatcctg ctcgccccgt
    16801 cgggcacgct ttcagcgacc ggcatggaca cgctggccca gctcgatccg ctcggttcgc
    16861 ccgataccgg cgagaacaag gttttcacgg taggaaaggt cgccccgcct tccggctatg
    16921 acgttgaacg gctggacgga aacagcccgg ccgagattgc cgctgcagtg gcagacgagc
    16981 gcgccgccct gaccgatgca ccacctaacg ccttcgtggt cgtctcctcc gaggctcccg
    17041 attacgccac tccggccgcg acctgggccg cccgatccgg agacgtcgtc ctctacaccg
    17101 gaaaggatga ggtgccgaag gccaccctcg acgtgatcaa ggacaaggcc aacaaggatg
    17161 tcccgatctt cgtgctcgga ccgtcggatg tggtctcaag caaggccctg aaacaactcg
    17221 acaaggccgg ctcaacggtc gagcgggtcg gcggcgatga ccctgaatcc gccgcggtcg
    17281 agctggtccg cttctcgagc ggcctcttcg gctggagtct gaacgagccc ggacacggct
    17341 atgtcctggc ccggacagat cgcccgatgg atgcgatcgc tgtgaccgca ctctctacag
    17401 gtggtacctg ggcggctctg ctcctgaccg attccagcga gaagctgccg caagtggtcg
    17461 aggactatct ccttgatgtc aagcccggct atgagaccga cccgaccaaa gcgattttct
    17521 cccacgtctg gatcgtgggc gacgactcga ttatctcagt ggacgaacag gcgcgtgtcg
    17581 atgacgcggt cgagcttgcc cccgtcgaca ctgtggctga gggatgaacc cgaaacgttg
    17641 ggactacttt cgtccacttt tcggggacaa tgttgggcta ccctgagatc ggggattctc
    17701 agttacaaag gcgcctgcgt agtcggctac gcggacgtct ctcaatgttg ggacttgggt
    17761 gtgcggccag aaacgaatcc gaccgcacac ctagtcacta ctgacaggtc acgtcagacg
    17821 aatcgacccg attgaacttt cccgggtcag gtcgttgccc tccagggttc atgcaagcaa
    17881 ctccagtggt tgagccgccc tcactcaaga gcaaccgcac atccttcctg cagttgttct
    17941 tggcagacca ttgagctcct acgttgacca cccctacaca gggctcaata acattcttga
    18001 cgcctcgata gagccgctga gaccaggaac aaaacctaaa ttccgcgcat cccgaaccat
    18061 ctgccgcgct caccgcaacc gcttgattct ctatagcgat acctcggttt tcggaagctg
    18121 aagcagttga ttggccgtgc gttgtaattg cgcccgcgaa gcccaataga cctaaacaaa
    18181 ctagaacggt cgccacaaga cgccgatgtc gcaaatcggt gtaactcata cgaactcctc
    18241 tcgtaggttg accatcagag gctaccgtga agggccggag acatgcggca tatggaaaag
    18301 tcacgtcgtc agatactcag gcgcatacca ctgaaaaatg gggtctgcgc ccagacacga
    18361 cacgtggaaa tagtggagaa tgccccaaag atgagcgaag ccgagcacat cggagtattg
    18421 gactccggcc agaaaatcac tcccgaggac atccgcgagc tgacgggtgc ggtcaccccg
    18481 catttcgcac agcaggtgcg ggtgcgggtc ggacgcctga tctcgggcct cgatgcttcg
    18541 gatcccgccc gcgtcttcgg cgagcgcgag atggcgaggc ttgaagacct cgcccaccac
    18601 agcggccagc ctggctccga atcggcccac tgaacccgga attcggaaga tggcactgaa
    18661 acaggtcgca ccgggggtag agcgactggg ccagttcccg ctgccgatgg tcaacgtgta
    18721 cctggccggg gacatactgg tcgatgccgg aacgacctgg gaccggttcc ggatcccccg
    18781 ccagatcaag gaccggaagc tttcgatgat caccctgacc catgtccacc cggaccatca
    18841 gggcctggcc cgcgacatct gccgcgaccg ggacatcccg ctcgcctgtc acgtcgatga
    18901 cgtcgacgcg atggagggcc gtcgtccggt ttccgaaggg cagatcacca gttcactcct
    18961 gaagaagatc cagggttcat gggaaggccc gccccacaag gtctcgaggg tgctcgagga
    19021 tggcgatcag gtcggcgatt tcacggtggt ccacaccccc ggtcatgccc gaggccatgt
    19081 ggtctatttc cgcgaatctg acggcgtgac catctgtggc gacgtgattc gcaacttgaa
    19141 ttacgcgacc ggaatgtccg gtctggacga gccgccggag atgttcacct acgacccggc
    19201 cgagaaccgc aattcgatcc gcaaactcgc cgccctcaat cccacggtcc tcctccccgg
    19261 ccacggcccc gcggtaaccg acatggacga attccaggcc tgggccgccg ccctgccgca
    19321 ggactgactg tccgcaatgg tccgtaaagt gtttgcccgt gagcggcgaa cgtctatttg
    19381 aggatcaaac tccatccctc tgctttgaag agcgggtcaa cctattgcta gagccgaaaa
    19441 gaccgggcag tccgctcgtg cttgacctct ttgccggatg tggtggcctt gcccttgggt
    19501 ttgaatctgc ggggttcgaa acgcttgggt tcgaaaccaa ccccgactgc gtagcgacgt
    19561 accgttcaaa cctcagcggc gattgccttg aagtaaatct gacccgggat tttgactttc
    19621 ccgctaaggc cgacgtgata atcggcggac cgccttgtca accgtttagc gttcgcggat
    19681 cccagaaggg aaatcaggac gcgaggaatg gcttcccggc attccttgcg gccgtggaac
    19741 agatagcgcc tgatctggtg atcgccgaga atgttcgcgg aatgctctac cgaaatcggg
    19801 agtatctcga tgaggtcgtt cagaaactga gggacttcgg atactccgtc gaagttgagc
    19861 ttctgaacgc gaagaatttt ggagtgccac agaatcggga gcgggtaatc atcgtagggc
    19921 accggcttgg ctgggattgg cccgacccgt ccgactttcc cctagtgacg gctggcgaag
    19981 cacttggcga tatggctttc gaaataccgc ccgactcgaa attcctgact tccagcatgg
    20041 atagatatat cgccgaatat gagcgtaagt ctggttgcgc gaggcccagg gatttgcatt
    20101 tggatcggcc cgcaaggaca ttgacgtgcc ggaaccttgc aggcgccacc tccgatatgc
    20161 accgattgct tctcccggat ggtcggcgac gacgaatcac gaccaaggag gccgccagac
    20221 ttcaaacttt tcccgattgg tttcagttct gcggaacaga actttcggtc ttcaaccaga
    20281 tcgggaacgc cgtaccccca ttgttttcgc tcgctctcgc tgaacaagcc aagctttcgc
    20341 tgggcctcca aggcgtgact tccaccgtct gagtgtctca acgggatatc ggatcaagtg
    20401 gcttataacc tgtcccccac ttcgacagtc cgatcaatca gcagtaaaca gttccgcaag
    20461 aagtcatctg aaattcagga cttgatcaac caggcgttgg agattttcga cgctttcggg
    20521 attccgccgg agaagacggg gagaaggaac gaagccgtgg cgatggcatt cattgctgtt
    20581 gcgggcgtca cagcccctag cagttggcag atggctgagg accttgacac gccacgagcg
    20641 ttgactactc gggaaatcat aattttcgcc aataagtact tgggtgaaga gcgttcgtcg
    20701 gggagctatg acgacgtacg gagaaaagac ttgaagcgcc tcgttgaagc cgggctagtg
    20761 accaatagcg cacccgaaag tgcccacaat agcccgaaga ggggctgggg aatcaatccg
    20821 catttcgggg aggccatccg gtcgtttgga tccgaggact gggtgaaaga actcaatctc
    20881 gcgatggccg gatacaagac atggagtgag aagtactcaa caataaggga gatgagccgc
    20941 gtaaaagtcc ggatctctga tgatgcggag atcactttcg gaccgggcgc acacaacgag
    21001 cttcaaaagc agatcatcga agaatttctc ccacgatacg gctacggcgc cgaggttatc
    21061 tatgtggctg atggggagaa tcgcagtctc caccacgaca aggagaggct caattctctg
    21121 aacgtgttcg agctcaagca cgaacaattg cctgacgttc ttgctttctc agaggcgaaa
    21181 gactggctct acgtgatcga agccgtcacc agtaccgggc cgatcagcct tcaacgccat
    21241 gttgcgattt cagaattgct tgcagacgtg aaatgcgaag gggtcgtgta cgtcacagca
    21301 ttcccgaatc gcgagaagcc ttttacctcg ttcctgccag aaattgcctg ggagaccgag
    21361 gtctgggtcg cgagtgatcc cgatcacctc attcatttca atggagagag attcctgggc
    21421 ccctatcccg ttcggcaacc cgactagctt cccgcgaatc ggacggcgcg accgtctaca
    21481 gcaacatgtt gtgcgggaca gacggtccgt caataagccg gtgcgagccc ttcgatcatc
    21541 gggaagtgcc gaacgttcgt agtcaggaga tcggcgtcgg cgagcatcga ggtcgccgcg
    21601 atcaggtagt ccgccatgtc aatcccgcta tgtgacggtc ggaattttcg ggccagatca
    21661 ccagccaggc ggcagatcga ctctccaacg gggacccatt cgatcgcggt gaaaaagcgc
    21721 tcgagagacg cttcttcgcc agccctcacg ccggccagaa gctcgaatct ggtcacctcg
    21781 cttgccagca gtggttcatg tccctcggcg aggccggtca acaggcggat cgctgctccc
    21841 ttgcctcgga gaaagtcgat cacgacgctg gtgtcgagca gcttcattcg atgccaaagc
    21901 tggcaaggcg ttcatcaagc ccgccgggac ggacttcgtc cacgaagtca gaacccgacc
    21961 cttcgctctt ccatgctccg gcagtctcga gaacgatccg cagcgggtcc gagtgaccac
    22021 gaccgtagga ccgacgcacg gctctccgga tcagctctga acgggtggcg ccggtgtccg
    22081 cggctgcccg gtcaagcatt tcgagctcct cactgccgag atagacctgt gtccgaccca
    22141 tgacaccact atacacctgt atacaccaca cacagcagcg ccgtcgcaac gaagattcct
    22201 gtcgggactg ttaccaggcc gattcttgtg aactcgcgcc aggagacggc ggcgccgtct
    22261 tcgatcatca cacggcgcca gagcagattg gcgatcgaac ccggataggc gaggttgggt
    22321 ccgatgccga ccccgatcag catcgccagc agcgcccctt cgccggccgg agcgacagcc
    22381 ggtagcagga tcagggtcgc cggaatgttg ttgatcagat tggccagtac ggcggcgccg
    22441 aaggcgatca gaagcagcga gccaaagccg gtcccggcgg gaaccaggtc ggcaatcagg
    22501 tcggtcaggc cgaggtcgcc cagcaaccag gcgagtccgc cgagcgccag cagcgcggcc
    22561 agaaagagcg gccggatcgc tctgaccagc tcaaccggac cggcggtgcg tcccgccagc
    22621 tcgccaagaa ccatgaccgc ggcgccggcc caggcgatcg ccaggggctc gaccccgagc
    22681 ggaccgctga cgatgaaccc ggaaagcacg attccgagta gcgccgccgg ccagacactt
    22741 acctcgggtt tgacagtcgc cagctcgggt ggtgaccgga cttccgcggg ccgggccagc
    22801 cgcgggacca gcaaccgctc gacggcgatc gcgcccagcc agggcagagc catgagcgcg
    22861 gtgaaatgcc caaaggagag gtcggtctcg tggaagacca tgatgttggt caggttggaa
    22921 accggcatca gcagcgaagc tgaattggcc aggtgcaccg tggagatcga gaaggcttca
    22981 tgaccgatgc cgcgcctcga tgcaaccgca agcactaccg gggtcatgac cagcaccgcc
    23041 gcgtccaggt tgagagccgc ggtagccgcc gccatggtcg cgaagagact gaacagcagg
    23101 ccgcgctcgc cagatcccgc gtgggccacc cgccaaccgg cgtagtcgaa tactcccgcc
    23161 cgatgacagc cgttggtcag gaggagcagc gcggccagga aggctgctgt gtaggtgatc
    23221 gcctcgatct atgaagtctg accaaagacc tcgatgatct tttcgttgta ctcggtgatc
    23281 tgctcgtttg gaatgaagta ggtcggggcg cccggcagga tcgcgtcgcc gaactgggcg
    23341 acctcggcca ccttttcctt cactttgtcc ggggtgccaa ccgcgcaata ggtctcgacc
    23401 atctcgtccg aaatcgcttc ggccactccg gcgaggtcgc ccatgcggaa ggccgcaccg
    23461 gcatcggcgg cgttctgacc gaagtcgtgg agttcgaaga gcggcagata ggtcttgact
    23521 gtcgcgtaga aagccagggt cttgcgggca gcttcgagac cgcgttgata gtcgtcgtcg
    23581 attgccacgc agatgctcgg caggaactcg aaggaagaac gatccctccc cgagcgggaa
    23641 agaccgagtt cgaaggccgg gacgatctcc tgctcgatcc atcgggccga gcagatgatg
    23701 tggccgatca gaccgtcggc gacgtcaccg gccatgcgcg acatgccctt ctgtaccgcc
    23761 gccgtgtaga tcgggatgtc ggcgcggacg ggcggggtcg atcggaacca gcccttgatg
    23821 tcgaggtcgt agtactcgcc cttgtaggca accggctcac tggccgctgc ggcgtggatg
    23881 atcgcccgga ctgattcgat cgtttcccgt agatgcgggg caggccggcc gtactcggcg
    23941 ttgtgccagg tctcgttcag ccgtttgacg cccgggccga gccccagccg gaagcgcccg
    24001 ccggtgatct catcgatgtc catcgcactc atcgccatgg tcatcgggct gcgcacgaac
    24061 ccccaggcga tcgcggtccc gatgcccatc gattcggttt tcgcaccgag gaatgtcgtc
    24121 tgggtgaatg cattgcggaa tagttcgatc gcccagccgc agtcgaaacc ggccgcttca
    24181 gccagccggg ccatggccgc catgtcctcc ggcgtctccc ccgtgatccc gatcgaaacc
    24241 ttctccatag cgctcatgct cctgagcgta tctgtttgaa atcccgacca ggtggtagtg
    24301 ggtcattgcg tacccgtccg tacgcaagtg gagagaaacc caggcaccaa gggaaaggca
    24361 agcatgaaca ggatgttgat gaaactggca ggactggccg tcatggcggt cgcttttttc
    24421 ggctcgaccg ctgccgcgtc ggcagatccg gtcgttttca atttcgatga atcgatcatc
    24481 aatctcggag gcggctcggg cccgcaaggc ctcaagctga tcgaccccga tgcggacccc
    24541 gccgatgccc cggccaccct gaatggcgag gtggacacgg caactgacgc tttcaccgcc
    24601 gcagcatccg gcttcacctt tccccagaag accacccagg tttcaggtca ggacgtcgtc
    24661 gtgacgatca ccgccaacgc ggcgatcacc ggaacgtttg acaccgacac cggcaccgcg
    24721 gacataacgg tcccggtcac cgtcagcgct gccgtactcg gcaatacctg cacgacctcc
    24781 ttcccgctcg aactcaagac cacgggcact ctggtcgaac ccggcggtaa ccatgacgcg
    24841 gccccctttg accccgacac cggtgagggg agcgtctaca gctcgttcac ggtgccgaac
    24901 tcgaccggtg gcgccctctg cggcgtggtc gacggccaga tcggcggcgc cggtgacatc
    24961 tggctctccg gcactggcgg aatcgatgaa ggcatcgtca ccgtctatcc cggcgatgac
    25021 ggcaccagcc tctccaccac cgacggcggc tgggtccaca gcacctcgta cggcccgctc
    25081 tgcgcactca tcccgggagt tctctgcccc gcgatgaacg ggaccttcga atcagccaac
    25141 ggcagctccg gggccgccga cggcttcctg cgcgacacgg ccaccggcct gacagtcacc
    25201 ccgaccggcc tcggccagac ctcgaccgga gtctggacca gccccgagtt cacctatgac
    25261 ggggccgaag gcgaaccggc cgagactctg agttttgccc tggccaggaa aggcaccctc
    25321 tccgacctgc tcagtcagtc cagcgccaac tggcaggtcg acctggtcaa gtccagcgac
    25381 agttcgatca tcccgctgat tccgaacggt cccgcaacca atgccgattc ctgggtgaac
    25441 atcccgggca gcgcgctggc ggctgacggc ctgacgattg gcgacgcgta tcgcatcgtg
    25501 atcaccacca acgtgatcac cgagacgctc ggcctggccc cctctggaac cttcgactac
    25561 gacgaggtgg cgctcacggc ccgtcgcgca accccgaccg gtccgacggg ccccactggc
    25621 ccgaccgaac ccacaggtcc tactggcccg acggaaccga ccggtcctac cggccccacc
    25681 gagccaaccg gcccgaccgg tccgacttcc ccgaccggcc ccaccggccc cacgggaccg
    25741 accggaccga acaagccggt caccctgacc aagggcaacg ccgccttcaa cggcaagcga
    25801 ctgttcattc gcctgtattg cccacgcgag ttcaagccga agtgcaacac ggtttcggtg
    25861 ccggtgacca ggaaggtcgc aaataaggcc ccgaagaaga tcaagcgcct gtcgaagccg
    25921 atggccaggc cgatccgggt gacggtcaag tcgggcaagt ggaagaaggt ctcctacctg
    25981 atcaagcctg cctaccggaa caaggtcagg aagatggccg gcaagaacaa acggctgctg
    26041 gcgatccgcg actacatcaa ggcgaagaag ttcgccggcc ggaagttcaa gggcaagacc
    26101 cgaacggtct tccacagcca caaggtcgtg atcggcaagg ccagataacg aacctccctc
    26161 ccaaatgaag tgccctccaa atgaaagggc ccgggtctct ggacaagacc cgggcccttt
    26221 cgccttctga cggactcaga ttcgttcgat gagcgtggcg gttcctatcc cgccgccgca
    26281 gcacatggtg accagaccgg tcttcttgtc ctgacgctcg agctcatgga gcagggtggt
    26341 catcaggcgg gcgccggtcg agccgagcgg gtggccgagg gcgatcgcgc cgccgttgac
    26401 atttaccttg tccatgtcg
//
LOCUS       DAOQKN010000012        25030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_818_length_25030_cov_6.355716, whole genome shotgun sequence.
ACCESSION   DAOQKN010000012 DAOQKN010000000
VERSION     DAOQKN010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 25030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25030
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_818_length_25030_cov_6.355716"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="PLJ64_01700"
     CDS             complement(<1..392)
                     /locus_tag="PLJ64_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92239.1"
                     /translation="MPLSEDSRALLQLLLGRGKNYADISGLLGIDEAEVRSRAGTALA
                     ELDDSNRPPDPELTDYLLGQADPIGRADVGNRISSDPALAERAESITDQLRLLVPGAE
                     LPRPGSAEGGKPKPATKPPAPVAAAAPET"
     gene            499..927
                     /locus_tag="PLJ64_01705"
     CDS             499..927
                     /locus_tag="PLJ64_01705"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNC92240.1"
                     /translation="MPDVTLPESPFDNLLGTEWVSSDPDQAIVRLTMRDDHRQPMAIM
                     HGGVMASLVESVCSMATARAVLPEGRIAMGQNISVNLLRPISSGEVEVVATAVHRGRM
                     TWVWRAEVKDDEGRVCAISQMTMAVREAPEGADLSQFVKK"
     gene            complement(1014..2633)
                     /locus_tag="PLJ64_01710"
     CDS             complement(1014..2633)
                     /locus_tag="PLJ64_01710"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HNC92241.1"
                     /translation="MSRIPKIALAQVNPTVGDLDENAALVCEWIELAGRAGAGIVVFP
                     ELVLTGYPAEDLYLRADFVAAAGRRLEEIAARVGDITAVVGFPDPVGNPVGRAIAANA
                     AAVIRNGEIRGVYRKRLLPNYGVFDEYRTFRNGTDPLVLESAGTQLGVTVCEDCWDPE
                     SPVFEELAPGAGLIVNSSASPYHRGKGRQREEIFRRVATAKGLPVAMVNMVGGQDELI
                     FDGSSALIAPDGSILARAGQFTEDLLIYDPAESPAPVADWMDDLDEVYTALVTGLRDY
                     VVKNGFRHVGLGLSGGIDSALVAALAADALGPEQVTCVVMPSPHSSGATQADAREMAN
                     RLGTGLIEFSIEDAMAAYDRTLGEPAAGITAENLQARIRGNLMMALSNSRGWLILTTA
                     NKSETSVGYSTLYGDMAGGLAPIKDVPKTLVYDLCRHRNGSSPVIPESIISRPPSAEL
                     RPGQLDSDSLPDYDELDRILKMYVEDDLGPAEIADRGEDPAVVADVIAKVDRAEYKRR
                     QAPPGLKITTKGFGRDRRMPITNRYRPWQPS"
     gene            2833..3765
                     /locus_tag="PLJ64_01715"
     CDS             2833..3765
                     /locus_tag="PLJ64_01715"
                     /inference="COORDINATES: protein motif:HMM:NF015298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNC92242.1"
                     /translation="MSTSRIFRASMSLFTCLALFMAMTSGSSAANSQGTGGKTASESP
                     APTGPVKLKVADYSVNFGGRFSLRGKTGAARKGHVRIQSRVNGRWHNLRKAGTDGMGR
                     YRLKLRARHNLVIRAVAGDGRVSKTRRVTVIGHIRLDRSERYVKLGRKLRVSGVVVPR
                     GVRTVKVRVRGEGVMRTRSRANGRFAFRWTPRRNGDFSYRVWARKSRKSTGDSSRPHR
                     FSALRPGEASYYGPGLYGNGVACGGTLTPTTRGVANKYLPCGTRVTLQYGNRTVVVRV
                     IDRGPYGAGRDWDLTEQTKKDLGFGGVGTVWTNK"
     gene            3881..4228
                     /locus_tag="PLJ64_01720"
     CDS             3881..4228
                     /locus_tag="PLJ64_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92243.1"
                     /translation="MSSAESKNLEAISKAIDHHNATCPFPAAEVRMNPFEVERLGWEE
                     IRGLPIVPDPNIGTGRFHIVCSRDMEGDELEEVEAIAEELVTVTPGKRDTPAEPGQEP
                     GEPGIPDNPGRWN"
     gene            4313..5866
                     /locus_tag="PLJ64_01725"
     CDS             4313..5866
                     /locus_tag="PLJ64_01725"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNC92244.1"
                     /translation="MLLSAAIWTSSAEAGRIAYVADYDANVVSQLDTETNAFLSTSFS
                     GMTDTYMLQVSPDGKYLWIPRFTPNKVSVVDLTDGQALPDIDVDQNPVGVVFSPDSSM
                     AYVLSYDASTVQVFDTATRQQIGDDIPVGAAPYYESLSPDGSRLYVANFDDDTISVIN
                     TADRQVVGDPVPVCADLYSMAISPDGTRLFIGCAGSGDMVMQLSTDTMEATSFSATLP
                     EDPEYLTISPDGATGWAVIRTLDEVVSFSTATGAAIGDPIPVGHEPYHLAITPDGSKA
                     FTSNYEGTQGITPINIATRVAGTLIPDTGGPWQMAITPDQPPSALIAAPISIVAGQPA
                     KFDAVNSIDPDGTISRYDWNFGDGKSKPDGKVTESHVFTKAGQFSVSLILTDNEGCST
                     GFVTAGYTAYCNGGAGASTTLNVRVKAPSNNFSFGRIKLNRKRGTASLQVRLPGPGVV
                     MLAGKNVRGARKTVKKAGKSALSVRITGKAKRALSGRGVVKVKAKVTYRPKYGKPRTK
                     TRTVTLRRN"
     gene            complement(5874..6668)
                     /locus_tag="PLJ64_01730"
     CDS             complement(5874..6668)
                     /locus_tag="PLJ64_01730"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNC92245.1"
                     /translation="MPFAELNSAGLVSPVLAAVDSVAGVGLVTAFIAGFVSFLSPCVL
                     PIVPGYLSAVSGVSVNELDDAGWKKVLTPSLIFVGSFSLIFILLGMGATRIGSLLSDN
                     QELLEKISGALIIALGVLFVASFFVTRLNREWHVDALMQRAGKGGPLVAGAAFAFAWT
                     PCIGPTLGAILSAASTTDSTAKGALLLAVYSAGLAIPFLLTALAFSKMTSAFAVIKRH
                     YGVVIMVGGLVLIAMGILIWTGELSRINIEIQQWSQETGLDFWNSV"
     gene            6695..7558
                     /locus_tag="PLJ64_01735"
     CDS             6695..7558
                     /locus_tag="PLJ64_01735"
                     /inference="COORDINATES: protein motif:HMM:NF005878.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC92246.1"
                     /translation="MEHSLEGAVVVVTGGAAGIGRATATKMAESGANVAIGDIHAEKA
                     AETAEEITSRVSAGGSGSGGGAIGRPLDVTDRDGFEIFLDGVEAEFGPIDCLVNNAGV
                     MLLGPVDQESPAATKAMIDVNLLGVINGTQAAMDRMKPRARGRIVNIASQAGKAGMAG
                     GATYCATKFGVIGFSEAVRAELKGTGVSISWVLPGLVNTELSAGLPDIPLMDLLSPDD
                     IADAVLKALTRGGSEIWVPARNQWLDAPARLLPRPMREKVFAMTGADKVLAEADPDTR
                     QDYEDRHSGVS"
     gene            7713..12305
                     /gene="gltB"
                     /locus_tag="PLJ64_01740"
     CDS             7713..12305
                     /gene="gltB"
                     /locus_tag="PLJ64_01740"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HNC92247.1"
                     /translation="MTEFAPSSATRDGLYDSTNEHDACGVAMVARLDNEPRHEVVSQG
                     LLALENLEHRGAAGADASTGDGAGILIQLPDLYYREEAGVDLPPAGQYGVAMCFLPRE
                     NEERREELKTLITETTEAEGQVVLGWRDVPVDPGHVGNTANDSRPHVAQLFIGAGESH
                     RSDQDAFERKLYVIRRVCEIAAGEELYIASCSSRTIVYKGMLTSYQVPGFYPELQDER
                     VRSAMALVHSRFSTNTFPSWDLAHPFRLIAHNGEINTLRGNINWMRARESQLASELFG
                     DDLKKVTPVVRPDGSDSATFDNVLELLMLAGRSLPHAAMMMIPESYQGRTDLSDELKG
                     FYAFHSCLMEPWDGPAAVCFTDGRVIGATLDRNGLRPGRWVETNDGMVVLGSEIGLLD
                     IAPEDIKRLGRLQPGKLFLVDLEQKRIIEDEEVKREVATQQPYGEWFENASVHFDDLP
                     TAYVTMTGIQPTPRRQQAFGYTQEDLRVLITPMAATGAEPIGSMGNDVALPVLSDNRP
                     PLYSYFKQLFAQVTNPPIDPIREEIVMSLSAGVGAERNLLDESEQHAHQLCLDQPILM
                     NQELETIRHVNHDIFRADTIDITWDVAEGTAGIESRLDAICEEAEEAIEKGFNILILS
                     DRRTGPRRVPIPALLAVGAVHHHLVRAGTRLRAGLILESGEPREVHHMATLIGYGCSA
                     INPYLMLDSVDDLVVNGLVPDMQSPEKAQRNVVKAIGKGLLKTISKMGISTTQSYGGA
                     QIFEAVGIEKEVIDKYFTGTASRIGGIGLDVIAQETMDRHASAFPGAGDDLLPVGGVY
                     AWRRDGEFHQWNPETVSLVQHAVRGEGGSAQEKYDEFAQQVNEIALKRATLRGLFKLK
                     AKDSGPVPIEEVEPAKEIVKRFATGAMSLGSISRESHENLAIAMNRLGGKSNTGEGGE
                     DPVRFTPEENGDSRRSAIKQVASGRFGVTFHYLANADQLQIKMAQGAKPGEGGQLPGH
                     KVDRYIGSVRHTTPGVSLISPPPHHDIYSIEDLKQLIYDLCCSNPKASVSVKLVSEVG
                     VGTVAAGVAKANADHVLIAGHDGGTGASPLSSVQSAGIPWEIGLAETQQTLIRNDLRS
                     RITVQTDGQLKTGRDVVVAAILGADEMGFSTAPLIASGCIMMRACHLNTCPVGIATQD
                     PELRKRFKGQPEHVVNYFFFVAEEARKIMAGMGVRSIDELIGRIELLETDDAVDHWKA
                     RGIDLSGLLAWPEGIDHSVDRRRTRPQEPVLDDNLDWKILEQAGDAVENGIPVEFDMT
                     VQNVDRCVGGITSNAIANAQGAEGLPEDTIKVNFRGAAGQSFGGWLAPGITFSLRGEV
                     NDYCGKGLSGGILAVSPPDGAAYQPEENVIVGNTSLYGATKGRAFFRGIGGERFAVRN
                     SGATAVIEGVGDHGCEYMTGGTVVVLGPTGRNFAAGMSGGIAYVLDEQDEFSGRVNPS
                     MSNQLEEPDEKDRIELRGLIQEHLERTGSEVARRILDDWESTSARFVKVFPEDYKRVL
                     AELAAEGENEPGVGPETNDVEVIGEPATGVASEKGA"
     gene            12309..13898
                     /locus_tag="PLJ64_01745"
     CDS             12309..13898
                     /locus_tag="PLJ64_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HNC92248.1"
                     /translation="MGKLGGFLQIERAGIPYRDPAERVGDYNEFVVRRSDEELADQGA
                     RCMECGVPFCHNGCPLGNLIPDWNDLVYHGRWQDAIRQLHSTNNFPEFTGRLCPAPCE
                     AACVLEIREGEAVTIKQIEQSIADRAWKEGWIRPRPPKRETGRHVAVVGSGPAGMAAA
                     QQLRRGGHSVTLFERDEAAGGLVRFGVPDFKIEKTVVERRVQQLIHEGVEVRCGVEVG
                     VDVTVEELKERFDAVVLATGSRVPRDLPAPGRENDGVHFAMDYLYSRNRWLASEVGPK
                     PMTEAPAPAPISAKGKHVVVIGGGDTGADCVGHSIREGAAEVVQLELLPEPPEHRPDD
                     RTPWPLWPTKFRLSYAMEEAKGIEKGEQDFSVTTTSFNGEDGQVRSITVAKADPNPPF
                     AAVEGSEFEIKADLVLLAMGFLHPEQELLDGLGVDKDQRGNVKAGTYETSVPGVFAAG
                     DARRGQSLIVWAINEGRQAARMVDRFLGTLPSPSPVPRTPDVPTGNVNPADEGPEGPP
                     KHVAPGLGPDPSASAPGFAQE"
     gene            complement(13970..14746)
                     /locus_tag="PLJ64_01750"
     CDS             complement(13970..14746)
                     /locus_tag="PLJ64_01750"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HNC92249.1"
                     /translation="MNTSSHADSLPVLAKPKIRSGRPSPWKKRGLITVAALGTIALLV
                     VLATNLIVLLDSDGTVWNDPADAKHAEVAIVPGAMVNQDGTMSMMLADRVNQAAALYR
                     DGKVEKILVSGDHGQWTYDEPTTMRKALVAQGIPANVIYEDHAGFNTNATMQRARDIF
                     GVSDATVVTQGFHMKRSLFLAQAAGLDANGLTSDLHGYGGQGIKSDIREVLSRTKAVF
                     DLLRGAPVTGGEPVPITGPARASWGPEAPSGTPPAGSPSD"
     gene            14861..15793
                     /locus_tag="PLJ64_01755"
     CDS             14861..15793
                     /locus_tag="PLJ64_01755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNC92250.1"
                     /translation="MAHEHQHGDHGHSHGPGAWRDADRKALLIAAGLTAGFMVAEVVG
                     GLLTDSLALLADAGHMLSDTFSLFLALGAVALAARPVTARRTFGFKRAEILAALINGV
                     LLVVVSVWIVIEAIDRIGNPPEVLGGWMLVVAVAGLAVNLIAAWVLSRSAGESLNVKA
                     ALRHVLADVAGSVGVILAALIILTTGWELADPIVSIVISVLIAASAWSILRDSVDVLL
                     EAAPPGLDTEEIGYSMAALPGVDQVHDLHIWQITSGMPMLSAHVLVGPDSDCHAIRGD
                     LELLLRDEYEIDHTTLQVEHSASQEPITIEGFNP"
     gene            15838..16578
                     /locus_tag="PLJ64_01760"
     CDS             15838..16578
                     /locus_tag="PLJ64_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HNC92251.1"
                     /translation="MNWFDRRLAAFDIETTGTDIENDRIVTAAVSIVGGGEQTVSRNW
                     LVNPGVPIPAEATEVHKITNQMAMRDGVPTATAVEEITTLLAGALEEEVPVIAFNARF
                     DLTILDREARRHGVEPLVDRVGGPDGLLVVDPYVLDRHYNKFRKGRKNLTILCEAFGV
                     PLTEAHAADADALAAVRLAWRMASRNEELGQMELFELHESQIHWAAEQARDLQEFFAK
                     NGRDEHVEGAWPVVPAAAPAVFEDRLAA"
     gene            16630..17307
                     /locus_tag="PLJ64_01765"
     CDS             16630..17307
                     /locus_tag="PLJ64_01765"
                     /inference="COORDINATES: protein motif:HMM:NF022805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3159 domain-containing protein"
                     /protein_id="HNC92252.1"
                     /translation="MSEVHRTEDQVEVELDQKEEINLLDELGGPQGIADSSIPGLVFV
                     IAYTVSGQKLELAAGVAVGIALLIALIRLVRGERARYAASGFFGVALAAFIATRTGKA
                     EDFFLPGLLFNAGYSLAYAISIAIRWPLMGVFIGPLIGEGMTWREDPERVKLFNKISW
                     LWVGTFLLRLAVQLPLYLSGALVALGIAKTAMGLPIFALAIYLSYLMLKAAGIDLQQK
                     KEAEEAG"
     gene            17362..19605
                     /locus_tag="PLJ64_01770"
     CDS             17362..19605
                     /locus_tag="PLJ64_01770"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNC92253.1"
                     /translation="MSPSSSPTRTGEAPGRAGANGDANPRPKRKDVPIRLRVGASEGL
                     YRIRPFEMVVRTHPPERPGEYRDVSELWTLDKDEFGSRGRIDTTMGVGMAAAAWAPLR
                     SLLEADVPDRLELSDAEVLDLLDGAAERLEASGIVLDWDPDLDQSLTGKVVVGSSGRL
                     SQSVPDVFDQNALQGFSWQLSLGGTPLSEEEMAVIADTDGPIARLREKWVLVPRVMAE
                     RARHPDLKPLTAIDALSVALTGSTVVDGQRVDVEAKGWVAQLREQVTGDSEQSLIEPP
                     ADLNATLREYQVEGMRWLHRMTSLGLGGCLADDMGLGKTVMLISLHLHRRLNPQTAGP
                     TLVVCPASLLGNWEREIQRFAPTEKVRRYHGPNRSLDQITSGFVLTTYGTMRLDAELF
                     EAVPWGLLVADEAQHVKNPRSAGAKALRTIPARSRVALTGTPVENNLSELWAILDWTT
                     PGLLGSLGAFRKKWADPIEVEGSQSATRLLNRLVRPFLLRRRKSDPGIAPELPPKTIT
                     DQPVELSPEQIKLYEAAVRENMLQIRTAEGINRRGLVLKLLTSLKQICNHPAQFLGEE
                     GGPLAERSGKLELLDELLDTILSEDSSVLVFTQYVAMARLLSRHLEERGIGAMLLHGG
                     TAVRQRERMVERFQDGEVPVFLLSLKAAGTGLNLTRADHVIHYDRWWNPAVEEQATDR
                     AHRIGQLNSVQVHRLIAEGTIEDRIASIHETKRELADAIVSTGEAALTELSNEDLAEL
                     VELRNAG"
     gene            19605..20243
                     /locus_tag="PLJ64_01775"
     CDS             19605..20243
                     /locus_tag="PLJ64_01775"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC92254.1"
                     /translation="MGGQAEGSRTWRGQTIEERQAGRRERLIEAGIELFGTRGYANTP
                     VKAICEEAGLTERYFYEAFEDRESLLDEIYSMVLADCRRATVEAIDGAPDDFEASMRA
                     GLTAFAHEWIRDPRRARIQEIEVVGVSPEIERKRRDSIHAFAGIVVDRTLQLGGREQL
                     GALRLDVIALGLIGSVNEQLIDFVLGDLQISLDDLIENQIAAFAAVASQLLE"
     gene            20293..21123
                     /locus_tag="PLJ64_01780"
     CDS             20293..21123
                     /locus_tag="PLJ64_01780"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC92255.1"
                     /translation="MSEQQVGPKTIVITGAARGIGLATARILATRGNRVVMSDLDGEL
                     AAAEAEKIGDRAAGFGVDVTDRDAVREHLEMVEREFGPVDVMINNAGIASASPNILEQ
                     DPKITERTIDINLKGAMNGTIEAIRLMEPRRRGQVVNVASLAGIIGVKGLAAYSASKF
                     GLVGFTESIRLEFADKGIKLTCVMPGPVDTGMMDGTSSSPLVSMLAPEEMATGIADAV
                     TTGKPRVSLPRSSWLLARTISVLPPAIGIRVGHWTRIDRIYTEIDPAARAAYEERISR
                     "
     gene            21140..22456
                     /locus_tag="PLJ64_01785"
     CDS             21140..22456
                     /locus_tag="PLJ64_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNC92256.1"
                     /translation="MDRDPGGTRLSGRERTSNGRPSAETVEAIWRIESARLVARLSRL
                     TGEVGLAEDMAQEAFVAALEVWPGSGIPRNPAAWLMTTARNRGIDRIRRDRVLTEKYG
                     QIAHEMELAGPGPGPEQAVLNEDRIGDDVLTLLFTACHPCLTTETQVALTLKAVGGLT
                     TDEIARAFLVSDSAIAQRIVRAKRTLADSGIPFEPPSTDEMGERLAAVLGVIYLIFNE
                     GYSATAGDQWMRTELSVEALRLGRILVGLAPREPEVHGLVSLMEIQASRFPARTGPQG
                     EPVLLMDQDRSRWDRLLIRRGLKELETARSLGGNGPYVIQASIAACHARALRPEQTDW
                     QEILGYYDELAALGDSPVAELNRAVAMAMSGDPRSALDAVDRVATDSRMKGYYLLPSV
                     RGDLLERLGRSGEAAAEFERAASLTDNQAERRVLIERAERAKDFPG"
     gene            22532..22960
                     /locus_tag="PLJ64_01790"
     CDS             22532..22960
                     /locus_tag="PLJ64_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNC92257.1"
                     /translation="MRCMVIVKATEDSEADVMPSEKDLGDMGSFNEELVNAGVMLDGD
                     GLKSSRHGVRITYSGDEAQVTDGPFSETKELIAGYWVWEVKSLEEAIEWARRIPFHHG
                     ESVEIRPFHELEDFGEAVTDEFRERQARMEATIEEQKRDA"
     gene            complement(23013..24263)
                     /locus_tag="PLJ64_01795"
     CDS             complement(23013..24263)
                     /locus_tag="PLJ64_01795"
                     /inference="COORDINATES: protein motif:HMM:NF015541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HNC92258.1"
                     /translation="MTFELTRLRAVVLALAMLLTTALLIEVAQPAGADAAAKKTKKKK
                     GPKQGPAGLKFYSPPKKLPTAHGKLIWQRKAGGSVPLKSAASTKLVLYTSRSTTGKQI
                     AVSGSVSIPKGKAPKGGWPIISYAHGTTGIADKCAPSRNTKGGVADPYISYMDPLQNA
                     WLRAGYAVARTDYQGLGVPGVNHPFLVGVPAGRSVLDIVSASRQLGLKIGNRYLIAGH
                     SQGGHAALFAAGLASSYSKDIKLRGTVSYAPASHFKEQASFLPGLTSPNGLTALATLL
                     VRGMSISYPQLNANEALSDTILPFYPQTLTKCLSELAGSDNLGGIAPSELIRSGYDMT
                     PLNGILEAQNPSVTTSAPILLAQGTADSTTFPFLTDQLDTELVGKGDTVDYKKYEGVD
                     HGGVVAAAQNDVMSFFESRLPSGK"
     gene            24381..>25030
                     /locus_tag="PLJ64_01800"
     CDS             24381..>25030
                     /locus_tag="PLJ64_01800"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNC92259.1"
                     /translation="MTDILSRRDLQFQLNEWLDVERLTERDRYRDQTLDDWNDVLTLA
                     EQIAEDQFQPHNRKADLNEPAIGEDGKVVLIPEVAQALETLGETGLMAAAMPEEVGGM
                     SLPATVSSAIFAWLHAANVGTTAYPMLTAGAANLLIANASEEQVETWVKPMVEGRFHG
                     TMCLSETQAGSSLSDITTKAVPQDDGTYRIFGNKMWISGGSHELGDNIVHLVLAKIP"
BASE COUNT         4534 a         8149 c         8299 g         4048 t
ORIGIN      
        1 gtctctggcg ccgcggcagc gaccggtgcg gggggctttg ttgccggctt cggcttgcca
       61 ccttcggccg atcccggacg cggcagttcg gccccgggga cgagcaacct gagctgatcg
      121 gtgatcgact ccgcccgttc ggcaagggcg gggtccgatg aaatcctgtt gccgacgtcg
      181 gcccggccga tcggatcggc ctgaccgagc agatagtcgg tgagttccgg gtccgggggc
      241 cggttcgagt catccagttc ggccagcgcg gtgccggcgc ggctgcgcac ctcggcctcg
      301 tcgatcccca gcaggcccga aatgtcggcg taattcttcc ctcggccaag cagcagctgc
      361 agcagcgccc ggctgtcctc actcaacggc atgacggtga accctacggg ttcggcgcgg
      421 ttggctgttt cctgagtgga ccctgtgaag aagccgggtt caccgggcct ccggttcgga
      481 cccgtatgat cccggccgat gcccgacgta acgcttcccg aatccccgtt cgacaacctg
      541 ctcggcaccg agtgggtttc ctccgaccct gatcaggcca ttgtccgcct gaccatgcgt
      601 gacgaccatc ggcagccgat ggcgatcatg cacggcgggg tcatggccag cctggtcgag
      661 agcgtctgct cgatggccac ggcccgggcc gtgctgcccg agggaaggat cgccatgggg
      721 cagaacatct cggtcaacct gctgcgaccg atctcgagcg gtgaggtcga ggtagtcgcc
      781 accgcggttc accggggccg catgacctgg gtctggcggg ccgaagtcaa ggacgacgag
      841 gggcgtgtct gcgcgatctc acagatgacc atggccgtcc gcgaagcccc cgaaggcgca
      901 gacctttcgc agtttgtgaa gaagtaactc agtcagagcc cgcccatcca ttgaatgtct
      961 cagtgggcgg gcgggtttga ctgggagggc gggtcggacc ccgcccgact cgcttatgag
     1021 ggctgccacg gccggtaacg gttcgttatc ggcatgcggc ggtcgcggcc gaagcccttg
     1081 gtggtgatct tcagtccagg tggagcctgg cggcgcttgt actcggcccg gtcgaccttg
     1141 gcgatcacgt cggcgacgac tgccgggtcc tcgccccggt cggcaatctc ggccggaccg
     1201 aggtcatcct cgacgtacat cttcaggatc cggtcaagct cgtcgtagtc aggcagcgag
     1261 tcggaatcga gctggcccgg cctgagttcg gccgacgggg gccgggagat gatcgattcc
     1321 gggatgaccg gggaagaccc gttgcggtgc cggcagaggt cgtagaccag ggtcttcggc
     1381 acgtccttga tcggtgccag cccgccggcc atgtccccgt agagggtcga gtatccgacc
     1441 gaggtttcgc tcttgttggc cgtggtcagg atcagccagc cccggctgtt cgaaagggcc
     1501 atcatcaggt tgccccggat ccgggcctgc aggttctcgg cggtgatccc ggccgcgggt
     1561 tcgcccaggg tgcggtcata ggcagccatc gcgtcctcga tcgagaactc gatcagtccc
     1621 gtcccgagcc ggttcgccat ctcccgggca tcggcctggg tcgcgccgct ggaatggggc
     1681 gagggcatta caacgcaggt gacctgctcg ggccccagcg catccgccgc cagggcggcc
     1741 acgagggcgg agtcgatgcc gccggaaagt ccgaggccga cgtgccggaa gccgttcttg
     1801 accacgtagt ccctgagtcc ggtgacgagc gccgtgtaga cctcgtcgag gtcatccatc
     1861 cagtcagcga ccggcgccgg tgattcggcc gggtcgtaga tcagcagatc ctcggtgaac
     1921 tgaccggccc gggcgaggat cgaaccgtcc ggcgcgatca gggcgctgga cccgtcaaag
     1981 atcagctcgt cctggccacc gaccatgttc accatcgcta ccggcaggcc cttcgcggtg
     2041 gctacccggc ggaagatttc ctcccgttgc cggcccttgc cccggtggta gggagaagcg
     2101 gacgagttca cgatcagccc ggccccggga gcgagttctt cgaagaccgg tgactccggg
     2161 tcccagcagt cctcgcagac ggtcacgcca agctgtgtgc cggccgattc gagcacgagg
     2221 ggatcggttc cgttgcggaa ggtgcggtac tcgtcgaaga cgccgtagtt gggcaaaagg
     2281 cgcttgcgat agacgccccg gatctcgccg tttctgatca ccgcggcggc attggcggcg
     2341 atcgcccggc caaccgggtt cccgaccggg tcggggaatc cgaccacggc cgtgatgtca
     2401 ccgacccggg cagcgatctc ctccagccga cggccggctg cggccacgaa atccgccctc
     2461 aggtagagat cctcggccgg gtaaccggta agcaccagtt cggggaagac cacgatcccg
     2521 gctccggccc gcccggcaag ctcgatccac tcgcagacca gggcggcgtt ttcgtccaga
     2581 tccccgaccg tggggttgac ctgggcaagg gcgatcttcg gaatccggct cactggctga
     2641 gtatcccgat ttgcaggggt tcctgccgtg tgaagcaccg cacacacagc cgcaaacggg
     2701 atctccagac tcctttgctc acgaaacgct aacaatcggc gagtcctgac gaacctgcag
     2761 acagacggcc cacgaaaggc cgcccgcgtc cgtcagccag ccccaacggg cgccgagaag
     2821 gagaagcctc aaatgagtac cagccggatt ttccgtgctt cgatgtccct gtttacctgc
     2881 ctggccctgt tcatggcaat gacctccggc agcagcgccg ccaacagcca gggcaccggc
     2941 ggcaagacgg ccagcgagtc gcccgccccg accggcccgg tcaaactgaa agtggctgac
     3001 tacagcgtca acttcggcgg caggttcagc ctgcgcggca agacgggcgc cgcccgcaag
     3061 ggtcacgtcc ggatccagtc gcgggtcaac ggacgctggc acaacctgcg caaggccgga
     3121 accgacggca tgggccgcta ccggttgaag ctgcgtgccc gccacaacct ggtgatccgt
     3181 gccgtggcgg gtgatggccg ggtttcgaag acccgtcgcg tcacggtcat cggccacatc
     3241 aggctcgacc gctccgagcg ctacgtgaag ctcggccgga agctccgggt cagcggcgtg
     3301 gtcgtgccac gcggagttcg cacggtcaag gttcgcgttc gcggtgaagg tgtgatgagg
     3361 accaggagcc gggccaacgg tcgtttcgcc ttccgctgga ctccgcgccg aaacggcgac
     3421 ttcagctacc gggtctgggc gagaaagagc cgcaagtcaa ccggggactc cagccggccg
     3481 caccggttca gtgccctgcg tccgggcgaa gcctcctatt acggtcctgg gctttacggc
     3541 aacggggtcg cctgtggcgg cacgctcacc ccgaccacgc gtggcgtcgc caacaagtac
     3601 ctgccctgcg gcaccagggt cacgcttcag tacggcaacc gcaccgtggt cgtccgggta
     3661 atcgaccggg ggccgtacgg tgccggccgg gactgggacc tgaccgagca gaccaagaag
     3721 gacctcgggt tcggcggcgt aggcaccgtc tggacgaata aatagtcggc gccggcttcg
     3781 ccgggcctcc cggtgaaacc cgtccgttca cggaatcaat gaatggatga acggactccg
     3841 gcttcggttg ggccgggctc acttcggcat aatccggagc atgagctcgg ccgaatcgaa
     3901 gaacctcgag gcgatctcga aggcgattga ccatcacaac gccacctgcc cctttccggc
     3961 ggccgaggtg cggatgaatc ccttcgaggt cgagcggctg ggctgggagg agatccgcgg
     4021 cctgccaatc gtccccgacc ccaacatcgg caccggacgc ttccacatcg tctgctcccg
     4081 cgacatggaa ggcgacgaac tcgaagaggt cgaggcgatc gcggaggagc tggtgacggt
     4141 cacgcccggg aagagggata cgccggccga accgggccag gagccggggg aacccgggat
     4201 cccggacaac ccggggcgct ggaactgaac agctcccctt gatccccggc ggagcgcgga
     4261 ctagtctgct ctcatgagat cccgggggag ggtatgggtt ttgctggccg gaatgctgct
     4321 ctcggcagcg atctggacct cgtcggctga agcggggcgg atcgcctacg tcgccgatta
     4381 cgacgcgaac gtcgtgtcgc agctggacac ggaaacgaac gccttcctgt ccacttcctt
     4441 tagcggaatg accgatacct acatgctcca ggtgtctccc gacgggaagt acctgtggat
     4501 cccgaggttc accccgaaca aggtttcggt ggtcgacctg accgacggcc aggcgctgcc
     4561 ggacatcgat gtcgatcaga acccggtcgg ggtggtcttt tcccccgaca gttcgatggc
     4621 ctacgtgctc agttacgacg ccagcacggt ccaggtcttc gacacggcca cccgccagca
     4681 gatcggcgat gacattccgg tcggggccgc cccctactac gagtcactct ctccggacgg
     4741 aagccgtctc tacgtcgcga acttcgacga cgacacgatc tcggtcatca acaccgccga
     4801 ccgccaggtg gtcggcgatc cggtcccggt ctgcgcggac ctgtactcga tggccatttc
     4861 accggacggg acccggctct tcatcggctg tgccggtagc ggagacatgg tgatgcagct
     4921 ttcgaccgac accatggaag cgacgagctt ctcggcgacc ctgcccgagg atcccgagta
     4981 cctgaccatc agtcccgacg gggccaccgg ctgggcggtg atcaggacgc tcgacgaggt
     5041 cgtttccttc agtaccgcga ccggggccgc gatcggcgat ccgatcccgg tcggccatga
     5101 gccgtatcac ctggcgatca ccccggacgg gtcgaaggca ttcacttcga actacgaggg
     5161 cacccagggc atcactccga tcaacatcgc gacccgggtt gccggaacgc tgatccccga
     5221 caccggcggt ccctggcaga tggcgatcac ccccgatcag ccgccgtcgg cgttgatcgc
     5281 ggcgccgatc agcatcgtgg ccggacagcc ggcgaagttc gatgccgtca actcgatcga
     5341 cccggacggc acgatcagcc ggtacgactg gaacttcggt gacggcaaga gcaagcccga
     5401 cggcaaggtg accgaaagtc acgtattcac gaaggcggga cagttttccg tgtcgctgat
     5461 cctgaccgac aacgagggct gctccaccgg gttcgtgacc gccgggtaca ccgcttactg
     5521 caacggcggg gccggggcat cgacgacgct caatgtcagg gtgaaagcgc catccaacaa
     5581 cttcagcttc ggcaggatca aactgaaccg gaagcgggga acggcatccc ttcaggtcag
     5641 gctccccggc cccggtgtcg tgatgctggc cggaaagaac gttcgtggtg cccgcaagac
     5701 ggtgaagaag gcaggcaagt ctgccctgag cgttcggatc acgggcaagg cgaagcgggc
     5761 gctaagcggc aggggcgtcg tcaaggtcaa ggccaaggtg acctacaggc cgaagtacgg
     5821 caagccgcgc accaaaacca gaacggtgac cctgcgccgg aactgatccg gcctcagacc
     5881 gagttccaga aatcgagacc ggtttcctgg gaccactgct ggatctcgat gttgatgcgg
     5941 gagagctcgc cggtccagat caggattccc atcgcgatca ggaccagacc gccgaccatg
     6001 atcacgaccc cgtaatgccg tttgatcaca gcgaaggcgc tggtcatctt cgagaaggca
     6061 agggcggtga gcaggaaggg gatcgccagg ccggccgaat agacggccag cagcagggcg
     6121 cccttggcgg ttgagtcggt ggtcgaggcg gcggagagaa tcgcgcccag ggtgggtccg
     6181 atgcacgggg tccaggcgaa ggcgaaggcg gcaccggcaa ccagcggccc gccctttccg
     6241 gcccgctgca tcagtgcgtc gacatgccac tccctgttca ggcgggtaac gaagaaggac
     6301 gcgacgaaga ggacgccgag cgcgatgatc agcgccccgg aaatcttctc cagaagctcc
     6361 tggttgtcgc tgaggagcga accgatcctg gtcgcaccca tcccgagcag gatgaagatc
     6421 agcgagaagc tgccaacgaa gatgaggctc ggggtgagca ccttcttcca gccggcgtca
     6481 tcgagctcgt tgaccgaaac cccggagacc gcagaaaggt agccgggaac gatcggcagc
     6541 acgcaggggg agaggaagga aacgaagccg gcgatgaacg cggtgaccag cccgaccccg
     6601 gcgacggagt caacggcggc aagtaccggg ctcacgaggc ccgcggaatt cagttctgca
     6661 aatggcaagg ggattagaga gtagtttggc acccatggaa cacagcctgg agggtgcagt
     6721 tgtcgtggta actggtggtg ccgccggaat cggccgggcc accgcgacga agatggccga
     6781 atcaggcgcg aatgtggcga tcggagacat ccacgcagag aaggccgccg aaacagcgga
     6841 agagatcacc tcaagggttt cggccggcgg gagcgggagc ggcgggggtg cgatcggccg
     6901 gccgcttgac gtcactgacc gcgacggctt cgagatcttt ctggatggag tcgaggccga
     6961 gttcgggccg atcgactgcc tcgtcaacaa cgccggggtg atgctgctcg ggccggtcga
     7021 tcaggaaagt ccggctgcca cgaaggcgat gatcgacgtg aacctgctcg gggtgatcaa
     7081 cggcacccag gctgcaatgg atcggatgaa gccacgggcc cggggccgga tcgtcaacat
     7141 cgcctcgcag gccggcaagg caggaatggc cggtggcgcc acctactgcg cgaccaagtt
     7201 cggcgtgatc ggcttctccg aggcggtgcg ggccgagctg aagggaaccg gggtttccat
     7261 ttcctgggtg ctgcccggcc tggtcaacac cgagctctcc gccggtttgc cagacattcc
     7321 cctgatggac cttctttccc cggatgacat cgcagacgcg gtgttgaagg ccctgacccg
     7381 gggtggcagc gagatctggg ttccggcccg caaccagtgg ctggacgccc cggcgaggct
     7441 gctgccgcgg ccgatgcgcg agaaggtttt cgcgatgacc ggcgccgaca aggtcctggc
     7501 cgaagctgat cccgataccc gccaggacta cgaggaccgg cattcgggag tctcctgaag
     7561 cccggccggt ttggcccgcc ggatagatga gcgggcctga ccttcggtaa actgccccgg
     7621 tgggctggcc cacctcggac agcggttccg accgctgcga atacgtgtct ccgacaacca
     7681 aacgccgcat ccgcggcctg aaaagcgcgc ttatgactga atttgccccg tcgtccgcga
     7741 cccgcgacgg cctctacgac tctacaaatg aacacgacgc ctgtggcgtc gccatggtcg
     7801 cgcgcctgga caacgagccc cgtcatgaag tggtcagcca gggcctgctg gccctcgaga
     7861 acctcgagca ccgtggagcc gccggggccg atgcctcgac cggtgacggc gccggcatcc
     7921 tgatccagct gcccgacctc tactaccgcg aggaagccgg cgtggacctg ccgccagccg
     7981 gccagtacgg ggtcgcgatg tgcttcctgc cgcgcgaaaa cgaagagcgc cgggaagaac
     8041 tcaagaccct gatcaccgag accaccgaag ccgagggcca ggtcgtgctc ggctggcggg
     8101 acgttccggt tgatcccggc cacgtcggca acacggcaaa cgattcccgg ccccatgtcg
     8161 cgcagctctt catcggggcg ggcgagtcac acaggagcga tcaggacgcc ttcgagcgca
     8221 agctctacgt gatccggcgg gtctgcgaaa tcgccgccgg cgaagagctt tacatcgcct
     8281 cctgttcctc ccgcaccatc gtctacaagg gaatgctgac cagctaccag gtgccgggct
     8341 tttacccgga gctccaggac gagcgggtcc gctcggccat ggccctggtt cactcgcgct
     8401 tttccaccaa caccttcccc agctgggacc tggcccatcc cttccgcctg atcgcccaca
     8461 acggcgagat caacaccctg cggggcaaca tcaactggat gcgggcccgg gaatcgcagc
     8521 tcgcctccga gctgttcggt gacgacctga agaaggtgac tccggtcgtg cggccggacg
     8581 gctccgactc ggcgaccttc gacaacgtgc tcgaactgct gatgctggcc ggtcgctcgc
     8641 tgccgcatgc ggcgatgatg atgatccccg agtcctacca gggccggacg gacctgagtg
     8701 acgagctgaa gggcttttac gcgttccact cctgcctgat ggagccgtgg gacggaccgg
     8761 cggcggtctg cttcaccgac ggccgcgtga tcggcgccac gctcgaccgc aacggtctga
     8821 ggcccggccg ctgggtcgaa accaatgacg gcatggtcgt gctcggctcc gagatcggcc
     8881 tgctcgacat cgcccccgag gacatcaagc gcctcggccg cctgcagccc ggcaagctct
     8941 tcctcgtcga tctcgagcag aagcggatca tcgaggacga ggaagtcaag cgggaagtcg
     9001 ccacccagca gccctacggc gaatggttcg agaacgcctc ggtccatttc gacgacctgc
     9061 cgaccgcgta tgtgacgatg accgggatcc agccgacccc gcgtcgccag caggccttcg
     9121 gctacaccca ggaggacctg cgggtcctga tcaccccgat ggccgcgacc ggtgccgaac
     9181 cgatcggctc gatgggcaac gatgtcgcgc tgccggtcct ctccgacaat cgtcccccgc
     9241 tttacagcta cttcaagcag cttttcgccc aggtcaccaa cccgccgatc gatccgatcc
     9301 gcgaggaaat cgtgatgagc ctctcggccg gggtcggcgc cgaacgcaac ctgctcgacg
     9361 aaagcgagca gcacgcccat cagctctgtc tcgaccagcc gatcctgatg aaccaggaac
     9421 tcgagacgat ccgccacgtc aaccacgaca tcttccgcgc ggacacgatc gacatcacct
     9481 gggacgtcgc cgaaggcacg gccggcatcg agtcccgcct tgacgcgatc tgcgaagagg
     9541 ccgaggaagc gatcgagaag ggcttcaaca tcctgatcct ttccgaccgc cggaccggcc
     9601 cgcgccgcgt cccgatcccg gccctgctcg cggtcggcgc ggttcaccat cacctggtcc
     9661 gggccggcac gcgcctgcgg gcgggcctga tcctcgagtc gggcgagccg cgcgaggtcc
     9721 atcacatggc gaccctgatc ggctacggct gctcggcgat caacccctac ctgatgctcg
     9781 actcggtcga cgacctggtc gtaaacggtc tcgtgccgga catgcagtcg ccggagaagg
     9841 cccagcgcaa tgtagtcaag gcgatcggca agggccttct gaagacgatc tcgaagatgg
     9901 gcatttccac cacccagtcc tacggtggcg cccagatctt cgaagcggtc ggcatcgaga
     9961 aggaagtgat cgacaagtac ttcaccggca cggcctcgag gatcggcggc atcggtctcg
    10021 acgtgatcgc ccaggagaca atggaccgtc acgcctcggc gttccccgga gccggcgacg
    10081 acctgctgcc ggtcggcggt gtgtacgcgt ggcgtcgtga cggcgagttc caccagtgga
    10141 acccggagac ggtgtcactg gtccagcacg cggtccgcgg cgaaggtggc tccgcccagg
    10201 agaaatacga cgagtttgcc cagcaggtca acgagatcgc cctgaagagg gccaccctgc
    10261 gcgggctctt caagctgaag gcgaaggatt cgggtccggt tccgatcgag gaagtcgaac
    10321 ccgctaagga aatcgtgaag cggttcgcga ccggcgccat gtcgctcggc tcgatctccc
    10381 gcgaaagcca cgagaacctg gcgatcgcca tgaaccgact cggcggcaag tcgaacaccg
    10441 gcgagggcgg ggaggacccg gtccggttca ccccggaaga aaacggcgac tcgcgccgct
    10501 ccgcgatcaa gcaggtcgct tccggccggt tcggggtcac cttccactac ctcgcaaacg
    10561 ccgaccagct ccagatcaag atggcccagg gcgccaagcc cggcgagggc ggccagctgc
    10621 ccggccacaa ggtcgaccgc tacatcggct cggtccggca cacaaccccg ggcgtcagcc
    10681 tgatctcgcc gccgccgcac cacgacatct actcgatcga ggacctcaag cagctgatct
    10741 acgacctctg ctgctcgaac ccgaaggcct cggtttcggt caagctggtt tccgaagtcg
    10801 gagtcggcac ggtcgcggcc ggcgttgcca aggcaaatgc cgaccatgtg ctgatcgccg
    10861 gccacgacgg cggcaccggc gcctcaccgc tttcgtcggt ccagtcggcc gggatcccct
    10921 gggagatcgg cctggcggaa acgcagcaga ccctgatccg caacgacctc cggtcccgga
    10981 tcacagtcca gaccgatggt cagctgaaga ccggccggga cgtcgtcgtc gccgccatcc
    11041 tcggcgccga cgagatgggc ttctcgaccg ctccgctgat cgcctcgggc tgcatcatga
    11101 tgcgggcctg ccacctcaac acctgcccgg tcggcattgc cacccaggat ccggagctgc
    11161 gcaagcgctt caagggccag cccgagcacg tcgtcaacta cttcttcttc gtggccgagg
    11221 aggcccggaa gatcatggcc gggatgggcg tacggtcgat cgatgaactg atcggccgga
    11281 tcgagctgct cgagaccgat gatgcggtcg accactggaa ggccaggggc attgacctct
    11341 ccggcctcct ggcctggccc gaggggatcg accactcggt cgaccgccgc cggacccgcc
    11401 cgcaggagcc ggtcctcgac gacaacctcg actggaagat tctggaacag gcgggggacg
    11461 cggtcgagaa cggcatcccg gtcgagttcg acatgaccgt ccagaacgtc gatcgctgcg
    11521 tcggtggcat cacctccaac gcgatcgcca acgcgcaggg cgccgagggt cttcccgagg
    11581 acacgatcaa ggtcaacttc cgcggcgccg ccggccagtc cttcggcggc tggctcgcgc
    11641 ccggcatcac cttctcgctg cggggcgagg tcaacgatta ctgcggcaag ggcctttccg
    11701 gcggcatcct cgcggtgtca ccgcccgatg gcgccgccta ccagccggaa gaaaacgtga
    11761 tcgtcggcaa cacctcgctc tacggtgcga ccaagggccg ggccttcttc cgcggcatcg
    11821 gcggcgagcg cttcgccgtc cgcaactccg gtgccaccgc ggtgatcgag ggcgtcggcg
    11881 accacggttg cgaatacatg accggcggca cggtggtcgt gctcggtccg accgggcgca
    11941 acttcgcggc cgggatgagc ggcggcatcg cctacgtgct cgacgagcag gacgagttca
    12001 gcggacgggt caacccgtcg atgagcaacc agctcgagga accggacgag aaggaccgga
    12061 tcgaactccg cggcctgatc caggagcacc tcgagcgcac cggttccgaa gtcgcccgcc
    12121 gcatcctcga tgactgggag tcgacctcgg cgaggttcgt caaggtcttc cccgaggatt
    12181 acaagcgggt actggccgag ctcgcagccg aaggcgagaa cgagccgggg gtcggacccg
    12241 aaaccaacga cgtcgaagtc attggcgagc cggccaccgg tgtcgcctcg gaaaagggag
    12301 cctgagcaat gggcaagctg ggaggtttcc tccagatcga acgtgccgga atcccctacc
    12361 gggatcccgc cgaaagggtc ggcgactaca acgagttcgt ggttcgcaga tccgatgagg
    12421 agctggccga ccagggcgcg cgctgcatgg aatgtggtgt ccccttctgc cacaacggct
    12481 gcccgctcgg caacctgatc ccggactgga acgacctcgt ctaccacgga cgctggcagg
    12541 acgcgatccg tcagctccac tcgaccaata acttccccga gttcaccggt cgcctttgcc
    12601 cggccccctg cgaagccgcc tgcgtgctcg agatccgcga gggcgaagcg gtaacgatca
    12661 agcagatcga gcagtcgatc gccgaccggg cctggaagga aggctggatc cggcctcgtc
    12721 cgcccaagcg cgagaccggc cgccacgtcg ccgtggtcgg ttcgggcccg gccggcatgg
    12781 ccgccgccca gcagcttcgc cggggcggcc acagcgtcac cctcttcgag cgggatgaag
    12841 ccgccggcgg cctggtccgc ttcggagtac ccgacttcaa gatcgaaaag accgtggttg
    12901 aacgccgcgt ccagcagttg atccacgaag gcgtggaggt caggtgcggg gtcgaagtcg
    12961 gggtcgatgt gaccgtcgag gaactgaagg aacgcttcga tgcggtcgtg cttgccaccg
    13021 gttcccgcgt tccccgtgac cttccggcgc cgggccgcga aaacgacggt gtccatttcg
    13081 cgatggacta tctctacagc cgcaatcgct ggctggcgtc cgaggtcggt cccaagccga
    13141 tgaccgaggc cccggcaccg gccccgattt cggcgaaggg caagcatgtc gtggtgatcg
    13201 gcggtggcga caccggcgcc gactgcgtcg ggcattcgat ccgcgagggc gcggccgagg
    13261 tggtccagct cgaactgctg cccgagccgc ccgagcatcg cccggatgac cgcaccccgt
    13321 ggccgctctg gccgaccaag ttccgcctct cctacgcgat ggaagaagcg aagggaatcg
    13381 aaaagggcga acaggacttc tccgtgacca cgacctcctt caacggggag gacggccagg
    13441 tccggtcgat cacggtcgcc aaggccgatc cgaacccgcc tttcgccgcg gttgaaggca
    13501 gcgaattcga gatcaaggct gacctggtcc ttctggcgat gggcttcctc catccggagc
    13561 aggaacttct cgacggtctc ggtgtggaca aggatcagcg cggcaacgtg aaggcaggca
    13621 cctacgaaac ttcggtgccc ggcgtctttg ccgccggtga tgcccgtcgc ggccagtcgc
    13681 tgatcgtctg ggcgatcaac gaaggccgcc aggccgcccg catggttgac cgcttcctcg
    13741 ggaccctgcc ttcgccgtca ccggtgccgc gcacgcccga cgtgccgacc ggcaacgtca
    13801 atccggccga cgaaggcccg gaaggaccgc caaagcacgt cgccccgggc cttggccccg
    13861 atcccagcgc ctcggccccc ggcttcgccc aggaatagct gccccgccac gagatccaac
    13921 tttttcgtca ttacgacgaa aaagtcacgc ctcactgatc gaatcaggtt cagtcggagg
    13981 gtgagccggc cgggggagtg cctgagggtg cttccgggcc ccagctcgct ctggccggac
    14041 cggtgatcgg gaccggctcg ccgccggtga ccggtgctcc gcggagcagg tcgaacacgg
    14101 ccttggtccg cgagagcacc tcgcgaatat cgctctttat gccctggccg ccgtaaccgt
    14161 gaaggtcgga ggtgaggccg ttcgcatcga ggcccgccgc ctgggcgagg aagagtgatc
    14221 tcttcatgtg aaagccctgg gtgacgacgg tcgcgtcgct caccccgaag atgtccctcg
    14281 cccgctgcat ggtggcgttg gtgttgaacc cggcgtggtc ctcgtagatc acattcgccg
    14341 ggattccctg ggcgacgagg gccttgcgca tcgtggtcgg ctcgtcatag gtccattggc
    14401 cgtggtcccc ggaaacgagg atcttctcga ccttgccgtc ccggtagagc gcggcggcct
    14461 ggtttacgcg gtcggccagc atcatgctca tcgtgccgtc ctggttgacc atcgcaccgg
    14521 gcacgattgc gacttcggcg tgcttggcat cggccgggtc attccagacc gtgccatccg
    14581 agtcgagcag gacgatcaga ttggtcgcaa gaacgaccag cagcgcgatg gttccgagcg
    14641 ccgctaccgt gatcagaccc cgcttcttcc acggcgaagg cctgcctgac ctgatcttcg
    14701 gcttagccag aacgggcagt gaatccgcat gtgaactggt gttcatggcc tccaaggtac
    14761 cagcgtccac caccggaaac gggtcatcgc aggcggtggg tcggatttcc ggcaggtgag
    14821 acggaaatcc gacgcacctc cgcgtagact cggaaacgcc ttggctcacg agcaccaaca
    14881 tggagatcac gggcacagcc acggccccgg cgcctggcgg gacgccgacc gcaaggccct
    14941 tctgatcgcg gccggactga ccgcgggatt catggtcgcc gaggtggtgg gcggcctgct
    15001 gaccgattcg ctggccctgc tcgccgacgc gggtcacatg ctctccgaca ccttttcgct
    15061 gttcctcgcg ctgggcgccg ttgcccttgc cgcccgcccg gtaaccgccc gccgtacctt
    15121 cggcttcaaa cgagcggaaa tcctcgccgc cctgatcaac ggcgtgctgc tggtcgtggt
    15181 ttcggtctgg atcgtgatcg aggcgatcga ccgcatcggc aatccgccgg aagtccttgg
    15241 cggctggatg ctggtcgtcg ccgtggccgg tctcgcggtc aacctgattg ccgcctgggt
    15301 gctgtcccgg tcggccggcg agagcctgaa cgtcaaggct gcgttgcgcc acgtgctggc
    15361 cgacgtggcc ggctcggtcg gcgtgatcct cgccgccctg atcatcctca ccaccggctg
    15421 ggagctggcc gacccgatcg tctcgatcgt gatctctgtc ctgatcgccg ccagcgcctg
    15481 gtcgatcctg cgcgattcgg tggacgtact cctggaggcg gcgccgccgg ggctcgacac
    15541 cgaggagatc ggctactcga tggcggccct gcccggcgtg gaccaggttc acgacctcca
    15601 catctggcag atcacctccg gaatgccgat gttgagcgcc cacgtgctgg tcggccccga
    15661 cagcgattgc catgcgatcc gcggcgatct cgaactgctg ctccgcgacg aatacgagat
    15721 cgaccacacc acgcttcaag tcgagcactc ggcgagccag gagccgatca cgatcgaagg
    15781 attcaacccg taggcgtgtc agatggggcg tccggggccg acgctacctt ctgaatcgtg
    15841 aactggtttg accggcggct cgccgccttc gacatagaaa ccaccggtac ggacatcgag
    15901 aatgaccgga tcgtcaccgc tgccgtcagc atcgtcggcg ggggcgagca gacggtcagc
    15961 cgaaactggc tggtcaaccc cggggtgccg atcccggcgg aggccaccga ggttcacaag
    16021 atcaccaatc agatggcgat gagggacggg gttccgaccg ccacggcggt tgaagaaatc
    16081 actaccctgc tggccggcgc gctcgaggaa gaagtgccgg tgatcgcctt caacgcccgc
    16141 tttgacctga ccatcctcga ccgggaggcg cgtcgtcacg gagtcgagcc gctggtcgac
    16201 agggttggcg ggccggatgg cctgctggtg gtcgacccgt atgtgcttga ccggcactac
    16261 aacaagttcc gcaagggccg caagaacctg acgatcctct gcgaggcttt cggggtcccg
    16321 ctgaccgagg cccacgccgc cgacgccgat gcgctcgcgg cagtccgatt ggcatggagg
    16381 atggcctcca gaaacgaaga gctcggccag atggagctgt tcgaactcca cgaaagccag
    16441 atccactggg ccgcggaaca ggcccgggac ctacaggagt tcttcgccaa aaatggccgg
    16501 gacgagcatg tcgaaggtgc ctggccggta gttccggcag cggccccggc agtgttcgaa
    16561 gaccgcctcg cggcctgacg ttcacgcgcc ttgccgccat cggccggttc gggctgagac
    16621 aatcggccgg tgagcgaagt tcacaggaca gaggatcagg tcgaagtcga gctcgaccag
    16681 aaagaggaga tcaacctcct tgacgagctg ggcggacccc agggaatagc cgattcgagc
    16741 attcccgggc tggtcttcgt catcgcctac acggtcagtg ggcagaagct ggaactggcg
    16801 gccggggtcg cggtcggaat cgcgttgctg atcgcgctga tcagactggt ccggggggag
    16861 cgggcccgct atgccgcttc cggcttcttc ggtgtcgccc tggctgcctt cattgcgacc
    16921 cgaaccggca aggccgagga tttctttctt ccggggctgc tgttcaacgc cggttactcg
    16981 ctcgcatacg cgatttcaat cgcgatccgc tggcccctga tgggcgtctt catcgggccc
    17041 ctgatcgggg agggaatgac ctggcgtgaa gatcccgagc gggtcaagct gttcaacaag
    17101 atcagctggc tttgggtcgg aacctttctg cttcgcctgg ccgtgcagtt gcccctctat
    17161 ctttcgggag cactggtcgc tctgggaatc gcgaaaaccg cgatgggact gccgatcttt
    17221 gcgcttgcga tatatctcag ctacctgatg ctgaaagccg ccggcatcga ccttcagcag
    17281 aagaaagaag ccgaagaagc gggctgatcg ccgggcgtga acggccccgc cgcggcaaag
    17341 gcatacaatg tctggccgct tttgtctccg tcttcatcac ccaccagaac cggcgaggcg
    17401 ccaggtcgcg ccggcgccaa tggcgacgcg aacccgcgcc cgaagcgcaa ggacgttccg
    17461 atccgacttc gggtcggcgc ttccgaaggc ctgtaccgga ttcgcccctt cgaaatggtg
    17521 gtccggaccc acccaccgga gaggccgggc gagtaccggg acgtctccga gctctggacc
    17581 ctcgacaagg acgagttcgg ttcacgcggc cggatcgaca ccacgatggg ggtcggcatg
    17641 gccgccgcgg cctgggcgcc gctccgcagc ctgctcgaag cggatgtgcc ggaccggctc
    17701 gaactcagtg acgctgaggt gctggacctg ctggacggcg ccgccgagag gctcgaggca
    17761 tccggcatcg tgctggattg ggacccggac ctggaccaga gcctgaccgg caaggtggtg
    17821 gtgggaagca gcggacggtt gagccagagc gttcccgacg tgttcgacca gaacgcgctg
    17881 cagggctttt cctggcagct ctcgctcgga ggaactcccc tgagcgagga ggaaatggcg
    17941 gtgatcgccg ataccgacgg gccgatcgcc cgactccggg aaaaatgggt gctggtgccg
    18001 cgggtgatgg ccgaacgggc gcgccatccc gatctgaagc ccctgaccgc gatcgacgcc
    18061 ctcagcgtcg ccctgaccgg cagcacggtc gtggacggcc agcgggtgga cgtcgaagcc
    18121 aagggctggg tagcccagct tcgtgaacag gtgactggcg acagcgaaca gtcgctgatc
    18181 gaaccgccgg ctgacctcaa cgcgaccctg cgcgagtacc aggtggaagg catgcgctgg
    18241 ctccaccgaa tgacctcgct cggtcttggc ggctgcctgg ccgatgacat gggcctcggc
    18301 aagaccgtaa tgctgatctc gcttcacctt caccggcggc tcaatcccca gaccgcggga
    18361 ccgacccttg tcgtctgccc ggcctcgctg cttggcaact gggaacggga gatccagcgc
    18421 ttcgccccga ccgagaaggt tcggcgctac cacggcccga accgcagcct cgaccagatc
    18481 accagcgggt tcgtgctgac cacctacggc accatgcggc tcgacgccga actgttcgag
    18541 gccgtgccct gggggcttct ggtcgccgac gaggcgcagc acgtaaagaa tccgcgctcg
    18601 gccggtgcca aggcgctccg cacgatcccg gcccgctccc gcgttgccct gaccggcaca
    18661 ccggtggaga acaacctctc cgagctctgg gcgattctgg actggaccac tcccggcctg
    18721 ctcggcagcc tcggtgcgtt ccgcaagaag tgggccgacc cgatcgaggt cgagggcagt
    18781 caatcggcca cccgtctgct caatcgcctg gtccggccgt tcctgctcag gcgacggaag
    18841 tcagatccgg gcatcgcacc ggagctgccg ccgaagacga tcaccgacca gccggttgaa
    18901 ctgagccccg aacagatcaa gctctatgaa gctgcggtcc gcgagaacat gctgcagatc
    18961 cgtaccgccg agggaatcaa ccgccgcggc ctggtgctga agctgctcac ttcgctgaag
    19021 cagatctgca atcacccggc ccagttcctc ggggaagaag ggggaccgct ggccgagcga
    19081 tccggcaagc tcgaactgct cgacgaactg ctcgacacca tcctcagcga ggacagctcg
    19141 gtgctggtct tcacccagta cgtggcgatg gcccgtctgc tgtcacgcca cctcgaagag
    19201 cgcggaatcg gcgcgatgct gctccacggc ggtaccgcgg tgaggcaacg cgaacgcatg
    19261 gtcgaacgct tccaggatgg cgaggtaccg gtcttcctgc tttccctgaa ggcggccgga
    19321 accggactca acctgacccg ggctgaccac gtgatccatt acgaccgctg gtggaacccg
    19381 gcggtggaag agcaggcgac cgaccgcgcc caccggatcg gccagctcaa ctcggtccag
    19441 gtccaccgcc tgatcgccga gggcacgatt gaagaccgca tcgcctcgat tcacgagacc
    19501 aagcgtgaac tcgccgatgc gatcgtcagc accggtgaag cggccctgac cgagctcagc
    19561 aacgaagacc tggccgaact ggtcgaactg aggaacgcgg gctgatgggc ggacaggccg
    19621 aaggcagccg cacctggcgg gggcagacga tcgaggaacg ccaggccggc cggcgggagc
    19681 ggctgatcga agccgggatc gagctgttcg ggaccagggg gtatgcgaac acgccggtca
    19741 aggcgatctg tgaggaagcc gggctgaccg agcgctactt ctacgaggcc ttcgaggacc
    19801 gggagagcct gctggacgag atctactcga tggtgctggc cgattgccgg cgggcgacgg
    19861 tggaagcgat cgacggcgcc ccggatgact tcgaagcctc gatgcgggcc gggctcaccg
    19921 ctttcgccca cgaatggatc cgtgatccgc ggagggcccg catccaggag atcgaggtgg
    19981 tcggggtcag ccccgaaatc gagcgcaagc gccgggactc gatccatgcg tttgccggga
    20041 tcgtggtcga ccgcacgctt cagctgggcg gcagggaaca gctcggcgcg ctgcggcttg
    20101 acgtgatcgc gctcggattg atcggatccg tgaacgaaca gctgatcgac tttgtgctcg
    20161 gagacctgca gatttccctc gatgacctga tcgagaacca gatcgccgcc tttgccgcgg
    20221 tggccagcca gcttctggaa tagcccccgg cgaggtgaca atgcttcgtg tcaggtctgc
    20281 gagaatgacc tcatgagcga acagcaagtc ggaccgaaga cgatcgtgat taccggtgcg
    20341 gcgaggggca tcggccttgc caccgcccgg attctcgcca cgcggggcaa ccgggtcgtg
    20401 atgtcggacc tcgacggcga acttgccgca gcagaagcag agaagatcgg ggatcgcgcg
    20461 gcaggattcg gcgtcgacgt gaccgaccgg gacgcggtcc gggaacacct cgagatggtc
    20521 gagagggagt tcggtccggt tgacgtgatg atcaacaacg ccggcatcgc ttccgcgtcg
    20581 ccgaacatcc tcgaacagga tccgaagatc accgagcgca ctattgacat caacctgaag
    20641 ggtgcgatga acgggaccat cgaggcgatt cgcctgatgg aacccaggcg ccggggccag
    20701 gtcgtgaacg tggcttcgct ggccgggatt atcggagtca aaggcctcgc tgcctactcc
    20761 gcatccaagt tcggtctggt cggattcacg gaatcgatcc ggctggaatt cgccgacaag
    20821 gggatcaaac tcacctgcgt gatgcccggt ccggtagaca ccgggatgat ggacggcacc
    20881 tcttcctcgc cgctggtttc gatgctcgca cccgaagaga tggcaaccgg aatcgccgac
    20941 gcagtgacca ccggcaagcc gcgggtctcg ctgccacgat cgagctggct gctggcccgc
    21001 acgatttcgg tactgccacc ggcgatcggg atccgggtcg ggcactggac gcggatcgac
    21061 cggatctaca ccgaaattga cccggccgcc agggccgcat acgaagaacg aatcagccgc
    21121 tgatcaatat cgtgggtcca tggaccggga cccgggtggc acaaggcttt caggtcgcga
    21181 aaggacctcg aatggccgcc cgagcgcgga aacggttgag gcgatctggc ggatcgagtc
    21241 cgcccggctc gttgcaaggc tctcgcggct gaccggggag gtcggcctgg ccgaggacat
    21301 ggcccaggaa gcgttcgtcg cggccctcga agtctggccc gggtcgggca ttccccgcaa
    21361 ccccgcagcc tggctgatga ccaccgcgag aaaccggggg atagaccgga tccggaggga
    21421 cagggtcctg accgaaaagt acggccagat cgcccatgag atggagcttg ccggacccgg
    21481 gcccggcccg gagcaggcgg tgttgaacga ggaccggatc ggcgatgacg tcctgaccct
    21541 gctcttcacg gcctgccatc catgcctgac caccgaaaca caggtcgcgc tgactctgaa
    21601 ggcggtcggc ggcctcacca ccgatgagat cgcccgggct ttccttgtct cggactcggc
    21661 gatcgcccag cgcatcgtcc gggccaagcg caccctcgcc gattccggga tcccctttga
    21721 gccgccgtcc accgacgaga tgggcgagcg gctggcggcc gtccttggtg tgatctacct
    21781 gatcttcaac gagggctatt cggcaaccgc cggcgatcaa tggatgcgca ccgagctttc
    21841 cgttgaggcg ctccggctgg gccggatcct ggtcgggctg gctccccggg agccggaggt
    21901 gcacgggctt gtgtccctga tggagattca ggcttcgcgc ttcccggcgc gaaccggccc
    21961 gcaaggggaa ccggtgttgc tgatggacca ggaccgctca cgctgggacc ggctcctgat
    22021 cagaaggggg ctgaaagagc tggagactgc gaggagcctt ggcgggaacg ggccgtacgt
    22081 gatccaggcg tccatcgctg cctgccatgc ccgtgcgttg aggccggagc agaccgactg
    22141 gcaggagatc cttggctact acgacgagct ggcggcgctg ggggattcac cggtcgccga
    22201 gctcaaccgg gcagttgcga tggcgatgtc cggagacccc cggtcggccc tcgacgcggt
    22261 ggatcgggtg gcgacggaca gtcgcatgaa gggctattac cttcttccga gcgtgcgcgg
    22321 tgatctgctg gagcggcttg gccggagcgg ggaggcggcg gccgagttcg aacgcgccgc
    22381 ttcactgacc gataatcagg cggaacggcg ggtactgatc gagcgggccg aacgggcaaa
    22441 agattttccg ggctgatgtc gaattccagc cttcccgttc gtcttcgatg caaaggccgg
    22501 aaaagccggc cgtctaccga aggagattga gatgcgttgc atggtgatcg tcaaggcaac
    22561 cgaggattcc gaagcagatg tgatgccatc cgagaaggac ctgggtgaca tggggagttt
    22621 caacgaggag ctggtcaacg ccggggtcat gctggacggc gacggattga agtcatcccg
    22681 tcacggggtc cggatcacct actccggaga tgaagcccag gtgaccgacg gtcccttctc
    22741 cgagaccaag gagctgatcg ccggatactg ggtctgggag gtgaagtcgc tcgaggaagc
    22801 gatcgagtgg gcacgccgga tccccttcca tcacggcgag tcggtcgaga tccggccctt
    22861 ccacgaactc gaagacttcg gcgaggcggt aaccgacgag ttccgggaac ggcaggcccg
    22921 gatggaagca acgatcgaag agcagaagcg ggacgcctga cgaaaagagg gggcccggag
    22981 gccccctctt tcagaacttc tgccggccgg acctacttgc cggacggcag tcgcgattcg
    23041 aagaacgaca tgacgtcgtt ctgggcggct gccacgactc cgccgtggtc gacgccttcg
    23101 tacttcttgt agtcgaccgt gtcgcccttg ccgaccagct cggtgtcgag ctggtcggtg
    23161 aggaacggga aggtggtcga atcggccgtg ccctgcgcca gcaggatcgg agccgaagtc
    23221 gtaaccgacg ggttctgcgc ttcgaggatc ccgttgagcg gggtcatgtc atagcccgag
    23281 cggatcagct cggaaggcgc gatgcccccg aggttgtcag accctgcgag ctccgacagg
    23341 cacttggtca gcgtctgcgg gtagaagggc agaatcgtgt cggaaagcgc ttcattcgcg
    23401 ttcagctgcg ggtaggagat gctcatgccg cgaaccagca gcgtggccag ggcggtcagt
    23461 ccgttggggg aggtgagtcc cgggaggaat gaggcctgct ccttgaagtg cgaagccggt
    23521 gcgtaggaaa cggtgcccct cagcttgatg tccttcgagt agctcgaggc aagaccggcg
    23581 gcgaacagtg ccgcgtgccc gccctgcgaa tggccggcga tcaggtacct gttgccgatc
    23641 ttcagaccga gctgacgcga ggcgctgacg atgtcaagca cgctgcggcc ggccggaacg
    23701 ccgaccagga acgggtggtt cacaccgggc acgccaaggc cctgataatc ggtgcgggca
    23761 accgcgtagc cggccctcag ccaggcattc tgcagcggat ccatgtacga gatgtaaggg
    23821 tcggcgacgc cacccttggt gttacgcgag ggggcgcact tgtcggcgat gccggtggtg
    23881 ccgtgggcgt aactgatgat cggccagccg cccttcgggg ccttgccctt cgggatgctc
    23941 acagaacccg acacggcgat ctgcttgccg gtggtcgacc tggaggtgta gagaaccagt
    24001 ttggtcgaag cggcgctctt cagaggcaca gacccaccgg ccttgcgctg ccagatcagc
    24061 ttgccgtggg cggtcgggag cttcttcggc ggcgagtaga acttcagccc ggccggaccc
    24121 tgctttggcc ccttcttctt tttcgtcttc tttgctgcgg catcggctcc ggccggctgg
    24181 gcgacctcga tcagcagggc cgtggtgagc agcatcgcca gggccaggac aacggcgcgc
    24241 aggcgggtca gttcaaaagt caagttcttt ctccctcaaa ggttggcagt cgatcggatg
    24301 ttccgacccg gaaactctac tcatcaaccc gtcggaggtg ccaaaggtgc gatgcatcag
    24361 aagcgagtag cgttgcgtga atgaccgaca tcctttcccg gcgcgacctt cagtttcagc
    24421 tcaacgaatg gctcgacgtc gagcggctga ccgagcgcga ccgctaccgc gaccagactc
    24481 tcgacgactg gaacgacgtg ctcacgcttg ccgagcagat cgccgaggac cagttccagc
    24541 cccacaatcg caaggcggac ctgaacgaac cggcgatcgg tgaagacggc aaggtcgtct
    24601 tgatcccgga agtcgcccag gccctggaga cacttggcga aaccgggctg atggccgcgg
    24661 cgatgccgga agaagtgggc ggcatgtctc tgccggccac cgtttcgagc gcgatctttg
    24721 cctggctcca tgccgccaac gtcggcacca ccgcctaccc gatgctgacc gccggggccg
    24781 ccaacctgct gatcgccaac gcctccgaag agcaggttga aacctgggtc aagccgatgg
    24841 tcgaggggcg ctttcacggc accatgtgcc tttccgagac ccaggccggc agctccctct
    24901 cggacatcac caccaaggcg gtcccgcagg acgacggcac ctaccggatc ttcgggaaca
    24961 agatgtggat cagcggcggc tcacacgagc tcggtgacaa catcgtgcat ctcgtactgg
    25021 cgaagatccc
//
LOCUS       DAOQKN010000013        24312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_857_length_24312_cov_5.891042, whole genome shotgun sequence.
ACCESSION   DAOQKN010000013 DAOQKN010000000
VERSION     DAOQKN010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 24312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24312
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_857_length_24312_cov_5.891042"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1245
                     /locus_tag="PLJ64_01805"
     CDS             <1..1245
                     /locus_tag="PLJ64_01805"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HNC92260.1"
                     /translation="LKGGDPFIFGRGGEEAEELADAGIPFEVVPGVTAAIAVTATAGI
                     PLTHREDSAGVAFVTGHRDGEADAGIDPDGSLALFPGTLAFYMGVKRLAENAADLISG
                     GRPADQPAAAIERGATPHQRVVTATLGTIAETVEREGIRPPALIVIGDVVRHHEKLAW
                     FEKRPLFGRTVVVTRARKQAGRLAAMLRGQGAEVIEMPVIESETIEASDPRIEEPFRQ
                     LREGAYDLVCFTSPNGVSAFFELIAGAGLDARVLAGATVAAIGPGTARTLRPRGIEPD
                     LVPDRAVAEGLLERLESEALEGKRVLIPRAETARDVLPDGLESRGAEVTIMPLYRTVP
                     VQPDDSVIKAAASADAMTFTSASTVSSLLAAVPGGLPGPVNVSMGPITSEALREAGLE
                     VAIEAGSGSLEGLVNATAKVLS"
     gene            1242..2285
                     /gene="hemE"
                     /locus_tag="PLJ64_01810"
     CDS             1242..2285
                     /gene="hemE"
                     /locus_tag="PLJ64_01810"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HNC92261.1"
                     /translation="MSEAAEAPLLVRALRREPVERKPVWFMRQAGRSLPEYRELREGA
                     PMLEMCRTPDLCAEVTLQPVRRHGVDAAIFFSDIVTPLEGIGIDVEIQSGVGPVIAEP
                     FRSEADLARLRPLEPDADLPEIAEAIRIVTAELGDTPLIGFAGAPFTLASYLVEGRPS
                     KSQEMARTMLLEEPGLFRNLLERLADIAVASLRSQVEAGVQAVQVFDSWAGSLSEATY
                     REAVLPVSQRLFAEIEDLGVPRIHFAVNAGHLMEAMAEAGSDAMGVDWRTPLSEARRR
                     LGEGVALQGNLDPTVPLAGPEVLERETRNLLADAPERGHVFNLGHGVLPSTDPGMLTE
                     LVGLVHEITEKRN"
     gene            2282..3211
                     /gene="hemH"
                     /locus_tag="PLJ64_01815"
     CDS             2282..3211
                     /gene="hemH"
                     /locus_tag="PLJ64_01815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HNC92262.1"
                     /translation="MSDETAKTGVLVMAYGTATGPDDVEAYYTHIRHGRPPEPEALKE
                     LQERYEMIGGSPLLGITQAQAEGIAAALERSNPGEFTTAMGQKHSAPFIEDAVRELLD
                     RGVERIVGIVLAPHFSTMSIAQYRSRAEEAAAEAGVPVTVIESWHEEPGYLEFLNQAI
                     TDAYEALPAEARESVELAVTAHSLPVAILERGDIYAEELERTRENLAGRPEFTGTTLA
                     WQSAGRTDAEWIGPDICEIMPGLKEAGRTAVVVCAAGFVSDHLEVKFDIDYEAKQAAD
                     EAGLALTRTAMPNDDPGFCATLAGIVAREAGKS"
     gene            3208..4245
                     /locus_tag="PLJ64_01820"
     CDS             3208..4245
                     /locus_tag="PLJ64_01820"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC92263.1"
                     /translation="MKRAVVVGAGIAGLTAAWQLSRSGHEVTVLDRAGRTGGRILPAE
                     FSGRLVDTGPDALGPDPALDELLEQIGMAGQVEIPDPARAFRLTAAGELEPYGVGGGA
                     MFGLFGGIFSLPARLEELLREAGQKFRLGVTVDSVRQEDRSVLVEAEGSKEAFDLAVV
                     ATGARAAGRLLDGETGERLAAIRGVSVSLVHLAFPEKAIGREMNGTGYLADPAEGRLV
                     TGCSWSSAKWKRLAGDPAILRASIREAGQGEVIGLPDREVVEKVMAELVPVMEIEGDP
                     VETMVTRHPEALVVRDEEHESAVAAARGLLAATPRIGLAGADLDGPGISRSIAGVRET
                     VRAIVEGTRND"
     gene            4238..4903
                     /locus_tag="PLJ64_01825"
     CDS             4238..4903
                     /locus_tag="PLJ64_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HNC92264.1"
                     /translation="MTDTEETLHYCAYLSYSWDVEAGRGLAPAVEPLVAEIEAAGVEI
                     RGTYATTAFRAECDILFWLLADSTLKLDAAARAIRTSGLAPALTPYWNAIGVHQEAEF
                     NRAHVPAFVAGEPAGDFICLYPFVRSLEWYLLEDEERRELLAEHGMLARDYKDVRANT
                     VSTFGLSDYEWLLAFEASEPVRIVQLMRELRAARARLHTREELPFLNGIRRPLAEIVK
                     GLE"
     gene            5000..5857
                     /locus_tag="PLJ64_01830"
     CDS             5000..5857
                     /locus_tag="PLJ64_01830"
                     /inference="COORDINATES: protein motif:HMM:NF015213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HNC92265.1"
                     /translation="MLPTFVIALREGVEASLIVGIVAAFLVQQGRRDMLRHVLVGVGI
                     AVLICTGIGIGLQLLNEELPQRQQEMLETVIGLAAVAIVTFMIFWMRRHARGMKSELE
                     SSASRALAEGSAGALIGMAFFAVLREGIETVVFLLAIFQGAEDATAAGLGAVLGILSS
                     IAIGYGIYRGGVRLNLSRFFKLTAVVLVFVAAGLLSNSVHTAWEAGWVTFLQGQAVDL
                     SWLVVPGTWTSALLTGMLGLQSTMTEAQVIVYLVYLLPMLAYVLWPEAWRVRRPRNKV
                     ARAPNPADA"
     gene            5892..7052
                     /gene="efeO"
                     /locus_tag="PLJ64_01835"
     CDS             5892..7052
                     /gene="efeO"
                     /locus_tag="PLJ64_01835"
                     /inference="COORDINATES: protein motif:HMM:NF007697.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HNC92266.1"
                     /translation="MPLRSKHDDRHPLGILLLGFVLLAASAVLLAACGSDESGGSAAQ
                     KIAVEITDEGCQPTDLKAKSGPATFTVTNTASDVNNEFEVLDGALIVGERENLVAGLS
                     GDVSVNLDPGTYDIVCGNPGNREPTGRLVVTGGNVAATTNPGLSRAVDEYADFVVEQS
                     NILERRASAFVAAVKAGNVPLAKRLYETSRIPYEKIEPVAESFGTLDPAIDARENDIA
                     QGDPWTGYHPLEKALWATGLTAADDRLADQLLVNIKRLQKRVKANTYDPVQLANGAVE
                     LLNEVSQSKVTGEEERYSHIDLTTFKANVDGAKEAFDLLEPELKKVDPDLAREIEQRF
                     NDLYAALDKYQVKGDVYENYSKVNQQERRELSQKVDALAQPLALMGGQIVTQ"
     gene            7049..8299
                     /gene="efeB"
                     /locus_tag="PLJ64_01840"
     CDS             7049..8299
                     /gene="efeB"
                     /locus_tag="PLJ64_01840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HNC92267.1"
                     /translation="MTPEGDSPGGHITRRRLLGAGAAGGLAVAGFGVGRATAPDPPDP
                     EQSVVSFEGAHQAGIITPAQNRLHFAAFDLTTNRRSDLQQLLREWTAASRRMISGEMA
                     GPVNGEPLLPPDDTGEALGLGPANLTITFGLGPGIFDRRFGLASKRPPQLEPLPPLPG
                     DELDPNISGGDLCIQACADDPQVAFHAVRNLTRIASGMAVMRWCQLGFGRTAKTGAAQ
                     ETPRNLMGFKDGTNNILNDDQQALDEFVWAGGEGPDWMRGGSYLVARRIRMLIEAWDR
                     TFLQEQEEVIGRAKESGAPLGEAKEDDLVDLKATGSDGKPVIRQDAHIRQAAPSVNDG
                     QKILRRGYSFTDGFDRVRGQLDAGLFFICFQRDPRKQFVPIQERLGKLDGLMEYIVHD
                     GSGLFAVPPGVSEPDGFIGQGLFS"
     gene            8334..9299
                     /locus_tag="PLJ64_01845"
     CDS             8334..9299
                     /locus_tag="PLJ64_01845"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HNC92268.1"
                     /translation="MRGISKASGAWSRNVAIAVAAGLGLAGLFLAPSASAGLSCRYVA
                     AGPDGPRGNRLEIKATRFEEVVALLPGKGSNIEVTDDQRMKPLACKGGKPTMVNIDRV
                     NFTADRRATGSSIYIAEAPEFGPGATPAEAGGKGITFIAKGPSLSFGIGGTDGPDLIQ
                     MGMEGKAAALDFFPDLYPDSESGDNIDAKIYAKFVNLLVRGGRGADAIAGSQIDYSST
                     WFSGPLRVPTSIYGEVGPDALTGGKSMDYLDGGPGGDFMLGAQGADQLFGGGGIDTFI
                     AGPGRDEIDALDGIGGEDLRCGRGRDLARMDLKDQDRDCESFRFP"
     gene            9277..10128
                     /locus_tag="PLJ64_01850"
     CDS             9277..10128
                     /locus_tag="PLJ64_01850"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HNC92269.1"
                     /translation="MSPSVSPENANRGQTRPVTFLTDFGYSDEYPGVCRLVIASIDPR
                     IKVIDLTHGIPPGDIRRGGLALAAAVRFSAPAVHLAVVDPGVGSRRRAVVLTCGEHHL
                     VGPDNGLLSLAAAVLGGVEAAFDVSATPVRVGGSDTFHGRDVFSPVAAELARGAAPGE
                     LGEAIGFRELAPLELPEPLVTPEQVDAHVLYPDQYGNVILNIHSEVLDGTFFTEGDAI
                     SLETSLQKLEVPLRRTFADVEPGEPVLFTDSSGYLALAINRGSAASRFGLGPDNQLRF
                     TPVASRQ"
     gene            10125..10835
                     /locus_tag="PLJ64_01855"
     CDS             10125..10835
                     /locus_tag="PLJ64_01855"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNC92270.1"
                     /translation="MIIGRPHLHYRQVDSTNERARQVAAGGAPDGTLVTAAEQTAGRG
                     RQGRPWSTPAGSAVAWSVLIRRQVEIPGTLPLQVGTGVCDAVEALGASGVQMKWPNDI
                     WIDGRKCAGILVEARPQDGWAVAGVGLNLSIEDDEFPEELRGRATSLGGGVTFTEAVA
                     ALNERVGRSIDRPVEETLAAFADRDGLRGRQVSWAEGQGTAVGIDPQGNLLVESTAGC
                     IATLNAGEVHLSLPVDAA"
     gene            10832..11395
                     /locus_tag="PLJ64_01860"
     CDS             10832..11395
                     /locus_tag="PLJ64_01860"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HNC92271.1"
                     /translation="MSAGAGSPLRGFHPGAGSDAAGGPIDFTRSGVVWLEGEAVLIEP
                     TGTGKPLARLRDGRLVVGGGGRAQVLAAIDRWYRREARQRIEPLVEVEGQRLGLEAGK
                     VRIGNQRTRWGSCSTSGTVSFNWRLVLGRPEALHYVVVHELMHLRHHNHSRDFWNDLA
                     AAFPGFREEARWLRVNERRLLQWKPRI"
     gene            11377..12600
                     /locus_tag="PLJ64_01865"
     CDS             11377..12600
                     /locus_tag="PLJ64_01865"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNC92272.1"
                     /translation="MEAADLIRYRGRMIYGGRARVGNLNLAAAAVAIAAVALLALFLP
                     GNPASASTRSNLNQALNRVMKAPNGPPAISMQIVKNGHSQYYRRGVADLRTGAKPRLR
                     MRFRIASVAKAFSGAVALNLVARGKLSLHSTIGEVLPGMLPKAKQVTLGQALHHTGGL
                     PEYIKSKGFLNEVGNDPGAFVPPTRIISWVRGSGLTHRPGTKYEYSDTDNIVVGLMAQ
                     RVTGVRYAWQIRRLGRQIGGLTATSLPRTLRMPEPYIHGYEIEAGQQPNDVTRLVNPS
                     GAWASGGIVSTLPNLSRFFRAYVAGRFFGQGAKARAIKRAQRHWIRGESQPAGPGNNW
                     AGMGLFRYVTKCGVMYGHTGSFPGYRAFAASSADGKRSIAFVANAQIVPGRGSQKVNN
                     RIRRMQVAAVCRALR"
     gene            complement(12602..13036)
                     /locus_tag="PLJ64_01870"
     CDS             complement(12602..13036)
                     /locus_tag="PLJ64_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92273.1"
                     /translation="MSRNLKLTLSSVLVAGLLLAGCGTDKQESVSVSLETFTSPDGNI
                     GCIADQTMVRCDIKHKTWKVKRDPKCQLDYGGGLTVADGKAELVCAGDTTLNSGPDVA
                     AGTVNMVGPFECQTNEWGDSMRCENVTTGNGFELSADKYSTF"
     gene            complement(13005..15614)
                     /locus_tag="PLJ64_01875"
     CDS             complement(13005..15614)
                     /locus_tag="PLJ64_01875"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNC92274.1"
                     /translation="MAPTTAAPAATAAPSKKDIERASEFARESLLLNGDGPDSLIPGT
                     ARRRAFDQAIAELDAGLTEPSVEWRRNFSLLLGLERLIGEEPPTLKDGAELNPHQIDA
                     LSGTLTELLAEQQVSAVDDIETLEVLEEEGDTPLRDESEAEAEPSGEDDEPEDGDEAD
                     EVIDEAVDDDEEPLDWEDKADEDSGDEEEVGEAADDPGAARRFWFEHATGAGKTVAAV
                     GFIEATRTGGVLILTHRRNLVDQFIGEISDRGYKDRLRPPLLDGRDEADGPVTVETYQ
                     WFVRNAKRVSDAYSVIICDEAHTALGEKTSACIRSMPGPVYIGMTATGALIARHVADL
                     FPTQTSRFDLAQAARRGVIAPLRCLRIPPGPGVRTIAKVPLRKGEVDQDFDQEELAKL
                     LDQAPFNMAIADLYKSRFRKAPGVLYTAGVQHAKNVAAAFREFGINAKAVSGETPKRE
                     LSDTLAAFERGEVDVLCNAQLLAEGWNSPRATICMHLAPTASKRIYQQRVGRVTRRSP
                     NKEAGLVIDFVHPATTHDESIVTLHSLLDRDVYRGGAIVVGPVRRGRGRKVRVERRIV
                     PVSDDPQKRIEVLERDIWRIAVENLNHSEQHVWAALAGARVTSQGWRRAKAMLQHDRT
                     KELRRRYLLTCVQRNRNAQLRVKALSEIANLKDPEAFDDAIDIVGTWSRDEKRAGTKV
                     LLQALVDKRIGRRDQAQAWVWRLASYTRELHEEYAVQRWPETKRLLGLLVNSAGRAHG
                     KNARKLVQAARKQDRRLAVSLLAAAVAHTPEGEEVLRGARMRMARKPSAVARELLRNF
                     PKGNKKRRRKKNKKKDQAENPEVESTETTDTSAEGEATGEVEEVQEVDEVDETETTEE
                     AEPDEPESEADPE"
     gene            15771..16364
                     /locus_tag="PLJ64_01880"
     CDS             15771..16364
                     /locus_tag="PLJ64_01880"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNC92275.1"
                     /translation="MKSAVHRHLTVLALLGALLGGLVLAGCGEEGNEPSPGPNYEKAL
                     AGAPPRLAKLYDQANELLPGGLDAFDERVQTDLAGYPAVVNIWASWCGPCRAEFPHFQ
                     QVSARMGKKLAFLGVNSDDNSDAAKTFLSTHPVPYPSYEDPDKDIAQSLGATHGLPGT
                     AFFNADGELTYTRSGPYSSDQELEADVQKYAIEGQSG"
     gene            16408..17022
                     /locus_tag="PLJ64_01885"
     CDS             16408..17022
                     /locus_tag="PLJ64_01885"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNC92276.1"
                     /translation="MEISDPTLAYLLIILGLTGIGIEAITPGGFVPAILGIIALVFGV
                     IGVVDIGPTAIGLGLLLLAIALFISAIAFHLYRPLSIAGVISLVASGIWMFDRETDPT
                     SIIAVTIGAVALGLFMLFVIERASETRAAPVRYGPEDLVGMPGEVRVPLRPDGQVFVD
                     GALWQARLADSTARLGVGEKVTVTEVSGLTLLVAPASETNQGEI"
     gene            17025..17867
                     /locus_tag="PLJ64_01890"
     CDS             17025..17867
                     /locus_tag="PLJ64_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HNC92277.1"
                     /translation="MAVVGTLVVILAIFALIVLFSAIKILREYERGVIFRLGRLINQK
                     GPGLILLIPFIDRMVKIDLRTVTLNIPPQEVITRDNVPTSVNAVCYFRVVDPNRAITD
                     VENYLIATSQIAQTSLRAVLGKAELDEILSERERLNESLQKIIDEQTEPWGVKVTTVE
                     IKDVEIPEAMQRAMARQAEAERERRAKIINSEGEYQAAEKLTDAADIISRNPASLQLR
                     YLQTLLEISGNQNSTVIFPLPMDLISAFQQTLGNIGNADGGSDGGVQPTIGGARPDEL
                     SSEP"
     gene            17875..18945
                     /locus_tag="PLJ64_01895"
     CDS             17875..18945
                     /locus_tag="PLJ64_01895"
                     /inference="COORDINATES: protein motif:HMM:NF013269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92278.1"
                     /translation="MSSPEVRIDQLTGGRVLVAPGRSERPDDFTPGAWHPAGHDGCPF
                     CEGNESKTPPEVEADRPEGGEANGPGWVTRTVPNLYPAVAPRESGEAADQRKVAGRAG
                     TESSAFSSPADPLLASGRGSEPDMFASRPAYGSHEVIVNSPFHLASLAELEEQSLAAA
                     VAAWRSRMRAHSDSAYVQLILNQGPDSGASLEHSHAQLGAMEFVPPSVARERERVGAY
                     RERTAGASLLGEILREEVRRGERLVAIDEEVALICPWASRHPYEMRLVPRRPVARFEE
                     DEGGAAMLGRAIRALTELFGNPCQLNLWVRTAPRGVEHFHWHLDLIPRLEPASAFEMA
                     TGVDINIVSPETAAETLRDAID"
     gene            18959..19663
                     /locus_tag="PLJ64_01900"
     CDS             18959..19663
                     /locus_tag="PLJ64_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92279.1"
                     /translation="MPIDNRPFPRFVADASRHGIPHGRFAERLATAFKDACREIDDLP
                     AGTEIPEEFTWFPERAWSGRVWVPVSADGEAVLEENSEPEPIEFFGFVSFVQPEGGDP
                     TDFKASADFTDVVAADNPDWTIDISDEVIGTWLGEQGREAAMTLVWGRALVRDAFAVT
                     AELDDVTVDQDPLFGGQGGRADRFTLIAPDALKNYGDDAYLEIKLWTSRGKQVARESL
                     YEIEEPQTAPEQEPAE"
     gene            19775..20935
                     /locus_tag="PLJ64_01905"
     CDS             19775..20935
                     /locus_tag="PLJ64_01905"
                     /inference="COORDINATES: protein motif:HMM:NF037429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VLRF1 family aeRF1-type release factor"
                     /protein_id="HNC92280.1"
                     /translation="MKNQKRVEELRELIDQESEGPVLSVFCRTDPRDPANSSETPAWA
                     IALKNGLNHVAGFHEGNHGKEQTVKKLCAEAERRINGASAADRGRSVALFIGGDGKVD
                     SFTTFQIPVREDYVTVDSGPVVWPMLDVIDRGQRTGLVLLSSDRIRLLEWWDGRTRDL
                     EESTFDLELGDWHQYRGPARPQGTVGGGGVSNASAYDDRVEVWRARFVKAAAKAIAES
                     ASELGFDRLVAAAEGELGAGFNEALPADMRSRLATTVHLNLIDLPAAEAGAALDPHLR
                     QAWRDGVSGDGARAMERSRAGGRGAAGADRVLAALGEGRVEHLILDPYLKTDELTLDS
                     ASRQAIEDAGEESVQEALVELALRTGARVSSASVDEVPALTEAGGVLALLRY"
     gene            21006..21079
                     /locus_tag="PLJ64_01910"
     tRNA            21006..21079
                     /locus_tag="PLJ64_01910"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21040..21042,aa:Arg,seq:cct)
     gene            21140..21544
                     /locus_tag="PLJ64_01915"
     CDS             21140..21544
                     /locus_tag="PLJ64_01915"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNC92281.1"
                     /translation="MAKMKRKPISTRKPPVPADDSTDVDDWTSRVMPDLQPIVASLDQ
                     QIRKAIPDLQYAVKWKKAYYGREDLGWVIEMVAYDISVNMVFLGGADFDSPPPLGDTD
                     RSRYIKIKSLEEAETPEMGAWIEQAGRVPGWK"
     gene            21566..21967
                     /locus_tag="PLJ64_01920"
     CDS             21566..21967
                     /locus_tag="PLJ64_01920"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNC92282.1"
                     /translation="MPFTRINLKQDVDNFGPNFDGSPDLDFRAATKPLELEHSFLGYQ
                     RIPPGYRFPFGHTHDSQEEVYVVVGGSGRMKLDDEIIELAKWDAVRVPPGTWRGYEAG
                     PDGLEILISGAPNLGDQVRDDVTGQRDWWQD"
     gene            21975..22475
                     /locus_tag="PLJ64_01925"
     CDS             21975..22475
                     /locus_tag="PLJ64_01925"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HNC92283.1"
                     /translation="MADWADDFFVEWQRAWGTGTEAVLEFVTDDIEYWDVTLPEPIRG
                     REAYAGHCDNFFTAFTECEWSLREPVICEGDRVTEPWAFTGLNSGPIWIGLPATGRRL
                     ESKGTDIFEFRDGKVCREHSFYDVVGNLRSLGLWPGIGSRTEAAAMTAAGAAIRGTGM
                     VRNLRP"
     gene            22633..23988
                     /locus_tag="PLJ64_01930"
     CDS             22633..23988
                     /locus_tag="PLJ64_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNC92284.1"
                     /translation="MTEIDRGRLAELTQSELDRFVEENPKSKELFERARKSLVGGVPM
                     PWMMRWAGGFPVFAKLAIGARIIDVDDHTYIDFCLGDTGAMPGHDPEPVVRAITDQER
                     KGITTMLPTEDAPWVGEELTRRFGLDRWMFTLTATDANRAALRICRQITGKPKVLVFS
                     YSYHGSVDEAFAVAGPEGVTVSREGNVGAPVDPAQTTFAVEFNDLEALEAALATGEVA
                     CVLAEPAMTNMGIVLPDEGYHEALRNLTRKHGALLIIDETHTFSAGPGGCTAAWGLEP
                     DLLSIGKAIGSGVPAGALGMTEAVTESLFAQQDADYEDTGGVGGTLAGNALSLAAVRA
                     TLSEVLTEEAFAHTVPLATRFSEGVMQVVTDAGLPWNVTQLGARAEYRFEPEPARNGT
                     QAHNASDPELERYLHLHALNRGVLITPFHNMALMSPATIPADVDRHTEVFKEAVLDLV
                     G"
     gene            24119..>24312
                     /locus_tag="PLJ64_01935"
     CDS             24119..>24312
                     /locus_tag="PLJ64_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC92285.1"
                     /translation="MEASMENKPTVAIITGASSGIGEGVAWRLARKPGTRLILVARRE
                     DRLRELAKRLSCEVTWIAADL"
BASE COUNT         3994 a         8169 c         8193 g         3956 t
ORIGIN      
        1 ctgaagggcg gcgacccgtt catcttcggc cgcggcggcg aggaagccga agagctggcc
       61 gacgccggga tccccttcga ggtcgtgccc ggtgtgaccg ccgcgatcgc ggtgaccgcc
      121 acggccggta tcccgctgac ccaccgcgag gattccgcag gggtcgcctt cgtcaccggt
      181 caccgggacg gtgaggcaga tgcgggcatc gaccccgacg gttcgctggc cctcttcccg
      241 ggcaccctcg cgttctacat gggggtgaag cgactggctg aaaacgccgc cgacctgatc
      301 agtggcggcc ggccggccga ccagcccgcc gcggcgatcg aacgcggcgc aactccccac
      361 cagcgggtgg tcaccgcgac cctgggcacg atcgccgaga cggtcgaacg ggaaggcatc
      421 cgcccgccgg ccctgatcgt gatcggggat gtggttcgcc atcacgaaaa gctcgcctgg
      481 ttcgaaaagc gccccctctt cggtcgcacg gtcgtggtca ccagggcccg caaacaggct
      541 ggccggctgg ccgccatgct gcgcggccag ggcgcagagg tgatcgagat gccggtgatc
      601 gaatcggaaa cgatcgaagc ttccgatcca cgaatcgagg agccgttccg ccagttgcgg
      661 gagggtgcct acgacctggt ctgcttcacc agtccgaacg gagtctccgc cttcttcgag
      721 ctgatcgccg gggccgggct ggacgccagg gtgctggccg gcgccacggt tgccgcgatc
      781 ggtccgggca ccgcccggac cctccggccg cgcggcatag aacccgatct ggttcccgac
      841 cgggcggtgg cggaaggtct gctggaaagg ctcgagtcgg aagcgctcga gggcaagcgg
      901 gtgctgatcc cccgggcgga aactgcccgt gacgtcctgc cagacggtct cgagtcccgc
      961 ggcgccgagg tcacgatcat gccgctctac cggaccgtcc ccgtccagcc ggatgattcg
     1021 gtgatcaagg cggcggcaag cgccgacgcg atgaccttca cttccgcctc gaccgtttcc
     1081 agtctactgg ccgcggtgcc tggcggcctg cccggaccgg tcaacgtttc gatggggccg
     1141 attacctccg aggcacttcg cgaggccggg ctggaagtgg cgatcgaggc cgggtcgggc
     1201 agcctcgaag gcctcgtgaa cgcaaccgca aaggtccttt cgtgagtgag gctgccgaag
     1261 ctccgttgct ggtccgggca cttcgccggg aaccggtgga gcgcaagccg gtctggttca
     1321 tgcggcaggc cggccgctcc ctgccggaat accgcgaact ccgggaaggg gccccgatgc
     1381 tggaaatgtg ccggacgccg gacctctgcg ccgaggtcac cctgcagccg gtccggcgcc
     1441 acggggttga cgcggcgatc ttcttctccg acatcgtcac cccgctggag gggatcggca
     1501 tcgacgtgga gatccagtcg ggggttggcc cggtgatcgc ggaaccattc aggagtgaag
     1561 ccgacctggc ccggttgcgg ccgctggagc ccgatgcaga cctgccggag atcgcggaag
     1621 cgatccggat cgtcaccgcc gagctcggtg acacgccgct gatcgggttc gccggggcgc
     1681 cgttcaccct ggccagctac ctggtcgagg gccggccctc caagtcgcag gaaatggccc
     1741 ggaccatgct gctggaggaa ccggggcttt tccggaacct gctcgaacgc cttgccgaca
     1801 ttgcggtcgc ttcgcttcgc tcgcaggtgg aggccggggt gcaggcggtg caggtcttcg
     1861 actcctgggc cggctcgctt tccgaggcga cttaccggga ggccgtcctg ccggtctcgc
     1921 aaaggctctt cgccgagatc gaggatctgg gggtgccgcg gattcacttc gcggtcaacg
     1981 ccggccacct gatggaggcg atggccgagg ccggctccga cgcgatgggg gtggactggc
     2041 ggaccccgct ctccgaggct cgccgccggc tcggtgaggg cgtcgccctg cagggcaacc
     2101 tcgatccaac cgtgcccctg gccgggccgg aagtgctcga gcgcgagacc cgcaacctgc
     2161 tggccgacgc tcccgagcgg ggtcatgtct tcaatctggg tcacggagtc ctgccttcga
     2221 ccgacccggg catgctgacc gagctggtcg gccttgttca cgagatcacg gagaagagga
     2281 attgagcgac gaaacagcga agaccggagt cctggtcatg gcctatggaa ccgcgaccgg
     2341 gccggatgac gtcgaggcct attacacgca catccgccac ggccgcccgc ctgagccaga
     2401 ggccctgaag gagcttcagg aacgctacga gatgattggc ggatcaccgc tcctgggcat
     2461 cacccaggcc caggccgaag gcatcgccgc cgccctcgag cggtcgaacc cgggcgagtt
     2521 cacgaccgcc atgggccaga agcattcggc ccccttcatc gaggatgccg tgcgggagct
     2581 gctcgaccgg ggagtggagc ggatcgtcgg catcgtcctg gctccgcact tctcgaccat
     2641 gagcatcgcc cagtaccgca gccgggccga ggaagccgcg gcggaagccg gggtgccggt
     2701 caccgtgatc gaatcgtggc acgaggaacc gggctacctc gagttcctga accaggcgat
     2761 caccgacgcc tacgaggcgc tgcccgcgga agcccgtgaa tcggtcgagc tggcggtgac
     2821 cgcccacagc cttccggtgg cgattctcga gcgaggcgac atctacgccg aggagctgga
     2881 gaggacccgc gagaacctcg ccgggaggcc ggagttcacc ggcacgaccc tggcctggca
     2941 gagtgccgga cgcaccgacg ccgagtggat cggcccggac atctgcgaga tcatgcccgg
     3001 cctgaaggaa gcggggcgga cggcggtggt ggtctgtgcc gccgggttcg tttccgacca
     3061 cctcgaggtc aagttcgaca tcgactacga ggcaaagcag gcggccgacg aagcgggact
     3121 ggccctgacc cgcaccgcca tgcccaatga cgatccgggc ttctgtgcca cgctggccgg
     3181 gatcgtcgcc cgggaggccg gcaagtcttg aagcgggcgg tcgtcgtcgg agccggcatc
     3241 gccgggctga ctgcggcctg gcaactcagc cgatccggcc acgaagtgac ggtgctggac
     3301 cgggccggcc ggaccggcgg ccggatcctt ccggctgaat tctcggggcg gctggttgac
     3361 acgggcccgg acgctctcgg tcccgatccg gcgctggacg aactgctcga acagatcggc
     3421 atggccggtc aggtcgagat ccccgacccg gcccgggcct tcaggctgac cgcggcgggt
     3481 gagctggagc cctacggggt cggcggcggg gcgatgttcg gtctcttcgg cgggatcttt
     3541 tccctgccgg ccaggctgga ggagttgctg agggaagccg ggcaaaagtt ccgcctcgga
     3601 gttacggtcg attcggtccg gcaagaggac cggtcggtgc tcgttgaagc ggaaggatct
     3661 aaggaggcat ttgacctggc cgtggtcgca accggtgccc gcgccgccgg gcgcctgctc
     3721 gacggggaaa cgggcgaacg gctggccgcg atccgggggg tgtcggtcag tctcgttcac
     3781 cttgccttcc cggagaaggc gatcggccgc gagatgaacg gcaccggcta cctcgccgat
     3841 cccgccgaag gccggctggt caccggctgt tcctggtctt cggccaagtg gaagcggctg
     3901 gccggcgacc cggcgatcct gcgggcatcg atccgtgaag ccggccaggg cgaggtgatc
     3961 ggtctgccgg accgggaggt tgtggagaag gtgatggcgg agctggttcc ggtgatggag
     4021 atcgaaggag atccggtcga gacgatggtc acccgccacc cggaggccct ggtcgtccgc
     4081 gacgaggaac atgaatcggc ggtggcggcg gctcgtggcc ttctggccgc gaccccgcgg
     4141 atcgggctgg ccggcgccga cctcgacggc ccgggcatat cgcgctcgat cgccggggtg
     4201 cgagagactg tcagggcgat cgtcgaaggg acacgaaatg actgataccg aagaaaccct
     4261 ccactactgt gcctaccttt cctattcctg ggatgtcgag gccggccggg ggctggcccc
     4321 ggcggtggaa ccgctcgtgg ctgaaatcga ggccgccggg gtggagatcc gcggcaccta
     4381 cgcgacgacc gcgttccgcg ccgaatgcga catcctcttc tggctgctgg ccgactcgac
     4441 cctgaagctc gacgcggcag cccgggcgat ccggacctcc gggctcgctc ccgccctgac
     4501 cccgtactgg aacgcgatcg gcgtccatca ggaagccgag ttcaaccggg ctcacgtccc
     4561 cgccttcgtg gccggcgagc cggccggcga tttcatctgc ctctatccct tcgtgcgctc
     4621 gctcgagtgg tatctgctcg aggatgaaga gcggcgggag ttgctggccg aacacggcat
     4681 gctggcccgc gattacaagg acgtgcgggc caacacggtc agcaccttcg ggctcagcga
     4741 ctacgagtgg ctgctcgcct tcgaggcgag tgaaccggtc cggatcgtcc agctcatgcg
     4801 ggagctgcga gcggccaggg cgcgcctcca cacccgcgag gaacttccct tcctcaacgg
     4861 gataagaaga cccttggcgg agatcgtcaa aggacttgaa taaggggtcg cgagacctcc
     4921 gggcgggctc tccccgagga ctcgatccga gccagagtta ggtataccga agtaatcctg
     4981 tataaacggg ttggttttca tgctgccaac ctttgtcatc gcactccgtg aaggcgtcga
     5041 ggcctctctg atcgtcggga tcgtggctgc ctttctggtg cagcagggcc gccgtgacat
     5101 gctgcgccac gtgctggtcg gagtgggtat cgcggtgctc atctgcaccg gcatcgggat
     5161 cggcctgcag ctcctcaacg aggaactgcc gcagcgccag caggagatgc tggaaacggt
     5221 gatcggcctc gccgcggtgg cgatcgtcac cttcatgatc ttctggatgc gccggcatgc
     5281 ccggggcatg aagtccgaac ttgaaagcag cgcctccagg gccctggccg aagggtcggc
     5341 gggtgccctg atcgggatgg cgttcttcgc cgtgctccgc gagggaatcg agacggtggt
     5401 ctttctgctc gcgatcttcc agggcgccga ggacgcgacc gcggccgggc tgggcgcggt
     5461 gctgggcatc ctctcgtcca tcgcgatcgg gtacgggata taccgcgggg gcgttcgcct
     5521 caacctgagt cgcttcttca agctgaccgc ggtggttctg gtcttcgtcg cggccgggct
     5581 gctctccaac tcggtccaca ccgcctggga agccggctgg gttaccttcc tgcagggtca
     5641 ggcggtcgat ctcagctggc tggtcgtccc cggcacctgg acctcggccc tcctgaccgg
     5701 gatgctcggc ctgcagtcca cgatgaccga agcccaggtg atcgtctatc tcgtctatct
     5761 gctgccgatg ctggcctatg tgctctggcc cgaagcctgg cgggttcgca ggccgcgcaa
     5821 caaggtcgcg cgcgcaccca acccggccga cgcctgagtc tcctccggcc ggtcgccccc
     5881 gaaaggaatc aatgcccctc cgatcgaaac acgacgatcg tcaccccctc ggaattctgc
     5941 tcctcggctt tgtcctgctt gccgcttcag ccgttcttct ggccgcctgc ggctccgatg
     6001 aatccggcgg ttctgcggcc cagaagatcg cggtcgagat caccgacgag ggctgccagc
     6061 cgaccgacct gaaggcaaag tcgggacccg ccaccttcac cgtcaccaac accgccagtg
     6121 acgtgaacaa cgagttcgaa gtcctcgacg gtgcgctgat cgtgggcgag cgggagaacc
     6181 tggtcgccgg tctttccggc gacgtctcgg tcaatctcga tcccggcacc tacgacatcg
     6241 tctgcggcaa ccccggcaac cgcgagccga ccggcaggct ggtcgtcacg ggcgggaacg
     6301 tcgcggcgac gacgaatccc ggactctcca gggccgttga cgagtacgcc gacttcgtgg
     6361 tcgagcaatc gaacatcctc gagcggcggg cgagcgcatt tgtcgccgcg gtaaaggccg
     6421 gcaacgtgcc cctcgcaaag cggctttacg aaacctcgcg catcccctac gagaagatcg
     6481 aaccggtggc cgaatccttc ggcactctcg atccggcgat cgacgcgagg gaaaacgaca
     6541 tcgcccaggg tgacccgtgg accggctatc acccgctgga aaaggctctc tgggcgaccg
     6601 ggctgaccgc cgccgatgac cggctggccg accagctgct ggtcaacatc aagcgcctcc
     6661 agaagcgggt caaggcaaac acctatgacc cggtgcagct ggccaacggt gccgtcgaac
     6721 tgctgaacga ggtgagccag tcgaaggtca ccggggaaga ggagcgctac tcccacatcg
     6781 acctgaccac cttcaaggcg aacgtggacg gcgcgaagga agccttcgac ctgctcgaac
     6841 cggaactgaa gaaggtcgac cccgacctgg cccgcgaaat cgagcaacgc ttcaacgatc
     6901 tctacgccgc gctcgacaag taccaggtca agggtgacgt ctacgaaaac tactcgaagg
     6961 tgaatcagca ggagcgtcgc gagctcagcc agaaggtgga cgcgctggcc cagcccctgg
     7021 ccctcatggg tggccagata gtcacgcaat gaccccggaa ggcgacagcc ccggcggcca
     7081 catcacccgg cgccgactgc ttggcgccgg agcggccggc ggcctggccg tcgccgggtt
     7141 cggggtcggg cgggcgaccg caccggaccc gcccgacccg gagcagtcgg tggtctcctt
     7201 cgagggggcg catcaggccg ggatcatcac cccggcccag aacaggcttc acttcgcggc
     7261 ctttgacttg accacgaacc ggcgctccga tcttcaacag ttgctcaggg agtggaccgc
     7321 ggcgagccgg cgcatgatct cgggcgagat ggcaggcccg gtcaacggcg agccgctcct
     7381 gccgccggat gacaccgggg aagcgcttgg cctggggccg gccaacctga ccatcacctt
     7441 cggcctcggg ccgggaatct tcgaccggcg tttcggcctc gcatcaaagc gaccgccaca
     7501 gcttgagcct ctgccgccgc ttcccggcga tgaactcgac ccgaacatca gcggcggcga
     7561 tctctgcatc caggcctgcg ccgacgatcc ccaggtcgct ttccacgcgg tccgcaacct
     7621 gaccaggatc gcctcgggga tggcggtcat gcgctggtgc cagctcggct tcggccgcac
     7681 cgcgaagacc ggcgccgccc aggaaacccc gcgcaacctg atgggcttca aggacggaac
     7741 caacaacatc ctcaatgacg accagcaggc actggacgag ttcgtctggg ccggcggcga
     7801 gggcccggac tggatgcgcg gcggcagcta tctggtcgcc cgccggatcc gcatgctgat
     7861 cgaagcctgg gatcgcacct tcctgcagga gcaggaggag gtgatcggcc gggccaagga
     7921 aagtggcgcc cccctgggtg aagccaaaga ggatgacctg gtcgacctga aggcgaccgg
     7981 atccgacggg aagccggtga tccggcagga cgcccacatc cgccaggcgg cgccctcggt
     8041 caacgatggc cagaagatcc tgcgtcgcgg ctactcgttc acggacggct tcgaccgggt
     8101 ccgcggccag ctcgatgccg gtctcttctt catctgcttc cagcgcgatc cgcgcaagca
     8161 gttcgtgccg atccaggaac gcctcggcaa gctggacggg ctcatggagt acatcgtcca
     8221 cgacggcagc ggcctgttcg ccgtcccccc gggggtcagt gaaccggacg gcttcatcgg
     8281 ccagggactc ttcagctagc ccggccgcgc ggccgggcaa atagaatccg gccatgcggg
     8341 gaatcagcaa ggcaagcggg gcgtggagcc ggaatgtcgc gatcgcggtt gccgccgggc
     8401 tcgggttggc cgggctgttt ctggcacctt ccgcctcggc cggactcagc tgtcgctacg
     8461 tggcggcagg accggacggc ccgaggggca accggctgga gatcaaggcg accaggttcg
     8521 aggaagtggt cgcgctgctg cccggcaagg gctcgaacat cgaggtaacc gatgaccagc
     8581 gcatgaagcc gcttgcctgc aagggcggca agccgaccat ggtcaacatc gaccgggtca
     8641 acttcactgc ggaccgcagg gcgaccggat cgtcgattta catcgccgaa gcgccggagt
     8701 tcggtcccgg ggccaccccg gccgaggccg ggggcaaggg catcaccttc atcgccaaag
     8761 gtccgtcgct cagcttcggg atcggcggca ccgacggccc ggatctgatc cagatgggca
     8821 tggaaggcaa ggccgccgcg ctcgacttct ttcccgatct ctatcccgac agtgaatcgg
     8881 gcgacaacat cgacgcgaag atctacgcga agttcgtcaa cctcctggtt cgcggcgggc
     8941 ggggagccga cgcgatcgcc ggcagccaga tcgattactc gagcacctgg ttcagcggtc
     9001 cgctgagagt tccgacctcg atctacggcg aggttggccc cgacgccctg accggcggca
     9061 agagcatgga ttacctggac ggcggccccg gcggtgactt catgctgggc gcgcagggag
     9121 ccgaccagct cttcggaggc ggcggcatcg acaccttcat cgccggcccg ggccgggacg
     9181 agatcgacgc gctcgacgga atcgggggag aggatctgcg ttgcggccgc ggcagggatc
     9241 tggcccggat ggacctgaaa gatcaggacc gggattgtga gtccttccgt ttcccctgag
     9301 aacgcaaatc gggggcaaac gcggccagtc accttcctga ccgacttcgg gtacagcgac
     9361 gagtaccccg gggtctgccg gctggtgatc gcctcgatcg atccccggat caaggtcatc
     9421 gacctgaccc acggcattcc ccccggtgac atccggcgcg gcggcctggc cctggctgcg
     9481 gcggtgcggt tctccgcccc ggcggttcac ctggccgtgg tcgatcccgg agtcggcagc
     9541 cgccgccggg ccgtggtgct cacctgcggc gagcatcatc tggtcggtcc ggacaatggc
     9601 ctgctctccc tggccgccgc ggtgctgggc ggggtcgagg ccgccttcga cgtctccgcc
     9661 accccggtcc gggtcggcgg gagtgacacc ttccacggcc gtgacgtctt cagcccggtg
     9721 gcggccgaac tggcccgcgg tgcggccccc ggcgaactcg gggaagcgat cggtttccgg
     9781 gagctggccc cgctcgagct gcccgaaccg cttgtcaccc cggagcaggt ggacgcccat
     9841 gtgctttacc cggatcagta cggaaacgtg atcctgaaca tccattccga ggtgctggac
     9901 gggaccttct tcaccgaggg tgacgcaatc tcgctggaga cgagcctcca gaagctcgag
     9961 gtgccgctcc gccgcacctt cgccgatgtc gagcccggcg aaccggtcct cttcaccgac
    10021 tcgagcggct atctcgccct ggccataaac cgcggcagcg ccgccagccg tttcggactc
    10081 ggccccgaca atcaactcag attcaccccg gtagcctccc gccagtgatc atcgggcgtc
    10141 cgcatcttca ctaccgtcag gttgattcga ccaatgagcg ggcccgtcag gttgcggccg
    10201 gcggcgcccc cgacggcacc ctggtgaccg cggcggaaca gaccgcgggc cggggcaggc
    10261 aggggcggcc atggtcgacc ccggccggct cggcggtcgc ctggagcgtg ctgatccgac
    10321 gccaggtcga aattccgggc accctgccgc tgcaggtggg gaccggggtc tgcgatgcgg
    10381 tcgaggccct cggtgcttcc ggagtccaga tgaagtggcc gaatgacatc tggatcgacg
    10441 gtcgaaaatg cgccggaatc ctggtcgagg cgagaccgca ggatggctgg gccgtggccg
    10501 gggtcggtct caatctttca attgaagacg acgagttccc ggaggagctg cgcggacggg
    10561 cgacctcact tggtggcggc gtgactttca ccgaagcggt ggcggcactg aacgagcgcg
    10621 tcggtcgcag catcgacagg ccggtggagg agacgctcgc cgctttcgcc gaccgggacg
    10681 gactgcgggg ccggcaggtc agctgggccg aggggcaggg caccgccgtc ggaatcgatc
    10741 cccagggcaa cctgctggtt gaatcgaccg ccggctgcat cgcgaccctg aacgcgggcg
    10801 aggtccacct ctcgcttccg gttgacgcgg cgtgagcgcg ggagcaggtt ctccgctccg
    10861 gggctttcac cccggggccg gctccgatgc ggccgggggg ccgatcgact tcacccgctc
    10921 cggggtcgtc tggctggaag gggaggccgt tctgatcgag ccgaccggca caggcaagcc
    10981 gctcgcgcgc ctgcgtgacg gccggcttgt ggtgggcggg ggtggccggg cccaggtcct
    11041 cgccgcaatc gaccgctggt accgccgcga ggcacggcag cggatcgaac ccctggtcga
    11101 ggtcgagggc cagcggctcg gtctcgaagc cggcaaggtc cggatcggaa accagcgcac
    11161 ccgctggggc tcctgctcga cctccggcac cgtctccttc aactggcgcc tggtcctcgg
    11221 ccggcccgag gcactccact acgtggtcgt tcacgagttg atgcacctgc gccaccacaa
    11281 ccattcccgc gacttctgga acgaccttgc cgccgccttc ccgggattca gggaagaggc
    11341 cagatggctg cgggtgaatg agcgccggct cctccaatgg aagccgcgga tctgatccgg
    11401 taccgtggcc gcatgatcta cgggggcagg gccagagtcg ggaatttgaa cctcgctgcg
    11461 gcagctgtcg cgatcgcagc ggtcgccttg ctcgccctgt ttttgcccgg caacccggcc
    11521 agcgcctcga cccgttcgaa cctgaaccag gccctgaacc gggtaatgaa ggcgccgaac
    11581 ggaccccccg ccatctcgat gcagatcgtg aagaacggtc actcgcagta ctaccgtcga
    11641 ggcgtcgccg acctgaggac cggtgcgaag ccccggctgc gcatgcgctt ccggatcgcc
    11701 agcgtggcaa aggccttcag cggtgcggtc gcgctgaacc tggtcgctcg cggcaagctc
    11761 agcctgcatt caacgattgg cgaagtcctc cccggcatgc tgccgaaggc gaagcaggtg
    11821 actctcggcc aggcgcttca ccacaccggc ggcctgcccg aatacatcaa gtcgaagggt
    11881 tttctcaacg aggtcggcaa tgatcccggg gcattcgtcc cgccgacccg gatcatctcc
    11941 tgggtccgtg gatcggggct gacccaccgc ccggggacga agtacgagta ctcggacacc
    12001 gacaacatcg tggtcggcct gatggcccag cgggtgaccg gcgtccgcta cgcctggcag
    12061 atccgccggc ttggccgcca gatcgggggg ctcaccgcca cctccctgcc tcgcaccctg
    12121 cggatgccgg aaccgtacat ccacggctac gagatcgaag cgggccagca gccgaatgac
    12181 gtcacccgcc ttgtcaatcc ctccggcgcc tgggcttccg gcggcatcgt ctctaccctg
    12241 cccaacctga gccgcttctt ccgcgcctat gttgcgggcc gcttcttcgg ccaaggtgcg
    12301 aaagcgaggg cgatcaagcg ggcccagcgg cactggatcc gcggcgagtc acagccggcc
    12361 gggccgggca acaactgggc cggaatgggc ctcttccgtt acgtcaccaa gtgcggcgtg
    12421 atgtacggcc acaccggttc cttccccggc taccgtgcct tcgccgcctc ctcggctgac
    12481 ggcaagcgct cgatcgcctt cgtcgccaac gcccagatcg ttcccggccg cggctcgcag
    12541 aaggtcaaca accggatccg ccgcatgcag gtagcggcgg tttgccgcgc cctgcgctga
    12601 cctagaaggt cgagtacttg tcggccgaga gttcgaagcc gttgccggtg gtgacgttct
    12661 cgcagcgcat gctgtcgccc cactcgttgg tctggcattc gaacggcccg accatgttga
    12721 ccgtcccggc ggccacatcc gggccgctgt tcagggtcgt gtcaccggca cagaccagct
    12781 cggccttgcc gtcggccacg gtcaggccgc caccgtaatc aagctggcac ttcgggtccc
    12841 gcttgacctt ccaggtcttg tgcttgatgt cgcagcggac catggtctgg tcggcgatgc
    12901 agccgatgtt gccgtccggc gaggtgaaag tctcgagact gaccgacacg ctctcctgct
    12961 tgtcggtccc gcagccggca aggagcagtc cggcgaccag gacgctactc agggtcagct
    13021 tcagattccg gctcatccgg ttcggcctct tcggttgttt cggtctcatc aacttcatca
    13081 acctcctgga cttcttcgac ttccccggtt gcttcacctt ccgcggatgt atccgtggtc
    13141 tcggtgcttt cgacctccgg gttttcggcc tggtccttct tcttgttctt cttgcggcgc
    13201 cgtttcttgt tgcccttggg gaagttccgg agcagctcgc gggcgacggc cgaaggcttc
    13261 cgggccattc tcatgcgggc gccgcgcagc acctcttcgc cttccggggt gtgggcgacg
    13321 gcggcagcca gcagcgaaac cgcgaggcgg cggtcctgct tgcgggcggc ctggaccagc
    13381 ttgcgggcgt tcttgccgtg ggcgcggccg gccgaattga ccagcagtcc gagcaggcgc
    13441 ttggtttccg gccagcgctg gaccgcgtac tcctcatgga gctcgcgggt gtaggaggcg
    13501 agccgccaga cccaggcctg ggcctggtcg cggcgaccga tccgcttgtc gaccagcgcc
    13561 tgcagcagca ccttggtgcc ggcccgcttc tcgtcccttg accaggtgcc gacgatgtcg
    13621 atcgcgtcat cgaacgcctc gggatccttc aggtttgcga tctcgctcag ggccttgacc
    13681 cggagctggg cgttgcggtt gcgctggacg caggtcagca ggtagcggcg gcgcaattcc
    13741 ttggtccggt catgctggag catcgccttg gcccggcgcc agccctggga agtgacccgg
    13801 gccccggcca gggcagccca gacatgctgc tcggagtggt tcaggttctc gactgcgatc
    13861 cgccagatgt cccgttccag cacctcgatc cgcttctgcg gatcatcgga gaccgggacg
    13921 atgcggcgct cgacccggac cttgcggccg cggccacgac ggaccgggcc gaccacgatc
    13981 gcgccgccgc ggtagacatc gcggtcgagc aggctgtgca gggtgacgat cgactcgtcg
    14041 tgggtggtcg ccggatggac gaagtcgatc accagcccgg cttccttgtt cggcgagcgg
    14101 cgggtcacgc gaccgacccg ctgctggtag atccgcttcg aggcggtcgg ggccaggtgc
    14161 atgcagatgg tggcccgggg cgagttccag ccctcggcca gcagctgggc gttgcagagc
    14221 acgtcgactt cgccgcgttc gaaggcggcc agcgtgtccg agagttcgcg cttcggggtc
    14281 tcgccggaga ccgccttggc gttgattccg aactcccgga aggcggcggc gacgttcttc
    14341 gcgtgctgaa cgccggccgt gtagagcaca cccggggcct tgcggaagcg cgacttgtag
    14401 aggtcggcga tcgccatgtt gaacggggcc tggtcgagca gcttggcgag ctcttcctgg
    14461 tcgaagtcct ggtccacctc acccttgcgg agcggcacct tggcgatcgt tctgacgccc
    14521 gggccgggcg ggatgcgcag gcagcggagc ggggcgatca cgccgcggcg ggcggcctgg
    14581 gcgaggtcga agcgggaagt ctgggtgggg aagaggtcgg cgacgtggcg ggcgatcagg
    14641 gcgccggtcg cggtcatgcc gatgtagacc gggccgggca tcgagcggat gcaggccgag
    14701 gtcttctcgc cgagcgcggt gtgggcctcg tcgcagatga tcaccgagta ggcgtccgaa
    14761 acccgcttcg cgttgcggac gaaccactgg taggtctcga ccgtcaccgg gccgtccgct
    14821 tcgtcgcggc cgtcgagcag cggcggccgg agccggtcct tgtagccgcg gtcggaaatc
    14881 tcgccgatga actggtcgac caggttgcgg cggtgagtga ggatcaggac gccgccggtc
    14941 cgggtcgcct cgatgaagcc gaccgcggcg accgtcttgc cggccccggt cgcgtgctcg
    15001 aaccagaagc ggcgggcggc accgggatcg tcggcagctt cgccgacttc ctcttcatcc
    15061 ccggaatcct cgtctgcctt gtcctcccag tccaggggct cctcgtcatc gtccacggcc
    15121 tcgtcgatga cctcatcggc ctcgtccccg tcttcgggct cgtcatcctc tccggaaggt
    15181 tcggcttcgg cttcgctctc gtcccggagc ggggtgtcgc cttcctcctc gagaacttcc
    15241 agggtctcga tgtcatcgac cgcactcacc tgctgctcgg ccagcagttc ggtcagggtg
    15301 ccggaaaggg catcgatctg atgcgggttc agctcggccc cgtccttcag ggtcggcggt
    15361 tcctcgccga tcaggcgttc gagcccgagc agcagcgaaa agttccggcg ccactcgacc
    15421 gaaggttcgg tcaggccggc atcgagttcg gcgatcgcct ggtcaaaggc acggcggcgg
    15481 gcggtacccg gaatcagcga atcgggaccg tccccgttca gcagcaggga ttcacgcgca
    15541 aattcagaag cgcgctcgat gtctttcttt gagggagccg cagtagctgc gggtgctgca
    15601 gtagtcgggg ccaacttagc cttcgcaagt gttccgaacc cggattctcg acccggcccg
    15661 gatgagtagg tgatcgaaga ttccgacgac gacgctggac ggacagacgc cggtttcctc
    15721 cgtgatccgc gatacggcgg atcgaatgtc tgtatttaat cattacttgc atgaaatcgg
    15781 ctgttcacag acatctgacg gttcttgccc tgctcggcgc gctgctcggc gggctggtcc
    15841 tggccggttg cggtgaagaa gggaacgagc ccagccccgg gccgaactac gagaaggccc
    15901 tggccggggc accacctcgc ctggcgaagc tttacgacca ggcaaacgag ctcctgccgg
    15961 gcggactcga cgccttcgac gagcgggtcc agaccgacct ggccggctat ccggcggtgg
    16021 tcaacatctg ggcttcctgg tgcggaccct gccgggccga gtttccacac ttccagcagg
    16081 tctcggccag gatgggcaaa aagctcgcct tcctcggcgt gaactcggat gacaactcgg
    16141 acgcggcgaa gactttcctc tcgacccatc cggtccccta ccccagctac gaggacccgg
    16201 acaaggacat cgcccagtcc ctcggggcca cccatggcct gcccgggacg gcgttcttca
    16261 acgcagacgg ggaactgacc tacacccgtt ccggccccta ctccagcgac caggagctgg
    16321 aagcagacgt ccagaagtac gcgatcgagg gtcagtccgg ctgagcccgc ttactgccca
    16381 ccgggcagga ccgatacgct cgggtccgtg gagatcagtg atccgacact cgcctacctg
    16441 ctgatcattc tcggcctgac cgggatcggg atcgaggcga tcacgcccgg cggtttcgtg
    16501 ccggcgattc tcggcatcat cgctctggtc ttcggggtga tcggagtggt cgacatcggg
    16561 ccgacggcga tcgggctcgg cctgctgctg ctggcgatcg ccctgttcat ctccgcgatc
    16621 gccttccacc tctaccggcc cctctcgatc gccggagtga tttcactggt cgcctcgggc
    16681 atctggatgt tcgaccgtga gaccgacccg acctcgatca tcgcggtgac gatcggcgcg
    16741 gttgcgctcg gactcttcat gcttttcgtg atcgagcggg ccagtgaaac cagggcagcc
    16801 ccggtccgtt acggccccga ggacctggtc gggatgccgg gcgaagtccg ggtgccgctc
    16861 cggccggatg gccaggtctt cgttgacggc gcactctggc aggcccgcct cgccgactcc
    16921 acagcccggc tcggcgtcgg cgagaaggtc accgtcaccg aggtcagcgg cctgaccctt
    16981 ctggtcgccc ccgcctcaga aaccaaccaa ggagagatct agagatggca gtcgtcggaa
    17041 ccctggtcgt gatcctcgcg atcttcgccc tgatcgtcct gttttcggcg atcaagatcc
    17101 tgcgcgaata cgagcgcggc gtgatcttcc ggcttggccg gctgatcaac cagaagggtc
    17161 ccgggctgat ccttctgatc cccttcatcg accgcatggt caagatcgac ctgcgaaccg
    17221 tgaccctgaa catcccgccg caggaagtga tcacccgcga caacgtgccc acctcggtca
    17281 acgcggtctg ctacttccgc gtggtcgacc ccaaccgggc gatcaccgat gtcgagaact
    17341 acctgatcgc cacctcgcag atcgcccaga cctcgctccg ggcggtgctc ggcaaggctg
    17401 aactggatga gatcctttcc gagcgagagc gcctcaacga gtcgctgcag aagatcatcg
    17461 acgagcagac cgagccctgg ggcgtgaagg tcacgacggt cgagatcaag gacgtcgaga
    17521 ttcccgaggc gatgcagcgg gcgatggccc ggcaggccga ggccgaacgg gaacggcggg
    17581 ccaagatcat caactccgaa ggcgagtacc aggcggcgga gaagctcacc gacgcggccg
    17641 acatcatcag ccgcaacccg gcttcgctgc agctgcgcta cctgcagacc ctgctcgaga
    17701 tctccggcaa ccagaactcg accgtgatct tccccttgcc gatggatctg atctccgcct
    17761 tccagcagac gctcggcaac atcggcaacg ccgacggcgg ctccgatggt ggcgttcagc
    17821 cgacgatcgg tggcgccagg ccggacgaac tttcgtcgga accctgaaaa cgctttgagt
    17881 tcacctgaag tcaggatcga ccagctgact ggaggccggg tgctggtcgc gccgggtcgc
    17941 tccgagcgcc cggatgactt caccccgggg gcgtggcacc cggccggtca cgacggttgc
    18001 ccgttctgtg agggaaacga atcgaagaca ccgcccgagg ttgaagccga ccggcctgaa
    18061 ggaggcgagg cgaacggccc cggctgggtc acccgcacgg tcccgaacct ttacccggcg
    18121 gtggcaccgc gggagtccgg tgaagcggcc gaccagcgca aggtcgccgg tcgggccgga
    18181 accgagtcca gcgccttctc cagtccggcc gatccgctgc tcgcctccgg tcggggctcg
    18241 gaaccggaca tgttcgcttc tcggcccgcc tacggcagcc acgaggtgat cgtcaactcg
    18301 ccctttcacc tcgcctcgct ggccgagctc gaggagcagt cgctggccgc cgcggtcgcc
    18361 gcctggcgca gccgcatgcg ggcccattcg gattcggcct acgtgcagct gattctcaac
    18421 cagggccccg actccggcgc ttcgctggag cacagtcacg cccagctcgg ggcgatggag
    18481 ttcgtgccgc catcggtcgc ccgcgaacgc gaaagggtcg gggcctaccg ggaacggacc
    18541 gccggggcca gcctgctcgg cgagatcctg agggaggaag tccgccgtgg cgagcggctg
    18601 gtcgcgatcg atgaagaggt cgccctgatc tgcccgtggg cctcccgtca cccctacgag
    18661 atgcgactcg tcccgcgccg gcccgtcgcc cgcttcgagg aagacgaagg cggggcggcg
    18721 atgctgggcc gggcgatccg ggcgctgacc gaactcttcg gcaacccctg ccagctaaat
    18781 ctctgggtcc gcaccgcacc ccgcggggtc gagcacttcc actggcacct cgacctgatc
    18841 ccccggctcg aaccggcctc cgccttcgag atggctaccg gcgtggacat caacatcgtc
    18901 agtcccgaaa ctgccgccga gactctccgc gacgcgatcg actgagtagg ctcgtccgat
    18961 gccgatcgac aatcgcccct tcccccgttt cgttgccgat gcttcccgtc acggcattcc
    19021 tcacggccgc tttgccgagc ggctggctac cgctttcaag gacgcctgcc gcgagatcga
    19081 tgacctgccg gccggaacgg agatcccgga agagttcacc tggtttccgg agcgggcctg
    19141 gtcgggccgg gtctgggttc cggtttccgc cgacggcgaa gcggtcctcg aggaaaacag
    19201 cgaacccgag ccgatcgagt tcttcggctt cgtcagcttc gttcagccgg agggcggcga
    19261 tccgaccgac ttcaaggcct cggccgactt caccgacgtg gtcgccgccg acaaccccga
    19321 ctggacgatc gacatctccg atgaagtgat cggcacctgg cttggagagc agggccgcga
    19381 agcggcgatg accctggtct ggggccgggc cctggtccgt gacgccttcg cggtgactgc
    19441 cgagctcgac gatgtcaccg ttgaccagga tccccttttc ggcggccagg gcggtcgcgc
    19501 cgaccgcttt accctgatcg ctcccgacgc gctcaagaac tacggcgatg acgcctacct
    19561 cgagatcaag ctctggacct cccgcggcaa acaggtcgca cgggaatccc tctacgaaat
    19621 cgaagaaccg caaacagcac cggaacagga acccgccgaa tagccaccga atagccaccg
    19681 atggagcccg gtcatccatt tgtggatgtc gtgaccgggc gggttttact ggcattccgg
    19741 cgaagaccgg aatgcatgca ggtattttgg ttccatgaag aaccaaaagc gggttgagga
    19801 gcttcgggaa ctgatcgacc aggaatccga ggggccggtg ctttcggtct tctgccggac
    19861 cgaccctcgg gacccggcca acagcagcga gaccccggcc tgggcgatcg ccttgaagaa
    19921 cggccttaac cacgtggcgg gttttcacga gggaaaccac ggcaaggagc agacggtgaa
    19981 gaagctctgt gccgaagccg aacggcggat caacggggcc tcggccgccg accgcgggcg
    20041 cagcgtcgcg ctcttcatcg gcggtgacgg gaaggtcgac tcgttcacga ctttccagat
    20101 cccggttcgc gaggactatg tgacggtcga ctcgggcccg gtggtctggc cgatgctcga
    20161 tgtgatcgac cgcggccagc gcaccggcct ggtcctgctc tcgagtgacc ggatccggct
    20221 gctcgaatgg tgggacggcc gcacccggga tctcgaagaa tcgaccttcg acctcgaact
    20281 cggtgactgg caccagtacc ggggcccggc ccggccccag ggcacggtcg gtggtggcgg
    20341 cgtctcgaac gcctcggcct acgacgaccg ggtggaggtc tggcgggccc gcttcgtcaa
    20401 ggcggccgcc aaggcgatcg ccgaaagtgc cagcgagctc ggcttcgacc gcctcgtcgc
    20461 cgccgccgaa ggcgagctgg gggcaggctt caacgaggcc ctgcccgctg acatgcgaag
    20521 ccggctggcg accacggtcc acctcaatct gatcgacctt cccgccgccg aggccggggc
    20581 cgctcttgac ccgcatcttc gccaggcctg gcgcgacggg gtatccggtg acggggcgag
    20641 ggcaatggag cgctccaggg ctggcggccg tggcgccgcc ggtgccgatc gggtgctggc
    20701 cgccctcggc gaaggccggg tcgagcacct gatcctcgac ccttacctga agaccgacga
    20761 gctgaccctc gattcggcca gccggcaggc gatcgaggat gcgggcgaag aaagcgtcca
    20821 ggaggctctg gttgaactcg ccctccggac cggagcccgg gtcagctcgg cctcggtcga
    20881 cgaggtcccg gcattgaccg aagccggcgg cgtgctggcc ttgcttcgct attgagcggc
    20941 gcccaaggcc atcccggccc tccgccggga ccgaatcagg cctccacgga tagcatcccc
    21001 cttcggcccc ggtagctcag ctggatagag cgtccgcctc ctaagcggaa ggccgctggt
    21061 tcgaatccag ctcggggcgt gatcgctcgg gtgccacgcc gggcgaggca ggtgaggcaa
    21121 ccaaccagga ggattcgaaa tggcaaagat gaagcgcaag ccgatctcga cccgcaagcc
    21181 gccggtgccg gctgatgact ccactgacgt cgacgactgg acctcgcggg tgatgccgga
    21241 cctgcagccg atcgtcgcgt cactggatca gcagatccgc aaggcgatcc ccgacctgca
    21301 gtacgcggtg aagtggaaga aggcctacta cggtcgggaa gatctcggct gggtcatcga
    21361 gatggtcgcc tatgacatct cggtcaacat ggtctttctc ggcggtgccg acttcgattc
    21421 gccgccgccg ctcggggaca cggaccgcag ccgctacatc aagatcaagt cgctggaaga
    21481 agccgagaca ccggagatgg gagcctggat cgaacaggcc ggccgcgtcc cgggctggaa
    21541 gtagccgggg ccgtaggatc gccccatgcc gttcacgcgc atcaacctca agcaggacgt
    21601 ggacaacttc gggcccaact tcgacggctc gccggacctc gatttccggg ccgcgacgaa
    21661 gccgctcgaa ctcgagcatt cattcctggg ttatcagcgt attccgccgg gatacagatt
    21721 tcccttcggc cacacccacg acagccagga agaggtctac gtggtcgtcg gcggcagcgg
    21781 caggatgaag ctcgatgacg agatcatcga acttgcgaaa tgggatgcgg tgagggtgcc
    21841 gccaggcacc tggcgaggct atgaggctgg accggatggc ctcgagatcc tgatttccgg
    21901 cgctccgaac ctcggcgatc aggttcgtga cgacgtgacc ggccagcgcg actggtggca
    21961 ggactgagat cgccttggct gactgggcgg atgacttctt cgttgagtgg cagagggcct
    22021 gggggaccgg gaccgaggcg gtgctcgagt tcgtcaccga tgacatcgag tactgggacg
    22081 tgaccctgcc cgagccgatt cggggccgtg aggcctatgc ggggcattgc gacaacttct
    22141 tcaccgcctt cactgaatgc gagtggtcac tgcgcgaacc ggtgatctgc gagggcgaca
    22201 gggtgaccga gccctgggcc ttcaccgggc tcaattcggg tccgatctgg atcggcctgc
    22261 ctgccaccgg ccgccggctg gaatcgaagg gcaccgacat cttcgaattc cgggacggca
    22321 aggtttgccg cgagcattcc ttctacgacg tggtcggcaa tctccggtcc ctcggcctct
    22381 ggcccggcat cggcagccgg accgaagccg cggcaatgac cgcggcgggg gctgcgatcc
    22441 gcggcaccgg catggtccgg aacctccgcc cttgaccccg cggcaccggg ctggtccgac
    22501 acctccgccc ttgagacccc gacaccgcga atgcccactt tgcgccacat accggcacaa
    22561 agtgggcact cggtgacccc ggcccccggc tagcggggcg catatgatcg gcccgacccc
    22621 cgaggaggag aaatgaccga gatcgatcgt ggccgcctgg ccgagctcac ccagtccgag
    22681 ctcgaccgtt tcgtcgagga aaacccgaag tcgaaggagc ttttcgagcg ggccaggaag
    22741 tctctggtcg gtggcgtccc gatgccctgg atgatgcgct gggctggcgg tttcccggtc
    22801 ttcgcgaagc tggcgatcgg ggcgcggatc atcgatgtgg acgaccacac ctacatcgac
    22861 ttctgcctcg gagacacggg tgcgatgccc ggccatgatc ccgagccggt ggtgcgggcg
    22921 atcaccgatc aggagcgcaa gggcatcacg acgatgctgc cgaccgagga cgcgccctgg
    22981 gtcggggagg aactgacccg ccgcttcggt ctcgaccgct ggatgttcac cctgaccgcg
    23041 accgatgcca accgggcggc gctgcgaatc tgccggcaga tcaccggcaa gcccaaggtt
    23101 ctggtcttca gctacagcta ccacggctcg gtcgatgagg ccttcgccgt ggccgggccg
    23161 gaaggcgtca cggtttcgcg ggaaggcaac gtcggagccc cggtcgaccc ggcgcaaacg
    23221 actttcgcgg tcgagttcaa tgatctggag gcgctggagg ccgccctggc gacgggcgag
    23281 gtcgcctgcg tgctggctga accggcgatg accaacatgg gaatcgtcct gccggacgag
    23341 gggtaccacg aggcgctgag gaacctgacc cgaaagcacg gcgccctgct gatcatcgac
    23401 gagacccaca ccttctcggc cggcccgggc ggctgcaccg cggcctgggg cctggaaccg
    23461 gacctgctct cgatcggcaa ggcgatcggt tccggcgtgc cggccggggc cctcggcatg
    23521 accgaagcgg tgaccgaaag cctctttgcc cagcaggacg ccgattacga ggacaccggc
    23581 ggggtcggcg gcaccctggc cggcaatgcg ctctcgcttg ccgcggtccg ggcgaccctc
    23641 tcggaggtgc tgaccgaaga ggccttcgcc cacacggttc ccctcgccac ccgcttcagc
    23701 gaaggtgtga tgcaggtggt caccgacgcc gggttgccct ggaacgtgac ccagctcggg
    23761 gcccgggccg agtaccgctt tgagccggaa ccggcccgca acggcaccca ggcccacaac
    23821 gcttccgacc cggaactgga gcgttacctg cacctgcacg cgctgaaccg cggcgtgctg
    23881 atcaccccct tccacaacat ggcactgatg tcaccggcca ccatccccgc cgacgtagac
    23941 cgccacaccg aagtcttcaa agaagcagtt ctcgatctag tcgggtagtc accagtcaaa
    24001 ccggtgctgc agggcggtgc ggggtgtgcg cccaaaacct ccggaatgag gagcgaagcg
    24061 acggttttgg gggtacaccc cgcaccgccc ccaaccacgc ggctataggc tgcgcaacgt
    24121 ggaagcctcg atggagaaca aaccgaccgt cgcaatcatc accggagcct caagcggaat
    24181 cggcgaaggg gtcgcctggc gcctggcccg caagcccggc acccgactga tcctggtcgc
    24241 ccggcgtgag gatcgcctgc gggaactggc caaacgcctg tcctgcgaag tgacctggat
    24301 cgcggccgac ct
//
LOCUS       DAOQKN010000014        22663 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_972_length_22663_cov_6.137695, whole genome shotgun sequence.
ACCESSION   DAOQKN010000014 DAOQKN010000000
VERSION     DAOQKN010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 22663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22663
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_972_length_22663_cov_6.137695"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PLJ64_01940"
     CDS             <1..192
                     /locus_tag="PLJ64_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92286.1"
                     /translation="PEEPAEPLALMTLVALLLPIVVIPLVGHAISNDAGDIAFGALAL
                     FLVVATLANLKRSGIPLTR"
     gene            220..2010
                     /locus_tag="PLJ64_01945"
     CDS             220..2010
                     /locus_tag="PLJ64_01945"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNC92287.1"
                     /translation="MEPSASSEVAAKKIEPGTPGDGLILNPKSWSAPHLDAADQERLK
                     SMIQWFENRGLKALKDDDRENRWYSDFLEMQAELGIFCNFLTPARDGEGDPARRWDTT
                     RICEFNELSGFYGLPYWYTWQVSILGLGPIFQSENEAARKKAADELARGSIFAFGLSE
                     RAHGADIYSTDMVLTPAGDDGAGETYTATGGKYYIGNGNEAAMVSVFGRVDGIEGPDG
                     YAFFAASSSHSNYELVRNVVNSSNYVSQFNLNAYPVESADLLHQGPGAFDAALNTVNI
                     GKFNLGFASIGICEHAFWEAITHADSRVLYGMKVTDFPQVRQAFTDAFARTAAMKLFA
                     WRAVDYFRTASPEDRRYLLFNPLTKMKVTTEGEKVVDLLWDVIAAKGFERDMWFEMAA
                     RDIRALPKLEGTVHVNMALVLKFMASYLFNPDPELAAPERRQDPGDDEFLFRQGPARG
                     LGKIRFSPWRESYEPFASVPNVAKFMELAESFKAFLVEAGPDEAQQKDLDFLLTLGEI
                     FTLIPYGQLICEQAALIGLPEDTLDQIFDVMIRDFSAYAAELHGKTSSTEAQQEWARG
                     AITKPVVDQARFDSVWEKTRALAGGYEMNP"
     gene            complement(2007..2174)
                     /locus_tag="PLJ64_01950"
     CDS             complement(2007..2174)
                     /locus_tag="PLJ64_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92288.1"
                     /translation="MRQAAWLFDIQIAAVAFAAGLDVATRNIRDFEELSASLERLYPG
                     APTLAVYPDFR"
     gene            complement(2502..2870)
                     /locus_tag="PLJ64_01955"
     CDS             complement(2502..2870)
                     /locus_tag="PLJ64_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92289.1"
                     /translation="MTKREPKGESGAEARRQALKSGGAAVVTHRKPRAPRSDGPRTTL
                     RVPDSLVISAERLAGELGISRNDALLRLATRGADLYETERLIEKRREERWKAVVPGEI
                     EPGVSFPEAEEIAEAVRPGL"
     gene            2927..3352
                     /locus_tag="PLJ64_01960"
     CDS             2927..3352
                     /locus_tag="PLJ64_01960"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNC92290.1"
                     /translation="MQRMRFDKDFDLPPERVFDFIAEHENLGGVLGAEVTRLSYGTDG
                     NRNGVGSSRKLNPPGPVPAFEETVTEFEPHSRIVYTVTKGTPLNHHLGTITFAPTDSG
                     THMEWNIEIGTRLPGLDFVIAKVLVRNIGGGLDRMTIPA"
     gene            complement(3362..4030)
                     /locus_tag="PLJ64_01965"
     CDS             complement(3362..4030)
                     /locus_tag="PLJ64_01965"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNC92291.1"
                     /translation="MNTSIAEDSVSPEELTEAVSERRGWKLYQVIRLILRPLRWWARV
                     ESKGLEYLPATGPALIVSNHDSWLDPLAIIEVMMWKKRQLRFLAKHTLWKFRPMAWIL
                     DQAAQIPIRRGEGDTGALDAAAVAVERGEMIGIFPEGTLSRGTVLRARKGVSRLARAC
                     PGVPVVLVAVTGSSDLKRFPKRPRVKVEFFDPAGGQLRPDEDAQEFSQRLLDEIRAKA
                     PRVK"
     gene            complement(4312..5121)
                     /locus_tag="PLJ64_01970"
     CDS             complement(4312..5121)
                     /locus_tag="PLJ64_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92292.1"
                     /translation="MRAASVRENSRLRNLLREIGEIFAGIFHVYRAWWVDILVLSFCI
                     FVPIGLLDAADNQALESIGPGHDLTATALGIAALVITATALLGEVFLAGAVGLSLIHA
                     EDGRPPSLRFIARHLSYGRLIAVDILFVLAVALGLVFLVVPGMAAFAYLALAGPVVEI
                     EDRGIASSFRRSFQLVRGRFWLVFLILFPIEAVGGAVGQGSAAICETLFGHTFFAEGI
                     AEALADIAISPLFAIASVLLARKLIMARDGIELPGPGLRDLQPAPSGKPPA"
     gene            complement(5178..5999)
                     /locus_tag="PLJ64_01975"
     CDS             complement(5178..5999)
                     /locus_tag="PLJ64_01975"
                     /inference="COORDINATES: protein motif:HMM:NF037026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92293.1"
                     /translation="MDQSGVAEAVAWAESEGWQPGLGDHLAFFAADPEGFFHSRADGQ
                     TVATISVVRGSPEIAFVGLYIVAAEFRGRGFGRQLWDEALGKFDGFTLGLDAVPEQEA
                     TYASDGFASAYGNARFSAEARELPDPEPDAGILPSSSVPFDQLVAFDAAHYFGPRPAF
                     LRAWIKGEGRQSLVATDGTSITGFVASRPTRTGNRIGPFFAANATIAGNLLLELATRL
                     TGPVSIDAPEPNQAASDLFTSLGMKRSFPTIRMYRGLPPDLPLDRIFGITTLELG"
     gene            complement(6017..6619)
                     /locus_tag="PLJ64_01980"
     CDS             complement(6017..6619)
                     /locus_tag="PLJ64_01980"
                     /inference="COORDINATES: protein motif:HMM:NF013918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HNC92294.1"
                     /translation="MGNGIHIFWVLSRGAGIAAILFAGLSVTVGLLSSRGMPFPNLRK
                     NFELRPLHEALSMATILLVAAHGLLLLGDPWLKPGITGISVPFVMSYRSFWTGIGIIA
                     GYGLILLGLSYYLRRRIGPSRWRTAHRFIAIFWLIGLAHTFGAGTDASQPWVWVPVAL
                     TSAPALVLLIMRLSRQGSKPAPARVAGTPVSDRAVMIDRH"
     gene            complement(6612..7529)
                     /locus_tag="PLJ64_01985"
     CDS             complement(6612..7529)
                     /locus_tag="PLJ64_01985"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNC92295.1"
                     /translation="MNDLATTLCFPALGGEVLIQAEGPGSDLAVEEARELVLDLHRRL
                     TRFEPSSELCQLNRSSDRAVKASPLLLRFADEVHRAGSNSGGLVDATLLDQVEAAGYR
                     DSINPDDPAGPGPSSPELEPPARRWSWSDVTVDREDSTVIRPPGLRLDSGGIGKGLAA
                     DIVAERLEGFDSWAVECAGDLRFGGEGGAERRIDVKSPFPGRGVIADFTASAGAVATS
                     GTTRRSWKTGSGDSHHLIDPRTGRPARTGLVQVTAVAPTAVEAEVRAKAALLSGPDRA
                     SEWLIDGGMFVTEEGEIRAWDFQRRPEVG"
     gene            complement(7526..7888)
                     /locus_tag="PLJ64_01990"
     CDS             complement(7526..7888)
                     /locus_tag="PLJ64_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92296.1"
                     /translation="MNELTPKEMAEAKKLARRVRVGNIRRRIAYGATLMVAVFSGAIL
                     FRSLEQQTGTAATNVSAVTRSDESDDSEGLSALVTQVSSAFGDDGEEEEGSPSTTAGT
                     VTGSTTTSSAPPLVTSQS"
     gene            complement(7903..8310)
                     /locus_tag="PLJ64_01995"
     CDS             complement(7903..8310)
                     /locus_tag="PLJ64_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92297.1"
                     /translation="MSETHDDQKPDQNDPATTELTPEKGWSATTRRLADNPVGRWVAV
                     GAAVVVALFSGMLIGRATGQDPAEAGPASFGRPDGDGPPAGGQVPFHGDDDFGGQSYG
                     DDDSYEQPEGGDDEHSESSSGGSSPSPLETGQS"
     gene            8413..9087
                     /locus_tag="PLJ64_02000"
     CDS             8413..9087
                     /locus_tag="PLJ64_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC92298.1"
                     /translation="MRILLVEDEKAIADFIARGLEAEGHAVTWVADGIDGEALARTGR
                     FDLILLDLMLPGKDGLEVLASLRREDGSTPVILLTAKGEVADRVAGLDAGANDYVTKP
                     FAFEELVARIRAQLRSPAQGSATELRTGDIELNLLRRRVKRGDVEVLLSAREFDLLAY
                     MMRHPGQVLSREQLLNGVWGYDYDPGTNIVGVYIAYLRRKLAMEGRPDPIETLRSVGY
                     RLRDGR"
     gene            9090..10562
                     /locus_tag="PLJ64_02005"
     CDS             9090..10562
                     /locus_tag="PLJ64_02005"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNC92299.1"
                     /translation="MPHPGLRGKLAISIALVLLIALGVTYFAVYRGTGSELKDRTESE
                     LDKGVSQIAARLKVGGADTPAEHTARAHQITSAEGFGPNARLVSISIEGGGTASNQPE
                     LVGPGLEDHHSDGEQKHAGHESGDDSEDEPAEKKQDADRILGSAPGYSTIEVEDAGKV
                     RILTREVELPGGVKATIRVGQPLAPAENALEGLSDTFVIVGIATLLLGALAGWLLAST
                     ATRPMRRMAQVAEGVDAGDLSARMPVEGTRDEARRLAESFNGMLDRLEDAFDRQRAFV
                     ADASHDLRTPLTVVRGQAEVLARNPNPSREEVTRVYELVSQATARMELMVDDLLLLAR
                     AESSEGLRLEPLELEPLISAEVEGMAFENNRDLVAGVVTGRRVNIDREQVARLLSNLI
                     SNAIAHTEEGGRIEVSATDHGSKVVLAVDDDGPGVPESDRERVFDRFARLDESRSSSL
                     GGSGLGLAIVRAIAEAHGGEARCVDSPLGGARFEIEFPAA"
     gene            10600..11769
                     /locus_tag="PLJ64_02010"
     CDS             10600..11769
                     /locus_tag="PLJ64_02010"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF008398.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HNC92300.1"
                     /translation="MRFSADQPLNVQPATVRDYRELARRRLPRQLFDYIDGGAYEEVT
                     ARANETDLAKVLLRQRVMRDVSVREQATEVLGQKLSMPVILGPVGLAGMFATRAEVQA
                     ARAAEAAGVPFTESTVSICSIEEVAEATSVPPWFQLYVMRDRGYAEKLMARAEAVGAP
                     VLALTIDLAVVGARHRDTRNGMGDSPTRVAQLRRGLDLVSHPGWVRRVPIAGKPLTFG
                     NLAEVVPGARTPEDFKEWVDAQFDPSVTWDDIAWVRENWKGKLVVKGVLDPEDARKAV
                     DAGVDGVAVSNHGGRQLDAVPSTAAALPGVAEAVGDETTVLADGGIRNGLDMVKMLAL
                     GAEAVIIGRAWAWAVAAEGQAGVEKMLRVLKSDADVALALTGQNSVAGLGPDSLV"
     gene            complement(11777..14002)
                     /locus_tag="PLJ64_02015"
     CDS             complement(11777..14002)
                     /locus_tag="PLJ64_02015"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNC92301.1"
                     /translation="MPGIRFRGILVSAAFAVLPVFAAPAVSSAGDDPGTSVLEVRFSR
                     SASLRGDEASLRAASEPALDRVEKLFERIGTISAEPLAAGVSQAESTALARRASRLSG
                     AMTTNMADWYRVEVPAGTEADALDALRNSGQVAYAGRAPVALPPPVTPDFSPGQLHLD
                     MAPTGIGAEYEETEPRVRGGGVTVVDLEYYWTRTHEDLQLPASSDIGGGEYVQYTAFD
                     DEHGTAVFGILNADDNGFGVTGIVPEATMKGISPTMAPDFGYNPAGALTYLADKLSPG
                     DVVLIEQQADGPGPGPSDYVPMEWNQASFDAIRQLGDLGIVVVETGGNGGYDLDSPAM
                     LGRFNRANRDSEAIVVGAGDSVTHAPLWFTSHGSRIDLQGYGNNLVTTGSNGDLQGPG
                     PGEKDIRYTSSFDGTSGAGPVVAGAVAAVLSYLKATGQPPLDADQLVGLLRSTGTPQA
                     SPELGQIGPLPDIPAAIATIQGSLPTVSITGPGDGLTFDFNSAHFLGLTCGTGAGPDL
                     ESCLARDQGPDGETNLVTGDRLPTDQPGTHTLTATATNELGLTATDTVTYEVGPGCFA
                     DGVRLASVQPRGAKVKLLGAADPSSAGRQARILRAGKRAGTATVTGDGRIEATVSAPK
                     SKRLRPGTRYRLVVGGSRSNPMKPSGAVRVLSRKPLPDADLIKGKLAGVRKQGRFTLR
                     ARPLCGGPATSRQVSFNRSGVFSVRIGFTSATRTFSIRRSGGEVPLPVVMPAARFITG
                     N"
     gene            complement(14234..14596)
                     /locus_tag="PLJ64_02020"
     CDS             complement(14234..14596)
                     /locus_tag="PLJ64_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92302.1"
                     /translation="MRRAAAVALALTGVIHLVLAPEYMAEQAYIGLLFIGGGITTIAL
                     AAWLWVRSEPVAWVGAALVSAGMAAGFILSRTVGLPGFHEAEWELSGVVSVLLEIGVV
                     GLAAAALGSGGSARTATS"
     gene            complement(14707..15723)
                     /locus_tag="PLJ64_02025"
     CDS             complement(14707..15723)
                     /locus_tag="PLJ64_02025"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HNC92303.1"
                     /translation="MSTENRRYALAERPEGLIDEDTFTLETGPVPEIGEGEALVRVTW
                     ISIDPTNRTWIGEEPTYLPPVQIGETMRALGLGEVVESNNPNYPVGALVTGLTGWTDY
                     TVISDSNPLMVVPEDARSAPPAALLGMLGMTGCTAYFGMLEIGEPKEGETVVVSAAAG
                     AVGTVAGQLARLKGARVVGIAGGPEKCAWLIDELGFDAAVDYKADDWKEQLRAATPDG
                     IDVNFENVGGEIMEAVLLRLNINARVALCGLISGYNDDLRPLDPRIFGRLLVNRVRLQ
                     GFIILDYYARFGEAIRELSGWVAEGKLKSEQTVVEGFENLPDALNMLFKGENKGKLVV
                     SIAK"
     gene            15765..17309
                     /locus_tag="PLJ64_02030"
     CDS             15765..17309
                     /locus_tag="PLJ64_02030"
                     /inference="COORDINATES: protein motif:HMM:NF015043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HNC92304.1"
                     /translation="MGKTRRDLLVDGTKVAAGLAAANALAPTGPFGAAQALADPKRRL
                     RRPGFGLAFKSLKDETFLPSIDLDGKLPQWLTGSLLRNGPALFEIGEEKFNHWFDGLA
                     MLHAFSFKGGKVSYRNRFLRSSQYWAWQREGRIKFSELATDPAPDPCRQIFSGVSTLP
                     VLGRIPNANVSIEKLAGEFRAHTEIPIPVRFNPKFLNTMGIGDAGQMQGRLGTAHPHF
                     DPETRERFSYEVELVPPSGLRIVSEKGRTRRELAFIPQAMPGYLHSFGLTRRFVVIVS
                     QPFNFNLSKFLSPDRGPIITNYEWQGEEPTRIILVDRQRGGVAHTVETDSFFAFHHIN
                     AYEHEGKVIVDVCAHKDAGVIDALYLKKIRSGSKVPQARYRRFEIRLEGQGKLSQRDL
                     IDAYIELPQKNYGRVNGRHYRYAYGVGLRKGSSGFIDQLVKADVKIGESKLWREWGVY
                     PGEPVFVPRPGGKAEDDGVILSVILDGRRRKSALLVLDARNMEEIARASVPHHIPFGF
                     HGLYAS"
     gene            17415..18923
                     /locus_tag="PLJ64_02035"
     CDS             17415..18923
                     /locus_tag="PLJ64_02035"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HNC92305.1"
                     /translation="MIGLTALVLVVPCAAAIVLRATGIVTSPVLLILIPVLVSVLFSH
                     SISEFWKRRRAGSPLLFEDLMLWGWLRRRRFERLLSRSEDFVGPEAGRGLTPKQRARE
                     LERLASALEARDPRTHGHSRRVARHATSIARGLRLPDAEVARIRTAALLHDVGKIEVP
                     WEILEKPGALTDEEFAQIKKHSAAGARLVEGMGDPELVAIVRHHHERIDGGGYPDRIA
                     GDEIPIGARIIAVADTFDALTSARSYRQPRSHEEALGVLREEAGAQLDAGAVQAFDGR
                     YSSRRPVALVATVLGLGRQAGQSMISFGTGASQVAAVGAAAAVIGVAPAARVDQQQEP
                     AKQNPIVRQSDSTSARSVADSGSATAGSQSGTGEQGRKSASGGRNARNGNGGKGSTDN
                     TFSTSGDGGGTSSGSNGGSGSGGGSSGGSGGGSGGGSSSGGSSGSGTTAVNPVQTVTE
                     NVQKQVQQTTQQVTDSIPSLPGNDPVSQGVNNTVDEVKDTVNSTVGKLTGKP"
     gene            complement(18933..20003)
                     /locus_tag="PLJ64_02040"
     CDS             complement(18933..20003)
                     /locus_tag="PLJ64_02040"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNC92306.1"
                     /translation="MAEAGENVELAPLTTLKLGGPAERLIKVEDTAELIRLVQAADAE
                     REPLFFLAGGSNVVIADQGVPGTTVWIRTAGIDERELDDGRVSLTVAAGENWDALVAR
                     TVDSGLSGIECLSGIPGTVGATPIQNVGAYGQEVSSTIASVLVLDRENAEVVTLAGSE
                     CGFGYRSSRFKGDSRFLVLAVEFILSPSEMSAPVEYAQLAGALEVEVGERVPLPVARE
                     TVLELRRSKSMVVDPADPDSVSAGSFFTNPLLDLASMADLTARAEELFGADDLPPGYP
                     APEGLAKTSAAWLIERSGFTRGFGDGPIGLSRNHTLAIVNRGGGTTAELVAFAHGIAD
                     RVEEIFGVALVPEPVFVGHAWR"
     gene            complement(20099..20833)
                     /locus_tag="PLJ64_02045"
     CDS             complement(20099..20833)
                     /locus_tag="PLJ64_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92307.1"
                     /translation="MKIRRRERFGILAAAILALFVIVMPGTASAKDHNKDRIPDRWER
                     SHKLSLKRDQRKLDQDTDGLRNRGEWLAGTNPRDKDSDDDGVNDLKEKAGVISAYDSE
                     TGDLTITLYAGGELTGSVTDETRVHCESEDVADDNGTDEGDDNGTDEGDDDGTDDQGS
                     GESEDRHGGPGSNGEGHDDHGNGEGRGHDGEGPGHEGDCGGDCSGDDLAEGVEVTEAV
                     VKYTASGKVFKELEIVVPAGDTSTQA"
     gene            complement(20953..22461)
                     /locus_tag="PLJ64_02050"
     CDS             complement(20953..22461)
                     /locus_tag="PLJ64_02050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNC92308.1"
                     /translation="MAAENPGSIRPLARGTLKLQSDERLLKLFRRGVEPAFEELINRY
                     RVTLVAYAGSIAGPDRGEDVVQDALVKAHRSLRDDRDIDPRPWLYAVVRNTALNDVRD
                     NRKHRHDGLGETATGGTSPDELVEQRERFAAVLAAVADLPPAQRRALVDHELSGFSHE
                     EIAAELDLSTGATKQLIYRARLSLRNAFGAMIPIPLIAWLAEGSGVVATGAAGTGATA
                     TGLMGAIGGGSAAKLAVVAVVAGGSLAGGIAVERHQADRGTHPDTAQAAEPGDSGAAA
                     PNGEDSGGDDGPGNGSLVADRSRTGSDKGSDGAEDRGESDRGSQGGRQTGEGGRDSGD
                     SRPGGSGSSGPGSGGSGGDDHPLGVRPAGGRHEPGEDSDEDSGNGGHGDDRPSRGVSG
                     GGPGPSGGPPREDDSSGHGPSGGSDESDDSDWHPGSGGSGGSGGSGGSGGSSGSSGGS
                     SGGSSGSSGGSSGGSSGSSGSGGSHGSDDSGSGSSGSSGGEGGRTPHPSDDD"
BASE COUNT         3843 a         7518 c         7535 g         3767 t
ORIGIN      
        1 cccgaggagc cggccgagcc gctcgccctg atgaccctgg tcgccctgct cttgccgatc
       61 gtggtcatcc cgctggtcgg ccacgcgatc tcgaatgacg ccggtgacat cgccttcggc
      121 gcgctcgccc tgttcctcgt cgtggcgacg ctcgccaatc tgaagcgatc cggcatccct
      181 ctgaccaggt gaaccctgat ccgggcgtat gctcccggca tggagcccag cgccagttct
      241 gaagttgccg ccaagaagat cgagcccgga acccccggtg acggactgat cctcaacccg
      301 aagagctggt cggcgccaca tctggatgcc gccgaccagg agcggctgaa gtcgatgatc
      361 cagtggttcg agaaccgtgg cctcaaagct ctgaaggacg acgaccgcga gaaccgctgg
      421 tactcggact tcctcgaaat gcaggccgag ctggggatct tctgcaactt cctcactccg
      481 gcccgggacg gtgaaggcga cccggccagg cgctgggaca cgacccggat ctgcgaattc
      541 aacgagcttt ccggtttcta cgggctgccc tactggtaca cgtggcaggt ctcgatcctc
      601 ggcctcggtc cgatcttcca gtccgaaaac gaggccgcga ggaagaaggc ggccgacgag
      661 ctggccaggg gctcgatctt cgccttcggc ctctccgagc gggcccacgg tgccgacatc
      721 tattcgaccg acatggtcct taccccggcc ggtgacgatg gcgcagggga gacctacacc
      781 gcgaccggcg gcaagtacta catcggcaac ggcaacgaag cggcgatggt ctcggtcttc
      841 ggtcgggtcg acgggatcga aggcccggac ggttacgcct tcttcgcggc cagctccagc
      901 cactcgaact acgaactggt ccgcaacgtg gtcaattcat ccaactacgt ctcccagttc
      961 aacctgaacg cctatccggt cgagtcggcc gacctgcttc accagggccc gggcgccttc
     1021 gacgcggcgc tcaacacggt gaacatcggc aagttcaacc tcggcttcgc ctcgatcggg
     1081 atctgcgaac acgccttctg ggaggcgatc acccacgccg acagccgggt tctctacggg
     1141 atgaaggtga ccgacttccc ccaggtccgg caggccttca ccgacgcctt cgcccggact
     1201 gccgcgatga aactcttcgc ctggcgggcg gtggactact tccgtaccgc ttcccccgag
     1261 gatcgtcgct acctgctctt caacccactg accaagatga aggtcacgac cgagggcgag
     1321 aaggtggtcg acctgctctg ggatgtgatc gcggccaagg ggttcgaacg ggacatgtgg
     1381 ttcgagatgg ccgcccgcga catccgcgcg ctgccgaagc tggagggcac ggtccacgtg
     1441 aacatggctc tcgtcctcaa gttcatggcc tcctacctgt tcaatccgga ccccgaactc
     1501 gccgcaccgg agcgccgcca ggatccgggt gatgacgagt tcctcttccg ccagggcccc
     1561 gcccgcggcc tcgggaagat tcgcttcagt ccctggcggg agtcctacga acccttcgcc
     1621 tcggtgccga acgtggcgaa gttcatggag ttggccgaat cgttcaaggc attcctggtc
     1681 gaggcggggc cggacgaggc ccagcagaag gacctcgatt tcctgctcac cctcggcgag
     1741 atattcaccc tgatcccgta cggccagctg atctgcgaac aggcggcgct gatcggcctg
     1801 cccgaggaca cgctcgacca gatcttcgac gtgatgatcc gcgacttctc ggcctacgcg
     1861 gccgagctcc acggcaagac ctcctcgacc gaggcccagc aggagtgggc ccgtggcgcg
     1921 atcaccaagc cggtcgtcga tcaggccagg ttcgactcgg tctgggaaaa gacccgcgcc
     1981 ctggccggcg gctacgagat gaacccctag cgaaagtcgg ggtagacagc cagcgtcggt
     2041 gcgccggggt agagccgctc aagcgacgcg ctcagttcct cgaaatcacg aatgttccgg
     2101 gtagcgacgt ccagcccggc agcgaaagcg accgcggcga tctgaatgtc gaacagccag
     2161 gctgcctgtc tcatcgtttt ggaccttgaa tcacggcccg gaggggggtg gggattctcg
     2221 gcccgaagtc gaccggcaat aacggcggcc cgcccgtcca gtgaaatcac gttcaacacc
     2281 tgggagccga tcaatcgaac cagccagtcc cgcaggacac cgaatcgacg atcgcctgac
     2341 atggccttca actcgatccc gtagaacatc tcagtgagcg agggtgctgc gatcggtacc
     2401 ggtctaccct ggcgtgattc ggagaccgcg tagtcgaaaa ggctgctatc cgggcggata
     2461 cgagaagcga atgtggtgtc ccacaagcgg tccaccaaaa ctcaaagccc gggacggacc
     2521 gcttcggcaa tttcctctgc ctcggggaag ctgactcccg gctctatttc acctggaacc
     2581 accgctttcc accgctcctc acgccgcttt tcgataagcc tttctgtttc gtacaggtca
     2641 gctccccggg tggcgagccg gagcagggca tcattgcggg aaatgccgag ttcgccggcg
     2701 agccgttcgg ctgaaataac caacgagtca ggaacccgca aagtagtacg cggaccatcg
     2761 gaacgcggcg ctcttggctt gcggtgggtg accacggcag cgccaccgct tttcagcgcc
     2821 tgccgacggg cctccgctcc ggattcgcct ttcggttcac gtttcgtcat gtctcgatca
     2881 taacgcatca gttgatgcgc taccgcgaat ctgattcaat ccggccatgc agcgcatgcg
     2941 atttgacaag gacttcgacc ttccgcccga gcgggtcttc gacttcattg ccgagcacga
     3001 gaacctgggc ggggtcctcg gagccgaggt aacccggctt tcctacggca ccgacggcaa
     3061 ccgcaacgga gtcggttcct cgcggaaact gaatccgccc gggccggttc cggccttcga
     3121 ggagactgtg accgaattcg agccgcacag ccggatcgtc tacacggtga ccaagggcac
     3181 cccgctcaac catcacctcg gcacgatcac tttcgccccg accgattccg gaacccacat
     3241 ggaatggaac atcgagatcg gcacccgcct gcccggcctc gacttcgtga tcgccaaggt
     3301 gctggtcagg aacatcggcg gcggactcga ccggatgacg atcccggcct gaaccccggc
     3361 gctacttgac gcgaggcgcc ttggcgcgga tctcgtcgag caggcgctgc gagaactcct
     3421 gcgcgtcctc atccggtctc agctgccccc cggccgggtc gaagaactcg accttcaccc
     3481 ggggccgctt ggggaaacgc ttcaggtcac tgctgccggt caccgcgacc agcacgaccg
     3541 ggactcccgg gcaggcccgg gccagccggg agactccctt gcgggctcgc agcaccgtgc
     3601 cccgtgaaag cgttccttcg gggaagatgc cgatcatctc accgcgttcg acggcaactg
     3661 ctgcggcgtc cagggcgccg gtgtcaccct cgccgcgccg gatcgggatc tgcgcggcct
     3721 gatccaggat ccaggccatc ggcctgaact tccagagcgt gtgcttggcg aggaaacgca
     3781 gttgacgctt cttccacatc atcacctcga tgatcgccag cgggtcaagc caggaatcgt
     3841 ggttcgagac gatcagcgcg ggaccggtcg ccggcaggta ctcgagcccc ttcgattcca
     3901 cccgtgccca ccagcgcaac ggccgcagaa tcaggcggat gacctggtag agcttccagc
     3961 cgcgtcgctc ggaaacggcc tcggtcagct cttctggcga gaccgaatcc tcggcgatcg
     4021 atgtgttcat cggcttcccg ccgtgccttg cggattcatg aacatcggac tctagagcct
     4081 gagactctga agactgcggt gttcgcggaa atcacccgaa acgggcggtg aacaggtcgc
     4141 ggcggcactc gccggatcga tccgaaaggc cggtgcgaag gctccggcag cagccagcgc
     4201 cgcacctcgc ccgagtagcg cgcgtctggt cagcggtcgc ccgctcacgg cctccccttt
     4261 ctcccgctcc acccgcggca gtctagcttg cggtcccggt gctgctgaag atcaggccgg
     4321 gggcttcccg gacggggcgg gctggaggtc ccggaggccc ggaccgggaa gttcgatgcc
     4381 atccctggcc atgatcagct ttctggccag gagaaccgaa gcgatcgcga acagtggcga
     4441 aatcgcgatg tcggccagcg cctcggcgat gccttcggca aagaaggtgt ggccgaagag
     4501 cgtctcgcag atcgcggcag agccctgccc gacggcgccg ccgaccgcct cgatcggaaa
     4561 gaggatcagg aagacgagcc agaagcgtcc gcggacgagc tggaagctgc gtctgaacga
     4621 gctcgcgatg ccacggtcct cgatttcgac caccggcccg gccagcgcga ggtaggcgaa
     4681 ggcggccatg ccgggaacca ccagaaagac cagcccgagg gccaccgcca gcacgaagag
     4741 gatgtcgacg gcgatcagcc ggccgtagct gaggtggcgg gcgatgaagc gaagcgacgg
     4801 cggccggccg tcttccgcat ggatcagcga gagcccgacg gcaccggcga gaaagacctc
     4861 gccgagcagg gccgtagcgg tgatgaccag ggccgcgatt cccagggccg tcgccgtcag
     4921 gtcgtgaccc gggccgatcg attcgagcgc ctggttgtcg gctgcgtcaa gcagtccgat
     4981 cgggacgaaa atgcagaacg aaagcaccag gatgtccacc caccaggccc ggtagacatg
     5041 aaagatcccg gcaaagatct ccccgatctc acgaagcagg ttccgcaagc ggctgttctc
     5101 cctgactgag gcggcgcgca tcgtgagaag tctctcaatc acgttggccc cgtgcgatcg
     5161 gaccgggtcg gaagggatca gccgagttcg agggtggtga tgccgaagat tcggtctaga
     5221 ggaaggtcgg ggggcaggcc gcggtacatg cggatggtgg ggaagctccg tttcatcccc
     5281 aggctggtga agagatcgga agcggcctga ttgggttcgg gggcgtcgat cgagaccgga
     5341 ccggtgagcc tggtcgcgag ttcaagcagc agatttccgg cgatcgttgc gttggcggca
     5401 aagaaggggc cgatccggtt gccggtgcgg gtcgggcggg aggcgacgaa gccggtgatc
     5461 gaggtgccgt cggttgcgac aaggctctgg cggccctctc ccttgatcca tgcgcgaagg
     5521 aaggccggtc tggggccgaa gtaatgagcg gcgtcaaagg cgaccagctg gtcaaaggga
     5581 accgaggagg aaggcaagat gccggcgtca ggttccggat cgggaagctc gcgagcctcc
     5641 gccgagaacc tggcgttgcc atacgccgaa gcgaaaccgt ccgaggcgta ggtcgcttcc
     5701 tgttccggga ccgcgtcgag gcccagcgtg aaaccatcga actttcccag ggcctcatcc
     5761 caaagctgcc tgccgaaacc gcggccgcgg aactcggcgg cgacgatgta aagcccgacg
     5821 aaggcaatct ccggcgatcc ccggaccacc gagatcgtgg cgaccgtctg cccgtctgcc
     5881 cgcgagtgaa agaagccttc cgggtcagcc gcgaagaacg ccaggtggtc accgagaccg
     5941 ggctgccagc cctcgctttc tgcccaggcg actgcctcgg caacccctga ttgatccatc
     6001 gctttgatgt gaggattcaa tgccggtcaa tcatgaccgc tcggtcggaa accggggtgc
     6061 cggcaacccg ggccggggca ggtttgctgc cctgacgcga cagccgcatg atcagcaaga
     6121 ccagggcggg cgctgaagtg agcgctacgg gaacccagac ccagggctgg ctcgcgtcag
     6181 taccggcgcc gaaagtgtga gcaagaccga tcagccagaa gatcgcgatg aagcggtggg
     6241 ccgtgcgcca gcgcgagggt ccgatccggc ggcgaaggta atagctgagc cccagcaaga
     6301 tcagcccgta gccggcgatg atcccgattc cggtccagaa cgaccggtac gacatcacga
     6361 agggaaccga gataccggtg atcccgggtt tcagccatgg gtcaccgagc aggagcaggc
     6421 cgtgggcagc gaccagcagg atcgtcgcca tcgagagcgc ctcgtggagc ggcctcagct
     6481 cgaagttctt tctcaggttc ggaaacggca tgccgcggct ggacagcagg ccgacggtca
     6541 ccgaaagtcc ggcgaagagg atcgcggcga tgccggcgcc gcggctgagg acccagaaga
     6601 tgtggatccc gttacccaac ttccggtctc ctctggaaat cccaggcccg gatctcgcct
     6661 tcttcggtca cgaacatccc gccgtcgatc aaccattcgg aagcccggtc gggcccggaa
     6721 agcagcgcag ccttggcacg cacctcggct tccacggcgg tcggggcgac cgcggtgacc
     6781 tgcaccaggc cggttcgagc cggccggccg gtgcgggggt cgatcagatg atgggagtcg
     6841 cccgaaccgg tcttccatga tctgcgggtc gtgccgctgg tggcgaccgc tcccgcggag
     6901 gccgtgaagt cggcgatcac accccggcca gggaaaggtg acttgacgtc gatccgccgc
     6961 tcggcaccac cctcgcctcc gaatcgcagg tcgccggcac attcgaccgc ccaggagtcg
     7021 aagccctcaa gcctctcggc gacgatgtcg gcggccagcc ccttgccgat cccgccggaa
     7081 tcgaggcgca ggcccggcgg gcggatcacg gtcgagtctt cccggtcaac ggtcacatcc
     7141 gaccagctcc aacgccgggc ggggggctcg agctcgggac tcgaaggtcc gggtccggcc
     7201 gggtcgtcgg gattgatcga gtccctgtat cccgcggctt caacctggtc gaggagtgtg
     7261 gcgtcaacca ggccgccgct gtttgatccc gcccggtgga cttcgtctgc gaatcggagg
     7321 aggagtgggc ttgccttcac ggcccggtcg ctgctgcggt tgagctggca gagctcgctg
     7381 gatggttcga agcgggtcag ccggcggtgc agatccagga ccagctcccg ggcctcttcg
     7441 accgcgaggt cggaacccgg gccttccgcc tggatcagga cttcgccgcc gagggcgggg
     7501 aaacagaggg ttgtggccag gtcattcatg actggctggt gaccagcggg ggagcgcttg
     7561 aagtggtcgt ggaaccggtc accgtgcctg ccgtggtcga aggtgaacct tcctcttctt
     7621 ctccgtcatc cccgaatgcc gatgaaacct gcgtgacgag cgcgctcagg ccctcggagt
     7681 catcgctctc gtcgctgcgg gtcacggccg aaacgttggt cgcggccgtt cccgtctgct
     7741 gttccagcga tcggaagagg atggcgccgc tgaacaccgc gaccatcagc gtcgctccgt
     7801 aggcgatccg gcggcggatg ttcccgaccc gaacccggcg ggccagcttc ttcgcctcgg
     7861 ccatttcttt tggggtcagt tcattcatgc tcatgtacct cctcaggact gtccggtctc
     7921 cagcggcgag ggcgaggagc cgccggagga gctttcactg tgttcgtcat cgcccccctc
     7981 tggctgttcg tagctgtcgt cgtcgccata gctctggccg ccgaagtcgt cgtcgccgtg
     8041 gaagggcacc tggccaccag ccggaggtcc gtcgccgtcc ggccggccga aagatgcggg
     8101 tccggcctcg gccgggtcct gcccggtggc ccgcccgatc aacatcccgc tgaagagcgc
     8161 aacgacgacg gcggcaccca ccgcgaccca gcggcccacc gggttgtcgg cgagcctgcg
     8221 ggttgtcgcc gaccacccct tttccggggt cagttccgtg gttgcgggat cgttctgatc
     8281 gggcttctgg tcgtcatgcg tttcgctcat gtccggaagg ttgatccagc cggcttggag
     8341 gatccgtaac gaaacatgag agttctctta gaaacgcgat cccggccgga ttcgcagtta
     8401 cgatcggcac cgatgcgcat cctcctggtc gaagacgaaa aggcgatagc cgacttcatc
     8461 gcccgtggcc ttgaagcgga gggtcatgcg gtgacctggg tcgcggacgg gatcgacggg
     8521 gaagccctcg ccagaaccgg acgcttcgac ctgatcctgc tcgacctgat gttgcccggc
     8581 aaggacggcc tcgaggtgct cgcttcgctg cgccgggagg acgggtccac gccggtgatc
     8641 ctgctcaccg cgaaggggga ggtcgccgac cgggtggccg gactcgacgc cggcgcgaac
     8701 gactacgtga cgaagccatt cgccttcgag gaactggtcg cgaggatcag ggcccagctg
     8761 agaagcccgg cccagggcag cgcgaccgag ctcaggaccg gtgacatcga actgaatctg
     8821 ctgcgccggc gggtcaagcg cggcgatgtc gaggtgttgc tctcggcccg tgaattcgac
     8881 ctgctcgcct acatgatgcg gcatcccggg caggtgcttt cccgggagca gttgctgaac
     8941 ggagtctggg gctatgacta cgacccgggg accaacatcg tcggggttta catcgcatac
     9001 ctgcggcgca agctggcgat ggaaggccgg ccggacccga tcgaaaccct tcgatcggtc
     9061 ggctaccggc tgcgtgacgg ccggtgaaca tgccccatcc cggactgaga ggcaagctcg
     9121 cgatctcgat cgccctggtc ctgctgattg ccctgggggt cacctacttc gcggtctatc
     9181 gaggcaccgg gtccgagctg aaggaccgca ccgagtcgga gctggacaag ggagtcagcc
     9241 agatcgccgc gaggctgaag gtcggcggcg cggacacacc ggcagaacac accgccaggg
     9301 cccatcagat cacctcggcg gagggcttcg gcccgaacgc ccggctggtt tcgatctcga
     9361 tcgaaggcgg tggcactgcc agcaaccagc cggagctggt cgggccgggt ctcgaagatc
     9421 accattccga cggcgagcag aaacacgccg gccacgaaag cggtgacgac agcgaagacg
     9481 agcccgccga gaaaaagcag gatgcggacc ggatcctggg gtcggctccg ggctactcga
     9541 cgatcgaggt tgaagacgcc ggcaaggtcc ggatcctgac ccgggaggtc gaactccccg
     9601 ggggcgtcaa ggcgacgatc cgggtcgggc aaccgctggc tccggccgag aacgcgctcg
     9661 aaggcctcag cgataccttc gtcatcgtcg gcatcgcgac cctgctgctc ggcgcgctgg
     9721 ccggctggct tctggccagt accgccaccc gcccgatgcg gcggatggcc caggtcgccg
     9781 aaggagtcga cgcgggagat ctctccgcga ggatgccggt cgaagggacc cgggacgaag
     9841 cccgccggct ggccgaatca ttcaacggga tgctcgaccg tctcgaggat gccttcgacc
     9901 gtcaacgggc tttcgtcgcc gacgcttcgc atgacctgcg gacccccctg accgtggtgc
     9961 ggggccaggc ggaggtgctt gcccggaacc cgaacccctc ccgggaagag gtcacccggg
    10021 tttacgagct cgtttcccag gcgaccgcac ggatggagtt gatggtcgat gacctgctgc
    10081 tgctggccag ggccgaatcc tccgaaggcc tccggctcga gccgctggaa ctggagccgc
    10141 tgatttcggc cgaggtggaa ggcatggcct ttgaaaacaa tcgcgacctg gtggccggag
    10201 tcgtcaccgg gcgccgggtg aacatcgacc gcgaacaggt cgcccggctg ctctcgaacc
    10261 tgatctcgaa cgcaatcgcc cacacggaag aagggggcag gatcgaggtg tcggccaccg
    10321 accatggatc gaaggtggtt ctggccgtgg acgacgacgg acccggcgtg cccgaaagtg
    10381 accgggagcg ggtcttcgac cgttttgcgc ggctggatga gtcgcgcagc agttcgctgg
    10441 gcggttccgg gctcggtctg gccatcgtcc gggcgatcgc cgaggcccat ggaggtgaag
    10501 cgaggtgtgt tgactcgcca ctcgggggtg cccgcttcga gatcgagttt cccgccgcgt
    10561 gagccggggg cttcggcctt cagccggtag tttgccgcca tgcgcttctc cgctgatcag
    10621 cccctgaacg tccagccggc cacagtgcgt gattaccggg aactcgcccg ccggcgtttg
    10681 ccgcggcagc tcttcgatta catcgacggt ggcgcctatg aagaggtgac tgcacgggcg
    10741 aacgaaaccg acctcgcaaa ggttcttctc cgtcagcggg tgatgcggga cgtatcggtc
    10801 cgggagcagg cgaccgaggt cctcggccag aagctttcga tgccggtgat tctcgggccg
    10861 gtcgggctgg ccgggatgtt cgcgacgagg gccgaggtcc aggcagcccg cgcggcggaa
    10921 gcggccggag ttccgttcac cgagtcgacc gtttcgatct gctcgatcga agaggtggcc
    10981 gaagcgacct ccgtgccgcc ctggttccag ctttatgtga tgcgggaccg cggctacgcc
    11041 gagaagctga tggcgagggc cgaggcggtc ggcgctccgg ttctggccct gaccattgac
    11101 ctggccgtgg tcggcgcccg ccaccgcgac acccgcaacg ggatgggtga ctcgccgacc
    11161 cgggtcgccc agctgaggcg cggactcgac ctcgtttccc atccgggctg ggtcaggcgc
    11221 gtgccgatcg ccggcaagcc gctgaccttc ggcaacctgg ccgaggtcgt gcctggcgcc
    11281 cgcaccccgg aggacttcaa ggaatgggtc gacgcccagt tcgatccttc ggtcacctgg
    11341 gatgacatcg cctgggtgcg ggagaactgg aagggcaagc tggtggtcaa gggcgtgctc
    11401 gacccggagg atgcccgcaa ggcggtcgac gccggggttg acggggtggc cgtctccaat
    11461 cacggtgggc ggcagcttga cgcggtgcca tcgaccgcag ccgccctgcc cggggttgcc
    11521 gaagcggtcg gggacgagac cacggtgctt gccgacggcg gcataagaaa cggcctcgac
    11581 atggtcaaga tgctcgccct cggggccgaa gccgtgatca ttggccgggc ctgggcctgg
    11641 gcggttgccg cggaaggcca ggccggggtg gagaagatgc tccgggtgct gaagtccgac
    11701 gccgacgtcg ccctcgcgct gaccggtcag aactcggtcg ccggcctggg tccggactcg
    11761 ctggtctgag cggggttcag ttcccggtga tgaaccgggc ggccggcatg accaccggga
    11821 gggggacttc cccgccggac cgcctgatcg agaaggtccg ggtggcgctc gtgaaaccga
    11881 tccggaccga aaagacgccg ctgcggttga agctgacctg gcgcgaagtg gccggaccgc
    11941 cgcagagcgg gcgggcccgc agtgtaaacc ggccctgctt cctgaccccg gccagttttc
    12001 ccttgatcag gtcggcatcc ggaagcggct tgcgggaaag gaccctgacc gctccgcttg
    12061 gcttcatcgg attcgacctg cttccaccga cgaccagccg gtagcgcgtc ccggggcgaa
    12121 gccgcttcga tttcggggcg ctgaccgtcg cttcgatcct gccgtctccg gtgacggtcg
    12181 cggtgccggc ccgcttgccg gcccgcagga tcctggcctg gcggccggcg ctggaggggt
    12241 cggcggcacc gagcagcttc acctttgctc cccggggctg gaccgaggcc agcctgaccc
    12301 cgtcagcgaa gcagcccggc ccgacctcgt aggtgaccgt gtcagtcgcg gtcagcccca
    12361 gctcgttggt cgcggtcgcg gtcagggtgt gggtgcccgg ctgatcggtc ggcagccggt
    12421 cgccggtgac cagattcgtc tccccgtccg ggccctggtc cctggcgagg caggactcga
    12481 gatccggccc ggccccggta ccgcaggtaa gcccaaggaa atgagccgag ttgaagtcga
    12541 acgtgagccc gtccccgggg cccgtgatcg acacggtcgg aaggctgccc tgaatcgtgg
    12601 cgatcgccgc cggaatgtcc ggcagcggtc cgatctgccc cagttccgga ctcgcctggg
    12661 gagtgccggt gctgcgaagc aacccgacca gctggtccgc gtccagcggc ggctgaccgg
    12721 tcgccttcag gtaggaaaga accgcggcga ccgcgcccgc gacaaccggc ccggcccccg
    12781 aggttccgtc gaagctgctg gtgtagcgga tgtccttttc gcccggaccc gggccctgga
    12841 ggtcaccgtt actgccggtc gtgaccaggt tgttgccgta gccctggagg tcgatccgcg
    12901 atccgtgcga ggtgaaccag agcggggcgt gagtgaccga gtccccggct ccgaccacga
    12961 tcgcttcgga gtcccggttc gcacggttga aacggccgag catggcgggg gagtcgaggt
    13021 cgtagccccc gttgcccccg gtttccacga ccacgattcc gaggtcgccg agctgccgga
    13081 tcgcgtcgaa gctcgcctga ttccattcca tcggcacgta atcggaaggg ccgggaccgg
    13141 gaccgtcggc ctgctgctcg atcagcacga cgtcaccggg actcagcttg tcggcaaggt
    13201 aggtcagagc cccggccggg ttgtagccga agtcgggtgc catggtcggg gagatgccct
    13261 tcatcgtcgc ctcgggcacg atcccggtca cgccgaaccc gttgtcgtcg gcgttgagga
    13321 tcccgaagac cgcggtgccg tgctcgtcat cgaaggcggt gtactgaacg tactcgccgc
    13381 caccgatgtc ggaggacgca ggcagctgga gatcctcgtg agtccgggtc cagtagtact
    13441 cgaggtcgac cacggtcacg cccccgcccc ggactcgtgg ctcggtctct tcgtactcgg
    13501 cgccgatacc ggtgggagcc atgtcgaggt gaagctggcc cggactgaaa tcgggcgtga
    13561 ccggtggcgg aagggcgacc ggggcccggc cggcataggc gacctggccc gagttgcgga
    13621 gcgcatcgag ggcgtctgct tcagtgcccg ccggtacctc gacccggtac cagtcggcca
    13681 tgttggtggt catggctccg gagagcctgc tggcccgcct cgccaacgcg gtcgactcgg
    13741 cctggctgac cccggccgcg agaggttcgg cgctgatcgt cccgatccgc tcgaagagct
    13801 tctcgacccg gtcaagggcg ggctcgctcg ccgctcgaag actggcttcg tcgcctctca
    13861 gactggcact ccggctgaac cgcacttcga gaaccgaagt tccagggtcg tcgccggcgg
    13921 aggaaaccgc cggtgcggca aagacgggca ggaccgcaaa agcggcgctg acgagaatcc
    13981 ccctgaacct gatgcctggc atcagaccca tgctcccaca gtggagccgc cttgccaacc
    14041 aactagggac cggtcggtgt cgcctgggcc gggcggttcc ggccttccgc tacaagttgc
    14101 gttgctggaa acagggaagg atccccgacc tccagttcga ggccggggat ccgggcggcg
    14161 gcgggcggtg gccgggcgcc gggctgggat gccggcgtcc tggcctgtca actggtttct
    14221 ccttccccct ggatcaggag gtcgcggtcc gggcgctgcc gccggagccg agcgccgctg
    14281 cggcgaggcc gacgaccccg atttcgagca gcaccgagac gactcccgag agttcccact
    14341 ctgcttcgtg gaacccgggc aggccgaccg tccgggagag gatgaagccg gcggccattc
    14401 cggcgctgac gagcgcggca ccgacccagg cgaccggctc gctgcgaacc cagagccatg
    14461 ccgccagcgc gatcgtggtg attccgccgc cgatgaagag gaggccgatg taggcctgct
    14521 cggccatgta ttcgggggcg agcacgagat ggatgacacc ggtgagggcc agggccaccg
    14581 cggccgcgcg tctcaaggtc tgatctttca tgtgaatcct tccgtcagat tgactaggga
    14641 ttcagacctt gatctgccgc gatcagatca tcttcaacgc cgcgttagaa gcttcttatc
    14701 gaccgctcat ttggcgatgc tgacgaccag tttgcccttg ttttcgccct tgaagagcat
    14761 gttcagggcg tcgggcaggt tctcgaaacc ttcgaccacc gtctgctcgg atttcagttt
    14821 gccctcggca acccagcccg aaagttcgcg gatcgcctcg ccgaagcggg cgtagtagtc
    14881 gaggatgatg aagccctgca gcctgacgcg gttgaccagc agccggccga agattctcgg
    14941 gtcgagcggg cgcaggtcgt cgttgtaacc cgagatcagc ccgcagaggg ccacgcgggc
    15001 attgatgttg agacgcagca ggaccgcttc catgatctcg ccgccgacgt tctcgaagtt
    15061 gacatcgatt ccgtccgggg tggccgcccg gagctgctct ttccagtcgt cggccttgta
    15121 gtcaacggcg gcgtcgaagc cgagctcgtc gatgagccag gcgcatttct ccggaccgcc
    15181 ggcgatcccg accacccggg cgcccttcag cctggcgagc tggccggcca cggtgccgac
    15241 cgctcctgcc gcggcggaaa cgacgaccgt ctcgccttcc ttcggctcgc cgatctcgag
    15301 catcccgaag taggcggtgc agccggtcat gccgagcatg ccgagcaggg cggcgggagg
    15361 agcggacctg gcgtcttccg gcaccaccat gagcggattg gagtcactga tcacggtgta
    15421 atcggtccag ccggtcaggc cggtgaccag ggcgccgacc gggtaattcg gattgttcga
    15481 ttcgaccact tcgccgaggc ccagggcccg catcgtctcg ccgatctgga ccgggggcag
    15541 gtaggtcggt tcttcaccga tccaggtccg gttggtcggg tcgatcgaga tccaggtgac
    15601 ccggaccagg gcctcgccct cgccgatctc cgggaccggc cccgtctcca gggtgaaggt
    15661 gtcctcgtca atcaggcctt cgggccgctc cgccaatgca taacgccgat tctccgtgct
    15721 catatagaag aacctatagt ggagcaagga gagagggaga agctgtggga aaaacgcgta
    15781 gagacctgct ggttgatggg accaaagtgg cggccggact ggcggcggcc aacgcgcttg
    15841 cgccgaccgg acccttcggg gcagcgcagg cgttggctga tccgaagaga aggctgcgcc
    15901 ggcccggttt cggcctcgca ttcaagtcgc tgaaggacga gaccttcctg ccctccatcg
    15961 atcttgacgg caagttgccg cagtggttga ccggcagtct gctgcgcaac ggcccggccc
    16021 tcttcgagat cggcgaggaa aagttcaacc actggttcga cggcctcgcg atgctccacg
    16081 ccttctcctt caagggcggc aaggtcagct accgcaaccg cttcctgcgc tccagccagt
    16141 actgggcctg gcagcgtgaa ggccggatca agttctccga gctggccacc gacccggccc
    16201 cggacccctg ccgccagatc ttctccgggg tctcgaccct gccggtgctg ggccggatcc
    16261 ccaacgcaaa cgtctcgatc gagaaactgg cgggcgagtt ccgggcccac accgaaatcc
    16321 cgatcccggt ccgtttcaac ccgaaattcc tgaacacgat ggggatcggt gatgccgggc
    16381 agatgcaggg ccgcctcgga acggcccatc cccacttcga cccggaaacc cgggaacgat
    16441 tctcctacga ggttgaactg gtgccgccga gcgggctccg gatcgtctcc gaaaaaggca
    16501 ggacccggcg ggagctcgcc ttcatcccgc aggcgatgcc gggttacctt cactccttcg
    16561 gtttgacccg gcggttcgtg gtgattgtct cccagccctt caacttcaac ctctcgaaat
    16621 tcctcagccc ggaccgcggc ccgatcatca ccaactacga atggcagggc gaggagccga
    16681 cccggatcat cctggtcgac cgccagaggg gtggtgtggc ccacacggtc gagaccgact
    16741 ccttcttcgc cttccatcac atcaacgcct acgagcatga gggcaaggtg atcgtggacg
    16801 tctgcgccca caaggacgcc ggggtgatcg acgcgctcta cctgaagaag atccgttccg
    16861 gcagcaaggt gccgcaggcc aggtatcgcc ggttcgagat cagacttgaa ggtcagggga
    16921 agctgagcca gcgcgacctg atcgacgcct acatcgagct gccgcagaag aactacggcc
    16981 gggtcaacgg caggcattat cgctacgcct acggggtagg tctgcgcaag ggatcgagcg
    17041 gcttcatcga ccagctggtc aaggccgacg tgaagatagg cgagtcaaag ctctggcgcg
    17101 aatggggggt atacccgggc gaaccggtat tcgtgccgag gcccggaggg aaggcggaag
    17161 acgacggggt gatcctctcg gtcatcctcg acggccggcg gcgaaagtcg gctttgttgg
    17221 tcctcgacgc gcggaacatg gaggaaattg cgcgtgcttc agtgccgcat cacatcccct
    17281 ttggcttcca cgggctgtat gcttcgtgaa caggggtgtt tacatttgta gttgacgggt
    17341 acttccgtac tcaggccttt ggaggactga tggagcgcgt cccgccacaa cagaacacag
    17401 gcagtctccc gctggtgatc gggcttaccg cgctggtctt ggtcgtcccc tgcgccgcgg
    17461 cgattgtgct cagggccacc ggaatcgtca cctcgccggt gttgctgatc ctgatcccgg
    17521 tgctggtttc cgttctcttt tcccattcga tttccgagtt ctggaagcgg cgccgggccg
    17581 gcagtcccct gctcttcgaa gacctgatgc tgtggggctg gctccgccgc cgccgcttcg
    17641 aaaggctgct ttcccgctcg gaggacttcg tcggcccgga ggcgggccgc gggctgactc
    17701 cgaagcagcg ggcccgtgag cttgaacgcc tggccagcgc cctcgaggct cgtgatccac
    17761 gcacccacgg ccactcgcgc cgggtggcga ggcatgccac ctcgatcgcc cggggcctca
    17821 ggttgcccga tgccgaagtc gcccggattc gcaccgcggc gctgcttcat gacgtcggca
    17881 agatcgaggt gccctgggaa atcctcgaaa agcccggcgc cctgaccgac gaggaattcg
    17941 cccagatcaa gaagcattcc gccgccgggg cccgcctggt cgaagggatg ggtgacccgg
    18001 agctcgtcgc gatcgtccgg caccaccacg agcggattga cggcggcggg tacccggacc
    18061 gcatcgccgg tgacgaaatc ccgatcgggg cccggatcat cgccgttgcc gacaccttcg
    18121 atgccctcac ctcggcccgt tcctaccgcc agcctcgttc gcatgaggag gcgctcgggg
    18181 tgctccggga agaggccggg gcgcagcttg atgccggcgc ggtccaggct ttcgacggcc
    18241 gctattccag ccgccggccg gtcgccttgg ttgccacggt cctcgggctc ggcaggcagg
    18301 ccggccagtc gatgatcagc ttcggaaccg gtgcttcgca ggtcgcggcg gtcggggcgg
    18361 ccgcggcagt gatcggtgtc gcccccgcgg ccagggtgga ccagcagcag gaaccggcga
    18421 agcagaaccc gatcgtgcgg cagtcggatt ccacctcggc ccggtcggtg gctgattccg
    18481 gctccgcaac ggccggcagc cagtccggga ccggagagca gggccgcaag tcggcatccg
    18541 gcggccggaa cgcccgaaac ggcaacggcg gcaaaggatc aaccgataac accttcagca
    18601 cttccgggga cggcggcgga accagctccg gatcaaacgg tggctccggg tccggtggag
    18661 gatcctctgg tggctccggc ggcggcagcg gcggcggttc ttccagcggc ggctcgtcgg
    18721 gcagcggcac gaccgcggtc aaccctgtcc agacggtcac cgagaacgtg cagaagcagg
    18781 ttcagcagac cacccagcag gtgaccgatt cgatcccctc cctgcccggc aacgacccgg
    18841 tttcccaggg tgtcaacaac acggttgacg aggtcaagga cacggtcaac agcacggtcg
    18901 gcaagctcac cggcaagccc tgaccatttc ccctagcgcc aggcgtggcc gacgaagacc
    18961 ggctccggga ccagcgccac cccgaagatt tcttccaccc ggtcggcgat gccgtgggcg
    19021 aaggcgacca gttccgcggt cgtgcccccg ccgcgattga cgatcgcaag ggtgtggttc
    19081 cttgacagcc cgatcggtcc atccccgaaa ccgcgggtga atccggaccg ctcgataagc
    19141 caggcggccg aagtcttggc gaggccttcc ggcgccgggt agcccggcgg caggtcatcg
    19201 gctccgaaca gttcctccgc ccgggcggtc aggtccgcca tgctggccag atcgagcagg
    19261 gggttggtga aaaacgagcc ggcgctgacc gaatccggat cggccgggtc gaccaccatg
    19321 ctcttcgagc gcctcagctc gagcacggtt tcgcgggcga ccggcagggg aacccgttcg
    19381 ccgacctcca cttcgagcgc cccggcgagc tgggcgtact cgaccggcgc cgacatctcg
    19441 gaagggctga ggatgaactc gaccgccagc acgagaaagc gcgaatcacc cttgaagcgg
    19501 ctcgaacggt aaccgaagcc gcattcgctt ccggcgaggg tgacgacttc ggcgttctcg
    19561 cgatcgagca ccaggacgga agcgatggtc gatgagactt cctgaccgta ggccccgacg
    19621 ttctggatcg gggtggcgcc gaccgttccg gggattcccg agaggcactc gattcccgag
    19681 agccccgaat cgaccgtccg ggctaccagg gcatcccagt tctcgccggc agcgacggtg
    19741 agagagaccc ggccgtcatc gagttcacgc tcgtcgattc ccgcggtccg gatccagacc
    19801 gtggtcccgg gcaccccctg gtcggcgatc accacattcg acccgccggc gaggaagaag
    19861 agcggttccc gctcggcgtc agccgcctgc accagcctga tcagctcggc cgtgtcttcg
    19921 accttgatca gccgctcggc cggcccgccc agcttcaggg tggtcaaggg ggcgagttcg
    19981 acgttttctc cggcttcggc cacgccccga tgcttccaga aaacggggag cggctgtgtt
    20041 gacacaagaa aggggccgca tcagcggccc ctttccatgc gatctgttgg gggtcagatc
    20101 acgcctgggt ggaggtgtcc cccgccggga caacgatctc cagttccttg aagaccttgc
    20161 ccgaggccgt gtacttgacg acggcttcgg tcacttccac gccttcggcg aggtcgtctc
    20221 ccgagcagtc accgccacag tcaccttcgt gaccggggcc ctctccgtcg tggccgcggc
    20281 cttcgccgtt gccgtgatcg tcatggcctt cgccattgct gccggggccg ccgtggcggt
    20341 cttcggattc tccggagccc tgatcgtcgg ttccgtcgtc gtcaccttcg tcggttccgt
    20401 tgtcgtcacc ttcgtcggtt ccgttgtcgt cagcgacgtc ttcggattcg cagtgaacgc
    20461 gggtctcatc ggtgaccgaa ccggtcagct cgccgcccgc gtaaagggtg atcgtcagat
    20521 cgccggtttc cgaatcgtag gccgagatga caccggcctt ctccttgagg tcattgactc
    20581 cgtcgtcgtc cgagtccttg tcgcgcgggt tggtgccagc cagccactcg ccgcgattgc
    20641 gaagcccgtc cgtgtcctga tcgagcttgc gctggtcccg cttcagcgaa agcttgtggc
    20701 tccgctccca gcggtccggg atccggtcct tgttgtgatc ctttgccgag gcggttcctg
    20761 gcatcacgat cacaaacagc gccagaatgg ccgccgcaag gatgccgaac ctttctctcc
    20821 tcctgatctt catgctttcc tttcgtgcga ttgcttggag cttctgaacc ggagacccga
    20881 gccgaaacga aaagtcacgc gaaaagttca gctggatccg ctttttcgcc tgtagaagcg
    20941 aaaaaaggga tgtcaatcgt cgtcagaagg atggggcgtc ctgccgccct caccaccgga
    21001 agaccccgac gagccggatc ccgagtcatc ggaaccatgc gagccacccg aaccgctcga
    21061 accggacgaa ccaccggacg agccaccgga actgcccgaa ctgccacctg agctgccgcc
    21121 ggaactgccc gaactgccgc ccgaaccgcc ggaaccgccg gagccgcccg aaccgccgga
    21181 accgggatgc cagtccgaat catcggattc gtccgacccg ccggaggggc cgtggccgct
    21241 ggagtcatcc tcgcgcggag gaccgccgct cgggccggga ccaccgccac tgaccccgcg
    21301 ggagggccga tcgtcgccgt ggccgccgtt cccggaatcc tcgtctgaat cttccccggg
    21361 ctcatgccgg ccgccggcgg gtcggacgcc caggggatgg tcatcgccac ctgaaccgcc
    21421 cgaacccggg cccgaggaac cggaaccgcc ggggcgggag tcgcctgagt cgcggccgcc
    21481 ttcacctgtc tgacggcccc cttgactgcc gcgatcgctc tcgccccggt cttcggcgcc
    21541 atccgatccc ttgtcggaac ccgtccggct tcggtctgcg accaggctgc cgttaccggg
    21601 accgtcatcg ccaccggaat cctcgccgtt cggagccgcg gcgccggaat cgcccggctc
    21661 ggccgcctgg gcggtgtccg gatgggtacc gcggtctgcc tgatggcgtt caactgcgat
    21721 gccgccggcc agcgacccgc ccgcgaccac ggcaacaacg gccagcttgg cggcgctgcc
    21781 gccaccgatc gcccccatca gcccggtcgc cgtcgctccg gtgcccgcgg cgccggtggc
    21841 caccacgccc gagccttcgg ccagccaggc gatcaacggg atcgggatca tcgccccgaa
    21901 ggcgttgcga agcgaaagcc gggcccggta gatgagttgc ttggttgccc ccgtgctgag
    21961 atcgagttcg gccgcgatct cctcgtgtga gaaaccactc agttcgtgat cgaccaaggc
    22021 ccggcgctgt gcgggaggga ggtcggccac cgcggcgagt acggcggcga accgttcgcg
    22081 ctgttcgacc agctcatccg gggaggttcc cccggtggcc gtctcgccga gcccgtcgtg
    22141 acggtgcttg cggttgtcgc gcacgtcatt cagagcggtg ttccggacga ccgcgtaaag
    22201 ccagggccgt gggtcgatgt cacggtcatc ccgaagggaa cggtgcgcct tgaccagagc
    22261 gtcctggacc acgtcctcgc cccggtccgg cccggcgatt gatccggcat aggcaaccag
    22321 ggtcacccgg taccggttga tcagttcctc gaaggccggt tcgactccgc gacgaaacag
    22381 cttcagcagc cgctcatccg actggagttt cagcgtgccc cgggccagtg gccggatcga
    22441 tcctggattt tctgccgcca tctcttcccc tcaggttacg ccgtgaccat aagagccacg
    22501 tgagaatctg aacctgcaac ccgaaccgga cacggaagtc acgcatccgc cgtcccggca
    22561 cggttgggta atcgcaggcc acaccgctgc ctgcctgggg ttgcgggcgg cagtatgctg
    22621 gccccgtgag cagacgggta ctgattctgg gagcgggctt tgg
//
LOCUS       DAOQKN010000015        21435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_1061_length_21435_cov_6.305192, whole genome shotgun sequence.
ACCESSION   DAOQKN010000015 DAOQKN010000000
VERSION     DAOQKN010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 21435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21435
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1061_length_21435_cov_6.305192"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..1823
                     /locus_tag="PLJ64_02055"
     CDS             24..1823
                     /locus_tag="PLJ64_02055"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HNC92309.1"
                     /translation="MRLSEGMVVDGRYRIDYLIGSGGMADVWLAEDLELPRRVALKVL
                     HERYARDPEFIARFRREAESAASLQHVNIVSIFDRGQVGDTYYIAMAYLEGRTLRDLI
                     SLGLTPPESVAIVRQILEAAGFAHRRGIVHRDFKPLNVIVDETGLATVTDFGIARAGI
                     SEITEEGSIMGTVHYLSPEQAQGYEVSPQSDLYSIGVILYECLTGRVPFDGQTPVAVT
                     LQQLKEDPVPPSAYNRAVSPALDAVVLRALQREPYERYPDAESFIAALDAAEADPGVP
                     EEKSNWWKWALVVLAVLIGLLIAWAATRSHTVEVPDVVGTSSDAAVSKLNQEGFKVEV
                     DREKNPSVPNNQVYRQDPSGEADQDCNFLGFSCSNPTVTLTVSGGPGQTEVPDVTGLS
                     QDDAEQELEDAGFTTSIETRTSSDVDSGLVIETDPAGGEMARRGSEVTVTVSSGAAQV
                     KVPPVVGQTLNAAKQQLAAVGLDYSSSEEPSDRPQGEVIDQSPTAGTRVDPGSTVTLV
                     VSSGPEDTRVSVPALVGQTQSSAESQLTAAGLVPSVQTQSTTIQPQDGKVIDQSPGSG
                     TKVAEGSTVVITVGKYEPDSGTTGSDTGGIGID"
     gene            1872..2876
                     /locus_tag="PLJ64_02060"
     CDS             1872..2876
                     /locus_tag="PLJ64_02060"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HNC92310.1"
                     /translation="MRVAVLSGGRSSEHEISLLSGEAVVEGLEAAGHEVIRIQIEKSG
                     LWVSGGQAVEIRPGEGLTVDGDLLSLDVVFPVLHGPFGEDGVTQGALDTVGVPYAGSD
                     VLASAVCMDKLTLKRLCGFHGIPQVGFVQAFTPGWEEEALGLEFPLWVKPSRLGSSVG
                     ISKVTDPAGLTTAVEEAARFDPRVIIEANSAGREIECSVLGNENPEASLPGEILTDDE
                     WYDYEAKYTEGRTRIAAPADLDEPTTAAVRDLATRVFTMAGCSGLARCDFFVEEPAAG
                     ESGQARILLNEINTIPGFTRTSVYAKLWEATGLPYPDLCNRLAELALDRHKSESDYTF
                     "
     gene            3675..>4189
                     /locus_tag="PLJ64_02065"
     CDS             3675..>4189
                     /locus_tag="PLJ64_02065"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HNC92311.1"
                     /translation="MERALRDFAAEATPAEMTKALTQGEKERAICWGTLRELVADSAG
                     HKGNTMLSTEIEGWNPAFVDTGSDPEIDFLRMIRKREMPWPAVNVKLGHNVPDFLWWH
                     LRLAVELDPYGTHSGAGSHRRDHRKGIELETMGLRVVRFTGEDLYRHEERTGDELWIV
                     IRQQSRLLRCP"
     assembly_gap    4190..4289
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4290..5796)
                     /locus_tag="PLJ64_02070"
     CDS             complement(<4290..5796)
                     /locus_tag="PLJ64_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNC92312.1"
                     /translation="MNHPPASSQMIGTVLSDRYRLEAKLGSGGMSTVYLARDEVLDRP
                     VAVKLMHREMTEQPDQLERFNQEARAVAKLSNPNVVAVIDAGEDQGRPYIVLEYVQGE
                     TLKQRIARVGALDATEALAYGLEVAQGLGVAHERNMVHRDVKPQNVLIDSTGRAKLTD
                     FGIARELNDEGVTATGRVIGTTDYVAPEQAMGKEVDPRSDIYSLGIVLYEMLTGDVPF
                     EADNQIGVAMKHVNEELPDVQVIRPDISAASARVVDRATAKNPDDRYQTIEEMIDDLQ
                     AALEVEAIRAGGTTGEATSVLDAVAPPRRQIPTSKTSPWPAVLMLLVALVIAAGTAYF
                     ISRGDTGGGGGGGAGGGGSNETQIALVGASDYDPQGDEEEHSEEIDYAIDDDRSASAW
                     TTETYETDSFGDKEGVGMYVDAGEPVEASEMEIRTPTPGWTLEVYGSNDPIPDDVSGW
                     TRLAQVPDVAEQQQIDLITAGTRYQYYLLWITDPAETDSGFGVAISDIRLYG"
     gene            complement(6046..7056)
                     /gene="purM"
                     /locus_tag="PLJ64_02075"
     CDS             complement(6046..7056)
                     /gene="purM"
                     /locus_tag="PLJ64_02075"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HNC92313.1"
                     /translation="MSGEDAYAKAGVSQSDADLAVSRLVAALATANIGRPTRQVDLKG
                     HYAAVMRLDNRTGIALSTDGVGTKLLVAEELGRFDTVGIDCIAMNVNDIICVGAEPVA
                     MLDYLAVEKADPEMCEQIGVGLARGAELAGIEIPGGELAQIGDLVKGFDIAGACFGTV
                     ALDSIIDGSAVAPTNVVIGLPSSGIHSNGYTLARSALDGIGMDDDRLGRPLGEILLEP
                     TEIYVKAVLELLASPVRVHGLAHITSGGLDNLLRLKAEVTYEITDPLAPQPVFELIAE
                     RAGVPDEEMYEVFNMGCGFCVVVPAEDESAALEVLRGHYPEARRIGDVAAGDRRVVRR
                     TR"
     gene            complement(7084..7875)
                     /locus_tag="PLJ64_02080"
     CDS             complement(7084..7875)
                     /locus_tag="PLJ64_02080"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNC92314.1"
                     /translation="MVLGLVQGPAELLPISSSAHLAMLPGLLRWDPDELDPELRKSFE
                     VALHGGAALALLITRRGEIRDEIGEFDLRRASVLLLSFLPAAAVGYGFERVIESRFGG
                     PRGTATGLILGGLAMALADRRPQERGRGEADWKDGLALGIGQAAALIPGVSRNGATLT
                     AARLRRFSRDQANLLSRTVALPVILGAVGLKGVRLARRGISRRQARWLAIGTATSFAS
                     TMAATDLIRMVERDRALAPYAAYRIAFALLILARLAADRGDRAAE"
     gene            7960..9843
                     /locus_tag="PLJ64_02085"
     CDS             7960..9843
                     /locus_tag="PLJ64_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HNC92315.1"
                     /translation="MRRPDFHLRRYQLMQMAGDGLLVALSYFLAFQLRFLDDPGTIPE
                     RYVDLFLQSVGFVIVGKLIVFYLFGLYQKWWRFVGGRDIARIVQAVSVSSLLLVVLFT
                     VIQPFPHDLPRSVAVTDFLLTLFLIAGARLATRLFKERPNKGNRIARGREVLVVGAGS
                     GGQMVVRELRINPNLGETAIGFVDDNTEMRGMRIQGIRVLGTTDEIETILDETEPDEV
                     MIAIPSAPGVLRARVVAACRERQIRVRTLPTVFELLRGGVQLSQQLREVQVEDVLGRD
                     PVTMELARVGAYLEDQVVMVTGAGGSIGKELCRQIGLVGPRLLVMLDHAEDNLFQIER
                     EMVAERHFTRVEAVLADCKETERIFEVMQRFKPNIVFHAAAYKHVPLMESNPLEAVRN
                     NSFATRITADCAAAAGAERFVLISTDKAVNPQTVMGASKAMAEWAVAAADAHHANTRF
                     VSVRFGNVLGSSGSVVPIFKRQIEQGGPVTVTHPEMTRFFMTIPEAVQLVIRAGDTGV
                     SRGDVFVLDMGEPVKITDLAENMIKLAGREPEKDIAIEFTGPRPGEKLHEELFGVEER
                     PQPTASKRIMRAVREEPIDPEWIVQTLDRLDQVVQTGDEANLAETVVELASNPGSDPA
                     KVI"
     gene            9964..10692
                     /locus_tag="PLJ64_02090"
     CDS             9964..10692
                     /locus_tag="PLJ64_02090"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HNC92316.1"
                     /translation="MTDVIQTVGSYAGLVALLAIGVLAFLVFSQARDIRRLREWAGGA
                     PERDAEVREVSEIVAEERSAELRVLADREERRMERAGLAEPSFWDRLGRSGRIIAIVA
                     AVLILGAAAAWAATSLLGSDSGNSGNGKGGNAAKSQNAKPAQIRVAVLNGTGGTESGL
                     ANTYATALEDDGYQIGVVTDATDTFTESVVMFASGHQAEARKVGKAVGIDNTAPMDAA
                     ISGLSSGADVTVVIGTDHGPLPGE"
     gene            10693..11376
                     /locus_tag="PLJ64_02095"
     CDS             10693..11376
                     /locus_tag="PLJ64_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92317.1"
                     /translation="MDNPAGPRGPGTTRLGWLAPLTIGLLLVATVAAFGWSQRLKREP
                     LVIDRVEYRVLGYTPNGESPTVFSPNGDCRRDRMSIHFRTTRSDTADVEIIGLGGRTV
                     RTLARDRFFKRYREHTLIWNGRKDDGGIAFTSKYRVRVTMHGEDRVLYLPGFIRLHRY
                     APRGPSGCPKPLKWKYSVKEPGKPARPLTRAEVAKMTRPLTEAEVAKIKAAAKKALGT
                     GGTTGGQGQ"
     gene            11373..12890
                     /locus_tag="PLJ64_02100"
     CDS             11373..12890
                     /locus_tag="PLJ64_02100"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNC92318.1"
                     /translation="MIEALAALVAAAALAVAILDQPSMRRSIAMAVAGIMVPVLIAGD
                     QWHSGPITELRNSPAMLVLALLCALAVVALIAWVVDRWPVMLPVLVLVALPFRIPLNV
                     GGEDANLLVPLYLVMGGGVLASLIRARRNDLLPALPTVAPAGLARWLKPLLAAAVVLY
                     ALGLFYTDDRSTGLQNLCFFLIPFGVIFALMAEVEWTRDRLRILLGVVVGLALVCALV
                     GFVEYATRDLLWNQVVIRSNDFHVYFRVNSLFWDPNVYGRYLALAITMVAAALLWAKP
                     SRESVILAVVAAVLWLALMTTYSQSSFIALIAGLATLIALRWSLKWVLAGLVALAVGV
                     ILFGTFAGGLVKLDLGAINKQTSGRANLVEGGMDLFEARPLYGYGPGSFSVAFKREVA
                     GPNAPVTESHTEPVTVAAEQGLIGLAFYLALLASILAALLAGMRKVMPGLGAPPRSYD
                     PDRGPPAARAAILAAFVAVLVHTLTYAGFLDDPVTWVLIAIGYSLAFPCRATSAA"
     gene            12866..14449
                     /locus_tag="PLJ64_02105"
     CDS             12866..14449
                     /locus_tag="PLJ64_02105"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HNC92319.1"
                     /translation="MSGYLRRLATTGAAYTAASIISKLIAVALLPLYTRYLTPADYGA
                     AEVMFSAVVAASIIIRLGVVEALLRFYYKQGEEPAAVVSTSFATLFWAGLISVIVLMP
                     FAGPISQLLLGESEPTLGRIAIVGLFVLTLSEYLLTIFRLDERARAFFTVTMLSVLIT
                     IPVTVILVVPLDLGAEGLLAGSYGTGLAIVLGLIFYNRRRLALAPDRPLLKRMMAFGL
                     PTMPAELSLYSLSFIDRILIARTLGLAEAGLYSIAIKMSQGIAVLVRGFQLAFPPLAY
                     SIQDDDEARLAYSAVVTWFVTVMAFVVSFLWLIAPWIVGLLAAPEFSEAAVVVGPVSA
                     GAALYALYMVLLVVLGRTGRTEYNLPATIAGLVVNVGLNLLLLPLWGIIGAGVALVVS
                     YLVVVVLMYLFTQRLFPVPYEWLRLVRVLLATAALVAIGEVFVPDDGLAWLAVRLLLV
                     LALPFALLLTGFFTAEERGWLLLLTKPGELKAKILAAREVGKAAGEEGEGTSASGRPG
                     SRGLPEAVEVEQWDEDLRS"
     gene            complement(14507..15472)
                     /locus_tag="PLJ64_02110"
     CDS             complement(14507..15472)
                     /locus_tag="PLJ64_02110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC92320.1"
                     /translation="MNSPSPAPDPATEATEVDLSYCVVNNDGREYLMDCLAAIEKFHP
                     AGLESEVLVLDNASSDGSVEAVREQFPSAEVIAMESKQGKAANDSLLLERARGRYCLL
                     LNEDSELRAGAAGALAAALDAEPEAGAAGAQLFDGEGNAYACAWRFPGVESAFVGALF
                     LHSRLTVESKGEETRRVDWAQSSALMVRREAAREIGGLDPQFFVYYDECDFCKRLADA
                     GWSTLYVPSAEAVHHNQLSTDLSSGLPRIVEFHRNRDLYMRKHHNRAAAFTVRLLTAW
                     AYGLRTIASVFMPGAPTPVYRAHTRQALFPYRGESLADRAGSPES"
     gene            15475..16656
                     /locus_tag="PLJ64_02115"
     CDS             15475..16656
                     /locus_tag="PLJ64_02115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNC92321.1"
                     /translation="MPTIIPVVHVKLIDPSAFTPPYDRSLAAALAAEGAEVELITSEF
                     TYGPVPEPEGYEVSLDFYRRISPRARRFSKALQHFPEMRRLRRRLENAPDPMVTHYQW
                     LTMPRFDHRLISKRRPRLMTAHYILPPGPNGKQAAAAKRVFGAMDAVIAHSKGGASRL
                     VEEVGLPEERVRVIPHGAFDYLTRLPEEAPLPPELADSGAEAPVILFFGLLRPYKGID
                     VLIRAAGQLAADADRDGPAPELWIVGNPRMDLTELVERSRKLKVKVRWLPRFIEDQEI
                     PAIMRRADVVVLPYLDGEQSGVLYTGIAFGKAMVVSDVGGIGEVAREHGLARTVEPGD
                     VDQLAAELSALVLGDRAAAAREELETRCRVAAEGPFAWAAVARQTIALYEDLLGRGSG
                     S"
     gene            16653..17831
                     /locus_tag="PLJ64_02120"
     CDS             16653..17831
                     /locus_tag="PLJ64_02120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC92322.1"
                     /translation="MIVVYILFWLSAGLVFFTHIGYPITLWLLDFIGLGGRAGEVRGR
                     KLSEIPPAIDPDTEEEILPSVSLIIAAYNEETVIEAKVKNALELEYPRELLQVIVASD
                     GSSDRTVEIAREAGADLVLDLPRGGKIAAQNAAAEAATGNLLAFSDANSTWEPGALAE
                     LIDPFRDERVGYVCGQVRFIGPDGGNLEGAYWRYEMRVREMESELAGVTAGNGAIYAV
                     RASAYIPLAPSGSHDLSFPFALAKRRLASLYRPRARATEKLVPSLDGELARKRRMMVG
                     LWDIVVGEGMLDPRGYSPLYLFELFSHRLLRYLSPALHLIAFLTNLALLGHGWFYGLT
                     MLLQLLLVAGAILGRWIPILPFRISRYYLEVTLSIALGLWDRWRQGPPAAWEKAEGTR
                     "
     gene            17863..18426
                     /locus_tag="PLJ64_02125"
     CDS             17863..18426
                     /locus_tag="PLJ64_02125"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNC92323.1"
                     /translation="MSRGFEKLVTALVLVITAPLLVVCAILIRLEGGGSAIYRQTRVG
                     LHGEEFEMLKLRTMIPGSDPVGVGTVVGREDPRVTRVGRWLRRTSLDELPNLVNVWRG
                     EMALVGPRPTIPAQVADYTPHQHRRHEVRPGITGWAQVQGRAGIPWDERIELDVEYVE
                     NRSLRLDLEILRRTVWLAATGQGLAPD"
     gene            18453..19358
                     /gene="metF"
                     /locus_tag="PLJ64_02130"
     CDS             18453..19358
                     /gene="metF"
                     /locus_tag="PLJ64_02130"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HNC92324.1"
                     /translation="MRIGQIIGAKRPVFSVEFFPPKTDEGRAQLFDTARQLAELELDF
                     VSVTYGAGGSTREGTVDITTALKDEVGLEVMAHLSCVGETADGLDATLERLRDAGIDN
                     IFALRGDPPRGETDFVQPEGGFGSSAELASHINSKYDFSIGGACFPEVHPEAPDLDTD
                     LTYLKTKVDAGAEFLITQLFFDNRAYFDFVDAARAKGIEVPILAGIIPVTGYAHTKRI
                     CNLCDASIPVELEAAMLAAEGDSEAEFNLGVAYAAQQSAELLAAGAPGIHFYALNKAP
                     ATRAVLGALRAAQPWRGARGQSLTS"
     gene            19369..20085
                     /locus_tag="PLJ64_02135"
     CDS             19369..20085
                     /locus_tag="PLJ64_02135"
                     /inference="COORDINATES: protein motif:HMM:NF022747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HNC92325.1"
                     /translation="MGQEGNREVKQPTRAGYAIGAVFLLFIVGGAFFALRSGGDDGGE
                     AHISTASGSSNGLAPDTRKGTPYEGPLITDLARAAAAAKCVVREGLPEEGHDHINRGD
                     PVPEYRTVPPTSGDHIVPPLQQADGAWADPVAPVDVIHSLEHGRVAIQYSPALPEEDQ
                     LALKGLFDSAYSAALLFPNPEMPYAAAATSWRNLIACPRWQGRKTLDAINAFGVAHWG
                     QGREPMAGFPALSGPSFGDP"
     gene            complement(20138..21235)
                     /locus_tag="PLJ64_02140"
     CDS             complement(20138..21235)
                     /locus_tag="PLJ64_02140"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC92326.1"
                     /translation="MRLAAELVADGGRAASGPDFFRSAEFLEAEGTTHSLLIFEDGDE
                     TELVASTPLIVREIPGSDRLDAISPYGYPGLAISDGPLLRLDFPLDPEAIDFTGTGLV
                     TIFLRHSLEAPSGEVPHVSDRDGAERAAPLEERDPLAAGPPLAGATPRNVCLLSDPAL
                     PRKSRASDRQQIRKNLKRGCSLEITPGPETTPGERAGFFTAYTETMARTEAADRYFFS
                     EKYFDRILSAEGTWLFLLRAPEEEADADGRRPVAAASIAALSDGLLHYYLSGTADAHL
                     RGAPMKNVVEEMIGFAAERELALNFGGGITPGDALEEFKRGFANTERQWFTSEIVCDG
                     TAYETLTARLAPNADSDFFPRYRSQSRPNHP"
     gene            complement(21255..>21435)
                     /locus_tag="PLJ64_02145"
     CDS             complement(21255..>21435)
                     /locus_tag="PLJ64_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92327.1"
                     /translation="RIYGRIPAFNEAAKKVVTAQGWTYEGNMPGHIRRPDGTMLDCEI
                     WGATPEQFFAAVGKW"
BASE COUNT         3387 a         7248 c         7108 g         3592 t
ORIGIN      
        1 ggaacccgaa aggcaagagc tagatgagac tgagtgaagg catggtggtg gatgggcgct
       61 accggatcga ttacctgatc ggctccggcg ggatggccga cgtctggctg gccgaggacc
      121 tggagctgcc gcgccgggtc gccctgaagg tgctccacga gcgctacgcc cgggaccccg
      181 agttcatcgc ccgtttccgc cgtgaagccg aatctgccgc ttcgctccag cacgtgaaca
      241 tcgtttcgat cttcgatcgc ggccaggtgg gggacaccta ctacatcgcc atggcctatc
      301 tggagggccg caccctccgc gacctgatca gcctcggcct caccccgccc gagtccgttg
      361 cgatcgtgcg ccagatcctc gaggcggccg gcttcgccca tcgccgcggc atcgtccacc
      421 gggacttcaa gcccctcaac gtgatcgtcg acgagaccgg cctggccacg gtcaccgatt
      481 tcggcatcgc ccgggccggg atctcggaga tcaccgagga aggctcgatc atgggcaccg
      541 tccactacct ctcgccggag caggcgcagg ggtatgaggt gagtccgcag tcggacctct
      601 actcgatcgg ggtcatcctc tacgagtgcc tgaccgggcg ggtccccttc gatggtcaaa
      661 caccggtcgc ggtcaccctc cagcagctga aggaggatcc ggtcccgccc agcgcgtaca
      721 accgggccgt ctcgcccgcc ctcgacgcgg tcgtgcttcg cgccctacag cgcgagccct
      781 acgagcgcta cccggacgcc gaaagtttca tcgccgcgct cgacgcggcc gaagccgacc
      841 cgggtgtgcc cgaggagaag agcaactggt ggaagtgggc gctggtggtc ctcgccgtcc
      901 tgatcggatt gctgatcgcc tgggccgcca cgcgcagcca cacggtggaa gttccggacg
      961 tggtcggcac cagcagcgat gccgcggttt cgaagctgaa ccaggaaggg ttcaaggtgg
     1021 aagtcgaccg ggaaaagaac ccgtcggtgc cgaacaacca ggtttacagg caggaccctt
     1081 ccggcgaggc ggatcaggac tgcaacttcc tcggcttctc ctgttctaat ccgaccgtca
     1141 ccctgaccgt cagcggaggc cccggccaga ccgaggtccc cgacgtaacc ggcctcagcc
     1201 aggatgacgc cgagcaggag ctggaggatg ccgggttcac gaccagcatc gagacccgga
     1261 cttcctccga cgtggattcc ggcctggtga tcgaaaccga tccggccgga ggcgagatgg
     1321 cccggcgcgg ctccgaggtc acggtgaccg tttccagcgg tgccgcccag gtcaaggtcc
     1381 cgccggtggt cgggcagacc ctcaacgccg ccaagcagca gctggcggcg gtcggcctgg
     1441 attactcctc cagcgaagag cccagcgacc gcccgcaggg cgaggtgatc gaccagtcgc
     1501 cgaccgccgg gaccagggtg gacccgggct caaccgtcac cctggtcgtc tcctccggcc
     1561 cggaagacac ccgggtttcg gtcccggccc tggtcggcca gacccagtcc tcggcggaat
     1621 cgcaactgac ggccgccggg ctggtcccga gcgtccagac ccagagcacg acgatccagc
     1681 cgcaggacgg caaggtgatc gaccagagcc cgggttccgg gaccaaggtc gcggaaggtt
     1741 caaccgtcgt gatcacggtc ggcaagtacg aaccggacag cggcaccacc ggcagcgaca
     1801 ccggcggcat cggcatcgac tgacccgtcc gccgacccgc tcgactgccg accaaatatc
     1861 gttcccggca gatgagagtc gcagtcctca gcggtggccg gtccagcgaa catgaaatct
     1921 cgctgctttc gggcgaggcg gtcgtggaag gcctcgaagc ggcgggtcac gaggtcatcc
     1981 gcatccagat cgagaagtcc ggtctttggg tcagcggtgg ccaagcggtc gagatccgcc
     2041 ccggcgaggg cttgaccgtg gacggtgacc tgctttcgct cgatgtcgtc tttccggtcc
     2101 tgcacggacc tttcggtgag gacggggtaa cccagggagc gctcgacacg gtcggtgttc
     2161 cctacgccgg ctcggacgtg ctcgcctccg cggtctgcat ggacaagctc accctgaagc
     2221 ggctttgcgg tttccacggc atcccccagg tcggtttcgt ccaggcgttc actcccggct
     2281 gggaggagga agcgctgggg ttggagttcc cgctctgggt gaagccctcg cgcctcggtt
     2341 cgagtgtcgg aatcagcaag gtgaccgacc cggccgggct gaccaccgcg gtcgaggagg
     2401 cggcgaggtt cgaccctcgc gtgatcattg aagccaactc cgccggacgg gagatcgagt
     2461 gctcggtgct cggaaacgag aacccggaag cctcgctgcc cggggagatc ctcaccgatg
     2521 acgagtggta cgactacgaa gccaagtaca ccgagggccg gaccaggatc gccgccccgg
     2581 ccgacctcga tgaaccgacc acggcggcgg tccgtgatct cgccacgcgg gtcttcacga
     2641 tggccggctg ctccggcctc gcccgctgcg acttcttcgt cgaggaacca gccgccgggg
     2701 aatccggcca ggcccgcatc ctcctcaacg agatcaacac gattcccggc ttcacccgaa
     2761 cctcggtcta cgcaaagctc tgggaagcca ccggcctccc ctacccggac ctctgcaacc
     2821 gcctcgccga actggccctc gaccgccaca agtccgagtc cgactacacc ttctagcccc
     2881 accccaaccc ccggcgaccc aaatccccac gcttcaaacc cgcgcgactc aaaccgacgc
     2941 gaccaaaccc ccacgcccca ggttgcccgt cgctggccac ccccggggtc accactggca
     3001 gaggtgccgc aaatccagcc ccttgatggg gcgaaacgtg cggcgcctcc agcctcccga
     3061 cggggcgaaa cgtgcggcac ctccagcctc gtgacggagc gaaacctgcg gcacctcccg
     3121 atacgggtcc gatccgctcc catcatgaac cggtttctgt tgcagaattg ttgctcttgt
     3181 cgagccacat gtgagcattt cttgctcatg ctccagggat ggatcccaga tggacgggca
     3241 tcgagagtga actgaaccgg cagatgaggg aggtcgccct gactcatgac ggtgttctga
     3301 ctgccgaaga cctaacggcc atcggtctga ctcgcaagat gattcagaca aggaaggagt
     3361 ccggaaggtt gattccggtc ctcaaggggt ctttcgccct gccaggaacc cggttgagac
     3421 ttcgaggccg gtgtcgcgcc ggggtctgtt cggcaggtga gggggcagtt gtctcgcact
     3481 cgagcgcgtt ggcacttcac gatgttgttc gcgatcccgg ggtagttcac ctcaccggcg
     3541 tttcgagagg gtctggcggc acccgatccg gcagatggtc cagcgacaac tacggcttca
     3601 aggtcatacg gcacgaaacc cgtcaacttc cagacagcca gatcaccgtc gtgaatggaa
     3661 taccgacgac cacggtggag cgagcactac gcgatttcgc ggccgaagca actccggccg
     3721 aaatgacaaa ggcattgacc cagggagaga aggaacgggc aatctgctgg ggcacccttc
     3781 gcgagctggt ggcagattcg gccggacaca aggggaacac gatgctgtcg accgagattg
     3841 agggatggaa tccagccttt gtcgatacgg gctccgatcc ggaaatcgac ttccttcgaa
     3901 tgatccgcaa gcgggaaatg ccctggcccg cggtcaacgt gaagctgggc cacaacgtcc
     3961 cggacttcct ctggtggcat ctacggctgg cggtcgaact ggacccgtac ggaacgcaca
     4021 gcggagcggg atctcaccgt cgcgaccatc gcaaggggat tgaactcgaa accatgggcc
     4081 tgcgagtagt ccgcttcacg ggcgaagacc tctaccggca tgaagaaagg accggtgacg
     4141 aactctggat tgtcattcga cagcagtccc ggctccttcg atgcccgatn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna gccgtagagg cggatgtcgc tgatggcgac
     4321 gccgaagcct gaatcggttt cggcggggtc ggtgatccag aggaggtagt actggtacct
     4381 ggtgccggcg gtgatcaggt cgatctgctg ctgttcggcg acgtcgggga cctgggcgag
     4441 ccgggtccag ccggaaacgt cgtccgggat cggatcgttt gacccgtaga cctccagggt
     4501 ccagcccggg gtcggggtcc ggatctccat ctccgaggcc tcgaccggtt cgcccgcgtc
     4561 gacgtacatc ccgacccctt ccttgtcccc gaagctgtcg gtttcatagg tctcggtggt
     4621 ccaggcgctg gccgaacggt catcgtcgat cgcgtaatcg atctcctcgg aatgctcctc
     4681 ctcgtcgccc tgcgggtcgt agtcactggc cccgaccagg gcgatctggg tttcgttcga
     4741 cccgccgcct ccggccccgc cgccaccgcc gccgccggtg tcaccgcggg agatgaagta
     4801 agcggtgccg gccgcgatca cgagggcgac cagcagcatc aggacggccg gccagggcga
     4861 ggtctttgag gtcgggatct gccggcgggg aggggcgacc gcatcgagca ccgaggttgc
     4921 ctcaccggtg gtgccgccgg cccggatcgc ttccacttca agcgcggcct gaaggtcatc
     4981 gatcatctcc tcgatcgtct ggtagcggtc atccgggttc ttggcggtcg cccggtcaac
     5041 cacccgggcc gaggccgccg agatgtccgg ccggatcacc tgcacgtccg gcaactcctc
     5101 gtttacgtgc ttcatcgcga cgccgatctg gttatcggcc tcgaacggca cgtcgccggt
     5161 cagcatctcg tagagcacga tgccgagtga gtaaatgtcg gagcgagggt ccacttcctt
     5221 gcccatcgcc tgctcggggg ccacgtaatc ggtggtgccg atcacccgac cggtcgcggt
     5281 gacgccctcg tcattcagtt cccgggcgat gccgaagtcg gtcagcttgg cccggccggt
     5341 cgaatcgatc aggacattct gcggcttgac gtcgcggtgg accatgttgc gctcgtgggc
     5401 gacgccgagg ccctgggcaa cctccagccc gtaggccagc gcctcggtgg cgtcgagggc
     5461 accgacccgg gcgatccgct gtttcagggt ttccccctgg acgtactcga gcacgatgta
     5521 gggccggccc tggtcctcgc ccgcgtcgat cacggcgacc acgttcgggt tggaaagctt
     5581 cgccaccgcc cgcgcttcct ggttgaaacg ctcgagctga tccggctgct cggtcatctc
     5641 ccggtgcatc agcttcaccg ccaccgggcg gtccagcacc tcgtcgcggg cgaggtagac
     5701 cgtggacatg ccgccactgc cgagcttggc ctcaagccgg taccgatccg aaaggaccgt
     5761 cccgatcatc tgtgagctgg caggcgggtg gttcacttca ctgattttgc ccgctcaagc
     5821 tcataggcac cggaggctaa ccgcaggcac cggtttccgc agcgggactg acaaattcgg
     5881 gaaaaattgc tcaattcgct tcacggtcca gtatgaccct ctcgcaggag gtccgaagtg
     5941 ttggtttctg ccaatcaaaa cccggcgaag ggctgacaga gcggatttgc gaagtgccgg
     6001 cgccggggga tcagtcagcg cgacggatga cctgccggtc aagggtcaac gtgtacgccg
     6061 gacgacccta cggtcgccgg ccgcgacgtc accgatccgg cgggcttccg ggtaatgacc
     6121 ccgcagcact tcgagggcgg cagattcatc ttcggcggga accaccacgc agaaaccgca
     6181 gcccatgttg aagacctcgt acatctcctc gtccggcacg ccggcccgct cggcgatcag
     6241 ctcgaagacc ggctggggag caagcgggtc ggtgatctca taggtgacct cggccttcag
     6301 ccggagcagg ttgtcgagac cgccggaagt gatgtgggcg aggccgtgga cccggaccgg
     6361 ggaggcgagc aactccagca ccgccttgac gtagatctcg gtcggctcga gcaggatctc
     6421 gccgagcggg cggccgagcc ggtcgtcatc catgccgatc ccgtccaggg cggaacgggc
     6481 cagggtgtag ccgttcgaat gaatccccga ggagggcagg ccgatcacca cattggtcgg
     6541 ggcgaccgcc gatccgtcga tgatcgagtc gagggcgacc gtgccgaagc aggcgccggc
     6601 gatgtcgaaa cccttgacca ggtccccgat ctgggcgagc tcgccgccgg ggatctcgat
     6661 tccggccagt tcggcgcccc gggccaggcc gaccccgatc tgctcgcaca tctccgggtc
     6721 ggccttctcg accgcgaggt aatcgagcat ggcgaccggt tcggctccga cgcagatgat
     6781 gtcgttcacg ttcatcgcga tgcagtcgat cccgaccgtg tcgaaacggc caagttcctc
     6841 ggcgaccagc agcttggtcc cgaccccgtc cgtcgaaagc gcgatcccgg tgcggttgtc
     6901 gagccgcatc accgcggcgt aatggccctt gagatcgacc tgccgggtgg ggcggccgat
     6961 gtttgcggtg gccagggcgg cgaccagccg gctgaccgcg aggtccgcgt cggactggct
     7021 gactcccgcc ttcgcgtatg cgtcttcgcc gctcatcgca gttgattcaa cattagaagg
     7081 cggtcactcg gcagcacggt caccgcgatc ggcggccagt ctcgccagga tcagcagggc
     7141 gaaagcgatc cgataggccg cgtaaggggc gagtgcccgg tccctttcaa ccatccggat
     7201 cagatcggtc gcggccatcg tcgaggcaaa ggaggtagcg gtgccgatcg ccagccagcg
     7261 cgcctgccgg cgtgagatgc cgcgccgggc aagccgtacc cccttcaggc cgaccgcccc
     7321 gaggatcacc ggcagggcga cggtgcggga gagcagattg gcctgatcac gggagaagcg
     7381 gcgcaaccgg gcggcggtca gggtggcgcc gttgcgtgag acgccgggaa tcagcgccgc
     7441 cgcctggccg atcccgaggg cgagcccgtc cttccagtcc gcttcccccc ggccccgctc
     7501 ctggggccgg cgatcggcca gggccatcgc cagccccccg aggatcagcc cggtcgcggt
     7561 gccccgcggc ccgccgaagc gggattcgat gacccgttcg aatccataac cgacggcagc
     7621 cgcaggcagg aaggaaagga gcaggaccga ggcgcgacgg aggtcaaact caccgatttc
     7681 atcccggatc tctccgcgcc gggtgatcag cagcgccagt gctgcgcccc cgtgaagggc
     7741 gacttcgaag ctcttgcgca gttccgggtc gagctcatcc ggatcccacc ggagcaggcc
     7801 ggggagcatc gccagatggg ccgaggagga gatcggcagc agttcggccg gaccctgaac
     7861 cagcccgagg accaccgcat ggccagttgc gcgtcggctg tcaccggaat cggaaatgag
     7921 agcggggatg ctacccgcgg ggtagaatcc gtggcttcaa tgcgacgccc agactttcat
     7981 ttgcgccggt accagctgat gcagatggcc ggtgacggcc tgctcgtcgc gctttcctac
     8041 ttcctcgcct tccagcttcg cttcctcgat gacccgggga cgatccccga gcgttacgtc
     8101 gacctgttcc tgcagtcggt cggtttcgtg atcgtcggca agctgatcgt cttctacctg
     8161 ttcgggctct accagaagtg gtggcgtttc gtgggcggtc gtgacatcgc ccggatcgtc
     8221 caggcggttt cggtttcgag cctcctgctg gtcgtgctgt tcacggtgat ccagcccttc
     8281 ccgcatgacc tgccgcgctc ggtcgcggtg accgactttc tcctcaccct cttcctgatc
     8341 gccggcgccc ggctcgccac ccggctcttc aaggaacgcc cgaacaaggg caaccggatc
     8401 gcccggggcc gcgaggtgct ggtggtcggg gccggttccg gtggtcagat ggtggtccgt
     8461 gaactgcgga tcaacccgaa cctcggcgag accgcgatcg gcttcgtcga tgacaacacg
     8521 gagatgcgcg ggatgcgcat ccagggcatc cgggtgctcg gcaccaccga cgagatcgag
     8581 acgatcctcg acgaaaccga accggacgag gtgatgatcg cgatcccctc cgcgcccggt
     8641 gtgctgcggg cgagggtggt ggccgcctgc cgtgaacggc agatccgggt ccgcaccctg
     8701 ccgaccgtgt tcgagctgct gcggggcggc gtccagctaa gccagcagct gcgcgaggtt
     8761 caggtcgagg acgtgctcgg ccgcgatccg gtgaccatgg agctggcccg ggtcggcgcc
     8821 tacctcgagg accaggtcgt gatggtgacc ggcgcgggcg gctcgatcgg caaggagctc
     8881 tgccgccaga tcgggctggt cggaccgcgg ctgctggtca tgctcgacca cgccgaggac
     8941 aacctcttcc agatcgaacg cgagatggtg gccgagcgcc acttcaccag ggtcgaggcg
     9001 gtcctggccg actgcaagga gaccgagcgg atcttcgagg tgatgcagcg cttcaaaccc
     9061 aacatcgtct tccacgccgc cgcctacaag cacgtgccgc tgatggagtc gaacccgctc
     9121 gaggcggttc gcaacaactc gttcgcgacc cggatcaccg ccgattgcgc cgccgcggcc
     9181 ggggccgagc gcttcgtcct gatctcgacc gacaaggcgg tcaacccgca gaccgtgatg
     9241 ggtgcctcaa aggcaatggc cgagtgggcg gtcgccgccg ccgacgcgca ccacgcgaac
     9301 acgcgcttcg tctcggtccg tttcggcaac gtgctcggat cctccggcag cgtggtgccg
     9361 atcttcaagc gccagatcga gcagggcggt ccggtcacgg tcacccaccc ggagatgacc
     9421 cgcttcttca tgacgatccc ggaagcggtc cagctggtca tccgggccgg cgacaccggg
     9481 gtcagccggg gtgacgtctt cgtgctcgac atgggcgagc cggtcaagat caccgacctg
     9541 gccgagaaca tgatcaagct ggccggtcgc gagccggaga aggacatcgc gatcgagttc
     9601 accgggcccc ggccggggga gaagctccac gaagagctgt tcggggtgga agagcggccg
     9661 cagccgacgg cttcgaaacg gatcatgagg gcggtcaggg aggaaccgat cgacccggaa
     9721 tggatcgttc agaccctcga tcgactcgat caggtcgttc agaccgggga tgaagcgaac
     9781 ctcgccgaga ccgtggtcga actggccagc aaccccggca gcgatccggc aaaggtcatc
     9841 tgaaaccggc tcaaggcgca ttcatccgcc aaacccaaat aacatgcccc ggtcggcgta
     9901 tccgggtcgt gggcccacca gccccgggaa ctacgccctg acgtccagaa aagtggagat
     9961 cagttgaccg acgtaatcca gaccgtaggc tcctacgccg ggctcgtcgc cctgctggcg
    10021 attggcgtgc tcgctttcct cgtgttctcc caggccaggg acatccgccg tctgcgcgaa
    10081 tgggccggcg gtgcaccaga gcgggatgcc gaggttcgcg aggtcagcga gatcgtcgcc
    10141 gaagagcgct cggccgagct cagggtgctg gccgaccgcg aggagcggcg catggagcgg
    10201 gccggactgg ccgagccttc cttctgggat cgcctcggcc gcagcggccg gatcatcgcg
    10261 atcgtcgcgg cggtgctgat cctcggcgcg gctgccgcct gggctgcgac cagcctgctc
    10321 ggctcggaca gtggcaactc cggcaacggc aaaggcggca acgcggccaa gtcgcagaac
    10381 gccaaacccg cccagatccg ggtcgccgtc ctcaacggca ccggtggcac cgaatccggg
    10441 ctcgccaaca cttacgccac tgccctggag gacgacgggt accagatcgg tgtggtgacg
    10501 gacgcaaccg acaccttcac cgagagtgtc gtcatgttcg cgagcggcca tcaggcagaa
    10561 gccaggaagg tcggcaaggc ggtcgggatt gacaacacgg ccccgatgga cgccgccatc
    10621 tcgggacttt cttccggtgc cgacgtgacc gtggtgatcg gcaccgacca cggccccctg
    10681 cccggggagt aaatggataa ccccgccggc ccccggggcc caggcacgac tcgcctggga
    10741 tggctggcgc cactcacgat cggcctgctt ctggtggcga cggtggccgc cttcggctgg
    10801 tcgcagcggc tcaagcgtga gccgctggtg atcgaccggg tcgagtaccg ggtgctcggg
    10861 tacacgccga acggtgaatc gccgaccgtc ttcagcccca acggcgattg ccggcgggac
    10921 cggatgtcga tccacttccg cacgaccagg tcggacaccg ccgacgtcga gatcatcggc
    10981 ctcggcggca ggaccgtccg caccctggcc cgcgaccgct tcttcaagcg ctaccgcgag
    11041 cacacgctga tctggaacgg tcgcaaggac gacggcggga tcgcattcac cagcaagtac
    11101 cgggtccggg taaccatgca cggcgaggac cgggtcctct acctgcccgg cttcatccgc
    11161 ctccacaggt acgcgccccg cggaccctcc ggttgcccga agccgctcaa gtggaagtac
    11221 tcggtcaagg aaccgggcaa accggcgaga cccctgacga gggccgaggt cgcgaagatg
    11281 accagacccc tgaccgaggc cgaggtggcg aagattaaag ccgcggcgaa gaaggcgctg
    11341 ggcaccggcg gcaccaccgg agggcagggc cagtgatcga ggcgctcgca gccctcgtcg
    11401 ccgcggccgc gctggcggtc gcgatcctcg accagccttc gatgcgtcgc tcgatcgcga
    11461 tggcggtcgc cggaatcatg gtcccggtcc tgatcgccgg tgaccagtgg cattccggcc
    11521 cgatcaccga acttcgaaac tcaccggcga tgctggtcct ggccctgctc tgcgccctgg
    11581 ccgtggtcgc cctgatcgcc tgggtggtcg atcgctggcc ggtcatgctc ccggtgctgg
    11641 tcctggtcgc gctgcccttc cggatcccgc tgaacgtcgg cggggaggat gcgaacctgc
    11701 tggtcccgct ctatctggtg atgggcggcg gggtgctggc ttcgctcatc cgggcgcgtc
    11761 ggaacgacct gctgcccgcg ttgccgaccg ttgccccggc cggtctggcc cgctggctca
    11821 aacccctgct cgccgccgcg gtggtgctct acgccctcgg cctcttctac acggatgacc
    11881 gttcgaccgg cctccagaac ctctgtttct tcctgattcc cttcggggtg atcttcgccc
    11941 tgatggccga ggtcgagtgg accagggacc gcctgcggat cctgctcggc gtggtcgtcg
    12001 gcctggccct ggtctgcgcc ctggtcggct tcgtggaata cgcgacgcgg gacctgctct
    12061 ggaaccaggt cgtgatccga tcgaatgact tccacgtcta cttccgggtc aactcgctgt
    12121 tctgggatcc gaacgtctac ggccgctacc tggcgctggc gatcaccatg gtcgccgcgg
    12181 cactgctctg ggcgaagccg agccgcgagt cggtgatcct cgcggtggtc gccgccgtcc
    12241 tctggctcgc cctgatgacc acctactcgc agtcgagctt catcgccctg atcgccggcc
    12301 tggcgaccct gatcgcgctc cgctggagcc tcaaatgggt tctggccggg ctcgtcgcgc
    12361 tggccgtcgg ggtgatcctc ttcggcacct tcgccggcgg cctggtcaag ctcgacctcg
    12421 gcgcgatcaa caagcagacc agcggccggg cgaacctggt cgagggcgga atggacctct
    12481 tcgaggccag gccgctctat ggctacgggc ccggttcctt ctcggttgcc ttcaagcggg
    12541 aggtggccgg accgaacgcg ccggtgaccg aatcccacac cgaaccggtc accgtcgcgg
    12601 ccgagcaggg gctgatcggc ctggccttct accttgcgct gctggccagc atccttgccg
    12661 cgctgctggc cggaatgcga aaggtgatgc ccggcctcgg tgccccgccg cgctcgtacg
    12721 acccggaccg cggacccccc gccgccaggg ccgccatcct cgccgccttc gtcgcggtgc
    12781 tggttcacac gctcacgtac gcaggatttc tcgacgaccc ggttacctgg gtcctgatcg
    12841 ccatcggcta tagcctcgcc tttccatgtc gggctacctc cgccgcctag cgacaaccgg
    12901 cgccgcctac acggcggcca gcatcatctc gaagctgatc gcggtagccc tgttgccgct
    12961 ttacacgcgc tacctgaccc cggctgatta cggcgccgcg gaagtcatgt tctcggccgt
    13021 ggtcgcggcc tcgatcatca ttcggctcgg cgtggtcgag gccctgctcc gcttctatta
    13081 caagcagggt gaggagccgg ccgccgtcgt ctcgacttcc ttcgccaccc tgttctgggc
    13141 ggggctgatc tcggtcatcg tcctgatgcc tttcgccggg ccgatctctc agctcctcct
    13201 cggggaatcc gaaccgaccc tgggccggat cgcgatcgtc ggcctcttcg tgctcaccct
    13261 ttccgaatac cttctgacca tcttccggct ggacgagcgg gcccgggcct tcttcacggt
    13321 gaccatgctc tcggtcctga tcacgatccc ggtcaccgtg atcctggtcg tcccgctcga
    13381 cctcggggcc gaaggcctcc tggccggcag ctacggcacc ggcctcgcga tcgtcctcgg
    13441 cctgatcttc tacaaccgcc gtcggctcgc cctggccccc gaccggccgc tgctcaagcg
    13501 gatgatggcc ttcggcctgc cgaccatgcc ggccgagctc tcgctctact cgctcagctt
    13561 catcgaccgc atcctgatcg cccgcacgct cggtctggcc gaggccgggc tttactcgat
    13621 cgcgatcaag atgtcccagg ggatcgcggt cctggtccgc ggtttccagc tcgccttccc
    13681 gccgctcgcc tactcgatcc aggatgacga cgaggcccgc ctcgcctact cggcggtggt
    13741 cacctggttc gtcacggtga tggccttcgt ggtcagcttc ctctggctga tcgctccctg
    13801 gatcgtcggc ctgctcgccg cccccgagtt ctccgaggcg gccgtcgtgg tcggaccggt
    13861 atcggccggc gccgccctct acgcgctcta catggtgctg ctggtcgtgc tcggccggac
    13921 cgggcgcacc gagtacaacc tgccggcgac gatcgccggg ctggtcgtca atgtcggcct
    13981 caacctgctg ctgcttccgc tctgggggat catcggggcc ggggtcgcgc tggtcgtctc
    14041 ctacctggtc gtggtggtgc tgatgtacct cttcacccag cgactcttcc cggtccccta
    14101 cgagtggctg cggctggtcc gggtgctgct cgccaccgcg gccctggtcg cgatcggcga
    14161 ggtcttcgtc ccggatgacg gtctcgcctg gctggcggtg cggctgctgc ttgttctcgc
    14221 gctcccgttc gctctgctcc tcacgggctt tttcaccgcg gaggagcggg ggtggttgct
    14281 cttgttgacg aagcccggtg agctgaaggc gaagatcctc gccgcacggg aggttggaaa
    14341 agcggcgggc gaggagggtg aaggcaccag cgcctcaggc aggcccggga gtcgcgggtt
    14401 gccggaggcg gttgaggtcg aacagtggga cgaggacctt cgttcctaga ggtgccgcgc
    14461 atttaggacc gtgatggggc agaacttgcg gcacctctcc cggaactcag gattccgggc
    14521 tgccggcgcg gtcggcgagg ctttctcccc ggtaggggaa gagggcctgg cgggtgtggg
    14581 cgcggtagac cggggtgggg gcgccgggca tgaagaccga ggcgatcgtc cgcaggccgt
    14641 aggcccaggc ggtcagcagg cggaccgtga aggcggcggc ccggttgtgg tgctttcgca
    14701 tgtagaggtc gcggttgcgg tggaactcga cgatgcgggg caggccggaa gagaggtcgg
    14761 tcgagagttg gttgtggtgg accgcctcgg ccgaagggac gtagagcgtg ctccagccgg
    14821 cgtcggcgag gcgtttgcag aaatcgcatt cgtcgtagta gacgaagaac tgcgggtcga
    14881 gcccgccgat ttcccgggcc gcttcgcggc ggaccatcag ggccgaggac tgggcccagt
    14941 cgacccgccg ggtttcctcg cccttgcttt cgacggtcag gcgcgagtgg aggaagagcg
    15001 cgccgacgaa agctgactcg accccgggaa agcgccaggc gcaggcgtac gcgttgcctt
    15061 cgccgtcaaa cagttgcgcc cccgccgccc ccgcttccgg ttcggcatcg agggccgcgg
    15121 ccagcgcccc ggcagcgcct gccctcagct ccgaatcctc gttcaagagc aggcagtagc
    15181 ggccgcgggc ccgctccagc agcagtgagt cgttggccgc cttgccctgc ttgctctcca
    15241 tggcgatcac ctctgccgag ggaaactgtt cccgcaccgc ttcgaccgaa ccgtcgctgg
    15301 atgcgttgtc gaggacgagt acctcgctct ccagtccggc cgggtggaac ttctcgatcg
    15361 cggccaggca atccatcagg tactcccggc cgtcgttgtt taccacgcag tagctcaggt
    15421 ccacttctgt ggcttcggtt gcagggtcgg gtgccggaga ggggctgttc acggttgcca
    15481 acgataatcc cggttgtgca tgtcaagctg atcgacccct cggctttcac cccgccgtac
    15541 gaccggtcgc tggctgctgc cctggcggcg gaaggcgccg aggtcgagct gatcacctcc
    15601 gaattcacct acgggccggt gccggaaccg gagggttacg aggtcagcct cgacttctac
    15661 cgccgcattt cgccgcgggc ccgacggttc agcaaggcgc ttcagcactt cccggagatg
    15721 cgccgcctcc ggcgcaggct ggagaacgcc ccggacccga tggtcaccca ttaccagtgg
    15781 ctgaccatgc cgcggttcga tcatcgactg atctcgaagc gccgtccccg tttgatgacc
    15841 gcccattaca tcctgccgcc cggcccgaac gggaagcagg ccgcggccgc gaagagggtt
    15901 ttcggcgcaa tggacgcggt gatcgcccat tcgaagggcg gggcgagccg actggtcgaa
    15961 gaggtcggcc tgccggagga gcgggtgcgg gtgatccctc acggcgcctt cgactacctg
    16021 acccggctgc cggaggaagc cccgttgccc ccggaactgg ccgactccgg tgccgaggct
    16081 ccggtcatcc tcttcttcgg cctgcttcgc ccctacaagg gaatcgacgt gctgatccgc
    16141 gcggccggac agctggccgc ggatgccgac cgagacgggc ccgctcctga gctttggatt
    16201 gtcggcaatc cgcgcatgga cctgaccgaa ctggtcgaac gctcgaggaa gctgaaggtc
    16261 aaggtccgct ggctgccccg cttcatcgag gatcaggaga tcccggcgat catgcgacgg
    16321 gccgatgtcg tcgtcctgcc atacctcgat ggcgagcagt cgggtgtgtt gtacacgggg
    16381 atcgccttcg gcaaggcgat ggtcgtctcc gacgtgggcg gtatcggcga ggtcgcccgg
    16441 gaacacgggc ttgcccggac cgtcgagccg ggtgatgtcg accagctggc cgccgagctt
    16501 tcggcgttgg tcctcgggga ccgggccgct gcggccaggg aagaactcga gacgaggtgc
    16561 agggtcgcgg cggagggacc cttcgcctgg gccgccgttg cccgacagac gatcgcgctc
    16621 tacgaggacc tgctcggccg ggggagtgga tcgtgatcgt cgtctacatc ctcttctggc
    16681 tcagcgcggg cctggtcttc ttcacccaca tcggctaccc gatcacgctc tggctgctcg
    16741 acttcatcgg cctgggcggc cgcgccgggg aggtgcgggg ccgcaagctc agcgagattc
    16801 cgccggcaat cgatcccgac accgaggagg agattctgcc ttcggtctcg ctgatcatcg
    16861 ccgcctacaa cgaggagacc gtgatcgagg cgaaggtgaa gaacgcgctg gagcttgagt
    16921 atccgcgcga gctccttcag gtcatcgtcg cctcggacgg ctcatcggat cgcaccgtgg
    16981 agatcgcccg ggaggccggc gccgaccttg tcctggatct tccccggggc ggcaagatcg
    17041 ccgcgcagaa cgctgcggcg gaagcggcga ccgggaacct cctggccttc tcggatgcga
    17101 actcgacctg ggagccgggc gcgctcgccg aactgatcga ccccttccgt gacgagcgag
    17161 tcggatatgt ctgtggtcag gtccgcttca tcggacccga cggcggcaac ctcgaaggcg
    17221 cctactggcg ctacgagatg cgggtgcggg agatggagtc ggagctggcc ggggtcaccg
    17281 ccggaaacgg cgcgatctac gcggtccggg ccagtgccta catccccctc gccccctcgg
    17341 gcagccacga cctttcgttc cccttcgccc tggccaagcg aaggctcgct tcgctttacc
    17401 ggccccgggc ccgggcaacc gagaagctgg tccccagcct cgacggcgaa ctggcccgca
    17461 agcggcggat gatggtcggc ctctgggaca tcgtggtcgg ggagggaatg ctcgacccgc
    17521 ggggctactc gccgctctac ctcttcgaac tcttctcgca ccgactgctc cgctacctca
    17581 gcccggccct tcacctgatc gccttcctga ccaacctcgc gctgctcggc cacggctggt
    17641 tttacgggct gaccatgctg cttcagctgc tgctcgtggc cggggcgatt ctcggtcgct
    17701 ggatcccgat cctgcctttc cgcatcagtc gctactacct cgaggtgacc ctctcgatcg
    17761 cgctcggcct ctgggaccgc tggcgccagg gcccgccggc cgcctgggag aaagccgagg
    17821 gcaccaggtg agcatgccgg gcgacaggat ggcggtggct cgttgagccg gggcttcgaa
    17881 aaactggtca cggcgctcgt gctggtgatc accgccccgc tcctggtggt ctgcgcgatc
    17941 ctgatccggc tcgagggcgg cggctccgcg atctaccggc agacccgggt cggcctgcac
    18001 ggtgaagagt tcgagatgct gaagctgagg acgatgatcc ccggctcgga tccggtcggg
    18061 gttggcaccg tggtcggccg ggaggacccg cgggtgaccc gggtcggccg ctggctgcgc
    18121 cgaacctcgc tggacgagct gcccaacctg gtgaacgtct ggcggggtga gatggccctg
    18181 gtcggccccc gcccgacgat cccggcccag gtcgccgatt acacgccgca tcagcatcgc
    18241 cggcacgagg tccgccccgg gatcaccggc tgggcccagg ttcagggccg ggccgggatt
    18301 ccctgggacg aacggatcga gcttgacgtc gagtacgtgg agaaccgctc gctccggctc
    18361 gacctggaga tccttcgccg caccgtctgg ctggccgcga ccggccaggg cctcgccccg
    18421 gactgagcgc ggcgatcatt taggctttcc ggatgcgcat tggccagata atcggcgcga
    18481 aacgccccgt cttctcggtg gagttctttc ccccgaagac cgacgagggg cgcgcccagc
    18541 tctttgacac cgcccggcaa ctggccgaac tcgaactcga tttcgtttcc gtgacctacg
    18601 gggccggcgg ctcgacccgc gagggcacgg tggacatcac gaccgctctc aaggacgagg
    18661 tcgggctcga ggtgatggcc catctgagct gcgtcggtga gaccgcggat ggcctcgacg
    18721 cgacgctgga acggctccgg gatgccggca tcgacaacat cttcgccctg cgcggcgacc
    18781 cgccccgggg tgaaaccgac ttcgtccagc ccgagggcgg cttcggcagc tcggcggaac
    18841 tggccagcca catcaactcg aagtacgact tctcgatcgg cggggcctgc ttcccggagg
    18901 tgcatccgga agcgccggac ctcgacaccg acctgaccta cctcaagacc aaggttgacg
    18961 ccggggccga gttcctgatc acccagctct tctttgacaa ccgcgcctac ttcgacttcg
    19021 tcgacgcggc ccgggcgaag ggcatcgagg tgccgatcct cgccgggatc atcccggtca
    19081 ccggctacgc ccacaccaag cggatctgca atctctgcga cgcgagcatc ccggtcgagc
    19141 tcgaggcggc gatgctggcc gccgagggtg actcggaagc cgagttcaac ctcggcgttg
    19201 cttacgccgc ccagcagagc gccgaactgc ttgcggccgg tgccccgggc atccacttct
    19261 acgcgctgaa caaggccccg gcgacccggg ccgtgctcgg tgctcttcgc gccgcccagc
    19321 cctggcgcgg cgcccgcggc cagtcgctga ccagctgacc cggtcaactt gggccaggaa
    19381 ggcaacaggg aggtaaagca gccgaccagg gccggttacg cgatcggcgc cgtctttctg
    19441 ctcttcatcg ttggcggggc cttcttcgcc ctccgttcgg gcggggacga tggcggcgag
    19501 gctcacatct ccaccgcctc cggttccagc aacgggctgg ctccggacac ccgcaagggc
    19561 accccgtatg aagggccgct gatcaccgat ctggccaggg ccgcggcagc cgcgaaatgc
    19621 gtggtccgcg agggccttcc cgaggaaggc cacgaccaca tcaaccgggg tgatccggtt
    19681 cccgaataca ggacggtgcc tcccacctcg ggcgatcaca tcgtgccgcc gctccagcag
    19741 gccgacggcg cctgggccga cccggtggcc ccggtggacg taattcactc gctcgaacac
    19801 ggccgggtcg cgatccagta ttcgccagcg ctgccggaag aggaccagct cgcgctcaag
    19861 ggcctctttg actcggccta ctcggccgcg ctgctcttcc ccaacccgga gatgccgtac
    19921 gcggccgccg cgacttcctg gcgcaacctg atcgcctgcc cccggtggca gggccggaag
    19981 accctcgacg cgatcaacgc cttcggcgtc gcccactggg gccagggccg cgaaccaatg
    20041 gccggcttcc ccgccttgag cgggccctcc ttcggggacc cataagtcgg gcggggtccg
    20101 acccgccctc catgtggaac ccgccggccc attgggctca aggatggttg ggccggctct
    20161 ggcttctgta cctcgggaag aaatccgagt cggcgttcgg ggcgagtctt gccgtcagtg
    20221 tttcgtaggc cgtgccgtcg cagacgatct cggaggtgaa ccactgtcgt tcggtgttgg
    20281 cgaagcctcg tttgaactct tccagcgcgt cgcccggggt gatgccgccg ccgaagttga
    20341 gggcgagctc ccgttcggcc gcgaagccga tcatctcctc gaccacgttc ttcatcggcg
    20401 cgcccctgag atgggcgtct gcggtgcccg acaggtagta gtggaggagg ccgtcgctga
    20461 gcgcggcgat cgaagcggcg gcgaccggcc gcctgccatc ggcatccgcc tcctcttccg
    20521 gcgcgcggag caggaagagc caggttccct cggccgaaag gatccggtcg aagtacttct
    20581 cgctgaagaa gtaccgatcg gccgcttcgg tgcgggccat cgtctcggtg taggcggtaa
    20641 agaagccggc ccgctctccc ggcgtggtct ccggccccgg ggtgatctcg agcgagcaac
    20701 cacgcttcag gttcttgcgg atctgctgcc ggtccgaggc ccggctcttg cggggcaggg
    20761 ccggatcgga aagcaggcag acgttgcggg gagtggcccc ggccaggggc gggcccgcgg
    20821 cgagcgggtc cctctcttca agaggtgccg cacgttctgc cccgtcacgg tccgaaacat
    20881 gcggcacctc cccggagggt gcctcgagcg agtgccggag gaagatcgtg accaggcccg
    20941 tcccggtgaa atcgatcgcc tccgggtcga gcgggaagtc gagccggagc agcggcccgt
    21001 cggagatcgc gaggccaggg tatccgtagg gcgagatcgc gtcgagccgg tcgcttccgg
    21061 ggatctcgcg gacgatcagc ggggtggagg cgaccagttc ggtttcgtcg ccgtcctcga
    21121 agatgagcag cgagtgggta gtgccctcgg cttcgagaaa ctcggccgag cggaagaagt
    21181 cgggtccgct ggcggcgcgg ccaccatcgg cgaccagttc cgcggcgagc ctcaactgaa
    21241 ccccggggcc acgctcacca cttcccgact gcggcgaaga actgttccgg cgtggcgccc
    21301 cagatctcgc agtcgagcat ggtgccgtcc ggccgccgga tgtggccggg catgttgccc
    21361 tcataggtcc agccctgtgc ggtcacgacc ttcttcgcgg cttcgttgaa ggccgggatg
    21421 cgaccgtaga tccgg
//
LOCUS       DAOQKN010000016        21247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_1079_length_21247_cov_6.038128, whole genome shotgun sequence.
ACCESSION   DAOQKN010000016 DAOQKN010000000
VERSION     DAOQKN010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 21247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21247
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1079_length_21247_cov_6.038128"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="PLJ64_02150"
     CDS             <1..283
                     /locus_tag="PLJ64_02150"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HNC92328.1"
                     /translation="GPAASAAMKRLATGQQVTVTSDPTQDRRDHYGRLLAYIDRGSQD
                     LGYTLVKRGLASAYPYDGPFQRYPRYARANNHARTNNIGSWSHCDISPG"
     gene            complement(280..570)
                     /locus_tag="PLJ64_02155"
     CDS             complement(280..570)
                     /locus_tag="PLJ64_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HNC92329.1"
                     /translation="MTAGAGGFSRRIRVRLQPRASKNELKGWKEDPGSGEQVLQARVT
                     APPVDGKANQALIALLAKEFKTPKTRIRIIQGESARDKVVELAESDRDGHRP"
     gene            complement(574..2004)
                     /locus_tag="PLJ64_02160"
     CDS             complement(574..2004)
                     /locus_tag="PLJ64_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNC92330.1"
                     /translation="MSETPSGMAEQILAFCEELRSEDVAIGTSEIQDAFRALEIVPWT
                     EPVDFRESLATTLAKSPKDREIFDLVFDRHFFRATEAASLEFAPEGGEGRKAALSDEV
                     REELSMEALAEAIRQAIAEGDESAMNELARLAIEAFGRQGESSGVVGVDMQRIRRGLG
                     LSPSSDTSGDGEADGENLDRDQIARFERQLRRELQRQQIQKQGQLPPARPLSDLNRAL
                     PMRGAQDLAAVHKAVAQMKRRLATLGHDPRGAKRGRTVDMRRTMRSSLETGGVPLNLK
                     YRPRRPRKPEIYVLCDVSTSVSSASTFFLSVLHALHDSFRKLRSFVFIERISEVTHVF
                     EDERDFTAIAEKITATGGVADISGYTDYGRVWNEFYADVSADLGPRSTVIVLGDARTN
                     GRPPAEDAFARVADRANRTFWLNPEPELYWNYGDSVMSAYTPFCDGAFECWKTEHLEQ
                     FVDVVASGKVEHFGTTTRKYTSGHRY"
     gene            complement(2020..2952)
                     /locus_tag="PLJ64_02165"
     CDS             complement(2020..2952)
                     /locus_tag="PLJ64_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNC92331.1"
                     /translation="MAATPQAVPRSPAEVAEALRDASYLADDSTALIAFLAQRLGKPV
                     LVEGPAGVGKTELAKALSRATGRELVRLQCYEGLDEAKALYEWNYRKQLLQIQATAST
                     QDEGATPVGMGEIFTEDFLLERPLMKAIANPDPVVLLIDEIDKTDQEFEAMLLELLSD
                     FQITIPEMGQISATTHPIVLLTSNNSRELTEALKRRCLYLWLDYPARDREIEIIRLHA
                     PEIDEALAGRLVDVVDMVRELDLKKPPSIAEAIDWARTLILMGADDIDEKVFRESLSI
                     IIKHRTDLDLVAEKIVPRLGGSGSSNGSSANGAG"
     gene            complement(3001..3411)
                     /locus_tag="PLJ64_02170"
     CDS             complement(3001..3411)
                     /locus_tag="PLJ64_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92332.1"
                     /translation="MPPKPEQTEQQPGADRFWGQFSQSKAGRSSAENGSGAESAKAGS
                     EGPDGQGHGAECLEWCPICRSAELFRHTVSPELRQQAESIQHEAIGVLKAFLDAYADK
                     SGNSSPGGPSGPEPSPSDQPEEPATPRVTDIPLD"
     gene            complement(3395..4579)
                     /locus_tag="PLJ64_02175"
     CDS             complement(3395..4579)
                     /locus_tag="PLJ64_02175"
                     /inference="COORDINATES: protein motif:HMM:NF014433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRC40/GET3/ArsA family transport-energizing
                     ATPase"
                     /protein_id="HNC92333.1"
                     /translation="MPPRTILYTGKGGVGKTSVAAATARRCAAEGRRTVILSTDPAHS
                     LSDSLGVELGPDPVEVSPGLWGQEVLAEAEMRRNWDRVAAWMSGLLQSKGVDQVRAEE
                     LSVPPGLDELFSLLQIKRHWDKSEFDVVIVDCAPSGETLRLLGYPEVASWWVRKVFPW
                     NRSLLSKAAPIAKALEIPMPEPELMDEVEHLIENLLAMDGILRDHEHCSIRLVMNPDR
                     MVINEARRTFTHLCLFGYLTDAVIVNRVFPDEVADSYFAGWREQQATQLESVEEGFSP
                     VPVLTARYFDREVIGEEMHERLADDLYGEIDPSGLLHSGMPREVATEDGRTVIRILAP
                     FTEKGEVKLQQAGEELMVQVGEARRTIMLPSGLARRSPSKASFEDETLEIIFEDPHAT
                     QA"
     gene            4609..5229
                     /locus_tag="PLJ64_02180"
     CDS             4609..5229
                     /locus_tag="PLJ64_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92334.1"
                     /translation="MERTRIPLVLVLVLAFSLVMQACGSSDPDPATILDRALTREHLS
                     SFGNGSGGPTGGIVSVEALGYEDRVLDERKVAADPQVMSDIRSALGSDSGLRGLVGDL
                     RYDGTEEVSGVGTDHISGELDVAGLARALEAAGGGEVGQIAGVKTGSSLEDSLVSADF
                     DLYAGQQDGDIRRLDLTLALDDPDNALPPARIRFSLTPRPSGANQT"
     gene            5248..6276
                     /locus_tag="PLJ64_02185"
     CDS             5248..6276
                     /locus_tag="PLJ64_02185"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HNC92335.1"
                     /translation="MEYLLLLFSFAIILTGALLFTNAIEWLGHKLNLGEGAVGSILAA
                     VGTAMPETLIPIVAIIGGVSGSDEVAVGAIVGAPFLLATIAMALVGISALAYIKRRPQ
                     GKHLDVHVETLDRDLVFFMIFFTLALVVGVVDVPQSVQIGGGVICLLAYVYYVVQTIR
                     NSGDVAESHEMDPLIMDRRTDRTGGPPNSIMIIQLLVGLASMVGGAHLFVHELLSVAE
                     HLGVSAIILSLILAPLATELPEKANSFFWVRDGKDALALGNITGAMVFQSTIPVAIGM
                     IFIDWSLDKYAVLSMLLGLAGGVVAYWSLRRNGQFNKPSIAIWGLLYLTFLAFVILSG
                     DTGHSTAH"
     gene            complement(6478..7266)
                     /locus_tag="PLJ64_02190"
     CDS             complement(6478..7266)
                     /locus_tag="PLJ64_02190"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC92336.1"
                     /translation="MSNWDKGSYEVTAEQIAPATPAVADAVEPVTGKLALDLACGTGN
                     EALELARRGARVTGYDGAPRLLQVAGERAAAEGLELERVQGDLAELPFDDDSFEIVTS
                     VFGLIFTGSPQDSAAEIGRVLRPQGRIAFTSWVEEGLFQYMQDMAKAAVASHFGQAPP
                     EGADAPFAWGDEAAVRELFSESGLMLQVETRELVIEEESAAALNDRWFDLHPIWITMQ
                     EVIGDEAYEEMRREALPVVESHNEASDGSFRNTLRYLLFEGSPV"
     gene            complement(7259..7738)
                     /gene="bcp"
                     /locus_tag="PLJ64_02195"
     CDS             complement(7259..7738)
                     /gene="bcp"
                     /locus_tag="PLJ64_02195"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HNC92337.1"
                     /translation="MLEEGDKAPDFTLEDQLGEKVKLSDHRKELVVLYFYPKASTSGC
                     TTQACSIRDNSADYAKAGATVIGISPDEVKDISRFATKNDLEFTLVADKDHKVAEKYG
                     TWVEKSMYGNKYMGVQRATFIIRGGKILRVFPKVQPKKHDGLVLGALAEINEENRDV"
     gene            complement(7779..8663)
                     /locus_tag="PLJ64_02200"
     CDS             complement(7779..8663)
                     /locus_tag="PLJ64_02200"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HNC92338.1"
                     /translation="MADRGPLTLLGVPIDSVGTAGGTELGPNVLRQLLGPEGFEDAGD
                     TSRKIRGLERDRANGWLAFDDILAMTGEVRGRVADLTAAGKVPVLLGGCCTLLPGALA
                     GARDSLGEFGLAYFDGHLDLFTGLTSPTGEGADFPVAASLGMAPDPLLEVIGETPVVA
                     PGRMSLVGARDKEELQLISPLPEGLGIGRIEFRDDLRHADLNEVGASLEAELTDGGSG
                     FWLHLDVDVLDREAFPATDYLMPDGLSMDELRALFAPLASSPGLIGVDVTCFNPEKDP
                     DERCGTELAELLRGTLNG"
     gene            complement(8663..8995)
                     /locus_tag="PLJ64_02205"
     CDS             complement(8663..8995)
                     /locus_tag="PLJ64_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92339.1"
                     /translation="MDDSTSRYELEELVVQPGTYFNPQTEVVVVVDDSASIDQEIFNM
                     ESYEGADWVRIADDVAVDEQQRDELLEHFQTHYHPGNGGSVSGTALEQGDDELDEDEE
                     GQEIGRED"
     gene            complement(9025..9453)
                     /locus_tag="PLJ64_02210"
     CDS             complement(9025..9453)
                     /locus_tag="PLJ64_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92340.1"
                     /translation="MVAELSFVLILLCVVVVPVAAIAFARSGKAYEEIGKGRFAVDFD
                     DSSETDHHEELRQLVEAKAYRQARRGEQPVDVEREVERLSKGEPEETADAAPPPDPAT
                     AGIRAEIREVVIAKNESRERRGEEPLDVESEVERMLAEFG"
     gene            9594..10742
                     /locus_tag="PLJ64_02215"
     CDS             9594..10742
                     /locus_tag="PLJ64_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNC92341.1"
                     /translation="MVDFTLSDEQKDLREMAHNFAEKEMRSVAFEYDKDGTWPAEVIQ
                     KAWEVGLMNTHVDEAYGGPGLDYLSGCLIEEEFGWGCSGIGTSLACNGLASAPVALGG
                     SEETKAKYLGMLCEEPKLASFCLTEPDAGSDVAGMKTRAVKKGDKYVINGSKCFITNG
                     AHADWFTVYAKTDPDAGSRGITAFVVDKQDGITVDKKEDKMGQRASNTVTLTFDDVEV
                     PAENMLGEENKGFKLAMMTLDRTRPGVAAMATGIARSGFDAAVQYSKERQQFGVPIAM
                     HQAIQFLIADMAVKVDAARLLTWQSASQLDNGERNTLTSSEAKRFAADSAMEVTTDAV
                     QVFGGYGFIKEYHVEKFMRDAKIMQLYEGTSQIQRMVIAREVLLPRTV"
     gene            10859..11623
                     /locus_tag="PLJ64_02220"
     CDS             10859..11623
                     /locus_tag="PLJ64_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC92342.1"
                     /translation="MESTLAAPSQSQSSESPIVKAVGVERVYGEGQAEVRALDGVDVT
                     FEKGRFTSIMGPSGSGKSTLMHILAGLDKPTAGTVEIEGTDITGLDDGELTQLRRDKL
                     GFVFQFFNLLPVLTAEENILLPLSIARKKPDQEWVDQVIDRVGLSDRREHRPSELSGG
                     QQQRVAVARALVTKPAVLFADEPTGNLDSKASEDVLTMLRAAVDEVGQTVIMVTHEPD
                     AAAHGDRLIALRDGKLVHDAPPVSADNVIELLKTLA"
     gene            11648..14200
                     /locus_tag="PLJ64_02225"
     CDS             11648..14200
                     /locus_tag="PLJ64_02225"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC92343.1"
                     /translation="MLSLTFKNLWARKWRSLMTAVAVIFGIALVAGTYILTDTTNQAF
                     DQIFNESLAGTDVVVTAKSEVDQQDGSTPAFPAKILKKVDQVDGVKVAAGSIFTQGAI
                     LDDQGDSIGAGFSPQFIASTGPKVFEAVTYPEGHPPTNAHEATLDQAAADRKGIELGD
                     TIELVGTDKAVPFRVVGLTQLGGASFGGSSIAQVQLPVAQRVTGKVGEYDQISIAADS
                     GISSEELRDRVSEVVPKNLRVETAQENADRNASEIRDSLQFLTIALLAFAAIALFVGS
                     FVIFNVFSITVAQRTREFGMLRTLGASRGQILRTVVIEGLIIGLVGSLLGLVVGYGLA
                     AGLSAVLKAVGAELPANSLQVLPRTIIVSLLIGVGVTLVSSLIPAFRSTQVPPIAALS
                     ENLVVGGRNRVVVRTVIAVLLGVLGLALIAMTLISGKDGGSGAAQIGAGAVCVLIAIS
                     IFAPKLVSPAARIIGAPLERFGGLTGRLARENAQRNPSRTAVTAAALMIGLALIAFVT
                     IFASGLSSSVNKVIDDQLPGEITLQGQGGILPFPAGAIKVVQKVDGVEAASGVRYVSA
                     KVDGKTAAISSVDPADIVKVYTVEWEEGSDSDLTGLTDGQIVIGNKLADSLDVGVGDR
                     VTLTSQKGRKFPFQINAIMKTDSIALAGEGIIDQGAMERDFDQKSDAVGLVKLDPGAD
                     LDKTQTAIEDALTAQDFPTVDVLNQGELKEQQKSQIKGLLAMIYVLLALAVIVSLVGI
                     VVTLILSIYERTRELGMLRAVGMSRRQVKRMVRYEAVITAVIGAVSGLIVGVIFAFLI
                     GIPLGGDGFELSYPVGTLIIILILTALAGVIAAIYPARKAAKLDVLEAVSYE"
     gene            14237..16030
                     /locus_tag="PLJ64_02230"
     CDS             14237..16030
                     /locus_tag="PLJ64_02230"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HNC92344.1"
                     /translation="MSAFSRFLTLFMLIVLSCGALVTSNAGAANKNAYAMAMLKLHHP
                     AGLQKFARSVSSPNSDNYGQYLPVSRIVKKFGARSKAARRALRYFRSRGMRATLDGAH
                     QNVLVSMDLNQAGSYLAPSASASVARQGGPGSVPPALRGVASSLDFLDPDPEKYSTAG
                     KFPPQPSGSPFPEGSSARPHSGTQAGCAAGQRGATAIGEPNRNFTPNQFLDAYGISDL
                     NQQGFKGQGRRIAVIEIDGFKRSDIEAYGDCYGKRVPPTRVYPVYYKKPLAPGAETTL
                     DLEVITAVAPQLAGIDVYEGPGSEAGIMLMVGRALANQKKPAAISISLGACEPTLWRQ
                     LSYRRALGNLFAVAAGAGISVAVAAGDNGSAECSREGNSSTLPLLTVSDPASLEWVTA
                     VGGTNLELDANNGISQEFVWNDGSLGPSGGGGGFSILATRRPWYQQGTKRFRNYGLTR
                     AVPDVAALADSVPGYSVYCTAGVAGECANQYWPDGGWQPVGGTSAATPLTAALLTLVS
                     QKLGKSGRKPIGFANPLLYKLGKSQGASSVFRDVVAGNNDVGAAIPADQGGGKALGCC
                     YTKTGFDAASGWGSIKAPGLFKAALKLAARK"
     gene            complement(16036..17349)
                     /locus_tag="PLJ64_02235"
     CDS             complement(16036..17349)
                     /locus_tag="PLJ64_02235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC92345.1"
                     /translation="MVGGGLTGLWAAVRAKERNPAREVVLIEREQLATGASGRNGGFM
                     SSSLVHGLGNGLARFPDEVPLLEKLGLENLAGIEKVIEEQGIDCDLRKPGAIEVAVTD
                     AQVADLQGGVEELAGYGHEAVMLDGEEMRAEVNSPIYRGGLWQKSGEWSVDPVRLCDG
                     LAAYAERLGVRIHESTPMEGFEETGGGVEVRTANGSIKTERVLLATSAFRVPVRQIRH
                     RVIPVYDYVLVTEPLTRAQWVSLGWQNHQGMSDCSNQFHYYRPIDGGPAAATGQAAGG
                     RILWGGYDAIYNFGGKVETAAYQRDRSFAGLAQRFFTAFPQLEGVRFSHRWGGAIDTC
                     SRFFAFYGTTMGGKVGWAVGHTGLGVGASRFSAEVGLDLIDGVDNEITRLDYTRSKPM
                     PFPPEPLRYGVVQFTRSRIAAADRNDGRRGLWLRTLDRFGLGFDS"
     gene            17493..18245
                     /locus_tag="PLJ64_02240"
     CDS             17493..18245
                     /locus_tag="PLJ64_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNC92346.1"
                     /translation="MRRGRWADCSRPSRYLGTWFRASASGFQGHVRRNAKATTSGTSL
                     NFKPISEHELARLKDDELISHAVAARDARKQDAFEETLKLFVGRRFGLVEYWVSRKSN
                     ANDRDEIVSRAFVSIIKGIGKFRGRSTGQMVEWMRTVTGRRIADFYRDKEKTPRMVPL
                     GEQPKEDEDWGPTNLGNPDDTGGVEVEMLIEQTLETRNEVQQEVIRLRMKGYSSKEVA
                     EMSSDQKMSFTNVDKIFSRFRQDIEKAMQDDA"
     gene            18242..18910
                     /locus_tag="PLJ64_02245"
     CDS             18242..18910
                     /locus_tag="PLJ64_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92347.1"
                     /translation="MNDFEETPDPVIHAFDEYVAEFRKGHGDPSPFLERFEGSERKSL
                     ETLIESFVATGPESSPDPGDPRFNAVFEKVLDEVSAPAGGLSEHLALLRQKAGKTQAA
                     VVAALAKTFEATPQEQEKIDEYYHRLEWGSLPASGLDGKLYSALAAILGVEPESLKEA
                     AGSLGSRYGASSGPVFARSLDQVDLAEDASIVPGVIGQAVPDSESRSEPSRPDRIDEL
                     FTGG"
     gene            18933..19568
                     /locus_tag="PLJ64_02250"
     CDS             18933..19568
                     /locus_tag="PLJ64_02250"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HNC92348.1"
                     /translation="MPEEIRDPNRLVTTLEPAELARIEEEVQEIRACVPAWVWDGRSL
                     PVPIDLIAREIYNLRVRVVSEEAMREAIGEDPDGHYGLSGLLICADGEIWVSKGELDY
                     DWGSRRHRFTVGHELGHFVMHQSREPRIFCREIENGESETGEVDQNDPVPRPVPEAEA
                     NTFSAAMLMPIDQVRKRLSSNPADPVKDLQDYFGVNRKPALRRVETARILG"
     gene            complement(19582..21048)
                     /locus_tag="PLJ64_02255"
     CDS             complement(19582..21048)
                     /locus_tag="PLJ64_02255"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNC92349.1"
                     /translation="MAFAAAAFAAMLMLAAPASAQKLVTIDASSDYVDPSNQHLAGPV
                     TENMGPHPGKLMANVLLPDGYTPKKRYPLLLLLHGSGERFDSWADPLLGDIRNTAKGL
                     NAVIVMPEGAQGFYTDWWNEGERSGPAWEDYIRESLLPMIQSRFSIRPERRYHAIAGF
                     SMGGYGTWLTASQLPGFFGTAVPLSAFASIRTFESTLAFATAAGGTPYAQVYGDPTGF
                     YAEGHDPIALGPNLAFTNLDVFTGDGEPDPEVRPQNDPSNPGYVTDGDGVSLYLESVL
                     KTQNDAAVAAMEASGNKNVDYTVHKGSHDWEFWRPDLKAAIAKGLFRPTPARPANWSY
                     RTAASEGQAWDIYFNFSPAPTAVTTFKRTGNRLKVTGEGEMTIFDGNGCSFSADLPFT
                     RTLSEKPCRSLKLQAKGGGLRKGRTGTITVTVKGMNYTGVTKPVDAVKVEAGGASAIT
                     AYNGKATLKVKPKQSGKLTVKVAKDGFRPASAKLNVRK"
BASE COUNT         3638 a         6961 c         6874 g         3774 t
ORIGIN      
        1 cgggccggcc gcctccgccg ccatgaaacg ccttgccacc ggccagcagg tcacggtcac
       61 ctccgacccc acccaggatc gccgcgacca ttacggccgc ctcctcgcct acatcgacag
      121 gggcagccag gacctcggct acaccctcgt caaacgaggc ctcgcctccg cctatcccta
      181 cgacggtccc ttccagcgct acccgcgcta cgcccgagcc aacaaccacg cccgcaccaa
      241 caacatcggc agctggtccc actgcgacat ctcaccgggt tagggccggt ggccatcccg
      301 gtctgattcg gccagctcga ccaccttgtc gcgggccgat tcgccctgga tgatccggat
      361 ccttgttttc ggggtcttga actccttggc gaggagggcg atcagggctt ggttggcctt
      421 gccgtcaacc ggcggggcgg ttacccgggc ctgaaggacc tgctcgcctg aaccggggtc
      481 ctctttccag cccttcagct cgttcttcga ggccctgggc tgaaggcgga cgcggattcg
      541 ccggctgaat cctccggctc cggccgtcac ggttcagtag cggtggccgg aggtgtactt
      601 gcgggtggtg gtgccgaagt gctccacctt gccggaggcg accacatcca cgaactgctc
      661 gaggtgttcg gtcttccagc attcgaaggc gccgtcgcag aacggggtgt aggcgctcat
      721 caccgaatct ccgtagttcc agtagagctc cggttccggg ttgagccaga aggtgcggtt
      781 ggcgcggtcg gcgacccggg cgaaggcgtc ctcggccggc ggacggccgt tggtcctggc
      841 gtcgccgagc acgatcaccg tcgagcgggg cccgaggtcg gccgaaacat cggcgtagaa
      901 ctcgttccac acccgcccgt aatcggtgta accggaaatg tcagcgaccc cgccggtcgc
      961 ggtgatcttc tcggcgatcg cggtgaaatc ccgttcgtcc tcgaatacgt gcgtcacctc
     1021 cgagatccgt tcgatgaaga cgaagctgcg cagcttgcgg aacgaatcgt ggagcgcgtg
     1081 aaggaccgaa agaaagaagg tcgaggccga gctgaccgaa gtcgagacgt cgcagagcac
     1141 gtagatctcc ggcttgcgcg gccggcgcgg gcggtacttc agattgagcg gaaccccgcc
     1201 ggtctcaagc gaggagcgca tcgttcgccg catgtcaacc gtccggccgc gcttcgcccc
     1261 gcgcgggtcg tggccgagcg tggccaggcg gcgcttcatc tgggcgaccg ccttgtgcac
     1321 ggccgcgagg tcctgggcgc cgcgcatcgg cagggcccgg ttcaggtccg agagcggccg
     1381 ggccgggggc agctggccct gcttctggat ctgctggcgc tggagttcgc gccggagctg
     1441 gcgttcgaag cgtgcgatct ggtcccggtc aaggttctcg ccatcggctt cgccgtcacc
     1501 gcttgtgtcg gatgaggggg agaggccgag cccgcggcgg atccgctgca tgtcgacccc
     1561 gaccaccccg gagctttcac cctggcggcc gaaggcctcg atcgccagcc gggccagttc
     1621 gttcatcgcc gattcgtcgc cctcggcgat cgcctggcga atcgcctcgg ccagggcctc
     1681 catgctcagt tcctcgcgga cctcgtcgga gagcgcggcc ttgcggccct cgccaccctc
     1741 cggcgcgaac tcgagcgagg cggcttcagt cgcccggaag aaatgccggt cgaagacgag
     1801 gtcgaaaatc tcgcggtcct tcggcgactt ggccagcgtg gtggccagcg attcccggaa
     1861 gtcgaccggc tcggtccagg gcacgatctc cagcgcccgg aaggcgtcct ggatttccga
     1921 ggtgccgatc gcgacatcct ccgaacgaag ctcctcgcag aaggcgagga tctgctccgc
     1981 cattcccgaa ggggtctcgc tcatcggatg gctcccgtcc tagccggcgc cgttggcgga
     2041 gctgccgttg gacgatccgg aaccgccgag ccgtggcacg atcttctcgg cgaccagatc
     2101 gaggtcggtg cggtgtttga tgatgatcga aagcgactcg cgaaagacct tctcgtcgat
     2161 gtcgtcggcg cccatcagga tcagggtccg ggcccagtcg atcgcctcgg caatcgaagg
     2221 cggcttcttc agatccagct cgcgcaccat gtcgaccacg tcaaccagcc ggccggccag
     2281 ggcctcgtcg atttccggcg cgtgaagccg gatgatctcg atctcccgat cccgggccgg
     2341 ataatcgagc cagaggtaga ggcagcgccg tttcagggcc tcggtcagct cgcgcgaatt
     2401 gttcgaagtc agcagcacga tcgggtgggt cgtggcgctg atctgcccca tctccggaat
     2461 cgtgatctgg aaatcggaaa gcagctcgag cagcatcgcc tcgaactcct ggtcggtctt
     2521 gtcgatctcg tcgatcagca ggaccaccgg gtccgggttg gcgatcgcct tcatcagcgg
     2581 ccgctcgagc aggaaatcct cggtgaagat ctcgcccatg ccgaccggag tcgcgccttc
     2641 gtcctgggtc gatgcggtgg cctgaatctg cagcagctgc ttgcggtagt tccactcgta
     2701 gagggccttc gcctcgtcga gcccctcgta gcactggagc cggaccagct cccggccggt
     2761 tgcccgggaa agggccttgg ccagttcggt cttgccgacc ccggccgggc cttcgaccag
     2821 aaccggcttg cccagccgct gggcgaggaa agcgatcagc gcagttgaat catcggccag
     2881 atacgacgca tcgcgaagag cttcggcgac ttcggcggga ctccggggaa cggcttgagg
     2941 ggtggctgcc atgccttgga caatacgccg accgcgccgg aacccgggtt catgccggtt
     3001 tcagtcgagg ggaatgtcag tgacccgcgg ggtcgccggc tcttcgggct gatccgaagg
     3061 tgacggctcc gggccggagg gaccgcccgg tgaggagttg ccgctcttgt cggcgtatgc
     3121 gtcgaggaat gccttcagca cgccgatcgc ctcatgctgg atcgattccg cctgctggcg
     3181 caactcgggt gagacggtgt ggcgaaaaag ctcggccgaa cggcagatcg ggcaccactc
     3241 gaggcattcg gcgccgtgcc cctgaccatc cggaccttcg cttcccgctt tcgccgattc
     3301 tgctcccgag ccgttttcgg ccgaactccg gccggctttt gactgtgaga actggcccca
     3361 gaagcggtcg gcaccgggtt gctgttcggt ttgttcaggc ttgggtggca tgcggatcct
     3421 cgaagatgat ctccagggtc tcgtcctcga atgaggcttt cgacgggctg cgtctggcaa
     3481 gcccggacgg gagcatgata gtccgccttg cctcgccgac ctgaaccatc agctcttcgc
     3541 cggcctgctg gagtttcact tcgcccttct cggtgaatgg cgcaaggatg cgaattacgg
     3601 tccggccatc ctcggttgcg acctcgcgcg gcatgccgga gtggagcagg ccggaaggat
     3661 cgatctcccc gtaaagatcg tcggcgagcc gctcatgcat ctcttcgccg atcacttccc
     3721 ggtcgaagta gcgggcggta agtaccggca ccggggagaa gccttcctcg accgattcga
     3781 gctgcgtggc ctgctgctcg cgccagccgg cgaaatagga atcggcgact tcgtcaggga
     3841 agacgcggtt gacgatcacg gcgtcggtca ggtacccgaa caggcagagg tgggtgaagg
     3901 tgcgccgcgc ttcgttgatc accatccggt ccggattcat gaccagccgg atcgagcaat
     3961 gctcgtggtc acggaggatc ccgtccatcg ccagcaggtt ctcgatcagg tgctcgacct
     4021 cgtccatcag ctcgggctcg ggcatcggga tctcgagcgc cttggcgatc ggcgccgcct
     4081 tgctcagcag gctgcggttc caggggaaga cctttcgcac ccaccagctt gcgacttccg
     4141 ggtagccgag caaccgcagg gtttcgccgc tcggagcgca atcgacgatc accacgtcaa
     4201 attccgactt gtcccagtgg cgcttgatct gaagcagcga gaaaagctcg tcgagtccgg
     4261 gcgggacgct caactcctcg gcccgcacct ggtcgacccc cttgctctgc agcagcccgc
     4321 tcatccaggc ggcgacccgg tcccagttgc ggcgcatctc ggcttcggcg agcacctcct
     4381 ggccccaaag cccgggcgaa acctcgaccg gatcagggcc gagttcgacc ccgagggaat
     4441 cggagaggct gtgggcgggg tcggtcgaga ggatcaccgt gcgtctgcct tcggctgcgc
     4501 agcgacgggc ggtggcggcg gcaacgctgg tcttgccaac gcctcctttg cctgtgtaga
     4561 ggatggtgcg gggtggcatt caagggcgag cgtatagcct gccccgactt ggaacggacg
     4621 cggatccccc ttgttctggt gctcgtgctg gcgttctcgc tggtgatgca ggcctgcggc
     4681 agcagtgacc cggacccggc gacgattctg gaccgggccc tgaccaggga gcatctttcg
     4741 agcttcggca acggatcggg cggtcccacc gggggcatcg tttcggtcga ggcacttggc
     4801 tacgaggacc gggttctgga cgagcggaag gtggcggccg acccgcaggt gatgtctgac
     4861 atccgcagcg ccctgggctc cgattcgggg ctgcgcggac tggtcggcga ccttcggtac
     4921 gacgggaccg aagaggtttc cggggtcggg accgaccaca tctccggtga actcgacgtg
     4981 gccggcctgg ccagggcact ggaagcggcc gggggcgggg aggtgggcca gatcgccggg
     5041 gtcaaaaccg gatccagcct tgaggattca ctcgtttcag ccgatttcga cctgtacgcc
     5101 ggccagcagg atggcgacat ccgccggctc gatctgaccc tggcccttga cgacccggac
     5161 aatgcacttc cgcctgcccg gatacggttc agcctgacgc cgcgaccatc cggcgcaaac
     5221 cagacctaga ggacctcccc cttacccatg gaatacctgc tcctgctttt cagcttcgcg
     5281 atcatcctga ccggcgcgct gctcttcacc aacgcgatcg agtggctcgg tcacaagctc
     5341 aacctgggtg agggcgcggt cggttcgatc ctggcggcag tgggcaccgc gatgccggaa
     5401 accctgatcc cgatcgtcgc gatcatcggc ggggtcagtg gctccgacga ggttgcggtc
     5461 ggggcgatcg tcggggcgcc gttcctgctg gcgacgatcg cgatggccct ggtcggcatc
     5521 tcggcgctcg cctacatcaa acgccggccg cagggcaagc acctcgacgt ccacgtcgaa
     5581 accctcgacc gggacctggt cttcttcatg atcttcttca ccctggccct ggtggtcggg
     5641 gtggttgacg ttccacagtc ggtgcagatc ggcggcggag tgatctgcct cctggcttac
     5701 gtctactacg tggtgcagac gatccgcaac agcggtgacg ttgccgagag ccacgagatg
     5761 gatcccctga tcatggatcg ccggaccgac cgcaccggcg ggccgcccaa ctcgatcatg
     5821 atcatccagc tgctggtcgg tctcgcctca atggtcggcg gtgcccatct cttcgtccac
     5881 gaactgctga gcgtggccga gcatctcggc gtttcggcga tcatcctctc gctgatcctc
     5941 gcgccgctgg cgaccgagct gccggagaag gcgaactcgt tcttctgggt ccgcgacggc
     6001 aaggatgccc tcgccctcgg caacatcacc ggagcaatgg tctttcagtc gaccatcccc
     6061 gtcgcgatcg gcatgatctt catcgactgg tcgctggaca agtacgcggt cctctcgatg
     6121 ctcctcggcc tcgccggcgg tgtcgtcgcc tactggtcac tccgccggaa cggccagttc
     6181 aacaagcctt cgatcgcgat ctggggtctg ctctacctga ccttcctcgc cttcgtgatc
     6241 ctcagcggcg acaccggcca ctcgaccgcg cattagccgg aaaaacccag gcgagacgca
     6301 acgtcccgtc tcgccgcagg accacaaaac gtcaaatcca gacgagacgc aacgtcccgt
     6361 ctcgccgaag gaccacaaaa cgtcaaatcc agacgagacg caacgtctcg tctcgccgca
     6421 ggttcacaaa acgtcacatc caggcgagac gcaacgtctc gtctcgcggg agttttttca
     6481 gacggggctg ccctcgaaga ggaggtaacg cagcgtgttg cggaagcttc cgtcgctggc
     6541 ttcgttgtgg gattcgacca ccggcaacgc ctcgcggcgc atctcttcgt aggcctcgtc
     6601 gccgatcact tcctgcatgg tgatccagat cgggtgaagg tcgaaccagc ggtcgttcag
     6661 cgctgcggcc gattcctctt cgatcacgag ttccctggtt tcgacctgca gcatgaggcc
     6721 gctctcggag aacagttcgc gtacggcggc ctcgtcgccc caggcgaacg gagcgtcggc
     6781 cccttccggc ggtgcctggc caaagtggga agcaactgcc gccttagcca tgtcctgcat
     6841 gtactggaag aggccttcct cgacccacga agtgaaggcg atccggccct ggggccggag
     6901 gacccggccg atctcggcgg cggaatcctg cggcgagccg gtgaagatga gcccgaagac
     6961 agaggtgacg atctcgaagc tgtcgtcgtc gaagggaagc tccgcgaggt cgccctgaac
     7021 ccgttccagc tcgagtccct cggctgcggc tctctcgccc gccacctgga gcaggcgcgg
     7081 ggcaccgtca tatccggtca ccctggcgcc gcggcgggcc agctcgagcg cttcgtttcc
     7141 ggtgccgcag gcgaggtcga gggcgagctt gccggtgacc ggttcgacgg catcggcgac
     7201 cgcgggagtg gcgggcgcga tctgttcggc agtgacctcg tatgagccct tgtcccagtt
     7261 agacatctcg gttctcctcg ttgatctcgg ccagtgcccc caggacgagg ccatcgtgct
     7321 tcttcggctg gaccttgggg aagacccgca ggatctttcc gccccggatg atgaaggttg
     7381 cccgctgcac gcccatgtac ttgttcccgt acatcgactt ttcgacccag gtgccatatt
     7441 tctccgcgac cttgtggtcc ttgtcggcga ccaaggtgaa ctcgaggtcg ttcttggtcg
     7501 cgaaccttga gatgtccttg acctcgtccg gcgagatccc gatcaccgtc gcgcccgcct
     7561 tcgcgtaatc ggcactgttg tcgcggatcg agcaggcctg ggtggtacac ccgctggtac
     7621 tggccttcgg atagaagtag aggacgacca gttccttgcg gtgatcggag agctttacct
     7681 tctccccgag ctggtcctca agtgtgaaat cgggagcttt gtcgccttct tcgagcatgg
     7741 cgggatctta tggggcactc atggcgcgag ccgccgggtc atccattcag ggttccgcgc
     7801 agcagttcgg ccagctccgt cccgcatctc tcgtcgggat ccttttccgg gttgaagcag
     7861 gtgacgtcaa ccccgatcag gcccggagag gaagccaggg gcgcgaacag ggcgcggagc
     7921 tcatccatgc tcaacccgtc gggcatcagg tagtccgtcg cggggaaggc ttcacggtcg
     7981 aggacgtcga cgtcgaggtg aagccagaat ccggagccac catcagtcag ttcggcctcg
     8041 agcgaggcgc cgacttcgtt caggtcggcg tgcctgagat cgtcgcggaa ctcgatccgg
     8101 ccgatgccga gaccctccgg cagcggcgag atcaactgga gctcctcctt gtctcttgcg
     8161 ccgaccagag acatccgccc gggcgcgacc accggtgtct cgccaatcac ctcgagcagg
     8221 ggatcgggag ccattccgag cgatgcggcg accggaaagt cggccccttc accggtcggc
     8281 gaagtcagcc cggtgaaaag gtcgagatgg ccgtcaaagt aggcgagacc gaattcgccg
     8341 agcgaatccc gggctccggc cagcgcgccc ggcaaaaggg tgcagcagcc gccgaggagc
     8401 accggcacct tcccggcggc ggtcaaatcc gcgacccggc ccctcacctc gccggtcatc
     8461 gccaggatgt cgtcgaaagc cagccagccg ttggccctgt cccgctccag tccgcggatc
     8521 ttgcggctgg tgtcgccggc gtcctcgaac ccttcgggcc ccagcagttg ccgcaagacg
     8581 ttcggtccga gctcggtacc tccggccgtg cccacgctgt cgatcgggac gcccaggagg
     8641 gtcagcggcc cgcgatcggc catcagtctt cgcggccgat ttcctggcct tcttcgtcct
     8701 cgtcgagctc gtcgtcaccc tgttccaggg cggtgccgga aaccgacccg ccgtttccgg
     8761 ggtggtagtg agtctggaaa tgctcgagca gttcatcgcg ttgctgctcg tcgaccgcga
     8821 catcgtcggc gatcctcacc cagtcggcgc cctcgtagga ctccatgttg aagatctcct
     8881 ggtcgatcga cgctgaatcg tcaaccacga caacgacttc ggtctgcggg ttgaagtagg
     8941 tccccggttg aaccaccagc tcttcaagtt cgtaacggga agtcgagtca tccatgagcc
     9001 aatactaccc acgacccgtc gccgctagcc gaactcggcc agcatccgct cgacttcgga
     9061 ttcgacgtca agcggttcct cgccccggcg ctcgcggctc tcgtttttcg caatgaccac
     9121 ctcgcggatt tccgcgcgaa ttcctgcggt cgcgggatcc ggcggcggag cggcatcggc
     9181 cgtttcttcc ggttctcctt tcgagagccg ctcgacttcc cgctcgacgt caaccggttg
     9241 ctcgccccgt ctcgcctggc ggtaggcctt ggcctcgacc agctgtcgga gctcctcgtg
     9301 atggtcggtc tcgctgctgt catcgaagtc gactgcgaat cgtcccttcc cgatttcttc
     9361 gtacgccttc cccgagcggg cgaaggcgat cgcggcgacc ggcaccacga ccacgcaaag
     9421 gaggatcagg acgaaggaaa gttccgcaac catcccccca agagtaaagg cgcttggtac
     9481 cctgttcgaa gagttattga ttgacaagtc aatcgggagc cgcccgggcc tcctcgcccc
     9541 ggccccgcat cgagcaaatc gaacggttcc aaccgaccta aggagcttga agagtggttg
     9601 atttcacgct gagcgacgag cagaaggatc tgcgcgaaat ggcgcataac ttcgccgaga
     9661 aggagatgcg aagcgtcgcc ttcgaatacg acaaggacgg cacctggcct gccgaagtga
     9721 ttcagaaggc ctgggaagtc ggcctcatga acacccatgt agacgaggct tacggcggcc
     9781 cgggactgga ttacctttcc ggctgcctga tcgaggaaga gttcggctgg ggctgctccg
     9841 gcatcggcac ctcgctcgcc tgcaacggcc tcgcttcggc cccggtcgcc ctcggcggct
     9901 ccgaagagac caaggccaag tacctcggca tgctctgtga ggagccgaag ctcgcctcct
     9961 tctgcctgac cgagccggac gccggctccg atgtcgccgg catgaagacc cgcgccgtga
    10021 agaagggtga caagtacgtc atcaacggtt ccaagtgctt catcaccaac ggtgcccacg
    10081 ccgactggtt cacggtctac gccaagaccg atccggacgc cggcagccgc ggcatcaccg
    10141 ccttcgtggt cgacaagcag gacggcatca ccgtcgacaa gaaggaagac aagatgggtc
    10201 agcgggcctc gaacaccgtt accctgacct tcgacgacgt cgaggttccg gccgagaaca
    10261 tgctcggtga ggagaacaag ggcttcaagc tcgccatgat gaccctcgac cgcacccgtc
    10321 ccggtgtggc cgccatggca accggcatcg cccgctccgg cttcgacgcc gccgtccagt
    10381 actcgaagga gcgccagcag ttcggcgtgc cgatcgcgat gcaccaggcg atccagttcc
    10441 tgatcgccga catggcggtc aaggtcgacg ccgcccgcct gctgacctgg cagtccgcca
    10501 gccagctcga caacggcgag cgcaacacgc tcacttcatc cgaggccaag cgcttcgccg
    10561 ccgactcggc gatggaagtc accaccgacg ccgtccaggt cttcggcggc tacggcttca
    10621 tcaaggagta ccacgtcgag aagttcatgc gtgacgccaa gatcatgcag ctctacgaag
    10681 gcaccagcca gattcagcgc atggtgatcg cgagagaagt actgctccct cgcaccgtgt
    10741 agccaaggcg acacctgtta cctcgaaagg gcccggcaaa cgccgggccc tttctctttg
    10801 gaggctttgg ggggctaacc gaacgtccgg aaatttcgcg ggtgttaggt tggcggggat
    10861 ggaatccact cttgccgctc ccagtcagtc ccagtcctcc gagagtccga tcgtcaaggc
    10921 cgtcggcgtc gaacgtgtct acggcgaagg ccaggcggag gtgcgtgccc tcgacggggt
    10981 ggacgtgacc ttcgaaaagg gccgtttcac ttccatcatg ggcccttccg gttccggcaa
    11041 gtcgaccctg atgcacatcc tcgccggcct cgacaagccc accgcgggca cggtcgagat
    11101 cgaagggacc gacatcaccg ggcttgatga cggcgagctg acccagctcc ggcgcgacaa
    11161 gctcggcttc gtcttccagt tcttcaacct gctgccggtg ctgacggccg aggagaacat
    11221 cctgctgccg ctctcgatcg cccgcaagaa gcccgaccag gagtgggtcg accaggtgat
    11281 cgaccgggtt ggtctgagtg accggcgcga acaccggcct tcggagcttt ccggcggcca
    11341 gcagcagcgg gtcgcggtgg cccgggccct ggtcaccaag ccggccgtgc tcttcgccga
    11401 cgagccgacc ggaaacctcg actcgaaggc atccgaggac gtgctgacga tgcttcgggc
    11461 cgcggttgac gaggtggggc agacggtgat catggtcacc cacgaacccg acgcggccgc
    11521 ccacggcgac cggctgatcg ccctgcgcga cggcaaactg gtgcatgacg cgccgccggt
    11581 ttcggcggac aacgtgatcg agctgctgaa gaccctcgcc taggcccgtc cggaccggag
    11641 ccgcaccatg ctcagcctga ccttcaagaa cctctgggcc cgcaagtggc ggtcgcttat
    11701 gaccgcggtc gcggtgatct tcggcatcgc cctggtcgcc ggcacctaca tcctgaccga
    11761 caccaccaac caggcgttcg accagatctt caacgaatcg ctggccggca cggacgtggt
    11821 ggtgacggcg aaatccgagg ttgaccagca ggacggcagc acccctgcct tcccggcgaa
    11881 gatcctgaag aaggtcgacc aggtcgacgg cgtgaaggtg gccgccggtt cgatcttcac
    11941 ccagggcgcg attctcgacg accagggcga ttcaatcggc gcgggtttct ctccccagtt
    12001 cattgcctcg accggcccaa aggtattcga ggctgtcacc tatcccgaag gtcacccgcc
    12061 gaccaatgcc cacgaggcga ccctcgatca agccgccgcc gaccggaagg gaatcgagct
    12121 tggcgacacg atcgagctgg tcggcaccga caaggcggtt cccttcaggg tcgtcggtct
    12181 cacccagctt ggcggtgcct ccttcggcgg ttcctcgatt gcccaggttc agttgccggt
    12241 cgcccagcga gtgaccggaa aggtcggcga atacgaccag atctcgatcg ccgccgacag
    12301 cggtatctcg tcggaggaac tgcgcgaccg ggtctccgag gtcgtgccga agaacctgcg
    12361 ggttgagacc gcgcaggaaa acgccgatcg gaacgcaagc gagatccgtg acagcctcca
    12421 gttcctgacc atcgcgcttc tcgccttcgc ggcaatcgcg ctgttcgtcg gttcgttcgt
    12481 gatcttcaac gtcttctcga tcacggtcgc ccagcggacc cgtgagttcg gaatgctccg
    12541 taccctgggg gccagccgtg gccagatcct gagaacggta gtgatcgaag gtctgatcat
    12601 cggcctggtc ggatccctgc tcgggctcgt ggttggttac ggcctggccg caggcctgag
    12661 cgcagtgctc aaggcggtgg gggcggagct tcccgcgaac tcgcttcagg ttctgccccg
    12721 cacgatcatc gtgtcgctcc tgatcggggt cggcgtgacc ctggtctcat cgctgatccc
    12781 ggccttccgc tcgacccagg tgccaccgat cgcggcgctc tcggaaaacc tggtggttgg
    12841 aggcaggaac cgggtcgtcg tcagaacggt gatcgctgtt ctgctcgggg tgctcggcct
    12901 ggccctgatc gcaatgaccc tgatcagtgg caaggacggc ggcagcggcg ccgcccagat
    12961 cggagccgga gcggtctgcg tgctgatcgc gatctcgatc ttcgccccca agctggtttc
    13021 gcccgcggcc cggatcatcg gtgcgccgct ggagcggttc ggcggcctca ccggccgcct
    13081 cgcccgcgag aacgcccagc gaaacccgtc acgcaccgcg gtcaccgccg cggcgctgat
    13141 gatcgggctc gccctgatcg ccttcgtcac gatctttgct tccggcctca gttcgtccgt
    13201 gaacaaggtg atcgacgacc aacttccggg cgagatcacc ctgcagggtc agggcggcat
    13261 cctgccgttt cccgccggcg cgatcaaggt tgtccagaag gtcgacgggg tggaagccgc
    13321 ttccggcgtc cgttacgtat cggcgaaggt cgatggcaag acggccgcga tctcctcggt
    13381 cgatccggcc gacatcgtca aggtctacac ggtcgagtgg gaggaaggtt ctgattcgga
    13441 tctgaccggt ctgaccgacg gccagatcgt gatcggcaac aagctcgccg actcgctgga
    13501 tgtcggggtg ggagacagag tcaccctgac ctcacagaag ggccggaaat tccccttcca
    13561 gatcaacgcg atcatgaaga ccgacagcat cgcgctggcc ggcgagggca tcatcgacca
    13621 gggcgcgatg gagcgggact tcgaccagaa gtccgatgcg gtcggtctgg tcaaactcga
    13681 ccccggtgcc gatctcgaca agacccagac cgcgatcgag gacgcgttga cggctcagga
    13741 cttcccgacc gtcgacgtgc tcaaccaggg cgaactgaag gaacagcaga agtcccagat
    13801 caagggactt ctggcgatga tctacgtgct gctcgcgctg gccgtgatcg tttcgctggt
    13861 cggcatcgtg gtcacgctga tcctctcgat ctacgagcga acccgcgaac tcggcatgct
    13921 cagggcggtc gggatgtcac gccgtcaggt caagcggatg gttcgctacg aagcggtgat
    13981 cacggccgtg atcggggccg tctccggcct gatcgtcggc gtcatcttcg ccttcctgat
    14041 cggcatcccg ctcggcggtg acggcttcga actgagctac ccggtcggca ccctgatcat
    14101 catcctgatt ctcacggcgc tggccggagt gatcgccgcg atctaccccg cccgcaaggc
    14161 ggccaagctc gacgtgctgg aagccgtttc gtacgaatag cgtttgacct gcggtccagg
    14221 cgggaacttg ccggccatgt cggctttttc gcgcttcctc actttgttca tgctgatcgt
    14281 tctttcatgt ggggcgctgg tcacatcgaa tgccggtgcc gccaacaaga acgcctacgc
    14341 gatggcgatg ctcaagcttc accatcccgc cgggttgcag aagttcgcga ggtcggtcag
    14401 cagtccgaat agcgacaact acgggcagta cctaccggtg agcaggatcg tcaagaagtt
    14461 cggcgcccga tcgaaggccg ccaggcgggc cttgcgttac ttccgttccc ggggcatgcg
    14521 ggccacgctc gacggtgcgc atcagaacgt actggtgagc atggacctga accaggccgg
    14581 aagctacctc gctccgtccg cctcggcctc ggttgcccgt cagggtggcc cggggtcggt
    14641 gcctcccgcc ctgcggggcg tggcaagcag ccttgacttc ctcgacccgg atccggagaa
    14701 gtactcgact gcgggcaaat tcccgcctca gccgtcgggc agtcccttcc ccgagggcag
    14761 ctcggcccga ccccattccg gaacccaggc gggctgcgcg gccggtcagc gcggagccac
    14821 cgcgatcggt gaaccgaacc gcaacttcac ccccaaccag ttcctcgacg cctacggcat
    14881 cagtgacctc aaccagcagg gattcaaggg tcagggccgg cggattgcgg tgatcgagat
    14941 cgacggcttc aagcggtccg acatcgaggc ctacggcgac tgctacggca agcgggtccc
    15001 gccgaccagg gtctacccgg tctactacaa gaagccgctg gcgcccggcg ccgaaaccac
    15061 cctcgatctc gaagtgatca cggcggtcgc tccgcagctt gccgggatcg acgtttacga
    15121 agggcccggc agcgaggccg ggatcatgct gatggtcggc cgcgccctcg caaaccagaa
    15181 gaagccggcg gcgatctcga tctcgctggg cgcctgcgaa ccgaccctct ggcgacagct
    15241 ttcataccgc cgcgcgctag gcaacctgtt cgcggtcgcg gccggtgccg gcatctcggt
    15301 ggcggtggcg gccggggaca acggctccgc cgagtgctcg cgcgagggga attcatcgac
    15361 cctgccgctg ctcacggtca gcgatccggc cagcctcgag tgggtgaccg ccgttggcgg
    15421 aacgaacctc gaacttgacg ccaacaacgg catctcgcag gagttcgtct ggaacgacgg
    15481 ttcactcggt ccgtccggcg gcgggggcgg cttcagcatc ctcgccaccc ggcgcccctg
    15541 gtaccagcag gggaccaagc ggttcaggaa ttacggtctg acccgcgccg tgcccgacgt
    15601 cgctgccctc gctgacagcg ttcccggcta cagcgtctac tgcacagccg gggtcgccgg
    15661 tgaatgcgcg aatcagtact ggccggacgg cggctggcag ccggtcggcg gcacctcggc
    15721 agcgaccccg ctgaccgccg cgctcctgac cctggtttcc cagaaactcg ggaagtccgg
    15781 ccgcaagccg atcgggttcg cgaatccgct cctctacaag ctcggcaaga gccagggggc
    15841 atcctccgtg ttccgcgacg tcgttgccgg caacaacgac gtgggcgccg cgatcccggc
    15901 cgatcagggt ggcggcaagg cgctcggctg ctgctacacc aagaccggat tcgacgcggc
    15961 aagcggctgg gggtcgatca aggctcccgg gctcttcaag gcagccctga agctggccgc
    16021 gagaaagtag tcgactcagg aatcgaagcc gaggccgaag cggtcgaggg tgcggagcca
    16081 gagtccgcgg cgaccgtcgt tgcggtcggc ggcggcgatg cggctgcggg tgaactggac
    16141 gacgccgtag cgaagcggtt cgggcgggaa cggcatcggt ttcgaccgcg tgtagtcgag
    16201 ccgggtgatc tcgttgtcga ccccgtcgat caggtcgaga ccgacctcgg ccgagaaccg
    16261 gctggctccg accccgaggc cggtgtggcc gaccgcccag ccgaccttgc cgcccatcgt
    16321 ggtgccgtag aaggcaaaaa agcgcgagca ggtgtcgatc gcgccgcccc agcgatggga
    16381 gaaccggacc ccttccagtt gcgggaaggc ggtgaagaaa cgctgggcga ggccggcgaa
    16441 gctgcggtcc cgctggtagg cggcagtttc caccttgccg ccgaagttgt agatcgcgtc
    16501 gtagccgccc cagaggatcc ggcccccggc cgcctggccg gtcgcggccg ccggcccgcc
    16561 gtcgatcggc cggtagtagt ggaactggtt cgagcagtcc gacattccct gatggttctg
    16621 ccagccgagc gagacccact gggcgcgggt cagtggctcg gttaccagca catagtcgta
    16681 gaccgggatc acccggtggc gaatctgccg gaccggcacc ctgaaggccg aggtcgcgag
    16741 cagcaccctt tcggtcttga tcgagccgtt cgcggtgcgg acttcgactc cgcccccggt
    16801 ttcctcgaag ccttccatcg gggtcgactc gtgaattcgc accccgagcc gttcggcata
    16861 ggcggccagg ccgtcgcaaa gccggaccgg atcgaccgac cactcgcctg acttctgcca
    16921 gaggccgccg cggtagatcg gcgagttcac ttcggccctc atctcctcgc cgtcgagcat
    16981 cactgcctcg tgcccgtacc cggccagctc ttcgaccccg ccttgaaggt cggccacctg
    17041 ggcgtcggtc accgcgacct cgatcgcccc gggcttcctc aggtcacagt cgatcccctg
    17101 ctcctcgatt accttctcga tcccggccag attctccagg cccagcttct cgagcagcgg
    17161 cacctcatcc gggaagcggg cgaggccatt gccgaggcca tggaccagcg aggaagacat
    17221 gaagccgccg ttgcgtccgc tcgccccggt cgcaagctgt tcacgctcga tcagcacgac
    17281 ctcccgggcc gggttgcgtt ccttcgcccg gaccgccgcc cagagcccgg tcagcccgcc
    17341 cccgaccacg accagatccg cctcggagga accttcgaga gggtggtgcg gttcgggtcg
    17401 cggcccggaa agccagaacg gcgaccgctc ggcttcggca aaggagcgac gatcggattc
    17461 acctggttcc gattcgggcc agactgcgtt ctgtgcgccg cgggagatgg gctgactgta
    17521 gccgacccag ccggtatctt ggcacttggt tccgggcgtc cgcgagcgga tttcagggcc
    17581 atgtcagaag gaatgcgaag gcaacgacct ccgggacaag cttgaacttc aagccgatca
    17641 gtgaacatga actggcgaga ctgaaagatg acgaactcat ctcccacgct gttgctgccc
    17701 gggatgcgcg caaacaggac gctttcgagg agaccctcaa actctttgtc gggcgccgct
    17761 tcgggcttgt cgagtactgg gtcagcagga agtccaatgc caacgaccgg gacgagatcg
    17821 tcagccgggc gttcgtcagc atcatcaagg gcattggcaa gtttcggggc cggagtaccg
    17881 gccagatggt cgagtggatg cgaaccgtca ccggacggcg gatcgccgac ttctacaggg
    17941 acaaggaaaa gacccccagg atggtcccgc tgggcgaaca gccgaaggaa gatgaggact
    18001 gggggccgac gaacctcggc aaccccgatg acaccggcgg cgtcgaggtc gaaatgctga
    18061 ttgagcagac tcttgaaacc cggaacgagg tccagcagga agtgatccga ttgcggatga
    18121 aagggtattc ctcgaaagag gttgccgaaa tgagcagcga ccagaaaatg tctttcacca
    18181 acgtcgacaa gatcttctcc cgttttcggc aggacatcga aaaggcaatg caggacgacg
    18241 catgaacgat ttcgaggaaa ccccggaccc ggtcatccac gcattcgacg agtacgtggc
    18301 cgagttccgc aagggccacg gcgatccgtc gcctttcctc gagcgtttcg aagggagcga
    18361 acggaagtcc ctcgaaactc tgatcgagtc cttcgtggca accgggcccg agtcaagccc
    18421 ggacccgggt gacccccgat tcaacgcggt cttcgagaag gtcctcgacg aggtttcggc
    18481 tccggcaggc ggtctttccg aacatctcgc cctgctgaga cagaaggcag gcaagaccca
    18541 ggctgcggtg gttgccgctt tggcaaaaac ctttgaggcc accccacaag agcaggaaaa
    18601 gatcgacgag tactaccacc ggctcgaatg gggttcgctg ccggccagcg gcttggacgg
    18661 aaaactctat tcggcactgg ccgcgattct cggcgttgaa ccggagtcat tgaaggaggc
    18721 ggccggttcg cttggttcca ggtatggcgc ttcatcagga ccggtttttg cccgttccct
    18781 ggatcaggtc gatctggccg aggatgcttc gatcgttccc ggcgtaatcg gtcaggccgt
    18841 gccggattct gaatcccggt cggaaccgtc cagaccagat cgaatcgacg agcttttcac
    18901 tggcggctga cccgccttct acaatcgcct gcgtgccgga ggagatcaga gacccgaaca
    18961 ggctcgtaac cacgctcgag cctgccgagc ttgcccggat agaggaagag gttcaggaaa
    19021 tcagggcctg cgttcccgca tgggtctggg atggccgcag cctgccggtg ccaatcgacc
    19081 tgatcgcccg cgagatctac aacctccggg tgcgcgtggt cagtgaggaa gcgatgcgcg
    19141 aagcgatcgg tgaggatccc gacggtcact acgggctatc cggcctgttg atttgcgctg
    19201 acggtgaaat ctgggtcagc aagggcgaac tcgattacga ctggggttcg cgacggcacc
    19261 gattcacggt tggccacgaa ctcggccact tcgtgatgca ccagagccgg gagcccagga
    19321 tcttttgtcg cgagatcgaa aacggcgaga gtgaaaccgg tgaggtcgac cagaacgacc
    19381 cggtcccgag gccggtgccg gaggccgagg cgaacacttt ctcggcggcg atgttgatgc
    19441 cgatcgacca ggttcggaag agactttcct ccaacccggc cgacccggtc aaggatctcc
    19501 aggactattt cggcgtcaac cggaagccgg cgcttcgacg ggtcgaaacg gcgcggattc
    19561 tcggctgaac gaaccgggcg gctacttccg gacgttcagc tttgcggagg ccggccggaa
    19621 tccgtctttc gccaccttga cggtgagctt gccggactgc ttcggcttga ccttcagggt
    19681 tgccttgccg ttgtaggcgg tgatggccga ggcgccgccg gcttcgacct tgaccgcgtc
    19741 gaccggcttc gtcactccgg tgtagttcat gcccttgacg gtcacggtga tcgtgccggt
    19801 cctgcccttg cgcagtccgc cgcccttcgc ctgcagcttc agactgcggc agggcttctc
    19861 cgacagggtg cgggtgaagg gcaggtcagc cgagaacgag cagccgttgc cgtcgaagat
    19921 cgtcatttcg ccctcgccgg tcaccttgag cctgtttccg gtccgcttga aggtggtgac
    19981 tgcggtcggg gccgggctga agttgaagta gatgtcccag gcctggcctt cggaggcggc
    20041 ggtgcggtag gaccagttgg ctggccgggc gggggtgggg cggaacaagc ccttggcgat
    20101 cgccgctttc aggtcggggc gccagaactc ccagtcgtgg ctgcccttgt ggacggtgta
    20161 gtcgacgttc ttgttcccgg aagcctccat ggccgccacg gccgcatcgt tttgcgtctt
    20221 cagcacactt tcgaggtaga ggctgacccc gtcgccatcg gtcacgtagc cagggttgga
    20281 cggatcgttc tgggggcgga cctccgggtc gggttcaccg tcgccggtga acacgtcgag
    20341 attggtgaag gcgaggttcg gtccaagcgc gatcggatca tgcccttccg cgtagaaacc
    20401 ggtcgggtcg ccgtacacct gggcgtaagg ggtgccgccg gccgccgtcg cgaaggcgag
    20461 ggtcgattca aaggtgcgga tcgaggcgaa ggccgatagc ggcaccgccg tgccgaagaa
    20521 tcccggcagc tgcgaggcgg tcagccaggt gccgtagccg cccatcgaga atccggcgat
    20581 cgcgtggtag cggcgctcgg gccggatgct gaacctgctc tggatcatcg gcagcaggga
    20641 ctcgcggatg tagtcctccc aggccgggcc actgcgctcg ccttcgttcc accagtcggt
    20701 gtagaaaccc tgcgcgcctt ccggcatgac gatcaccgcg ttcaggccct tcgcggtgtt
    20761 cctgatgtcc ccgagcagcg ggtctgccca ggagtcgaac ctttcgccgg aaccgtgaag
    20821 gaggagcagc agcgggtagc gcttcttcgg cgtgtaaccg tccggcagca gcacgttggc
    20881 catcagcttg ccggggtggg ggcccatgtt ttcggtcacc ggaccagcca gatgctggtt
    20941 cgagggatcc acatagtcgc tggaagcatc gatcgtgacc agcttctgcg ccgaagcagg
    21001 agcagccagc atcagcatgg cggcaaaggc ggccgcggca aacgccacca gcttccaggc
    21061 agcactgaac gaacgacttc tccccaacgt catggttccc ataggcggca tggtaagccc
    21121 ttgcgcgaga accagccagc tcacctgtgg ttgagggtgc cggccaggcg gggggcgagg
    21181 gaggagtagc ggcggccggc ttcgtcgcgg cggacgccgt tttcgagggc ggggcggtct
    21241 cccacca
//
LOCUS       DAOQKN010000017        20993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_1094_length_20993_cov_6.115006, whole genome shotgun sequence.
ACCESSION   DAOQKN010000017 DAOQKN010000000
VERSION     DAOQKN010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 20993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20993
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1094_length_20993_cov_6.115006"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(573..833)
                     /locus_tag="PLJ64_02260"
     CDS             complement(573..833)
                     /locus_tag="PLJ64_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92350.1"
                     /translation="MSNEPEQTPSARIGALAQELMEQVERDTPGARLDQLVISAVLSE
                     DAGPDEQTMHVQLVSDSSDPLSVVGLLTVALELAKGQLTGGE"
     gene            898..2922
                     /locus_tag="PLJ64_02265"
     CDS             898..2922
                     /locus_tag="PLJ64_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92351.1"
                     /translation="MLRPGAALLAAITCLATLALTVPATSAADDAPRAENTAILVLIP
                     QGPGSPPSYSEEIADGFAGIPQLSVGLTSATQGSYRREQALLDISQGTRVSRSTYDPS
                     EVPPVGLAATVGGGGRVKGWDAILDRAETAPQTIEPGLLAGSIPGGAAYVGNATRFQE
                     TVIPATNRVGRVSALAPAGSGDAVHRAISQSRKYRLVVVAASPGREGLRELRRILQLR
                     RPGQLVIATQTPPLAPILPLLPIAAAGLGENPGDPIETEGKGLRSDTTNLPNLVAGID
                     IAPTILDHLGLSIPDEMTGKPFRAEGSRDPESLTPFRDRLDELGSRRNAALAFVFVGW
                     LIIYLAAGAIAGREKIAHRVRRIGGLSILWIPTVILVPAALGNPSAAVEYLLIAGLAL
                     TLGFLSDRFVPWPRATLVPAVVGLSVITIDLVFGSHLITRSILGPNPGYGSRFYGIGN
                     ELKPALMVLLLAGLAAALTGKPKSKKNAAIVLGFGLGLGVVLGSGRLGAGVGAAIIVA
                     SATAVAALMMLPGKMTVKRGAILIAAPVIGLALLAVLDLTTAGGQGHYSSVLSGGGDS
                     FLDDIRRRTTLAWQQLWNDTMPVITLVCLLAAAYAIRNRDMFRPWAGPVWPAALVGGL
                     AGGLIGSFTEDSGPLLIVVATITLTGVCSYLLGRPEDAGVASGDIPPDKE"
     gene            2925..3293
                     /locus_tag="PLJ64_02270"
     CDS             2925..3293
                     /locus_tag="PLJ64_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNC92352.1"
                     /translation="MAVTGTDFVTIPIQDFEASKAFYRDTLGLEEGKQWGNMPAQEFE
                     TGNLTIAIMDPTAFGQDFRPAGGSSVVLQVDDFEASKAELEGKGVKFVTDTIDSGICH
                     QAYFTDPDGNVLGIHHRYAN"
     gene            complement(3386..3472)
                     /locus_tag="PLJ64_02275"
     tRNA            complement(3386..3472)
                     /locus_tag="PLJ64_02275"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3436..3438),aa:Ser,seq:cga)
     gene            3553..3641
                     /locus_tag="PLJ64_02280"
     tRNA            3553..3641
                     /locus_tag="PLJ64_02280"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3587..3589,aa:Ser,seq:gga)
     gene            complement(3650..4177)
                     /gene="tadA"
                     /locus_tag="PLJ64_02285"
     CDS             complement(3650..4177)
                     /gene="tadA"
                     /locus_tag="PLJ64_02285"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HNC92353.1"
                     /translation="MNLEQESGDLAPGSRNFGPESSDPVLRAGDEEMMRLALAAARKA
                     AEHDDVPIGAVIARDGIPLAMAGNRREADRDPTAHAELIAIRAAASELGGWRLPETTL
                     FVTLEPCAMCAGAIVLARIPRVVFGATDPKAGAAGSIMNVLDDPALNHRPEVESGLMA
                     EECGNLLREFFSARR"
     gene            4290..5726
                     /locus_tag="PLJ64_02290"
     CDS             4290..5726
                     /locus_tag="PLJ64_02290"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNC92354.1"
                     /translation="MNRSAHLSLALFGALLAATCAVVLMLPTAAPADETAPPGTPVSD
                     APADQVAGQATTVLDDGSTAVAGEVLVGHYGEAAPTTVEIPESADPAMVAERIEDSRE
                     VRYAIPNYVASASGWLPDDNGVNPRRKGSWGGWRDKQWNFLPCLGLCHGSLPDDRPQS
                     RGGMNVIRAWQNLRRAGRPGAAGVKVAVIDSGIAYRNYGSRFRRAPDIARNRFLPGYD
                     FVDRDRLPLDLYGHGTHVSMTIGEQTDNRVGLTGIAYRSKLMPVRVLDAYGDGDTNDI
                     VSGVRWATSHGARVIVMSLNFACGVDVPPLDDALRQAWRKGVVVVGSAGNIGAEGCPS
                     LPATGPGVIAVGGSTESGCLANYSFESTLIDVAAPGGGRDRASCPWNSRNRPILQLGM
                     VTRDPSWFSIEPTWIGTSMAAAHVAGAAAAVIASGVLGNRTGPGRVTERLESTARLPA
                     NAIGNPTSGLGAGIVDLGRATNPAVSTG"
     gene            complement(5785..6234)
                     /locus_tag="PLJ64_02295"
     CDS             complement(5785..6234)
                     /locus_tag="PLJ64_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNC92355.1"
                     /translation="MSLFKRIVVGTDGSESAREAIRQAAELARMSGASLDVVSAFEPV
                     PDRRHQEEASGAPADVAHEFGPREDVNFALDGAASIAAGFEVEIARHPMDGDPADAIL
                     KVAEEVDADLIVVGNKGMTGARRFLLGSVPNKVSHHAPCSVFIARTT"
     gene            complement(6287..6436)
                     /locus_tag="PLJ64_02300"
     CDS             complement(6287..6436)
                     /locus_tag="PLJ64_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92356.1"
                     /translation="MPANWRILMIVICVFLVASVTIGAIQLLSTPTEIFGAGFEGLPK
                     QEFPR"
     gene            6487..7230
                     /locus_tag="PLJ64_02305"
     CDS             6487..7230
                     /locus_tag="PLJ64_02305"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNC92357.1"
                     /translation="MNANRVAPKGLEEVADGVWLLTGDIKGAMNIYFIEGPDGEIVQF
                     DAATKSMVKANLRVIREFGGIDRVVLGHAHADHRGTAPYLGVPVHCHPDEVADATSSA
                     SIAPYMYLKELEFAPVRWIYPFLLRRWDGGPVEIAGTVEEGDEIAGFKVIHFPGHAPG
                     LIGLWRESDRVALVSDTVYFVDSARMRPLPEGEASVPHPAWAWDHQKAKESVRKLAGL
                     GPKVVAAGHEHPLRGEDLTDVLLRAADRH"
     gene            7272..9689
                     /locus_tag="PLJ64_02310"
     CDS             7272..9689
                     /locus_tag="PLJ64_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92358.1"
                     /translation="MLLIVAGSLVTGRAITRACGQEKWWGIEPAVGFAALMAVEGLLA
                     RVPGTRAALIAGLVVLAAASLWILRRPRREDLPGSPFFWVAGLIAALLLTIPFAVTGH
                     WGLLGMGYNNDLGLHLAWAEWLRSGFGTEPSDGYPLGPHGLAASLSTIPDFALGRVFI
                     GQVAAIAVLTVMTAWAAVEPLGRWRRLLAAILVGLPYLMVSYFAQAAFKELAAAMFLL
                     AFVIALPRLTPLPAERRLRAAFPLIVLLLGIVFTYSFPGLAWPAAALLAWLLADPDFR
                     ARLKPASIWNWLKRPAVWISVLVSAGLVAALAFVGPFGFGDAFSEVAASNAFGPVSVL
                     EGLGIWLTSDYRLAGNLDTPVPAVLGALSVLALLVSLWWWRKEPRSPYPLAFLACAVL
                     YLISLPWVGDYSLAKALVIAAPVTMVVILTALLSGPRGGWKPSQGMDFGAWVTLATLF
                     VIGAAASSLIVLRDASVSPPGHAKELGAFKREVKGSTVLYADQDRFAPYYLTGAKVSV
                     PLADFPDPDIVENPKKPFQGDTGQGVIDFDSFEGESLQNFDYVITSSASFQSEAPAFF
                     EEVDRTNSYILWKRIDDAYNRPILNEATLPGRLVDCSKGGGQYFSTEVDGTATLMPRT
                     VLALRDQWTPSPDLHQGDSASLTLDLTPGFWWISMQYFTPMGFKLTTDQGFERFFKPA
                     LDGQRLVNQETGSNGQYWPAGVIEVKKAGPVTFTAKSAEPTAIQRLSGYSRITKLGRV
                     AAMRGQGRWKVAMNQICGQWVDWFKRIPISIFRQNDTRQKEAARRQTLREAARFNRVE
                     GSGAVGD"
     gene            9794..11257
                     /gene="zwf"
                     /locus_tag="PLJ64_02315"
     CDS             9794..11257
                     /gene="zwf"
                     /locus_tag="PLJ64_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HNC92359.1"
                     /translation="MADIGPLDVAPTALTPPEPHVVVLFGATGDLSRRKLLPGLFHLD
                     RAGHLSDCRIVGTSPEEMTNAGFRRLARESCESFGRGRVEEDDWNRFERRLSWVSGTT
                     GAAGLSDAVDRAEADIGEQCRRLHYLSVPPAAAPAVIKTLGEAGLVERSRIIMEKPFG
                     TDLESARELNAMVHEVFDEDQVFRIDHFLGKEAALNILALRFANGLFEPIWSRNFIDH
                     VQIDVPETLTVEGRGGFYDATGAFRDMVVTHLFQVLAFVAMEPPVELAPDAITVEKNK
                     VFRSMKGIRPEDVVRGRYEGYHQESGVAEDSDAETFIALKCGIDNWRWSGVPFYLRTG
                     KAMSEGARIISIAFREPPKSMFPEGSGVGMKGPDHLTFDLDESSRISLSYYGKRPGPG
                     MLVDKLSMQFSLNEIETQGDTLEAYERLIRDAMGGDHTLFTTAEGIEELWNTARPLLE
                     NPPGVLTYERGSWGPREIHDLIEPNRWRLPFQRRWRG"
     gene            complement(11307..13079)
                     /locus_tag="PLJ64_02320"
     CDS             complement(11307..13079)
                     /locus_tag="PLJ64_02320"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HNC92360.1"
                     /translation="MDDVLLGLSLVVVLSILARLSATWMRVPPIVPLLIVGMAIGISG
                     LDLVNPSEMLGPALSPAVQIAVGIILFEGALSLKREELGSGVRSAVTRLITLGVVITW
                     FVATVSIALLLDVPHEMSILIGATVIVSGPTVVIPILNFVGPTQKVRRVLKWEGILID
                     PIGAIIAVVVFGSFDNGTGGEFDIGELFLSLGVGGLIGAAGAAVMIPILGTKRLAGRD
                     KVAATLMMVVAAFAASDAVLSDSGLVATIVMGVVLANQHRVRVDQVAEFKETLVPILV
                     GILFVLLAANVDMGQVIDLGWQGLLVIAALIVIARPLAALTLVGLPFTWREKVFFGSM
                     APRGIVAASTASAFGLELTEREVPGAEYVIPVTFLIIIGTVFYYSLLTPWLAKVLGLA
                     GEHRPTLMMIGAPPWALTIGEGLTQAGAEVLVWTESVEDAEAAARRGLVSFGGPLDPE
                     EHEADSPLEDVAAFAVVSEDDTLNQLLSFQLAEIYEANQVYRRRSPSDSSPIVRTEAL
                     ELFHDGDTETEVQKRLSAGEPVSVVGPLARVPEGAIPIAAVIPRRGTMPAKIHLATED
                     KPIKADGRSTLLVLNAVGSSPDGQ"
     gene            complement(13072..13722)
                     /locus_tag="PLJ64_02325"
     CDS             complement(13072..13722)
                     /locus_tag="PLJ64_02325"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HNC92361.1"
                     /translation="MGAERYGFALLLILASMIFIMATHDGKWSNVISLNLQAFALFAS
                     TRAAQPRMAIRVTVGVIIGLVLVAAWIQAGFTSSVNDDFVRLSTFSLVVIATPAIAWG
                     LVRQVKARGMITTHTMLGVLCIYLLMSLAFSAAFGAISAVSGDPFFQQGAADGTTNNY
                     LYYSLTTITTVGMGDFTPATNLGRSLTAAEALIGQIYVITVVAVIVGNLGRPRNNG"
     gene            complement(13737..14411)
                     /gene="pgl"
                     /locus_tag="PLJ64_02330"
     CDS             complement(13737..14411)
                     /gene="pgl"
                     /locus_tag="PLJ64_02330"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HNC92362.1"
                     /translation="MVTRFVEDDESAAAEAARIVELAAEAAIADRGRFNFAVSGGKTP
                     WRMLELLAESDLNWTRTSLFQVDERIAPTGSMQRNLTHLVLTLPLICQAAIRPMPVGA
                     DDLATAASGYEYSLPDRFDLIHLGMGTDGHTASLVPGDPILEVSDRQVAITSGEYQGT
                     RRMSLTYKALDRARQILFLVTGDDKEEALAKLVDSDPSIPAGRLKNPNVTLITDIDIR
                     DSEPVS"
     gene            complement(14412..15443)
                     /gene="gnd"
                     /locus_tag="PLJ64_02335"
     CDS             complement(14412..15443)
                     /gene="gnd"
                     /locus_tag="PLJ64_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HNC92363.1"
                     /translation="MEIGIVGLGRMGANIARRLMRDGHRCVVYDVNPEAAKDLAGEGA
                     DPAADLAELAAKLAAPRAVWLMVPAGDITRDTIRKVAAELQPGDAIIDGGNTYYHDDI
                     HFAEELGKQGIDHLDCGTSGGVFGLERGYCLMIGGPEEAYKRLEPIFATLAPGVGEAE
                     RTPGLTGDPSPAENGYLYCGPAGAGHFVKMVHNGIEYGMMAAIAEGLNVIKGANAGVI
                     TRDGDAETAPMENPEYYQYEIDVSQVAEVWRRGSVVGSWLLDLTAASLAESPTLEEFS
                     GRVSDSGEGRWTSIAAIDEGVPTPVLTAALHERFYSRGLGDFGDKVLSAMRKQFGGHD
                     EKPAEGKEG"
     gene            15500..16936
                     /gene="zwf"
                     /locus_tag="PLJ64_02340"
     CDS             15500..16936
                     /gene="zwf"
                     /locus_tag="PLJ64_02340"
                     /inference="COORDINATES: protein motif:HMM:NF009492.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HNC92364.1"
                     /translation="MSKTKEREATDALVIFGITGDLARKMTFRALYRLEKRGKLDCPI
                     IGVGRNQDWHHETMKQRAEEAIRGSVDEFDLATFRRLADRMRFVNGNFDEEDLYQSLG
                     GELADYERPLFYLEIPPPLFSEVVHGLANAGLVENARVILEKPFGHDFDSAQELQADL
                     LTVLEEDQILRIDHYLGKEPVMDITYLRFANALLEPVWNRNYVSYVQMTMSENFGVED
                     RGRFYEGVGAIRDVVQNHMLQVLALLAMEAPNANQHDSIRAKKLELFEAIRSADPAKC
                     VRGQYNGYRDIPDVADDSQVETYAALELAIDNWRWSGVPFYLRTGKNLPVKHTEVRVV
                     FKRPPHTGVGMKSHKPPRNQMVVRIDPVPGARMAFVAKAAGIEDFEEADLDVLFEKEP
                     GAEPEPYERLLDDAIHGRYDLFTKFSMVEETWRIVQPLLDDPPPVVPYEVGSWGPEEA
                     DRLVKGICDWDEPWRPTEDPRHHMLLGG"
     gene            17008..17499
                     /locus_tag="PLJ64_02345"
     CDS             17008..17499
                     /locus_tag="PLJ64_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HNC92365.1"
                     /translation="MTEILNVLVEVPKGSRNKYEYDEELGAIKLDRFLFSSMVYPVDY
                     GMVPNTMGADGDPLDAMVCVSEPTFPGCIIPAIPLGLFRMTDDGELDDKILCVPADDP
                     NWNFIESLDQLSSQLKDEISHFFAVYKQPEGKHVEVDGWYPLESALEVLELSRERFAS
                     GTA"
     gene            complement(17609..18598)
                     /gene="rlmN"
                     /locus_tag="PLJ64_02350"
     CDS             complement(17609..18598)
                     /gene="rlmN"
                     /locus_tag="PLJ64_02350"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNC92366.1"
                     /translation="MDLTLLEETMAGLEQPAYRTGQVWEWLARGAGSFDEMTNLPASL
                     RQELEGRLPISTLEVDASMKSVDGTEKALFLTADGRPVEAVLMRYRDGRRSLCLSSQS
                     GCPLTCTFCATGTMKFGRNLTESEILDQALHFRRIEPVNHAVFMGMGEPMMNLDNVLA
                     VCERLPEVGIANSHTTVSTVGWQPGIERMTTEGPAVRLALSLHAPNDKLRSEIMPVND
                     RYPMEDVVAACREWRHTRKRKVFIEYLMLDGVNDSVELAHELADLLLPKNDFKVNLIP
                     YNPSGTGYQGSPREAIDRFRDTLMKRGIHATVRLTRGRDIDAACGQLAAKAAA"
     gene            complement(18619..19326)
                     /locus_tag="PLJ64_02355"
     CDS             complement(18619..19326)
                     /locus_tag="PLJ64_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92367.1"
                     /translation="MAGMEENEEKAPRPVAVWVGGTLAYAVLVLALGLILTGINPDSS
                     VFPPLLTALAGLFVATAINVRPASSGDADPDWNRFQVHVIVVAITSMVLSIGLVMCAL
                     AFPGELIFQLAFWGAALAILIGAAFLLAVVWERLSTEGSPPSPASEPEPKPDPDPEPE
                     PEPEPEPAPEAEPAPESGKVKAAKKELRRAPQTDLVAATLKKSKKTKKPAKKSPKDPK
                     ASKAPKKPKKKDSKKKK"
     gene            complement(19326..20450)
                     /locus_tag="PLJ64_02360"
     CDS             complement(19326..20450)
                     /locus_tag="PLJ64_02360"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HNC92368.1"
                     /translation="MNALDGLLVADFSRVLAGPLVSMTLADFGADVIKVEPPGGDETR
                     AWSPPVDAQGRATFFLAVNRNKRSVELNLKDPEDLELAKELVGRADVLVENFRPGTMD
                     RLGLGYEEMAAANPGLVYCSISGFGSKEGAALPGYDPLVQAMSGMMSVTGPPEGDPSK
                     VGVALVDVIAGLNATIGVLIALQERAKSGQGQKVESNLLSSALAALENQASAWLNTGV
                     LPVRLGNVHPSIEPFTTFDASDGPLMICVGNDRQFKALCEVLGEPELSSDPRYVDNFD
                     RVEHRVELEAELNELFSRRTVEEWVTALHEARVPAGPVNTIDRGFAVAEALGLDPIDE
                     IDGVRTPASPIRLGRTPAETRLPPPAENAHSEEIRDWLRS"
     gene            complement(20520..>20993)
                     /locus_tag="PLJ64_02365"
     CDS             complement(20520..>20993)
                     /locus_tag="PLJ64_02365"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HNC92369.1"
                     /translation="TVEEVLRTGIQLASAVETAHRAGIVHRDIKPANVLVSQYGAPGL
                     TDDPRFATNLDRVENRAPLREELERRLGLDSVEYWAEALNDAGVPAGPVNDIGGGFGL
                     AGNLELDPVTEIEGTRTTSSPIRLSGSPPERRFPPPDLDQHGEEIREWLHAPREE"
BASE COUNT         3481 a         7019 c         6908 g         3585 t
ORIGIN      
        1 accgcagcga catcgccgct ccgatcgaag agcgcctgat cgtcgagcac tactctcgct
       61 aggccgcgag caaccgcaga gtttcagggc cccgggaacc ggggcccttt ttcttgcacc
      121 cgagccgtcg aggatttgat tcgcatgtct cgtgggtcgg ttttccggcc cgactgcgga
      181 gaatccgacc cactgcggtg gtgcagggca ggcgaagggg gacgttggag ggatccccgg
      241 gtccgagctg gctccggggc cggactcgcg tccctatcca ggcggtgccg caggtttgca
      301 acctctgggg ggggaaacct gcggcaccgg ccaccgagtc acggtcaggg atcggtcgag
      361 atcgggtctg tcggcgatat atgccgacaa accgcatctc gcgaaatgcc aacaccacca
      421 gccgtaagcg ggtcgtgtcc aggggcctcc agccgtgcgc tggcaaggag acaggccgga
      481 aatgaccgcg atagtacgcg ggtacgtgag ctgtcgtttc cggccgccga cgaagtcagc
      541 gtgcggctgg tggttcctgg gcggggcccg ggctactcgc ctccggtcag ctgacctttg
      601 gctagttcca gagcgaccgt gaggaggccg actacggaga gggggtcgga ggagtcggaa
      661 accagctgga cgtgcatcgt ctgctcgtcc ggtccggcat cctcgctcag gacggccgag
      721 atcacgagct ggtccagtcg tgccccgggg gtgtccctct ccacctgctc catcagttcc
      781 tgggcaagcg ccccgattcg tgccgacggc gtctgctccg gttcattgct catgcagcca
      841 ccctaccgcc cggcgcgcca cagctaatcc ccgcaattct caaagtaaac tggccctgtg
      901 cttcgccccg gcgccgccct cctcgcggcg atcacctgcc tggccaccct ggccctgacc
      961 gtccctgcca cgagcgcagc ggatgatgcc ccccgcgcgg agaacacggc gatcctcgtc
     1021 ctgatccccc aggggcccgg atcccctccc tcctatagcg aggagatcgc cgatggcttc
     1081 gccgggatcc cgcagctctc ggtcgggctg acctcggcca cccagggttc ctaccgcagg
     1141 gagcaggcgc tgcttgacat cagccagggc accagggttt cccgctcgac ctacgacccg
     1201 tcggaagtgc cgccggtcgg cctcgctgcg acggtcggcg ggggcggcag ggtcaaggga
     1261 tgggacgcga ttctcgatcg ggccgagacc gcgccccaga caatcgaacc gggactgctc
     1321 gccggttcga ttccgggtgg tgccgcctac gtcggaaacg cgaccaggtt ccaggagacg
     1381 gtgatcccgg ccaccaaccg cgtcggccgg gtgtccgcgc tggccccggc aggcagcggc
     1441 gatgcggtcc accgggcgat cagccagtca cgcaagtacc ggctggtcgt ggtcgccgct
     1501 tcccccgggc gcgaaggact gcgcgagctg cgccggatcc tccagttgcg ccgtcccggc
     1561 caactggtga tcgcgaccca gaccccgccc ctggccccga tcctgccgtt gcttccgatc
     1621 gccgcggccg gcctgggcga gaaccccggc gacccgatcg agaccgaagg caagggcctg
     1681 cgctccgata cgaccaacct gccgaacctg gtcgccggga tcgacatcgc cccgaccatc
     1741 ctcgaccacc tcgggctgtc catcccggac gagatgaccg gcaagccgtt ccgggccgaa
     1801 gggtcgcggg accccgaatc gctcaccccc ttccgtgacc ggctcgacga actcgggtcg
     1861 cgccgcaacg cggcgctcgc cttcgtcttc gtcggctggc tgatcatcta ccttgccgct
     1921 ggcgcgatcg ccgggcgcga gaagatcgcc catcgggtac ggcggatcgg cggcctctcg
     1981 atcctctgga tcccgacagt gatcctggtt ccagccgccc tcggcaaccc gtcggccgca
     2041 gttgagtatc tgctgatcgc gggcctcgcc ctcacgctcg gcttcctctc ggaccgcttc
     2101 gttccctggc cccgggcaac gctggtcccg gcggttgtcg gtttgagcgt gatcacgatc
     2161 gacctggtct tcggttccca cctgatcacc aggtcgatcc tcgggccgaa ccccggttac
     2221 ggatcgcgtt tctacgggat cggcaacgaa ctcaaaccgg ccctgatggt gctgctgctg
     2281 gccggtctcg ccgccgccct gacgggcaag ccgaaatcga aaaagaacgc tgcgatcgtg
     2341 ctcggtttcg ggctggggct cggtgtggtg ctcggctcgg gccgcctcgg cgccggtgtc
     2401 ggagccgcga tcatcgtcgc ctcggcaacc gcggtcgcgg ctctgatgat gctgcccgga
     2461 aagatgaccg tgaaaagggg ggcgatactg attgccgcgc cggtcatcgg tctggcgctg
     2521 ctggccgtgc tcgacctgac tactgccggg ggtcaaggcc actacagctc ggttctttcc
     2581 gggggcggtg acagtttcct cgacgacatc cgccgtcgca ccaccctcgc ctggcagcag
     2641 ctctggaacg acaccatgcc ggtgatcacc ctggtctgcc tgctcgccgc cgcctacgcg
     2701 atccgcaatc gcgacatgtt cagaccatgg gccggcccgg tctggccggc ggccctggtc
     2761 ggcggtctgg ccggaggcct gatcggttcc ttcaccgagg actcgggacc actcctgatc
     2821 gtggtcgcga cgatcaccct gaccggggtc tgctcgtatc tgcttggacg gccggaggat
     2881 gccggggtag catcggggga catcccccca gacaaggagt gaaagtggct gttacaggta
     2941 ccgacttcgt gaccattccg atccaggact tcgaggcttc gaaggctttc taccgcgaca
     3001 ccctcggcct cgaggagggc aagcagtggg gcaacatgcc ggcccaggaa ttcgagaccg
     3061 gcaacctgac gatcgcgatc atggacccga ccgccttcgg ccaggatttc cggccggccg
     3121 gcggtagcag cgtcgtgctg caggtcgatg acttcgaagc ctccaaggct gaactggaag
     3181 gcaagggcgt caagttcgtc accgacacga tcgacagcgg gatctgccac caggcctact
     3241 tcacggaccc ggacggaaac gtcctcggaa tccaccacag atacgcgaac tgagaccgct
     3301 cccggaatca gtgagatccg ggtttttcgt cacataggcg aaaagatcgg atctcgatga
     3361 agagaggacc ttacggcggc ttatacggag ggggtgggat tcgaacccac ggtacgccga
     3421 aacgcacaac agttttcgag actgcccgat tcaaccactc tcgcacccct ccgggggtga
     3481 cttggaaggc tagcgattcg cgcgggacgg ggcgccttcg gcggctgttc tcgggtaccc
     3541 ttccctgtcc ccggagaggt gtccgagcgg cttaaggagc acgattggaa atcgtgtggg
     3601 cgggatctct cgcctcgtgg gttcgaatcc caccctctcc gttccggggt tagcgacggg
     3661 ccgagaagaa ctcgcggagg aggttgccgc actcctcggc catcaggcct gactcgactt
     3721 cgggccggtg gttgagggcc gggtcgtcaa gtacgttcat gatcgagccg gccgcccccg
     3781 ccttggggtc ggtggcgccg aagaccaccc gggggatgcg ggcgaggacg atcgctccgg
     3841 cacacatcgc gcatggctcg agggtcacga agagcgtggt ttccgggagg cgccagccac
     3901 ccagttcgga ggcggccgcg cgaatcgcga tcagctccgc gtgcgcggtc gggtcccggt
     3961 cggcttcgcg ccggttcccg gccatagcca gggggatgcc gtcccgggcg atcacggcgc
     4021 cgatcgggac atcgtcatgt tccgctgcct tgcgggcggc cgcgagggcc agccgcatca
     4081 tctcctcatc gccggcccga agcacgggat cactgctttc agggccgaaa ttgcggcttc
     4141 cgggtgccag gtcgccgctt tcctgttcca ggttcaccgg ttcagggtaa gtcatggggc
     4201 agggcgaggt tccgcccttt tcgggagttc gagcaacccg gcggggtttg gggtgttctt
     4261 tcagtaccgg tcgcctactg ccttcttcct tgaatcgatc agcccacctc agcctcgcgc
     4321 ttttcggcgc cctgctggcc gccacctgcg cggtcgtcct gatgctgccg acggcggccc
     4381 cggctgacga aaccgcgcct cccgggacgc ccgtttccga cgctccggcg gatcaggttg
     4441 ccggccaggc gacgaccgtg ctcgatgatg gttcgactgc ggttgccggc gaggtcctgg
     4501 tcggccatta cggcgaggca gcaccgacga cggttgaaat cccggaatcc gcggacccgg
     4561 cgatggtcgc cgagcggatc gaggattccc gcgaggtccg ctacgcgatc ccgaactacg
     4621 tcgccagtgc ctcgggctgg ctgccggatg acaacggagt caatccccgc cgcaagggca
     4681 gttggggcgg ctggcgggac aagcagtgga acttcctgcc ctgcctcggt ctctgtcatg
     4741 gctcgctgcc tgacgaccgg cctcagtccc gtggcgggat gaacgtgatt cgcgcctggc
     4801 agaacctgcg ccgggctgga cggccgggcg ccgccggggt caaggtcgcg gtgatcgaca
     4861 gcggcatcgc ctatcgcaac tacggcagcc ggttccggcg ggcaccggac atcgcccgca
     4921 accgctttct gcccggctac gacttcgtcg accgtgaccg gctgccgctc gacctctacg
     4981 gtcacggaac ccacgtctca atgacgatcg gtgaacagac cgacaaccgg gttggcctga
     5041 ccggaatcgc ctatcgctcg aagctgatgc cggtgcgggt gctggacgcg tacggcgacg
     5101 gcgacaccaa cgacatcgtc tccggtgtgc gctgggcgac cagccacggc gcccgggtga
     5161 tcgtgatgtc actgaacttc gcctgcggag tcgacgtgcc gccgcttgac gacgctcttc
     5221 gccaggcctg gcgaaagggc gtggtggtgg tcggctcggc cggcaacatc ggggccgagg
     5281 gctgcccctc cctgccggcc accggacccg gggtcatcgc ggtcggtggt tcgaccgaat
     5341 ccggctgcct ggccaactac tccttcgaaa gcaccctgat cgacgttgcc gctcccggcg
     5401 ggggaaggga ccgtgcaagc tgcccctgga actctcgcaa ccgcccgatc ctgcagctgg
     5461 gcatggtcac ccgtgacccg agctggttca gcatcgaacc gacctggatc ggcacctcga
     5521 tggcggccgc ccacgtggcc ggagccgccg cggcggtgat cgcttccggc gtgctcggca
     5581 accggaccgg ccctggccgg gtcaccgaac gcctcgaaag caccgcccgc ctgccggcca
     5641 acgcaatcgg taacccgacc tcgggactgg gggccggaat cgtggatctc ggccgcgcca
     5701 ccaaccccgc cgtcagcacc ggctgaaccc ggagcgccgc gggacgcggt gcgcccgaac
     5761 cggctgtcag ccggcgagca ggcgctaggt ggttcgggcg atgaagacgc tgcagggggc
     5821 gtggtgggaa accttgttcg gcaccgaacc gagcaggaaa cggcgggccc cggtcattcc
     5881 cttgttgccg accacgatca ggtccgcgtc gacttcttcg gcaaccttga gaatcgcatc
     5941 ggccggatcg ccgtccatcg gatgcctcgc gatttccacc tcgaacccag cggcgatcga
     6001 ggccgccccg tcaagggcga aattcacgtc ctcgcgggga ccgaattcat gtgccacgtc
     6061 agccggcgcg ccgctcgcct cttcctggtg acgccggtcc ggaaccggct cgaaggcgct
     6121 cacgacatcg agcgaagccc ccgacatccg ggccagttcg gccgcctgac ggatcgcttc
     6181 gcgggccgac tcggaaccgt cggtaccgac aactatgcgc ttgaacaggg acattggtct
     6241 ccgatcgtcg tgggcggtca ccggcatcct acccccggga ccagtcctat ctgggaaact
     6301 cctgctttgg caaaccctca aaaccggcac cgaagatttc ggtcggagtg gaaagcaact
     6361 ggattgcgcc gatggtgacc gaggccacga ggaatacgca gatcacgatc atcagaattc
     6421 tccagttggc cggcacggga ttcaggttat ggaaaagacg ctcaagatga caccggaagc
     6481 cagcaagtga acgcgaatcg ggtagcgccg aagggactcg aggaagtggc ggacggagtc
     6541 tggctgctga ccggcgacat caagggcgcg atgaacatct acttcatcga aggcccggac
     6601 ggggagatcg tccagttcga cgcggccacg aaatcgatgg tcaaggccaa cctgcgggtg
     6661 atcagggaat tcggcggtat cgacagggtc gtgctcggcc acgcgcacgc cgaccatcgc
     6721 ggcaccgccc cctacctcgg cgtgccggtc cattgccatc ccgacgaggt cgcggatgcc
     6781 acttccagcg cctcgatcgc gccctacatg tacctgaagg aactcgagtt tgccccggtc
     6841 cgctggatct acccctttct gctgcggcgt tgggatggcg gtccggtcga gatcgccgga
     6901 accgtcgagg aaggcgacga gatcgcgggc ttcaaggtga tccacttccc cgggcatgcc
     6961 ccgggtttga tcggactgtg gcgtgaatcg gaccgggtcg ccctggtcag cgacaccgtc
     7021 tacttcgtgg attcggcccg gatgagaccg ttgccggagg gcgaggcgtc ggtaccgcat
     7081 ccggcctggg cctgggacca tcagaaggcc aaggagtcgg tgcgcaaact ggccggactg
     7141 gggccgaagg tggtggccgc cgggcacgag cacccgctcc gtggcgagga cctgaccgac
     7201 gtgctgctca gggcagccga caggcactag ctccggggcg actggcaggg atgttctaca
     7261 gctggctccc catgcttctg atcgttgccg ggtccctggt gaccggccgc gcgatcaccc
     7321 gcgcctgcgg ccaggagaag tggtggggaa tcgaaccggc ggtcggcttc gcggccctga
     7381 tggcggtcga gggcctgctg gcccgggttc cgggaacgcg ggcggccctg atcgcgggtc
     7441 tggtggttct ggctgcggcc agcctctgga tactgcggcg accgcgacgg gaggacctgc
     7501 ccggctcgcc cttcttctgg gtcgcgggcc tgatcgcggc cctcctcttg accattccct
     7561 tcgcggtgac cggtcactgg ggcctgttgg gaatgggcta caacaatgac ctcggcctgc
     7621 acctggcctg ggccgaatgg ctgcgctccg ggttcgggac cgagcccagc gacggctacc
     7681 cgctggggcc gcacgggctc gcggcctcgc tttcgaccat ccccgacttt gccctcgggc
     7741 gggtcttcat cggacaggta gcggccatcg cggtcctgac cgtgatgacg gcctgggcag
     7801 cggtcgagcc gctcggccgc tggcgccggc tgctcgcggc gatcctggtc ggcctgccct
     7861 atctgatggt ttcctatttc gcccaggcgg ccttcaagga actcgcggcc gcgatgttcc
     7921 tgctcgcctt cgtgatcgcc ctgccccggc tcacgccgct cccggccgag cgacggctgc
     7981 gggctgcctt tcccctgatc gttctcctgc tcgggatcgt cttcacctac agcttccccg
     8041 ggctggcctg gccggcggcg gcactccttg cctggctgct ggccgacccg gatttccggg
     8101 cgaggctgaa gccggcgtcg atctggaact ggctgaagcg gcccgccgtc tggatttcgg
     8161 tcctggtctc cgccggcctg gtcgccgccc tcgccttcgt cggtcccttc ggcttcggtg
     8221 acgccttttc cgaggtggcg gccagcaacg ccttcggccc ggtctcggtg ctggagggtc
     8281 tggggatctg gctgacctcc gactaccgtc tcgccggcaa cctggacact ccggtgccgg
     8341 ccgtgctcgg agcgctctcg gttctcgccc tgctggtctc gctctggtgg tggcggaagg
     8401 agcctcgctc gccctacccc ctggccttcc tcgcctgcgc cgtgctctac ctgatctcgc
     8461 tgccctgggt cggggactat tcgctggcca aggccctggt catcgccgcc ccggtgacca
     8521 tggtggtaat cctgacggcg ctgctgagcg gcccgagagg gggctggaag cccagccagg
     8581 gaatggactt cggcgcctgg gtgaccctcg ccaccctgtt cgtgatcggc gccgcggcct
     8641 cgagcctgat cgtgcttcgg gatgcctcgg tttcaccacc cggccacgcg aaggaactcg
     8701 gcgccttcaa gcgggaggta aagggcagca ccgtcctcta cgccgaccag gatcgcttcg
     8761 ccccctatta cctgaccggg gccaaggtct cggtgccgct ggccgacttc cccgaccccg
     8821 acatcgtcga gaacccgaag aagccgtttc agggcgacac cggccagggc gtgatcgact
     8881 tcgattcgtt cgagggcgaa agcctccaga acttcgacta cgtgatcacc tcctcggctt
     8941 ccttccagag cgaggcaccg gccttcttcg aagaagtcga ccggaccaac tcgtacatcc
     9001 tctggaaacg gatcgacgat gcctacaacc ggccgatcct gaacgaggcg accctgcccg
     9061 ggcgcctggt cgactgctcg aaggggggcg gccagtattt ctcgaccgaa gtcgacggca
     9121 ccgcgaccct gatgccccgg accgtactgg ccttgcggga ccaatggacg ccctcccccg
     9181 atctgcacca gggcgattca gccagtctga ccctggacct caccccggga ttctggtgga
     9241 tctcgatgca gtacttcacc ccgatgggtt tcaagctgac caccgaccag ggcttcgagc
     9301 ggttcttcaa gccggcgctt gacggtcagc gcctggtcaa ccaggaaacc ggcagcaacg
     9361 gccagtactg gccggccggg gtgatcgaag tgaagaaggc cggcccggtc accttcacgg
     9421 cgaaatcagc ggagccgacg gcaattcagc ggctcagcgg ctactcgagg atcaccaaac
     9481 tcggtcgggt cgccgcgatg cgcggccagg gccgctggaa ggtggcgatg aaccagatct
     9541 gcggccagtg ggttgactgg ttcaagcgca ttccgatttc catcttccgc cagaacgaca
     9601 ccaggcagaa ggaagccgca cgccggcaga ccctgcgtga agcggcgcgc ttcaaccggg
     9661 tcgagggcag cggagcggtc ggcgactgag ctcttccggc cagcggcagg gttggaccat
     9721 ggtcgcagcg ccggcctgaa aaacaggaca ggaagttagc cattggtcaa ccgggtaccg
     9781 gttagattcg agcatggccg acatcggacc ccttgacgtt gccccgaccg cgctgacccc
     9841 gccggaaccc catgtggtgg ttcttttcgg ggccaccggc gacctttccc gacgcaagct
     9901 gctgccgggc ctctttcatc tcgaccgggc cgggcatctc tccgattgcc ggatcgtcgg
     9961 cacctcaccg gaggagatga ccaatgccgg gttccggcgg ctcgcccggg aatcatgtga
    10021 gtcctttggc cggggccggg tcgaagagga tgactggaac cgttttgaac gccggctctc
    10081 ctgggtcagc gggaccaccg gcgcggccgg tctgagcgat gcggtggaca gggcggaggc
    10141 agacatcggc gagcaatgtc gccggctcca ttacctgagc gtgccgccgg ccgcggcgcc
    10201 ggcggtgatc aagaccctgg gcgaggccgg cctggtcgag cgttcgcgga tcatcatgga
    10261 aaagcccttc ggcaccgacc tcgaatccgc ccgtgagctg aacgcgatgg tccacgaggt
    10321 cttcgatgag gatcaggtct tccgcatcga tcacttcctc ggcaaggaag cggcgctgaa
    10381 catcctcgcc ctgcgcttcg ccaacgggct cttcgagccg atctggagcc ggaacttcat
    10441 cgaccacgtc cagatcgacg tgcccgagac gctgaccgtc gaggggcggg gcggcttcta
    10501 cgacgcgacc ggcgccttcc gggacatggt ggtcacccat ctcttccagg tcctcgcttt
    10561 cgtcgcgatg gagccgccgg tcgagctggc cccggacgcg atcaccgtgg agaagaacaa
    10621 ggtctttcgc tcgatgaagg gcatccggcc ggaagacgtg gtccggggcc gctacgaggg
    10681 ataccaccag gaaagcgggg tcgccgagga ttccgacgcc gagacattca tcgccctgaa
    10741 gtgcgggatc gacaactggc gctggtccgg ggttcccttc tacctgcgca ccggcaaggc
    10801 gatgtccgaa ggggcacgaa tcatctctat cgccttccgc gaacctccca agagcatgtt
    10861 cccggagggc tcgggggtcg ggatgaaggg cccggaccac ctcaccttcg acctcgacga
    10921 gtcttcgcgg atctccctct cctactacgg caagcgaccc gggccgggca tgctggtcga
    10981 caagctgagc atgcagtttt cgctgaacga gatcgaaacc cagggagaca ctctcgaggc
    11041 ctacgaaagg ctgatccgcg acgcgatggg tggcgaccac acgctcttca ccaccgccga
    11101 gggaatcgag gaactctgga acacggcccg tccgttgctg gagaacccgc ccggggtgct
    11161 gacctacgaa cgcggttcct ggggtcctcg ggaaatccac gacctgatcg aaccaaaccg
    11221 ctggcgcctc cccttccaac gacgctggcg cggctagaaa accggagccc gcccatccat
    11281 tcttttgctc tgtgggcggg cgggtttcat tggccgtcgg gcgaagaccc gacggcgtta
    11341 aggacgagga gcgttgagcg gccgtccgcc ttgatcggtt tgtcctcggt cgccaggtga
    11401 atcttggccg gcatcgttcc gcggcggggg atgacggcgg cgatcggaat cgcaccctcg
    11461 ggaacccggg ccagcgggcc gaccaccgaa accggctcgc cggccgaaag cctcttctgg
    11521 acctcggtct cggtgtcgcc gtcgtggaac agttcaagcg cctcggtgcg gacgatcggc
    11581 gagctgtcgg aaggtgatcg ccggcggtag acctggttgg cttcgtagat ttccgccagc
    11641 tggaaggaca gaagctggtt cagcgtgtca tcctcgctga ccacggcgaa agcggcgaca
    11701 tcctccagcg gggaatccgc ctcatgctcc tcgggatcga gcggtccacc gaaggaaacg
    11761 agcccgcgcc gggccgcggc ctcggcgtcc tcaaccgatt cggtccagac cagcacttcg
    11821 gctccggcct gggtcaggcc ctcccctatg gtcagcgccc acggcggtgc cccgatcatc
    11881 atcagggtcg gccggtgctc cccggcgaga cccaggacct tggccagcca gggggtcagc
    11941 agcgagtagt agaagaccgt gccgatgatg atcaggaagg tcaccggaat gacgtattcg
    12001 gcccccggca cttcgcgctc ggtcagctcg aggccgaagg cggaggcggt tgaagcggcg
    12061 acgatgccgc gcggagccat tgacccgaag aagacctttt cccgccaggt gaagggcagc
    12121 ccgaccaaag tgagcgcagc cagcggccgg gcgatgacga tcagggcggc gatgaccagc
    12181 aggccctgcc agccaaggtc gatcacctga cccatgtcca cgtttgcggc cagcaggacg
    12241 aagaggatcc cgaccaggat cggcaccagc gtttccttga actccgcgac ctgatcgacc
    12301 cggacccgat gctgattggc gagaacgact cccatgacga tcgtcgcgac caggcccgaa
    12361 tcggagagca cggcgtccga ggcggcaaag gcggcgacca ccatcatcag ggtggctgcg
    12421 accttgtccc gtccggccag ccgcttggtt ccgaggatcg ggatcatgac cgcggcgccg
    12481 gccgccccga tcaggccgcc gaccccgagc gaaagaaaga gctcgccgat gtcaaactcg
    12541 ccgccggttc cgttgtcgaa ggatccgaag acgaccaccg cgatgatcgc cccgatcggg
    12601 tcgatcagga tgccctccca tttcaggacc cggcgaacct tctgggtggg accgacgaaa
    12661 ttgaggatcg ggatcaccac ggtcggcccg gaaacgatta ccgtcgcgcc gatcaggatc
    12721 gacatctcgt ggggaacgtc gagcagcaag gcgatcgaga ccgtggcgac aaaccaggtg
    12781 attacgaccc cgagcgtgat cagtcgggtc accgcgcttc tcaccccgga accgagctcc
    12841 tccctcttca ggctgagcgc cccttcgaaa aggatgatgc caaccgcaat ctgaaccgcg
    12901 ggcgagagcg ccgggcccag catctcgctc gggttgacga gatcgagacc ggagatgccg
    12961 atcgccatgc cgacgatcag cagcgggacg atcggcggca cccgcatcca ggtggccgag
    13021 agccgggcga ggatcgagag gacgacgacc aggctcaggc caagtaggac gtcatccatt
    13081 gtttcggggt ctgcccaggt tgccgacgat cacggcgacc accgtgatca cgtagatctg
    13141 cccgatcaat gcctcggctg cggtgaggga acggcccagg ttggtcgccg gggtgaagtc
    13201 gcccatgcca actgtggtga tcgtggtcag gctgtagtag aggtagttgt tggtcgtgcc
    13261 gtcggcggcg ccctgctgga agaacgggtc gccgctgacc gcggagatcg ccccgaacgc
    13321 agccgagaac gcgaggctca tcagcaggta gatgcagagc acgccgagca tcgtgtgggt
    13381 ggtgatcatt ccccgagcct tgacctgccg caccagcccc caggcgatcg cgggggttgc
    13441 gatcacgacg aggctgaagg tcgaaagccg cacgaaatca tcgttgaccg aactggtgaa
    13501 cccggcctga atccatgcgg cgacgaggac cagtccgatg atcacaccga ccgtgacccg
    13561 aatcgccatc ctcggctggg ccgcccgggt tgaagcgaac agcgcaaagg cctgaaggtt
    13621 cagggaaatg acgttggacc atttcccgtc atgagtggcc atgatgaaga tcatcgaggc
    13681 gaggatcaga agtagcgcga agccgtagcg ctcggccccc atttcgcgga tcttcgtcaa
    13741 gaaaccggtt ccgaatccct gatgtcgatg tcggtgatca gggtgacgtt ggggttcttg
    13801 agccgcccgg ccggaatcga cgggtcggag tcgaccagct tcgcgagcgc ttcctccttg
    13861 tcgtccccgg tgaccaggaa gagaatctgc cgggcgcgat caagcgcctt gtaggtcagc
    13921 gacatgcgac gggtgccctg atactcgccg gaagtgatcg caacctggcg gtcggaaacc
    13981 tcgaggatcg ggtcccccgg caccagcgag gcggtgtgcc cgtccgtgcc catgccgagg
    14041 tggatcaggt cgaagcggtc cggcagcgaa tactcgtaac cgctggccgc cgtcgcgagg
    14101 tcatcggccc cgaccggcat cggccggatc gcggcctggc agatcagcgg cagggtgaga
    14161 accaggtggg tgaggttgcg ctgcatgctg ccggtcggcg caatccgctc gtcaacctgg
    14221 aacagcgagg tccgggtcca gttgaggtcg ctttcggcca gcagttcaag catccgccag
    14281 ggcgtcttgc cgccactgac cgcgaaattg aagcgccccc ggtcggcaat cgcggcctcg
    14341 gccgccaact cgacaatccg ggcggcctcg gccgcggctg actcgtcgtc ctcgacgaac
    14401 cgggtgacca attacccttc cttcccctcc gcgggcttct cgtcgtgacc gccgaactgc
    14461 ttgcgcatgg ccgagagcac cttgtcaccg aagtcgccca ggccgcgcga gtagaaccgc
    14521 tcatgcagag cggcggtgag gaccggggtc ggcactcctt cgtcgatcgc ggcgatcgag
    14581 gtccagcgac cctcgccgga atccgaaacc cgcccggaga actcctccag ggtcggggac
    14641 tcggccagcg acgcggcggt gaggtcgagc agccaggaac cgaccacgct gccccggcgc
    14701 cacacttcgg cgacctggga gacgtcgatt tcgtactggt agtactcggg gttctccatc
    14761 ggtgcggtct cggcgtctcc gtcacgggtg atcacgccgg cgttggcgcc cttgatcacg
    14821 ttcagcccct cggcgatcgc ggccatcatc ccgtactcga tcccgttgtg gaccatcttc
    14881 acgaagtggc cagcgccggc cgggccgcag tagaggtagc cgttctcggc cggggaggga
    14941 tctccggtga gccccggggt gcgttccgcc tcgccgaccc cgggggcgag cgtggcgaag
    15001 atcggctcga ggcgcttgta ggcctcttcc ggcccgccga tcatcaggca gtagcctcgc
    15061 tccaggccga aaacgccgcc ggaggtgccg cagtcaaggt ggtcgatgcc ctgtttgccc
    15121 agttcttcgg cgaagtggat gtcatcgtgg tagtaggtgt tgccgccgtc gatgatcgcg
    15181 tcgccgggct gaagttcggc cgcgaccttg cggatcgtgt cgcgggtgat gtctccggcc
    15241 gggaccatga gccagaccgc gcgcggggcg gccagcttgg cggccagctc ggccaggtcg
    15301 gcggccggat cggctccttc accggccaga tccttcgcgg cttcgggatt cacgtcatag
    15361 accacgcacc tgtgtccatc gcgcatcaac ctccgggcga tgttcgcccc catccgacca
    15421 agcccgacga tcccgatttc catgccgcca tattagaacc cgtggtggaa catttcgtcg
    15481 gatggatacg ctgccggtca tgagcaagac caaggagagg gaagcgaccg acgcgctggt
    15541 catcttcgga atcaccggtg atctcgccag gaagatgact ttccgggccc tttaccggct
    15601 cgaaaagcga ggcaagctgg actgcccgat catcggcgtc ggccgcaacc aggactggca
    15661 ccacgagacc atgaagcagc gggccgagga ggcgattcgc ggttcggtcg acgagttcga
    15721 tctcgcaact ttccggcgcc tcgccgatcg catgcgcttc gtcaacggca acttcgacga
    15781 ggaggatctc taccagtcgc tcggtggcga gctggccgac tacgagcggc cgctcttcta
    15841 cctcgagatc ccgccgccgc tcttctccga ggtggtccac ggtctcgcca acgccggtct
    15901 ggtcgaaaac gcccgggtca tcctcgagaa gcccttcggc cacgacttcg actcggccca
    15961 ggaacttcag gccgacctgc tgaccgtgct cgaggaagac cagatcctca ggatcgacca
    16021 ttacctcggc aaggagccgg tgatggacat cacctacctc cgcttcgcca acgcgctgct
    16081 cgaaccggtc tggaaccgca attacgtctc ctatgtccag atgaccatga gcgagaactt
    16141 cggggtcgag gaccgcggcc gcttctatga gggggtcgga gcgatccgcg acgtggtcca
    16201 gaaccacatg ctccaggtgc tggccctgct ggccatggag gccccgaacg cgaaccagca
    16261 tgactcgatc cgggccaaga agctcgagct cttcgaggcg atccgctcgg ccgacccggc
    16321 caaatgcgtc cgcggccagt acaacggcta ccgggacatc ccggacgtcg ccgacgattc
    16381 ccaggtcgag acctacgccg cgctggagtt ggcgatcgac aactggcgct ggtcgggggt
    16441 ccccttctac ctgcggaccg gcaagaacct gccggtcaag cacaccgagg tccgggtggt
    16501 cttcaagagg ccgccccaca ccggggtcgg aatgaagtcc cacaagccgc cgcgcaacca
    16561 gatggtggtc cggatcgacc cggtgcccgg ggcgcgcatg gccttcgtcg ccaaggccgc
    16621 cggtatcgag gacttcgagg aagcggacct cgacgtgctc ttcgagaagg agccgggagc
    16681 cgaaccggag ccctacgagc gcctgctcga tgacgcgatc cacggccgct acgacctctt
    16741 caccaagttc tccatggtcg aggagacctg gcggatcgtg cagccgctgc tcgatgatcc
    16801 gcccccggtc gtcccctacg aggtcggcag ctggggcccg gaggaggccg atcgcctggt
    16861 caagggcatc tgcgactggg atgaaccctg gcgcccgacc gaggatccca ggcatcacat
    16921 gctcttgggc ggctaaagcc accggaggtg ccatggaggg cgggtcggac cccgcctgac
    16981 tccttaaaag ataggattcg cggcgatatg actgagatcc tgaacgttct ggtggaagtc
    17041 ccgaagggct cccgaaacaa gtacgaatac gacgaagagc tgggcgcgat caagctcgac
    17101 cgtttcctct tctcctcgat ggtctacccc gtcgattacg gcatggtgcc gaacacgatg
    17161 ggcgccgacg gcgacccgct cgacgccatg gtttgcgttt ccgaaccgac cttccccggc
    17221 tgcatcatcc cggcgatccc gctcggcctt ttccgcatga ccgacgacgg cgagctcgac
    17281 gacaagatcc tctgcgtccc ggccgacgac ccgaactgga acttcatcga gtcgctcgac
    17341 cagctctcga gccagctcaa ggatgaaatc tcccacttct tcgcggtcta caagcagccc
    17401 gaaggcaagc acgtggaagt cgacggctgg taccccctgg agtccgccct cgaagtcctc
    17461 gaactcagcc gcgaacgctt cgcctccgga accgcataag taagtcgggc ggggtccgac
    17521 ccgccctcca cgagaaaccc gtcccttcct ctcacattca cggatggaag gaagggactc
    17581 cgctcggggc gggaagaacc tggtccggtc aggctgcggc cttggccgcc agttggccgc
    17641 aggcggcgtc tatgtcgcgg cctcgggtga ggcggacggt ggcgtggatt ccgcgtttca
    17701 tcagggtgtc gcggaagcgg tcgatcgctt cgcggggtga gccctggtag ccggttcccg
    17761 acgggttgta ggggatcagg ttgaccttga agtcgttctt cggaaggagc aggtcggcca
    17821 gttcgtgggc gagctcgacc gagtcgttta ccccgtcgag catcaggtac tcgataaaga
    17881 ccttgcgctt gcgggtgtgg cgccactcgc ggcaggcggc gaccacgtcc tccatcgggt
    17941 agcggtcgtt gacaggcatg atttcggagc ggagcttgtc gttcggggcg tgaagcgaga
    18001 gggcgaggcg gaccgccggg ccttccgtcg tcatgcgttc gatcccgggc tgccagccga
    18061 cggttgaaac cgtggtgtgg gagttggcga tcccgacttc cggcagcctt tcgcagacgg
    18121 ccagcacgtt gtcgaggttc atcatcggct cgcccatgcc catgaagacg gcgtggttga
    18181 ccggctcgat ccgccggaag tgaagggcct ggtcgaggat ctccgactcg gtcaggttgc
    18241 ggccgaattt catcgtgccg gtggcgcaga aggtgcaagt gagcgggcag cccgactggc
    18301 tggagaggca gagtgaacgg cggccgtcgc ggtagcgcat cagtaccgcc tcgaccgggc
    18361 ggccgtcggc ggtcagaaag agcgccttct cggtgccatc gaccgacttc atcgaggcgt
    18421 cgacttccag ggtcgagatc ggcagcctgc cttcgagttc ctgccgcagc gaggcgggca
    18481 ggttcgtcat ctcgtcgaat gaaccggccc cgcgggccag ccactcccag acctgaccgg
    18541 tccggtaggc cggctgctcc aggcccgcca tcgtttcttc aagaagcgtc agatccacac
    18601 caagagtgtg gcaggagctc acttcttctt ctttgagtcc ttcttcttcg gcttcttcgg
    18661 tgccttcgaa gcctttggat ccttcgggct cttctttgcc ggcttcttcg tcttctttga
    18721 cttcttgagc gttgcggcga cgagatcggt ctgtggtgcc cttcgcagtt ccttcttcgc
    18781 ggctttcacc ttgccggact ccggcgccgg ctctgcttca ggtgcgggct cgggttccgg
    18841 ttcgggttcc ggttccgggt cggggtcggg ctttggttct ggttccgagg ccggggatgg
    18901 tggagatcct tccgtgctca accgctccca gacgacggca agcaggaatg ccgcgccgat
    18961 caggatcgcc agcgccgcgc cccagaaggc cagctggaag atcagctcgc ccgggaaggc
    19021 gagggcacac atgaccagcc cgatgctgag gaccatgctg gtgatcgcga ccacgatcac
    19081 gtggacctgg aagcggttcc agtccggatc cgcgtcaccc gaggaagcgg gtcgcacgtt
    19141 gatcgccgtg gccacgaaca gaccggccag ggcagtcagg agcggcggga agaccgatga
    19201 atccgggttg atgccggtca ggatcaggcc cagtgcgagt accaggacgg cgtacgccag
    19261 ggtgccgccg acccacacgg caaccggccg gggtgctttc tcctcgtttt cctccatccc
    19321 tgccatcaac ttcgaagcca gtcgcggatt tcctccgagt gagcgttctc ggccgggggc
    19381 gggagtcgcg tttcggccgg ggtgcgcccc agccggatcg gtgaggcggg tgtgcgaacc
    19441 ccgtcgatct cgtcgatcgg gtcgagcccg agtgcctcgg ccaccgcgaa gccacggtcg
    19501 atcgtgttta ccggaccggc cgggaccctt gcctcatgca gggcggtcac ccactcttca
    19561 acggtccggc gggagaacag ttcgttcagt tcggcctcga gctcgacccg gtgctcgacc
    19621 cggtcgaagt tgtcgacata gcgcgggtcc gatgacagct caggctcgcc gagcacctcg
    19681 caaagcgcct tgaactggcg gtcgttgccg acgcagatca tcagcggacc gtcgctcgcg
    19741 tcgaaagtgg tgaacggctc gatgctcgga tggacgttgc ccagccggac cggcagcacc
    19801 ccggtgttga gccaggccga cgcctgattc tcgagcgccg ccagggccga actcagcagg
    19861 ttgctttcca ctttctggcc ctgccccgac ttcgcccttt cctgcagtgc gatgagcacc
    19921 ccgatcgtcg cgttgaggcc cgcgatcacg tcaaccagag caaccccgac cttgctcggg
    19981 tcaccttcgg gaggaccggt caccgacatc atcccgctca tcgcctggac cagcgggtcg
    20041 tagccgggca gggcggcccc ctccttcgag ccgaagccgc tgatcgagca gtagacgagc
    20101 ccggggttgg ccgcggccat ttcctcgtaa ccgagaccga gccggtccat cgtgccgggc
    20161 cggaagttct cgaccagcac gtcggcccgc ccgaccagtt cctttgccag ttccagatcc
    20221 tcggggtcct tgaggttgag ctcgaccgac cgcttgtttc ggttgacggc gagaaagaag
    20281 gtcgccctgc cctgggcgtc gaccggcggc gaccaggccc ttgtctcatc ccctccgggc
    20341 ggctcgacct tgatcacgtc ggcaccgaaa tcggccaggg tcatcgaaac gagcggaccg
    20401 gccagcaccc ggctgaagtc cgccaccaag agtccgtcca gagcattcac gccccgcata
    20461 atgccgtcca cagccgctcg cgtccagagg cgatcgccag gatcagcgtt ttcccgcagt
    20521 cattcttccc ggggtgcgtg gagccattcg cggatctcct cgccgtgctg atccaggtcg
    20581 ggtggcggaa accgcctctc cgggggactg cccgaaagac ggatcggcga cgaggtcgtg
    20641 cgggtccctt caatctcggt gaccgggtcg agctcgaggt tcccggccag cccgaaaccg
    20701 cccccgatgt cattgaccgg accggccggc accccggcgt cattcagcgc ttccgcccag
    20761 tactcgaccg aatcgagtcc gagccgccgc tccagctcct cccgcagggg ggcccggttc
    20821 tcaacccggt cgaggttggt cgcgaagcgg ggatcatcgg tcaggccggg ggcgccgtac
    20881 tggctgacga gcacgttggc cggcttgatg tcccggtgga cgatgccggc gcggtgggcg
    20941 gtctcgacgg cgctcgcgag ctggatgcca gtgcgcagga cctcctcgac ggt
//
LOCUS       DAOQKN010000018        20708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_1122_length_20708_cov_6.363095, whole genome shotgun sequence.
ACCESSION   DAOQKN010000018 DAOQKN010000000
VERSION     DAOQKN010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 20708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20708
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1122_length_20708_cov_6.363095"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="PLJ64_02370"
     CDS             complement(<1..859)
                     /locus_tag="PLJ64_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNC92370.1"
                     /translation="MAKDLYEVLGVGKKASQEEIKKAHRKLVRQYHPDRNPDDKAAED
                     KFKEIQAAYDILGDEEKRKQYDSGGGIFGGFGQGGGNPFGQSGAQGNPFGQGAPGGIG
                     DIFSTIFSRGGGGPAGPERGRDLETDARISFDDAMAGTQLTVTIPKSERCPTCSGSGA
                     EPGTSPETCPRCGGRGVEAQGQGFFSISQPCPQCQGTGQIIPSPCHTCGGSGLTHQTK
                     RYKVNIPAGVKDGTRIRVAGKGEAGVRGAPSGDLFVTIQVSPSPVFKRLEDGNLEVEV
                     PVSVTEAMRG"
     gene            complement(881..1522)
                     /locus_tag="PLJ64_02375"
     CDS             complement(881..1522)
                     /locus_tag="PLJ64_02375"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNC92371.1"
                     /translation="MIQEPEETTGQPEVEEVVDAEVVSAADPAVEDAVVVEDAIAEEA
                     AAEATVESDLAALVEEARSQRDEYLDLAQRTKADFDNYRKRVAAESQAQIERGRLEVV
                     SGLIEAIDNLERVLEAAGIEQEAALGGSLPEDAPVTRQGVIVAYRDLQSVLRKAGVEA
                     FDSKGEAFDPNLHEALQALPAEGVESGVVIEEMQKGYRAGESVIRHARVVVSQ"
     gene            complement(1519..3420)
                     /gene="dnaK"
                     /locus_tag="PLJ64_02380"
     CDS             complement(1519..3420)
                     /gene="dnaK"
                     /locus_tag="PLJ64_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006303662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNC92372.1"
                     /translation="MGKTIGIDLGTTNSCVAVLEGGEPTVLENSEGGRTTPSVVAFND
                     KDERLVGTVAKRQAVMNPENTIFSIKRFMGRKEAEVKEEETMIPYKVEAGPNGDVRVD
                     VRGKQYSPPEISAMILGKLKADAEAKLGETVDSAVITVPAYFNDDQRQATKDAGKIAG
                     LDVKRIINEPTAASLAYGLDKEDTDQTILVFDLGGGTFDVSVLEIGDGVFDVKSTAGD
                     NHLGGDNFDKAVVDWLVAEFKKDQGVDLAADKNSLQRLYEAAEKAKIELSSAQETQIN
                     IPFITAVEGQPKHLDIKLTRSKLNELTADLLDRVVGPVKQALSDADASVDHVVLVGGM
                     TRMPAVREKVTELTGKEPHQGVNPDEAVAVGAAIQAGVLAGDVKDVLLLDVTPLTLGI
                     ETKGGVMTKLIERNTTIPSRKSEVFSTAEDNQPSVEIHVLQGEREMATYNKSLGKFQL
                     TGIPPAPRGMPQIEVTFDIDANGIINVTAKDLGTGKEQKIEIKSGSGLSDDEINSMVS
                     DAEAHADEDKKQRELAEAKNIGENAAYTSEKQLTELGDKIDGASKTEIEDKIAALRGS
                     LDSEDAEDIKAKTEELQAAFHKVSEQVYQAAQAEQAAGAEGGADAGAASPDEEEVIDA
                     EVVDEDENK"
     gene            complement(3579..4706)
                     /locus_tag="PLJ64_02385"
     CDS             complement(3579..4706)
                     /locus_tag="PLJ64_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92373.1"
                     /translation="MFNTKNRVVFALLAIFTLSALLAGPAQAKRKHKPKKPNTVKVMS
                     RNLYLGADLGPAINSSDAADFISKNGQILRDVDTNNFPVRARGLAGEIRKVSPDLVGL
                     QEVALWRTGDASFTPSITGEYTASTVKVDFLADLLAQLNKGGVKRYRVVKVQNEFDFE
                     APADYDNDPATGAVTLGGEINGRLTMRDAVLAKVGAGIRTRNIKSGNYEHIFQPVISG
                     IPVKVKRGWLSADVKVRKSKWFRFVNTHFEAFGEPQIREQQAKELLAGPLKSGTKPAV
                     LVGDLNSDDDTVSEDDALAYDALTKGGMVDRGTQKMTFGVDSGIITDDAPTNFNQTID
                     HVMTNRPKSVKLLASGVTGTKPLNGYWDSDHRGVWSNLRIP"
     gene            complement(4909..5316)
                     /locus_tag="PLJ64_02390"
     CDS             complement(4909..5316)
                     /locus_tag="PLJ64_02390"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNC92374.1"
                     /translation="MNKKPFVIFGLLLILLAVVIPFWAFEKDGNTANNEGIPSESTPG
                     KQLFQTNCGNCHTLYAAGTDGDFGPNLDAKLAPAGPPADETAVKGIKTQVMAAIEEGA
                     DSPDVPGRMPAGIVTGTVADEIADFVAHYAGEG"
     gene            complement(5362..6138)
                     /gene="nth"
                     /locus_tag="PLJ64_02395"
     CDS             complement(5362..6138)
                     /gene="nth"
                     /locus_tag="PLJ64_02395"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNC92375.1"
                     /translation="MAAWRAKVAKKEWKRPTRARVRAIRDRLREMYGRPVNLPHRQPI
                     DELVRTILSQATSDANRDRAFAGLRDRFDDWEAVRDAPESEVEAAIRPGGLSRQKAPR
                     IQAVLREIEDLDLGWLEHADRALAMEFLTGLPGVGRKTAACVQVFTWNRPEIPVDVHV
                     FRIGGRLGLFRPNASFEEAHDELLALVDPADSYEFHMNLIRHGRTLCRPTPLCRECDL
                     RRMCPYGRGPGSERQPAGRTREGGPQSGVGTVGRGSGSEG"
     gene            complement(6142..7077)
                     /locus_tag="PLJ64_02400"
     CDS             complement(6142..7077)
                     /locus_tag="PLJ64_02400"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNC92376.1"
                     /translation="MERERPARPHSDQRPPIACLGEAIADLICERNLAKGEAPGPFVP
                     WPGGALANVAVAIARSGAPAALVGGVGGDGWGRWLADGLESEGVSTRWLATLDGADTP
                     VALIEFDSGNEPAFQVYGEHIGPTMAATGDFLEEAITGSRAVVIGSNTMVGEVERNVT
                     RRAVELAAAAGLPVLLDPNYRPNRWTSEEPARDFCRELSGSATVVKLNQGEAEMITGI
                     GEPLAAARDLLKLGPELVVVTNGEGEIVTAGAVEARHRPEPVETVSPLGAGDAFMGGL
                     AAGLAGLDWDLSRAAEVLPGAALAAAAVCGHWGAQ"
     gene            7147..7929
                     /locus_tag="PLJ64_02405"
     CDS             7147..7929
                     /locus_tag="PLJ64_02405"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNC92377.1"
                     /translation="MPALIVRIGEWLPRGWPDLFRQLLLYVLADTFYEMARGMADGRA
                     NVAFANGERVIDTERTLGMFFEPGVQSALHGFTWLIDIANTIYLNSQFTVALGFLIWM
                     YLFRNDYYYFFRNMLFVSMGIALVGYIGFPTAPPRMYPEYGFIDTVNAQSSVNHDSAL
                     AKLFINPFAAVPSMHCAVALMIGVTGFQVCRNWFAKTFWAGWPLVVAWVTVVTANHYW
                     IDAVLGWGVAGLSFLIASRVLAEIKPETWAWSREAERQRAEA"
     gene            7944..9554
                     /locus_tag="PLJ64_02410"
     CDS             7944..9554
                     /locus_tag="PLJ64_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92378.1"
                     /translation="MRLRSLTSPAAWGFLAAPLLVVVGLAVVYLIMAPHSADHAAQTF
                     RTELFELSGPTVWNNYWFAGHYLPSYSLLSPPLGAWLGFRVMGSLAVIGTVILFTLIV
                     RREWGERAQAGAIWFAFAATISLFSGRLTFALGCLLAMAAVFASQRGLRIPALLLAGS
                     VGLASPVAALFLACLGFAHFLAERPDRRGLELAGVSFLVAAIVALLFPGGGDEPYVTS
                     SFIPAIGLTLVSAWMVPPGQRLVRTGMLVYAGALVASYVLSTPMGGNVNRLGALLLGP
                     VLLCVLAAEPLTPKLWRRGVLIILLPMIVWWQTGPVIRDLKLTDDDPAVTQAFYQPLA
                     DALEPRLVREPARVEVVPIASHWEAARAAPEFPLARGWERQTDRNFNPLFYADRLGPN
                     RYRQWLDRLAVGYVALPADTELDYAGEKEAALIRSGLPFLREIPVGGDWRLFKVIGAQ
                     PMVARPARLTELDTDGFAVAAPIAGSFEVRIRSTPYWKVKSGFGCVEPGRGDWTRVTL
                     DRPGSLKVAADFSPGARFGSDRDCRPDR"
     gene            9585..10235
                     /locus_tag="PLJ64_02415"
     CDS             9585..10235
                     /locus_tag="PLJ64_02415"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNC92379.1"
                     /translation="MESSSGPGGGQNGSRRPAQEFREQARNALIQSRLTPNAISMMGL
                     VGNLIAAGLVLHGDFFFAGIAFIIGSVMDTLDGRYSRMSGKGTLFGAFLDSTLDRIEE
                     GIVLAAVAWQFADSGDSVAAAMCVVVVLGSLMVSYTRARAEALGVECKVGIATRPVRV
                     VILSAGLVLGGEEVISGFDLLPIAIYVMAGLTAITTLQRVWHVRTELKKLDAAATE"
     gene            10416..11609
                     /locus_tag="PLJ64_02420"
     CDS             10416..11609
                     /locus_tag="PLJ64_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HNC92380.1"
                     /translation="MTENGLNGNGNGASRGGDDKVRVAIVGVGNCANSFIQGVQYYKD
                     ADPADQVPGLMHVDLGGYHVKDIEFVAAFDIDQEKVGKDLSEAIWSGQNDTIKFADVP
                     NLGVTVERGMTLDGLGKYLKQVITKAPGPTADIVQILKDTKTDVLVCYLPVGSEDAVK
                     WYVEQAIAAGVGFVNCLPVFIASQDYWDGRFKEAGLPIIGDDIKSQVGATIVHRQLAR
                     LFHERGVEMQHTSQLNVGGNMDFFNMLERERLDSKKISKTNAVTSIMGTELEDRDVHV
                     GPSDYVPWLTDRKWAHIRLEGKSFGDVPLNVELKLEVWDSPNSAGVVIDAVRLIKLAL
                     NNGIAGQLDGPSSYLMKSPHNQRPDAEARQQTEDFIVENARGKSALPGTKPVAESAED
                     AKSEA"
     gene            11611..13575
                     /locus_tag="PLJ64_02425"
     CDS             11611..13575
                     /locus_tag="PLJ64_02425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC92381.1"
                     /translation="MTTGSALSIVQFTPFPWSERGGINEYTRRVSEELARRGHSVLIA
                     APSGGRGGVTETRAALAELADDPDALYRPGEEPRVLSMGGGVKIPRTGKRQAPVSVDV
                     GNRVEALLGTGRFDIVHVHDPFVPGFSSTALRHSFSLNVGTFHETEERPFATQFARPL
                     LEIFFGRLDARTASSKTSAKLLNRYFPGSYEITHPGVDPAEPARVEVPPLKIAFSERE
                     EPGALRLFLRALRRLPADLDWTASIYADDPGEVQLRVASAIRDRIKVIGPEQVSLADL
                     LSHSDVFVAASNGPRPDPAAVRQAIASGAVPVTTTIGRYVELVGDGQRGLLFPAGDPV
                     TLAGQIQRLGEDSRLREGFRAQCGKFEVESWSGVAGRFEAIYERLVSRRHDPAGNEEL
                     RKKLRDRPLIEVDLHMHTDHSSDCATPVKVLIETARDRGFGAIAITDHNEVSGAMEAA
                     EVAAEMDDFKIIVAEEVMTGDAGEVIGLFLKEKIPKGTSMSDTIREIKRQGGLVYVPH
                     PFDRLHSVPDYENLLDIVEDIDLMEVFNPRVAISVFNEEAERFARKYNIIPAAGSDSH
                     VAQGLGAVRNRLHDFDGPEEFLEAMREAEITRRHKNLVYVQALKFIQTTGRPKAAKRQ
                     VDDPQAVRGGRRKARGSKQARGPKRAAGKS"
     gene            13644..14249
                     /locus_tag="PLJ64_02430"
     CDS             13644..14249
                     /locus_tag="PLJ64_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HNC92382.1"
                     /translation="MPATENEIKEKYLERAIRELNQLNRDLQGCDACLTTKVMPVLGS
                     GHPQADVMLVKFAPTTAEVEEGVAFYGRAGNALMKSFKRLNIDPTVIYGTVCAKCPVE
                     DPTRADPECVARLADEMGIVQPRIVVVMGEPALETINSLALPLARPLEPREGEIQAFS
                     PACDALFTPDIDGALDEETAKRRFWRAFRTLGRWHDDLPPY"
     gene            14401..14796
                     /locus_tag="PLJ64_02435"
     CDS             14401..14796
                     /locus_tag="PLJ64_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92383.1"
                     /translation="MGTVEQTVIGPREGHANGKALAAMILGVFAIANVVVLALFMALS
                     AETLDDSGAWWMLLPFFGLTAGLIAAFLGSLGWIDVRRGITDRRLFEAQLGAILGGVA
                     AGLILLAITVLLVMALFLAFSFAGEGGVD"
     gene            complement(14817..15542)
                     /locus_tag="PLJ64_02440"
     CDS             complement(14817..15542)
                     /locus_tag="PLJ64_02440"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNC92384.1"
                     /translation="MPDREKISRYAKATAKAGKGGELADLLLEAAEVNRASEGCLQYE
                     VHQSVFDPDTIWVTEAWVDQDAVKASLEDEENKTLIDRARPLIEDMEVIELLPLGGIE
                     ARLDDPRAATPKPGYKLVNVAEVNDGAKDHGLGEIQEARFAIGDLEASQVGVAWFRMH
                     PNQRQPFGHVHDRAEEVYFVVSGSGRVKLDDDIVELREGDLIRVAPEIIRCFEAGPDG
                     MVYYAAGQHMSGDGDVKPGWWSD"
     gene            complement(15587..16192)
                     /locus_tag="PLJ64_02445"
     CDS             complement(15587..16192)
                     /locus_tag="PLJ64_02445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HNC92385.1"
                     /translation="MSAPPLDPAPSPSPPSDERPGMDVTRPRTPAGMIGWSIFASRWI
                     QAPLYLGLIVAQAVYVVKFWKELVHLITDFSSFDENAVMLTVLGLVDVVMIANLLIMV
                     IIGGYETFVSRIKVENHPDQPEWLSHVNANVLKVKLAMAIVGISSIHLLKTFIEYGTE
                     ETGLTSTEVMWQTIIHFTFVLSAIGLAYIDRYLSPTKDDGL"
     gene            complement(16257..17051)
                     /locus_tag="PLJ64_02450"
     CDS             complement(16257..17051)
                     /locus_tag="PLJ64_02450"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNC92386.1"
                     /translation="MTRPEQKRAFGVAAGLPAEVCAPLAAACEEAGYASVWANDHPLA
                     KGLETLAEFAKGADGVELGVAVIALDRQSPAEIAADVERLGLDPSRLWIGVGAGFTKK
                     PLTFMTEQIGELREKLPGIRVIMAAMGPKMCALAGSEYDGAFFNWMTPEFAAGARTKV
                     EAGAADAGHEAPPVFGYVRTAVGPDAAERLAKEESFYRDLHDGYRNHFARLGEPEGTV
                     GVAAADSAEAQEKLAAYEALDVIVVRGLASAKVEPMVELARAAAPA"
     gene            complement(17048..18481)
                     /gene="rlmD"
                     /locus_tag="PLJ64_02455"
     CDS             complement(17048..18481)
                     /gene="rlmD"
                     /locus_tag="PLJ64_02455"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HNC92387.1"
                     /translation="MSATQPSSPPTRLRRGETFEGEIETLAYGGRGITKPDGYVVFVN
                     GGLPGDRVRALITKSKRGYAEANALEIITPSPDRLPDTDLHQGEACPGASWQALSYEK
                     QLAFKEEHVADALARIGKLGDVNIDPIVEADEQWRYRNKVEYSFGEQDGELVLGFHPR
                     GRWDEVLDIEDCHLSSEATNRARNEVLAWARAEGLNAYDQHDHRGILRNLVVREGRRT
                     GQVQTRLVTSRANFPKPPVDLHTIIDGPSGSTSGPTGVLGEEVLRERIFDLDIELSHD
                     AFFQTNTEMAEKLYGLAAEYAGLTGNEKLFDLFCGVGTIGLSMARQAGQVWGLEIVPE
                     AIENAKRNAKANGITNARFVAANARTGMRPLIEEAGRPDVVTIDPPRAGLSAKIVRRL
                     IECEARRIVYISCNPTTLAPNAAQLVEAGYRLTRVRPVDMFPQTPHIECVAQFDLVGE
                     VKYVESPTDRKRRERDERRQAAKEAEG"
     gene            18529..19422
                     /gene="argF"
                     /locus_tag="PLJ64_02460"
     CDS             18529..19422
                     /gene="argF"
                     /locus_tag="PLJ64_02460"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNC92388.1"
                     /translation="MTEPRARQYIDGHEISGGHLVNLIDRAEELRAGRPNEGGSALAG
                     KSVAMLFEKPSTRTRISFEVGTVELGGHPVVLRGSEMQLSRGESVEDTARVLSRFVSA
                     IVIRSGSHENVVALAENASVPVINALTPEFHPCQALADLQTLKQRFGTLNGLKVAYVG
                     DGNNVARSLAIAGSLTGVEVVVAAPAGYQLETEVPAKLTDDPIEAVHRADAIYGDVWV
                     SMGDEASAGQRRADLAPYRIDGHLLSHARQETIVMHCLPAHPGEEITADVLYGDRSAV
                     WDQAENRLHAQKALLEYLLAP"
     gene            19619..19690
                     /locus_tag="PLJ64_02465"
     tRNA            19619..19690
                     /locus_tag="PLJ64_02465"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19652..19654,aa:Glu,seq:ttc)
     gene            19769..20563
                     /locus_tag="PLJ64_02470"
     CDS             19769..20563
                     /locus_tag="PLJ64_02470"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNC92389.1"
                     /translation="MIPEISLGPITLQTFGLAFAAAFIVSGWIVHLRLEEIGKPPDWA
                     YEMVFAALIGGLIGARAYFIFDNYADVKDDLLGNIFSGSGLTWYGGAIGGAIGVILWA
                     WWRKFLNLWLLDTAAPALAAGYAVGRIGCQLSGDGDYGKPWDGPWAMSYPDGTVPTPD
                     GVTVHPTPIYETLTMGLGAYILWRLRDRFRLGILFAIYLIYAGTERLLVEFLRRNTDV
                     FLGLTQAQLESVAMILGGIIWILVVRRRYGSLARPADQMSAADVPA"
     gene            20634..20706
                     /locus_tag="PLJ64_02475"
     tRNA            20634..20706
                     /locus_tag="PLJ64_02475"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20667..20669,aa:Val,seq:tac)
BASE COUNT         3159 a         7017 c         6755 g         3777 t
ORIGIN      
        1 cgccccgcat cgcctcggtg accgagaccg gaacctcgac ctcgaggttg ccgtcctcga
       61 gtcgcttgaa gacgggggag ggtgaaacct ggatcgtcac gaagaggtcg cccgacgggg
      121 cgccgcggac tcccgcctcg cccttgccgg cgacccggat ccgggtaccg tccttgaccc
      181 ccgccgggat gttcaccttg tagcgcttgg tctggtgggt cagtccggaa ccgccgcagg
      241 tatggcaggg gctggggatg atctgacccg tgccctggca ctgcgggcag ggctggctga
      301 tcgagaagaa accctggccc tgggcctcga ctccgcggcc gccacagcgg gggcaggtct
      361 ccgggctggt gcccggctcg gccccggaac ccgagcaggt cgggcagcgt tccgacttcg
      421 ggatcgtgac cgtgagctgg gtaccggcca tcgcatcgtc gaaggagatg cgggcgtcgg
      481 tttcaagatc gcgaccgcgt tccggcccgg cggggccgcc cccgccgcgg ctgaaaatgg
      541 tcgagaagat gtcaccgatc ccgccgggag ctccctggcc gaagggattg ccctgggcgc
      601 cgctctggcc gaacgggttg ccgccgccct ggccgaagcc gccgaagatc ccgccgccgg
      661 agtcgtactg ctttcgcttc tcctcgtcac cgaggatgtc gtaggccgcc tggatctcct
      721 tgaacttgtc ctcggccgcc ttgtcatccg gattgcggtc cgggtgatac tgccgcacca
      781 gcttgcggtg ggctttcttt atctcttcct gtgaagcctt cttgcctacg ccgaggactt
      841 cgtaaaggtc tttcgccata cggcgttcac tttctcttca tcactggctg accactacgc
      901 gggcgtgtct gatcacactt tccccggcgc ggtagccctt ctgcatctct tcgattacga
      961 cgcctgactc gacgccttcg gccggcagcg cctgcagcgc ctcgtggagg ttggggtcga
     1021 atgcctcgcc cttcgagtcg aaggcttcga ccccggcctt gcgcaggacc gattgaaggt
     1081 cgcggtaggc gacgatcacg ccctgccggg tgaccggggc gtcctccggc agggatccgc
     1141 cgagtgcggc ttcctgctcg attccggccg cttcgagtac ccgctcgagg ttgtcgatcg
     1201 cctcgatcag gccggagacg acttccagcc ggccccgttc gatctgggcc tggctttcgg
     1261 ccgcgacgcg cttgcggtag ttgtcgaagt cggccttggt ccgctgggcc aggtcgaggt
     1321 attcgtcccg ctggctgcgg gcctcctcga ccagcgccgc caggtcgctt tccacggtcg
     1381 cttcggcggc cgcctcttcg gcgatcgcgt cttcaacgac cacggcgtcc tcgacagccg
     1441 gatcggccgc cgacaccact tcggcgtcga cgacctcttc cacctcgggc tggcccgtgg
     1501 tctcttccgg ctcctggatc acttgttctc gtcctcgtca accacttcgg cgtcgatcac
     1561 ttcctcttca tccggggaag cggctccggc atcagcgcca ccttcggcgc ccgcagcctg
     1621 ctcggcctgg gcggcctggt agacctgctc ggacaccttg tggaaggcgg cctggagctc
     1681 ttcggtcttc gccttgatgt cctcggcgtc ctccgaatca agcgatcccc gcagggccgc
     1741 gatcttgtct tcgatctcgg tcttcgaggc accgtcgatc ttgtcgccga gctcagtcag
     1801 ctgcttctcc gaggtatagg cggcgttctc gccgatgttc ttggcttcgg ccagctcgcg
     1861 ctgcttcttg tcctcgtcgg catgcgcttc ggcgtcggag accatgctgt tgatctcgtc
     1921 gtccgagagt ccggaaccgg acttgatctc gatcttctgc tccttgccgg tgccgagatc
     1981 cttcgcggtc acgttgatga tgccgttggc gtcgatgtcg aaggtgacct cgatctgcgg
     2041 catgccacgc ggagccggcg ggatgccggt cagctggaac ttgccgaggc tcttgttgta
     2101 ggtagccatc tcgcgctcgc cctggaggac gtggatctcg accgagggct ggttgtcctc
     2161 ggcggtcgag aagacctccg acttgcggga cggaatggtc gtgttgcgct cgatcagctt
     2221 ggtcatcacg ccgcccttgg tttcgatacc gagggtgagc ggggtgacgt cgagcagcag
     2281 cacgtccttg acgtcaccgg ccagcacgcc ggcctggatc gcggcaccga ccgccacggc
     2341 ctcatccggg ttgacaccct gatgcggctc cttgccggtc agctcggtga ccttctcgcg
     2401 gaccgcgggc atacgggtca tgccgccgac caggaccacg tggtccacgc tggcgtcggc
     2461 gtcggagagc gcctgcttga ccggcccgac cacgcggtcg agcaggtcgg cggtcagctc
     2521 gttcagcttg gagcgggtca gcttgatgtc gaggtgcttg ggctggcctt caaccgcggt
     2581 gatgaagggg atgttgatct gcgtctcctg ggcggaggac agctcgatct tcgccttctc
     2641 cgccgcctcg tagaggcgct gcagcgagtt cttgtcggcg gccaggtcga ctccctggtc
     2701 cttcttgaac tcggcgacca gccagtccac gactgccttg tcgaagttgt cgccgccgag
     2761 gtggttgtca ccggcggttg acttcacgtc gaagacgccg tcaccgatct cgagcacgga
     2821 cacgtcgaag gtgccgccgc cgaggtcgaa gaccaggatc gtctggtcgg tgtcttcctt
     2881 gtcgaggccg taggccagcg aggccgcggt cggctcgttg atgatgcgct tgacgtcgag
     2941 tccggcgatc ttgccggcgt cctttgtcgc ctggcgctga tcgtcgttga agtaggccgg
     3001 gacggtgatc acggccgagt cgaccgtctc gccgagcttg gcttcggcgt ccgccttcag
     3061 cttgccgagg atcatcgcgc tgatctcggg cggcgagtac tgcttgccgc gcacgtccac
     3121 gcggacatcc ccgttcgggc cggcttcgac cttgtacggg atcatcgtct cctcttcctt
     3181 gacctcggcc tccttgcggc ccatgaagcg cttgatcgag aagatcgtgt tttccgggtt
     3241 catcacggcc tggcgcttgg ctacggtgcc gaccagccgc tcgtccttgt cattgaaggc
     3301 gaccaccgac ggggtggtgc gcccgccctc ggagttctcg agaacggtcg gttcgccgcc
     3361 ttcgagaacc gctacgcacg agttcgtcgt gccgaggtcg attccaatcg tctttcccac
     3421 tgtgtttctc ctgtttggat gtgtcagaga ccgcatgagg gtgcggtcat ggtcaaaaat
     3481 ctaagtggga ctgtataaga ttttgtacag aacgtcaaat taacgaagaa aggcccgcaa
     3541 atgcgggcct ttccggcaat tcggtttgtc tcgttgcgct acggaatgcg caggttcgac
     3601 cagactccgc ggtggtcgga atcccagtag ccgttgagcg gcttggttcc ggtcaccccg
     3661 gaggcgagca gcttgaccga cttcggacga ttggtcatca cgtgatcgat cgtctggttg
     3721 aagttggtcg gggcatcgtc ggtgatgatg ccgctgtcca cgccgaaggt catcttctgc
     3781 gtgccgcgat cgaccatgcc gcccttggtc agggcgtcgt aggcgagcgc gtcatcctcg
     3841 gagacggtgt cgtcatccga attcaggtca ccgaccagaa ccgcgggctt tgtaccgctc
     3901 ttgagcggtc cggccagcag ctctttggcc tgctgctcgc ggatctgcgg ttcgccgaag
     3961 gcctcgaagt gcgtatttac gaagcggaac cacttgctct tgcggacctt cacgtcagcg
     4021 gaaagccagc cgcgcttcac cttgaccggg attccggaga tgaccggctg gaagatgtgc
     4081 tcgtagttgc cggacttgat gttgcgggtg cggatgccgg caccgacctt ggccaggacc
     4141 gcgtcacgca tggtcaggcg accgttgatc tcgcccccga gagtgactgc gccggtggcg
     4201 ggatcattgt cgtagtcggc gggtgcctcg aagtcgaatt cgttctggac cttgaccacc
     4261 cggtagcgct tgactccacc cttgttgagc tgcgcgagca ggtcggcgag gaagtccacc
     4321 ttgacggtag aggccgtgta ctcaccggtg atcgagggcg tgaagctggc gtcgccggtg
     4381 cgccagagag cgacttcctg aaggccgacg aggtccgggc ttaccttcct gatttcgccg
     4441 gcgaggcccc tggcccggac cgggaagttg ttggtgtcca cgtccctcag gatctggccg
     4501 ttcttgctga tgaagtcggc ggcgtcggac gagttgatcg ccgggccgag gtcggctccc
     4561 agatacaggt tgcgggacat caccttgacg gtgttcggct tcttcggctt gtgcttgcgc
     4621 ttggcctggg ccgggccggc cagcagcgcg gaaagcgtga agatcgccag cagggcgaag
     4681 accacgcggt tcttggtgtt gaacatcaag ttctctcctt ttggtctggg gcgaggcgcg
     4741 gcaacgcgcc ttctgcggtt ccccaaccgc tgtcaggagg ataaacccgg caagggcccg
     4801 aacgatgcgg agactgatca atcggagccg gcccgaccat cagcgaatca agcgggccgg
     4861 cgggttcccg ttgtcatccg gcgaagaccg gatgacgcct tgttgtccct aaccttcgcc
     4921 ggcgtaatgg gcgacgaagt cggcaatctc gtcggcgacc gtgccggtga cgatgccggc
     4981 gggcatgcgg cctggaacgt cggggctgtc ggcgccttct tcgatggccg ccatgacctg
     5041 ggtcttgatt cccttgactg cggtctcgtc ggcgggcggg ccggccggag cgagcttggc
     5101 gtcgaggttc ggaccgaagt caccgtcggt gccggccgcg tagagcgtgt ggcagttgcc
     5161 gcagttggtc tggaagagct gcttgcccgg cgtgctctcg gagggaatcc cctcgttgtt
     5221 ggcggtgttg ccgtccttct cgaatgccca gaacgggatc accactgcca gcaggatcag
     5281 gaggagacca aatatgacga agggcttctt gttcatggac gggcggcgca aaagtggacg
     5341 gtgcgccgaa cgccgggaat attacccttc ggaccccgat ccgcgtccta ccgttccgac
     5401 cccgctctgc gggccgcctt cccgggtgcg ccccgcgggc tgccgttccg accccggtcc
     5461 gcggccgtag ggacacatcc ggcgcaggtc gcactcgcgg cagagcggcg tcggtcggca
     5521 aagagtgcgc ccgtgacgga tcaggttcat gtggaattcg tatgaatcgg ccgggtcgac
     5581 cagcgccagc agttcatcgt gggcttcctc gaaggaagcg ttcggccgga acagccccag
     5641 ccttccgccg atccgaaaca catgaacgtc gaccgggatc tccggccggt tccaggtgaa
     5701 gacctgaacg caggccgccg tcttgcgccc taccccgggc agtccggtca ggaactccat
     5761 cgcaagggcg cggtcggcgt gttcgagcca gccgaggtcg aggtcctcga tctccctgag
     5821 gacggcctgg atccgcggcg ccttctgacg gctgaggccc ccgggccgga tcgccgcctc
     5881 gacctcggac tcgggggcgt cgcgaaccgc ctcccagtca tcaaagcggt ccctcagacc
     5941 ggcaaaggcc cggtcgcgat tggcgtcact ggtcgcctgg gagaggatcg tccggaccag
     6001 ctcgtcgatc ggctggcggt gcggcaggtt gaccgggcgc ccgtacatct cccgcagccg
     6061 gtcgcggatc gcccgcaccc gggcccgggt gggccgtttc cattccttct tcgcgacttt
     6121 cgcccgccag gccgccaccg gttactgggc tccccagtgc ccgcagaccg cggccgccgc
     6181 gagggccgcc ccggggagca cctcggcggc gcgggaaagg tcccagtcga gcccggcgag
     6241 accggccgca aggccgccca tgaaagcgtc acccgctccg agcggactta ccgtttcgac
     6301 cggctcggga cgatgccgcg cctcgaccgc accggcggtg acgatttccc cctcaccgtt
     6361 ggtgaccacg accagctccg ggccgagctt gaggagatcc cgggccgcgg cgagcggctc
     6421 cccgatcccg gtgatcattt ccgcttcccc ctggttcagc ttgaccaccg ttgccgaccc
     6481 gctcaattcc cggcagaaat cgcgggccgg ttcttcgctg gtccagcggt tgggccggta
     6541 gttcgggtcg agcagcaccg gcagcccggc cgcggcagcc agctcgaccg cgcgccgggt
     6601 cacattccgc tcgacttcgc cgaccatcgt gttcgaaccg atgaccaccg cccgggaacc
     6661 ggtgatcgcc tcttcaagga agtcgccggt ggcggccatg gtcggcccga tgtgttcgcc
     6721 gtagacctgg aaagcgggct cgtttccgga atcgaactcg atcagggcga ccggagtgtc
     6781 tgccccgtcg agtgtggcca gccatctggt tgagacgcct tcggattcca gcccgtcggc
     6841 gagccagcgc ccccagccgt caccgccaac cccgccgacc agggcggccg gggcgccgct
     6901 tcgggcgatc gccacggcta cgttagccag cgcccctccc ggccacggaa cgaagggccc
     6961 gggtgcttcc cccttggcca ggttccgctc gcagatcaga tccgcgatcg cctcgccgag
     7021 acaggcgatc gggggtcgct gatcactgtg gggtcgggct ggccgttctc gctccatgac
     7081 tggaaagccg gagcctaccc gaggcttcct gagagaaatc tgcgcgcgcc attagactga
     7141 gcagtcatgc ccgcgctgat cgtccggatc ggggagtggc tcccccgcgg ctggcctgac
     7201 ctctttcgcc agctcctgct ctatgtgctc gccgacacct tctacgagat ggcgcgcgga
     7261 atggccgatg gccgggccaa tgtcgccttc gccaacggcg aacgggtgat cgacaccgaa
     7321 cgcaccctcg ggatgttctt cgaacctggt gtgcagagcg cccttcacgg cttcacctgg
     7381 ctgatcgaca tcgccaacac gatctacctc aacagccagt tcacggtggc gctcggcttc
     7441 ctgatctgga tgtacctgtt ccgcaacgac tactactact tcttccgcaa catgctcttc
     7501 gtctcgatgg gcatcgcact ggtgggctac atcggcttcc cgaccgcccc gccgcggatg
     7561 taccccgagt acggattcat tgacacggtc aacgcccagt ccagcgtcaa ccacgactcc
     7621 gccctggcca agctcttcat caaccccttc gccgcggtgc cttcgatgca ttgcgcggtc
     7681 gccctgatga tcggcgtcac cggtttccag gtctgcagga actggttcgc caagaccttc
     7741 tgggcgggct ggccgctggt ggtcgcctgg gtcaccgtgg tcaccgcgaa ccactactgg
     7801 atcgatgccg tgctcggctg gggcgtcgcc gggctctcct tcctgatcgc ttcgagagtg
     7861 ctggccgaga tcaaacccga gacctgggcc tggagccgcg aggcggagcg ccaacgggcc
     7921 gaggcctgag cggaggcccg gcggtgcgac tccgttccct gaccagcccg gccgcgtggg
     7981 gattcctggc cgcgccgctg ctcgtcgtgg tcgggctcgc ggtggtctac ctgatcatgg
     8041 cgccccacag cgccgatcac gccgcccaga ccttccgcac cgagctgttc gaactctccg
     8101 ggccgacggt ctggaacaac tactggttcg ccggccacta cctgccgtcc tattccctgc
     8161 tttcgccgcc gctcggggcc tggctcggct tccgggtgat gggttcactg gcggtgatcg
     8221 gaacggtgat cctcttcacc ctgatcgtcc ggcgcgagtg gggcgagcgg gcccaggccg
     8281 gggcgatctg gttcgccttc gcggccacga tcagcctgtt ttcggggcgg ctcaccttcg
     8341 ccctcggttg cctgctggcc atggcggcgg tcttcgcctc gcagcgcggc ctgcgtatcc
     8401 cggcgctgct cctggccggc tcggtcggtc tggccagccc ggtcgccgcc cttttcctgg
     8461 cctgtctcgg cttcgcgcat ttcctggccg agcgacccga ccgccgcggt ctggagctgg
     8521 ccggagtctc ctttctggtt gccgcgatcg tcgcgctgct ctttcccggc ggaggcgacg
     8581 agccgtatgt cacttcgagc ttcataccgg cgatcggcct gaccctggtc agcgcctgga
     8641 tggtccctcc gggccagcgc ctggtccgca ccgggatgct cgtctacgcc ggcgccctgg
     8701 tcgcctccta tgtgctcagc acgccgatgg gaggcaacgt caaccggctc ggggccctgc
     8761 tgctcgggcc ggtcctgctc tgcgtgctgg cggccgagcc gctgaccccg aaactgtggc
     8821 gccgcggcgt gctgatcatc ctgctgccga tgatcgtctg gtggcagacc gggccggtga
     8881 tccgggatct caagctgacc gatgacgatc cggcggtgac ccaggccttc taccagcctc
     8941 tggccgacgc cctcgagcca cgcctcgtac gcgagccggc cagggtcgag gtggtgccga
     9001 tcgcctccca ttgggaagct gcccgggcgg ccccggaatt cccgctcgcg cgcggctggg
     9061 agcggcagac cgaccgcaac ttcaacccgc tcttctatgc cgaccggctc ggcccgaacc
     9121 ggtaccgaca gtggctcgac cggctcgcgg tcgggtatgt ggccctgccg gccgacaccg
     9181 aactcgacta cgccggggag aaggaggcgg ccctgatccg ctccgggctt ccgttcctca
     9241 gggagatccc ggtcggcggc gactggcgac tgttcaaggt gatcggcgcg cagccgatgg
     9301 tcgcccgtcc ggcccgcctg accgaacttg acaccgacgg cttcgcggtc gcggcgccga
     9361 tcgccggcag cttcgaggtg agaatcaggt cgacccccta ctggaaggtc aagagcggat
     9421 ttggttgtgt cgagccgggc cggggtgact ggacccgggt cacgctcgat cgccccggat
     9481 cgctcaaggt cgccgccgac ttcagccccg gggcccggtt cggctccgac cgggactgtc
     9541 gccccgaccg gtgagccacg gctccaacct ctagagttcc ccgcatggag tcttcgtccg
     9601 gcccgggagg cggccagaac ggttcgcgca ggccagccca ggaattccgg gagcaggccc
     9661 gcaacgctct catccagtcc cggctgaccc cgaacgcgat ttcgatgatg gggctggtcg
     9721 gaaacctgat cgccgccggt ctcgtgcttc acggcgattt cttcttcgcc ggcatcgctt
     9781 tcatcatcgg ttcggtgatg gacaccctcg acggccgtta ctcgcggatg tcgggcaagg
     9841 gcacgctgtt cggcgccttc ctcgattcca ccctcgaccg gatcgaagag ggcatcgtgc
     9901 tcgccgcggt cgcctggcag ttcgccgaca gtggtgacag cgtggccgcc gcgatgtgcg
     9961 tggtggtcgt gctcggatcg ctgatggtca gctacacccg ggcccgagcc gaggcgctcg
    10021 gagtcgaatg caaggtcggt atcgccaccc ggccggttcg cgtcgtgatc ctttcggccg
    10081 ggctggtcct cggcggggag gaagtgatct cgggctttga cctgctgccg atcgccattt
    10141 acgtgatggc gggactgacc gcgatcacca ccctccagcg ggtctggcat gtccggaccg
    10201 agctgaagaa gctcgacgcg gccgcgactg aataggcgga caggcccgaa attaatgccc
    10261 ggcagcctgt aaccggggcc gttttgtggt gtcattaggg gtctacccgg ccccgcgccc
    10321 ggatcaggcg tggctttttg cccctgatca gcgtgcggac cggtgcccaa ccggcaagag
    10381 atgccacccc acccacagga gactttcgct tacccatgac tgagaacgga cttaacggaa
    10441 acggcaacgg cgccagccgt ggcggtgacg acaaggtacg agttgccatt gtcggcgtcg
    10501 gcaactgcgc caactccttc atccagggag tccagtacta caaggacgcc gatccggcgg
    10561 atcaggttcc cggacttatg catgtcgacc tcggcggcta ccacgtcaag gacatcgagt
    10621 tcgtggctgc cttcgacatc gatcaggaga aggtcggcaa ggacctttcc gaagcgatct
    10681 ggtcgggcca gaacgacacg atcaagttcg ccgacgttcc caacctcggc gtcacggtcg
    10741 agcgcggcat gaccctcgac ggcctcggca agtacctcaa gcaggtcatc accaaggccc
    10801 ccggcccgac cgccgacatc gtccagatcc tcaaggacac caagaccgac gttctggtct
    10861 gctacctgcc ggtcggctcc gaagacgccg tcaagtggta cgtcgagcag gcgatcgccg
    10921 ccggcgtcgg cttcgtcaac tgcctgccgg tcttcatcgc cagccaggat tactgggacg
    10981 gccgcttcaa ggaagccggc ctgccgatca tcggtgacga catcaagtcc caggtcggcg
    11041 cgaccatcgt ccaccgccag ctcgcccgcc tcttccacga gcgcggcgtc gagatgcagc
    11101 acacctcgca gctcaacgtc ggcggcaaca tggacttctt caacatgctc gagcgcgagc
    11161 gcctcgactc gaagaagatc tcgaagacca acgccgtcac ctcgatcatg ggcaccgagc
    11221 tcgaggaccg cgacgtccac gtcggcccct cggactacgt gccgtggctg accgaccgca
    11281 agtgggccca catccgcctc gagggcaagt ccttcggcga cgtaccgctc aacgtcgagc
    11341 tgaagctcga ggtctgggac tcgccgaact cggccggggt cgtgatcgac gccgtgcgcc
    11401 tgatcaagct cgccctgaac aacggcatcg ccgggcagct cgatggcccc tcctcgtacc
    11461 tcatgaagtc cccgcacaat cagcggccgg acgccgaggc ccgtcagcag acggaggact
    11521 tcatcgtcga gaacgctcgc ggcaagtcgg ctctcccggg caccaagccg gtcgccgaat
    11581 cggccgagga cgcgaagtcc gaagcctgat ttgaccacgg gttctgccct ttcgatagtt
    11641 cagttcaccc cgttcccctg gagcgagcgg ggtggcatca acgagtacac gcgccgggtt
    11701 agcgaagagc tggcccggcg cggtcattcg gtgctgatcg ctgccccttc cgggggccgc
    11761 ggcggcgtca ccgagaccag ggcggcactg gccgagctcg ccgacgaccc cgacgccctc
    11821 taccgccccg gggaggaacc cagggtcctt tcgatggggg gcggggtgaa gatcccgcgc
    11881 accgggaagc gtcaggcccc ggtttcggtt gacgtgggca accgggtcga agccctgctc
    11941 gggaccggtc gctttgacat cgtccacgtc cacgacccct tcgtgcccgg cttctcctcg
    12001 accgccctgc gccattcctt ctcgctcaac gtcggcacct ttcacgagac cgaggagcgg
    12061 cccttcgcga cccagttcgc ccggcccctg ctcgagatct ttttcggccg cctcgatgcg
    12121 aggaccgcat cctcgaagac ctcggccaag ctgctcaacc gttacttccc cggttcatac
    12181 gagatcaccc accccggggt cgacccggcc gagccggccc gggtcgaggt gccaccgctc
    12241 aagatcgcct tctccgagcg tgaggaaccg ggggcgctcc gcctcttcct gcgggccctg
    12301 cggcgcctgc cggccgacct cgactggacc gccagcatct acgcggacga tcccggcgaa
    12361 gtccagcttc gtgtggccag cgccattcgc gaccggatca aggtgatcgg tcccgaacag
    12421 gtttcgctcg cagacctgct ctcccattcc gatgtcttcg tggctgcctc caacggtccg
    12481 cggccggacc cggcggccgt tcgccaggcg atcgcttccg gcgcggtgcc ggtcacgacc
    12541 acgatcggcc gctatgtcga actggtcggg gacggccagc gcggcctgct cttcccggcc
    12601 ggggacccgg tcacgctggc cggccagatc cagcggctgg gcgaggattc ccggctgcgt
    12661 gaaggcttcc gggcgcagtg cggcaagttc gaggttgaaa gctggtccgg ggtggccggg
    12721 cgattcgagg cgatctacga gcggctggtc tcccgccgcc atgatccggc cggcaacgaa
    12781 gaactgagga agaagctgcg cgaccggccg ctgatcgagg tcgacctcca catgcacacc
    12841 gaccattcga gtgactgcgc gaccccggtc aaggtgctga tcgaaaccgc ccgcgaccgc
    12901 ggcttcggag caatcgcgat caccgaccac aacgaggttt ccggggccat ggaagccgcg
    12961 gaggtcgcgg cggagatgga tgacttcaag atcatcgtcg cggaggaagt gatgaccggt
    13021 gacgccggcg aggtgatcgg cctcttcctc aaggagaaga tcccgaaggg aacctcgatg
    13081 tcggacacga tcagggagat caagcgccag ggcggcctcg tctatgtgcc gcatcccttc
    13141 gaccgcctcc actcggtccc cgattacgag aacctgctcg acatcgtcga ggacatcgac
    13201 ctgatggagg tcttcaaccc ccgcgtcgcg atctccgtct tcaacgagga agccgaacgg
    13261 tttgcccgca agtacaacat catcccggcg gccggctccg acagccatgt cgcccagggt
    13321 ctgggggcgg ttcgcaaccg cctccatgat ttcgacgggc ccgaggagtt cctcgaagca
    13381 atgcgcgagg cggagatcac ccgccgccac aagaacctcg tttacgttca ggcgctgaag
    13441 ttcattcaga ccaccgggcg gcccaaggcg gccaagcgtc aggtcgatga cccgcaggcc
    13501 gtcagaggcg gccggcgcaa ggcccgtggc tcgaagcagg cgcgtgggcc aaaacgtgca
    13561 gcaggcaaga gctgatgcac ggacccggtg acagccggga tccggcagga gccccgccgg
    13621 gggcgtctaa taaggcatca gcgatgcctg cgaccgaaaa cgagatcaaa gagaagtatc
    13681 tggaacgcgc gatccgggag ctgaatcagc tgaaccggga tctgcagggc tgtgacgcct
    13741 gtctgaccac gaaggtgatg ccggtgctcg gctccggtca cccgcaggct gacgtgatgc
    13801 tggtcaagtt cgccccgacc accgccgagg tggaggaggg cgtggcgttc tacggccggg
    13861 ccggcaacgc cctgatgaag tccttcaaac gactcaacat cgacccgacc gttatctacg
    13921 gcacggtctg cgccaagtgc ccggtcgagg atccgacccg ggccgacccg gaatgcgtgg
    13981 cccgcctggc cgacgagatg ggcatcgtcc agccccgcat cgtcgtcgtg atgggtgagc
    14041 ccgccctgga gacgatcaac agccttgccc tgccgctggc ccgtccgctc gagccccgcg
    14101 aaggcgagat ccaggccttc agccccgcct gcgacgccct ctttaccccg gacatcgacg
    14161 gtgccctcga cgaagaaacc gccaagcgcc gcttctggcg agccttccgc accctcggcc
    14221 gctggcacga cgacctcccc ccgtactaac ccgcccggca ccagcacctg ccggaaaccg
    14281 ccccgccttc accgccccgc cggcccgctc cggccccgcg agattccact ttgcgccaca
    14341 taccggcaca aaccgggcac tcggtgactt ctttagggga tgtcggacta aaattccggc
    14401 atgggaaccg tcgaacaaac ggtgatcggt ccccgcgagg gccatgcgaa cggcaaggcg
    14461 ctcgcggcga tgatcctcgg cgtcttcgcg atcgccaacg tggtcgtcct ggccctcttc
    14521 atggccctga gcgccgagac gcttgacgac agcggcgcct ggtggatgct gctcccgttc
    14581 ttcggcctca ccgccggcct gatcgcggct tttctcggat cgctcggctg gatcgacgtc
    14641 cgccgtggca tcaccgaccg ccgactcttc gaagcccagc tcggcgcgat cctcggtggc
    14701 gtcgcggccg gactgatcct gctcgcgatc accgttctcc tggtgatggc cctcttcctc
    14761 gccttcagct tcgccggcga gggcggggtt gactgatcac cccggccggg gcaggttcag
    14821 tcgctccacc agccgggctt gacgtcgccg tcgcctgaca tgtgctggcc ggcggcgtag
    14881 tagaccatcc cgtcgggtcc ggcttcgaag cagcggatga tttccggagc gacccggatc
    14941 aggtcgcctt cccggagctc gacgatgtca tcgtccagct tgacccggcc cgagccggaa
    15001 accacgaagt agacctcctc ggcccggtcg tggacgtggc cgaagggctg gcgctggttc
    15061 ggatgcatgc ggaaccaggc cacgcccacc tggcttgctt ccaggtcacc gatcgcgaag
    15121 cgggcctcct ggatctcgcc gagaccgtgg tcttttgccc cgtcgtttac ttccgcgacg
    15181 ttgaccagct tgtatcccgg cttcggggtg gcggcacggg ggtcgtccag cctggcctcg
    15241 attccgccga gcggcagcag ctcgatcact tccatgtcct cgatcagggg gcgcgcccgg
    15301 tcgatcaggg tcttgttttc ctcatcctcc agcgaggcct tcaccgcatc ctggtcaacc
    15361 caggcctcgg tcacccagat cgtgtccggg tcgaagacgg actggtgcac ctcgtactgg
    15421 aggcagcctt cggaagcgcg gttgacctcg gctgcctcaa gcagcaggtc ggcgagttca
    15481 cctcccttgc cggctttcgc ggttgccttg gcgtagcggg agatcttctc tcggtcgggc
    15541 atgtccggac tctacccgcg aatcgcggcg accaagccgc gaccgctcag agcccgtcgt
    15601 ccttggtcgg cgagaggtaa cggtcgatgt aggcgagacc gatcgccgaa aggacgaagg
    15661 tgaaatggat gatggtctgc cacatcacct cggtggaggt caggcccgtc tcttcggttc
    15721 cgtactcgat gaaggtcttg agcaggtgaa tcgaggagat gccgacgatt gccatcgcca
    15781 gcttgacctt gagcacgttg gcattcacat gcgaaagcca ctccggctgg tcggggtggt
    15841 tctcgacctt gatccgggaa acgaaagtct cgtagccgcc gatgatcacc atgatcagca
    15901 ggttggcgat catcaccacg tcaaccagac cgagaaccgt cagcatgacg gcgttttcgt
    15961 cgaagctgct gaagtcggtg atcaggtgga ccagttcctt ccagaacttg accacgtaga
    16021 ccgcctgggc gacgatcagg ccgaggtaga gcggggcctg gatccagcgg ctggcgaaaa
    16081 tcgaccatcc gatcatgccc gccggagtgc ggggacgggt cacgtccatg ccgggacgtt
    16141 catcggaggg aggggaggga ctgggcgcag ggtcgagcgg aggtgcggac atcgccgcaa
    16201 atccaaccag agcgaccggc ggaaaaccgc tctttatcgg gatttgcggg ggtttatcag
    16261 gcgggtgccg cggccctggc gagctcgacc atcggctcga ccttcgccga ggcgagaccg
    16321 cgcaccacga tcacgtcgag cgcctcgtag gcggccagct tctcctgggc ttcggccgaa
    16381 tcggcggccg ccaccccgac cgttccctcc ggctcgccga ggcgggcgaa gtggttccgg
    16441 tagccgtcgt gaaggtcgcg gtaaaaggac tcttcctttg ccaggcgctc cgccgcatcg
    16501 gggccgaccg cggtgcggac ataaccgaag acgggcgggg cctcgtgccc ggcatcggcg
    16561 gcgccggcct cgaccttggt ccgggcgccg gcggcgaact ccggggtcat ccagttgaag
    16621 aaggcgccgt cgtactcgga gccggccagg gcacacatct tcgggcccat cgcggccatg
    16681 atcacccgga tcccgggcag cttctcccgg agctctccga tctgctcggt catgaaagtc
    16741 agcggcttct tcgtgaatcc ggctccgacc ccgatccaga ggcgggaggg gtccagcccc
    16801 agtcgttcga cgtcggccgc gatctcggcc gggctctgcc ggtcgagggc gatcacggcg
    16861 accccgagct cgaccccgtc ggcacccttg gcgaactcgg ccagcgtttc gagccccttg
    16921 gccaggggat ggtcgttggc ccagaccgag gcgtagccgg cctcctcgca ggcggcggcc
    16981 aggggggcgc agacctcggc cggaagtccg gcggcaactc cgaaagcgcg cttctgttcg
    17041 ggacgggtca accttcagcc tccttcgcgg cctggcgccg ctcgtctcgt tcacgacgtt
    17101 tgcggtcggt gggggattcg acgtacttca cctcgccgac cagatcgaac tgggcgacgc
    17161 actcgatgtg cggggtctgg ggaaacatgt caaccggcct cacccgggtc agcctataac
    17221 cggcctcgac cagctgggcg gcgttcgggg ccagcgtggt cgggttgcag gagatgtaaa
    17281 cgatccggcg ggcttcgcat tcgatcaggc ggcgcacgat cttggccgag agcccggccc
    17341 ggggcggatc gatcgtcacc acatcgggcc ggccggcttc ctcgatcagg gggcgcatcc
    17401 cggtgcgggc gttggcggcg acgaaacggg cgttggttat gccgtttgcc ttcgcgttcc
    17461 gcttcgcgtt ctcgatcgcc tcgggaacta tttccaggcc ccagacctga ccggcctgcc
    17521 gggccatgct gagcccgatc gtgccgactc cgcagaagag gtcgaagagt ttctcgttgc
    17581 cggtcagccc ggcgtactcg gcggcgaggc cgtagagctt ctcggccatt tcggtattgg
    17641 tctggaagaa ggcgtcgtgg gagagctcga tgtcgaggtc gaagatccgc tcccgcagga
    17701 cttcctcgcc gagtaccccg gtgggtccgg aagtcgaacc ggagggaccg tcgatgatcg
    17761 tgtgaaggtc gactggcggc ttggggaagt tggcgcgcga ggtgaccagc cgggtctgga
    17821 cctgcccggt ccggcggcct tcgcggacga ccaggttgcg caggatgccg cggtggtcat
    17881 gctggtcgta cgcgttgagg ccttcggccc gcgcccaggc gaggacttca ttgcgggccc
    17941 ggttggtcgc ctcggaggag aggtgacagt cctcgatgtc cagcacctcg tcccagcgac
    18001 cacgggggtg gaagcccagc accagctcgc cgtcctgctc gccaaatgag tattcgacct
    18061 tgttgcggta tcgccactgc tcgtcggcct cgacgatcgg gtcgatgttg acgtctccga
    18121 gcttgccgat ccgggccagg gcgtcggcga cgtgctcctc cttgaacgcg agctgcttct
    18181 cgtaactcag cgcctgccac gacgcgccgg ggcaggcttc accctggtgg aggtcggtgt
    18241 cggggagccg gtcgggactc ggggtgatga tttccagggc gttggcttcg gcgtagcccc
    18301 gtttcgactt ggtgatcaaa gcccgcaccc ggtcaccggg cagcccgccg ttcacgaaca
    18361 cgacataacc atccggtttg gtgatgccgc ggccgccgta ggcgagggtt tcgatctcgc
    18421 cttcgaaggt ttcgccgcgg cgcagtcggg tgggagggct ggagggctgt gttgcagaca
    18481 ttggacccac cactatctca ggctgaaagc ggccttagta ttcccctctt gactgaaccg
    18541 cgtgccaggc aatacatcga cggccacgag atctccggcg gccacctcgt caacctgatc
    18601 gatcgggccg aggagctgag ggccggccgc cccaatgagg ggggctccgc cctggccgga
    18661 aagtcggtcg cgatgctgtt cgagaagccc tcgacccgga ccaggatctc gtttgaggtg
    18721 gggacggtgg aactcggcgg ccacccggtc gtcctgcgcg gcagtgaaat gcagctttcc
    18781 cgcggcgagt cggtggaaga caccgcccgg gtcctttccc gtttcgtctc cgcaatcgtg
    18841 atccgctcgg gcagccacga gaacgtggtc gcgctggccg agaacgcgag cgttcccgtc
    18901 atcaacgccc tcacccccga gtttcatccc tgccaggcgc tggccgacct gcagaccctc
    18961 aagcagcgct tcggcaccct gaatggcctc aaggtcgcct acgtggggga cgggaacaac
    19021 gtcgcccgct cactcgcgat cgccggctcg ctgaccggag tggaggtggt tgtcgccgct
    19081 cccgccggct atcagctgga gaccgaggtt cccgcgaagc tgaccgatga tccgatcgaa
    19141 gcggttcacc gcgccgatgc gatctacggc gacgtctggg tcagcatggg cgacgaagcg
    19201 agcgccgggc aacgccgggc cgacctcgct ccataccgga tcgacggcca tctgctttcg
    19261 cacgccaggc aggagacgat tgtgatgcac tgcctgccgg cccatcccgg cgaggagatc
    19321 accgccgacg tgctctacgg ggatcgcagc gcggtctggg accaggcgga gaaccgcctc
    19381 cacgcccaga aggccctgct cgagtacctg ctcgccccct gacggctgct cgcccctgac
    19441 ccctgctcgc ccctgacacc cgctcgcccc tgacacctgc tcgccccctg gcctcgggat
    19501 ctgcccgaat cgagagggct aaatggagcg ctatatggcc acttttgcct tctcgcgcgg
    19561 ccggggaacc ggtcggcaag ccgccactcg cgttcaactg agagaatgtc cgccgcgcgg
    19621 ccccgtggtg taacggttag cacgccaggt tttcaacctg gaagcgcggg ttcgactccc
    19681 gccggggcta ctgctcgccc tttgccaggg ccggcgaagc ggtccggccg gaatcgcagg
    19741 cctgacccgc accggtaggg tccggggcgt gattccggag atctcgctcg gcccgataac
    19801 tctgcagacc ttcggtctcg ctttcgcggc cgccttcatc gtttccggat ggatcgtcca
    19861 cctgcgactg gaggagatcg gcaaaccgcc ggactgggca tacgagatgg tcttcgccgc
    19921 gctgatcggc ggtttgatcg gggcccgggc ctacttcatc ttcgacaact acgccgacgt
    19981 caaggacgac ctgctcggca acatcttcag tggttccggc ctgacctggt acggcggcgc
    20041 gatcggtggc gcgatcgggg tgatcctctg ggcctggtgg cggaagttcc tcaacctgtg
    20101 gcttctggac accgccgcgc cggctctcgc agcgggttat gcggtcggcc ggatcggctg
    20161 ccagctctcc ggtgacgggg attacgggaa gccctgggac ggaccgtggg cgatgagcta
    20221 cccggacggc accgtcccga cccccgacgg tgtcaccgtc cacccgaccc cgatctacga
    20281 gaccctgacc atggggctgg gcgcgtacat cctctggcgc ctcagggacc ggttccggct
    20341 ggggatcctt ttcgcgatct acctgatcta cgccggaacc gaacgtctcc tggtcgagtt
    20401 cctgcggcgg aacaccgacg tctttctcgg gctcacccag gcccagctcg agagcgtggc
    20461 gatgatcctc ggcggcatca tctggatcct ggtcgtcagg cgccgctacg gcagcctcgc
    20521 ccgcccggct gaccagatga gcgctgccga cgtccccgcc tgaccgtacc gcgaacgacc
    20581 gggcataatc ccctttccgg ttcgcgcacc ttccttcagg tccgcggacc ccggggcggt
    20641 tagctcagtt ggtagagcgc ctgctttaca cgcaggatgc cgggggttca agtccctcac
    20701 cgcccatc
//
LOCUS       DAOQKN010000019        20503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_1142_length_20503_cov_5.949090, whole genome shotgun sequence.
ACCESSION   DAOQKN010000019 DAOQKN010000000
VERSION     DAOQKN010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 20503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20503
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1142_length_20503_cov_5.949090"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="PLJ64_02480"
     CDS             complement(<1..570)
                     /locus_tag="PLJ64_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92390.1"
                     /translation="MPLLKDYFTRYGRGALIALAVIACAGFLVRAWVVVNPLADPGDD
                     ALAYRALAEALYVDGTFGYGKDSSSGPEFKSPSDWSPGAPLIYAGSYYLTGGVRDGVA
                     RGVEALFGTAAILLAFLLTARLLGPPRRRNWRHALPPLVAAGLVAFYPPFIHSTGALM
                     SEPPAFFMLPASVLAFLWADRRMGEATGSG"
     gene            complement(580..1947)
                     /locus_tag="PLJ64_02485"
     CDS             complement(580..1947)
                     /locus_tag="PLJ64_02485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNC92391.1"
                     /translation="MRVLALILVVMAGLALRMDAAWQGAAENMPDSAAYERIARGLHE
                     DGVFAQKGPGTPAHPQPATNYSPGLPLAVAGVFDLAGGDNVRLARLLLALVGTLSIPL
                     AWLLARRLAPPEDPGVAGIVAAAAVAFYPCLIADAGMLLTEALAGTLVTGALLSMLRA
                     RDHLGEPGRPLWLRTTDWLLPGALLGMTAMVRPEYLPISLALILVLALISRGHGLRPV
                     LFGSALMLLAVGLVITPWVTRNLDHSGRLVPLSTGGGQTLFTGSYLASAGNPQAVMPD
                     LLARNPDLARRIQVQNDASGEGPASITPERVLALLAAERMPGVPTDVALARIGRENYL
                     RALQQDPARLLGYFAAKSARIWWRGRKDLTGTTTGLVSHMTLVAGALLGLLLLGFRRQ
                     PEFWLILALAVGVTLIGAVFVASPRRTLAFWPVIACLSGIGFSLALRLARDAIAARWR
                     PVPIA"
     gene            2048..3241
                     /locus_tag="PLJ64_02490"
     CDS             2048..3241
                     /locus_tag="PLJ64_02490"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNC92392.1"
                     /translation="MRILILGGDGYLGWPTAMRFSDRGHDVLIVDNFARRRWHVDQST
                     DSLTPIASFEDRIEAWERVSGRRIETRVGCIEDGEFLDKVISDFLPEAVIHYGEQPSA
                     PYSMRSRQTAVETQQTNVIGTLNLLFSMREHVPDAHLIKLGTMGEYGTPNIDIEEGFI
                     EIEHNGRKDTLPFPKLPASLYHCSKVHDSTNIHFATRVWGLRATDLNQGVVYGIETDE
                     TALDPALATRFDYDETFGTVLNRFCVQAVIGHPLTVYGAGGQTRGYLNIRDTLRCVEL
                     ATLNPAERGEFRVFNQFTEHFTVAELAQLVQRCAAEVGHEVSIETFPNPRVELESHYY
                     NPKNQKLLDLGLEPHYLGEELVRSVLSTIEQYKDRVIPRAITPKTKWNPGELEGGALS
                     AAQKT"
     gene            3278..4957
                     /gene="ilvB"
                     /locus_tag="PLJ64_02495"
     CDS             3278..4957
                     /gene="ilvB"
                     /locus_tag="PLJ64_02495"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HNC92393.1"
                     /translation="MRAADALFRSLEAEGVDHIFGIPGGANLPTYDALVDADLRHVQA
                     RHEQAAGHAAEGYAHASGKVGVAFGTSGPGATNLVTAIADAMMDSVPTVFVTGQVRTD
                     LIGTDGFQEADIVGITLPIVKHSLLVTEPEQIPEFVHEAFHIASSGRPGPVLIDIPQD
                     LARAEIDYTPRTEPVDLAGYKPTVEGNLKQIRLAAKAMANARRPVIYSGGGVKNGNAS
                     MELRELCATDNFPVTSTLMGLGCFPADSDQWLGMLGMHGTRTANYAMDEADLIIAIGS
                     RFDDRITGKLSEFAPSAKFIHIDIDPAEISKNVPAHIPIVGDVKQILPKLTREYQALQ
                     TDSARLEGWWSRLKAWQGEYPLTYEPGADGEIKPQFMIEMMHKVTGGDAIVTSDVGQH
                     QMWTAQHYGFTQPRKWINSGGLGTMGFGLPAAIGAKVACPNEQVVCIAGDGSLIMNIQ
                     ELATAVNEDVPVKVFLMNNGFMGMVRQWQELFWSERYSGVENGPSPDWVKLSEAFGAG
                     GLRCEDSADLEEMMIATLEHDGPALLDVRVSPRENCYPMIPAGNAARDMVG"
     gene            4961..5503
                     /gene="ilvN"
                     /locus_tag="PLJ64_02500"
     CDS             4961..5503
                     /gene="ilvN"
                     /locus_tag="PLJ64_02500"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HNC92394.1"
                     /translation="MASTDMVRLEDLHAGRSPLSGRKHVLSILVENRPGVLARVAGLF
                     SRRGFNIDTLAVGPTEDPEVSRITLTLDGAIHPIDQVTKQLHKLVNVLKIRDMEPEET
                     VAREMALFKVSAAVENRAEIVQFAEIFRAKIVDVSRRALIVEVTGSHDKIEAFESMLR
                     PHGLLEMVRTGEVALARGGD"
     gene            5503..6906
                     /locus_tag="PLJ64_02505"
     CDS             5503..6906
                     /locus_tag="PLJ64_02505"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HNC92395.1"
                     /translation="MPTPADRATLETDLREALAEASARRSKTVVSVTLPYGCADPIAT
                     VFASRRAGEPFFCWEQPDRNLRLAALGRVHEITSRGPDRFVEVAADCAHLSGDAVLRG
                     EGGFTTGALWTGGFAFHDEGTEAGVDPAWSSFEPATMILPELAIQSSDAGNFITVNLM
                     AGPGADPAALADSALVRIAGLVHPELPMIDPHPTVAASIESVRSPEDYEQSVAAAVER
                     IRAGEIEKVVLAREVVVRSGSAHDPAAIFGALRIGFPSCFNFCVGTGEATFIGASPEL
                     LVRCAGRGVSTVGLAGSTRRSSDPAVDDHLAQQLLGSSKDRGEQAVVVRRIVKSLSKL
                     SVWVEAADEPEIIKVANIQHLGTPVYAQLAQPRTAIELAGLLHPTPAVGGEPWKKARR
                     VMAEVERMDRGWYAGPVGWMDGSGDGEFCVALRSALIRDREAHLFAGVGVVADSDPAA
                     ELAETEIKLGALLPLLS"
     gene            complement(6972..8666)
                     /gene="menD"
                     /locus_tag="PLJ64_02510"
     CDS             complement(6972..8666)
                     /gene="menD"
                     /locus_tag="PLJ64_02510"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HNC92396.1"
                     /translation="MALCTAFAEELDRSGVRLAVISPGSRSTPLALAFFRQDGIETEI
                     GLDERSASFYALGAAQASGTPVVLVCTSGTAAANFHPAVAEADLSSVPLIVLTADRPP
                     ELRDIGAGQTIDQIKLYGGSVRWFAEVGNHAADDAGLLHFRSLACRAHATANGDPRPG
                     PVHLNFPLRDPLAPVPEDEAVTATRRLALEGRPGGPLTTVEKASCGADRSTVNRLVSM
                     VQKAERILILAGRQTDPSIREPLARLANACRAPVLAEPTSQFRLGPHDRGAVVTAYER
                     IARRREKGLAPDLIIRLGEFPTSKQLRLWTAETDPAEIVVDPGYGWYEPVRTADLIVR
                     CDPSTLLHQIENTAEQRVGGPAGSGFWNKWMEAERKERAEIEAAWDPAGGSEGPLEAG
                     AVHARLAELQTDGNLVYTASSMAIRDQEESLPALDRDVFFLANRGANGIDGLIASGIG
                     AAKATGRPVTVITGDLGFFHDVGSLALARGLEVPVRIVVLNNDGGGIFERLPQKQAMT
                     GGEFEQLMRTPSGLSVEDAARLYGLPFHLAPRLDALEAAFTADAESVSVIEVPVTG"
     gene            8896..9063
                     /locus_tag="PLJ64_02515"
     CDS             8896..9063
                     /locus_tag="PLJ64_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92397.1"
                     /translation="MSHRSAFRLSLFATLFAIFGLTAQIASAATLRVDNDKQQCPAAA
                     YTSVQAAVDAG"
     gene            9063..11987
                     /locus_tag="PLJ64_02520"
     CDS             9063..11987
                     /locus_tag="PLJ64_02520"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNC92398.1"
                     /translation="MGDTVIICDGTYSEGSGQVGSNAVTITKSLTIKGAGADKVTIRP
                     TRVDPARQIASASPDLRDGVGSIIAVNGAPATPITVDISGVTVSGGGTEAFLPSSSIW
                     NGEFLNGVYAEAGVSFLDAGGSLNASRVTNIVTSNDPANAEPGGYRSNNVGWGVTQVT
                     AAGGSGTATLPISLIGTRIDRYNKGGLLVSGSTGDTPPLTRAGTIGQATVTASSIIGR
                     NLNYPANDGTAPGTLVSTGAVFGQDGIQVTSGSTLTATSNLISQNLMAGTGTATAAAI
                     RGAAGIRLIDIGTNTVATKSNILYNGYGIINLDADGVTDNTTTTIDAPLNYWGMSDAS
                     GTVNTGPAVSPAAVPAYPGNPVNGAADVTYGSNSVHFLPFRSGPKTEPGTIANPELGG
                     QWPQADAPIPVADSAPTSALSSNTDTVAPGGTLTLTAHANDDFGVKSVTFYKGATPLG
                     TVVPPNDSITWDAPATCGGSNQSFSVTVVDSNDQSSSSSLSVAIDDCDPAVSLSAAPT
                     APLGTIRLSADASDDLGLASLDFYVDGDLIHTVSNPDNGISTYDYVPETLCGADEFGF
                     EVVATDSTGHQTADSVNYSKDDCDPVVTLAANHASVAPGGAVDLIAEAEDDVEVAQLT
                     FFKAASQLASYPNTDHTAAWVKVHSTTASNLCGTTDHFRVVAEDNAGQTSEDTVDVST
                     TACPDNPPTIDLGASATTVDPGDDVTLTATASDDHGVSSITFFEGATQIGQITPPANS
                     IVWTAPETCDTSYTLKAVARDTANQTTEDTVMVTVNACPPPAEPTVSIDSPPAQIAQN
                     GTTVSATATSGADVSKVTFFLGIRQVCEDTSAPFSCKLVPNGDEVGAQPIKAVVTDSL
                     GRTAEDNESTTVSKFKPKGLSLSAKRIGSKKRRIKASGKLMLPARMTAADGCSASRVA
                     ITPKLGKKSLVNKQVKLASNCSYSLAFGAPKTKKKQRVVIKARFAGNDSLSATSQVRK
                     VR"
     gene            11984..15022
                     /locus_tag="PLJ64_02525"
     CDS             11984..15022
                     /locus_tag="PLJ64_02525"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HNC92399.1"
                     /translation="MIRLKTIEHDSNGLITGSKNMISMRKKITGVLAALVAAIALTGV
                     FAGQAQAITYDASTCTPEISYDPAIPTPQEFFGGPLAPNTGGDNTSNNAAKKNTTVLY
                     PYLTALANAAPEMIQKRSAGTTALGKEIFFLIISSPDNMNDLESDAAFWRAVREGEIA
                     PDEAKKAVFNRPAFAWIASNVHGDEASSAEATVRMAYELAARRDCANYQRLTNLTSFL
                     LPVQNPDGRDAYARTNAWAFDLNRDWHTQEQAENYLKMGSALQYPSVVYVDAHQQGGN
                     SFFFPPNEDPVHHEISNAAMNAINFIYGPALQKRFNDQNIAYTNYNSYDMFTPEYGDT
                     APSLLLGAAGMTYEKGRGQAYSKQVYDHYLSLDETLNTTSRLKQKILGAWVDQWYEAI
                     AQGAAGELEPNQIVSPSHSITWNVPSEKVYGYFFKPNNHDGDVAKMISVLQRAGVKVY
                     KLNSRIAVPGIHINGDVTVNSTTELGPTRQTKNTQTMDLPAGTLYIPMAQSMKHWIQA
                     LLGEDPFVPYAYFYDVTGWSFSQLKGLSGDGSLTKPMPADASLSLIAAPDLGGAPASA
                     TKYYAFNTDSSRALVMLYKLAGANVKVYRTVAGFTLGGKKFPTGTAILDGSTVTANGI
                     NLETYSDTYQTPITGMSVLPSVNRYLIKKPKLAMLTGTTAVTLPPAGRCDIGTTPCQA
                     MFVLREKLGLPVTPVTTTQLAAGELVSGQYTAVVNPATTVAAGAGATALQTFVNNGGS
                     YFSWGTGGATSARNAGMTNVNTTTNGTTDGVPVKVNFNTNSPLSWGFDNGGFLYRVGS
                     GAVTYNPTTLTGNGGSVPNAVEAASYPNPTVRFSYATVAGMTNLFGKTAAVDQAFGAG
                     RVTLLSIDPTFRAWLEGAERLVLNGILYPTGSVITPAGHAAPVTVPAGAIEPGEGEKV
                     TRKELPKVKNRPAKKTHDPANDVRITVGVRQARNLKGAFRAVAPARIKKIARFSKAKN
                     GNVTLIVPNAHSKDGHFAVWVQKLGARLQANGIQPKAIRL"
     gene            complement(15036..16079)
                     /locus_tag="PLJ64_02530"
     CDS             complement(15036..16079)
                     /locus_tag="PLJ64_02530"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HNC92400.1"
                     /translation="MNDTGVGPLSVEVIPFAMRFRRPYVTAAGVLARRESVLLRLRDE
                     NGLTGLGEGVPMSLRGGDRLELVVAELEAWADDPGLLPASAPARCAVSMAVADLVAKR
                     EQVPLWQYLDPAATPRTLKCNATITSGEVSDVVTQCETWAADGFEVFKLKAGPGEALE
                     LAKAVRSALGHEAKLRIDANGTWGESAGDLLKALEPFGLELVEEPVTGLAELAALSRG
                     TGIPLVADESVNDPEEAADARFEQACEAATVKLSKIGGLDASLGGHLPTYLSSALDGP
                     VGIAAAAHVAQTLDPNLPWPGMAHGLATERLFEQTLSATGPLTALNELDPPANGFGLG
                     VELDEGILAACRL"
     gene            complement(16081..16962)
                     /locus_tag="PLJ64_02535"
     CDS             complement(16081..16962)
                     /locus_tag="PLJ64_02535"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HNC92401.1"
                     /translation="MRIWLNAARPRTLPAAIAPVLVGTAAAIYVAGDLPRLGVFLAAL
                     AASVMIQIGTNLANDYSDARRGADSADRLGPVRVTSAGLITPQRVLTATWIAFAIAIL
                     LGFYIAWRSDWIIIAVGVISIAAGFLYTGGPRPYGYAGLGEVFVFLFFGLVAVNGSYF
                     IQLEELALLPFGLSVSIGFLSTAILVVNNVRDIDSDRRANKNTLAVKIGREKTRRLYV
                     FLVLAAFFVLGLTIVLNDGPWLALIGVIAIPMARDPIAAVTGRTDGPSLNKALAGTGA
                     LLGATSILTALGLVLGA"
     gene            complement(16959..17786)
                     /gene="menB"
                     /locus_tag="PLJ64_02540"
     CDS             complement(16959..17786)
                     /gene="menB"
                     /locus_tag="PLJ64_02540"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HNC92402.1"
                     /translation="MEWRSAGEYSDILYEKAEGIAKITINRPEVRNAFRPQCLAELRD
                     AFEKARDDTEVGAIIFTGAGKEAFCSGGDQRIRGDDGYIGDDEVAQQGVGRLDVGDLH
                     VQIRRTPKPVIAMVAGYAIGGGHILHLVCDLTIAGDNARFGQTGPRVGSFDGGFGASL
                     LARNIGVKRAKEVWFLCRHYTAEEALDMGLVNAVVPVDDLEKETVAWCREMLHLSPLS
                     LRLLKSSFNAEEDGLTGLQQLSHDATLLFYMSEEGQEGRNAFQEGRKPDFNKFPRRP"
     gene            17920..19251
                     /gene="menE"
                     /locus_tag="PLJ64_02545"
     CDS             17920..19251
                     /gene="menE"
                     /locus_tag="PLJ64_02545"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR01923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HNC92403.1"
                     /translation="MKLDNWLAQRSQSCPDRIALIADGESMTYAQLEEEAVHAARRLA
                     AWGVRRGATVAMNMRPGRDKIILVHALMKTGAVLLPISPKLSPEERAKAIESCDVSVD
                     LDDPGKLTQTEADMPLLGEHDMSDPHCRILTSGSTGRPHTVSLSYGNHLFSAMGSAFN
                     IGVDPSDRWLCALPTSHISGLSIVMRSVIYGTAMVLQDHFEPEAITDAIERDGATVIS
                     LVSTMLLRLLDEGVDLTAPRAILVGGGPVPASALSEALDRGARVIQTYGLTETCSQVT
                     TLEVAEARRKVGSVGRPLLTSHLRIQGGEILVQGPTVSAGALDADGWLHTGDVGWIDE
                     DGFLYVQGRTDEMIITGGENVIPAEVEDVLVQHPEILEAAVIGRPDPEWQQAVTAVVV
                     ARDGVVVDLDGVRSHCEGKLAPYKIPKAIELVQELPKTPSGKLHRAALLKS"
     gene            19310..20314
                     /gene="ilvC"
                     /locus_tag="PLJ64_02550"
     CDS             19310..20314
                     /gene="ilvC"
                     /locus_tag="PLJ64_02550"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNC92404.1"
                     /translation="MSTQDIFYDKDGDLSKLEGKTVAILGFGSQGHAHALNLKDSGVN
                     VVVGLREGSASREQAAAAGLEVLSVADASSKGDIVMILLPDEKQAEIWENEIKDGIAE
                     GNLLMFAHGFAIRFDQIVPPAGVDVGMVAPKGPGHMVRRQFEEGSGVPCLMAVEQDAT
                     GNAHDLVLAYAIGVGGGRAGIIETTFKDECETDLFGEQAVLCGGATELVRAGFDTLVE
                     AGYDPRLAYFECLHELKLIVDLMYEKGIQGMRYSISNTAEYGDLTRGKRVIGEDSRAA
                     MKEILGEIQSGEFAKEFIGENQAGNENFDRMRQEGKDHKVEIVGKELRDMMPWINQEF
                     "
BASE COUNT         3736 a         6906 c         6687 g         3174 t
ORIGIN      
        1 gccgctaccg gtggcttcgc ccatcctgcg gtcggcccag agaaaggcga ggaccgatgc
       61 cggaagcatg aagaaggcag gcggctcgct catcaaggcc ccggtcgagt ggatgaacgg
      121 gggatagaaa gcgaccagtc cggcggcgac caaaggggga agggcatgcc gccagttgcg
      181 ccggcgcgga ggaccgagca ggcgggcggt caggaggaag gccagcagga tcgcggcggt
      241 gccgaaaagg gcctcgacgc cgcgcgcaac tccgtccctg acgccgccgg tcaggtagta
      301 ggagccggcg tagatgagcg gggcgcccgg cgaccagtcg ctgggtgatt tgaactcggg
      361 tccgctggag gagtccttcc cgtagccgaa ggtcccgtca acgtaaaggg cttcggccag
      421 cgcccggtag gcgagcgcgt catctccggg gtccgcgagc gggttgacca cgacccaggc
      481 ccggacgagg aacccggcgc atgcgatcac ggcaagggcg atcagcgccc cgcgtccgta
      541 gcgggtgaag taatccttga gaagcggcat ccgggccggt caggctatgg gaaccgggcg
      601 ccatctggcg gcgatggcat ctctcgcaag gcgaagagcc agtgagaagc cgatcccgga
      661 aaggcaggcg atcaccggcc agaaggcaag ggtgcggcgg ggcgaggcga cgaagaccgc
      721 tccgatcagg gtgacgccaa cggccagggc aaggatcagc cagaactcgg gctggcggcg
      781 gaacccgagc aggagcagac cgagcagggc gccggcgacc agggtcatgt gggatacgag
      841 gccggtcgtg gtcccggtca ggtccttgcg gccccgccac cagatccggg ccgatttggc
      901 agcgaaatac ccgagcaggc gggcggggtc ctgctgcagg gcgcggaggt agttttcccg
      961 gccgatgcgg gcgagggcca cgtcggtggg aacgccgggc atgcgctcgg cggccagcag
     1021 cgccaggacc cgttccggtg tgatcgaggc gggaccttcc ccgctggcgt cgttctggac
     1081 ctggatcctt ctggcgaggt cgggattgcg ggcgagcaga tcaggcatca ccgcctgcgg
     1141 attgccggcc gaagccaggt agctgccggt gaaaagcgtc tggccgccgc cggtggagag
     1201 cgggacgagc cggccggaat ggtcgaggtt ccgggtgacc cagggagtga tcaccaaccc
     1261 gaccgccagc agcatcaggg ccgacccgaa caggaccggt cgaagtccgt ggccacggga
     1321 gatgagggcg aggacaagga tcagggccag cgaaatcggc aggtactcgg gcctgaccat
     1381 cgcggtcatt ccgagcaggg ctcccggcag caaccagtcg gtggtcctga gccagagcgg
     1441 acggcccggc tcgcccagat gatcgcgggc ccggagcatg ctcagcaggg cgcccgtgac
     1501 cagggtgccg gccagcgctt cggtcagcag catgccggcg tcggcgatca ggcaggggta
     1561 gaaggcgacc gccgcggccg cgacgattcc ggcgaccccg ggatcctccg gaggggcgag
     1621 ccttcgggcc agaagccagg ccaggggaat cgaaagggtc ccgaccaggg cgagcagcag
     1681 gcgggccagc ctcacgttgt cgccgccggc caggtcgaag actccggcca cggcgagggg
     1741 cagaccgggg ctgtaattgg tcgccggctg ggggtgggcc ggcgtgccgg ggcccttctg
     1801 ggcgaataca ccatcctcat gcaggccgcg ggcgattcgc tcgtaagcgg ccgaatcggg
     1861 catgttctcg gctgcccctt gccaggctgc gtccattctc agggccagtc cggccatgac
     1921 cacgagaatg agggcaagca ctctcacccg ggcaatctta gagcccgtgg gcccgactgt
     1981 ctcctctatt tcaggacttc atggtcacgg ggtccggagc cctacccttc cggtaatctc
     2041 ggccgttatg cgaatcctga ttcttggtgg tgacggctat ctcggatggc ccacggcgat
     2101 gcggttctcc gatcgcggcc acgacgtgct gatagtcgac aactttgccc ggcgccgctg
     2161 gcacgtggac cagtcaaccg attcgctgac accgatcgcc agtttcgaag accggatcga
     2221 agcctgggaa agggtttcgg gccgtcgcat cgagaccagg gtcggctgca tcgaggacgg
     2281 cgagttcctc gacaaggtga tttcggactt cctgccggaa gcggtaattc attacggcga
     2341 gcagccttcg gctccgtact cgatgcgctc ccgccagacc gcggtggaaa cccagcagac
     2401 caacgtgatc ggcaccctga acctgctctt ctccatgcgc gagcacgtgc cggatgccca
     2461 cctgatcaag ctcggcacga tgggtgagta cggcacgccc aacatcgaca tcgaagaagg
     2521 cttcatcgag atcgagcaca acggccgcaa ggacacgctg cccttcccga agctgcctgc
     2581 ctcgctctac cactgctcga aggttcacga ctcgaccaac atccacttcg cgacccgggt
     2641 ctgggggctc cgtgccaccg acctcaacca gggcgtcgtc tacgggatcg aaaccgacga
     2701 aaccgcgctc gacccggcgc tggccacccg cttcgactac gacgagacct ttggcaccgt
     2761 gctgaaccgg ttctgcgtcc aggccgtgat cggtcacccg ctgaccgtgt acggggccgg
     2821 cggccagacc cgcggttacc tgaacatccg cgacaccctg cgctgcgtcg agctggccac
     2881 cctgaacccg gccgagcggg gcgagttccg ggtcttcaat cagttcaccg agcacttcac
     2941 ggtggccgag ctggcccagc tggtccagcg ctgcgcggcc gaagtcggcc acgaagtctc
     3001 gatcgagacc ttccccaacc cgcgggtcga gctggaaagc cactactaca acccgaagaa
     3061 ccagaagctg ctggacctcg gtctcgagcc gcattacctc ggcgaggaac tggtccgatc
     3121 ggtgctttcc accatcgagc agtacaagga ccgggtcatt ccccgggcga tcaccccgaa
     3181 gaccaagtgg aatccgggcg agctcgaagg cggcgcgctt tcagccgctc agaagacctg
     3241 agcccccagg cagtgattcg ttaggatcgt tggtccgatg agagccgcag acgcattgtt
     3301 cagatccctc gaagcggaag gtgttgacca catcttcggc attcccggtg gcgcaaacct
     3361 gcccacctat gacgcgctgg tcgacgccga tctgcggcac gtacaggccc gtcacgagca
     3421 ggccgccggc cacgccgccg agggttacgc gcacgcctcc ggcaaggtcg gggtcgcctt
     3481 tggcacctcc ggccccggcg cgaccaacct ggtcaccgcg atcgccgacg cgatgatgga
     3541 ttcggtcccg accgtcttcg tgaccggaca ggtccgcacc gacctgatcg gcaccgacgg
     3601 cttccaggaa gcagacatcg tcggcatcac gctgccgatc gtcaagcatt cgctgctggt
     3661 caccgagccg gaacagattc cggagttcgt tcacgaggca ttccacatcg cctcgagcgg
     3721 ccgtcccggt ccggtgctga tcgacatccc gcaggacctg gcccgggccg agattgacta
     3781 caccccgcgg accgagccgg ttgacctcgc tggctacaag ccgaccgtcg aaggcaacct
     3841 gaagcagatc cgcctcgccg ccaaggcgat ggccaacgcc cggcgaccgg tgatctattc
     3901 gggcggagga gtcaagaacg gcaacgcctc gatggagctg cgtgagctct gcgccacaga
     3961 caacttcccg gtcacctcga ccctgatggg cttggggtgc ttcccggccg acagcgacca
     4021 gtggctcggc atgctcggca tgcacggaac ccggaccgcg aactacgcga tggacgaggc
     4081 cgacctgatc atcgcgatcg ggtcgcgctt cgatgaccgg atcaccggca agctttccga
     4141 gttcgcccct tcggccaagt tcatccacat cgacatcgac ccggccgaga tctcgaagaa
     4201 cgtcccggcc cacatcccga tcgtcggtga cgtcaagcag atcctgccga agctgacccg
     4261 cgagtatcag gcgctccaga ccgacagcgc ccggctcgaa ggctggtgga gccggctcaa
     4321 ggcctggcag ggcgagtacc cgcttaccta cgaaccgggg gccgacggcg agatcaagcc
     4381 ccagttcatg atcgagatga tgcacaaggt caccgggggg gatgcgatcg tcacttccga
     4441 cgttggccag catcagatgt ggaccgccca gcactacggc ttcacccagc cgcgcaagtg
     4501 gatcaactcg ggcgggctcg gcacgatggg attcggtctt cccgccgcga tcggcgccaa
     4561 ggtcgcctgc ccgaacgagc aggtcgtctg catcgccggg gacggcagcc tgatcatgaa
     4621 catccaggag ctggccaccg cggtcaacga ggacgttccg gtcaaggtct tcctgatgaa
     4681 caacggcttc atgggaatgg tccgccagtg gcaggaactg ttctggtcgg agcgttacag
     4741 cggcgtcgag aacggcccga gccccgactg ggtcaagctc tccgaagcct ttggcgccgg
     4801 cggcttgcgc tgcgaggaca gcgccgacct cgaggagatg atgatcgcga cgctcgaaca
     4861 tgacgggccg gccctgcttg acgtccgggt cagcccgcgt gagaactgct acccgatgat
     4921 tcccgccggg aacgcggcca gggacatggt gggctgaccg atggcttcca ctgacatggt
     4981 caggctcgaa gacctccacg ccgggcgttc gccgctttcc ggccgcaagc acgtgctttc
     5041 gatcctggtc gagaaccggc ccggcgtgct ggcccgggtt gccggacttt tctcgaggcg
     5101 aggcttcaac atcgacaccc ttgccgtcgg gccgaccgag gaccccgagg tctcccggat
     5161 cacgctgacc cttgacggcg cgattcaccc gatcgaccag gtgaccaaac agcttcacaa
     5221 gctggtcaac gtgctgaaga tccgcgacat ggaaccggaa gagacggtgg cccgcgagat
     5281 ggccctgttc aaggtttcgg ccgcggtcga gaaccgggcg gagatcgtgc agttcgccga
     5341 gatcttccgg gccaagatcg tcgacgtctc acggcgcgcg ctgatcgtcg aggtgaccgg
     5401 ttcgcacgac aagatcgagg ccttcgaaag catgctgcgt ccgcatggcc tgctggagat
     5461 ggtccgcacg ggcgaagtcg ccctggcgcg cggcggcgac tgatgccgac gcccgcagac
     5521 cgggcgaccc tcgagaccga cctgcgtgag gcgctggccg aagcttcggc ccggcgcagc
     5581 aagaccgtgg tttcggtcac cttgccatac gggtgtgccg acccgatcgc gaccgtcttc
     5641 gcctcccggc gggcaggcga accgttcttc tgctgggaac aaccggaccg caacctgagg
     5701 ctggccgcac tggggcgggt tcacgaaatc acttcgcggg gtccggatcg cttcgtggag
     5761 gtggccgccg actgtgccca tctctccggc gacgccgtgc tgaggggcga gggcgggttc
     5821 actaccggcg cgctctggac cggaggtttc gccttccacg atgaaggcac ggaagccgga
     5881 gtcgatccgg cctggtcttc attcgagccg gcgaccatga tcctgccgga actcgcgatc
     5941 cagtcctccg atgccggcaa cttcatcacg gtgaacctga tggccggccc cggggcggac
     6001 ccggcggcac tggccgactc ggccctggtc cggatcgccg ggctggtcca tcccgaactg
     6061 ccgatgatcg acccccaccc gaccgtcgca gcttcgatcg agagcgtgcg cagtcccgag
     6121 gactacgagc agtcggtcgc cgccgccgtg gaaaggatca gggcggggga gatcgagaaa
     6181 gtggtgctgg cccgcgaggt ggtagtccgg tccggatccg cacatgaccc ggccgcgatc
     6241 ttcggggcac tcagaatcgg ttttccttcc tgtttcaact tctgcgtcgg gaccggcgag
     6301 gccaccttca tcggggccag cccggagctt ctggttcgct gcgccggccg cggcgtttcc
     6361 acggtcggcc tggccggatc gacccgccgt agcagcgacc cggcggttga cgaccatctc
     6421 gcccagcagc ttctgggcag cagcaaggac cggggcgaac aggcggtggt ggtgcgacgg
     6481 atcgtcaagt cgctcagcaa actgagcgtc tgggtcgagg cggcggacga gccggagatc
     6541 atcaaggtgg ccaacatcca gcacctcggg acaccggtct acgcccagct cgcccagccc
     6601 cgcaccgcaa tcgagctcgc cggtctgctc catccgaccc ctgcggtcgg cggtgagccc
     6661 tggaaaaagg cccgcagggt catggccgag gtcgaacgga tggaccgggg ctggtacgcc
     6721 ggcccggtcg ggtggatgga cggctccggc gacggcgagt tctgtgtcgc cctgcggagc
     6781 gccctgattc gcgaccgcga ggcccatctc ttcgccgggg tcggggtggt cgccgattcc
     6841 gacccggccg ccgaactggc cgagaccgag atcaagctcg gcgccctgct gcccctgctc
     6901 agctagccac cctctcgtcg agaacccgct ttgcgccaca taccggcaca aaccgggcac
     6961 tcgccgcgga ttcagccggt gaccgggact tcgatcaccg agaccgattc ggcgtcagcc
     7021 gtgaaggcgg cctcgagcgc gtcaagccgg ggagcgaggt ggaagggcag gccgtagagc
     7081 cgcgccgcat cctcgacgct caggccggaa ggcgtccgca tcaactgctc gaactcgccc
     7141 ccggtcattg cctgcttctg cggcaggcgc tcgaagattc cgccgccatc gttgttcagg
     7201 accacgatcc ggaccgggac ctcgaggccg cgggccaggg cgaggctgcc gacgtcatgg
     7261 aagaagccga gatcaccggt gatgaccgtg accggcctgc cggtggcctt cgcagccccg
     7321 attcccgagg cgatcaaacc atcgatgccg ttggcaccgc ggttggcgag gaagaagacg
     7381 tcgcgatcga gggcgggcag cgattcttcc tgatcccgaa tcgccatgct cgaggcggtg
     7441 tagacgaggt tcccgtcggt ctgaagttca gccagccggg cgtgcaccgc gccggcctcc
     7501 aggggtcctt cgcttccccc ggccggatcc catgccgctt cgatctcggc gcgttccttt
     7561 cgctcggcct ccatccactt gttccagaag ccactgccgg ccgggcctcc gacccgctgc
     7621 tccgcggtgt tctcgatctg gtggaggagg gtggaggggt cgcagcgcac gatcaggtcg
     7681 gctgtgcgaa ccggctcgta ccagccgtag ccggggtcga ccacaatctc ggccggatcg
     7741 gtctcggccg tccagaggcg cagctgtttc gaggtcggga attcgccgag cctgatgatc
     7801 aggtcgggcg cgagcccctt ctcgcgccgg cgggcgattc gttcgtaggc ggtgaccacg
     7861 gccccgcggt catggggacc gagccggaac tgggaggtcg gctcggccag cacgggggcg
     7921 cgacaggcat tggcgagccg tgcgagcggt tcccggatcg atggatcggt ctggcgtccg
     7981 gcgaggatca ggatccgttc cgctttctgg accatcgaaa cgagccggtt gacagtcgac
     8041 cggtcggcgc cgcaggaagc cttttcgacc gtggtgagcg gtccgcccgg ccgaccttcg
     8101 agggcgaggc gccgggtcgc ggtgaccgcc tcatcttccg gaaccggagc cagcgggtcg
     8161 cgcaggggga agttcagatg aaccggtccg ggccgggggt cgccgttcgc cgtggcgtgg
     8221 gcacggcagg cgagggagcg gaaatgaagc aggccggcat cgtcggcggc gtggttgccg
     8281 acttccgcga accagcgaac cgaaccgccg tacagcttga tctggtcgat cgtctggccg
     8341 gctccgatgt cccgcagttc cggtggccgg tcggcggtga ggacgatcaa cggcaccgag
     8401 gagaggtcgg cctcggccac cgcggggtgg aagttggccg ccgcggtgcc tgaagtgcag
     8461 accagtacga ccggcgtgcc ggaggcctgc gccgcgccga gggcataaaa ggaagcggac
     8521 ctctcgtcga gaccgatctc ggtttcgatg ccgtcctggc gaaagaacgc aagggcaaga
     8581 ggcgtcgacc tggacccggg agaaatcacc gccagacgca cgccgctgcg gtccagctcc
     8641 tccgcaaaag ccgtgcaaag cgccatattc cgattggtct gatccaagga atccctgagc
     8701 ctagaactct tcggagaata aggcgctccg gggtcggact tggatttcgc cgttgcatga
     8761 ggtaacttga cccctcgtac aagcgggaag gtggagcctt agcccgggga acaatcgtcc
     8821 gtagcccggg cgcaatcatc attctccacc acccaattag cgttacttcg tccatagtcc
     8881 ttagggaggg atctggtgtc acaccgttca gctttcaggc tgtcgctttt cgcaacgctc
     8941 ttcgcgattt tcgggctcac cgcccagatt gccagcgcag ccaccctgcg agtcgataac
     9001 gacaagcagc aatgccccgc cgccgcgtac acctcggtcc aggcggcagt cgatgccggg
     9061 tgatgggtga cacggtcatc atctgtgacg gcacctactc cgaaggttcc ggccaggtcg
     9121 gcagcaatgc ggtcaccatc accaagtcgc tcacgatcaa gggagccggt gccgacaagg
     9181 tcaccatccg gccgacccgg gtcgacccgg cccgccagat cgcttccgcc tctcccgacc
     9241 tgcgggacgg agtcggcagc atcatcgcgg tcaacggcgc cccggccact ccgatcacgg
     9301 tcgacatctc cggcgtgacg gtctccggcg gtggtaccga ggcctttctt cccagctcgt
     9361 ccatctggaa cggcgagttc ctgaacggtg tctacgccga agccggcgtc tccttcctcg
     9421 acgcaggtgg ttcgctgaac gccagccgcg tcaccaacat cgtcacttcc aacgacccgg
     9481 ccaacgccga gcccggtggc taccgcagca acaacgtcgg ctggggcgtc acccaggtga
     9541 ccgccgccgg tggcagcgga actgcgaccc tgccgatctc gctaatcggt acccgcatcg
     9601 accgctacaa caagggcggc ctgctggtca gcggttcaac cggcgacact ccgcccctga
     9661 cccgggccgg cacgatcggt caggccacgg tgaccgcttc gtcgatcatc ggacgcaacc
     9721 tgaactaccc ggccaatgac ggaaccgcgc cggggaccct ggtctcgacc ggtgcggtgt
     9781 tcggtcagga cggcatccag gtcacctccg gttcgacgct taccgcgacc agcaacctga
     9841 tttcgcagaa cctcatggcg ggcaccggta cggcgaccgc cgccgcgatc aggggtgccg
     9901 ccggcatccg actgatcgac atcggaacca acacagtggc cacgaagagc aacatcctct
     9961 acaacggcta cggaatcatc aatctcgacg ccgacggcgt taccgacaac acgacgacga
    10021 cgatcgatgc acccctcaac tactggggca tgtccgacgc gtccggcacg gtcaacaccg
    10081 gccctgccgt gtcgcccgcc gcggtcccgg cctacccggg taacccggtc aacggcgccg
    10141 ctgacgtgac ctacggttca aactcggtcc acttcctgcc gttccgcagc ggccccaaga
    10201 cggagcccgg aaccattgcc aatcccgagc tcggtggcca gtggccgcag gccgacgcac
    10261 cgattcctgt cgcagacagc gccccgactt cggccctctc ttcgaatacg gacacggtgg
    10321 ccccgggtgg caccctcacc ctgaccgcgc acgcaaacga tgacttcggc gtcaagagcg
    10381 tgaccttcta caagggcgcg accccgctcg gcacggttgt cccgccgaac gactcgatca
    10441 cctgggacgc tcccgcgacc tgtggcggca gcaatcagtc gttctcggtc accgtcgttg
    10501 actcgaacga ccagtcgtcc tcctcgagcc tctcggtggc gatcgacgac tgcgatcccg
    10561 ccgtctccct gagcgcggcg ccgaccgcgc cgcttggcac gatcaggctc agcgccgacg
    10621 cgagcgacga tctcggtctc gcttcgcttg atttctacgt ggacggcgac ctgatccaca
    10681 cggtcagcaa cccggacaac ggaatctcga cctacgacta cgtgccggag accctctgtg
    10741 gcgcggacga gttcgggttc gaagtagtcg cgaccgactc caccggtcac cagactgcgg
    10801 actcggtcaa ctacagcaag gacgactgtg atcccgtggt caccctcgcg gccaaccacg
    10861 ccagcgtcgc tcccggcgga gcggttgatc tgatagccga ggccgaagac gatgtcgagg
    10921 tcgcgcagct gaccttcttc aaggctgcct cgcagctggc cagctacccc aacacggatc
    10981 acacggcggc ctgggtcaag gtccactcga ccaccgcatc caacctctgc ggcacgaccg
    11041 atcacttccg ggtggtcgcc gaggacaacg ccggccagac ctcggaggac acggttgacg
    11101 tttccaccac ggcctgcccc gacaacccgc ctacgattga cctcggcgca agcgcgacca
    11161 cggtcgatcc gggtgacgac gtcaccctga ccgcgaccgc cagcgacgat cacggggtca
    11221 gcagcatcac cttcttcgaa ggtgcgaccc agatcggcca gatcactccg ccggccaact
    11281 ccatcgtctg gacagccccc gaaacgtgtg acacgagtta cacgctgaag gcggtcgcca
    11341 gggacaccgc gaaccagacc acggaggaca cggtgatggt gaccgtgaac gcctgcccgc
    11401 cgccggccga gccgacggtg agcatcgact cgccgcctgc gcagatcgcg cagaacggca
    11461 cgaccgtcag cgccaccgcg accagcgggg cggacgtttc caaggtgacc ttcttcctcg
    11521 gcatccgcca ggtttgcgag gacacgtcgg caccgttcag ctgcaagctc gtcccgaacg
    11581 gcgacgaagt aggagcccag ccgatcaagg cggtcgtcac cgacagcctc ggtcgcacgg
    11641 ccgaggacaa cgagtcaacc accgtgtcga agttcaagcc gaagggcctg agcctcagcg
    11701 cgaagcggat cggcagcaag aagcggcgga tcaaggcttc cggcaagctg atgctgccgg
    11761 cccggatgac cgcggccgac ggatgcagcg cctcgcgggt cgcaatcacc ccgaagctgg
    11821 gcaagaagtc gcttgtcaac aagcaggtca agctcgcgtc gaactgcagc tactcgctgg
    11881 cgttcggagc tccgaagaca aagaagaagc aaagggtggt gatcaaagcc agattcgccg
    11941 gcaatgactc gctctctgcc accagccaag tgagaaaggt ccggtgatca ggttgaagac
    12001 gatcgaacac gattccaacg gactgatcac agggagcaag aacatgatct ccatgcgcaa
    12061 gaaaatcact ggtgtcctcg cggccctggt tgcggcgatt gccctcacgg gtgtcttcgc
    12121 cggccaggcc caggcaatca cctacgacgc gtccacctgc acgccggaaa tctcctacga
    12181 cccggcgatc ccgacgcccc aggagttctt cggaggcccg ctggcgccca acacgggtgg
    12241 tgacaacacc tccaacaacg ccgccaagaa gaacaccacg gtgctctatc cctacctgac
    12301 ggcgctcgcg aacgcggcac ccgagatgat ccagaagcgc tccgccggta cgaccgcgct
    12361 gggcaaggag atcttcttcc tgatcatcag ctcgccggac aacatgaacg acctggagag
    12421 cgacgccgcc ttctggcgcg ccgtgcgtga gggtgagatc gccccggacg aagcgaagaa
    12481 ggccgtcttc aaccggcccg ccttcgcctg gatcgcgtcg aacgtccatg gcgacgaggc
    12541 ctccagcgcc gaagccaccg tgcggatggc ctacgaactg gctgcccgcc gtgactgcgc
    12601 caactaccag cgcctgacca acctgaccag cttcctgctg ccggttcaga acccggacgg
    12661 tcgtgacgcc tacgcgcgta ccaacgcctg ggcattcgac ctcaaccgcg actggcacac
    12721 ccaggagcag gccgagaact acctgaagat gggttcggcc ctccagtacc cgagcgtcgt
    12781 ttacgtcgac gctcaccagc agggcggcaa cagcttcttc ttcccgccga acgaggaccc
    12841 ggttcaccac gagatctcga acgccgcgat gaacgcgatc aacttcatct acggcccggc
    12901 gcttcagaag cggttcaacg accagaacat cgcctacacc aactacaaca gctacgacat
    12961 gttcacgccg gaatacggcg acacggcacc gtcgctgctg ctgggtgcgg ccggcatgac
    13021 ctacgagaag ggccgcggtc aggcctactc gaagcaggtc tatgaccact acctgtcact
    13081 ggacgagacc ctcaacacga cctcccgcct gaagcagaag atcctcggcg cctgggtcga
    13141 ccagtggtac gaggcgatcg cccagggcgc ggccggtgaa ctcgagccga accagatcgt
    13201 cagcccgagc cactcgatca cctggaacgt gccgagcgaa aaggtctacg gctacttctt
    13261 caagccgaac aaccacgacg gtgacgtcgc caagatgatt tcggtcctcc agcgggccgg
    13321 cgtcaaggtg tacaagctga actcgcggat cgccgttccc ggcattcaca tcaacggtga
    13381 cgtgaccgtg aacagcacca ccgagcttgg cccgacccgt cagaccaaga acacccagac
    13441 catggacctg ccggcgggca cgctctacat cccgatggcc cagagcatga agcactggat
    13501 ccaggccctg ctcggcgagg atcccttcgt cccctacgcg tacttctacg acgtgacggg
    13561 ctggtcgttc tcgcagctca agggactgag tggtgacggc tcgctgacca agccgatgcc
    13621 ggccgacgca agcctgagcc tgatcgccgc cccggatctc ggtggggccc cggccagcgc
    13681 gaccaagtac tacgccttca ataccgactc gtcgagggcg ctggtcatgc tgtacaagct
    13741 ggcgggcgcc aacgtcaagg tgtaccggac ggtcgccggc ttcacgctcg gtggcaagaa
    13801 gttcccgacc ggcacggcga tcctcgacgg ttcgaccgta acggcgaacg ggatcaacct
    13861 ggagacctac agcgacacct accagacgcc gatcaccggc atgtcggtgc tgccttcggt
    13921 caaccggtac ctgatcaaga agccgaagct ggcgatgctg accggaacaa ccgcggtcac
    13981 gctgccgccc gccggccgct gtgacatcgg gaccacgccc tgccaggcga tgttcgtgct
    14041 gcgcgagaag ctgggtctgc cggtcacccc ggtcacgacc acccagctcg ctgccggcga
    14101 actggtcagc ggccagtaca cggcagtcgt caacccggcc acgaccgttg cggcgggcgc
    14161 cggagccacc gcgctccaga cgttcgtcaa caacggcggc agttacttca gctggggaac
    14221 cggcggtgcc acttcggctc gtaacgccgg tatgacgaat gtaaacacga cgaccaacgg
    14281 caccaccgac ggcgttccgg tgaaggtgaa cttcaacacc aacagcccgc tgtcatgggg
    14341 cttcgacaac ggtggattcc tctaccgcgt ggggtcgggt gcggtcacct acaacccgac
    14401 cacgctgacc ggcaacggcg gctcggtccc gaacgccgtc gaggcggcct cgtacccgaa
    14461 cccgactgtc cggttctcgt acgcgaccgt cgccggcatg accaacttgt tcggcaagac
    14521 cgccgcggtt gatcaggcct tcggtgccgg ccgggtcacc ctgctctcga tcgacccgac
    14581 cttccgggcc tggctcgagg gggccgagcg cctggtcctg aacggcatcc tctacccgac
    14641 tggatcggtc atcacgccgg ctggtcacgc ggcgccggtt accgtgccgg ccggtgcgat
    14701 cgagcccggc gagggcgaga aggtcacccg caaggagctg ccgaaggtca agaaccgccc
    14761 ggcgaagaag acccacgatc cggccaatga cgtccggatc acggtgggcg tcaggcaggc
    14821 caggaacctg aagggtgcct tccgcgcggt cgctccggcc aggatcaaga agatcgccag
    14881 gttcagcaag gcgaagaacg gcaacgtcac cctgatcgtg ccgaacgccc acagcaagga
    14941 cggccacttc gccgtctggg ttcagaagct gggcgcccgg ctacaggcca acggcatcca
    15001 gccgaaggcg attcgcctct aggaagacgc cggccctaga gccggcaggc agcgagtatt
    15061 ccctcgtcca gctcgacccc caggccaaag ccattggccg gggggtcgag ttcgttaagg
    15121 gcggtcagcg ggccggtggc cgaaagcgtt tgctcgaaga gccgttcggt cgcgaggccg
    15181 tgggccatgc ccggccaggg aaggttcgga tcgagggtct gggcgacatg ggccgcggcg
    15241 gcgatgccaa ccggtccgtc cagggcgctc gagagatagg tgggcaggtg gccgccaagt
    15301 gatgcgtcga ggccgccgat cttgctcaat ttgacggtcg ccgcctcgca ggcctgctcg
    15361 aatctcgcgt cggcggcttc ttcgggatcg ttgaccgatt cgtcggcgac cagcgggatt
    15421 ccggttcccc tgctcaacgc cgcaagttca gccagtccgg tgaccggttc ctcgaccagt
    15481 tcgagcccga acggctccag cgccttcagc aggtcccctg ccgactcgcc ccaggtgccg
    15541 ttggcgtcga ttctgagctt cgcctcgtgg cccagggcgg agcggacggc cttcgccagc
    15601 tccagcgctt cacccgggcc ggccttcagt ttgaagacct cgaatccgtc ggccgcccag
    15661 gtctcgcact gagtgacaac gtcgctcact tcgccggagg tgatcgtcgc gttgcacttc
    15721 agggttcgcg gtgtggccgc cgggtcaagg tactgccaga gcggaacctg ctcgcgtttg
    15781 gcgaccaggt cggccaccgc catcgacacc gcacagcggg ccggggcaga agccgggagg
    15841 agacccgggt catccgccca ggcttccagt tcggccacca ccagctcgag ccggtcgcca
    15901 ccccggaggg acatggggac accttcgccg agcccggtca gtccgttctc gtcgcgcagc
    15961 ctgagcagca ccgattcccg ccgcgcgagc acgccggccg cggtcacata gggccggcga
    16021 aagcgcatcg cgaagggaat cacctcgacg ctgagcggac cgaccccggt gtcgttcatt
    16081 tcaggccccg agcaccagcc cgagcgcggt gaggatcgag gtcgccccga gcagggcgcc
    16141 ggtgccggcc agcgccttgt tcagcgaagg cccgtcggtg cggccagtga ccgcggcgat
    16201 cgggtcgcgg gccatcggaa tcgcaatcac gccgatcagg gccagccacg gtccgtcgtt
    16261 gaggacgatg gtcagaccga gcacgaagaa cgcggcaagt acgaggaaga catagagccg
    16321 ccgggtcttc tcgcgcccga tcttcaccgc cagcgtgttc ttgttcgccc gccggtccga
    16381 gtcgatgtca cggacgttgt tgacgaccag gatcgcggtg gatagaaacc cgatcgacac
    16441 cgaaaggccg aagggcagca gtgccagctc ctcgagctgg atgaagtagg agccgttgac
    16501 cgcgaccagg ccaaagaaca ggaagacgaa gacctcgccc agcccggcgt agccgtaagg
    16561 cctcggcccg cccgtgtaga ggaaccctgc ggcgatcgag atcaccccga ccgcgatgat
    16621 gatccagtcc gaacgccagg cgatgtagaa gccgagcagg attgcgatcg caaaggcgat
    16681 ccaggtggcg gtcagaaccc gctggggagt gatcaacccg gccgatgtga cccgcaccgg
    16741 gccgagtcga tcagccgagt cggcgccgcg ccgggcgtcc gaatagtcgt tggccaggtt
    16801 ggtgccgatc tggatcatca cgctggccgc cagagccgcc aggaagaccc cgagccgcgg
    16861 caggtcgccg gccacgtata tcgccgcggc ggtaccgacc agaaccggcg cgatcgcggc
    16921 gggaagggtg cgcggccggg ccgcgttcag ccagatcctc acggacgcct ggggaacttg
    16981 ttgaagtccg gcttgcgtcc ctcctggaag gcgtttcggc cctcctggcc ctcttcggac
    17041 atgtagaaga gcagcgtcgc atcatgggag agctgctgga ggccggtcag gccgtcctct
    17101 tcggcgttga agctggactt gagaagccgc agggagaggg gggagaggtg gagcatctcc
    17161 cggcaccagg ccacggtttc cttctcgagg tcatcgaccg gcacgaccgc gttcacgagt
    17221 cccatgtcga gggcctcttc ggcggtgtag tggcggcaga ggaaccagac ttccttggcc
    17281 cgcttgaccc cgatgttccg ggccagcagg ctggcgccga aaccgccgtc gaaggatccg
    17341 acccggggcc cggtctggcc gaaacgggcg ttgtcaccgg cgatcgtcag gtcgcagacc
    17401 agatgaagga tgtgaccacc gccgatcgcg tatccggcga ccatcgcgat gaccggtttc
    17461 ggggtgcgcc ggatctggac gtggaggtca ccgacgtcga gccggccgac gccctgctgg
    17521 gcgacttcgt catcgccgat gtagccgtca tcgccgcgaa tgcgctggtc cccgccggaa
    17581 cagaaggctt ccttgccggc cccggtgaag atgatcgccc cgacttcggt gtcgtcccgg
    17641 gctttctcga aggcgtcacg cagctccgcg aggcactggg gccggaacgc gttccggacc
    17701 tcgggccggt tgatggtgat cttcgcgata ccttcggcct tctcgtagag gatgtcggag
    17761 tattctccgg ccgaccgcca ttcgatcggc tcgtaaagcg tctcttccgg gggtgcgttg
    17821 cggtgtcttt cgggcaagtc cggtactccg ttcgtcaggc cggagccagg atggctcgcg
    17881 gcaagcaaga tttgagagca ccacgataaa cgaggtcaat tgaaactcga caactggctg
    17941 gcccagagat cgcagagctg ccccgaccgg atcgccctga tcgcggacgg cgagtcaatg
    18001 acctacgccc agctcgaaga ggaagcggtc catgcggccc ggcgccttgc cgcctgggga
    18061 gtgcgccgcg gggcgaccgt tgcgatgaac atgcggccgg gccgggacaa gatcatcctc
    18121 gtccatgccc tgatgaagac cggcgccgtg ctgctgccga tcagcccgaa gctttcaccg
    18181 gaggaacggg caaaggcaat cgagtcctgt gacgtctccg tcgacctcga cgaccccggg
    18241 aagctcaccc agaccgaagc cgacatgccg ctgctcggcg agcacgacat gtccgacccg
    18301 cattgccgca tcctgaccag cgggtcgacc ggcaggcccc ataccgtctc cctttcctac
    18361 ggcaaccatc tcttcagcgc gatgggctcc gccttcaaca tcggcgtcga cccctccgac
    18421 cgctggctgt gtgcgctgcc gaccagccac atttccgggc tctcgatcgt gatgcgctct
    18481 gtgatctacg gcaccgcgat ggtgctccag gaccacttcg aaccggaggc gatcaccgac
    18541 gcgatcgagc gcgacggggc gaccgtgatc tcgctcgtgt cgacgatgct gctcaggttg
    18601 ctcgacgagg gggtcgacct gaccgctcct cgcgcaatcc tggtcggagg cggaccggtg
    18661 ccggccagcg ccctgtcgga agctctcgac cgcggtgccc gggtgattca gacctacggg
    18721 ctgaccgaga cctgctccca ggtgaccacc ctcgaagtgg ccgaagcgcg gcgcaaggtc
    18781 ggttcggtcg gccgccccct gctcacttcg cacctgcgga tccagggtgg cgagatcctg
    18841 gttcagggtc cgaccgtttc ggccggcgcc ctcgacgccg acggctggct tcacactggt
    18901 gatgtcggct ggatcgacga ggacggcttc ctctacgttc agggccggac cgacgagatg
    18961 atcatcaccg gcggtgaaaa cgtcattccg gccgaggtcg aggacgtgct ggtccagcac
    19021 ccggagatcc tggaggcggc cgtcatcggc cggcccgacc cggaatggca gcaggcggta
    19081 acggcggtcg tggtcgcccg ggacggggtc gtggtcgacc tcgacggcgt gcggagccac
    19141 tgtgaaggca agctcgcccc gtacaagatc ccgaaggcaa tcgaactggt tcaggagttg
    19201 ccgaagaccc cctccggcaa gcttcaccgg gcagccctgc tcaagtcata gagccgcccc
    19261 gctcaagtca tggagccgct ccgggcgggt aacccatagc atcagggtca tgagcaccca
    19321 agacatcttc tacgacaagg acggcgacct ctcgaagctc gagggcaaga ccgtcgcgat
    19381 cctcggattc ggctcccagg gccacgccca tgccctgaac ctgaaggatt cgggtgtgaa
    19441 cgtcgtagtc ggccttcgcg aaggttccgc cagtcgcgag caggccgccg cggccggcct
    19501 cgaagtcctt tctgtcgccg acgcctcgag caagggcgac atcgtgatga tcctcctgcc
    19561 ggatgagaag caggccgaaa tctgggagaa cgagatcaaa gacgggattg ccgaaggcaa
    19621 cctgctgatg ttcgcccacg gtttcgcgat ccgctttgat cagatcgtgc cgccggccgg
    19681 cgttgacgtc ggcatggtcg cccccaaggg ccccggtcac atggtccgcc gtcagtttga
    19741 agaaggctcg ggtgttccct gcctgatggc ggttgagcag gacgcaaccg gaaacgccca
    19801 cgacctcgtg ctcgcctatg cgatcggcgt cggtggcggt cgcgccggga tcatcgaaac
    19861 caccttcaag gacgaatgcg aaaccgatct cttcggtgag caggccgtgc tctgcggcgg
    19921 cgccaccgaa ctggtccggg ccggtttcga cacgctggtc gaagccggtt acgacccgcg
    19981 cctggcctac ttcgagtgcc tgcacgagtt gaagctgatc gtcgacctga tgtacgagaa
    20041 ggggatccag ggcatgcgct actcgatctc caacaccgcc gagtacggtg acctgacccg
    20101 cggcaagcgg gtgatcggcg aggattcacg cgccgcgatg aaggagatcc tcggcgagat
    20161 ccagtccggc gagttcgcca aggagttcat cggcgaaaac caggccggca acgagaactt
    20221 cgaccgcatg cgccaggaag gcaaggatca caaggtcgag atcgtgggca aggagctccg
    20281 cgacatgatg ccctggatca accaggagtt ctgagcacgc gggcccagag cccaccagcc
    20341 gtccgctggc ttcgtcggcg gccggggaat gacagctcac gtgcccgcgc actatcgcgg
    20401 tcattcccgg cctgtctcct cgccagcgaa cggctggagg gctctggcct ccgcgtcccg
    20461 gcttgcctcc ttggcagcga acggctggtg gcccctgaac ttt
//
LOCUS       DAOQKN010000020        19999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_1191_length_19999_cov_5.845768, whole genome shotgun sequence.
ACCESSION   DAOQKN010000020 DAOQKN010000000
VERSION     DAOQKN010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 19999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19999
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1191_length_19999_cov_5.845768"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /locus_tag="PLJ64_02555"
     CDS             complement(<1..940)
                     /locus_tag="PLJ64_02555"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HNC92405.1"
                     /translation="MPSLLSPASPAGRLRSGVSALAERVATPLLPSDYVDLVSPLASG
                     SDLRGRIVSILPETDDAATLVIRPGRSWAGHRPGQYVRVGVDIDGVRHWRAYSLTSPP
                     DRPDGNISITVKAIEQGLVSNYLVHRVEAGTVIQLDQATGEFTFDGSTGRSALFITAG
                     SGITPVIGMLRSHTEMPDGSVLIHSAPGDREVIFRHELRRLSSTGRIELTELHTDRVG
                     MLDPVNLEAMVPDLAEREIWTCGPTGMLDSIESHLESLGLAGRLHTERFRPAVIATGE
                     GGTINFTETDLSVEGDGGTPILEVGEQAGVLMPSGCR"
     gene            1048..2226
                     /locus_tag="PLJ64_02560"
     CDS             1048..2226
                     /locus_tag="PLJ64_02560"
                     /inference="COORDINATES: protein motif:HMM:NF024945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC92406.1"
                     /translation="MSPRSSFAPESVIATLRDVLPDVAAASAGAVIAEVPEYSDLRGP
                     EGDTLDHQVELALRGFLVIASESESADSVVPFQPALDAAFALGRGEFRSGRTTDVLLS
                     AYRIGARAAWREWSKVAIGAEIPIEFFARFAEMIFAYIDQLSASSVAGYSHELEASQR
                     SRSRRFEELARRILAGGPAETLQAEAERVGWDAPEAVSAVILANARAQGIRNLVDSRS
                     LIVPGNLPGLEDEDLMLLIVPTVRTSERERIKDQLAGRGAVIGPSREWRRAGQSYARA
                     VKVLNLNRGGHRDDPADTDDFLVELVVGADPEALEDLRREVLSPLEELRPSTAEKLRT
                     TLEAWLLHQGRRDGIAAALHVHPQTVRYRMGQLREIYGDRLDDPAMVLAATVALAAKN
                     "
     gene            complement(2267..3829)
                     /locus_tag="PLJ64_02565"
     CDS             complement(2267..3829)
                     /locus_tag="PLJ64_02565"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNC92407.1"
                     /translation="MFRGEGGLRRAAAGAIVTAAALGLLAPAASAGTVRKPAGTPREL
                     RANAGGGVAANVNMTNSRKVISTGIDSGNVADLAPAWTFPLTSGGIFGAMSSNPIVSD
                     GVLYFSDAASNVYALDAFTGIQKWKRTFNVAGLGQNGVTLANGRLLAGVDSDVLSLDA
                     TTGKTIWRTTLNASDSAAVDMAPLAWKDLIIVSTIPFKIGTGIYKGGQKGVVFGLNAK
                     TGRKVWSFDTTTDNLWGNPAVNSGGGLWSTPSIDDHGTMYMSVANPGPLPGTVDFPNG
                     SSRPGRNLYTNSLVALNAATGKLKWFFQAVPHDFRDYDLQLPPILATFRVNGRKRDVV
                     ITGGKMGTVYVVDRRNGKLIWKRNVGRHNQWGDPARDLPTAAEDLPVTMYPGVAGGVE
                     TPMSVSGNSIFVPVSDLCATVVSVTTIVSCPYQTGTGAILALGGGTGRTLWKTPIPTL
                     PDGATTVVGDVVMAPGVDGTLYSLRASDGEILASPKAITGVNAPPAVSGRTVYIGAGA
                     GPGPAVQAWRLP"
     gene            3873..4913
                     /locus_tag="PLJ64_02570"
     CDS             3873..4913
                     /locus_tag="PLJ64_02570"
                     /inference="COORDINATES: protein motif:HMM:NF012417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNC92408.1"
                     /translation="MSESQHVASLTEATALFEGDPGSLRVLTSAELPVLRGISIKRLL
                     LAPGSIREPHWHANASELTYCVKGDLLVSVLDDADGFSSFTISAGEMFYVASGSLHHI
                     ENLGSGEAELILAFSHERPEDFSMHAAFGAMSDAVLGNTYDLPAADFAVISRNTEPVY
                     IVSREGRPSVPEHSSFDDPHKFAIEGQAAPIDSPAGSAKLARKQFWPIMEDLSMYSLR
                     VTPDGMREPHWHPVTAELGYVVKGEARMTVMDPDGSTDTYGLGPGDVYFIPPAYPHHI
                     EDTGQDDFHFLVFFDRHTPKDIGYRASASAISREVLAATLGIPEARLPKIPFTPEDPL
                     IVKRANPVDPPG"
     gene            complement(4915..6114)
                     /gene="nhaA"
                     /locus_tag="PLJ64_02575"
     CDS             complement(4915..6114)
                     /gene="nhaA"
                     /locus_tag="PLJ64_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HNC92409.1"
                     /translation="MKRILRQRVIDPLTDFLDDEVLGGVILLGAAIIAVVWANSGWSD
                     GYFEFWSSYIKVDIGPVDISLDLQHWINDLLMAVFFFIVGIEIKRELVSGELQNRSDA
                     VVPVLAALGGVAMPAIIFTLIAGGGPAADGWAIPAATDIAFAVGILALLGNRVSNGVR
                     VFLLTIAIVDDIVAILIIALFYGDGLSLGWAAIAGAGVLVVIGFQKFGVWRIWPYVPL
                     AALIWIGTYESGIHATIAGVVLGLLVPVRPFRGRDINTKLEHALHPVSAFVVVPLFAL
                     ANAGIDLRGGVLGDALGSELTWAVIAGLVVGKILGISVTTLAALRFGSGTLPEGMRRA
                     QVWGVGALGGIGFTVSLFITDLAFTDPLLLDAAKVGIFLGSILSGLLGTILLLRRRGA
                     GPPAETD"
     gene            6164..7057
                     /locus_tag="PLJ64_02580"
     CDS             6164..7057
                     /locus_tag="PLJ64_02580"
                     /inference="COORDINATES: protein motif:HMM:NF016013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HNC92410.1"
                     /translation="MLRLRVDVLQSERDQLVERLTCLDGVRRITSEPELHGGQFMISA
                     DVEPAAADDVLSALHTLDLHHDDFVLVREDILTQGLRSGGLSASGGYSWVEILGEARA
                     NARPVARYFLLMAVAGWIAGLGVIQSSTILIVGAMAVSPDLMPICSTCVGIIGRRLNL
                     AWSSFLTLILGMGCVMLVSFIVALALQLVGILASDFDVREIELEGLAAADYSTGLIAL
                     GAGVAAMLSFETRGASAVGVAISVTTVPASAYFGVAMALGEASEALGALLVLGINLVL
                     LIVGGSITLYFQRLFTPIHET"
     gene            7129..7788
                     /locus_tag="PLJ64_02585"
     CDS             7129..7788
                     /locus_tag="PLJ64_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC92411.1"
                     /translation="MASPSRTRLSREDRMEQTLDTAHSLFAERGYAAVTMDEIAAAVG
                     VTKPLIYNYFGNKESLYIACMERSGDALVEAISLAVADAADPSQAFERGVRAFFSFID
                     TDRAAWAVLFDETLPHGGEVIERVSYYRSQLLDFVAASTLSRMPAKARQSESVRTEVE
                     AVATAVIGAAEAMGRWWLAGGSMTASEAAELLISTVEPGLLARSAPNTPKAVTEGDPP
                     A"
     gene            7785..9077
                     /locus_tag="PLJ64_02590"
     CDS             7785..9077
                     /locus_tag="PLJ64_02590"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HNC92412.1"
                     /translation="MNKKTRKVAVVGANRIPFARSNGAYSHASNQDMLTSALDGLIDR
                     FGLEGETLGEVAGGAVLKHSRDRDLTRESVLGTRLAAETPAYDVQQACGTGLETTVLV
                     ANKIALGQIDAGIACGADTTSDAPIALNEELRETLLEANRQTTTLGRIKALTNVRPQH
                     LVPAIPRNEEPRTGLSMGEHCAIMAADWEISRSEQDALTVASHHNLAAAYERGFFEDL
                     ITPYLGLRQDQNLRPDSSLEKLAGLKPVFGGPEGTMTAANSTPLSDGASAVLLASEDW
                     AKERGLPILAFVEEAQTAAVDYLHKREGLLMAPAYAMSEMLARANLSLQDFDYYEIHE
                     AFAAQVLCTLKAWEDPDYCRQKLGRSEAMGPIDRSKLNVNGGSLAAAHPFAATGGRIV
                     GSLAKQLHEDGTGRGVISICAAGGQGVVAILEAPGGKK"
     gene            9074..10399
                     /locus_tag="PLJ64_02595"
     CDS             9074..10399
                     /locus_tag="PLJ64_02595"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HNC92413.1"
                     /translation="MSDTYTQIVNAPVVSGLANSLGLPRPVELDRYQPGRPVIDGPVL
                     TGSAPESSLRPAISATLDSIGAERAGTEGPAKALIFDATGISDSTELVELQRFFYPAV
                     PRLAPSGRAIVLGTPPAATGGVRAHTAQRALEGFVRSLGKEIGGRGSTAQLVQVEPGA
                     GDEIESTLRFLLSPRSAFVSGQVIRVGKPVAPKPELDWERPLAGRTALVTGASRGIGE
                     AIATVLARDGATVIGLDIPPAAAELDQVTSRLGGRSLTLDITTPDAPDAIARELGEGV
                     DLIVHNAGVTLDRTIAKMPEERWSKLMEINLSSEERINDAILSADLLNANGRIVCVSS
                     MSGIAGNSGQTNYAASKAGVIGMVESMAPELARRQATINAVAPGFIETQMTAAMPFGT
                     REVGRRLNSLSQGGKPVDVAETIAWFASPASTGVNGNVVRVCGQNLLGA"
     gene            10399..11307
                     /locus_tag="PLJ64_02600"
     CDS             10399..11307
                     /locus_tag="PLJ64_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HNC92414.1"
                     /translation="MTRLESSPSILPLYLKAAMPLIPGASSLPFLPGGGGEIPSGLGF
                     ELKGVVPQQDQVAEYAKVCGFPLKDRLPPTFPHVLAFPLHMAVMSNGEFPFGAVGLVH
                     IENSINQLRPIAFGEALEVKVGVTSLSPHPKGQIFSLLTEVSAAGETVWTETSTMLRR
                     GKGSQTPSAPGLEIPTLDPGTPASATWKLPADLGRRYGAVSGDRNPIHMHALTAKPLG
                     FHSAIAHGMWTAARALAQIESRLPDSFTAGVRFRKPIFLPARVQFAAEGDGTETLFAV
                     RDARKGTPHMDGRITSNPIETAKANN"
     gene            11309..12652
                     /locus_tag="PLJ64_02605"
     CDS             11309..12652
                     /locus_tag="PLJ64_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNC92415.1"
                     /translation="MSSKNSQGLADKTMGSSLRALNWIAGSKILDRLRLRSQFERTVF
                     QSTKHGMRAATSAGRSFNKATSLGKPARQKSGSGSGLFDLTPDDEQQMFIEAGQAFAA
                     DQIRPAASGADSEKATPAAILEQAGEIGVNTLGIPEEFDGVLTEQSTVTWTLMAEALA
                     HGDMGIAYAALAPGAAATAIGRWGDPDQQASYLPAFTGENPPAAALAILEPKPLFDPF
                     NPDTRATAGTGGHWILNGVKSMVARPGECELFIVAAATGDARPTLFIIESGSEGLTFE
                     AEPAMGLRAASTGRLVLEDVRVPASAMLGEEGDRARIYADCVSGSRIAWSAMFSGTAQ
                     AVLDYVIPYVNERQAFGEPISNRQSVAFSVSDIAIETGGMRLANWRAASRADRGESFA
                     REAALARRLAAAKGVEIGSAGVQLLGGHGYVKEHPVERWYRDLRTAGVMEGALLA"
     gene            12678..13895
                     /locus_tag="PLJ64_02610"
     CDS             12678..13895
                     /locus_tag="PLJ64_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNC92416.1"
                     /translation="MIYLETPKKFRPLVTQANHVAREIFRPISRKYDEAEHEYPKELD
                     VLASLINGMNEGSGMGAGAAGVSGESGDEPDSGSGNRNGSNLSTVLGIIELCWGDVGL
                     LLAMPGQGLGNSAIASVATAEQLERYGGKWAAMAITEPEAGSDSAAIRTTAVLDGDEY
                     VLNGEKIFVTSGDRAELIVVWASLDRSVGRAAIKSFIVERDNPGLTLDRLEHKLGIRA
                     SDTANFTLQDCRVPKEALLGSPEVDVKKGFAGVMKTFDNTRPLVAGMALGVTRAALQE
                     TKRQLEAAGVEIDYDTPALCQSAAAARYLEMEADYEAALMLTLQAAWMADNRKPNSLE
                     ASMAKAKAGRTGVDVTLGCVEICGSIGLGESELLEKWSRDAKILDIFEGTQQIQLLII
                     ARQLLDKTSAELK"
     gene            complement(13911..15605)
                     /locus_tag="PLJ64_02615"
     CDS             complement(13911..15605)
                     /locus_tag="PLJ64_02615"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF005127.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNC92417.1"
                     /translation="MIEIIETPIADLRAALQAGEITAVELVEAFYARIDTFDGPDTET
                     RLNSVVVRNAEAMAEAAASDARRANGQAMGPLDGIPYTAKDSYLVRGMTAASGSPAFV
                     NLLAMRDAFTVKRLREGGAICLGKTNMPPMANGGMQRGVYGRAESPYNADYLTAPFAS
                     GSSNGAGTATAASFAAFGLAEETWSSGRGPASNNGLCAYTPSRGVISVRGNWPLTPTM
                     DVVVPYARTMADLLEVLDVIVADDPDTRGDLWRAQEWIELPAASEVRPDSYPSLAASS
                     EALAGNRFGIPRMYINADPAAGTGEKSDVWGPTGERIDTRQSVIDLWQAARNDLEAAG
                     AEVVEVDFPVVSNYEGDRAGCPNIFTRGLVSPAFLDEELRGLSAWAYDDFLRANDDPA
                     LNRLADADGELIFPPEAGALPSREGDLAFGMEEYVEMAWAGLTPWDQLPELEAGLRGL
                     EQTRRIDLEEWMDELGLDTVVFPAVADVGPADADVNPRSADRAWANGVWVANGNLVPR
                     HLGIPTVTVPMGLMGDIGMPVGLTFAGRSYDDNRLLTQASAFESIRPRRIPPPRTT"
     gene            complement(15654..16121)
                     /locus_tag="PLJ64_02620"
     CDS             complement(15654..16121)
                     /locus_tag="PLJ64_02620"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNC92418.1"
                     /translation="MAPEDPVELSGNRLEVVRAFKNAYRTLSRRKGRETHRLGATEVS
                     QSQFELLVELHKSGPLAVGELAQTNGLSPASVSQMVDRLSDQGHVERIRSEEDRRIVK
                     VALSDQGRSVIEPIIESWHRKWNEALEGIPDRDLEAASGVLDRIAGIYEDDGS"
     gene            complement(16111..17565)
                     /locus_tag="PLJ64_02625"
     CDS             complement(16111..17565)
                     /locus_tag="PLJ64_02625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HNC92419.1"
                     /translation="MNQDSTTRIEPYVWKVAGVVILGMIMSILDTTIVNVALDTLAKD
                     LHTTISEIQWVATGYLLSLAAVIPLTGWAARKFGAKNVYIWSIVLFTLGSALCGIAAS
                     STSLIAFRVIQGIGGGMIMPIAQMIMAEVAGPKRMGKVMSVTVMPAMLAPILGPTIGG
                     LILQNFHWSWIFFVNVPVGIVAALLAVRMLPHSKLGEPGKLDWAGLVLMVLGIPSLVF
                     GISEVGTTGTFNSPEVIWPVIAGLVLIGAFIRHAYRAERPLLDVRLYANRVFGAASLT
                     TFALGAALFGAMILMPLYYQQIRGESVIATGLLLAPQGLGMLMVGPFAPRLTERFGGG
                     KVATFGVAVLSLGSLPFVFIGVDTSIAFLSLMLLVRGIGIGLAFMPTMTVAYASLRKD
                     QLSDATPQMNVIQRVGGAIGTAVLAVVLQQASEGATTATAAAQGFGTAYLWALGIALL
                     ALIPCVVLWRAEMPAGPVTEEERIAEVEIEPLGA"
     gene            complement(17616..19259)
                     /locus_tag="PLJ64_02630"
     CDS             complement(17616..19259)
                     /locus_tag="PLJ64_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92420.1"
                     /translation="MNSEEIREPGARRGWLALAAMAAMLLMAAPAARAAECPDVINAS
                     AFASKARLHQMTAKFNSFGPRILGSPAHNQAIAWLEGQARSIGLRVHSTYFHPYTWLP
                     RTRMKKGPGLDIGAAGAIRVRKQDGSILEVADAGAVHWSKPTSSKGEGGPLVYLPADQ
                     QITAENAAGKVVIRDFRIGSIPFGVVGSLLGLYQTPDLEGTTDYARPYLSPNIHADQL
                     DASAAGANGVIFVFDLPASQIRGYYDPHVGMIYRQPAVFVGRSQGERLKELAGRGTSA
                     RVVVNANLARRKTRNLIARLPGQSNEKMVMVANTDGTSWVQENGVIGMLAFARYYAKL
                     PLRCRPRTLELVFSSAHDSYRNDGMSPRHYPLDKKRIAFGFAIEHLGTREVIPTGEGT
                     SRHLAFTGVADPALFGAGDSDALREAAVEATKRRNLPRTAVLKGLSVPNPDQAPSICS
                     MGGLGNSFQPELVPTLAMISGPWSLYDPVFGEKAIDFGQMRKQVLAAGDTVLALDGLP
                     RAEIAGEYPALQAELDAGTKTPCPETDVFPQRAPGPGGV"
     gene            19430..>19999
                     /locus_tag="PLJ64_02635"
     CDS             19430..>19999
                     /locus_tag="PLJ64_02635"
                     /inference="COORDINATES: protein motif:HMM:NF023834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNC92421.1"
                     /translation="MGHYKSNVRDIEFNLFEMFDTDGVLKTGAFGDLDSDTVRMMLEE
                     ASKLAEGPVAAAFESSDRTPPSFDADTHDVSLPADFKASVNAWTEADWEKIGLIEEVG
                     GIPAPATVSWAINEFMCGALPAGFFYLAGPAFASLLYKNGNEEQKHWAQMAVEKNWGA
                     TMVLTEPDAGSDVGAGRTKAIDQGDGTWHI"
BASE COUNT         3485 a         6792 c         6438 g         3284 t
ORIGIN      
        1 tccggcagcc ggagggcatc agcaccccgg cctgctcgcc gacttcgaga atcggcgttc
       61 cgccgtcccc ttcgacgctc aggtctgtct cggtgaagtt gatggtgccg ccctcccccg
      121 tggcgatcac cgcggggcgg aaacgctcgg tgtgaagacg gccggccagc cccagcgatt
      181 caaggtgact ctcgatcgaa tcgagcatcc cggtcggtcc gcaagtccag atctcccgct
      241 cggcgaggtc cggcaccatc gcttccagat tgaccgggtc aagcatgccg acccggtccg
      301 tgtgaagttc cgtcagctcg atccggccgg ttgaggagag ccgtctcagt tcgtgcctga
      361 agatcacctc ccggtcgccc ggagcggaat ggatgagcac cgatccgtcg ggcatttcgg
      421 tgtggctgcg gagcatcccg atcaccgggg taatgccgct gcccgcggtg atgaagagag
      481 cgctgcgccc ggtgctcccg tcgaaggtga actcgccggt cgcctgatcg agctgaatta
      541 cggttccggc ctcaacccga tgcacgaggt agttgctgac caggccctgc tcgatcgcct
      601 tgaccgtgat cgagatgttg ccgtcgggcc ggtccggcgg cgaagtcagg gaataggccc
      661 gccagtgccg gactccgtct atgtccaccc cgacccgcac gtactggcca ggtcggtgcc
      721 cggcccagct gcgtccgggc cggatcacga gcgtggctgc gtcgtccgtc tccggcaaga
      781 tcgagacgat ccgaccgcgc agatcggaac ctgacgcgag cggcgaaacc aggtcgacgt
      841 aatcggacgg caggagtggc gtcgccactc gctccgccag ggcgctcacc ccggaacgga
      901 gccttccggc ggggctggcg ggtgaaagca gagaaggcat gacccaagag tggactatcc
      961 gaggagaagt atcttgctcc tgccggttga atcggaggct cgcattttat tgtttgcgaa
     1021 caatccatgc agctatgatt gccgtcaatg agccctcgat cctccttcgc acccgaatcg
     1081 gtgatcgcta ccctgcggga cgtcctgccg gatgtcgcag ccgcctcggc tggcgcggtc
     1141 atcgctgaag ttcccgaata cagcgacctg cgaggacccg aaggcgacac cctcgaccat
     1201 caggttgaac ttgcgctgag gggctttctg gtcatcgcct cggaatccga atcagccgat
     1261 tccgtggttc cgttccagcc tgcccttgac gccgcctttg cccttggtcg aggcgagttc
     1321 cggagcggcc gaactaccga cgtgctcctc tccgcgtacc ggatcggggc cagggcggcc
     1381 tggcgggaat ggtcaaaggt ggcgatcggg gctgaaatcc cgatcgagtt cttcgcccga
     1441 ttcgccgaga tgatcttcgc ctacatcgac cagctttctg cttccagcgt ggccggctac
     1501 tcccacgagc tggaagccag ccagcggtcc cggagccggc gcttcgagga gcttgcccgc
     1561 aggatcctgg ctggcggacc ggccgagacc cttcaggccg aggcggaaag ggtcggctgg
     1621 gatgccccgg aagcggtgtc cgccgtgatc ctcgccaacg ccagggccca ggggatccgg
     1681 aacctggtcg attcgcgcag cctgatcgtt cccggcaacc tgcccggcct cgaggacgag
     1741 gacctcatgc tgctgatcgt tcccacggtc cggaccagcg aacgcgagcg catcaaggat
     1801 cagctggccg gccggggtgc ggtgatcgga ccgtcccgtg agtggcggcg ggccggtcag
     1861 tcctacgccc gggcggtcaa ggtgctcaac ctgaaccgcg gcgggcaccg ggatgacccg
     1921 gccgacaccg acgacttcct ggtcgaactg gtggtcgggg ccgaccccga agcccttgag
     1981 gacctcagaa gggaagtcct ctcccccctt gaggaacttc gtccctcgac cgcagagaag
     2041 ctccggacca cgcttgaagc ctggctgctc caccagggtc gccgcgacgg tatcgccgcc
     2101 gcccttcacg ttcatccgca gacggttcgc taccggatgg ggcagctgag ggaaatctac
     2161 ggcgaccggc tggacgatcc cgccatggtc ctcgccgcca cggttgccct ggccgccaaa
     2221 aattgaagtc ccggggtcga agtgcccggc cggccgatcg ccccgctcag ggcagtcgcc
     2281 aggcctggac cgccgggccg gggcccgctc cggcaccgat gtagacggtt cggccggaaa
     2341 ctgccggcgg tgcgttgacg ccggtgatcg ccttcggaga ggcgaggatt tcaccgtcac
     2401 tggcccgcag cgagtaaagg gtcccgtcca ctcccggggc catgacgaca tcacccacca
     2461 ccgtcgtcgc tccgtccggc agggtcggga tcggggtctt ccagagcgtc cttccggtgc
     2521 cgccaccgag ggccaggatc gctccggtcc cggtctggta gggacaggag acgatggtcg
     2581 tcaccgagac gaccgtcgcg cagaggtcgg acaccggcac gaagatggaa ttgccggaaa
     2641 ccgacatcgg ggtttcgacg ccgccggcga cccccggata catggtgacc ggcagatcct
     2701 ccgccgcggt cggaaggtcc ctggccgggt cgccccactg gttgtgcctg ccgacgtttc
     2761 gcttccagat cagctttccg ttccggcggt caaccacgta gacggttccc atcttgccgc
     2821 cggtgatgac cacatcccgt ttccggccgt tcaccctgaa ggtggcgaga atcggaggca
     2881 gttgcaggtc gtagtcccgg aagtcatgcg gaacggcctg gaagaaccat ttgagtttgc
     2941 cggtcgccgc gttcagcgcg accaggctgt tcgtgtagag attcctgccg ggccggctcg
     3001 aaccgttggg gaaatcgacc gtccccggca gcggccccgg attggcgacg ctcatgtaca
     3061 tcgttccgtg gtcatcgatg ctgggggtgc tccagagccc tcccccgctg ttgaccgcgg
     3121 ggttgcccca gagattgtcg gtcgtcgtgt cgaaagacca gaccttgcga ccagttttcg
     3181 cgttgagccc gaagacgacg cccttctgcc cgcctttgta gatcccggtc ccgatcttga
     3241 acgggatcgt cgagacgatg atcaggtcct tccaggcgag cggggccatg tccacggcgg
     3301 cgctgtcact cgcgttcagg gtggtgcgcc aaatggtttt gccggtcgtc gcgtcaaggc
     3361 tcagcacatc ggaatcgaca ccggccagca ggcgtccgtt cgcgagggtc acaccgttct
     3421 ggcccagtcc ggccacgttg aaggtgcgtt tccatttctg gatcccggtg aaggcgtcca
     3481 gcgcatagac gttcgatgcc gcgtccgaga agtagaggac cccgtcgctg acgatcgggt
     3541 tcgaagacat cgcgccgaag atgccgccgg aagtgagcgg gaaggtccag gccggggcaa
     3601 ggtccgccac gttgcccgag tcgatcccgg tcgagatcac cttccgggag ttggtcatgt
     3661 tgacgttcgc ggcgaccccg ccaccggcat tggcccgcaa ctcgcgcggc gtcccggccg
     3721 gtttccggac cgtgccggcg gacgccgccg gggcgagcag gcccagggcg gcggcagtga
     3781 cgatcgcgcc agcggcagct ctgcgaagac ccccctctcc ccgaaacatc cttcgatcct
     3841 acccccgatc cccgctgcta gggtcgccgg acatgagcga gagtcagcat gttgcttccc
     3901 tgaccgaagc cacggccctg ttcgagggcg acccgggctc gctccgggtt ctgacttcgg
     3961 ccgagctgcc ggttttacgc gggatctcga tcaagcggct gctcctcgcc ccgggttcga
     4021 tccgggaacc gcactggcac gccaacgcca gcgaattgac ctactgcgtc aagggagacc
     4081 tgctggtctc ggtcctcgac gacgccgacg gcttctcctc gttcacgatt tcggccgggg
     4141 agatgttcta cgtggccagc ggcagcctgc accacatcga gaacctgggt tccggcgagg
     4201 ccgagttgat cctggcgttc agtcacgagc gacccgaaga cttttcgatg cacgccgcct
     4261 tcggagcgat gagtgacgcc gtactcggca acacctacga cctgcctgcc gcggatttcg
     4321 ccgtgatttc ccgcaatacc gagccggttt acatcgtcag ccgcgagggc cggccgtcgg
     4381 ttccggagca ttcctcattc gacgatccac acaagttcgc gatcgaaggg caggctgccc
     4441 cgatcgactc cccggccggc tcggcgaagc tggcccgcaa gcagttctgg ccgatcatgg
     4501 aggacctctc gatgtactcg ctgcgggtca ccccggacgg aatgagggaa ccgcattggc
     4561 atccggtcac cgccgagctg gggtacgtgg tcaagggcga ggccaggatg acggtgatgg
     4621 atccggacgg ttcgaccgat acctacgggc tcgggcccgg cgatgtctac ttcatccctc
     4681 ccgcctaccc tcaccacatc gaggacaccg gtcaggacga cttccacttc ctcgttttct
     4741 ttgaccggca caccccgaag gacatcggct accgggcttc ggcttcggcc atttcgcggg
     4801 aggtgctggc cgcgacgctg gggatccccg aggccaggct gccgaagatc cccttcaccc
     4861 cggaagatcc gctgatcgtg aagcgggcca atccggtcga cccgcccggc tgagtcagtc
     4921 cgtttcggcc gggggcccgg ccccgcgccg gcgcaggaga agaatcgtgc ccaggaggcc
     4981 gctgaggatc gagccgagga agatgccgac cttggccgcg tcgagcagga gtggatccgt
     5041 aaaggccaga tcggtgatga acagcgaaac ggtgaagccg atgccaccga gcgcgcccac
     5101 gccccagacc tgtgctctcc gcatgccttc cggcagggtg ccggaaccga aacgcaaggc
     5161 agccagggtc gtgaccgaga tcccgaggat cttgccgacc accaggccgg cgatcaccgc
     5221 ccaggtcagc tcgctgccga gcgcatcacc cagaaccccg cccctcaggt cgatgcccgc
     5281 attggcgagg gcgaagagcg gcaccaccac gaaggcgctg accgggtgaa gggcatgctc
     5341 gagctttgtg ttgatgtccc tgccgcggaa aggcctgacc ggcaccagca gccccagcac
     5401 gacgccggcg atcgtcgcgt ggattcccga ctcataggtg ccgatccaga tcagggcggc
     5461 caacggcacg tagggccaga ttcgccagac gccgaacttc tggaatccga tcacgaccag
     5521 gaccccggcc ccggcaatcg ccgcccagcc gagcgaaagc ccgtctccgt aaaagagggc
     5581 gatgatcagg atcgcgacga tgtcatcgac gatcgcaatg gtcagcagga aaacccggac
     5641 cccgttcgaa acccggtttc cgagcaaggc gaggatcccg accgcgaagg cgatgtcggt
     5701 cgccgccggg atcgcccagc cgtccgccgc cgggccgccg ccggcgatca gggtgaagat
     5761 gatcgccggc attgccaccc cgccgagcgc cgccagtacc ggcacgaccg cgtcactgcg
     5821 gttctggagc tcgccggaaa cgagctcccg ctttatctcg attccgacga tgaagaagaa
     5881 gaccgccatc agcaggtcgt tgatccagtg ctgaagatcc aggctgatgt caaccgggcc
     5941 gatgtcgacc ttgatgtaac tggaccagaa ctcgaagtac ccgtcgctcc agcccgaatt
     6001 ggcccagacc acggcgatga tcgccgcgcc cagcaggatg acgccaccga gtacctcgtc
     6061 gtcgaggaag tcggtcagcg ggtcgatcac tcgttgtctg agaatgcgct tcatatccgt
     6121 cctgctgcat gggtactttg ccggaagtgg aagatgggtt gccatgctga ggctgcgggt
     6181 ggacgtgctc cagagcgagc gcgaccaact agtcgagagg ctcacctgcc tcgacggggt
     6241 gaggcgcatc acctccgaac ccgaactcca cggcggtcag ttcatgatct cggccgatgt
     6301 tgaaccggca gccgccgacg acgttctgag tgccctgcac acccttgacc tgcaccacga
     6361 cgatttcgtg ctggtccggg aggacatcct cacccagggc ctccgctctg gcggactctc
     6421 ggccagcggc ggctactcct gggtcgagat tctcggtgaa gcgagggcca acgcccgacc
     6481 ggtggcccga tacttcctgc tcatggcggt ggccggctgg atcgccgggc tcggcgtgat
     6541 ccagtccagc acgatcctga tcgtcggcgc gatggcggtc agtcccgatc tgatgccgat
     6601 ctgctcgacc tgcgtcggga tcatcggccg gcggctgaac ctggcctgga gttccttcct
     6661 cactctgatc ctcggcatgg gctgcgtgat gctggtctcc ttcatcgttg ccctcgctct
     6721 ccagcttgta ggcatcctcg ccagcgattt cgacgtgcgc gagatcgagc tggaagggct
     6781 ggccgccgcc gactactcaa ccgggctgat cgccctcggg gccggagtcg cggccatgct
     6841 ctccttcgaa acccgcggcg ccagcgcggt cggagtcgcc atttcggtga ccaccgttcc
     6901 cgcctcggcc tacttcgggg tggcgatggc cctgggcgag gcaagcgagg ccctcggtgc
     6961 cctgctcgta ctcggcatca acctcgtgct gctgatcgtc ggcggctcga taacgctcta
     7021 tttccagcga cttttcactc caattcacga gacgtgaaca gccgtcgcct gtgtgcgatc
     7081 ggtcacgtta ccgatatata aatttatatc cgggtaactt ggtttgcgat ggcttccccc
     7141 tcccgcacca ggctctcccg cgaggaccgc atggaacaga cgctggatac agctcactcg
     7201 ctgttcgccg aacgcggtta cgccgccgtg acgatggacg aaatcgccgc ggcggtcggt
     7261 gtgaccaagc cgctgatcta caactacttc ggcaacaagg aaagcctcta catcgcctgc
     7321 atggaacggt ccggcgatgc cctggtcgag gcgatttccc tggccgtcgc cgatgccgcc
     7381 gacccgtcgc aggccttcga gcgcggggtc cgcgccttct tctcgtttat tgacaccgac
     7441 cgcgccgcct gggcagtcct gttcgacgag acgctccccc acggcggcga agtgatcgag
     7501 cgcgtgtcct actaccggag ccagctgctc gacttcgtgg ccgcctcgac cctgagccgg
     7561 atgcctgcga aggcccggca aagcgaaagc gtcagaaccg aagtcgaagc cgtcgccacc
     7621 gcggtgatcg gagcggctga agcgatgggc cgctggtggc ttgccggcgg ctcgatgacc
     7681 gcatccgaag cggccgaact tctcatttcc acggtcgagc cgggcctgct tgcccggagc
     7741 gcacccaaca cgccaaaggc agtaaccgaa ggagacccgc ccgcatgaac aagaagaccc
     7801 gcaaggtcgc tgtcgtaggc gccaaccgga tcccgttcgc gagatccaac ggggcctact
     7861 cgcatgcctc caaccaggac atgctgactt ccgccctcga cggcctgatc gaccgcttcg
     7921 gcctcgaggg cgagaccctc ggtgaagtgg ccggcggcgc cgttctgaag cattcgcgtg
     7981 accgcgacct gacccgcgag agcgtgctcg gcacccggct ggcggccgag acccccgcct
     8041 atgacgttca gcaggcctgc ggcaccggcc ttgaaacgac cgtcctggtc gccaacaaga
     8101 tcgcgctcgg gcagatcgac gccggaatcg cctgcggggc cgacaccaca tccgacgccc
     8161 cgatagccct caacgaggag cttcgcgaga ccctgctgga agccaaccgg cagacgacga
     8221 ccctgggccg gatcaaggcc ctgaccaatg tccggcccca gcacctggtc cccgcgattc
     8281 cccgcaacga ggaaccgcgc accggccttt cgatgggcga gcactgcgcg atcatggcgg
     8341 ccgactggga aatcagccga agcgaacagg acgcgctgac cgtggccagc caccacaacc
     8401 tcgcggccgc ctatgaacgc ggcttcttcg aggacctgat cactccctac ctcggcctgc
     8461 gacaggacca gaacctgcgg cccgactcct cgctcgagaa gctggccggg ctgaagccgg
     8521 tcttcggcgg gccggagggc acgatgaccg cggccaactc gaccccgctc tcggacggtg
     8581 cctcggccgt gctgctggct tcagaggact gggccaaaga gcgcggcctg ccaatcctcg
     8641 ccttcgtcga ggaagcccag accgccgcgg tcgattacct ccacaagcgg gagggcctct
     8701 tgatggcccc ggcctacgcg atgtcggaga tgctggcccg ggccaacctc tcgcttcagg
     8761 atttcgacta ctacgagatc cacgaagcct tcgccgccca ggtgctctgc accctcaagg
     8821 cctgggaaga ccccgactac tgtcgccaga aactcggtcg cagcgaggcg atggggccga
     8881 tcgaccggtc gaagctgaac gtgaacggcg gctcgctggc tgccgcccac cctttcgctg
     8941 cgaccggcgg ccggatcgtc ggatcgctgg ccaagcaact gcatgaagac ggcaccggcc
     9001 gtggcgtgat cagcatctgc gccgccggcg ggcagggtgt cgtggcaatt ctcgaagcgc
     9061 ctggaggcaa gaagtgagcg acacctacac acagatcgtc aacgcaccgg tcgtttcggg
     9121 cctggccaac tcactgggtt tgccgcggcc ggtcgagctt gaccgctacc agccgggccg
     9181 accggtcatc gacgggccgg tactgaccgg gtccgctccg gagagcagcc tgcgccccgc
     9241 gatcagcgcc accctcgact cgattggcgc cgaacgggcg ggaaccgaag gtccggcgaa
     9301 ggcactcatc ttcgatgcca ccggaatcag cgactcgacc gagctggtcg aactgcagcg
     9361 cttcttctac cccgcggtgc cacggctcgc accgagcggc cgggccatcg tgctcggcac
     9421 accacccgcc gcgaccggcg gcgtccgcgc ccacaccgcc cagcgggcac ttgaagggtt
     9481 cgtccgctcg ctcggcaagg agatcggtgg ccgtggttcc accgcccagt tggtgcaggt
     9541 cgagcccggc gccggggacg aaatcgagtc caccctgcgc ttcctgcttt ccccccgttc
     9601 ggctttcgtt tccgggcagg tgatccgggt cggcaagccg gtcgctccga agccggaact
     9661 cgactgggaa cggcccctgg ccggaaggac tgcgctggtc accggggctt cacggggaat
     9721 cggagaggcg atcgccaccg tcctcgcgcg ggacggggcg accgtcatcg gactcgacat
     9781 tccgcccgcc gcggccgagc tggaccaggt gacttcgcgc ctcggcgggc gttccctgac
     9841 cctggacatc acgacccccg atgcgcctga cgcgattgcc cgcgagctcg gcgaaggagt
     9901 cgacctgatc gtccacaacg ccggcgtcac tctggaccgc acgatcgcca agatgccgga
     9961 agagcgctgg agcaagctga tggaaatcaa cctctccagc gaggaacgga tcaatgacgc
    10021 gatcctttcc gccgacctgc tgaacgccaa cggccggatc gtctgcgttt catcgatgtc
    10081 cggcatcgcc ggcaattcag gccagaccaa ttacgccgct tccaaggccg gtgtgatcgg
    10141 catggtcgaa tcgatggcac cagaactggc gcggcgacag gccacgatca acgcggtcgc
    10201 ccccggattc atcgagacgc agatgaccgc ggcgatgcca ttcggcaccc gtgaggtcgg
    10261 ccgccgcctg aacagcctct cgcaaggtgg caagccggtt gacgtggcgg agacgatcgc
    10321 ctggttcgcc agccccgcct caaccggggt caacggaaac gtggttaggg tctgcggcca
    10381 gaacctgctg ggagcctgat gaccaggctc gaatcctccc cgtcgattct gccgctctat
    10441 ctgaaggcgg cgatgccgct gattcccggc gcctcctccc tgcccttcct gcctggcggc
    10501 ggcggggaga ttccttccgg gctcggcttc gaactgaagg gagtggttcc gcagcaggac
    10561 caggtagccg aatacgccaa ggtctgcggg ttcccgttga aggacaggct gccgccaacc
    10621 ttcccccacg tgctggcctt tccgcttcac atggcggtga tgagcaacgg ggagtttccc
    10681 ttcggcgcgg tcggcctggt tcacatcgaa aactcgatca accaactcag gccgatcgca
    10741 ttcggcgaag cgcttgaagt gaaagtcgga gtcacttcac tctcccccca cccgaaggga
    10801 cagatctttt ccctgttgac cgaggtcagt gccgccgggg agacggtctg gaccgagacg
    10861 agcaccatgc tgcggcgcgg gaagggcagc cagacgccct ccgctccggg cctcgaaatc
    10921 cccacgcttg accccgggac tccggccagc gccacctgga aactcccggc cgacctcggc
    10981 cggcgctacg gcgcggtctc cggggatcgc aacccgatcc acatgcatgc cctgaccgca
    11041 aagccactgg gcttccactc cgcgatcgct cacggaatgt ggaccgccgc ccgggccctg
    11101 gcccagatcg agagccggct tcccgattcc ttcactgccg gggtcaggtt ccgcaagccg
    11161 atcttcctgc ccgcccgggt tcagttcgcc gccgaaggcg acggcacgga aaccctcttt
    11221 gccgtccgcg atgcccgcaa gggcacccct cacatggacg gtcgcatcac ttccaatcca
    11281 atcgaaaccg cgaaagcgaa caattgaaat gagcagcaag aacagccagg gactggccga
    11341 caagacgatg ggctccagcc ttcgtgccct gaactggatc gccggatcga agatcctcga
    11401 ccggctcagg ctgcgcagcc agttcgagcg gaccgtcttt cagagcacta aacacggaat
    11461 gcgcgccgcg accagcgccg gccgcagctt caacaaggcg accagcctcg gcaagccggc
    11521 ccgccagaag agcggctccg gatccgggct cttcgacctc actccggatg acgaacagca
    11581 gatgttcatt gaggccgggc aggcctttgc ggccgaccag atccggcccg ccgcctccgg
    11641 ggcggactcg gagaaggcca ctccggccgc gatccttgag caggccggcg agatcggcgt
    11701 caacacactt ggcatccccg aggaatttga cggggtcctg accgagcagt cgaccgtaac
    11761 ctggaccctg atggccgagg ccctcgccca cggtgacatg ggcatcgcct atgcggcgct
    11821 ggcccccggc gcggcagcaa ccgcgatcgg ccgctggggc gaccccgacc agcaggcgag
    11881 ctacctgccc gccttcaccg gcgaaaatcc gccggccgcc gcgctggcga tcctcgagcc
    11941 gaagccgctc tttgatccct tcaatcccga cacccgggcc accgccggaa ccggcggcca
    12001 ctggatcctc aacggggtga agtcgatggt cgcccggccc ggcgagtgcg agctcttcat
    12061 cgtcgcggcc gccaccggtg acgccaggcc gacgctcttc atcatcgaat ccggctccga
    12121 gggcctgacc ttcgaagccg aacccgcgat ggggctcagg gccgcatcga ccggccggct
    12181 ggtgctggaa gatgtgcgcg ttcctgcctc ggcaatgctt ggcgaggaag gcgaccgcgc
    12241 ccggatctac gccgactgcg tttcggggtc ccggatcgcc tggtcggcga tgttttccgg
    12301 caccgcccag gccgtgctcg actacgtgat cccctacgtg aacgagcgtc aggccttcgg
    12361 cgagccgatc tcgaaccgcc agagcgtggc cttcagcgtt tccgacatcg cgatcgaaac
    12421 cggtggcatg cgcctcgcca actggcgggc cgcgagccgg gccgaccgcg gggaatcctt
    12481 cgcccgcgaa gcagccctgg cccgccggct cgccgccgca aagggcgtgg aaatcggctc
    12541 ggccggggtc cagctgctgg gcggccacgg ctacgtcaag gagcacccgg tcgaacgctg
    12601 gtatcgcgac ctccgcacag cgggcgtgat ggagggcgcc ctgctcgcct gatcaccagg
    12661 ccgagcgaaa tgaaaccatg atctatctgg aaacaccgaa gaagttccgc ccgctggtca
    12721 cccaggccaa ccacgtcgcc cgcgagatct tccgtccgat ctcgcgcaag tacgacgagg
    12781 ccgaacacga gtacccgaag gaactcgacg tactggcctc gctgatcaac ggcatgaatg
    12841 agggttcggg catgggcgcc ggtgcggccg gcgtcagcgg ggaatccggt gatgaaccgg
    12901 attcgggcag cggcaaccgc aacggttcga acctgagcac cgtgctcggc atcatcgagc
    12961 tctgctgggg tgacgtcgga ctgcttctgg cgatgcccgg ccaggggctc ggcaactcgg
    13021 cgatcgcgtc ggtcgcgacc gcggagcagc tcgagcgcta cggcggcaag tgggccgcaa
    13081 tggcgatcac cgaaccggag gcgggctcgg attccgcggc gatccgcact acagccgtcc
    13141 tcgacgggga cgaatacgtg ctgaacggcg agaagatctt cgtcacttcc ggtgaccggg
    13201 ccgagctgat cgtggtctgg gccagcctcg accgctcggt cggccgggcg gcgatcaagt
    13261 cgttcatcgt cgagcgtgac aatccgggcc tgaccctgga ccggctcgag cacaagctgg
    13321 gcatccgggc gtcggacacc gccaacttca ccctgcagga ctgccgggtg ccgaaggagg
    13381 cgctactcgg ctccccggag gtggacgtga agaagggctt cgccggagtg atgaagacct
    13441 tcgacaacac caggcccctg gtcgccggca tggccctcgg ggtaacccgc gcggcgctcc
    13501 aagagacgaa gcgccagctc gaagcggccg gtgtcgagat cgattacgac actccggcgc
    13561 tttgccagtc cgcggccgcg gcccgctacc tcgagatgga agccgattac gaggcggccc
    13621 tgatgctgac tctgcaggcg gcctggatgg ccgacaaccg gaagccgaac tcgctcgaag
    13681 cgtcgatggc caaagccaag gccggccgga ccggggtcga cgtgaccctg ggctgcgtcg
    13741 agatctgcgg cagcatcggt ctcggtgaat ccgagctgct cgagaagtgg tcccgtgatg
    13801 cgaagatcct cgacattttc gaaggcaccc agcagatcca gctgctgatc atcgcccgtc
    13861 agctgctcga taagacctcg gccgaactca agtaaccaga agcccccggc tcaggtggtc
    13921 cgcggtggcg ggatgcgtcg ggggcggatc gattcgaaag ccgaggcctg ggtgagcagc
    13981 cggttgtcgt cgtatgaacg accggcgaag gtgaggccga ccggcatccc gatgtcaccc
    14041 atcagcccca tcggcacggt cacggtcgga atgccgaggt gccggggaac gaggttgccg
    14101 ttggccaccc agacaccgtt ggcccaggcc cggtcggccg agcgcgggtt cacatcggcg
    14161 tcggccggac cgacgtcggc gaccgccgga aagaccaccg tgtccagtcc gagctcatcc
    14221 atccactcct cgaggtcgat ccggcgggtc tgctccagac cccgcaggcc tgcttcgagt
    14281 tcaggcagtt gatcccaggg ggtgaggccg gcccaggcca tctccacata ctcctccatg
    14341 ccgaaggcca ggtcaccttc ccggctgggc agggcaccgg cttcgggcgg aaagatcagc
    14401 tcgccgtccg cgtccgccag ccggttcagg gccgggtcat cattggcccg gagaaagtcg
    14461 tcgtacgccc aggccgaaag tccgcgcaac tcctcgtcga gaaaggccgg gctgaccagg
    14521 ccgcgggtga agatgttcgg acagccggcc cggtcaccct cgtagttgga gaccaccggg
    14581 aaatcgactt cgaccacctc ggccccggcc gcctcgagat cattgcgggc tgcctgccag
    14641 aggtcgatca ccgactgccg ggtatcgatg cgctcgccgg tcggtcccca gacgtcgctc
    14701 ttctcccccg tgcccgcggc cggatcggcg ttgatgtaca tgcgcgggat accgaaccga
    14761 ttgccggcca gcgcctcgga agatgcggcc agcgacgggt acgagtccgg tctgacctcg
    14821 gaggcggccg gcagctcgat ccactcctgc gcccgccaga ggtctcccct ggtgtcggga
    14881 tcatcggcga cgatcacgtc gagcacctcg agcagatcgg ccatggttcg cgcgtaggga
    14941 accaccacgt ccatggtcgg ggtaagcggc cagttgcccc ggaccgaaat cactccgcgt
    15001 gaaggcgtgt aggcgcagag gccgttattc gaggccggcc ccctgccgct cgaccacgtt
    15061 tcctcggcca gaccgaaggc ggcgaaactg gctgcggtcg cggtcccggc cccgttcgat
    15121 gatcccgagg cgaacggcgc ggtcaggtaa tcggcgttgt aggggctctc cgcgcggccg
    15181 tagacgcccc gctgcatgcc gccgttggcc atcggcggca tgttggtctt gccgaggcag
    15241 atcgcgcccc cttcccgcag ccgcttgacc gtgaaggcgt cacgcatcgc cagcaggttg
    15301 acgaaggccg ggctcccgga ggcagccgtc atgccgcgca ccaggtagct gtccttggcg
    15361 gtgtaaggga ttccgtcaag cgggcccatg gcctggccgt tggcccgcct ggcatcggac
    15421 gcggctgctt ccgccatggc ctccgcgttc cggacgacga ccgagttcag cctggtttcg
    15481 gtgtccgggc cgtcaaaggt gtcgatccgc gcataaaagg cctcaaccag ttcgaccgcc
    15541 gtgatttcac ccgcctgaag cgctgcgcgc aggtcggcga ttggtgtctc gatgatttcg
    15601 atcactcttt caccggtcgc cagtctagcc ccggtgtgaa acccgcgaaa cggtcaggaa
    15661 ccgtcgtctt cgtaaatgcc ggcgattctg tccagcaccc cgctggccgc ctcgaggtcc
    15721 cggtccggga tcccctccag cgcctcgttc cactttcggt gccagctctc gatgatcggt
    15781 tcgatcacgc tgcggccctg atcggaaagc gcgaccttga cgatccggcg gtcctcctcc
    15841 gaacggatcc gctcgacgtg cccctggtcg gaaaggcggt cgaccatctg ggaaacgctg
    15901 gccgggctca ggccgttggt ctgggcaagc tcgccgaccg ccagggggcc cgacttgtga
    15961 agttcgacca gaagctcgaa ctgggactgg ctcacctcgg tcgcgccgag ccggtgggtc
    16021 tcgcgcccct tgcgccggct cagggtgcgg taggcgttct tgaacgcccg gacgacttcc
    16081 agccggtttc cgctgagttc gactggatct tcaggcgcca agcggttcaa tttcaacctc
    16141 cgcgattcgt tcttcctcgg tcaccggccc ggccggcatc tccgcccgcc acagcaccac
    16201 gcaggggatc agggccagca gggcgatccc gagcgcccaa aggtaggcgg tcccgaaccc
    16261 ctgtgccgcc gccgttgccg tggttgcacc ctcgcttgcc tgctgcagga cgaccgccag
    16321 taccgccgtg ccgatcgctc cacccacccg ctggatcacg ttcatctgcg gcgtggcatc
    16381 ggaaagctgg tccttcctca gcgatgcgta ggcgacggtc atcgtcggca tgaaggcaag
    16441 gccgatgccg atgccccgga ccagcagcat caacgagagg aaggcgatcg aagtgtcaac
    16501 cccgatgaag acgaagggaa gcgatccgag gctgagcacg gcgactccga aggtcgcgac
    16561 cttgccgccg ccgaagcgct ccgtgaggcg gggagcgaag ggcccgacca tcagcatgcc
    16621 caggccctgc ggggccagca ggaggccggt cgcgatcacg ctttcgccgc ggatctgctg
    16681 gtagtagagc ggcatcagga tcatcgctcc aaagagcgcg gcacccagcg cgaaggtggt
    16741 tagcgaggcg gcaccgaaga cccggttcgc atagagccgg acgtcaagca gcggccgctc
    16801 ggcgcggtat gcgtgccgga tgaaggcgcc gatcaggacc agcccggcga tgaccggcca
    16861 gatcacttcc ggcgagttga aagttccggt ggtaccgact tcggagatgc cgaagaccag
    16921 ggacggaatg ccgagcacca tgaggaccag tccggcccag tcgagtttgc cgggctcgcc
    16981 gagtttcgaa tggggcagca tcctcaccgc gagcagcgcg gcgacgatgc cgaccggaac
    17041 gttgacgaag aagatccagg accagtgaaa gttctgcagg attagaccgc cgatcgtcgg
    17101 gcccagaatc ggggcgagca tggcgggcat cacggtgacg ctcatgacct tgcccatcct
    17161 tttcggtccg gcgacctcgg ccatgatcat ctgggcgatc ggcatgatca tccctccacc
    17221 gatcccctgg atcacccgga aggcgatcag cgaagtgctc gatgcagcga tgccgcaaag
    17281 ggccgagccg agcgtgaaca ggacgatcga ccagatgtag acgttctttg ccccgaattt
    17341 ccgcgccgcc cagccggtca gggggatcac cgccgcgagc gaaagcaggt agccggtcgc
    17401 cacccactgg atctcgctga tcgtcgtgtg caggtctttg gcgagggtgt cgagcgcgac
    17461 gttgacgatc gtcgtgtcga gaatcgacat gatcatgccg aggatcacca cccctgcgac
    17521 cttccagacg tacggctcga tgcgggtagt ggaatcctga ttcattagga acctaatatt
    17581 aggctcctaa tcactttcgg tcaagtgtga atccgtcaca ctcccccggg ccccggtgcc
    17641 cgctgcggga agacatcggt ttccgggcag ggcgtcttgg tgccggcatc cagctccgcc
    17701 tgcagggccg gatactcgcc ggcgatctcg gcgcgcggca gcccgtcgag agccaggacc
    17761 gtgtcccccg ccgccagcac ctgcttgcgc atctggccga aatcaatcgc cttctcgccg
    17821 aacaccgggt cgtacaggga ccagggtcca gagatcatcg ccagggtcgg aaccagttca
    17881 ggctggaagg agttgccgag ccctcccatc gagcagatcg acggggcctg gtcggggttg
    17941 ggcacgctca gccccttgag caccgccgtg cggggaaggt tccttcgctt ggtggcctcg
    18001 accgcggctt cccgcagcgc gtcactgtca ccggcgccaa agagtgccgg gtcggccacc
    18061 ccggtgaagg cgagatggcg gctcgttccc tctccggtcg gtatcacctc gcgggtaccg
    18121 agatgctcga tcgcgaaacc gaacgcgatc cgcttcttgt ccagcgggta atggcggggg
    18181 ctcatcccgt cattgcggta agagtcatgg gcggaggaga agaccagctc cagggtgcgg
    18241 ggccgacagc gcagcggcaa cttcgcgtag tagcgggcga aggcgagcat gccgatcacg
    18301 ccgttctcct ggacccagga ggtgccatcg gtgtttgcga ccatgaccat cttctcgttg
    18361 ctttggcccg gaagcctggc gatcaggttg cgcgtcttgc ggcgagcgag gttcgcgttc
    18421 accacgaccc gagccgaggt accccggcca gcgagttcct tgagtcgctc accctgggat
    18481 cgaccgacaa agaccgccgg ttgccggtag atcatgccga catgcgggtc gtagtagccg
    18541 cgtatctggc tggccggcag gtcaaagacg aagatcaccc cgtttgcgcc ggccgcgctc
    18601 gcatccagct gatcggcgtg gatgttcggg ctcaggtacg gtcgcgcata gtcggtcgtc
    18661 ccctcaaggt cgggcgtctg gtagagcccg agcagcgaac cgacgacccc gaacggaatc
    18721 gatccgatcc ggaaatccct gatcacgacc ttcccggccg cgttttcggc ggtgatctgc
    18781 tgatcggcag gaaggtagac gagcggcccg ccctcaccct tgctcgaggt tggcttcgac
    18841 caatgcacag caccggcgtc ggccacctcg aggattgacc cgtcctgctt cctgaccctg
    18901 atcgcgccgg cggccccgat gtcgagcccg gggcccttct tcatccgggt ccggggaagc
    18961 caggtgtagg gatggaagta ggtcgagtgc actctcaacc cgatcgaccg tgcctgccct
    19021 tccagccatg cgatcgcctg gttgtgggcc gggctgccga gaatgcgcgg accgaagctg
    19081 ttgaacttcg ccgtcatctg gtgaaggcgc gccttgctgg caaaagcgga ggcgttgatc
    19141 acgtccgggc attcggcggc ccgtgccgcc ggagctgcca tcagcagcat cgcggccatc
    19201 gcggcaagcg cgagccaacc tcgcctggcg cctggttccc tgatttcctc cgaattcatc
    19261 ctcatcttcc cgcttgaatc ccctcccgaa aacggccgac cagcgatggt tgcggtcccg
    19321 ctggaactac gcgagaagcc tagcgctccc ggacagccac gcatttgaca ccgaatgatg
    19381 gaatgctttc cggtcgcagg aactgtcaaa ggcttcgaac ggagaaaaag tgggtcatta
    19441 caagagcaac gtccgggaca tcgagttcaa cctcttcgag atgttcgaca ccgatggagt
    19501 gttgaagacc ggcgcattcg gcgacctcga ttcggacacg gtcaggatga tgctggagga
    19561 agcctccaag ctggccgagg gacccgtcgc cgccgctttc gaatcaagtg accggactcc
    19621 gccgagcttt gacgccgata cgcacgacgt aagcctgccg gcagacttca aggcgtcggt
    19681 caacgcctgg accgaagccg actgggagaa gatcggcctg atcgaagaag tcggcggcat
    19741 ccccgccccg gcgacggtct cctgggcgat caacgagttc atgtgcggcg ccctgccggc
    19801 cggcttcttc tacctcgccg gccccgcctt cgccagcctg ctctacaaga acggcaacga
    19861 ggaacagaag cactgggcac agatggcggt cgagaagaac tggggcgcga ccatggtcct
    19921 gaccgagccc gacgccggct ccgacgtcgg cgccggccgc accaaggcga tcgaccaggg
    19981 tgacggcacc tggcacatc
//
LOCUS       DAOQKN010000021        19115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_1273_length_19115_cov_4.860021, whole genome shotgun sequence.
ACCESSION   DAOQKN010000021 DAOQKN010000000
VERSION     DAOQKN010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 19115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19115
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1273_length_19115_cov_4.860021"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..640)
                     /locus_tag="PLJ64_02640"
     CDS             complement(38..640)
                     /locus_tag="PLJ64_02640"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HNC92422.1"
                     /translation="MYAAQSLPGPLEVSAAAPMPEGSRTALLVIDMQNDFVEAGAPRQ
                     VSGATEIVPQVASAAERMRMQGDLIVWVCRAYSDDGSNVEAARKPQFLKNPYAVAGTR
                     GAALVDGLAPWPDDLSVVTPSYSAFFRTDLDLKLREAGVSKLVGCGVDLSRCVRSTLV
                     EGISLGYECSVLAPAVASRDDQALNSNLADLVDLDITVEE"
     gene            complement(776..2452)
                     /locus_tag="PLJ64_02645"
     CDS             complement(776..2452)
                     /locus_tag="PLJ64_02645"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNC92423.1"
                     /translation="MTQPAPQPEPTPGPGRNLPPWPQERGLRSWQKLALAELAAHPGG
                     SFLASATPAAGKTTFGLRVAWEMLASGRVSRVVVIAPTTHICRQWAADAARYGIDLEP
                     NRPNSDGPETSDFHGVTVTYQTVAAGPGVHADAARRPTLVIADEPHHMGDQASWGISA
                     RRAFDHATFRLLLSGTPFRSDNEAIPWVSYDEDGLSSADFTYGYPEALVDRVCRPITF
                     LPYDGEMEWSSDGRVWNADFDLVLPAAEAARRLRTALAADGEWMGEVLRDADARLSEV
                     RAAGHPDAGGLVVASDQDHARAIARRLEFITGEQPEIVMSDEPGASARIAAFAESEDR
                     WLVSVLMVSEGVDIPRLRVGVYATAARTELFFRQVVGRFIRTTPSPRRQMSYLLLPAD
                     SRLKQLAFEIEKERRHAIELGPELADELEELDHELPERSEPGEAFQALSSSGAELDDA
                     ILAQTTMQLFATDTFDSGPKPEKTLAPAPAAKKPLKTLAPRSDSAFAVRERLREERKE
                     LVADVSRKTGESYQQINSRINRELGVASVSKATRQELEQGNALLERDLKI"
     gene            2586..3911
                     /locus_tag="PLJ64_02650"
     CDS             2586..3911
                     /locus_tag="PLJ64_02650"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNC92424.1"
                     /translation="MSTGANIISPSDPGFAEAKFAYDLHDQKPAAICLPTTVAEVQQA
                     VRWARDEGLRIANQATGHVAVALPDLEDALLLKPQIDTEPVVDPAAGYCRVGAGTRWQ
                     PVVEAAAAHGMAAMHGSSPTVGVVGYHLGGGLSFYSREHGLACNKVRAIEVVTADGEL
                     ARIDRENEPDLFWALRGGGGLFATVTAIEFDLLPVGEVYGGASFWPVETAEAALSTWL
                     DWTRTAPESVTSTFRIMNLPPFEVVPEPIRGKQLVVINGVALDKGDGEALTAQLDGLG
                     DPVMAQWGMMPGPAVASLHGDPEDPVPAIGNSTMLDGVGDELVEAFVGAASGESGCTL
                     IAAELRQLGGALRRPDPDGGVASMIDGEFIAFGVGLAPEPGMKAKTEEDLTRLMEAVK
                     PWEGGRQYYNFAVGGAACQDCFPEDDIEALKAIRHEYDPESLYLAPLPL"
     gene            complement(3944..4369)
                     /locus_tag="PLJ64_02655"
     CDS             complement(3944..4369)
                     /locus_tag="PLJ64_02655"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNC92425.1"
                     /translation="MAARGSAEWQGTIREGSGTFTAGDSLGGEYSFKSRFEDGPGANP
                     EQLIAGAHASCFSMALSLVLEEAGTPVESVKTDAKVTIRMLEEGPTITRIELSTVGRV
                     PGADQAAFEEAAGKAKAGCPVSKALAGVPEITLEATLEP"
     gene            complement(4431..6539)
                     /locus_tag="PLJ64_02660"
     CDS             complement(4431..6539)
                     /locus_tag="PLJ64_02660"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNC92426.1"
                     /translation="MKSLLVLPAGPRARWVTVAVWFLAMFVVFGTNIPGKFADAENNE
                     SVSYLPGDAEATRALEAQKKLVGSEEAPMVIIYRREGGLTPADKARIKADVHELNEKV
                     EGVSKRFVITQRSKDGSAVIVTSSITSTGEGEDIREPVEAARDLVSGSFDGLQVDITG
                     PAGYASDSIKVFESINGTLFAAAFGLVFILLILIYRSPFLLWLPLIAVGFAEMGARAV
                     GYGLTELGVTVNGQSSSILSVLVLGAGTDYALLLISRYREELRHRESRTESMRVALAS
                     AGPAIVYSGLTVVAALLCLSLAELNSTASMGPIGAMGILMAMLSMLTLLPAMLVIAPR
                     FVFWPKVPRVGTGGADAEHGPWRRLGDRIAKRPRPVWITAVLVLLVMCAGLFSYNDNL
                     TSANGYVDEVEAVRGEESIAKAFPGGSNVPTEIVVGDPKKLDEVEAAVRKVDGVAGSQ
                     VAFRTPQGVVLNVILEDEPYSKSAFALIPAIRDAAHEAGGDTTLVGGGTAVEHDIREA
                     NRHDVKLIVPIVLLVVFLILVGLLRALAAPVMLIGTVVLSFFAALGIGFLVSDYIFGF
                     PGVDPSLPLYVFVFLVALGIDYNIFLMARVREETATHGTREGMLRGLAVTGGGITSAG
                     IVLAGTFSVLAVLPLVFLAEMGFTVAFGVLLDTFLVRSILVPALTLDIGSKIWWPSAL
                     AEVGDDPASGKPVEPDPAAG"
     gene            6601..7134
                     /locus_tag="PLJ64_02665"
     CDS             6601..7134
                     /locus_tag="PLJ64_02665"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNC92427.1"
                     /translation="MDRKLGVLIGLIVLIGALVAVILIGRGSGDSGDSAASGEIPSEV
                     TAGVSEDLSERPKLAGTSETPPTALVKKDIVVGDGAEAKEGDTVKVQYVGALYDNGKE
                     FDASWDRNKEAFSFQLGGGQVIKGWDEGVAGMKVGGRRVLVIPPDLGYGPQGSPPTIP
                     GNSTLVFVVDLEDVQGQ"
     gene            7180..7680
                     /locus_tag="PLJ64_02670"
     CDS             7180..7680
                     /locus_tag="PLJ64_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HNC92428.1"
                     /translation="MDVIDELVENNREFSSRVPDQDLDVKPSRQLTIVTCMDSRIDVY
                     QALGLKDGEAHILRNAGGVITDDVIRSLAISQRRLETRAIMLIHHTNCGMQTITDDGF
                     RSELQEATGTAPAFAIESFTDVEADVAQSIRRVRRSEFIPHRDNVRGFVYNVEDQTLT
                     EVTVED"
     gene            7690..8025
                     /locus_tag="PLJ64_02675"
     CDS             7690..8025
                     /locus_tag="PLJ64_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HNC92429.1"
                     /translation="MGIVVYEKPTCTTCRNLFRLLEAEGIDFESVEYHVTGLTEDEIR
                     GLLGKAGITAAEALRMREEGARELAGEDEDTIIAAMAERPELLQRPFVVNGEKAVLAR
                     PVERALEVI"
     gene            complement(8035..8286)
                     /locus_tag="PLJ64_02680"
     CDS             complement(8035..8286)
                     /locus_tag="PLJ64_02680"
                     /inference="COORDINATES: protein motif:HMM:NF025385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4235 domain-containing protein"
                     /protein_id="HNC92430.1"
                     /translation="MGLIAGLIAGLVARKIFEQGWSSLSDEDPPSPDEESLSLGKLVL
                     ALLLEGAVFSLVRGLTDHGARVAFRRYTGAWPGETGKDN"
     gene            8395..9036
                     /locus_tag="PLJ64_02685"
     CDS             8395..9036
                     /locus_tag="PLJ64_02685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03914.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HNC92431.1"
                     /translation="MDPETAGITGARRFVPEGRLSLGRLASAATNCRGCPLWRGASQV
                     VFGEGSRTAELMLVGEQPGDREDRDGRPFVGPAGLELNRILDETGIERNRLYVTNAVK
                     HFKFHREGKRRIHDKPTRNEVKACRPWLDRELELVQPRAVVALGATAGRELFGPGFRV
                     TRHHGQFLENGPAEISVGTIHPSAIIRARDPDREAYREMLTGDLCAVIERLSS"
     gene            9096..9572
                     /locus_tag="PLJ64_02690"
     CDS             9096..9572
                     /locus_tag="PLJ64_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92432.1"
                     /translation="MSDRELGIYLNDHLTGATGGVALARRAAANATDQERKAMWKSIA
                     HEVEEDRVTLTHVRDLIAVKPNRAKYALAWTAEKLGRLKLNGRLTGNSELGQMLELEA
                     LVIGVTGKLSLWKALARLEDPRLEGIDFDRLVERAESQRRRLEQFRINLVPAAIGR"
     gene            9629..9889
                     /locus_tag="PLJ64_02695"
     CDS             9629..9889
                     /locus_tag="PLJ64_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92433.1"
                     /translation="MTGPWRDLEQRLEFENEAERPLRESGEGESEGFEEAEAELIHQS
                     EDPDLGRDPELDAIVEAEDAGSVYSEADEILSSEEPDSDHDG"
     gene            complement(9912..10769)
                     /locus_tag="PLJ64_02700"
     CDS             complement(9912..10769)
                     /locus_tag="PLJ64_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HNC92434.1"
                     /translation="MPELPEVEITARLLDQAVAGAKVESAMAPGLNVMKSFKPPLDGL
                     AGDRIDGIRRRGKMLVIDFSSGLSLLIHLMSAGTLQLHGKRAGPKDRTSRVLIRLEPD
                     QGGERELRLREFGTRQRAWAKLMPADEIETDEDVAKLGPEAWPDPVPVGVVTTTDRPL
                     YSLLRDQRTIAGIGRTWVDEILWSARLSPFKRAADLEPEEAERLLASIHDRFQAAIDF
                     YESGLTLPLPDKLPKPLEVHGREGEACSRCGELIHAVHYSDYTLCYCPREQTGGEPLK
                     DRRLSRLLK"
     gene            complement(10773..11492)
                     /locus_tag="PLJ64_02705"
     CDS             complement(10773..11492)
                     /locus_tag="PLJ64_02705"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC92435.1"
                     /translation="MTATRQQAEAAADQVPGSKLSARLYEPFLWLGEKTGFRRWRQKV
                     VSGVSGSVLELGAGTGLNLVHYPAGLDRLVLVEPDFHKAGILATKPAPAGVEPEIVRA
                     PGEMLPFEDDTFDTVVETLVLCTVADPEATAAEIRRVLKPDGRFLFMEHVRSEGPRLG
                     PLQDRMEKTWMKIADGCHCNRRTVPMLEANGFEVEVTAEQDKFPMPPVARPIVSGIAR
                     PIAGSPPAPERGSAPDGDAAT"
     gene            complement(11489..11773)
                     /locus_tag="PLJ64_02710"
     CDS             complement(11489..11773)
                     /locus_tag="PLJ64_02710"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC92436.1"
                     /translation="MPETSEDNVEVTDQPDASRYEIREAGELAGFAEYRLHGDVITFT
                     HTEVDPERGGRGLGSRLIEFAVTDARSRELSIIPRCPFVRDWIAEHPEED"
     gene            11840..12391
                     /locus_tag="PLJ64_02715"
     CDS             11840..12391
                     /locus_tag="PLJ64_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92437.1"
                     /translation="MTVFEPTLSSEKLTEATGKNWDEWLELLDQAGAIKRTHTEIARW
                     LTDEQGVPGWWAQSITVGYERARGLREPGQRADGWSVTASKTIEVPVEELFEAFERDT
                     IREKWLPDAELHLRTATAPVSARYDWGDGTTRLIVGFESLGPNRSKVALEHEKLPDGE
                     VAERMKVWWRERVVDLKELLEAG"
     gene            complement(12422..12925)
                     /locus_tag="PLJ64_02720"
     CDS             complement(12422..12925)
                     /locus_tag="PLJ64_02720"
                     /inference="COORDINATES: protein motif:HMM:NF021538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HNC92438.1"
                     /translation="MENLPLAVTGYQLSVWIHVTAVVVGLGMTFAEAVFFPVAMKLDP
                     RHLPYVHRLQLTINRYVASPALLLIIVTGIYQVTDAGWDFGSFWISATFVIVFLIGAI
                     NGAYFIPADKRLEAMITAEVEAAGPGEFTPSEEYMRQARIEGIIGTVTGILIVIATYL
                     MVVKPGA"
     gene            12996..13439
                     /locus_tag="PLJ64_02725"
     CDS             12996..13439
                     /locus_tag="PLJ64_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92439.1"
                     /translation="MRKPFALGVITLAVTLSLGLAACGSSSDDSLSNDELITQADQIC
                     TDYNDKLVTLTDNAGLDNNSSKQEVVAFISDEIVPLYENQVDELRALEPNEDDADAYN
                     DIVTTLDTELQAVKDDPAAAMKMDDPFPEATAKAKDFGLTVCGAS"
     gene            13518..14252
                     /locus_tag="PLJ64_02730"
     CDS             13518..14252
                     /locus_tag="PLJ64_02730"
                     /inference="COORDINATES: protein motif:HMM:NF024098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HNC92440.1"
                     /translation="MYLLFLDESGIPGEDGIFALGGVAIRADQWAEVKDRFETCLEEA
                     GWPADKEFKWSEISRSTAAADAAYPVYDCLATLPIRSLVTVLYTEGDDPVNREKFFAN
                     PDLIYRTAFTFVAERYQRFLAHHESHGVIVLDSRQYETDDRLRRYFDRIHNEGTDFAE
                     LDRIVDGLLLGPSHFSLGLQLADLVVGPTRTAQFSGPGTARHCFKTLKPTFMTHPASG
                     ALEGVGLKVFPHQETTREDDRLFRPA"
     gene            14352..16319
                     /locus_tag="PLJ64_02735"
     CDS             14352..16319
                     /locus_tag="PLJ64_02735"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC92441.1"
                     /translation="MLDENRLAVLNAVCDTIVPSLERDDDPTGFWARSAADTKANEVV
                     AQTIGEMPAADQEGMGLLLDSLALQNFPSLSQASREQILVNTSLASREAAVGVAALTQ
                     MTLFFHYGLPPNPAWEQFGFPGPASPPPQVEKTIHPLTPGDGDVLEADAVIVGSGAGG
                     GVIAARLAEAGMKVIVLEMGGYFNESDFDQTELNGFARMYWRGGPTYSADFNISIQAG
                     SCLGGGTLINWTNSLKPKPWVRQEWADEYGLEDVNTPDFDRHIDSIWERSKVNHDCSE
                     LNQTQKTWIEAAEKLGWSWHKTDRNWDPEKHDPLVAGYMGWGDQSGAKQSTLKTYLQD
                     AADHGAEIIVGCQAQKVLVENGRAAGVEATIEGVGRITVRSPRVVVAGGALESPALLL
                     RSGIGGPAAGKYLRLHPCTLIFATYPEDQQAWWGPPHAAVVDEFDQGLEGDGYGFLIE
                     GAQYTTGLGGSAIPFTTAAEHREIMANYANGSITINLTRERGGGQVLIDGDGQSMPIY
                     SLDDPRDIANMHFGLEQQVRLHHAAGANQVFALATGMPGWRVGDDFEAFVDRVHRIPL
                     RAGGAKLFAAHQMGSCRMGKDPAESVADPRGELHDTPGVWIGDGSAFPTPSGTNPMIT
                     IMALASRTAEKILEDSGTSGRSASETVEAGA"
     gene            16316..17809
                     /locus_tag="PLJ64_02740"
     CDS             16316..17809
                     /locus_tag="PLJ64_02740"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNC92442.1"
                     /translation="MNPTEAPETEANQAAGGGEAAAPGWAAKTGSHFIGGEWRDPIST
                     DTIEVINSVTEEVMGTVPAGSAEDVDAAVAAARAALPGWKALSVQDRAGFCTLIGHGL
                     QDRAEEIAGLVSQELGTPVTEGMLIQASLPAIDFTSMEFLVEELPWEQTIGNSKVIRE
                     PLGVVGAITPWNYPIHQIAAKVAPAITAGCTVVLKPSEMTPLSAYLLMEVIESVGLPA
                     GVVNMVTGTGPVVGEAIASHPGIDMVSFTGSTRAGIRVSELAAASVKPVTVELGGKSA
                     NVILDDADFEAAVVNGIQKCFINGGQTCSALTRLLVPREALPAVEAVAAAAVEQTAVG
                     DPTDPTTQIGPMSSAVQRDRVQGYIEKGIAEGAKLVAGGPETPEGLETGFFVKPTVFS
                     EVTREMTIAREEIFGPVLSILPYDSEEEAIEIANDSSYGLAGGVWSSDPERAIGVARQ
                     IETGQVEINGAAFNPLAPFGGRKLSGHGRELGPYGIEDFLTLKAIQL"
     gene            18033..>19115
                     /locus_tag="PLJ64_02745"
     CDS             18033..>19115
                     /locus_tag="PLJ64_02745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92443.1"
                     /translation="MKSILALGLTGLALACAQSADAARFEVTDLGVLPGGTDSTAQGI
                     SDTGHVVGQSAGYPYVWRPGSGMQKVEVLAPDVDYAGPDINDADLLAVTGPHPDEDAF
                     GFLWHGFLWASGNAIDLGAVGEAPCLEGCDVDPNDQSFANGINNFGTVVGASTVENAS
                     GPTSMAPTIWPGGGNPVRLPGSGGGRAREIADNGRIVGTRGTHAMLWEDSGSQPTDLG
                     LLPGSTGDQSDAYDLNEAGTVVGASETANGVTRAFMWTPGAGMQPIATPNTLSGFSSA
                     NAIGPDGTIVGSTYNGTKTVGFIREAGQPITDLNTLIPEGTGWDLGSARDINSKGQII
                     GNGILNGESRAYLLTPTQPLKATLQPV"
BASE COUNT         3301 a         6537 c         6335 g         2942 t
ORIGIN      
        1 cagggtcagg tggccctggc gccgggccgc tgcctgacta ctcctcgacc gtgatgtcga
       61 ggtcgaccag gtcggccagg ttcgagttga gcgcctggtc atcccggctg gcgaccgccg
      121 gggcgagcac cgagcactcg tagccgagag agatcccctc gaccagggtc gaccgcacgc
      181 agcgggaaag gtcgaccccg cagccgacca gcttggaaac cccggcctcg cgcagcttca
      241 ggtcgaggtc ggtgcggaag aaggccgagt aggaaggagt gacgacgctg agatcatcgg
      301 gccagggggc gagcccgtcg accagtgccg cgccgcgggt tcccgcaacc gcgtacgggt
      361 tcttcaggaa ctggggcttg cgggccgcct ccacgttcga accgtcatcc gagtaggcgc
      421 ggcagaccca gacgatcagg tcgccctgca tcctcatccg ctccgccgcc gaggcgacct
      481 gcggcacgat ctcggtcgcg cccgagacct ggcggggtgc tccggcctcg acgaagtcgt
      541 tctgcatgtc gatcaccagc agggccgtcc tggacccttc cggcatcggt gccgcagccg
      601 aaacctcaag tggccccgga aggctctggg cggcatacat gacttgcgaa cttcctctcc
      661 ctaatcgaac atcattgaac agcgcgatcg gagattaccc aaaaaatcct gccccgaaac
      721 gcagaacgcc aagtttcttc tagtacgcga atggctttgg aaagccgaaa cctggtcaga
      781 tcttcagatc ccgctccagc agcgcatttc cctgctccag ctcctgacgg gttgccttgc
      841 tgaccgaggc aactccgagc tcgcggttta tgcgcgaatt gatctgctgg taggactccc
      901 cggtcttgcg ggacacgtcg gcgaccagtt ccttgcgttc ctcacgcagc cgctcccgca
      961 ccgcaaaggc cgaatcggac ctcggggcca gggtcttcaa cggcttcttc gccgcaggag
     1021 ccggggccag cgtcttctcg ggtttgggac cggagtcgaa cgtatcggtg gcaaacagct
     1081 gcatcgtcgt ctgggcgaga atcgcgtcat cgagctcggc gccgcttgag ctgagcgcct
     1141 ggaaggcttc ccccggctcg gagcgttcgg gcagttcgtg gtcgagttcc tcaagttcgt
     1201 cggccagctc cggtccgagc tcgatcgcgt ggcggcgctc cttctcgatc tcgaaggcga
     1261 gctgcttgag ccggctgtcg gcgggcagga gcagatagct catctgcctc cggggcgagg
     1321 gggtggtgcg gatgaagcgg ccgacgacct ggcggaagaa gagctcggtc cgggcggccg
     1381 tcgcgtagac accgacccgg agccgcggga tgtcgacgcc ctcggagacc atcagcaccg
     1441 aaaccagcca gcggtcctcg gactccgcga aggccgcgat ccgggcgctc gccccgggct
     1501 cgtcgctcat cacgatctcc ggttgctcac cggtgatgaa ttccagccgc cgggcgatcg
     1561 cccgagcgtg gtcctgatcg gaggcgacca cgagaccgcc ggcgtcgggg tggccggccg
     1621 cccgcacctc actcagccgc gcgtcggcgt cgcgcagcac ctcccccatc cattcgccgt
     1681 cggcggccag cgcggtgcga agccgccggg ccgcttcggc ggccggcagg accaggtcga
     1741 agtcggcatt ccagaccctg ccgtcactcg accattccat ctcgccgtca tagggcagga
     1801 aggtgatcgg ccggcagacc cggtcgacca gcgcctccgg gtagccgtag gtgaagtcgg
     1861 cgctgctcag tccgtcctcg tcgtagctga cccaggggat cgcctcgttg tcggagcgaa
     1921 agggggtacc ggagagcagc aggcgaaagg tcgcatggtc gaaggcgcgg cgggcgctga
     1981 tcccccagct tgcctgatca cccatgtgat gcggttcgtc ggcgatcacc agggttggcc
     2041 ggcgggctgc gtcggcgtga actcccggcc cggccgccac cgtctggtag gtcacggtca
     2101 cgccgtgaaa gtcggaggtc tcgggcccgt cggagttggg ccggttcggc tcgaggtcga
     2161 ttccgtagcg cgccgcgtcg gccgcccact gccggcagat gtgagtggtc ggggcgatca
     2221 ccaccacccg gctgacccga ccgctcgcga gcatctccca ggctacccgc aggccgaagg
     2281 tcgtcttgcc ggcggccggg gtggccgagg cgaggaagct cccgccggga tgggccgcca
     2341 gctccgccag ggcgagcttc tgccagctcc gaaggccccg ctcctgcggc cacgggggga
     2401 ggttccggcc cggtccaggg gttggttcgg gctgtggcgc gggctgggtc aaggcagcgc
     2461 aacctaccct gcccgacggt cggaacgccg tccgatccac ccgtgatatg agtgccgatt
     2521 tcaccctggc ctcaacctga atcaggcact tgcgaagagg ctgtccggtg ggctagtttg
     2581 ggcgaatgag caccggcgcg aacatcatca gtccatcgga ccccggcttc gcagaagcga
     2641 aattcgccta cgaccttcat gaccagaagc ctgccgcgat ctgcctgccg acgacggttg
     2701 cggaggtaca gcaggccgtt cgctgggccc gggacgaggg ccttaggatc gccaaccagg
     2761 cgaccggtca cgtcgccgtg gccctgcccg acctcgagga cgccctgctg ctcaaaccgc
     2821 agatcgacac cgaaccggtc gtcgatccgg ccgccggtta ctgccgggtc ggggccggaa
     2881 cccgttggca gccggtagtg gaagccgccg ccgctcacgg catggccgcc atgcatggct
     2941 cctcgcctac cgtaggcgtg gtcggctacc acctcggcgg cggcctcagt ttctacagcc
     3001 gcgagcacgg actcgcctgc aacaaggtgc gggcgatcga ggttgtcacc gccgacgggg
     3061 aactggcccg gatcgaccgc gaaaacgagc ccgacctgtt ctgggcgctc cgcggcggcg
     3121 gcgggctctt cgccacggtg accgcgatcg aattcgacct gcttccggtc ggcgaggtct
     3181 acggcggggc gagcttctgg ccggtcgaaa cggccgaggc ggcactctcg acctggctcg
     3241 actggacccg gaccgctccc gaatcggtga cctccacttt ccgcatcatg aatctgcctc
     3301 ccttcgaagt ggtgcccgaa ccgattcggg gaaagcaact ggtggtgatc aacggcgtcg
     3361 cgctcgacaa gggtgacggc gaggccctga ccgcccagct ggacggcctt ggtgatccgg
     3421 tcatggccca gtggggcatg atgcccggcc ccgccgtcgc ctcgcttcac ggcgaccccg
     3481 aggacccggt gccggcgatc ggcaattcga ccatgctcga cggggtcggc gacgaactgg
     3541 tcgaggcctt cgtcggggcc gccagcgggg aatcgggctg caccctgatc gcggcggaac
     3601 tgcgccagct cggcggcgcc ctgcgccggc ccgaccctga cggcggcgtc gccagcatga
     3661 tcgacggcga gttcatcgct ttcggggtcg gcctcgcccc cgagccgggc atgaaggcga
     3721 agaccgagga ggacctgacc cgcctgatgg aggcggtcaa gccctgggag ggcggccgcc
     3781 agtactacaa cttcgcggtc ggcggggcgg cctgccagga ctgtttcccc gaggatgaca
     3841 tcgaggccct caaggcgatc cgtcacgaat acgaccccga gagcctctac ctggcgccgc
     3901 tacccctctg agatggcccg gccggaaggg gccgccctcg gcctcagggc tcgagggtcg
     3961 cttcgagggt gatctcgggc accccggcca gggccttgga gaccgggcag ccggccttgg
     4021 ccttcccggc cgcctcctcg aaggcagcct gatcggcgcc gggcacgcgg cccaccgtgg
     4081 agagctcgat cctggtgatc gtcgggccct cttcgagcat cctgatcgtc accttggcgt
     4141 cggtcttgac cgattcgacc ggtgtgcccg cctcctcgag gaccaaggaa agtgccatcg
     4201 aaaagcatga cgcgtgggcg ccggcgatca gctgctccgg gttggccccg ggaccgtcct
     4261 cgaaccttga tttgaacgag tactcgcctc ccagcgagtc gccggcggtg aaggtgccgc
     4321 tgccctcccg gatcgtgccc tgccactctg cgcttcctcg tgctgccatt ggtcctcctc
     4381 gactggtcgg ttgccccggg tgtccgactg gttcccggta gaggttcaga ctaaccggcg
     4441 gccgggtccg gctcgaccgg ttttccggaa gcggggtcat cccccacctc ggccagcgcg
     4501 gaaggccacc agatcttcga accgatgtcc agggtcaggg ccgggaccag aatcgaacgg
     4561 accaggaacg tgtcgagcag cacgccgaag gcgacggtga agcccatctc ggccaggaag
     4621 acgagcggca gcacggccag caccgagaag gttccggcca gcacgatgcc ggccgaggta
     4681 atccccccgc cggtcaccgc gaggccccgc agcatgcctt ccctggtgcc gtgggtcgcg
     4741 gtttcctcgc gcacccgggc catcaggaag atgttgtaat cgatccccag cgcaaccagg
     4801 aacacgaaga cgtagagcgg cagcgagggg tcgaccccgg ggaatccgaa gatgtagtcg
     4861 ctgaccagga aaccgattcc cagcgccgcg aagaagctga gcacgacggt tccgatcagc
     4921 atcaccggcg ccgccagcgc cctgagcagg ccgaccagaa tcaggaagac gaccagcagc
     4981 acgatcggaa cgatcagctt gacgtcatgc cggttggcct cgcggatgtc gtgctcgacc
     5041 gcggtcccgc cgccgaccag ggtggtgtct cccccggcct catgggcggc gtcgcggatc
     5101 gcgggtatca gcgcaaaggc ggatttcgag tagggctcgt cttcgaggat cacgttcagg
     5161 accacccctt gaggggtcct gaatgcgacc tgggagccgg cgaccccgtc aaccttgcgg
     5221 accgccgctt cgacttcgtc gagcttcttc ggatcaccga ccacgatctc ggtcggaacg
     5281 ttggagccgc cggggaaggc tttggcgatc gattcctcac cccgcaccgc ttcgacctcg
     5341 tccacatagc cgttggccga ggtcaggttg tcgttgtagc tgaacagccc cgcgcacatc
     5401 accagcagaa ccagaaccgc ggtgatccag accggcctgg gccgtttcgc gatccggtca
     5461 ccgagccgcc gccacgggcc gtgttccgca tcggcgccgc cggtcccgac ccgcggcacc
     5521 ttcggccaga agacgaagcg gggcgcgatc accagcatcg ccggcaggag cgtcagcatc
     5581 gagagcatcg ccatcagaat ccccatcgcg ccgatcgggc ccatcgacgc ggtcgagttg
     5641 agctcggcca gcgaaaggca gagcagggcg gcaaccacgg tcaggccgga atagacgatc
     5701 gccgggccgg cactggccag ggcgacccgc atcgactcgg tccggctctc ccggtgtctc
     5761 agttcctcgc gatatcgact gatcaaaagg agcgcgtaat cggttccggc gcccaggaca
     5821 aggaccgaaa ggatcgagga agactgcccg ttgacggtca ccccgagttc ggtcagcccg
     5881 taaccgaccg cgcgcgctcc catctccgcg aagccgaccg cgatcagcgg cagccagagc
     5941 aggaacggtg accggtagat caggatcagc aggatgaaga ccaggccgaa ggcggccgcg
     6001 aaaagggtcc cgttgattga ttcgaagacc ttgatcgaat cggaggcgta tccggccggc
     6061 ccggtgatgt cgacctgcag cccgtcgaag ctgccgctga ccaggtcgcg cgcggcctcg
     6121 accggttcgc ggatgtcttc tccctctccg gtggaagtga tcgaggaggt gacgatgacc
     6181 gccgagccgt ccttcgatcg ctgggtgatg acgaacctct tgctgactcc ctcgaccttc
     6241 tcgttgagtt catgaacatc ggccttgatc cgggccttgt cggccggggt gaggccccct
     6301 tcgcgccggt agatgatcac catcggcgct tcctccgagc cgaccagctt cttttgcgct
     6361 tccagggctc gggtggcttc cgcgtcaccg gggagatatg acaccgactc gttgttttcg
     6421 gcgtcggcga acttgccggg aatgttcgtc ccgaagacga cgaacatcgc gaggaaccag
     6481 acagcgactg ttacccaccg ggcccggggc ccggccggca ggacgaggag agacttcatg
     6541 ccagagaccc tagcgaccgg cccgaggcaa cccggccggg ttcaccgtaa tatgcgcggc
     6601 atggatcgga agcttggggt tctcattggc ctgatcgttc tcatcggcgc gctcgtcgcc
     6661 gtcatcctga tcggccgcgg cagcggcgac tcgggtgatt cggccgcttc gggcgagatc
     6721 ccgagtgaag tcaccgccgg ggtttcggag gatctgagcg aacggccgaa gctggctggc
     6781 accagcgaga cgccccccac cgcgctggtc aagaaggaca tcgtggtcgg tgacggcgcc
     6841 gaggcgaagg aaggcgacac ggtcaaggtc cagtatgtcg gtgcgctcta tgacaacggc
     6901 aaggaattcg acgcctcctg ggaccgcaac aaggaagcct tcagcttcca gctcggcggc
     6961 ggccaggtga tcaagggctg ggatgaaggt gtcgcgggca tgaaggtcgg cggccggcgc
     7021 gtgctggtca ttccgcccga tctcggctac ggcccccagg gcagccctcc gaccattccg
     7081 ggcaactcga ccctggtctt cgtggttgac ctcgaagacg tccagggcca gtaacacccg
     7141 gcctcaatcg gggcttcccg gctcaaaagg tttgaaccaa tggacgtaat cgacgaactg
     7201 gtcgagaaca accgggagtt ctcttcaagg gttcccgacc aggaccttga cgtaaagccg
     7261 agccggcagt tgacgatcgt cacctgcatg gattcgcgaa tcgacgtcta ccaggcgctc
     7321 ggcctcaagg acggcgaggc ccacatcctc aggaacgccg gcggggtgat caccgatgac
     7381 gtgatccggt ccctcgcgat ctcccagcgc aggctggaga ccagggcgat catgctgatc
     7441 caccacacca actgcggcat gcagacgatc accgacgacg gcttcaggtc cgagcttcag
     7501 gaagcgaccg gcaccgcacc cgccttcgca atcgaatcgt tcaccgacgt cgaggccgat
     7561 gtcgcgcagt cgatccgccg ggtgcgccgc tcggagttca tcccccaccg cgacaacgtg
     7621 cgtggcttcg tctacaacgt ggaagatcag accctgaccg aagtcacggt cgaggactag
     7681 ccggacaccg tgggcatcgt cgtttacgag aagccgacct gcaccacctg ccgcaacctc
     7741 ttcaggctgc tggaagccga gggcattgat ttcgagtcgg ttgagtacca cgtaaccggc
     7801 ctgaccgagg acgagattcg cggcctgctg ggcaaggccg gcatcaccgc ggccgaggcg
     7861 ctccgcatgc gcgaggaagg tgcccgcgag ctggccggcg aagatgaaga caccatcatc
     7921 gccgcgatgg ccgaacggcc ggagctcctc cagcgcccct tcgtcgtcaa cggcgaaaag
     7981 gctgtcctcg cccgccccgt tgaaagggcg ctcgaggtca tctgaccagg acgatcagtt
     8041 gtctttgccg gtttctcccg gccaggctcc ggtgtagcgc cggaacgcga cccgggcgcc
     8101 gtgatcggtc agtccccgga ccaggctgaa gaccgcgccc tccagcagga gcgccaggac
     8161 cagcttcccg aggctgagtg attcctcgtc cggcgagggc gggtcttcgt cgctgagcga
     8221 actccatccc tgctcgaaga tcttgcgtgc aacgaggccg gcgatcaaac cggcgatcaa
     8281 acccatcggg cggaaaagta cggtcatgga ctgaccggtg cccgttcggc cagcatcgaa
     8341 acaccgccgt cgcggcgcgg ttggccggac cgggaatcgg gtactgccgc cgtcatggat
     8401 cccgaaaccg ccggcatcac cggcgcccgg cgtttcgtgc ccgagggccg gctgtcactg
     8461 ggccggttgg cgagcgctgc cacaaattgc cggggttgcc cgctctggcg cggtgcgagc
     8521 caggtggtgt tcggagaagg cagccggacc gcggagttga tgctggtcgg cgagcagccc
     8581 ggtgaccgcg aggaccggga cggccggccg ttcgtcggac cggccggcct cgaactcaac
     8641 cggatcctcg acgagaccgg gatcgagcgg aaccggctct acgtgaccaa cgcggtcaag
     8701 cacttcaagt tccaccggga aggcaagcgc cggatccacg acaagcccac ccggaacgag
     8761 gtcaaggcct gccgaccgtg gctcgaccgg gaactggagc tggtgcagcc ccgcgccgtg
     8821 gttgccctcg gcgccaccgc cggccgggaa ctgttcggcc ccggtttcag ggtcacccgc
     8881 caccatggcc agtttctcga gaacggcccg gcggagatct cggtcggcac gattcatccc
     8941 tcggcgatca tcagggcccg ggaccccgat cgcgaggcct atcgcgagat gctgaccggc
     9001 gatctgtgcg ccgtgatcga acgcctctct tcctgaccgg ggagatggcg gaccggtccc
     9061 ggtttgattt cggggtgtcg cggtaccaga gaaagatgtc ggatcgcgaa ctgggcatct
     9121 acctgaacga ccacctgacc ggcgccaccg ggggtgtggc tctggcgcgg cgagcggccg
     9181 ccaacgccac cgaccaggag cgcaaggcga tgtggaagtc gatcgcgcac gaggtggagg
     9241 aagaccgcgt gaccctgacc catgtccgtg acctgatcgc ggtgaaaccg aaccgcgcca
     9301 agtacgccct cgcctggacg gccgaaaaac tcggccggct gaagttgaat ggccgcctga
     9361 ccggaaactc cgaactcggc cagatgctgg agcttgaggc gctggtgatc ggcgtgaccg
     9421 ggaagctctc gctctggaag gccctcgcca ggctggagga tccccgtctg gaaggaatcg
     9481 acttcgatcg cctggtcgag cgggcagaaa gccagcggcg ccggctcgag caattccgga
     9541 tcaacctggt tccggccgcg atcggcaggt agcgatcgac cggcgaaggg acggtccagc
     9601 cgcatcgccc ggccagtatc ttgtcgccat gaccggtccc tggcgtgatc ttgagcaaag
     9661 gctggaattc gagaacgagg ccgagcggcc tttgcgcgaa tccggcgaag gtgagtccga
     9721 aggcttcgag gaagccgaag cggagctgat ccaccagtcc gaggatccgg acctcggtcg
     9781 cgaccccgaa ctcgacgcga ttgtcgaagc ggaggacgcg ggctcggtct attccgaagc
     9841 cgacgagatc ctcagttccg aggaaccgga ctccgaccac gacggctaga agccggctcg
     9901 acccgccccg cttatttgag gagccgggag aggcgacggt ccttgagcgg ctcgccgccg
     9961 gtctgctccc gcgggcagta gcagagcgtg tagtcgctgt aatggacggc gtggatcagc
    10021 tcgccgcagc gcgagcaggc ttcaccctca cgcccgtgaa cctcgagcgg cttcggcagt
    10081 ttgtccggta gcggcagggt gagcccggat tcgtagaaat cgatcgccgc ctggaagcgg
    10141 tcgtggatcg acgcaagcaa ccgttccgct tcctccggct caaggtcggc cgcccgcttg
    10201 aacggggaga ggcgggcgct ccagaggatt tcgtcaaccc aggtgcggcc gatcccggcg
    10261 atcgtccgct ggtcgcgcag cagggagtag agcggccggt cggtggtcgt gaccaccccg
    10321 accggaaccg ggtccggcca cgcttccggc ccgagtttgg cgacatcctc gtcggtctcg
    10381 atttcgtcgg ccggcatcag tttcgcccag gcgcgttgcc gggtcccgaa ctcgcgcagc
    10441 ctcagttcgc gctctccgcc ctgatcgggt tccagccgga tcaggacccg cgaggtccgg
    10501 tccttcggcc cggcccgttt cccgtggagc tggagagtgc cggccgacat caggtgaatc
    10561 agcagcgaga gcccggagga gaaatcgatg accagcatct tgccgcgacg gcggatgccg
    10621 tcaatgcggt ccccggccag cccgtccagg ggcggcttga aggacttcat cacgttcagg
    10681 cccggcgcca tcgccgactc caccttcgcc ccggcaaccg cctggtcgag cagccttgcg
    10741 gtgatctcga cctcgggcag ctcgggcatc ggtcaggtcg ccgcgtctcc gtcgggagcc
    10801 gaaccgcgct ctggagccgg gggactgccg gctatcgggc gggcgatgcc gctgacgatc
    10861 gggcgggcaa ccggcggcat cgggaacttg tcctgctcgg cggtgacctc gacctcgaac
    10921 ccgttcgcct ccagcatcgg caccgtccgg cggttgcagt ggcacccgtc ggcgatcttc
    10981 atccaggtct tttccatgcg gtcctggagc ggaccgagcc gggggccctc ggagcgcacg
    11041 tgctccatga agaggaagcg gccgtcgggc ttcagcaccc ggcggatctc ggccgcggtc
    11101 gcctccgggt cggccaccgt gcacaggacc agggtttcga ccaccgtgtc gaaggtgtcg
    11161 tcctcgaagg gcaacatctc gccgggcgcc cgcacgatct cgggctccac cccggccggc
    11221 gcgggcttgg tggccaggat cccggccttg tggaaatccg gctcgaccag gaccagccgg
    11281 tcgagaccgg ccgggtaatg aaccaggttc agcccggtcc cggccccgag ctcgagcacc
    11341 gatccggaaa ccccggaaac gaccttctgg cgccagcggc gaaacccggt cttctcaccg
    11401 agccagagga acggttcgta gagacgggcc gagagcttgc tgccgggaac ctggtcggcc
    11461 gccgcttcgg cctgctgcct ggtcgcggtc aatcttcctc cgggtgttcg gcgatccagt
    11521 cccggacgaa ggggcacctg gggatgatcg agagctcgcg cgagcgggcg tcggtgaccg
    11581 cgaactcgat cagccgggat ccgaggcccc ggccgccgcg ctccgggtcg acctcggtgt
    11641 gggtgaaggt gatcacgtcc ccgtggagcc tgtattcggc gaagccggcg agctcgccgg
    11701 cctcccggat ttcatatcgg ctggcgtctg gctggtcggt gacttcgacg ttgtcttctg
    11761 aggtttccgg catggtccaa tgttagagag gtggatttct gtttcgaccg ggcggctggt
    11821 ggttaaatga tcttgaggca tgaccgtctt cgaaccgacg ctttcgagcg aaaagctcac
    11881 cgaggcgacc ggcaagaact gggacgagtg gctcgagttg ctcgaccagg ccggcgcgat
    11941 caagcggacc cacaccgaga tcgcccgctg gctgaccgat gagcagggcg tccccggctg
    12001 gtgggcccag tcgatcacgg tcggatacga aagggcccgc ggcctgaggg agccgggcca
    12061 gcgagccgac ggctggtcgg taacggcctc aaagacgatc gaggttccgg tcgaagagct
    12121 gttcgaagcc ttcgagcggg acacgatccg ggagaagtgg ctgcccgacg ccgagcttca
    12181 cctgcgcacg gcgaccgccc cggtttcggc ccgctacgac tggggtgacg gaaccacccg
    12241 gctgatcgtc gggttcgaga gcctcggccc gaaccggagc aaggtcgccc tggagcacga
    12301 gaagctgccg gatggcgagg tcgccgagag gatgaaggtc tggtggcggg agcgggtcgt
    12361 cgacctgaag gaactgctcg aagccggttg agtccgggtc ccgcggcgcc ggagaagcag
    12421 ttcaggcgcc gggtttgacc accatcaggt aggtcgcgat cacgatcagg atcccggtca
    12481 ccgtgccgat gatcccctcg atccgggcct ggcgcatgta ctcctcggag ggagtgaact
    12541 caccggggcc ggccgcctcg acctcagcgg taatcatcgc ttcgagccgc ttgtcggcgg
    12601 ggatgaagta ggccccgttg atcgctccga tcaggaacac gatcacgaag gtggcgctga
    12661 tccagaagga gccgaagtcc cagccggcgt cggtcacctg gtagatgccg gtgacgatga
    12721 tcaggagcag ggccgggctg gcgacatagc ggttgatggt cagctgaagc cggtgcacgt
    12781 agggcaggtg ccgcgggtcg agcttcatcg cgaccgggaa gaacaccgct tcggcgaagg
    12841 tcattcccag accgaccacc accgcggtga cgtggatcca gacggacaac tgatagccgg
    12901 tcacggccag cggcaggttt tccatgcccc gaccataacc cgcccgccgg agccaatacg
    12961 attcggcgtt cccgtccccc tccccgaaag gaaacatgag aaagcccttc gccctcggcg
    13021 tgatcaccct cgccgtaacg ctttcgctcg gcctcgccgc ctgtggctcc agcagcgacg
    13081 actcgctctc caatgacgag ctgatcactc aggccgacca gatctgcacc gactacaacg
    13141 acaagctggt caccctgacc gacaatgcag gccttgacaa caactcgagc aagcaggaag
    13201 tcgtcgcctt catcagtgac gaaatcgtcc cgctctacga gaaccaggtt gacgagctgc
    13261 gtgccctcga gcccaacgag gatgacgctg acgcctacaa cgacatcgtc accacgctgg
    13321 acaccgagct ccaggcggtc aaggacgatc ccgccgccgc aatgaagatg gacgatccgt
    13381 tccccgaggc gacggccaag gccaaggact tcggcctgac cgtctgcggc gccagctgat
    13441 ccgttcccga acggaaccga tgcgttcaaa aggggccgtc cgtgaaagcg ggcggcccct
    13501 tttcctatcc tgccggcatg tacctgctct tcctcgatga gtccggcatc cccggtgagg
    13561 acggaatctt cgccctcggc ggggtcgcga tccgggccga ccagtgggcc gaggtcaagg
    13621 accgtttcga gacctgcctg gaagaagcgg gctggccggc cgacaaggag ttcaaatggt
    13681 cggaaatcag ccgcagcacg gcggccgccg acgccgccta cccggtctac gactgcctcg
    13741 ccacgctgcc gatccgcagc ctcgtgaccg tgctctacac cgaaggcgat gacccggtca
    13801 accgggagaa gttcttcgcc aaccccgacc tgatctaccg gaccgccttc accttcgtgg
    13861 ccgagcgcta ccagcggttt ctcgcccacc acgaatccca cggcgtgatc gtgctcgact
    13921 cccggcagta cgagacggac gaccgtctgc gccggtactt cgaccgcatc cacaacgagg
    13981 gaaccgactt cgccgagctc gaccgcatcg tcgatggcct gctgctcggg ccctcccact
    14041 tttcgctcgg cctccagctc gccgacctgg tcgtcgggcc gacccggacc gcgcagttca
    14101 gcggccccgg caccgccagg cactgcttca agacgctgaa gccgaccttc atgacccatc
    14161 cggcgagcgg cgccctcgaa ggtgtcggcc tcaaggtgtt tccccatcag gagacgaccc
    14221 gcgaggacga ccggctcttc cggccggcct gacccgcgcg gcaaatcacc cccatcggat
    14281 gagcggggcg ccggctttcc ggcgctagac tgatccggcc ggagaggaac cgaggagaga
    14341 ggagcacgag agtgcttgac gagaaccgcc ttgccgtcct gaatgccgtt tgcgacacga
    14401 tcgtgccgtc gcttgagcgg gacgatgacc cgaccgggtt ctgggcccgg tccgccgccg
    14461 acaccaaagc aaacgaggtc gtcgcgcaga cgatcggcga gatgcccgcg gcagaccagg
    14521 agggcatggg tctcttgctc gacagcctgg cgctgcagaa cttcccttcg ctctcgcagg
    14581 cctcgcgcga gcagatcctc gtcaacacct cgctggccag ccgcgaagcg gcggtcgggg
    14641 tcgcggcgct gacccagatg accctcttct tccactacgg cctgccgccg aatccggcct
    14701 gggagcagtt cggcttcccc ggcccggcct ctccccctcc ccaggtggaa aagacgatcc
    14761 acccgctcac cccgggtgac ggcgacgtgc tcgaagccga cgccgtgatc gtcggctccg
    14821 gcgccggtgg cggtgtgatc gccgcccgac tggccgaggc cggcatgaag gtgatcgtgc
    14881 tcgagatggg cgggtacttc aacgagtccg acttcgacca gaccgagctg aacgggttcg
    14941 cgcggatgta ctggcgcggc ggcccgacct actccgccga tttcaacatc agcatccagg
    15001 ccggaagctg cctcggcggc ggcaccctga tcaactggac caacagcctc aagccgaagc
    15061 cctgggtccg gcaggaatgg gccgacgagt acggcctgga ggacgtgaac acccccgact
    15121 tcgaccgcca catcgattcg atctgggagc gctcgaaggt aaatcacgac tgctcggagc
    15181 tgaaccagac ccagaagacc tggatcgaag cggccgaaaa gctcggctgg agctggcaca
    15241 agaccgaccg caactgggac ccggaaaagc acgacccgct ggttgccggc tacatgggct
    15301 ggggtgatca gtccggggcc aagcagtcga ccctcaagac ctacctgcag gatgccgccg
    15361 accacggcgc cgaaatcatc gtcggctgcc aggcccaaaa ggtgctggtc gaaaacggcc
    15421 gcgcggccgg ggtcgaagcg acgatcgagg gcgtcggccg gatcacggtc cgctcgcccc
    15481 gggtcgtcgt tgcgggcggc gcgctcgaat caccggcgct gctccttcgc tccgggatcg
    15541 gcggcccggc cgccggcaag tacctgcggc ttcacccctg caccctgatc ttcgccacct
    15601 acccggagga ccagcaggcc tggtggggtc cgccccacgc ggcagtggtc gacgagttcg
    15661 accagggcct cgaaggcgac ggttacggct tcctgatcga aggcgcgcag tacacgaccg
    15721 gcctcggcgg ctcggcgatc ccgttcacca ccgccgccga gcaccgcgag atcatggcca
    15781 actacgcgaa cggttcgatc acgatcaacc tgacccgcga gcgcggcggc ggccaggtgc
    15841 tgatcgacgg cgacggccag tcgatgccga tctactccct ggacgacccg cgggacatcg
    15901 ccaacatgca tttcggtctc gagcagcagg tccggctcca tcatgccgcc ggggccaatc
    15961 aggtcttcgc cctcgccacc ggcatgccgg gctggcgggt cggggatgac ttcgaagcat
    16021 tcgtcgaccg ggtccaccgg atcccgctac gggccggcgg ggcgaagctg ttcgccgccc
    16081 atcagatggg ctcctgccgg atgggcaagg atccggccga aagcgtcgcc gacccgcgtg
    16141 gcgagctgca tgacaccccg ggagtctgga tcggggacgg ctcggccttc ccgactccct
    16201 ccggcaccaa tccgatgatc acgatcatgg ctctcgcctc ccgcaccgcc gagaagatcc
    16261 tcgaggattc cgggacgagc ggccgatcag ccagcgagac cgtggaggcg ggagcatgaa
    16321 tccgaccgag gcccccgaga ccgaagccaa ccaagccgcc ggcggcggcg aggcggccgc
    16381 cccgggctgg gccgcgaaga ccggcagcca cttcatcggc ggcgagtggc gtgacccgat
    16441 ctcgaccgac acgatcgagg tgatcaactc ggtgaccgaa gaggtgatgg gcaccgttcc
    16501 ggccggctcg gccgaggacg tcgacgccgc ggtcgcggcc gcccgtgcgg ccctgcccgg
    16561 ctggaaggcg ctttcggtcc aggaccgggc cggcttctgc accctgatcg gccacggcct
    16621 gcaggaccgg gccgaggaga tcgccggcct cgtcagccag gagctcggca ccccggtcac
    16681 cgaaggcatg ctgatccagg catcgcttcc ggcgatcgac ttcacctcga tggagttcct
    16741 ggtcgaggag ctgccgtggg agcagacgat cggcaactcg aaggtgatcc gcgagccgct
    16801 cggcgtggtc ggggcgatca ccccgtggaa ttacccgatc caccagatcg cggccaaggt
    16861 cgccccggcg atcaccgccg gctgcacggt cgtgctgaag ccgagcgaga tgaccccgct
    16921 ttcggcctac ctgctgatgg aggtgatcga gtcggtcggc ctgcccgccg gcgtggtcaa
    16981 catggtcacc ggcaccggcc cggtggttgg cgaggcgatc gcctcacacc ccggtatcga
    17041 catggtctcc ttcaccggtt cgacccgggc cgggatccgc gtctccgagc tggccgcggc
    17101 ctcggtcaag ccggtcacgg tcgagctcgg cggcaagtcg gccaacgtga tcctcgatga
    17161 cgccgacttc gaggcggcgg tggtcaacgg catccagaaa tgcttcatca atggtggcca
    17221 gacctgttcg gccctgaccc ggttgctggt cccccgcgag gcgctgccgg cggtcgaggc
    17281 ggtcgccgcc gccgcggtcg aacagaccgc ggtcggtgac ccgaccgacc cgacgaccca
    17341 gatcggcccg atgagctcgg ccgtccagcg cgaccgggtc cagggttaca tcgagaaggg
    17401 gatcgccgaa ggagccaagc tcgtggccgg cggcccggaa accccggagg gcctcgaaac
    17461 cggcttcttc gtcaagccga ccgtcttctc cgaggtgacc cgggagatga cgatcgcccg
    17521 ggaggagatc ttcggcccgg tcctttcgat cctcccctac gactccgagg aggaggcgat
    17581 cgagatcgcc aacgactcca gctacggcct cgccggcggt gtctggtcat ccgacccgga
    17641 gcgggcgatc ggcgtggccc gccagatcga aaccggccag gtcgagatca acggcgccgc
    17701 cttcaacccc ctcgccccct tcggcggccg gaagctatcc ggccacggcc gcgaactagg
    17761 cccctacgga atcgaagact tcctaaccct caaggcaatc cagctctagc aacgcacccc
    17821 caggaagtca acctccccga ggcatcccca ggagggcaac ctccccgagg cacccccccg
    17881 aaccgcgaga tgcatttcat cggcgaatat cgccgaccaa acgcatctcg accactcgaa
    17941 ccgccgcttt ctgagctccc ggttgcgtcc gcccggccgg gcggttagtt tcagttcaca
    18001 gggaacaacc gaatcggggg agtcggatgc gtttgaagtc gatactcgcg ctgggcctca
    18061 ccggcctcgc tctcgcctgc gcgcagagtg ccgacgcggc ccgctttgag gtgaccgacc
    18121 tcggggtgct gccgggcggg accgattcga ccgcccaggg catctccgac accggccatg
    18181 tggtcggcca atcggccggc tacccgtacg tgtggcggcc cggcagcggc atgcagaagg
    18241 tggaggtact cgcccccgac gtcgattacg ccggccccga cataaatgac gccgacctgc
    18301 tcgcagtaac cggcccccac cccgacgagg acgccttcgg gtttctctgg cacgggttcc
    18361 tctgggcgag cgggaacgcg atcgacctcg gtgcggtcgg ggaagcaccc tgcctcgagg
    18421 gatgtgacgt cgacccgaac gaccagtcct tcgccaacgg catcaacaac ttcggcaccg
    18481 tggtcggcgc ctcgaccgtc gaaaacgctt ccggaccgac ctcgatggcc ccgaccatct
    18541 ggccgggcgg cggcaacccg gttcgcctgc cggggagcgg tggcggccgg gcccgcgaga
    18601 tcgccgacaa cggccgcatc gtcggcaccc gcggcaccca cgcgatgctc tgggaggact
    18661 cgggttcgca accgaccgac ctcggcctcc tgcccggcag caccggtgac cagtccgacg
    18721 cctacgacct gaacgaggcc ggcaccgtgg tcggcgcctc ggaaaccgcg aacggcgtca
    18781 cccgcgcctt catgtggacc ccgggggccg gcatgcagcc gatcgccact cccaacacgc
    18841 tgagcggttt ctcctccgcc aacgcgatcg gcccggacgg cacgatcgtc ggctcgacct
    18901 acaacggcac caagacggtc ggcttcattc gtgaagccgg ccagccgatc accgacctca
    18961 acaccctgat ccccgagggg accggctggg atctcggcag cgcccgagac atcaacagca
    19021 agggccagat catcggcaac ggaatcctca acggcgagag ccgggcctat ctgctcacgc
    19081 cgacccagcc cctgaaggcg acgcttcagc cggtc
//
LOCUS       DAOQKN010000022        18996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_1290_length_18996_cov_5.740457, whole genome shotgun sequence.
ACCESSION   DAOQKN010000022 DAOQKN010000000
VERSION     DAOQKN010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 18996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18996
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1290_length_18996_cov_5.740457"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1588
                     /locus_tag="PLJ64_02750"
     CDS             <1..1588
                     /locus_tag="PLJ64_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNC92444.1"
                     /translation="RVLNLWRANLLGSSSKGHEYFLKHLLGTDHAVQTPECEPDRRPE
                     DVKWRDEAPEGKLDLFMTIDFRMNGSCLYSDIVLPAATWYEKHDISSTDLHPFVHPFN
                     AAIPPPWEAKTDWDIFNLIADRFSELAVDHLGTRTDIVTAPLLHDTPEELAQPGGVVK
                     DWRLGECEPIPGKTMPKLIEVERDFTAVGEKMNALGPLVEKLGIGAKGVSWKPEIEIG
                     DLAKRNGVVREGPGAGRPAIKTDIDAAEVILALSGTTNGRIANESFKALGARTGKDLT
                     VISEEHIDQRMNFSDLRIQPRKVHASAEWSGSESRERRYSPFTSNIEHLIPFRTLSGR
                     QSFYVDHAWMLDMGEGLPAYRPPVRSAQVIRRGDERSPDDPLEVAVRYLTPHSKWSIH
                     SEFQDNLHMLTLFRGGPVMWISEKDAERVEVKDNDWLEVYNSHGAVSCRAAVSVRVPE
                     GIALFYHSQDRHVNVPISEVTGQRGGTDNSLTRISMKPTHMIGGYAQLSYGFNYYGPC
                     GTQRDQLIMIRKRQEEVRYQ"
     gene            1585..3249
                     /gene="narH"
                     /locus_tag="PLJ64_02755"
     CDS             1585..3249
                     /gene="narH"
                     /locus_tag="PLJ64_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HNC92445.1"
                     /translation="MKVRAQMGMVMNLDKCIGCHTCSVTCKNVWTSRRGMEYVWFNNV
                     ETKPGRGFPVDYEDQSKWKGGWKLDRRGRLKLRAGGPLHKLLNIFSNPDLPELDDYYD
                     PWTYDYDKLISSPSSEHQPVARPKSQVTGDDLDIQWGPNWEDDLAGSAEHAAFDPNFR
                     NIQEEVKQGFEDAFMMYLPRICEHCVNPSCVASCPSGAMYKREEDGIVLVDQDACRSW
                     RFCVSGCPYKKVYFNWNTGKAEKCNFCYPRIEAGMPTVCSETCVGRLRHLGVVLIDTD
                     RVEAAASVENEHDLLNAQLDLMLDPDDPEVREQALRDGIPADWIDAASYSPVYAMAKK
                     WKIALPLHPEYRTLPMVWYVPPLSPMESVGKALVRDGEDADAENVFAAIDEMRIPVEY
                     LASMLSAGDVEPVRNSLQRLVAMRRYMRDLNLGNEARPEIATEAGMEPHELEAMYRMV
                     AIADYDDRYVIPKRHGEVSGDAFIDQGSCGIDFANGAPVPGMGEMAGVGAPDPFTGAL
                     AAARDGEVPETGALAPEQPDPKFDVRDLLTRHGGQPGSAGAGSGNV"
     gene            3242..3898
                     /gene="narJ"
                     /locus_tag="PLJ64_02760"
     CDS             3242..3898
                     /gene="narJ"
                     /locus_tag="PLJ64_02760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HNC92446.1"
                     /translation="MSEQVHPLKVVSLLLQYPEPELVDALRSVDPKEVGPASGSQRAT
                     LGGFLDWYRSQDLAELRQSYVDNFDFDRRHSLHLTYQLYGDSRQRGLALLKIKNVYRD
                     ADLELSEGELPDYLPLMLEFATLAPGQEGGDLLDRHRESIELIRAGLIRSGSPWASLF
                     DVIRDGMPGLSRRQINRIKRLAEEGPPSEEVGMEPFAPPEVMPSQPGEVPLPMIGGRS
                     "
     gene            3895..4716
                     /gene="narI"
                     /locus_tag="PLJ64_02765"
     CDS             3895..4716
                     /gene="narI"
                     /locus_tag="PLJ64_02765"
                     /inference="COORDINATES: protein motif:HMM:NF014695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HNC92447.1"
                     /translation="MSLIATSAPATAVYVVLPYVALAIGIGGLIWRRKNDQFGWTARS
                     TELLEGKVLRFASVVFHVGVLAAIGGHVLGILIPASWTDAVGISEDAYHVLAVAGGIS
                     AGIAVLVGFVALMYRRFTNPRVRVTTTNMDLLIFGLLAVGIATGLIATLVNIPDTVNY
                     RESVAPYFRQIFIFQPDPSLMTGEGVATIFQIHVIAVWFLYAAWPFSRLVHAFTVPIN
                     YPRRSPIIYRPRKPQVAAAKSKSYGSGNLDYGPSIPSGPKPTGTTGGRSRAAQGE"
     gene            4763..5959
                     /locus_tag="PLJ64_02770"
     CDS             4763..5959
                     /locus_tag="PLJ64_02770"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HNC92448.1"
                     /translation="METELKPGAGRNLALATVGFALCFAAWGFLAPLAPSFKDPPLNL
                     SSLQTSIAIAIPVLLGSLLRIPLGILTDRIGGRKVFTGMLIYSAAAAALVGFAAGSSS
                     YAAILGAGFLLGAAGASFAVGIPFVARWYPPQKQGFALGIYGMGNIGTAVAAFSIPAI
                     RDATSEGVAGLVFAAVIGVYALIWMSMAREAPVEKAPPTRYREVLGAGWKLWRLALFY
                     FVTFGGFVAMALYLPKLLVDWYDLSLTDAGLRAAGFTLLATAIRPVGGMLSDRIGGNR
                     VLAVAFLGIGIDAVGLSWQASVPTMWLTTVFCLTMALFLGLGNGAVFKLVPLTFPNAT
                     GAATGIVGAVGGLGGFFPPLFLGIVKDATGDFVLAFVMLVAFAWLCAGLAFEGRVPDS
                     GTNGPD"
     gene            complement(6042..6674)
                     /locus_tag="PLJ64_02775"
     CDS             complement(6042..6674)
                     /locus_tag="PLJ64_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNC92449.1"
                     /translation="MSQIKMVISDFGGVLTTPLVEAFASVQDRVGIPPKTFGEALRAL
                     NEREGTNPLFELECGRITEKEFQVQLADGLEPLLGHRPEIEDFGLLLFEALDPNPGMI
                     DLIREVRRDGIRTSLLTNNVKEWEVKWRSMMPIDELFETVVDSAFVGCRKPDPRIYNL
                     TLERVGLDPEECIFIDDMKINIDAANELGLHGVHFRETAQVRAEVHDLLA"
     gene            complement(6671..7414)
                     /locus_tag="PLJ64_02780"
     CDS             complement(6671..7414)
                     /locus_tag="PLJ64_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92450.1"
                     /translation="MKTRSIEILDPESGLFVSLTSGGNGAIMLGEDVVAAAPIPANGL
                     DPLVDGSPLELETEHGELAVSIESTGEPISFDGELTGRREASLVKAQGRLVHRDQEIG
                     LDCRGVLHRREEDAPETAGLSRDATIILADGGLICIAAAAPEGDFDHGDEETVAAITH
                     PGGYIEFDEVLLSTEYDTAGRQRRATLELWPATDEVAALHGAGSVIAGCTAKVGGSTI
                     NTALFRWSLDGHLGLGRYEITRPAAVAVA"
     gene            complement(7386..7931)
                     /locus_tag="PLJ64_02785"
     CDS             complement(7386..7931)
                     /locus_tag="PLJ64_02785"
                     /inference="COORDINATES: protein motif:HMM:NF016102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HNC92451.1"
                     /translation="MFVLLLALLIIWPIAELFVMVQVADWIGFFWMLFLLFASTVAGI
                     MILKHRGRAHWRRFRGAIDERRPPAKEAFDGVMITAGALLLIIPGFITSAIGLFLLFP
                     PTRWLVRLVALTLFASKFKVAATTATWSTRGYNYYRGSRQGSYDIDGEAVDVTDRPDG
                     EPLPQLRSGSECGEDQIDRNP"
     gene            8076..8393
                     /locus_tag="PLJ64_02790"
     CDS             8076..8393
                     /locus_tag="PLJ64_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HNC92452.1"
                     /translation="MAEDHATMPTEEEVFEALEEVIDPELGLDFVSLGLVYDVALEAP
                     DVYVTFTLTTPACPIGPQVSEQMKEFVGNLDGVRDVHPKMIFDPPWSPEMMTDDAKFA
                     LGF"
     gene            complement(8390..9931)
                     /locus_tag="PLJ64_02795"
     CDS             complement(8390..9931)
                     /locus_tag="PLJ64_02795"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HNC92453.1"
                     /translation="MEPASHELDRLTAIDAQFLTNEHENAHMHIGAVMIFEGDPPTYD
                     EFKAHIGSRLDFVPRYRQKLVYPPYGTGRPLWADDPDFNLNYHLRHTALPRPGGEREL
                     KILASRLFSQALDRSKPLWEMFLVEDLEGDRFAIIIKSHHAMIDGISGVDIGTVIFDV
                     TADAEPIVPKEEWKPKPLPSNLSLLERGAKELATEPVKLGEKAINALRKPSSLAGRVG
                     ESAEGLAEVARALADSAPDVPLNVPIGPHRRITWTSADLADYRRVKDVFGTTVNDVVL
                     AVAAGSIRKWLVKRGVDTDGLELRAMVPVSVRSDDERGQLGNKLATMRAPLPVYEADP
                     VARLEIVSAAMDGLKQSKQALGAEVIARLNDFAPPTLLAQAARLTFSTRLFNLLVTNV
                     PGPQIPLYVLGRKLDRPYPVAFLAKNHGLAVGVMSYAGQVNIGLIGDFDAMPDIGQVR
                     RGLNESLAELLAAAEEVENSIAPDKPGKASARKGSARKGSPGKATASNGGGPGRAAKK
                     SSPKG"
     gene            9982..10953
                     /locus_tag="PLJ64_02800"
     CDS             9982..10953
                     /locus_tag="PLJ64_02800"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNC92454.1"
                     /translation="MPETPSTGIEKVILAAPRGYCAGVDRAVQTVERALDNHGAPIYV
                     RKEIVHNKHVVEQLAARGAIFVEEETEVPEGEIVVFSAHGVSPAVHERAAARELRTID
                     ATCPLVTKVHVEARKFAAEGYTIIMVGHEGHEEVEGTMGEAPDSIVLVQTVDEVDHLE
                     IEDPDHVALITQTTLSVDETAGIIARLRERFPGIVTAKGEDICYATTNRQIAVKQLAR
                     QCDLVLVIGSTNSSNSNRLVEVAREHGSESFLIDNHTQVQEEWLDGVETVGITSGASA
                     PEELVEDLVQLFRDRGVEDVSELRTVDESVRFMLPKEIRKGLPMVNS"
     gene            complement(10960..11811)
                     /locus_tag="PLJ64_02805"
     CDS             complement(10960..11811)
                     /locus_tag="PLJ64_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92455.1"
                     /translation="MTEGGSYPYDHAILPLRDDIAAPELPGKATWIGKDPGKMPVLTA
                     KGPVLVHFLDYSQLNSVRTLPYLVEWHERYEPLGLRVLGVQAPRFPFGADPEAAGRGL
                     RQLGVEFPVLIDESKRLWTGYGCEGWPSLFLWGRGGILRWFHFGEGDYQGTEQAIQEA
                     LAASFERLPEMPAIMEPVRPTDAAGVEVSPPSPEVIPFPDRPLTIDDFDALALDFEGG
                     GAYVTADGKGDLRVTLDGEARGEVGIDGPGLYPLVETGRHGSHKLGIELEGAPEIWSV
                     SYSPAAT"
     gene            11844..12473
                     /locus_tag="PLJ64_02810"
     CDS             11844..12473
                     /locus_tag="PLJ64_02810"
                     /inference="COORDINATES: protein motif:HMM:NF015057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HNC92456.1"
                     /translation="MNTNLWDRIEQARAEHNVLEHPFYQRWSAGELTREELADYSGQY
                     RYATEAIAKLSTDIAAQAPESERAGLEAHAAEEHDHVVMWDGFVGAVGGTVGAEPNPE
                     TLACVEAWTKADGFGRQLARMFAIESGQPAISKTKTEGLGEHYEITDSPGNLYFTVHE
                     EMDVHHAAEGRKLIERYMNDFSEDELVEAAEEAFKANWLLLDGVEKARA"
     gene            12629..13864
                     /locus_tag="PLJ64_02815"
     CDS             12629..13864
                     /locus_tag="PLJ64_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="fenitrothion hydrolase"
                     /protein_id="HNC92457.1"
                     /translation="MAPGFGRAITSLPVRLLCGAFGLFLLGVAIYSGFKGTEAPDRNV
                     TLTLLFVTAWLGFPLLGVLVGNLFPAFNPWNAVAAPAGWAWRRVAGKAPAHLAYPERL
                     GRWPAVIGLFLFVWMELIYGEGSGVAVGVSPEAVAQAAVFYSIYTLVMTGLFGREAWF
                     RNGEIFSVYFGMFGSLGKFEVRGRELGIRRFLSGATTWPAGIAGSVALVITSIGTTTF
                     DGASEGVFKDGIQWLIDRFADIGFNPTAATRTSMTVFMLLSVTIVALVYLGGVRGMST
                     VPGAPDRPTLWRSFGHVLIPIAFAYLLAHYFSLFFFQEQAQFTYLLSDPLGTGTTDLF
                     GTASYGVNYNAISNQVIWYVQVAALVCGHVAGLVLAHDKAITIWKDYRTAARSQYWML
                     AVMVAFTCFGLFLLSVSNS"
     gene            complement(14348..16351)
                     /locus_tag="PLJ64_02820"
     CDS             complement(14348..16351)
                     /locus_tag="PLJ64_02820"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HNC92458.1"
                     /translation="MGEIGGALNRSVSRSMALALFGIAALFAGDDEAPASTQNLNSTC
                     GNTKVSTACSDTVYGALHNNGEAKVGPRRLVVNQSGKIFSRGTRLFVAHTPLDIGSET
                     VRIKKTDGKAKVSVAICSTAAADPFTQRVESFFTIPSGKGSIGTTFSRTVSGIKNRRL
                     SVRFAGKSLTDNFKYTMTVDRPGDDEIWMPSQTQLNSVYRPVEGFADLHAHHAGGLAF
                     ASGWYIGGMEQGSIYSGDHQKKLKVNLVGVPLTTLRPAHQGKVIPSSDDNSHQQMNLN
                     ALKAAHDNGLGLMVSPAVNSEWLCSVLAYALEINKRQSCQDMESAKYQILKMKQFDEK
                     YGWYDIVTNPWEARSAAYAGRLPVVLGVEVSDLFPKSDGDWRRQLAELYDMGVRVVYV
                     AHESNSRFAGAAYHHTEDLLLPNELKAWFSKEIKFASMKNGRNAIGLSDLGEDLIREL
                     MRRRMLIDVDHASWKAVDEIFDITSANRYYPIYAGHTRPYELLTDYMKYLVPELNTPP
                     RVLDYIKRSGGMVALRTGPEGMETYSESGVANNCSGSVRSLIQMYRYFVDEDVPVAFG
                     SDFNGFVEEIGSRFGRTGCPASTGEFRELQLTTQTRPSSAGAPPSWQRYLDRGMSDIG
                     TEPAVVFDMQKLGVDTAPLETSAQAFLQMWGRAYRSNRTEVQP"
     gene            complement(16394..16999)
                     /locus_tag="PLJ64_02825"
     CDS             complement(16394..16999)
                     /locus_tag="PLJ64_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC92459.1"
                     /translation="MSPRRSKAEAASTHEAIVREAAEGASEVGLEGISIGSLAATLGM
                     SKAGVAGHFRSKQELQLEVIEYGARDFTDKIWVPNAGLSPGRERLLGCLDTWYAYSAG
                     SPFRGGCLMTSISTEFDGRPGPVREAIAKSWGGWTRVLRADLDLAIENGELSPPLAPA
                     DTITAIESLATGVNQQTQLFGNRHPEKTASAAARVLLGILS"
     gene            complement(17026..17817)
                     /locus_tag="PLJ64_02830"
     CDS             complement(17026..17817)
                     /locus_tag="PLJ64_02830"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNC92460.1"
                     /translation="MRIRRLGWAGIEVESGGETVVVDHVLGWKSIEPYIGPPRTPLLE
                     PESAGRSLAAFVTHLHTDHTDAAAVSQTLAEGGSLHRPAVMEGEPLETGGVMAAELEL
                     KEAAVDQVLMDEWESVEVGPFVATAVPAVDGFGDPQISWVIEADGKRIIHAGDTLFHG
                     SWWLIQMRFGPFDAAFLPVNGAITSFPHRQPASGLEASLSPEQAVAAARILGAKRLVP
                     IHYDGIDEPPVYQQTDDIISRLQNTLDGEESPELVVMAEGETLDL"
     gene            17960..18475
                     /locus_tag="PLJ64_02835"
     CDS             17960..18475
                     /locus_tag="PLJ64_02835"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HNC92461.1"
                     /translation="MDFANNPAGISVSVVTVSDRCFSGEREDGSGPAAVSTLNAAGFA
                     TEGPTVVPDGEESVGSAVKKEIESGADVIVTLGGTGAGERDLTPEGTRPLIERELPGI
                     AEGLRATSAARVPTAVLSRGISGISQATASGHRSVIVNLPGSTGGARDGVQYLTPILP
                     HLIDQLRGGDH"
     gene            18475..18906
                     /locus_tag="PLJ64_02840"
     CDS             18475..18906
                     /locus_tag="PLJ64_02840"
                     /inference="COORDINATES: protein motif:HMM:NF014448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HNC92462.1"
                     /translation="MDGQATVALARISDEDLDLNRHLEAVSAPGHGAVVTFVGQIRDH
                     DPEAAGSVELVEYSAHPDAQAILERVSGEASRPGTSIAVSHRTGPVEVGQPALIACVA
                     AAHRGLAYEVSRDLVERIKRELPIWKRQVDTEGRHNWQGLR"
BASE COUNT         3206 a         6319 c         6149 g         3322 t
ORIGIN      
        1 gcgggtcctc aacctctggc gggccaacct gctcggttca tcgagcaagg gtcacgagta
       61 cttcctcaag cacctgctcg gtaccgatca cgcggtgcag accccggaat gtgaaccgga
      121 ccgccgcccc gaggacgtga agtggcggga cgaagccccc gaaggcaagc tcgacctgtt
      181 catgacgatc gacttccgca tgaacggctc ctgcctctac tcggacatcg tgctgccggc
      241 cgcgacctgg tacgagaaac acgacatctc ctcgaccgac ctgcatccct tcgtccaccc
      301 gttcaatgcg gcgatcccgc cgccctggga ggcgaagacc gactgggaca tcttcaacct
      361 gatcgccgac cgcttctcgg agctggccgt cgaccacctc ggcacgagga ccgacatcgt
      421 caccgcgcca ctgctccacg acacgccgga ggaactggcg cagccgggcg gcgtggtcaa
      481 ggactggcga ctgggcgaat gcgaaccgat cccgggcaag acgatgccga agctgatcga
      541 ggtcgaacgc gacttcaccg cggtcgggga aaagatgaac gcgctcggcc cgctggtcga
      601 gaagctcggc atcggcgcca agggtgtcag ctggaagccg gagatcgaga tcggtgatct
      661 ggccaagcgg aacggcgtcg tgcgggaagg gcccggcgcg ggtcgcccgg cgatcaagac
      721 cgacatcgac gcggccgagg tgatcctcgc cctgtccggc acgaccaacg gccggatcgc
      781 caacgagtcc ttcaaggcgc tcggggcaag gaccggcaag gacctgaccg tgatctccga
      841 ggagcacatc gaccagcgaa tgaacttcag cgacctgcgg attcagccgc gcaaggtcca
      901 tgcctcggcc gaatggtcag gctcggaatc tcgtgagcgc cgctactcgc cgttcacctc
      961 gaacatcgag cacctgatcc cgttccggac cctgagcggc aggcagagct tctacgtgga
     1021 tcacgcctgg atgctggaca tgggcgaggg cctgccggcc taccgtccgc cagtccgctc
     1081 ggcccaggtg atccggcgcg gcgacgagcg gtcaccggac gatccgctgg aggtcgcagt
     1141 ccggtacctg accccgcact cgaaatggtc gatccactcc gagttccagg acaacctcca
     1201 catgctgacc ctgttccgcg gtggcccggt gatgtggatc tcggagaagg acgccgagcg
     1261 ggtcgaggtc aaggacaacg actggctcga ggtctacaac tcccacggcg ccgtctcctg
     1321 ccgggccgcg gtctcggtgc gggtgcccga ggggatcgcg ctcttctacc actcgcagga
     1381 ccgtcacgtg aacgtgccga tttcggaggt gaccggtcag cgcggaggca ccgacaactc
     1441 actgacccgg atttcgatga aaccgaccca catgatcggg ggctacgccc agctctccta
     1501 cgggttcaac tactacggcc cttgcgggac acagcgtgac caactgatca tgatccgcaa
     1561 acgccaggag gaggttcgct accagtgaag gtccgggccc agatgggcat ggtcatgaac
     1621 ctcgacaagt gcatcggctg ccacacctgt tcggtgacct gcaagaacgt gtggaccagc
     1681 cggcgcggca tggagtacgt ctggttcaac aacgttgaaa ccaagccggg tcgtggcttt
     1741 cccgtcgact acgaagacca gtcgaaatgg aagggcggct ggaagctgga ccgccgtggc
     1801 cggctcaagc tgagggcagg ggggccgctt cacaagctgc tcaacatctt ctccaacccg
     1861 gacctgccgg aacttgacga ttactacgac ccctggacct acgactacga caagctgatc
     1921 tcctcgcctt cgagcgagca ccagccggtt gccaggccga agtcgcaggt aaccggtgac
     1981 gacctcgaca tccagtgggg tccgaactgg gaagacgacc tcgccggctc ggccgaacac
     2041 gccgcctttg acccgaactt ccgcaacatc caggaagagg tcaagcaggg cttcgaggac
     2101 gctttcatga tgtacctgcc gcggatctgc gagcactgcg tcaacccgtc ctgcgtcgcc
     2161 tcctgcccct ccggcgcgat gtacaagcgc gaggaggacg ggatcgtcct ggtcgaccag
     2221 gacgcctgcc gctcctggcg gttctgcgtc tccggctgcc cctacaagaa ggtctacttc
     2281 aactggaaca cgggcaaggc cgagaagtgc aacttctgtt acccgaggat cgaggccggc
     2341 atgccgaccg tctgctcgga aacctgcgtc ggccggctgc gtcacctggg cgtcgtcctg
     2401 atcgacaccg accgggtcga ggcggctgcc tcggtcgaga acgagcatga cctgctcaac
     2461 gcccaactcg acctgatgct ggatccggat gaccccgagg tccgcgagca ggcactgcgg
     2521 gacggcatcc cggccgactg gatcgacgcc gcttcgtact cgcccgtcta cgcgatggcg
     2581 aagaagtgga agatcgccct gccgctccac cccgagtacc ggacgctgcc gatggtctgg
     2641 tacgtgccgc cgctctcacc gatggaatcg gtcggcaagg cgctggtccg tgatggcgaa
     2701 gacgccgacg ccgaaaatgt cttcgcggcg attgacgaga tgcggatccc ggtcgagtac
     2761 ctggcttcga tgctctcggc cggcgatgtc gagccggtcc gcaattcact tcagcggctg
     2821 gtcgcgatgc ggcggtacat gcgtgatctc aacctcggca acgaggcccg gcccgagatc
     2881 gcaaccgagg ccggcatgga gccgcatgag ctcgaggcga tgtaccggat ggtggcgatc
     2941 gccgattacg acgaccggta cgtgatcccg aagcgtcacg gcgaggtttc cggggacgct
     3001 ttcatcgatc agggatcctg cgggatcgat ttcgccaacg gggcaccggt ccctggcatg
     3061 ggagagatgg ccggagtcgg cgcaccggac ccgttcacgg gtgcgctggc cgccgcgcgg
     3121 gacggtgaag tcccggaaac cggcgcgctt gcccccgaac agcccgaccc gaagttcgac
     3181 gtgcgcgacc tgctgaccag gcatggtggc cagcccggtt cggccggagc gggaagcggc
     3241 aatgtctgag caggttcatc cgctgaaggt cgtctcgctg ctcctgcagt acccggagcc
     3301 cgaactggtc gacgcgctgc ggagcgtgga tccgaaagag gtcggtcccg cgtcgggctc
     3361 ccagcgcgcg acgctgggcg ggttcctcga ctggtaccgc tcgcaggacc tcgcggagct
     3421 ccgccagtcc tacgtcgaca acttcgactt cgaccggcgt cacagccttc atctcaccta
     3481 ccagctctat ggcgacagcc ggcagcgcgg cctggccctg ctcaagatca agaacgtcta
     3541 ccgcgacgcc gacctcgagc tttcagaagg cgagctgccg gactacctgc cgctgatgct
     3601 cgagttcgcg accctggcgc ccggccagga aggcggggac ttgcttgacc ggcaccgcga
     3661 atcgatcgaa ctgatccggg cgggcctgat ccgctcggga agcccttggg cgtcgctctt
     3721 cgacgtgatc cgggacggga tgccgggcct ctcccggcgc cagatcaacc ggatcaagcg
     3781 gctggccgaa gaaggcccgc cgagcgagga ggtcggcatg gagccgttcg cgccgcccga
     3841 agtcatgccc agccaaccgg gggaggttcc cctgccgatg atcggaggcc gaagttgagt
     3901 ctgatcgcga ccagtgcccc ggccaccgcg gtttatgtcg tcctgcccta cgtggctctc
     3961 gcgatcggga tcggcggcct gatctggcgc cgcaagaacg accagttcgg ctggaccgcc
     4021 cgctcgaccg agttgctcga aggcaaggta ctcaggttcg ccagtgtggt cttccacgtc
     4081 ggcgtgctgg ccgcaatcgg cggccacgtg ctcggcatcc tgatccccgc ttcctggacc
     4141 gacgcggtcg gcatttccga agacgcctac cacgtgctgg ctgtggccgg cggcatctcg
     4201 gcgggcatcg ccgtcctggt cggattcgtc gccctgatgt accgccgctt caccaacccg
     4261 cgggtaaggg tgaccacgac caacatggac ctgctgatct tcggcctcct tgcggtcggg
     4321 atcgccacgg gacttatcgc gaccctggtc aacatccccg acacggtcaa ttaccgcgag
     4381 tcggtcgccc cctacttccg ccagatattc atcttccagc cagatccttc gctgatgacc
     4441 ggcgagggag tcgcgacgat cttccagatc catgtgatcg cggtctggtt cctttacgca
     4501 gcctggcctt tcagccggct tgttcacgcg ttcaccgttc cgatcaacta cccccgccgc
     4561 agcccgatca tctaccgccc gcgcaagccg caggtggcag ccgccaagtc aaagagctac
     4621 ggcagcggaa acctcgacta cggacccagc atcccctccg gccccaaacc aactggaacg
     4681 accggcggtc gttcgcgggc cgcgcaaggc gagtagccgc gactcaaccc tctttccact
     4741 aacacaccaa caggagaatc cgatggaaac tgaactcaaa cccggtgcag ggcgcaacct
     4801 ggctctcgcc acggttggat tcgcgctctg cttcgcggcc tgggggttcc ttgccccgct
     4861 cgcgccgtcg ttcaaggatc cgccgctcaa cctttccagc ctgcagacct cgatcgcgat
     4921 cgccatcccc gtcctgctcg gctcgctgct ccgcatcccg ctcggcatcc tcaccgaccg
     4981 gatcggtggc cgcaaggtct tcaccgggat gctgatctac tcggccgcag ccgcggcact
     5041 ggtcggattc gctgccgggt catcgtccta cgcggccatt ctcggcgccg gcttcctgct
     5101 cggcgccgcc ggagcatcct tcgcggtcgg cattccgttc gtcgcccgct ggtacccgcc
     5161 gcagaagcag ggcttcgcgc tcggcatcta cggcatgggc aacatcggca ccgcggttgc
     5221 cgccttctcg attccggcga tccgcgacgc caccagtgaa ggtgtggcgg gactcgtctt
     5281 cgccgcagtg atcggcgtct acgcgctgat ctggatgtcg atggcccggg aggcaccggt
     5341 cgaaaaggcg ccgccgaccc gctaccgcga ggtgctcggc gccggctgga aactctggcg
     5401 actggcgctc ttttacttcg tgaccttcgg tggattcgtc gccatggcgc tatacctgcc
     5461 gaagctgctg gtcgactggt atgacctttc gctcaccgac gccgggttgc gcgctgccgg
     5521 cttcaccctg ctggcaaccg cgatcaggcc ggtcggcggc atgctctcgg accggatcgg
     5581 tggcaaccgc gtactggcgg tcgccttcct cgggatcggc atcgacgcgg tcggcctctc
     5641 ctggcaggcc tcggttccga cgatgtggct gaccaccgtc ttctgcctga ccatggccct
     5701 cttcctcggt ctcgggaacg gcgccgtgtt caagctggtg ccgctcactt tcccgaacgc
     5761 aaccggtgcc gcaaccggca tcgtcggcgc cgtgggtggc ctcggcggct tcttcccgcc
     5821 cctgttcctg ggaatcgtga aggacgcaac gggtgacttc gtgctcgcct tcgtgatgct
     5881 ggtcgccttc gcctggctgt gtgccggcct ggccttcgag ggccgggttc ccgactcggg
     5941 gacgaacggc ccggactagt ctgagccact tcggtacggc gcccctcccg acctccgagc
     6001 gggaggggct ccgcccgaag taaagccagg cgaaaccctg tttacgccag caggtcgtgc
     6061 acttcggccc ggacctgtgc ggtttcgcgg aagtgaaccc cgtggagccc gagttcgttc
     6121 gcggcatcga tgttgatctt catgtcgtcg atgaagatgc attcctccgg gtcgaggccg
     6181 acccgttcca gggtcaggtt gtagatccgc ggatccggct tgcggcagcc gacgaaagcc
     6241 gagtcgacca cggtctcgaa gagctcgtct atcggcatca tcgagcgcca tttgacttcc
     6301 cactccttga cgttgttggt cagaagtgaa gtgcggatgc cgtcccggcg gacctcccgg
     6361 atcaggtcga tcatgcccgg gttcgggtca agggcttcga agagcagcag gccgaagtcc
     6421 tcgatttccg gccggtggcc gagcaacggc tcgaggccat ccgccagctg gacctgaaat
     6481 tccttttcgg tgatccggcc acactcgagc tcgaagagcg gattggtgcc ctccctctcg
     6541 ttcagcgccc gcagcgcctc gccgaaggtc ttgggcggga tcccgacccg atcctgcacc
     6601 gaagcgaatg cctcgaccag cggggtcgtc agcaccccgc cgaaatccga gatgaccatc
     6661 ttgatctggc tcatgcgacc gctaccgccg cgggacgggt gatctcgtaa cggccgaggc
     6721 cgaggtggcc atcgagcgac cagcggaaga gggcggtgtt gatcgtcgag cccccgacct
     6781 tcgcggtgca gccggcgatc accgatccgg caccgtgaag cgcggccacc tcgtcggtgg
     6841 ccggccagag ctcgagggtg gcccgacgct ggcgcccggc cgtgtcgtac tcggtcgaaa
     6901 gcaggacttc atcgaattcg atgtaaccac cggggtgggt gatcgccgcc accgtctcct
     6961 cgtcaccgtg gtcgaagtca ccttccgggg cggccgcggc gatacagatc agcccgccgt
     7021 cggcaaggat gatcgtcgcg tcgcggctca ggccggcggt ttccggcgcg tcctcctcac
     7081 gccggtggag caccccgcgg caatcaaggc cgatctcctg gtcgcggtgg acgaggcgtc
     7141 cctgggcctt gaccaggctg gcctcacgcc ggccggtcaa ctcgccgtcg aagctgatcg
     7201 gctccccggt cgattcgatc gaaacggcga gttcaccatg ttcggtttcc agttcgagtg
     7261 gtgaaccgtc gacgagcggg tcgagcccgt tggccggaat cggagccgcc gcgacgacat
     7321 cttcaccgag catgatcgcg ccgttgccgc cgctggtcag gctgacgaag agtccgctct
     7381 cggggtcaag gatttcgatc gatctggtct tcaccgcact ctgacccgga gcgtagctgg
     7441 gggagaggct caccgtcggg ccggtcggtc acgtcaaccg cttcgccgtc gatgtcgtag
     7501 gaaccctggc gggagccccg gtagtagttg tagccacgag tgctccaggt cgcggtggtg
     7561 gccgcgacct tgaacttgga ggcgaagagg gtcagcgcga ccaggcgaac gagccacctg
     7621 gtcggcggga agagcaggaa aagaccgatc gccgaggtga tgaagccggg gatgatcaaa
     7681 agcagcgcgc cggcggtgat catcaccccg tcgaaggctt ccttcgctgg cggtcgtcgc
     7741 tcatcgatcg ccccgcggaa ccggcgccag tgcgctctgc cgcggtgttt gaggatcatg
     7801 atgcccgcca cggtcgaggc gaagagcagg aacagcatcc agaagaagcc gatccagtcg
     7861 gccacctgca ccatcacgaa aagttccgcg atcggccaga tgatcagcaa tgccaggagc
     7921 agtacgaaca tgggcagata caggttacgc acccgggcaa tccgggttac agcctgcggt
     7981 gggtcgggtt tccgtcaccc cggccggaaa cccgacccac cggccgaagc agcacgggct
     8041 ggcggccgga atcccgaccc agctaggatg cattcatggc tgaagaccac gcaaccatgc
     8101 cgaccgaaga agaggttttc gaagcgctcg aggaagtgat cgaccccgaa ctcgggctcg
     8161 acttcgtttc actggggctc gtctatgacg ttgcgctgga agcgccggac gtctacgtga
     8221 cattcaccct gaccacgccc gcctgcccga tcggacccca ggtctccgag cagatgaagg
     8281 aattcgtcgg caacctcgac ggcgtccgcg acgttcaccc gaagatgatc ttcgacccgc
     8341 cctggtcgcc ggaaatgatg accgacgacg ccaagttcgc gctcggcttc tagcccttcg
     8401 ggctgctctt cttcgccgcc cggcccgggc cgccgccgtt gctggcagtg gccttcccgg
     8461 ggctgccctt ccgggctgaa ccctttctgg ccgaagcctt cccgggtttg tcgggggcga
     8521 tcgagttctc gacctcttcg gcggcggcga gcagctcggc cagcgattcg ttcaggccgc
     8581 ggcggacctg gccgatgtcg ggcatcgcgt cgaagtcacc gatcaggccg atgttgacct
     8641 gcccggcgta actcatcacg ccgaccgcaa ggccgtggtt cttggcgagg aaggcgaccg
     8701 gatagggccg gtcgagtttg cgtcccagga catagagcgg aatctgcgga cctggaacgt
     8761 tggtcacgag cagattgaaa agccgggtgg agaaggtcag ccgggccgcc tgcgcgagca
     8821 gggtgggcgg ggcgaagtca ttgagtcggg caatcacttc cgcccccagc gcctgcttgg
     8881 actgcttcag gccgtccatc gcggcgctga cgatctccag ccgggcgacc ggatcggcct
     8941 cgtaaaccgg cagcggggcc cgcatcgtgg cgagcttgtt gccgagctgg ccgcgctcgt
     9001 cgtcggagcg gaccgagacc ggaaccatgg cccgcagctc aaggccatcg gtgtcaaccc
     9061 cgcgcttgac cagccacttg cgaatcgaac cggcggcgac cgccagcacc acgtcgttga
     9121 cggtggtgcc gaaaacgtcc ttgacccgcc ggtagtcggc caggtcggcg ctggtccagg
     9181 tgatccggcg atggggaccg atcggcacgt tcaacggcac gtcgggcgcg gaatcggcca
     9241 gagcccgggc cacctcggcc agtccctcgg cactttcccc gacccgcccg gccagcgacg
     9301 aaggcttgcg caaggcgttg atcgccttct cgcccagctt caccggttcg gtcgcgagtt
     9361 ccttcgcgcc ccgttccagc aggctcaaat tcgagggcag cggcttcggc ttccattcct
     9421 ccttggggac gatcggctcg gcgtcggcag tgacgtcaaa gatcaccgtg ccgatgtcaa
     9481 ccccggagat gccgtcgatc atcgcgtggt ggctcttgat gatgatcgcg aaccggtcgc
     9541 cctccagatc ctcgaccagg aacatttccc agagcggctt ggaacggtcc agggcctgcg
     9601 agaagagccg cgaggcgaga atcttcagct cgcgttcgcc acccggcctc ggcagggcgg
     9661 tgtggcgcag gtggtagttc aggttgaaat caggatcatc ggcccagagc ggacggccgg
     9721 tgccgtaagg cggatagacc agcttctggc ggtacctggg cacgaaatcg agccgcgagc
     9781 cgatgtgggc cttgaactcg tcataggtcg gcggatcccc ttcgaagatc atcaccgcac
     9841 cgatgtgcat gtgagcgttt tcgtgctcgt tggtcagaaa ctgcgcatca atcgcagtga
     9901 gtcggtcaag ttcgtggctg gcgggttcca tggctcccca tcgtggacta gcacaagact
     9961 ctactcgggt acccttgtcc aatgcccgaa acaccctcca ccgggataga gaaagtcatt
    10021 ctggccgcgc cccgtggcta ctgcgccggc gtcgaccggg ccgtgcagac ggtcgagcgc
    10081 gcgctcgaca accacggtgc cccgatctac gtccgcaagg aaatcgtcca caacaagcac
    10141 gtggtcgagc agctagcggc gcggggagcg atcttcgtcg aagaggaaac cgaggtgccc
    10201 gaaggcgaga tcgtcgtctt ctccgcccac ggcgtttcgc cggcggttca cgaacgggct
    10261 gcggctcggg agctgcgcac gatcgacgcg acctgcccgc tggtcaccaa ggtccacgtc
    10321 gaggcccgca agttcgcggc cgagggctac acgatcatca tggtcggcca cgagggccac
    10381 gaagaggttg aaggcacgat gggcgaggca ccggactcga tcgtcctggt ccagaccgtt
    10441 gacgaggtcg accaccttga aatcgaggat ccggaccacg tcgccctgat cacccagacc
    10501 accctttcgg tcgatgagac cgccgggatc atcgcccgcc tgcgcgaaag gttccccgga
    10561 atcgtcaccg ccaagggcga ggacatctgt tacgccacca ccaaccgtca gatcgcggtc
    10621 aagcagctgg cccgccagtg tgacctggtc ctggtgatcg gctcgaccaa ctcctcgaac
    10681 tcgaaccggc tggtcgaggt cgcgcgcgag cacggctccg aatccttcct gatcgacaac
    10741 cacacccagg ttcaggaaga gtggctcgac ggggtggaaa cggtcgggat cacttccggg
    10801 gcaagcgcac cggaggaact ggtcgaggat ctggttcagc ttttccgcga ccgcggggtg
    10861 gaggacgtct ccgagctccg caccgttgac gagtcggtcc gcttcatgct gccgaaggag
    10921 atccgcaagg gcctcccgat ggtcaattcc tgaccggtct caggtcgcgg ccggcgagta
    10981 actgaccgac cagatctccg gagcgccttc cagttcgatc cccagcttgt ggctgccgtg
    11041 gcggccggtt tcgacgagcg ggtaaagccc cgggccatcg atcccgactt cgccgcgggc
    11101 ttcgccgtcg agggtaaccc gcagatctcc cttgccatcg gccgtgacgt aggcgccacc
    11161 gccctcgaaa tcgagcgcca gcgcgtcgaa gtcatcgatc gtgagggggc ggtccggaaa
    11221 cgggatcact tcgggtgagg gtggcgacac ctcaacgccc gctgcgtcgg tgggccggac
    11281 cggttccatg atcgccggca tctccgggag cctctcgaag gaggcggcca gcgcttcctg
    11341 aatcgcctgc tcggttccct gatagtcgcc ctcgccgaag tggaaccagc ggaggattcc
    11401 cccgcgaccc cagaggaaga ggctcggcca gccttcacat ccgtaaccgg tccagagtcg
    11461 cttgctttca tcgatcagga ccgggaactc gaccccgagt tgccgcagtc cgcgcccggc
    11521 ggcttccggg tcggcgccga agggaaagcg tggagcctgg acaccgagca cccgcaggcc
    11581 gagcggctcg taacgctcgt gccactcgac caggtagggc agggtgcgca cgctgttcag
    11641 ctggctgtaa tccagaaagt ggaccagtac agggcctttt gccgtgagca ccggcatctt
    11701 gcccgggtcc ttgccgatcc aggtcgcctt gccgggcagt tcgggagccg cgatgtcatc
    11761 tcgaagaggg aggatggcgt gatcgtatgg atatgatccg ccttcagtca ccccctctac
    11821 gagctctacg aggagagaaa ctcgtgaaca cgaatctgtg ggaccgcatc gagcaggccc
    11881 gggccgagca caacgtcctg gaacatccct tctaccagcg ctggtcggcc ggcgagctga
    11941 cccgcgagga actcgccgat tactcgggcc agtaccgcta cgcgaccgag gcgatcgcca
    12001 agctgagcac cgacatcgcc gcccaggcac cggaatccga gcgggccggg ctcgaggcgc
    12061 atgcggccga agagcacgat cacgtcgtca tgtgggacgg cttcgtgggc gcggtcggcg
    12121 gcacggtagg tgccgagccg aacccggaga ccctcgcctg cgtcgaagcc tggaccaagg
    12181 ccgacggttt cggccgccag ctcgcgcgca tgttcgcgat cgagtcgggc cagccggcga
    12241 tttcaaagac caagaccgaa ggtctcggcg agcactatga gatcaccgac tctcccggca
    12301 acctctactt caccgttcac gaggagatgg acgtccatca cgccgccgag ggtcgcaagc
    12361 tgatcgagcg gtacatgaac gatttcagtg aggacgagct ggtcgaggcg gccgaggaag
    12421 ccttcaaggc caactggctg ctgctcgacg gagtcgagaa ggcgcgggcc tgagccttcc
    12481 cctcgcacat gtcctgtccg ccgggcagga cctgccaata ccggcgtggc tcttcgcctg
    12541 gggagcctcg attgttctcg tagtctcgtt cttcgctctc tcagcgggct ggcgccgggc
    12601 ccgctacgag agtgattcct ggcgtcaggt ggcgcccggt ttcggccgcg cgatcaccag
    12661 cctgccggtc cggctcctct gcggggcctt cggcctcttc ctgctcgggg tcgcgatcta
    12721 ttccggcttc aaaggcaccg aggcgccgga ccgaaatgtc accctgaccc tgcttttcgt
    12781 caccgcctgg ctcggctttc cgttgctggg agtgctggtc gggaacctct ttcccgcctt
    12841 caacccatgg aacgcggtcg ccgccccggc cggctgggcc tggcgcaggg tcgcgggcaa
    12901 ggctcctgcc caccttgcct atccggagcg gctcggacgc tggccggccg tgatcgggct
    12961 gttcctcttc gtctggatgg agttgatcta cggtgaggga agcggcgtgg cggtcggcgt
    13021 ttcacccgag gcggtcgccc aggcggcagt cttctactcg atctacaccc tggtcatgac
    13081 cggcctcttt ggccgggagg cctggttccg aaacggcgag atcttctcgg tctacttcgg
    13141 catgttcggc tcgctcggca agttcgaggt ccggggccgt gaactcggca tccggcgctt
    13201 cctttccgga gcgacaacct ggccggccgg gatcgccggc tcggtcgcac tggtcatcac
    13261 ctcgatcggc accaccactt tcgacggtgc ctcggaaggc gtcttcaagg acgggatcca
    13321 gtggctgatc gaccggttcg cggacatcgg tttcaacccg accgccgcga cccgcacctc
    13381 gatgaccgtc ttcatgcttc tcagcgtcac tatcgtggcg ctggtctacc tgggcggggt
    13441 gcggggcatg agcacggtcc ctggcgcccc cgaccggccg accctctggc ggtccttcgg
    13501 ccatgtcctg atcccgatcg ccttcgccta cctgctcgcc cactacttca gcctcttctt
    13561 cttccaggag caggcccagt tcacctatct gctctccgat ccgctcggaa ccggcaccac
    13621 cgacctcttc ggaaccgctt cctacggggt caactacaac gcgatctcca atcaggtgat
    13681 ctggtacgtc caggtcgccg ccctcgtctg cggccacgtc gccggcctcg tcctcgccca
    13741 cgacaaggcg atcaccatct ggaaggacta ccgcaccgcc gcccgctccc agtactggat
    13801 gctcgccgtg atggtcgcct tcacctgttt cgggctcttt ctactgtcag tctcgaacag
    13861 ttaaggcccg ggcaacgcgc ccaggacgca cctgcgggcg ccgtggcttc gtcaacgccg
    13921 ggaaacggca gctcaagtac ccgcgtacga tcgcggccat ttcccggctt attcctcgcc
    13981 agccacccgc aggagcgccc tgtgcacgac ccgctgacgg ctggaggccc acgacccact
    14041 cacggctgga ggtccctggc atccacccgc cgaagtttgg cccgctgacg gctggaggcc
    14101 cacgacccgc tgacggctgg tggtgaaccc gtattcgtga gatgcggttt gtcagtaaat
    14161 agcgctgaca gatcggatct cgatcgattt cccgacccgg atccggggct ggtgggtatg
    14221 gatttcgttc atatagacga aatccatacc cacctcccga aacctgggcc tggacctggc
    14281 cctggagacg gtgcgggacg ctgggacgcc aacaacttcg ccctcaatgc cgccggccgg
    14341 tttcagctca gggctggact tcggtgcggt tgctccggta ggcgcggccc cacatctgga
    14401 ggaaggcctg ggccgaggtc tcgaggggtg cggtgtcgac cccgagtttc tgcatgtcga
    14461 agacgaccgc cggttcggtg ccgatgtccg acattccccg gtcgaggtag cgctgccagc
    14521 tgggcggcgc accggcggag gaggggcggg tctgtgtggt caactgcagt tcccggaact
    14581 cgccggtcga ggccgggcag ccggtccgcc cgaacctgga tccgatctct tccacgaacc
    14641 cgttgaagtc actgccaaag gcgaccggca cgtcttcgtc gacgaagtac cggtacatct
    14701 ggatcagcga ccggaccgag cccgagcagt tgttggctac gccgctttcg ctgtaggtct
    14761 ccatcccttc gggtccggtc cgcagggcga ccatgccgcc actccgcttg atgtagtcaa
    14821 ggacccgggg cggggtgttg agctccggca cgaggtactt catgtagtcg gtaagcagct
    14881 cgtagggtcg cgtgtggccc gcgtagatcg ggtagtagcg gttggcgctg gtgatgtcga
    14941 agatttcgtc taccgccttc caggaggcgt gatcgacgtc gatcagcatg cgcctcctca
    15001 tcagctcgcg gatcaggtcc tcgccgaggt cgctgagccc gatcgcgttg cggccgttct
    15061 tcatcgaagc gaacttgatc tccttcgaga accaggcctt cagttcgttg ggaagcagca
    15121 ggtcctcggt gtggtggtag gcggcgccgg cgaaacggct gttcgactcg tgggccacgt
    15181 agacgacccg gacgcccatg tcatagagct cggcaagctg acgccgccag tccccgtcgg
    15241 acttggggaa gaggtcgctc acctcgactc ccagcaccac cggcaggcgg cccgcgtagg
    15301 ccgccgatcg cgcctcccag ggattggtga cgatgtcata ccagccgtat ttctcatcga
    15361 actgcttcat cttgaggatc tggtacttcg ccgactccat gtcctggcag gactgccgct
    15421 tgttgatctc gagcgcgtag gcgagcaccg aacagagcca ctcgctgttg acggcggggg
    15481 agaccatcaa tccgagcccg ttgtcatggg ccgccttcag cgcgttgagg ttcatttgct
    15541 gatggctgtt gtcatccgag ctcggaatca ccttcccctg atgggccggc cgcagcgtgg
    15601 taagcggaac cccgaccaga ttcaccttca gcttcttctg gtgatccccg gaataaatcg
    15661 acccctgctc catgccgccg atgtaccagc cactggcgaa ggcgagtccg ccggcatgat
    15721 gcgcgtgaag gtcggcgaag ccttcgaccg ggcgatagac cgagttgagc tgcgtctggc
    15781 tgggcatcca gatctcgtcg tcgcccgggc ggtctacggt catcgtgtac ttgaagttgt
    15841 cggtgaggct cttgccggcg aaccgcaccg agagccgccg gttcttgatg ccgctgacgg
    15901 tccgggagaa agtggtgccg atgcttccct tgccgctcgg gatggtgaag aaactctcga
    15961 cccgctgcgt gaaggggtcg gcggctgcgg tcgaacagat cgcgacgctg accttggcct
    16021 tgccgtcggt cttcttgatc cgcacggtct cggagccgat gtccagcggg gtgtgggcga
    16081 cgaagagccg ggtgcccctg gagaagatct ttcccgactg gttgacgacc aggcgacggg
    16141 gcccgacctt cgcctcgccg ttgttgtgca gcgcgccgta aaccgtgtcc gaacacgcgg
    16201 tcgagacctt cgtgttgccg caggtcgaat tgagattctg ggtacttgcc ggggcttcgt
    16261 catctcccgc gaaaagtgcc gcgatcccaa aaagtgccag cgccatcgag cggctgaccg
    16321 atcggttgag tgctccccca atttccccca tgagacgaga ctaaccgcgg acggtcgggc
    16381 tgcggggaaa ttctcaggaa aggattccga gcaggacgcg ggcggcggcg ctggcggtct
    16441 tttccgggtg gcggttgccg aagagctggg tttgctggtt gaccccggtc gccagcgact
    16501 cgattgcggt gatcgtgtca gcgggagcga gcgggggaga gagctcgccg ttttcgatcg
    16561 cgaggtcgag gtcggcccgg aggacccggg tccagccgcc ccaggatttc gcgatcgcct
    16621 cccgtaccgg gccgggccgg ccgtcgaatt cggtcgagat cgaggtcatc aggcagccac
    16681 cgcggaacgg gctgccggcc gaatacgcgt accaggtgtc gaggcagcca agcaggcgtt
    16741 cccggccggg agaaagcccg gcgttcggaa cccagatttt gtcggtgaaa tcccgcgccc
    16801 cgtactcgat cacctcgagc tgaagctcct gcttgctccg gaagtgcccg gccacgccgg
    16861 ccttgctcat cccgagcgtg gcggcaagcg agccgatcga gatcccctcc agcccgacct
    16921 cggaagcgcc ctcggccgct tcccgcacga ttgcctcatg tgtggaagcg gcttcggctt
    16981 tcgaccggcg cggactcatg gcctgactat aggaactgcc gccggtcaga ggtcaagcgt
    17041 ctcgccttcc gccatcacga ccagttccgg gctttcctcg ccgtcgagcg tgttctggag
    17101 ccgggagatg atgtcgtcgg tctgctgata gaccggcggc tcgtcgatac cgtcgtagtg
    17161 gatcgggacc agccgcttgg cgccgaggat ccgggccgcc gcgaccgcct gctccgggct
    17221 gagcgaggct tccaggccgc tggccggctg ccggtggggg aaggaggtga tcgctccgtt
    17281 gaccgggagg aaggcggcat cgaagggtcc gaaacgcatc tggatcagcc accaggaacc
    17341 gtgaaagagc gtgtcgccgg catggatgat tcgcttgccg tcggcctcga taacccagga
    17401 gatctggggg tcgccgaaac cgtcgaccgc cggcacggcg gtggccacga agggcccgac
    17461 ctcgaccgat tcccattcat ccatcaggac ctggtcgact gccgcttcct tcagttccag
    17521 ctcggctgcc atcacaccgc cggtctccag cggctcaccc tccatcactg ccggacggtg
    17581 aagcgagccg ccttcggcca gggtctgtga gacggcggcg gcatcggtgt gatcggtgtg
    17641 gaggtgcgtc acgaaggcag ccagggaccg gccagccgat tcgggctcca gcaacggggt
    17701 gcggggaggg ccgatgtagg gctcgatcga tttccagccg aggacatggt cgaccaccac
    17761 ggtctccccg cccgattcga cctcgatccc ggcccagcca agtcttctga ttcgcatctt
    17821 cttcccttca tcgctttgac gatcgcggcg gccggctcga tcccggcgac ctgacatcca
    17881 atagatagca tacgatcgtt cgtacttcaa gcgaccgggg gcgaggtggg cgaagaggca
    17941 ggctccatat gctggtttca tggacttcgc gaacaatccg gccgggattt ccgtgagtgt
    18001 ggtgacggtc tccgaccgct gcttcagcgg agagagggag gatggctccg gccccgcggc
    18061 ggtcagcact ttgaacgcgg ctggcttcgc gaccgaaggc cccaccgttg tccccgacgg
    18121 agaagaaagc gtcggttcag cggtgaagaa ggagatcgag tcgggggccg acgtgatcgt
    18181 cacgctcggc ggcaccggag cgggtgaacg ggacctgacg cccgaaggga cccgtccgct
    18241 gatcgaacgc gaactgccgg gtatagccga aggccttcgc gccacgagcg cggcccgggt
    18301 tccgaccgcg gtgctctccc ggggcatctc ggggatcagc caggccaccg catccggcca
    18361 ccgctcggtg atcgtcaatc tgccgggttc gaccggcggt gcccgggacg gagttcagta
    18421 cctcacaccg atcctgccgc acctgatcga tcaactgcgg ggcggggatc actgatggac
    18481 gggcaggcga ccgtggccct ggcccggatc agcgacgaag acctcgacct gaaccggcac
    18541 ctcgaagccg tgtccgctcc cggtcatggc gccgtggtga ccttcgtcgg gcagatccgc
    18601 gaccacgacc ccgaagcggc cggcagcgtc gagctggtgg agtactcggc tcatcccgac
    18661 gcccaggcca tcctcgagcg ggtttccggg gaagcctccc ggccgggcac gagcatcgcc
    18721 gtgagccacc ggaccgggcc ggtcgaggtc ggccagccgg cgctgatcgc ctgtgttgcc
    18781 gccgcccacc gggggctggc ctacgaggtg agccgggatc tggtcgagcg gatcaagcgg
    18841 gagctgccga tctggaaacg acaggtcgat accgaaggtc gccacaactg gcaggggctt
    18901 cgatgagcgg cctggttgac cggttcggcc gggtccaccg cgacctgcgc gtctcgctca
    18961 ccgacaagtg ctcgctgcgc tgcacctact gcatgc
//
LOCUS       DAOQKN010000023        17899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_1417_length_17899_cov_5.341852, whole genome shotgun sequence.
ACCESSION   DAOQKN010000023 DAOQKN010000000
VERSION     DAOQKN010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 17899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17899
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1417_length_17899_cov_5.341852"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="PLJ64_02845"
     CDS             <1..873
                     /locus_tag="PLJ64_02845"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNC92463.1"
                     /translation="VFGLAVIGDVEALAGIYAFGATLAITIANLSVLRLRRSMPDAER
                     PFRAPFNLKIGSVDRPLSAVIGAVLSSLALVSVLLFHDSARWVGLGWLAVGLVGYVIY
                     RRVVEQISLTKQVAVEPRALTRPRVSIDFHNILVPIFGTRIDDDIVSTAGRMAAEGDP
                     EEEHTKAVMTILYLIEIPLTRAIRDPLPEEVAEEAQRATKRAKEVADEYEGVEIRVEI
                     ERVRRKGTGIIFAARRLDVDAIVMGAEPPSPIKGGAQLGGIGDYRPEEIGPITAYVLK
                     RAPCRVLLTAPRTA"
     gene            889..1401
                     /locus_tag="PLJ64_02850"
     CDS             889..1401
                     /locus_tag="PLJ64_02850"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HNC92464.1"
                     /translation="MFVLIVGCGRVGSTVARSMLSKGHSVSCLDEDPESHARLEVGLD
                     TSWEEAGGQFTVGTALETQALEGAGIIEADAFVAATNGDNTNIVIAQIAQKRYEVETV
                     IARILDPLRAEWYREQGLRTICPTKVAVEMLEDKLLSAANPTSLDAGSAEFADSPPAP
                     PAGPATGGDE"
     gene            1402..2061
                     /locus_tag="PLJ64_02855"
     CDS             1402..2061
                     /locus_tag="PLJ64_02855"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HNC92465.1"
                     /translation="MYVIVAGAGKVGVNLTRELLGQGHEVTLIENRRQRYLGVEQLLE
                     HNILYGDASELWVLERAGIERAEMVIAVTGDDEDNILICQVAKEKYGIGRIIARVNNP
                     SNRAHFDLLGIKPVVSATELILRLLEHEVPHHNLVHLLDLEEEEIEIIEIRLGSKSPV
                     VGRSVGEVGTPDNSLVIAIIREGRGVVPNASTVFEEGDDILVVLDPEREEELKQIFTD
                     G"
     gene            complement(2105..2175)
                     /locus_tag="PLJ64_02860"
     tRNA            complement(2105..2175)
                     /locus_tag="PLJ64_02860"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2141..2143),aa:Gln,seq:ctg)
     gene            2298..2981
                     /locus_tag="PLJ64_02865"
     CDS             2298..2981
                     /locus_tag="PLJ64_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92466.1"
                     /translation="MPTTGFDSRRVLGLALISLVISAGAIGCGGGGDDETAAPVPTTT
                     TGTTETASLTQDDLISEGDAICAEANSALGAIESSTADETTKSSQIADIYDGIAQQLG
                     ELGTPSDGEPPTDVIAAAQDLAQGTGDSTTFTTAAGEYGFTDCAEAPESTSYPSTSTG
                     TDSTSTGTDSTGTYTPPATTPEETYTPPTTTAPPATGTGGGVAPTTPTAPSTGGSSGG
                     SSSGGIGPG"
     gene            complement(3075..4922)
                     /locus_tag="PLJ64_02870"
     CDS             complement(3075..4922)
                     /locus_tag="PLJ64_02870"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC92467.1"
                     /translation="MSPALRNRKSRSAGRNREHREIVAGRGRKLHWTFGLLKPYRTRI
                     IFMVMAVIASTAAALAPPYLAGRAIDSGIEAGNIPALNLTIALFVAAIIVNAVTSWLQ
                     TWLVGWVGIRVLQDLREQVFKHIQRMSIGFFNRNRPGALISRMTNDIEALNQLISDGI
                     VTLFANLLTLFGVVAIMLLLDWRLALITFLVLPLLLVTSLVFRKYSAGAFRETREQIA
                     RVTSHLQETLSGVRVVRAFAQEERHISTMTALNEANRQANMKTVYLNASYFPATELLT
                     AVGTSAILAFGGWQAIEGNLMIGVVISFVGYLQLFFDPIQQLAQLYATYQQGMAALDK
                     IFTLLDTEVEIVDSPEAIDPPPLEGRIRFDDLSFTYEDPEVEAESGEPVTAAPAAPRW
                     AVRDIDFEIEPGETVALVGSTGAGKSTLAKLITRFHDPQQGRILIDGRPLADYRQRAL
                     RSQMGIVPQEGFLFSGSIAENIAFGRPDASIEEIAAAADAVGATPFIERLSDGLETEV
                     GERGVQLSSGQRQLVAFARVILAEPRILILDEATSAVDTRTEKVIEKALDEVLEGRTA
                     VVIAHRLSTIRRADRIIVLDHGRIVESGSHTELIAARGRYASLYSSWTA"
     gene            complement(4919..6676)
                     /locus_tag="PLJ64_02875"
     CDS             complement(4919..6676)
                     /locus_tag="PLJ64_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC92468.1"
                     /translation="MPTFRRLLGFLRPHRRELWGSMVFAWLAMGMTVLIPALVGKATN
                     AIDSSDRQQLFWLVGAILLAAVLRLGLTVIRRIVAGRVSLAVEFDLRQEVYAHLMSLE
                     LGFFDRNQTGQLMSRATVDLQSIRFFLGYGLIFFSQSLLTVLLAGVVMFVINPWLALI
                     ALAPTPLIVFTALRYTRQSRPAMQEVAQRVAELTAQAEESVSGIRVVKAFAREDHQFE
                     RFQGAVDRLFDQSIRSTRIQAFFSPLIAALPQLGTALVLLVGGHEVLSGNLDLGQFTA
                     FYVYMLMLAGPLRTVGMALGMGQRAIASGNRLFEVLDREPELFSKPGAGPLPEGGGAV
                     RFDDVSLSYGPEAPDALQGIDLEVPAGTRIALVGPSGAGKTSLAALLARLYDPTGGSV
                     SIDGADLREVDLPSLRHQIAYVSDDSFLFSDTIAENISYARPDATRDEIERAARLAQA
                     SEFIEALPDGYETVVGERGLTLSGGQRQRVAIARALLADPRILILDDATSSLDATTEQ
                     QIRAGLAEVMAGRTTFVIGHRLSTISLADEVLLLEHGRIQDRGTVAELTERSPLFREL
                     TGGSGEEPLFVPEDEEVLA"
     gene            6847..7701
                     /locus_tag="PLJ64_02880"
     CDS             6847..7701
                     /locus_tag="PLJ64_02880"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNC92469.1"
                     /translation="MEKAAVELLICPHCGGELRENGGSLICSRGHTMNIARQGYVSLL
                     GRDSGTHTADSAEMVAARERFLGAGHFQPLVDALVEEASGKPGPAGGESAVVDLGCGT
                     GYYLASVLDALPESIGVGVDNSKFAARRAAKVHPRASAVVADIWDGLPIKDQAAGLIL
                     NVFSPRNGEEIERILAPGGRLVVVTPQPGHLAGLIGRFGMISVDPDKEERLERTLGPL
                     AKAAGSRNLEWEMKLDRAAVRDLIQMGPSAGRMEEAEMESALQALEEVTAVEAAVTVT
                     TTSSEERQ"
     gene            7698..9599
                     /locus_tag="PLJ64_02885"
     CDS             7698..9599
                     /locus_tag="PLJ64_02885"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HNC92470.1"
                     /translation="MSIDRKGASDFTREAHVHDIRCLDFEDNESFEEAKRGFIAPVPD
                     GQILKDDGDFVFDPHRLAFASGDPEQPDTVNPSLWRQARLYADGGLFEVCDRIYQVRN
                     LDISNITFIEGDTGLIVVDPLVSAEVAKAALDLYFEHRGEKEVVAVIHSHSHVDHFGG
                     VRGVVDQADVDSGKVPIIAPEGFLEAAVSENVAAGNVMTRRAMYQFGILLPPDPQGTV
                     GSGLGIGTSLGTITLIPPTDLITETGETRKIDGLTFEFMLTPDTEAPAEMHWYIPELR
                     ALTAAENCTHTLHNTYPIRGAIVRDPQAWSMYLNDTIDRWADKSDVMYAMHHWPVWGK
                     DRVREMLEKGRDAYRYIHDETLRLANHGLTPREIAEQVEFPDSLAKHWSVRSYYGTVS
                     HNVKSTYTRYLGWYDGNPANLEPLVPVESGKRYVEFMGGADNVVSKARESFEKGEYRW
                     VAEVLGHVVFADPANQEARELQADAFEQLGYQTESSTWRNAYLMGAQELRHGTPSFPI
                     GGTASEDSIRAMTLPMIFNFFAIKIHGVDAADLELDVNLDITDTGESAVLRLSNGVLS
                     HSMTDRDEDAPVLEMSRELLNKLVGNEITLEEAVEAGEVESGPGLEVFRKFGEHLDEF
                     DLWFPIIEP"
     gene            9726..10601
                     /locus_tag="PLJ64_02890"
     CDS             9726..10601
                     /locus_tag="PLJ64_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92471.1"
                     /translation="MPETVSGESSANVSAFETQEPTHRVLLVADEVFRGSDLADELAA
                     HGTDRGSIGVFVISPAMTHSLIDQELGNVDQTVIEASDRLETILAELRKVGISARGEV
                     GDADPMVAIGDGVREFEPDQIMVVTHVDSEREYAEKNLWDRLGTDFHAPVTQLKVPHP
                     AGEGGIPAVESVEHFSAHQRTEEEIQTETRNFPPLKKMDIAGILIGFIGTVALGMVAV
                     AVGTEDGGGNLSGSAAIVLLIAMGAFLINVAHIVGLLFFESVRYSGIWQKFMARLSIG
                     ITTIGLAVSLVLWLA"
     gene            10626..11726
                     /locus_tag="PLJ64_02895"
     CDS             10626..11726
                     /locus_tag="PLJ64_02895"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNC92472.1"
                     /translation="MTKRVAVIGAGVVGLAAAWALERRGITCLVFEQGAPGGAQSGGE
                     SRIFRHAHNDPRQFELAVRSRQGWREWEAEFGLDLISPDGSMVIGEAAVARLGKYDGE
                     PGVRIRELGPAQLREVLPILAPYDGPALIDDAAGAIHTRLTIASLITSVGLNLVPERI
                     ESVRPRPEGGVEVVSTFGDRVFDSVVVAAGAGTADLAATAGLKVPSQPQAHMRLTLPL
                     AGGIKAPRLACLQDSSGFFGEAAAYGSPVQGNREYAVGLALSTPVDLDADADPLSGLA
                     DRTADYVAEAMPGLDRGGAVSFQRWVTELPWAADGLAIWQRGEAFFIAGHNLFKLAPA
                     LGSALADCVENGEVGRHFRPEDKLGEAVLEAR"
     gene            complement(11792..12574)
                     /locus_tag="PLJ64_02900"
     CDS             complement(11792..12574)
                     /locus_tag="PLJ64_02900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HNC92473.1"
                     /translation="MDSKVLNRAETTSSIIGLTDEAGLWARRDSDPRASEELVNRYLA
                     FAKSVAIRYRSQSESLDDLVQVASLGLVNAVKRFDPERGVPFIAFASPTITGELKRHF
                     RDRTSAMRLPRSLYDRIGQIDTVVSDLTSQLNREPTVFEIADEMGCPVEEVVEAVEAG
                     HNRQPVSLDQTPGTSEGDQSSPAEWLGFEDSDFETAENRIMLRAAMDELSGEEQMVIQ
                     LRFREELTQSEIAERIGHSQMHVSRMLRRILDRMGDRLPAAA"
     gene            complement(12922..13278)
                     /locus_tag="PLJ64_02905"
     CDS             complement(12922..13278)
                     /locus_tag="PLJ64_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNC92474.1"
                     /translation="MITGLDFIGIPSTDADRSRAFYVDTLGLRPDEGSDYEFWVGETC
                     FGIWEPDKQGMEFSAEKCSHLAFHVEDIEAARAELEKKGVTFFGDTFDTGVCYMAIFA
                     DPDGNSLMLHHRHTPR"
     gene            complement(13319..14071)
                     /locus_tag="PLJ64_02910"
     CDS             complement(13319..14071)
                     /locus_tag="PLJ64_02910"
                     /inference="COORDINATES: protein motif:HMM:NF024934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC92475.1"
                     /translation="MFGEPEGTVRLLDAEPTLARGIERRLLDEAREKLIAEVRRVEAG
                     PWEEARVIPSDPLFLGYLILEGALIREIDVSGRPSIELLGPGDLIRPWVVPQALDALH
                     VNDEWSLLSTGHIAIVDESFHEKAQGYPSILTVLMDRVVARARWLGFQVALSQLTRID
                     HRLLLTFRYVAERWGVDDPRGVKIPIRLSHRNLAALIGARRPKVSSALTTLSECDLIE
                     QSPDGTWTFFAADIDPMAVLAGIDDPPEPIGA"
     gene            complement(14128..17157)
                     /locus_tag="PLJ64_02915"
     CDS             complement(14128..17157)
                     /locus_tag="PLJ64_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92476.1"
                     /translation="MTRRANLPHHPGLIAAASLVALVAACLILPVGARAAGATFVTTG
                     LPMPADGAPGADSGYSDFRPSGSPLSANGRYVAFQSDASFQLKGVRPDETNVFRKDRK
                     TGKVVLVSRRTGPAGAGSRGRTGSVTISGDGNLVGFTTTDQLVPADTDLVADFYIRNV
                     ATNVTTLESPGTDLEVGDGSISADGSYAVFDTTSVVVGVDVNNKPDVFRRRLSDGTTD
                     LVSRIPASATAGDNLSFAGGISGDGRWVTFTSGSSDLVTPFTDTNGGFTYDVFVRDMT
                     DGSTYLVSSVYNDASTTANGGSGEPVVAGSPASAAEVKLSFSSNATDLAPPGTDGATD
                     YSVYTRGLTSPASTLVSLNSSGENANSRAHTPSISNDGKRLVFTSDATNLGGPDTYYG
                     VYLRNLVTNQTSLVSARNEYAVFGSLSADGRVAAWAETGATRDADPFIEGIFARTLPK
                     GKITMASSPAGGKRMVLPGARLNDATIDGPQLSQNGRYEVLTVYTNRLPGYRKGFAQT
                     YRRDLKTGEWTLVSRATGKNGSPAETGSEGASISNDGNRVLFLTRTSLVPIDTDNELS
                     AYIRDLKKATTSLVSRADGASGANADQAVNSAVISGGGNRVAFSTYSTNLGAPGGQEQ
                     VYVRDLAGKKTLLVSRADGEAGAAGNGNSGNPELSDNGQLVVFDSLSTNLHPDDADAN
                     RSIFVRHPGSGSTVLASRRPGLTGANATDTQSSPDISGNGRFVAWRSGDPDTAPEAGP
                     WPIGTSQVVLRNLNSGENSLISRGPGSGPPGELGGASPSLNRNGSVIAFATNSANLIS
                     GLGGADRPAVMVRNLASGGQLSGPPAFGEVGSLASTGSLAPSISANGKCLAFVGLGYS
                     KASGVFNDLRSGYVFAPAGGCPKPPAGGPTISAASMKPAAFPFRSKRGARIRFRLDRK
                     ATVTFLVERKVPGRKVGDECHKVRPWNAGKPGCTKLVYIGRFTRRNLPAGVNVVRFRA
                     RVGKKRLGPGRYRLRLRAADRVGNSRFVPLSFRLTRR"
     gene            complement(17167..17838)
                     /locus_tag="PLJ64_02920"
     CDS             complement(17167..17838)
                     /locus_tag="PLJ64_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92477.1"
                     /translation="MHRHLTPSLVLSVIAVVIALGGTSYAISQLPKNSVGARQLKKNA
                     VTAAKLKKDSVSSAKVKDGSLLKKDFKQGQIPKGQTGAAGATGASGPTGPSGPTGETG
                     ETGAPGSAVAYAYVQDIGDPNQSIPSDTAKNMQSSMFSKSQTNPTGVYCFDLESIWPI
                     KNFSVVAESNYNNLPESDKFATGQIQHNNDFGVGCPNTTDLLVITRDASTGNLVNWFF
                     YLTLN"
BASE COUNT         3136 a         5675 c         6072 g         3016 t
ORIGIN      
        1 gtcttcgggc tggcggtgat cggtgacgtt gaagccctgg cggggatcta cgcctttggc
       61 gccaccctgg cgatcaccat cgccaacctt tccgttcttc ggttgaggcg gtcgatgccg
      121 gacgccgagc gtcccttcag ggcgcccttc aacctgaaga tcggctcggt cgaccggccc
      181 ctcagcgccg tgatcggcgc ggttctcagc agcctcgccc tggtctcggt tctgctcttc
      241 cacgattccg cccgctgggt cggactcggc tggctggcgg tcgggctggt cggctatgtc
      301 atctatcgac gggtggtcga gcagatcagc ctgaccaagc aggtagcggt tgaaccgcgg
      361 gccctgaccc ggccccgggt gtcaatcgac tttcacaaca tcctcgtgcc gatcttcggc
      421 acccgcatcg atgacgacat cgtctcgaca gccggccgga tggcagccga aggtgacccg
      481 gaggaggagc acaccaaggc ggtcatgaca atcctctatc tgatcgagat tcccctgacg
      541 cgggcgatca gggatcccct gccggaggag gtggccgagg aagcccagcg cgcgaccaaa
      601 cgggccaagg aggtcgccga cgagtacgag ggggtcgaga tcagggtcga gatcgagcgg
      661 gtccggcgca agggaaccgg catcatcttt gcggcccggc gactcgacgt cgatgcgatc
      721 gtgatgggcg ccgagccgcc gagcccgatc aagggcggcg cccaactggg cgggatcggc
      781 gactaccggc ccgaggaaat cggaccgatc accgcctacg tcctcaaacg ggccccctgc
      841 cgcgttctgt tgaccgcgcc cagaaccgcc tgagcgtagg atgcccccat gttcgtcttg
      901 atcgttggat gcgggagggt cggttccacc gtcgcccgct cgatgctgtc caaggggcat
      961 tcggtttcct gcctggacga ggaccccgaa tcgcacgccc ggctggaagt cggcctcgat
     1021 acctcatggg aggaagcggg cggccagttc acggttggca ccgcgctgga aacccaggcc
     1081 ctcgaagggg cggggatcat cgaggccgac gccttcgtgg ccgctacaaa tggcgacaac
     1141 acgaacatcg tgatcgccca gatcgcacag aagcgttatg aggttgaaac ggtgatcgcc
     1201 cggatcctcg atccgctgcg ggccgagtgg taccgcgagc aggggctgcg gacgatctgc
     1261 ccgaccaagg tggcggtcga gatgctcgag gacaagctgc tgagcgcggc gaacccgacc
     1321 tcgctcgacg ccggcagcgc cgaattcgcc gattcgccgc cggcgccgcc cgcggggccc
     1381 gcgaccggag gggacgagta gatgtacgtg atcgtcgccg gggccggcaa ggtcggtgtg
     1441 aacctgaccc gggagctgct cggccagggc catgaagtca cgctgatcga aaaccgtcgc
     1501 cagcgttacc tgggcgtcga gcagctgctc gagcacaaca tcctttacgg cgatgcctcc
     1561 gagctctggg tgcttgaaag ggccggtatc gagcgcgccg agatggtgat cgcggtcacc
     1621 ggcgatgacg aggacaacat cctgatctgc caggtcgcca aggagaagta cggaatcggc
     1681 cggatcatcg cccgtgtgaa caaccccagc aaccgggccc atttcgacct gctcgggatc
     1741 aagcctgtgg tttcggcgac cgaactgatc ctgcgcctgc tcgagcacga ggtgccgcac
     1801 cacaacctgg tccatctgct tgacctcgag gaagaggaga tcgagatcat cgagatccgc
     1861 ctcggcagca agtcgccggt ggtcggccgc tcggtcggcg aggtgggcac gccggacaac
     1921 tcactggtca tcgcgatcat tcgcgaaggc cgcggggtgg ttcccaacgc ctcgacggtc
     1981 ttcgaagaag gcgatgacat cctcgtcgtg ctcgacccgg agcgagaaga agagctgaag
     2041 cagatcttca ccgacggctg agggcgggca tgaaaaagcc cggcgctgag gccgggctga
     2101 aaggctgcgg gacagggact cgaaccccga tttcacggac cagaaccgcg cgaattacca
     2161 ttattctatc ccgcagcggt ggtcctcaag gatatagacg cggccccgca agtgcagtct
     2221 gaaaagggag ggccggccgt tttgccccgc aatcgggcac ttttgagcct gaccgcgcct
     2281 tctgttacct tgcgtacgtg cccaccacag gatttgactc tcgccgcgtt cttggccttg
     2341 ccctgatctc gcttgtcatc tcggccggcg ccatcggctg cggcggcgga ggcgacgacg
     2401 agacggcggc cccggtgccg accaccacca ccgggacgac cgaaaccgct tccctgaccc
     2461 aggacgacct gatctcggag ggcgacgcga tctgtgcgga agccaattcc gcgctcggag
     2521 cgatcgagtc atcgaccgcg gacgagacga ccaagtcttc ccagatcgcc gacatctacg
     2581 acggcatcgc ccagcagctc ggggagctcg gcacccccag cgacggtgaa ccgcccaccg
     2641 acgtgatcgc ggctgcccag gaccttgccc agggcaccgg ggattcgacc accttcacca
     2701 ccgccgccgg cgagtacggc ttcaccgact gtgccgaggc ccccgaatca acctcgtacc
     2761 cgtccacttc aaccggtacc gacagcactt cgaccggcac cgattcaacc ggcacctaca
     2821 cccctccggc caccactccg gaagagacct ataccccgcc gaccaccacc gcaccgcccg
     2881 ccaccggcac cggcggcggg gtcgccccga ccacgccgac tgcccccagc accggtggca
     2941 gctccggcgg cagcagcagc ggcggcatcg gcccgggctg acgggcccga ccgcgccgcc
     3001 gcccggctac acccgattca tcgagatccc cgtttgtgcc acataccggc acaaagtggg
     3061 cactcgacgc aagatcaggc agtccagctc gagtagagcg acgcgtagcg gcccctggcc
     3121 gcgatcagtt ccgtgtgact tcccgattcg acgatccggc catgatcaag gacgatgatc
     3181 cggtcggccc ggcggatcgt cgagaggcgg tgggcgatca ccacggcggt gcgcccttcg
     3241 aggacctcgt cgagggcttt ctcgatcacc ttctcggtgc gggtatcgac cgctgaagtg
     3301 gcttcgtcga ggatcaggat ccgcggctcg gcgaggatca cccgggcgaa ggcgaccagc
     3361 tggcgctgcc ctgacgaaag ctggactcca cgctcgccga cttcggtctc gaggccgtcg
     3421 ctcaggcgct cgatgaacgg ggtggccccg accgcgtcgg ccgccgcggc aatctcctcg
     3481 atcgaggcgt cgggccggcc gaaggcgatg ttctcggcga tcgagccgga gaacaggaag
     3541 ccttcctgcg gcacgatccc catctggctt cgcagggcgc gctgccggta gtcggcgagc
     3601 ggccggccgt cgatcagaat ccgaccctgc tgcggatcgt ggaatctcgt gatcagcttg
     3661 gccaaagtcg acttgccggc accggtgctg ccgaccaggg caaccgtctc acccggttcg
     3721 atctcgaagt cgatgtcgcg gaccgcccag cgcggtgcgg ccggtgcggc cgtgaccggt
     3781 tcgcccgatt ccgcctccac ctcgggatcc tcgtaggtga acgagagatc gtcgaagcgg
     3841 atccggcctt cgagcggggg cgggtcgatc gcctcggggg aatcgacgat ctcgacctcg
     3901 gtgtcgagca gggtgaagat cttgtcgagg gcggccatgc cctgctgata ggtggcgtag
     3961 agctgggcga gctgctggat cgggtcgaag aagagctgca ggtaaccgac aaaggagatg
     4021 accacgccga tcatcaggtt gccctcgatc gcctgccagc cgccgaaggc gaggattgcc
     4081 gaggtgccga ccgcggtcag gagctcggtc gccgggaagt agctcgcgtt gagatagacc
     4141 gtcttcatgt tggcctggcg gttggcttcg ttcagggcgg tcatcgtgct gatgtgacgc
     4201 tcttcctggg cgaaggcgcg gaccacccgg accccgctca gggtttcctg caggtgcgag
     4261 gtgacccggg cgatctgctc gcgggtctcg cggaaggcgc cggccgagta cttgcggaac
     4321 accaggctgg tgaccagcag gagcggcagg accaggaagg tgatcagggc cagccgccag
     4381 tccagcagca gcatgatcgc gaccaccccg aacaaagtga gcaggttcgc aaacagggtc
     4441 acgatgccgt cgcttatcag ctggttcagc gcctcgatgt cattggtcat gcgggaaatc
     4501 agggcaccgg gccggttccg gttgaagaat ccgatcgaca tgcgctggat gtgtttgaag
     4561 acctgctcgc ggagatcctg caggacccgg atcccgaccc agccgaccag ccaggtctgc
     4621 agccaggaag tgactgcgtt gacgatgatc gcggcgacga agagggcgat cgtcaggttc
     4681 agcgccggga tgttgcccgc ctcgatgccg gagtcgatag cccggcccgc caggtacggc
     4741 ggtgccagag cggcggcggt cgaggcgatc acggccatga ccatgaagat gatccgggtc
     4801 cggtacggct tcagcaggcc gaaagtccag tggagcttgc ggccccggcc ggcgacgatt
     4861 tcccgatgct cgcggttccg accggcgctt cgcgatttcc ggtttcgaag ggccgggctc
     4921 atgccagcac ctcctcgtct tcgggaacga agagcggctc ctcgcccgag ccgccggtga
     4981 gttcgcggaa gagcggcgag cgttcggtca actccgcaac cgtgccgcgg tcctgaatcc
     5041 ggccgtgctc cagcagcagg acctcgtcgg ccagggaaat ggtggagagg cggtggccga
     5101 tcacaaaggt ggtgcggccg gccatgacct cggccagccc ggcgcgaatc tgctgctcgg
     5161 tggtggcgtc aagcgaggag gttgcgtcat cgaggatcag gatgcgcggg tcggccagca
     5221 gcgcccgggc gatcgccacc cgctggcgct ggccgccgga aagggtgaga ccgcgttcac
     5281 cgaccaccgt ctcgtagcca tccggcagag cctcgatgaa ctcgctcgcc tgggcgagcc
     5341 gggctgcccg ctcaatctcg tcccgcgtcg cgtcaggccg ggcgtaggag atgttctcgg
     5401 cgatcgtgtc gctgaacagg aaactgtcat cggagacgta ggcgatctgg tggcgaagcg
     5461 acgggaggtc aacctcgcgg aggtcggcgc cgtcaatcga aacgctgccg ccggtcgggt
     5521 catagagccg ggcgagcagt gcggcgaggc tggtcttgcc ggcaccggaa gggccgacca
     5581 gggcgatccg ggtcccggcc ggaacctcaa ggtcgattcc ctggagggca tccggcgcct
     5641 ccgggccgta gctgagcgag acatcgtcaa accgcaccgc accgccaccc tcgggcaagg
     5701 gcccggcgcc cggctttgaa aaaagctccg gttcgcggtc cagcacctcg aagaggcggt
     5761 ttccggaagc gatcgctcgc tggcccatcc ccagtgccat gccgaccgtg cgaagcgggc
     5821 cggccagcat cagcatgtag acgtagaagg cggtgaactg gccgaggtca aggttgccgc
     5881 tcaggacctc gtggccgccg accagcagaa ccagggcagt gccgagctgg ggcagggcgg
     5941 cgatcagcgg cgagaagaag gcctggatcc gggtcgagcg aatcgactgg tcaaacaggc
     6001 ggtcaaccgc gccctggaag cgctcgaact ggtgatcctc gcgagcgaac gccttgacca
     6061 cgcggattcc ggaaacgctc tcctcggcct gggcggtgag ctcggcgacc cgctgggcca
     6121 cctcctgcat cgccgggcgc gactgacggg tgtagcggag cgcggtgaac acgatcagcg
     6181 gagtgggggc cagggcgatc agcgcgagcc agggattgat cacgaacatg accacgcccg
     6241 ccagcaacac ggtaagcagg ctctggctga agaagatcag gccgtaaccg aggaagaagc
     6301 ggatcgattg caggtcgacc gtggcccggc tcatcagctg gccggtctgg ttgcggtcga
     6361 agaaaccaag ctcgaggctc atcaggtggg cgtagacctc ctggcgaaga tcgaactcga
     6421 ccgccagcga gaccctgccg gcgacgatcc gtctgatcac cgtgagcccg agccgcagga
     6481 ccgcggcaag gaggatcgcc ccgaccagcc agaagagctg ctgccggtca ctgctgtcga
     6541 tcgcgttggt ggccttgccg accagggccg ggatcagcac cgtcatgccc atcgccagcc
     6601 aggcaaagac catggaaccc cagagctcgc ggcgatgggg cctcaggaat cccagaagtc
     6661 gcctgaaggt gggcatcccc ttcactatac aaaatgaagt aatggggttg gtcagggaca
     6721 ttggggcggc gaatgcccac tttgtgccgg tatgtggcac aaagtgggca ttcgcgaagt
     6781 cgggtggatc ccggttcgga gttcgtcagg agcggtgggg ctatcgagcc aaatagagtg
     6841 cgcggggtgg agaaggccgc ggttgaactg ctgatctgcc ctcattgcgg gggcgagctt
     6901 cgcgagaacg ggggctcgct gatctgttcc cgcggccaca cgatgaacat cgcccggcag
     6961 ggctacgtga gcctgctggg ccgggactcc ggcacacata ccgccgattc ggccgagatg
     7021 gtcgccgcca gagaacgctt cctcggtgcc ggccacttcc agccgctggt cgatgccctg
     7081 gtcgaggaag catcagggaa gcctggaccg gctgggggcg agtcggcggt ggtcgacctc
     7141 ggatgcggga ccggctacta cctggcctcg gttctggacg ccttgccgga aagcatcggg
     7201 gtcggggtcg acaactccaa gtttgccgcc cgccgggccg cgaaagtcca cccgcgggcc
     7261 agcgccgtcg tggccgacat ctgggacggc ctgccgatca aagaccaggc ggcgggcctg
     7321 atcctgaacg tcttttcgcc ccgcaacggg gaggagatcg agcggattct cgcccccggc
     7381 ggccggctgg tcgtggtcac accgcaaccc ggacacctgg ccgggctgat cgggaggttc
     7441 ggaatgatct cggtcgaccc ggacaaggag gagcggctcg agcggacgct cgggcctctc
     7501 gccaaggcag ccgggagccg gaatctcgaa tgggaaatga agctggaccg ggcggcagtc
     7561 agggatctga tccagatggg gcccagtgct ggaagaatgg aggaagccga aatggaatcg
     7621 gcgctccagg cactggagga ggtgaccgcg gtggaggccg cggtgaccgt caccacaacg
     7681 agcagcgagg agaggcaatg agcatcgatc gcaagggcgc atccgacttc acccgggagg
     7741 cccacgttca cgacatccgc tgtctggact tcgaagacaa cgagtcgttc gaggaagcaa
     7801 aacgcgggtt catcgccccg gtcccggacg gccagatcct gaaagacgac ggtgacttcg
     7861 tattcgaccc gcaccggctc gccttcgcct ccggggaccc ggaacagccg gacaccgtga
     7921 accccagcct ctggcgccag gcccgccttt acgccgacgg cggcctcttc gaggtctgcg
     7981 accggatcta ccaggtgcgg aacctggaca tctccaacat caccttcatc gaaggcgaca
     8041 ccgggctgat cgtggtcgac ccgctggttt cggccgaagt ggcgaaggct gcgctggatc
     8101 tttacttcga gcaccggggc gagaaggagg tcgtcgcggt gatccactcg cacagccacg
     8161 tcgaccattt cggcggcgtg cggggggtgg ttgaccaggc ggacgtggat tcgggcaagg
     8221 tcccgatcat cgcgccggaa gggttcctcg aagcggcggt ctccgagaac gtggcggccg
     8281 gcaacgtaat gacccggcgg gcgatgtacc agttcggcat cctgctgccg ccggaccccc
     8341 agggcacggt cggcagcggc ctcggcatcg gtacttcgct gggcacgatc accctgatcc
     8401 ccccgaccga cctgatcacc gagaccggcg agacccgcaa gatcgacggg ctgaccttcg
     8461 agttcatgct gaccccggac accgaggccc cggccgaaat gcattggtac atcccggagc
     8521 tgagggcgct taccgcggcc gaaaactgca cccacaccct gcacaacacc tacccgatcc
     8581 gcggggcgat cgtgcgcgac ccgcaggcct ggtcgatgta cctgaacgac acgatagatc
     8641 gctgggccga caagtcggat gtgatgtacg cgatgcatca ctggccggtc tggggcaagg
     8701 accgcgttcg ggagatgctc gaaaagggcc gggacgcata tcgatacatc cacgatgaaa
     8761 ccctgcgtct cgccaaccac ggtctgaccc cccgtgagat cgccgagcag gtcgagttcc
     8821 cggattcgct ggccaaacac tggtcggtcc gcagctatta cggcacggtc agccacaacg
     8881 tcaagtcgac ctacacccgg tacctcggct ggtatgacgg caacccggcc aacctcgagc
     8941 cgctggttcc ggtggaatcc ggaaagcgct acgtcgagtt catgggcggg gccgacaatg
     9001 tggtcagcaa ggcccgggaa tcattcgaaa agggcgagta ccgctgggtc gcggaagtgc
     9061 tcggacacgt cgtcttcgcc gatcctgcca atcaggaggc ccgcgaactg caggcggacg
     9121 ccttcgagca gcttggctac cagaccgaat catcgacctg gcgaaacgcc tatctgatgg
     9181 gtgcccagga actccggcac ggcaccccga gcttcccgat cggtggcacg gccagcgagg
     9241 actcgatccg ggcgatgacg ctgccgatga tcttcaactt cttcgcgatc aagattcacg
     9301 gcgtcgacgc ggccgacctc gaactggacg tgaacctgga catcaccgac accggggaat
     9361 ccgcggtgct gaggctctcg aacggcgtgc tcagccattc gatgaccgac cgggatgagg
     9421 atgcgccggt gctcgagatg tcccgggagc tcctcaacaa gctggtcgga aacgaaatca
     9481 cgctcgagga agcggtcgaa gcgggcgaag tcgagtcggg tcccggtctc gaggtcttcc
     9541 gcaagttcgg cgagcatctg gacgagttcg acctctggtt cccgatcatc gagccgtaaa
     9601 cggaatcgaa ggcgagacga aaaatcccgc cttcgccgct gctttcccga ggccgctcag
     9661 cccggtgctc cgggaaattc cgtaaaaagc ggttccggtt gcctgaagcc gggtaggttg
     9721 aaaatatgcc tgagaccgtt agtggtgaat catccgcgaa cgtttcggcg ttcgaaaccc
     9781 aggaaccaac tcaccgggtg ctgctggttg ccgatgaggt cttccgcgga agtgacctgg
     9841 ccgacgagct ggcagcccac ggaaccgatc ggggctcgat cggggtcttc gttatctcac
     9901 ccgcgatgac ccattccctg atcgaccagg aactcggaaa cgtcgaccag acggtgatcg
     9961 aagcgtcgga ccgccttgag acgatcctgg ccgaactgcg caaggtcggc atttcggccc
    10021 gtggcgaggt cggtgacgcc gacccgatgg tggcgatcgg agacggagta cgggaattcg
    10081 agcccgacca gatcatggtg gtcacccacg tcgacagtga gcgggagtat gccgagaaga
    10141 atctctggga ccggctcggg accgactttc atgctccggt cacccagctg aaggttcccc
    10201 acccggctgg cgagggcggg atccccgcgg tcgagtcggt tgagcatttc tcggcccatc
    10261 agagaaccga agaagagatc cagaccgaaa cccgcaactt cccgccgctg aagaagatgg
    10321 acatcgccgg gatcctgatc gggttcatcg ggaccgtcgc cctcgggatg gtggcggtcg
    10381 ccgtcggcac cgaggacggc ggtggcaacc tctcgggttc cgcggcaatc gtgctgctga
    10441 ttgcgatggg tgcgttcctc ataaacgtgg ctcacatcgt cggactgctg ttcttcgaaa
    10501 gcgtcagata tagcggcatc tggcagaaat tcatggccag gctctcaatc gggatcacga
    10561 cgattggcct tgcagtctca ctcgttctgt ggctcgcctg accccgatca agcaaaatca
    10621 gggccatgac gaagcgggtc gcggtaatcg gagccggggt ggttggtctg gccgccgcct
    10681 gggcgctgga gcgccggggc atcacctgcc tcgtcttcga gcagggtgct ccgggcgggg
    10741 cccagtccgg tggtgaatcg cggatcttcc gccatgccca caacgacccg aggcagttcg
    10801 agctcgcggt cagatcgcgc cagggctggc gtgaatggga ggccgaattc ggcctcgatc
    10861 tgatctcgcc cgacggatcc atggtgatcg gcgaggcggc ggtcgcccgg cttggcaagt
    10921 acgacgggga gcccggcgtg cggatccgtg aactcgggcc ggctcagctc cgggaagtgc
    10981 tgccgatcct cgctccttac gacggaccgg ccctgatcga cgatgcggcc ggggcgatcc
    11041 acacccggct gacgatcgcc agcctgatca cctcggtcgg gctcaacctg gtcccggagc
    11101 ggatcgaatc ggtccggccc cgcccggaag gcggggtcga ggtcgtgagc accttcggcg
    11161 accgggtctt tgactccgtc gtggttgctg ccggcgctgg tacggccgac ctggctgcga
    11221 cggccggcct gaaggttcct tcccagccgc aggcccacat gcgcttgacc ctgccgctcg
    11281 cgggcggaat caaggctccc cgcctggcct gccttcagga ctcttcgggg ttcttcggcg
    11341 aggcggccgc ctacggatca ccggttcagg gaaaccgtga atacgccgta gggctggccc
    11401 tcagcacccc ggtcgacctg gacgctgacg ctgaccccct ctccgggctg gccgatcgca
    11461 ccgcggacta cgtggcggaa gccatgccgg gcctcgaccg cggcggggcc gtttcatttc
    11521 agcgatgggt gaccgagctg ccctgggcag cggacggtct cgcgatctgg cagcggggcg
    11581 aggcgttctt catcgctggc cacaatctgt tcaagctcgc tcccgccctg ggctccgccc
    11641 ttgccgattg tgtggaaaac ggcgaggtgg ggcggcactt ccggccggaa gacaaactcg
    11701 gcgaagcggt tctcgaagct cgctgattaa acaaaccgac cggccagggg gcgggccggt
    11761 cggagtgttt cctttggggg agtcgggagg gtcaggctgc ggccggaagc cgatcgccca
    11821 ttcggtcgag gatccgccgc agcatccggg acacatgcat ctgggagtgg ccgattcgct
    11881 cggcgatctc ggactgggtc agctcctccc ggaagcgaag ctgaatcacc atctgctctt
    11941 cgccgctcag ttcgtccatc gcggcccgga gcatgatgcg gttctcggcc gtctcgaaat
    12001 ccgaatcttc gaagccaagc cattccgccg gtgaactctg atcgccctcc gaggtgccgg
    12061 gggtctggtc gagagaaacc ggttggcggt tgtggccagc ctcaacggct tcgaccactt
    12121 cctcgacggg gcatcccatc tcgtcagcga tctcgaagac tgtgggttcg cggttgagct
    12181 ggctggtcag gtctgaaacg acagtgtcga tctggccgat ccggtcatag aggctgcggg
    12241 gcaggcgcat cgccgaggtc cggtcgcgaa agtgacgctt gagctcgccg gtgatggtcg
    12301 gggaggcgaa ggcgatgaac ggcaccccgc gttccgggtc aaaccgcttc acggcgttga
    12361 ccaggccgag gctggccacc tggaccaggt cgtccaggga ttcggactgg ctgcggtacc
    12421 tgatcgccac gctcttggcg aaggccaggt aacggttgac cagttcctcc gatgcgcggg
    12481 ggtcgctgtc acggcgggcc cagagtccgg cctcgtcggt caatccgatg atgctgcttg
    12541 ttgtttccgc cctgtttaga accttgctgt ccatcgctca gcctctcttt ctggaggagt
    12601 gcccctccgg agttgctgcc ctggaaccct ctttttcggg gccgtgcagt ggtgcctgat
    12661 cgttcgggga aaccgtaagc agcgaccggt gaactgtcca gtatccgaaa cctaaagaaa
    12721 gccttaagtt tccgaacggc cttcagaaag tggcttcaga attgggtttc gagcccctgt
    12781 ttttggcgca acaagtggag aacagcctgt ttcacaaaaa agacaacaaa gtacgcaagt
    12841 gttgcgccca gggcgcggaa ttgatcgaaa atgccgcctc gaccggccaa agaagcgcgc
    12901 ggcgcagggt tcgtgcccgg ctcagcgagg ggtgtgccgg tggtggagca tcaacgaatt
    12961 gccgtcggga tcggcgaaga tcgccatgta acagaccccg gtatcgaagg tgtctccgaa
    13021 gaaggtcacg cccttctttt caagttcggc cctggccgcc tcgatgtctt cgacgtgaaa
    13081 ggccagatgg gagcatttct cggccgagaa ctccatgccc tgcttgtccg gttcccagat
    13141 cccgaagcag gtttcgccca cccagaattc gtagtcggag ccttcgtcgg ggcgaagtcc
    13201 gagcgtgtcc acgtaaaagg cgcgcgaacg gtcagcgtcg gtggaaggaa tgccgatgaa
    13261 atcgagtccg gtgatcatgg accaaaggta tccggtcacc ggtccggccg aaactgcttc
    13321 aggcgccgat cggctccggg gggtcgtcga ttccggccag aaccgccatc gggtcgatgt
    13381 cggcggcaaa gaaggtccag gtaccgtccg ggctctgctc gatcaggtcg cattcgctca
    13441 gggtggtgag agccgagctg accttcggcc gcctggcgcc gatcagtgcg gccaggtttc
    13501 ggtggctcag ccggattgga atcttcacgc cgcgcgggtc atcgacaccc cagcgttcgg
    13561 ccacatagcg gaaggtgagc agcagccggt gatcgattcg tgtcagctgg ctcagcgcaa
    13621 cctggaaccc gagccagcgc gcgcgggcga ctacccgatc catcagcacg gtgaggatgc
    13681 tcgggtatcc ctgggccttt tcgtgaaagc tctcgtcgac gatcgcgatg tgaccggtgg
    13741 aaagcagcga ccactcgtca ttcacatgca gggcatcgag tgcctggggc acgacccagg
    13801 gccggatcag gtcgcccggg ccgagcagtt cgatcgaagg ccggccggaa acgtcgatct
    13861 cgcggatcag ggctccctcg aggatcaggt agccgaggaa gagggggtcg gaggggatca
    13921 ctctcgcttc ttcccagggg ccggcttcga cccgccggac ttcggcgatc agcttttcgc
    13981 gcgcctcatc cagcaaccgt cgctcgatgc cgcgggcgag ggtcggttcg gcatccagca
    14041 gccttaccgt gccttccggt tccccgaaca tgtcctgacc ctaaaccccg gggtggaagt
    14101 gaagggcccc cgggcccggc ggggccccta ccttcgcgtc agccggaagg aaagcggcac
    14161 gaacctcgag ttgccgaccc ggtcggcggc gcgcagccgc agccggtacc ggccggggcc
    14221 cagccgcttc tttccgaccc gggccctgaa ccgcaccacg ttgacgcccg cgggcaggtt
    14281 ccgtcgggtg aaccgtccga tgtagaccag cttggtgcac ccgggcttgc cggcattcca
    14341 gggtcgaacc ttgtggcact cgtcgccgac cttgcggccc gggacctttc gttccaccag
    14401 gaacgtgacg gtcgccttgc gatcgaggcg gaagcggatc cgcgcacccc gcttcgagcg
    14461 gaaggggaag gcggccggtt tcatcgaggc ggccgagatc gtcggtccgc cggcgggggg
    14521 cttcgggcag ccgcctgcgg gggcgaacac atatccgctt cgcaggtcgt tgaaaacgcc
    14581 gctcgccttg ctgtagccca gaccgacgaa agccagacac tttccgttgg cgctgatcga
    14641 aggggcaagc gagccggtcg aagccagact cccgacttcc ccgaaagccg gcggcccgct
    14701 gagctggccg ccactggcga gattgcggac catgaccgcc ggccggtcgg cgccgcccag
    14761 cccgctgatc aggttggctg aattggtggc gaaggcgatc accgaaccgt tccggttgag
    14821 actcggcgac gccccgccga gttcaccggg cggcccgctg ccgggaccgc ggctgatcag
    14881 gctgttctct ccgctgttga ggtttctcag cacgacctgg ctggtgccta tgggccacgg
    14941 gccggcctcc ggggcagtat ccgggtcgcc ggatctccag gccacgaacc gcccgttgcc
    15001 gctgatgtcg gggctggact gggtgtcggt ggcgttggca ccggtgaggc cggggcggcg
    15061 cgaggccagc acggtcgagc ccgaaccggg gtggcgaacg aaaatgctcc ggttggcgtc
    15121 ggcgtcatcg ggatggaggt tggtcgaaag gctgtcgaag acgaccagct gaccgttgtc
    15181 gctcagctcg ggattgcccg agttcccgtt gccggccgcc ccggcctcgc cgtcggcgcg
    15241 ggaaaccagc agcgtcttct tgccggcaag gtccctcaca tagacctgct cctggccgcc
    15301 cggcgccccg aggttggtcg agtaggtgga gaaagcgacc cggttcccgc caccgctgat
    15361 cacggccgag ttgaccgcct ggtccgcgtt ggcgccgctt gctccgtcgg ccctcgatac
    15421 caggctcgtc gtcgccttct tcagatccct gatgtaggcg ctgagctcgt tgtcggtgtc
    15481 gatcggaacg agagatgtgc gagtcaggaa aagtacccgg ttgccgtcat tgctgattga
    15541 ggcgccttcg gagccggttt ccgcgggtga accgttcttc ccggtggccc ggctgaccag
    15601 ggtccactca ccggtcttca gatcccgccg gtacgtctgg gcgaagccct tccggtaacc
    15661 aggcagacgg ttcgtgtaga cggtaagtac ctcgtagcgc ccgttctggc tgagttgcgg
    15721 accgtcaatg gttgcgtcgt tgagcctcgc gccgggcagg accattcgct tgccgccagc
    15781 cgggctggac gccatcgtga tcttcccctt cggcagggtg cgagcgaaaa ttccttcgat
    15841 gaacgggtcc gcatccctgg tcgccccagt ctcggcccat gcggctacac ggccgtcggc
    15901 gctgaggctc ccgaagaccg cgtactcgtt gcgagcggaa accagactgg tctggttggt
    15961 gaccaggttt cgcagataga cgccgtagta ggtgtcgggg ccgccgagat tggtcgcgtc
    16021 ggaggtgaag acaaggcgtt tgccatcgtt gctgatcgaa ggggtgtggg cccggctgtt
    16081 cgcgttctcg ccgctggaat tgaggctgac cagggttgac gcgggggagg tgaggccgcg
    16141 ggtgtagacg gagtagtccg tggcgccgtc ggtgccgggc ggcgcgaggt cggtcgcgtt
    16201 cgaggagaag gagagcttga cctcggcggc gctggccgga ctgcccgcga ccaccggctc
    16261 tcccgagccg ccgttggcgg tggtgctggc gtcgttgtag acggaactga ccaggtaggt
    16321 gctgccatcg gtcatgtcgc ggacgaagac gtcgtaggtg aagcccccgt ttgtatcggt
    16381 gaagggggtg accagatcgg acgagccgct cgtgaaggtg acccagcggc cgtcccccga
    16441 gatgccgccg gcgaaggaga ggttgtcacc ggccgtggcg gaagccggaa tcctcgaaac
    16501 gaggtcggtg gtgccgtccg aaagcctgcg gcgaaagacg tcgggcttgt tgttcacatc
    16561 gactccgacc acaacggagg tggtgtcgaa caccgcatag ctgccgtcgg ccgagatcga
    16621 gccgtcgccg acttcgaggt cggtgcccgg cgactcgagt gtggtcacgt ttgtggctac
    16681 gttccggatg tagaagtcgg cgacgaggtc ggtgtccgcg ggaacgagct ggtcggtcgt
    16741 ggtgaagccg accagatttc catccccgct aatcgtcacg ctgccggtgc gtcccctgct
    16801 gcccgcccct gccggaccgg ttcgacggct gaccagcacg accttgccgg tcttccggtc
    16861 cttgcgaaac acgttcgttt cgtccggccg gactcccttg agctggaagc ttgcgtcgga
    16921 ctggaaggcc acgtaacggc cgttcgcact gagtgggctg ccggaagggc ggaagtcgct
    16981 gtagccggaa tcggccccgg gtgcaccgtc ggcaggcatc ggaagcccgg tcgtgacgaa
    17041 ggtcgcgccg gcggcccggg cgccgaccgg aagaatcagg caggcagcga ccagcgcgac
    17101 gagtgacgcc gcggcgatca gacccggatg gtgcggaagg tttgctctcc ttgtcatgac
    17161 cggcccctag ttgagggtga ggtagaagaa ccagttgacg aggttgcccg tgctcgcatc
    17221 ccgggtgatg accaacaggt cggtcgtgtt cgggcagccg accccgaagt cgttgttgtg
    17281 ctggatctgg ccggtcgcga acttgtcgct ttcgggcagg ttgttgtagt tcgattcggc
    17341 cacgaccgag aagttcttga tcggccagat cgactcgagg tcgaagcaat agaccccggt
    17401 cgggtttgtc tgcgacttgg aaaacatcga gctctgcatg ttctttgcag tgtcggaggg
    17461 gatggactgg ttgggatcac cgatgtcctg gacgtaggcg taggccacgg ccgaaccggg
    17521 ggctccggtc tccccggtct cgccggtcgg cccgctgggt ccggtcggcc cgctggcccc
    17581 ggtcgcaccg gcggcaccgg tctgtccctt cggaatctgg ccctgcttga agtccttctt
    17641 cagcagggag ccgtccttga ccttggccga actgaccgag tccttcttga gcttggcggc
    17701 ggtcaccgcg ttcttcttca gttgtcgggc cccgaccgaa ttcttcggta gctggctgat
    17761 cgcgtagctg gttcctccca gggcgatcac cacggcgatt actgaaagga cgagtgacgg
    17821 tgtcaggtgc ctgtgcatga ccccaatcta cggccgccgg cggcggaggg caagggattc
    17881 cagccgcccc gtcggccgg
//
LOCUS       DAOQKN010000024        17430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_1469_length_17430_cov_5.801381, whole genome shotgun sequence.
ACCESSION   DAOQKN010000024 DAOQKN010000000
VERSION     DAOQKN010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 17430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17430
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1469_length_17430_cov_5.801381"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..944)
                     /locus_tag="PLJ64_02925"
     CDS             complement(<1..944)
                     /locus_tag="PLJ64_02925"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNC92478.1"
                     /translation="MTEPTILADRYELRDKLGSGGMSNVYRATDRILERTVAVKVLAE
                     HLSDDDRFVARFRREALAVAKLIHPNIVQVYDTGVDHGRHFIVMECVEGKSGAQILKT
                     EGLVDPEVTVEIGVQACAGLEYAHRHGIIHRDVKPGNLMVIGGPVGGGEMTCKLTDFG
                     IARAAEQTRITQVGSVVGTAAYLAPEQVRGEEATPSTDVYALGVVLYQFLTGRLPYEG
                     SSLAELAVRQQNEKPLPPRTYNADVPETVSAAVMRALEGDKAYRFNTAAALSDGLQRG
                     LRGEDVTLQMDEEPVTPAEFPQAETATRPLERTARTAHR"
     gene            complement(951..1580)
                     /locus_tag="PLJ64_02930"
     CDS             complement(951..1580)
                     /locus_tag="PLJ64_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92479.1"
                     /translation="MPFFARHRPRRGFLPALFASLALSLLLAGCGEDRSNLIPEETSN
                     SLISKINRAEALAADHKCFAAGDVAASAQAEIEGLGGKIDPELKRSLIDGVTSLQTML
                     SDQDICIDEGTVTEEPIQTEVPETDTGTGGTTGETGTTDTQTTTTGTQGTTTDENQNT
                     DQTQNGDNGQQTEPTPPVTNPTSPTTPTNPTTPTTPPSTGPGSGGLGPG"
     gene            complement(1635..2372)
                     /locus_tag="PLJ64_02935"
     CDS             complement(1635..2372)
                     /locus_tag="PLJ64_02935"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNC92480.1"
                     /translation="MKVAVISDIHANRHALDAVLADIDESGIEEIWFLGDAVGYGAFP
                     GECVLTLKDRCSVFLLGNHDLAALREIDIGTFSPSAAISARWTREHMDEDAFEVLRGL
                     DGTSAEREGIGLFHASPRDPIWEYVVDSDLAEDNLDFQEQRVALIGHSHIALYFTRVD
                     EMSRVSSVLAPEGTELEMSAGKWLLNPGSAGQPRDGDPRSAWAELDTESFQARFHRVQ
                     YPVEEAAEAIRKAGLPSHLGDRLFKGQ"
     gene            2406..3689
                     /locus_tag="PLJ64_02940"
     CDS             2406..3689
                     /locus_tag="PLJ64_02940"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNC92481.1"
                     /translation="MNSGSAAGRSLNGYALARVGLGAVLAACLAVLLLAPDQGPPVPD
                     PAAAARVIADRFSPHELDRADDYRSWGRLIGLASLVVQFATLAFLAFWRGAPMRRLLG
                     WLGRRPLPGALAAGALVSLVLALTGLPFDIAAWQLGRDYGLVNQALGERLLDWLLATV
                     VTAIPAALGGLLAMALWRRLKGRFWIAASFLVAIWAVITVWLWPVVISPLFNDFDPLP
                     DGPARTGVLRLAERAGVEVGEVYEVDASRRSSTLNAYVNGIGSTKQVVIYDNAIRTLN
                     RGEFTALVGHELGHVKAADLRRGLLFALLVIPLGVLFVQLATAAALRRNGDDLRSPAL
                     IPVIALWMALAAFALQIPGNALSRQVEAKADRYSIALTGDPQGLVDLQVRLAKANLSD
                     VDPPAFWQFMFGTHPSTFDRIAIAEGAKGGSRDEG"
     gene            3679..4125
                     /locus_tag="PLJ64_02945"
     CDS             3679..4125
                     /locus_tag="PLJ64_02945"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HNC92482.1"
                     /translation="MKVELIAVGKIKPPFAEAEAHYLKLLKPLMPVEVTETRDDDALG
                     RQVKSALATGGNRPDPGTIVALEAGGRDMTSERWAEWLDQRRHDGQRLTLLIGGPEGL
                     PGEVSGMADHRLSLGPQTMAHQLARVVLLEQLFRASKILAGQKYHL"
     gene            4165..5787
                     /gene="argS"
                     /locus_tag="PLJ64_02950"
     CDS             4165..5787
                     /gene="argS"
                     /locus_tag="PLJ64_02950"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNC92483.1"
                     /translation="MNSSADPTAALLEAVTVTARRLAGNEEISPALDPSPRPEMGDYS
                     TNAAMMLAKPLGRPPREIAAELAAAIEAELGPTLEKTEIAGPGFINLFLGDAWYREAT
                     AAMASTGAAEVPPVETPERIIVEFVSANPTGPLTAASGRHAAYGDSLSRLLRATGNGI
                     TTEYYVNDGGTQVQNFAASIAARMNGEEPPEDGYKGTYVNELAERAKAEGHDPADLEG
                     LARTGVGWMVEGIRGSLANFGVRMDDFFSERSLYEDNLVDRGIERMREAGHVFDEDGA
                     VWLKTTDFGDDKDRVLVRSTGEVTYFGADVAYHVDKIERGNDRLIDVLGADHHGYVAR
                     MAAAMEALEGGKAEFEAQIMQLIHLIEGGQRTKMSKRAGDIVTLDELTGDIGVDAARW
                     FLLQRSHNQTIDLDLDLARSQSSDNPVYYVQYAHARIQSILRKAAEEGADGGGDPLAA
                     PVDPSEKALVSKLLEFPAEVHEAAERRAPHRICAYASETAAAFHAFYRDCQVVGAEGE
                     GVEAARIQLCGVAAETIKRALELLGIGAPERM"
     gene            5839..7095
                     /locus_tag="PLJ64_02955"
     CDS             5839..7095
                     /locus_tag="PLJ64_02955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92484.1"
                     /translation="MKRLLFTALFGLMLLAGPAAAKAPTAGTLDRSFSGDGVVKLGED
                     GWAGWFSAVAGTPSGKVVGALSPGRGTRAGAVVRYLNRGGKDRSFGKRGFARLRFRSG
                     TINPVDVIVDRHGRILVAGLAAPNGRKGFWGALARLKRNGRPDRSFSGDGLAILPGTA
                     GVTPEEAFFSRGGRIVLAGETSEESALVMRLKANGRPDRRFSGDGRRLVRIAPDQSLG
                     GLAVTPAGKVVLGLSVRAESGSGRKGSIFGVVRLKPGGSLDRTFSKDGISLVNPTLDG
                     YSDYLSDLTVDRRDRIVAVGETTSLHSSMVRLLPDGRLDLLFAQLGREHLSWMNALGV
                     VVDDKGRIITSGYETPEGSDGEVHPQSPSGRLERLTTVGDFDRNFANGQYFYGLGESV
                     LDRRNRILVTASLGDSVSGIIRVRNP"
     gene            complement(7104..7571)
                     /locus_tag="PLJ64_02960"
     CDS             complement(7104..7571)
                     /locus_tag="PLJ64_02960"
                     /inference="COORDINATES: protein motif:HMM:NF015853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HNC92485.1"
                     /translation="MKRFGVLLLILFAFAGPALAIADEPNPRASLPQLEDEVMCPVCG
                     TLLGLSHSPAAERERVFIRKLIAEGKTDEEIKDALVAEYGGQVLALPENRGIDVWAYA
                     VPVIGLVLGLIGVIAAIVVWRRRRPDRGNEKPEAAPSPEEDDRLDEDMARFDL"
     gene            complement(7568..8167)
                     /locus_tag="PLJ64_02965"
     CDS             complement(7568..8167)
                     /locus_tag="PLJ64_02965"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNC92486.1"
                     /translation="MNWRTSIVVVGVVALLGFLVWGLVKKGDSSLAVGEPVPVATLPT
                     LPEGGEGSIEDYRGQWVLMNVWASWCGPCKAESPALDRFEEKNRDRIVVLGIDTKDLS
                     EDAMNFIREFNIKYPQLRDASGDYTDQKLKTTGVPESFLIDPEGNLVAHFPGPFEDEA
                     AIEEFAAPALESEANVDPAGVTGQNGPAGGSAKSEAASP"
     gene            8275..8347
                     /locus_tag="PLJ64_02970"
     tRNA            8275..8347
                     /locus_tag="PLJ64_02970"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8308..8310,aa:Gly,seq:ccc)
     gene            8382..9395
                     /locus_tag="PLJ64_02975"
     CDS             8382..9395
                     /locus_tag="PLJ64_02975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNC92487.1"
                     /translation="MIVAYESGPELGRTLPGLAGELAQGDEVIVVDNGSKVSPRDVVA
                     EHLPAATVIEMGENAGFTAATNRGAAASSGDLVLMLNPDAMPEPGFGEAIRRPFAAQP
                     DWGAWMALVACRIDGQKVVNSWSNPVHFTGICWAGGHGEPLERVGGKREIGVASGAAL
                     AVRREVWNQVGGLSEDFFLYQEDTDISLRIQAVGHRIGLQAEAVVDHDYDFHSNDRKW
                     FWLERNRLATVIRNYPAPLLALVAPALLGTELVLLAVSAKQGWLGAKLKSYRDLIRWL
                     PRLRRERAQIQSARVIPASQFADLLTPDLDSPLIPEFARKGLVRAMLRTYWRVVRTLL
                     ARR"
     gene            complement(9508..10212)
                     /locus_tag="PLJ64_02980"
     CDS             complement(9508..10212)
                     /locus_tag="PLJ64_02980"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNC92488.1"
                     /translation="MSEEKVLLITGASSGIGRATTRLMAGDRRLVLAGRRVEPLEELA
                     AELGGPERALVVGCDVGEWDQVETMTAAAIEAFGRIDEVFANAGFGASRGFLEESPEH
                     WKSMVQTNVLGTALTIRSTLPHLLERGSGHFIVTSSIAGRRVLPGSLYSATKFATTAI
                     GEALRQELRSRDDGKNIKVTLIEPGMVDTDFFENRPGESALKDEDIARTIQFALDQPE
                     GVDLNEILIRPISQAT"
     gene            10273..10674
                     /locus_tag="PLJ64_02985"
     CDS             10273..10674
                     /locus_tag="PLJ64_02985"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HNC92489.1"
                     /translation="MDVQRKRQVRLFVALGAALLLATGLIYTSFNASSEARQPSELVD
                     AVPGKTYEMTGKVVKDSVVKTGNGIRFKVKDRDGTGEALPVTYRGVVPDPFREGREIV
                     LTGTVENGVFVGEPDTLVTKCPSKFSEKQDQ"
     gene            10808..12865
                     /locus_tag="PLJ64_02990"
     CDS             10808..12865
                     /locus_tag="PLJ64_02990"
                     /inference="COORDINATES: protein motif:HMM:NF027651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HNC92490.1"
                     /translation="MVLCVVVIEHAFLTDDFSFNVVATHSSIETPAFYKFTAMWSSQE
                     GSLLLWAFILGIVSTISLAATRHKLRDLVPWATAVMMGIAMFFTGLMLLGGDVNPFTT
                     ISPVPVDGTGLNPLLQHPSMIIHPPMLYTGYVSLTVPFAFAIGALISRRVDAAWIKST
                     RGFALIAWTFLSIGIILGSRWSYTELGWGGYWAWDPVENASVMPWLITTAYIHSIMVQ
                     EKRGMLKVWNVSLIVASFSLALLGTFLVRSGVLQSIHAFGDSTVGPYFLSLIGVVLIG
                     STWLIISRLDILRSDRRIDSLASREAVFLINNLLLIALCAIVFWGTFFPLISELLTGT
                     RSTLAAPWFDRYTTPVAVVLVLFTGVGPMMSWRRISWEGAKRVFTVPLIAAAVAFVLL
                     LLFTDAAESIWALLIFTLAAFTVTALFQETWRAAAARKKSAGGTMLASIWSNATRNRR
                     RYGGYVVHLGLVVLLVGIAASSSFETKQDIELKPGQSAVVDDYTVTYLNPTLTRDRQA
                     LVFGAELQVDRGGKSFARLNPTRQYHQATDGSAPPGIASYFEGEATSEVGLKAGLRSD
                     FWTSVEPNTDEFQRRARLADGLFGAYMRKKVLPIVQADPAKAETVSRAAENLLSLTTD
                     KVIAGYNSDSIEVPVTIRVIVSPMVTWIWIGAVIAIIGALFAAWPSGLLTRKRGGKGR
                     KAA"
     gene            12862..13140
                     /locus_tag="PLJ64_02995"
     CDS             12862..13140
                     /locus_tag="PLJ64_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92491.1"
                     /translation="MTVVWFIVLLVLVAVFVSMPFWRRGEASGTEDPEIAALEAARES
                     KYREIRDAEMDRESGKLSEEDFRELDAELRREAVGILDRLEALKGKES"
     gene            13153..13380
                     /gene="secG"
                     /locus_tag="PLJ64_03000"
     CDS             13153..13380
                     /gene="secG"
                     /locus_tag="PLJ64_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNC92492.1"
                     /translation="MYTIMAIIQVLLSVLLIFLVLLHSGKDAGLSGAFGIGGGGSSIG
                     GGSMVEQNLDRATVVTAILFTLNTILLIKLS"
     gene            13415..13912
                     /locus_tag="PLJ64_03005"
     CDS             13415..13912
                     /locus_tag="PLJ64_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92493.1"
                     /translation="MTGKTKTTLAIIFGVVGILLGILGTITAFNAKNAVDDDKSQTSE
                     VAALVEQKFNEAQAKQDQAEANQRSEAEKLVAQLTQGKKNLLQKINTNHRQLKKVRRQ
                     VKNLSGQVSSLQSRDRELQNEIDQIENDQQADYNQLNGRINNLNRNVQQLQKQFARLN
                     GEING"
     gene            13921..14370
                     /locus_tag="PLJ64_03010"
     CDS             13921..14370
                     /locus_tag="PLJ64_03010"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HNC92494.1"
                     /translation="MRILFVCLGNICRSPSAEAVMAGLIVREGIEGIEIDSAGTGDWH
                     VGQPADPRAIAAATDRGVEISGLARQVRPSDFEEFDLILAMDSSNHANLLAMDGADPE
                     QVRLFREMAGEGELDVPDPYYGGEDGFAEVLDILESGCAVILEEINA"
     gene            complement(14385..15974)
                     /locus_tag="PLJ64_03015"
     CDS             complement(14385..15974)
                     /locus_tag="PLJ64_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92495.1"
                     /translation="MVRRPLPLAFLLLLLFLAPPARAADFPAGIEAGALTDPPLIASN
                     CERIREDGVGTSVINAVMTRIVKSPGVYDFASIDSQVNAAISCGIDPAMRVFAEPSSS
                     DERGMPDDLDAYGAFFGALVGHLKGRVGRFGVENEVISTTHWTDTPAGYFQLLRVAAD
                     ATHAANPDAIILDSVSSSGGFSIIEARKQWLAGDLEGALATIQESQTNDLGGGPYLTS
                     TSQLPAYLSNAKVMKMQEFYDQLVAHQDLIDALQLHYYGPASSIPATVAMVRADGFDM
                     PIEIWELGRRYLDGRPFQQHGHADESTRLITTAIGEGARYVVLQQYFDKPENEMYGLI
                     DGDGNSRQARFAVRNTLSMLRDTRSAERLRAGSGTDGYRFDRPGGWRRVLWATGDELK
                     PGKALGIRSTTVAVTGNQGGTTHLPVSSVTIGTSPQWFEPDMVEIWRQPARRKGFARV
                     KARCPVASPTRYCQGWIKLWGGKGNRTLVAGGKYRIARGTTRVTGIRRKRTRAKARVI
                     FATPFSCPGKLGNCRSWQKLP"
     gene            complement(16022..>17430)
                     /locus_tag="PLJ64_03020"
     CDS             complement(16022..>17430)
                     /locus_tag="PLJ64_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNC92496.1"
                     /translation="SIWAMNFAMMFALALGIDYALFIVYRFRGALTAHEDTGEAIAET
                     MDTAGKAVFFSGVTVLISLSAVLLVPSPAFRSMAIGVMLAVAFVLAASLTLLPGVLGK
                     LGHRVNRLSLPWVHTGEHRSPKFAAWGERIWARPLLFGIPALLILLALCLPVLSLDTG
                     MPSIKVVPEGDGARVGYEQVSEAFGPGAAGTLQVVAPGDDATRALASLRNDQGIARVM
                     VAGESPGGYTLIAATPGVDPSDPAMGTTIDRVRDALPPGTLVGGAAAENHDLEAALGD
                     TTAPAIAIVMTLGFLLLLIALRAPILAAIAVLTNLLAVGAAFGVAQLIFQQGHLTGLL
                     GIESQGFLDAWAPVFFFAMVFAISMDYSVFLLSSAREHWEKSGDAKEALIGALAHSGR
                     VVFAAAGVMVAVFFTFALSGPLPPREMGIILGVAVLLDAGLVRLMLLPVLMRLAGPGL
                     WWIPGWMDRRLPRVSFGH"
BASE COUNT         2895 a         5652 c         5824 g         3059 t
ORIGIN      
        1 cggtgagcgg tgcgggccgt gcgttccagc ggccgggtcg cggtctcggc ctgcgggaat
       61 tcggccggag ttaccggttc ctcgtccatc tggagagtca cgtcctcgcc ccgcaaccct
      121 cgctggagcc cgtcggaaag agccgcggcg gtgttgaagc ggtaggcctt gtcaccttcg
      181 agcgccctca tcaccgcggc gcttacggtt tccggcacgt cggcgttgta ggtcctcggc
      241 ggcagcggct tctcgttctg ctggcgcacc gcaagctcgg ccagcgagga accctcgtat
      301 ggcaggcgac cggtcaagaa ctggtagagc acgacaccca gggcgtaaac gtcggtcgac
      361 ggtgtcgcct cttcaccccg aacctgttcg ggggccaggt aggcggcggt cccgaccacg
      421 gagccgacct gggtgatccg ggtctgctcg gccgcccggg cgatcccgaa atcggtcagc
      481 ttgcaggtca tctcgccgcc gccgaccggg ccgccgatca ccatcaggtt gcccggcttc
      541 acgtcccggt ggatgatccc gtggcggtgg gcgtactcga ggccggcgca ggcctgcacg
      601 ccgatctcga ccgtcacctc gggatcgacc aggccttcgg tcttgaggat ctgggcgccc
      661 gacttgcctt cgacgcattc catgacgatg aagtggcggc catggtcgac accggtgtca
      721 tagacctgga cgatgttcgg atggatcagt ttggcgacgg ccagggcttc acgccggaaa
      781 cgggcgacga agcggtcgtc atccgagagg tgttcggcca gcaccttgac cgcaacggtc
      841 cgctccagga tgcggtcggt cgcccggtac acgtttgaca tgccgcctga gcccagcttg
      901 tcgcggagct cgtaacggtc agcgagaatc gtcggttcgg tcatcgtgga ttacccgggg
      961 ccgagcccgc cagagccagg gcctgtgctc ggtggtgtgg tcggggtcgt ggggttggtc
     1021 ggggtcgtcg gactggtcgg attggtgacc ggcggggtgg gctcggtctg ctggccgttg
     1081 tcgccgttct gggtctgatc cgtgttctgg ttttcatcgg tggtggtgcc ctgggtgccg
     1141 gtggtggtcg tttgggtgtc cgtggtgccg gtctctccgg tcgtgcctcc ggtcccggtg
     1201 tcggtttccg gcacttcggt ctggatcggc tcttccgtaa cggttccctc gtcgatgcag
     1261 atgtcctgat cactgagcat ggtctggagg gatgtcacac cgtcgatcag tgaacgtttc
     1321 agctccgggt cgattttgcc gccgagtcct tcgatctcgg cctgggccga agccgccacg
     1381 tcaccggccg caaaacactt gtgatcggcg gccagcgcct cggcacggtt gatcttggag
     1441 atcagcgaat tcgacgtttc ctcaggtatc aggttactcc gatcctcgcc acatccggcc
     1501 aggagcaggg agagggccag gctggcaaac agggcaggca gaaaaccccg gcgggggcgg
     1561 tgtcgggcaa aaaacggcat tcccgccgag tgtagatggt tggaccgctg gcctttgtga
     1621 cgcatcccgg acctttactg gcccttaaag agccggtcac ccagatggga gggaagaccg
     1681 gcctttcgga tcgcttctgc ggcctcttcg accgggtact gaacccggtg gaaacgggcc
     1741 tggaagctct ccgtgtccag ttccgcccag gctgaccgtg ggtcgccgtc ccgcggctgc
     1801 ccggcgctgc ccgggttgag cagccacttc ccggcgctca tctcgagctc ggtgccttcc
     1861 ggcgccagca cggacgaaac cctggacatc tcgtcaaccc gggtgaagta gagggcgatg
     1921 tgggaatggc cgatcagggc gacccgctgt tcctggaagt cgagattgtc ttcggccaga
     1981 tcgctgtcaa cgacgtattc ccagatcgga tctcgcggcg aggcgtgaaa aaggccgatc
     2041 ccctcccgct cggccgaggt tccatccagg ccacgcagaa cctcgaaggc gtcctcgtcc
     2101 atgtgttccc gggtccagcg ggcagatatc gcagccgagg ggctgaaagt gccgatgtcg
     2161 atctcccgga gcgcggccag gtcgtgattt ccgagcagga agaccgaaca gcgatccttc
     2221 agggtcagta cgcattcgcc gggaaaggcg ccgtagccaa ccgcatcccc aagaaaccag
     2281 atctcctcga tcccgctttc atcgatgtcg gcgagcacgg cgtccagtgc gtgccggttg
     2341 gcgtggatgt ccgagatgac ggcgactttc atggtcaggc ggcccgccga atatcgtatt
     2401 gagcaatgaa ttcgggttcg gccgcgggtc ggtcattgaa cggctacgcc ctggccaggg
     2461 tcgggttggg cgcggtgctg gcggcctgtc tggcggtgct tctgctcgct cccgatcagg
     2521 ggcctccggt tcccgatccg gccgccgcag cccgggtgat cgccgaccgg ttcagcccgc
     2581 atgaactgga ccgggccgac gattaccgtt cctggggtcg cctgatcggc ctcgcgtccc
     2641 tggtcgtcca gttcgccacc ctcgcctttc ttgctttctg gcgtggtgcc ccgatgcggc
     2701 ggctgctggg ctggctcggc cgccggccgc tgcccggcgc gctggccgcc ggcgccctcg
     2761 tttcccttgt tctggcattg accggtctgc cctttgacat cgccgcctgg caactcgggc
     2821 gggactacgg actggtcaat caggctctcg gtgagcgctt gctcgactgg ctgctggcca
     2881 ccgtggtcac ggcaattcct gctgcgctgg gcggactgct ggcgatggca ctttggcgaa
     2941 ggctgaaggg caggttctgg atcgcggcgt cgtttctggt cgcgatctgg gcggtgatca
     3001 ccgtctggct ctggccggtt gtcatctcgc cgctgttcaa cgatttcgac ccgcttcccg
     3061 acggcccggc gcgaaccggg gtgcttcgac tggcggaacg ggccggggtg gaggtggggg
     3121 aggtctacga ggttgacgca agtcgccgtt cctctacctt gaatgcgtac gtgaacggaa
     3181 tcggctcgac caaacaggtg gtcatctatg acaacgcgat ccggaccctg aaccggggcg
     3241 agttcacggc cctggtcggg catgagctcg ggcatgtgaa ggcggcggac ctgaggcgcg
     3301 ggctgctctt cgccctgctg gtcattccgc tgggggtgct cttcgtccag cttgccaccg
     3361 cggcggccct gcgccgcaat ggcgacgacc tccgcagtcc ggcgctgatc cctgtgatcg
     3421 ccctctggat ggccctggcg gcttttgcgc tccagattcc cggcaacgcg ctgtcccgcc
     3481 aggtcgaggc gaaggcggac cgttactcga tcgccctgac cggcgatccc cagggtctgg
     3541 tcgacctcca ggtgcggctc gccaaggcca acctgtcgga tgtcgatccg ccggccttct
     3601 ggcaattcat gttcggtacc catccctcga ccttcgatcg gatcgcgatc gccgagggtg
     3661 cgaagggagg cagccgggat gaaggttgaa ctgatcgcgg tggggaagat caagcccccg
     3721 tttgcggagg ccgaggccca ttacctgaag ctgttgaagc cgctgatgcc ggtcgaggtc
     3781 accgaaacca gggacgacga cgccttgggg cggcaggtga agtccgccct ggcgaccggc
     3841 ggaaaccggc ccgatccggg cacgatcgtc gcgcttgagg ccggcggacg ggacatgacc
     3901 tccgagcgct gggccgaatg gctggaccag cgccgccacg acggtcagag gctgaccttg
     3961 ctgatcggcg gtccggaagg tttgccgggg gaggtctcgg ggatggccga ccaccggctc
     4021 tcgctcggtc cgcagaccat ggcccatcag ctcgcccgcg tcgtcctgct ggagcagctc
     4081 ttccgggcca gcaagatcct cgccggccag aagtaccacc tctagtgaca gcggccggcc
     4141 gggcccgcaa atagaatcgc cggcgtgaac agttccgccg atcccaccgc cgctctgctt
     4201 gaggccgtta ccgtcacggc ccggcggctg gccggcaatg aagaaatcag cccggccctt
     4261 gacccgtcgc cccgccccga aatgggcgac tactcgacca acgcggcgat gatgctggcc
     4321 aagccactgg gccggccacc tcgcgagatc gccgcggagc tggccgccgc gatcgaggcc
     4381 gagctgggcc cgactctcga gaagaccgag attgccggcc ccggtttcat caacctcttc
     4441 ctcggtgacg cctggtaccg cgaagctacc gccgccatgg cctcgaccgg agcggccgaa
     4501 gtgccgccgg tcgaaacccc ggaacggatc atcgtcgagt tcgtctccgc caatccgacc
     4561 ggccccctga ccgccgcttc gggccggcac gctgcctacg gcgactcgct ttcacgcctg
     4621 cttcgagcca ccggcaacgg gatcacaacc gagtactacg tcaacgacgg tggcacccag
     4681 gtccagaact tcgccgcctc gatcgccgcc cgcatgaacg gggaggagcc ccccgaggac
     4741 ggctacaagg gcacctacgt gaacgagctg gccgagcggg cgaaggccga aggtcacgac
     4801 ccggccgatc tcgaaggcct ggcccggacc ggggtcgggt ggatggtcga gggcatccgc
     4861 ggttcgctgg cgaacttcgg ggtccggatg gacgatttct tctccgagcg ctcgctctat
     4921 gaagacaacc tggttgaccg cggcatcgag cggatgcgcg aagcgggcca tgtcttcgac
     4981 gaggacggcg cggtctggct gaagaccacc gactttggcg acgacaagga ccgggtcctg
     5041 gtcaggtcca cgggcgaggt cacctacttc ggggccgatg tggcctacca cgtcgacaag
     5101 atcgagcggg gcaacgaccg gctgatcgac gtgctcggag ccgaccacca cggttacgtg
     5161 gcacggatgg ccgcggcaat ggaagcactc gaaggcggca aggccgagtt cgaagcccag
     5221 atcatgcagt tgatccacct gatcgagggc ggccagcgga ccaagatgag caagcgggcc
     5281 ggggacatcg tcacccttga cgagctgacc ggggacatcg gcgtcgacgc cgcccgctgg
     5341 ttcctgcttc agcggagtca caaccagacc attgatctcg atctcgacct ggcccgcagc
     5401 cagtcgtcag acaacccggt ctattacgtc cagtacgccc acgcccggat ccagagcatc
     5461 ctccgcaagg cggccgagga aggtgccgac gggggtggtg atccgctcgc cgctccggtc
     5521 gatccttcgg agaaagcgct cgtttcgaag ctgctcgagt tcccggccga ggttcacgaa
     5581 gcggcagaaa ggcgcgcccc gcaccggatc tgcgcctatg ccagcgagac cgccgccgcc
     5641 ttccacgcct tctaccgcga ctgccaggtt gtgggggctg aaggagaggg ggtcgaggcc
     5701 gcccggatcc agctctgcgg cgtcgctgcc gaaacgatca aacgcgcact ggaactgctc
     5761 ggaatcggcg ctcccgagcg gatgtgaggg tgctgcacgg aaccgaatcg ttccgtgtta
     5821 cccggtgttt ttgaggtcat gaagaggctg cttttcacgg cgcttttcgg gctcatgctg
     5881 ttggccgggc cggccgctgc caaggccccg accgcgggaa cgctggaccg gagcttttcc
     5941 ggtgacggcg tcgtcaaact cggcgaggac ggctgggcgg gctggttctc ggcggtcgcc
     6001 gggacgccgt cgggcaaagt ggtcggtgcc ctttctccgg gccggggaac cagggccggg
     6061 gccgtggtca ggtacctaaa ccgcggcgga aaggaccggt ctttcggcaa gcgcggattc
     6121 gcccgactga ggtttcgctc cgggacgatc aacccggtcg acgtgatcgt cgaccggcat
     6181 ggccgcatcc tggtcgccgg ccttgccgca ccgaacggac gcaagggctt ctggggcgct
     6241 ctggcaaggc tgaagagaaa cggccggccc gaccggagtt tctccggcga cggcctggcg
     6301 atcctgccgg gaactgccgg cgtcactccc gaggaggcct tcttctcccg cggcggccgg
     6361 atcgtcctcg ccggcgagac cagcgaggaa tccgcgctgg tcatgcgcct caaggcaaac
     6421 ggaaggccgg accgccggtt ctccggtgac gggcgccggc tggtccggat tgctccggat
     6481 cagagcctgg gcgggctcgc agtgactccg gccggcaaag tcgtgctcgg gctttcggtc
     6541 agggcggaat ccggttccgg gaggaaggga agcatcttcg gggtggttcg cctgaaaccc
     6601 ggcgggagcc tggaccggac tttctcgaag gacggaatct ccctcgtcaa cccgaccctg
     6661 gacggttact cagattacct gtccgacctg actgtggacc gccgtgaccg aatcgtcgcc
     6721 gtgggtgaaa ccacttcgct tcacagcagc atggtccggc tgttgccgga cggccgcctt
     6781 gatctgctct tcgcccagct cggccgcgag catctttcct ggatgaacgc gctcggggtc
     6841 gttgtcgacg acaaggggcg gatcatcacc tccggatacg agacaccgga agggtccgac
     6901 ggagaggttc acccgcagag cccgagcggc cgtctggaac ggctgaccac cgttggagac
     6961 ttcgaccgga atttcgccaa cggccagtac ttctacggcc tgggagaatc ggttctggac
     7021 cgcaggaacc ggatcctggt caccgcctcc cttggtgatt cggtcagcgg aataatccgc
     7081 gtccgcaatc cctgaccgga cggtcagagg tcgaagcggg ccatgtcctc gtcaaggcgg
     7141 tcgtcctctt cgggactggg cgccgcttcg ggtttctcgt tcccgcggtc gggccggcgg
     7201 cggcgccaga cgacgatcgc ggcgataacg ccgatcaggc cgaggacgag accgatcacc
     7261 gggaccgcgt aggcccagac gtcgatgccg cggttctccg gcagggcgag aacctgaccg
     7321 ccatattcgg cgaccagggc atccttgatc tcctcgtcgg tcttgccctc ggcgatcaat
     7381 ttgcggatga agacgcgttc gcgttcggcg gccggggagt gggagagacc gagcagggtg
     7441 ccacagaccg ggcacatcac ctcgtcctcg agctgcggca ggctggcccg cggattcggt
     7501 tcgtcggcga tcgccagggc cggcccggca aaggcgaaca gaatcagcag caggaccccg
     7561 aagcgcttca cggggaggct gcctctgact tggctgaccc tcccgccggg ccgttttgcc
     7621 cggtgacgcc ggccgggtcc acgttcgcct ctgactcgag ggcgggtgcg gcgaactctt
     7681 cgatcgccgc ttcgtcctcg aaggggccgg ggaagtgagc gaccagattc ccttcggggt
     7741 cgatcaggaa ggattcgggc accccggtgg tcttcagctt ctggtcggtg tagtcgccgc
     7801 tggcgtcacg aagctgcggg tacttgatgt tgaactccct gatgaagttc atcgcgtcct
     7861 cgctcaggtc cttggtgtcg atcccgagca cgacgatccg gtcgcggttc ttctcttcga
     7921 atcggtcgag cgccggcgac tctgccttgc aggggccgca ccaggaggcc cagacgttca
     7981 tcaacaccca ctggccgcgg tagtcctcga tcgagccttc gcccccctcg ggcagggtcg
     8041 gcagggtggc aaccggcacg ggttcgccca cggcaaggct cgagtcacct ttcttgacca
     8101 gtccccagac gaggaagccg agcagcgcga caacgccgac cacgacgatg gaagttcgcc
     8161 agttcacgat ggtcgattat ggagaaccgg gcccggcggc cgccccgcgc ccacgctgaa
     8221 ccaccaccgg cgggcggagt caatctgtga cggggtaagg ttccaagccg cttcgcggac
     8281 gtagctcagt tggtagagcg cgagcttccc aagctcgagg tcgcgggttc gagacccgtc
     8341 gtccgctttt gaccgaacac cgcctaccga ctctctcggt cgtgatcgtg gcctacgaat
     8401 ccgggcccga actggggcgg acgctgcccg gactggccgg tgaactggct cagggggatg
     8461 aggtgatcgt ggtcgacaat ggttcgaagg tctcgccgcg ggatgtcgtg gccgagcatc
     8521 tgccagcagc gacggttata gagatgggcg aaaacgccgg tttcaccgcg gcgaccaacc
     8581 gcggggctgc cgcttcaagc ggggatctgg tgctgatgct gaaccccgac gccatgcccg
     8641 agcccggctt tggcgaggcg atccgccggc ccttcgcagc gcagccggac tggggcgcct
     8701 ggatggcgct ggtggcctgt cggatcgacg ggcagaaagt cgtgaattcc tggtccaacc
     8761 cggttcattt cacgggcatt tgctgggccg gcgggcacgg ggagccgctc gaacgcgtgg
     8821 gagggaaacg cgagataggt gtggcatccg gcgctgcgct ggcggtgagg agggaagtgt
     8881 ggaaccaggt cggcggcttg tccgaagact tcttcctcta ccaggaagac accgacattt
     8941 ccctgcgcat tcaggccgtc gggcacagga tcggtttgca ggccgaagcg gtagtcgacc
     9001 acgactacga ctttcattcc aacgaccgga agtggttctg gctcgagcga aaccggctcg
     9061 cgacggtgat ccgcaactac ccggccccgc tgctcgccct ggttgccccg gccctgctcg
     9121 gcaccgaact ggtgctattg gcggtcagcg cgaaacaggg gtggctcggg gccaagctga
     9181 agtcctaccg cgacctgatc cgctggcttc cccgcctgag gcgggagcgg gcgcagatcc
     9241 agtcggcccg ggtgatcccg gccagccagt tcgccgacct cctgacaccc gacctcgatt
     9301 cacccctgat tcccgaattc gcccgaaaag ggttggtgag ggcaatgctc cgcacctact
     9361 ggcgagttgt ccggaccctc ctcgccagga ggtagccatg gagcccgatc atcacttgtt
     9421 gatgatcgtg atcgggcggg tgcctgctcg aagtccggat ccccaaccgg tgtgaaagct
     9481 gtgggagcgc cgaaggcgcg actgcagtta agtcgcctgg gagatcgggc ggatcaggat
     9541 ctcgttcagg tcgacgcctt caggctggtc gagggcgaac tggatcgtgc gggcgatgtc
     9601 ttcgtccttg agggccgatt cgccggggcg gttctcgaag aagtccgtgt caaccatgcc
     9661 cggctcgatc agggtcacct tgatgttctt gccgtcatcc cggctccgca gttcctgccg
     9721 cagcgcttcc ccgatcgcgg tggtcgcgaa cttggtcgcc gagtagagcg agccgggcag
     9781 gacccggcgg ccggcgatcg agctggtcac gatgaaatgg ccgctgcccc gttcgagcag
     9841 gtggggcagg gtggaacgga tggtcagggc ggtcccgagc acgttggtct ggaccattga
     9901 cttccagtgt tccggtgact cctcgaggaa gccgcgactg gccccgaagc cggcgttggc
     9961 gaagacctcg tcgatccggc cgaatgcctc gatcgccgcg gcggtcatgg tctcgacctg
    10021 gtcccattcc ccgacgtcgc agccgacgac cagggcccgc tcgggcccgc ccagttccgc
    10081 cgccagttcc tccagcggct cgacccggcg gccggccaga accagccgcc tgtccccggc
    10141 catcaggcgg gtcgtggccc tgccgatgcc gctgcttgct ccggtgatca gaagtacctt
    10201 ctcttcgctc atgcttcgat tctgtcaggg ggaagccgcc ggccgccaag gcttgcgata
    10261 atcccttccg ttatggatgt acagcgcaag cggcaggtgc gcctgttcgt ggctctgggc
    10321 gccgccctcc ttctggccac cggactgatt tacacctcct tcaatgcttc ttccgaggcc
    10381 cgtcagccca gtgagctggt cgatgccgtg cccggaaaga cctatgagat gaccggcaag
    10441 gtggtcaagg actccgtagt gaagaccggg aacggcatca ggttcaaggt gaaggaccgt
    10501 gacggaaccg gcgaggcgct gcccgtcacc tacagagggg tcgtgcccga ccccttccgc
    10561 gaagggcgcg aaatcgtgct cacgggcact gtcgaaaacg gggtcttcgt gggggaaccg
    10621 gacacgctcg tgaccaagtg cccctcgaag ttctcggaga agcaggatca gtaacttagg
    10681 caacgcggcg ctctgggttg ccctgctgac cgcgacattc ggggcggtcg cagcgctggc
    10741 cggaaccagg ggcgaccgac gctgggttga ctcctcccgc tacgcggtct acgccttctg
    10801 cgccctgatg gtgctctgcg tggtcgtgat cgaacacgcc ttcctgaccg acgacttcag
    10861 cttcaacgtg gtcgccaccc actcgtcgat cgaaactccg gccttctaca agttcacggc
    10921 gatgtggtcg agccaggaag gctcgctgct gctctgggcc ttcatcctcg ggatcgtctc
    10981 gacgatttcc ctggccgcga cccggcacaa gctgcgggac ctcgtgccct gggcgaccgc
    11041 ggtgatgatg ggcatcgcga tgttctttac cggcctgatg ctcttgggcg gcgatgtcaa
    11101 tccgttcacg acgatctcgc cggttccggt cgacggcacc gggctcaacc cgctgcttca
    11161 gcatccctcg atgatcatcc acccgccgat gctctacacc ggctatgtgt cgctgaccgt
    11221 tcccttcgcc ttcgcgatcg gcgccctgat cagtcgccgg gtggacgcgg cctggatcaa
    11281 gtcgacccgc ggctttgcgc tgatcgcctg gaccttcctc tcgatcggca tcatcctcgg
    11341 ctcgcgctgg tcatacaccg agctcggttg gggcggttac tgggcctggg atccggtcga
    11401 aaacgcctcg gtcatgccct ggctgatcac caccgcctac atccactcga tcatggtcca
    11461 ggagaagcgg ggcatgctga aggtctggaa cgtgagcctg atcgtcgcca gtttcagcct
    11521 cgccctgctc ggcaccttcc tggtccgttc cggtgtgctc cagtcgatcc acgctttcgg
    11581 cgacagcacg gtcgggccct acttcctctc gctgatcggc gtggtcctga tcggttcgac
    11641 ctggctgatc atctcccgcc tcgacattct ccgctcggat cgaagaatcg actcgctggc
    11701 ctcccgcgag gcggtgttcc tgatcaacaa cctgctgctg atcgcccttt gcgcgattgt
    11761 cttctggggc accttcttcc cgttgatctc cgagcttctg accggcaccc gttccaccct
    11821 tgccgctccc tggttcgacc gctacacgac cccggtcgcg gtcgtgctcg tcctcttcac
    11881 cggagtcggg ccgatgatga gctggcgccg gatctcctgg gagggagcga agcgcgtctt
    11941 cacggtcccc ctgattgccg cggcggtcgc cttcgtcctg cttctgcttt tcaccgatgc
    12001 cgcggaaagc atctgggcgc tactgatctt caccctcgct gccttcacgg tcaccgcact
    12061 gttccaggaa acctggcgcg cggccgcggc caggaagaag agcgccggcg gcacgatgct
    12121 cgcttccatc tggtccaacg cgactcgcaa tcgccggcgc tacggcgggt acgtggtcca
    12181 ccttggcctg gtcgtgctgc tggtcgggat cgccgcctca tccagcttcg aaacgaaaca
    12241 ggacatcgaa ctgaagcccg gccagtccgc ggtggttgac gattacaccg tcacctatct
    12301 gaatcccacc ctgacccgcg acaggcaggc cctcgtcttc ggagccgagc tccaggttga
    12361 ccgcgggggc aagtccttcg cccggctgaa cccgacccgg cagtaccacc aggccaccga
    12421 cggcagcgcg ccgcccggca tcgcctccta cttcgagggg gaagcgacca gcgaggtcgg
    12481 cctgaaggcc gggctcaggt cggacttctg gacctcggtc gagccgaaca cggacgagtt
    12541 ccagcgtcgg gcgaggcttg ccgacggact cttcggggct tacatgcgca agaaggtcct
    12601 gccgatcgtt caggccgatc ctgccaaggc ggaaacggtc agccgtgcgg cggagaacct
    12661 gctgagcctg accaccgaca aggtgatcgc cggatacaac tcggacagca tcgaggtgcc
    12721 ggtgacgatc cgggtgatcg tcagcccgat ggtcacgtgg atctggatcg gcgccgtgat
    12781 cgcgatcatc ggtgcgcttt tcgcggcctg gccgagtgga ctcctgaccc ggaaacgggg
    12841 cgggaaggga aggaaagccg cgtgaccgtc gtctggttca tcgtcctgct ggtcctggtc
    12901 gcggtcttcg tctcgatgcc gttctggcgt cgcggcgagg cttccggcac cgaggatccc
    12961 gaaattgccg ctctcgaagc ggcccgggag tcaaagtacc gggagatccg cgacgccgag
    13021 atggaccgcg agtccggcaa gctctcggag gaggatttca gggaactcga cgccgaactg
    13081 agacgggaag cggtcggcat tctcgaccgc ctcgaggcgc tcaagggcaa agaaagctag
    13141 aatcgctggc cgatgtacac gatcatggca atcatccagg tcctgctttc cgtgctcctg
    13201 atcttcctgg tgctcctgca ttcgggcaag gacgcgggtc tctccggcgc tttcggcatc
    13261 ggtggcggcg gcagttcgat cggcggcggc tcgatggtcg agcagaatct cgaccgggcg
    13321 acggttgtca ccgcgatcct gttcaccctg aacacgatcc tcctgatcaa gctttcttaa
    13381 gccggatccc cgttgttggt ctagggtcag cgccatgact ggcaagacca agacgacact
    13441 ggcgatcatt tttggcgtag tcggaatcct gctcgggatt ctcggcacga tcaccgcttt
    13501 caacgcgaag aacgcggttg acgatgacaa gagccagacc agcgaagtcg cggctctggt
    13561 cgagcagaag ttcaacgaag cccaggccaa gcaggaccag gccgaagcca accagcgttc
    13621 agaggccgag aagctggtcg cccagctgac ccagggcaag aagaacctgc tccagaagat
    13681 caacacaaac cacaggcagc taaagaaggt tcgtcggcag gtgaagaacc tctcggggca
    13741 agtcagttcg ctccagagcc gggaccgcga gcttcagaac gagatcgatc agatcgaaaa
    13801 cgatcaacag gctgactaca accagctgaa tggccggatc aacaacctca acaggaacgt
    13861 tcagcagctg cagaagcagt tcgcccggct gaacggcgag atcaacggct gagcccggac
    13921 ttgcggatcc ttttcgtatg cctcggcaac atctgccgtt cgccctcggc cgaggcggtg
    13981 atggccgggc tgatcgtgcg cgaaggaatc gagggaatcg agatcgattc ggccggcacc
    14041 ggcgactggc atgtcggcca gccggccgat ccgcgggcga tcgcggccgc gaccgaccgc
    14101 ggggtcgaga tcagcggcct cgcccgccag gtccggccct cggacttcga ggagttcgac
    14161 ctgatcctcg cgatggacag ctccaaccac gcgaatctgc ttgcgatgga cggagccgac
    14221 cccgaacagg tgaggctctt ccgggaaatg gcgggcgaag gagagcttga cgtgcccgac
    14281 ccctactacg ggggcgagga cggtttcgca gaggtgctgg acatcctcga gagcggctgt
    14341 gccgtgattc tcgaagagat caacgcctga ccgcgccgcg cgggtcaggg cagtttttgc
    14401 cagctgcggc aattcccgag cttgccgggg cagctgaacg gtgtggcgaa gatgacccgc
    14461 gccttcgccc ttgtccgctt gcgccggatc ccggtgacac gggtcgtgcc ccgtgcgatc
    14521 ctgtacttcc cgccggcgac cagggtccgg ttgcccttgc ctccccacag cttgatccag
    14581 ccctggcagt accgggttgg tgacgcgacc gggcagcggg ccttgacccg ggcgaagccc
    14641 ttgcgcctgg cgggctggcg ccagatctcg accatgtccg gttcgaacca ctgcggcgag
    14701 gttccgatcg tgaccgagga gaccggcagg tgggtggtgc cgccctggtt gccggtcacg
    14761 gcgaccgtgg tcgagcggat gccgagcgcc ttgcccggtt tcaactcgtc gccggtcgcc
    14821 cagagaaccc gccgccagcc gccgggccgg tcgaaccgat aaccatcggt gcctgaaccg
    14881 gcccggagcc gctcggcgga tctggtgtcc ctcagcattg aaagcgtgtt gcggacggcg
    14941 aaacgggcct ggcgcgagtt gccatcaccg tcgatcagtc cgtacatctc gttttcgggc
    15001 ttgtcgaagt actgctggag caccacgtaa cgggcgcctt cgccgatcgc ggtggtgatc
    15061 agccgggtcg actcgtcggc gtgaccatgc tgctgaaacg gtcgtccgtc caggtacctg
    15121 cgaccgagct cccagatctc gatcggcatg tcgaaaccgt cggccctgac catcgcgacg
    15181 gtcgccggaa tgctggacgc gggcccgtag tagtggagct gaagggcatc gatcaggtcc
    15241 tgatgggcga ccagctggtc gtagaactcc tgcatcttca tgaccttggc gtttgaaagg
    15301 taggccggca gctggctggt cgaagtgagg taggggccgc cgccgaggtc attggtctgg
    15361 ctttcctgaa tcgtggccag ggcaccttcg agatcgccgg ccagccactg cttgcgggcc
    15421 tcgatgatcg aaaagccgcc gctcgagctg accgaatcga ggatgatcgc gtcggggttg
    15481 gccgcatggg tcgcgtcggc cgcgacccgg agcaactgga aatacccggc gggagtgtcg
    15541 gtccagtggg tcgtcgagat aacttcgttt tcgactccga aacgacctac ccgccccttc
    15601 aggtgaccga ccagggcacc gaagaacgca ccgtaggcat cgaggtcatc cggcatgccg
    15661 cgctcgtcgc tgctggaggg ttcggcgaag acccgcatcg ccgggtcgat tccgcaggag
    15721 atcgcggcgt tgacctggga gtcaatcgat gcgaagtcgt aaaccccggg ggatttcacg
    15781 atccgggtca tgaccgcgtt gatcaccgaa gtcccgacgc cgtcttccct gattcgctcg
    15841 cagttcgagg cgatcagcgg cgggtccgtc agcgctccgg cctcgatccc ggcggggaag
    15901 tcggcggccc gcgccggcgg ggccaggaac aggaggagca gcaggaacgc cagggggagt
    15961 ggacgccgga ccattacctt gggaacccgg cagccggtgg ccggttgcgc tcctgcccgg
    16021 atcagtgacc gaaactgacc cgaggcaggc gcctgtccat ccagcccgga atccaccaga
    16081 gcccgggccc ggcgagccgc atcaggaccg gcagcagcat caacctgacc agacccgcgt
    16141 ccagcagcac ggcgacaccg aggatgatgc ccatctcgcg cgggggcagg gggccggaga
    16201 gcgcgaaggt gaagaacacg gcgaccatga cgccggcagc cgcgaacaca acccgtccgg
    16261 aatgggccag cgcaccgatc agggcctcct tcgcgtcacc ggacttttcc cagtgttcgc
    16321 gggccgagct gagcaggaac acggaatagt ccatcgagat cgcgaagacc atcgcgaaaa
    16381 agaagaccgg tgcccaggcg tcgaggaagc cctgggattc gatgcccagc agcccggtca
    16441 ggtgaccctg ctggaagatc agctgggcca cgccgaaggc ggcaccgacc gccagcaggt
    16501 tggtcagcac cgcgatcgcg gccaggatcg gggctcgcag cgcgatcagc agcaggagga
    16561 agccgagggt catgacgatc gcgatcgccg gcgcggtggt gtcacccaaa gcagcttcga
    16621 ggtcatggtt ctcggccgcg gctccgccga ccagggtgcc gggcggcaat gcgtcacgga
    16681 cccggtcgat cgtcgttccc attgccggat cggatggatc cacgcccgga gtcgccgcga
    16741 tcagggtgta gccaccggga ctttcgccgg ccaccatgac ccgggcgatc ccctgatcgt
    16801 tccggaggga ggcgagcgcc ctggtggcgt cgtcaccggg cgcgactacc tgcagggttc
    16861 cggccgcccc gggaccaaag gcttcggaga cctgttcgta gccgacccgg gctccatcgc
    16921 cttccggcac gaccttgatc gaaggcatgc cggtgtccag cgaaaggacc ggcaggcaaa
    16981 gggcaagcag gatcagcagc gccggaatgc cgaagagcag tggccgggcc cagatccgct
    17041 cgccccaggc ggcgaacttc ggcgagcgat gctcgccggt gtggacccag gggaggctga
    17101 gccggttgac ccgatgaccc agcttgccaa gcacgccggg cagcagggtc agtgacgcgg
    17161 ccaggacgaa ggcgaccgcc agcatcaccc cgatcgccat cgagcggaaa gccggactcg
    17221 gcaccagcag cacggcgctg agtgaaatca gcacggtcac gccggaaaag aagacggctt
    17281 tcccggccgt gtccatcgtt tcggcgatcg cctcgccggt gtcttcgtgt gcggtcagcg
    17341 caccacggaa ccggtagacg atgaagaggg cgtagtcgat gccgagcgcg agggcgaaca
    17401 tcatcgcgaa gttcatcgcc cagatcgaga
//
LOCUS       DAOQKN010000025        17390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_1474_length_17390_cov_6.120335, whole genome shotgun sequence.
ACCESSION   DAOQKN010000025 DAOQKN010000000
VERSION     DAOQKN010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 17390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17390
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1474_length_17390_cov_6.120335"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(342..821)
                     /locus_tag="PLJ64_03025"
     CDS             complement(342..821)
                     /locus_tag="PLJ64_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC92497.1"
                     /translation="MLDREYDNQVCSIARSLEVVGERWSLLIIRNVGLGLNRFEPLRE
                     NLGITRSVLTTRLNTLIEYGILEKRQYSEKPPRFEYHLTQKGRDLSPVLLQLMWWGDE
                     YYPEPGGPPTIAVHRTCGGNLDHHMVCEKCGESVEPDRVYMKPGPGRRRQDLPKIPA"
     gene            909..2399
                     /locus_tag="PLJ64_03030"
     CDS             909..2399
                     /locus_tag="PLJ64_03030"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC92498.1"
                     /translation="MSDTVTGKFEAVEEEILVGAPTPAAGPVPKEMAEAEAAYRPEVL
                     GKVLAVVAAAVALASLDMFIVNIAFPAIEADFPGSSIGSLSWVLNGYVIVFAALLMPF
                     GRLADRVGRKRIFTTGVLIFCLASGACAAAWSVETLVAFRVIQGIGAAMLMSTSLALL
                     LHAFPAERWPVVIGVWAAAGGAAGALGPPIGGLLVEISWRWVFLVNLPLSLAAWYFGT
                     RLLVESKDPRETRWPDPAGMALMILGIGVLCWGLVEAPDHGWGSIRTVVAILAGLVML
                     GLTFLRSRRPADRWIPTLDVDLFKARPFAMACLTALIFMVAFAAILLGGVLFLTTVWG
                     FSVLEAGIAFFPGPAMAGIFSVLGSRFGARFGTGPVAATGCGFLILGGTWWFTRMGDT
                     SAYFTENLPGQILVGIGVGLVLPNVAAAVASTLPPTSLATGTAVLSASRQVGAALGVA
                     ILVALLGSAAVVGTADDFDKAWLMIAISAAIAGATALTIGRAGKTE"
     gene            2415..3407
                     /gene="trpS"
                     /locus_tag="PLJ64_03035"
     CDS             2415..3407
                     /gene="trpS"
                     /locus_tag="PLJ64_03035"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNC92499.1"
                     /translation="MRIFSGIQPTGRKHLGNYIGAIRQYVEGQDRGDPAIYSIVDLHA
                     ITVAYEPGTLRTNLYDTTAAILAAGVDPERCILFRQSDIPEHTELCWLLSGVCAHGDL
                     NRMTQFKEKSAQQKDLVSAGIFIYPLLQAADVLAYQADEVPVGEDQRQHIELMREVAR
                     RFNERFGETFTIPEHRIPEIGARVMDLQEPGRKMSTSVGFDSGRVYLLETEKETRKKL
                     GSAVTDSGREVKRGEGKEGVANLIGIYAVATGKTEDEVEAEFEGAGYGDFKKAVADAV
                     VEFLAPVREAYPAIREDEGEIERILADGAAKAREIASVTLADVREAMGVGVPRG"
     gene            3488..4249
                     /locus_tag="PLJ64_03040"
     CDS             3488..4249
                     /locus_tag="PLJ64_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HNC92500.1"
                     /translation="MASRLDLDLDLDLDVFIGPFDLLLSIVLREEISLLEVELGEIVA
                     MYVGKLEEEGELELEVATEFLVLIAALLELKSRLMLPGADEDLTDMEPAEAVEELVAR
                     MLEYRRYRDAGRALKEHFESQRGFLYRSAPLPPELRRVEVETAEKVYEPEQLAAALGD
                     LLEPPPRPDLSHLRATVSLRRRLSVVRSVLMNRPVFDFDDEFGNEDRMTQAVTIFALL
                     DLYKKGEAVWEQTESCGPITVKRIQAGQNGLARTA"
     gene            4246..4818
                     /gene="scpB"
                     /locus_tag="PLJ64_03045"
     CDS             4246..4818
                     /gene="scpB"
                     /locus_tag="PLJ64_03045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNC92501.1"
                     /translation="MTDLARRIESLLFLSNQPVTEIALAEACEVELEQVQNAVVELEE
                     DLEPGRRGLILRRVGGGLILASDPESEAAARRLLSKPRTPPLTQAQAETLAIVAYLQP
                     VARPEIARIRGVSSESAVNSLSERGLIEESGRSRFGAAIYRTSPLFEKVFGLEGLDAL
                     PDPTRFDPTPEDEREIRERLLAAGEQRSEA"
     gene            4904..6061
                     /locus_tag="PLJ64_03050"
     CDS             4904..6061
                     /locus_tag="PLJ64_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92502.1"
                     /translation="MKKFAVLLALLASVFAVAACGSNNNPTVTPDTLASAMDEVCTDT
                     NADFEAMGTRGLTMDGIAAEFEGYAEVRQAVVDGFNELNTDDQAEPQLQKYIDASEQV
                     IAQDKAIAKAAAEGDQKAMNEAFAAQTKAFNERDKVAKEIGTEVCGQTPEIKVEKTGT
                     APPSDLNVVEPKNTVEDAADNYIEATASGNCGLINANRHTDAGDLDEASCKAAGQALK
                     GARVIGTESYGPVAQAEILSGNGTHYPTYFVTDLDGNLRYGGDAIHDSGGLRPAPEGN
                     DAEATVEATVNAIRDNDAAAFNETLAFEDSPFWIKGDTVEKFGEGAYNEEFIKDVKDG
                     DADPIQLGLNSTWAFYFLEGSKYDWVLSFTHNPGIGGHYAFTGYYPIPKPN"
     gene            6084..6782
                     /locus_tag="PLJ64_03055"
     CDS             6084..6782
                     /locus_tag="PLJ64_03055"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNC92503.1"
                     /translation="MRLAKYLAHAGVASRRKSEEIVAAGRVRVAGKVITDPAFGVEGG
                     EEVRVDGHQVEPEGREVWMLNKPLDVISTADEPGKRIAVVDLVDSNARLYPVGRLDAD
                     STGLILLSNDGELANRLTHPRFEVEKTYRVKLKKPVTPEAIDQLQRGVRLDDGMTAPA
                     TVKKLGEAELDVTIHEGRNRQVRRMAEAIGNEVVALQRISLGSLKLGRLGIGKCRLLD
                     EREVERLWKDAGDD"
     gene            6775..7161
                     /gene="aroH"
                     /locus_tag="PLJ64_03060"
     CDS             6775..7161
                     /gene="aroH"
                     /locus_tag="PLJ64_03060"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HNC92504.1"
                     /translation="MTDPEAEGMRVAAIRGAVQAENDADVILSATEELIREIISVNDL
                     EPDRMISAIFTTTEDLDAEFPAAAARRMGLNEVPLMCAREIPVPGAMPGVIRVMLHTY
                     LPAGAKARHVYLGETRNLRADLDAAQ"
     gene            7190..8302
                     /gene="hisC"
                     /locus_tag="PLJ64_03065"
     CDS             7190..8302
                     /gene="hisC"
                     /locus_tag="PLJ64_03065"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNC92505.1"
                     /translation="MTVRFNSHLTSIPGYSPGVPKGHSAEDVASSNLAQLASNESPFA
                     PLPEVVEAITRAAKAMNRYPDPAATRLRQRLADRHEIEPGQVSVANGSCEFLLAAAEA
                     LLEPGAEIVYAWPSFSMYPNMAAMTGAREIRVPLKEDQTHDLEAMLAEITAATQIVIV
                     CNPNNPTGTYIPFAEIADFVERVPDHVQIILDEAYIEFQVYDDPANSIDLLKRHPNLV
                     LLRTFSKTHSLAGLRVGYALGSPAFKAAVEAVRQPFSVNALAQAAAVEALEHGDVIQR
                     QIETQIAERVRVESGVADLGLRATDSQANFSWISLGDNGAEIEDAVVGSLAEAEIVVR
                     AGKLLGGPGWLRVSYGTPEENDRFVAALGEAIKAHG"
     gene            8618..9631
                     /gene="aroF"
                     /locus_tag="PLJ64_03070"
     CDS             8618..9631
                     /gene="aroF"
                     /locus_tag="PLJ64_03070"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNC92506.1"
                     /translation="MKEGATEEEIAHVVERVEEIGCSAHISEGELLTVIGAIGDRGKV
                     ADLGLEGAPGVDHVTPISKPYKLASRETKQGADSVLEIGGRKVGGTNFALMAGPCTVE
                     NRDQMLDSADAVADAGATMLRGGAYKPRTSPYSFQGLGREGLRLLSEARERTGLPIVT
                     ELLDLRDIDDVVEVADVIQIGARNMQNYALLSEAGKSGKAVLLKRGLSASIEELLMAS
                     EYILKEGNENVMLCERGIRTYETAYRFTLDLLAVPALKEQTHLPVIVDPSHAPGRRDW
                     VESMSLAAAASGADGIIIEVHNEPEKAICDAAQQVPTSEFQELTNKLETITSVIGKTI
                     SRA"
     gene            9642..10715
                     /locus_tag="PLJ64_03075"
     CDS             9642..10715
                     /locus_tag="PLJ64_03075"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HNC92507.1"
                     /translation="MKLAVLGVGLIGGSIGLAARDRDLAEVTGWVRNPDRAEECLKVG
                     AVDHMAATVEEACADADVIVCCASVGHLIEMGRKALEAAPEGCVVTDVGSTKQELVEA
                     LGNDRRFIGGHPLAGAETAGVMNARADLYEGARWYLTPTSDSEGMLLDRLQKLVKGLG
                     AKPVAIDAVEHDRAMAVVSHLPHVFANQLAAQGRPGTERLGALAPSLRDGTRVAGSNP
                     AIWGEIFATNHEAVVAAIDESIAGLERAREVIGSADPGRIEQWQAAVGEAEAALSATE
                     TAGEATHELRVLVPNQPGVLAELALALGRAGVNITDMSLDPAPDMSSGAISLWVAGEG
                     EAEKAEECIAGLGHSVSQVVTGS"
     gene            10723..12060
                     /gene="aroA"
                     /locus_tag="PLJ64_03080"
     CDS             10723..12060
                     /gene="aroA"
                     /locus_tag="PLJ64_03080"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNC92508.1"
                     /translation="MAPVSGHEVELDGGERATFRPAGPLRGSLTPPADKSISHRAAII
                     GAIADGPTRVTNFLDSDDTRSTLSAVAGLGAMVNPEPLNAMPEEFMIEGVGPTGPRSL
                     GRIDVGNAGTLMRLLPGWLAGCDGGLWRMNGDASIRKRPVDRIATPLREMGAEIEVTD
                     GRFPPIRIDGHQLKGITYELPVASAQVKSCILLAGLSAEGPTTVIEPVATRDHTEVML
                     AAAGADIERQSGRVTIRPGAKLELDEVEVPADISSAAFFLVAATIVPGSELVIERCGV
                     NPTRTGIIDILRRMGADIELEPQAGAEGEPIARITVRSAPLTGTRIEGAEIPLAIDEL
                     PVIALAACFAEGETVIADAEELKAKESDRIAVVVGALAGFGANIEARDDGMRIIGTGG
                     LKGGKVDSHGDHRIAMLGAVAGIASREGVTVDGMDAAAVSYPTFQQDLARLQG"
     gene            complement(12144..13217)
                     /locus_tag="PLJ64_03085"
     CDS             complement(12144..13217)
                     /locus_tag="PLJ64_03085"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HNC92509.1"
                     /translation="MRVQTPGRGPLFPDQGPLGNRVKRRLIGGTAEIAGFILLTVLSP
                     LLMLVGLIVDLALWLKNRRPFVNIRLIPAVWWFLFGEMKAYLKLIYIYVTTGGPRGKG
                     SISRRRQVYQLRIAWARNHLLAIKFLFGLKFELEGMENTGPGPYVLMPRHVSILDNLI
                     GDVFIGHPQRIGVRYVIKEEIRALTPIDIGGRWIPTVFVKRGSLDPDTEIAAVRALTH
                     EMSPGEIMAIYPEGTRPTHKKIRRAQEVIAEKQPGIAPLAARLNHLLPPRLGGPLAIL
                     DEAANGIDIVFCGHVGFEGLRTVGDVMAGVLVGRQIKIRFWRFEGSTIPQGDQARTEW
                     LYEKWQMIDDWVGEELAARGETD"
     gene            13318..13956
                     /gene="cmk"
                     /locus_tag="PLJ64_03090"
     CDS             13318..13956
                     /gene="cmk"
                     /locus_tag="PLJ64_03090"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNC92510.1"
                     /translation="MVIAIDGPAGAGKSTVARGLAAELGFTYLDSGAMYRCVALTCLE
                     WGADPTASGEATSTAESIEIGFDDGDVLIDGVPVTGEIRTPEVTEAASQVSIHPGVRA
                     AMVAEQQRLIATGPYVTEGRDIGTVVAPDSPLKVFLTASPEARAERRAAETGQPVAEV
                     LAAQEERDRRDSEREHGALYAAEDAVTIDSSGMSADQVIAEIAALARDRGLA"
     gene            13956..15302
                     /gene="der"
                     /locus_tag="PLJ64_03095"
     CDS             13956..15302
                     /gene="der"
                     /locus_tag="PLJ64_03095"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNC92511.1"
                     /translation="MEAPGSSSAPSGTVPTVAIVGFPNVGKSTLVNRLAGGREAVVHS
                     EAGVTRDRKRVRCEWNGREFDLLDTGGIDLDDASEMAADIQRQARLGMAEADVVVLVV
                     NGQAGLRTGDTDLARELRTSPVPVIVAVNKSDRPDDEFAAAEFHALGLGDPTAVSATH
                     GIGTGDLLDRITEALGDRPARAEESDAVRVAVIGRPNVGKSSLVNRILGDDRVIVSPV
                     AGTTRDTIDTEIEVDGRSVILVDTAGLRRKSKVAGSVDHYAQLRSERAIERADVAIIV
                     CDAFEGVRSEDLKVGEVAMRAGCATMIVMNKWDITETDIDDTRARVGRKLRLRPEVVT
                     CSAKSGRNVAGLISRAVALADRASQRIPTPQLNRFISEVQTTTPPPSRRGRRLKLLYG
                     AQTGVSPPHFSIQVNDRRLIVRDWAYHLENRLRDQWGLDGVPLVIDYIPRKGRGDD"
     gene            complement(15362..15844)
                     /locus_tag="PLJ64_03100"
     CDS             complement(15362..15844)
                     /locus_tag="PLJ64_03100"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNC92512.1"
                     /translation="MAMVRWEPSRELEAFQTDMNRLFDSFFRSGSNSTVRRWAPATDL
                     TEDGNDLVLRLDLPGLSDEDVAVEVEGNVLTVSGERRDERETKEGSAYRLERSFGKFS
                     RSFTLPEGTDPDAIEGGIDKGVLELRIPKPTEKQPRRVELGASREEARAAIEGTSSEA
                     "
     gene            16064..17293
                     /gene="metK"
                     /locus_tag="PLJ64_03105"
     CDS             16064..17293
                     /gene="metK"
                     /locus_tag="PLJ64_03105"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNC92513.1"
                     /translation="MTDLKLAEGEHLFTSESVTEGHPDKIADQISDTILDACLSQDPH
                     SRVACETLVNTGMAVISGELTTSAQIDITDLVRKTVCEIGYDKGEYGYDGNNISVLVS
                     MDKQSPDIAQGVDTGIETRTGGSSDQFDLDGAGDQGMMFGYATNETEALMPMPIQIAH
                     RLSERLSLVRKDGTLPYLRPDGKTQVTVRYADGKPIAVERVLISTQHAPDVDNENTIR
                     PDLWENVILPVLAEEYGDLLDENALYESLLINPTGIFVIGGPVGDAGLTGRKIIVDTY
                     GGAARHGGGAFSGKDPSKVDRSAAYAARYAAKNVVASGLADRCEVQVAYAIGVARPVS
                     IMAETYGTGKKSDWEISQLVADNFDLRPGAFRQYLDLHRPIYKDTAAYGHFGRSNENF
                     TWERTDRAESLKEAAGL"
BASE COUNT         3015 a         5735 c         5801 g         2839 t
ORIGIN      
        1 gcagcagctg gaaatccgac ccaccggacc atgcgaaccg ggggttggac accgcagcgg
       61 gtcgtaaaac cggtcctcgg gccgaaaatc cgacccacga gacctgcgaa tcagttccgg
      121 aggtgccagt gggtcggatt ttcggggcgg gtgacggaaa tccgacccac tgggggatcg
      181 ggtggggttg gaatcaccga atgcccactt cgtgccggta tgtggcgcaa agtgggatct
      241 cgcggtgctc gggtcgggct ggtcgtggag gtgtcgcacg aacaggccca tcacggtgct
      301 ggatttgcgg cacctccggg cctgacgggc tggtgtggcg ttcaggcggg gattttgggg
      361 aggtcctgcc ggcggcggcc ggggccgggt ttcatgtaga cccggtccgg ctcgactgat
      421 tcgccgcact tttcgcagac catgtggtgg tcgaggttgc cgccgcaggt gcggtggacg
      481 gcgatggtcg gagggccgcc gggctcgggg tagtactcgt caccccacca catcagctgc
      541 agcaggacgg gggagaggtc gcggcccttt tgggtcaggt ggtattcgaa gcgcgggggc
      601 ttctcgctgt actggcgctt ctcgaggatc ccgtattcga tcagggtgtt gaggcgcgtg
      661 gtcaggacgc tgcgagtgat cccgaggttc tcgcggagcg gctcgaaacg gttcaggccg
      721 aggccgacgt tcctgatgat caggagtgac cagcgctcac cgaccacctc gagcgaacgg
      781 gcgatcgaac agacctggtt gtcgtactcg cgatcgagca tggaccgatt gtagcgggtc
      841 agtcgcatca tgcgacccga ttgtgttagg gtcagtcgca tgaagatact cactcgggac
      901 aagtgaacat gtcggacacc gtgaccggaa agttcgaggc agtggaagag gaaatcctgg
      961 tcggtgctcc gaccccggcc gcaggcccgg tgccgaagga gatggcggag gcggaagcgg
     1021 cctaccggcc cgaggtcctc ggcaaggtgc tggcggtcgt ggcagcggcg gtggccctgg
     1081 ccagcctcga catgttcatc gtcaacatcg ccttcccggc catcgaagcc gactttcccg
     1141 gcagttcgat cggctcgctt tcctgggtcc tcaacgggta cgtgatcgtc ttcgccgctt
     1201 tgctcatgcc ctttggccgg ctggccgacc gggtcgggcg caagcgaatc ttcacgaccg
     1261 gggtgttgat cttctgtctt gcttccggag cctgcgccgc ggcctggtcg gtcgagaccc
     1321 tggtcgcctt ccgggtcatt caggggatcg gcgcggcgat gctgatgtcc acttcgctgg
     1381 ccctgctgct ccacgccttt ccggcggagc gctggccggt cgtgatcggc gtctgggcgg
     1441 cggcgggcgg cgcggccggc gctctcggtc cgccgatcgg tggtttgctg gtcgaaatct
     1501 cctggcgatg ggtcttcctc gtgaacctgc cgctttcgct cgcagcctgg tatttcggga
     1561 cgcggctgct ggtcgagtcg aaggatccac gcgaaaccag gtggcccgac ccggccggca
     1621 tggccctgat gatcctcggc atcggggtgc tctgctgggg actggtcgaa gcgccggacc
     1681 atggttgggg cagcatccgg accgtcgtcg cgatcctcgc cggcctggtc atgctgggac
     1741 tgacctttct gcgctcccga cggccggccg atcgctggat cccgaccctg gacgtggatc
     1801 tcttcaaggc caggcccttc gcgatggcct gtctgaccgc gctgatcttc atggtggcct
     1861 tcgccgcgat actcctcggc ggggtcctgt tcctgaccac tgtttggggt ttctccgtgc
     1921 tggaagcggg gatcgccttc ttcccggggc cggctatggc cgggatcttc tcggttctcg
     1981 gcagccggtt cggagcccgg ttcggcaccg ggccggtggc cgcaaccggc tgcgggttcc
     2041 tgatcctcgg gggaacgtgg tggttcaccc ggatggggga tacatccgcc tacttcaccg
     2101 agaacctgcc cggacagatc ctggtcggca tcggggtcgg actcgtcctg ccgaacgtcg
     2161 cagcagctgt ggcttcaacc ctgccgccaa cctcgctggc gaccgggacg gcagttctga
     2221 gcgcttcccg tcaggtcggc gccgcgctgg gtgtggccat cctcgtcgcc ctgctcggtt
     2281 cggccgcggt cgtcggcacc gccgatgatt tcgacaaggc ctggctgatg atcgcgatct
     2341 ccgccgcaat cgccggtgcc accgccctga ccataggacg cgccggcaag accgaataaa
     2401 tagtctgcgt cccgatgcga atcttttcgg gaatccagcc gaccgggcgc aagcacctcg
     2461 gcaactacat cggggcgatc aggcagtacg tcgagggtca ggaccgcggc gacccggcga
     2521 tctactcgat cgtcgacctg cacgcgatca ccgtcgccta tgagcccggg accctgagaa
     2581 ccaacctcta cgacacgacc gccgcgattc tcgccgccgg ggtcgacccg gagcgctgca
     2641 tcctcttccg ccagtccgac atccccgagc acaccgaact ctgctggctg ctctccggag
     2701 tctgtgccca cggcgacctc aaccggatga cccagttcaa ggagaagtcg gcccagcaga
     2761 aggatctggt ttcggccggg atcttcatct acccgctgct tcaggccgcc gacgtactcg
     2821 cctaccaggc agacgaggta ccggtcggcg aggaccagcg ccagcacatt gaactgatgc
     2881 gtgaggtcgc ccggcgtttc aacgaacgct tcggcgaaac cttcacgatt cccgagcacc
     2941 ggatcccgga gatcggggcg cgggtaatgg acctgcagga gccaggccgc aagatgtcga
     3001 cctcggtcgg cttcgacagc ggtcgcgtct acctgctcga aaccgagaag gaaacgcgca
     3061 agaagctcgg ctccgcggtg accgactccg gtcgcgaggt gaagcgcggt gaaggcaagg
     3121 aaggggtcgc caacctgatc ggcatctacg cggtggccac cggcaagacc gaagacgaag
     3181 tcgaagccga attcgagggc gccggttacg gcgacttcaa gaaggccgtg gccgatgcgg
     3241 tcgtcgagtt cctcgctccg gtccgggagg cctacccggc gatccgcgaa gacgagggcg
     3301 agatcgaacg gatcctcgcc gacggggcgg ccaaggcccg ggaaatcgct tcggtgaccc
     3361 tggcggacgt gcgcgaggca atgggcgtcg gcgtgccgcg cggatagcgc gaacgaaggc
     3421 atcgaagatc cgcaaatagc gcctttccgg agcggggccg accgatcgcc cctctaccct
     3481 ttttgcgatg gcttcccggc tggacctgga cctcgatctg gatctcgatg tcttcatcgg
     3541 gcccttcgac cttctcctca gcatcgtgct gcgggaggaa atcagcctgc ttgaagtcga
     3601 actcggcgag atcgtcgcca tgtacgtcgg gaagctcgag gaagagggcg agctcgaact
     3661 tgaagtagcg accgagttcc tggttctcat cgccgccttg ctggagttga agtcgcggct
     3721 gatgctgccg ggggccgatg aggacctgac cgacatggag ccggcagagg cggtcgagga
     3781 actggtcgcc cggatgctgg aataccggcg ctaccgggac gccggccggg ccctcaagga
     3841 gcacttcgaa agccagcggg ggttcctcta ccggtcggcc ccgctgccgc cggagctgcg
     3901 tcgggtcgag gtcgagaccg cggagaaggt ctatgaaccg gagcagctgg cggccgcgct
     3961 cggcgatctg ctcgaaccgc cgccccggcc ggacctctcg cacctgcgcg cgaccgtgtc
     4021 tctgcgccgt cgcctttcgg tcgtccggtc ggtcctgatg aaccggccgg tcttcgattt
     4081 cgatgacgag ttcggcaacg aggaccggat gacccaggcg gtaacgatct tcgccctgct
     4141 cgacttgtac aagaagggcg aagcggtctg ggaacagacc gaatcctgcg gaccgatcac
     4201 ggttaaacgg attcaggccg gacaaaacgg attggcgagg actgcatgac cgatcttgcc
     4261 cgacggatcg aatcgctgct gttcctcagc aaccagccgg tgaccgagat cgccctggcg
     4321 gaagcctgcg aagtggagct cgagcaggtc cagaacgcgg tggtcgaact cgaagaggac
     4381 ctggagcccg gacggcgcgg gctgatcctg cggcgggtcg ggggagggct gatcctggcc
     4441 agtgatcccg agtcggaagc ggccgcccgc aggctgctct cgaagccccg caccccgccg
     4501 ctcacccagg cccaggccga gacgcttgcg atcgtcgcct acctgcagcc ggttgcccgg
     4561 cctgagatcg caaggatccg cggcgtttcc tccgagtcgg cggtcaacag cctcagcgag
     4621 cgcggcctga tcgaggaatc cggtcgctcc cgcttcggcg ccgcgatcta ccggacctcg
     4681 cccctgttcg agaaggtctt cggactcgag ggcctcgacg cgctgccgga tccgacccgg
     4741 ttcgatccga ccccggaaga cgaacgcgag attcgcgagc gcctgcttgc cgcgggcgaa
     4801 cagcgctctg aagcctgacc ggctgcccgc ggtccttccg gcgcgaccgg taggtttctg
     4861 ggtgttcggt aacgcccagt cctaagctag ggagaccagt cctttgaaga agttcgcagt
     4921 cctgcttgcc ttgctcgcat ccgtttttgc cgtcgccgcc tgtggcagca acaacaaccc
     4981 gaccgtcacg ccggacacgc tcgcctcggc gatggacgag gtctgcaccg acaccaacgc
     5041 cgacttcgaa gcgatgggca cccgcggcct gaccatggac gggatcgcgg ccgagttcga
     5101 gggctacgcc gaggtgcggc aggcagtcgt cgacgggttc aacgagttga acaccgacga
     5161 ccaggccgag ccgcagctcc agaagtacat cgacgcctcc gagcaggtca tcgcccagga
     5221 caaggcgatc gccaaggccg ccgccgaagg cgaccagaag gccatgaacg aggctttcgc
     5281 cgcccagacc aaggcattca acgagcgcga caaggtggcg aaggagatcg gcaccgaggt
     5341 ctgcggtcag acacccgaga tcaaggttga aaagaccggg accgcgccgc cttccgacct
     5401 caatgtcgtc gagccgaaga acacggtcga ggatgccgcc gacaactaca tcgaggcgac
     5461 cgcgtccggc aactgcggcc tgatcaacgc caaccggcac accgacgccg gtgacctcga
     5521 tgaagccagc tgcaaggcag ccggccaggc tctgaagggc gccagggtga tcggcaccga
     5581 gagttacggc cccgtggccc aggccgagat cctcagcgga aacggcacgc actatccgac
     5641 ctacttcgtc accgaccttg acggcaacct ccggtatgga ggtgacgcga tccacgacag
     5701 cggcggactg cggccggccc cggaaggaaa cgacgccgag gcgacagtcg aggcgaccgt
     5761 caacgcgatt cgcgacaacg atgccgccgc cttcaacgag accctggcct tcgaggacag
     5821 cccgttctgg atcaagggcg acactgtgga gaagttcggt gagggtgcct acaacgagga
     5881 attcatcaag gacgtcaagg acggggacgc cgacccgatt cagctcgggc tgaactcgac
     5941 ctgggctttc tacttcctgg aaggcagcaa gtacgactgg gtcctttcgt ttacccacaa
     6001 ccccggcatc ggtggccatt acgccttcac cggctactac ccgattccca agccgaactg
     6061 atccggctga aatagttccg gccatgcggc tggccaagta cctcgcccat gcaggcgtcg
     6121 cctcgagaag gaaatccgag gagatagtcg ccgctggccg ggttcgggtg gccggaaagg
     6181 tgatcaccga cccggccttc ggggtcgagg gtggcgagga ggtccgggtt gacggccatc
     6241 aggtcgagcc cgagggccgt gaggtctgga tgctcaacaa gccgctcgac gtgatctcca
     6301 ccgccgacga gccgggcaag cggatcgccg tcgtcgacct ggtcgacagc aacgcccggc
     6361 tctacccggt cggccggctc gacgccgact cgaccgggct gatcctgctc agcaacgacg
     6421 gcgagctcgc caaccggctc acccacccgc gcttcgaggt cgagaagacc taccgggtga
     6481 agctgaagaa gccggtcacc ccggaagcga tcgatcagct ccagcgcgga gtccgcctgg
     6541 atgacggcat gaccgcgccc gccaccgtga agaagctggg cgaggccgaa ctggacgtca
     6601 cgatccacga aggccgcaac cggcaagtga gacggatggc cgaagcgatc ggcaacgaag
     6661 tggtcgctct gcagcggatt tcgctcgggt cgctgaagct cgggcgactc gggatcggca
     6721 agtgcaggtt gctcgacgaa cgcgaggtgg agcggctctg gaaagatgca ggcgatgact
     6781 gatccagagg cagaaggcat gcgggtcgcg gcgatccgcg gcgcggtcca ggcggagaac
     6841 gacgccgacg tgatcctgtc cgcgaccgag gagctgatca gggagatcat ctccgtcaac
     6901 gacctcgagc cggaccggat gatcagcgcg atcttcacca ccaccgagga cctcgacgcg
     6961 gagttcccgg cggcggcagc ccgtcgcatg ggtctgaacg aggtgccgct tatgtgcgcc
     7021 cgcgaaattc cggtgccggg cgcgatgccc ggggtgatcc gggtcatgct ccacacctac
     7081 ctgccggccg gggcgaaggc ccgtcacgtc tacctcggcg agacccgcaa cctgcgggcc
     7141 gacctcgacg cggcccagta gcagcccgcc ctcaacccac ggatacacga tgaccgttcg
     7201 tttcaactcc cacctgactt cgatccccgg ctacagcccc ggagtcccga agggccacag
     7261 cgccgaagac gtcgccagct ccaatctggc ccagctcgct tccaacgagt caccgttcgc
     7321 cccgctgccc gaagtggtgg aggcgatcac cagggcggcc aaagcgatga accggtaccc
     7381 ggacccggcc gcgaccaggc tgcgccagcg actggccgac cggcacgaga tcgagcccgg
     7441 acaggtatcg gtcgccaacg gctcctgcga attcctgctc gccgccgccg aggcgctgct
     7501 cgagcccggg gcggagatcg tgtacgcctg gccctcgttt tcgatgtatc cgaacatggc
     7561 cgcgatgacc ggtgcccggg aaatccgcgt tcccctgaag gaagaccaga cccacgacct
     7621 cgaagccatg ctggcggaga tcaccgccgc gacccagatc gtgatcgtct gcaatccgaa
     7681 caatccgacc gggacctaca ttccgttcgc cgagatcgcc gacttcgtcg aacgggtgcc
     7741 cgaccacgtc cagatcatcc tcgacgaggc ctacatcgag ttccaggtct acgacgatcc
     7801 ggccaactcg atcgacctgc tgaagcggca ccccaacctg gtcctgctcc gcactttcag
     7861 caagacccac tcgctggccg gcctccgggt cggttacgcg ctcggctcac ccgccttcaa
     7921 ggcggcagtg gaagcggtgc gccagccctt cagcgtcaac gcgctggccc aggccgccgc
     7981 ggtcgaggcg ctcgagcacg gcgacgtgat ccagcggcag atcgaaaccc agatcgccga
     8041 gcgggtgcgg gtcgagtccg gggtggccga cctcggcctg cgggcgaccg acagccaggc
     8101 caacttctcc tggatctcgc ttggcgataa cggtgcggag atcgaagatg cggtggtcgg
     8161 ttcgctggcc gaggcggaga tcgtggtccg ggccggcaaa ctgctcggcg gccccggctg
     8221 gctgcgggtt tcctacggaa cgccggaaga aaacgaccgt ttcgtggccg cgctcggcga
     8281 agcgatcaag gcgcacggct agcgccccgc gcggccgatc gttggcgcta agccgggcga
     8341 cctgatacaa actggcccaa tgccgcagca gaccgacagc cgcatcgccc cttcgggcac
     8401 ggccctccgc aagagcggaa cccccggtgc tgccttttat tcctattggc gatttatcta
     8461 ggcgtccgtc cggtgatgcg ggtagctgcc cgccagtgaa ttcacagccg ccgggcgccg
     8521 tgtcattcgg tgtcgtccgc tgccttcggc cggacgaacg caggtaaggt ctttggcagt
     8581 acaggaaagg tagaagcaaa aaacaatgat gatcgtgatg aaagaggggg cgaccgaaga
     8641 agagatcgcc catgtcgttg aacgggtcga ggagatcggc tgttcggcgc acatttccga
     8701 gggagaattg ctgaccgtca tcggggcgat cggggatcgc ggcaaggtcg ccgatctcgg
     8761 tctggaagga gcacccgggg tcgaccacgt gactccgatc tcgaagccct acaagctggc
     8821 gtcccgagaa acgaagcagg gtgccgactc ggttctcgag atcggcggcc gaaaagtcgg
     8881 gggaacgaac ttcgccctga tggcgggtcc ttgcacggtt gaaaaccgag accagatgct
     8941 ggactcggcc gacgccgtgg ccgatgccgg cgcgacgatg ctgcgaggcg gcgcctacaa
     9001 gccccggacc tctccgtaca gcttccaggg cctcggccgc gaagggctgc gcctgctttc
     9061 cgaagcgcgc gagcgtaccg gcctgccaat cgtgaccgag ctgctcgacc tgcgcgacat
     9121 cgacgacgtg gtcgaagtgg cggacgtgat ccagatcggc gcccgcaaca tgcagaacta
     9181 cgcgctgctt tccgaagcgg gcaagtcggg caaggccgtg ctgctgaagc gcgggctttc
     9241 ggcctcgatc gaggagcttc tgatggcctc cgagtacatc ctcaaggaag gcaacgagaa
     9301 cgtgatgctc tgcgaaaggg gcatccggac ctacgagacc gcctaccgct tcacgctcga
     9361 cctgctggca gtgcctgccc tgaaggagca gacgcacctg ccggtgatcg tcgatccgag
     9421 ccacgcaccg ggtcgtcgcg actgggtcga gtcgatgtcc cttgccgctg ccgcttccgg
     9481 tgcggacggg atcatcatcg aggttcacaa cgagcccgag aaggcgatct gcgatgcggc
     9541 ccagcaggtt ccgacctccg aattccagga gctgacgaac aagctggaga cgatcacctc
     9601 ggtgatcggc aagacgatct cgcgggccta gagggggttc gatgaaactg gccgtcctcg
     9661 gagtcgggct gatcggaggc tcgatcggtc tcgcggcacg ggaccgggat ctggccgaag
     9721 tcaccggctg ggtgcggaac ccggaccggg cggaggaatg cctcaaggtc ggggcggtcg
     9781 atcacatggc ggccacggtc gaggaggcct gcgccgacgc ggacgtgatc gtctgctgcg
     9841 cctcggtcgg gcatctgatc gagatgggcc gcaaggctct cgaggctgcc cccgaaggct
     9901 gcgtggtgac cgatgtcggt tcgaccaagc aggaactggt cgaagcgctc ggaaacgacc
     9961 ggagattcat cgggggtcac ccgctggccg gcgccgagac ggccggggtg atgaacgccc
    10021 gtgccgatct ctacgagggt gcccgctggt acctgacccc gacctcggac tccgagggga
    10081 tgctgcttga ccggctgcag aagctggtca agggcctcgg cgcgaaaccg gtcgcgatcg
    10141 acgccgtcga gcacgaccgg gcgatggcgg tggtcagcca cctgccacat gtatttgcca
    10201 atcagctcgc cgcccagggt cggccgggca ccgagcggct cggcgccctc gctccgagcc
    10261 tccgtgacgg cacccgcgtc gccggctcga accccgcgat ctggggcgag atcttcgcca
    10321 ccaatcacga ggcggtggtc gccgcgatcg acgagtcgat cgccggactc gagcgcgcca
    10381 gggaagtgat cggctcggcc gaccccgggc ggatcgagca gtggcaggcc gccgtcggcg
    10441 aggccgaggc ggcgctttcg gcaaccgaga ccgccggtga agcgacccac gagctgcggg
    10501 tgctggtccc gaatcagccc ggcgtgctgg ccgaactggc cctcgccctg ggccgggccg
    10561 gggtcaacat caccgacatg tcgctcgacc ctgctcccga catgagctcg ggtgcgatct
    10621 cgctctgggt cgccggggag ggcgaggccg agaaggccga agaatgcatc gccgggctcg
    10681 gccactcggt cagccaggta gtgaccggat catgatccgg cggtggctcc ggtgagcggt
    10741 cacgaagtcg aactcgacgg cggcgagcgg gccaccttcc ggcctgccgg gccgctgcgc
    10801 ggctcgctga ccccgccggc cgacaagtcg atctcccacc gggcggcgat catcggagcg
    10861 attgccgacg gcccgacccg ggtcaccaac ttcctcgact cggacgacac ccgctcgacc
    10921 ctttccgccg tggcgggact gggggcgatg gtcaacccgg agccgctcaa cgcgatgccg
    10981 gaggagttca tgatcgaagg ggtcggcccg accgggcctc gcagtctggg gcgaatcgac
    11041 gtcggcaacg ccggcaccct gatgcgcctc ctgcccggct ggctggccgg ctgcgacggc
    11101 gggctctggc ggatgaacgg cgatgcctcg atccggaagc gcccggttga ccggatcgcc
    11161 accccgcttc gcgagatggg ggcggaaatc gaagtgaccg acggtcgttt cccgccgatc
    11221 cggatcgatg gccaccagct gaagggcatc acctacgaat tgccggtcgc ttcggcccag
    11281 gtcaagtcct gcattctgct ggccgggctg agcgccgaag gcccgaccac cgtgatcgag
    11341 ccggtcgcga cccgcgacca caccgaagtg atgctggccg cggccggcgc cgacatcgaa
    11401 cgccagtccg gccgggtcac gatccggcca ggcgcaaaac tcgagctgga cgaggtcgag
    11461 gtcccggccg acatttcttc cgccgccttc ttcctggtcg ccgccacaat cgtccccggc
    11521 tcggagttgg tgatcgagcg ctgcggggtc aacccgaccc gcaccggcat catcgacatc
    11581 ctccgccgga tgggcgccga catcgagctc gagccgcagg ccggggcgga gggcgagccg
    11641 atcgcccgga tcacggtccg gtcggccccg ctgaccggca cccggatcga aggggcggag
    11701 atccccctgg cgatcgacga gctgccggtg atcgccctcg cggcctgttt cgccgagggg
    11761 gagaccgtga tcgccgacgc cgaggagctc aaggcgaagg agtcggaccg gatcgcggta
    11821 gtggtgggtg cgctggccgg tttcggggcg aacatcgagg cccgcgacga tggcatgcgg
    11881 atcatcggca ccggtggcct gaagggcggc aaggtcgatt cccacggcga ccaccgcatc
    11941 gccatgctcg gcgcggtcgc cggcatcgcc agccgggaag gcgtgacggt cgacggcatg
    12001 gatgcggcag cggtcagcta cccgaccttc cagcaggatc tcgcccgcct tcagggctag
    12061 cgcccccggt cgcgagatgc gtctggtcgg cgaataccgc cgaccaaacg cacctcaccg
    12121 cgctcgacag tcctgaattt gcctcagtcg gtttcgccgc gggcggccag ttcctcgccg
    12181 acccagtcgt cgatcatctg ccacttctcg tagagccact cggtgcgagc ctgatcgccc
    12241 tgggggatgg tgctcccctc gaagcgccaa aagcggatct tgatctggcg gccaaccagc
    12301 accccggcca tcacgtcgcc gaccgtgcgc aggccttcga agccgacgtg gccgcagaag
    12361 acgatgtcga tgccgttggc ggcctcgtcg aggatcgcca gcggcccacc caggcgcgga
    12421 ggcaggaggt ggttgagccg ggccgccagc ggggcgatcc cgggttgctt ctcggcgatc
    12481 acttcctggg cccgcctgat cttcttgtgg gtagggcggg tcccctcggg atagatcgcc
    12541 atgatctcgc ccgggctcat ctcatgggtc agcgccctca ctgcggcgat ctcggtgtcg
    12601 gggtcaagcg agccgcgctt gacgaacacg gtcgggatcc agcggccgcc gatgtcgatc
    12661 ggggtcagcg cccggatctc ctccttgatc acgtagcgga ccccgattct ttgcgggtga
    12721 ccgatgaaga cgtccccgat caggttgtcg aggatgctga cgtgccgggg catcaggaca
    12781 tatggccccg gtccggtgtt ctccatgcct tcgagctcga acttcaggcc gaacaggaac
    12841 ttgatcgcga gcaggtggtt gcgggcccag gcgatcctta gctggtagac ctgccggcgc
    12901 cgggaaatcg accccttgcc ccgggggccg ccggtcgtga cgtagatgta gatcagcttc
    12961 aggtaggcct tcatctcccc gaacaggaac caccagacgg cggggatcag gcggatgttc
    13021 acgaaggggc ggcggttctt cagccagaga gcgaggtcga cgatgaggcc gaccagcatc
    13081 agaagcggcg agagcacggt cagcaggatg aatcccgcga tttcggcggt gccgccgatc
    13141 aggcgccgtt tgacgcggtt gccgagcggt ccctggtcgg gaaagagtgg gccccggccg
    13201 ggggtctgga cgcgcatcgg ctgatcgtat tcgggccggt gctctttccg cgccccgccg
    13261 ggttacccgc aggtctcccg gcccgtagga tgggcggtcg atgaaaggca gattcacatg
    13321 gtgatcgcga tcgacggtcc ggccggggcc ggaaagtcaa ccgttgcccg cggccttgcc
    13381 gcggagcttg gtttcaccta cctcgattcc ggggcgatgt accgctgcgt ggccctgacc
    13441 tgtctcgaat ggggtgccga cccgaccgcc tcgggggagg caacttccac cgcggagtcg
    13501 atcgaaatcg gcttcgatga tggcgacgtg ctgatcgacg gcgtgccggt gaccggggag
    13561 atccgaactc cggaggtcac cgaggcggcc tcccaggttt cgatccatcc cggtgtgcgg
    13621 gcggcgatgg tggccgaaca gcaacggctg atcgcgaccg ggccgtacgt gaccgaaggc
    13681 cgcgacatcg gcaccgtggt cgcccccgat tccccgctga aggtctttct caccgcctcc
    13741 ccggaagcga gggccgagcg ccgggcggcc gagaccggcc agcctgtggc cgaggtgctg
    13801 gccgcccagg aggaacgcga ccggcgtgac tcggaacgtg agcacggagc cctctatgcc
    13861 gccgaagacg cggtgacgat cgacagcagc ggcatgtccg ccgaccaggt gatcgccgag
    13921 atcgccgctc ttgcccgcga ccggggcctg gcctgatgga agcgccaggc agttcctcgg
    13981 ccccgtcggg gacggtgccg accgtcgcca tcgtcggttt ccccaatgtc ggcaagtcaa
    14041 ccctcgtaaa ccggctggct ggcggccgcg aggcggtcgt tcactctgaa gccggggtca
    14101 cccgggatcg caagcgggtt cgctgcgagt ggaacggccg cgaattcgac ctgctcgaca
    14161 cgggtggcat tgacctcgat gacgcgtcgg agatggcggc ggacattcag cgccaggccc
    14221 ggctcggcat ggccgaggcc gatgtcgtcg tgctggtcgt caacggccag gccggtctgc
    14281 gcaccggtga cacggacctt gcccgcgagc tacgcacctc gccggtaccg gtgatcgtgg
    14341 cggtcaacaa gtcggatcgg ccggacgacg aattcgccgc ggcggaattt cacgcgctcg
    14401 gccttggtga tccgaccgcg gtctcggcca cccacgggat cggtaccggt gatctgctcg
    14461 accggatcac cgaggctctc ggagaccgtc ccgcccgggc ggaggaatct gacgcggtga
    14521 gggttgcggt gatcggccgg cccaacgtcg gcaagtcctc gctggtcaac cggatcctcg
    14581 gcgacgaccg ggtgatcgtt tccccggttg ccggcaccac ccgggacacg atcgacaccg
    14641 agatcgaggt tgacggccgc agcgtgattc tggtcgacac cgcggggctt cgtcgcaagt
    14701 cgaaggtcgc cggttcggtc gatcactacg cccagcttcg ctccgagcgg gcgatcgaaa
    14761 gggccgatgt ggcgatcatc gtctgtgacg ccttcgaagg cgtgcgctcc gaggacctga
    14821 aggtggggga ggtggcgatg cgggccggct gcgccacgat gatcgtgatg aacaaatggg
    14881 acatcaccga gaccgacatc gatgacaccc gggcccgggt cgggcgaaag ctccgcctgc
    14941 gccccgaggt tgtgacctgt tcggccaagt cgggccgcaa cgtcgccggc ctgatctccc
    15001 gtgcggtcgc gctggccgac cgggcctccc agcggatccc gaccccgcaa ctcaaccgct
    15061 tcatttccga agtccagacc acgaccccgc cgccttcccg tcgcgggcgg cgtttgaagc
    15121 tcctctacgg agcccagacc ggcgtgtcgc cgccgcattt ctcgatccag gtcaacgacc
    15181 ggcgattgat cgtgcgcgac tgggcctacc acctcgagaa ccgccttcgc gaccagtggg
    15241 gcctcgacgg agtcccgctg gtgatcgact acatcccccg aaaaggccgc ggggacgact
    15301 gaagacacga aaaaggggtc accggcgtga cccctttttc ggtcctgttc ttcacctcgg
    15361 gtcaggcttc agacgaggtg ccttcaatcg ctgcccgtgc ttcttccctg gaagcgccca
    15421 gttcgactcg gcggggctgc ttctcggttg gcttggggat gcgcagttcc agcacccctt
    15481 tgtcgatccc gccctcgatc gcgtcgggat cggttccctc cgggagggtg aaggagcgac
    15541 tgaacttccc gaacgagcgt tccagccggt aggcgctgcc ttccttggtc tcccgctcgt
    15601 cccggcgttc ccccgagacg gtgagcacgt tgccctcgac ttccaccgcc acgtcctcgt
    15661 cggacagtcc tggcagatcg agccgtagaa ccagatcgtt tccgtcctcg gtcaggtcgg
    15721 tggcgggcgc ccacctccgc acggtcgaat tggaacccga gcgaaagaag ctgtcgaata
    15781 accggttcat atcggtctga aaagcttcca gttcccggga tggttcccat ctgaccattg
    15841 ccattcgctt tctcctttct ggtctttcga ggactcggaa tagtccccga ccaattcgag
    15901 ggccatcata aaatctaagt gccgacatgt caagtctgag agacgcacat tttccggtcg
    15961 aagtagcgcg ggcattctgg atcgaggttt catctaggct tcgcaaccac atgcactcgc
    16021 tgagcgaggc ttctgtgcca gaccccaagg agaaacttcc cgaatgaccg atctgaagct
    16081 tgccgagggt gaacacctct tcacctcaga atccgtaacc gaaggccatc ccgacaagat
    16141 cgccgaccag atctcggaca cgattctcga cgcatgcctc agtcaggatc cgcattcccg
    16201 ggttgcctgc gagaccctgg tcaacaccgg catggccgtg atctccggcg agctgacgac
    16261 ctccgcccag atcgacatca ccgacctggt ccgcaagacg gtctgcgaga tcggttacga
    16321 caagggcgag tacggctatg acggcaacaa catctcggtg ctggtctcga tggacaagca
    16381 gtccccggac atcgcccagg gcgtggacac ggggatcgaa acccgtaccg gcggttcttc
    16441 ggatcagttc gacctcgacg gggccggcga ccagggaatg atgttcggtt acgccaccaa
    16501 cgaaaccgaa gccctgatgc cgatgccgat ccagattgcc caccggcttt ccgagcggct
    16561 ctcgctggtc cgcaaggacg gcacgcttcc gtacctgcgg cccgacggca agacccaggt
    16621 caccgttcgt tacgccgatg gcaagccgat tgccgtcgaa cgggtcctga tttcaaccca
    16681 gcatgccccg gacgtcgaca acgagaacac gatccgtccc gacctctggg aaaacgtaat
    16741 cctgccggtc ctcgccgagg agtacggcga cctgctcgac gagaacgccc tctacgagtc
    16801 gctgctgatc aaccccaccg ggatcttcgt gatcggcggc ccggtcggtg acgccggcct
    16861 gaccggccgc aagatcatcg tcgacaccta tggcggcgcc gcccgtcacg gcggtggcgc
    16921 cttctcgggc aaggatccgt cgaaggtgga ccgttcggcc gcctacgcag cccgttacgc
    16981 cgccaagaac gtggttgcct ccggcctcgc cgaccgctgc gaggtccagg tcgcctacgc
    17041 gatcggcgtc gcccgcccgg tttcgatcat ggccgagacc tacggcaccg gcaagaagag
    17101 cgactgggag atttcccagc tggtcgccga caacttcgat ctccggcccg gtgccttccg
    17161 ccagtacctc gacctccacc gcccgatcta caaggacacc gccgcctacg gccacttcgg
    17221 ccgctcaaac gagaacttca cctgggagcg caccgacagg gccgagtccc tgaaggaagc
    17281 cgccggcctt taggcgattc acggcacgtt tcgaatcgag tggcagaagt cagtcagatc
    17341 cgctgacttc tgccatctcg atcttgagcg ggtcgaaccc gccccaattg
//
LOCUS       DAOQKN010000026        17176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_1505_length_17176_cov_6.009345, whole genome shotgun sequence.
ACCESSION   DAOQKN010000026 DAOQKN010000000
VERSION     DAOQKN010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 17176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17176
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1505_length_17176_cov_6.009345"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2024)
                     /locus_tag="PLJ64_03110"
     CDS             complement(<1..2024)
                     /locus_tag="PLJ64_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92514.1"
                     /translation="MAFRNRFAALAVILLAFTLFAISAVAATDGAAAARYVVAQCGWH
                     VGQDASWFDSSADRFGKSTYCQTPDSADPFEGVHLISQVKSSVDSVGGTKFASWRWQA
                     PNGTGIVNVHGQRWQYLREGFQHRLGGVPQNGSFTPFLELDYSDGTKRDFWQGFEPYA
                     QAFESRLVCYRTSDRNCEADGTVLAGVRSLTISIDDAVRPTAQISGGLAATDWLRGTQ
                     SLTFSNRDVGAGLRYAQTSIDGALRASTEMNCAKTMISGQWRGTKMQPCPTAATDSQT
                     VDTRTLADGPHSLHHCAFDFAGSYGCTADRTIRVDNNPPAAPKGLTVEGGEVWQRTNS
                     FNLEWTEPDQGPASPIVLSYFRLTGPDGYAGGPWYRTDPGKISGITLPGPGEYKARVW
                     LADQAGNSDEAHAAEATLRLDNVPPTGYFVDPPEDDPALIRVSVADRFSGVQGGSVAW
                     RPADGGDWHPISSDYLGDRQMLEARFPDDLPRGSWVLRAAIADGAGNLTVTDRRANGS
                     QMIVRTPLLDETQVSASLGSSRASRGRGTVEIGYGERAHLEGRLTGVESGGLGGATLT
                     VTETPFPGSRAKVTSRQVRTDARGYFDLWLARGPGRRVVVGFAGTRRLQGSDSGVLEL
                     RVRGRLGFKARPKRLRTGRRVYFTGRVAARGAWQPTRGNLIQIQYFEESAR"
     gene            2157..3317
                     /locus_tag="PLJ64_03115"
     CDS             2157..3317
                     /locus_tag="PLJ64_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HNC92515.1"
                     /translation="MSLPTEEQVKEKLTAVIDPELRRNIVELGMVRSVEIKDDGQINV
                     TVSLTTPGCPIRSHFQEAVVQQVSSLDGVTGVGVGFDVLSDEEKSGLRETLGRGKLPE
                     GALAQVKNVICVASGKGGVGKSTMTANLAAALQAEGKKAGALDCDVYGYSIPRMLGVD
                     QKPEVNSDRKILPPVAESGVKTMSIGYFVEQNSAVVWRGPMLHKAIQQFLEDVAWGEL
                     DYLLLDLPPGTGDIAMTLAQLLPQAKITIVTTPQPAAQSVARRSAEMAAKVDLEVMGI
                     IENMAGFTTPEGETFSIFGEGGGQLLADELEVPLFGSVPLSEELREHADVGNPLSVDQ
                     PDSPAGQAIVEIARKIIATSPIELPMIETIQTTAPEIAPKMGKNARELPLVQ"
     gene            complement(3451..3714)
                     /locus_tag="PLJ64_03120"
     CDS             complement(3451..3714)
                     /locus_tag="PLJ64_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HNC92516.1"
                     /translation="MSYVIAEPCIGQKDNSCVEVCPVDCIHPTPDEPGYDAVEMLFID
                     PDECIDCDACVEACPVDACFAEDQLPAEWAHFAEKNAAYFQNA"
     gene            3826..4713
                     /locus_tag="PLJ64_03125"
     CDS             3826..4713
                     /locus_tag="PLJ64_03125"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNC92517.1"
                     /translation="MILLTGATGSIGSRLLPRLIEDGHQVRCLVREPRKLGAHRVDVQ
                     ITMGDLRELRDPYMLRQAMRGVDTVIHLAASIRDQPPYRVEELNGLATVRLLEAAERA
                     GVKRFVFFSALNASAVQRTRFFRAKWTAEQAVANSRLDSTIFRPSIVFDHSDPWITLL
                     RRFSFLPAIPISGNGKSRYQPVWADDVARAVENSLKGDGDGDHLYELAGPQTMTYAEM
                     SELVGLLAGRPRPVVRIPLQLVHLGLSAIHRLVGENAFATWQEAELMRVSMVTPRASE
                     DLESLGVQPRTMADVLADS"
     gene            4748..5791
                     /locus_tag="PLJ64_03130"
     CDS             4748..5791
                     /locus_tag="PLJ64_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92518.1"
                     /translation="MRFPARLSLATVVVVFALAAVADAGVFLPPKGKVYGGVSDNGTY
                     RGFENFSEATGKHPAVLQTYHPWGNQLFLAYQRWQNTEVLPMLHISTADDETRAELIT
                     PKEIARGVGDPYLIEINRFFSARRQKAFIRPLGEPNRCLNPYTAFTCSGKRRGGEHKT
                     IWYRRAFRRIALITRGGTTAGRLDRNLKKLRLPKLNWGSLTKSPKLPGAPVSIVWSTL
                     PSGSPSVRGNHPVDYWPGSKYVDWVGTDFYSNYPYWRDLNRFVHNRRWRQKPVALTEW
                     GVVGADDTRFARRVFAWVKQQPRVRMMVYYRGFGTADPYYPYNYPLTVKVIRNKLKSP
                     RFLAFAHGYARRR"
     gene            complement(5788..6819)
                     /locus_tag="PLJ64_03135"
     CDS             complement(5788..6819)
                     /locus_tag="PLJ64_03135"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HNC92519.1"
                     /translation="MDVTTRRLLAQGLIEPAAATPGQVVETLLAVQAQDGRGARLAIR
                     PRLSERVTEPSASMVDAALDEGSLVVNWLNRGTLHLVRAEDHGWLHALTAPRQATANQ
                     TRLRQEGVSEKQADRGVGMIVDRLKDGPATRGELKEVLAAEGVPVAGQALVHILLFAS
                     LQGLILRGPTLGGEHAFVLVEDWLGPQGEVDLELAAAELARRYLTGHGPATDRDLARW
                     AGVNLGLARKGLAAIAGETVEADGCLELKEKPAPTVAEAPVRLLGAFDPVLHGWTDRA
                     WILPDEETRKVVTTNGIFRPTILAGDRIVGTWTMPSGRVELAPFGKLDRKTVAALEVE
                     AERVRRYLG"
     gene            6868..7593
                     /locus_tag="PLJ64_03140"
     CDS             6868..7593
                     /locus_tag="PLJ64_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92520.1"
                     /translation="MKSEGFRRHLTYSNVMATIAVFAVLGGGAYAATKIPKNSVGARQ
                     LKKNAVTTAKLKNGAVTSAKVKKGSLLASNFKSGQLPRGATGPTGATGAAAVTVAAHG
                     GGEDPPALFGPTTNIVMASAPISLPRESDVVITGSMGDKQVECSVDGCQGPGGTDSTI
                     VGLYLEFPNSTGPSPVPGGGISVLGSSFNRIQIPPLSGVIRDIPPGIYTAKLILAGLG
                     ISTSVFDNGGADPWITALATDSG"
     gene            complement(7720..9357)
                     /gene="groL"
                     /locus_tag="PLJ64_03145"
     CDS             complement(7720..9357)
                     /gene="groL"
                     /locus_tag="PLJ64_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585059.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNC92521.1"
                     /translation="MAHKELKFAEDARGALQAGVDAVADAVKVTLGPKGRYVVLDKKF
                     GAPTITNDGVTIAREVEIEDVFQNQGAQLVREVATATNDVAGDGTTTATLLAQIIVRQ
                     GLKNVAAGANPLALRRGIEKAVDTVVANLREKQSAEVNGKDQIARVAAISAADDEIGN
                     TIADAIDKVGKDGVVNVEEGQTFGMELEFTEGMQFDKGYISPYMVTDQDRMEAVLEEP
                     YVLIANQKIGSVRDILPVLEQVMQGGKPLLIVAEDVEGECLATLVVNKLRGTFTGVAV
                     KAPGFGDRRKRMLEDIGILTGGEVITEEMGLKLENTQLTQLGQARRVVVSKDTTTIID
                     GAGDADAIKGRIKQIKSEIENTDSEFDREKLQERLAKLAGGVAVVKVGAATETEMKEK
                     KHRVEDALQAARAALEEGIVPGGGVALLNAQDALDVDSFEDADERTGAQIIHRSLEEP
                     VRQIAENSGLEGSVVVNKVRELKEGEGLNAANGEYGDMVKAGVLDPTVVTRSALQNAA
                     SIAKNILVTEAIIAEPPEEGGAGMPAMPDMGGMGGMM"
     gene            complement(9399..9686)
                     /gene="groES"
                     /locus_tag="PLJ64_03150"
     CDS             complement(9399..9686)
                     /gene="groES"
                     /locus_tag="PLJ64_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNC92522.1"
                     /translation="MKLKPLGDRLIVKPVDEEETTASGLVLPDTAKEKPQKGKVVAVG
                     DGAVADDGSRRPLDVAEGDEVLYSKYGGTDITVEGEELLVLRESDILAKVA"
     gene            complement(9918..10424)
                     /locus_tag="PLJ64_03155"
     CDS             complement(9918..10424)
                     /locus_tag="PLJ64_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNC92523.1"
                     /translation="MSGGGDPAAFAFASRWEVSAGRQAVWDALVDFNRWPVWWPALEE
                     VIETIHGDEEGIGQKAIAVWRGPVGYTLKMAIEAVEKVHPEFLRGVATGDVVGEGTWT
                     LATTADQWTSIEFDWNVRANRKWMEFLAPLARPLFVSSHDHVMKEGAEGLAGHLSCEM
                     RDFSASVG"
     gene            complement(10427..10822)
                     /locus_tag="PLJ64_03160"
     CDS             complement(10427..10822)
                     /locus_tag="PLJ64_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HNC92524.1"
                     /translation="MSGLLALNFYSPVFVDQLKRGRKTATIRLGNKSAKYQRSQIVWV
                     TVGSRHSRREKIFSAVIDEVEVKRLSELSPRDIERDNPEFRRVEETRDFLQKIYDREI
                     EEDAIVTIVHFSQIVEPGLTGYGTVPSPN"
     gene            complement(10832..11521)
                     /locus_tag="PLJ64_03165"
     CDS             complement(10832..11521)
                     /locus_tag="PLJ64_03165"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNC92525.1"
                     /translation="MTSAEESTVGLLRSVPLFAELDQDELEQFSRVAIPRAYPEGTRV
                     FHEGDHSDACYIVKSGTFRVTREHPDGRAITLATLTEGDIVGELAMLDGEVRSASVET
                     LGGDGELLALPASDVRALLERHPDITVKFVAALTRRLRAANERISRQSFQTVPSRVAG
                     VLSQLVAEEGEDGRQVTIRMNQADLAQLAGTSRESVSRFLADLERSGVVKAGRGQVTI
                     LEPARLGNYIF"
     gene            complement(11661..12497)
                     /locus_tag="PLJ64_03170"
     CDS             complement(11661..12497)
                     /locus_tag="PLJ64_03170"
                     /inference="COORDINATES: protein motif:HMM:NF018533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HNC92526.1"
                     /translation="MAEGDSLVRIARKLEPAMLGREIVAASAPNPRSTLRQHARRIDG
                     KRVERIETRGKHLLIHLDEGLTLHSHLGMNGGWRVDEGRGFGKPDRSAWLLLEMAGEE
                     GSVSATGGSPLATGSRTTRVAQFGGTTLRLVRTVELARDPRLATLGPDILAPEFDQVA
                     AAARLRVSPDEVGVALLDQKIVCGIGNIFKSEACFEARVDPWRPATSLAKGAGGEDEA
                     IRVLETARRQMQDAVETGKRPGRIYKKTGRPCPRCRAARIRSRGQGEDNRTTYWCPNC
                     QA"
     gene            complement(12584..17143)
                     /locus_tag="PLJ64_03175"
     CDS             complement(12584..17143)
                     /locus_tag="PLJ64_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNC92527.1"
                     /translation="MPEKPTVKSPPRDPLAPFSDDVRRWFSASFEGATPAQALGWQSI
                     SSGSDTLICAPTGSGKTLASFLWGIDRLARSHRAVAAGEAPEPEPGVRLVYVSPLKAL
                     SYDIERNLRAPLKGIGVDLSVGIRTGDTSQTERNRMRREPPEILITTPESLYLMLSSG
                     ARDILTSVETVIVDEIHAVAATKRGAHLALTLERLEHLVQENAGHGVQRIGLSATQRP
                     LERIAGFLSGPGRECEIVDPGQRKELDLEIVVPVEDMTAPEADQHDDGSADDAPIRSI
                     WPAIYPELLEQVRAHTSTIVFVNNRRAAERLAQRLNDLDQETGEIESPNRTATEGPRP
                     DQAGQPASGPEDGPKRPHEVEGLPLARAHHGSLSHEERTEVEEQLKSGLIPCLVATSS
                     LELGIDMGAVDLVVQVESPKSVARGLQRIGRAGHGLGETSKGRIFPKFRGDLLESAVV
                     TRRMREGLIEETVIPSNPLDVLAQHLVSMAALDKWDTTDAYELVRRTTPFAELSIEQF
                     DNVLDMLDGRYPSDRFAELRPRLVWDRTGKTIEGRKGARSLVVTNAGTIPDRGLYGVH
                     LPDGRRVGELDEEMVYEARAGQVFLLGASSWRIQEITRDRVIVIPAPGVPGAIPFWRG
                     DGIGRPAELGEAIGAFAREAVNAEPAELEKSHDLDPRAARNLIAYLKDQQDATRVVPS
                     DQTVVIERFRDEIGDWRLVVLTPWGGRVHAAWGLAIARKLRDEHGLEADAIHSDDGIA
                     VHLPDADEPPSADLVLIEPDELEDLVVRELGSSALFGARFRENAARSLLIPRAYPGKR
                     TPLWQQRLKSQSLLEVAQDFPRFPVILETYRECLQDVFDLPALDRLLRGIHSREISLV
                     EVETQTASPFASSLLFDYVATYMYEGDTPNAERRATALSLDRELLRELLGQDELRELI
                     DEGALAEVEASLQHLTEHGRAADRDGLQQILRRLGDLTAEEAEARVAEGYSAGSMLEN
                     LISERRAVKVRINGEERFIAAEDAGLYRDALGVAPPGGLPADFLEERPDAMETLLSRY
                     ARTHGPFPTAWPKERYGADPTPALKQLEAGAALVRGEIRPGGTEREWCDAEVLRRVRR
                     ASLAHLRQEVEPADGAAYARFLASWQGIDRHRPLRRDARPGAGTDRLREILVPLQGVA
                     LTQSVWENDVLARRLGSYSPTWLDELCTSGEVVWIGAGAVGRTDGRVALYFREDVRLA
                     GPPPANAKLTPPEGEVHDAIRERLAAGPAFWLDLANELDHPSEELHSALWDLAFGGEI
                     TNDSFAPLRAPRLRSVPTYEPGGRRFSRRRAATGPTVQGRWSLTETLFRNAPAAGPKL
                     RAQAELMLERYGILTREMALAEGIPGGFSTLYPELSNLEVLGTARRGYFVEGLGGAQF
                     ALSGAVERLRALPAEENGPDAFTVLAATDPASPWGSILPWPKLESGRKAARTAGAYVL
                     ARAGHPLLYVERGGKGILRLDPALEGEGLAAALATLIDEVEAGRVGQLKIERFDGEPI
                     LGSAFEQLLVAAGFSRQPRRLVAPA"
BASE COUNT         2703 a         5762 c         5770 g         2941 t
ORIGIN      
        1 cgggccgact cctcgaagta ctggatctgg atcaggttgc cgcgggtcgg ctgccaggct
       61 ccgcgggcgg caacccggcc ggtgaagtag acccgccggc cggtcctcag ccgcttcggc
      121 ctcgccttga agccgagccg gccccgcacc ctcagttcga gcacgcccga atccgatccc
      181 tggagccgcc tggttccggc gaagccgacg acaacccgac gacccggtcc gcgggccagc
      241 cagaggtcga agtagccgcg ggcatcggtc cggacctgcc ggctcgtcac cttggcccgg
      301 gagcccggga agggagtctc ggtgacggtc agcgtcgccc cgccgaggcc accgctctcg
      361 accccggtca gccggccttc gagatgggcc ctctccccgt aaccgatctc gaccgtgccc
      421 cggccccggc tggcccggct cgaacccagc gaggcggaaa cctgcgtctc gtccagcagc
      481 ggcgtgcgaa cgatcatctg cgagccgttg gcccggcggt cggtcacggt caggttgccg
      541 gccccgtcgg cgatcgccgc ccgcaacacc cagctgcctc gcggcaggtc atcggggaac
      601 cgggcttcga gcatctgccg gtcacccagg tagtcggatg agatcgggtg ccagtcgccg
      661 ccgtcggccg gccgccaggc gacgcttccg ccttgcaccc cggagaagcg gtcggcgacc
      721 gaaacccgga tcagggccgg gtcatcttcc ggggggtcga cgaagtaacc ggtcggggga
      781 acgttgtcga ggcggagcgt cgcttcggcc gcgtgggcct cgtcgctgtt gccggcctgg
      841 tcggccagcc agacccgggc tttgtattcc cccggtccgg gcagggtgat tccgctgatc
      901 tttcccgggt cggtccggta ccaggggccg ccggcatacc cgtcgggacc ggtcaggcgg
      961 aagtaactga ggacgatcgg ggaggccggc ccctgatcgg gttcggtcca ctcgaggttg
     1021 aagctgttgg tccgctgcca gacttcgccg ccttcgacgg tcagcccttt cggggcggcg
     1081 gggggattgt tgtcaacccg gatcgtgcgg tcggccgtgc agccgtagct gccggcgaaa
     1141 tcgaaggcgc agtgatgaag cgaatgcgga ccgtcggcca gggtccgggt gtcaaccgtc
     1201 tggctgtcgg tcgcggcggt cgggcagggc tgcatcttcg tgcccctcca ctggccggaa
     1261 atcatcgtct tggcgcagtt catttcggtg ctggcccgga gcgcgccgtc aatcgaggtc
     1321 tgtgcatagc gaagcccggc gccgacatcc cggttgctga aggtcagcga ctgggtcccg
     1381 cgcagccagt ccgttgcggc caacccaccg cttatctgcg ccgtcggcct gaccgcatca
     1441 tcgatcgaga tggtcagcga ccggaccccg gccagcaccg tcccgtcggc ctcgcagttc
     1501 ctgtccgaag tccggtaaca gaccagcctc gattcgaagg cctgggcgta gggctcgaaa
     1561 ccctgccaga aatcccgctt ggtcccgtcg ctgtagtcga gctcgagaaa aggggtgaag
     1621 ctgccgtttt gaggcacccc gccaagccgg tgctggaagc cttcacgcag gtactgccag
     1681 cgctggccgt ggacgttgac gatcccggtg ccgttcgggg cctgccagcg ccaggaggcg
     1741 aacttcgtcc cgccgaccga gtcgaccgaa ctcttgacct gactgatcag gtgaacgccc
     1801 tcgaaaggat cagccgaatc cggggtctgg cagtaggtgc tcttgccgaa gcggtcggcg
     1861 ctggaatcga accagctggc gtcctggccg acatgccagc cacactgggc gaccacgtat
     1921 ctggccgcgg cggcgccatc ggttgcggcg accgccgaga ttgcaaagag ggtgaatgcg
     1981 agcaaaatca ccgccagcgc ggcaaatcgg ttccggaatg ccattttggg ctcctcggtc
     2041 ggggtcatat gcctgactaa ggcgccgacc ggtcaaactc gccccccgcc cgcctttccg
     2101 ctcgcccggt ccgccctccg ccgcggcaga acgggcggct ctgcgagaat cagggcatgt
     2161 ccctcccgac cgaagagcag gtaaaagaga agctgaccgc ggtcatcgac ccggagcttc
     2221 gtcgcaacat cgtcgaactc ggcatggtcc gcagcgtcga aatcaaggat gacggccaga
     2281 tcaacgtgac cgtctcgttg accactccgg gctgtccgat ccgctcccat ttccaggaag
     2341 cggtggtcca gcaggtctcc tcgcttgacg gggtgaccgg ggtcggagtc ggcttcgatg
     2401 tgctgtcaga tgaagagaag tcaggcctgc gcgagacgct cggccggggc aagctccccg
     2461 agggcgcgct ggcccaggtc aagaacgtga tctgcgtcgc ctccggcaag ggcggggtcg
     2521 gcaagtcgac catgaccgcg aacctggccg ccgcgcttca ggccgaaggc aagaaggccg
     2581 gtgccctcga ctgtgacgtc tacggctact caatcccccg catgctcggg gtcgatcaga
     2641 agcccgaggt caactccgac cgcaagatcc ttcctccggt cgccgaaagc ggcgtcaaga
     2701 cgatgtcgat cggctacttc gtcgagcaga actccgcggt ggtctggcgc ggtccgatgc
     2761 tccacaaggc gattcagcag ttcctcgaag acgtcgcctg gggtgagctc gactacctgc
     2821 tgctggacct gccgcccggc accggtgaca tcgccatgac cctggcccag ctcctgccgc
     2881 aggcgaagat cacgatcgtg accacaccgc agccggccgc gcagtcggtc gcccggcgtt
     2941 cggccgaaat ggccgccaag gtcgacctcg aggtgatggg aatcatcgag aacatggccg
     3001 gcttcaccac cccggagggc gagaccttct cgatcttcgg cgaaggcggc ggtcagctcc
     3061 tggccgacga actcgaggtg ccgctgttcg gatcggtgcc gctctcggaa gagctccgcg
     3121 agcacgccga tgtcggcaac cccctctcgg tcgatcagcc ggattccccg gccggtcagg
     3181 cgatcgtcga aatcgcccgc aagatcatcg ccacctcacc cattgagctc ccgatgatcg
     3241 aaacgattca gaccaccgcc cccgaaatcg ccccgaaaat gggcaagaac gcccgcgaac
     3301 ttcccctcgt tcaataaaag ccgggcgggt tagccggtcc atcaaattcg ggatcaagtg
     3361 ggccggcggg catatgctcc gggtcggtgt tgccggccgg tgcgaaagct acgggagcgc
     3421 cagccctgct ggcgcgactg cagttaagcc tcaggcgttc tggaagtagg ccgcgttctt
     3481 ctcggcgaag tgggcccatt cggcggggag ctggtcctcg gcgaagcagg catcgaccgg
     3541 gcacgcttcg acgcaggcgt cgcagtcaat gcactcgtcg ggatcgatga agagcatttc
     3601 caccgcgtcg tagcccggct cgtcaggggt cgggtggatg caatccaccg gacacacctc
     3661 gacgcaggag ttgtcctttt gaccgatgca tggctctgcg atgacgtaac tcatggttcc
     3721 catattagag attgaggccg cccgacctcc ctctcgttga cccgccgatt agctgcctga
     3781 tcgcacggaa acggcccgca atcccgtcgc tttagactcc tgcccgtgat ccttttgacc
     3841 ggagcgacag gctcgatcgg ctcgcggctt ctgccgcgcc tgatcgaaga cggccatcag
     3901 gtccgctgcc tcgttcgcga accgcgcaag ctcggcgcgc accgggttga cgtccagatc
     3961 acgatggggg acctgcgcga actgcgtgac ccgtacatgc tgcgccaggc gatgcgcggg
     4021 gtcgacacgg tgattcacct cgccgcgtca atccgggacc agccgcccta ccgggtcgag
     4081 gaactgaacg ggctggccac cgtgcggctg ctcgaggcgg ccgagcgggc cggggtgaag
     4141 cgtttcgtct tcttcagtgc cctcaacgcc tcggcggtcc agcggacccg cttcttccgg
     4201 gccaagtgga cggccgagca ggcggtcgca aattcacggc tcgactcgac gattttccgg
     4261 ccctcgatcg tcttcgatca ttccgacccc tggatcaccc tgctgcgccg cttctccttc
     4321 ctgccggcga ttccgatctc cggcaacggc aagtcccgct accagccggt ctgggctgac
     4381 gatgtcgccc gggcggtcga gaactcgctc aagggggacg gcgatggcga ccacctgtac
     4441 gaactcgccg gcccgcagac gatgacctac gccgagatgt ccgagctggt cgggctgctg
     4501 gccggtcggc cccggccggt ggtccggatt ccgctgcagc tggtccacct gggtctttcg
     4561 gcaatccacc ggctggtcgg tgaaaatgcc ttcgcgacct ggcaggaagc cgagctgatg
     4621 cgcgtctcga tggtcacccc ccgggccagc gaggacctcg agtcactggg cgtccaaccg
     4681 cgcaccatgg ctgacgttct cgccgattcc tgaccgtccc gggcgcgggc tgcgatcatt
     4741 tctggcagtg aggtttccgg cccgtctcag cctggcgacg gtcgtcgtcg ttttcgctct
     4801 tgccgcagtc gccgacgcgg gcgtcttcct gccgccgaag ggcaaggttt acggcggggt
     4861 aagcgacaac ggcacctacc gcggtttcga gaacttctcc gaagcgaccg gcaagcaccc
     4921 ggcggtcctt cagacctacc acccgtgggg aaaccagctc ttcctcgcct accagcgctg
     4981 gcagaacacc gaggtgctgc cgatgctcca catctcgacg gctgacgatg agacgagggc
     5041 agagctgatc accccgaagg agatcgcccg gggcgtcggc gacccctatc tgattgagat
     5101 caaccggttc ttctcggcca ggcgccagaa ggccttcatc aggccgctgg gtgaaccgaa
     5161 ccgctgcctc aatccctaca ccgccttcac ctgctcgggg aagcggcgcg gcggcgagca
     5221 caagacgatc tggtaccggc gcgcgtttcg ccggatcgcc ctgatcaccc ggggcgggac
     5281 cacggcgggc cggctcgacc gcaacctgaa gaagctccgc ctgccgaagc tgaactgggg
     5341 ctcgctgacc aagagcccga agctgcccgg ggctccggtg tcgattgtct ggagcaccct
     5401 tccgtcgggc tccccgtcgg tccgaggcaa ccatccggtc gactactggc ccggaagcaa
     5461 gtacgtcgac tgggtcggca ctgacttcta ttcgaactat ccctactggc gggacctcaa
     5521 ccgcttcgtc cacaatcgcc gctggcgaca gaagcccgtc gccctgaccg agtggggcgt
     5581 ggtcggggcc gacgacaccc gcttcgcccg ccgggtgttc gcctgggtga agcagcagcc
     5641 gcgggtccgg atgatggtct actaccgcgg cttcggaacc gccgacccct actacccgta
     5701 caactacccg ctcacggtca aggtgatccg caacaagctg aagagtcccc gctttctcgc
     5761 cttcgcccac gggtacgcga ggcgccgcta accgagatag cgcctcactc tttcggcttc
     5821 gacttcgaga gctgccaccg tcttccgatc gagctttccg aagggcgcga gttcaaccct
     5881 gccggacggc atcgtccagg taccgacgat ccggtctccg gcaaggatgg tcggccggaa
     5941 gatcccgttg gtggtgacga ccttccgggt ctcttcgtcc gggaggatcc aggcgcggtc
     6001 agtccagccg tggaggacgg gatcgaaggc accgagcagc ctcaccggcg cctcggccac
     6061 cgttggcgcc ggtttttctt tcagttcgag gcatccgtcc gcttccactg tttcgccggc
     6121 gatcgccgcc aggcccttgc gggccagacc gaggttgact ccggcccacc tggcgaggtc
     6181 acgatcggtt gccggtccgt ggcccgtgag gtaccggcgc gccagttcgg cggcggccag
     6241 ttcgaggtca acctcgccct gcggcccgag ccagtcttcg accaggacga aggcgtgctc
     6301 gcctccgagc gtcggtccgc gcaggatcag accctgaagg gaggcgaaca gcagtatgtg
     6361 aaccagcgcc tggccggcga ccggcactcc ctccgccgcg agaacttcct tcagctctcc
     6421 gcgggtggcc ggcccgtcct tcagccggtc gacgatcatc ccgactcccc ggtcggcctg
     6481 cttctccgag acgccttcct gcctcaggcg ggtctggttc gcggtggctt gccggggcgc
     6541 ggtcagcgcg tgcagccagc cgtggtcttc ggcccgaacc aggtggaggg tgccgcggtt
     6601 cagccagttg acgaccagcg acccctcgtc gagcgcggca tcgaccatcg aggccgatgg
     6661 ttccgtgacc cgctcgctca gccgtggccg gatcgccagc ctggctcccc ggccgtcctg
     6721 ggcctgtacg gcgagcagcg tttcgactac ctgaccgggg gtcgccgcag ccggctcgat
     6781 cagaccctgg gcaagcagcc gtctggttgt cacgtccacg ggtcaaggct atggtggatt
     6841 cacggttgaa cgacttgagg gggagcgatg aagtccgaag gattccggcg gcacctgacc
     6901 tattcgaacg tgatggccac tatcgccgtg tttgcggtcc tcggtggcgg cgcctatgcg
     6961 gcaacgaaga ttccgaagaa cagcgtcgga gccaggcagc tcaagaagaa cgccgtcacc
     7021 accgccaagc tgaagaacgg tgcggtcacc tcggccaagg tgaagaaggg ctcgctgctc
     7081 gcttcgaatt tcaagtccgg tcagctgcca cgcggagcga ccggaccgac cggcgcgacc
     7141 ggtgccgccg cggtcaccgt ggcggcgcat ggaggcggtg aggatccgcc cgccctgttc
     7201 ggcccgacca cgaacatcgt gatggcctcc gcgccgatca gtctccccag ggagagtgac
     7261 gtggtgatta cgggttcgat gggtgacaag caagtcgagt gttcggtcga cgggtgccag
     7321 gggcccggag gaaccgattc gacgatcgtc ggcctctatc tggaattccc taactcgacc
     7381 ggaccctccc cggtccccgg cggcggaatc tcggttctgg gcagcagctt caaccgaatc
     7441 cagattcccc cgctctccgg ggtgatccgg gatatcccgc ccggcattta cacggccaag
     7501 ctgatcctcg ccggcctcgg aataagcact tccgtcttcg acaacggcgg cgccgacccc
     7561 tggatcaccg ccctcgccac ggactccggc taggaggcat gcatctgggt tgaagttatc
     7621 cgcacatgga acgaataact tcaacccaga tgcccatgca atgaaaaagg cccgggattt
     7681 ctcccgggcc cttctcggaa atctctgaaa gagacttcct tacatcatgc cgcccattcc
     7741 gcccatgtcg ggcatcgcgg gcatgccggc gccaccttcc tccggcggct cggcgatgat
     7801 cgcctcggtc accaggatgt tcttggcgat cgacgcggcg ttctggagcg cggagcgggt
     7861 cacgacggtc gggtcgagca cgcccgcctt gaccatgtca ccgtactcgc cgttggctgc
     7921 gttcaggcct tcgccttcct tcagctcgcg gaccttgttg accacgaccg aaccctcgag
     7981 accggagttc tcggcgatct gccggaccgg ctcttcgagc gagcggtgga tgatctgcgc
     8041 accggtgcgc tcgtcggcat cctcgaagga gtcgacgtca agcgcatcct gggcgttcag
     8101 gagagcgaca ccgccgcccg ggacgatgcc ctcttccagt gccgcacggg cggcctggag
     8161 agcgtcctcg acgcgatgct tcttctcctt catctcggtc tcggtggcag cgccgacctt
     8221 gaccacggca acgccgccgg ccagcttggc gaggcgctcc tggagcttct cgcggtcgaa
     8281 ctcggaatcg gtgttctcga tctccgactt gatctgcttg atccggccct tgatggcgtc
     8341 ggcatcgccg gcaccgtcga tgatggtggt ggtgtccttc gagacgacca cgcggcgggc
     8401 ctggccgagc tgggtcagct gggtgttctc cagcttgagg cccatttcct cggtgatgac
     8461 ttcgccaccg gtcaggatgc cgatgtcctc gagcatgcgc ttgcggcggt cgccgaagcc
     8521 cggcgccttg accgcaacac cggtgaaggt gccgcgcagc ttgttgacca cgagggtcgc
     8581 gaggcattcg ccctcgacgt cctcggcgac gatcagcagc ggcttgccgc cctgcatgac
     8641 ctgctcgagc acgggcagga tgtccctgac ggaaccgatc ttctggttgg cgatcagcac
     8701 gtacggctct tcgagcacgg cttccatgcg gtcctggtcg gtgaccatgt agggcgagat
     8761 gtagcccttg tcgaactgca tgccctcggt gaactcgagc tccatgccga aggtctggcc
     8821 ttcttcgacg ttgaccacgc cgtccttgcc gaccttgtcg atcgcgtcgg cgatcgtgtt
     8881 gccgatctcg tcgtcagcag ccgaaatggc ggcgacgcgg gcgatctggt ccttgccgtt
     8941 gacctctgcc gactgcttct cgcgcaggtt ggcgacaacg gtgtcgactg ccttctcgat
     9001 gccgcggcga agcgccagcg ggttggcgcc ggcggccacg ttcttgaggc cctggcggac
     9061 gatgatctgg gcgagcaggg tggcggtggt ggtgccgtca ccggcaacat cgttagtcgc
     9121 ggtcgcgacc tcgcgcacca gctgggcgcc ctggttctgg aagacgtcct cgatctctac
     9181 ctcgcgggcg atcgtgacac cgtcgttggt gatggtcgga gcgccgaact tcttgtcgag
     9241 cacgacgtag cgacccttgg ggccgagggt taccttgact gcgtcagcga cggcgtcaac
     9301 gccggcctgc agggcacccc tggcatcttc tgcgaacttc agctctttgt gagccatatc
     9361 tttctaatac ctcctgaaag tctggttgtc aggacgcgct aggcgacctt ggcgaggatg
     9421 tccgactcac gcaggacgag aagctcttcg ccctcgacgg tgatgtcggt tccgccgtac
     9481 ttggagtaga ggacctcgtc gccttcagcg acgtcgagcg gacgacggct gccgtcgtcg
     9541 gcgaccgcgc cgtcaccgac agcgacgacc ttgcccttct gcggcttttc cttggcggtg
     9601 tccgggagga ccagaccgct ggcggtcgtc tcctcttcat ctaccggctt gacgataagc
     9661 cggtccccga ggggcttcag cttcattgat tacctccgat ggtcatgttc atacgtgttt
     9721 caggttccat acatcgcttc tggcgccggg aaatcgatga ttcgggcgcc tcagtctctt
     9781 ggcactctcg gggggagagt gccagcaact gacaaccata cagaattgag accgtcggtc
     9841 aaccccgaaa tgtgaaaaat ctgagtccac tcgactcaag ccggggaaac ccggctccag
     9901 tccgcaagca gaggggctca gccgacggaa gccgaaaagt cacgcatctc gcatgagaga
     9961 tgaccggcga ggccttcggc tccctctttc atcacatggt cgtggctgga gacgaagagg
    10021 gggcgtgcga gcggggcgag gaattccatc cacttgcggt tggcccgtac gttccagtcg
    10081 aattcgatcg aggtccactg gtctgccgtg gtcgcgaggg tccacgtacc ttccccgacc
    10141 acgtcgccgg tcgccactcc gcgaagaaac tccgggtgga ccttctcgac cgcttcgatc
    10201 gccatcttca gggtgtaacc gaccggtccg cgccagaccg cgatcgcctt ctggccgatc
    10261 ccttcctcgt caccgtggat cgtctcgatc acctcttcga gcgcaggcca ccagaccggc
    10321 caccggttga agtcgaccag ggcatcccac accgcctggc gaccggccga aacctcccag
    10381 cgggaagcaa aggcgaaagc tgccgggtcg ccccctcccg acatggctag ttgggcgagg
    10441 ggacggtgcc gtagccggtc aggccgggtt cgacgatctg ggagaagtgg acgatggtca
    10501 cgatcgcgtc ttcctcgatc tcgcggtcgt agatcttctg gaggaagtcc cgcgtctcct
    10561 cgacccggcg gaattccggg ttgtcgcgct cgatgtcccg gggcgagagt tcggacagcc
    10621 gcttgacctc gacctcgtcg atcaccgcag agaagatctt ttcccgcctg gagtgccggg
    10681 aaccgaccgt gacccacacg atctggctgc gctggtactt ggcggacttg ttgccaagcc
    10741 ggatcgtggc ggtcttgcga ccccgcttga gctggtcgac aaacacaggg gaatagaagt
    10801 tgagagccag gagtccggac atttgcccga tctagaatat gtagttgccg agccgggcgg
    10861 gttcgaggat ggtcacctgg ccccgccctg ccttcaccac gcctgacctt tccagatcag
    10921 ccaggaaccg gcttacgctt tcgcgggagg tgccggccag ctgggccagg tcggcctgat
    10981 tcatccggat cgtcacctgg cggccgtcct ccccctcctc ggcgaccagc tgggaaagga
    11041 ccccggccac ccgtgatgga acggtctgga aggactgccg ggaaattcgt tcgtttgccg
    11101 ctcgcaggcg cctggtcagc gcggcgacga acttgacggt gatgtcggga tgcctttcga
    11161 gcagggcccg cacgtcgctc gcgggcaggg cgagcagctc gccgtcaccg cccagggtct
    11221 cgaccgaggc ggaacgcacc tcgccgtcga gcatcgccag ttcaccgacg atgtcccctt
    11281 cggtcagggt tgcaagggtt atggccctgc cgtccggatg ctcgcgggtt acccggaagg
    11341 tgccggactt gacgatgtaa caggcgtcgg aatggtctcc ctcgtggaag accctggtcc
    11401 cttcggggta tgcacgcgga atcgcgacac gcgaaaactg ctcgagctcg tcctgatcaa
    11461 gctcggcgaa aaggggcacg cttctcagca gcccgactgt gctctcctca gccgaagtca
    11521 cccgcggagt atttcgcatc cgaccggaaa aacaaaacga ttctgatcaa ttgaggtcag
    11581 aaacccttcc caggtgcctt ccgggggcgt ttacccacca aaacggtggc cgatcgcccc
    11641 cggtttggac gggagtcgga tcaggcctgg cagttcgggc accagtaggt ggtgcggttg
    11701 tcttctccct ggccgcggga gcggattctg gccgcgcggc agcgggggca ggggcggccg
    11761 gtctttttgt agatgcggcc cggccgcttg ccggtctcga ctgcgtcctg catctgccgg
    11821 cgggcggttt cgagtacacg gatcgcctcg tcctcgcctc cggcaccctt cgccagggag
    11881 gtggccggcc gccaggggtc gaccctcgcc tcgaagcagg cttcggactt gaagatgttg
    11941 ccgatcccgc agacgatctt ctggtcaagc agggcgactc cgacctcgtc cggactgaca
    12001 cggagccgcg ccgccgcagc cacctggtcg aattccgggg cgaggatgtc gggaccgagc
    12061 gtcgcaagcc gcgggtcgcg ggccagctcg accgtgcgga ccaggcgcag ggtcgtgccg
    12121 ccgaactggg caacccgcgt cgttcgtgag ccggtggcca acgggctgcc accggtcgcg
    12181 ctcacactcc cttcctcacc tgccatttcc agcagcagcc aggcactgcg gtcgggcttg
    12241 ccgaagccac ggccttcgtc cacccgccag ccgccattca tgccgagatg ggaatggagg
    12301 gtcagccctt cgtcgagatg aatcagcaga tgcttgccgc gggtttcgat ccgctccacc
    12361 cgcttgccgt cgatgcggcg ggcatgctgg cgcagagtgg agcggggatt cggtgccgag
    12421 gccgccacga tctcccggcc gagcatcgcc ggctcgagct tccgggcgat gcgaaccagc
    12481 gaatcacctt ctgccatagg tcagctcgtc ctcgttccgg gtgacatttg ggtggttata
    12541 gcgacccaaa tgtcacccgg aatgtgctct acgccagttg cgttcacgcg ggggcgacca
    12601 ggcggcgggg ttggcggctg aagccggccg cgaccagaag ttgctcgaag gcggagccga
    12661 ggatcggttc gccgtcgaat cgctcgatct tgagctggcc gacccggccg gcctcgacct
    12721 cgtcgatcag ggtggcgagg gcggcggcga ggccttcacc ctcaagggcc ggatcgagcc
    12781 ggaggatgcc cttgccgccc cgctcgacgt agagcagcgg atgaccggcc cgggccagta
    12841 cataggcgcc ggcggtgcgg gcggccttgc ggcccgactc gagctttggc cagggcagga
    12901 tcgaacccca ggggctggcc gggtcggtgg cggccagcac ggtgaaggcg tccgggccgt
    12961 tctcctcggc cggcagggcc cgcagccttt cgaccgctcc ggagagggcg aactgggcgc
    13021 cgcctaggcc ttcgacgaaa tagccgcgcc gggcggtacc cagcacctcg aggttggaga
    13081 gttccgggta gagggtggag aagccgccgg gtatgccctc ggccagggcc atctcccggg
    13141 tcaggatgcc gtagcgctcc agcatcagct cggcctgggc ccgcagcttc gggccggcag
    13201 cgggggcgtt gcggaacagg gtctcggtaa gtgaccagcg gccctggacg gtcgggccgg
    13261 tggcggcacg gcgacgcgag aagcggcggc cgcccggctc gtaggtcggg accgaccgga
    13321 gccgcggcgc ccggagcggc gcgaaggagt cgttggtgat ctcgccaccg aaggcgaggt
    13381 cccagagggc cgagtgcagt tcctcggaag ggtgatccag ctcgttggcg aggtcgagcc
    13441 agaaggccgg gccggcggcg agccgctcgc ggatcgcgtc atggacttcg ccctccggcg
    13501 gggtcagctt cgcgttggcc ggtggcgggc cggccagccg cacgtcctcc cggaagtaga
    13561 gcgcgacccg tccgtcggtg cggccgaccg ccccggctcc gatccagacc acctcgccgc
    13621 tggtgcagag ctcgtccagc caggtcgggc tgtaggagcc gagccggcgg gccagcacgt
    13681 cgttttccca taccgactgg gtcagggcca cgccctgcag cggaaccagg atctcgcgca
    13741 gccggtcggt tccggccccg gggcgggcgt cacggcggag cgggcggtgg cggtcgattc
    13801 cctgccatga ggcgaggaag cgggcgtagg cggcgccgtc ggccggttcg acctcctgcc
    13861 tgaggtgagc gaggctggcc cgccggaccc ggcgcagcac ttccgcatcg caccattcgc
    13921 gctcggtgcc gcccgggcgg atctcgccgc gaaccagggc ggcgcccgct tcgagctgct
    13981 tgagggcagg ggtcggatcc gccccgtagc gctccttcgg ccaggcggtc gggaacgggc
    14041 catgggtcct cgcgtagcgg ctcagcaggg tttccatcgc gtccggtcgt tcctcgagga
    14101 agtcggccgg cagccccccg ggcggagcca cgccgagagc gtcccggtaa agaccggcat
    14161 cctcggcggc gatgaaccgc tcttcaccgt tgatccggac cttgaccgcg cggcgctcgg
    14221 agatcaggtt ttccagcatc gaaccggccg aatacccttc cgcgacgcgg gcctcggcct
    14281 cttcggcagt caggtcgccg agccggcgga ggatctgctg gaggccgtcg cggtcggcgg
    14341 cccggccgtg ctcggtcagg tgctggagcg atgcctcgac ctcggccagg gctccctcgt
    14401 cgatcagctc gcgcagctcg tcctggccga gcagctcgcg caggagttcg cggtccagcg
    14461 aaagagcggt ggcccggcgt tccgcgttcg gggtgtcccc ctcgtacatg taggtagcga
    14521 cgtagtcgaa gagcagcgag gaggcgaagg gcgaggcggt ctgggtctcg acctcgacca
    14581 gcgagatctc ccgcgagtga atgccgcgca gcagccggtc cagcgcgggc aggtcgaaga
    14641 cgtcctgcag gcactcgcgg taggtctcga ggattaccgg gaagcggggg aagtcctggg
    14701 cgacctcgag caggctctgg gacttgagcc gctgctgcca gagcggtgtc cgcttgccgg
    14761 ggtaggcgcg ggggatcagc agcgaacggg ccgcgttctc gcggaagcgg gccccgaaga
    14821 gagcggagga accgagttcg cggaccacga ggtcctcgag ttcgtccggt tcgatcagga
    14881 ccaggtcagc cgagggcggc tcgtcggcat cgggcaggtg gacggcgatg ccgtcatcgg
    14941 aatggatcgc gtcggcttcg agcccgtgct cgtcgcggag cttgcgggcg atcgcgaggc
    15001 cccaggccgc gtgcacccgt ccgccccagg gggtgagtac gaccagccgc cagtccccga
    15061 tctcgtcgcg gaagcgctcg atcacgaccg tctggtccga gggcaccacc cgggtcgcgt
    15121 cctgctggtc cttcaggtag gcgatcaggt tgcgggctgc ccgcggatcg agatcatggg
    15181 acttctccag ctcggccggt tcggcgttga ctgcctcgcg ggcgaaggcg ccgatcgctt
    15241 cgcccagttc ggccggccgg ccgatcccgt caccgcgcca gaacgggatc gcccccggca
    15301 cacccggggc cgggatcacg atcacccggt cacgggtgat ctcctgaatc cgccagctcg
    15361 aggcgccgag caggaagacc tggccggccc gtgcctcgta gaccatctcc tcgtcgagct
    15421 cgccaacccg gcggccgtcc ggcaggtgaa ccccgtagag gccgcggtcg gggatcgtgc
    15481 cggcgttggt cacgaccagc gagcgggcgc ccttgcgtcc ttcgatcgtc ttgccggtcc
    15541 ggtcccagac caggcgcggc cggagctcgg cgaaacggtc cgacgggtag cggccgtcga
    15601 gcatgtcgag cacgttgtcg aactgctcga tcgagagctc ggcgaagggc gtggtgcggc
    15661 gaaccagctc gtaggcatcg gtcgtgtccc acttgtcgag cgccgccatc gaaaccaggt
    15721 gctgggcgag cacgtcgagc ggattcgacg ggatcaccgt ctcctcgatc aggccttccc
    15781 gcatgcggcg ggtaaccacc gcgctttcga gcaggtcgcc ccggaacttg gggaagatcc
    15841 gccctttcga ggtttccccc aggccgtggc cggcccggcc gatccgctgc aggccgcgcg
    15901 cgaccgactt cggcgattcg acctggacca cgagatcgac cgcgcccatg tcgatcccga
    15961 gctcgagcga ggaggtggcg accaggcagg ggatcagccc ggacttgagt tgctcctcga
    16021 cctcggtccg ctcctcgtgg gagagcgaac cgtgatgggc ccgggccagg ggcaggccct
    16081 ccacctcgtg gggccgcttc ggtccgtcct ccggcccgga cgccggttga ccggcttgat
    16141 ccgggcgggg cccttcggtc gccgtgcggt tcggcgattc gatctcgccg gtttcctggt
    16201 cgaggtcgtt cagccgctgg gcgaggcgct cggcggcacg gcggttgttg acgaagacga
    16261 tggtcgaggt gtgagcgcgg acctgctcga gcagctccgg atagatcgcc ggccagatcg
    16321 accggatcgg ggcatcgtcc gcggagccat cgtcgtgctg gtcggcttcg ggtgcggtca
    16381 tgtcctcgac cggcaccacg atttccaggt cgagttcctt gcgctggccc gggtcaacga
    16441 tttcgcattc gcgaccgggg ccggaaagga agccggcgat gcgctcgagg ggccgctggg
    16501 tggccgagag gccgatcctc tgcaccccgt ggccggcgtt ttcctgaacg aggtgttcca
    16561 gccgctcaag ggtcagggcg aggtgggcgc cgcgcttggt cgcggcaaca gcgtgaatct
    16621 catcgacgat cacggtctcg accgaggtga ggatgtcccg ggcgcccgag gaaagcatca
    16681 ggtagagaga ctccggggtg gtgatcagga tctccggcgg ttcgcgccgc atccggttgc
    16741 gttcggtctg ggaagtgtcc ccggtccgga tcccgaccga aaggtccacc ccgatcccct
    16801 tcagcggggc gcgcaggttg cgctcgatgt cgtaggaaag cgccttgagc ggcgatacat
    16861 agaccagcct cacccccggt tcgggctcgg gcgcttcccc ggccgcgacg gcccggtggc
    16921 tgcgggcgag ccggtcgatc ccccagagga agctggccag ggtcttgccg gagccggtcg
    16981 gagcgcagat caacgtgtcc gagccggagg agatcgactg ccagccgagc gcctgggccg
    17041 gggtcgcgcc ttcgaacgaa gccgaaaacc agcgtcggac atcgtcgctg aagggagcga
    17101 gcgggtcccg gggaggtgat ttcacggtcg gcttttcagg catcgctaaa cccctgatgc
    17161 tacgcccccg cccacc
//
LOCUS       DAOQKN010000027        17058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_1523_length_17058_cov_5.704405, whole genome shotgun sequence.
ACCESSION   DAOQKN010000027 DAOQKN010000000
VERSION     DAOQKN010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 17058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17058
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1523_length_17058_cov_5.704405"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(192..536)
                     /locus_tag="PLJ64_03180"
     CDS             complement(192..536)
                     /locus_tag="PLJ64_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC92528.1"
                     /translation="MSAIDNDQCPVCRTADVISGKWTLLVIRDLAGESRRFCELERSL
                     EGISPRTLSLRLRTLEEAGIVERQTFPEVPPRVTYALTDKGKDLVPLIDDMREYGIRW
                     LEVEEPSQTVSA"
     gene            complement(623..1051)
                     /locus_tag="PLJ64_03185"
     CDS             complement(623..1051)
                     /locus_tag="PLJ64_03185"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HNC92529.1"
                     /translation="MAVPVLVITLLIASATDLRSREIPNWLMAGAALAGIGLAAAEGR
                     AGSAVLFGLLAAAPFLLANLIRPEGMGMGDVKLVAVVGLYLGQQVWIALVIGLALAGL
                     TGVLIALGRRLPPSRTALPLAPFLAAGAGIMLAATGGALQ"
     gene            complement(1164..2267)
                     /locus_tag="PLJ64_03190"
     CDS             complement(1164..2267)
                     /locus_tag="PLJ64_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNC92530.1"
                     /translation="MLVAELQELEEVKTLVNRGQQLGVLTFGDVATAVAEVDLDESDV
                     EDLYGHIEKQGIELVEDVDPAQKASAENERSDGRRGRRRKKEALDLKPDMTTDSLQLF
                     LKDIGKVRLLTAQEEVDLAKRIERGDLDAKQKMVESNLRLVVSIAKNYRNQGLPFLDL
                     IQEGTLGLVRAAEKFDYRKGFKFSTYATWWIRQAIARALADKARTIRIPVHVVEKLNK
                     IGRAERKLVTELGREPTPEEIAEVTGIDPEEVDSIKRSAQSPVSLEKPVGDEEESEFG
                     QFIADEKAESPFDRAADLLTKEALKESLENLSYRERRVLELRYGLGGEHPRTLDEVGR
                     TFNVTRERIRQIENQSLKKLQSLNEAQKLREAT"
     gene            2518..3612
                     /locus_tag="PLJ64_03195"
     CDS             2518..3612
                     /locus_tag="PLJ64_03195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNC92531.1"
                     /translation="MSCTDCDENHPEHPNDAPLPLSEQWKHLHWLQAFWTRAWRENVT
                     GLSGMVAYNLMLAIFPFAFLVLFIFSQVLRVEGIDQGIINDLQRLFPNAEQGTLDDIL
                     KAIRNNSATLGVAAVFGSLWIGASFWGAMDTAFCRIYHVQCRGWWEQKRFSLVMLLVV
                     TIFLLGSVLLPALEGALIRQTDNLPFGLNEVQNLDNAVLLAVTLSLNFTVAAVIYWAV
                     PKGHMPWRAVWPGALFTTLVAAAANWLFPVYLNNVSTLNRFGSTIGFILIALFWFYLV
                     ALTMLAGAVINSIRHEYHDTGSLPYGILSRAQLLARAQADEAKAAQRPMAEPYRSLVS
                     GDAWPKPEAGPDEESEAETKVIPLRRRKRQ"
     gene            3609..4214
                     /locus_tag="PLJ64_03200"
     CDS             3609..4214
                     /locus_tag="PLJ64_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92532.1"
                     /translation="MSVRIELMPDPVRHGLARTGGVTCEQEAVITVDRETFEQIWTPS
                     TLELLARSYWDYIRRFTLGAIRVQYRVDSQTVTLLGRVPLLRFRTPVFSTGNDRASIE
                     WPVEKGLLVARDGRGKGFLRIEASRGQAEDAGCPTLMVSSTVSNFYPWLRGTGRFARF
                     GTWLYSQTQLRIHILVTKGFLRSLDELPDEVLRRGVSPGTG"
     gene            complement(4211..5122)
                     /locus_tag="PLJ64_03205"
     CDS             complement(4211..5122)
                     /locus_tag="PLJ64_03205"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNC92533.1"
                     /translation="MASSAEPVLVAGSTGYIGRRLALRLVRGGNAVRCMARNPARAGD
                     LEEAGCEVVAGDVLDRESLRWPLSGVRVAYYLVHSMGRGASGDFAESDLKGASNFAEA
                     AAEVGVERIVYLGGLGEGRSRHLRSRHETAQALGSTGIDVTYMRAAVVIGAGSESFQT
                     IYWLVRRLPAMVTPRWTSVRTQPIGIDDVVECLARALKLDGNSEIQIGGPDVTTYGGL
                     MDETAKAMGRRPPLRIGVPFLTPRLSSLWLGLITPVDVGVARPLIEGLSTETVVTQPP
                     GPEELGVQPESLGTVLRKAVAEIEAGG"
     gene            complement(5106..6119)
                     /gene="glpX"
                     /locus_tag="PLJ64_03210"
     CDS             complement(5106..6119)
                     /gene="glpX"
                     /locus_tag="PLJ64_03210"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HNC92534.1"
                     /translation="MGVISHDVRSGSKPDRNLALELVRVTESAALAASRWIGRGDKLA
                     ADGAAVDAMRILLDTVPMDGIVVIGEGEKDEAPMLYNGEKIGDGSPPVVDIAVDPLEG
                     TTLTARGFGGALAVIALSERGTMFDPGPCVYMEKLAAASEFKDLLTFDEPIGEVIRKI
                     GERKGGGPEDVTLTILDRPRHEEVVKEVRESGATIRFITDGDVQAALLAVSDDTGIDL
                     LWGIGGTPEGVLAAAAIKCLGGEIIGKLWPRNDEERQAAIDAGYDLDRVLTADDLVSG
                     DDVFFAATGVTDGDLLDGVRRHKGWASTESLVMRSRSGTVRKIGARHDRAKLREVTGG
                     LFG"
     gene            6147..7079
                     /locus_tag="PLJ64_03215"
     CDS             6147..7079
                     /locus_tag="PLJ64_03215"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNC92535.1"
                     /translation="MSARWGLTLPLPGVSLADTKDFVQLAEAEGYTDLWTGETGGPDG
                     FTPLALSAAWTSKVRLGTGIVGVFQRGPALLAQQAAALTDASSGRFALGIGASSDRMV
                     EGWNQIPFVKPLSKVEQTVDFLRVALAGERTDTGFKLETAPTHETPIVLAALRGKMLE
                     LAVNKADGAFTNFLPLAGLPKVVDGIEGAGNDFELLCRFFCIPGEKEQVEGLARFMFS
                     SYITVPVYANFFRWLGYGEEIEAMVEHWNNKDRQAAAAAAPWELIEDTFLLGSPEQIR
                     ARLDEYVAGGITLPVITPVTTPDNHAAMIKALAP"
     gene            complement(7177..8079)
                     /locus_tag="PLJ64_03220"
     CDS             complement(7177..8079)
                     /locus_tag="PLJ64_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92536.1"
                     /translation="MGVRLKSTRGQSTIEWVGLITLVAALMAALTAAGIQVPAVGLAH
                     SVSTRLLCAASLSGSCAGEGSLDEAYGPEVAELVRSGAPALMYGKDMLGLPVDYRTCR
                     SPWCADGTGEGEVSRSTAGEPVTLFTRVIHEPDGSIYIQYWAYYPESASLRGAPLLED
                     EGYHRNDWESMQIRVDPDGVVSERASSHAGYNHTRSSVNWGSDLGWGFLTDAAEKVGL
                     REKGGWGDATGQYLIAGGSHAANVAASLADSDYPSYTPSALVRLVPLESVKDGPLARP
                     ADFDPITPPWEKEVWTEPEAEGTG"
     gene            8163..8441
                     /locus_tag="PLJ64_03225"
     CDS             8163..8441
                     /locus_tag="PLJ64_03225"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HNC92537.1"
                     /translation="MADRNKMTPPSSASKKTPEWKWWAFGIAVLLLLIVVLQNSQDVE
                     FKILFLVDTAAPLFVLLLFAAAIGAIIGYTGPILRRHRHNTRKEYGKD"
     gene            8462..9064
                     /locus_tag="PLJ64_03230"
     CDS             8462..9064
                     /locus_tag="PLJ64_03230"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNC92538.1"
                     /translation="MIEPVRLEGDRVALEPIRADLADDLWTAAQDPEIWHWTTHLNHS
                     REFFDGWVAGGIEGWESGERSTFVTSLRSAGSDEPARVVGSSSYLFYRPDEDVVEIGS
                     TWLNPGAWGTGANTEAKLLMMTHAFETLGCVRVEFKTDRRNERSRAALAALPAQFEGI
                     LRKHTNPFGVGRRDSAYYSVIDDEWPEVRAALEARLARRS"
     gene            complement(9087..9902)
                     /locus_tag="PLJ64_03235"
     CDS             complement(9087..9902)
                     /locus_tag="PLJ64_03235"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNC92539.1"
                     /translation="MERNRILLGDNLDLMAGLAAGSFQMVYADPPFNTGKQQSRQALR
                     TVADTDGDRTGFGGRRYRTEKAGEASYSDAFDDFTAFLEPRLKQIHRLLNETGMLYLH
                     IDYREAHYVKVMLDGIFGRESFLNEIIWAYDYGGKSKRRWPAKHDTILVYVKDPDRYY
                     FDSGEVDREPYMAPGLVTPEKVAKGKLPTDVWWHTIVPTNGAEKTGYPTQKPEGIVRR
                     MVLASTRPGDLCLDPFCGSGTLGAVCRKLGRDYLLIDENPEAVEIAGKRIGQD"
     gene            9983..10855
                     /locus_tag="PLJ64_03240"
     CDS             9983..10855
                     /locus_tag="PLJ64_03240"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC92540.1"
                     /translation="MTASSDLPYPPFELANRVIELPSNDFAGYVHYEMAGRQTRDELL
                     DLLPDGYTLEGRSLLDFGCGAGRTLRQFHDEASSARVVGCDIDRESIDWMQANLAPIE
                     AVCSEVDPPLPFESGSFDFIWAISVFTHLTGNSAAWLMELHRLLKPGGLLMASYMGEL
                     NSEEVAGEPWDENRIGMNVLRHSQGWDVGGPMVLMSDWWVDEHWGRAFEIRKRHFAGG
                     QTWALLEKKDVRLSADELMAAGDDPREIAALRHNVAQLQAEIDDLKSHQQGGSLARAG
                     SALKRRASRLRRPD"
     gene            complement(10873..11355)
                     /locus_tag="PLJ64_03245"
     CDS             complement(10873..11355)
                     /locus_tag="PLJ64_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92541.1"
                     /translation="MKTRLTALVLSLATLFSGVLMPVSASAAGWGRGTFTGKVRGQFR
                     PQPLTSVAIAARGDKVRIVKLGIAFDCSDNNQFGLPLHWKTLVYSKAVRVKEGPAGGG
                     AIIHFRVVKNYRGKKVPVRGDIFLGLRDTYIRGTVDSEVARGWNPQDCSDSGIFIARR
                     "
     gene            complement(11840..13357)
                     /locus_tag="PLJ64_03250"
     CDS             complement(11840..13357)
                     /locus_tag="PLJ64_03250"
                     /inference="COORDINATES: protein motif:HMM:NF014307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HNC92542.1"
                     /translation="MSPLLAALAPDSSLVAILYIVSFSLFIIGIKQGTHPTTARRGNM
                     IAAVGMAIAVITTLCLKGIGNWGIIIAGILVGTIVGAVASKRVKMTEMPQMVALYNGL
                     GGGAIALIAWSEYRHWVHLGDVLADKASFSHKFLLDFDNLTFLVGKNFSVSASVPLEV
                     MIPTLLAMVVGSISFWGSNVAFAKLQELIPGQPMGLPGQKIINGLLLAALAVGGVILA
                     INNDVNDLSQPLFIAMLVVAALLGLGVVMPIGGADMPVVISLLNAFTGLSAAAAGIAL
                     DNTALIVAGTLVGSSGTILTLEMATAMNRSVANILFSGFGSTSAGGGGDQEQRPYRSM
                     NAQDAAIQLAYADSVVIVPGYGLAVAQAQHTVKEMADELQKKGVTVNYAIHPVAGRMP
                     GHMNVLLAEADVPYDQLKEMDEINPELPQTDVCVVIGANDVTNPAAKTDESSPIYGMP
                     IIEVDEAQQVLVLKRSMSAGFAGIDNDLFYNEKTAMVFGDAKSTVQDIVTELGNL"
     gene            complement(13354..13701)
                     /locus_tag="PLJ64_03255"
     CDS             complement(13354..13701)
                     /locus_tag="PLJ64_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HNC92543.1"
                     /translation="MNLVTEITILVLAVFVGFEVISKVPTTLHTPLMSQTNAIHGIVL
                     LGGLIVVGYSDDFLGQLIGFIAIVFGTINIVGGFMVTDRMLEMFGPKKKKPEPAAEPA
                     ATDSASDSTEAGS"
     gene            complement(13701..14819)
                     /locus_tag="PLJ64_03260"
     CDS             complement(13701..14819)
                     /locus_tag="PLJ64_03260"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HNC92544.1"
                     /translation="MKIGVPRESAEGENRVALVPESVKSLLKKEGVEIVIESGAGDVA
                     GHPDSQYEEAGAKVGSHADALAADLVLHVAPLSSAQIGELKQGQYLISHLSPLTSAET
                     NNALASAGVTALAMELIPRTTRAQAMDALSSQATAAGYAATLIAARESGSFWGMLTTA
                     AGTIRPARVLVLGAGVAGLQAIATAKRLGAIVTGFDIRRAAWEQIASLGGRPLELDFI
                     PDAEDEGGYARPLTDEENQQVRDAIDANAAKQDVIITTAAIPGRAAPTLITAAGVANM
                     GPGSVILDLAAETGGNCELTEPGQTVVKDGVKIVGPKNLASELPGHASQLYAKNLENL
                     LGLMITEEGGLTLNFEDDIIDAATITHQGEIRGERTAR"
     gene            complement(14907..15956)
                     /locus_tag="PLJ64_03265"
     CDS             complement(14907..15956)
                     /locus_tag="PLJ64_03265"
                     /inference="COORDINATES: protein motif:HMM:TIGR03559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HNC92545.1"
                     /translation="MSLKLGLYLGYWGIGPRGKDAIEAVQFAESIGYESVWVAESYGS
                     DCVSVLAWLAAQTEKINLGAAIMQVPARPPAAAAMAGATIDYLSNGRFMFGFGPSGPQ
                     VSEGWYGVPYSKPWGRTKEYVEVVREIVAREEKLDHQGEHYQLPLPEGQGKPLKLNFK
                     PLRNDIPIFIGAIGRRSVEIAAEVADGWIPIFFSPEKFEEAWGEHIEKGLAAGGRTRD
                     DFEISPSVQVAIDGDLEAARNMVRMALVLYLGGMGSKKTNFYVDLSHRFGFGEVADEV
                     QELFQAGDKEGAFKALPDEFVNAVSLIGTEAEVADRLTAFREKGIDRLIMTPVHPELD
                     QMRHTVERLAALTQD"
     gene            16026..>17058
                     /locus_tag="PLJ64_03270"
     CDS             16026..>17058
                     /locus_tag="PLJ64_03270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNC92546.1"
                     /translation="MTEAATQVLPADDPGRGLSGSEAAERLRSLGPAQPHSSRSTSSI
                     VAGNVFTLFNLIIGIFFVLILSLGLIADAIFGMIAIVNTWIGIRQEMKAKETLDRLAI
                     LVAPRAEVIRDGQALELLADEVVPGDLVRISPGDQLVADGRMTSSRGLTVDESMLTGE
                     SDAIGKSPGDEVFSGSFVISGSGFCEVTAVREESYAGKIAGEAKAFRHPPSPLQEEVN
                     QVILVCTWVLLPLAALLIASLIVRQVGLVDAAQTATAGLITLIPEGLVLLMSVTFAVA
                     AVKMARRDTLVQQMSATESLASVDTICVDKTGTLTDGDLTVVEVIPAKVTDRKLAARE
                     LGRYAASAGD"
BASE COUNT         2843 a         5612 c         5611 g         2992 t
ORIGIN      
        1 tggaggccgc ggtgcgaaca cgatctacgg tggccccgac aacgatgtga tcgacggcgg
       61 cgaggacgga gttgcctggc tattcggggg gccgggagcc gatcggatca ccggcggcag
      121 cgttcttttc gggtgaactt ctctccggag agtcccgctc agtcgggact tccagaaccg
      181 ggtggtgagg atcaggcgct gacggtttgt gaaggctctt cgacttcgag ccagcggatg
      241 ccgtactcgc gcatgtcgtc gatcaggggg acgaggtcct tgcccttgtc ggtcagggcg
      301 taggtgaccc ggggcggaac ctcggggaag gtctggcgtt cgacgatgcc cgcctcttcg
      361 agagtgcgga ggcgcagcga gagggtgcgg gggctgatgc cttcgagcga gcgctcgagt
      421 tcgcagaaac ggcggctttc tccggccagg tcgcggatca cgaggagggt ccacttaccg
      481 gagatcacgt ctgcggtgcg acatacaggg cactgatcgt tgtcgatggc gctcatggtg
      541 tttcggtgac cgtgtcggtt gcggccgggc atccgctcgg gccggttgag tcacttcaac
      601 aagtataggc atatggcttc acttactgaa gcgcaccgcc ggtagcggca agcatgattc
      661 ccgcgcccgc cgccagaaac ggggccagcg gaagggccgt ccgggatggc gggaggcgcc
      721 tgccgagggc aatcaggacc ccggtcaggc cggccagggc gagaccgatg acgagagcga
      781 tccagacctg ctggcccagg tagaggccga ctaccgccac cagcttgacg tcacccatgc
      841 ccatgccttc gggccggatc aggttcgcga gcaggaacgg tgcggccgcc aacaggccga
      901 acaggaccgc ggagccggcc cggccctcgg cggccgccag tccgatcccg gccagggccg
      961 ccccggccat cagccagttc gggatctcgc ggctgcgcaa atcggtcgcc gaggcgatca
     1021 ggagcgtgat gacgaggacg ggaacggcca taccccgacc ctgcccgata aaacctcaca
     1081 cgtggctcta caggtgcaac gattgtgcaa cagaaaggcc agcccggagg ctggcctttc
     1141 gcagttgatt cagatcctga ggatcaagtg gcctcacgaa gcttctgcgc ttcattcagc
     1201 gactggagct tcttcagcga ctgattctcg atctgacgga tccgctcgcg ggtcacgttg
     1261 aaggtgcgcc cgacctcgtc gagggtgcgg gggtgctcgc cgccgagccc gtaacggagc
     1321 tcgagcacgc ggcgctcgcg gtaggagagg ttctccagcg attccttcag cgcttccttg
     1381 gtcaacaggt cggctgcccg gtcaaagggc gactcggcct tctcgtcggc aatgaactgg
     1441 ccgaactcgg attcctcctc gtcaccgacc ggcttctcga gcgagacggg tgactgggcg
     1501 gaacgcttga tcgagtcgac ttcctcgggg tcgatgccgg ttacttcggc gatctcctcc
     1561 ggggtgggct cgcggcccag ttccgtcacc agcttgcgct cggcacggcc gatcttgttc
     1621 agcttctcga caacgtggac cgggatccgg atggtccggg ccttgtcggc gagggcgcgg
     1681 gcgatcgcct ggcggatcca ccaggtcgcg taggtcgaga acttgaatcc cttgcggtaa
     1741 tcgaacttct cggccgcacg gaccaggccg agcgtgcctt cctggatcaa atcgaggaaa
     1801 ggcaggccct ggttgcggta gttcttggcg atcgagacga cgagccggag gttcgactcg
     1861 accatcttct gcttggcgtc gaggtcaccg cgctcgatcc gcttggcgag atcgacttct
     1921 tcctgggcgg tcagcaggcg aaccttgccg atgtccttga ggaagagctg cagtgagtcg
     1981 gtggtcatgt ccggcttgag gtcgagcgcc tccttcttgc gccggcggcc gcggcggccg
     2041 tcggagcgct cgttctcggc cgaagccttc tgcgccggat ccacatcctc gaccagctcg
     2101 atcccctgct tttcgatgtg accgtaaagg tcttcgacgt ctgattcgtc gagatcgacc
     2161 tcagccacgg cagtggcaac gtcaccgaaa gtgaggacgc cgagctgctg acccctgttg
     2221 acgagggtct ttacttcttc tagttcctgg agttcggcta caagcatcat gtccctatct
     2281 ggtattccgt aagacggcgt ctatacctgt ctacccgaaa atgagggtcc gggtcacaga
     2341 aaatcttgcg aatcactcgt caggatccgc atcgctccat atgtaaaccg gagcaggaaa
     2401 aaccgccgcc gtctgtaagt ggtcaaacga gtgaccggat aatagccact tcacttagta
     2461 aagtcaaggg ctgcgatccg tgaactctgc ttcactcaag gaacgcgtac cggccccatg
     2521 agttgcactg actgtgacga aaatcatccg gagcatccca acgatgcccc gcttcccctc
     2581 tccgagcaat ggaagcacct ccactggctg caggccttct ggacccgggc ctggcgggaa
     2641 aacgtaaccg ggctctccgg catggtcgcc tacaacctga tgctggcgat cttcccgttc
     2701 gcgttcctcg tgctgttcat cttctcccag gtactgcggg tcgaaggaat cgaccagggc
     2761 atcatcaacg accttcagcg gctcttcccg aacgccgagc agggcacgct cgacgacatc
     2821 ctcaaggcga tccgcaacaa ctccgccacc ctcggtgtgg ccgccgtctt cggctcgctc
     2881 tggatcggcg cctcgttctg gggggcgatg gacaccgcct tctgccggat ctatcacgtg
     2941 cagtgccgcg gctggtggga gcagaagcga ttctcgctcg tcatgttgct ggtggtcacg
     3001 atcttcctgc tcggcagcgt cctcctcccc gccctcgagg gtgccctgat ccgtcagacc
     3061 gacaaccttc ccttcggcct gaatgaggtc cagaacctgg acaacgcggt attgctcgcg
     3121 gtcacactca gcctgaactt cacggtcgcg gcagtgatct actgggcggt gccgaagggc
     3181 cacatgccct ggcgggcggt ctggccggga gccctcttca cgaccctggt cgccgccgct
     3241 gcgaactggc tcttcccggt ctacctcaac aacgtctcga ccctgaaccg tttcggctcg
     3301 acgatcggct tcatcctgat cgcccttttc tggttttacc tggtcgccct gaccatgctg
     3361 gccggcgcgg tgatcaactc gatccgccac gagtaccacg acaccgggag cctgccctac
     3421 ggcatcctca gccgggccca gctgctggcc agggcccagg ccgacgaggc gaaggccgca
     3481 cagcgaccga tggccgagcc ctaccgctcg ctggtcagcg gtgacgcctg gccgaagccg
     3541 gaggccggcc cggacgaaga gagcgaggcc gagaccaagg tcattccgct gcgccgtcgc
     3601 aagcgccaat gagcgtcagg atcgagctga tgccggaccc ggtccggcac ggactggccc
     3661 ggaccggcgg cgtgacctgc gagcaggagg cggtgatcac ggtcgaccgc gaaaccttcg
     3721 agcagatctg gacgccttcc accctggaat tactggccag gtcctactgg gattacattc
     3781 gccgtttcac gctgggggcg attcgggtcc agtaccgggt ggactcccag accgtgacgc
     3841 tgctgggccg ggtgccgctg ctccgcttcc gcaccccggt cttttcgacc ggcaacgacc
     3901 gggcctcgat cgagtggccg gtcgaaaagg gactgctggt cgcccgcgac ggccgcggca
     3961 agggttttct gcggattgag gcttcccgcg gccaggccga ggacgccggg tgcccgaccc
     4021 tgatggtctc ttcgacggtt tcgaacttct atccctggtt gagggggacc ggccggttcg
     4081 ccaggttcgg aacctggctt tactcgcaga cccagctgcg catccacatc ctcgtgacca
     4141 agggcttcct ccgctcgctc gacgagctgc cggacgaggt gctccggcgg ggcgtatcgc
     4201 cgggaaccgg ttagccgccg gcttcgattt ccgcgacggc cttccgaagg accgttccga
     4261 gcgattcggg ctgaaccccc agctcttccg ggcccggcgg ctgcgtcacc accgtttcgg
     4321 tcgaaagccc ctcgatcagc ggccgggcga ccccgacgtc aaccggcgtg atcagtccga
     4381 gccagagcga agacagcctc ggggtgagaa agggcacacc gatccggagc ggtggccttc
     4441 tccccatcgc tttcgcggtc tcgtccatca gtccgccgta ggtcgtcacg tccggcccgc
     4501 caatctggat ctcgctgttg ccgtccagct tcagcgcccg cgccaggcac tcgaccacgt
     4561 cgtcgatgcc gatcggctgg gtgcgaaccg aagtccagcg gggcgtgacc atcgccggca
     4621 ggcggcgaac cagccagtag atcgtctgga acgactcgct accggcaccg atcaccacgg
     4681 cggcccgcat gtaggtgacg tcgatcccgg tcgagcccaa cgcctgcgcg gtctcgtggc
     4741 gggaccgcag gtgccgggag cgaccctccc ccaggccgcc caggtaaacg attcgctcga
     4801 cgccgacctc ggccgccgct tcggcgaagt tgcttgcccc cttcagatcc gattcggcga
     4861 agtcaccgct cgccccgcgg cccatcgagt gcaccaggta gtaggcgacc cggactccgg
     4921 aaagtggcca gcggagcgat tcccggtcaa gcacgtcgcc ggccaccact tcacagccgg
     4981 cttcttccag gtcccccgcc cgggccgggt tccgggccat gcagcgaacg gcgttgccgc
     5041 cccggaccag cctcagggcc agcctgcggc cgatgtagcc ggtcgaaccg gcgacaagta
     5101 ccggctcagc cgaagaggcc accagtcact tcacgaagct tggcgcggtc gtggcgggcg
     5161 ccgatcttgc ggacggtgcc ggaacgggaa cgcatgacca gagactcggt gctcgcccag
     5221 cccttgtggc ggcggacgcc gtcgagcagg tcaccgtcag tgacgccggt cgcggcaaag
     5281 aagacatcgt ccccggagac caggtcgtcg gcggtcaaaa cccggtcgag gtcgtagccg
     5341 gcgtcgattg ccgcctggcg ttcctcgtcg tttcggggcc agagcttgcc gatgatctcc
     5401 ccgccgaggc acttgatcgc agccgcggcg agcacgcctt cgggggtgcc gccgatgccc
     5461 cagagcaggt cgatgccggt gtcgtccgag accgccagca gggcggcctg cacgtcgccg
     5521 tcggtgatga agcggatcgt cgcgccggac tcccggactt ccttgaccac ttcctcgtgg
     5581 cgaggccggt cgagaatggt cagggtgacg tcttcgggtc cgccgccctt gcgctcgccg
     5641 atcttgcgga tcacttcgcc gatcggctcg tcgaaggtga gcaggtcctt gaactcggac
     5701 gcggcggcga gcttctccat gtagacacag ggcccggggt cgaacatcgt gccgcgctcg
     5761 gagagggcga tcaccgcgag ggcgccgccg aaaccgcggg cggtcagggt cgtgccttcg
     5821 agcggatcga cggcgatgtc aaccacgggc gggctgccgt ccccgatctt ctcgccgttg
     5881 tagagcatcg gcgcttcgtc cttctcgcct tcaccgatca ccacgatccc gtccatcggg
     5941 acggtgtcga gcaggatgcg catcgcgtcg acggcggcgc cgtcggcggc aagcttgtca
     6001 ccccgcccga tccaccgcga ggcggcgagg gccgccgact cggtgacgcg aaccagctcc
     6061 agggccaggt tgcggtcggg ctttgatccg gatcgaacgt cgtgcgagat aacacccatg
     6121 cggggcaggc tatataggct gccgccatga gcgcacgctg gggcttgacc ctccccctgc
     6181 ccggggtctc actggccgac accaaggact tcgttcagct ggcggaggcc gagggttaca
     6241 ccgacctctg gaccggggaa accggcggcc cggatggctt cacgccgctc gccctgagcg
     6301 ccgcctggac cagcaaggtg cggctcggca ccggcatcgt cggggtcttc cagcgcggac
     6361 cggcgctgct cgcccagcag gccgccgccc tgaccgacgc ttcctccggc cgcttcgccc
     6421 tcggcatcgg cgcctcctcc gaccgcatgg tcgagggctg gaaccagatc cccttcgtca
     6481 agccgctttc caaggtcgag caaaccgtgg acttcctgcg ggtagccctc gccggggagc
     6541 gcaccgacac cggcttcaag ctggagacgg ccccgaccca cgaaaccccg atcgtgctgg
     6601 ccgccctgcg cggcaagatg ctcgagctgg cggtcaacaa ggccgacggc gccttcacca
     6661 acttcctgcc cctggccggc ctgccgaagg tggtcgacgg catcgagggc gccggcaacg
     6721 acttcgagct gctttgccgt ttcttctgca tccccggtga aaaggaacag gtcgagggcc
     6781 tcgcccggtt catgttctcc agctacatca cggtgccggt ttacgcgaac ttcttccgct
     6841 ggctcggcta cggcgaagag atcgaagcga tggtcgagca ctggaacaac aaggaccgcc
     6901 aggccgccgc ggcggccgcg ccctgggagc tgatcgaaga caccttcctg ctcggcagcc
     6961 cggagcagat ccgcgcgagg ctcgatgaat acgtcgccgg cgggatcacc ctgcccgtga
     7021 tcaccccggt caccaccccg gacaatcacg ccgcgatgat caaggccctg gctccctgaa
     7081 gccttcctcc aatcgagaag gcgaaagtgg ccatatagcg ctccattcag ccttctcggc
     7141 tgctcccgac atgcgggcag ctcgaaagcc agccggctag ccggttccct cggcttcggg
     7201 ttcggtccag acctccttct cccagggcgg cgtgatcgga tcgaagtcgg ccggtcgtgc
     7261 gagcggaccg tccttgaccg attccagcgg gaccagccgg accagggcgg acggagtgta
     7321 ggaggggtag tccgaatcgg cgaggctggc ggcaacgttg gcggcgtggc tgccgccggc
     7381 gatcaggtac tgcccggtcg catcacccca gcctcccttc tcgcgcaggc caaccttctc
     7441 tgccgcgtcg gtcagaaagc cccagccgag gtcggagccc cagttgaccg acgaccgcgt
     7501 gtggttgtag ccggcatggg aggaagcccg ctcgctgacc actccgtcgg gatcgaccct
     7561 gatctgcatt gattcccagt cattccggtg gtagccctcg tcctcgagga gcggtgcccc
     7621 tcgaagcgag gcgctctcgg ggtagtaggc ccagtactgg atgtagatgc tgccgtcggg
     7681 ctcgtggatg accctggtga aaagcgttac cggctctccg gcagtggacc ggctgacctc
     7741 gccctccccg gttccgtcgg cgcaccaggg cgatcggcag gtccggtaat cgaccggcag
     7801 gccgagcatg tcctttccgt acataagggc cggcgccccg gaacgaacga gttcagcgac
     7861 ttccggaccg taggcctcgt cgagcgaacc ctcgccggcg caggaaccgg acaacgaggc
     7921 ggcgcagagc aacctggtcg agacggaatg ggcaagcccg accgccggca cctggattcc
     7981 cgccgcggtg agagccgcca tcagggccgc gaccagggtg atcaacccga cccactcgat
     8041 cgtcgattgg ccccgcgtcg atttcagcct cacgcccatc gcccaatcgt ggcgggaaaa
     8101 agctcacaag tgtgttaccg gtgcaacgat tcagtgacgg acggccgagg taaaatcggt
     8161 ccatggccga tcgcaacaag atgaccccgc cgtccagtgc ttcgaagaag acccccgaat
     8221 ggaagtggtg ggccttcggc atcgcagtcc tcctgctgct gatcgtggtt ctgcagaact
     8281 cccaggacgt ggagttcaag attctcttcc ttgtcgacac cgcggcgccg ctcttcgtgc
     8341 tgctgctgtt cgccgcggcg atcggcgcga tcatcggcta caccggcccg atcctccgcc
     8401 gccaccgcca caacacccgc aaggaatacg gcaaggactg agcggcgcga tgtcgcttcc
     8461 gatgatcgaa ccggtccgcc tcgaaggcga ccgggtggca ctggagccga tccgtgccga
     8521 tctcgccgac gatctctgga ccgccgcgca ggacccggag atctggcact ggaccaccca
     8581 tctgaaccac agtcgcgagt tctttgacgg ctgggttgcg ggcggcatcg aaggctggga
     8641 gagcggcgag cgcagcacct ttgtgaccag cctccggtcc gcgggaagcg acgagccagc
     8701 ccgggttgtc ggcagcagca gctacctctt ctaccggccg gacgaggacg tggtcgagat
     8761 cggctcgacc tggctgaatc ccggggcctg gggcacgggc gccaacaccg aagcgaagct
     8821 gctcatgatg acccacgctt tcgagacgct cggctgcgtt cgggtcgagt tcaagaccga
     8881 ccgtcgcaac gagcgctcgc gagcggcact cgcggctttg ccggcgcagt tcgaggggat
     8941 cctgcgcaag cacacgaatc cgttcggggt cggccgccga gattccgcct actacagcgt
     9001 gatcgatgac gagtggcccg aagttcgcgc ggcgctcgag gcccgcctgg cccgccgatc
     9061 ctgagccccg gccccggggc gccgcttcag tcctgcccga tccgctttcc cgcgatctcg
     9121 accgcttccg ggttctcgtc gatcagcagg tagtcccggc ccagtttccg gcagaccgcg
     9181 ccgagcgttc ccgaaccgca gaaggggtcg aggcaaaggt cccccggccg ggttgatgcg
     9241 agcaccatcc tgcgtacgat cccttccggt ttctgggtcg ggtagccggt cttctcggcc
     9301 ccgttggtcg gcacgatcgt gtgccaccag acgtcggtcg gcagtttgcc cttggccacc
     9361 ttctcggggg tgaccagccc cggcgccatg tacggctccc ggtccacctc gccggagtcg
     9421 aagtagtagc ggtccggatc cttgacatag accaggatcg tgtcgtgctt ggccggccag
     9481 cgccgcttcg acttgccgcc gtagtcgtag gcccagatga tctcgttgag gaacgactcc
     9541 cggccgaaga tgccgtccag catcactttc acgtaatggg cttcgcggta gtcgatatgc
     9601 aggtagagca ttcctgtttc gttcagcagg cggtgaatct gcttcagccg cggctccagg
     9661 aaggcggtga agtcatcgaa ggcatccgag tacgaggcct ctcccgcctt ttccgtgcgg
     9721 tagcgccggc cgccgaagcc ggtccggtcc ccatccgtgt cggcaacggt ccgcagggcc
     9781 tgccgcgact gctgtttgcc ggtgttgaac ggcgggtcgg catagaccat ctggaaggac
     9841 ccggccgcca gtccggccat caggtcgagg ttgtcgccga gcaggatccg gttgcgctcc
     9901 attccggaaa cctagatgtt gcagcccggt caacgggagc ccggttgcct ccgggaacgg
     9961 gcgatcccgt agtttccccg aggtgaccgc gagcagcgac ctcccctatc cgccctttga
    10021 gctcgccaac cgcgtaatcg agttgcccag caacgacttc gccgggtacg tccactacga
    10081 aatggccggc cggcagacgc gggatgagct gctggatctg ctgcccgacg gatacacact
    10141 ggaaggtcgc agcctgctcg acttcggctg cggcgccggc cgcaccctgc ggcaattcca
    10201 cgacgaagct tcctcggccc gggtggtcgg atgcgacatc gaccgggaaa gcatcgactg
    10261 gatgcaggcg aacctggcgc cgatcgaggc ggtctgctcc gaagtcgatc cgcccctgcc
    10321 cttcgagtcg ggaagcttcg acttcatctg ggcaatttcg gtgttcaccc acctgaccgg
    10381 caactcggcc gcctggctga tggaactcca ccgcctgctc aagccgggcg gtctgctcat
    10441 ggcctcctac atgggcgagc tcaactcgga agaggtcgcc ggcgagccgt gggacgagaa
    10501 ccggatcggc atgaacgtgc ttcgtcacag ccagggatgg gatgtgggcg gtccgatggt
    10561 cttgatgtcc gactggtggg ttgacgagca ctggggccgg gccttcgaga tccgcaaacg
    10621 gcacttcgcg ggaggtcaga cctgggccct tctggaaaag aaggatgtcc ggctttccgc
    10681 cgatgaactg atggcggccg gcgacgatcc ccgtgagatc gccgccctcc gccacaacgt
    10741 cgcccagctc caggcggaga tcgacgacct caagtcccat cagcagggcg ggtcactcgc
    10801 ccgggccggt tcagccctga agcgaagggc cagccgtttg cgtcgcccgg actgagccgg
    10861 cgccggggcg tcctacctgc gggcgatgaa gatgccgctg tccgagcagt cctggggatt
    10921 ccagccgcgg gcaacctctg aatcgaccgt gccccggatg taggtatcgc gcaggccgag
    10981 gaagatgtcg cccctgaccg ggactttctt gccccggtag ttcttgacca cccggaagtg
    11041 gatgatcgcc ccgcctccgg ccggcccttc ctttacccgc accgccttgc tgtagacgag
    11101 tgtcttccag tgaagcggca ggccgaactg attgttgtcc gagcagtcga aggcgatccc
    11161 gagcttgacg atccgcacct tgtcaccccg cgcggcaatg gccacgctgg tcagcggctg
    11221 aggccggaac tgaccccgca ccttgccggt gaaggtgccg cggccccagc cggctgcgga
    11281 agccgaaacg ggcatcagga cgccggaaaa gagggtggcc agggacagga cgagggctgt
    11341 gagtcgggtc ttcatgctcc caacctagaa accccacctt gccaccacct tgcgaacccg
    11401 gcttcgcaag tctcgtgggt cggatttccg gcccgattgc cggttatccg acccactgcc
    11461 gataaacagc ggcattgaag ggggagttga gggtgttccc gagtcctgtg gcacctccgg
    11521 ggcccggttc aggcccgggt ttcgggaggt gggtatggat ttcgtctata tggacgaaat
    11581 ccatacccac ctcccccgga tcgggtcagg ttgcgatcga gatcggaatt gggtgcgcta
    11641 tccgccgaaa aaccagatct cgaggatgcc aacaccactt ggcttgagcg ggtcgtgcac
    11701 agggcgctcc tgcgggtggc tggcgaggaa taagccggga aatggccgcg atcgtacgcg
    11761 ggtacttgag ctgccgtttc ccggcgttga cgaagcccag gcgcccgcag gtgcgtcctg
    11821 ggcgcgttgc ccggggggtt tacaggttgc cgagctctgt gactatgtcc tggacggtgc
    11881 tcttggcgtc gccgaagacc atggcggtct tctcgttgta gaagagatcg ttgtcgatcc
    11941 cggcgaaacc ggcggacatc gagcgcttga ggaccagcac ctgctgggct tcgtcgacct
    12001 cgatgatcgg catgccgtag atcgggctgg actcgtcggt cttggccgcc gggttggtga
    12061 catcgttcgc gccaatcacc acgcagacgt cggtctgcgg aagctcgggg ttgatctcgt
    12121 ccatttcctt gagctggtcg tagggcacgt cggcctcggc cagcaggacg ttcatgtgac
    12181 cgggcatgcg gccggcgacc gggtggatcg cgtagttgac cgttacgccc ttcttctgga
    12241 gctcgtcggc catttccttg accgtgtgct gggcctgggc gacggcaaga ccgtaaccgg
    12301 gcacgatcac gaccgagtcc gcgtaggcga gctggatcgc ggcgtcctgg gcgttcatcg
    12361 aacggtatgg ccgctgttcc tggtccccgc caccaccggc cgaggtcgaa ccgaatccgg
    12421 agaagaggat gttggcgacg gaccggttca ttgcggtcgc catttccagg gttaggatcg
    12481 tgcccgagga accgaccagg gtgccggcca cgatcagggc ggtgttgtcg agggcgatgc
    12541 cggcggccgc cgcggatagg ccggtgaagg cattgagcag cgagatcacg accggcatgt
    12601 cggccccgcc gatcggcatg accacgccga ggccgagcag ggcggccacg accagcatgg
    12661 cgatgaagag cggctggctc aggtcgttga cgtcgttgtt gatggcgagg atgaccccgc
    12721 cgaccgcgag cgcggcgagc aacaggccgt tgatgatctt ctggcctggc aggcccatcg
    12781 gctggcccgg gatcagttcc tgcagcttgg cgaaggcgac gttggagccc cagaaggaaa
    12841 tcgagccgac aaccatggcg agcagggtcg ggatcatcac ttcgagcggc accgaagcgc
    12901 tgacgctgaa gttcttcccg acgaggaagg tgaggttgtc gaaatcgagc aggaacttgt
    12961 ggctgaagct ggccttgtcg gcgagcacgt caccgaggtg gacccagtgc cggtactcgg
    13021 accatgcgat cagggcgatc gcgccgccgc cgaggccgtt gtagagcgcg accatctgcg
    13081 gcatttcggt catcttgacc cgcttcgagg cgaccgcgcc gacgatcgtg ccgaccagga
    13141 tgccggcgat gatgatgccc cagttgccga tccccttcag gcagagcgtg gtgatgaccg
    13201 cgatcgccat accgacggcg gcaatcatgt tgccgcgccg ggcggtggtc gggtgggtgc
    13261 cctgcttgat cccgatgatg aacagcgaga agctgacgat gtagagaatc gcgaccagcg
    13321 acgaatccgg cgccagcgca gcgagaagcg gactcacgag ccggcctccg tcgagtccga
    13381 cgcagaatcg gtagctgccg gttcagccgc gggttcgggc tttttcttct tcggcccgaa
    13441 catttccagc atgcggtcgg tgaccatgaa gccgccgacg atgttgatcg tgccgaagac
    13501 gatcgcgatg aagccgatca gctggccgag gaagtcatcg gagtagccga ccacgatcag
    13561 gccgcccagc aggacgatcc cgtggatcgc gttggtctgc gacatcagcg gcgtgtgcag
    13621 cgtggtcgga accttggaga tcacttcgaa gccgacgaag accgccagca cgaggatggt
    13681 tatctcggtt acgaggttca tcagcgggcg gtcctctctc cgcgaatttc accctggtgg
    13741 gtgatcgtcg cggcatcgat gatgtcgtcc tcgaagttca gggtgagacc gccctcctcg
    13801 gtgatcatca gtccgagcag gttctcgagg ttcttggcgt aaagctggga tgcgtgaccg
    13861 ggcagctccg aagcgaggtt cttcgggccg acgatcttga ctccgtcctt gaccacggtc
    13921 tggccgggct cggtcagctc gcagttgccg ccggtctcgg cggccaggtc gaggatcacc
    13981 gatccgggac ccatgttggc gacaccggcc gcggtgatca gggtcggcgc ggcgcgaccg
    14041 gggatcgcgg cggtggtgat gatcacgtcc tgcttggccg cgttcgcgtc gatcgcgtcg
    14101 cggacctgct ggttttcctc gtcggtcagg ggacgggcgt agccgccctc gtcctcggcg
    14161 tcgggaatga agtcaagctc gagcgggcga ccgcccaggg aggcgatctg ttcccaggcg
    14221 gcgcggcgga tgtcgaaacc ggtgacgatc gccccgagac gcttggccgt ggcgatcgcc
    14281 tgcagcccgg cgacgccggc cccgagcacc aggacgcggg ccggacggat cgtgccggca
    14341 gcggtggtga gcatgcccca gaagctgccc gactcgcgcg cggcgatcag agtcgcggca
    14401 taaccggccg cggtcgcctg agaggaaagt gcgtccatcg cctgggcgcg ggtggtgcgc
    14461 ggaatcagtt ccatcgccag ggcggtgacc ccggccgagg cgagggcgtt gttggtctcc
    14521 gccgaggtca gcggcgagag atggctgatc aggtactggc cctgcttcaa ctcgccgatc
    14581 tgggcgctcg aaagcggagc gacgtgaagc acgagatcgg ccgcgagcgc gtcggcatgc
    14641 gaaccgacct tcgctcccgc ttcctcgtac tggctgtctg gatgcccggc gacatcgccg
    14701 gctccggact cgatgacgat ctcgacgcct tcctttttca ggagcgattt gaccgactcg
    14761 ggaaccaggg caacccggtt ctcgccttca gcagattctc tgggtacacc gatcttcatg
    14821 gacaaggccg actcagatta tgggaattag ccgcagaggg gattcccgac cggcggacag
    14881 ccgccggccg gatcagatcc tcttggtcag tcctgcgtca gggcggccag ccgctcgacg
    14941 gtgtgccgca tctgatcgag ctccgggtgg accggggtca tgatcagccg gtcgatcccc
    15001 ttttcgcgga aggcggtcag ccggtcggcc acttcggcct cggtcccgat cagcgagacc
    15061 gcgttgacga actcgtcggg cagcgccttg aacgcgcctt ccttgtcgcc ggcctggaag
    15121 agctcctgaa cctcgtcggc gacttcaccg aagccgaaac ggtgggagag gtcaacgtag
    15181 aagttggtct tcttcgatcc catgccgccg aggtagagga ccagagccat gcgaaccatg
    15241 ttccgggccg cctcgaggtc accgtcgatc gcgacctgga cggaaggcga gatttcgaag
    15301 tcatcccgcg tgcgcccgcc ggcggcgagc cccttttcga tgtgctcgcc ccaggcttcc
    15361 tcgaacttct ccggactgaa gaagatcggg atccagccgt cggcgacctc ggccgcgatt
    15421 tcgaccgaac ggcggccgat cgcaccgatg aagatcggga tgtcgttgcg cagcggcttg
    15481 aagttgagct tcaagggctt gccctggcct tcgggcaggg gcagctggta gtgctcgccc
    15541 tgatggtcga gcttctcctc acgggcgacg atctcgcgga ccacctcgac gtattccttg
    15601 gtccggcccc agggcttgga gtagggaacg ccgtaccagc cctcggaaac ctgcgggccc
    15661 gaggggccga agccgaacat gaagcggccg ttcgagaggt agtcgatcgt ggcgccggcc
    15721 atcgcggcgg cagccggcgg gcgggccggg acctgcatga tcgccgcgcc gagattgatc
    15781 ttctcggtct gggcggcaag ccaggcgagg acgctgacgc agtccgatcc atacgactcg
    15841 gccacccaga ccgattcgta gccgatgctt tcggcgaact gcaccgcctc gatcgcatct
    15901 tttccgcggg gcccgatgcc ccaataaccg agatacagcc caagcttcag tgacatgcgg
    15961 gcatcctacc cgaaagtaca aattctgttt ttgttccaac cgggcaaacc ggtaacaatc
    16021 attccgtgac tgaagcggcc acccaggtac tcccagccga tgatccgggc cgcggcctgt
    16081 ccggttccga agcggccgag cgcctgcgca gcctcgggcc cgcccagccc cactcctcgc
    16141 ggtcgacctc cagcatcgtc gccggcaacg tcttcacgct cttcaacctg atcatcggga
    16201 tcttcttcgt cctgatcctc tcgctcgggc tgatcgccga tgcgatcttc ggaatgatcg
    16261 cgatcgtcaa cacctggatc ggcattcgcc aggagatgaa ggcgaaggag accctggacc
    16321 ggctggcgat cctggtcgcc ccgcgggccg aggtgatccg cgatggccag gcgctggagc
    16381 tgctggccga cgaggtggtg cccggtgacc tggtccggat ttcccccggg gaccagctgg
    16441 tcgccgacgg ccggatgacc agctcccgcg gcctgaccgt cgacgaatcg atgctgaccg
    16501 gcgagtccga tgcgatcggc aagtccccgg gcgacgaggt cttctccggc agcttcgtga
    16561 tctccggttc gggcttttgc gaagtgaccg cggttcggga ggagagctat gccgggaaga
    16621 tcgccggcga ggcgaaggcc ttccgccacc cgccttcacc gctccaggaa gaggtcaacc
    16681 aggtgatcct ggtctgcaca tgggtcctgt tgccgctggc cgcgctcctg atcgcgtcgc
    16741 tgatagtccg ccaggtcggg ctggtcgacg ccgcccagac cgcgaccgcc ggcctgatca
    16801 ccctgatccc cgagggcctg gtcctgctga tgagcgtgac cttcgccgtc gcggccgtga
    16861 agatggcccg gcgggacact ctggtccagc agatgagcgc gaccgaatca cttgcttcgg
    16921 ttgacacgat ctgcgtcgac aagacgggca ccctgaccga cggcgacctg accgtggtcg
    16981 aggtgattcc ggccaaggtc accgaccgca agctcgccgc ccgcgagctc ggccgctacg
    17041 ccgccagtgc cggtgacc
//
LOCUS       DAOQKN010000028        17054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_1525_length_17054_cov_4.626037, whole genome shotgun sequence.
ACCESSION   DAOQKN010000028 DAOQKN010000000
VERSION     DAOQKN010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 17054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17054
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1525_length_17054_cov_4.626037"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..503)
                     /locus_tag="PLJ64_03275"
     CDS             complement(174..503)
                     /locus_tag="PLJ64_03275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC92547.1"
                     /translation="MAGAAVAHRPGADPERNGPNGSLSLSVPAELVELVAARAAELLA
                     HRMTEPAADGYLDVKGAAEFLSCPVSRIYALVSARRIPFHKDGSRTLFDRGELRQYVE
                     AGGARRP"
     gene            complement(518..1636)
                     /locus_tag="PLJ64_03280"
     CDS             complement(518..1636)
                     /locus_tag="PLJ64_03280"
                     /inference="COORDINATES: protein motif:HMM:NF024872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC92548.1"
                     /translation="MDRPSNAWGGVIPLTPEQEAACLAEVQDSDPPRLLTLDQLADLP
                     EPSYLVEDFIRARGMTVLYGPSGGGKSFAALDWSLCIATGQPFYGQAVQQGPVVYIAA
                     EGASGYYSRAQAWCNSRGVAEVPDFRFWIEAPNFFRSETTALEVALAELDKPPVLIVI
                     DTLARCLVGGDENSARDIGLFIQNAEKLAKRHGSALLIVHHTGKSGELERGSSALRGA
                     ADTMLCLKPDGANLKLTCEKQKDAAEFDPWRFHLEPQNESCVIRLGDDVGRLSDHEQR
                     ILETVSESFGTEWVSATKIKEVSDIPKSSFYRSLNTLVERNLIEERQATKYRKEYRLT
                     QTANPVPSRPKPSHGTTETVPSHPPFSGVGHGTGRGQE"
     gene            complement(1603..2124)
                     /locus_tag="PLJ64_03285"
     CDS             complement(1603..2124)
                     /locus_tag="PLJ64_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92549.1"
                     /translation="MTQPTFQQFQRPLPGLSSHDRLLLFHALPESVQQSMWDRLAIQV
                     EDRRYADGDGPFIDRRPKPKRRPIRTASNLPSDSWLRGTEALASIPAETYLTVLAPES
                     EPTRGKCRCPLPDHEDHHPSATYKDTVWYCHVCGIGGTCFTIAAAKTGLNTRGRDFIE
                     LRKWIARQMLGAA"
     gene            2361..3080
                     /locus_tag="PLJ64_03290"
     CDS             2361..3080
                     /locus_tag="PLJ64_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92550.1"
                     /translation="MDNVTPTTTSSMSCLETGGGWLRNRLVLDVRRERLGGPESASAW
                     IEADHQNGLTYAARIVGVGKYAVIAEAKVFPTQPDRVEARTDFLGRSHGERSRRGGEI
                     VDADASPSPGGLTTGDVRKMPTDALLSMARARLLKIAEVPSPVLDCFPELSVPRREGE
                     RPHRRDDRAVANVAATYAELVESGHRKPLRRTAELHGYAIKGVEKVIAAARERDILTP
                     TTQGKAGGELTNLGRQLAQSQ"
     gene            3139..3840
                     /locus_tag="PLJ64_03295"
     CDS             3139..3840
                     /locus_tag="PLJ64_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92551.1"
                     /translation="MLGRKIAVTICCLIAYAGALSVQGATADVAVPVNISPANNAVLI
                     ADDPSPWVTLPDVALITLKYSCDASKASSYFVEITGGDSTPGLLGTTLSSYNDGVCEA
                     TNSDGYEPGTYTWRPFLITGLGDAGQAYGSKWTFTVEERPSDPDPDPILDTHMNLSQA
                     KFYARAQTKKRFYRAPFRVSCWRKSSSSFGCFSFYRQNWRKRFRAKWVFAHVVDDNQL
                     WYTTSFKGKSRYSGW"
     gene            complement(3928..4001)
                     /locus_tag="PLJ64_03300"
     tRNA            complement(3928..4001)
                     /locus_tag="PLJ64_03300"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3965..3967),aa:Met,seq:cat)
     gene            complement(4073..5104)
                     /locus_tag="PLJ64_03305"
     CDS             complement(4073..5104)
                     /locus_tag="PLJ64_03305"
                     /inference="COORDINATES: protein motif:HMM:NF015552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance/C4-dicarboxylate
                     transporter family protein"
                     /protein_id="HNC92552.1"
                     /translation="MTGDSRLRESIRTLYPGYFALVMATGIISNGFYLLDFKVLSAVM
                     IVIAVGAFLVLATATIARALLFPRELAADLVDPRFVFSFFTLVAGVNVLGIQLFMRGK
                     LIPAIVLWIFSIVVWVPLSYLSFAVVTFRDSRQGGGVMNGGWLIAIVGAESIAALGGK
                     LSTQIGTGAELVFVIAHAFWGIGMILYGIFATLFANRIFFFRVTPEEMNPLFWVVMGA
                     AAITVNAGSAMIIAQSEIGYLADLEPFVEGASLVLWAWATWWIPLLVIFGIWRHVVRK
                     VPLTYSPMYWSLVFPLGMYTVATWQFATADNFSFMKEIPSYTIWIALTAWLVTATGLV
                     RSIFRRPTT"
     gene            complement(5112..6692)
                     /locus_tag="PLJ64_03310"
     CDS             complement(5112..6692)
                     /locus_tag="PLJ64_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC92553.1"
                     /translation="MSGFAGTADAIRFALKRDRLILPIWVAVLIGWIAMEVISYKNLY
                     GTTAQLDALYRSVAGNPAMVAISGPTGGIRTLGGAITWDSLPTISVVSGVFAMFSVVR
                     HTRAEEEDGRTELLLAYGSGRLAPLAAALTVTLAALALASAGYVAVFAAEGLPVGPAT
                     LLSLSFFGFGAVIAGTTAVVCQVTSKARAARGLVGIVIGAAWLLRAIGDTGGGTLTWF
                     SPLGWAEQTRPFWEDRWWPLLLPLAAFAVTLALSGWLLVRRDIGGGLIQPRPGPARAH
                     KSLLHPLGFAFRIQRGSIIGWAVMLFAYGFAVGVIGDNIEQLLKTSSAFTEAFASASG
                     NLLDSYFASILTVIALLASGFTVSAVLRPHSEESRGRVGLLLAAPLGKVRWVANHLLI
                     AFAATAVIIALVGLGLGIGFAVATGDSSQIGRLTGAAVAQTPAMWLTGSLVVLLYGLS
                     SRLSPVAWGLLATFVLIWTLASFGDIPDWIVDLSPFSHTPAVPAVPLDLTPLLVMTAL
                     SAALTAGGLALWRRRDLL"
     gene            complement(6689..7423)
                     /locus_tag="PLJ64_03315"
     CDS             complement(6689..7423)
                     /locus_tag="PLJ64_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC92554.1"
                     /translation="MSAVIEVEGLIKNFGSTRALDGLDLKVEPGQVHGFLGPNGSGKS
                     TTIRILLGLINASGGSARLFGEDPWKNAVSLHRRLAYVPGDVNLWPNLSGGETIDMLG
                     SLRGGLDPGRRKELVERFELDLTKKASTYSKGNRQKVALIAALATDAELFIFDEPTSG
                     LDPLKEAVFQECVKEITGQGHTILLSSHILGQLEHLADTFTIIKDGRTVETGTLAHLR
                     EIAASAGRDPYSLSLEDLFLSIYANP"
     gene            complement(7434..8021)
                     /locus_tag="PLJ64_03320"
     CDS             complement(7434..8021)
                     /locus_tag="PLJ64_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92555.1"
                     /translation="MKSWQPPLLLVVLIVPAALGFVLAGPGLGLSIAGLSLVGLVVVA
                     MLVIPRDPIGRTPDPELARRLLVVTTFPIEDAVSIQRVADEVRIGFDENDAEIRLLTP
                     ATNTFLRRWATDLERARDNAQRDLVVSVASLTLAGVDASASVGDEDLVQAVEDELVTF
                     DATEVFLLTRGGSGSAAAEQLRERLRPRFHHVDLR"
     gene            complement(8079..8462)
                     /locus_tag="PLJ64_03325"
     CDS             complement(8079..8462)
                     /locus_tag="PLJ64_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNC92556.1"
                     /translation="MSLSECRIAAVAAVSDLDRSRAFFEGTLGLSAVDSGAGGDEVVY
                     SCGEGTGLMVYPSPENAGKGTATLGCWAVDDLESEMKILKDRGIEFERYEGFDQDENG
                     ILDMGDGRVAWFTDPDGNIFAVAEG"
     gene            8542..9486
                     /locus_tag="PLJ64_03330"
     CDS             8542..9486
                     /locus_tag="PLJ64_03330"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNC92557.1"
                     /translation="MSDLDKAVRTVVHECMGVRPNEEVLVVCNPATRELGDALRNEAE
                     AIGAQAVLAVMTERSSHAGEPPATVAEAMVAADVLLAPTVQSLSHTAARKRATESGTR
                     TATLPGATVEMLSRVMSDDMEKLRHRGHRVAELLDRGTEARLTCDHGSNLRLGLQGRV
                     AIPDAGELTETGAFGNLPCGEGFIAPATAEGVLVVDGSIAGVGLVDEPVRLTIKDGHL
                     VDATGEAGAKLMELLTVHGPDGTNVAELGIGTNEKAILTGNILEDEKILGTAHIAFGA
                     SAAIGGNVQVPVHLDVVSMRPEVTIDGTPVVLEGRLLV"
     gene            9483..10382
                     /locus_tag="PLJ64_03335"
     CDS             9483..10382
                     /locus_tag="PLJ64_03335"
                     /inference="COORDINATES: protein motif:HMM:NF019454.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92558.1"
                     /translation="MSLLAVPNVSEGRDFARLDRLEAALGQEVTLLDRHTDLDHHRAV
                     FTLAGPAETLADALTGLAGTATVEIDMTAWDGMHPAIGALDVCPVVWLSERRRQMASE
                     VALEVGEKIGAMGVPVFLYGDLASDADRRERAYFRAGGLDSLWQRMTSGALRPDFGPN
                     QPHPTAGATLVTARAPIAAFNLELATDDLAVAREIAAAVREGPDDGISGVRALGLLLS
                     TGRAQVSTNVHDPIETPLAEIVDAVQEEATLRGTYVVEAELIGLIPEAALEEYPEGVP
                     IRDFDPAFHVIERRLKPRDQAGQ"
     gene            10406..10780
                     /locus_tag="PLJ64_03340"
     CDS             10406..10780
                     /locus_tag="PLJ64_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92559.1"
                     /translation="MAQTKKKRSRKKRGTQAGNLDTSRRSRPRSRAEARNQARSGGGK
                     GGRTMQKGDAPPTWRGATIRGLIAAGVFVLLLLLLFGRPLATSLIIGVVMLIFYIPAG
                     YYMDLYLWRRRERQRIRAKSKG"
     gene            10790..11437
                     /locus_tag="PLJ64_03345"
     CDS             10790..11437
                     /locus_tag="PLJ64_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNC92560.1"
                     /translation="MAGLEVIGTTVGPVAENSYLVFKEETGKALLFDPGDEADRLMQP
                     LADRGLELEAILLTHCHFDHIGAVAPIARATGAPVYCPLIEVPILADVMAYVPWPGIG
                     PYESYDADHTVEGGEHLSLAGLEIDVLFTPGHSPGHVTYSIPEAELLLSGDVLFQGSV
                     GRVDLPGGDWDTLLESIRMLITTFPEQTRVLPGHMGVTSLGDELATNPFLTEIPR"
     gene            11445..12680
                     /gene="hisS"
                     /locus_tag="PLJ64_03350"
     CDS             11445..12680
                     /gene="hisS"
                     /locus_tag="PLJ64_03350"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNC92561.1"
                     /translation="MAEKFKAPRGTFDVLPEQAAQRERLLQAAREIFGLAGYRLIITP
                     AFEDTALFERGVGKSTDIVRKEMFTFEDKGERSLTLRPEGTASVCRAYIEHGMHKLPQ
                     PVKLFYTGPFFRHERPQAGRYRQFNQIGAEAIGSDSPLVDVEVISLLSDLIEELGVPG
                     VELRLGSLGSLDARATYLDELKSHLRSRDQELSEDVRARIEINPLRAFDSDHEGTRAV
                     MADAPHLLDFLSEEDAEHFEQVKSYLGTASVPFTIDPTLVRGLDYYTRTIFSFVCDRL
                     GAQSEIGGGGRYDGLIEQLGGPATPAIGWAAGIERILLALDEEVTSAGMDLFVVVPDP
                     ELITKAVALVSELRHHGLAVEMDLAGRSVKGQFKQADRVNAAHTLILEPGGTASLRNM
                     ETGDQRTVDPKAVANLIRS"
     gene            12765..14267
                     /locus_tag="PLJ64_03355"
     CDS             12765..14267
                     /locus_tag="PLJ64_03355"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HNC92562.1"
                     /translation="MKSLRCLFVSLLALSGLALLPGAAGANLITVTTTTDQFAGLPGT
                     GCSLRDAFNGAVYGGSLGDCELSGADDTIKLPTGSYALTIGPSGDGTPNSGDLDFAGS
                     DSVTIEPEGPNDKVVINGGFVDRVFDHLGAGVGDLILRNITVTGGYPGAGAGFDGGAI
                     RTLNGLVRAEGVTFFGNSSGENGGAIAVGNGASFEAVNSTFTENSAPDSGGGIWTANL
                     GASVDLRNVTISANTADSDADGIGNGGGLSGGIGNSTVNMTNSILAENIDSSPMPADQ
                     VPDCVSNANFFPRFVVSTQQFGAGECLVTLPTPDTNQAPVADAGLQSFADNGGPTPTV
                     ALNANSPAVMAGGSAFPDICLATDQRGAVRPAGRCDIGAFQRTTPLPGVAGPVGSIAA
                     FDGTGLYIRLKCLARFKPKCRSSAVAVTSRSGGRAMTTPKAVVTRSGRYKVLRLTVRP
                     GFRTRVSAMTFVDRRQLVIRQKIRSKRIGNRLAPRRPATVISTYKVRVRT"
     gene            14359..15789
                     /locus_tag="PLJ64_03360"
     CDS             14359..15789
                     /locus_tag="PLJ64_03360"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSLREA domain-containing protein"
                     /protein_id="HNC92563.1"
                     /translation="MKGFLAGAFALLALLVAPAAANAVIITVNTTADEYGTGTSCSLR
                     EAITAAQNNVSFGGCPAGFASDTVSIPDGTYKITRAGTGEDGNATGDFDITGSNALDI
                     QPDASNAHVVVDGNGIDRVFQDLSSGLVKFLAIQVTGGKLTGFNDGAGIQNSVGVTSL
                     ENVTVNDNSSEAQGGGVAVYNNVQVINSTIAQNNADGNGGGFYVPGGASLVARSTTVF
                     GNQADADGDGNGYGGGFAETGGLNVSFTNVLNAGNSGTAILPTDDAYDCYSGPNFYPR
                     YTLSGQPMGPLDCLVGFNPGTNMVSSDVKLDPFLRDNGGQTPTLALLPGSPAIGAGGT
                     ASPDECPGFDQNDRPRPAGKCDIGAVQFVAEPGLIITKLLPKRKVIKRKKTRAITVQV
                     RNDGTGPAKQVKICLKLNRAAKKGLKVKGKACKSVGTVAVGARKSAKIKLMAKPKARK
                     KAYSLKATFKGSNAPLATRAFKVRVK"
     gene            complement(15782..16090)
                     /locus_tag="PLJ64_03365"
     CDS             complement(15782..16090)
                     /locus_tag="PLJ64_03365"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNC92564.1"
                     /translation="MVRFTALLIAAPAFDFFAWTATAPRSTFPELTDREREILALVAE
                     GRSNEQIANRLTLSLKTIRNHVSNIFNKLQVPDRAAAIAKAREAGLGVQPLEASDPPT
                     "
     gene            16077..16283
                     /locus_tag="PLJ64_03370"
     CDS             16077..16283
                     /locus_tag="PLJ64_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92565.1"
                     /translation="MKRTIKPGKTAAVKVTVKPAFRELVDAMTYVDARKLSVAHTVKS
                     KKVGKRTAKKPARPSLSLKVRIKN"
     gene            16299..>17054
                     /locus_tag="PLJ64_03375"
     CDS             16299..>17054
                     /locus_tag="PLJ64_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HNC92566.1"
                     /translation="MSDAPELKPNSYRDTWCGQVLGDKVGGQVRLSGWVHRRRDHGGL
                     IFIDLRDRTGLVQLVFNPDEAGGAHELGHTLRSEYVISVAGEVVTRSDETVNPELPTG
                     EFEVRVKEVQVLAEAETPPFEIEGFHGEVGEEARLKHRYLDLRRDQMQQAIKLRHRLT
                     AAMRSFLDAEGFLDIETPILTLSTPEGARDFLVPSRLQRGSFYALPQSPQLFKQLLMM
                     SGFERYFQIARCFRDEDLRADRQPDFTQLDIEMS"
BASE COUNT         3132 a         5443 c         5660 g         2819 t
ORIGIN      
        1 ggtgtgccgg agtcgccacg cttcaggttg gcgagttggc gctcgcggcg ctcgggatct
       61 ttactgagcg gcatgagcgc ccctttcgtc ggttgccact ctgttgccac ccttgccgca
      121 gacggcatga ctgctgactt tcatggctga tcgtcggatt cggtattcgg ttgtcatggc
      181 cgccgcgccc ctccggcttc gacgtactgc cgaagctcgc cccggtcgaa gagagtcctg
      241 gagccgtctt tgtgaaaagg gatgcgtcgg gcgctgacca gtgcgtagat cctcgacacc
      301 gggcagctca gaaactcggc agcgcccttc acgtcgaggt agccgtcagc agccggttcg
      361 gtcatacggt gagcgagcag ctcggcagct ctcgccgcga ccagctcgac cagctcggcc
      421 gggaccgaca gcgacagcga accgttcggg ccattgcgtt cggggtcggc accgggtcgg
      481 tgcgcgaccg cagcgccagc catgggccgg ttgtttttca ttcctgccct cggcctgtcc
      541 catgtcccac ccctgagaag ggggggtggg acgggacggt ttcagtagtc ccatgggacg
      601 gtttgggacg acttgggaca gggttggcgg tctgggtgag ccggtattcc ttccggtatt
      661 tggtcgcctg ccgctcttcg atcaggtttc gctctacgag tgtgttgagg ctccggtaga
      721 agctcgattt cggaatatcg gaaacctctt tgatcttggt tgccgaaacc cattcggtcc
      781 caaagctctc tgagactgtt tcgaggatcc gctgttcgtg gtccgatagc cgtcccacgt
      841 cgtcgccgag acggattacg cagctttcgt tctggggttc gaggtggaat ctccaggggt
      901 cgaactcggc agcgtctttt tgtttctcgc aggtgagctt caggttcgcg ccgtcgggtt
      961 tgaggcagag catggtgtcg gcggctccgc ggagtgcgga ggagccgcgt tcgagttctc
     1021 cgctcttgcc ggtgtggtga acgattagga gggcggagcc gtggcgcttg gcgagcttct
     1081 cggcgttctg tatgaagagg ccaatgtctc gggctgagtt ttcgtcgccg ccgaccagac
     1141 accgggcgag ggtgtctatg acgatcagga ccgggggctt gtcgagttcg gcgagtgcga
     1201 cttcgagggc tgtcgtttcg gaccggaaga agttgggggc ttcgatccag aatcggaagt
     1261 ccggcacttc ggcaacccct cgggagttgc accatgcctg cgctcggctg tagtagccgg
     1321 aagcgccttc ggccgcgatg tagacgactg gaccctgctg gacggcctga ccgtagaagg
     1381 gttggccggt agctatgcag aggctccagt cgagcgcggc gaagctcttt ccgccgccgc
     1441 tcggcccgta gaggacggtc attcctcgcg cccggatgaa gtcctcaacc aggtagctcg
     1501 gctcgggaag atcggcgagc tggtcgaggg tgagcagccg tggaggatcc gagtcctgga
     1561 cttcggccag acaagcggct tcctgctcgg gggtcagcgg gatcacgccg ccccaagcat
     1621 ttgacgggcg atccatttcc gcagttcgat gaaatcacgg ccccgcgtgt tgagtccggt
     1681 cttggccgcc gcgatggtga agcaagttcc tccaatgccg cagacgtggc agtaccagac
     1741 cgtgtctttg taggtggcgg atgggtggtg gtcttcatgg tcaggcagcg gacagcggca
     1801 cttcccgcgg gtcggttcgc tttcgggggc gagaacggtc aggtacgtct cggccgggat
     1861 cgaggcgagc gcttcggtgc ctcttagcca ggagtcgctc ggcaggttgc tcgcggtccg
     1921 gatcggacga cgcttcggtt tcggtcgccg gtcaatgaac gggccgtcgc catccgcata
     1981 gcggcggtcc tcgacctgaa tggcgaggcg gtcccacatt gactgctgga cgctttcggg
     2041 aagagcatga aagagcagca gccggtcgtg actcgacaga ccgggaagag gacgctggaa
     2101 ctgctggaag gtcggttgcg tcaaaccggc ccctcgacgt ttcggcgatc tcggatgaac
     2161 cggcgcacgt cttcgagttc gtaccgggtc gagcggttcc cgaggacgat tctggtcagg
     2221 tgtcccttgc tcgccagggc gctgacattg cgggtagttg tgccgagtag ctcggcgact
     2281 tccttcggct tcaggagtct cgggatcgca gggtcggaag gtgaaggtgg agtcctgtcg
     2341 gtttgggaag attgggccat atggacaacg tgacaccgac gacaacctcc tccatgagtt
     2401 gtcttgaaac tgggggaggc tggttgcgca accggttggt tctggacgtt cgccgggaac
     2461 ggctcggcgg cccggagtcg gcttcggctt ggatcgaagc tgaccatcag aacggtctga
     2521 cctatgcggc tcgtatcgtc ggagtcggta aatacgcggt catagccgaa gcgaaggtct
     2581 tcccaactca gcctgatcgg gtcgaggcta gaaccgactt tctcggaaga tctcacggcg
     2641 agcgatctcg acggggtggc gagatcgttg atgcggatgc ctcgccgtca ccagggggct
     2701 tgactactgg cgacgtgcga aagatgccca ccgacgctct cctctcaatg gccagagcac
     2761 gactgttgaa gatcgctgag gtgccgtcac ccgtgcttga ctgcttcccc gagctatcgg
     2821 tcccaagacg ggaaggggaa cggcctcaca gacgagatga tcgagcggtg gcaaacgtcg
     2881 ccgcaacgta tgcggaactc gtggaatctg gacacagaaa gccgctcagg agaacggcgg
     2941 aacttcatgg ctacgcaata aagggagtcg aaaaggtcat cgctgccgct agggagcgag
     3001 acatcctcac tccgaccaca cagggaaaag ccggcggcga actgaccaac ctcggcaggc
     3061 aactcgcgca atcccagtaa ggacgatgcg acttcgcccc atctgataat tggaagcctt
     3121 ggataagatt tgacgaccat gctggggagg aagatcgcag tcacgatttg ctgtctgatc
     3181 gcctatgcgg gcgcgcttag cgtccaaggc gcgaccgcag acgtggcggt tcccgtgaac
     3241 atcagcccag cgaacaacgc cgtgcttatc gctgatgacc ctagtccgtg ggtcacgctg
     3301 cccgacgttg cgctcattac gctcaaatat tcatgcgacg caagcaaggc cagctcttac
     3361 ttcgtggaga taacaggcgg ggactcaact ccggggcttc ttggcactac gctgtcctcg
     3421 tataacgacg gggtgtgtga agccaccaat tcagatgggt acgagccagg aacttatacg
     3481 tggcgcccat ttctgatcac cggcctcggg gacgcgggcc aagcatatgg ctccaagtgg
     3541 acctttactg tggaggaacg gccaagcgac cccgatccag acccgatctt ggacacgcac
     3601 atgaacctct cgcaagcgaa gttctacgcg agggcgcaga cgaagaagcg cttctaccga
     3661 gcaccgtttc gagtcagttg ttggcgaaag tcctcctcca gcttcggctg tttcagcttc
     3721 taccgccaga actggcggaa aaggttccgt gcgaagtggg tcttcgcaca cgtagtcgat
     3781 gacaaccagc tctggtacac aacaagcttc aaaggtaaga gccgctactc gggctggtag
     3841 gcatcgccgg actagaacgt tgccactttc gttgccacct tgcggatttt cgggccgaat
     3901 gcgagagtga tttggctcca gctctagtac cgggggaggg actcgaaccc ccgacacgcg
     3961 gattatgatt ccgctgctct aaccagctga gctaccccgg caggggaccg gtgagtctag
     4021 atgatcgggg gctggctggg cctgtcgggg atctcgcccg gggcggcggt gatcaggtcg
     4081 tggggcggcg gaagatcgag cggacgagac cggttgcggt gaccagccag gcggtcaggg
     4141 cgatccagat cgtgtacgac gggatctcct tcatgaagga gaaattgtcg gccgtcgcga
     4201 actgccaggt ggcgaccgtg tacatgccca gagggaagac caggctccag tacatcgggc
     4261 tgtaggtgag cggcaccttt ctcacgacat gccgccagat cccgaagatc accagcaggg
     4321 gaatccacca ggtcgcccag gcccagagca ccagggaggc gccctccacg aacggctcga
     4381 gatcggccag atatccgatc tccgactggg cgatgatcat cgccgaaccg gcgttcaccg
     4441 tgatcgcggc ggcacccatc acgacccaga agagcgggtt catctcctcc ggggtcaccc
     4501 tgaagaagaa gatccggttg gcgaacaggg tggcgaagat tccgtaaagg atcatgccga
     4561 ttccccagaa ggcatgggcg atcacgaaga cgagctcggc gccggtcccg atctgggtgc
     4621 tgagcttgcc gccgagggcg gcgatcgatt ccgccccgac tatcgcgatc agccagccac
     4681 cgttcatcac gccgccgccc tgcctggagt cgcggaaggt gaccaccgcg aagctgaggt
     4741 agctcaaggg aacccagacc acgatcgaaa agatccagag gacgatcgcc gggatcagct
     4801 tgccccgcat gaacagctgg atgccgagca cgttcactcc ggcgaccagg gtgaagaagg
     4861 aaaagacgaa ccgggggtcg accaggtctg ccgcgagttc gcgggggaag agcagggccc
     4921 gcgcgatcgt cgccgtggcc agcaccagga aggcccctac ggcaatgacg atcatcaccg
     4981 ccgacagcac cttgaaatcc agcaggtaga aaccgttcga gatgatgccg gtcgccatca
     5041 ccagcgcgaa gtagcccggg tagagggttc ggatcgattc gcgcagtctt gaatcgccgg
     5101 tcatcgactt ctcagagtag atccctgcgg cgccagagtg cgagcccgcc agccgtgagt
     5161 gccgccgaca gggccgtcat caccagaaga ggagtgaggt cgagcggcac cgccgggacc
     5221 gccggcgtgt gcgagaaggg cgagaggtcg acgatccagt cggggatgtc cccgaaagag
     5281 gccagcgtcc agatcaggac gaaggtggcg aggaggcccc aggcgaccgg gctgagccgg
     5341 gaggaaagcc cgtagagcag cacgacgagg ctgccggtca gccacatcgc gggcgtctgc
     5401 gcgaccgccg ctccggtcag gcggccgatc tggctcgaat caccggtcgc tacggcgaag
     5461 ccgatcccga gacccaggcc gaccagggcg atgatgaccg cggtggccgc gaaggcgatc
     5521 aacaggtggt tggcgaccca gcggaccttg ccgagcgggg cggcgagcag gaggcctacc
     5581 cggccgcgac tctcttccga atgcggtcgc agcacggcac tgaccgtgaa tcccgaagcc
     5641 agcagggcga tcacggtgag gatcgaggcg aaataggaat cgaggaggtt cccgctcgcc
     5701 gatgcgaatg cttcggtgaa ggcggagctc gttttgagca gctgctcgat gttgtcgccg
     5761 atcacgccga ccgcgaatcc gtaggcgaac agcatcaccg cccagccgat gatcgatcct
     5821 cgctgaatgc ggaaggcgaa gccaagcggg tggaggaggg atttgtgggc gcgggccggt
     5881 cccggacggg gctggatcaa tccgccaccg atgtcccggc ggaccagcag ccagccggac
     5941 agggcgaggg tcaccgcgaa ggcggcgagg ggcagcagca gcggccacca gcggtcctcc
     6001 cagaacggcc tggtctgttc ggcccagccg agcggcgaaa accaggtcag cgtgccgccg
     6061 ccggtgtcgc cgatcgcccg cagaagccag gccgccccga tcacgatccc gaccagtccc
     6121 cgcgccgccc ttgccttgct cgtgacctgg cagaccacgg cggtggtccc ggcgatcacg
     6181 gccccgaagc cgaagaagga aagcgacagg agggtcgccg gcccgaccgg gagaccctcc
     6241 gccgcaaaga ccgcgacata gccggccgag gccagcgcca gggcggcaag cgtcacggtc
     6301 agggccgcgg cgagcggggc aagacgcccc gaaccgtagg ccagcaggag ctcggtccgg
     6361 ccgtcctcct cctcggccct ggtgtggcga accacgctga acatcgcgaa gacgccactg
     6421 accaccgaga tcgtcggaag gctgtcccag gtgatcgccc cgcccagcgt gcgaatgccg
     6481 ccggtcggac cggaaatcgc gaccatcgcc ggatttccgg caacgctcct gtaaagcgcg
     6541 tcgagctggg ccgtcgtccc gtaaaggttc ttgtagctga tcacctccat cgcgatccag
     6601 ccgatcagca cggcgaccca gatcggcagg atcagacggt cccgcttcag cgcgaagcga
     6661 atcgcatcag cagttcccgc gaacccgctc atgggttcgc atagatgctg aggaagagat
     6721 cttccaggct gagcgagtac ggatcccggc cggcgctggc ggcgatttcc cgcaggtgcg
     6781 cgagcgttcc ggtctcgacc gtgcggccat ccttgatgat cgtgaaagtg tcggccaggt
     6841 gttccagctg accgaggatg tggctggaaa ggaggatcgt gtggccctgg ccggtgatct
     6901 ccttcacgca ttcctggaag accgcctcct tgagcggatc gaggccggag gtcggctcgt
     6961 cgaagatgaa gagctcggca tcggtggcga gcgccgcgat cagggccact ttctgccggt
     7021 tccccttcga ataggtggag gccttcttgg tcagatccag ttcgaagcgt tcgaccagtt
     7081 ccttgcgcct cccggggtcg agcccgccac gcagcgagcc cagcatgtcg atcgtttcgc
     7141 cacctgacag gttcggccaa aggttcacgt caccgggcac gtatgcgagc cgccggtgca
     7201 gggaaacggc gttcttccag gggtcctcgc cgaagagcct tgcgcttccg ccggaggcgt
     7261 tgatcaggcc gagcaggatc cggatcgtgg tggacttgcc ggagccgttg ggtccgagga
     7321 atccgtggac ctgacccggt tcaaccttga ggtcaaggcc gtccagcgcc ctcgtcgaac
     7381 cgaagttctt gatcaggccc tcgacctcaa tgaccgcgct catggcccgg aaactaccgc
     7441 aggtcgacgt gatggaagcg gggtcgcagc cgctcgcgga gctgctcggc cgcagctgac
     7501 ccggaaccgc cccgggtcag cagaaagact tcggtcgcgt cgaaggtgac cagttcatct
     7561 tccacggcct gaacgagatc ctcgtcaccg accgaggcgg aggcgtcgac gccggcgagc
     7621 gtgagcgagg cgaccgacac gaccaggtcc cgctgggcgt tgtcgcgggc ccgctcgagg
     7681 tcagtggccc agcggcgcag gaaggtgttg gtcgccggag tcagcaggcg gatctcggcg
     7741 tcgttctcgt caaatccgat ccggacttca tcggccaccc gctggatcga aaccgcgtct
     7801 tcgatcggga aggtggtcac taccagcagc cgccgggcga gttcggggtc gggagtccgg
     7861 ccgatcggat cccgggggat gacgagcatc gccacgacga ccaggccaac cagggagagg
     7921 ccggcgatgg acaggccgag gccgggcccc gccagcacga agcccagcgc ggcgggcacg
     7981 atcaggacga cgaggagaag tggcggctgc caggacttca tggccggaaa gtacccgtcc
     8041 ggcccgaaac catccgggcc gggcgcggcg cagcccgatc agccttcggc gacagcgaag
     8101 atgttgccgt ccggatcggt gaaccaggcg acccttccgt cgcccatgtc gaggattccg
     8161 ttctcgtcct gatcgaagcc ctcgtagcgt tcgaactcga tgccgcgatc cttcaggatc
     8221 ttcatctcgg attcgagatc gtcaaccgcc cagcaaccga gggttgccgt gcctttgccg
     8281 gcattctcgg gtgagggata gaccatcagg ccggttccct cgccgcagct gtagacgacc
     8341 tcgtcaccgc cggcgcccga atccacggcc gacaggccga gcgtgccttc gaagaaggcc
     8401 cgggaccggt caagatccga cacagcggct accgcggcga tcctgcattc actcaggctc
     8461 atcgctacct cctgggactg gtgatttgcc tttgtaccca cactagcctc gccgaggctc
     8521 cctgaccact agtttccccc tatgagcgat cttgacaagg cagtcagaac cgtcgtccac
     8581 gaatgcatgg gggtgcggcc aaacgaggaa gtcctggtcg tctgcaaccc ggccacccgt
     8641 gaactcgggg atgccctgcg aaacgaggcg gaagcgatcg gcgcccaggc cgtcctggcg
     8701 gtcatgaccg agcgcagttc acatgcgggc gagccaccgg ccacggtcgc cgaggcgatg
     8761 gttgcggccg acgtgctgct cgcgccgacc gtccagtcgc tctcccacac tgccgcgagg
     8821 aaacgggcga ccgaatccgg gacgcggacg gcgaccctgc ccggggcgac ggtggagatg
     8881 ctttcccggg tgatgagcga cgacatggaa aagctccgcc accgcggcca ccgcgtggcc
     8941 gaactgctcg accgcggcac cgaggcccga ctgacctgcg atcacggcag caacctgagg
     9001 ctgggacttc aggggcgggt cgcgatcccc gacgccggtg agctgaccga aaccggcgcc
     9061 ttcgggaacc tgccttgcgg cgagggattc atcgcaccgg ccactgccga aggcgtgctc
     9121 gtggtcgacg gttcaatcgc cggggtcggg ctcgtggacg agccggtccg gttgacgatc
     9181 aaggacggcc atctggtcga cgcgaccggc gaggcgggtg cgaagctgat ggaactactg
     9241 accgtccatg gtccggacgg caccaacgtc gccgaactcg ggatcgggac caacgagaag
     9301 gcgatcctga ccggcaacat cctcgaggac gagaagatcc tcggcaccgc ccatatcgcc
     9361 ttcggggctt ccgccgcgat cggaggaaac gtccaggttc cggtccacct tgacgtggtt
     9421 tcgatgcgac cggaagtgac gatcgacggg acgccggtgg tgctcgaagg caggctgctg
     9481 gtgtgagcct gctggccgtc cccaacgtct ccgagggtcg cgacttcgcc cggctcgacc
     9541 ggctggaggc ggcgctcggc caggaggtca cgctgctgga ccgccacacc gaccttgacc
     9601 atcaccgggc ggtcttcacc ctggccggac cggccgaaac gctggccgat gcgctgaccg
     9661 gactggccgg cacggcgacg gtggaaatcg acatgaccgc ctgggatgga atgcacccgg
     9721 cgatcggcgc gctggatgtc tgcccggtgg tctggctgag tgaaagacgc cgccagatgg
     9781 ccagcgaggt ggcgctcgaa gtgggggaga agatcggggc gatgggcgtt ccggtctttc
     9841 tttacggcga cctcgcgtcc gacgccgacc ggcgtgagcg ggcctatttt cgggccggcg
     9901 ggctcgattc cctctggcag agaatgactt ccggggcgct caggccggac tttggcccga
     9961 accagccgca ccccacggcc ggggcgaccc tggtcacggc ccgggcgccg atcgccgcct
    10021 tcaacctcga actggccacc gacgacctcg cggtcgcacg cgaaatcgcg gctgcggtca
    10081 gggagggacc ggatgacggc atctcggggg tccgcgccct cggcctgctg ctttccacgg
    10141 ggcgggccca ggtatcgacc aacgttcacg acccgatcga aacacccctg gccgagatcg
    10201 tcgatgcggt tcaggaagag gcgacgctga ggggaaccta cgtggtcgag gcggagttga
    10261 tcggcctgat tcccgaggcg gcgctggagg agtatccgga aggggtgccg atccgggatt
    10321 tcgatccggc cttccacgtc atcgaacggc gcctgaagcc tcgcgaccaa gccggccagt
    10381 agccgttctc cggtaagttt cgcccgtggc acagaccaag aagaagcgca gccggaagaa
    10441 gcgcggcacc caggccggca acctcgacac ctcacgccgt tcgcggcccc ggtccagagc
    10501 cgaagccagg aaccaggcgc gttccggcgg cggcaagggc ggccggacca tgcagaaggg
    10561 tgacgctccg ccgacctggc ggggagcgac catccgcggg ctgatcgctg cgggcgtctt
    10621 tgtcctgctc ctgctgctgc ttttcggccg gccgctcgcc acttcgctga tcatcggcgt
    10681 cgtgatgctg atcttctaca tcccggccgg ctactacatg gacctgtatc tctggcgccg
    10741 gcgcgaacgc cagcggatcc gcgccaagag caagggttag gcacctggca tggcggggct
    10801 cgaggtcatc ggaaccacgg tcgggccggt cgcggaaaac tcgtacctgg ttttcaagga
    10861 ggaaaccggc aaggcgcttt tgttcgaccc cggcgacgag gccgaccggc tgatgcagcc
    10921 gctggccgat cgcgggctgg agctggaagc gatcctgctt acccactgtc acttcgatca
    10981 catcggggcg gtggccccga tcgcgagggc aaccggggcg ccggtctact gcccgttgat
    11041 cgaggtgccg atcctggccg acgtgatggc ctacgtgccg tggccgggaa tcggtcccta
    11101 cgaaagctac gacgccgacc acacggtcga aggcggcgag catctctcgc tggccggact
    11161 tgaaatcgac gtcctcttca cacccggtca cagtccgggc cacgtcacct actcgatccc
    11221 ggaagcggag ttgctgcttt ccggcgacgt gctctttcag ggctcggtcg gccgcgtcga
    11281 cctgcccggg ggtgactggg acacgctgct cgaatcgatc cgcatgctga tcaccacctt
    11341 cccggaacag acccgggtgc tgcccgggca catgggtgtg accagtctcg gggacgagct
    11401 ggcgaccaat cccttcctga ccgagatccc ccggtgagac cctgatggcc gagaagttca
    11461 aggcgccgcg cggcaccttc gatgtgctgc ccgagcaggc cgcgcaacgt gagcggctgc
    11521 ttcaggcggc gcgcgagatc ttcgggctgg ccggctaccg gctgatcatc accccggctt
    11581 tcgaggacac ggccctgttc gagcgcgggg tcggcaagtc gaccgacatc gtccgcaagg
    11641 agatgttcac cttcgaggac aagggggaga ggtcgctgac cctgcggccg gaggggaccg
    11701 cctcggtctg ccgcgcctac atcgagcacg gcatgcacaa gctgccgcag ccggtcaagc
    11761 tcttctacac cgggccgttc ttccggcacg aacggcccca ggccggccgc tatcgccagt
    11821 tcaaccagat cggggcggag gcgatcggtt cggactcacc gctggttgac gtcgaggtga
    11881 tcagcctgct gagcgacctg atcgaagaac tcggggtacc cggggtcgag ctcaggctcg
    11941 gcagcctcgg atcacttgac gcgcgggcca cctatctcga tgaactgaag tcccaccttc
    12001 gctcacggga ccaggaactt tcggaagatg tgcgggcgcg gatcgagatc aaccccctcc
    12061 gggccttcga ctccgaccac gaagggacca gggcggtgat ggccgacgcg ccgcatctgc
    12121 tcgacttcct cagcgaggaa gacgccgaac atttcgagca ggtcaagtcc tacctgggga
    12181 ccgcttcggt tcccttcacg atcgacccga ccctggtgcg ggggctcgat tactacacgc
    12241 gaacgatctt ttccttcgtc tgcgaccggc tcggagccca gtccgagatc ggcggcggcg
    12301 gccgttacga cggcctgatc gagcagctcg gcggaccggc caccccggcg atcggctggg
    12361 cggccggaat cgaacggatc ctgctcgcgc tcgacgagga ggtcacctcg gccgggatgg
    12421 atctcttcgt cgtggttccc gatcccgagt tgatcaccaa ggcggtcgcc ctggtttccg
    12481 agctgcgtca ccacggcctg gccgtcgaga tggacctggc cggccgcagc gtcaagggcc
    12541 agttcaaaca ggccgaccgg gtgaacgccg cccacacact gatcctcgaa ccgggcggta
    12601 ccgcctcgct caggaacatg gaaaccggcg atcagcggac ggtcgacccg aaagccgtcg
    12661 ccaacctgat ccgttcctga cccgaacgcg accgctgcgc aactgccgcc gccttgccgg
    12721 tttagggtgg caacgctgtg tcaactctga atcgggggaa gttcatgaaa tcgctgcgct
    12781 gccttttcgt ctcgctgctc gccctgtccg gtctggccct gttgccaggc gccgcgggcg
    12841 ccaacctgat cacggtgacg acgaccacgg atcagttcgc gggactaccc ggtaccggtt
    12901 gctcgctgcg cgatgccttc aacggggcgg tctacggcgg ctcgctcggt gactgcgaat
    12961 tgagcggcgc cgacgacacg atcaaactgc cgaccggcag ctacgccctg acgatcgggc
    13021 cctccggcga cggaacaccg aactcgggcg acctggattt cgccggcagc gactcggtca
    13081 cgatcgagcc cgaagggcct aatgacaagg tggtgatcaa cggcggtttc gtcgaccggg
    13141 tcttcgatca tctcggggcg ggagtcggtg acctgatcct cagaaacatc acggtcaccg
    13201 gcggctaccc gggggccggc gccgggttcg acggcggcgc gatacgcacc ctgaacggtc
    13261 tggtccgggc cgaaggagtg actttcttcg gcaacagctc cggtgagaac gggggcgcga
    13321 tcgcggtcgg aaacggggcc agtttcgaag ccgtcaactc gaccttcacc gaaaactcgg
    13381 cgccggacag cggcggcggg atctggaccg ccaatctcgg cgcctccgtc gaccttcgca
    13441 acgtgacgat ctccgccaac acggccgaca gtgatgccga cgggattggg aacgggggcg
    13501 gccttagcgg cggcatcggc aactcgacgg tgaacatgac caactcgatc ctggccgaga
    13561 acatcgattc ctcgccaatg ccggccgacc aggttccgga ctgcgtttcg aacgcgaact
    13621 tctttccccg ctttgtggtc agcacccagc agttcggggc cggggagtgc ctcgtcaccc
    13681 tgccgacccc ggacaccaac caggctccgg tcgccgacgc cggacttcag tcattcgccg
    13741 acaacggagg gccgaccccg accgtcgccc tcaacgcgaa cagtcccgcg gtcatggcgg
    13801 gaggcagcgc cttccccgat atctgcctgg cgaccgacca gcgcggagcg gtgcgacccg
    13861 cgggccgctg cgacatcggc gcctttcagc gcacgacgcc gctgcccggt gtggccggcc
    13921 cggtcggatc gatcgccgcg ttcgacggca ccggccttta catcaggctg aaatgcctgg
    13981 cccggttcaa gccgaagtgc cgatcgtccg cggtcgccgt caccagccga agcggcgggc
    14041 gcgcgatgac cacgccgaaa gccgtggtaa ccaggtccgg ccgctacaag gtgctgcgcc
    14101 tcaccgtcag gccgggattc cggacccggg tctcggcaat gaccttcgtg gaccgccggc
    14161 agctggtgat ccgccagaag atccgctcga agcggatcgg caaccgcctg gcaccacgcc
    14221 gcccggccac ggtcatcagc acctacaagg tccgggtcag aacctgagcc ggccggacgg
    14281 cttgacctgc ccaggcgacc cgcatacgat caatccaaag ggccggaacc ggggaggccc
    14341 gaacgactgg aggaaacggt gaagggattt ctggcagggg ctttcgccct tctcgcactg
    14401 ctggtggcgc cggctgccgc gaacgcggtg atcatcacgg tcaacacgac cgccgacgag
    14461 tacggaacgg gcaccagctg ctcgctgcgg gaggcaatca ccgccgcgca gaacaacgtt
    14521 tccttcggtg gctgcccggc tggcttcgcc tccgacacgg tcagcatccc cgatggcacc
    14581 tacaagatca cccgggcggg aacgggcgag gatggcaacg cgaccggaga cttcgacatc
    14641 accggaagca atgccctcga cattcaacct gacgcatcga atgcgcacgt ggtggttgac
    14701 ggcaacggga tcgaccgcgt cttccaggac ctctccagcg gcctggtcaa gttcctggcg
    14761 atccaggtca caggtggcaa gctgaccggt ttcaacgacg gtgcaggcat ccagaactcg
    14821 gtgggagtga cctcgctcga gaacgtgacc gtgaacgaca actcatctga agcccagggc
    14881 ggcggcgtcg ccgtctacaa caacgttcag gtcatcaaca gcacgatcgc ccagaacaat
    14941 gccgacggca acggcggcgg cttctacgtt ccgggcggcg cctcgctggt ggcccggagc
    15001 accacggtct tcggcaacca ggccgacgct gacggtgacg gcaacggcta cggcggaggc
    15061 ttcgccgaaa ccggcgggct caacgtcagc ttcaccaatg ttctgaatgc gggcaactcc
    15121 ggcactgcca tcctcccgac cgacgacgcc tacgactgct acagcggccc gaacttctac
    15181 ccgcgctaca cgctttccgg gcagccgatg ggcccgcttg actgcctggt tggattcaat
    15241 ccgggcacca acatggtcag ctcagacgtc aagctggacc ccttccttcg ggacaacggc
    15301 ggtcagaccc cgaccctcgc cctgctgccg ggatcgcccg cgatcggtgc cggaggtacc
    15361 gccagtcccg atgaatgccc cggctttgat cagaacgacc ggccccgtcc ggccggcaag
    15421 tgcgacatcg gcgcggtcca gttcgtggcc gagcccggtc tgatcatcac gaagctgctg
    15481 ccgaagagga aggtgatcaa acggaagaag accagggcga tcacggttca ggtccgcaat
    15541 gacggaaccg gaccggccaa gcaggtgaag atctgcctga agctgaaccg ggccgcgaag
    15601 aaggggctga aggtcaaggg caaggcttgc aagtcggtcg gcaccgttgc ggttggcgcg
    15661 aggaagtcgg cgaagatcaa gcttatggcc aagccgaagg cgagaaagaa ggcttactcg
    15721 ctcaaggcca ccttcaaggg ctctaacgcc ccgctcgcca cgcgggcatt caaggtgcgg
    15781 gtcaagtagg agggtcagaa gcttcgagcg gctgaacccc gagccccgcc tcgcgggctt
    15841 tcgcgatcgc ggcggcccgg tcgggcacct gcagcttgtt gaagatgttg ctgacgtgat
    15901 tccggatcgt cttcaggctg agggtgagcc gattcgcgat ttgctcgttt gaccgtcctt
    15961 cggccacgag cgcaaggatc tcccgctcgc gatcggtcag ctcgggaaaa gtcgatcgcg
    16021 gggccgtcgc ggtccaggcg aagaagtcga aggccggcgc ggcaatcagc aatgcggtga
    16081 agcggaccat caaaccaggc aagacggccg cggtgaaggt gacggtcaag cccgcgttcc
    16141 gggagctggt cgacgcgatg acctacgtgg acgcccgcaa actttcggtc gcccacaccg
    16201 tcaagtcgaa gaaggtgggc aaacgcacgg caaagaagcc ggccagaccg agcctctcac
    16261 tgaaggtacg gatcaagaac tgaaagactg gcgcttcgat gagcgacgcg ccggagctga
    16321 aaccgaattc gtatcgagat acctggtgtg gccaggtcct gggggacaag gtcggaggcc
    16381 aggtgcgcct ttccggctgg gtccaccgcc gtcgtgacca cggtggcctg atcttcatcg
    16441 acctgcgcga ccggaccggc ctggtccagc tcgtcttcaa cccggatgag gccgggggag
    16501 cgcacgagct cggtcacacc ctgcgttccg aatacgtgat cagcgtcgcc ggtgaagtgg
    16561 tgacccgctc ggatgagacg gtcaaccctg aactgccgac cggggagttc gaggtgcggg
    16621 tcaaagaggt tcaagtgctg gccgaggccg agaccccgcc cttcgagatc gaaggctttc
    16681 acggcgaggt cggtgaagag gcccggctga aacatcgcta cctggacttg cgccgcgacc
    16741 agatgcagca ggcgatcaag ctgcgccacc ggctcaccgc cgcgatgcgc tccttcctcg
    16801 acgccgaggg attcctcgac atcgaaaccc cgatcctcac cctctccact cccgaaggtg
    16861 cccgcgactt cctcgtgcca tcgcgcctcc agcgcggctc gttttacgcc ctgcctcagt
    16921 caccgcagct cttcaagcag cttctgatga tgtccggctt cgagcggtac ttccagatcg
    16981 cccgctgttt ccgcgacgaa gacctgcgcg ccgaccgcca gcccgacttc acccagctcg
    17041 acatcgagat gagt
//
LOCUS       DAOQKN010000029        16869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_1544_length_16869_cov_6.012906, whole genome shotgun sequence.
ACCESSION   DAOQKN010000029 DAOQKN010000000
VERSION     DAOQKN010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 16869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16869
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1544_length_16869_cov_6.012906"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="PLJ64_03380"
     CDS             <1..365
                     /locus_tag="PLJ64_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92567.1"
                     /translation="VDWELCTIGEPLADLGLLMVYWREAGDEQIPLITPATVEPGFPT
                     RAELADLYAEINGRDISKLDYFVALGFWKLAIIAEGVLARFAAGQYGDDIGDVVERSQ
                     ATIDLLVGQADEAINRIA"
     gene            complement(468..1703)
                     /locus_tag="PLJ64_03385"
     CDS             complement(468..1703)
                     /locus_tag="PLJ64_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNC92568.1"
                     /translation="MDFEAGSEVEGLLERVRSFMDEHIYPREAELHQAIDDEVKAGVA
                     YPVELEQIREKAKSEGLWNLFLPDEHYGAGLNNWQYAFLCEEMGRSPLVAPMAFNSAA
                     PDTGNMEILLEHGNDEQKAKYLAPLTDGEIRSCFSMTEPDTAGSDPTGLRAKAELVDG
                     EWVINGHKWFTSGAVGAEFAIAMVNTEESDNPYANASMILVPLDAPGFNLVRPVPVMG
                     HSKGPGHCEILYEDCRVPEENLLGARGAGFLIAQDRLGPGRIHHCMRAIGTAERALEM
                     MCLRAADREAFGGPLADKQFVQDFIARSRMEIDQARLLSLNAAWMMDTVGKHAARQEI
                     SMIKVVAANMVMDVLDRALQVHGALGMSDDTPLAAMWRYSRMLKLADGPDEVHKMVIA
                     RREVGRFLKKRAASNGQAA"
     gene            complement(1740..2468)
                     /locus_tag="PLJ64_03390"
     CDS             complement(1740..2468)
                     /locus_tag="PLJ64_03390"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNC92569.1"
                     /translation="MRRLIPDPGPTDLESELEAYRPFDEAKDDRPFVAVNMVTTLDGR
                     AAVSGNTKALGSDADTEHLLGLRTRFDAVLIGGGTMRAERYGRIISRPEYRRRREAAG
                     LDGDALAVIISGDLDLPFDAPLFTNGEGRVLIFSRKKSLPETETPVELVTIDGLIDSA
                     EVLAHLRRNEGVRAVLCEGGPRVFGQLVAGDLVDELFLTMTPVATGGDATHILEANLP
                     REARFELTGLLEADGDLFTRYRRA"
     gene            complement(2517..3509)
                     /locus_tag="PLJ64_03395"
     CDS             complement(2517..3509)
                     /locus_tag="PLJ64_03395"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNC92570.1"
                     /translation="MSETADTKVTAPEATYDPADEAMCDRARERFTADRNRRLPRRKS
                     KTATLPNMIIIGGLKCGTTSIHHYLGVHPDIHMSKPKELNFFAAEQNWDLGFDWYENR
                     FDSTVRINGESSPHYTNRPRFNDVAERIHKHIPDVKLIYMVRDPIKRILSHWVHATGA
                     GYETREFVETLSLPDTAYIQRSQYWMQLQPYLEYFDRDQIKVISQEDLGKKRDETMKD
                     AFAYLGVDESFTSEQWDRQWEKSSAKHDDKYQFMEKLIKLPGFRAFDRNFDRLPESMR
                     WMVEKVVHDPDKPPAPKPEIPDDLMETLKDRFRDDVAGLKQWSGRDFDGWHDYS"
     gene            3531..4412
                     /locus_tag="PLJ64_03400"
     CDS             3531..4412
                     /locus_tag="PLJ64_03400"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 family protein"
                     /protein_id="HNC92571.1"
                     /translation="MTGTIPSATADDRSDQALSWRGPGPGRPDLPIPPARQPLLHRGL
                     MRKKWRYVGFYGDEVMLCAAIARIGPFSHSFWSVWDRIEGKVWEHTRLRPGGQEVVLD
                     GPVVEIRTPNVRAAIRFGELEPIEVIAPSGKGWAWTRKRAGVPVSGRIITDGKIREVR
                     GFGVDDESAGYHQRNVKWKWSAGIGEATDGRALGWNLVEGINDSPQQSERAIWLEGVP
                     GEPGPVSFDGLDAIRFGEPDEGLVFDFGGAERKRHDNFELIRSDYTHRFGTFTGRLDG
                     IELASAAGVTEEHAVVW"
     gene            complement(4420..5109)
                     /locus_tag="PLJ64_03405"
     CDS             complement(4420..5109)
                     /locus_tag="PLJ64_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92572.1"
                     /translation="MFESARQRSGGAAGSATLEQVGLILLLAAAFTGLIAATVTGVVE
                     PPGHGLGIRIANRIACGPREPGVCRQHPAVSAYGWPLARALRWLAPDPVARIGPEGTP
                     LAPVDFRYCQRPGCAVPAGDGRLTTANRRLTLFTEVKRLPSGVTGGKSAKLEFTYWFY
                     RPSLGWEKVVRQAGPAEIAAASGTRLLLEDSPRLVPLEILPGRNHYDLPAGDEPPWRW
                     KVTPSYEGWSD"
     gene            5251..5520
                     /locus_tag="PLJ64_03410"
     CDS             5251..5520
                     /locus_tag="PLJ64_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92573.1"
                     /translation="MEGSPFNMFGDPDELRARMQEMADQMQSSQEVAWADNAIKLAVD
                     MTVASIGRLELTGSSDEQAMQVRDAIRVVFPEAVTLVREARQGLR"
     gene            5565..6257
                     /locus_tag="PLJ64_03415"
     CDS             5565..6257
                     /locus_tag="PLJ64_03415"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC92574.1"
                     /translation="MIHGTGTDSSVFDEAVGSLAGSWRTVTVDRRGWGRSVPPDEYRR
                     TSIAEQAIEVAAVLRELKVEDATLVGLGFGAVVALEIALADPELAARSFLIEPPVFGA
                     LKEATEGMSADVDAIRAAAGEGGEEAAYELFLTGRLHTLGVGAERFADHADRGPAAAR
                     SFLIEIPAVPAWSLDPVRLAGLESEVTVVTLPSTPDLLGRASQAVADRVPAAATIHSS
                     RDGIAAVAALLA"
     gene            complement(6254..6955)
                     /locus_tag="PLJ64_03420"
     CDS             complement(6254..6955)
                     /locus_tag="PLJ64_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92575.1"
                     /translation="MTIDLPHVPLEAGSGPENPPCPACGEPLFPWVGMPVGSGIAHRC
                     EACGLGVLSHGEKFSFPPPVGMEGATDRGKGPDSFDPGTSVDALRELDSERDEDGSYL
                     FENRASLVCWVTGGAWVGLGTDRRYRFTPAAVTDLIASRDQVVTKVRWRPLRGIAVTW
                     QSGLNMFTFGQNTVLGSLGKVFGVPADRPWKRGLDWFISVAVAIPAIIVAVPLELIGI
                     LFRRGASARAEVQVL"
     gene            6978..7151
                     /locus_tag="PLJ64_03425"
     CDS             6978..7151
                     /locus_tag="PLJ64_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92576.1"
                     /translation="MSSSDSDPGRIESLIVAVSPLLDLVLAAGERISRIAEPVDYEYY
                     PVRDKDEEERSGE"
     gene            7188..8117
                     /locus_tag="PLJ64_03430"
     CDS             7188..8117
                     /locus_tag="PLJ64_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92577.1"
                     /translation="MNSQSEILEREKRWSLIAGIASVGAVALFFIGQIVGRSGVGSPE
                     GTADFLAALSGHHSTAMLGAVIQGIATVLLAVPLAYLFFAALGRSDRMRRGLIAVVIA
                     GPLFVGIGSIISTSSLLSAADQWKGDQTPAVVKCIDEAQGDSADEVTEEQKDTCRDDA
                     ATDLRNDQSLTNLAVGLSLSGALGFLIALMYTSLNAMRVGLLTRFWGSLGMAVGVLLI
                     FPVLQYVALAWFIFLGFLIVGWLPGGRPEAWSAGVAVPWPVPGEIRQDQDETVIEGSA
                     EEVDPSAGDLEAGEPEGSDEGPQGELRKRKKRN"
     gene            8146..9759
                     /locus_tag="PLJ64_03435"
     CDS             8146..9759
                     /locus_tag="PLJ64_03435"
                     /inference="COORDINATES: protein motif:HMM:NF019520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HNC92578.1"
                     /translation="MASQSLTVAEFIELPIVKAGLPELVAGGEGLDAEVRWVHPLDVP
                     DVSDLLRGGEMILTTGVSIGEDATAQRRFVRDLEAEGAVGIAVELGFAWSRELPKALV
                     DEADRRHIPMVAFRRGIRFVEVSEVVNGSLLDSGHALARRGEELHRSLDRLVLEGEGA
                     ESVLAEVSRRIGNPVVLEDARGELVAVGSATRPESEVVDTWSGLKWSDRNHAEAEGAL
                     AAQVMVRGRNWGRVIALQTDGEFDRFTPIALERAAVAVGLGLMRGGEEAELRARSRGN
                     LLFELAAGLMEPLAAARRASTMGFPRRHGQLVPIAAVWRAGALEKGVGYEEAWAPILP
                     AVRAALAEPDRPALLGSRGNCMLGVLDRGTLGSEPDDVASRERLSRRFAGVLARHEID
                     PGSVALAIGGAAGSWEELAPALVRARFAAEAGAAGPLRAWHDASRTEIGQLLLSMRGT
                     PELLEFSDERLAPLLDLPGERGRDLLDTLEVYLHSGGRKTEAATELEIERQSLYHRLS
                     RIEEVLDVDLKDGDTVLALHLAIMINRLL"
     gene            complement(9769..10359)
                     /locus_tag="PLJ64_03440"
     CDS             complement(9769..10359)
                     /locus_tag="PLJ64_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92579.1"
                     /translation="MSDLTGQPELSDRLSQIPKNEPFPVVGLVIFFVGMVMVAYGITA
                     LWFGMREVMDVGGYCAEGGPYVVRQHCPDGAEMLMFTGIPIGIIGLFIALLGGAKSAK
                     GAAGLLLLGWPALFISLGYNFIDYAINPPEGMGSTAGWWVCGILFALMGLPALAGIPM
                     LVKAIQPERRNAVLAVFLIAAAAGVVIGIQVANAAD"
     gene            complement(10370..12361)
                     /gene="acs"
                     /locus_tag="PLJ64_03445"
     CDS             complement(10370..12361)
                     /gene="acs"
                     /locus_tag="PLJ64_03445"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HNC92580.1"
                     /translation="MPEKSELDVLLKTEQTFPPPEGFAAASEFNDPGIYEQADADPEG
                     WWASWAEKLDWIEPWDTILDWSNPPHVTWFAGGKLNVSANCLDRHIEAGRADDIAYHW
                     EAEDAGPDDEPVTGVGSHAGGRISVTYGWLHDQTQRLANVLRDRGVGKGDVVGIFMPM
                     VPEAAVAMLACTRIGAIHNVVFGGFSAESLRERMEFSDAKALITADATLRRGNPMPMK
                     AAIDEALDTLPAMEHVIVLDRCGTGAPLKEGRDLDWAEALAAADPVCEPEPMDAEDPL
                     FLLYTSGSTAKPKGIVHTTGGYLTQATATHRMIFDLKDDDVYWCAADIGWVTGHSYIV
                     YAPLANGATSIMFEGAPDFPTKDRWWEIVERYRATILYTAPTVIRSFMKWGEELPAAH
                     DLGSLRLLGTVGEPINPKAWLWYNEHIGGGRCPIVDTWWQTETGAAMISPLPAITTTK
                     PGSATRPLPGFRAAIFDNNGNEIEEGGGTLVLTRPWPAMARTLFKEPERYINAYWDRY
                     GKETYLVGDAARRDEDGYFWIVGRIDDVINVSGHRLSTMEIESSLVSHPHVAEAAVIG
                     VPDDFTGQAIIAFATPNDEAPPGHEFTDELREHVADRIGKLARPKAIYFADDLPKTRS
                     GKIMRRLLRDIADGKELGDVSTLRDPAVVEELQAQVAAS"
     gene            complement(12491..13096)
                     /locus_tag="PLJ64_03450"
     CDS             complement(12491..13096)
                     /locus_tag="PLJ64_03450"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNC92581.1"
                     /translation="MFELSFISPVSRLAKQSSLLAAFLVAACLLVGVNGQLGAKPADA
                     ASLIAPAKTCPNRAASGGKVAMAKARKSMICMVNYARGREGLRPYRTRQQLTWSSRHK
                     ARDILRCGFSHEACGRSFDFWIKRSGYLGEGGWATGENIAWGSGSLGDVRSIFIAWMK
                     SKGHREAILSRTFTDVGTGVVKGTFQSFHGARIWVLHFGDN"
     gene            13096..13500
                     /gene="msrB"
                     /locus_tag="PLJ64_03455"
     CDS             13096..13500
                     /gene="msrB"
                     /locus_tag="PLJ64_03455"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HNC92582.1"
                     /translation="MSSDSTPLSRSDAEWRESLSPEAYEVTRNAATERPFTGPHLDNK
                     QDGSYTCVCCGAELFSSDAKFDSGSGWPSFTEPANLEHVELRQDNSHGMVRTEVVCRN
                     CDAHLGHVFPDGPAPTGQRYCINGCALEFNEK"
     gene            complement(13575..14231)
                     /locus_tag="PLJ64_03460"
     CDS             complement(13575..14231)
                     /locus_tag="PLJ64_03460"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC92583.1"
                     /translation="MTDHRVLICDDHWIVRQAIRQRIDDLEGFTVVEEVGDGAEVKPA
                     VRKLHPDLVLLDIEMPNVNGIESMRRILSRFPEIRILVYTAHDDPSLVRLASREGASG
                     FLVKSADLDQIRDACETVMAGERWFPGYTDKDLQQEDELKRFMELSDREREVLALLAT
                     GMRAQGAAEEIGIRTATVYTHVRNVVNKLGVDTRTQAVAIATRYAFLDRDPEKPPTAR
                     "
     gene            complement(14228..15493)
                     /gene="serS"
                     /locus_tag="PLJ64_03465"
     CDS             complement(14228..15493)
                     /gene="serS"
                     /locus_tag="PLJ64_03465"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNC92584.1"
                     /translation="MLDLKLIRSDPERIKAALARRGAADQVDALLTLDERRRQLLPEI
                     EGARSERKQESEKIGALKKAGEDASAQMEAVRQLKARLEEMEAELEAVDGKLREVAAT
                     LPNLPDPTSPDGYTDEDAELIREVGEIPDFGFEPRDHVELAGDSIELEAAAKVSGARF
                     AYLKGDLVMLEFALVRWALEKLRAEGHEPVVPPILVREEALFGTGFLPGDREQIYELE
                     KDELFLAGTSEVPLAGLHADQILEADQLPLRYAGFSSCFRREAGAAGRDTHGIFRVHQ
                     FDKVEMFSFVKPEDAGAEHERILAIEESILTELGLPYRVVNVAVGDLGGSAAKKYDCE
                     AWIPSQGRYRELTSCSNTTDFQARRIGSRYRPEPGAPPEFVNTLNGTAVAVGRTIIAL
                     LENHQQADGSVRLPESLVSYGAPAVIGGN"
     gene            complement(15509..15955)
                     /locus_tag="PLJ64_03470"
     CDS             complement(15509..15955)
                     /locus_tag="PLJ64_03470"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNC92585.1"
                     /translation="MRAVAITTLAVAAALGLSACGFGEEGITVSKDSPDYEGAQLFAA
                     NCAGCHTLTPAGALGTGNRGTRNQGPNLDQRAVTYEDALHAIQNGGFSGAIMPQNIVV
                     GEEAEQVARFVAEYAGSDAGEAGAEAYSAEEDASSPQDEPIPTDGQ"
     gene            15988..16482
                     /locus_tag="PLJ64_03475"
     CDS             15988..16482
                     /locus_tag="PLJ64_03475"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNC92586.1"
                     /translation="MAAPDRNEFRQTVGLLPTGVTVVSAFQASGPAGATASAVCSLSL
                     EPMMMLVCLDLGSRSLEAVREAGRFGISVLGESQRAAAEMFATKADQEEKWSSVVWRE
                     HLEVPIIEGSLARVVCDLAEVIPGGDHVIVTGEVLEVEAEDEPGEPLIYHGGLFRRLD
                     AQQD"
     gene            16520..>16869
                     /locus_tag="PLJ64_03480"
     CDS             16520..>16869
                     /locus_tag="PLJ64_03480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HNC92587.1"
                     /translation="MSSHPFFWLDVFAESPLAGNGLAVVLDADGVSDEIMLAFARETG
                     LSETTFVQSPSSEAASSGADYRNRIWTIATEVPFAGHPSLGTAVAVALDRGVEEITFT
                     QETGAGLQQVEVSRA"
BASE COUNT         2695 a         5491 c         5727 g         2956 t
ORIGIN      
        1 tggtcgactg ggaactctgc acgatcggcg aaccgctggc cgacctcggt ctgctgatgg
       61 tctactggcg cgaggccggt gacgaacaga tcccgctgat cacacccgcg accgttgaac
      121 cgggtttccc gacgcgggcc gagctggctg atctttacgc ggagatcaac gggcgggaca
      181 tctcgaaact cgactacttc gtggcgctcg ggttctggaa gctggcgatc atcgccgaag
      241 gcgtgctcgc ccgtttcgcc gccggccagt acggcgacga catcggcgac gtggtcgagc
      301 gctcacaggc cacgatcgat cttctggtcg gccaggccga cgaggcgatc aaccggattg
      361 cataagtaag tcgggcgggg tccgacccgc ccgaggtcag gccgcctgac cgttggaggc
      421 ggcccgcttc ttcaggaacc gtccgactac accagggtgt ccggaggtca ggccgcctga
      481 ccgttcgagg cggcccgttt cttcagaaag cggccgacct cccgcctcgc gatcaccatc
      541 ttgtggacct cgtcgggacc gtcggcgagc ttaagcatgc ggctgtagcg ccacatcgcg
      601 gcaagcggag tgtcatccga catgccgagg gcgccatgga cctggagggc ccggtcaagc
      661 acgtccatca ccatgttggc ggcgaccacc ttgatcatcg agatctcctg gcgggcggca
      721 tgcttgccga ccgtgtccat catccaggcg gcgttcagcg aaagcaggcg ggcctggtcg
      781 atctccatgc gggaccgggc gatgaaatcc tggacgaact gcttgtcggc cagcggcccg
      841 ccgaaggcct cgcgatcggc ggcgcgcagg cacatcatct ccagcgcccg ttcggcggtc
      901 ccgatcgccc gcatgcagtg atggatgcgc ccgggcccga gacggtcctg ggcgatcagg
      961 aagccggccc cccgggcacc gagcaggttc tcttccggaa cccggcagtc ttcgtaaagg
     1021 atctcgcagt ggccgggacc cttggagtgg cccatcaccg gtaccggccg gaccaggttg
     1081 aagccgggtg cgtccagtgg cacgaggatc atcgaggcgt tggcgtaggg gttgtcgctc
     1141 tcctccgtgt tgaccatggc gatcgcgaac tcggcgccga ccgccccgga ggtgaaccac
     1201 ttgtgcccgt tgatgaccca ctcgccgtcg acgagttccg ccttggccct gagaccggtc
     1261 gggtccgagc cggcggtgtc cggttcggtc atcgagaagc agctgcggat ctcgccatcg
     1321 gtcagcggcg ccaggtactt cgccttctgt tcgtcgttgc cgtgctcaag caggatctcc
     1381 atgtttcccg tgtccggggc ggcgctgttg aaggccatcg gggcgaccag cgggctgcgg
     1441 cccatctcct cgcagaggaa ggcgtactgc cagttgttga ggccggctcc gtaatgctcg
     1501 tccggcagga agaggttcca gagaccttcc gatttggcct tctcgcggat ctgctcgagt
     1561 tcgacgggat aggcgacccc ggccttcacc tcgtcgtcaa tcgcctggtg aagctccgct
     1621 tcgcgcgggt agatgtgctc gtccatgaag gaacggacgc gctcgaggag cccttcaact
     1681 tcggaaccgg cttcgaaatc catggccggg acgctactac ccgccgatct gttgcagcgt
     1741 caggcgcggc ggtaccgggt gaagaggtcg ccgtcggcct cgagcagccc ggtcaactcg
     1801 aatcgggctt cccttggcag gttcgcttcg agaatgtgcg tggcgtcgcc gccggtggcg
     1861 actggcgtca tggtcagaaa gagctcgtcg acgaggtcgc cggcaaccag ctggccgaat
     1921 acacgcgggc cgccttcgca aagcaccgcg cgaactccct cgttccggcg caggtgagcg
     1981 agcacttcgg ccgagtcgat caacccgtcg atcgtcacca gctcgaccgg tgtctctgtt
     2041 tcgggcaggg acttcttgcg cgagaaaatc aggacccgcc cctcgccgtt cgtgaagagc
     2101 ggagcatcga aaggcagatc gaggtcgccg ctgatgatca ccgccagcgc gtcgccatca
     2161 agtccggcgg cttcgcggcg ccggcggtat tccggacggg agatgatccg gccgtaccgt
     2221 tccgcccgca tcgtgccccc gccgatcaac accgcgtcga agcgcgtcct gaggcccagc
     2281 aggtgctccg tatcggcgtc ggagccgagc gccttggtgt tgccactgac cgcggcgcgg
     2341 ccgtccaggg tggtgaccat gttgaccgcc acgaagggac ggtcatcctt cgcctcgtcg
     2401 aaggggcgat aggcctcgag ttcggattcg aggtcggtcg gcccgggatc agggatcagg
     2461 cggcgcatgg gatgaggcta accggagcgg cgggcgcgct ttccgggcgc gactgctcag
     2521 gagtagtcgt gccagccgtc gaagtcgcgg cccgaccact gcttcaggcc ggcgacgtca
     2581 tcgcggaacc ggtccttgag ggtttccatc aggtcatccg ggatctccgg cttcggggcc
     2641 ggcggcttgt ccgggtcgtg aacgaccttc tcgaccatcc agcgcatcga ctccggcaga
     2701 cggtcgaaat tccggtcaaa ggcacggaat ccgggcagct tgatcagctt ttccatgaac
     2761 tggtacttgt cgtcgtgctt ggccgaagac ttctcccatt ggcggtccca ctgctcggag
     2821 gtgaagctct cgtcaactcc gaggtaggcg aacgcatcct tcatcgtctc gtcccgcttc
     2881 ttgccaaggt cttcctggga gatgaccttg atctggtccc ggtcgaagta ctcgaggtaa
     2941 ggctgcagct gcatccagta ctgcgaacgc tggatgtagg cggtgtccgg gagcgagagg
     3001 gtctcgacga attcccgggt ttcataaccg gctccggtcg cgtggaccca gtgggaaagg
     3061 attcgcttga tcgggtcgcg gaccatgtag atcagcttca cgtcggggat gtgcttgtgg
     3121 atccgctcgg cgacgtcgtt gaagcggggg cggtttgtgt aatgcgggga ggattcgccg
     3181 ttgatcctga cggtcgaatc gaagcggttt tcgtaccagt cgaaaccgag atcccagttc
     3241 tgttcggcgg cgaagaagtt gagttccttc ggcttcgaca tgtgaatgtc ggggtggacg
     3301 ccgaggtagt ggtggatcga agtggttccg cacttcaggc cgccgatgat gatcatgttc
     3361 ggcagggtcg cggtcttgct cttgcggcgc ggcaggcgac ggttgcgatc ggcggtgaag
     3421 cgctcgcggg cgcggtcgca catcgcttcg tcagcggggt cgtaggtggc ctcgggggcg
     3481 gtcacttttg tatcggcggt ttcagacacg gcggagtagt gtagccggga atgacaggca
     3541 cgatcccctc cgccacggct gacgaccggt ctgaccaggc actttcgtgg cgcggacccg
     3601 gtcccgggcg ccccgatttg ccgatccctc ccgcgcgcca gcccctcctt caccgcggcc
     3661 tgatgcgcaa gaagtggcgc tatgtgggct tctacggcga cgaggtcatg ctttgtgccg
     3721 cgatcgcccg gatcggtcct ttcagccaca gcttctggtc ggtctgggac cggatcgagg
     3781 gcaaggtctg ggaacacacc aggctcaggc ccggagggca ggaagtggta ctcgacggtc
     3841 ccgtggtcga gatccggacc cccaacgtga gggccgcgat ccgcttcggt gagctcgaac
     3901 cgatcgaagt gatcgccccg agcgggaagg gatgggcatg gacccgcaag cgggccggag
     3961 tgccggtcag cgggcgaatc atcaccgacg ggaagatccg tgaagtgcgc gggttcggag
     4021 tcgacgacga gtcagctggg taccaccagc gcaacgtcaa atggaagtgg tcggcgggaa
     4081 tcggcgaggc cactgacggc cgggcgctcg gctggaacct ggtcgaagga atcaacgact
     4141 ctccccagca aagtgaacgg gcgatctggc tcgagggcgt gcccggtgag ccgggaccgg
     4201 tcagcttcga cggtctcgat gcgatccggt tcggagaacc ggatgaaggc ctcgttttcg
     4261 acttcggtgg agccgaacga aaacgccacg acaacttcga gctgatccgc tccgactaca
     4321 cccaccgctt cggcacattc accggaaggc tcgacggcat cgaactcgcc tcggccgccg
     4381 gggtgacgga agaacacgcg gtcgtctggt gagccggtct cagtccgacc agccttcgta
     4441 gctcggggtc accttccagc gccagggggg ttcgtcaccg gccgggaggt cgtagtggtt
     4501 tcggccgggc aggatttcca gaggcaccag acgcgggctg tcctcgagca gcagcctggt
     4561 tccggaggcc gccgcgatct cggccggacc ggcctgccgg accaccttct cccagcccag
     4621 cgagggccgg tagaaccagt aggtgaactc cagcttcgcc gatttgccgc cggtcacgcc
     4681 ggacggcaac cgcttcacct cggtgaagag ggtgagcctg cggttggcgg tggtcagcct
     4741 gccgtcgccg gccggcaccg cgcagcccgg tctctggcag tagcggaagt cgaccggggc
     4801 cagcggcgtc ccttcggggc cgatccttgc gacggggtcc ggcgccagcc agcgcaatgc
     4861 ccgggcgagc ggccagccgt aggccgagac cgccggatgc tggcgacaga cccccggctc
     4921 gcgtgggccg caggcaatcc ggttggcgat cctgattccc aatccgtgcc ctggcggttc
     4981 gacgactccg gtcacggttg cggcgatcag gccagtgaag gcggcagcca gaagcaggat
     5041 cagcccgacc tgctcgaggg tcgccgatcc agccgccccg ccgcttcttt gccttgccga
     5101 ttcgaacatg acccgaagct acggacgaat tgctcacatg tggctctaca ggtgcaacga
     5161 ttcagttaca ggcgagcgga aaggaagcgc atcccgaccg tgagggccgt atctgcgcgg
     5221 ccggacactg cgggtcgcta gattggtctc atggaaggtt caccgttcaa catgtttggc
     5281 gaccccgatg aactgcgtgc ccggatgcag gagatggccg accagatgca gtcctcccag
     5341 gaagtcgcct gggccgacaa cgcgatcaag ctcgccgtcg acatgaccgt cgcctcgatc
     5401 ggcagactgg agctcacggg aagctccgac gagcaggcga tgcaggttcg tgacgcgatc
     5461 cgcgtggtct ttcccgaggc agtgaccctg gtccgggaag ctcgccaggg actccgctga
     5521 gcgatctcaa cttcatcgac cggggcgaag ggccgccggt cctgctgatt cacggaaccg
     5581 gcaccgattc ctcggtgttc gacgaggcgg tcggctcgct ggccggttcg tggcgaacgg
     5641 tcacggtcga ccggcgcggg tggggccggt cagttccgcc ggatgaatac cggcggacct
     5701 cgatcgctga acaggcgatc gaagtcgctg cggtcctgcg cgaactgaag gtcgaggatg
     5761 ccaccctggt cgggctcggt ttcggcgcgg tcgtggccct ggagattgcc ctcgccgacc
     5821 ccgaactggc agctcgctcc ttcctgatcg agccgcccgt cttcggcgcc ctgaaggaag
     5881 cgaccgaggg gatgtcagca gacgtcgacg cgatccgggc ggccgccgga gagggcgggg
     5941 aggaagccgc ctacgaactc tttctgaccg gccggcttca caccctcggg gtcggggccg
     6001 aacgcttcgc cgaccacgcc gaccggggtc cggccgcggc ccgttcattc ttgatcgaga
     6061 ttccggcggt tcccgcctgg tccctggatc cggtccggct ggccggactc gaatcggagg
     6121 tgaccgtcgt taccctgccg tcaaccccgg acctgctcgg ccgggcctcg caggctgtcg
     6181 ccgaccgtgt gccggcagcg gcgaccattc attccagccg ggacggcatc gccgccgttg
     6241 cggccttgct cgcctagagg acctggactt cggcccgggc cgaggcgccg cggcgaaaga
     6301 ggatcccgat cagctcgagc gggacggcga cgatgatcgc cgggatcgca accgcgaccg
     6361 agatgaacca gtcgagcccg cgcttccagg gccggtcggc cggaacgccg aagaccttgc
     6421 ccagcgagcc gagcacggtg ttctggccga aggtgaacat gttcagtccc gactgccagg
     6481 tgaccgcgat gccccgcagc ggccgccagc gcaccttggt cacgacctgg tcgcggctcg
     6541 caatcaggtc ggtcaccgca gccggagtga accggtagcg acgatcggtc ccgagtccga
     6601 cccacgcccc tccggtaacc cagcaaacca gcgaggcccg gttctcaaag aggtaggaac
     6661 catcctcgtc ccgttccgaa tccagctcac gaagcgcgtc gaccgaggtt ccgggatcaa
     6721 acgaatcggg ccccttgcca cgatccgtgg ctccctccat tccgaccggc ggcggaaagc
     6781 tgaacttctc accgtggctg aggacaccga gcccgcaggc ctcgcagcgg tgggcgatcc
     6841 cggagccgac cggcatgccg acccagggga agagcggctc gccgcaggcc gggcagggag
     6901 gattttccgg accggatccg gcttcgaggg gaacgtgggg cagatcgatc gtcaaactcg
     6961 ctgtagcgta ttgccccatg agttcatccg actccgaccc cggcaggatc gaatcgctga
     7021 tcgtcgccgt atcgccgctg ctggatctgg tcctcgcggc cggtgagcga atctcgcgga
     7081 tcgccgaacc ggtcgattac gagtactacc cggtccggga taaggacgaa gaggaacgct
     7141 ccggggagtg accggcctgt aggcttgccc ggccttcggg cccacccatg aattcacaga
     7201 gcgagatact cgagcgcgag aagcgctggt ccctgatcgc gggcatcgct tcggtcggag
     7261 ccgtcgccct cttcttcatc gggcagatcg tcggccggtc cggagtcgga tcgcccgagg
     7321 ggacggccga tttcctggcc gccctgtccg gtcatcactc gaccgcgatg ctgggcgcgg
     7381 tcatccaggg catcgccacc gtcctgctcg cggtcccgct cgcctacctg ttcttcgcgg
     7441 cgctgggccg atccgaccgg atgcgccgcg gcttgatcgc ggtcgtgatc gccggccccc
     7501 tcttcgtcgg catcggctcg atcatttcga cctcttcgct gctcagcgcg gctgaccaat
     7561 ggaagggcga ccagactccg gccgtcgtca agtgcatcga cgaggctcag ggagattccg
     7621 ccgacgaggt caccgaagaa cagaaggaca cctgccgcga cgatgcggcg accgacctgc
     7681 gcaatgacca gagcctgacc aacctcgcgg tcgggctttc actctccggc gcgctcggct
     7741 tcctgatcgc actcatgtac acctcgctca acgcgatgcg ggtcggcctc ctgaccaggt
     7801 tctggggctc actcgggatg gcggtcggcg tcctgctgat cttcccggtg cttcagtacg
     7861 tggcccttgc ctggttcatc ttccttggct tcctgatcgt cgggtggttg cccggcggca
     7921 ggccggaggc gtggtcggcc ggcgtcgccg ttccctggcc agtgcccggt gaaatcaggc
     7981 aggaccagga tgagacggtg atcgagggtt ccgccgaaga ggtcgatccc tcggccggag
     8041 atctcgaagc gggcgagccc gaagggtccg atgaagggcc gcagggcgag cttcgcaagc
     8101 gcaagaaacg gaactgaagc cgcttccttc tgagacgatg gcggcatggc ctcgcagtcg
     8161 ctgaccgtcg ccgaattcat cgagctcccg atcgtcaagg ccgggctgcc cgaactcgtg
     8221 gccgggggcg aaggtctgga cgcggaagtg cgatgggtcc atccgctcga tgtcccggat
     8281 gtgtccgatc tgcttcgcgg aggggagatg atcctcacca ccggagtctc gatcggagag
     8341 gacgcaaccg cgcagcggcg tttcgtccgc gacctcgaag ccgagggggc ggtaggcatc
     8401 gcggtcgaac tcgggttcgc ctggagccgg gagttgccca aggcgctggt tgacgaggcc
     8461 gaccggcgcc acatcccgat ggtcgccttt cgccgcggca tccggttcgt tgaggtttcc
     8521 gaagtcgtca acggcagcct gctcgactcc ggccatgccc tggcccgtcg cggcgaagaa
     8581 ctccaccgca gcctcgaccg gctggtgctg gaaggcgaag gggccgaatc ggttctggcc
     8641 gaggtgtcga ggaggatcgg gaacccggtc gtgctggagg acgcccgggg cgagctggtc
     8701 gcagtcggtt cggcaacccg cccggagagt gaagtggttg acacctggtc cggactgaaa
     8761 tggtcggacc gcaatcacgc cgaggcggag ggagccctgg ccgcgcaggt catggtccgc
     8821 ggccgcaact ggggccgggt gatcgcgctc cagaccgacg gggaatttga ccgcttcacg
     8881 ccgatcgccc tcgaaagagc ggcggtggcg gtcggtctgg gcctgatgag gggcggcgag
     8941 gaagccgaac tgcgggccag gtcgcgggga aacctgctct tcgagctggc ggcgggattg
     9001 atggaaccgc tggctgccgc acggcgcgcg tcaaccatgg gattcccccg gcggcatggc
     9061 cagctggtgc cgatcgccgc ggtctggcgg gccggggcac tcgaaaaggg cgtcggctac
     9121 gaggaggcgt gggcaccgat cctgccggcc gtgcgcgccg ccctggccga gcctgaccgt
     9181 cccgccctgc tcggctcacg gggcaactgc atgctcgggg tgcttgaccg gggaacactc
     9241 ggatcggagc ccgatgacgt cgcctcgcgc gagcgccttt cgcgacggtt cgccggggtc
     9301 ctggcccgcc acgagatcga tcccggatcg gtggcgctgg cgatcggggg tgccgccggc
     9361 agctgggaag agctggcgcc ggccctggtc cgggcccgct ttgccgccga ggccggggcg
     9421 gccgggccgc tgcgggcctg gcacgacgcg tcacggaccg agatcgggca gttgctcctt
     9481 tcgatgaggg gcacgccgga actgcttgag ttcagcgacg agcggctcgc gcccctgctc
     9541 gacctgccgg gggaaagggg tcgtgacctg ctcgacacgc tcgaggtcta cctgcacagt
     9601 ggcggacgga agaccgaggc cgcgaccgaa ctggagatcg agcggcagtc gctctatcac
     9661 cggctctcac gcatcgaaga ggtgcttgac gtggacctga aggatggcga cacggtgctc
     9721 gccctccacc tcgcgatcat gatcaaccgc ctgctctagg gcgggcttct agtcagccgc
     9781 gttcgcgacc tggatcccga tcacgacccc ggccgccgcc gcgatcagga agacggccag
     9841 gacggcgttt cgcctttccg gctgaatcgc cttgaccagc atcggtatcc cggccagggc
     9901 aggcagtccc atcagggcga aaagtatccc gcagacccac cagccggccg tgctgcccat
     9961 cccctccggc gggttgatcg cgtagtcgat gaagttgtag ccgagcgaga tgaagagggc
    10021 gggccagccc aggagcagca gcccggccgc ccccttcgcc gacttggcgc ctcccagcaa
    10081 ggcgatgaac aggccgatga tcccgatcgg aatgccggtg aacatcagca tttcggctcc
    10141 gtccgggcaa tgctggcgga ccacgtacgg cccgccctcg gcgcagtagc cgccgacgtc
    10201 catcacttcg cgcatcccga accagagggc ggtgatgccg taggcgacca tcaccatccc
    10261 gacgaagaag atgaccaacc cgaccaccgg gaagggctcg ttcttcggga tttgcgaaag
    10321 ccggtcggag agctccggct ggccggtcag gtcggacacg ggcggaagct tagctggccg
    10381 ctacctgggc ctggagctcc tcgaccacgg ccgggtcgcg cagggtgctt acgtccccga
    10441 gctccttgcc gtcggcgatg tcccggagca ggcggcgcat gatcttgccg gagcgggtct
    10501 tcggcaggtc atcggcgaag tagatcgcct tcggccgggc cagcttgccg atccggtcgg
    10561 ccacgtgctc cctgagttcg tcggtgaatt cgtgaccggg aggggcttcg tcattgggtg
    10621 tcgcgaaggc gatgatcgcc tggccggtga agtcgtccgg caccccgatc accgccgcct
    10681 cggccacatg cgggtgagaa accagcgagg actcgatctc catggtcgag agccggtgcc
    10741 cggagacgtt gatcacgtcg tcgatccggc cgacgatcca gaagtagccg tcctcgtcgc
    10801 gccgggccgc gtcaccgacc aggtaggtct ccttgccgta ccggtcccag taggcgttga
    10861 tgtagcgctc cggttccttg aaaagggtcc gggccatggc cggccagggc cgggtcagga
    10921 ccagggtgcc gccaccttcc tcgatctcgt tgccgttgtt gtcgaagatc gcggcacgga
    10981 atccgggcaa cggccgggtg gccgaccccg gcttcgtcgt ggtgatggcc ggcagggggg
    11041 agatcatcgc ggcgccggtt tcggtctgcc accaggtgtc gacgatcggg cagcggccac
    11101 cgccgatgtg ttcgttgtac cagagccagg ctttggggtt gatcggttcc ccgaccgttc
    11161 ccagcagccg gagcgaaccc aggtcgtgcg ctgccggcag ctcttcgccc cacttcatga
    11221 aggagcggat cacggtcggg gcggtgtaga ggatcgtcgc ccggtagcgc tcgacgatct
    11281 cccaccaccg atccttggtc gggaagtcgg gtgccccttc gaacatgatc gaggtggccc
    11341 cgttggcgag cggggcgtag acgatgtagg agtgaccggt gacccagccg atgtcggccg
    11401 cgcaccagta gacgtcgtcg tccttcaggt cgaagatcat gcggtgggtc gcggtggcct
    11461 gggtcaggta gccgccggtg gtgtggacga tgcccttcgg cttcgcggtc gaccccgagg
    11521 tgtagagcaa gaagagcgga tcctcggcgt ccatcggctc cggttcgcaa accgggtcgg
    11581 cggcagcgag ggcctctgcc cagtcaaggt cgcggccttc cttgagcgga gcgccggtcc
    11641 cgcagcgatc gaggacgatc acgtgctcca tcgccggcag cgtgtccaga gcctcgtcga
    11701 tcgctgcctt catcggcatc gggttgccgc ggcgcagggt ggcatcggcg gtgatcaggg
    11761 ccttcgcgtc cgagaactcc atccgttccc tcagggactc ggccgagaag ccgccgaaga
    11821 cgacgttgtg gatcgccccg atcctggtgc aggccagcat cgccacggcc gcctcgggca
    11881 ccatcggcat gaagatgccg accacgtcac ccttgccgac gccacggtcc ctgagcacgt
    11941 ttgcgagtcg ctgggtctgg tcgtggagcc atccgtaggt cacggagatc cgcccgcccg
    12001 cgtggctgcc gactccggtc accggctcgt cgtccgggcc ggcatcttcg gcctcccagt
    12061 ggtaggcgat gtcatctgcc cggcccgcct cgatgtggcg gtcgaggcag ttggcgctga
    12121 cgttgagctt gccgccggcg aaccaggtca cgtggggcgg gttgctccag tcgaggatcg
    12181 tgtcccaggg ttcgatccag tcgagcttct ccgcccagct cgcccaccag ccttcggggt
    12241 cggcgtcggc ctgctcgtag atcccagggt cattgaactc gctcgccgca gcgaaccctt
    12301 cggggggcgg gaaggtctgc tcggtcttca gaaggacgtc aagctctgac ttctctggca
    12361 ctgatctctc ctccggggtc ggggccggtt ctcctcggtg gcccaatgct agcccccgcc
    12421 ggactcccgg tccggaggat cggttcgggc actgcttcga ggcagcccgg aacggggcga
    12481 agggcgacgg tcagttgtcg ccgaaatgca ggacccagat ccgggccccg tggaaagact
    12541 ggaatgttcc cttgacgacg ccggtcccga cgtcggtgaa ggtccggctg aggatcgctt
    12601 cccgatgccc cttcgacttc atccaggcta tgaatatcga gcgcacgtcg ccgagcgatc
    12661 cgctgcccca ggcgatgttc tcgccggtcg cccagccgcc ttcgccgaga tagccggacc
    12721 gcttgatcca gaagtcaaag ctccttccgc aggcttcatg cgagaaaccg cagcgcagga
    12781 tgtccctggc cttgtggcgg gatgaccagg tcaactgctg ccgggtccgg taggggcgga
    12841 ggccttcccg gccccgggcg tagttgacca tgcagatcat cgacttgcgg gccttggcca
    12901 tcgcgacctt gccaccgctg gcggcgcggt tcgggcaggt cttggccggc gcgatcaggc
    12961 tggcggcatc ggccggtttg gctccgagct ggccgttgac gccaaccaga aggcaggctg
    13021 cgaccagaaa agcggcaagc aggcttgact gcttggctag tctggaaaca ggcgaaatga
    13081 aagagagctc aaacaatgtc tagtgattca acacctctca gcaggagtga tgcggagtgg
    13141 cgggaaagtc tttcccccga ggcctatgag gtcacccgca atgccgcgac cgaacggcct
    13201 ttcaccggcc ctcacctcga caacaagcag gacgggtcct acacctgcgt ctgctgcggg
    13261 gcagagctct tctcctccga cgcgaagttt gattcgggca gcggctggcc gagtttcacc
    13321 gaacccgcca acctcgaaca tgtcgagctg aggcaggaca acagtcacgg catggtgcgg
    13381 accgaggtcg tctgccgcaa ttgcgacgcc cacctcggcc acgtcttccc cgacggacca
    13441 gccccaaccg gccagcgcta ctgcatcaac gggtgtgctt tggaattcaa cgagaagtga
    13501 atgtgactga attccacccc ttgttccgat tcaacaagaa gtgaacgtga ctgaattcga
    13561 cccgttgttc cgatttaacg agcggtaggc ggcttttcgg ggtcccggtc caggaacgcg
    13621 tagcgggtcg cgatcgcgac cgcctgggtc cgggtgtcca ccccgagctt gttgacgacg
    13681 ttgcgcacat gcgtatagac ggtcgcggtc cggatgccga tttcctccgc cgctccctgg
    13741 gcccgcatcc cggttgccag cagggcgagg acttcccgct cccggtcgga gagttccatg
    13801 aagcgcttca gctcgtcttc ctgctgcagg tccttgtcgg tgtaaccggg gaaccaccgt
    13861 tcaccggcca tgacggtttc gcaggcgtcc ctgatctggt cgaggtcggc cgacttgacc
    13921 agaaaaccac tggcgccctc ccggctggcc aggcgcacca gactcgggtc gtcatgcgcc
    13981 gtgtagacga ggatcctgat ctcggggaag cgggagagga tccgccgcat cgactcgatg
    14041 ccgttgacgt tgggcatctc gatgtcgagc aggaccaggt ccggatggag tttcctgacc
    14101 gccggcttga cctcggcccc gtccccgacc tcctccacca cggtgaaccc ctcaaggtcg
    14161 tcgatgcgct ggcgaatcgc ctggcggacg atccagtggt catcacagat gaggaccctg
    14221 tgatctgtca attgccgccg atgactgcgg gagctccgta gctcacgagc gattccggaa
    14281 gccggaccga accgtcagcc tgctgatggt tctcaagcag ggcgatgatc gttcttccca
    14341 ccgcgaccgc cgtgccgttc agcgtgttca cgaattcggg cggtgccccg ggttccggcc
    14401 ggtatcggct gccgattcgc cgggcctgga agtcggtcgt gttcgaacat gaagtcagct
    14461 cgcggtacct gccctggctc gggatccagg cctcgcagtc gtacttcttg gccgccgaac
    14521 cgccgaggtc gccgaccgcc acgttgacca cccggtaggg cagcccgagt tcggtcagga
    14581 tcgattcctc gatcgccagg atccgctcgt gttcggctcc cgcgtcttcg ggcttgacga
    14641 aagagaacat ctctaccttg tcgaactggt ggacccggaa gatgccgtgg gtgtcccggc
    14701 ctgcggctcc cgcctcgcgg cggaagcagc ttgagaatcc ggcgtagcga agcggcagct
    14761 gatcggcttc gaggatctgg tcggcatgaa ggccggccag cggcacttcc gaggtgcccg
    14821 ccaggaagag ctcgtccttc tccagttcgt agatctgctc gcgatcgcct ggcagaaagc
    14881 cggtcccgaa cagggcttcc tcgcgaacca ggatcggcgg caccaccggc tcgtggcctt
    14941 cggcccgaag tttctccagc gcccagcgca ccagcgcgaa ttcgagcatg accagatccc
    15001 ccttgaggta ggcgaacctg gcaccggaga cttttgccgc cgcttccagc tcgatcgagt
    15061 cgccggcaag ttccacgtgg tccctgggct cgaagccgaa atccggaatc tcgccgacct
    15121 cgcggatcag ctcggcatct tcgtcggtgt atccgtcggg gctggtcggg tcgggcaggt
    15181 tgggcagcgt cgcagcaacc tcccggagct tgccgtcgac cgcttcaagc tcggcttcca
    15241 tttcctccag cctggccttc agctggcgca ccgcctccat ttgtgcggag gcgtcctcgc
    15301 cggccttctt cagcgcaccg atcttctcgg attcctgttt gcgctcgctg cgggcgcctt
    15361 cgatttcggg caggagctgt cgccggcgct cgtcgagcgt gagcagtgcg tcaacctggt
    15421 cggcggcgcc gcgccgggcc agggcagcct tgatccgctc gggatcggag cggatcagtt
    15481 tgaggtcgag cacggccccc gccgccggtt actggccgtc ggtcgggatc ggctcatcct
    15541 gcggggagga agcgtcttcc tctgccgagt aagcctcggc gcccgcctca ccggcgtcgg
    15601 acccggcgta ttcggcgacg aaccgggcga cttgctcggc ttcttccccg accacgatgt
    15661 tctgcggcat gatcgcgccg gagaatccac cgttctggat tgcgtgaagg gcgtcttcgt
    15721 aggtcacggc ccgctggtcg aggttcggac cctggttccg ggtcccgcgg ttgccggttc
    15781 cgagagcccc ggccggggtc agggtgtggc agccggcgca gttggcagcg aagagttgcg
    15841 ctccctcgta atccggggaa tccttcgaaa cggtgattcc ttcttcgcca aacccgcatg
    15901 cggacaggcc gagggcggcg gcgacggcaa gtgttgtgat ggctacggca cgcacagcga
    15961 gcgcatcata ttgtctgcgg cgaaacgatg gcagccccgg accgaaacga gtttcgccag
    16021 acggtgggtt tgttgccgac cggagtcacc gtggtctccg catttcaggc ctcgggaccg
    16081 gccggcgcga ccgcttccgc ggtctgttcg ctctcccttg agccgatgat gatgctggtc
    16141 tgcctcgacc tcggttcccg ctctctggag gcggttcgcg aggccggccg tttcgggatc
    16201 tcggtcctcg gcgagtcgca acgggccgcg gccgagatgt tcgcgacgaa ggcggaccag
    16261 gaagagaaat ggtcgtcggt cgtctggcgg gagcatctgg aggtgccgat catcgaggga
    16321 agcctggccc gggtggtctg cgacctggcc gaagtgattc cgggcggaga ccacgtgatc
    16381 gtgaccggcg aggtactcga agtcgaggcc gaggacgaac cgggggagcc gctgatctac
    16441 cacggcgggc tctttcgccg gctcgatgca caacaggatt gacgcacccg gtccggtttg
    16501 ccccggctag gcttggccga tgagcagcca cccctttttc tggctcgacg tcttcgccga
    16561 atccccgctg gcgggcaacg ggctcgccgt ggtcctcgac gcggacgggg tcagcgacga
    16621 gatcatgctc gccttcgccc gcgagaccgg tctctcggaa accaccttcg tccagtcgcc
    16681 ttcgtcggaa gcggcttcgt cgggcgccga ctaccgcaac cggatctgga cgatcgcgac
    16741 cgaggttccc ttcgccggtc acccttcgct cggtaccgcc gtggcggtcg cgctcgaccg
    16801 cggggtcgag gagatcacct tcacccagga gaccggggcc ggcctccagc aggtggaggt
    16861 cagccgggc
//
LOCUS       DAOQKN010000030        16863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_1545_length_16863_cov_5.701690, whole genome shotgun sequence.
ACCESSION   DAOQKN010000030 DAOQKN010000000
VERSION     DAOQKN010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 16863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16863
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1545_length_16863_cov_5.701690"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="PLJ64_03485"
     CDS             <1..597
                     /locus_tag="PLJ64_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNC92588.1"
                     /translation="HTMLAGVRDHHGFEVLGETLDDAAGEAIDKAARLLGLGFPGGPE
                     LERLAADGDPQAFTFPVAMSRDPGLDFSFSGLKTALVYISRELGEEETARRAADLAAG
                     YQAAVVDQLVTKLRRALEAGEWPAVALGGGVAANGPLRRAVAGLCEQKGIPFKEVNRE
                     LCTDNAAMIGSAARFSQPLAYPDYLSFDAFSTGPRGNR"
     gene            594..890
                     /locus_tag="PLJ64_03490"
     CDS             594..890
                     /locus_tag="PLJ64_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HNC92589.1"
                     /translation="MTGAGRTFEVTFLSRPGCHLCDHALAELNEYLAGRGGDGQAVPG
                     VIVEVINIDTDDELHRRFLERIPVILVGERIVSEFSFEPEALESVLGDWSQGAA"
     gene            912..1580
                     /locus_tag="PLJ64_03495"
     CDS             912..1580
                     /locus_tag="PLJ64_03495"
                     /inference="COORDINATES: protein motif:HMM:NF003995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HNC92590.1"
                     /translation="MSQTPKSRTDNPLLVGEGERLALGVAARLSRYLQVLIQARKMGR
                     ETISSQELSEYTHINPTQIRRDLSGFGKFGKRGVGYRIDYLIDEIRKIMRTAGQHNIA
                     LFGAGKLGQAIAGSDVFADHGFRIVSLFDVDPEMIGKEVGQLTVRDFAELGEAVQEEA
                     IVVGVLAVPSAAAQEVADGLVEAGVTVIFNYTEQLLQVPPKVTVHTSSPAVDLLYALY
                     FYLS"
     gene            1582..2736
                     /locus_tag="PLJ64_03500"
     CDS             1582..2736
                     /locus_tag="PLJ64_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HNC92591.1"
                     /translation="MSDHGLLEMDAVREVFENSIDFTIGLEEEFAIVDPDSLELVDRY
                     PDLYAAAQDDPVLAGSAAGELIASEIEIRSGRSETMSEATKRQEEKRRHLFRLAEAHS
                     VALASLGTHPWADYLDQEIIDTPHYQRLKKDLGWVARRNNTWSLHVHVGIRGADRAVA
                     ICDWLRERLPILLAASANSIFLDRQDTGLASVRTEIFTRTFPRCGIPGTFRDWTEYAR
                     FIDDLKAFGSVVEATQLWWSVRPHHAFGTVEVRICDAQTTAAESTGMAGLMTAVVAQT
                     AIDLDEGRLDGEEPLRGRQIEENLWRAIRYGMDGKMIDFRGRREIETRQLLEEVIEWT
                     GPARHKLGIEVELPDLNGAQRMRLDLESGKPIEEIYRETVAETAHSYAGP"
     gene            2746..3183
                     /locus_tag="PLJ64_03505"
     CDS             2746..3183
                     /locus_tag="PLJ64_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92592.1"
                     /translation="MTDEPLDQPPPNEPPQDEAGGPPPLTDEERAMLEAQMKEIRVED
                     LLTQTVASVLNLSARRIVKEDEVDLDQARIGIEAVRAIVALLPAEVGQAIAEPLSQVQ
                     LLYAQRSGETGEPGGEEGATGGAGPSNEPPAGHGPSKLWTPGS"
     gene            complement(3202..4317)
                     /locus_tag="PLJ64_03510"
     CDS             complement(3202..4317)
                     /locus_tag="PLJ64_03510"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNC92593.1"
                     /translation="MRNGRTVKYESLRIRPFGMHPAHRGYRDLGDYRRNQVRYGIAFA
                     TITAICFAATLVSSEDVQWGLYLGGWALALACVGLAWANPALWLLAPFGALGGAILVR
                     AGTDGLDAGIGPLLLIPMLAVAVYGSRRALIAMIACVAGAVIIGQLVWDGGNSEMSPI
                     WRQDIVLAMLATALGVAMQDLVTRMRSERNLAEERGRIIEELALTDPLTGIDNRRGWE
                     RMIQRSVGQARRHGQPLCLAMLDLDHFKLYNDTFGHQAGDDFLRRVALDWEERTRIED
                     HVARYGGEEFVVTLPNTGLAGAKVIVERLRTGLPLPLEDRESTITCSAGIAEWDGSET
                     VRELVARADQALYLAKEGGRDRTVTVEASARETGFRA"
     gene            4358..4930
                     /locus_tag="PLJ64_03515"
     CDS             4358..4930
                     /locus_tag="PLJ64_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92594.1"
                     /translation="MFKAPARADNIGSLVAKDRSKEILKEIRNLREELSARLDAIEAR
                     AGAGRFPESSSGFPDSAAPGASLPGIPVSNSGIPDPAPAVNDNFELPTGGEVRVIVRP
                     LHDLSLARVVEDALANTEGVNEASLRELRGDSATIDTKVDEGVSLISSLRRKLPVAFD
                     VTESSSDSVTIALAQPMGGRESGVAAPTGL"
     gene            4927..5184
                     /locus_tag="PLJ64_03520"
     CDS             4927..5184
                     /locus_tag="PLJ64_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92595.1"
                     /translation="MNSGGGVFGALIFVIGILLAFAVLFGFFLIVPFFVFLAGIVVML
                     VSDRRRGGENGNGNGNGNGNGTRTGTVEVVETTETVREVKS"
     gene            5184..7403
                     /locus_tag="PLJ64_03525"
     CDS             5184..7403
                     /locus_tag="PLJ64_03525"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNC92596.1"
                     /translation="MATAVAQKLLVRDSTSGVSERMVTLSSAVAHLLPVEICRRYHIV
                     PIEFDRGLITMAGPVSFNPAADRAVREQTGYETRWLFADQTELDLAIDRLFAGNGRPE
                     GTEFSADDLLRHGLAVPPRLGESLASKGLVTEEQLADAVAEQNRTGSRIGEVLVHGGA
                     LEETELLRVLSEQMDMPTVDLSDYDAERAPRDVLPDEMCNRLQCVPIAIDGESIYLAV
                     ADRLGEGEAEVESYTGRKVQQFLATRNDIARLQRQINRERYTETATLGLREQFPDSSA
                     HQVLSGGQKVFLVILLLVFLGFLIWKPIQTMVVVFAVLAIFYTLTSAYKLIAGYAALG
                     HRYLVDASPEALASMDERTLPVYTVLVPVYREAAVIPYLVAGIESLDYPKTKLDVRLL
                     CEEDDEETIQAIMDLDLPPHFNAIVVPPSQPQTKPKACNFGLLGAKGEYIVIYDAEDR
                     PDPLQLKKAVYMFDNVDDSIVCIQSKLNFFNQQTNILTRWFSIEYSMLFDLVLPGLDA
                     RRDPIPLGGTSNHIKRDELVEVGGWDPYNVTEDADLGVRLHQAGYRTTMMDSTTYEEA
                     NTSIPNWIRQRSRWIKGYLQTWLVYMRNPFRFLSNVGLKGFLSFQLLIGGTFMFLINP
                     IFWFLTTIFALTQLGIIEDFFPGWVYFLAASQLFFGNFVFVYLGLAASVRRGDYSLAP
                     YALFLPFYWGLMSVAAWKGFGQLVTNPFYWEKTEHGLDLGGGAEHAHTTTPRKAGTV"
     gene            7403..9352
                     /locus_tag="PLJ64_03530"
     CDS             7403..9352
                     /locus_tag="PLJ64_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92597.1"
                     /translation="MADERIDINPVIDDGGTAAVQEEVRTRRVRRTYVDPEVASSIGE
                     VPPEPRPFSPDAPRANWESGIVFAVFAIIYGVLGYFMLTDGRIVDFISLAHLNEAYMV
                     FWNDPPRLAAIGLDAATFSSIAYMPLSLVKPLATSLVAMPVLGAITAGLLMASLNSLM
                     RICEINLIFRIVLLILFGLNPMFAFFAANGDPETLGLCLLAIALSSTIAWSVTDQTRH
                     IAGAGLAMGLAVMVDYGYILVGLGFMVAIMIISASKNDDGLKTRSSLLLFLTPVAYAL
                     LFWCLMNWVLLGDPFQWVTAQNGVIQVNTTGALQAITTDVGGSLGDLFEVTLGVAPLA
                     LVAGFLLIIGSFLKKDATGWGLLVVGLCIAAAPVIRAVAADQADLLTISYGLPLAVFA
                     VGAVAWFGKDGWGVVAAVILGVGLIAAIPLSWNTMRDYRFQNQAEAFTRWVEHRDSQE
                     GTKSLGDYTVGIDPELSMARFINNKIPQEKRSILVDQNFSYGVMITSGRPQNFLDRVD
                     AGEDDWQATIDNPWGKDGERDVSYMLITTSREGDQIAKKWPTAVQGGEAGFTPIFRTD
                     RYVLVKVSETKPPSDESDQQSNEPRTTPNPVTPTSPLNPNGPDLTTPSQSETDVTPVS
                     PSPSNNGSTVEPNNGTSSAPSLEGE"
     gene            9352..10635
                     /locus_tag="PLJ64_03535"
     CDS             9352..10635
                     /locus_tag="PLJ64_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92598.1"
                     /translation="MPNRSMQRWILVIALTALLGLLVAACGSNDSDNGDSGDNSAKST
                     TGGTGATGDTAATANETTGATEPGTGSAVTAGTDPVAEGRLFSADSPWNTTAEGLPID
                     DASDRMLELAQFRRAAKEVPGQEGVQTFTRKIVDDGIFVNSQVWAPLIVAANTQDSVN
                     TKFVCRQSKCSSADPKVPSTLNIPANTTPDPRYDGWLSVIDRENGMGYDFWRARREGD
                     GTISYQFGKAWTLDGPGFSKPVSIDPDRAVGARGSGLPLFAGVIGPQELERGEISHAL
                     AISVPGLARRNFVQPASVTDGNGDRKSLPAGARIRLRADVSINEKKLRTLKGQRRRSA
                     DAILTALRTYGAIVVDRAAVPSLYAPLGTSPKLLRGDELDWLKLDDFEVISLPQIYKD
                     PPLSQVGLEGPETGGDNSQVQSGSAASGNEVGGGL"
     gene            10641..12062
                     /locus_tag="PLJ64_03540"
     CDS             10641..12062
                     /locus_tag="PLJ64_03540"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNC92599.1"
                     /translation="MLKNKRAGIACASAVLMLLVAGCGVNPDEAPESAGHDGSDANFV
                     PAQTGNSKTKEAKPTPREVGTIRIAGKAQGSLTPDLVRTYNAAGGTANVTVDEEGENQ
                     AFQQFCAGKADVVDSARPISEAEYRKCIANGIQPVQFQVASDAVVLAIKNETDVGTDC
                     LSFDEVRNIFRAGSDINSWSQVGYGHDLGGDTNEPRMKVAGPDEDSNVFGFFGQYVLG
                     APEPTMLDFRSDYQAFPTDAGVRRAVVGSNRDFRAAAQFQRSRQVYQGILDSLAENRR
                     AVRDARAEVAKGIADERPPEDQARDRRKLAKAQANLENLEKSLPAARRYARQNRAALR
                     RLAEVRGTLGLFRFSYYEAFEEQLRPMEVSASNDFAQPECIFPSQATVTNATYPLARQ
                     LLLTINYKNMTDQDINAFLVQSLSRSQQLAEDAALVPLPDETQATELAWLNGTSEPDI
                     IFYESGAGTSVGTQQEADSASGE"
     gene            12206..13750
                     /locus_tag="PLJ64_03545"
     CDS             12206..13750
                     /locus_tag="PLJ64_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92600.1"
                     /translation="MPAASGAAAKKKRVGPCSTLPINTKLSTHDPTGLLEDGYAWLYL
                     NPKGTVRSPNVKVVRHGRVFARGRIVGSLVPGRTTLVKMHVGRRMQQGKYRIRVTASK
                     PGRCSKRITKTRVWKFHRPTLGLKAIPVSTRIGDNVGVVRFALRPVRRTKVGWTRVSL
                     IGPGGGVVSQQVIKSIGSKQILAELPIKGKLKPAKYRVRLTGQDLASGIFRASSQTWR
                     FGKGGGGAKPVPTTGQLTQKVAVDWTNGKWDGRQVGGFIAPGIGYGEVVCSPYQQWVR
                     FYPSDTSRESAMMTWTYKNWDFGKGYEKAVREAKYTEGTGPDFREGMNKFGPTEKHST
                     GTFQGIISDRGPIDGPGGVALASPTTFDLDWVWDFNKEGSSYCHVKATFRTQTDQITD
                     PMARSVQIVWRGEANATAKNTSSVVDFPGLGNVQAICQAGPNGYRRLIVDSAIGGSVY
                     TREGSDDYKVNQGVGPLIMRLPNNGMLFIQMDNGDRILVSSRWKVNDPVASNNWCVVS
                     AQVYSP"
     gene            13845..14309
                     /locus_tag="PLJ64_03550"
     CDS             13845..14309
                     /locus_tag="PLJ64_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92601.1"
                     /translation="MSSESRHADAAAVDDLRASVGRVSLEVQALADRVQELVAAVESL
                     APRPPAAEHPVASAAPAQGEVVVSVSPLPELAMAAVAETALRGLGAVRKVASVERSND
                     QATFVLEVETEGDLITEMRRAMPVPITVDKAPDGSLKVVLEWAWGRSAASAG"
     gene            14321..16225
                     /locus_tag="PLJ64_03555"
     CDS             14321..16225
                     /locus_tag="PLJ64_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNC92602.1"
                     /translation="MAERIPRDQSLSRIYGSGALFAAAYGNVGSSIYYALGVTAAFAL
                     GLTPVAFMIAGLIFICTAATYAEATVMYPEAGGSSSFGRHAFNELVSFIAAWAQMLNY
                     TITVAISAYFVPHYLAVFWPALGHAPGDIVGGAIVIVFLAWLNIRGGQESARLNIILA
                     VADLATQVVLVGIGMFLVFNTNVLIENIHLGVAPTWPDFALGIAVGMIAYTGIETISN
                     MSEEAKDAARSVPQGVALVVVAVLALYLFIPIVALSAMPVHLDPSGNYVTELGTTFAD
                     DPILGIVENLGLSSGWTDILRVYVGVLAAVILVIATNAGLIGVSRLSYSMGQYRQLPE
                     VIRQIHPTYRTPYIAIIFFAILAIITLIPGQASFLATMYSFGAMLSFTIAHAAVIRLR
                     QKQPDAERAWEPRGNIRFRGALIPLTAVFGGFGTFAAWMVVMALNPVTLAVGTVWLAI
                     GLVGYMVFRRHQELPLTETVKVLLPEPLGVEEIEYKSVIVAFPADQPFSEEVMATAAK
                     LTSDRRRGIHITALLTVPTHLPLESSMAKEEKEAQSKIERAKLIAGLRVSGRVERVRP
                     GQEGYFLARQARTSKAAAVVIGLGSRGGVPQYGKILETVLRERPCRVIVVSEDPGVSD
                     PGVSREPVKA"
     gene            16222..16425
                     /locus_tag="PLJ64_03560"
     CDS             16222..16425
                     /locus_tag="PLJ64_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92603.1"
                     /translation="MKQGGVYDRSIRAFSIVYTVIGVIILAMTLVRGGGPLSIGFLIG
                     LAFIGVGVGRYVIQRRITGEGSE"
     gene            16437..>16863
                     /locus_tag="PLJ64_03565"
     CDS             16437..>16863
                     /locus_tag="PLJ64_03565"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNC92604.1"
                     /translation="MKIPEKAPQSGALWLLIVTYASVGFSIYFSLGVVAKKGLGLTPL
                     IFVATGVLFVLTIFTYFEGSAMLRERGGSSSFARHAFNELIAFVAGWVILLDYLIVIA
                     LAVLSVPHYLRPITGDIHHTVWVPIIIAAVVGYITVLNLL"
BASE COUNT         3015 a         5467 c         5318 g         3063 t
ORIGIN      
        1 cacaccatgc tggccggggt gcgggaccat cacggtttcg aggttctcgg ggaaaccctt
       61 gacgacgccg cgggcgaggc gatcgacaag gcggcccgcc tgctcggcct cggtttcccc
      121 ggcggccccg aactggaacg gctcgccgcc gacggcgacc cgcaagcctt cacctttccg
      181 gtcgccatga gccgggaccc ggggctggac ttcagcttca gcggcctgaa gacggcgctg
      241 gtctacatca gccgggaact gggggaggaa gagaccgcga ggcgggccgc cgatctggct
      301 gccggctacc aggctgccgt ggttgaccag ctggtgacca aactcaggcg cgccctggag
      361 gccggggaat ggccggcggt cgccctgggc ggtggtgtgg cggcaaacgg gccgctccgc
      421 cgcgcggtgg ccgggctgtg cgagcagaag gggatcccct tcaaggaggt gaaccgcgaa
      481 ctttgcaccg acaacgcggc gatgatcggc agcgccgcca ggttcagcca gccgctcgcc
      541 tatccggact acctctcttt cgacgctttc tcgaccggcc cccggggcaa tcgttgaccg
      601 gagcgggccg gactttcgaa gtcaccttcc tgagccgtcc cggctgccac ctttgtgatc
      661 acgccctggc cgagctgaac gagtatctcg ccggacgcgg cggggatggg caagctgttc
      721 ccggggtgat tgtcgaagtg atcaacatcg ataccgacga tgagctccat cgccggtttc
      781 tggaacggat cccggtgatc ctggtgggcg agcgcatcgt ctctgaattc agcttcgaac
      841 ctgaagcttt ggagtcagtc ctgggcgact ggtcacaggg cgctgcctga gacgggtacg
      901 cttgtagccc gatgtcacaa actcccaaat cccgcaccga caatccgctg ctggttggcg
      961 aaggggagcg cctcgctctc ggggtcgcgg cacggctttc gcgctacctg caggtgctga
     1021 tccaggcccg gaagatgggt cgcgagacca tctcctcgca ggaactctcc gagtacacgc
     1081 acatcaaccc gacccagatc cgccgcgacc tttccggctt cgggaaattc ggcaaacgcg
     1141 gggtggggta ccggatcgac tacctgattg acgagatccg caagatcatg cggacggccg
     1201 gccagcacaa catcgcgctg ttcggtgcgg gcaagctcgg tcaggcgatc gccggttcgg
     1261 acgtgttcgc cgatcacggc ttccgcatcg tttccctttt cgacgttgat ccggaaatga
     1321 tcggcaaaga ggtcggccag ctcacggttc gcgatttcgc cgagctcggt gaggcggtcc
     1381 aggaggaggc gatcgtggtc ggggtgctgg ccgtgccttc ggctgccgcc caggaggtcg
     1441 cagacggcct ggtcgaagca ggcgtgaccg tgatcttcaa ctacaccgag caactccttc
     1501 aggtgccgcc caaggtcacg gtccatacct caagcccggc ggtcgacctg ctttacgccc
     1561 tctacttcta tctctcctga tttgtccgac cacggcctgc tggaaatgga tgctgtgcgg
     1621 gaggtcttcg agaactcgat tgatttcacg atcggcctcg aggaagagtt cgcgatcgtc
     1681 gatccggaca gcctggaact ggtcgatcgc tatccggacc tctacgccgc ggcccaggat
     1741 gacccggtgc tggccgggtc cgccgccggg gagctgatcg cctcggagat cgaaatccgc
     1801 tcgggccggt ccgaaacgat gagcgaggcg accaaacggc aggaggagaa gcgccgccat
     1861 ctcttccggc tggccgaagc ccactcggtc gccctggctt cactcgggac ccacccctgg
     1921 gccgactatc tcgaccagga gatcatcgac accccccatt accagcggtt gaagaaggac
     1981 ctcggctggg tcgcccgccg caacaacacc tggagccttc acgttcacgt cgggattcgc
     2041 ggtgccgacc gggccgtcgc gatctgcgac tggctgcggg aacgactgcc gatcctgctg
     2101 gcggcctcgg ccaattcgat cttcctggac cggcaggaca ccggcctggc ttcggtccgt
     2161 accgagatct tcacccgcac ctttccccgc tgcggaatcc ccgggacttt cagggactgg
     2221 accgagtacg cgcggttcat cgatgacctg aaggccttcg ggtcggtcgt tgaagcgacg
     2281 cagctctggt ggagcgtccg tccgcatcac gctttcggca cggttgaagt acggatctgt
     2341 gacgcccaga cgacagccgc ggaatcgacc ggcatggccg gtctgatgac ggccgtcgtc
     2401 gcccagaccg cgatcgatct cgacgaagga aggctggatg gcgaagagcc gttgcggggc
     2461 cggcagatcg aggagaacct ctggcgggcg atccgttacg ggatggacgg aaagatgatc
     2521 gacttccgcg gccgccgcga aattgagacc cgccagctgc tcgaggaagt gatcgagtgg
     2581 accgggccgg cccgccacaa actcggcatc gaggtcgaac tgcccgatct gaacggcgcc
     2641 cagcgcatgc gcctggacct ggaatccgga aagccgatcg aggagatcta ccgtgagacc
     2701 gtggccgaaa ccgctcacag ctatgccggg ccatagagtg aagccatgac cgacgaaccg
     2761 ctggaccagc cgccgccgaa cgagcctccg caggatgagg cgggcgggcc accgccgctg
     2821 accgacgagg aacgggcaat gctcgaggcc cagatgaagg agatccgggt cgaggacctt
     2881 ctgacccaga ccgtggccag cgtgctcaac ctttcagccc ggcggatcgt caaggaggac
     2941 gaggtcgacc tcgaccaggc gcggatcggg atcgaggcag ttcgggcgat cgtcgccctg
     3001 cttccggccg aggtcggcca ggcgatcgcc gagccgctca gccaggttca gctgctctac
     3061 gcgcagcgga gcggcgaaac cggagagccc gggggagaag aaggcgcgac cggaggtgcc
     3121 ggcccgtcca acgagccgcc cgccggccat ggtccctcaa agctctggac tcccggcagc
     3181 tgaccggccg gccggaccag ctcaggcccg gaatccggtt tcgcgggccg aagcttctac
     3241 cgtgacggtc cggtcccggc cgccttcctt ggccaggtag agcgcctggt cggcccgggc
     3301 caccagttcc cgcaccgttt ccgacccatc ccactcggcg atgccggccg agcaggtgat
     3361 cgtgctctcg cggtcttcga gcgggagcgg caggccggtg cggagccgct cgacgattac
     3421 cttggccccg gccagcccgg tgttcggcag ggtcaccacg aactcctcgc cgccgtatcg
     3481 ggcgacgtgg tcttcgatcc tggtccgctc ttcccagtcg agggcgaccc ttctgaggaa
     3541 atcgtccccg gcctgatggc cgaaagtgtc gttgtaaagc ttgaagtggt cgaggtccag
     3601 cattgccagg caaagcggct ggccgtgccg ccgggcctga ccgaccgaac gctggatcat
     3661 ccgctcccag ccgcgccggt tgtcgatccc ggtcagggga tcggtcaacg ccagctcttc
     3721 gatgatccgg ccacgctcct cggcgaggtt gcgctcgctg cgcatacggg tgaccagatc
     3781 ctgcattgcc acaccgagtg ccgtcgcgag catcgccagc acgatgtcct gccgccagat
     3841 cggactcatc tcggagttgc cgccgtccca gacgagctgg ccgatgatca ccgcgccggc
     3901 cacacaggcg atcatcgcga tcagggcgcg ccgtgatccg tacacggcga ccgcgagcat
     3961 cgggatcagc agcagcggcc cgatgccggc gtcgagaccg tcggtccccg cccgcacgag
     4021 aatcgcaccg ccgagcgccc cgaacggcgc gagcagccag agggccggat tcgcccaggc
     4081 caggcccacg caggcaaggg cgagtgccca gccgcccagg tagagccccc actggacgtc
     4141 ctcagaggaa accagggtcg ctgcaaagca gattgcagtg atcgtggcga aggcgattcc
     4201 gtagcgaacc tgattgcgac ggtaatcccc gaggtcccgg tagccgcgat gggccgggtg
     4261 cattccgaac ggacggattc tcaggctttc gtacttgacg gttctcccgt tcctcaccta
     4321 tagtgggcca ctcactggcg gcaggactta ccattcggtg ttcaaagctc ctgcacgtgc
     4381 cgataatatc gggagcctgg tggctaaaga ccgttcaaaa gaaattctca aggaaatcag
     4441 gaatctaagg gaggaactct ccgcccggct cgacgccatc gaggcgcgcg ccggagccgg
     4501 tcgttttcct gaatcttctt ccggttttcc tgattcggcg gcgccaggcg cttcccttcc
     4561 cggaataccg gtgagcaact ccggcattcc ggatccggca cctgcggtca acgacaactt
     4621 cgagttgccg accggtggag aggtgcgggt gatcgtccgg ccgcttcacg atctgagcct
     4681 tgcccgcgtg gtcgaagacg cgctggccaa taccgaaggt gtgaacgagg cttcgctgcg
     4741 cgaacttcgc ggcgactcag ccaccatcga caccaaggtt gacgagggcg tttcgctgat
     4801 ctcttcgctt agacgcaaac tgccggtggc attcgacgtc accgagtctt ccagtgactc
     4861 ggtgactatc gcgctggccc aaccgatggg cgggcgcgag agcggcgtgg ccgctcccac
     4921 gggtctgtga acagcggcgg cggagttttc ggtgcgctga tcttcgtgat cggcattctg
     4981 cttgcttttg cggtgctgtt cggcttcttc ctgatcgtcc cgttcttcgt ctttttggcc
     5041 ggaatcgtcg tgatgctggt cagtgaccgg cggcgcggcg gtgaaaatgg caacgggaac
     5101 ggcaacggca acggaaacgg caccagaacc gggaccgtcg aggtggtcga aaccaccgag
     5161 acggttcggg aggtcaagtc ctgatggcca ccgccgtcgc acagaaactg ctggtccgcg
     5221 attccaccag cggcgtcagc gagcgcatgg tcacgctcag ctcggccgtg gcacatctgt
     5281 tgccggtcga gatctgccgc cggtaccaca tagttccgat cgaatttgac cgcggactga
     5341 tcaccatggc cgggccggtt tccttcaatc cggcggccga ccgggcggtg cgcgagcaga
     5401 ccggttacga aacccgctgg ctcttcgccg accagaccga gctcgacctg gccatcgacc
     5461 ggcttttcgc cggcaacgga cggccggaag ggaccgagtt ctcggccgat gacctgctcc
     5521 ggcacggact ggccgttccg ccccggctgg gcgagagcct ggccagcaag ggcctggtca
     5581 ccgaggagca gctggccgat gccgtggccg agcagaaccg caccggaagc cggattggtg
     5641 aagtactggt ccacggcggc gcgctagagg aaaccgagct gctccgcgtg ctctccgagc
     5701 agatggacat gccgaccgtc gatctcagcg attacgacgc cgaacgggcg ccgcgcgacg
     5761 tgctgccgga cgagatgtgc aaccggctcc agtgcgttcc gatcgcgatc gacggcgaga
     5821 gcatctacct cgcggtcgcc gaccggctgg gtgagggtga agctgaggtt gagagttaca
     5881 ccgggcgcaa ggttcagcag tttctcgcca cgcgcaatga catcgcccgg ctgcagcgcc
     5941 agataaaccg ggagcgctac acggagaccg ccaccctcgg cctccgggag cagttcccgg
     6001 acagctcggc ccatcaggtg ctgagcggag gccagaaggt cttcctcgtg attttgctgc
     6061 tggttttcct cggattcctg atctggaagc cgattcagac catggtcgtg gtcttcgcgg
     6121 tgctcgcgat cttctacacg ctgacctcgg cctacaagct gatcgccggt tacgccgcgc
     6181 tcggtcaccg ttaccttgtt gacgccagtc ccgaggcgct ggcctcgatg gatgaacgca
     6241 ctctccccgt ctacaccgtc ctcgtgccgg tctaccgcga ggctgccgtc atcccctacc
     6301 tggtcgcggg tatcgagtct ctcgactacc cgaagaccaa gctcgacgtg cgtctgctct
     6361 gcgaggaaga cgatgaggaa acgattcagg cgatcatgga cctggatctg ccgcctcact
     6421 tcaacgcgat cgtcgttccc ccgtcacagc cgcagaccaa gccgaaagcc tgcaacttcg
     6481 gcctgctcgg tgccaagggc gaatacatcg tcatctacga cgccgaggac cgcccggatc
     6541 cgctccagtt gaagaaggcc gtctacatgt tcgacaacgt ggacgactcc atcgtctgca
     6601 ttcagtcgaa gctcaacttc ttcaaccagc agaccaatat cctcactcgc tggttctcga
     6661 tcgagtactc gatgctcttc gacctggttc tgcccgggct cgacgcccgc cgcgatccga
     6721 tcccgctcgg tggcacctcg aaccacatca agcgggacga acttgtcgaa gtggggggct
     6781 gggacccgta caacgtgacc gaggacgcgg acctcggcgt ccgtctccat caggccggat
     6841 accgcaccac aatgatggat tcgaccactt acgaagaggc gaataccagc atcccgaact
     6901 ggatccgcca gcgctcccgc tggatcaagg gctacctgca gacctggctc gtctacatgc
     6961 ggaatccgtt ccgcttcctc tccaacgtcg gcctcaaggg attcctttca ttccaactgc
     7021 tgatcggcgg aactttcatg ttcctgatca acccgatctt ctggttcctg accaccatct
     7081 tcgcgctgac ccagctcggc atcatcgagg acttcttccc cggctgggtg tacttcctcg
     7141 ccgcgtcaca gctcttcttt ggcaacttcg tcttcgttta cctggggctc gcggcttcgg
     7201 tccggcgtgg tgattacagc ctcgcgccct acgcgctctt cctgccgttc tactggggcc
     7261 tgatgtctgt cgcggcctgg aagggcttcg gtcagctcgt aaccaacccg ttctactggg
     7321 agaagactga acacggactc gacctcggcg gcggtgccga acatgcccac acgacgactc
     7381 cgcggaaagc gggcaccgtc tgatggccga cgagcggatc gacattaatc ccgtaattga
     7441 cgacgggggt accgccgcgg ttcaggaaga agtcaggacc agaagggtcc gtcgcaccta
     7501 tgtcgaccct gaggtcgcgt caagcatcgg tgaagtcccg cccgaaccgc ggcctttcag
     7561 cccggacgcg cccagggcca actgggagag cggcatcgtc ttcgcggtct tcgcgatcat
     7621 ctacggcgtc ctcggctact tcatgcttac cgacgggcgc atcgtcgatt tcatttcgct
     7681 ggcgcacctc aacgaggcct acatggtctt ctggaacgac cctccgcgcc tggcggctat
     7741 cggcctggat gccgcgacct tcagttcgat cgcctacatg ccgctcagtc tggtcaagcc
     7801 gctggcgacc tcgctggtcg cgatgccggt tctgggggca attacggccg gtttgctgat
     7861 ggcatctttg aactcgctga tgcggatttg cgagatcaac ctgatcttcc gcatcgttct
     7921 gctgatcctg ttcggcctga atccgatgtt cgcgttcttc gccgccaacg gcgacccgga
     7981 gaccctcggt ctgtgcctgc tcgccatcgc actctcatcg acgatcgcct ggtcggtcac
     8041 tgaccagacc cgtcacatcg ctggtgccgg tctggccatg gggctggccg taatggtcga
     8101 ttacgggtac atcctcgtcg gactgggctt catggtggcg atcatgatca tcagcgccag
     8161 caagaacgat gacggactga agacccgctc cagcctgctg ctcttcctga ccccggtcgc
     8221 ctacgcgttg ctcttctggt gcctgatgaa ctgggtgctc ctgggtgacc cgttccagtg
     8281 ggtgaccgcc cagaacgggg tcatccaggt caacaccacg ggggccctgc aggcgatcac
     8341 caccgatgta gggggcagtc tcggagacct gtttgaagtt acccttgggg tggcgccttt
     8401 ggctctggta gcgggattcc tgctgataat cggctcgttc ctgaagaagg acgcgaccgg
     8461 ctggggcctg ctggtagtcg ggctctgcat cgctgccgcg ccggtgatcc gggccgtcgc
     8521 cgccgaccag gcggatctgc tcaccatttc ctacggtctg ccgcttgcgg tgttcgcggt
     8581 tggtgcggtg gcctggttcg gcaaggatgg ctggggggtc gtcgctgcag taatcctcgg
     8641 ggtcgggctg atcgcggcga taccgctgag ctggaacacg atgcgggatt accggttcca
     8701 gaaccaggcg gaggcattta cccgctgggt cgagcatcgc gacagccagg agggcaccaa
     8761 gtctctgggc gactacacgg tcggcatcga tccggaactc tccatggcca gattcatcaa
     8821 caacaagatt ccccaggaga aacgatcgat cctcgttgat cagaacttca gctacggcgt
     8881 gatgatcacc tccgggcgtc cccagaactt cctcgaccgg gtcgacgcgg gtgaagatga
     8941 ctggcaggcg acgattgaca acccctgggg caaggacggc gagcgggacg tcagttacat
     9001 gctgatcacc accagccgcg aaggcgatca gatcgccaag aagtggccta ccgccgtcca
     9061 gggcggagaa gcggggttca ccccgatatt ccgaactgac cgctacgtgc tggtgaaagt
     9121 ctccgagacc aagccgcctt cggatgagag cgatcagcag tcgaatgagc cgcgaacgac
     9181 tccgaatccg gtcaccccga cttcgcccct gaatccgaac ggtccggatc tgaccacacc
     9241 gtcccagtct gaaacggatg tgactccggt ttcgccatcg ccgtccaata acggcagcac
     9301 ggttgaaccg aacaacggta cgtcctccgc cccgagcctg gagggtgagt agtgccgaat
     9361 cgcagcatgc agcgctggat cctcgtgatc gcgttgacgg ccctgctcgg cctgctggtc
     9421 gctgcctgcg gttcgaatga ttcggacaat ggtgattccg gcgacaactc ggccaagtcg
     9481 actaccggcg gcaccggagc aactggcgat accgctgcga ccgcgaacga aacgactggt
     9541 gcaaccgagc ccggcaccgg ttccgcggtg accgctggca ccgatccggt cgccgagggc
     9601 cgccttttct cggccgacag tccctggaac acgacggccg agggtctgcc gattgatgat
     9661 gcctccgacc ggatgctgga actagcccaa ttccgaaggg ctgcgaagga ggttcccggc
     9721 caggaaggcg ttcagacctt cactcgaaag atcgtcgacg acggcatctt cgtcaactcg
     9781 caggtctggg ctcccctgat cgtcgcagcc aacacccagg attcggtcaa caccaagttc
     9841 gtctgccgtc agtcgaagtg cagctcggct gatcccaagg ttccgagcac gctgaacatc
     9901 cccgcgaaca ccactcccga cccgcgctac gacggctggc tcagcgtgat cgaccgggaa
     9961 aacggtatgg gatatgactt ctggcgtgcc cgccgtgaag gcgacggcac catttcctac
    10021 cagttcggaa aggcctggac cctggatggc cccggcttca gcaagccggt cagcatcgac
    10081 ccggaccggg cggttggtgc gcgtggatcc gggctgccgc tcttcgcggg tgttatcggc
    10141 ccccaggaac tggagcgggg cgagatcagc catgccctgg cgatttcagt tcccggcctc
    10201 gcccgtcgca attttgttca gcccgcttcg gttactgacg gcaacggcga tcgcaagtcg
    10261 ctgccggccg gggcccggat ccgtttgcgc gcagacgtgt cgatcaacga gaagaagctc
    10321 cgcaccctga aggggcagcg tcgacgctcg gccgacgcga tcctgaccgc gctcaggacc
    10381 tacggcgcaa tcgtggttga tcgggccgcg gtgcccagcc tctacgcccc gctcggtacc
    10441 tcgccgaagc tgctccgggg tgatgaactc gactggctga agctggacga ttttgaagtg
    10501 atttcgctgc ctcagatcta caaggatccg ccactaagcc aggtcggcct cgaaggtccc
    10561 gagaccggcg gtgacaattc gcaggttcag tccggcagcg ccgcgtctgg caacgaagtc
    10621 ggaggtggac tgtgagtcgg gtgctgaaaa acaagagggc cggtatcgcc tgcgcatcag
    10681 ccgtgctgat gctgcttgtc gccggctgtg gcgtgaatcc ggatgaagca cctgagtcgg
    10741 cgggtcacga cggctccgac gcgaacttcg tgccggccca gaccggcaac agcaagacca
    10801 aggaagccaa acccaccccg cgtgaggtcg ggaccatccg gatagccggc aaggcccagg
    10861 gttccctgac ccccgatctg gtcaggacct acaacgcggc tggtggcacc gcaaacgtga
    10921 ccgtcgacga agaaggcgag aaccaggcct tccagcagtt ctgcgccggc aaggccgatg
    10981 tcgtcgattc ggcccggcca atctccgaag ccgaataccg caaatgcatc gccaacggca
    11041 ttcagccggt ccagttccag gtcgcctcgg acgcggtcgt ccttgcgatc aagaacgaga
    11101 ccgatgtcgg caccgactgc ctcagcttcg atgaagtccg gaacatcttc cgggccggtt
    11161 ccgacatcaa cagctggagc caggtcggtt acggccacga tctcggcggc gacacgaacg
    11221 aaccgaggat gaaggtcgcc ggaccggacg aggattcaaa cgtcttcggc ttcttcggcc
    11281 agtacgtgct cggggctcca gaaccgacca tgctcgattt ccgcagcgac taccaggcct
    11341 tcccgaccga cgccggggtg cggcgtgcgg tcgtaggcag caaccgggac ttccgggcgg
    11401 ccgcccagtt ccagcgctcg cggcaggtct accaggggat cctcgattcg ctggccgaga
    11461 accgccgcgc ggtgcgtgac gcccgggccg aagtcgccaa gggaatcgcc gacgaacgcc
    11521 cgccagagga tcaggcccgc gatcgccgca agctggccaa ggcccaggca aatctcgaga
    11581 acctcgagaa gtccctgcct gcagcccgcc gctatgcgag gcagaatcgc gccgccctcc
    11641 gtcgcctggc ggaagtccgc ggaacgctcg ggctcttccg cttctcctac tacgaggcat
    11701 tcgaagagca gctgcggccg atggaagtca gcgcatcgaa cgatttcgcc cagccggaat
    11761 gcatcttccc ctcgcaggct acggttacca acgcgaccta cccgctggcc cggcaactgc
    11821 tcctgaccat caattacaag aacatgactg accaggacat caacgccttc ctggtccaga
    11881 gtctctctcg ttcccagcag cttgctgaag acgcagccct cgtgccgctg ccggatgaga
    11941 ctcaggcgac cgagcttgcc tggctgaacg gcacctccga gccggacata attttctacg
    12001 aatctggcgc aggaacctcg gtcggaacgc agcaagaggc cgattcggcc agtggcgagt
    12061 agccgcaatt cctcacagat ccacgcggat tttccgctac tcggccttga acgcgggttt
    12121 aaggcggggt gagatcatga ttaacgtgtt tcggcgtata gcgacaatcc tcttgctcat
    12181 cggtctgggt tttaccttgg ccgcgatgcc tgctgcttcc ggcgcggccg cgaaaaagaa
    12241 gcgggtcggt ccctgctcga ccttgccgat caacaccaag ctgagtaccc atgacccgac
    12301 cgggctactg gaggacggtt atgcctggct gtacctgaac cccaagggca ccgtccgctc
    12361 gccgaacgtc aaggttgtac ggcatggccg cgtgtttgcc agaggccgga tcgtcggcag
    12421 tctcgtaccg ggtcgcacca cgctggtcaa aatgcatgtt ggccgtcgca tgcagcaggg
    12481 caagtatcgg atcagggtga ccgccagcaa gccggggcgc tgcagcaagc gaatcaccaa
    12541 gacccgcgtc tggaaatttc accggcctac cctcgggctg aaggcgattc cggtcagtac
    12601 ccggatcggt gacaacgtcg gcgtcgtccg cttcgccctg cgaccggtcc gccgaaccaa
    12661 ggtcggctgg accagggttt cgctgatcgg tcccggcggt ggcgttgtca gccagcaggt
    12721 gatcaagtcg atcggcagca agcagatcct ggccgagctg ccgatcaaag gcaagctgaa
    12781 gccggccaag taccgggtcc gcctgaccgg gcaggatctg gcttccggca tcttccgcgc
    12841 ctcgagtcag acctggcgct tcggcaaggg cggcggtggc gcgaagccgg tgccgaccac
    12901 aggccagctg acccagaagg tcgccgtcga ctggaccaac ggcaagtggg acggccggca
    12961 ggtcggtggt ttcatcgcgc ccggcatcgg atacggcgaa gtcgtctgca gcccgtacca
    13021 gcagtgggtg cgcttctacc cctctgatac cagccgcgag agcgcgatga tgacctggac
    13081 ctacaagaac tgggacttcg gcaagggcta cgaaaaggcc gtgcgtgaag ccaagtacac
    13141 cgaaggaacc gggcccgact tccgcgaggg catgaacaag ttcggtccga ccgagaagca
    13201 ttcgaccggc accttccagg ggatcatctc cgaccgcggt ccgatcgacg gaccgggcgg
    13261 cgtggccctg gcatcaccga ccaccttcga tcttgactgg gtctgggatt tcaacaagga
    13321 aggcagctcc tactgccacg tgaaggcgac cttccgcacc cagaccgatc agatcaccga
    13381 tccgatggcc cgttcggtcc agatcgtctg gcggggtgag gccaacgcga ccgcaaagaa
    13441 cacttcgagc gtcgtcgact tccccggact cgggaacgtg caggcgatct gccaggccgg
    13501 tccgaacggt taccgtcgcc tgatcgtgga ttcggcgatc ggcggcagcg tctacacccg
    13561 ggaaggttcc gacgactaca aagtcaacca gggcgtcggt ccgttgatca tgcgcctgcc
    13621 caacaacggc atgctcttca ttcagatgga caacggagac cggatcctgg tctcttcgag
    13681 gtggaaggtc aacgacccgg ttgcctccaa caactggtgc gtggtttccg cccaggtcta
    13741 ttcaccttaa cggcgcccgg tcttcgccgg gcaccaacgg aagcccgccc gcccacagcg
    13801 gcaactgatt gatgggcggg ctctggtgga gataggatgg gaccgtgagt tcagaatccc
    13861 gacatgcgga cgccgccgcc gtcgatgacc tccgtgcttc ggtaggtcga gtcagccttg
    13921 aagttcaggc gttggccgat cgggttcagg aactcgtggc cgcggttgaa tctcttgccc
    13981 ctcgccctcc ggcagcagag catcccgtcg cttcggctgc cccggcacaa ggcgaggtag
    14041 tagtcagcgt ttcccccttg ccggaactgg caatggccgc ggtcgccgag acggcgcttc
    14101 gcggactcgg tgctgttcgc aaagttgcgt ccgtggaacg atcgaatgat caggcgacgt
    14161 tcgtgctcga ggtcgaaacg gaaggcgatt tgatcaccga gatgcgccgg gccatgccgg
    14221 tgccgatcac cgtcgataaa gcccccgacg gatcgttgaa agtggttctg gaatgggcct
    14281 ggggaaggtc tgccgcaagc gcaggctgag ttgatgagtc ttggctgaac ggattccgcg
    14341 tgaccagagc ctgagccgca tctacggttc aggagccctc ttcgctgccg cctacggcaa
    14401 tgtcggctcg tcgatctatt acgcgctcgg cgtcaccgcc gcctttgcac tcggccttac
    14461 cccggtcgcc ttcatgattg ccgggctgat cttcatctgt accgcggcca cttacgccga
    14521 ggcgaccgtg atgtacccgg aggccggtgg ttcgtcttcc ttcggccggc acgccttcaa
    14581 cgagctggtc agcttcatcg cggcctgggc gcagatgctg aactacacga tcacggtcgc
    14641 gatctcggcc tacttcgtgc cgcattacct ggccgtcttc tggccggcgc tcgggcatgc
    14701 accgggcgac atcgtcggcg gggcgatcgt gatcgtcttc ctcgcctggc tcaacatccg
    14761 gggcgggcag gagtcggcgc ggctgaacat catcctcgcc gtggccgacc tggccaccca
    14821 ggtggttctg gtcggaatcg gcatgttcct ggtcttcaac acgaatgtcc tgattgaaaa
    14881 catccacctc ggggtcgccc cgacctggcc tgatttcgcg ctgggaattg cggtcggcat
    14941 gatcgcctac accggcatcg agacgatctc gaacatgtcg gaggaggcca aagacgcggc
    15001 cagatcggtc ccgcagggag tggcactggt cgtcgttgca gtgctcgcgc tctatctgtt
    15061 cattccgatc gtggcccttt cggccatgcc ggttcacctg gatccgtccg gaaactacgt
    15121 gaccgagctg ggcacgacct tcgccgacga tccgatcctc ggaatcgtgg agaacctcgg
    15181 gttatccagc ggctggaccg acatcctcag ggtctatgtg ggtgtcctcg cggcagtcat
    15241 cctggtgatc gcgaccaacg ccggactgat cggggtatcc cggctcagtt attcgatggg
    15301 ccagtaccgg cagttgcctg aggtcatccg ccagatccac ccgacctacc gaacgcccta
    15361 catcgcgatt attttcttcg cgatcctcgc gatcatcacc ttgatcccgg gtcaggcttc
    15421 cttcctcgcg accatgtact ccttcggcgc gatgctgtca ttcacgatcg ctcatgccgc
    15481 ggtgatccga ttaaggcaga aacagccgga tgcggaacgc gcctgggagc cacgggggaa
    15541 catccgattc cgcggtgccc tgatcccgct caccgcggtg ttcggtggct tcggcacctt
    15601 cgccgcctgg atggtggtca tggcactcaa cccggtgacc ctggccgtcg ggaccgtctg
    15661 gctggcgatc gggctggtcg ggtacatggt cttccgccgc catcaggaac tgcccctgac
    15721 cgagaccgtg aaggtgctct tgcccgaacc gctcggggtg gaggagatcg agtacaagtc
    15781 ggtgatcgtc gcctttccgg ccgatcaacc cttctccgaa gaggtgatgg cgacggcggc
    15841 aaagctgacc tcggatcgcc ggagggggat ccacatcacc gccctgctca cggtgcccac
    15901 ccacctgccg ctcgagagtt cgatggcgaa agaggagaag gaggcccaga gcaagatcga
    15961 gcgagcgaag ctgatcgcag gcctgcgggt ttcgggccgg gttgaacggg tccgccccgg
    16021 acaggagggc tattttctcg cgcgccaggc ccgcacctca aaggcggcgg cagtcgtgat
    16081 cggtctggga tcaaggggcg gggtgccgca gtacggcaag atcctcgaga cggtgcttcg
    16141 ggagcgtccc tgccgggtga tcgtggtctc cgaagatccc ggcgtgagcg atcccggggt
    16201 ttcccgtgaa ccggtgaagg catgaagcag ggcggggtct acgaccgctc gattcgggcg
    16261 ttctcgatcg tctacacggt gatcggcgtg atcatcctcg ccatgaccct ggtccgtggc
    16321 ggcgggccac tttcaatcgg attcctgatc gggctcgcct tcatcggagt cggcgtcggc
    16381 cggtacgtga tccagaggcg gatcaccgga gagggatccg aatgaagatg cgggacgtga
    16441 aaattcccga gaaggcgccg cagtcggggg cactctggct cctgatcgtc acctacgcct
    16501 cggtcggctt ctcgatctac ttctcgctgg gggtggtcgc gaagaagggc ctggggctga
    16561 ccccgctgat cttcgttgcg accggggtgc tcttcgtgct cacgatcttc acctacttcg
    16621 aaggcagcgc gatgctgcgc gaacggggcg gttcctcgtc gtttgcaagg catgccttca
    16681 acgaactgat cgccttcgtc gcaggctggg tgatcctgct cgactacctg atcgtgatcg
    16741 ccctggcggt gctgtcggtg ccgcattacc tgcggccgat caccggcgac atccaccaca
    16801 ccgtctgggt gccgataatc atcgcggcgg tcgtcggcta catcaccgtc ctcaacctgc
    16861 tcg
//
LOCUS       DAOQKN010000031        16717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_1573_length_16717_cov_6.546153, whole genome shotgun sequence.
ACCESSION   DAOQKN010000031 DAOQKN010000000
VERSION     DAOQKN010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 16717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16717
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1573_length_16717_cov_6.546153"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..382
                     /locus_tag="PLJ64_03570"
     CDS             107..382
                     /locus_tag="PLJ64_03570"
                     /inference="COORDINATES: protein motif:HMM:NF024769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HNC92605.1"
                     /translation="MSTGVQKLPGGVVHKLPADLRSGLIKHEVALAAWKDITPLARNE
                     FICWVEDAKKQETRERRIRRTREELEEGMRRPCCWPGCDHRERNGKA"
     gene            436..1428
                     /locus_tag="PLJ64_03575"
     CDS             436..1428
                     /locus_tag="PLJ64_03575"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNC92606.1"
                     /translation="MEDGEQERAGRSRRVWVSEICWFLTIFFMLWAIVRTGGLERGFP
                     LVQLLAYTPYVLLLSLIALLIVVLCRRWLAAGFLLIACLALALAVLPREIGGPETVPG
                     GQPVRVMTINLGVGYAHADEIAELARVRDVDLVAVQELTPAEASGLDRAGFATDYPHR
                     ILQPGTEASGGGIYSRWPLVDRGSLTAEFHQPVADVRIPASIPIEFVSVHPMAPTTPA
                     RTSQWASEYKSLPEASSARLPLVLAGDFNATLDHRNLRDLIDTGYRDAAEVTGSGLVT
                     TWPSRIEWKLPVTLDHVLAEKGISFSQYDVEKVAGTDHRAVIVELVMPPVPGGR"
     gene            complement(1447..1770)
                     /locus_tag="PLJ64_03580"
     CDS             complement(1447..1770)
                     /locus_tag="PLJ64_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92607.1"
                     /translation="MNPSTALKSGLVMLALVAVLAVLLAVALPKSFFEDWGWFSGPAA
                     LFLCAFGTARILGLPVGRTLLGVFIAGIPGLIGVVTGVHWLGTLLAVLTFAVWCGYGP
                     EPRRA"
     gene            1827..3059
                     /locus_tag="PLJ64_03585"
     CDS             1827..3059
                     /locus_tag="PLJ64_03585"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNC92608.1"
                     /translation="MTRSSGIFRSGDRLRQQVSLLFFFLLIAGLAILIPSHASSADQN
                     PIRSSRLSQKGSSLIVQIRTSQKVNLGRLDRRPDFANGNHRYLCLQMNPRGGTTIVRI
                     CLGGRSRTYRVLGVSRTSEAGRVRSSKVIRAEVKRVGQLKLVATFDPAAAGLPPGNYA
                     WRIADHSGECPPSGPKARPTEECLSYFPPKKRAVYRLRPIRVVGCTGGNGELVRNGPR
                     GGKRVALTFDDGPSDYTPEVLHILKRKEVKATFFMLGQQVTRYPSYARRVLALGHEIG
                     NHSYDHALLPVSSDIRHTNRSIIQATHFRPCLFRPPYGAVSSSLVASARAARMKVVDW
                     DVDTTDWKLPGAGYIKSNVLGNAHSGSIVLMHDGGGPRSQTVEALPGIIDGLRGRGYR
                     LVTVSQLLGNRMIYRPVP"
     gene            complement(3120..5576)
                     /locus_tag="PLJ64_03590"
     CDS             complement(3120..5576)
                     /locus_tag="PLJ64_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92609.1"
                     /translation="MRRYLVLLAALLAGMFIVGGVADAAGKQVKPARPSVKILTPASS
                     QLAKGTVRIQVKANKGAKVRLSAIARSFDTPNQRLFTNRTLYLRKKAGMAISMSLTPE
                     AKATARTCQALTLIITAKNGKKTTRVTRSVNRDLGDCQLSPVDLSRAADCDFIAEPKE
                     GMCMLPFPDDYYTVEDESSPTGKRIHFTDAGMPVNAVNEPISAASYDLSDGFSQGQGI
                     VVKIPGVESQAAVDANDFVPINHIGQYADKNQKAVVINADTGERHPIWVNIDANAGSD
                     QDRVLEIKPAVNFDEKGHYIVALRNLVTAAGKPIAAPTAFRYYRDQIRSSQKPINDRR
                     AHFEQMFETLKQAGIKRRDLYLAWDFTTASNENNYKRALSMRNRAFAELGDTNLGDQI
                     VQGSAPQFTIDSVQVLPTGDIARKIKGHYTVPCFLEPSCGPGGTMNLDANGLPSRNGN
                     YTANMECIIPRVAVQPGAEKTRPMVFGHGLFGDASGVNGGPNPPLAQKGMTICGTDEI
                     GMSNSDVVQVMTSALQNLSHFNVLADRLAQGLINELFLARLMYHPNGLGTAPAFQDGD
                     GFTAGQSLIKTDHVYYMGASQGGIMGGPLTALSPDFTQSSLLVGSMNYSVLLPRSIDF
                     DLYSAILYPSYPGDLAHPLLFQLMQMLWDRSEPNGYAHVMTDNPPPDTPEHKVTLMIA
                     VGDHQVSNYASEVMARTLGMSTNQTPVDEGRWPDYDILWNVPRLSASDYPFHGNNVIY
                     FDGGPPRPEPGNPSKTIGTDVPPFANVPNRSAQDPHGAPGGASVAVEMTSTFLQPNGY
                     ISDVCSPHACYGDAWDGSLP"
     gene            5748..6962
                     /locus_tag="PLJ64_03595"
     CDS             5748..6962
                     /locus_tag="PLJ64_03595"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HNC92610.1"
                     /translation="MTSTFRRRFGLMLLLLPVLFTLSQPDPGKANAGLTIPSTTTRAA
                     TAPFNKAAMWIWYIDDSNGGNIGSIIRQARRAHIGTLYIKSGDGVDAWSQFNRPVVRR
                     FHRAGLKVCGWQYIYGRRPIAEAKISAVAKRRGADCFVIDAESEFEGAYAAADWYIRS
                     LRQRVGRRFPIGLAGFPYANYHPGYPYSVFLGPGAANANVPQVYWHTIGDSVRTALEI
                     TWEQNSIYKRPIFPVGQTYEDPGARELLAYRRFMLNYGTRPSWWSWQETSPAEWKILA
                     RPVSRVSGYRAYSGHVTLEKGDRGDQVLWLQQHLIAAGYSMEPTGIFSRSTYKAVRRF
                     QARRGLPGDGVVGTRTWNRLMRIRPVRVRWSAAHRRNKAAGASASSAPLSAPLSADLP
                     PVRNEIAGAKHP"
     gene            complement(6968..7816)
                     /locus_tag="PLJ64_03600"
     CDS             complement(6968..7816)
                     /locus_tag="PLJ64_03600"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNC92611.1"
                     /translation="MRLVTYASERGPRAGVQTDQGVIDAWDLVDGEGASLRELIASGR
                     LDEVRTALDGGGGEPVMLSPGGILAPVPDPDKIICVGLNYRSHAEETGMELPKAPTIF
                     GKYRNALVPDGAKVTLPAASSKCDYEAEVAFVIGRRAREVSVEDALDHIAGYTLLNDL
                     SARDLQGLTMQWMAGKVFDGSAPCGPALVTPDEAGPHDGIGIRMLVNGEERQSSNTSD
                     LIFSVPQLVSTLSSWMTLEPGDIVATGTPSGVGLAMDPRTWLEDGDELVVSSPQLGEL
                     HTTIAR"
     gene            7985..8058
                     /locus_tag="PLJ64_03605"
     tRNA            7985..8058
                     /locus_tag="PLJ64_03605"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8019..8021,aa:Asp,seq:gtc)
     gene            complement(8060..8551)
                     /locus_tag="PLJ64_03610"
     CDS             complement(8060..8551)
                     /locus_tag="PLJ64_03610"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNC92612.1"
                     /translation="MSPEPSGEVERTAGGRDLILRRDLPLEPDQAWAWITESGKTGQW
                     FGTWTGAGEAGGEIEVTMVAEEGNPKSAMKINACEPGRSYEVTGSDESGGWNLELKLE
                     EGEGGTRLTFIHHLGEEDKVEMIGPGWEFYLDHLLAAIEGNEKKDFSEYWPAMGPYYA
                     ELG"
     gene            complement(8606..10126)
                     /locus_tag="PLJ64_03615"
     CDS             complement(8606..10126)
                     /locus_tag="PLJ64_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC92613.1"
                     /translation="MDQRSRPKSDERIDPDDLETALRVIGQLDRLPAEHPDSVTIQQA
                     AAGLYKSVKKRRKLEKRRQVLEHDAEITARTATAAPGRIDDETEGLPLVSSAKGAIAG
                     TLIEARACYICKQPFHQVDAFYHQLCPDCAAFNHARRDARTDLTGKRALLTGGRAKIG
                     MYIALRLLRDGADTTITTRFPNDAVRRFRAMKDSEEWFDRLHIVGIDLRDPGQVVDLA
                     DSIAAGGPLDILINNACQTVRRTPDAYAPLIRAEAEELEPGPVPDIETFARHGEHHLQ
                     PAHLEDGNSRWVGNPSGEPEIRPTPNAASHWAPTPHAIAAMALTAGSADFDLVDSGRA
                     IDAGGLVPDVVDTNSWIQRVNEVDPIELLEVQLCNMTAPFILISRLRQAMADSPARRT
                     YVVNVSAMEGQFSRGYKGPGHPHTNMAKAALNMLTRTSAQEMLEADGILMTAVDTGWI
                     TDERPHPMKKRLADDGFHAPLDLVDGAARVYDPIIRGEEGEDIHGVFLKDYKPSPW"
     gene            10178..10741
                     /locus_tag="PLJ64_03620"
     CDS             10178..10741
                     /locus_tag="PLJ64_03620"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNC92614.1"
                     /translation="MTKLLIVIASVRPGRVGESVANWFVPIAEADERFEVEVADLKEL
                     DLPFMDEPNMPGRREYTKEHTKKWSAIVDRADAFVFVTPEYNATPAPALKNAIDYLYH
                     EWSYKPCSFVSYGGVWAGARAVQALKPTLTQLSVMPVPEMVPIPGVSGLLDDQGNLQG
                     TEIHEKSAGGMLNSLEKWSGALKTLRQ"
     gene            complement(10825..13158)
                     /locus_tag="PLJ64_03625"
     CDS             complement(10825..13158)
                     /locus_tag="PLJ64_03625"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNC92615.1"
                     /translation="MIPGIRFAETRRHLAFAFALAAFVCCLAFGASRATAAEPAVFEV
                     GAASSSINPDSPQYVGGYGYKQGPTQTTHDNLEARAFVVGKGKDALAFVSVDLVGWFA
                     AYDGEQAPYGIDATREKIADALNSRGYDIGRESVIVSSTHVHAAPTVVGIWGTLDGEY
                     LKKVSDAAVTAASEAADAARPSEIWTGVGDIRSFIWQNGQGTNHPDGFEYDNALPILW
                     ARDPDTGATNALYANVPNHPDQYQVDDNNFEMSADWPGYARRKLDALNGGTAVLAAGT
                     LGRQEPPGSVTAYSEVEAQGEIVANEIQRTMAKSTPLTDDTIAASEQFMETPADNDDL
                     LLGISIFTYPGACIDGIERCTIPRSKAPPYMNEVSGEDVIGTYVEAARIGDVAFATNP
                     GEAFPEVNFAIRDGVTGPRQVNVIAQAGDMLGYYYQRADYTDQQFGSSDFEDYNVGPE
                     LGQANANSALAGLNEIGFTATPETVHAAFESSVASKPGIQWYPDEYESTDPTFNILGS
                     AARSQDGTAPVPTSISWDFGDTTTGTTSVGERFDHTFPGPGSYEVTATVSSNGLTRSW
                     TDTITVDPPLTADASVAKRTGQGASLLGGALGGSGKIVAAHWTCQDGKKVSGLKVLCK
                     SPKAGKASVTVVDGAGNTAKATVPVAKAPPASLTIRKISVKPTKIKYGKKGTMTVAVK
                     NPSTTNATGFKVCVKVPARAKKQIKAMPACKSPGNLRAGKSASVSIRIQVTRQASPGM
                     KVKLPVETSANGAKKKTASISILGKPGGAVSSSYAAG"
     gene            complement(13288..13821)
                     /locus_tag="PLJ64_03630"
     CDS             complement(13288..13821)
                     /locus_tag="PLJ64_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HNC92616.1"
                     /translation="MAKKKSFAKALNEQISNEFAASQQYVAMAVYYDSETLPRLAAFF
                     YRQALEEREHAMMMIQYLLDVDEEVEVPDVKSQPTKYEDGITPVKEALAQEERVSSEI
                     FGLFELAREIKDYRAEHFMQWFVKEQVEEVALMGDLLNVVERSKDNLLLAEEYIARES
                     FGEEGNDPTAPPPAGGN"
     gene            complement(13917..14984)
                     /locus_tag="PLJ64_03635"
     CDS             complement(13917..14984)
                     /locus_tag="PLJ64_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92617.1"
                     /translation="MSTYERVAELPLKIESWSMEGLSIKPSPEFERLTTVIRLEGAGQ
                     TGLGEDITYEALDQIALQDAGATHDLTGPNTVGEFCELIGGLDLFPSPPEREVSRLYR
                     RWAFESAALDLALWQAGTSLAGYLDRPLNPLTFVASMGLAGGPGGTKSDVGRLRVKLD
                     RYPDLKFKLDPSLDWDDALIGELVETGAVNTLDLKGQYKGTVVDIETDPELYRRLVLA
                     FPDAWIEDPDLTPETREVLKDDWHRVTWDAPIHSVADIAELEHKPTVINIKPSRMGGL
                     KSTLDAYDHCEAEGIGAYGGGQWELGVGRDQIQYLAALFHPDNPNDTAPVGYNAAEPE
                     AGLPVSPMKVPGNGFGKPDQS"
     gene            15021..15413
                     /locus_tag="PLJ64_03640"
     CDS             15021..15413
                     /locus_tag="PLJ64_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNC92618.1"
                     /translation="MSSLIHTCYRVFDLDESIDFYKKLGLLEHHRLPIGDEAVNVYMG
                     HEGDGARLELTFNYDQQEPYEIGTGYGHIAFVVDDLDSTLEGLADQGIAPEKPPYTVS
                     EGGSRLCFVKDPDGYRIELIEGDPGRAD"
     gene            complement(15410..15889)
                     /locus_tag="PLJ64_03645"
     CDS             complement(15410..15889)
                     /locus_tag="PLJ64_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WHG domain-containing protein"
                     /protein_id="HNC92619.1"
                     /translation="MSRRAEITAAARSILEQEGEEALTMRNLADRLGIKAPSLYKHVS
                     SRREIETLLAAEALNEMRESLAAADGLEEMGRSYRAWAIANPSLYRIATTRPLDRENL
                     PTGLEDAAAAPLLAAVDGDRARARAFWALAHGLVLLELDGRFPGDADLEAAWLAGIS"
     gene            complement(15886..16362)
                     /locus_tag="PLJ64_03650"
     CDS             complement(15886..16362)
                     /locus_tag="PLJ64_03650"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNC92620.1"
                     /translation="MPSIHTTELIDASPDRVWQILADLSSYPDWNPFIIEASGELREG
                     EKLVLRMQPPGGKAMNFRPTVLKADRGQELRWLGRLGFRGIFDGEHYFRLTPEGGGTR
                     LDQGEEFTGLLPRFMGKVLSQTERGFEALNAALRKRAERDVEAPAGLAPEKETAGS"
     gene            complement(16433..>16717)
                     /locus_tag="PLJ64_03655"
     CDS             complement(16433..>16717)
                     /locus_tag="PLJ64_03655"
                     /inference="COORDINATES: protein motif:HMM:NF024744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HNC92621.1"
                     /translation="TLLFLGVDEEDVYRDYLLTNEQLLPALKPILSHIEEAGIEFDLL
                     RPVIGVDRSYLETAVDLMKERYGSIDGYMRDGLGLEDGTLLALRRRLLDT"
BASE COUNT         2686 a         5377 c         5594 g         3060 t
ORIGIN      
        1 agagcgcctg ctttacacgc aggatgccgg gggttcaagt ccctcaccgc ccatctcctg
       61 cccgggctgt tcgaggtctc attgacccat cacctacgat ggaggcatga gcaccggcgt
      121 tcagaagttg cccggcgggg tggtgcacaa gctgcccgcc gacctgcgta gcgggttgat
      181 caaacatgaa gtggcgcttg cggcgtggaa ggacatcacg ccgctggccc gcaacgagtt
      241 catctgctgg gtcgaggatg cgaagaagca ggaaacccgg gaacggcgga tccgccggac
      301 cagggaagag ctcgaggaag gcatgcgccg gccctgctgc tggcccggct gcgaccatcg
      361 cgagcgcaac ggcaaggctt agccccgaat cccggatcgg gctgaaccat ccgcgatgtc
      421 gggataattc ccggcttgga agacggcgag caagagcgag cgggccggag ccggcgggtc
      481 tgggtctcgg agatctgctg gtttttgacc atcttcttca tgctctgggc catcgtccgt
      541 accggtggtc tggaacgcgg cttcccgctg gttcagcttc tcgcctacac gccgtacgtt
      601 ctgctgctct cgctgatcgc cctcctgatc gtcgtgctct gccgacgctg gctggcggcg
      661 gggttcctgc tgatcgcctg cctggctctc gccctggccg tcctgccgcg ggagatcggc
      721 gggccggaga ccgtcccggg cggtcaaccg gtgcgggtga tgacgatcaa cctcggggtc
      781 gggtacgccc acgccgatga aatcgccgag ctcgcccggg tccgggacgt cgacctcgtc
      841 gcggttcagg aactgacccc ggcagaagcc tccgggctcg accgggccgg cttcgcgacc
      901 gactatcccc accggatcct tcagccgggg accgaagcgt ccggcggagg catctactcc
      961 cgctggccgc tggtcgaccg gggaagtctg accgccgagt tccaccagcc ggtggccgac
     1021 gtgcggattc ccgcctctat cccgatcgaa ttcgtctcgg ttcacccgat ggccccgacc
     1081 acgccggccc ggacctcgca gtgggcctcc gagtacaagt cattgccgga agcctccagc
     1141 gccaggctgc ccctggttct ggccggggac ttcaacgcga ctctcgatca caggaacctt
     1201 cgcgacctga tcgacaccgg ctaccgcgac gccgccgagg tgaccggcag cggcctggtt
     1261 acgacctggc cgtcgcggat cgaatggaaa ctgcccgtga ccctcgacca tgtgctggcc
     1321 gaaaagggca tttcgttcag tcagtacgac gtggagaagg tcgcgggcac cgatcaccgg
     1381 gccgtgatcg tcgagctggt catgcctccg gtgcccggcg gccggtgagc gccggcacct
     1441 gaaccttcag gcgcggcggg gttccgggcc gtagccgcac cagacggcga aggtgagcac
     1501 ggcgagcagg gtgccgagcc agtggactcc cgtgaccacg ccgatcaggc cggggatgcc
     1561 ggcgatgaac accccgagca gcgtgcggcc aaccggcagg ccgaggatcc gggccgtgcc
     1621 gaaggcgcag agaaacagtg cggccgggcc ggagaaccag ccccagtcct cgaagaatga
     1681 cttcggcagc gccacggcga gcagcaccgc cagcaccgcc accagggcga gcatgacgag
     1741 gccggacttg agggcggttg acggattcat gacggcagac ggccgactcg ggaaggccac
     1801 gccggagcct atactgggag cgcatcatga cccggtcgtc aggcatattt cggtcggggg
     1861 acaggcttcg ccaacaggtc tcactgctgt tcttcttcct gctcatcgca gggctggcga
     1921 ttctcatccc ttctcacgcc agcagcgccg accagaaccc gattcgctct tccaggctgt
     1981 cccagaaggg gagctcgctg atcgtccaga tcaggaccag ccagaaggtg aacctgggca
     2041 ggcttgaccg ccggcccgac ttcgcgaacg gcaaccaccg ctatctctgc ctgcagatga
     2101 atccccgcgg cgggaccacg atcgtgcgga tctgcctcgg cgggcgaagc cgcacctacc
     2161 gggtgcttgg cgtctcacgg acttcggagg ccggccgggt gcgttcgagc aaggtgatcc
     2221 gggcagaagt gaaaagggtc gggcagctga aactcgtcgc cactttcgac ccggcggctg
     2281 ccggtctgcc tcccgggaac tatgcgtggc ggatcgccga ccattccggc gagtgcccgc
     2341 cgtccgggcc gaaggcgagg ccgaccgaag aatgcctttc gtacttcccc ccgaaaaagc
     2401 gtgcggtcta ccggcttcgg ccgatccggg tggtcggctg cacgggtggc aacggcgagc
     2461 tggtgcgaaa cggcccgcgc ggcggcaagc gagtggccct gaccttcgat gacggtccga
     2521 gcgactacac cccggaagtc cttcacatcc tcaagcgcaa ggaagtcaag gcgaccttct
     2581 tcatgctggg ccagcaggta accaggtatc cgtcatacgc ccgacgggtt ctggcgctgg
     2641 gccacgagat cggcaatcac agctatgacc acgccctgct tccggtcagc tccgacatcc
     2701 gtcacaccaa ccggagcatc atccaggcga cccacttccg gccctgcctg ttccgaccgc
     2761 cctacggcgc ggtcagcagc tcgctcgtgg cctcggcccg ggcggcccgg atgaaggttg
     2821 tcgactggga tgtcgacaca accgactgga agctgcccgg tgccgggtac atcaagtcga
     2881 acgtcctcgg taacgcgcat tcagggtcga tcgtcctgat gcatgatggc ggcggccccc
     2941 gcagccagac ggtggaggcg ctgcccggaa tcatcgacgg gctccgcggg cggggttacc
     3001 ggctggtcac ggtctcgcag ctcctcggca accggatgat ctaccgcccg gtcccctgaa
     3061 aagcgaaagc cccgggcgtt tccgcaccgg ggctttctct cggaatccgt ttctgccggt
     3121 tacggcaggc tgccgtccca ggcgtcgccg tagcaggcgt ggggtgagca gacgtcactg
     3181 atgtagccgt tcggctgcag gaaggtcgag gtcatttcga ccgcgacgct ggccccgccg
     3241 ggagctccgt gcggatcctg ggccgaacgg ttcggaacgt tggcgaaggg gggcacgtcg
     3301 gtgccgatcg tcttcgacgg attgcccggc tccggtcgcg gcggtccacc atcgaagtag
     3361 atgacgttgt tgccgtggaa cggatagtcg gacgcggaca gcctcgggac gttccagagg
     3421 atgtcgtaat cgggccagcg tccttcatcg accggcgtct ggttggtgct catgccgagg
     3481 gtccgggcca tcacttccga ggcgtagttg gagacctgat ggtcgccgac cgcgatcatc
     3541 agggtgacct tgtgctcggg ggtgtccggc ggcgggttgt cggtcatcac gtgggcgtaa
     3601 ccgttgggct cgctgcggtc ccagagcatc tgcatcagct ggaacaggag cggatgggcc
     3661 agatcgcccg ggtaggacgg gtagaggatc gccgagtaga ggtcaaagtc gattgaacgc
     3721 ggcagcagca ccgagtagtt catcgaaccg accagcagcg aggactgggt gaagtcgggc
     3781 gagagcgcgg tcagcggacc gcccatgatc ccgccctggc tggcgcccat gtagtagacg
     3841 tgatcggtct tgatcagcga ctgtccggcc gtgaaaccgt caccgtcctg gaaggccgga
     3901 gcggtgccga ggccattcgg gtggtacatc aggcgggcca ggaacagctc gttgatcagg
     3961 ccctgggcca gccggtcggc gagcacgttg aagtgcgaca ggttctgcag ggcgctggtc
     4021 atcacctgga ccacatccga gttggacatg ccgatctcgt cggtgccgca gatcgtcatg
     4081 cccttctggg ccagcggcgg gttggggccg ccgttgacgc cggaggcatc accgaacagg
     4141 ccgtggccga agaccatcgg ccgggtcttt tcggcaccgg gctggaccgc gacccgggga
     4201 atgatgcatt ccatgttcgc cgtgtagttg ccattgcgcg agggcaggcc gttggcgtcg
     4261 agattcatgg ttccgccggg accgcaggac ggttcaagga agcagggaac cgtgtagtgg
     4321 cccttgatct tgcgggcgat gtccccggtg ggaagcacct ggacgctgtc gatcgtgaac
     4381 tgcggagccg agccctggac gatctggtcg ccgaggttcg tgtcaccgag ttcggcgaag
     4441 gcacggttgc gcatgctcag cgcccgcttg tagttgttct cgttgctggc ggtggtgaag
     4501 tcccaggcaa ggtagaggtc ccggcgcttg attccggcct gcttcaaggt ctcgaacatc
     4561 tgctcgaaat gagcccggcg atcattgatc ggcttctggc tggagcggat ctgatcccgg
     4621 tagtagcgga aggcggtcgg cgccgcgatc ggcttgccgg cggcagtgac gaggttgcgc
     4681 agggcgacga tgtagtggcc cttctcgtcg aagttgacgg cgggcttgat ctccagcacc
     4741 cggtcctggt cggaaccggc gttggcgtcg atgttgaccc agatcggatg gcgctcgccg
     4801 gtgtcggcgt tgatcacgac cgccttctgg ttcttgtcgg cgtactgacc gatgtggttg
     4861 atcggcacga agtcattcgc gtcaaccgcg gcctgggact cgacccccgg gatcttcacg
     4921 acgatgccct ggccctggct gaacccgtcc gaaaggtcgt agctggccgc cgagatcggc
     4981 tcgttcacag cgttaaccgg catgccggcg tcggtgaaat ggattcgctt gccggtcggg
     5041 ctggactcgt cctcgaccgt gtagtaatcg tcggggaagg gcagcatgca catgccttcc
     5101 ttcggttcgg cgatgaagtc gcagtcggca gcccggctca gatcgaccgg gctgagctga
     5161 cagtcgccga gatcccggtt gaccgacctc gtgaccctgg tcgtcttctt cccgttcttc
     5221 gcagtgatga tcagggtcag ggcctgacag gtccgcgcgg tggcctttgc ctccggagtc
     5281 aggctcatgc tgatcgccat gcccgccttc ttgcgcaggt agagcgtgcg gttcgtgaag
     5341 agacgctggt tgggcgtgtc gaaggatctg gcgattgccg agaggcgcac tttcgccccc
     5401 ttgttcgctt tgacctgaat cctgacggtg cccttggcga gctggcttga cgccggggtc
     5461 aggatcttga ccgaaggcct ggctggcttc acctgcttgc cggcagcgtc tgcaactccg
     5521 ccgacaatga acattccggc aagcagagcc gccagcagaa cgaggtaacg acgcatcctt
     5581 tgattcctcc caaacagagt ttgaacttct ccctgccccg caggctacag ggcagggagg
     5641 tgaaatgaaa gaaagcttgc ttgcgggtcc gaggccgccg cttgccgggg gttctcctgc
     5701 gaggctgaat ccgctccgat cagccatcaa ccgtgttcca gaccctgatg acatcgactt
     5761 tccgccggcg cttcgggctg atgctcctgc tgctgccggt cctgttcacg ctttcccagc
     5821 cggatcccgg caaggccaac gccggcctca cgatcccgtc cacgacgacg agggcggcga
     5881 ccgctccctt caacaaggca gcgatgtgga tctggtacat cgacgattcc aacggtggca
     5941 acatcggttc gatcatccgc caggcccggc gggcccacat cggcacgctc tacattaagt
     6001 ccggtgacgg cgtcgacgcc tggagccagt tcaaccggcc cgtcgtccgg cgttttcacc
     6061 gggccgggct caaggtctgc ggctggcagt acatctacgg caggcggccg atcgccgaag
     6121 cgaagatctc ggccgtcgcc aagcggcgcg gggcggactg cttcgtgatc gacgccgagt
     6181 ccgaattcga aggcgcctac gctgccgccg actggtacat ccgttccctt cgccaacggg
     6241 tcggacgccg tttcccgatc gggctcgcag gcttcccgta cgccaactac cacccgggct
     6301 acccctattc ggtctttctc ggcccggggg cggcaaatgc gaatgtgccg caggtctact
     6361 ggcacacgat cggggattcg gtgcggaccg cgctggagat cacctgggag cagaacagca
     6421 tctacaagcg gccgatcttc ccggttggcc agacctacga ggatcccggt gcccgggagc
     6481 tgctcgccta ccgccgcttc atgctcaact acggcacccg gccgagctgg tggtcctggc
     6541 aggaaaccag cccggccgaa tggaagatcc tcgccaggcc ggtgagccgg gtgtccggtt
     6601 accgggccta ctcgggtcat gtgacgcttg agaagggaga tcgcggtgac caggtgctct
     6661 ggctgcagca gcatctgatc gcggccggct actcgatgga gccgaccggg atcttcagtc
     6721 gcagcaccta caaggcagtc cgccggttcc aggccaggcg gggtctgccg ggcgacggcg
     6781 tggtcggaac ccggacctgg aatcgcctga tgcggatccg tccggtcagg gtcaggtggt
     6841 cagctgccca ccgccgcaac aaggccgccg gggcgtccgc ttcatcggca ccgctcagcg
     6901 caccgctctc cgccgatctg cccccggtcc gaaacgaaat cgccggcgcc aagcacccct
     6961 gagcctgcta gcgggcgatc gtggtgtgaa gctcgcccag ctgtggcgag ctgacgacca
     7021 gttcgtctcc gtcttcaagc caggttcgcg ggtccatcgc caggccaacc ccggaagggg
     7081 ttccggtggc gacgatgtcg cccggctcca gggtcatcca gctcgaaagg gtcgagacca
     7141 gttgcgggac gctgaagatc aggtcggagg tgttcgagct ctggcgctcc tcgccgttga
     7201 ccagcattct gatgccgatc ccgtcatgcg gcccggcctc gtccggagtg accagggcgg
     7261 ggccgcaggg agccgagccg tcgaagacct tgccggccat ccactgcatg gtcaggccct
     7321 gcaggtcgcg ggccgaaagg tcgttgagaa gggtgtagcc ggcgatatgg tcgagcgcat
     7381 cttcgacgct gacctctctt gcccgccggc cgatcacaaa ggcgacctct gcctcgtaat
     7441 cacacttgct gctcgcggct ggcagggtca ccttcgctcc gtccgggacc agggcattgc
     7501 ggtacttgcc gaagatggtc ggggccttcg gcagttccat gccggtctcc tcggcgtgcg
     7561 agcggtagtt cagcccgacg cagatgatct tgtccggatc gggaaccggc gcgagtattc
     7621 cgcccgggct cagcatcacg ggttcgccac cgcccccgtc cagcgcggtc cggacctcgt
     7681 cgaggcggcc ggaggcaatc agttccctga ggcttgcgcc ctcgccgtca accaggtccc
     7741 aggcgtcgat cacgccttgg tcggtctgga ctccagcccg cgggccccgc tccgatgcat
     7801 acgtgaccag tctcatggcg cgagtctaga acccgaacgc cggaaacgat cggcaaccgg
     7861 gggcgattga ccacacaaac ggtgggtaaa cgcccccgga acgggaatcg ggaagggtca
     7921 gatgtcgccg ttcgttcgag actgcgataa tgcccgcccg gtcggtttcg accgatcgcc
     7981 tcacgggggc gtagctcagt tggttagagc gctggcctgt cacgccagag gccgcgggtt
     8041 caagtcccgt cgctcccgtt catccaagct ccgcgtagta cggccccata gcgggccagt
     8101 actcgctgaa gtccttcttc tcgtttccct cgattgccgc cagcaggtgg tcgagataga
     8161 actcccagcc cggcccgatc atctcgactt tgtcttcctc accgaggtgg tggatgaagg
     8221 tcagcctggt cccgccttcc ccttcctcca gcttcagttc caggttccag ccgccggact
     8281 catccgagcc ggtcacctcg taggagcggc ccggctcgca ggcgttgatc ttcatcgccg
     8341 acttcgggtt gccctcctcg gcgaccatgg tcacctcgat ttcaccgccg gcttcccccg
     8401 cgccggtcca ggtaccgaac cactggccgg tcttccccga ctcggtgatc caggcccagg
     8461 cctgatcagg ttcgagcggc aggtcccgcc gcaggatcag gtcgcggccg cccgcggttc
     8521 gttcgacttc tccggaaggt tcggggctca tgaccgcagg ttctcaagtc agggcagcga
     8581 tgtccacctc gcccgcaacc ggaggtcacc agggcgaagg cttgtagtcc ttgaggaaga
     8641 ctccgtggat gtcttcgcct tcttcgccgc ggatgatcgg gtcgtagacc cgggcggcgc
     8701 cgtcgactag gtcgagcggg gcgtggaagc cgtcatcggc caggcgcttc ttcattgggt
     8761 gggggcgttc gtcggtgatc cagcccgtgt cgaccgcggt catcaggatg ccgtcggcct
     8821 cgagcatctc ctgggcgctg gtccgggtca gcatgttgag cgccgccttg gccatgttgg
     8881 tgtggggatg gccggggccc ttgtagccac gggagaactg gccttccatc gccgagacgt
     8941 tgaccacgta ggtccgtctg gccggggagt cggccatcgc ctggcgcagc cggctgatca
     9001 ggatgaaggg tgcggtcatg ttgcagagct ggacctcgag cagctcgatc gggtcgacct
     9061 cgttgacccg ctggatccag ctgttcgtgt caaccacatc ggggacaagt ccaccggcgt
     9121 cgattgcccg gccggaatca accaggtcga agtcagccga accggcggtc agtgccatcg
     9181 ccgcaatcgc atgcggtgtc ggagcccagt gcgaggccgc attcggggtg ggtcggattt
     9241 ccggttcccc tgacggattt ccgacccacc gggaattgcc gtcctcgagg tgggccggtt
     9301 gaaggtgatg ttcgccgtgg cgggcgaaag tctcgatgtc cgggaccggg ccaggttcga
     9361 gctcctcggc ttcggcccgg atcagcgggg cgtaggcatc cggtgtgcgg cggaccgtct
     9421 ggcaggcgtt gttgatcagg atgtcgagcg gtcccccggc cgcgatcgag tcggcgaggt
     9481 cgaccacctg ccccgggtcg cgcaggtcga tcccgacgat gtggagacgg tcaaaccact
     9541 cttcgctgtc cttcatcgcc cggaaccggc gcaccgcgtc gttggggaaa cgggtggtga
     9601 tcgtggtgtc ggcgccgtca cgaagcaggc gcagggcgat gtacatcccg atcttcgccc
     9661 ggccgccggt caggagcgcc cgcttgccgg tcaggtcggt gcgggcgtca cgacgggcgt
     9721 ggttgaaggc ggcgcagtcg gggcagagct gatggtagaa ggcgtcaacc tggtggaagg
     9781 gctgcttgca gatgtaacag gcacgggcct cgatcagggt gccggcgatg gcgcccttcg
     9841 cgctggagac gagcggcagc ccttccgtct cgtcgtcgat ccgccccggt gcggcggtgg
     9901 ccgtccgggc agttatttcg gcgtcgtgtt cgagtacctg ccggcgtttc tcgagcttgc
     9961 gccgtttctt gaccgacttg tagagaccgg ccgcggcttg ctggatggtg accgaatccg
    10021 gatgttcggc cggcagccgg tcgagctggc cgatcacccg gagggcggtc tccaggtcgt
    10081 cggggtcgat ccgttcgtcg cttttcggtc ggctgcgctg gtccatggtc aggaactgac
    10141 gaagggtatt agaggttgca acctctagtc tcggcccatg accaaacttc tcattgtgat
    10201 cgccagcgtc cgtcccggcc gggtcggcga atccgtcgcc aactggtttg tcccgatcgc
    10261 cgaagcggac gagcgcttcg aggtcgaggt ggccgacctg aaagagctcg atctcccctt
    10321 catggacgag ccgaacatgc cgggccggcg cgaatacaca aaggagcaca cgaagaagtg
    10381 gagcgcgatc gtcgaccggg ctgacgcctt cgtcttcgtc accccggaat acaacgcgac
    10441 cccggccccg gcgctcaaga acgcgattga ctacctctac cacgagtggt catacaagcc
    10501 ctgctccttt gtcagctacg gcggagtctg ggccggggcc cgcgcggtcc aggccctgaa
    10561 gccgacgctg acccagctgt cggtgatgcc ggtgccggag atggtgccga tccccggggt
    10621 ttccgggctg ctcgatgacc aggggaatct ccagggcacc gagatccacg aaaagagcgc
    10681 cggcggcatg ctgaattcgc tcgaaaagtg gtcgggcgcc ctgaagaccc tgcggcagta
    10741 acccgaaaaa ctcaagcccg aaaaactcaa ggtgccgcac gttcagcccc gtcacggggc
    10801 agaacgtgcg gcacctttgt cagctcaacc ggcggcgtag ctcgaagaca ccgcgccgcc
    10861 cggcttgccc aggatcgaga tcgatgcggt cttcttcttg gccccgttgg cgcttgtctc
    10921 gaccggcagt ttcaccttca tgcccgggct ggcctggcgg gtgacctgga tccggattga
    10981 aaccgaggcc gactttccgg cacgcaggtt acccggcgac ttgcaggccg gcatcgcctt
    11041 gatctgtttc ttcgccctcg ccggaacctt gacgcagacc ttgaacccgg tcgcgttcgt
    11101 ggtgctgggg ttcttcacgg ccacggtcat ggtccccttt ttgccgtact tgattttggt
    11161 cggcttgacg ctgatcttcc tgatcgtcag gctggccggc ggggccttgg ccacggggac
    11221 cgttgctttt gccgtgttgc cggcgccatc gaccacggtc accgaggcct tgccggcctt
    11281 cgggctcttg cagagcacct tgagtccgct gaccttcttg ccgtcctggc aggtccagtg
    11341 ggcggccacg atcttgccgg aaccgccgag ggcgccgccg aggaggctgg ctccctggcc
    11401 ggtccgtttg gcgacgctgg catcggccgt gagtggcggg tcaaccgtga tcgtgtcggt
    11461 ccagctccgg gtcaggccgt tgctgctcac cgttgccgtg acttcgtagc tgcccggccc
    11521 cgggaaggtg tggtcgaagc gctcgccgac gctggtggtg ccggtcgtgg tgtctccgaa
    11581 gtcccagctg atcgaggtcg ggaccggcgc ggtaccgtcc tgtgacctgg cggccgagcc
    11641 gaggatgttg aaggtcggat cggtcgactc gtactcgtcg gggtaccact ggatgcccgg
    11701 cttgctggcc acgctgcttt caaacgctgc gtggacggtc tccggcgtgg cggtgaaccc
    11761 gatctcattg agcccggcga gcgcgctgtt ggcgttggcc tggcccagct ccggtccgac
    11821 gttgtaatcc tcgaagtcgc tcgagccgaa ctgctggtcg gtgtagtcgg cgcgctggta
    11881 gtagtagccg agcatgtcgc cggcctgggc gatcacgttg acctggcggg ggccggtcac
    11941 accgtcgcgg atcgcgaaat tgacctcggg gaaggcttca ccggggttgg tcgcgaacgc
    12001 gacgtcaccg atccgggcgg cctcgacata ggtgccgatc acatcttctc cggagacctc
    12061 attcatgtag ggcggggcct tcgaacgcgg gatggtgcag cgttcgatcc cgtcgatgca
    12121 ggccccggga taggtgaaga tgctgatccc gagcagcagg tcgtcgttgt ctgcgggggt
    12181 ttccatgaac tgctcgctgg ccgcgatcgt gtcatcggtc agcggcgtgg acttggccat
    12241 ggtccgctgg atctcgttgg cgacgatctc gccctgggct tcgacctcgg aatacgcggt
    12301 gacgcttccc gggggttcct gacggcccag cgtgccggcc gccagcaccg cggtgccgcc
    12361 gttcagggca tcgagcttgc gcctggcgta gccgggccag tcggccgaca tctcgaagtt
    12421 gttgtcgtcc acctggtact ggtccgggtg gttcgggacg ttggcgtaga gggcattcgt
    12481 cgcgccggtg tcgggatcac gggcccagag gatcggcagg gcgttgtcgt actcgaaacc
    12541 gtcagggtga ttggtgccct gaccgttttg ccagatgaac gaacggatgt caccgacccc
    12601 ggtccagatc tcggacggcc tggcggcgtc ggcggcctcc gaagcggcgg tcaccgcggc
    12661 gtcgcttacc ttcttcaggt actcgccgtc gagggtgccc cagattccga ccacggtcgg
    12721 tgccgcgtga acgtgggtgc tggagacgat caccgattcg cggccgatgt cgtagccgcg
    12781 cgagttgagg gcgtcggcga tcttctcccg ggtggcgtcg attccgtagg gggcctgttc
    12841 accgtcgtag gcggcgaacc agccgaccag gtcaaccgaa acgaaggcga gcgcatcctt
    12901 gcccttgccg accacgaagg cgcgggcctc gaggttgtcg tgggtggtct gggtcgggcc
    12961 ctgcttgtag ccgtacccac cgacgtactg cggcgaatcc gggttgatgc tcgacgaagc
    13021 ggccccgacc tcgaatacgg ccggctcggc tgccgtggca cgactcgcgc cgaaagccag
    13081 gcagcagacg aatgcagcca aagcgaatgc gaacgcaaga tggcggcgcg tttctgcgaa
    13141 acgaatcccc ggaatcatca atttctcctc tcgccaggcg cctctagcgc cggactccct
    13201 attcccgaat cggcgcaggg gccgactcct cctgcggtga ggataccgca gggggtgagg
    13261 gcctatctca cgcgatcagg gtgaagatca attgccgccg gcaggaggcg gggccgtcgg
    13321 gtcgttgcct tcctcgccga agctctcgcg ggcgatgtac tcctcggcca gcagcaggtt
    13381 gtccttcgag cgctcgacca cgttgagcag gtcgcccatc agggccactt cctcgacctg
    13441 ctccttgacg aaccactgca tgaagtgctc ggcccggtag tccttgattt cgcgggcgag
    13501 ctcgaaaagt ccgaagatct cgctggaaac ccgctcctcc tgggccagcg cctccttgac
    13561 cggggtgatc ccgtcctcgt acttggtcgg ctgcgacttg acgtccggaa cctcgacctc
    13621 ttcgtcgacg tcgagcaggt actggatcat catcatcgcg tgctcacgct cttccagggc
    13681 ctggcggtag aagaacgcag ccaggcgcgg cagcgtttcg ctgtcgtagt agacggccat
    13741 cgccacatac tgctgggagg cggcgaattc attcgagatc tgttcgttca gcgccttcgc
    13801 gaaactcttc ttcttggcca tcttctgctc cgttcttcga ggggtcgatt caagtcgccg
    13861 acaccaacct agcgcagtcc gcggctttgg atcgccgtac gcgaggttag atgactctaa
    13921 ctctgatctg gctttccgaa gccgtttccg ggcaccttca tcgggctgac cggcagcccg
    13981 gcctcgggtt ccgccgcgtt gtagccgacc ggtgccgtgt cattcggatt gtccgggtga
    14041 aagagcgccg ccagatactg gatctggtcg cgaccgacgc ccagttccca ctggccgcca
    14101 ccgtaggcgc cgatcccttc ggcctcgcaa tggtcgtagg cgtcgagggt cgatttcaat
    14161 ccacccatcc gggagggctt gatgttgatc acggtgggct tgtgttcaag ctccgcgatg
    14221 tcggcgaccg aatggatcgg cgcgtcccag gtcacccggt gccagtcatc cttcagcacc
    14281 tcgcgggttt cgggggtcag gtccgggtcc tcgatccagg catccgggaa agccagaacc
    14341 agccggcggt aaagctccgg gtcggtttcg atgtcgacca ccgtgccctt gtactggccc
    14401 ttgaggtcga gcgtgttgac cgctccggtc tcgaccagct cgccgatcag ggcgtcatcc
    14461 cagtcgagtg acgggtcgag cttgaacttg aggtcggggt agcggtcgag cttgacccgc
    14521 agacggccga cgtccgactt cgtgccgccg ggaccgccgg ccagccccat cgaggcgacg
    14581 aaggtcagcg ggttcagcgg gcgatccagg tacccggcca gcgaggtgcc ggcctgccag
    14641 agagcgaggt cgagggcggc cgactcaaag gcccaacgac ggtagagccg ggagacctca
    14701 cgctcgggcg gggaggggaa gaggtcgagg ccgccgatca actcgcagaa ctcgccgacc
    14761 gtgttcggcc cggtcaggtc atgagtggcc ccggcatcct gaagggcgat ctggtcaagc
    14821 gcctcgtagg tgatgtcctc gcccagtccg gtctggccgg ccccttcgag gcggatcacg
    14881 gtcgtcaacc gctcgaactc cggcgaaggt ttgatcgata ggccctccat cgaccacgat
    14941 tcgatcttca acggcaactc cgcaacacgc tcgtaggtgc tcatggggtt gagcctactg
    15001 agggcgggta ggctccattc atgtcctccc tgatccatac ctgttaccgc gtctttgacc
    15061 tcgacgagtc gatcgacttc tacaagaagc tcggtctgct ggagcatcat cgcctgccga
    15121 tcggagacga ggcggtgaac gtctacatgg gccatgaagg cgacggggcc cgactcgagc
    15181 tgaccttcaa ctacgaccag caggagccct acgagatcgg caccggctac gggcacatcg
    15241 cgttcgtggt cgatgatctc gacagcaccc tggagggcct cgcagaccag ggcatcgcac
    15301 ccgaaaagcc tccctacacg gtcagcgagg gcgggtcgag gctctgcttc gtaaaagacc
    15361 ccgacggcta ccggatcgaa ctgatcgaag gcgacccggg tcgcgccgac taggaaatcc
    15421 ccgccagcca ggcggcttcg aggtcggcgt ccccggggaa ccggccgtcc agttcgagca
    15481 gcaccaggcc gtgcgccagg gcccagaagg cgcgggcccg tgcccggtcg ccgtcgactg
    15541 ccgcgagcag aggtgccgcg gcggcgtcct cgagtccggt cggcagattc tcgcggtcaa
    15601 gtggccgggt ggtggcgatc cggtagagcg acgggttggc gatggcccag gcccggtagg
    15661 aacggcccat ctcttccagg ccgtcggctg cggcgagtga ttcacgcatc tcgttgaggg
    15721 cctcggcggc cagcagcgtt tcaatctccc gacggctgct cacgtgcttg tagagcgagg
    15781 gagccttgat gccgagacgg tcggcgaggt tgcgcatggt cagggcctcc tcgccttcct
    15841 gctcgaggat cgaccgggcc gcggcggtga tctctgcgcg ccggctcaac tgccggccgt
    15901 ttctttctcc ggcgcaaggc cggccggcgc ttcgacgtcg cgttcggccc gcttcctgag
    15961 cgccgcgttc aaagcttcga atccccgctc ggtctggctg agaaccttgc ccatgaagcg
    16021 ggggaggagg ccggtgaatt cctcaccctg atcaagcctg gtcccaccgc cctccggggt
    16081 caggcgaaag tagtgctcgc catcgaagat gccccggaac ccgagccggc ccagccagcg
    16141 cagctcctgc ccgcgatcgg ccttgagcac cgtcggcctg aagttcatcg ccttgccccc
    16201 gggaggctgc atcctgagca caagcttttc gccttcccgg agttcgcccg aggcttcgat
    16261 gatgaacggg ttccagtccg ggtaggagga aaggtcggcc aggatctgcc agacgcggtc
    16321 gggcgaggca tcgatcaatt cggttgtgtg gatcgaaggc atgagcccga ggctaacaca
    16381 gttagccagc gcagacaaac actgttggcc gtatcgcctc gaagcaggcg cctcaggtat
    16441 cgagcaggcg gcgccgcagg gcgagcagag tgccatcttc gaggccgagt ccgtcccgca
    16501 tgtagccgtc gatcgacccg taacgctcct tcatcaagtc gaccgcggtc tccaggtacg
    16561 agcgatcgac cccgatcacc ggccggagca ggtcgaactc gatcccggct tcctcgatgt
    16621 ggctgaggat cggcttgagg gcgggcagca gctgctcgtt ggtcagcagg taatcgcggt
    16681 agacgtcctc ttcgtcgacc ccgaggaaga gcagggt
//
LOCUS       DAOQKN010000032        16562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_1594_length_16562_cov_5.396680, whole genome shotgun sequence.
ACCESSION   DAOQKN010000032 DAOQKN010000000
VERSION     DAOQKN010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 16562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16562
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1594_length_16562_cov_5.396680"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..2559)
                     /gene="pheT"
                     /locus_tag="PLJ64_03660"
     CDS             complement(43..2559)
                     /gene="pheT"
                     /locus_tag="PLJ64_03660"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNC92622.1"
                     /translation="MRVPYGWMADYCDPGLEPRDLAEKMAMTGTEVERVVTLGPPSAE
                     GFVIGKVLAVEKHPDADRLNVCQVDTGGETRTIVCGAPNVAAGQTVVVALPGAVMPGG
                     MEIKQAKLRGVESNGMICSERELELGEGHEGILVLGDGPAPGTPAGQLLPLAEPVLEL
                     EVTPNRTDCFGIYGVAREAHAISSAPLAPAPWDGGPIGPGLNSEPGDLDRLDDPVSDQ
                     KTADRVRILVQDEDLCPRFTARGYSEVKVGPSPTWLKARLIAAGMRPISNVVDITNYV
                     MWLTGQPMHAYDLDLVPEGELIVRRAENGEKLAALDGSEYELTSEMVAVADRGGLAGI
                     GGMMGGSVSEVSESTTTVLMESANWNGPNILKTSRDLGLRSEASSRFEKQLHPDLTLR
                     AQAVAARLMVEICGAVPMTGVVDVASGSPASAPIRLRAGRVERILGLPIEADRQAEAL
                     EMLGFGVEREGEDLLVTVPPDRYFDVTREIDLVEEVGRISDLDRNLPATLPSGAGRPG
                     GLSRQQALQRRAEDSLRESGFDEIVGWSFTDPGEKDRLRLAPEDPRAQAVKLANPLSE
                     DQSVMRTTLLGSVLGAARRNLSRGADRVALFESGRVYLPSGAFGPGPLGGDFPGNTRP
                     PSNEPQHLCALAVGSIDPSSWREPAAGSDFFALKGVLEHLAGGLGTAVTVERIPGAPT
                     QPFLHPGKSGVVAVGGQPIGWIGEIHPVVAAEYDLDSAVAFEIALADLLQSSPVGEEA
                     YEDFTPFPPVDRDLAVLVSDAETSAGVLATVQQAGGELLTGVSVFDVYSGEGVPEGEK
                     SLALRLRFRAPDRTLSDEEIEPLWSGIIAALESAGGKLRG"
     gene            complement(2556..3623)
                     /gene="pheS"
                     /locus_tag="PLJ64_03665"
     CDS             complement(2556..3623)
                     /gene="pheS"
                     /locus_tag="PLJ64_03665"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNC92623.1"
                     /translation="MVERIEEIEVEAQAAVEAADSAAELEEVRVRYLGRKAELTGMLR
                     GIADLPPEQRGPVGSTGNKVRKGLEGLIEAKVKALRNAELEQSLAGDRIDVTLPGSPA
                     RAVGHLHPITRTRRLIEDVMIGLGYQVMEGPEVEHDYYNFTALNHPEGHPARMLQDTF
                     YVDTDEHVEQVLLRTHTSPMQVRAMESAPPPLFMIVPGKTYRRDSDATHSPMFHQVEG
                     LAVGEGITLADLKGTLLAMLREIFGPDREIRMRPHFFPFTEPSVEFDVSCFRCGGSGS
                     LEDGSRCGLCKGIGWIELGGAGMVDPNVFGFVADSGYDPDSVTGFAFGFGIERIAMLQ
                     HNVGDLRLFFDNDLRMLEQFS"
     gene            complement(3652..4374)
                     /locus_tag="PLJ64_03670"
     CDS             complement(3652..4374)
                     /locus_tag="PLJ64_03670"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNC92624.1"
                     /translation="MIVSKENEKLKLVRKLGQKKHRRKLGMFVTEGEDLARAGLAAGH
                     EPVAMLVHPETDLPGEKVEPDLLDGVAALASGTRVIGIWSEIWAEPELLTADKCIFLD
                     GVADPGNVGTIIRTADALVGGTVVLGPGSADPFSGIAVRSSMGSIFTQPVVKAEIGQI
                     PAPRIATVPQGGERPGPVEGPVTLCLGSEREGLSPEILKECGITWTIPQRSGGAESLN
                     VAAAAAIACERISSQPGESAQD"
     gene            complement(4468..4827)
                     /gene="rplT"
                     /locus_tag="PLJ64_03675"
     CDS             complement(4468..4827)
                     /gene="rplT"
                     /locus_tag="PLJ64_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNC92625.1"
                     /translation="MARVKRSVNARKKRRKVLEEAKGYYGRKNSSYKLAKEQVMRSGA
                     YAYRDRRVRKREFRRLWITRINAAARLEGMSYSELMHGLSAAGVEVNRKMLADIAVND
                     REDFRRFVEIAREGAAA"
     gene            complement(4828..5025)
                     /gene="rpmI"
                     /locus_tag="PLJ64_03680"
     CDS             complement(4828..5025)
                     /gene="rpmI"
                     /locus_tag="PLJ64_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNC92626.1"
                     /translation="MPKMKTHSGAKKRFKRSAKGKLRGRHAYSSHILEKKSPKRKRNM
                     GKPAHIAEVDAPRVNKLLGGK"
     gene            5154..6233
                     /gene="wecB"
                     /locus_tag="PLJ64_03685"
     CDS             5154..6233
                     /gene="wecB"
                     /locus_tag="PLJ64_03685"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNC92627.1"
                     /translation="MRIVHVLGTRPNFVKMAPLIAAISRRSPETAQLIVHTGQHYDHL
                     MSEIFFDQLGVPAPDFMLEVGSGSHALQTARVMERLEPLLIENRPDLVVVPGDVNSTV
                     AAALTAVKLEIPVAHLEAGLRSYDRSMPEEINRIITDQIADTLWVHSEEALRNLELEG
                     IPAGRAGFVGNTMIDSLVALEDRFRPLETCRSFGLEPGSYLLVTLHRPALVDGPLLFD
                     AIAALNQVSAVMPVLFPVHPRTRARLAGNAELAENLHLVDPAGYLEFLSLEADAGAVL
                     TDSGGIQEETTYLGIPCFTLRDNTERPVTIELGTNVLLGLDPARITEIPAIIAGADPS
                     AAPPEPPPLWDGHAADRIAEQLAAT"
     gene            complement(6498..7334)
                     /gene="infC"
                     /locus_tag="PLJ64_03690"
     CDS             complement(6498..7334)
                     /gene="infC"
                     /locus_tag="PLJ64_03690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNC92628.1"
                     /translation="MPIPRRFDRRPPDRDTTRINERIRVPKVRLIGADGEQVGIVDTD
                     QARQMAREAELDLVEVAANAKPPVCRLLDYSKYKYEQEQKQKAARKHQTQVNIREIKL
                     RPKIADNDYNTKKGHVERFLKHGDKVKVTIMFRGREQAHPERGRDLLYRLFEDLGEEL
                     AVIESRPLQEGRNMSMLLGPSKEATREAEARKQARKKAKGKNRGGEAPEVPDHPNAVA
                     PDAVPDHPNAVAPDAIPDHPNAVAPDAEAPEPTEPEDPPAGPEAADEAAAPAEEPAET
                     SD"
     gene            complement(7428..9350)
                     /gene="thrS"
                     /locus_tag="PLJ64_03695"
     CDS             complement(7428..9350)
                     /gene="thrS"
                     /locus_tag="PLJ64_03695"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNC92629.1"
                     /translation="MPEAAANITVSLPDGTPLELDPGATGADAALAIGEGLARAALAL
                     KVGDEVRDLSAPVSDGEEISIITSRDPEGLELIRHDAAHVMATAVQELYPGTKVTIGP
                     AIENGFYYDFEFPEGVKITDAELPAIEEAMRRHIKADETFERRDVPVAEAIEIFKAQD
                     ERFKVELIEDLIENEGAETVSLYRNGPFEDLCRGPHGPGTKRIKVIKLNSVAGSYWRG
                     DENREQLTRIYGTAFFSKDDLAEYLERIEQAKARDHRRLGPELDLFMLRDEAPGMPFW
                     LPNGTVLLGLIEQEVRQQLARRGYQEIATPQVMDESLWHRSGHWDNYKDDMYFMEVDE
                     RRYALRPMNCPGACLVFGSTRHSYRDLPLRLAEFGRVTRNEREGVLHGLLRVRAFTQD
                     DAHVYCTIDQVETEVSEICEAIDELYARFGFENVEVELSTRPEKSLGSDELWEKAEST
                     LKSALDSQNREYRINPGDGAFYGPKIDFHVTDALGRSWQLGTCQLDFQMPERFELHYT
                     DSEDGQERPVMIHRALLGSMERFAGILIEHYAGRFPAWLAPVQARIVPVSDKHLDYAR
                     QVAEDLKAEGARVSVEERSESVGKKIRAAELGRFPYMLVVGDREMEAGEVSVRSHEDG
                     ELGSMAVEKFCMLLDS"
     gene            complement(9384..10424)
                     /locus_tag="PLJ64_03700"
     CDS             complement(9384..10424)
                     /locus_tag="PLJ64_03700"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC92630.1"
                     /translation="MKSQGVMGVVQKLVGLAIGLLFAMPDSLMRKIFGDPPAAVAALR
                     PDAWAMGRLIGLAEDKRSEWNPEEAREETEFLAGAVSRPVTGVITQDWLLEAGDHSLP
                     ARLFTPGANPLAGPMLVHFHGGGWVQGSINSHHGACAWLAREAGVRVLSVDYRLAPEH
                     PFPAQADDALAAWRSVMSDPARFGADPEKVGVIGDSAGAQMCAVLCLDLKAAGEPQPA
                     CQVLIYPVTDCTGSFPSRESFARGFYLTRARMDWFEDCFVPEGEAENPRVSPLFADDL
                     SGLAPALVSVSLADPLRDEGVAFAGRLIEAGVPVTLDTMPMLHAWFNLTSSRSSRQGH
                     RILAGRITDLLG"
     gene            10488..11900
                     /locus_tag="PLJ64_03705"
     CDS             10488..11900
                     /locus_tag="PLJ64_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92631.1"
                     /translation="MTGRKCALWLAAAISVLTFATAATANGATRERPCNGAVELCSKT
                     LDQVVLPGTHNSMSNEEYGWILPNQHYAIPTQLEYGVRAFLIDTHYGKPSAGSGPSNV
                     ADWNNGTDGSPKGVAGTYLCHQNCSWGASDLTAELGKVTDFLASHPREVLIFMVEDGI
                     YPQDFATAVNDSALSDYIYTGPTDSYPTLGEMIASNQRVVVFSENHTGNDSDYAEVPW
                     YHRGYNGPMQETPYNFRKDESNVDLTTQQAMAQLTDSANLNSSCRPNRGGTTGQLFLM
                     NHWVNGLIDAGTTGNTFTPDPAVAQVLNQPDVLVARAQACEARRGKLPNIVAVDDFGD
                     GDLTDAVRQLNGVTAKPFFEVAKPKGVTVKSGARASFRLQIRNWGSAESALTRVCASV
                     PGKLAIKPKCQTVFVTQANPGKGTATVKVSTRRIYQGRFKGSKSGPVRFTITGGGDSV
                     TTSSTLKVNPLKPKKPKRRN"
     gene            complement(11952..12024)
                     /locus_tag="PLJ64_03710"
     tRNA            complement(11952..12024)
                     /locus_tag="PLJ64_03710"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11989..11991),aa:Val,seq:gac)
     gene            complement(12085..12843)
                     /locus_tag="PLJ64_03715"
     CDS             complement(12085..12843)
                     /locus_tag="PLJ64_03715"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNC92632.1"
                     /translation="MPIPEKIQGFIDRQADEAPVKYDDLRAVVFNGTLKRSPEPSQTE
                     ALLAIPIGILERVGVRVDLVRTVDHVIPPGVWPDMTGHGYEEDDFPAIYRELVEPADI
                     IILAGPIWLGDQASPTRAIIERLYAYSGEVNDAGQWSYYGKVGGAVTTGNEDGGKHVS
                     AQLLYALQHIGLTIPPQTDSYWNGEAGPGDSYADPGSGGPENAWTTRNATFMTWNILH
                     FARMLKDAGGVPAYGNSTRDWDLTQPDHPNPEYR"
     gene            13087..15426
                     /locus_tag="PLJ64_03720"
                     /pseudo
     CDS             13087..15426
                     /locus_tag="PLJ64_03720"
                     /inference="COORDINATES: protein motif:HMM:NF040071.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6351 family protein"
     gene            15477..15550
                     /locus_tag="PLJ64_03725"
     tRNA            15477..15550
                     /locus_tag="PLJ64_03725"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15511..15513,aa:Gly,seq:gcc)
     gene            15583..15653
                     /locus_tag="PLJ64_03730"
     tRNA            15583..15653
                     /locus_tag="PLJ64_03730"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15615..15617,aa:Cys,seq:gca)
     gene            complement(15740..16186)
                     /locus_tag="PLJ64_03735"
     CDS             complement(15740..16186)
                     /locus_tag="PLJ64_03735"
                     /inference="COORDINATES: protein motif:HMM:NF018257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfocyanin-like copper-binding protein"
                     /protein_id="HNC92633.1"
                     /translation="MAAATLVLALGISACGSSSDDSGESSDTGTTTSTTQAPANAQSV
                     KISLTEMAISASPDSVKAGDVAFDVTNDGNIEHELVVVKTNKKAGDLPTDASGKADET
                     GSVGETEVQAGESSELDLKLAAGHYALICNLPGHYKAGMYTDFTVK"
     gene            complement(16269..>16562)
                     /locus_tag="PLJ64_03740"
     CDS             complement(16269..>16562)
                     /locus_tag="PLJ64_03740"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HNC92634.1"
                     /translation="ETIGEATNNVAEYRALIKGIGLARELGATELQIHGDSELVVKQM
                     LGQYKVKHPDMKPLHAEAKAALEGLDSWSISHVRREQNAEADALVNQALDSQA"
BASE COUNT         2799 a         5609 c         5272 g         2882 t
ORIGIN      
        1 acccgggctt tcgccccttg cggggagatc ggctgtgcgg cctcagccac gcagcttccc
       61 tccggccgat tcgagcgcgg cgatgatgcc cgaccagagc ggctcgatct cctcgtcgct
      121 gagggtccgg tccggcgccc ggaagcgcag cctcagggcg aggctcttct caccctcggg
      181 aacgccttcg ccgctgtaga catcgaaaac ggagaccccg gtgaggagct ccccgccggc
      241 ctgctgaacc gtggccagga cgccggcgga agtttcggcg tcagaaacca gcaccgccag
      301 gtcacggtcg accggcggga agggtgtgaa gtcctcatat gcctcctcgc cgaccgggct
      361 tgactgaagc aggtcggcaa gggcgatctc gaaggcgacc gccgagtcaa ggtcgtattc
      421 ggcagcgacc acggggtgga tctcgccgat ccagccaatc ggctgacccc cgaccgcgac
      481 cactcccgat ttgcccggat ggagaaaggg ctgcgtcgga gcgcccggga tccgctccac
      541 ggtcaccgcg gtgccaagcc ctccggccag gtgttcaagc acgcccttga gagcgaagaa
      601 atcggatccc gcggccggtt cgcgccagga cgaaggatcg atcgaaccaa ctgccagcgc
      661 acacaaatgc tgtggctcgt tcgaaggggg cctggtgttg ccggggaaat cgcccccgag
      721 cgggcccggt ccgaacgctc cggagggcag atagacccgc ccggattcaa agagcgcgac
      781 ccggtcggct ccccgcgaaa ggttccgccg ggcagcgccg agcaccgagc cgagcagcgt
      841 ggtccgcatc accgactggt cctcggagag cggattggcc agcttcacgg cctgggcccg
      901 cggatcctcc ggggccagcc tgagccgatc cttctcgccg gggtcggtga agctccagcc
      961 gacgatctcg tcgaagcccg actcgcgcag ggaatcctcg gcccgccgct gcagcgcctg
     1021 ctggcggctg agcccgccgg gcctgccggc accggatggc agggtggcgg gcaggttgcg
     1081 gtcgaggtcg ctgatccggc cgacctcttc gaccaggtcg atttcccggg tcacgtcgaa
     1141 gtaccggtcg ggcggcaccg tgaccagcag gtcttcgcct tcccgctcga ccccgaagcc
     1201 cagcatttca agcgcctcgg cctggcggtc ggcttcgatt ggcagtccga gaattctctc
     1261 cacccggccg gcccggagcc ggatcggcgc ggaagcgggg ctcccggaag cgacgtcaac
     1321 cacgccggtc atcggcaccg caccgcagat ctcgaccatc agccgggcgg ccacggcctg
     1381 ggcccgcagg gtcaggtcgg gatgaagctg cttttcgaag cgcgaggaag cctccgaacg
     1441 gagcccgagg tcgcgcgagg tcttgaggat gttcgggccg ttccagttgg ccgactccat
     1501 cagcaccgtg gtcgtggatt ccgacacctc ggaaaccgag ccgcccatca tgccgccgat
     1561 tccggccagg ccgccccggt cggcgacggc gaccatctcc gaggtcagtt cgtactcgct
     1621 gccgtccagc gcggccagct tctcgccgtt ctcggcccgc cggacgatca gctcgccctc
     1681 cgggaccagg tcgaggtcgt aggcatgcat cggctggccg gtcagccaca tcacgtagtt
     1741 ggtgatgtcg accacattcg agatcggccg catgcccgca gcgatcagcc tcgccttgag
     1801 ccaggtcggt gaggggccaa ccttcacctc gctgtatccg cgagcggtga agcgcgggca
     1861 gaggtcctcg tcctgaacca ggatccggac ccggtcggcg gtcttctgat cgctgacggg
     1921 atcatccaga cggtccaggt cgccgggctc ggaattcagt cccgggccga tcgggccgcc
     1981 atcccagggc gctggcgcca gcggcgccga gctgatcgcg tgggcttcac gggcgacccc
     2041 gtagatgccg aagcagtcgg tccggttcgg ggtgacttca agttccagca ccggctcggc
     2101 cagcggcagg agctggccgg ccggggtgcc cggggccggc ccgtcaccga gcaccaggat
     2161 gccttcatgg ccctcgccga gttccagctc ccgttccgag cagatcattc cgttcgattc
     2221 gaccccccgg agtttcgcct gcttgatctc catgccgccg ggcatcaccg ccccgggcag
     2281 ggcgaccacc acggtctggc cggcggccac gttcggggcg ccgcagacga tcgtcctggt
     2341 ctcgccgccg gtgtccacct gacagacgtt cagccggtcg gcatccgggt gcttttcgac
     2401 cgcgagcacc ttgccgatca cgaagccctc ggccgaaggc ggccccagag tgaccaccct
     2461 ttcgacctcg gttccggtca tcgccatctt ttcggcgagg tcccgcggtt cgagcccggg
     2521 gtcgcagtag tcggccatcc atccgtaagg gactctcatg agaactgctc cagcattcgc
     2581 aggtcgttgt caaagaagag ccgcaggtca ccgacgttgt gctgcagcat cgcgattcgc
     2641 tcaattccga aaccgaaggc gaatccggtc accgagtcgg ggtcgtagcc ggagtcggcc
     2701 acgaatccga acacgttcgg atcaaccatc ccggccccgc cgagttcaat ccagccgatc
     2761 cccttgcaga gaccgcagcg ggaaccgtct tcgagcgacc ccgagccgcc acagcggaag
     2821 caggaaacgt cgaattccac cgagggttcg gtgaagggga agaagtgcgg gcgcatgcgg
     2881 atttcccggt cgggaccgaa gatttcgcgg agcatcgcca gcagggttcc cttgaggtcg
     2941 gccagggtga tcccctcgcc gaccgccagg ccctcgacct ggtggaacat cgggctgtgg
     3001 gtggcatcgg agtcgcgccg gtaggtcttg cccggcacga tcatgaagag cggcggcggc
     3061 gccgactcca tggcccggac ctgcatgggt gaggtgtggg tccgcagcag aacctgctcg
     3121 acgtgctcgt cggtgtcgac gtagaaggtg tcctggagca tccgggcagg atggccttcg
     3181 ggatggttga gcgcggtgaa gttgtagtag tcgtgctcga cttccgggcc ttccatcacc
     3241 tggtagccga ggccaatcat cacatcctcg atcagccgcc gggtccgggt gatcggatgc
     3301 aggtggccga ccgctcgggc cggactgccc ggcagggtca cgtcgatccg gtccccggcc
     3361 agggactgct ccagttcggc gttcctgaga gctttgacct tcgcttcgat caggccctcg
     3421 aggcccttcc ggaccttgtt gccggtgctg ccgaccgggc cgcgctgctc cggtggcagg
     3481 tcggcgatac cccgcagcat gccggtcagc tcggccttgc ggcccaggta acggacccgg
     3541 acctcttcga gttcggccgc cgagtctgcg gcttcgaccg ccgcctgtgc ctcgacttcg
     3601 atttcctcta tccgttcaac catttcttcc ccggttcagc ttcctttttc ctcagtcttg
     3661 tgcggattcg cctggttgcg aagatatccg ctcgcaggcg atcgccgccg cggccgcaac
     3721 gtttagcgat tcggctccgc cggaccgctg cgggatcgtc caggtgatcc cgcactcctt
     3781 caggatttcc ggcgaaagcc cttcgcgctc ggacccaagg cagagagtca ccggaccttc
     3841 gaccggtccc ggccgctccc ctccctgcgg gaccgtggcg atccgcgggg ccgggatctg
     3901 cccgatctcc gccttcacca ccggctgggt gaagatcgaa cccatgctgg agcgcaccgc
     3961 gatcccggag aaggggtcgg cgctgcccgg cccgagcacc accgtgccgc cgaccagggc
     4021 gtcggcggtc cggatgatcg tgccgacgtt gcccgggtcc gcgactccgt cgaggaagat
     4081 gcatttgtcg gcagtcagca gctccggttc ggcccagatc tcggaccaga tcccgatcac
     4141 ccgggtgccg gaggcgagtg cggcaacccc gtccagcagg tcaggttcga ccttctcccc
     4201 cggcaggtcg gtctcgggat gcaccagcat cgccaccggc tcatggcctg cggccagacc
     4261 cgcccgggcc aggtcttcac cctcggtgac gaacattccc agcttccggc ggtgcttctt
     4321 ctggcccagc ttgcggacca gtttcagttt ttcgttttcc ttgctgacga tcatggttcg
     4381 gtctgttctc tttccggccc ccgaaaacaa aaagggcggc tcgcagatcg cgaagccgcc
     4441 cggtgggttc gaggtgccgt cagcgcacta ggcggcggca ccctcccggg cgatctcgac
     4501 aaatcggcga aagtcctcac ggtcgttgac cgcgatgtcg gcgagcatct tgcggttgac
     4561 ctcgacgccg gcggcgctga ggccatgcat cagctcggag tagctcatgc cctcgaggcg
     4621 ggcagctgcg ttgatgcggg tgatccagag gcgacggaat tcccgcttgc ggacccggcg
     4681 gtcacggtag gcgtaggcgc ccgaccgcat gacctgttcc ttggccagct tgtacgagga
     4741 gttcttgcgg ccgtagtagc ccttggcctc ttcaagtacc ttgcgccgct tcttgcgggc
     4801 gtttaccgaa cgcttcacgc gagccattta tttgcccccc agaagcttgt tgacacgcgg
     4861 ggcatccact tcggcgatgt gggcaggctt gcccatgttc cgcttgcgct tcggcgactt
     4921 cttctccaga atgtggctgg agtacgcatg ccgcccgcgc agcttgcctt tggccgagcg
     4981 cttgaagcgc ttcttggccc cggaatgtgt cttcatcttc ggcatgagcc ggaacattca
     5041 atcaaaattt ccggcctgtc gcctttcccc cccggatacg ggatgaatcc cagcgccaat
     5101 cggctctgac tcgcaccgcc cccggccgcc ggcgccgata ctctggcccc ctgatgcgga
     5161 tcgtccacgt gctcggaacc cggcccaact tcgtcaagat ggcgccgctg attgccgcga
     5221 tttcccggcg ctcgccggag accgcgcaac tgatcgtcca cactggccag cattacgacc
     5281 acctgatgag cgagatcttc ttcgatcagc tcggagtccc ggctccggac ttcatgctcg
     5341 aggtcggctc cggcagccac gcgctgcaga ccgcgcgggt gatggaacgg ctggaacccc
     5401 tgctgatcga gaaccggccc gacctcgtcg tggtccccgg ggacgtcaat tcgaccgtgg
     5461 ccgcagcgct caccgcggtg aaactggaga tcccggtcgc ccacctcgag gccggccttc
     5521 gcagctatga ccgcagcatg ccggaggaga tcaaccggat catcacagac cagatcgccg
     5581 acaccctctg ggtccattcg gaggaggcgc tgcgcaacct ggagctggaa ggcatcccgg
     5641 ccgggcgggc cggtttcgtc ggcaacacga tgatcgacag cctggtcgca ctcgaggacc
     5701 gcttccgccc gctggaaacc tgccggtcct tcggtctcga accaggctcc tacctgctgg
     5761 tcaccctcca ccgccccgcc ctcgtggacg ggccgctcct ctttgatgcg atcgccgccc
     5821 tcaaccaggt ctccgcggtg atgccagtgc tcttcccggt ccacccccgc acccgggccc
     5881 ggctggcggg caacgccgaa ctggcggaga acctccacct ggtcgacccc gccggttacc
     5941 tcgagttcct ctcgctggaa gccgacgccg gtgccgtact gaccgattcc ggcggaatcc
     6001 aggaggagac gacctacctc ggcatcccct gcttcaccct gcgcgacaac accgagcgcc
     6061 cggtcacgat cgagctcggc accaacgtcc tgctcggcct cgaccccgcc cggatcaccg
     6121 agatcccggc aatcatcgcc ggagcggatc cgtcggctgc gcctcctgaa ccgccgcctt
     6181 tgtgggacgg ccacgcggcc gaccgcatcg cggaacaact ggcggctacc tgaaacttcg
     6241 gcagtcacca atccccctag acgggacttc gcgtagatca ggcagcgact gctgtctgac
     6301 gaaccgagtc cagcacttga atcagctcat ccaagtcggc agccccccag ggtgaccaac
     6361 cggcaaacga gaaggcgatt ctggccacat agcgctcctt ttggccatct cgattccgaa
     6421 ttgcgatgaa ctgtcggcta ctcggccaga gacgcggcca cgtcagccgc ttcagactcg
     6481 attggcttcg aactgaatca gtccgaggtt tcggccggtt cttcggccgg ggccgctgct
     6541 tcgtcagcgg cttcgggccc ggccggcgga tcttccggct cggtcggctc gggagcttcg
     6601 gcgtccgggg cgaccgcgtt cgggtggtcc gggatcgcgt ccggggcgac cgcgttgggg
     6661 tgatccggaa ccgcgtccgg agccaccgcg ttcgggtggt cgggaacctc gggggcttcc
     6721 ccgccgcggt tcttgccctt ggccttcttc ctggcctgct tgcgcgcctc ggcctcgcgg
     6781 gtcgcttcct tggaggggcc gagcagcatg ctcatgttgc gcccttcctg gagcggcctc
     6841 gattcgatca cggcgagttc ttcgccgaga tcctcgaaaa gtctgtaaag caggtcgcgg
     6901 ccccgttccg gatgggcctg ttcgcggccg cggaacatga tcgtgacctt gaccttgtcc
     6961 ccgtgcttga ggaaacgctc gacgtgaccc ttcttcgtgt tgtagtcgtt gtcggcgatc
     7021 ttcggacgca gcttgatctc gcggatgttg acctgggtct ggtgcttgcg ggcggccttc
     7081 tgcttctgct cctgctcgta cttgtacttc gagtaatcga gcagccggca gaccggcggc
     7141 ttcgcatttg ccgcgacctc gaccagatcg agttccgcct cgcgggccat ctgcctcgcc
     7201 tggtcggtat cgacgattcc cacctgctcg ccgtcggcgc cgatcagccg caccttgggc
     7261 acgcggatgc gctcgttgat acgagtcgtg tcccgatcgg ggggacgccg gtcaaacctg
     7321 cgcggaattg gcactagcta agaaccaagg ggcaggtcaa gcggacaaga aacgggtagg
     7381 cgagcacgcg ggcgcagcgg ggcgcgagtt ctttttcgtg cggcgaatca actgtcaagc
     7441 agcatacaga acttttcgac agccatcgag ccgagctccc cgtcctcatg cgagcgaacc
     7501 gagacctcgc cggcctccat ctcccggtca ccgaccacca gcatgtacgg gaacctgccc
     7561 agctcggccg cgcgaatctt cttgccgacc gattcggagc gctcctcgac cgagaccctg
     7621 gctccctccg ctttgaggtc ctcggcgacc tggcgggcgt agtcgaggtg cttgtcggag
     7681 accgggacga tccgggcctg gaccggggcc agccaggccg ggaaccggcc cgcgtagtgc
     7741 tcgatcagga tcccggcgaa acgctccatc gaaccgagca gcgcgcggtg gatcatcacc
     7801 ggccgctcct gcccgtcttc ggagtcggtg tagtgaagct cgaagcgctc cggcatctgg
     7861 aagtcgagct ggcaggtgcc gagctgccag gagcggccca gggcatcggt cacgtggaag
     7921 tcgatcttcg gcccgtagaa ggcgccgtcg ccggggttga tccggtactc gcggttctgg
     7981 ctgtcaagcg cgctcttcag ggtcgattcc gctttctccc agagctcgtc gctgccgagc
     8041 gacttctcgg gacgggtcga taactccacc tcgacgttct cgaacccgaa gcgggcgtag
     8101 agctcgtcga tcgcctcgca gatttccgac acctcggtct cgacctggtc gatcgtgcag
     8161 tacacgtgcg cgtcatcctg ggtgaaggcg cggaccctga gcaggccgtg gagcactccc
     8221 tcccgctcgt tccgggtcac ccggccgaac tcggccagcc gcagcggcag gtcacggtag
     8281 gaatgccggg tcgaaccgaa gacgaggcag gcgcccgggc agttcatcgg ccgcagcgcg
     8341 taacgacgct cgtcgacctc catgaagtac atgtcgtcct tgtagttgtc ccagtggccg
     8401 gagcggtgcc agagcgactc gtccatcact tgcggggtgg cgatttcctg atagccgcgg
     8461 cgggccagct gctggcggac ttcctgctcg atcagcccga gcaggacggt gccgttcggc
     8521 agccagaagg gcatgcccgg tgcctcgtct cgcagcatga aaaggtcgag ttcggggccg
     8581 agccggcgat ggtcgcgggc cttggcctgc tcgatccgct caaggtactc ggcgaggtcg
     8641 tccttgctga agaaggcggt gccgtagatc ctggtcagtt gctcgcggtt ctcgtcgccg
     8701 cgccagtagg aaccggcgac cgagttcagc ttgatcacct tgatccgctt ggttccggga
     8761 ccgtggggcc cgcggcagag atcctcgaag ggaccgttgc ggtaaagcga aacggtctcg
     8821 gcgccttcgt tttcgatcag gtcctcgatc agttcgacct tgaacctctc atcctgggcc
     8881 ttgaagatct cgatcgcctc ggcgaccggc acgtcgcgac gctcgaaggt ttcgtcggcc
     8941 ttgatgtggc ggcgcatcgc ttcttcgatc gccggcagtt cggcgtcggt gatcttcacg
     9001 ccctcgggga actcgaagtc gtagtagaag ccgttctcga tcgccggacc gatcgtcacc
     9061 ttggtgcccg ggtagagctc ctggaccgcg gtcgccatca cgtgcgcggc atcgtggcgg
     9121 atcaactcga gaccctcggg gtcacggctg gtgatgatcg agatctcctc gccgtcactg
     9181 accggggcgc tgagatcacg aacctcgtcc ccgaccttga gtgccagcgc ggcccgggcc
     9241 aggccttcgc cgatcgccag ggccgcgtcg gcaccggtcg cgcccgggtc gagctcgagc
     9301 ggggtgccgt caggcaggga tacggtgatg tttgcggcgg cttccggcat cggtcaatcg
     9361 tattgaaggc ggggacctcg ggatcagccg agcagatcgg tgatccgccc ggccagaatc
     9421 cggtggccct ggcgggagga acggctggag gtgaggttga accaggcgtg aagcatcggc
     9481 atcgtgtcga gggtgaccgg gaccccggcc tcgatcaacc tcccggcgaa ggcgactcct
     9541 tcatcccgca gcggatcggc cagcgacacg gaaaccagcg ccggggcaag accggaaagg
     9601 tcatccgcaa agagtggcga gacgcgcggg ttttccgctt ctccttcggg cacgaagcag
     9661 tcctcgaacc agtccatccg ggccctggtc aggtagaagc ccctggcaaa cgattcccgg
     9721 gaggggaacg acccggtgca gtcggtgacc gggtagatca ggacctggca ggcgggctgc
     9781 ggctcccccg ccgctttcag gtcgaggcag agcaccgcgc acatctgggc accggcgctg
     9841 tcgccgatca cccccacctt ctccgggtcg gccccgaagc gggccgggtc gctcatcacg
     9901 gatcgccagg cggccagcgc gtcatcggcc tgggccggga aagggtgctc cggcgccagc
     9961 ctgtaatcaa ccgaaagaac ccggaccccg gcttcgcggg caagccaggc acaggctccg
    10021 tgatgggagt tgatcgagcc ctggacccaa ccgcccccgt gaaagtgaac cagcatcggc
    10081 ccggccagcg gattggcccc gggagtaaag aggcgggcgg gcagtgagtg atccccggcc
    10141 tccagcagcc aatcctgggt gatcacgccg gtgaccggac ggctgaccgc gccggccagg
    10201 aactcggttt cctctcgcgc ttcttcggga ttccattcgc tgcgcttgtc ctcggccagg
    10261 ccgatcagcc ttcccatcgc ccaggcgtca ggcctcagcg cggcgaccgc ggccggcggg
    10321 tcaccgaaga ttttccgcat cagtgaatcc ggcatcgcga agagcaggcc gatcgcgagt
    10381 ccgaccagct tctgaacgac ccccatcacc ccttgagact tcacggagcg catcgtattg
    10441 gccgcaactg cgacttcact tcgcggttag ttgagatgat gaattcaatg accgggagaa
    10501 agtgtgcgct gtggctcgcc gcagcgatca gtgtcctgac cttcgctacc gccgccacgg
    10561 cgaacggcgc aacgcgagag cgaccgtgca acggcgcggt cgagctctgc tcgaagacgc
    10621 tcgaccaggt ggtcctgccg ggcacccaca actcgatgtc aaacgaggaa tacggctgga
    10681 tcctgcccaa ccagcattac gcgattccga cccagctcga gtacggcgtg cgggcgttcc
    10741 tgatcgacac gcattacggc aagccttccg caggatctgg cccctcgaat gtcgcggatt
    10801 ggaacaacgg gaccgacggt agcccgaagg gcgtcgccgg cacctacctc tgccaccaga
    10861 actgcagctg gggagcgagc gacctgactg ccgagctcgg caaggtcacc gactttctgg
    10921 cttcgcatcc ccgcgaagta ctgatcttca tggtcgagga cgggatctac ccccaggact
    10981 tcgccaccgc ggtgaatgac agcgccctga gtgactacat ctacaccggt ccaaccgact
    11041 cgtaccccac gctgggggag atgatcgcga gcaaccagag ggtggtcgtg ttttcggaaa
    11101 accacacggg caatgattcc gactatgcgg aggttccctg gtaccaccgc ggttacaacg
    11161 gaccgatgca ggaaaccccg tacaactttc gcaaggacga atccaacgtc gacctgacta
    11221 ctcagcaggc gatggcgcag ctgaccgatt cggccaacct gaattcgagt tgccggccga
    11281 accggggcgg caccaccggg cagctcttcc tgatgaacca ctgggtcaac ggactgatcg
    11341 acgccggcac gaccggcaat accttcactc ccgaccccgc ggtcgcacag gtcctcaacc
    11401 agcccgatgt gctcgtcgcc cgggcgcagg catgtgaggc gcgtcggggc aagctgccga
    11461 acatcgttgc cgttgatgac ttcggtgatg gtgacctgac cgacgcggtc cggcagctga
    11521 acggcgtgac cgcgaagccg ttcttcgagg tcgcaaagcc aaagggcgtc accgtcaagt
    11581 ccggcgccag ggcgagcttc aggctccaga tcaggaactg gggcagcgcg gaatcggcgt
    11641 tgaccagggt ctgcgcgagc gtccccggca agctggcgat caagccgaaa tgccagacgg
    11701 tcttcgtgac ccaggccaac cccggcaagg gaacggcaac cgtgaaggtc agcacccgca
    11761 ggatctacca gggccggttc aagggcagca agtccggccc ggtcaggttc acgatcaccg
    11821 gcggtggtga cagcgtcacg acctcgtcca ccctcaaggt gaacccgctc aagccgaaga
    11881 agccgaagcg ccggaactga gccgggctcc cgctgaaaca cgaaacccgg ctggagggcc
    11941 gggtttccga gtgggcacaa ctgggctcga accagtgacc tccaccgtgt cgaggtggcg
    12001 ctctaccaac tgagctatgc gcccggggac ggagagccta gcgcagggcc gccccggtcc
    12061 gcaccgccgc accggcaact cccctcatcg gtattccggg ttgggatggt cgggctgcgt
    12121 gaggtcccag tccctggtcg agttgccgta ggcgggcacg ccaccggcgt ccttcagcat
    12181 gcgcgcgaaa tggaggatgt tccaggtcat gaaggtggcg ttgcgggtgg tccaggcgtt
    12241 ctccgggcca ccgctcccgg gatcggcgta ggaatcaccg gggccggctt caccgttcca
    12301 gtacgagtcg gtctgcggcg ggatcgtcag cccgatgtgc tgcagcgcgt agagcagctg
    12361 ggcgctcaca tgtttgccgc cgtcctcgtt gccggtcgtg accgctcccc cgaccttgcc
    12421 gtaataggac cactgaccgg catcgtttac ctcgccggaa taggcgtaga gccgttcgat
    12481 gatcgcccgg gtcggggagg cctggtcacc cagccagatc ggaccggcga ggatgatgat
    12541 gtcagccggc tcgaccagtt cacggtagat cgccggaaag tcatcttctt cgtaaccgtg
    12601 cccggtcatg tccggccaga ctccgggcgg gatcacgtga tccacggtgc ggacgaggtc
    12661 gaccctgacg ccgacccgtt cgaggatccc gattggaata gcgagcagcg cctcggtctg
    12721 gctcggctcc ggagatcgct tgagagtgcc attgaaaacc accgctctca gatcgtcgta
    12781 cttcacgggc gcctcgtcgg cctgcctgtc aatgaaaccc tgaatcttct ccggaattgg
    12841 catggggaac atcctctccg caggggtgat ggccgtcaag ccgacgccga ccgcaactag
    12901 cgaacgcggc gcgttatcta gatacagaag gtcagtagcg gtcaccctcc ccccacccct
    12961 ccaggagcag tcttgagacc ccagtcgaac tccaggttcg cgcttttcat tcttccggca
    13021 ctcgtcgcgg cacttttcgc cgtgctctcc gcgggggcgc aggcggcgag tccgctttcg
    13081 atcgaggtgc tctcgagcaa ggccgaccgg atttccggcg gtgacgcgct ggtcgccgtc
    13141 acggctccgg actcgagcgc gaagagcaaa atggtcgtcc ggctgaacgg gaaggtcgtg
    13201 acttcctccc tcgccagcga tcccgacaac tcgctccgca agaagggcct cgtctccggg
    13261 atgtggaacg gccgcaactg gatcaccgcc tgggctccgg gccaggattc ggcgacccgc
    13321 ctttcggtct tcaacagctc gcttcagggg ccggtcttct ccgggcccca ccagaccccg
    13381 tactactgca cgaccgagag tttcggactc ggcccggcgc aggattcgaa ctgcgtcgcc
    13441 ccgaccaggg tctcctggta ctaccgcgcc agcgacaaca ccttcaagcc gctgaccagc
    13501 ccggccgacc ggcccgcgga catggtccag accaccaccc gcacgggtca actggtcgat
    13561 tacgtggtcc ggctcgagca gggtgtgatc aaccgtgcgg tctacaactt cgcgacgctg
    13621 gcctccggcg gccagacggg taacggctgg aacggccgtt tcgtctatca gtacggcggc
    13681 ggttgctcga ccggccacca gcaaggcgcg ggagtgccgg atccgctcga cgatggcctg
    13741 ctgaagcggg ggttcgtggt cctcaactcg agcctcaacg tcttcaacac gacctgcaac
    13801 gacgtgatct ccgccgagac cacgtcgatg atcaaggaac gcgcgatcga ggaactcggc
    13861 cgggccgaca cctggaccat cggccagggc agctccggcg gctcggtcca ggtccagatg
    13921 atcgcccaga actacccggg acttctcgac gggctgaatc cgggggcgag ctttcccgac
    13981 aactcctccc ccgattaccc cgactgccgt ctgctggtca actacttcaa caacacgacc
    14041 accggccagg acctctcgaa cgcccagaag cgacagatca ccggcatgac ctcggacacc
    14101 aacggcggct gtgaaccgct ttcggccggc gccgacgtca ttcgcgccca ggaaggctgc
    14161 aacgaggatg tggtccccgt ctccgtgatc ttcgatcccc tgtccaatcc gggcggcgtc
    14221 cgctgcacgc tctgggacaa catgatcaac gtctacgggg ctgacccggc caccggctac
    14281 gcccgccgca cgctcgacaa cgtcggggtg cagtacggcc tcggggcgct taagggcgga
    14341 tcgatcaaca tgaagcgatt cctcgacctg aacgagcaga tcggcggata cgacaacaac
    14401 ggcaacttcc aggccggtcg aaccgtggct gaccaggatg cgctgaagac cagctaccag
    14461 accggccgca tccagcaggg agccggggga tggacccagg tgccgatcat cgacgtccgc
    14521 aactactagg acgacgtagc ggccggcaac gtccatcaat acgtcaacac ctaccgcacc
    14581 cgggcccggc tcgaccgctt caacggaaat cacgacaacc aggtcatgtt ccgggcgatc
    14641 ggcggcccca gcgtgaaggc gatgaatgac acggcgatcg acacgctctc cacctggctc
    14701 gactcgatcg tcgccgatga ttcttcgaag tcactggccc agaaggtggt cgacaacaag
    14761 cccgccgacg cggtcgacgc ctgctggatc gccggcaacc gggtcgacgg cgtcgcccag
    14821 gccggtgaca cgaacacctg cgagacgacc tacccgccgc attcgctacc ggtgaacgtg
    14881 gccggcaagc cgctcgattc gattaccgcc aagtgcaccc tcaaaccggt caatccggcc
    14941 gattacggct cacccacccc ggagcagata acccgcctca accagatctt cccggacggg
    15001 gtctgcgact ggtcgcaggc gggaccggaa gagaccaccg tccagggcac cgatctctcc
    15061 ttcgggcctt cccatgaggc cggcgatgcg aaccgccacc tgggcctcaa gttgaaccgt
    15121 tacaagctca actacagccg caagggcggc agcgtcacgg cgaccgcgac catgggcaac
    15181 tgccccgcgg tcacctggca gaaagtccgc ttcgagcgac aggtcaggca gggacgcaag
    15241 tgggtctgga agaacatcgg ttcgaagatg gtcaccggaa aggattgcca ggcctcgctc
    15301 acggtcagga acatcaaggg caagacgaac ctgcgggccc gggcgctggc gatcaccggc
    15361 ttcaaggccg cgaactcgaa gctccgcacg gtcacggtga agaagccgaa gcgtcaccgt
    15421 cgctgaccgg cggggcgtcg cctgagaccc ttccccggat atactccgcc gacttcgcgg
    15481 ctgtagctca gttggctaga gcgtctgctt gccatgcaga aggtcgaggg ttcgaatccc
    15541 ttcagccgct tccgggacac cccttgcggg gcattcccat tcggcgcctt ggcggagtgg
    15601 ctacgcagcg gcctgcaaag ccgtttacac cggttcgact ccggtaggcg cctttctgcc
    15661 ggcgtccctg aacggtgcgg attgaaaagc gaggcggacg gtcggctgcc cgcccctctt
    15721 tccgtccgga attcccggtc tacttgaccg tgaagtccgt gtacatacca gccttgtagt
    15781 ggccgggcag gttgcagatg agcgcgtagt ggccggcagc gagcttcagg tcgagttctg
    15841 aactctcgcc ggcctgaact tcggtctctc caaccgaacc ggtctcgtcc gctttcccgg
    15901 aagcgtcggt aggcagatcg cccgccttct tgttcgtctt gaccactacc agctcgtgct
    15961 cgatgttccc gtcattggtc acgtcgaagg ccacgtcccc ggccttcacg gagtccggcg
    16021 aagcgctgat cgccatctcg gtcaaactga tcttgacgga ctgggcgttg gccggagcct
    16081 gggtagtcga ggttgtggtg ccggtgtcgc tgctctctcc ggaatcatcg ctcgaggatc
    16141 cacaagcgga gattcccagg gcaagaacaa gcgttgccgc agccacagat acacgcaatg
    16201 cgtttggctt caaagggagg cctttctgac taggttcacc aggacggaag acctggcgac
    16261 gcttgagttt acgcctggga gtcgagggcc tggttgacca gcgcgtcggc ttcggcgttc
    16321 tgttcacggc ggacgtgact gatcgaccac gagtcgaggc cttcgagcgc ggccttggct
    16381 tcggcgtgga gcggcttcat gtccggatgc ttgaccttgt actgaccgag catctgcttg
    16441 acgaccagtt ccgaatcacc gtggatctga agttcggtcg cgccgagttc ccgggccagg
    16501 ccgatgccct tgatcagggc cctgtactcg gccacgttgt ttgtggcttc gccgatcgtc
    16561 tc
//
LOCUS       DAOQKN010000033        16408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_1613_length_16408_cov_4.995353, whole genome shotgun sequence.
ACCESSION   DAOQKN010000033 DAOQKN010000000
VERSION     DAOQKN010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 16408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16408
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1613_length_16408_cov_4.995353"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..846)
                     /locus_tag="PLJ64_03745"
     CDS             complement(115..846)
                     /locus_tag="PLJ64_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative dsRNA-binding protein"
                     /protein_id="HNC92635.1"
                     /translation="MSWLKRSRERAGGHDPVTPDSGLASLIEGLPEDLKVPALTHSSW
                     TSERTDSYERLALLGDSVLGLAVADELYRTLPGLSAGALTKILNQTVSGTSCAEVGRE
                     LGIPDMLLAADPGEPDGKQTPARLLLEGERPLPEITEALIGACYLHYGFGATSSAVIE
                     AFRPRISLVRENQTDFKSALQEEAARHGETVSYEVIAAHGPAHQRTYEVAVIFREEEI
                     GRGEGRSKKAAGQAAAEVALERLGD"
     gene            complement(821..1066)
                     /gene="acpP"
                     /locus_tag="PLJ64_03750"
     CDS             complement(821..1066)
                     /gene="acpP"
                     /locus_tag="PLJ64_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNC92636.1"
                     /translation="MNRDEVMDLVRNHLVTELELGPEQITETARFKEDFDADSLDLYE
                     LVMELEDQYGITVSEQEAAEIKTVGDALDFVVAKAVA"
     gene            complement(1079..2104)
                     /locus_tag="PLJ64_03755"
     CDS             complement(1079..2104)
                     /locus_tag="PLJ64_03755"
                     /inference="COORDINATES: protein motif:HMM:NF014554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HNC92637.1"
                     /translation="MDLSGTTVALDGLGVESGFDVLNEGIRLAAGDGIRLRVFGPADA
                     IEVSGLPGVEVIDCTDQITNDDDPVPSVRSRPDSSIVRAVADVGAGNSESVVSLGSTG
                     ATMAASTFGLKRLKGVKRPALAAQVPLPGRTVLFLDIGANVEVRAQHLVQFAWLGAAF
                     SKSVLGVERPEVGLLSVGEEAGKGREEVVEAHAALVGAGEFDFIGNVEGRDLPAGNAD
                     VIVTDGFTGNVVTKALEGTVKRVVGEISAAARKNPLSAAGGLLLKPALGGLRHDLHPD
                     TTGGAILLGLRKTAVVGHGSSGSDGVANAIRLAARSARVDAPGLTEEMLHRVGVNRGS
                     IDSATDV"
     gene            complement(2114..2323)
                     /gene="rpmF"
                     /locus_tag="PLJ64_03760"
     CDS             complement(2114..2323)
                     /gene="rpmF"
                     /locus_tag="PLJ64_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008678357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNC92638.1"
                     /translation="MAVPKKRTSRTRRDKRRATHKAAKARVNHCPHCHHPRLPHRVCA
                     NCGQYKGREVVAPKEQPFGTVTPDA"
     gene            complement(2392..2961)
                     /locus_tag="PLJ64_03765"
     CDS             complement(2392..2961)
                     /locus_tag="PLJ64_03765"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HNC92639.1"
                     /translation="MLIEKVDLDALGLSYGDATRIQGEVAPARVSLGGQDYEPVPAEP
                     AFQLEVSRTSAGYALRLRFEVGLSGPCFRCLEDATASVSVDAREVDQPVEPIPIQGSG
                     RIQKDDEDEDESSAAELTSPYVEEGTLDLAAWTHDALILSLPEQILCQPDCLGLCPVC
                     GESLNGADPAAHDHGQDIDPRWAKLQDLK"
     gene            2998..3537
                     /locus_tag="PLJ64_03770"
     CDS             2998..3537
                     /locus_tag="PLJ64_03770"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC92640.1"
                     /translation="MSQPDESSYLDSVRGQVPLYDALQDAAVAAVPFEPSRVLELGIG
                     TGETASRLLARFPDANVTGLDSDPDMLFKAREIVDEVSLARMEDPLPDGPWDLILSVL
                     SVHRLTDEQKQTLFRRVREQSKALVIGDFVSLGDDRGETRVEGEAYPDTAEDLAAWCD
                     GKIVWSEDDLVVIVAEYRR"
     gene            complement(3626..4096)
                     /locus_tag="PLJ64_03775"
     CDS             complement(3626..4096)
                     /locus_tag="PLJ64_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HNC92641.1"
                     /translation="MDVLVLIDKLDDLVHNARPVPLTDQVRVDREEIYDLLDQMRATI
                     PEEIKQARWIVKERQEMLEEAKRESERVIREAREQQARLVSDEEVTKQAERAADEIVE
                     DARAREREIRLGAEDYADEILNTLEVNLQKFTAAVQRGRDRLAGRDEPLDEIEE"
     gene            complement(4200..4703)
                     /gene="coaD"
                     /locus_tag="PLJ64_03780"
     CDS             complement(4200..4703)
                     /gene="coaD"
                     /locus_tag="PLJ64_03780"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNC92642.1"
                     /translation="MNDRTERVAVCPGSYDPVTNGHLDIITRASRTFDKVVVGVVNQP
                     IRKKKTLFSAEERIAFIENATGHLGNVEVQAFYTLLVEFARENGASAIVKGLRAISDF
                     EYENEMAQLNYKLDPGVESIYVFARPEYSFLSSTGVKEMATFNGDISDLVPAEVATAL
                     ADRLGRS"
     gene            complement(4700..5260)
                     /locus_tag="PLJ64_03785"
     CDS             complement(4700..5260)
                     /locus_tag="PLJ64_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HNC92643.1"
                     /translation="MRVIAGRLGGRQLLAPKGWKVRPTSERVREAIFSALGDIGGMRV
                     ADLYCGTGALGIEAISRGAGPAVLVDRETRPAMGNVHNLGLTGEAELVRAEAPEWIAN
                     VPDGSFDLVFLDPPYRDATRIAGELDPHLARILAPGGRVIAESDRRGPLEFPSLETVR
                     ERRYGRTVVTFHRVPSQPPQDPTGTE"
     gene            complement(5257..7371)
                     /locus_tag="PLJ64_03790"
     CDS             complement(5257..7371)
                     /locus_tag="PLJ64_03790"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNC92644.1"
                     /translation="MSWPDPGILDRELTRLSGVGAKLGAAAAKAGVGTIGDLLWRLPR
                     AYGRRPGTSLLGDLEPGEATGVEVRVLRSRRVRTRRRRFSVVEARIADDSGARKAVWF
                     NQPWMAEKLTVDSTWYLEGRLEKGGFVVSASEPVGGPAVEAGSGQGIKWQSAGSGPPG
                     LADDSTRATHSAGGEIGPGRWRRWAFEACRMAAGLPEPLPARLLRSRRLPGLAAAFRE
                     AHFPVDEERARTALRRLAYQELIENQAVIRDRRIRHRRGSGPATSLEGGRKLADRWLG
                     GLPFQATGDQSSAIARIAGDLAATEPMGRLLMGEVGSGKTVVAVHAMLQAVGSGAQAA
                     MMAPTEVLATQHFITLSDMLEGTGVEVALLTGSTSAADRKEMLRRLAEGRIAILVGTH
                     ALLEGPVRFRQLALAVIDEEHRFGVRQRAGLDAKAPAGHAAHLLHLSATPIPRTLALT
                     AYGDLDVTTLKELPAGREPVQTEVVSAGGRAMAFERLKAELERGRQAFVVCPLVEEST
                     LVEGRAAAAEAERLAASELAGYEVGLLHGQMKPAQKEVAMAAFADGRIDVLVATTVIE
                     VGIDVPNATVMVIEGADRFGLSQLHQLRGRIGRGSHGGTCFLFPESSGARAAKRLGAM
                     ASTSDGFRLAELDLEMRGEGEVAGTRQHGLPRFRVASLPRDIELLEAVRQDLGSMNET
                     ELGMLGELIRNPSEAAEPGVAA"
     gene            complement(7461..9098)
                     /locus_tag="PLJ64_03795"
     CDS             complement(7461..9098)
                     /locus_tag="PLJ64_03795"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HNC92645.1"
                     /translation="MSDPSIERFKRVVAAAYGSLEARRQEVNDLNVFPVADGDTGDNM
                     AMTMKAVMEELDSFENGQSLDEIGRTELVSALARAALMGARGNSGVILSQIVRGAAEE
                     LASRPGELVDPVLVASAFGKAADAAYASVRNPAEGTMLTVFREIAHSVSRQLAHIEPE
                     QQRLAKDATPAEQDQVLADVLEQAIADGQKAVERTPEQLEVLAQSGVVDAGGYGLVLI
                     LAGVLAALRGEDADLPEIQHHSAAQITHPEHEDSRFQYCTNFIVSGSGLNSRQFIGRL
                     EEIGDSVLVVGDESTLKVHVHTDDPEAAMEVFVEAGAVTNLDVADMRKQIAEREARIN
                     ADHRTGVVVVGSGDGLESLFTEMGATVVAGGETMNPSTAELLAGIRAVPVEEVLVLPN
                     SSNVIMAAERACELSEKRAVVLPTRTQQEGLLALIEFNGESDLDTNRRRLEEAVAEVT
                     VGGIAPAARDDAQGRFRRGDAVGFISGEIVAWGGAGSALASTIERLADEAELVTVIAG
                     EGAPIPLDEIDDHSPNGVELELHEGGQPSWWWLLASQ"
     assembly_gap    9220..9319
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9397..9588)
                     /gene="rpmB"
                     /locus_tag="PLJ64_03800"
     CDS             complement(9397..9588)
                     /gene="rpmB"
                     /locus_tag="PLJ64_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNC92646.1"
                     /translation="MARVCHNCGKGPGFGNSRSHSMVATRRRFDPNLQKVRILEGGAK
                     KRVLVCTRCLKANKVTKSA"
     gene            complement(9661..10116)
                     /gene="ribH"
                     /locus_tag="PLJ64_03805"
     CDS             complement(9661..10116)
                     /gene="ribH"
                     /locus_tag="PLJ64_03805"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNC92647.1"
                     /translation="MPEPEFTGRFCVVVGNFYTDLADRLVGGAKEEFARDGISDAEID
                     VYEVPGAFELPLAAGYAARSGRYDGVACLGAVIRGETDHYDFVCNEAARGIQNVQLET
                     GVPCGFGVLTVDNMEQALARAGGGGKRDSGANAARAVLRMNEIRRELSA"
     gene            complement(10109..11416)
                     /locus_tag="PLJ64_03810"
     CDS             complement(10109..11416)
                     /locus_tag="PLJ64_03810"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HNC92648.1"
                     /translation="MSTTEATPFATIEEALEEIRAGRMIIVTDDEDRENEGDLVMAAQ
                     FATPEAINFMAKEGRGLICLSLTAQRCDQLGLRLMTAKNEAPLETAFTVSIEAAEGVT
                     TGISAHDRAHTVQVAIDPDATPRDIVVPGHVFPLKAKDGGVLERTGHTEAAVDLARLA
                     GVSPAGVICEVMNEDGTMARVDDLVGYAEKHGLKMVTIADLIAYRRENEKLVERVVST
                     ALPTSFGDFRAVGFRSLVDDKHHVAMVKGEIDTGEPALVRVHSECLTGDVFHSMRCDC
                     GEQLAAAMAMIEDEGRGVLLYLSQEGRGIGLLNKLRAYKLQEEGLDTVDANLKLGLPA
                     DLRDYGIGAQILRDLGLTKIKILTNNPKKIIGLEGHGLEVTEQIKIEAPPNTHNESYL
                     RTKRDKMGHILHHQGLALDEEMILDEQIHDSEKATEAEGGRGA"
     gene            complement(11472..12089)
                     /locus_tag="PLJ64_03815"
     CDS             complement(11472..12089)
                     /locus_tag="PLJ64_03815"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNC92649.1"
                     /translation="MFSGLVQDLGEVKSIDLSSEGARISVATTLAGEIGLGDSVAVNG
                     CCLTATAVDAGGFTADAMQQTLDLTSIGGLEAGSPVNLELALKASDRLGGHIVQGHVD
                     GTAGVIGREQDGFSLRLRISLPAELSRYVIPQGSITIEGVSLTVADCGEDWAEVALIP
                     ETRERTNLGTLQIGTTVNIECDVIAKYVERMVSPYAPGSESHTDR"
     gene            complement(12089..13219)
                     /gene="ribD"
                     /locus_tag="PLJ64_03820"
     CDS             complement(12089..13219)
                     /gene="ribD"
                     /locus_tag="PLJ64_03820"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HNC92650.1"
                     /translation="MTAAQPTSEADYWQRAIQLAEHGLGWTSPNPAVGAVLVKDGRVI
                     GEGWHRRHGELHAEREALADAERRGNDPAGATIFVTLEPCAHTGSQPPCADGLIEAGV
                     GEVVIAAEDPTEKTRGVGPARLEDAGIRVRWAEGPAADAALALIQDFRKRALTGKPLV
                     TLKMAMSLDGKVATRTGDSKWISGEESRALVHRWRAEMDAVAVGSGTLTADDPRLTAR
                     HTDGEPVRQPARVIFDSGLLITTGAALFADIDEAPLLIIAGPDPDPDRLAALETAGAE
                     VIPVVGEGSGARFAAAMEALGERGIGSLLLEGGPRLAGAALTAGEVDRTEIFIAPLIL
                     GGGRPATEGSGPELMEGATRVPKMRVSRVGQDVLMSSTIRTW"
     gene            13238..14236
                     /locus_tag="PLJ64_03825"
     CDS             13238..14236
                     /locus_tag="PLJ64_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNC92651.1"
                     /translation="MKVIIQIPCLNEEETLPATLADLPREIEGVDEIETLVIDDGSTD
                     RTIEVARQCGVDHIVKLPNNRGLATGFQSGLDACLKLGADIVVNTDADNQYSGSDIPK
                     LVAPILAGDAEMVVGDRKVAEIDHFSPAKKVLQKFGSWVVRQLSNTEVTDTTSGFRAY
                     NREAALQLLVVDNFTYTLESLIQAGKGNVAVGEVTISTNEKTRESRLFGSTSAYVRRN
                     GLAILRIYTRYEPLKVFSLLGLIAAVAAIAAWMPFLIDWIVNGDRDGHIQSLLLGAVL
                     FIVSIQLFALGIIGDLLAGQRVMTQRVYERVRRIELELEVEPSHRLDPPRDRSAVQ"
     gene            14241..15668
                     /locus_tag="PLJ64_03830"
     CDS             14241..15668
                     /locus_tag="PLJ64_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92652.1"
                     /translation="MSDSPEKTESSTRAYGRSVGLLTIGVGLTGIFTYAYFLIASHDL
                     SKDAYGEITVLWSAVFITVSTLYRPVDQLLSRHISEHIERGETDVGPVRVAAKIQICL
                     AIGFAITALILKKPLEDGLLAGNETLYWVFFSSVLFYAASYFARGYLAGHQQFGLFTT
                     LILSESVFRTLFALLVAFGLLSGQSWVAMGIVAAPLLSLMVVPSAITRRSRREAEERA
                     RVAEQRGTAPEDFDAEVAEAREEFSLSHGAGFVGSVFLIMFSEQVFLNAGPLVVRAVE
                     GVAAAGYIFNVLMIARAPLQLFQAVSTSILPHLTALHHSSDPNSEKEFHHSVRMVLLG
                     IAAFTALTAVVVMIAGPKLMQIAFSDKFTYDRLGLMLVTAGMGLYLAAVTVNQACLAQ
                     GQARRSSIRWISCAVFFIIWTILPFISDGNLRVEIGFALTALLLLSLLTFIYFRPKGV
                     EADVPLEGSSEAIEARLAGLEDTST"
     gene            complement(15751..>16408)
                     /gene="rpe"
                     /locus_tag="PLJ64_03835"
     CDS             complement(15751..>16408)
                     /gene="rpe"
                     /locus_tag="PLJ64_03835"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNC92653.1"
                     /translation="RIAPSILSADFGRLGSQIAEIMDAGARVIHFDVMDGQFVPPITI
                     GPLVLSGIADQVHDAGGVVDVHLMIEDPDRQFEDFARAGADSIIFHEEATPHAHRCCH
                     AIRELGCLAGVAINPGTPVETVSELKGMVDEILVMTVNPGWGGQPFIETSPDKVRRMR
                     SLVGDDVLIEVDGGIGTGTAGTMAAAGATLMVAGSAIFGSDDPAQAYRDIHAAAESGA
                     "
BASE COUNT         2553 a         5539 c         5255 g         2961 t
ORIGIN      
        1 atacatgcgc cttacagatt gccggttccg ccggacggac gattccccac cccggaacgc
       61 ctcaacaccg cagcccgact gggaaaaacc gtttcccgca aagtgcaggg agtttcagtc
      121 gccaagcctt tcgagggcga cttcggcggc cgcctgaccg gctgctttct tgcttctgcc
      181 ttcacccctc ccgatctctt cctcacggaa gatgacggct acctcgtagg tcctctgatg
      241 ggccggaccg tgggccgcga tcacctcgta ggagacggtt tcaccgtggc gcgcggcctc
      301 ttcctgaagg gccgacttga aatccgtctg gttctcccgg accagggaga tccggggccg
      361 gaaggcttcg atcacggcac tcgaagtggc tccgaatccg tagtggaggt aacaggcgcc
      421 gatcaatgct tcggtgattt ccggcagcgg ccgctcgccc tccagcagca ggcgggccgg
      481 ggtctgcttg ccgtcaggtt cgcccgggtc cgcggccagc aacatgtccg ggatgccgag
      541 ctcgcgaccg acctcggcgc aggaggttcc actgaccgtc tggttgagga tcttggtcag
      601 ggcccccgca ctcagcccgg gaagggtgcg gtaaagctcg tccgcgacgg ccagtccgag
      661 gacgctgtca ccgagcaggg caagccgttc gtatgagtct gtccgctcgc tggtccagga
      721 ggaatgggtc agggccggaa ccttgaggtc ctccggcagg ccttcgatca ggctcgccag
      781 gcccgaatcc ggggttaccg ggtcgtgccc cccggcacgc tcacgcgacc gctttagcca
      841 cgacaaagtc aagcgcgtcc ccaacggtct tgatttccgc ggcctcctgt tcggagaccg
      901 tgatcccgta ctggtcctcg agttccatca cgagttcata gagatcgagt gaatcggcgt
      961 cgaagtcttc cttgaacctc gccgtttcgg tgatctgttc gggtcccagt tccagttcgg
     1021 tgaccaggtg gttgcggacc agatccatta cttcgtcgcg gttcattccc gtttcctatc
     1081 agacgtcagt tgcggaatcg atcgagccgc ggttaacgcc gacccggtga agcatctctt
     1141 cggtcagccc gggggcgtcg acccgggccg accgggcggc caggcggatg gcgtttgcca
     1201 ccccgtcgga gccggagctg ccatgcccga ccacggctgt ctttcgcaat ccgagcagga
     1261 tcgctccgcc ggtcgtgtcg ggatgaaggt cgtgccggag accgcccagc gccggcttga
     1321 gcagcagccc gccggccgcc gacagggggt tcttccgggc cgcggcggaa atctcgccga
     1381 ccacccgctt gaccgtccct tccagggcct tggtcaccac gtttccggtg aaaccatcgg
     1441 tcacgatcac gtcggcgttc ccggccggca ggtcgcggcc ttcgacgttg ccgatgaagt
     1501 cgaattcccc ggctccgacc agggcggcat gagcctcgac gacttcctcc cggcccttgc
     1561 ccgcctcttc gcccaccgaa agcagcccga cttcgggccg ctccacaccg agcaccgact
     1621 tcgaaaacgc ggcgcccagc caggcgaact gcaccaggtg ctgggcccta acctcgacgt
     1681 tcgcgccgat gtcgagaaag agaacggtcc gtcccggcag cggaacctgc gcggccaggg
     1741 ccggtcgttt gacccccttc agccgtttca ggccgaaggt tgacgccgcc atcgtcgccc
     1801 cggttgatcc gaggctgacg accgattccg aattcccggc acctacatcg gccaccgccc
     1861 ggacgatcga ggagtcggga cgcgaccgca ccgaggggac gggatcgtcg tcgttggtga
     1921 tctggtcggt gcagtcgatc acctcgaccc ccggcaaacc ggaaacctcg atcgcgtcgg
     1981 ccggcccgaa aaccctcagc cggattccgt ctccggcggc aagccggatt ccttcgttca
     2041 ggacatcgaa gccgctttcg acgccgaggc cgtcaagcgc gaccgtagtc ccgctcaggt
     2101 ccaagagttc aggctaggcg tcgggggtga cggtgccgaa gggctgctct ttgggagcga
     2161 cgacctcacg gcccttgtac tggccacagt tcgcgcagac gcgatgcgga aggcgcggat
     2221 ggtggcaatg cgggcagtgg ttgacgcggg ccttggcggc cttgtgggta gcgcgccgct
     2281 tgtcccggcg ggtccttgag gttcgtttct tcggtacggc cataagacgc agcagtctag
     2341 aggaacggcg gctgaagtga catccacccg cccgggatgg ctcccctcct gctatttcag
     2401 gtcttggagc ttcgcccagc gggggtcgat gtcctggcca tgatcatgcg cggcggggtc
     2461 ggccccgttc agggactctc cgcaaaccgg gcaaagaccg aggcaatcag gctggcagag
     2521 gatctgctcc ggcagggaga ggatcagggc gtcgtgggtc caggcggcca ggtcgagggt
     2581 gccttcctcg acataggggc tggtcagctc cgcggcgctg gattcatctt cgtcctcgtc
     2641 gtctttctgg atccggcccg aaccctggat cgggatcggt tcgaccggct ggtcgacctc
     2701 ccttgcgtca accgaaacgc tggcggtcgc atcttccagg cagcggaaac agggcccgga
     2761 aaggccgacc tcgaagcgga gcctgagtgc gtagccggcg ctggtccgcg agacctcgag
     2821 ctggaaggca ggttcggccg gcaccggttc gtagtcctgc ccgccgaggg aaaccctggc
     2881 cggagccact tcaccctgga ttcttgtggc gtctccatag gagaggccga gggcgtcgag
     2941 atcaaccttt tcgatcagca tcccttccca gcatggcaga cgaatacgat gcgctcgatg
     3001 agccagccgg acgaaagcag ctacctcgac tcggtccgcg gccaggtgcc gctttacgac
     3061 gcgcttcagg atgcggcggt cgccgcggtc cccttcgaac cgtcccgggt tcttgaactg
     3121 ggaatcggca ccggtgaaac cgcttcgcgt ctgctggccc gttttcccga tgcgaatgtc
     3181 accggcctgg attcggatcc cgacatgctc ttcaaggccc gggaaatcgt cgacgaggtc
     3241 agtctcgccc gcatggagga tccgctcccg gacggtccgt gggacctgat cctctcggtc
     3301 ctttcggtgc atcggctgac cgacgagcag aagcagaccc tgtttcgccg ggtccgcgag
     3361 cagtcaaagg ccctggtcat cggcgatttc gtctcgctgg gcgacgaccg gggagagacc
     3421 cgggtcgaag gcgaggccta ccccgacaca gccgaagacc tggccgcctg gtgcgacgga
     3481 aagatcgtct ggtccgaaga cgacctagta gtcatcgttg cggaataccg aagatagagc
     3541 cccacccaac cattccttga ttcgaatggg tggggcgggt tgctttggga gggccggcgc
     3601 agaccggccc gactgcagtt aagtcttatt cctcgatttc gtcgaggggt tcgtcgcggc
     3661 cggcaagcct gtcgcgaccg cgctggacgg cggcggtgaa cttctggagg ttcacttcga
     3721 gggtgttgag gatctcgtcg gcgtaatcct cggcaccgag acggatctcg cgctcgcggg
     3781 cacgggcgtc ttcgacgatt tcgtcggcgg cccgctcggc ctgcttggtg acttcctcat
     3841 cggaaaccag ccgcgcctgc tgctcgcgtg cctcgcggat tacccgctcg gattcacgct
     3901 tggcctcttc gagcatctcc tgacgctcct tgacgatcca gcgcgcctgt ttgatctctt
     3961 ccgggatcgt ggcacgcatc tggtcgagca gatcgtagat ctcctcgcgg tccacgcgca
     4021 cctggtcggt aagcgggacc ggacgggcgt tgtggacgag gtcgtcgagc ttgtctatta
     4081 gaacgaggac atccatgtgg agggaaccct atgcgtctcc gtcgatttgc gggggaaatg
     4141 tggccatgtg gtgaagattc tgcatggaat cttccattcc tctttgtgac atagaggcac
     4201 taactgcgac caagtctatc cgccagtgcg gttgccacct cggccgggac cagatcgctg
     4261 atgtcgccgt tgaaggtcgc catttccttg accccggtcg aggaaaggaa gctgtactcc
     4321 ggccgggcaa agacgtagat gctctcgacc ccgggatcga gcttgtaatt gagctgggcc
     4381 atctcgtttt cgtactcgaa gtccgagatc gcgcgcagcc ccttgacgat cgcactggcg
     4441 ccgttctccc gggcgaattc gaccagcagg gtgtagaagg cctggacttc cacgttgccg
     4501 agatgaccgg tcgcgttctc gatgaaggcg atccgctctt cggccgagaa cagggtcttc
     4561 ttcttgcgga tcggctggtt gacgactccc accacgacct tgtcgaaggt gcgggaggcg
     4621 cgggtgatga tgtcgaggtg gccgttggta accgggtcgt agctcccggg gcagacggcg
     4681 actctctcgg ttctgtcgtt cattcggttc cggttgggtc ttgagggggt tgggacggga
     4741 cgcgatggaa tgtaaccacc gtgcggccgt agcgtcgttc ccgaaccgtc tcgagggacg
     4801 gaaattccag tggtccgcgc cggtcagact cggcgatcac acggcccccc ggggcaagga
     4861 tccgggccag gtgggggtcc agttcgccgg cgatccgggt cgcgtcccgg tatggcgggt
     4921 cgagaaagac caggtcgaaa ctgccgtcgg gcacattggc gatccattcc ggcgcctcgg
     4981 cccggaccag ctcggcctcg ccggtcaagc cgaggttgtg gacgttgccc atcgccgggc
     5041 gggtttcacg gtcgaccagc acggccggcc cggccccccg tgagatcgcc tcgataccca
     5101 gcgctccggt tccgcagtag aggtccgcga cccgcatgcc cccgatgtcg ccgagtgccg
     5161 agaagatcgc ctcccggacc cgctccgagg tcggccggac tttccatcct ttgggggcga
     5221 gcagctggcg gccaccgagc cgtccggcga tcaccctcat gccgccaccc ccggttcggc
     5281 cgcttcggag gggttgcgga tcagttcgcc cagcattccc agttcggttt cgttcatcga
     5341 accgagatcc tgacggaccg cctcgagcag ctcgatgtcg cgaggcagag aggcaacccg
     5401 gaaccttggc aggccatgct gcctggttcc tgcgacctcg ccctcccccc tcatctccag
     5461 gtcgagttcg gccagccgga atccgtcgct ggtcgaggcc attgccccca gccgcttcgc
     5521 agcccgggcc ccggaactct ccgggaagag aaagcaggtg ccgccgtggc tgccgcggcc
     5581 gatcctgccg cgaagctggt gaagctggga gagtccgaag cggtccgctc cctcgatcac
     5641 catcacggtg gcgttgggca cgtcgatccc gacctcgatc accgtggtcg cgaccagcac
     5701 gtcgatccgc ccgtcggcga aggccgccat cgcgacctcc ttctgggccg gtttcatctg
     5761 gccgtggagc agcccgacct cgtagccggc aagctcgctg gccgccaggc gttcggcctc
     5821 ggctgcggcg gcccggccct cgaccagggt cgactcctcg accagcgggc agaccacgaa
     5881 tgcctgccgg ccccgctcca gttcggcttt gaggcgctcg aaagccatcg ccctcccgcc
     5941 cgccgacacg acttcggtct gcaccggttc ccggccggcg ggaagttcct tcagggtcgt
     6001 cacgtcgagg tcgccgtagg cggtcagggc cagggtccgg gggatcggcg tggccgaaag
     6061 gtgaaggaga tgggcggcgt gtccggccgg tgccttggcg tcgaggccgg cccgctgcct
     6121 gaccccgaag cggtgttcct cgtcgataac cgcgagggcg agctgcctga agcggaccgg
     6181 accttcgagc agggcatggg tgccgaccag gattgcgatt cggccttcgg ccagccgccg
     6241 gagcatttcc ttccggtcgg ccgcgcttgt cgagccggtc agcagtgcca cctccacccc
     6301 tgtcccctcg agcatgtccg agagggtgat gaagtgctgg gtggcgagga cctcggtcgg
     6361 ggccatcatc gccgcctggg caccgctgcc gaccgcttgc agcatcgcgt ggaccgcgac
     6421 caccgtcttg ccggagccga cctcgcccat cagcaggcgg cccatcggct cggtcgcggc
     6481 gagatcgccg gcgatccggg cgatcgcgct tgactggtcc cccgtcgcct ggaagggcag
     6541 cccgcccagc cagcggtcgg ccagcttacg gccgccttcg aggctcgtgg ccggcccgga
     6601 gccacgccgg tgccggatgc gccggtcccg gatcacagcc tggttttcga tcagctcctg
     6661 gtaggcgagc ctcctcagcg ccgttctcgc ccgctcctcg tccaccggga aatgagcctc
     6721 ccgaaaggcg gcggcgagcc ccggcaaccg ccggcttcga agcagccgcg ccggcagcgg
     6781 ctccggcagg ccggcggcca tccggcaggc ttcgaaagcc cagcgccgcc agcgtcccgg
     6841 cccgatctcg ccgccggccg aatgagtggc ccgggtcgag tcatcggcaa gacccggcgg
     6901 accagacccg gctgactgcc acttgatccc ctggccggaa cccgcttcga ccgctggccc
     6961 gccaaccggc tccgatgccg aaaccacgaa accgcccttc tccaggcggc cttcgaggta
     7021 ccaggtgctg tcaacggtca gtttctcggc catccagggc tggttgaacc agaccgcctt
     7081 gcgcgcaccg gagtcatccg cgatcctcgc ctccaccacg ctgaacctgc gccgtctggt
     7141 tctgacccgg cgggacctca gcacccggac ctccaccccg gtcgcttcac ccggttcgag
     7201 atccccgagc agactcgttc cggggcgccg cccgtaggcg cgcgggagcc gccagagcag
     7261 gtcgccgatc gtcccgaccc cggccttggc ggccgccgcc ccgagtttcg cgccgacccc
     7321 gctcaaccgg gtcagttccc ggtccagaat gccggggtca ggccagctca cgggggcggc
     7381 gagcaagctg tttcggtccg ggtccggttc cggaccgccg ccgaatccgg gcggaggcaa
     7441 aggcggcccg gaagccgcgg tcactggctg gccagcagcc accaccagct cggctgcccg
     7501 ccctcgtgaa gttcgagttc aactccgttc gggctgtggt cgtcgatctc gtccagcggg
     7561 atcggtgccc cttcgccggc aatcaccgtg accagttcgg cctcgtcggc gaggcgttcg
     7621 atggttgagg ccagcgccga tccggcgccg ccccaggcga cgatctcgcc ggagatgaaa
     7681 ccgaccgcgt ctccgcgccg gaacctgccc tgtgcgtcat ccctggccgc cggagcgatc
     7741 ccgccgaccg taacctcggc gacggcttct tccagccggc ggcgattggt gtcgagatct
     7801 gactcgccgt tgaattcgat cagggccagc aggccctcct gctgggtgcg ggtcggcagc
     7861 accaccgccc gcttctcgga gagctcgcag gccctttcgg cggccatgat cacgttcgag
     7921 gagttcggca ggaccagcac ttcctcgacc ggcaccgccc ggatgccggc cagaagttcg
     7981 gccgtcgaag ggttcatcgt ttcgccgccg gccaccacgg tcgcgcccat ctcggtgaac
     8041 agcgattcca gcccgtcgcc gcttccgacc acgaccaccc cggtccggtg atcggcgttt
     8101 atccgcgcct cgcgttcggc gatctgcttg cgcatgtcgg caacgtcgag gttggtgacc
     8161 gcacccgcct cgacgaagac ctccatcgcc gcttcgggat cgtcggtgtg gacgtggacc
     8221 ttcagggtgg attcatcgcc gacgaccagg accgaatcac cgatctcctc gagtcggccg
     8281 atgaactggc gcgagttgag gccgctgccg gaaacgatga agttcgtgca gtactggaag
     8341 cggctgtctt catgctcggg atgggtgatc tgggcagccg agtgatgctg gatctcgggc
     8401 aggtcggcat cctcgccccg gagcgcggcc agcacaccgg ccaggatcag gaccagtccg
     8461 tagccgccgg catcgaccac ccctgactgg gccaggactt cgagctgctc cggggtccgc
     8521 tcgactgcct tctgcccgtc ggcgatcgcc tgttcgagga cgtcggcgag cacctggtcc
     8581 tgctcggccg gggtcgcgtc cttggccagt cgctgctgtt ccggctcgat gtgggcgagc
     8641 tgccgcgaaa ccgaatgggc gatctcgcga aagaccgtga gcatggttcc ctcggccggg
     8701 ttgcggaccg acgcgtaggc agcgtccgcg gccttgccga aggcggatgc gaccagaacc
     8761 ggatcgacca gctcaccggg ccgggaagca agctcttcgg ctgctccgcg aacgatctgg
     8821 ctgaggatca cgccgctgtt gccccgggcg cccatcaggg cggcgcgggc cagggccgag
     8881 accagttcgg ttcggccgat ctcgtccagc gactggccgt tttcgaagct gtcgagctcc
     8941 tccatcacag ccttcatggt catcgccatg ttgtccccgg tgtcgccgtc ggcgaccggg
     9001 aaaacattca ggtcgttgac ctcctgcctg cgggcttcaa gcgacccgta ggcagcggca
     9061 acaaccctct tgaaacgttc aatactcgga tcggacacga cgagtgagcc tatagatcga
     9121 agcggctatc ggtagtgagt cgagtggtcc gggacggcag tactaggctg aatgccgacc
     9181 gaggtgtttg ccgcttcccg gtgatgcccg cccgatcccn nnnnnnnnnn nnnnnnnnnn
     9241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9301 nnnnnnnnnn nnnnnnnnna ggctgaatgc cgaccgaggt gtttgccgct tcccggtgat
     9361 gcccgcccga tcccgcggcg tcaccacaac ggtgattcag gcggacttcg tgaccttgtt
     9421 ggccttcagg caacgagtac aaaccaggac gcgcttcttg gcgcctccct cgaggatgcg
     9481 gaccttctgg aggttcgggt cgaagcggcg ccgggtggcg accatcgagt gactccggga
     9541 atttccgaag cccggaccct taccgcagtt gtgacatacg cgtgccatgg gacggagcat
     9601 cctagccgat tcgacgagcg ggcggcctcg tggcccaccg ccggccggaa tccggcttgg
     9661 tcaggccgaa agctccctgc ggatctcgtt catgcgcagc acggcgcggg ccgcgttggc
     9721 gccggaatcg cgctttccgc cgcccccggc ccgggccagg gcctgttcca tgttgtcgac
     9781 ggtgagcacc ccgaaaccgc atgggacgcc ggtttcgagc tggacgttct ggatcccgcg
     9841 ggccgcctcg ttgcagacga agtcgtagtg gtcggtctcg ccacggatca ccgcgccgag
     9901 gcaggcgact ccgtcgtagc ggccggaacg ggccgcgtag cccgcggcaa ggggcagttc
     9961 gaaagcgccg ggaacctcat agacgtcgat ctcggcgtcg gagatcccgt ccctcgcgaa
    10021 ttcttccttc gcgccaccga ccagacggtc ggcgaggtcc gtgtagaaat tgccgaccac
    10081 cacacagaac cgtcctgtga attcgggttc aggcaccccg gccgccttcg gcttcggtcg
    10141 ccttctcgct gtcgtggatc tgctcgtcga ggatcatttc ctcgtcgagg gcaagaccct
    10201 ggtggtggag gatgtgaccc atcttgtccc gcttggtccg caggtaactc tcgttgtggg
    10261 tgttcggcgg ggcctcgatc ttgatctgct cggtgacctc gagcccgtgg ccttcgaggc
    10321 cgatgatctt cttcgggttg ttggtcagga tcttgatctt ggtcaggccg agatcccgca
    10381 ggatctgggc accgatgccg tagtcccgca ggtcggccgg caaaccgagc ttgaggttgg
    10441 catcaaccgt gtcgagccct tcctcctgga gcttgtaggc cctcagcttg ttcaaaaggc
    10501 cgatcccgcg gccttcctgc gagaggtaga gaagcacgcc gcggccttcg tcctcgatca
    10561 tcgccatcgc cgccgcgagc tgctcgccgc agtcacagcg catgctgtgg aagacgtcgc
    10621 cggtcaggca ttccgaatgc acccggacca gggcgggctc gccggtgtcg atctcaccct
    10681 tgaccattgc gacgtgatgc ttgtcgtcga ccagggagcg gaaaccgact gcgcggaagt
    10741 caccgaaact ggtcggcagc gcggtcgaga cgacccgttc caccagtttc tcgttctcgc
    10801 gccggtaggc gatcaggtcg gcgatcgtga ccatcttgag gccgtgcttt tcggcgtagc
    10861 cgaccaggtc gtcgacccgg gccatggtgc cgtcctcgtt catcacctcg cagatcacgc
    10921 cggccgggga aacaccggcc aggcgggcga gatcgaccgc ggcctcggtg tggccggttc
    10981 gctccagcac gccaccgtcc ttggctttga gcgggaatac atgtccgggg acgacgatgt
    11041 cccgcggagt cgcgtcgggg tcgatcgcga cctggaccgt gtgggcccgg tcgtgggcgg
    11101 agatgccggt tgtcacgcct tcggccgctt cgatcgaaac ggtgaaggcg gtttccagcg
    11161 gcgcctcgtt cttggcggtc atcaggcgca ggccgagctg gtcgcagcgc tgggcggtga
    11221 gtgaaaggca gatcagcccc cgcccttcct tggccatgaa gttgatcgct tcgggagtcg
    11281 cgaactgggc ggccatcacg aggtcgcctt cgttttcgcg gtcctcgtca tcggtgacga
    11341 tgatcatccg gccggccctg atctcctcca gggcctcttc gatcgtggcg aacggagtcg
    11401 cttcagtggt actcacgctg ccgcgccctt cttcggtagc catgctttgt tcagactttc
    11461 cttttggttt ctcaacggtc cgtgtggctt tcggaccctg gtgcatatgg tgacaccatg
    11521 cgctcgacgt atttggcgat tacgtcacac tcgatgttga cggttgtgcc gatctgaagc
    11581 gttccgaggt tggtgcgctc ccgggtttcg gggatcagcg ccacctcggc ccagtcctcg
    11641 ccgcaatcgg cgacggtgag gctgacgccc tcgatcgtga tcgaaccctg cgggatcacg
    11701 taacgcgaaa gttccgccgg cagtgaaatc ctgagtcgca gcgagaagcc gtcctgctcg
    11761 cggccgatca cgcccgcggt gccgtcgacg tgaccctgga cgatgtggcc gccgagccgg
    11821 tcgctcgcct tgagggccag ttcgagattc accggtgaac cggcttcgag cccgccgatc
    11881 gaagtcaggt caagcgtctg ctgcatcgcg tcggcggtga agccgcccgc atcgaccgcg
    11941 gtcgcggtca ggcagcagcc gttgacggcg accgaatcgc cgaggccgat ctccccggcc
    12001 agcgtggtgg cgacgctgat ccgggcccct tcggatgaaa ggtcgatgga cttgacctca
    12061 ccgaggtcct gcaccagtcc ggagaacatc accaggtcct gatcgtcgaa gacatgagca
    12121 catcttgccc gacccggctc acgcgcatct tcggcactcg ggtcgcacct tccatcagtt
    12181 ccggtccgga accctcggta gccgggcgtc cgccgccgag aatcaggggc gcgatgaaga
    12241 tctcggtccg gtcgacttca ccggcggtga gtgccgcccc ggcgagacgc gggccacctt
    12301 ccagcaggag cgaaccgatc cctcgctcgc ccagcgcttc catcgccgcg gcaaaccggg
    12361 ccccggaacc ttcacccacc accgggatca cttcggctcc ggccgtttcc aacgcggcca
    12421 gccggtcggg gtccggatcg ggaccggcaa tgatcaagag cggcgcctcg tcgatatcgg
    12481 cgaagagggc tgccccggtc gtgatgagca gcccagagtc aaagatcacc ctcgccggct
    12541 gccggaccgg ttcgccgtcg gtgtggcgcg cggtcagtct cgggtcgtcg gcggtcaagg
    12601 tgccggagcc gaccgccacc gcgtccatct cggcccgcca gcggtgaacg agtgcccggc
    12661 tctcttcgcc ggaaatccat tttgagtccc cggtccgggt cgcgaccttg ccgtcgaggc
    12721 tcatcgccat cttcaaggtg accaggggtt tgccggtcag ggcccgcttg cgaaagtcct
    12781 gaatcagggc gagcgccgcg tcggcagccg gcccttcggc ccagcggacc cggatcccgg
    12841 cgtcttcgag ccgggccggg ccaacgcccc gggtcttttc ggtcgggtcc tcggcggcga
    12901 tcaccacctc gcctaccccg gcctcaatca gcccgtcggc gcagggcggc tggctgccgg
    12961 tgtgggcgca gggttccagg gtcacgaaga tcgtggcgcc ggccgggtcg ttgccgcgcc
    13021 tttccgcatc ggccaatgcc tcccgttcgg cgtggagctc cccgtggcgg cgatgccagc
    13081 cttcaccgat cacccggccg tccttgacca gcaccgcacc gaccgccgga ttggggctgg
    13141 tccagccgag gccgtgttcg gccagttgga tggccctttg ccagtagtcg gcctcagagg
    13201 tgggttgagc ggcagtcatg ggagaatcct cagcctaatg aaggtaatca tccagatccc
    13261 ctgcctcaac gaggaggaga ccctgcctgc aactctcgcg gacctgccgc gcgagatcga
    13321 aggcgtggat gaaatcgaga ccttggtcat cgatgacggc tcaaccgacc ggacgatcga
    13381 agtcgcccgg caatgcggcg tcgaccacat cgtcaagctg cccaacaacc gcggtctggc
    13441 aaccggtttc cagtccggac ttgatgcctg cctcaagctc ggggccgaca tcgtcgtcaa
    13501 caccgacgcc gacaaccagt actccggaag cgacattccg aagctggtcg cgccgatcct
    13561 ggccggtgat gccgagatgg tggtcgggga ccgcaaagtg gccgagatcg atcacttctc
    13621 accggccaag aaggttctcc agaagttcgg cagctgggtc gtgcgccagc tttccaacac
    13681 cgaggtcacc gatacgactt ccggttttcg cgcctacaac cgggaagcgg cacttcagct
    13741 gctggtcgtc gacaacttca cctacacgct cgaaagcctg atccaggccg gcaaggggaa
    13801 cgtcgcggtc ggcgaggtca cgatctcgac caacgagaag acccgcgagt cccgcctgtt
    13861 cggttcgact tccgcctatg tgcgccgcaa cggcctggcg atcctccgga tctacacccg
    13921 ctacgagccg ctcaaggtct tttcgctgct cggcttgatc gccgccgtgg ccgccatcgc
    13981 agcctggatg ccgttcctga tcgactggat cgtgaacggc gaccgcgacg gtcacatcca
    14041 gtcgctgctg ctcggtgcgg tgctcttcat cgtctcgatc cagctcttcg cgctcggcat
    14101 catcggtgat ctgctggccg gccagcgggt catgacccag cgcgtctatg aaagggtccg
    14161 ccggatcgag cttgagctcg aagtcgagcc gagccatcga ctcgatcccc ctcgtgaccg
    14221 aagcgccgtc cagtaatcac ttgagcgatt ccccggagaa aaccgaatct tcgacccgtg
    14281 cctacggccg gtccgtcggg ctgctcacga tcggggtcgg cctcaccggc atcttcacct
    14341 acgcctactt cctgatcgct tcccatgacc tgagcaagga cgcctacggc gagatcaccg
    14401 tgctctggtc cgcggtcttc atcaccgtgt cgactctcta ccgcccggtt gaccagctgc
    14461 tctcccggca catctcggaa cacatcgaac ggggcgagac cgacgtcggt ccggtccggg
    14521 tcgcggcgaa gatccagatc tgcctggcga tcggcttcgc gatcaccgcc ctgatcctga
    14581 agaaaccgct cgaggacggg ctgctcgccg gcaacgaaac gctctactgg gtcttcttct
    14641 cctcggtcct cttctatgcc gccagctact tcgcccgggg ctatctggcc ggccaccagc
    14701 agttcggact cttcacgacc ctgatcctct ccgagtcggt cttccgcacg ctattcgccc
    14761 tgctggtcgc cttcgggctg ctttccggcc agtcctgggt cgcgatgggc atcgtcgccg
    14821 cccccctgct cagcctgatg gtagtgcctt cggcgatcac gcggcgcagc cggcgcgagg
    14881 ccgaggagcg ggcccgggtt gccgagcagc gcggcacagc gccagaggac ttcgacgccg
    14941 aggtcgccga ggcccgggag gagttctcgc tttcacacgg cgccggcttc gtcggctcgg
    15001 tcttcctgat catgttcagc gagcaggtct ttctcaacgc cggcccgctg gtggtcaggg
    15061 cggtcgaagg agtggccgcg gccggctaca tcttcaacgt cctgatgatc gcccgggccc
    15121 cgctgcagct ctttcaggcc gtttcgactt cgatcctccc ccacctgacc gcgctgcacc
    15181 acagctcgga tccgaactcg gagaaggagt tccaccactc ggtccggatg gtgctgctcg
    15241 gaatcgccgc cttcaccgcg ctgaccgcgg tcgtggtgat gatcgccggc ccgaagctga
    15301 tgcagatcgc cttctcggac aagttcacct atgaccggct gggcctgatg ctggtcaccg
    15361 ccggcatggg cctgtacctg gccgcggtca cggtcaacca ggcctgcctc gcccagggcc
    15421 aggcccggcg ttcctcgatc cgctggatct cctgtgcggt cttcttcatc atctggacga
    15481 tcctcccgtt catcagcgat ggcaacctga gggtcgagat cggcttcgcc ctgaccgccc
    15541 tgctcctgct cagcctgctg accttcatct acttccggcc gaagggcgtc gaagcggacg
    15601 tgcctctcga aggctcatcc gaggcgatcg aggccagact cgccggcctc gaagacactt
    15661 cgacctaggt cgcctcaatc agtcttgaag ttattcgctc catgtgcgaa taacttcaac
    15721 gctgatctga gcggtcgagc cccgaaggac tcaggcaccg gattccgcgg cagcgtgaat
    15781 gtcgcggtaa gcctgagccg ggtcgtcgga accgaagatc gccgagccgg cgaccatcag
    15841 ggtcgcgccg gccgcggcca tcgttccggc ggtcccggtg ccgatgccgc cgtcaacctc
    15901 gatcaggacg tcatcaccga ccagcgagcg cattcgcctg accttgtccg gcgaggtttc
    15961 gatgaagggc tggccgcccc agccggggtt cacggtcatg accaggatct cgtcgaccat
    16021 tcccttcagc tcggaaaccg tttcgaccgg agtgccgggg ttgatcgcga cgccggccag
    16081 acagcccaac tcgcgaatcg cgtggcagca gcggtgggca tgcggggtcg cttcctcatg
    16141 gaagatgatc gaatccgccc cggcccgggc gaagtcctcg aactgacggt cgggatcctc
    16201 gatcatcagg tggacgtcaa ccaccccgcc ggcgtcatgg acctgatcgg cgatgccgga
    16261 aagcaccagc gggccgatcg tgatcggcgg gacgaactgg ccgtccatca cgtcgaagtg
    16321 aatgaccctg gcgccggcgt ccatgatctc ggcgatctgc gagcccagcc ggccgaagtc
    16381 ggcggagagg atcgaaggag cgatgcgg
//
LOCUS       DAOQKN010000034        16123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_1658_length_16123_cov_5.845656, whole genome shotgun sequence.
ACCESSION   DAOQKN010000034 DAOQKN010000000
VERSION     DAOQKN010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 16123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16123
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1658_length_16123_cov_5.845656"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1714
                     /locus_tag="PLJ64_03840"
     CDS             <1..1714
                     /locus_tag="PLJ64_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC92654.1"
                     /translation="EGAVDAANLLKPMLARGELRAVGATTLDEYRQHIEKDAALERRF
                     QPVLVGEPDVPDTIAILRGLKERYEVHHGVRIADSAIIAAATLSERYIADRFLPDKAI
                     DLIDESASRLKIEIDSMPTEIDEVERRIQQLEIEREALKAETDEASAGRRDTLEEELA
                     ELNEKSSAMKARWQTEKGLIESIKEAKARHEEASREAERAERDADLERAARLRYSEIP
                     ELEKQVADAEARLTELQAEGGTMLTEEVTEQDVAEVVAKWTGIPVTRLMEGEVEKLVH
                     MEERLHDRVIGQDEAVDAVANALRRSRAGLSDPDRPIGSFLFLGPTGVGKTELAKSLA
                     EFMFDTDQAIVRLDMSEYMEKHTVARLIGAPPGYVGYEEGGQLTEAVRRRPYAVVLLD
                     EIEKAHPDVFNTLLQLMDDGRLTDGQGRTVSFTNTVLIMTSNVGSQEIAGEDVDERIR
                     ERVTEVLETTFKPEFLNRIDDTVIFHRLSKEDIGKIVEIQVEGLTRRVAERDIEIELT
                     DDARTLLGNLGYDPTYGARPLKRVVQKQLVDKLALKILEGEFTEGDSVLIDAAEGELA
                     FSKK"
     gene            1919..2758
                     /locus_tag="PLJ64_03845"
     CDS             1919..2758
                     /locus_tag="PLJ64_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92655.1"
                     /translation="MARAQNNIQQAKPAAPGGPLWLLRRFGWFIEKYLLWPIADSFRA
                     IGRGIARIPNAFRYRSPMAYIGVTLMLTLTAGAIAAAVYFYKQQEDKGQDPAVATTAV
                     PTETVVPSVPTPTTTPTTVEDTTPNSGDVDQTLQGVVPNFQTSGKTKQSGAGADSPAG
                     KLPGTVVRPSPVPDSPPLKVAHRFATIFANYEVGGKGAAKQFSSVATAKLARELRENP
                     PRLPSNGQIPKATVVNVVAGKRSGDSLDASVSLMRSGAASELRLDLVREKGKGWLVDE
                     VRG"
     gene            2768..4513
                     /locus_tag="PLJ64_03850"
     CDS             2768..4513
                     /locus_tag="PLJ64_03850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HNC92656.1"
                     /translation="MGRFRTALATISVGALLATGHAASAESATPITDEPATTAGIGPI
                     GPTGTSGSTGSTGNTGNTGNTGNTGNTGSTGNPGNNGNSGNGNTGGVDPDGNTGGNQN
                     QGSGNNGNQTPNPQTENPAKAEEIIPNSDSPLSATITTSPGLGLSGDSTPVAACDGSA
                     GPPRNLVPIYVEAAKKYGLGEQGPQILAAINSIETDFGRLNDVTSYAGAIGWMQFMPS
                     TWDMYATDGDGDGKTDPYNPHDAIHAAARYLQAAGAPGDWYDAIYAYNHADWYVQDVL
                     DKAACYGALGDTIDAEEEHRNLFCEPSEARALEIPNYYMRGFEDAASRYDLGKRGVWV
                     LAAVARLESDYGRGMTAKQLKDTGPMGLSRDEWKQYGVDGDGDGYVERDEPWDGIATF
                     ARMLWSQPTLEAGLFEHNHATWYVEAAVQDAGNIAGDCQTVKTDWNISYPITTNATEI
                     NWDNLQVLNPAAQEDIQNGLIDQRVINLLAILTQKYSLLISALRSDHSQMTASGNVSN
                     HYYGRAMDIAAVNGVPCTDQSADSPCTEAIMLLASLPDGVKPTELIYGWDVDGSGPAF
                     ALPDHTNHIHAGFGP"
     gene            4575..6044
                     /locus_tag="PLJ64_03855"
     CDS             4575..6044
                     /locus_tag="PLJ64_03855"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin monoamine oxidase family protein"
                     /protein_id="HNC92657.1"
                     /translation="MSEKISRRRLVQAGAVAAAGAALPAAGAEAKNRRKKKPVQDVVV
                     VGAGLAGLMAARQLAAKGRKVKVLEARGRVGGRTYSPKHGGVTLDVGGQWLGPTQDKV
                     AALADKLGVQTFKTYWEGQAVYQRNGSRTLFNVTSPIPPDPDAAETLNLVLQLESMGA
                     GISRSEPWKDANADLYDGQTFETFKLANTTGEGARFLTDLATNAVWACEPRDISLLHL
                     LFYISSAGNETTPGSFIRLTSTTEGAQDSRFVGGSQQLSIRMAKQLGSRVQLNAPVRQ
                     IRQEKGLCYVTSDKGTWRARQVIVAVPPAVSAFIDYDPILPSDRAQLIQRFPQGNAIK
                     VQAIYDTPFWREEGLSGYVNSDEGPVKLMYDNSPHDGSAGVLLGFIEGQEARLAGRMS
                     PAARKAAVLANFKKTIGNAAGSPKAYVEKNWAEEEWTRGCYGGFLPPGVLTGFGPALR
                     KPHGRIHWAGTEHAEIWNGYMDGALRSGEDAARAALARL"
     gene            complement(6093..7805)
                     /locus_tag="PLJ64_03860"
     CDS             complement(6093..7805)
                     /locus_tag="PLJ64_03860"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNC92658.1"
                     /translation="MSEEIWERKVPPMPEDPDVVVIGSGLAGLTAAAYLAANDQRVLV
                     LEQYDVAGGCSQTFRRKNKWQFDVGLHYIGGVKTGEIARILQGIGLHERIEFPELDPD
                     GFDTFVFPDLEFKVPKGWDNYVGRLVETFPEEEEGLRGVTDVMRNLMDELREVGIPDS
                     NTDLAEYVEKAPTVVSWGMRSVNELFEEHYLSERSRAVLIGQAGDYATPPSRTPVILH
                     AGLVDHYLQEGAFYVRGGGQELAGHLVDLIHSYGGRVRTHAEVEHIATASTEDGGHRV
                     TGVELKGGEVIDTGNVIATGDIKHLFLDLLDSEAVPEGIREQVKAYRMATPLFTVYLG
                     LDFDITERMPATNYWLHSRYDQEAYYDPIAAGEFDTDPPVYISSASAKDPGHEGHAPE
                     GCSTVELMTWATPDPEAWGVDESEDAPKYSRQAAYREAKDRLTEQLIDRAEDQLGEIR
                     SHILWKEAATPMTQTRYTLSTGGSSYGIELATDQFGPLRLEYRTPIEGLVLGGVSSRR
                     GHGIVGAMSGGVESASAVLGRNLRAEVAGGEVFGEPAILTSGGEGWDALEACRKLQVK
                     ALRA"
     gene            7889..8458
                     /locus_tag="PLJ64_03865"
     CDS             7889..8458
                     /locus_tag="PLJ64_03865"
                     /inference="COORDINATES: protein motif:HMM:NF021886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNC92659.1"
                     /translation="MALKHAVLAALSTEPGSGYELSKRFDASVANFWPASAQQIYREL
                     DRLEGEGLVRARTVHQQKRPDKRVFRITPAGTDELSGFIRSATRPTVIRDDLLVKIAS
                     LNRDNAGEVVAAVRERAAASSEKLAMYEHLREALLGAGTEQEFLAAGPDRPDFGGYLA
                     LKRGIAFEKGNLRWSREVLSILGESRSRP"
     gene            8577..8858
                     /locus_tag="PLJ64_03870"
     CDS             8577..8858
                     /locus_tag="PLJ64_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HNC92660.1"
                     /translation="MPTFVMLTNLTSEGVKTLKSHPGRVAEVNREVEALGARVVAQYA
                     TLGQYDFVTIIEAESAEVMARVSVEMGSRGTMSSQTLSALPAEQLAEVL"
     gene            8855..10147
                     /gene="purD"
                     /locus_tag="PLJ64_03875"
     CDS             8855..10147
                     /gene="purD"
                     /locus_tag="PLJ64_03875"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNC92661.1"
                     /translation="MSVRILVLGGGGREHAIVKALARSPQSPELFAAPGNPGIAADAT
                     CLPDLDPADPAAVTAKARELEIDLVAVGPEAPLVAGVVDALSDAGIAAFGPSGAAAQL
                     EGSKAYAKEAMEEAGVPTAGYGVVSDRESALAKVAETGCPVVLKADGLAAGKGVLICL
                     TEEEAREAIEVFFTERRFGKTGVVIEEFLEGEELSLLAICDGERAIPLAPAQDYKRIG
                     EGDTGPNTGGMGSYSPVQTVSKDQLDELSALVHQPIVDLMARRGHPFHGVLYAGLMMT
                     ADGPKVLEYNTRFGDPETQAVLPRLESDLVDLFLSASEPGGLAGTEVSFADQFAVTVV
                     LASAGYPASSSKGDVITGLDEAARSAEVVHAGTAEADGRIVTAGGRVLNLTGTGPDAA
                     TARDRAYDAADAVNFDGKQMRRDIAERAVRRERNQKAT"
     gene            10144..10617
                     /gene="purE"
                     /locus_tag="PLJ64_03880"
     CDS             10144..10617
                     /gene="purE"
                     /locus_tag="PLJ64_03880"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNC92662.1"
                     /translation="MSEETQQPATLSSVEELFEEIDVEQPQVGIIMGSKNDKPKMQPA
                     GQALEDAGIAYEVRVMSAHRDPVLVAEYCTNAKMRGLKVIIAGAGMSAALPGVAAAHT
                     ELPVIGVPILGKSLGGLDALLSVAQMPPGVPVACVAVDGAKNAGVLATRIINLAG"
     gene            10624..11907
                     /gene="purB"
                     /locus_tag="PLJ64_03885"
     CDS             10624..11907
                     /gene="purB"
                     /locus_tag="PLJ64_03885"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNC92663.1"
                     /translation="MIERYTRPGMGAVWTQQAKFQSWLDVELAATDAWAAEGVVPPEA
                     AAACRERASFTVEEINAREEITNHDVAAFVDVVAASIGEEGRWIHFGLTSSDVLDTAL
                     AVQLTKAGEILVEGAKKYRDALIDRAFEFKDTLAMGRTHGVHAEPTTFGLRLAGFAFE
                     ADRNYRRLQRATEQAARGKLSGAVGTYASVPPAIERRVMDALGLTAEEIATQVVPRDR
                     HAELLSAIALAGAGIERFATEVRHLQRTEVREAEEPFATGQQKGSSAMPHKRNPIRTE
                     RMTGLARLLRGYAQTGLENVALWHERDISHSGAERVILPDATIGLDYMHHVITQVASG
                     LVVHVDRLQANLEQTNGAMFSQRALTALVESGMTRDDAYRTVQAAAHEAFDGDTHFRE
                     LIGKAAPELDLDEIFDYNAYLTHLPEVFERLEEIR"
     gene            11969..12259
                     /locus_tag="PLJ64_03890"
     CDS             11969..12259
                     /locus_tag="PLJ64_03890"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase domain-containing
                     protein"
                     /protein_id="HNC92664.1"
                     /translation="MKLYTCGTGTSGPAGVHPCAKAGKALDDAGYEYELRKVRGYRML
                     PWTWKSRAEDRKEVKELSGTNEVPILVLDDGEVISDSSAIAKWAKENPAPGR"
     gene            12360..12884
                     /locus_tag="PLJ64_03895"
     CDS             12360..12884
                     /locus_tag="PLJ64_03895"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC92665.1"
                     /translation="MWLLPDDRVRARVERRQYRSVVRHIFVSRRSGWISLREDGEAAG
                     AVLWYPPGTKKHSFRESLAESIPFLPAGLPRLGKATRLQKLVVSNWPKEPHWYLSVLS
                     VSPESQRQGHGGALLGPGLARADRQGVGCWLETQRESNIPFYARFGFELVKKLEIEAG
                     VNLWLMWRPPLEGD"
     gene            complement(12926..13855)
                     /locus_tag="PLJ64_03900"
     CDS             complement(12926..13855)
                     /locus_tag="PLJ64_03900"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HNC92666.1"
                     /translation="MRTFLVASVSLIASAFALSGVAAAAEVPPCTGSFSQKTIYSGQG
                     SIEALIVGGGGTLFASGGDTTQSTLRAYTKGKADPETVATGNPGGGGLAWNGKRLLWG
                     YGDLLANGLTGDLDPTAGLYSVNVAAKKKSLVSDTLGMANGVARGKDGYIYASNNLGL
                     KLDRITPDGDTENGWASLNSANGLTVGRNGRYLYANQMFETPSTIAKIDTRDPSKVFT
                     YFTSPEAGNVLLDGLAHDSGNNLYTAAYGKGEVWKITPDRQACVIATGLSQTTAVAMS
                     SAGKGFRAGSLYAAGFDGNIVEVTGAAKAAVPG"
     gene            13915..14766
                     /locus_tag="PLJ64_03905"
     CDS             13915..14766
                     /locus_tag="PLJ64_03905"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNC92667.1"
                     /translation="MANSIEDLKLVNSGKVREIYEIEDDLLLVASDRISIYDVIMPTA
                     IPDKGKVLTQMSNFWFENTTDIVPNHFLSNDVPEEVAGRAVRVRKLEMYPVECVVRGY
                     LSGSGWKEYQETGAVCGVGLPDGLQESDKLPRPIFTPATKAELGDHDENVDFDRAAEI
                     VGDRHLMEELRRISIEIYEYGANHAAEKGIILADTKFEFGSYAGAEIVLGDEVLTPDS
                     SRYWPADQYEPGKSQPSFDKQYVRDWANAAGWDHNPPAPELPEDVVAQTQAKYREAYE
                     RITGREL"
     gene            14763..14993
                     /gene="purS"
                     /locus_tag="PLJ64_03910"
     CDS             14763..14993
                     /gene="purS"
                     /locus_tag="PLJ64_03910"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HNC92668.1"
                     /translation="MIQARVLIRPKEGILDAQGKAVEGALPALGFSGIEHVRIGRIVE
                     LEADSTDQLEELCEKLLANPLIEDYEIETVSG"
     gene            14990..15697
                     /gene="purQ"
                     /locus_tag="PLJ64_03915"
     CDS             14990..15697
                     /gene="purQ"
                     /locus_tag="PLJ64_03915"
                     /inference="COORDINATES: protein motif:HMM:NF024897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HNC92669.1"
                     /translation="MKWGVLQFPGSCDERDALNACQIAATALGSEGGSGSLPRLVWHE
                     ETDLSDVDGIVVPGGFSYGDYLRAGAIARFSPAMTAVAEFAAAGGPVLGICNGFQVLC
                     ESGLLPGALLTNEKTRFICRQGELIVGEAESPFLSGYEAGQRISIPFKHQSGRFFAPA
                     DLLEEIESNNQVAFRYAPGQNPNGSLNDIAGVTNEAGNVIGLMPHPEHAVEAFAGSTD
                     GLALFQGPAIAATGAVA"
     gene            15694..>16123
                     /locus_tag="PLJ64_03920"
     CDS             15694..>16123
                     /locus_tag="PLJ64_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HNC92670.1"
                     /translation="MSEQSAQREDKGEQSAHRALGLTDREFDLICEKMGREPNGVELA
                     MFSLLWSEHCAYKHSRKLLKKLPTEGPRVVMGPGENAGAVDIGNGFSVAFKVESHNHP
                     SAVEPFQGAATGVGGILRDVIALGARPIAILDSLRFGEPDT"
BASE COUNT         2900 a         5358 c         5311 g         2554 t
ORIGIN      
        1 cgaaggcgcg gtcgatgccg ccaacctgct caagccgatg ctggcccgcg gcgaactccg
       61 cgcggtgggt gccacgaccc tcgacgagta ccgccagcac atcgagaagg acgctgccct
      121 cgaacggcgc ttccagccgg tcctggtcgg cgagcccgac gtgccggaca cgatcgcgat
      181 cctgcgcggg ctcaaggagc gctacgaggt ccatcacggc gtccggattg ccgactcggc
      241 gatcatcgcc gccgccaccc tttccgagcg ctacatcgca gaccgcttcc tgccggacaa
      301 ggcgatcgac ctgatcgacg agtcggcatc ccggctgaag atcgagatcg actcgatgcc
      361 gaccgaaatc gacgaagtcg agcggcgaat ccagcagctc gaaatcgaac gcgaggcact
      421 caaggccgag accgacgagg cctcggccgg ccgccgcgac accctcgagg aagagctggc
      481 cgagctgaac gagaaatctt ccgcgatgaa ggcccgctgg cagaccgaga agggcctgat
      541 cgaatcgatc aaggaggcaa aggcccggca tgaggaggca agccgcgagg ccgaacgggc
      601 cgagcgcgat gccgacctcg aacgggctgc ccgcctgcgg tactcggaga ttcccgagct
      661 cgaaaagcag gtcgccgacg ccgaggcgag attgaccgag ctgcaggcag agggcggcac
      721 gatgctgacc gaggaggtca ccgaacagga cgtggccgag gtggtcgcca agtggaccgg
      781 gatcccggtc acccgcctga tggagggcga ggtcgagaag ctggtccaca tggaggagcg
      841 cctccacgac cgggtgatcg gccaggacga ggccgttgat gcggtcgcca acgcgctgcg
      901 ccgctcgcgg gccgggcttt cggacccgga ccgcccgatc ggctccttcc tcttcctcgg
      961 gccgaccgga gtcggcaaga ccgagctggc caagtcactg gccgagttca tgttcgacac
     1021 cgaccaggcg atcgtcaggc tcgacatgtc cgagtacatg gagaagcaca ccgtcgcccg
     1081 gctgatcggc gcacctcccg gatacgtcgg atacgaagaa ggcggccagc tgaccgaggc
     1141 ggtccggcgc cggccctacg cggtggtcct gctcgacgag atcgagaagg ctcatccgga
     1201 cgtcttcaac acgctgctcc agctgatgga cgacggccgc ctgaccgacg gccagggccg
     1261 gacagtcagc ttcaccaaca cggtgctgat catgacctcg aacgtcggct cgcaggagat
     1321 tgccggcgag gacgtcgacg aacggatccg cgagcgggtg accgaggttc tcgaaacgac
     1381 cttcaagccc gagttcctca accggatcga tgacacggtg atcttccacc gcctctcgaa
     1441 ggaggacatt ggaaagatcg tcgaaatcca ggtggaaggc ctgacccgcc gggtggcgga
     1501 gcgggacatc gagatcgagc tcacggacga tgccaggacc ctgctcggca acctcgggta
     1561 cgacccgacc tacggtgccc ggccgctcaa gcgggtggtc cagaagcagc tggtggacaa
     1621 gctcgccctg aagatcctcg aaggggagtt caccgagggt gattcggtcc tgatcgatgc
     1681 cgccgagggc gaactcgcct tctcaaagaa atagggcttt cttgatcaag atgatatgaa
     1741 atgtgggtgg agagtcgaaa tcggctctcc atctatgttt catgcgagac acgggttgca
     1801 ctttcgcacc cgcttaaccg cttctttact tcctcttttg ggttcccgta ttaccctgca
     1861 cgctccgcgt ctggcctcag ggaggcgtat cggaggcgga aagcaggccc cggacccgat
     1921 ggcgagagct caaaacaaca tccagcaggc gaagccagcc gctcccggcg ggccgctttg
     1981 gctgctccgg cgcttcggct ggtttatcga gaagtacctt ctctggccga tcgcggactc
     2041 cttccgtgcg atcggccgag ggattgcccg gatccccaat gctttccgct accgcagccc
     2101 gatggcctac atcggcgtca ccctgatgct caccttgacc gccggtgcga tcgctgccgc
     2161 cgtctacttc tacaagcagc aggaggacaa gggccaggat cccgccgtcg ccaccaccgc
     2221 ggtgccgacc gagacggtcg ttccctcggt tccgacgccg accaccaccc cgaccacggt
     2281 cgaagacaca accccgaact cgggtgatgt cgaccagacc cttcagggcg tggtcccgaa
     2341 cttccagact tctggcaaga cgaaacaaag cggtgccggc gcagatagcc cggccgggaa
     2401 gcttccgggg accgtggtcc ggccgtcccc cgtccccgac tccccgccgc tcaaggtcgc
     2461 ccacaggttc gccacgatct tcgccaacta cgaggttggc ggcaagggtg cggccaaaca
     2521 gttttcttcc gtcgccaccg cgaagctggc ccgcgaactg cgggagaacc cgccgcggtt
     2581 gccgtcaaac ggccagatcc ccaaggcgac agtggtcaac gtggtcgccg gcaagaggtc
     2641 cggcgactcg cttgacgcga gcgtctctct gatgcgctcc ggtgcggcct ccgaactgcg
     2701 gctcgacctg gtccgggaga agggcaaggg ctggctggtg gacgaggtac ggggataggc
     2761 ggaaagagtg gggaggttca gaacagctct ggcgacgatc tcggtcggcg ccctgctcgc
     2821 caccggccac gccgccagtg cagaaagcgc gacgccgatt accgacgagc ccgccaccac
     2881 cgccggcatc ggtccgatcg ggccgaccgg caccagcgga tcgaccggtt ccaccggcaa
     2941 tacaggcaat accgggaaca ccggcaatac ggggaacacc ggcagcaccg ggaatccggg
     3001 caacaacggt aactccggca acggcaacac cgggggcgtc gatcccgacg gcaacaccgg
     3061 cggcaaccag aatcagggct ccggcaacaa cggcaaccag acgccgaacc cccagacgga
     3121 aaacccggcg aaggctgaag agatcatccc gaacagcgac tcgccgcttt cggcgaccat
     3181 cacgaccagc cccggcctcg gcctctctgg tgattccacc ccggtcgccg cctgcgacgg
     3241 cagcgccggc ccgccccgca acctggtccc gatctacgtc gaggccgcca agaagtacgg
     3301 tctcggcgag cagggcccgc agatcctcgc cgcgatcaat tcgatcgaga ccgacttcgg
     3361 ccgcctcaac gacgtgactt cctacgccgg ggcgatcggc tggatgcagt tcatgccctc
     3421 gacctgggac atgtacgcga ccgacggtga cggcgacggc aagaccgacc cctacaaccc
     3481 gcatgatgcg atccatgccg ccgcccgcta cctgcaggcc gcgggcgcgc cgggcgactg
     3541 gtacgacgcg atttacgcct acaaccacgc cgactggtac gtccaggatg tgctcgacaa
     3601 ggccgcctgt tacggcgcac tcggagacac gatcgatgcc gaagaggagc acaggaacct
     3661 gttctgcgag ccctccgagg cgcgggccct ggaaatcccg aactactaca tgcgtggctt
     3721 cgaggacgcg gcctcgaggt acgacctcgg caagcgcggc gtctgggtgc tggccgcagt
     3781 ggcccggctc gaatccgact atggccgcgg catgaccgcg aagcagttga aggacaccgg
     3841 tccgatgggt ctttcccgcg acgagtggaa gcagtacggg gtcgacggtg acggtgacgg
     3901 gtacgtcgag cgggacgagc cctgggacgg gatcgcgacc ttcgcccgga tgctctggtc
     3961 acagccgacg ctcgaggccg gactcttcga acacaaccac gccacctggt acgtcgaggc
     4021 ggcggtccag gatgccggca acatcgccgg cgactgccag accgtcaaga ccgactggaa
     4081 catctcctat ccgatcacca ccaacgcgac cgagatcaac tgggacaacc tgcaggtcct
     4141 caacccggct gcccaggaag acatccagaa cggcctgatc gaccagcggg ttatcaacct
     4201 cctcgcgatc ctgacccaga agtacagcct gctgatcagc gccctgcgct ccgatcacag
     4261 ccagatgacc gcctccggca acgtctcgaa ccactattac ggacgggcca tggacatcgc
     4321 cgcggtaaac ggcgtcccct gcaccgacca gtcggccgac agcccctgca ccgaggcgat
     4381 catgctgctg gcctccctgc ccgacggggt caagccgacc gagctgatct acggctggga
     4441 tgtggacgga tccggaccgg ccttcgcgct cccggaccac accaaccaca tccacgccgg
     4501 cttcggcccc tgagttcact tcccctcgat ttctttggcg taggatcttc tccagtccaa
     4561 ccagggaggt tcctttgagt gagaagattt ctcgtcggcg gctggttcag gccggggccg
     4621 tcgcggcggc cggggcggca ctgccggcgg ctggtgccga agcgaagaac cggaggaaga
     4681 agaagccggt tcaggacgtg gtcgtggtcg gggccggact ggccggattg atggccgcgc
     4741 gtcaactcgc cgccaaaggc aggaaggtca aggtcctcga ggcccggggc cgggtcggcg
     4801 ggaggaccta ctcgccgaag cacggcggcg tgacgcttga cgtcggcggc cagtggctcg
     4861 ggccaaccca ggacaaggtc gccgccctag ccgacaagct cggcgtgcag accttcaaga
     4921 cctactggga gggccaggcc gtctaccagc gaaacggcag ccggacactg ttcaacgtga
     4981 cctcgccgat cccgcccgac ccggatgcgg ccgagaccct caacctcgta ctccagctgg
     5041 agagcatggg ggcaggaatc tcgcgttccg aaccgtggaa ggacgcaaac gccgacctgt
     5101 atgacggcca gaccttcgag actttcaaac tcgccaacac caccggcgag ggcgcgcgct
     5161 ttctgaccga cctcgccacc aatgcggtct gggcctgcga gccgcgggac atctccctgc
     5221 tccacctcct cttctacatc tcctcggccg gcaacgagac caccccgggc agtttcatca
     5281 ggctgacctc gaccaccgag ggcgcccagg attcgcgatt cgtgggcggc tcgcagcagc
     5341 tttcgatccg gatggcgaaa cagctcggct cccgggttca gctcaacgcg ccggtccggc
     5401 agatccgcca ggagaagggc ctttgttacg tgaccagcga caagggcacc tggcgggcca
     5461 ggcaggtgat cgtggcagtg ccgccggcgg tcagtgcctt catcgattat gacccgatcc
     5521 tcccttccga ccgggcccag ctgatccagc gcttcccgca gggcaacgcg atcaaggtcc
     5581 aggcgatcta cgacaccccg ttctggcggg aggaagggct ctccggatac gtcaactcgg
     5641 atgagggccc ggtcaagctg atgtacgaca actcccccca cgacggctcg gccggcgtgt
     5701 tgctcgggtt catcgagggc caggaagcga ggctggccgg acggatgagc ccggccgcgc
     5761 gcaaggccgc cgtgctcgcc aatttcaaga agacgatcgg caacgcggcc ggttcaccca
     5821 aggcctacgt cgagaagaac tgggccgagg aagagtggac ccgcggctgc tacggcggct
     5881 ttttgccccc gggtgtcctg accggtttcg gaccggcgct gcgcaagcct cacggtcgca
     5941 tccactgggc cgggaccgag cacgccgaga tctggaacgg ctacatggac ggcgccctcc
     6001 gttccggcga agacgctgcc agggcggccc tggccagact ctgaaacgca cctccggcga
     6061 ggagcgccgg ggaaggagcg aggagcgccc ggtcaggcgc ggagggcctt gacctgaagc
     6121 ttgcggcagg cttcgagggc gtcccagcct tcgcctcccg aagtgaggat ggccggctcg
     6181 ccgaagacct cgccgccggc gacctcggcc cggaggttcc ggccgaggac ggccgaggcg
     6241 gattccaccc cgccgctcat cgccccgacg atgccgtgac cgcggcggct cgagactccg
     6301 ccaaggacca ggccctcgat cggtgttctg tactcgaggc ggagcggacc gaactggtcg
     6361 gtggccagtt cgatcccgta ggaggaacca ccggtggaaa gcgtgtacct ggtctgggtc
     6421 atcggcgtcg ccgcttcctt ccagaggatg tgcgaacgga tctcgccgag ctgatcctcg
     6481 gcccggtcga tcagctgctc ggtcaggcgg tctttcgcct cgcggtaggc ggcttgccgg
     6541 ctgtacttcg gggcatcttc gctctcgtcg actccccagg cttcggggtc gggggtggcc
     6601 caggtcatca gctcgacggt tgaacatcct tcgggcgcat gaccttcatg acccggatcc
     6661 ttggcgctgg ccgaggagat gtaaaccggg gggtcggtgt caaactcgcc ggccgcgatc
     6721 gggtcgtagt aagcctcctg gtcatagcgg ctgtgcagcc agtaattggt ggctggcatc
     6781 ctctccgtga tgtcgaagtc gagccccaga tagaccgtga agaggggggt cgccatgcga
     6841 taggccttga cctgttcgcg gatcccttcc ggcacggcct cggagtcgag caggtcgagg
     6901 aagaggtgct tgatgtctcc ggtcgcgatc acgttcccgg tatcgatcac ctcgccgcct
     6961 ttcagctcga ccccggtcac gcggtggccg ccgtcttcgg tcgaagcggt ggcgatgtgc
     7021 tcgacttcgg cgtgagtgcg aacccttccc ccgtagctgt gaatgagatc gacgaggtga
     7081 ccggccagct cctgcccgcc gccccggacg tagaaggcac cctcctgcag gtaatggtcg
     7141 accagcccgg cgtggaggat gaccggagtg cgtgagggag gagtcgcgta gtcaccggcc
     7201 tggccgatca acacggcgcg agatcgctcg ctgaggtaat gctcctcgaa gagttcattc
     7261 accgagcgca tgccccagct gaccacggtc ggggccttct cgacgtattc cgccaggtcg
     7321 gtgttgctgt cggggatgcc gacctcacgc agttcgtcca tcaggttccg cataacgtcg
     7381 gtgactcccc gcagaccctc ctcttcctcg ggaaaggtct cgaccagccg gccgacgtag
     7441 ttgtcccagc ccttcggcac cttgaactcg aggtccggga agacgaaggt gtcgaacccg
     7501 tcggggtcaa gttccgggaa ttcgatccgt tcatgcagcc cgatgccctg caggatgcgg
     7561 gcgatctcac cggtcttcac cccgccgatg taatggaggc cgacgtcaaa ctgccacttg
     7621 ttcttgcgac ggaaggtctg gctgcagccc ccggccacgt cgtactgctc gagcacgagc
     7681 acccgttggt cgttggccgc gagataggcc gcagcggtca ggccggccag gcccgaaccg
     7741 atgaccacga catcgggatc ctccggcatc ggcggcacct tgcgctccca gatctcttca
     7801 ctcattccgt cgtcgacctt tctatactca aagatgtgac tactcaattt gttgagtata
     7861 gggcgatacc cgatgggagt aaagcccggt ggctctgaag catgcggtcc tggccgcact
     7921 cagcaccgag cccggttccg gatacgagct ctcgaagcgc ttcgacgcct cggtcgcgaa
     7981 cttctggccg gcctcggccc agcagatcta ccgcgagctg gatcgcctgg agggcgaggg
     8041 gctggttcgc gcccggaccg tccaccagca gaagcggccc gacaagcggg tcttccgcat
     8101 caccccggcc ggaaccgacg agctttccgg cttcatccgc tcggcgaccc gcccgaccgt
     8161 gatccgggat gacctgctgg tcaaaatcgc gagcctgaac cgggacaacg ccggggaggt
     8221 ggtcgccgcc gtccgggaga gggcagccgc gagcagcgag aaactcgcga tgtacgaaca
     8281 cctgcgtgaa gcgttgctcg gcgcggggac cgagcaggag ttcctcgccg ccggcccgga
     8341 ccgcccggat ttcgggggtt acctggccct caagcgtggc atcgccttcg aaaagggaaa
     8401 ccttcgctgg tcgcgggaag tcctttcgat cctgggagag tcgcgcagtc gtccctgacc
     8461 cgacgtgtgg accggcttca cctgagcgcg gctccgggcc ggtggctggc tcgcatatgc
     8521 tggttttgaa ccgggtaccg gtcgaatacc agtcgcaaaa caaggagagg accccgatgc
     8581 cgactttcgt gatgctgacc aacctgactt ccgagggcgt caagactttg aagagccatc
     8641 ccggccgcgt agccgaggtc aatcgcgagg tcgaagctct gggagccagg gtcgtcgccc
     8701 agtacgcaac ccttgggcag tacgacttcg tgacgatcat cgaagccgaa tcggcagagg
     8761 tgatggccag ggtctcggtc gagatgggtt cgcgcgggac catgtccagc cagaccctca
     8821 gcgcgctccc cgcagagcag ctcgccgaag ttctttgagc gtccgcatcc tcgtccttgg
     8881 cggtggcggc cgcgagcacg ccatcgtcaa ggcgcttgct cgctcgccgc agtcaccgga
     8941 gttgttcgct gccccgggca atcccggcat cgccgccgat gccacctgcc tgcctgacct
     9001 cgaccctgcc gatcctgccg cggtcaccgc gaaagcaagg gaactggaga tcgacctggt
     9061 cgcggtcgga ccggaggccc cgctggtcgc cggcgtggtc gatgccctga gcgatgccgg
     9121 catcgccgcc ttcggcccga gcggtgcggc cgcgcagctt gagggttcca aggcctacgc
     9181 caaggaagcg atggaagagg ccggcgttcc gaccgctggt tacggggttg tgagcgaccg
     9241 ggagagcgcc ctggccaagg tcgccgaaac cggctgcccg gtggtgctga aggccgacgg
     9301 gctggcggca ggcaagggag ttctgatctg cctgaccgaa gaggaagccc gcgaggcgat
     9361 cgaggtcttc ttcaccgaac ggcgcttcgg aaagaccgga gtcgtgatcg aggaattcct
     9421 cgaaggcgag gagctctcgc tgcttgcgat ctgcgacggc gagagggcga tccccctggc
     9481 cccggcccag gattacaagc ggatcggtga gggtgacacc ggcccgaaca ccggcggcat
     9541 gggcagctac tcgccggtcc agaccgtcag caaggatcaa ctggacgaac tgtcggccct
     9601 ggtgcatcag ccgatcgtcg acctgatggc ccgccgcggc caccccttcc acggggtgct
     9661 ctatgcgggc ctgatgatga ccgccgacgg tccgaaggtt ctcgagtaca acacccgctt
     9721 tggcgacccg gagacccagg cggtactccc gcggcttgag tccgacctgg tcgacctctt
     9781 cctttccgcc tcggagccgg gaggcctggc cggaaccgag gtcagctttg ccgatcagtt
     9841 cgcggtgacc gtggtgctgg cctcggccgg ttaccccgcc tcgtcctcga agggtgacgt
     9901 gatcaccgga ctcgacgaag cggcacggtc ggcggaagtg gttcacgccg gcacggcgga
     9961 agcggacgga cggatcgtca ccgccggtgg ccgggtgctg aacctcaccg gtacgggacc
    10021 cgatgctgca accgcgcgcg atcgcgctta cgatgcggct gacgcagtca atttcgatgg
    10081 aaaacagatg cgacgcgaca tcgccgaaag ggctgtccgg cgcgaacgaa accagaaagc
    10141 aacatgagcg aagaaaccca gcagccggca accctttcct cggtcgaaga gctcttcgag
    10201 gaaatcgacg ttgaacagcc ccaggtgggg atcatcatgg gctcgaagaa cgacaagccc
    10261 aaaatgcagc cggccggaca ggcccttgag gacgccggca tcgcctacga ggtgcgggtc
    10321 atgtcggccc atcgcgaccc cgtactggtc gccgagtact gcaccaacgc gaagatgcgc
    10381 ggcctcaagg tgatcatcgc cggggccggc atgtcggccg ccctgcccgg agtcgccgcc
    10441 gcccacaccg agcttccggt gatcggcgtg ccgatcctcg gcaagtccct gggcgggctc
    10501 gacgcgttgc tgtcggtggc ccagatgcct cccggggtgc cggtcgcctg cgtcgcagtc
    10561 gacggcgcca agaacgccgg cgtgctcgcc acccgcatca tcaacctggc cggctagcac
    10621 cgcatgatcg agcgctacac ccgccccggg atgggggcgg tctggaccca gcaggccaag
    10681 ttccagtcct ggctggacgt cgagctggct gccaccgacg cctgggcggc cgagggcgtg
    10741 gtcccgcccg aagccgctgc cgcctgccgc gagcgggcga gcttcacggt cgaggagatc
    10801 aacgcccgcg aggagatcac caaccatgac gtcgcggcct tcgtcgacgt cgtggccgcc
    10861 tcgatcggcg aggagggccg ctggattcac ttcggcctga cttcctcgga cgtgctcgac
    10921 acggccctcg cggtgcagtt gaccaaggcg ggggagattc tggtcgaagg ggcgaagaag
    10981 taccgggacg ccctgatcga ccgggccttc gagttcaagg acacgctggc gatggggcgc
    11041 acccacggag tccacgccga gccgaccacc ttcggccttc gcctggccgg tttcgccttc
    11101 gaggcggacc gcaattacag gcggctccag cgggcgaccg aacaggccgc ccggggcaaa
    11161 ctctcgggcg cggtcggcac ctatgcctcg gtgccgccgg cgatcgagcg ccgggtgatg
    11221 gacgcgctgg gacttaccgc cgaggaaatc gcgacccagg tggtgccgcg cgaccgccac
    11281 gccgagctgc tttcggccat cgccctggcc ggtgccggga tcgaacggtt cgcgaccgag
    11341 gtccggcacc tgcagcggac cgaggtgcgc gaggccgagg agccgttcgc gaccggccag
    11401 cagaaaggct cgtcggcgat gccgcacaag cgcaacccga tccggaccga gcggatgacc
    11461 ggcctggccc ggttgctgcg cggctacgcc cagaccggcc tggagaacgt ggcgctctgg
    11521 catgagcggg acatcagcca ctccggggcc gagcgggtga tcctgcccga cgcaacgatc
    11581 ggcctcgact acatgcatca cgtgatcacc caggtcgctt ccgggctggt cgtccatgtc
    11641 gaccggcttc aggcgaacct cgagcagacc aacggcgcga tgttcagcca gcgggccctg
    11701 accgccctgg tcgaatcggg catgacccgc gacgatgcct accgcacggt ccaggccgcc
    11761 gcccacgaag ccttcgacgg ggacacccat ttccgcgagc tgatcggcaa ggccgccccg
    11821 gagctggacc tcgacgagat cttcgactac aacgcctacc tcacccacct ccccgaagtc
    11881 ttcgaaaggc tcgaagagat ccgctaggca gcagggcccg gcacgggggt atgcaccacg
    11941 cctcgccttc tgggtttagc ctccaggcat gaagctctac acctgcggta caggcaccag
    12001 tggtccggcg ggggtgcatc cctgcgcaaa ggcgggcaag gcgctcgacg acgccggata
    12061 cgagtacgaa ctcaggaagg ttcgcggcta ccggatgttg ccctggacct ggaagagccg
    12121 ggccgaggac cgcaaggagg taaaggagct ctccggcacc aacgaagtgc cgatcctggt
    12181 gctcgacgac ggcgaagtga tcagcgactc gtcggcgatc gccaagtggg cgaaggagaa
    12241 ccctgccccc ggcaggtaag cggccagggg ctggccacgg tgtagacctg atcggcccgt
    12301 ccgaggccag ggccgcggcc gatgcgatcg ctcgcgggtt tcaggacaac gaagtctgga
    12361 tgtggctgct gcccgatgac cgggtccggg cccgggtcga acggcggcag taccggtcgg
    12421 tggtcaggca catcttcgtt tcgcgccggt cgggctggat ttcccttcgc gaagacgggg
    12481 aagcggcggg agcggtgctc tggtacccgc cgggaaccaa gaagcacagc ttcagggaga
    12541 gcctggccga gagcattccc tttctgcccg ccggcctgcc ccggctcggc aaggcaaccc
    12601 ggctgcagaa gctggtcgtt tccaactggc caaaggaacc ccattggtac ctctcggtgc
    12661 tttccgtctc acccgagtcc cagcggcaag gccatggcgg ggcgctgctc gggcccgggc
    12721 tcgccagggc ggaccgccag ggtgtcggct gctggctgga aacccagcgc gaaagcaaca
    12781 ttccctttta cgcccgcttc ggcttcgagc tggtcaagaa gctggaaatc gaggccggcg
    12841 tgaacctctg gctgatgtgg cggcctcccc tcgaggggga ctgaccgcga gggcgctgcc
    12901 gccgggaagc ccgggtggcg gcggatcagc cgggcacagc cgccttcgcc gcgccggtga
    12961 cctcgacgat attgccgtcg aacccggcgg cgtagagact gccggccctg aagcccttgc
    13021 cggcgctact catcgctacc gcggtggtct gcgaaagtcc ggtggcgatc acgcatgcct
    13081 gcctgtccgg agtgatcttc cagacctcgc ccttgccgta ggccgccgtg tagaggttgt
    13141 tgccggagtc atgcgccagc ccgtcgagaa ggacgttgcc tgcctccggc gaggtgaagt
    13201 aagtgaagac tttcgagggg tcgcgggtat cgatcttcgc gatggtcgag ggtgtctcga
    13261 acatctggtt cgcgtagagg tagcgaccat tcctgcctac cgtgaggccg ttggcggagt
    13321 tcaggctggc ccagccgttc tcggtgtcgc cgtcgggcgt gatccggtcg agcttgagac
    13381 cgaggttgtt cgaggcgtag atgtagccgt ccttgccgcg ggcaactccg ttcgccatcc
    13441 cgagagtgtc cgagacgagg ctcttcttct tcgcggcgac gttcacgctg tagagaccgg
    13501 cggtcgggtc gaggtcaccg gtcagtccgt tggccagaag gtctccgtag ccccagagca
    13561 ggcgcttgcc gttccaggcc agcccgccgc cgcccgggtt gccggtcgcc acggtctccg
    13621 gatcggcctt ccccttcgtg taggcccgca gggtgctttg ggtcgtgtca ccgccggaag
    13681 cgaaaagggt tccgccaccg ccgacgatca acgcctcgat cgaaccctgc ccggaataga
    13741 tggtcttctg gctgaaagag ccggtacagg gaggcacttc agccgccgcc gcgactcccg
    13801 aaagggcgaa agccgacgcg atcaggctca cggaagcaac cagaaaagtg cgcatttgcg
    13861 gaggttatac gcagtccggc tttccggctt gtcgattggg tagtttgtcc tttaatggcg
    13921 aattcgattg aagacctcaa gcttgtcaat tccggaaagg tccgcgagat ctacgagatc
    13981 gaggatgacc tcctcctcgt cgcctccgac cgcatctcca tctacgacgt gatcatgccg
    14041 accgcgatac ccgacaaggg caaggttctg acccagatgt ccaacttctg gttcgagaac
    14101 accacggaca tcgtgccgaa ccacttcctc tcgaatgacg tgccggagga agtcgccggg
    14161 cgggcggtcc gggtgcgcaa gctcgagatg tacccggtcg agtgcgtggt tcgcggctac
    14221 ctctccggtt ccggctggaa ggagtaccag gagaccggcg cggtctgcgg ggtcgggctg
    14281 cccgacggcc tgcaggaatc cgacaagttg ccccggccga tcttcacccc ggccaccaag
    14341 gccgaactcg gcgaccacga cgagaacgtg gactttgatc gggccgccga gatcgtcggt
    14401 gaccgccacc tgatggagga actgcgccgg atctcgatcg agatctacga atacggcgca
    14461 aaccacgcgg ccgaaaaggg gatcatcctc gccgatacca agttcgagtt cggcagctac
    14521 gccggggccg agatcgtgct cggtgacgag gttctgactc ccgactcttc gcgctactgg
    14581 ccggccgacc agtacgagcc cggcaagtcg cagccttcct ttgacaagca gtacgtccgt
    14641 gactgggcca acgccgccgg ctgggatcac aacccgccgg ccccggagct gccggaagac
    14701 gtggtcgccc agacccaggc caagtaccgc gaggcctacg agcggatcac cggaagggaa
    14761 ctttgattca ggcccgcgtt ctgatccggc ccaaggaggg catcctcgat gcccagggca
    14821 aggcggtcga aggggcgctg ccggcgcttg gtttctccgg gatcgaacac gtcaggatcg
    14881 gccggatcgt cgaactcgaa gcggacagca ccgatcagct cgaggagctt tgcgagaagc
    14941 tcctcgccaa cccgctgatc gaggattacg agatcgagac cgtcagcgga tgaagtgggg
    15001 tgtgcttcag ttccccgggt cctgcgacga gcgggacgcg ctcaacgcct gccagatcgc
    15061 tgcgacagcc ttgggctctg agggcgggtc cggctcgttg cctcggttgg tttggcatga
    15121 ggagacggat ctgtctgacg tcgacgggat tgtggtgcct ggtggtttct cttacgggga
    15181 ctaccttcgg gcgggggcaa ttgcccggtt ttctccggcg atgaccgcgg tggccgagtt
    15241 cgccgcagcc ggcgggccgg tcctggggat ctgcaacgga ttccaggttc tctgcgagtc
    15301 ggggcttctg cctggcgcgc tgctgaccaa cgagaagacc cggttcatct gccgccaggg
    15361 cgagctgatc gtgggcgagg cggagtcgcc cttcctctcc ggatacgaag ccggccagag
    15421 gatctcgatc cccttcaagc accagtccgg ccgcttcttc gcgccggccg acctgctcga
    15481 ggaaatcgag tcaaacaacc aggtcgcctt ccggtacgcc ccgggccaga atccaaacgg
    15541 ctcgctgaac gacatcgccg gcgtgaccaa cgaggccggc aacgtgatcg gcctgatgcc
    15601 gcaccccgaa catgcggtcg aggcctttgc cggctcgacc gacggtctcg cgctcttcca
    15661 gggcccggcg atcgccgcca ccggagctgt cgcgtgagcg agcaaagcgc gcagcgcgaa
    15721 gacaaaggcg agcagagcgc gcatcgcgct ctgggcctga ccgatcgcga gttcgatctg
    15781 atctgcgaga agatgggccg cgagccgaac ggggtcgaac tggcgatgtt ctcgctgctc
    15841 tggtccgagc actgcgccta caagcactcg cgcaagcttc tgaagaaact gccgaccgaa
    15901 ggtccccgcg tggtcatggg cccgggcgaa aatgccggcg cggtggacat cggcaacggc
    15961 ttttcggtcg ccttcaaggt cgaatcgcac aatcaccctt cggcggtcga accgttccag
    16021 ggcgcagcga ccggtgtcgg cggcatcctc cgcgacgtga tcgcactcgg cgcccgtccg
    16081 atcgcgatcc tcgattcgct tcgcttcggc gagccggaca ccg
//
LOCUS       DAOQKN010000035        15838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_1703_length_15838_cov_6.341443, whole genome shotgun sequence.
ACCESSION   DAOQKN010000035 DAOQKN010000000
VERSION     DAOQKN010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 15838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15838
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1703_length_15838_cov_6.341443"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /gene="nuoH"
                     /locus_tag="PLJ64_03925"
     CDS             <1..883
                     /gene="nuoH"
                     /locus_tag="PLJ64_03925"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HNC92671.1"
                     /translation="KLLGRFQQRYGPNRVGPFGMIQPLADVGKLLSKQAFRPKGAVPL
                     LYELAPALTIISAVLTMAILPFGDVKGDVGFYGIDVPIGILYFFAFSSIAFYGLLLGG
                     WASGSKYSFLGAMRSAAQLISYEIAMGLSLLGVVMMAGSLSLVDIVKAQGEIWYVVPQ
                     FVGFLIFLVGGFAETNRPPFDLPEADAELVAGYGTEYGGMRFGSFMFAEYMEILIVSG
                     IASTMFLGGWMGPGPGWLDPIWMLFKMWMLAMFFIWIRATLPRFRYDQLMSFGWKILL
                     PLATLNVLVTAILVVVT"
     gene            880..1410
                     /gene="nuoI"
                     /locus_tag="PLJ64_03930"
     CDS             880..1410
                     /gene="nuoI"
                     /locus_tag="PLJ64_03930"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HNC92672.1"
                     /translation="MSIRPDEIKVVSRGPEPGGMGGAYRAFGETLRGLRTTMGRLFEK
                     PVTIQYPEEKTPVYPRFRGRHKLHKFEDSGLEKCVGCSLCAAACPAECIRVVAAENDP
                     ENRVSGGERYASVYEINLARCIFCGYCEMACPFDAITMGHDYEMADYNRSDLIFTKEM
                     LLAEPIERTPLRGEGE"
     gene            1410..2024
                     /locus_tag="PLJ64_03935"
     CDS             1410..2024
                     /locus_tag="PLJ64_03935"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNC92673.1"
                     /translation="MEEIAFFIGAIGALGAAIAVIAVRNPFYSVLALVIHLVFLAGIF
                     LLLMAQFLAAALIVVYAGAVMVLYVFVAAYVGGIEEPKWDSSPELRIVGPILAVALFI
                     AVSIAILGSGLSALGTPGPDDVPSGFGTPEAVGHLMMEDFLIAFEAASLLLLVAAVAA
                     IVLAGRRREDRPSDLSKRKSMVARDENVNAAIAGDPAAGRSEEA"
     gene            2024..2323
                     /gene="nuoK"
                     /locus_tag="PLJ64_03940"
     CDS             2024..2323
                     /gene="nuoK"
                     /locus_tag="PLJ64_03940"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNC92674.1"
                     /translation="MDIEWYLILSALLFTVGFIGVLVRRNPVVILLCLELMLSAGALA
                     LLAFSRMLGNQDGQVFVLIVLVVAACEVVVGLGLVVAMYRRRLPLDVDEMKELKG"
     gene            2320..4326
                     /gene="nuoL"
                     /locus_tag="PLJ64_03945"
     CDS             2320..4326
                     /gene="nuoL"
                     /locus_tag="PLJ64_03945"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNC92675.1"
                     /translation="MTATWAWLVLLLPLLGSMTIGLTFRVLPARTAGLIGVTAIVAAF
                     VCGILALIGLQGEPSESRHVASSLWEYASVGGLSIDLGIYVDPLSIFMVLVVTGVSSL
                     IHIYSYSYMKSDQGYHRFFSYLNFFVFSMLVLVLAGNFIMLIVGWALVGFASYALISY
                     WYRRNTATKAGMKAFVINVIGDIGLYLAAILIFKNIGSVDYGTVFEKAPEVFTTNEWE
                     IVAICLCLLVGAFAKSAQMPFHTWLADAMEGPTPVSSLIHAATMVTAGVYLIARCHSL
                     FELAPTAADVSAWVGLVTLLIAGTIAIAVTDLKRIIAYSTMSQIGYMFVGVSIGAYSA
                     GMFHLMTHAFFKALLFMAAGSFISAMANMQDIDRMKGFRKAMPFTAAMMIIGALALSG
                     FPGTSGWFSKDEIIDFAHFRGGMYDWMWVGMLVGAFMTAVYSFRIIFRILPGPPCEEA
                     EYLEEHGHVIHGEPKNPATGELEDTEVGYPGEEHHIAEQAGGMKFAMAVLGVLALIAG
                     VLQIPGLDDVVTKFLEPTFENSVFSHMHPTTSESWIGLGEGALISLAGIATAWLLYIK
                     RPDLPVRFRERFRWIHTLTVNKWYGDEIIDFLIVNPVKAIGRFCNRAFERFVIDGATT
                     GVAGLTRSAGAAVRDMQSGLLRGYAVLMLFGILAIVIYFLIRSI"
     gene            4326..5831
                     /locus_tag="PLJ64_03950"
     CDS             4326..5831
                     /locus_tag="PLJ64_03950"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNC92676.1"
                     /translation="MLSSIIWTPLLFGLIGLLVPRRFAAWVAALGAVITLGLSIGVLA
                     GFDPDGGMQFVTDVSWIPGLGVDYSLGIDGVSIFLVMLTAIAWIPAIGFSALRGTERP
                     ALFYLMLLAGETSTLGAFLAQDLLLFVLFFDLMIVPFYFLFGIWGRDRGGESDPEGSP
                     GNGPVTVQSATLKMIVFTLIGSLLMLVGAIAAAVIAADGGELTFSIQTIVARGLPEGS
                     QNWIFWFFAAAFLVKMPAFPLHGWMPDSYRAAPLPALAVFSAVLSKVGAYGFLRVVLP
                     MMPDAADLFQTTMLVLAIASILYGSAMAFTKTDLRLVLGFSSVAQLGFITAGIFAFNE
                     SGAEGAVLQMVNHGLVVVPAFLIVAMISERTGSEELGPMGGLAKKAPVFAVIFLIVSM
                     ATLAIPASANFIGEFFILNGLFQVDAAWAIVASVGVALAAFYALRMYQLTMHNPLPDG
                     SDSKEISLRDALVVIPMVAIVVVLAFCPQLIIHGSEPAVDSSIQVVKEVAE"
     gene            5828..7366
                     /locus_tag="PLJ64_03955"
     CDS             5828..7366
                     /locus_tag="PLJ64_03955"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HNC92677.1"
                     /translation="MSFDAPAMDYAGLSPVIALTTGLVVIVLASVFDSVKRFGPFLAL
                     LTFAVTAGCLIWQWSADIDLVSGSLRLDGLAATLSILVVIAAAVSVLLAIGDPSETQA
                     GRSDWLALLTGSVLGMVILAQATSMLTFFVGLELLSIPLYALCGTNLRRRESLESGLK
                     YLIVGSVGSATLLYGLAMIYGASGATGFQAISDGLQAGDGMLSDSLTLVGVGLVAVGL
                     AFKVSIAPFHQWTPDVYQGAPTPVTAFMAVATKAAAFAVFIRFFLVALGPLADQWDVM
                     LAALAALSIVVGNVGALAQTSLKRMLGYSGIAQAGYMLAGIVTATQAGVDALVFYLAA
                     YVAMNVGAFAVVVARERQTGFSDDISTLRGLGREKPLLAWPLTISMLALAGLPPTVGF
                     IGKLYLIEALVDDDWTWLAVMIAIGTMISLGYYLRVVATVWMSPAEESEAAAASIDTA
                     ALPPQIAGAQADPFTDSRADRRWYLVAPALIAMAAALFFGVIPQPLVDFAQHAGNALS
                     VWIS"
     gene            complement(7960..8592)
                     /locus_tag="PLJ64_03960"
     CDS             complement(7960..8592)
                     /locus_tag="PLJ64_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92678.1"
                     /translation="MERITSRLSVAREFRLTIAKDLSDPENHSDRIGREFLVGNPDHQ
                     VSASFEKELPFHIELLAERIAVEFVPVQFQNHSEPAPEAVNLPAQHLHIHFRHRQLVF
                     HPDQQEPALEYRPDPPKFRNPEGKQGTKFGCAVPVRDFPVAVLKIRFPKKPKPASFEE
                     SESQFLLGGPGSELDKCQCWGNQSDTVPFPGFVGTEAATNDAHHGSAKSR"
     gene            8647..9567
                     /locus_tag="PLJ64_03965"
     CDS             8647..9567
                     /locus_tag="PLJ64_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92679.1"
                     /translation="MVWILLAIVVSVAAGIFGERRWPDKAGTGSRRGLLLILYFVLPP
                     IVFFNLVHVELSFGGGAGIAAGILTSICVGLLAWTLAVPVLKLSRPVAGAVIVSSILA
                     NTSYLGYPLVYTLMGGDALTDAVLFDVVVSYLTLMIGAFGVGAAFGTKAGEGFRERLK
                     AFFFKNPLLVAAILGLLAPESLAPDIAVEISRILVVLPLVVGFFAVGAILTEEVRDGV
                     MKVPPRPHRPLGAVIFCRLIAAPSLLLLFTLPVSGVPSSYYLLAVMPTGLNSMIVGHA
                     YGLDLRTCAEAIVYSTTIVLVGVAGYTLIA"
     gene            9605..9973
                     /locus_tag="PLJ64_03970"
     CDS             9605..9973
                     /locus_tag="PLJ64_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNC92680.1"
                     /translation="MVHHVALEVRPDLMAAEGKFWVAAGFEPVAAPEGLGAGFDWYER
                     EGTQIHVMAVGQPGEPSRRGHVAIVAPDFEATLARLGEEGFEVSETRNLWGARRAKGR
                     TPAGHLVELMEFPPRPAEAG"
     gene            9970..11481
                     /locus_tag="PLJ64_03975"
     CDS             9970..11481
                     /locus_tag="PLJ64_03975"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNC92681.1"
                     /translation="MIRRALLPAAILAAALIPGPASPAAAAGPEELVPDPDTPVVLIV
                     LDELPTAELMTADGRRINDIRFPNIARFARGATWYRDNVAAGDFTAWAVPGILTGNLV
                     NELTLPTTAAQPDNFFTWLGPGRTVHNMETVTELCPVEICPDGDQGGAPGVVRADAFI
                     LAKFRPFAPNEVKQWIEGIPAGPGTLSFVHVEVPHQPLRFIPSGQAYKPGPLIMPTDT
                     NLNGWTVGDAAVAFVQHRHLLQTGYADRIVGRILDRIEENGDFDDAMIVLTADHGISY
                     DPDDLRRDLSPTNMGGTVNPPLLIKYPGQSEGVISTASTQSIDIYPTIARQLGVAIPP
                     TDGQPVDEAGADRVMTVMKNQMRQQEVTAEEVRSDRRGALKDQFRRLGPGDLWHLGPR
                     SSLIGTRSGSRPGLKGASYRLYLSDERLRRADGDNNFVPSLISGQVAGVGPGRLMALA
                     WNGTIVATTRSFTYQGKVRFGFMVRPAVMRRGHNRINVLAAGAGGSLRRVPED"
     gene            11596..13236
                     /locus_tag="PLJ64_03980"
     CDS             11596..13236
                     /locus_tag="PLJ64_03980"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HNC92682.1"
                     /translation="MRYPVESFTPEEQASLAPYFTNLDQPVFALTNLPETVKGALFAR
                     YSRYGGTLRRLYLDEFAADAAAADPEGRLRPFDGEEGQRAAKLYESVFVGFGDDSIAQ
                     LGGAHVACEWVSNILTKVLQRGRLAAYLEQSTRYIAYDQEMPGGGYRYLSGDALDGQF
                     EAAMNDIFLIYSDSLTRVEAWAAERWPRGDDEPEGPWRRSIKAKALDLLRGLLPASTL
                     SHVGIYASGQAWEQLLFRMMASPLPEARRFGGMILDELNKVIPSFVSRVSRPDRGGEW
                     ISFLEQRRQATEDWVERLGLTDRDSEDSGPTVELGHVHGTEDDLLAACLYEESDAPES
                     RIRERIARLDSRQREELMAAMIGERRNRRHRPGRGFESVSYRFEIVSDYGAFRDLQRH
                     RMLTCQWQTLTPDLGAGVPAEVVEAGVGGAYEEALEISRNEYERLRDAGHPEAAPYAL
                     CLGYRIRYILDLNAREAMQLIELRSGREGHPTYRAVAQAMHDEIAEVHPAIAGAMKHL
                     DTSMEPRLERMLSEIRTHHKQLAAAQTSKAPPGFSELG"
     gene            13346..14173
                     /locus_tag="PLJ64_03985"
     CDS             13346..14173
                     /locus_tag="PLJ64_03985"
                     /inference="COORDINATES: protein motif:HMM:NF015500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HNC92683.1"
                     /translation="MLSARIPPALPCALAFLLLAMMVVFAACSSDPDQSEAKPARWQP
                     KPTTAAWQFQLQGKIDTSIDAPVFEVDGFNVPKSTVTRLHSMGRKVICYIDVGSWENY
                     RPDAKRFPGSVIGRKYEGYPNERWLDIRRYRKFAGPIKSRIRMCARKGFDGLEPDNIN
                     GWENPTGFPLTARDQLRFNRWIAKVAHNNGLAVGLKNDGRQARKLVGAFDFAVVEQCF
                     QYSECGQFLPFIRRGKAVYSVEYERPNSAFCARAETLGFSSIGKEYDLFARPWRPCA"
     gene            complement(14313..15095)
                     /locus_tag="PLJ64_03990"
     CDS             complement(14313..15095)
                     /locus_tag="PLJ64_03990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HNC92684.1"
                     /translation="MALADSYDGFLIDLDGVVWLGHEPIEGAAKAIAALAAAGKPFVF
                     VTNSPRVTPAEQARILREQGIEADEDRVVTAGDTLLELTRKEIGEAGKVYAIGTSGFL
                     EQVEGAGFQLLPAERWQEAEAVLVTGHPRFDYDELKAASMAARAGAFLATIGRDPTMP
                     MPDGLWPGTGSIVAAVEAASGRTAVVAGKPERPIFDLGVRRLGLPEGARVAMIGDRLD
                     TDVGGAQKAGLEGILVAGNESHNPETRVTPNHNISSLLDLTD"
     gene            15174..15665
                     /locus_tag="PLJ64_03995"
     CDS             15174..15665
                     /locus_tag="PLJ64_03995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNC92685.1"
                     /translation="MTGNRTNTEGPLPFETALEDHGPRLHAWLLARVGPSRADDVFQE
                     TMLAALAAWDRVPVQSVRAWLFTIARNKAIDEQRRDANRPRPEGEVDSWPSAEVAERL
                     EDPVWDQVRELPEKQRIAVTLRFRGDLNHREIGTVMEISEAAARRNVFEGVKRLRERI
                     EDE"
     gene            15658..>15838
                     /locus_tag="PLJ64_04000"
     CDS             15658..>15838
                     /locus_tag="PLJ64_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92686.1"
                     /translation="MSEIEARLAAGEVAGTGSNWEEIRTSLLRQADREGAIDLAYERH
                     DTPFGTMVIVAGEGGI"
BASE COUNT         2406 a         5347 c         5058 g         3027 t
ORIGIN      
        1 caagctgctc ggtcgtttcc agcagcgcta cggcccgaac cgggtcggtc ccttcggcat
       61 gatccagccg ctggccgacg tcggcaagct gctctccaag caggccttcc ggcccaaggg
      121 cgccgtgccc ctgctctatg aactggcccc ggccctgacc atcatctccg ccgttttgac
      181 gatggcgatc ctgcccttcg gggacgtcaa gggtgacgtc ggcttctacg ggatcgacgt
      241 gccgatcggg atcctctact tcttcgcctt cagctcgatc gccttctacg ggttgctgct
      301 cggaggctgg gcctccggct ccaagtactc cttcctcggg gcgatgcgca gcgcagccca
      361 gctgatctcc tacgagatcg cgatgggcct ctcgctgctc ggcgtggtga tgatggccgg
      421 ctcgctttcc ctggtcgaca tcgtcaaggc ccagggcgag atctggtacg tcgtgcccca
      481 gttcgtcggc ttcctgatct tcctggtcgg tggtttcgcc gaaaccaacc gcccgccctt
      541 cgacctgccc gaagcggacg ccgagctggt cgccggctac ggcaccgagt acggcggcat
      601 gcggtttggc tccttcatgt tcgccgagta catggagatc ctgatcgtct ccggcatcgc
      661 ctcgaccatg tttctcggcg gctggatggg ccccggcccg ggctggctcg atccgatctg
      721 gatgctcttc aagatgtgga tgctggccat gttcttcatc tggatccgcg ccaccctgcc
      781 ccgcttccgt tacgaccagc tgatgagctt cggctggaag atcctgcttc ccctcgcgac
      841 cctgaacgtc ctcgtcacgg caatcctggt ggtggtcaca tgagcatccg tcccgacgag
      901 atcaaggtcg tctcccgcgg tcccgaaccg gggggaatgg gtggcgccta ccgtgccttc
      961 ggcgagaccc tgcgcggcct tcgcaccacg atgggccggc tcttcgagaa gccggtcacg
     1021 atccagtacc cggaagagaa gacaccggtc tacccgcgct tccgcggacg ccacaagctt
     1081 cacaagttcg aggattcggg gctcgagaaa tgcgtcggct gctcgctttg cgccgccgcc
     1141 tgcccggccg aatgcattcg cgtggtcgcg gccgagaacg atcccgagaa ccgggtctcc
     1201 ggtggcgagc gctacgcctc ggtctacgag atcaacctcg cccgctgcat cttctgcggc
     1261 tattgcgaga tggcctgccc cttcgacgcg atcaccatgg gccacgacta cgagatggcc
     1321 gactacaacc gctcggacct gattttcacc aaggagatgc tgctggccga gccgatcgaa
     1381 cggactccgc tgcggggaga gggcgagtag tggaagagat cgccttcttc atcggcgcga
     1441 tcggcgccct cggtgcggca atcgcggtga tcgcggtccg gaacccgttc tacagcgtgc
     1501 tggccctggt catccacctg gtcttcctgg ccgggatctt cctgctcttg atggcccagt
     1561 tcctggccgc cgccctgatc gtcgtctacg ccggtgcggt gatggtgctc tacgtcttcg
     1621 tcgccgccta cgtcggcggc atagaggagc ccaaatggga ctcctcgccg gagctcagga
     1681 tcgtcgggcc gatccttgcg gtggcccttt tcatcgcggt ttcgatcgcc attctcggct
     1741 ccgggctttc cgcgctcggc acccccggcc cggatgacgt gccttccggc ttcggcacgc
     1801 cggaagcggt cggccacctg atgatggagg acttcctgat cgccttcgaa gcggcctcgc
     1861 tgcttctgct ggtcgctgcg gtcgcggcaa tcgtgctggc cggccgccgc cgtgaggacc
     1921 gtccttccga tctttcgaag cgcaagtcca tggtcgcccg ggacgagaac gtgaacgccg
     1981 cgatcgccgg ggatccggcc gccggcagga gcgaggaggc ctgatggaca tcgagtggta
     2041 cctgatcctt tcggccctgc tcttcacggt cgggttcatc ggggtgctgg tgcgccgcaa
     2101 cccggtggtg atcctgctct gcctcgagct catgctgagc gccggggccc tggccctgct
     2161 cgctttcagc cgcatgctgg gcaaccagga tggccaggtc ttcgtgctga tcgtgctcgt
     2221 ggtggcggcc tgtgaggtcg tggtcgggct cgggctggtc gtcgcgatgt atcgccgacg
     2281 gctgccgctc gacgtcgacg agatgaagga gctcaaggga tgaccgccac ctgggcctgg
     2341 ctcgtcctgc tgctgcccct gctcggttcg atgaccatcg gactgacctt cagggtgctg
     2401 ccggcccgca ccgccgggct gatcggcgtc accgcgatcg tcgccgcctt cgtctgcggc
     2461 atcctcgccc tgatcggtct gcagggtgaa ccttcggagt cccggcacgt cgcctcgagc
     2521 ctctgggagt acgccagcgt gggcggactt tcgatcgacc tcgggatcta tgtcgacccg
     2581 ctttcgatct tcatggtgct ggtcgtcacc ggcgtttcca gcctgattca catctactcg
     2641 tacagctaca tgaagtcgga tcagggctac caccgcttct tcagctacct gaacttcttc
     2701 gtcttttcga tgctggtcct ggtcctggcc ggcaacttca tcatgctgat cgtcggctgg
     2761 gccctggtcg gcttcgcttc ctacgcgctg atcagctact ggtaccgccg gaacacggcg
     2821 accaaggccg gcatgaaggc cttcgtgatc aacgtgatcg gcgacatcgg cctttacctc
     2881 gcggcgatcc tgatcttcaa gaacatcggt tcggtcgatt acggcacggt cttcgagaag
     2941 gcgccggaag tgttcaccac caacgagtgg gagatcgtcg cgatctgcct ctgcctgctg
     3001 gtcggcgcct tcgccaagtc cgcccagatg ccgttccaca catggctcgc cgacgccatg
     3061 gaaggcccga ccccggtttc ctccctgatc cacgcggcga ccatggtcac ggccggcgtc
     3121 tacctgatcg cccgctgcca ctcgctcttc gagcttgctc cgaccgcggc ggacgtctcc
     3181 gcctgggtcg ggctggtcac gctgctgatc gcgggcacga tcgcgatcgc ggtcaccgac
     3241 ctgaaaagga taatcgccta ctcgaccatg agccagatcg gctacatgtt cgtcggggtc
     3301 agtatcgggg cctactcggc cgggatgttc cacctgatga cccacgcctt cttcaaggca
     3361 ctgctcttca tggcggccgg ttcgttcatc tcggcgatgg cgaacatgca ggacatcgac
     3421 aggatgaagg gctttcgcaa ggcgatgccg ttcaccgcgg cgatgatgat catcggggcc
     3481 ctggcccttt ccggcttccc cggcacctcg ggctggttct cgaaggacga gatcatcgac
     3541 ttcgcgcatt tccgcggcgg catgtacgac tggatgtggg tcggaatgct ggtcggcgcc
     3601 ttcatgaccg cggtctactc gttccggatc atcttccgga tcctgcccgg cccgccctgc
     3661 gaggaggccg agtacctcga ggagcacggt cacgtgatcc acggtgagcc gaagaacccg
     3721 gccaccggcg agctggagga caccgaggtc ggctaccccg gcgaggagca tcacatcgcc
     3781 gagcaggccg gcgggatgaa gttcgcgatg gcggtgctcg gcgtgctggc cctgatcgcc
     3841 ggcgtgcttc agatccccgg gctcgacgac gtggtcacca agttcctcga accgaccttc
     3901 gagaactcgg tcttctccca catgcatccg accaccagtg aatcctggat cggcctcggc
     3961 gagggtgccc tgatttcgct ggccggcatc gccaccgcct ggctgctcta catcaagcgg
     4021 cccgacctgc cggtccggtt ccgcgagcgc ttcaggtgga tccacaccct cacggtcaac
     4081 aagtggtacg gcgacgagat catcgatttc ctgatcgtga atccggtcaa ggcgatcggt
     4141 cgcttctgca accgcgcctt tgagcgtttc gtgatcgacg gtgccaccac cggggtcgcc
     4201 ggacttaccc gttcggccgg cgccgcggtt cgtgacatgc agtccggcct gcttcgcggc
     4261 tacgccgtgc tgatgctctt cggcatcctc gcgatcgtca tctacttcct gatccggagc
     4321 atctgatgct gagttcgatc atctggactc cgcttctctt cggcctgatc gggctgctgg
     4381 tcccccggcg tttcgccgcc tgggtcgcgg cgctcggcgc ggtgatcacg ctcggcctct
     4441 cgatcggagt gctggccggc ttcgaccccg atggcggcat gcagttcgtc accgacgtct
     4501 cctggatccc cggcctcggc gtcgattaca gcctcggcat cgacggggtc tcgatcttcc
     4561 tggtcatgct gaccgcgatc gcctggatcc ccgcgatcgg tttctcggcc ctgcggggga
     4621 ccgaacggcc ggccctcttc tacctgatgc tgctggccgg cgaaacctcg accctcggcg
     4681 ccttcctcgc ccaggacctg ctgctcttcg tcctcttctt cgacctgatg atcgtgccct
     4741 tctacttcct cttcgggatc tggggccgcg accggggtgg cgaaagcgac ccggaaggaa
     4801 gtcccggaaa cggaccggtc acggtgcagt cagcgaccct gaagatgatc gtcttcaccc
     4861 tgatcggctc gctgctgatg ctggtcggag cgattgccgc cgcggtgatc gcggccgacg
     4921 ggggagagct gactttctcg atccagacga tcgtcgcccg cggcctgccc gagggttccc
     4981 agaactggat cttctggttc ttcgctgccg ccttcctggt caagatgccg gccttcccgc
     5041 tccacggctg gatgcccgac tcctaccggg cggcgccgct gccggccctg gcggtcttct
     5101 ccgccgtgct ctcgaaggtg ggcgcttacg gcttcctcag ggtcgtgctg ccgatgatgc
     5161 ccgacgctgc cgacctcttc cagacgacga tgctggtcct cgcgatcgcc tccatcctct
     5221 acggctcggc gatggccttc accaagaccg acctgcggct cgtgctcggt ttctcttcgg
     5281 tcgcccagct gggattcatc acggccggca tattcgcctt caatgaatcc ggtgcggagg
     5341 gcgcggtgct gcagatggtc aaccacggac tggtcgtggt ccccgccttc ctgatcgtgg
     5401 cgatgatcag tgaacggacc ggctccgagg agcttggccc gatgggcgga ctggccaaga
     5461 aggcgccggt cttcgcggtg atcttcctga tcgtctcgat ggcaaccctg gcgatccccg
     5521 cctcggccaa cttcatcggc gagttcttca tcctcaacgg gctcttccag gttgacgcgg
     5581 cctgggccat cgtcgcctcg gtcggtgtcg ccctggccgc cttctatgcg ctccggatgt
     5641 accagctgac catgcacaac ccgctgcccg acggctccga ctcgaaggag atctcgctgc
     5701 gggatgccct ggtggtgatc ccgatggtcg ccatcgtggt cgtgctggcc ttctgcccgc
     5761 agctgatcat ccacggttcc gagccggcgg tggactcttc gatccaggtt gtgaaggagg
     5821 tggcggagtg agcttcgacg cacccgccat ggactacgcc ggcctctcgc cggtcattgc
     5881 tctgaccacc ggcctggtcg tgatcgtgct ggcttccgta ttcgactcgg tcaagcgctt
     5941 cggtccgttc cttgccctgc tgaccttcgc ggtcacggcc ggctgcctga tctggcaatg
     6001 gagcgccgat atcgacctgg tctcgggttc gctcaggctg gacggtctgg ccgcgaccct
     6061 ttcgatcctc gtcgtgattg cggcggcggt ctcggtgctg ctggcgatcg gtgatccctc
     6121 cgagacccag gccgggcgaa gcgactggct ggcgcttttg accggatcgg tactcggcat
     6181 ggtcatcctc gcgcaggcga ccagcatgtt gaccttcttc gtcggcctcg agctgctctc
     6241 gatcccgctt tacgcgctct gcggcaccaa tctgcgccgt cgcgaatcgc tcgagtccgg
     6301 gctcaagtac ctgatcgtcg gttcggtcgg ttcggccacc ctgctttacg gcctggcgat
     6361 gatctacggc gcctccggcg cgaccggctt ccaggcgatc agtgacgggc tgcaggccgg
     6421 cgacggcatg ctttcggact ctctgaccct ggtcggagtc ggcctggtcg cggtcggcct
     6481 cgccttcaag gtttcgatcg cgcccttcca tcagtggacg ccggatgtct accagggcgc
     6541 cccgaccccg gttacggcct tcatggcggt tgcgaccaag gccgccgcct tcgccgtctt
     6601 catccgcttc ttcctggtcg cactcgggcc gctggccgat cagtgggacg tgatgctggc
     6661 cgccctggcc gcgctctcga tcgtggtcgg caacgtcggc gccctcgccc agacttcgct
     6721 gaagcgcatg ctcggctatt ccggcatcgc ccaggccggg tacatgctgg ccgggatcgt
     6781 caccgcgacc caggccggtg tggacgccct ggttttctac ctcgccgcct atgtcgcgat
     6841 gaacgtcggt gccttcgcgg tggtcgtggc ccgcgagcgt cagaccggct tctccgatga
     6901 catctcgacc ctgcgcggtc tcggtcgcga aaagcccctg ctcgcctggc ctttgacgat
     6961 ctcgatgctg gccctcgccg gcctgccgcc gaccgtcggc ttcattggca agctgtacct
     7021 gatcgaggcc ctggtcgatg acgactggac ctggctggcg gtgatgatcg cgatcggcac
     7081 gatgatctcg ctcggctact acctgcgggt ggtcgccacc gtctggatga gcccggcgga
     7141 agagtccgaa gccgccgctg cttcgatcga caccgccgcc ctgccgcccc agatcgccgg
     7201 cgcccaggcc gaccccttca ccgactcccg ggccgatcgc cgctggtacc tcgtcgcccc
     7261 ggcgctgatc gccatggccg ccgccctctt cttcggcgta atcccccagc ccctggtcga
     7321 cttcgcccag cacgccggca acgccctaag cgtctggatc tcctaaccgc agtcgggcga
     7381 gtcttcgctc gccctccagg tctcaacccg ccccgcatct gggttgaagt tattcgttcc
     7441 atgtgcggat aacttcaacg ctgtcggtcg cccgtcgttg ccgcactagg tcgaactgcc
     7501 acaacggagg cccggaatcc gggcgtcgtt gcacaatcgt cacacctata gagccacatg
     7561 tgagcctttt cgcggcaagt tggggggatg aactcccgaa gatcacccag gcagcatccg
     7621 cgggaactac tgcggaggct ctatggcttt atgtcacggc atcatttcgt ggctcatcgg
     7681 gacgagctgg ccgcactggg gtttctccgg ggtcagatcg acagctggat cagaaacggt
     7741 cgattgatcc gggttctgag aggcgtttat gtctacggac gggatgttga gtccaatagc
     7801 gcggcctgga gggcggcgac actcgcggct ggttccggag ctgtgttgac cgggcgcagc
     7861 gcctgcgaga agtgggggat ggtcagagag ccgctcgggc tgccgaaggt catcgatgtt
     7921 gcttccccaa ctggacagac gagaaatctg cgcgggcaat caccggcttt tcgccgaacc
     7981 gtggtgcgca tcgttcgtcg ctgcttcagt cccgacgaaa ccagggaaag ggacggtgtc
     8041 gctctggttt ccccagcact ggcacttgtc gagttcgctg cccgggccac cgagaaggaa
     8101 ctgcgattcg ctttcctcga agcttgccgg cttcggcttt ttgggaaaac ggatcttgag
     8161 tactgccacc ggaaagtctc gaacaggaac ggcgcagcca aacttcgtcc cctgcttgcc
     8221 ctctgggttc cggaacttgg gcggatcagg tcggtactcg agggctggtt cctgctggtc
     8281 tgggtgaaac acaagctgcc gatgccggaa gtgaatgtga aggtgctggg cagggaggtt
     8341 gactgcctct ggcgccggct cggagtgatt ctggaactgg acgggaacga attccacagc
     8401 gatccgctcc gcaagaagct cgatgtggaa aggcagctct ttctcgaagc tcgcggatac
     8461 ctggtgatcc gggttaccta caaggaattc gcggccgatc ctgtcggagt ggttctccgg
     8521 atcgctgaga tccttcgcaa tcgtgaggcg gaactcacgg gcgacagaca gtcttgaagt
     8581 tattcgttcc atgtgcggat aacttcaacg ctgtcatggg tgcgcggtca tctctagggt
     8641 tagccggtgg tctggattct tctggcgata gtcgtttcgg ttgcggccgg gatctttggg
     8701 gagcggcgtt ggcctgacaa ggcggggacg ggttcacggc gggggttgct gctgatcctg
     8761 tatttcgtcc tgccgccgat cgtctttttc aacctggtcc acgtcgagtt gagcttcggt
     8821 ggcggggcgg ggatcgcggc cggcatcctc acctcgatct gcgtcgggtt gctggcctgg
     8881 accctggcgg tgccggtcct gaaattgagc cggccggtag ccggggcggt gatcgtttcc
     8941 tcgatcctcg ccaacacctc gtatctcgga tacccgctgg tctacacgct gatgggcggc
     9001 gacgcgctga ccgatgccgt cctcttcgac gtcgtggtca gctacctcac cctgatgatc
     9061 ggagctttcg gggtcggcgc ggccttcggg accaaggccg gagagggatt ccgcgagcgg
     9121 ctcaaggcct ttttcttcaa gaacccgctg ctggtcgccg cgatcctcgg cctgctggcg
     9181 cccgaatccc tcgccccgga catcgcggtg gagatttccc ggatcctcgt ggtgctgccc
     9241 ctcgtggtcg gcttcttcgc agtcggggcg atcctcaccg aagaggtccg ggacggggtg
     9301 atgaaggtgc caccccgccc gcaccggccg ctcggcgcgg tgatcttctg ccgcctgatc
     9361 gccgccccgt cgctcctgct gctcttcacc cttccggtca gcggcgttcc ttcctcctac
     9421 tacctgcttg ccgtgatgcc gaccggcctc aattcgatga tcgtcggcca cgcatacggt
     9481 ctcgatctcc gtacctgcgc cgaggccatc gtctactcga ccaccatcgt cctggtcggc
     9541 gtcgccggct acacgctgat tgcctgatcc gcctttaccg gcctcgatcg gggaagatcg
     9601 ggccatggtt caccacgttg cgctggaggt gcggccggat ctgatggcgg ccgagggaaa
     9661 gttctgggtc gctgccggct tcgagccggt ggccgcaccg gaaggactcg gggcgggttt
     9721 tgactggtat gaacgggagg ggacacagat tcacgtgatg gctgtcggcc aaccgggcga
     9781 gccttcacgg cggggccacg tggcgatcgt cgcccccgat ttcgaggcga ctctggcccg
     9841 tctcggcgag gaggggttcg aggtcagcga gacccgcaat ctctggggag ccaggcgggc
     9901 caagggccgg acccccgccg gccacctggt cgagctgatg gagtttccgc cccggccggc
     9961 agaggccgga tgatcaggcg ggcccttctc ccggcggcga tcctcgccgc cgccctgatc
    10021 cccggacccg cgagcccggc cgcggcagcc gggccggagg agctggtgcc cgaccccgac
    10081 acgccggtcg tcctgatcgt gctcgacgag cttccgaccg ccgagctcat gaccgctgac
    10141 ggcaggcgaa tcaacgacat ccgcttcccc aacatcgccc gattcgcgcg gggggcgacc
    10201 tggtaccgcg acaacgtcgc cgccggcgac ttcaccgcct gggccgtgcc gggcatcctc
    10261 accggcaacc tcgtcaacga attgaccctg ccgaccacag cggcccagcc ggacaacttc
    10321 ttcacctggc tcggaccggg acggaccgtc cacaacatgg aaaccgtgac cgaactctgc
    10381 ccggtcgaaa tctgccccga cggcgatcag ggcggagcgc ccggagtcgt ccgggccgat
    10441 gccttcatcc tggccaagtt caggcccttc gccccgaacg aggtcaagca gtggatcgaa
    10501 ggcattccgg ccggtcccgg caccctctcc ttcgttcacg tcgaggtgcc gcatcagccg
    10561 cttcgcttca tcccctccgg ccaggcttac aagccgggcc cgctgatcat gcctaccgac
    10621 accaacctga acggctggac ggtcggggac gccgcggtcg ccttcgtcca gcatcgtcac
    10681 ctgctccaga ccggctacgc cgaccggatc gtcggccgga tcctcgaccg gatcgaggag
    10741 aacggcgatt tcgacgacgc gatgatcgtg ctgactgcgg accacggaat ctcgtacgac
    10801 ccggatgacc tgcggcgcga cctgagcccc accaacatgg gtggcaccgt caacccgccg
    10861 ctcctgatca agtatccggg acagagcgag ggcgtgatct cgaccgcttc cacccagtcg
    10921 atcgacatct accccacgat cgccaggcaa ctcggggtcg cgatcccgcc gaccgacggc
    10981 cagccggtgg acgaagccgg ggcagaccgg gtgatgaccg tgatgaagaa ccagatgcgc
    11041 cagcaggaag tcaccgcgga ggaggtccga agcgaccgcc gaggggccct gaaggaccag
    11101 ttccggcggc tcgggcctgg cgatctctgg catctgggcc cgcgcagctc gctgatcgga
    11161 acccggtccg ggtcgagacc gggcctgaag ggagccagct accggctcta cctgtccgat
    11221 gagcgactta gaagggccga cggtgacaac aacttcgtgc cgtcactgat ctccggccag
    11281 gttgccggcg tcgggcccgg ccggctgatg gccctggcct ggaacgggac gatcgtcgcc
    11341 accacccgca gcttcaccta ccagggcaag gtccggttcg gcttcatggt caggccggcg
    11401 gtgatgcgcc gggggcacaa ccggatcaac gtcctggcgg ccggggcggg cgggtctctg
    11461 cgccgggttc ccgaggactg aacccgcccc ttcggggccg cgtgcggctg gcccggcgac
    11521 gccggacttc gcgatcgggt ccgggtctac ataacccggc agtcgagcgt cgggcgcgcc
    11581 tgttaggttc gacgggtgcg ctacccggtc gagagcttta ctccggagga gcaggcctcg
    11641 ctcgctccct atttcaccaa ccttgaccag cccgtgttcg cgctcacgaa cctgcccgaa
    11701 acggtcaagg gagccctctt cgcccgctat tcccgttacg gggggaccct gcgaaggctc
    11761 tatctcgacg agttcgcagc cgacgccgcg gccgccgacc cggaagggcg cctcaggccc
    11821 ttcgacgggg aggaaggcca gcgtgcggcg aagctctacg agagcgtctt cgttggtttc
    11881 ggtgacgatt cgatcgccca gctcggtggt gcccacgtcg cctgcgaatg ggtgtcgaac
    11941 atcctgacca aggtgctgca gcgcggtcgg ctggctgcct acctcgagca gtccacccgc
    12001 tacatcgcct acgaccagga gatgcccggt ggcggctacc gctacctgag cggcgacgct
    12061 ctcgacggcc agttcgaagc ggcgatgaac gacatcttcc tgatctactc ggattcactc
    12121 acccgggtgg aagcctgggc cgccgagcgc tggccgcgcg gtgacgatga gcccgaaggc
    12181 ccctggcgcc gttcgatcaa ggccaaggca ctggacctgc tgcggggcct tttgccggct
    12241 tcgactctca gtcatgtcgg gatctacgcc tcgggccagg cctgggagca gctgctgttc
    12301 cggatgatgg catcgccgtt accggaggcc cgtcgcttcg gcgggatgat tctcgacgag
    12361 ctgaacaagg tgatccccag tttcgtttcc cgggtaagcc gtcccgatcg cggcggcgag
    12421 tggatttcct ttctcgagca gcgccgccag gcgaccgaag actgggtcga aaggctcggc
    12481 ctcacagacc gcgacagcga agacagcgga ccgacggttg agcttggcca tgttcacggc
    12541 accgaagacg acctgctcgc ggcctgcctt tacgaggagt ccgacgctcc cgaatcgcgg
    12601 attcgggaac ggatcgcccg gcttgattcc cgccagcgcg aagaactcat ggcggcgatg
    12661 atcggcgagc gccgcaaccg tcgccaccgc cccggtcgcg ggttcgagtc ggtctcgtac
    12721 cgcttcgaga tcgtctccga ctacggcgcc ttccgggatc ttcagcgcca tcgcatgctg
    12781 acctgccagt ggcagaccct gactccggat ctcggggccg gggtaccggc cgaagtggtg
    12841 gaagccgggg tcggcggtgc ctacgaagag gcgctcgaga tctcgcgaaa cgagtacgag
    12901 cgactgcgcg acgccggcca tcccgaggcc gccccttacg cgctctgcct cggctaccgg
    12961 atccgttaca tccttgactt gaacgccagg gaggcgatgc aactgatcga gctccgatca
    13021 ggcagggaag gccacccgac ctaccgggcg gtcgcccagg caatgcatga cgaaatcgcc
    13081 gaagtccatc ccgccatcgc cggtgcgatg aagcacctcg acacctcgat ggagccccgc
    13141 ctcgagcgca tgctctccga gatccggacc caccacaagc aactcgccgc cgcccagacc
    13201 tccaaggccc cgcccggctt ctccgaactc ggctgatccg gccagcggcc aaaccggccg
    13261 cgagatccga ctttttcgtc acgatggcga agaactcacg cctcaccgtc cccggccggt
    13321 gtgcccgatt ccctaacctg tcccgatgct ctccgcccgg attcccccgg cgcttccctg
    13381 cgcgctcgcc ttcctgctgc tggcgatgat ggtcgtattc gcagcctgtt cttccgaccc
    13441 cgatcagtcc gaggcgaagc cggcccgctg gcagccgaaa ccgacgaccg cagcctggca
    13501 gttccagctc cagggcaaga tcgacacctc gatcgacgcg ccggtattcg aggtcgacgg
    13561 cttcaacgtg ccgaagtcga ccgtgaccag gcttcactcg atgggtcgca aggtgatctg
    13621 ttacatcgac gtcggcagct gggagaatta ccgccccgac gcgaaacggt tccccgggtc
    13681 ggttatcggc aggaagtacg agggataccc gaacgagcgc tggctcgaca tccgccgcta
    13741 tcggaagttc gcggggccga tcaagtcaag gatccggatg tgcgcccgca agggattcga
    13801 cgggcttgag ccggacaaca tcaacggctg ggagaacccg accggattcc cgctgaccgc
    13861 ccgggaccag cttcgattca accggtggat cgcgaaggtc gcgcataaca acggccttgc
    13921 cgtcggcctc aaaaacgacg gacggcaggc gcgcaagctg gtcggcgcct tcgatttcgc
    13981 cgtggtcgag caatgcttcc agtacagcga gtgcggccag tttctgcctt tcatccgtcg
    14041 tggcaaggcc gtttactcgg tcgaatacga acggcccaat tccgcctttt gcgctcgtgc
    14101 agagacgctt gggtttagtt cgattggcaa ggagtacgac ctcttcgcac ggccctggcg
    14161 cccctgcgcc tagcggtcac gccgcgggat cgggtgagca tcgccgggac ggacgcacgc
    14221 gcggcattca gccgcgctgg tcctggagtg tcccggacga cgctcacccg accccgcggc
    14281 tgcgcctacc cggcggattt tcgcgggagg gctcagtcgg tcaggtcgag gaggctggag
    14341 atgttgtggt tgggggtgac tcgggtttcg gggttgtggc tttcgttgcc ggcgacgagg
    14401 attccttcga ggccggcctt ctgggcgccg ccgacatcgg tgtcgaggcg gtccccgatc
    14461 atcgccaccc gggccccttc cggcaggccg agccgccgga cgccgagatc gaagatcggg
    14521 cgttccggct tgcccgccac caccgcggtc cggcccgagg ccgcttcgac cgcggcgacg
    14581 attgaacccg tcccgggcca gaggccatcc ggcatcggca tggtcgggtc gcggccgatc
    14641 gtcgcgagga aggcgccggc ccgcgccgcc atcgaagcgg ccttcagttc gtcgtaatcg
    14701 aagcggggat ggccggtcac cagcaccgcc tccgcctcct gccagcgttc ggcaggaagc
    14761 agctggaaac ctgcgccttc gacctgctcc agaaatccgc ttgtaccgat cgcatagacc
    14821 ttgccggcct cgccaatctc cttccgggtc agctccagca gggtgtcgcc cgcagtcacc
    14881 acccgatctt cgtcggcttc gattccctgc tcgcgcagaa tcctcgcctg ctcggccggc
    14941 gtcacccggg ggctgttcgt gacgaagacg aacggcttgc ccgcggcagc cagcgcggca
    15001 atggccttcg ccgctccttc gatcggctcg tgaccgagcc agacgacccc gtcgaggtcg
    15061 atcaggaagc cgtcgtatga gtcggccagg gccacgagcc aacaatagag ttgcggcatg
    15121 agtccggtcg gcggcgatct cacaaaacct gcctccgctt cgtctcaggg tcagtgaccg
    15181 gaaatcgcac caacaccgag gggccgctcc cgttcgagac ggcccttgag gatcacgggc
    15241 cgaggctgca tgcctggctg ctcgcgcgag tcggaccctc ccgggccgac gatgtctttc
    15301 aggagacgat gctcgcggcg ctggccgcct gggacagggt cccggttcag tcggtgcgcg
    15361 cgtggctctt cacgatcgcc cggaacaagg cgattgacga gcagcgccgg gatgcgaaca
    15421 ggccccggcc ggaaggcgag gttgacagtt ggccttcggc cgaggttgcc gagcgcctgg
    15481 aggatccggt ctgggatcaa gtgcgggagc tgccggagaa acagcggatc gcggtgaccc
    15541 ttcgctttcg cggtgatctg aaccaccgtg aaatcgggac cgtgatggag atctccgaag
    15601 cggccgcccg ccgcaacgtg ttcgaaggcg tcaagcgcct gcgggaaagg atcgaagatg
    15661 agtgagatcg aagcgagact ggccgccggc gaagtggccg ggaccggaag caactgggag
    15721 gagatccgga ccagcctgct tcggcaggca gaccgggagg gtgccattga cctcgcctac
    15781 gagcggcatg acacaccgtt cgggacgatg gtgatcgtcg cgggggaagg gggcatcc
//
LOCUS       DAOQKN010000036        15611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_1741_length_15611_cov_6.888339, whole genome shotgun sequence.
ACCESSION   DAOQKN010000036 DAOQKN010000000
VERSION     DAOQKN010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 15611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15611
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1741_length_15611_cov_6.888339"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="PLJ64_04005"
     CDS             <1..863
                     /locus_tag="PLJ64_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92687.1"
                     /translation="SQVANEPEHKMLFDVRGRRKRVIQVIYVILAVIMAASLIVIGLP
                     GGINPFNNANSGVNSDYAKANVERADKLQKQLQAQPANEKLAVELIRARLSAGQSLSE
                     TDASGQIVTTDEAADQYDMAASTWTRYLKMTDNNPDPEVAQLMATILFQLSQGSTVAQ
                     FQANIEDAATAQQFVADNAVKEQKKGGVSAWQQLTTLFLYQLYAQDYAAAEKTKQQAL
                     DATDDKGDQQQINQTYKSTKKDAQRVGKLIDQAIKQARKNGGKSLENPLGSLGSDNSI
                     DSGSGSTATP"
     gene            952..1024
                     /locus_tag="PLJ64_04010"
     tRNA            952..1024
                     /locus_tag="PLJ64_04010"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:985..987,aa:Lys,seq:ctt)
     gene            1070..1519
                     /locus_tag="PLJ64_04015"
     CDS             1070..1519
                     /locus_tag="PLJ64_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92688.1"
                     /translation="MSNRELSLKVTGASLPAPAIRDGSNRGRFLVAAAGFVVYETLFI
                     WLLGDRVYDYPYFSPMLWGWVALAAAGFFALGFFSRSWWSSLLLAAPLLVAFYFVYVV
                     WTTNYPNGGSLTVNTNFPEIWLAYSAIFVPVWAAGVLIAKGRGRGSK"
     gene            1530..2270
                     /locus_tag="PLJ64_04020"
     CDS             1530..2270
                     /locus_tag="PLJ64_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92689.1"
                     /translation="MGKYLAGMLLTVFGLFALAPNAGAAEIPPSEECVATAKKLLGEY
                     PDVENFTEEETRKADRKFVNDLADSGCISDSRPLLQKVVLKPNSEKCVTAARGAGAFW
                     GATTKRFRPLNQRWRKQGRPLQRRINRIQRRIRQLREAGASRERLNQLVRIRRPLGRK
                     LQRIQRDLQREARLIVKPMMHESFLVTSELIALRCMPRNLLGESDPGPAERVVRKNID
                     VVLFSLAYLTEATGGEVVFNLYFPTVGR"
     gene            2291..4048
                     /locus_tag="PLJ64_04025"
     CDS             2291..4048
                     /locus_tag="PLJ64_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92690.1"
                     /translation="MGRIRYLLTGFVALAFLGTGFGGTANALTPPAAPSSIAFSFGDR
                     IVRIAADGSGRQSLTRTGPFPDGDLAGRTGDAAPQLSPDGAQLIFTRSLPRKDYGVRR
                     SIMLANADGSGARVILRGNRKVDFSAPAWMPDGKSVAVARSIDRPNGTARSVVIASLN
                     GKSVKTVFTLPLHRRGDLKQDLATYREPIDIGVSPDGKRLLITVSDGYSYDRKWLVLV
                     NLNNGKQRMIGKNTRSGSFSPDGSRFAYVSSTGNSGETCIETDETSCVIQGDLFVQNL
                     DGSGKRRLTRSRAAEENPDWSPDGKRIAFDANYNLPGSGAASEVYAIAPNGTCLSWLT
                     NGSPASVEPDWAPEAGPSDLLCGIEPRETVISSDWIPTKEEGAEISVWAGPVLGNRLV
                     SEAVLTGPFRATVYDDCAVFNARACAEPAKYEIGGLSTCYAGLYLGIVVGEAKRSDFA
                     HRRGVAFLREKRRTRRGNLVRSVTVFTGDTMLAIRGRGNASFADLEKQIDGLRPATAS
                     VSAGRLPQLYLPKGMPNTVKFVAGMVGRIGVRRTSIRLEADPKDIRTLVRFSSRLNLL
                     GPIKTKTCTKEETDPFAGY"
     gene            complement(4071..5330)
                     /gene="arsJ"
                     /locus_tag="PLJ64_04030"
     CDS             complement(4071..5330)
                     /gene="arsJ"
                     /locus_tag="PLJ64_04030"
                     /inference="COORDINATES: protein motif:HMM:NF033734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="organoarsenical effux MFS transporter ArsJ"
                     /protein_id="HNC92691.1"
                     /translation="MAGTARREGDLRNYVLVTAAYWADTLADGASRILVLFFFYQLGY
                     SALEVATLFLFYEFFGIVTNLVGGWIASRLGLKATLFSGLTIQIVALGMLGFAPDEWL
                     VVPYVMVSQALSGIAKDLTKMSSKSAVKLVVPEDSESSLFKWVAILTGSKNALKGVGF
                     FLGGLLLTLIGFQASMLVLLGIVATALITTASMMRGSLGYADMGAKFRHMFSNTREIN
                     VLAAARLFLFASRDVWFVVALPVYLRTVLDWSFWQAGGFLAIWVIGYGIVQASAPGMF
                     ARRSAETGATPTGRSAAKLAFLLAAFPAAIAASLAANLDPTLVLVTGLILFGIVFALN
                     SAVHSYLVLAYTDDESVAKNVGFYYMANAGGRLIGTILSGAVYQWLGLEGCLWFSVAF
                     VLTAATISLRLPSHNPKGAGAMPAAAG"
     gene            complement(5334..6359)
                     /locus_tag="PLJ64_04035"
     CDS             complement(5334..6359)
                     /locus_tag="PLJ64_04035"
                     /inference="COORDINATES: protein motif:HMM:NF033735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsJ-associated glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HNC92692.1"
                     /translation="MTDKLRIGINGFGRMGRLALRAGWDTEELEFVQINEIAGGPESA
                     AHLLEFDSVQGRWRREGIEWLEDSIRVAGKELAYTSGSDPGGVDWGKDEVDLVIEATG
                     KFRTPELLAPYFGRGVKRVLVAAPVKDDALNIVYGVNDSDYDPDRDRIVTAASCTTNC
                     LAPIVKVVHEGIGIRHGSITTLHDLTNTQVVVDAHHKDLRRARSAGTSLIPTSTGSAT
                     AIGMIFPDLVGRLDGLAVRVPLLNSSITDCCFEVDRATSVEEINDLLRTAASEELGGI
                     LGFETRPLVSVDFKGDPRSGIVDGTSTRVIDQTQVKILAFYDNEWGYANRMIDIARMI
                     ARSSGGD"
     gene            6446..6799
                     /locus_tag="PLJ64_04040"
     CDS             6446..6799
                     /locus_tag="PLJ64_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNC92693.1"
                     /translation="MSSALTLSPKQKSPNGQPCCEPVVYPDVERAEAERMAAIAKALG
                     DPIRLTLVDVLRKHAGKVCVCELVPLFDVSQSTLSHHLKKLRDAGIVDSERQGLWAYY
                     YVNPEELDELSAWLS"
     gene            6787..7665
                     /gene="arsM"
                     /locus_tag="PLJ64_04045"
     CDS             6787..7665
                     /gene="arsM"
                     /locus_tag="PLJ64_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HNC92694.1"
                     /translation="MAELEQVNDCCTPEAQASCCEAEARDECCGEEQGCGCQEGSERI
                     ESELREIVRERYAAAAVDAGSRDASCCANDTVITEEQRAVFGSNLYEEGDRSELPEAA
                     QLASLGCGNPTAVADLEEGQTVLDLGSGGGIDVLLSARRVGPTGKAFGLDMTDEMLEL
                     ARSNQKEAGVENVEFLKGEIEAVPLPDDSVDVVISNCVINLSGDKARVLREAARVLRP
                     GGSFAVSDVIADPDMTEETRADMREWTGCIAGALTREEFESNLAAAGFEDIQIEATHR
                     VHDQAESAIIRATLPA"
     gene            7662..8036
                     /locus_tag="PLJ64_04050"
     CDS             7662..8036
                     /locus_tag="PLJ64_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92695.1"
                     /translation="MSTDEIQLNDPQTLYERWEGAQWNPFTVPLEWDIEQWAGMGDRD
                     RGLVYWVLSSLMVAEERITTKFSGLVGAHGSEEEASFLATQQVDEARHIGYGVWFLRE
                     SVRESPDVAPEAIRSMLRTLLP"
     gene            8037..8186
                     /locus_tag="PLJ64_04055"
     CDS             8037..8186
                     /locus_tag="PLJ64_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92696.1"
                     /translation="MAESLSPSPGDDGPDLDALGVSGEDIRNFALGGLTRRLEIIGVP
                     LETVF"
     gene            8268..9440
                     /gene="chrA"
                     /locus_tag="PLJ64_04060"
     CDS             8268..9440
                     /gene="chrA"
                     /locus_tag="PLJ64_04060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HNC92697.1"
                     /translation="MPVDQDSRAAIFWRFLKFGLLAWGGPAAQIEMIRHECVEQEKWI
                     SAETFKKQLAVYQILPGPEAHELCVYLGRVRGGKLGGFLAGLGFMLPGFILMLGLSVL
                     YVKASLAGHLDQVFYGLTAAVGALVARAVVRLSKNFLTDIPLVIIAALAFGLTLGISA
                     SFVLVLLGAGIAYELWTNRHGWTRRTNSFGFSPLVLVLAATGAITLSLTAEIFFEGLK
                     AGLLTFGGAFTVIPYLQESAVNNHHWLTDSQFVDGLAMSGVLPAPLIIFSTFVGYIAG
                     GMTGALVMTLGIFLPAFIFPIFFHRQLVAVAENARLHPFLLGVAAGVIGLIAAVTVSI
                     LQTSVVDVWTGLIALGAFLALQKWHSKLTVVYVMAAAGLIGVPLQGNRRLSSDRDP"
     gene            complement(9423..11324)
                     /locus_tag="PLJ64_04065"
     CDS             complement(9423..11324)
                     /locus_tag="PLJ64_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HNC92698.1"
                     /translation="MSSSLSSGRVSPKATAGAAALTLGALGVVFGDIGTSPLYAFQTV
                     FQADHHAVKPNDTDVFGVISLVFWSITMIVSVKFVTFIMRADNDGEGGIMALIALIQG
                     ASLGNRFVKAGLIGAGLFGVALFYGDGMITPAISVMSAVEGVEVAAPSLSDYVLPMTI
                     GVLVILFAAQRFGTHVIGRAFGPVMVLWFIVIGAAGIGQIAADPEILKALNPAYGVDF
                     FSAHLRIAFISLGAIVLTVTGAEALYADMGHFGRPPIRRAWFLLVFPALTLNYLGQGS
                     LVLQDPSSVSSPFFLLFPDWSHIPMVVLATIATVIASQAVISGAFSVTKQAIQLGYLP
                     RLLIGQIYMPAVNWALFFAVVILVVGFGSSAALASAYGIAVVGTLTIDAILFLVVVRK
                     LWQKPLWMAICFGVVFLTIDFTFLAANLTKVEHGGWFPLSIGLIILTVILTWDRGYRA
                     TAAKRAELEGSLTGLVDEINRKDPGPIRVRGTAVYLNARTNTAPLAFRSALERDGIVH
                     DHVVVLSLVTARQPYVEDKDRIVIDDLGYDYDGISHITATVGFMDVPDVPYLLELANP
                     QVPDGPLDFSKAYYYLSNVLIRPYGDTRLHRLLKHLYITMARNASSPVDHFRLPAERT
                     VIESSGISI"
     gene            11407..12327
                     /locus_tag="PLJ64_04070"
     CDS             11407..12327
                     /locus_tag="PLJ64_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92699.1"
                     /translation="MRNVAGFFTVPKNTGLDFPPVTVDLDVVQIQGEIALTKDGSGHF
                     DEATGAMNMDLSLSLTLGVDDLEALSQQVGIPLGTGALGCKISPLEVSFSTAKGWPHA
                     GSSFTDKATPADGALAGAWRYKPAITAVEGDQSVCNIIGGFLKDVGGIWLGNSTTPLA
                     DMPAATDPMPAKANCTELGKTGTFPNCVDPVCPEGQLGTYPDCHDPAPFAVSKVALSP
                     TKANIKKNKTVKLTLKVTNSGEVSGSATVSLKSSNKQVTLPKSIKVTVPAGKTASKTI
                     TVKAAKKAKGKATITATASGKKGTATLTVK"
     gene            12479..12551
                     /locus_tag="PLJ64_04075"
     tRNA            12479..12551
                     /locus_tag="PLJ64_04075"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12512..12514,aa:Phe,seq:gaa)
     gene            complement(12651..13448)
                     /locus_tag="PLJ64_04080"
     CDS             complement(12651..13448)
                     /locus_tag="PLJ64_04080"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HNC92700.1"
                     /translation="MRTIEGSSGRIVVHEWIPVGEPTYVVLLAHGYAEHALRHAGLAE
                     KFEADGAAVYAPDHRGHGRSEGEPALVDEAQPMIDDLAAVYDLAREEHPGLPILLFGH
                     SMGGLLATLFIQEAERPVDAVVLSSPLLGGNPALVALLDLEEIPEIPIDPTVISRDEN
                     EQQAYLDDPMIYHGPFRRETLEGLVAGLDRARSGDGFGELPLLWIHGEGDMLVPYDLT
                     AETMEILSGPGTEAKSYPEARHELFHETNREDVYADLFDFVNRNLET"
     gene            complement(13491..>15611)
                     /locus_tag="PLJ64_04085"
     CDS             complement(13491..>15611)
                     /locus_tag="PLJ64_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNC92701.1"
                     /translation="LNRGGIEQLYSHQAEAFEAAAEGNVILTTATASGKSLAFNLPPL
                     NAIAADPKARALYLYPTKALAQDQARALARLGSPNLRLAIYDGDTPRDERPAIRRQSN
                     LVLTNPDMLHVGIMPHHRGWGDFLANLDWVVVDEAHTYRGVFGSHVANVLRRLRRLAR
                     AYGREPRFVMASATIANPAELAERLTGSPARLIDEDGAPRAGREIRIWNPPLVNPQVG
                     ARRSALAEAADLMADLVSHEVRTICFLKSRKGIELIRKFAADRLTEKGRPDLAERIAP
                     YRAGYTPAQRREIERKLVSGELLGVASTDALELGIDIGGLDAAICVTFPGTVAGLKQM
                     WGRAGRRERGLAVYIAGPDGLDQYFCRHPDEFLERPVEAAILDHLSPEIRDQHLIAAA
                     YELPLTSEDEEFFGSGLEDAGQALVLRGELRKAGKGLIPRRAGFAASDVSLRSSSAGD
                     VAVIDRDSGEVIGTVETGRAFSTIHPGAVYMHLGASWEVERLDPYEGEAIVHRFDEEW
                     YTRPKTETEIFINRVLEEKEVCGVKLSFGEISVTDQVMAYQRILSADGEAFDLVDLDL
                     PEQEFATRSLWYEVPEGFIGGMALNEMLGALHATEHGQIAVLPLIAMCDRWDIGGLST
                     NLHMQTGTPTIFIYDGHPGGIGLTKRAFELFPRLVGDAHRLIAECPCDSGCPSCVQSP
                     KCGNLNEPLFKAGSISLLEAMASA"
BASE COUNT         2909 a         4976 c         5075 g         2651 t
ORIGIN      
        1 ctagccaagt ggccaacgaa cccgaacaca agatgctttt cgacgtgcgc ggtcggcgca
       61 agcgggtgat tcaggtcatt tacgtgatcc tcgccgtgat catggcggcc agcctgatcg
      121 tgatcggact gcccggcggc atcaacccgt tcaacaacgc caacagcggg gtcaacagtg
      181 attacgccaa ggcgaacgtc gagcgggccg acaaactcca gaagcagctc caggcccagc
      241 ccgccaacga gaagctcgcg gttgaattga tccgggcccg gctctcggcc ggccagtcgc
      301 tgtcggagac cgacgccagc ggccagatcg tgaccaccga cgaagccgcc gaccagtacg
      361 acatggcggc ttcaacctgg accaggtacc tcaagatgac ggacaacaat ccggatcccg
      421 aggtcgccca gctgatggcc acgatcctct tccagctttc ccagggttcg accgtcgccc
      481 agttccaggc gaacatcgag gacgcggcca cggcgcagca attcgtggcg gacaacgcgg
      541 tcaaggaaca gaagaagggc ggagtcagcg cctggcagca gctcaccacc ctcttcctct
      601 accagctata cgcccaggat tacgcggccg ccgagaagac gaagcagcag gctctcgacg
      661 cgaccgatga caagggcgac cagcagcaga tcaaccagac ttacaagtcg accaagaagg
      721 acgcgcagcg ggtcggcaag ctaattgacc aggcgatcaa gcaggcccgg aagaacggcg
      781 gcaagtcgct cgagaacccg ctcggcagcc tcggctccga caactcgatc gacagcggct
      841 cgggcagcac cgccactccc tgatccaccc gggacctgag agcgggcggg ccacagaatc
      901 ggctcgcccg ctctataatc cgcgtccggc ttttcaagga agccgcaccc cgggccgtta
      961 gctcagttgg tagagcagga gactcttaat ctcaaggtcg taggttcgat ccctacacgg
     1021 cccataggga aacgattcga caggggatat gtcggcagga ggcatgacag tgagtaacag
     1081 ggaactcagc ttgaaggtca cgggggcgtc gcttccggca ccggcaatcc gggacggcag
     1141 caatcgaggc cgcttcctgg tcgcggccgc cggcttcgtg gtttacgaga cgctcttcat
     1201 ctggctcctc ggcgaccggg tctatgacta cccgtacttc agccccatgc tttggggctg
     1261 ggtcgctctc gccgcagccg gtttcttcgc cctgggtttc ttctcgaggt cgtggtggtc
     1321 ctcgctcctt ctggcggcac cgttgctcgt cgccttctac ttcgtgtacg tcgtctggac
     1381 cacgaactac ccaaacgggg gcagcctcac cgtcaacacc aacttccccg agatctggct
     1441 cgcctactcg gccatcttcg tcccggtctg ggctgcaggg gtgttgattg caaagggccg
     1501 gggtcgaggg tcgaaataga atgaagcgca tggggaaata cctggcaggg atgctgttga
     1561 cggttttcgg gctgttcgcg cttgctccga acgcgggagc ggccgaaatt ccgccgagcg
     1621 aggagtgcgt cgccaccgcc aagaagctgc tcggcgagta tccggacgtc gagaatttca
     1681 ccgaggagga gaccagaaag gccgaccgga agttcgtaaa cgatctcgcc gattcaggct
     1741 gcatttcaga ttccaggcca ctccttcaga aggttgtgct caagccgaac agcgagaagt
     1801 gcgtaacggc tgcgcgaggg gccggagcgt tctggggagc aaccacgaag aggttccgac
     1861 cgctcaacca gaggtggcgg aaacagggcc gtcccctcca gcgacgcatc aaccgaattc
     1921 agcggcggat ccgccagctc cgggaagccg gggcctcccg ggagcgactc aatcagctgg
     1981 ttcgaatccg gagacctctc ggcaggaagc tccaaaggat ccagagagat ctccagcggg
     2041 aagcccgcct gatcgtgaag ccgatgatgc atgagtcctt cctcgtcacc agcgaactga
     2101 tcgccctccg ctgcatgccc cggaaccttc tcggtgaaag cgatccgggg ccggcggaaa
     2161 gggtggtcag gaagaacatc gacgtggtcc tgttttccct ggcctacctg accgaagcga
     2221 ccggcggcga ggtcgtgttc aacctctact tcccgaccgt aggtcgctga agcagagggg
     2281 aaggggaagg atgggccgga ttcggtactt gctcaccggt ttcgtcgcgc tggcatttct
     2341 cggaaccgga tttggcggta ccgccaatgc gctgactccg cccgctgctc cgagcagcat
     2401 cgctttcagc ttcggtgacc ggatcgttcg catcgccgcc gatggttcgg gacggcagtc
     2461 gctgaccagg accggcccct tcccggatgg cgacctcgca ggccgtaccg gtgacgctgc
     2521 tccccagctt tcgcccgatg gcgcccagct cattttcacc cggtctcttc cgcgaaagga
     2581 ctacggcgtc cgcaggtcga tcatgctggc caatgccgat ggcagcggag cccgcgtgat
     2641 ccttcgcggc aaccgcaagg tcgacttttc cgccccagcc tggatgcccg acggcaagtc
     2701 cgtcgcggtg gctcgcagca tcgaccggcc caacggcaca gcgcgctcgg tcgtgatcgc
     2761 cagcctcaac ggcaagtcgg tgaaaacggt cttcacgctg ccgctccacc gccggggaga
     2821 cctgaagcag gacctcgcca cctaccgcga accgatcgac atcggtgttt cccccgacgg
     2881 aaagcggttg ctgatcacgg tgagcgacgg ctacagctac gaccggaagt ggctcgtgct
     2941 ggtcaacctg aacaacggca agcagcgcat gatcggcaag aacacgcggt ccggctcctt
     3001 ctcacccgac gggagccgct tcgcctatgt ctcgtcgacg ggcaacagcg gtgaaacctg
     3061 catcgaaacc gatgaaacga gctgcgtcat ccagggtgac ctgtttgttc agaacctcga
     3121 cggcagcggc aagcggaggc tgacccggag tcgcgccgcg gaggaaaacc cggactggtc
     3181 ccccgacggc aagcggatcg ctttcgacgc caactacaac ctccccggat cgggcgcggc
     3241 ctccgaggtc tacgcgatcg ctccgaacgg gacctgcctt tcgtggctca ccaacggttc
     3301 gccggcgtca gtcgaaccgg actgggctcc cgaagccggc ccttcggatc tcctttgcgg
     3361 aatcgagcca agggaaacgg tgatttccag tgactggatc cccaccaagg aggagggtgc
     3421 cgagatcagc gtctgggccg gtcccgtgct gggcaatcgc ctggtctccg aggccgtcct
     3481 gacgggtccc ttccgcgcca ctgtgtacga cgattgcgcg gtcttcaacg ccagggcctg
     3541 cgccgagcct gcgaagtatg aaatcggcgg gctttccacc tgctacgcgg gtctttacct
     3601 cggcatcgtg gtcggcgaag cgaagcgatc cgacttcgcc catcgccggg gcgtcgcctt
     3661 ccttcgtgaa aagcggcgca ctaggcgcgg gaatctggtc cgtagcgtca cggtcttcac
     3721 cggtgacacc atgcttgcga ttcgcggccg agggaacgcc agcttcgccg atctcgagaa
     3781 gcagatcgac gggcttcgac cggccaccgc ttccgtgtcg gccgggcgtt tgccgcagct
     3841 ctacctgccg aagggaatgc cgaacacggt caagtttgtg gccgggatgg tcggccggat
     3901 cggagtcagg cgaacctcga tcaggctgga ggccgacccg aaggacatcc ggaccctggt
     3961 cagattctcg agccgcctga acctgctcgg cccgatcaag accaagacct gcaccaaaga
     4021 ggaaacagac cccttcgccg gttattgaac gggcggaccg ggatgccttg ctagcccgcg
     4081 gcggccggca ttgccccggc gcctttcggg ttgtgcgatg gaagccgcag cgagatcgtc
     4141 gcggcggtga gcacgaacgc gaccgaaaac cagaggcatc cttccaggcc gagccactga
     4201 tagacggctc cggagaggat cgttccgatc agcctgccgc cagcgtttgc catgtaatag
     4261 aagcccacat tcttggcgac cgattcgtca tcggtgtatg cgagcacgag gtaagagtgc
     4321 acggctgaat tgagcgcgaa gacgatcccg aacaggatca ggccggtgac cagaaccagg
     4381 gtcggatcta gattcgcggc aagcgacgca gcgatcgccg cagggaaggc ggcgagaagg
     4441 aaggcaagtt tcgccgcgga ccggccggtc ggagtggctc ccgtctccgc gcttctcctg
     4501 gcgaacattc cgggagcgct cgcctggacg atcccgtaac cgatcaccca gattgccagg
     4561 aagccaccgg cctgccagaa actccagtcg aggaccgtcc tcaggtagac cgggagagcg
     4621 acgacgaacc agacatcccg ggaggcgaag aggaagaggc gggcagccgc gaggacgttg
     4681 atctcccggg tgttggaaaa catgtgacgg aacttcgccc ccatgtccgc atagccaaga
     4741 ctgccccgca tcatcgacgc ggtggtaatc agggcggtgg cgacgatccc gagcaatacc
     4801 agcatcgagg cctggaaccc gatcagggtg agcagcagcc cgccgaggaa gaagccgacg
     4861 cccttgaggg cattcttgga accggtgagg atcgccaccc atttgaagag agatgattcg
     4921 gagtcctctg ggacgacgag cttgaccgca ctcttcgagc tcatcttggt caggtccttg
     4981 gcgatgccgg agagcgcctg ggagaccatc acgtacggaa cgaccagcca ctcgtcgggc
     5041 gcgaaaccga gcatcccgag cgcgacgatc tggatggtca gcccggagaa aagcgtcgcc
     5101 ttcaatccga gccgggaggc aatccatcct ccgaccaggt tggtgacgat cccgaagaac
     5161 tcgtagaaga ggaacagcgt cgcgacttcc agcgcgctgt aaccgagctg gtagaagaag
     5221 aacaggacga ggatgcggct ggcaccgtcg gcgagcgtgt cggcccagta ggcggccgtg
     5281 accaggacgt agttcctgag atcaccttct cttcgcgcgg tgccggccat cgctcagtct
     5341 ccgccggagc ttcgggcgat catcctggcg atgtcgatca tccggttcgc gtatccccac
     5401 tcgttgtcgt agaaggcgag gatcttgacc tgggtctgat cgatcacccg ggtcgaggtt
     5461 ccgtccacga tgccggaccg ggggtcaccc ttgaagtcga cggaaaccag tggcctcgtc
     5521 tcgaagccga ggattccccc aagctcctcg ctggcggccg tccggagcag gtcattgatt
     5581 tcttcaacgg aagttgcacg gtcgacctcg aagcagcagt cggtgatcga ggagttgagg
     5641 agcggcactc gcaccgccag cccgtccagt cgcccgacga gatcggggaa gatcattccg
     5701 atcgcggttg ccgatccggt tgaggtcggg atcagcgaag taccggccga cctcgcgcgc
     5761 cggaggtcct tgtggtgggc gtcgaccacg acctgcgtgt tggtcaggtc gtggagggtg
     5821 gtaatcgatc cgtgccggat gccgatgccc tcgtggacaa ccttgacgat cggggccagg
     5881 cagttcgtcg tgcaggaggc ggcggtgacg atgcgatccc tgtcggggtc gtaatcggag
     5941 tcattcacgc cgtagacgat gttcagcgcg tcatctttga ccggtgccgc gacaaggact
     6001 ctcttcacgc cccggccgaa gtagggagcc aggagttccg gtgtgcggaa ctttccggtg
     6061 gcctcgatca ccagatcaac ttcgtccttg ccccagtcaa ctcccccggg atcgctcccc
     6121 gaggtgtagg caagttcctt gccggcgacc cggatcgagt cttccagcca ctcgattcct
     6181 tcccggcgcc agcgtccctg caccgagtcg aactcaagca ggtgcgccgc agactcgggg
     6241 cccccggcga tctcgttgat ctggacgaat tcgagttcct cggtgtccca gccggctcgc
     6301 agagcgagcc tccccatcct tccgaagccg ttgattccaa ttctcagctt gtctgtcatc
     6361 cgtcgccctc ccgggcctcg tttgatctat cgatgactat cgtatctatt gacaaacgtc
     6421 aattgaactg atttggtgaa tatcgatgag ctccgcactg accctcagcc ccaaacagaa
     6481 gagtccgaac ggccagcctt gttgcgagcc ggtcgtctac ccggacgtcg aacgcgccga
     6541 agccgaacga atggccgcga tcgcaaaggc acttggcgac ccgatccggc tcacactggt
     6601 cgacgtactt cggaaacacg ccggcaaggt ctgcgtctgc gagctcgtac ccctgttcga
     6661 cgtcagccag tcgacgctct cccatcatct gaagaagctg agggatgccg gaatcgtcga
     6721 ttcagaacgc cagggactgt gggcctatta ctacgtgaac cccgaagagc tggacgagtt
     6781 gagcgcatgg ctgagctaga gcaggtgaac gactgttgca ccccggaggc ccaggcttcc
     6841 tgctgcgaag ccgaagccag ggatgagtgt tgcggtgaag agcagggttg cgggtgccag
     6901 gaaggaagtg aacgcatcga atccgaactc agggagatcg tgcgagagag gtacgcggcg
     6961 gcggcagttg acgccggctc ccgggatgcg tcctgctgcg cgaatgacac ggtcatcacc
     7021 gaagagcagc gggcagtatt cggatcgaac ctctacgaag aaggcgaccg aagcgaactg
     7081 cccgaagctg cacagctcgc ctcgctcggc tgcggaaatc cgactgccgt cgctgatctc
     7141 gaagaaggtc agaccgttct cgatctcgga tccggtggcg gcatcgacgt cctgctctcg
     7201 gcccggcggg tcggcccgac cggcaaggcc ttcggcctcg acatgaccga cgagatgctc
     7261 gaactcgctc gcagcaacca gaaggaagcc ggtgtcgaga acgtcgaatt cctgaagggg
     7321 gagatcgaag cggtacccct ccccgacgac tcggttgacg tggtgatctc caactgcgtg
     7381 atcaaccttt ccggcgacaa agccagggtc ctgagggagg cggcgcgggt ccttcgaccg
     7441 ggcgggagtt ttgccgtctc ggacgtgatc gccgatccgg acatgaccga agagacacgg
     7501 gcggacatga gggaatggac cggctgcata gccggcgccc tgacccgcga agagttcgaa
     7561 tcgaaccttg cggcagccgg cttcgaggac atccagatcg aggcgacaca ccgcgtccac
     7621 gaccaggccg aatcggcgat catccgagct accctgcccg catgagcacc gacgagatcc
     7681 agctgaacga tccccagacc ctctacgaaa ggtgggaagg ggcgcagtgg aatccgttca
     7741 cggtaccgct ggagtgggac atcgagcagt gggccggaat gggggacagg gatcgaggac
     7801 tggtttattg ggtgctctcg tcactgatgg tcgccgagga gagaatcacg accaagttct
     7861 ccggtctggt cggggcccac ggatcggaag aggaagcgag ctttctcgcg acccagcagg
     7921 tcgacgaggc ccgccacatc ggctacggag tctggttcct tcgcgagtcg gtccgggagt
     7981 caccggatgt ggcgcctgag gccatccggt cgatgcttcg gacgctgctg ccttaggtgg
     8041 ccgaatcgct ctcgccgtct cccggagatg atggcccgga cctcgacgct ctcggtgtca
     8101 gtggcgagga catccggaac ttcgccctgg gcggcctgac caggcgcctg gagataatcg
     8161 gcgttccact cgagacggtc ttctagaact gcgggtggtc accggcgtct gcgcgctttc
     8221 tctccgggaa gtaacccgga tgcggccgga acttggtaaa agctgccatg cccgttgacc
     8281 aggattcccg agcagccatc ttctggcgct tcctcaagtt cggtttgctc gcctggggag
     8341 gaccggcggc ccagatcgaa atgatcaggc acgagtgcgt cgagcaggag aagtggatct
     8401 ccgcggagac cttcaagaag cagctcgctg tctaccagat acttcccgga ccggaagccc
     8461 atgagctctg tgtctacctg ggccgggtgc ggggcgggaa gctcggcgga ttcctcgccg
     8521 gtctcggctt catgctgccc ggcttcatcc tcatgttggg gctctcggtc ctctacgtca
     8581 aggccagcct ggccgggcat ctggaccagg tcttctacgg actgacggca gcggtcggcg
     8641 ccctggtggc ccgcgccgtg gtccggctct cgaagaactt cctcaccgac attccgttgg
     8701 tcatcatcgc tgcgctcgcc ttcggactga ccctgggcat tagcgcaagc ttcgtgctcg
     8761 tcctgctcgg cgccggcatc gcgtacgagc tgtggaccaa ccggcatggc tggacccggc
     8821 ggaccaattc gttcgggttc agtcccctgg tgctcgtgct ggcggcgacc ggggcgatca
     8881 ccctttccct gacggccgag atcttcttcg aaggcctgaa ggccgggctg ctgaccttcg
     8941 gcggggcctt cacggtcatt ccctacctcc aggaaagcgc cgtcaacaac catcactggc
     9001 tgactgacag ccagtttgtc gacggactgg cgatgagtgg tgtcctgccg gcaccgttga
     9061 tcattttctc caccttcgtc ggatatatcg ccggcggaat gaccggcgcc ctggtcatga
     9121 ccctgggcat cttcctgccc gccttcatct tcccgatctt ctttcaccgc cagctcgtcg
     9181 cggtcgccga aaatgcccgg cttcatccgt tcctgctggg agtcgctgcg ggggttatcg
     9241 ggctgatcgc cgcggtcacg gtctcgatcc tccagaccag cgtcgtcgat gtctggaccg
     9301 ggctgatcgc cctcggggct ttcctcgccc tccagaagtg gcactcgaag ctgacagtcg
     9361 tctatgtaat ggccgccgcc gggctgatcg gagtcccgct ccaaggcaac cgtcgtctga
     9421 gctcagatcg agatccctga actttcgatc acggttcttt cggcggggag gcggaagtgg
     9481 tctaccgggc ttgaggcgtt tcgggccatc gttatgtaga ggtgcttgag gaggcggtgg
     9541 agcctggtgt caccgtacgg gcggatcaga acgttggaga ggtagtagta ggccttgctg
     9601 aaatcgagcg ggccgtcggg gacctggggg ttggcgagct ccagcagata gggaacgtcg
     9661 ggaacatcca tgaaaccgac cgtggcggtg atgtgggaga tcccgtcgta gtcgtagccg
     9721 aggtcatcga tcacgatccg gtccttgtcc tcgacatagg gctggcgggc ggtgaccagg
     9781 gagagcacca ccacgtggtc gtggacgatc ccgtcgcgct cgagggccga gcggaaggcc
     9841 agcggagcgg tgttggttcg ggcgttcagg tagacggcgg tgccccggac ccggatcggc
     9901 cccggatcct tgcggttgat ctcgtccacc agtccggtga gcgaaccttc cagctcggcc
     9961 cgctttgccg cggtcgcccg gtaaccgcga tcccaggtca ggatcacggt caggatgatc
    10021 aggccgatcg agagcgggaa ccagccgcca tgttcgacct tggtcaggtt cgcagcgagg
    10081 aaggtgaagt cgatggtcag gaaaacgacc ccgaaacaga tcgccatcca gagcggtttc
    10141 tgccagagct tcctgaccac gaccaggaag aggatcgcgt cgatcgtcag ggtgccgacc
    10201 acggcgatcc cgtaggcgga ggccagcgcg gccgaggagc cgaaaccgac caccagaatc
    10261 accaccgcga agaacagcgc ccagttgacg gccggcatgt agatctggcc gatcaagagg
    10321 cgcggcaggt accccagctg gatcgcctgc ttggtcaccg agaaagcacc ggagatcacc
    10381 gcctgcgagg cgatcaccgt ggcgatcgtg gcgaggacga ccatcgggat gtgcgaccag
    10441 tcggggaaga gcagaaagaa cgggctggaa accgaggagg ggtcctgcag gaccagcgat
    10501 ccctggccga ggtagttcag ggtcagggcg ggaaagacga gcaagaacca ggcccggcgg
    10561 atcgggggcc ggccgaagtg gcccatgtcg gcgtaaaggg cctcggcacc ggtgaccgtg
    10621 agcacgatcg cccccagcga gatgaaggcg atccgcaggt gggcggaaaa gaagtcgacc
    10681 ccgtaggccg ggttgagggc cttgaggatt tccgggtcgg cagcgatctg gccgatcccg
    10741 gcggccccga tcacgatgaa ccagaggacc atcaccggcc cgaaggcccg gccgatcaca
    10801 tgggtgccga aacgctgggc tgcgaagagg atcaccagca cgccgatcgt catcggcagc
    10861 acgtaatcgg aaagagacgg cgccgcgacc tcgaccccct cgaccgcgga catgaccgag
    10921 atcgccgggg tgatcatgcc gtcgccgtaa aagagagcga ccccgaagag tccggccccg
    10981 atcagccccg ccttcacgaa ccggttgccc aggctggccc cctgaatcag cgcgatcagg
    11041 gccatgatgc cgccctcccc gtcattgtcg gcccgcatga tgaaggtcac gaacttgacc
    11101 gagacgatca tcgtgatcga ccagaagacc agcgagatca cgccgaagac atcggtgtcg
    11161 ttcggtttga ccgcgtggtg gtcagcctgg aaaaccgtct ggaaggcgta aagggggctg
    11221 gtgccgatgt ccccgaagac aacgccgagc gcccccagcg tgagggcggc cgcaccggcc
    11281 gtggctttcg gtgaaaccct gcctgaggaa agggacgaag acatcagccg gggagcctac
    11341 gcctcgcgcc ggtttccggc tcgatcgccc cctctacccg caaccgccaa tccagaaacc
    11401 gattgtttgc gaaatgttgc cggtttcttc accgtgccga agaacaccgg cctggacttc
    11461 ccgccggtca ccgtcgacct cgacgtcgtc cagatccagg gtgaaatcgc cctgaccaag
    11521 gacggctccg gtcatttcga cgaggccacc ggtgcgatga acatggatct cagcctctcg
    11581 ctgaccctcg gcgttgacga cctcgaagct ctctcgcagc aggtcggcat cccgctgggc
    11641 accggcgcgc tcggctgcaa gatctcgccg ctggaagtct ccttctcgac cgcgaagggc
    11701 tggccgcatg cgggcagcag cttcaccgac aaggcaactc cggccgacgg cgccctggca
    11761 ggtgcctggc gctacaagcc ggcgatcacc gctgttgaag gcgaccagtc cgtctgcaac
    11821 atcatcggtg gcttcctcaa ggacgtcggt ggcatctggc tcggcaacag caccacgccg
    11881 ctcgccgaca tgcctgccgc gacggacccg atgccggcaa aggccaactg caccgagctc
    11941 ggcaagacgg gtaccttccc gaactgtgtc gacccggtct gccccgaagg ccagctgggg
    12001 acctaccccg actgccacga tccggctccc ttcgcggtga gcaaggtcgc gctttcgccg
    12061 accaaggcca atatcaagaa gaacaagacc gtcaagctga ccctcaaggt caccaactcc
    12121 ggagaggttt ccggtagcgc gacggtttca ctgaagtcga gcaacaagca ggtcaccctg
    12181 ccgaagtcga tcaaggtgac cgttccggcc ggcaagacgg ccagcaagac catcacggtc
    12241 aaggctgcca agaaggccaa gggcaaggcg accatcaccg ccaccgccag tggcaagaag
    12301 ggcaccgcca ccctgacagt caagtagcga ggacacttct tccggccagg ccggaccgag
    12361 tgaagcgagg gccccggagt gatccggggc cctttccttt gcccgggttt cccggacccg
    12421 tttcggcccc gcgccaagcg tcgctgaacc ggcgattccc gatagcctgt ccttccttgg
    12481 cgaggtagct cagttggtag agcacacgac tgaaaatcgt ggtgtcgccg gttcgatccc
    12541 ggccctcgcc atatgtcacg agtagtcggg cggggtccga cccgccctcc acgagcaacc
    12601 cgcccgaccc actggaagaa atgaatggtg ttgggtcggg ctctgccctg ctaggtctcg
    12661 aggtttcggt tgacgaagtc gaagaggtcg gcgtagacgt cttcgcggtt ggtttcgtgg
    12721 aatagctcgt ggcgggcttc cgggtatgac ttcgcttcgg tgcccgggcc gctcaggatc
    12781 tccatcgttt cggcggtcag gtcgtagggg acgagcatgt ccccctcgcc gtgaatccag
    12841 agcagcggca actcgccgaa accgtcaccg ctccgggccc ggtccagccc ggcgaccagg
    12901 ccttccagcg tttcgcgacg gaaggggccg tggtagatca tcgggtcgtc gagataggcc
    12961 tgctgctcgt tctcgtcacg gctgatcacc gtcgggtcga tcgggatctc cgggatctcc
    13021 tcgaggtcga gcagggcgac cagtgcggga ttgccgccca gcagcgggct ggagaggacg
    13081 accgcatcaa ccgggcgttc cgcctcctgg atgaacaggg tggcgagcag accccccatc
    13141 gaatggccga agagcaggat cggcaggccg gggtgttcct cgcgagccag gtcatagacc
    13201 gcggcgaggt cgtcgatcat gggctgggct tcatcgacca gggccggctc gccctccgag
    13261 cgcccgtggc cgcgatgatc cggcgcgtag accgccgccc cgtccgcctc gaacttctcg
    13321 gccaggccgg catgcctgag cgcgtgttcg gcgtaaccat gggcgagcag caccacatat
    13381 gtcggctccc cgaccggaat ccactcgtgg acgacgatcc tgccgctgct tccctcgatg
    13441 gtgcgcatcc gctctccttt gactttgctg gtgccttctg ggtggccgaa tcaggccgaa
    13501 gccatcgcct ccagcaggct gatcgagccc gccttgaaca gtggctcgtt caggttcccg
    13561 catttggggc tctgaacgca agaggggcaa ccggaatcgc aggggcactc cgcgatcagg
    13621 cggtgggcat cgccgaccag gcgggggaag agctcgaagg cgcgcttggt cagcccgatg
    13681 ccgccggggt ggccgtcata gatgaagatg gtcggggtgc cggtctgcat gtggaggttg
    13741 gtcgaaaggc cgccgatgtc ccagcggtca cacatcgcga tcagcggcag caccgcgatc
    13801 tgcccgtgct cggtcgcgtg gagggcgccg agcatctcgt tcagggccat gccgccgatg
    13861 aaaccctcag gtacctcgta ccagaggctc cgggtcgcga actcctgctc gggcaggtcg
    13921 agatcaacca ggtcaaacgc ctcgccgtca gcgctgagga tgcgctggta ggccattacc
    13981 tggtcggtga ccgagatctc gccgaagctc agcttcaccc cgcagacttc cttctcctcg
    14041 agcacccggt tgatgaagat ctcggtttcg gtcttcggcc gggtgtacca ctcctcatcg
    14101 aaacggtgga cgatcgcctc gccctcgtac ggatcgagcc gctccacctc ccaggaagcg
    14161 ccgaggtgca tgtagaccgc gccgggatgg atggtgctga aggcgcgacc ggtctccacc
    14221 gtgccgatca cctcgccgga atcacggtcg atcaccgcga cgtccccggc cgaggaggag
    14281 cggagcgaaa cgtcggatgc ggcgaaaccg gcccgcctcg gaatcagccc cttgccggcc
    14341 ttgcgcaact cgcccctgag gaccagggcc tggccggcgt cctccaggcc ggagccgaag
    14401 aactcctcgt cctcggaggt gagcggcagt tcgtaggcgg cggcgatcag gtgctggtca
    14461 cggatctcgg gtgagaggtg atcgaggatc gccgcctcga ccggccgctc gaggaactcg
    14521 tccgggtggc ggcagaagta ctggtccagg ccgtccgggc cggcgatgta gacagcgagc
    14581 ccgcgttcac ggcgtccggc ccggccccac atctgcttca ggccggccac ggtgccggga
    14641 aaggtgacgc agatcgcggc gtcgaggccg ccgatgtcga tgccgagctc cagcgcgtcg
    14701 gtcgaggcga ccccgagcag ctcgccggaa acgagcttcc gctcgatttc gcggcgctgg
    14761 gccggggtgt accccgcccg gtagggagcg atccgctcgg ccaggtcagg ccggcccttt
    14821 tcggtcagcc ggtcggcggc gaacttgcgg atcagctcga tccccttgcg ggacttcaga
    14881 aagcagatcg tccgtacctc gtgggagacc aggtcggcca tcaggtccgc cgcctcggcc
    14941 agggcggagc gtcgcgcgcc gacctgtgga ttgacgagtg gcgggttcca gatccggatc
    15001 tcccggccgg ctcgcggggc gccgtcctcg tcgatcaggc gagccgggct gccggtcagc
    15061 cgctcggcca gctccgcggg gttggcgatc gtcgccgagg ccatcacgaa gcggggctcc
    15121 ctgccgtagg cccgggccag ccgcctgagc cgtcgcagca cgttcgcgac atgcgagcca
    15181 aagactcccc ggtaggtgtg ggcctcgtcg acaaccaccc agtcgaggtt ggcgaggaag
    15241 tcaccccagc cgcggtggtg cggcatgatc ccgacgtgga gcatgtccgg gttggtcagg
    15301 accaggttgc tctggcggcg aatcgccggg cgctcgtccc gcggggtgtc accgtcatag
    15361 atcgccagtc gcaggttcgg tgaaccgagc cgggccaggg cgcgggcctg gtcctgggcg
    15421 agcgccttgg tcgggtagag gtagagcgcc cgcgccttgg ggtcggctgc gatcgcgttg
    15481 agcggaggca ggttgaaggc cagcgacttg ccggaagcgg tcgcggtggt caggatcacg
    15541 ttgccctcgg ccgccgcctc gaaggcctcg gcctgatggc tgtaaagctg ttcgatgccg
    15601 ccccggttca g
//
LOCUS       DAOQKN010000037        15457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_1755_length_15457_cov_6.178938, whole genome shotgun sequence.
ACCESSION   DAOQKN010000037 DAOQKN010000000
VERSION     DAOQKN010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 15457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15457
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1755_length_15457_cov_6.178938"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="PLJ64_04090"
     CDS             <1..437
                     /locus_tag="PLJ64_04090"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNC92702.1"
                     /translation="ANRRRSAAYRPLPTAQADRPKKRTAKPQDVFGGEMRRRDLLPLL
                     IFHLIRAEPSYGNQLIDAIETLTQGVISVNPNTMYPLLRDLEGKGMIEGSWEHPDKRT
                     RRFYSITESGEAAYQELLPEIEPFLDSVIRSVTMIKAEVYGS"
     gene            525..728
                     /locus_tag="PLJ64_04095"
     CDS             525..728
                     /locus_tag="PLJ64_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNC92703.1"
                     /translation="MPTGTVKWFSDEKGFGFITPDDGSKDVFVHHSAIQADGFRTLAE
                     GAKVSYESEEGPKGPAAASVETI"
     gene            827..2155
                     /locus_tag="PLJ64_04100"
     CDS             827..2155
                     /locus_tag="PLJ64_04100"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC92704.1"
                     /translation="MYSTGGVADRFIPLWSRIEGRLGGSLPAAIRFWDGSEIGPGEAG
                     SCPDVIVLRSKRAMTYVVTRPDQLGVARAFVSGDLVLEGSIERVMAAGARLYGFDFTM
                     QDKLEAVKLAVGLGAIKVPPPKPPESEARPKGRLHSLRRDSESIQHHYDVSNDFYRLI
                     LGPTMVYSCAYFNSADDSLEEAQTRKLDVVCQKLRLEPGERLLDIGCGWGSMAIHAAS
                     NYGVKVVGVTLSIAQAERARQRVAEAGLEDRIEIRIQDYREVSDGPFDKLCSIGMFEH
                     TGSDIDGYFAETAKLGKPGGLALHHGICRQHSDEESPNTFINHYVFPDGELHRVARVI
                     AGLERSGQELRDVENLREHYALTLRHWVANLAANQEAAIAEVGEERERIWRLYMTASF
                     LAFERGDVGVNQILGVIPEHEQVALYRPSPGLALARPSFVREEVSAGQVG"
     gene            complement(2135..4837)
                     /locus_tag="PLJ64_04105"
     CDS             complement(2135..4837)
                     /locus_tag="PLJ64_04105"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNC92705.1"
                     /translation="MTPERRQPKDKTQPTQEVLLNDLRRSRSVGEAAEVTARAAVEQF
                     GAVGAVVSDRQGPIAARPTRLDPLTIAELTAIPEGVGVGSRGSVLEGMSVACFSHPEM
                     DGIRLAAAWAPESSARVDDLEAGINSFAVWLRQFANPAAERVKRSYTGPGPTATDYEQ
                     SRKRMDAEGFEHLIDSVPAILYTAEMGESGQWLYVSPQIEKILGYTPEEWLSDTTIWY
                     RSIHPDDREHALAFEDERLIGLDLHPPAEYRLKARDGRYVWIYERARLVRNEQGVPQW
                     HGVMQDISALKAAETAIELKAEQQVLVARLGEMAIRGEDPDSLLRYAVDWVGQLDGVI
                     EASIWEQEDYKELHLHHRSANLGWSNTLPYDPEQYPDSHLTQGDVVLIPSWEDDPRLE
                     RFVPFLPESVRSSMIAPIMGAVDQFGMIIVHSDRISAFSEQDGNFLRAAANVLGNSIE
                     RSRTDLSLQHRINHDPLTDLPNRQLFTRRLAEAIDEAGSREGIVALLFLDIDHFKLIN
                     DGIGHHAGDELLREIAPRLSASVRRGDTVARFGGDEFGIVLASIKSVDEAQEVARRML
                     ESISEPIPVAGTERFVTASIGVATYQPGREEPKSAEDLIRDADAAMYLAKDSGRARVA
                     SFGKPIRDRVLRRLDVERQLHGAIENEELQVVYQPIISLRTGKLRAFEALTRWDHPEE
                     GILKPELFIPIAEESDLISRIDSWVMAEAVRQAGVWNEGLDPDDRITVSVNASSRQIH
                     KSGLAAEITDLLVRCDMRPENLAIEITENVLLTGSAVIDDVLREIHELGVRLALDDFG
                     TGYSSLSYLSEFPLDAIKIDRAFVEGLGRGDPSTSAIADAIAQIGRALSLLVVAEAVS
                     TETQLQMVRDLGCSMAQGFLISPPLSVEQATDFVKGSGLPDLTR"
     gene            complement(4834..5982)
                     /locus_tag="PLJ64_04110"
     CDS             complement(4834..5982)
                     /locus_tag="PLJ64_04110"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNC92706.1"
                     /translation="MSGEEYNFTDLSLDRLRRRRSAKWTFWPDDVLPAFVAEMDFPLA
                     RPVKEALVQAIELDDTGYSNASASGLADAFAAFADRRFGWEVDPEQVTATTDVVGGLR
                     ALLDLATGPLEQVIINPPVYFPFFSIIPEVGSEILEVPLDEKGRLDLDGTAAAFAGGA
                     RAMILCSPHNPTGSVPARTELERLAEIAAESDAWILADEIHAPLTLPGATHTPFLDVS
                     PAARECGICVTSASKAFNIAGLSCAVIVTASERARRLVSRLPAGATHPGHLGVIASQA
                     AFESGDQWLDQVRDQLDFNRRLLSDLLAGHLPAARYRPPEAGYLAWVDAGELDLGPDP
                     ASEILDRARVAVSSGPGFGSGGEGYFRLNIGTSPQLVEAAVRSISEIA"
     gene            complement(5979..7193)
                     /locus_tag="PLJ64_04115"
     CDS             complement(5979..7193)
                     /locus_tag="PLJ64_04115"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC92707.1"
                     /translation="MTATTTEADDMDRRSIAVLAAGHAMVDACQGAIPATLPFLIARR
                     GYSYAEATSLLLVMTVCSSLLQPLFGYITDRRSISWLLPGGVLLGGLGVAAVGLVHSH
                     ALTLAAVGIAGLGVGAYHPEGARNANFVAGNRRRATAMSLYGVGGNAGFAVGPVIVTG
                     LVLAFGLGGLVWFALPMTLGAIWLARELPRIDSLRLATIAGAEAQPGGRGEDRWLPFA
                     GIATAAGFRSAAYFGLQAFVPIFLITTMGTSDAVGNAALTVLLASGVAGTLLGGALAD
                     RVGNKPVLVTSLGVASPLILGLLAVNEAGAFALVALIGFFLFGSFSIAVVLGQQYLPN
                     RIGVASGVTLGAAIGFGGLVAWLLGLLADQTSLTTVMIVIAALPLPGFLISLALPGEN
                     EAGQPSEEPARV"
     gene            7239..7700
                     /locus_tag="PLJ64_04120"
     CDS             7239..7700
                     /locus_tag="PLJ64_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92708.1"
                     /translation="MTNYPDYGSPVRKAREMARAALPCESCGAGGEPVIRRPRPYSSG
                     ESAKAGAFFACPECGHRLSELDGAAADRLSDVPEDNLPDPAGMLARRIQPLVGEARPG
                     DRAKVLAQAVTGLAREHGLGCRTDGEFVLIWRGEPWLELGLEGDRVVILPG"
     gene            complement(7704..8732)
                     /locus_tag="PLJ64_04125"
     CDS             complement(7704..8732)
                     /locus_tag="PLJ64_04125"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNC92709.1"
                     /translation="MNTRFHVPSKVFGTRRSRGERPDGDPGSQSETSVVPASITGTDP
                     LLIDPMLNGSPGKSCSYLIQGEKNVLIDPGSANAAPRIIEALKSNRIKVDLILLTHTH
                     PDAAAGVGVVAREFPNAEIASPAGATEQLADPSAMVSGMRKVYGEKVEKLYGLPAPVD
                     SIRIHAVGDGEQVDLGDRAIEAIATPGHTAAHMSYLDPESSSLFCGDALGIQLPGSSV
                     VRPSTPPWDFSLEDSLDSIERLAGVEAETVYLAHYGAARPGPTEIFDRAADSLERWHK
                     SFLKKREQTDSEEDLSRQFNACLEASLEPIAPSVRRDLETVSPAWLNLAGLSAEQARL
                     NGRLSDAA"
     gene            complement(8880..11237)
                     /locus_tag="PLJ64_04130"
     CDS             complement(8880..11237)
                     /locus_tag="PLJ64_04130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HNC92710.1"
                     /translation="MKALVTFAATAAVLFSASALAKAAPGSVNPDFGKNGFVRVSTSP
                     GQKTIVRDMIRTASGNLLLSGGAGVAGNGFLLRLLPDGSRDPEFGNGDGLVLTPASQW
                     AKLELQGERIVAFGRSGSDPVVARFESDGTLDESFGDQGTVLLDVRSRFGEDADQVLE
                     TPGPGIDSEGRIVAGSTVASCATTRIEPDHGRHRCGNVVLFRFSPDGQPDTDYGNGGV
                     VMLGAGWNAGGRSAALALDGQDRVLVRTRDSVYYGSPQRTRNDVLQRFLPSGAYDHSF
                     GADGIVDLKVWAGEPGVANRGRILEGPDDRISVAANFFARVGRSGQVLSRPRTGSVDS
                     LANGTYTSMTDVEFDRQGRMIISGTSNTSGSKRQFVVSRFGTDGNLDGSWSRDGVATA
                     RLGDRLKSGLMSRRRGIIEAASVLNSDGGVTVAATAIRGDRWQIELTRFSGGNGKPAF
                     CRGRRANVLGTSGDDVLKGGYDSVIVAGAGDDKVRAGSKAVICLGSGDDELRAGDDAD
                     KRPLVFGGSGNDRIKSGGRIIGGPGRDEINATSSARVFAGPGDDDVRAFGGFEINGGA
                     GDDTLKARYGGRLNGGPGNDHLIGSDTVGDLTDILDGGPGDDRLEGNGRPDRLFGRAG
                     DDALDGGTGPDLLVGGTGRDQIDPGPPIADRQIYAVDNRKARGRVWINGNQWSAGDVQ
                     VWYRCSGWNYGTVRDSFHGSSTFNEDLKFNSRGRAKVDFDFWDGFGFDSDGWMRARKT
                     GRRIFVTYWGREDFSYGEDGSFKCRTGLLKFSLRRVAGERDIVRP"
     gene            complement(11414..11617)
                     /locus_tag="PLJ64_04135"
     CDS             complement(11414..11617)
                     /locus_tag="PLJ64_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNC92711.1"
                     /translation="MPTGTVKWFSDEKGFGFITPDDGSKDVFVHHTAIQSDGFRTLAE
                     GAKVSYEAEDGPKGPAAASVEAI"
     gene            complement(11771..13147)
                     /locus_tag="PLJ64_04140"
     CDS             complement(11771..13147)
                     /locus_tag="PLJ64_04140"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNC92712.1"
                     /translation="MSQQSFADLGVSNAVVNSLRDRGIEAPFPIQRQVIEDILEGDDV
                     LVQSPTGSGKTLAFGVPLVDLVESTDPRPTALILAPTRELASQIVDEMEAICHARALR
                     IAAVYGGVGLQQQAKRAAKSHIIVACPGRLEDQLQRGAFDLENVRYLVLDEADRMLDM
                     GFKPAVDRIVRQIDDSRQTLFFSATLEGAAGKLAAAYTIDPKTHVNRTPKEKQGTIEH
                     RFIHVSHEAKVETLVGELESPDRGRTLVFVRTKHGADRLVKKLSKRSRQVRAVAMHGN
                     KSQNQRQRALADFEDGRVDTLVATDVAARGIDVADVTHVINFDIPEDQDTFVHRVGRT
                     GRAGADGVGISFFMSDQAKEMGSIAKSLGLHQEFEASGAGFHAESHTRQKRSGGNGSR
                     SGTRKPGSGPSRKGGRPQQSRAGGGKGSPQGRAEGDDRQGSRGGKPKRNRSNASNRNR
                     NSRRQRSR"
     gene            13450..>15457
                     /locus_tag="PLJ64_04145"
     CDS             13450..>15457
                     /locus_tag="PLJ64_04145"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNC92713.1"
                     /translation="MFVHKAAVVGGGTMGGQIAQAIAAADIPVVVKDVDQKFVDHAIE
                     EVRNVTAGQLASKVKKEKITQEQADARLEEVLANVTGTVTYDEFGDVDFVIEAVPEKM
                     AIKQAVFAELDAVTPGHAILSSNTSALSISEIGEATLRPDKVVGFHFFYPASVMPLVE
                     VVIGDDTSSETAQTSSNFAQAIGKSPITCGEVPGFVVNRILNSAVGEIWRAQEERGLS
                     IAKIDEGVGAANVSPMGPFILIDVLGLDTVYHVAEHLNDSYGERFYVHQGMKQLVADG
                     KLGAKTGGEGMYKDGQPNVPGDADADAQELTDLFLAKSLVEACLILEEGACSVRDIDW
                     GMMNGAGLNPMKGLFPPFWKADLEGLDVSLERIEKLNEKYGDERFPVPVTLKRLVAQG
                     RLGLSTGQGFYPYPQPDEGDQPEKVKLETRGNVAIAWLANPPMNAISPQVIEDLGTVW
                     EKVNANPEIGAMVIASSTGPIVYSAGADIKAFTKMDTEGGAALINNGHELLKSFGSGR
                     VATIAAVNSLAFGGGCELAMACDFRIAAESAVFGQPEIKLGIIPGFGGTQRLPRLVGW
                     GKALEMNTVGDPISSIEAFEAGLVNELVPDQELFDVALNWASRLAGQAPLAVEQVKQV
                     SFRGGDPDAGIDAEKQGFAKVFFSEDGKEGISAFLGKRKPDWQGK"
BASE COUNT         2632 a         5080 c         5111 g         2634 t
ORIGIN      
        1 ctgcgaaccg ccgacgatct gctgcctacc gcccactgcc cactgcccaa gccgaccgcc
       61 cgaagaagcg gacggcaaag ccgcaggacg tgtttggtgg cgagatgcgc cggcgggatc
      121 tcctgccgct gctgatcttc cacctgatcc gggccgagcc ttcctacggc aaccagctga
      181 tcgacgcgat cgagaccctg acccagggcg tgatctcggt caacccgaac acgatgtacc
      241 cgctgcttcg cgacctcgaa ggcaagggga tgatcgaagg cagctgggag caccccgaca
      301 agcggacccg ccgcttctac tcgatcaccg aatctggcga agccgcttac caggaactgc
      361 tgcccgagat cgagcctttc ctggattcgg tgatccggtc ggtgaccatg atcaaggccg
      421 aggtttacgg ctcctgaagc gccgcccggc gctcaagtgg taatttgccc cgcgtcaggc
      481 cgggaggaga gaaccggcac cgcatcatga tgttggagga agttatgccg actgggacag
      541 tcaagtggtt cagtgacgag aagggcttcg gattcatcac acctgatgat ggctccaagg
      601 acgtcttcgt ccaccactcc gcgatccagg ccgacgggtt tcgcaccctg gccgagggcg
      661 ccaaggtgag ctacgagtcc gaagagggcc cgaagggccc cgcagccgct tccgtcgaga
      721 ccatctgaac ccggtctcgg aagctgttcg aaacggccgc ccccgggcgg ccgttttcat
      781 tgcagctcct tgagccgagg tcgggccggg agtaactttc gtgggcatgt acagcacagg
      841 tggtgtcgcc gatcgtttca ttcctctctg gtcccggatc gaaggcaggc tgggcgggtc
      901 gctcccggcc gcgatccgct tctgggacgg cagcgagatc ggaccgggcg aagcgggttc
      961 ctgccccgac gtgatcgtgc tgcgaagcaa gcgggcgatg acctatgtgg tgacccggcc
     1021 ggaccagctc ggggtggccc gggccttcgt ctccggtgac ctcgtgctcg aaggcagcat
     1081 cgagcgggtg atggcggccg gagcccggct ttacggattt gacttcacga tgcaggacaa
     1141 gctcgaggca gtgaagctgg cggtcggtct gggagcgatc aaggtgccgc cgccgaagcc
     1201 gccggaatcg gaggccaggc cgaagggaag gctgcattcg ctccgtcgcg actccgagtc
     1261 gatccagcat cactacgacg tctcgaatga tttctaccgg ctgatcctcg gcccgaccat
     1321 ggtctactcc tgtgcctact tcaactcggc cgacgacagc ctcgaagagg cccagacccg
     1381 caaactggac gtggtctgcc agaagctcag gctcgaaccg ggcgagcggc tgctcgacat
     1441 cggctgcggc tggggatcga tggcgattca tgcggcatcc aactacggag tgaaagtggt
     1501 cggggtcacc ctttcgatcg cccaggcgga gcgggccagg cagcgggttg ccgaagcggg
     1561 gctcgaggac aggatcgaga tccgcatcca ggactaccgc gaggtcagcg atggtccctt
     1621 cgacaagctt tgctcgatcg gcatgttcga gcacaccggt tccgacatcg atggctattt
     1681 cgcggagacc gcgaagctgg gcaagccggg cggcctcgca cttcaccacg ggatctgcag
     1741 gcagcacagc gatgaggaat caccgaacac gttcatcaac cactatgtat ttcccgacgg
     1801 cgagcttcac cgggtcgccc gggtgatcgc cgggctcgaa cgctccggcc aggaactgcg
     1861 ggacgtcgag aacctgcgcg agcattacgc gctcaccctg cgccactggg ttgccaatct
     1921 cgccgccaac caggaagcgg cgatcgccga ggtcggggaa gaacgggaac ggatctggcg
     1981 gctctacatg accgccagct tcctcgcctt cgaacgcgga gacgtcggcg tcaaccagat
     2041 tctcggggtg attccggagc atgagcaggt tgccctgtac cggccctcgc cgggactggc
     2101 gctggcccgg ccctccttcg tgcgggaaga ggtctcagcg ggtcaggtcg ggtaggccgg
     2161 agcctttgac gaagtcggtc gcctgctcga cactgagcgg cggcgagatc agaaagccct
     2221 gcgccatcga gcagccgagg tcccggacca tctgaagctg ggtttcggtc gagactgcct
     2281 ccgccacgac cagcagcgaa agcgcccggc cgatctgggc gatcgcgtcg gcgatcgcag
     2341 aggtgctggg gtcaccgcgg ccgagaccct cgacgaaggc ccggtcgatc ttgatcgcgt
     2401 caagcggaaa ctcgctcagg tagctgagtg acgagtagcc ggtgccaaaa tcgtcgagcg
     2461 cgagccggac gccaagctcg tgaatttccc gcagcacgtc gtcgatcacg gcacttccgg
     2521 tcagcaggac gttctcggtg atctcgatcg ccaggttttc gggccgcatg tcacatctga
     2581 ccagcagatc ggtgatctcg gccgcgagac cggatttgtg gatctggcgc gatgaagcgt
     2641 tgaccgaaac ggtgatccgg tcgtcgggat cgaggccctc gttccagacc cccgcctgcc
     2701 tgaccgcctc ggccatcacc cagctgtcga tccgggaaat caggtcgctc tcctcggcga
     2761 tcgggatgaa gagctccggc ttgaggatgc cctcctcggg atgatcccag cgggtcagcg
     2821 cctcgaaggc ccgcagcttg ccggtgcgca agctgatgat cggctggtag acgacctgca
     2881 gctcctcgtt ttcgatcgcg ccgtgaagct ggcgctcgac gtccagtcga cgcagcaccc
     2941 ggtcgcgaat cggtttgccg aaggacgcga cccgggcccg gccggaatcc ttggccaggt
     3001 acatcgccgc atcggcatcg cggatcaggt cctcggccga tttcggctct tcccggcccg
     3061 gctgataggt ggcgaccccg atactggcgg tgacgaatcg ctcggtcccg gcgaccggga
     3121 tcggctccga aatgctctcc agcatgcgac gggccacttc ctgcgcctcg tcgaccgact
     3181 tgatcgaggc gaggacgatc ccgaactcgt cgccaccgaa ccgggccacc gtgtccccgc
     3241 ggcggacgct ggccgaaagc cgcggcgcga tctcgcgcag cagttcgtca ccggcgtgat
     3301 gcccgatccc gtcgttgatc agcttgaagt ggtcgatgtc gaggaacagc agggcgacga
     3361 tgccttcccg cgagcccgcc tcgtcgatcg cctcggccag ccggcgggtg aacagttgcc
     3421 ggttgggcag gtcggtcagt ggatcgtggt tgatccggtg ctgaagcgaa aggtcggtcc
     3481 ggctgcgttc gatcgagttg ccgagcacgt tggcggcggc ccggaggaag ttgccgtcct
     3541 gttcgctgaa ggcgctgatc cggtcggagt ggacgatgat catgccgaac tggtcgaccg
     3601 cgcccatgat cggggcgatc atcgaactgc gaaccgattc gggcaggaag ggaacgaaac
     3661 gctcaagccg cggatcgtcc tcccaggagg ggatcaggac cacgtcgccc tgggtgagat
     3721 ggctgtccgg atactgctcc gggtcatatg gcagcgtgtt cgaccagccg agattggcgg
     3781 agcggtggtg gaggtgcagc tccttgtaat cctcctgttc ccagatcgat gcctcgatca
     3841 cgccgtcaag ctgaccgacc cagtcgaccg cgtagcggag cagggaatcc gggtcttcgc
     3901 cgcggatcgc catctcgccc agccgcgcga ccagaacctg ctgttcggcc ttgagttcga
     3961 tcgcggtctc ggccgccttg agtgccgaga tgtcctgcat caccccatgc cactgcggca
     4021 ctccctgctc gttgcggacc agacgagcgc gctcgtagat ccacacgtaa cggccgtcgc
     4081 gggccttcag gcggtattcg gccgggggat ggagatcgag tccgatcagc ctttcgtcct
     4141 cgaacgcgag cgcatgctcg cggtcgtccg ggtggatcga ccggtaccag atggtggtgt
     4201 ccgaaagcca ttcttccggg gtgtagccca ggatcttctc gatctgcggg cttacataaa
     4261 gccactgccc ggattcgccc atctcggcgg tgtagaggat ggcgggcacc gagtcgatca
     4321 ggtgctcgaa cccttccgcg tccattcgct tgcggctctg ctcgtaatcg gtagcggtcg
     4381 ggccggggcc ggtgtaggag cgcttgaccc gttcggcggc cggattggcg aactgcctga
     4441 gccagaccgc gaaggagttg attcccgcct cgagatcgtc gacccgggct gaactctcgg
     4501 gcgcccatgc ggcggccagt cgtatgccgt ccatttcggg atgggagaag caggcaacgg
     4561 acatgccttc gaggacgctt ccgcgggaac cgacgccgac tccttcggga atcgccgtaa
     4621 gttcggcgat ggtcagcggg tcgagcctgg tcggccgggc ggcgatcggg ccctggcggt
     4681 cactgaccac cgcgccgacc gctccgaact gttcgaccgc cgcccgggcc gtcacctcgg
     4741 cggcctcgcc gaccgaccgt gaccggcgca ggtcattgag gagtacctcc tgcgtgggct
     4801 gcgtcttgtc tttgggctgg cgccgttccg gagtcatgcg atctctgaga ttgaccttac
     4861 ggcagcctcg accagttgtg gtgaagtgcc aatgttgagg cgaaaatacc cttcgccacc
     4921 actgccgaat ccgggtccgc tcgaaaccgc gacccgggcc cggtcgagga tctccgaggc
     4981 gggatcgggg ccgagatcga gctcgccggc atcgacccag gccaggtatc cggcctcggg
     5041 cggccggtat cgggcggcgg gtagatgccc ggcgagcagg tcggaaagca gccgccggtt
     5101 gaagtcgagc tggtccctga cctggtcaag ccactgatct ccggattcga atgccgcctg
     5161 cgaggcgatc accccgaggt gacccggatg ggtggcgccg gccggcagcc gggaaacgag
     5221 ccgcctcgcc cgctcgctgg ccgtcacgat caccgcgcag ctcaaaccgg cgatgttgaa
     5281 cgccttcgag gccgaggtaa cgcagatccc gcactcgcgc gctgccgggg agacatcgag
     5341 gaacggtgtg tgggtcgctc ccggcagggt gagcggcgcg tggatctcgt cggccaggat
     5401 ccaggcgtcg ctttcggcgg cgatttccgc cagtcgttcc agctcggtcc gggccggaac
     5461 ggagccggtc gggttgtgcg gactgcagag aatcatggcc ctggcgccgc cggcgaaggc
     5521 ggcggcggtc ccgtcgagat cgagccggcc cttttcgtca agcggcactt ccaggatttc
     5581 gctgccgacc tcgggaatga tcgagaagaa cgggaaatag accggcggat tgatgatcac
     5641 ctgctccagc ggaccggttg cgaggtcgag cagcgcccgc agcccgccga ccacgtcggt
     5701 ggtggcggtg acctgttccg gatccacctc ccagccgaag cgacggtccg cgaaagccgc
     5761 gaacgcgtcg gcgagcccgg aggcggaggc gttggaatag ccggtgtcat cgagttcgat
     5821 cgcctggacc agggcctcct tgaccggccg ggccaggggg aaatccatct ccgcaacgaa
     5881 ggctggcaag acgtcgtcag gccagaaggt ccacttggcc gagcgacggc gccgcagccg
     5941 gtcgagagaa aggtctgtga agttgtactc ctcgccgctc aaacccgggc tggctcctcg
     6001 cttggttggc cggcctcatt ttctccgggc agcgccaggc tgatcaggaa tcccggtagc
     6061 ggcaaggcgg caatcacgat catcaccgtg gtcaggctgg tctgatcggc cagcagtccg
     6121 agcagccagg cgaccagccc gccgaagccg atggcggcgc cgagcgtgac cccggaggcc
     6181 acaccgatcc ggttgggcag gtactgctgg ccgagaacga ccgcgatcga gaagctgccg
     6241 aagaggaaga acccgatcag ggccaccaag gcaaaagcac cggcttcgtt tacggccagc
     6301 agcccgagga tcagggggct ggccacgccc agcgaggtca ccaggaccgg cttgttgccg
     6361 acccggtcgg ccagtgcccc gcccagcagg gttcccgcaa ccccgctcgc cagcagcacc
     6421 gtcagggcgg cgtttccgac cgcgtccgag gtgcccatgg tcgtgatcag aaagatcggc
     6481 acgaaggcct ggagtccgaa atacgcggcg gagcggaacc cggccgccgt cgcgatcccc
     6541 gcaaacggca gccagcggtc ttcaccgcga cctccgggtt gcgcttcggc tcccgcgatc
     6601 gttgccaggc gcagggaatc gatgcggggc agctcgcggg ccagccagat cgcacccagg
     6661 gtcatcggca aggcgaacca gaccagtccc ccgagaccga aggcaagaac gagcccggtc
     6721 acgatcaccg ggccgaccgc gaaacccgcg ttgcccccga ccccgtaaag gctcatcgcc
     6781 gtcgcccttc gcctgtttcc ggccacaaag ttcgcgttgc gggccccttc ggggtggtag
     6841 gcgccgactc ccagaccggc gattcccacc gcggcaaggg tcagggcatg cgagtggacc
     6901 agcccgaccg cggccacacc gagcccgccg agcagcaccc cgccgggcag aagccatgag
     6961 atcgaccggc ggtccgtgat gtagccgaag aggggctgaa gcagggacga gcagacggtc
     7021 atgaccagca gaagcgaagt cgcctccgcg tagctgtaac cgcgacgggc gatcaggaag
     7081 ggcagcgttg cagggatcgc tccctggcag gcatcgacca tggcgtggcc ggccgcgagc
     7141 acggcgatcg agcgccggtc catgtcatcg gcctctgtgg tcgttgcggt caccggtcaa
     7201 gcctgtcaga agttccgccc gggaccgacc cgaaccccgt gaccaactat ccggattacg
     7261 gctcgccggt gcgaaaggcc agggaaatgg cccgggcggc gctgccttgc gaaagctgcg
     7321 gggccggggg cgagccggtg atccggcggc cccgccccta ctcgtccggg gaatccgcga
     7381 aggcgggcgc cttcttcgct tgccccgaat gcggtcatcg cctgtcggag ctggacggcg
     7441 cggctgccga ccggctttcg gatgtccccg aagacaacct cccggacccg gccgggatgc
     7501 tcgccaggag gattcagccc ctcgtcgggg aggcccggcc cggcgaccgg gcgaaggttc
     7561 tggcccaggc cgtgaccggc ctggcccggg aacacgggct cggttgccgc accgacgggg
     7621 aattcgtcct catctggcgg ggcgagccgt ggctggagct cggcctcgaa ggtgaccggg
     7681 tggtaatcct gcccggttag ggttcaggcc gcgtcggaaa ggcggccgtt gaggcgggcc
     7741 tgttcggcgg agagcccggc caggttcagc caggccgggc tgaccgtctc gaggtcgcgg
     7801 cgaaccgaag gggcgatcgg ctccaggctc gcttcgaggc aggcattgaa ctgccgggag
     7861 aggtcctctt cgctgtcggt ttgttcccgc ttcttcagga acgacttgtg ccagcgctcg
     7921 agtgaatcgg cggcccggtc aaagatctcg gtcggcccgg gacgagccgc cccgtaatgg
     7981 gcgagataga ccgtttcggc ttcgaccccg gccagcctct cgatcgaatc cagcgaatcc
     8041 tcaagcgaga aatcccaggg cggggtggac ggccggacga cgctgctgcc gggcagctgg
     8101 attccgagcg cgtccccgca gaaaagcgat gaactctccg ggtcgaggta agacatgtgc
     8161 gcggcagtgt ggcccggagt cgcgatcgcc tcgatcgccc ggtcgcccag atcaacctgc
     8221 tcgccgtcac caaccgcgtg gatccgaatc gaatcgaccg gtgccggaag cccgtaaagc
     8281 ttctcgacct tttcgccgta gaccttgcgc atcccggaaa ccatggccga gggatcggca
     8341 agctgctcgg tcgctccggc cggcgaggcg atttccgcgt tcgggaattc gcgggcgacg
     8401 acaccgaccc cggcggccgc gtccgggtgg gtgtgcgtga ggaggatcag gtcgaccttg
     8461 atccggttcg atttcagtgc ctcgatgatc cggggcgcgg cattcgccga tcccgggtcg
     8521 atcaggacgt tcttctcgcc ctggatcagg taggagcagg acttgcccgg gctcccgttc
     8581 agcattggat cgatcagcag cggatcggtg ccggtgatcg aggcggggac caccgaggtt
     8641 tcggactggc ttccggggtc accgtcaggt cgctcgccgc ggctgcggcg tgtgccaaag
     8701 accttgctcg ggacgtggaa gcgcgtgttc atctcatcct ctgagttctc taaccaacca
     8761 tcttccagat ggatgcggtt ttccctctaa atacccactt aacgtttgtc gagaaccaca
     8821 atgccggacc cctgcggacg ccccgttgac cccggcgtca ggttcgttcg atccgaactt
     8881 cagggacgca cgatgtcccg ctcgcccgcc acacgccgca gcgagaactt cagcagcccg
     8941 gttcggcact tgaaggagcc atcctcgcca taggaaaagt cttcccggcc ccaataggtg
     9001 acgaagattc tccgcccggt cttccgtgcc ctcatccagc catcggaatc gaagccaaat
     9061 ccatcccaga agtcaaaatc gaccttggcc cggccccggc tgttgaactt caggtcttcg
     9121 ttgaaagtgg aggatccgtg gaagctgtcc cggacggttc cgtagttcca gccactgcac
     9181 cggtaccaga cctggacatc tcccgccgac cactggttgc cgttgatcca tacgcgtcct
     9241 ctcgccttcc ggttgtcgac cgcgtagatc tgccggtccg cgatcggcgg gcccgggtcg
     9301 atctggtcgc gaccggtgcc gccgaccagc aggtcggggc cggtaccgcc gtcgagggcg
     9361 tcgtcgcctg cccttccgaa gagacgatcg gggcggccgt tgccttcgag gcgatcatcg
     9421 cccggaccgc cgtcgagaat gtcggtcagg tcaccgaccg tgtcactgcc aatcaggtgg
     9481 tcgtttcccg ggccgccgtt gagccgtccc ccataacggg ctttcagggt gtcgtcaccc
     9541 gcaccgccgt tgatctcgaa accgccgaaa gccctgacgt catcatcgcc tggtccggcg
     9601 aataccctcg ccgaagatgt ggcgttaatt tcgtccctgc cgggaccgcc gatgatccgc
     9661 ccgccggact tgatccggtc gtttccggat cctccgaaga caagcggtcg cttgtccgca
     9721 tcatcgccgg ctcgcagctc gtcgtcaccg gagccgaggc agatcactgc tttcgagccc
     9781 gcacgcacct tgtcgtcacc cgcaccggcc acgatcacgg agtcgtagcc tcccttgagc
     9841 acgtcgtcac cggaggttcc cagaacattc gcccggcggc cgcggcagaa ggccggcttg
     9901 ccgttcccgc ccgagaacct ggtcagttcg atctgccagc gatctccccg gatcgccgta
     9961 gccgccacgg tcacgccgcc gtcggagttc agaaccgagg cagcctcgat gattccgcgg
    10021 cgccgggaca tgaggccgga cttcaaacgg tccccgagcc tggccgtcgc gacaccgtcc
    10081 cggctccagc tgccgtcgag gttgccatcg gttccgaacc tggagacgac gaactgtcgc
    10141 ttgctgccgg aagtgttgga ggttcccgag atgatcatcc gcccctgccg gtcgaactcg
    10201 acgtcggtca tgctggtgta agtgccattc gcgagcgagt cgacggaacc ggttcggggc
    10261 cgggacagga cttgaccgga ccgccccacc cttgcgaaga aattggcagc gaccgagatg
    10321 cggtcgtcgg gtccttcgag gattctgccg cggttggcga ctcccggctc gccggcccag
    10381 actttgagat cgacgatgcc atccgcgccg aagctgtggt cgtatgcgcc ggatggcagg
    10441 aaccgctgga gcacgtcgtt gcgggttctt tgggggcttc cgtagtacac gctgtcgcgg
    10501 gttcggacca gaacgcgatc ctggccgtcg agcgcgagcg cagccgacct gcctccggca
    10561 ttccagcctg ccccgagcat cacgaccccg ccgttgccgt agtcagtgtc gggttgaccg
    10621 tccggagaaa accggaaaag gacgacgttg ccgcagcggt gtcggccgtg gtccggctcg
    10681 atccgggtgg tcgcgcagct tgcgacggtg gagccggcca cgatcctgcc ttcgctgtcg
    10741 attcccggtc ccggggtttc gagcacctga tcggcgtcct ctccgaaccg ggacctcacg
    10801 tccagcagca cggttccctg gtcgccgaag ctctcgtcga gggtgccgtc cgattcgaat
    10861 ctcgcaacga cgggatctga accggaacgc ccgaaggcga cgatcctctc gccctggagt
    10921 tcgagcttcg cccactgcga ggcgggagtc aggacgagtc cgtcgccatt cccgaattcc
    10981 ggatcccggc ttccgtccgg cagcaaccgg agcaggaacc cgttccccgc cacgccagcg
    11041 cctccggaaa gaaggagatt gccggaagcg gtgcggatca tgtccctgac gatggtcttc
    11101 tggcccggac tcgtggaaac cctcacgaat ccgttcttgc cgaagtcggg gttgaccgag
    11161 cctggcgcgg ccttggccaa cgccgaagca gaaaacagga cagccgcggt ggcggcgaac
    11221 gtgaccagtg ccttcatttt ttctcccggg gggtagtgcg gattccggtc cgcgatgacc
    11281 agataacccg ttgtcggcca ccgggttgcg gtcagcccgc tcaggtgcgg tcgcttaaag
    11341 cgaaacgccc gccgggtggc gggcgcgctc gcgttgcttc tccggggcgc gaagcgcccc
    11401 gtggtcagtc gacctagatg gcctcgaccg aagcggcggc cggacccttg ggtccgtcct
    11461 ctgcctcgta gctcaccttg gcgccttccg cgagggtgcg gaagccgtcc gactggatgg
    11521 cggtgtgatg gacgaaaacg tccttcgaac cgtcgtccgg cgtgatgaag ccgaagccct
    11581 tttcgtcact gaaccacttg actgtgccag taggcattta gtcctccaac atcggtgctg
    11641 cgaaacgcgg aggatgccgg taaagcaacg actgcgagcg ctacacacgt ctttgtcggg
    11701 gtttactccc cccgacccta tgagcggacc gtagcagtat ctgcccgatc tcgcttgttt
    11761 tgttacaagg tcagcggctg cgctgccggc gggagttccg gttgcggttc gaagcgttgc
    11821 tgcggtttcg cttcggcttg ccgccccggc tgccctggcg atcgtcgcct tcggcccggc
    11881 cttgcgggga gcccttgccg cctccggccc ggctctgctg gggccgcccg cccttgcggg
    11941 aggggccgct gcccggcttt cgggttccac tgcgggagcc gttgccgccg gagcgcttct
    12001 ggcgggtgtg gctttcggcg tggaacccgg cgccgctggc ttcgaattcc tgatggagcc
    12061 cgagcgactt cgcgatgctt cccatctcct tcgcctggtc ggacatgaag aagctgatcc
    12121 cgaccccgtc ggcgccggcc cggccggtgc ggccgacccg gtggacaaag gtgtcctgat
    12181 cctcggggat gtcgaagttg attacgtggg tcacgtcggc gacgtcgatg ccacgggcgg
    12241 cgacgtcggt ggcgaccagg gtgtcaaccc ggccatcctc gaagtcggcc aaggcgcgct
    12301 gccgctggtt ctgcgacttg ttgccgtgca tggcgaccgc gcgaacctgc cggctgcgct
    12361 tgctcagctt cttgaccagc cggtcggcac catgcttggt ccggacgaag accagggtac
    12421 ggccacggtc cgggctttcg agttcgccca ccagggtttc taccttggct tcatgcgaaa
    12481 cgtggatgaa acggtgctca atcgttccct gcttctcctt cggcgtccgg ttgacatggg
    12541 tcttggggtc gatcgtgtag gccgcggcca gcttgccggc agcaccttcg agcgtggccg
    12601 agaagaagag ggtctggcgg ctgtcgtcga tctggcggac gatccggtcg accgccggct
    12661 tgaagcccat gtcgagcatg cggtcggctt cgtcgaggac caggtagcgc acgttttcaa
    12721 ggtcgaatgc gccgcgttgg agctggtctt ccagccggcc cgggcaggcg acgatgatgt
    12781 gggacttcgc ggcccgcttg gcctgctgct gcaggccgac cccgccgtag acagcggcga
    12841 tccggagtgc gcgggcgtgg cagatcgctt ccatttcatc aacgatctgg ctggccagct
    12901 cgcgggtcgg ggcgaggatc agcgcggtcg ggcgagggtc ggtggattcg accaggtcga
    12961 ccagcggcac accgaaggca agcgtcttgc cggaaccggt gggggactgg accagcacgt
    13021 catcgccttc gaggatgtcc tcgatgacct gacgctggat ggggaacggg gcttcgattc
    13081 cgcgatcgcg gagcgaattg accacggcat ttgacacgcc gagatcggca aaggactgct
    13141 gggacatgct tttcaacttc ctgtgagggg taagggggag atccggtaac aaccccgacg
    13201 ccggtaagcc gcgcgaaaca ggaaggaaac agaacctcga tcgcgtccgt cagggactgc
    13261 gaccgcggca aacgataaca ggccggataa aggaaacctc tgcaaaggac ggatttacgc
    13321 ggtgctggcg gcgtgtcgac cacagtcccg atagccttat tgactcgcga gtcaacttct
    13381 cgcgcgcagt cgggtcagac acccgcaagg ctctcaactc accaagcccc cggaggacca
    13441 agaaacctca tgttcgtaca caaggcagcc gtcgtcggcg gtggcaccat gggcggtcag
    13501 atcgcccagg cgatcgccgc agctgacatc ccggtcgtag tcaaggatgt cgaccagaag
    13561 ttcgtcgatc acgcgatcga ggaagtcagg aacgtcaccg ctggccagct tgccagcaag
    13621 gtcaagaagg aaaagatcac ccaggagcag gccgatgccc gcctcgaaga ggtcctggcc
    13681 aacgtcaccg gcaccgtcac ctacgacgag ttcggtgacg tcgacttcgt gatcgaggcc
    13741 gtgccggaga agatggcgat caagcaggcc gtctttgccg agcttgacgc ggtcaccccc
    13801 ggccacgcga tcctttcttc caacacctcg gccctctcga tctccgagat cggcgaggcc
    13861 acgctgcgtc ccgacaaggt cgtcggcttc cacttcttct acccggcctc ggtcatgccg
    13921 ctggtcgagg tcgtgatcgg agacgacacc tcgtccgaga ccgctcagac ttcgtcgaat
    13981 ttcgcccagg caatcggcaa gtccccgatc acctgcggcg aggtccccgg attcgtcgtc
    14041 aaccgcatcc tcaactccgc ggtcggcgag atctggcgcg cccaggaaga gcgcggcctc
    14101 tcgatcgcca agatcgacga aggcgtcggc gccgcgaacg tttccccgat gggcccgttc
    14161 atcctgatcg acgtcctcgg tctggacacc gtctaccacg tcgccgagca cctgaacgac
    14221 tcctacggcg agcgtttcta cgtccaccag ggcatgaagc agctggtcgc cgacggcaag
    14281 ctcggagcca agaccggcgg cgaaggcatg tacaaggacg gccagccgaa cgttcccggc
    14341 gacgccgacg ccgacgcaca ggagctgacc gacctcttcc tcgccaagtc tctggtcgag
    14401 gcctgcctga tcctcgagga gggtgcctgc agcgtccgcg acatcgactg gggcatgatg
    14461 aacggcgccg ggctgaaccc gatgaagggt ctcttcccgc cgttctggaa ggccgacctg
    14521 gaaggtctcg acgtttcgct cgagcggatc gagaagctga acgagaagta cggcgacgag
    14581 cgcttcccgg tgccggtcac cctcaagcgc ctggtcgccc agggccgcct cggcctcagc
    14641 accggtcagg gcttctaccc ctacccgcag cccgacgagg gcgatcagcc cgagaaggtc
    14701 aagctggaga cccggggcaa cgtcgcgatc gcctggctgg ccaacccgcc gatgaacgcg
    14761 atctcgcccc aggtcatcga agacctcggc accgtctggg agaaggtcaa cgccaatccc
    14821 gaaatcggcg ccatggtgat cgcgtcctcg accggtccga tcgtctactc ggccggtgcc
    14881 gacatcaagg cgttcaccaa gatggacacc gagggtggcg ccgcgctgat caacaacggc
    14941 cacgagctcc tcaagagctt cggttcgggc cgcgttgcga ccatcgccgc cgtcaactcg
    15001 ctcgccttcg gcggcggttg cgagctcgcg atggcctgtg acttccgcat cgccgcggaa
    15061 tccgcggtct tcggccagcc ggagatcaag ctgggcatca tccccggctt cggcggaacc
    15121 cagcgtctgc cccgtctggt tggctggggc aaggccctcg agatgaacac cgtcggtgac
    15181 ccgatctcct cgatcgaggc gttcgaggcc ggtctggtca acgagctcgt gcccgatcag
    15241 gagctcttcg acgtcgccct gaactgggcg agccgtctgg ccggtcaggc cccgctcgcg
    15301 gtcgagcagg tcaagcaggt cagcttccgt ggcggcgacc cggacgccgg catcgacgcc
    15361 gagaagcagg gcttcgccaa ggtcttcttc tccgaggacg gcaaggaagg catctccgcc
    15421 ttcctcggca agcgcaagcc cgactggcag ggcaagt
//
LOCUS       DAOQKN010000038        15429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_1762_length_15429_cov_6.031156, whole genome shotgun sequence.
ACCESSION   DAOQKN010000038 DAOQKN010000000
VERSION     DAOQKN010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 15429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15429
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1762_length_15429_cov_6.031156"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..1562
                     /locus_tag="PLJ64_04150"
     CDS             174..1562
                     /locus_tag="PLJ64_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="fenitrothion hydrolase"
                     /protein_id="HNC92714.1"
                     /translation="MISLPLAHALVSRGDLPIPVWLFAWGAGVVLVVSFFALASAWRK
                     PRFEDAPGRPAATWLSATANSSAVQFLSGLIGVLLLAVVLYAGIEGTEAADRNFSLTF
                     VFVTVWLGFPVLSVVFGNVFAAFSPWRAIGRAYGWLAQRLAPGAVRHVEYPEKVGRWP
                     AAVGLVAFVWLEIIYGATGAAVVLSPEIVAKATVIYSVYTLVMMGVFGVEKWSSRGEF
                     FSVYFGMFASMSSFRKREGKLERRRWLSGTTDWANVPGSLALVVSAIGVTTFDGAQEG
                     ALKTPIEWTQERIGDLGFAATVSVRAGDTIFLLITIGIVALIYLLGVKGMTRVDAAPP
                     FKVLRSRFAHTLIPIALAYLVAHYFSLFVFQEQAQFTFLLSDPLGDGSNLFGTATSGI
                     DYTLIGPELVWYVQVGALIAGHVTALVLAHDRALLIWPDYRLATRSQYWMLAVMLCFT
                     SLGLFLLSFANQ"
     gene            1734..2855
                     /locus_tag="PLJ64_04155"
     CDS             1734..2855
                     /locus_tag="PLJ64_04155"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNC92715.1"
                     /translation="MSGSSSAADTQSKLDKAEKKIEEGQQREGVLTEKLDQLGERVDS
                     MQAAVDALREAEQEAAAKLQQAEADLKLAIERLQTSLEELRETRRRLKVSLQVLRQRL
                     VDIYVSGTPSIASMVLAANEYSDLVEMGPYLEAIQSRDESLVQRVRELKNEARKIVEA
                     RRDSKETIQTSRDSIAAETARLASARSETESQVASLASAKAASEEALNEVQDGIAQEE
                     EVAADLREKLTKELGTATSVPFSVGPASTPSSNSMIWPIDGTLTSPFGPRWGRTHEGL
                     DIAAPAGTPIAAAASGTVVLMQSEAESGGYGLFTCVDHGGGLSTCYAHQSEFGTSVGA
                     SVKQGEVIGYVGNTGNSFGDHVHFEVRINGAATDPMAYL"
     gene            complement(2852..3799)
                     /locus_tag="PLJ64_04160"
     CDS             complement(2852..3799)
                     /locus_tag="PLJ64_04160"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNC92716.1"
                     /translation="MSHDHDHSEEGHSHGIGSYRGADRKALLIAALLTGGFMVAEAVG
                     GVITGSLALLADAGHMLSDSFSLFLALFAVSIAARPATAKRTFGYKRAEILAALVNGV
                     LLVLVSLWVVYEAIGRLDDPVEVMGGGMMAIAVLGLIINVVAFLVLWKGGGESLNVRA
                     ALRHVLGDLLGSVGVIIAAGVILLTGWEPIDPIVSILISILIVASAWSVLKESVDVLL
                     EAAPKGIDTQEVGMAMAGVAGVEQIHDLHIWQITSGFPSLSAHVLVGADLDCHRVRLE
                     LDSLLKEQFEIEHSTLQLEHAGDGKPLEITRPDGLTASS"
     gene            complement(4056..4697)
                     /locus_tag="PLJ64_04165"
     CDS             complement(4056..4697)
                     /locus_tag="PLJ64_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92717.1"
                     /translation="MAPRFPVVLEPEEWDLFQRPGSVEILRAHGRSTSPDLTDAQIIC
                     LIPHLAPFLLRKRIVEMIGAGWLDTSFPLSLDLHMEAYEKSVYRRKSSLLPRGARLAD
                     VAIKLVEASPDAKPVELVQEPGREIRDNIKDPYFVFQQYDLPLEFSIDRHFSNLLEKL
                     SEEEIRASLREIRENLIAREPERQIRKDPGRWARSVARLRNLPGLVRPNRERI"
     gene            complement(4734..5078)
                     /locus_tag="PLJ64_04170"
     CDS             complement(4734..5078)
                     /locus_tag="PLJ64_04170"
                     /inference="COORDINATES: protein motif:HMM:NF013777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HNC92718.1"
                     /translation="MVASDNRQLRRFDEPEIWRVFSRRGTIQILLALSRRDAMRSRDI
                     DAEFPSIARQVLGTRLNELRELGAVQRRVFDGPPMTSEYSLTDLGREISVAANVLENA
                     ARKSGELEANAA"
     gene            complement(5167..5523)
                     /locus_tag="PLJ64_04175"
     CDS             complement(5167..5523)
                     /locus_tag="PLJ64_04175"
                     /inference="COORDINATES: protein motif:HMM:NF013777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HNC92719.1"
                     /translation="MEKPRAAQRPDEPEEWRVFQRRGTPQILLLLSDGSSLRSSEIDA
                     AIPTIKRQILGTRLNELRELGILEREVEEGPPMISRYRVTEKGTALAEVAEALNRVVS
                     ATPLAEEDAGVDVPRP"
     gene            complement(5649..5987)
                     /locus_tag="PLJ64_04180"
     CDS             complement(5649..5987)
                     /locus_tag="PLJ64_04180"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HNC92720.1"
                     /translation="MAGVNKAQRALLDEAVAVAGRSRELFPYGGFEDLDGSEIQILLC
                     VVGSPGDSVLAISKKLRFEQPTMSRPLSKLVDKHLIEVAVDPNDGRRKLIKPTRSGVK
                     ITERYLKSVA"
     gene            6170..7924
                     /locus_tag="PLJ64_04185"
     CDS             6170..7924
                     /locus_tag="PLJ64_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92721.1"
                     /translation="MTQHNKKTRETQLSRRLRRAINQEAHELIKKDPNLGRVKALKTA
                     RQIVIARERKNPHRKRIRELTEPELRAVKRSETCSVAEQRAIRKIPAIQWLVAYLLTA
                     GRTRPFLNRLLVTAALFEMAFTVRPEFRFVFSRFRSGDSINWAHGSPQGPFTIEANYH
                     ALRKVTDCGKSGATIHINLDLLDQIAATKVAGELRFPNFGRFCAIDGTLIEANVPQTL
                     PRMRSPRARKTLIREIAGKHRRMADMVFHGFGKHSDAIDDQPEPGLTIRKRCFGYNLV
                     VISCLDTCLPVIWTLMPATGNEVAAARELIEALFELRPDFPMEYLVGDGLYNRGFEFP
                     STLYKNYGIHLVSPRTNRKSKSEWADSDGVPRCAHGLMKFTRHGEVWDAAKRRAHGIR
                     PGNLPPNGKGLNPRLRFECHEEVCREKSTRMEDDWILHTFLPHIGQDKWTALREVLLA
                     RRNTSESLFAQLKQLGVGAKWPNRARWATDNGMRWLTSLALLNMTARKLVHLDGSYAA
                     ALKDAYSAGIITRQQMHRFLEVEGIETDSRNRVDDTSPVEPSTWNDWVELPIDREFLA
                     TPVDAYSDIPYGSRKSEK"
     gene            complement(8173..8245)
                     /locus_tag="PLJ64_04190"
     tRNA            complement(8173..8245)
                     /locus_tag="PLJ64_04190"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8210..8212),aa:Ala,seq:cgc)
     gene            8356..9357
                     /locus_tag="PLJ64_04195"
     CDS             8356..9357
                     /locus_tag="PLJ64_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92722.1"
                     /translation="MGILDEAIREHLELKRQHGADESELQGLEDEAFGEAERPDAVSP
                     SPGSEDQTQVISPGSSVTSPAGEGDQLDKVFEATRQREADAEAAVAREAAKAKGESTE
                     TPAPVDPEATFAEHPVPEPPAPAEAAGEAATVESPEAKPTEPEAAVEPPAPAQEGDAG
                     DMLEAERHHLASHPTEHYDVDAAIAEEDEIDVLSESSLSDELDRALESGETAVAGGET
                     VEYDMSSEPDSGEFEFDEPDSAEMESAEHEAEPESAGQEAVEGDSAELLYQDGATEDQ
                     PVDQDEPETGEEDAEGSSEFYDQNDVLEGTPDFLEETPEHDQLWFEQKEPKDFDFGD"
     gene            complement(9387..10241)
                     /locus_tag="PLJ64_04200"
     CDS             complement(9387..10241)
                     /locus_tag="PLJ64_04200"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC92723.1"
                     /translation="MAIDDLASTLDHRDNRATGESVVRARGVNVFRWSTALRQRVMLL
                     EEIEWRVDRGQHWVMLGPNGAGKTTLLHLAAADSHPSEGTVEVLGKRLGNTDMRALRE
                     AIGLVDARTSRAIPSRRPVLETIESGAFNSIALQHQRLDASHRDRARELMAVVGLSDL
                     ATRAFGECSQGERQRILLARALMPSPELLLLDEPAAGMDLPSREQLVSALVSMAEGDP
                     GLTTVTITHHLEEIPPTATHALLIDHGRIVAQGPIDEALTSESVSAGFGLPIDVRKAS
                     GRWTAAIS"
     gene            10305..10377
                     /locus_tag="PLJ64_04205"
     tRNA            10305..10377
                     /locus_tag="PLJ64_04205"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10338..10340,aa:Ala,seq:tgc)
     gene            10522..12879
                     /locus_tag="PLJ64_04210"
     CDS             10522..12879
                     /locus_tag="PLJ64_04210"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase"
                     /protein_id="HNC92724.1"
                     /translation="MDTFLEDRIDPEEVDEWVQTASILHSNGDGLDIAVEGGRIVGVR
                     GREDDRVNHGRVDPKDLYGWQANNSPDRLTRPLIRRDGELVECDWDTAMEALVARSTE
                     LLESGPAGWGHFGFYTSGQLFLEAYYTLGVIGKAGIGTPHMDGNTRLCTATAAASLKA
                     SFGADGQPGSYTDVDHCDAIALYGHNVAETQTVLWMRMLDRRAGPDPPRMLAVDPRPT
                     PVAREADIHLAVRSGTNLALTNGLARGLIKRGWIDRDYIAGHTLGFDRLEATVEPYDP
                     KTVAGICGIEARDVEAATELIGTSERLLSTVLQGFYQSNQATAAACGVNNIHLMRGML
                     GRPGCGLYQMNGQPTAQNTRETGADGDLPALRNWENKEHIQELADLWNVDPLKIPHWA
                     APTHAMKIFRYAEQGSIKLLWVSATNPAVSLPDLNRVRRTLAKEDLFLVVQDIFRSET
                     AEFADLVLPAATWGEKTGTFTSVDRTVHLSEKAVEPPGEARSDLEIFLEYARRMDFRD
                     KDGDPLIKWTDPEGAFEAWKDCSRGRPCDYSGMSYERLREGPIQWPCNEANPDGTERL
                     YTDHTFSTRPDYSETFGHDLATGASLSEEEFRAKEPDGRAFLHAVPYEPSPEVTTPEH
                     PYLLTTGRTVYHFHTRTKTGRAPQLNDAAPDVWVELHPEDAAELEIEEGDLVRIASPR
                     GAIDAPARLNGIRRGSVFLPFHYGSWDREDEEGRAANNLTVTAWDPVSKQPIFKVAAV
                     SLQRVGAANGTPSPAPTTTASAPVAERVPATVGGPSAESFCEELP"
     gene            12876..13334
                     /locus_tag="PLJ64_04215"
     CDS             12876..13334
                     /locus_tag="PLJ64_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92725.1"
                     /translation="MNLGHYLSLISRSQHELAEAFREVAAGHAADAAVFHISGQLAAQ
                     CDRHVGELKPFVERYGVEGEEPDRLHSDLFQGTREGPLGMLRDLHDLYLMAAEVDMSW
                     TVIAQAAQGVRDRDLLAVTSQCEKETATQMKWIRAQMKETAPQTLVVASG"
     gene            13365..14825
                     /locus_tag="PLJ64_04220"
     CDS             13365..14825
                     /locus_tag="PLJ64_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92726.1"
                     /translation="MSGPEPEALQQWANSRGLAWQPEDTLPPVTERLRAGVGVGEHRA
                     SWRTVSDRGMTTITGSNSKKPERQTVGVCEGTLPGGLNGVIGHHFHQIDQGSGQEDRY
                     LAITDTVVFAELPAGARPVFHLEGSKGGGEVKGAFAVGNSGEPASPLDGVVPAPSGTI
                     EAGGLRWVSFPAEEDERIRGAVSAATRSLDGIPWERAVVEYESGRLAVWVRDRPLTDA
                     ASLDALCSFASAVADGLNEAAGSAPPLDLRAPLAMPEPGAREKWVSAGADLVVWEEPP
                     VSIIAAQERYKKDVRPQATRTGWKVYGIVGVSLFIFSLLVAGASLAISWAVDELAVGI
                     VVAVASVVLGIFGATRIGLETGQEALDDRLKSSAIPWGIEAFARGYAKHAGLTREDHE
                     ELRRRLAVPFRGRCQIAWQGELAPGRTGHLSIWIDATGTPEPPRFFLLAVAGNNGAGA
                     PAGYESSEVDGLRLTWQEVTSVQRADHRLDQLRAVA"
     gene            14898..>15429
                     /locus_tag="PLJ64_04225"
     CDS             14898..>15429
                     /locus_tag="PLJ64_04225"
                     /inference="COORDINATES: protein motif:HMM:NF025050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNC92727.1"
                     /translation="MSRIFGKVAQNGYVVKDIRSEMDHWVGLGVGPWFYMGRVEMDWF
                     RHRGEDSEPPEVSIALANSGDLQIELIQQRNDAPSMYREFIDAGHEGLQHMSYWSEDY
                     QSLYDKVLGLGFEIGHEGCIGGEQGRFAYLDTQTTPGTVIEISDVSGAKGMFFEHVRS
                     EAENWDGSDPIRELIPD"
BASE COUNT         3042 a         4872 c         4830 g         2685 t
ORIGIN      
        1 ttcgtggtca agtccgatgt tgatgacgag gtccacgtcc acggattcga tgtcagcgaa
       61 cccgtaggtc ccggcaaacc ggccaagttt gacttcaaag ccggcttcac cggagtcttc
      121 gaaatcgaac tcgagaactc agccgtcccg atcatcgagc tccaggtcaa cccttgatca
      181 gtctgcccct cgcgcatgcc ctggtctcgc ggggtgacct gccgattcct gtttggcttt
      241 tcgcgtgggg cgccggcgta gtccttgttg tctcattttt tgcgctggcc tcggcctggc
      301 gcaaacccag attcgaagat gctccgggtc gacccgcagc gacctggctt tcagcgacag
      361 caaactcttc ggccgttcag ttcctctcag gtctgatcgg cgtccttcta cttgcggttg
      421 tcctctacgc ggggatcgaa ggaaccgaag ctgccgaccg aaacttctct ctcaccttcg
      481 tcttcgtaac cgtctggctc ggcttccccg tcctgagtgt ggtcttcggg aacgtcttcg
      541 ccgcgttctc tccgtggagg gcaatcggca gggcttatgg atggcttgcg cagcgactgg
      601 cgccgggagc cgtaaggcac gtcgagtacc cggagaaggt cggcaggtgg ccggcggcgg
      661 tcggactcgt cgccttcgtc tggctcgaga tcatctacgg agcgacgggc gcggccgtgg
      721 ttctctcccc cgaaatcgtg gccaaggcga cggtcatcta ctcggtctac accctggtga
      781 tgatgggcgt gttcggcgtt gagaaatgga gctcacgtgg agagttcttc tcggtctact
      841 tcggcatgtt cgcctcgatg tcgtcgtttc gaaagcggga aggaaagctc gagcgtcgac
      901 gttggctctc gggaaccacc gattgggcaa acgtgccggg atcgctggcg ctcgtcgttt
      961 ctgccatcgg tgtgaccacc ttcgacggtg cgcaggaagg tgcgctgaaa accccgatcg
     1021 agtggacgca ggaacgaatc ggtgacctgg ggttcgcggc aacagtctcg gtccgggctg
     1081 gcgacaccat cttcctgctg atcacgatcg gaatcgtggc actcatctac ctcctcggag
     1141 tcaagggaat gacccgggtg gatgcggcgc caccgttcaa ggtgcttcgt agccgctttg
     1201 ctcacacact cattccgatc gcgctggcat acctcgtggc ccactacttc agtctcttcg
     1261 tgttccagga gcaagcccag ttcacctttc tgctctcaga tccgctgggc gacggatcga
     1321 atctgttcgg gacagcgaca tcagggatcg actacaccct gatcgggccc gaacttgtct
     1381 ggtacgtcca ggtcggggca ctcatcgccg gccacgtgac tgcgctcgtc ctcgctcatg
     1441 accgcgcact tctgatctgg ccggactacc gactggcgac cagatcccag tactggatgc
     1501 tcgcggtcat gctctgcttt acgagtctgg gcctcttcct gctctcattt gcgaaccagt
     1561 agaacgggcg cttcattgca gggctacacc tcgccggctc aacccaagat ctaactacta
     1621 cagtccgtag gagcttggct ccccttccct cgaaactcac ttctccactc gaaccgtgga
     1681 gacgacacat tcccctgctc gccgcttccg cctgcctgct cctcgtggtg ctcatgtcgg
     1741 gaagttcatc ggcagccgac acgcaatcga agctggacaa ggcagagaag aagatcgaag
     1801 aaggccaaca gcgggaggga gtcctgaccg agaaacttga tcagctcggt gagcgggtcg
     1861 actcaatgca ggccgcggtc gacgcactgc gagaggccga acaagaagcg gcggcgaaac
     1921 tccagcaggc agaagctgac ttgaaactcg cgatcgaacg actccagact tccctcgaag
     1981 agctccgcga aactcgaaga cgtctgaagg tgtcccttca ggtcctcaga cagaggttgg
     2041 tggacatcta cgtctcaggt acaccaagca ttgcctcgat ggtgctggcc gccaacgagt
     2101 acagcgatct ggtcgagatg ggaccgtacc tcgaagcgat ccagtcgcga gacgagtctc
     2161 tcgttcagag agtcagggaa ctgaagaacg aggcgcgcaa gatcgtggag gcccgacgcg
     2221 actcaaagga aaccatccaa acttctcgcg actcgatcgc cgccgagacc gcgcgattgg
     2281 cgagtgctcg aagtgaaacc gaatcccagg tcgcatctct tgcgtcggcg aaggccgcga
     2341 gcgaagaggc actgaacgag gttcaggacg gaatcgctca ggaagaggaa gtcgcggcgg
     2401 acctgcgcga gaagctcacc aaggagctgg gtacagccac gtcggtcccg ttctccgtgg
     2461 gaccggcgtc aacacccagt tccaacagca tgatctggcc gatcgacgga actcttactt
     2521 cgccgttcgg cccaagatgg ggcagaacgc atgaaggcct ggacatcgcg gcccccgccg
     2581 gcacaccaat cgccgccgcg gcatcgggga cggtagtgct catgcagtca gaagcagaga
     2641 gcggcggcta cgggctcttc acctgcgtgg atcatggcgg tgggttgtcc acctgctacg
     2701 cccatcagtc cgaattcgga acatcggtcg gggcatctgt caagcagggg gaagtgatcg
     2761 ggtacgtcgg aaacacaggc aacagctttg gtgatcacgt tcacttcgaa gtacggatca
     2821 atggggcggc aaccgacccg atggcttacc tctagcttga ggcggtcaag ccatccggcc
     2881 gggtgatctc cagaggcttc ccatcgcccg cgtgctcaag ttgaagcgtt gagtgctcga
     2941 tctcgaactg ctctttgagc aacgagtcca actcgagtct gactcgatgg caatcaaggt
     3001 cggcgccaac cagcacgtga gctgagagcg agggaaagcc ggaagtgatc tgccagatgt
     3061 gcagatcgtg gatttgctca actcccgcga ctccggccat ggccatacct acttcctggg
     3121 tatcgattcc cttgggggcg gcctcgagca agacatccac cgactccttc aagactgacc
     3181 aggcgctcgc cacgatgagg atggagatca ggatcgacac gatgggatcg atcggctccc
     3241 agcccgtcaa aaggatgacg ccggcggcaa tgatcacgcc gacggaaccg agcaggtcac
     3301 ccaagacgtg ccgaagggca gctcgaacgt tcagactctc accgcctccc ttccaaagga
     3361 ccaggaaggc aaccacgttg atgatgaggc cgaggacggc gatcgccatc attccgccgc
     3421 ccatcacttc aacgggatcg tcgagtcttc caatcgcttc gtacaccacc caaagggaca
     3481 ccaagacgag aagcacgccg ttgaccaaag ccgccagaat ctcggcgcgc ttgtatccga
     3541 aggtccgctt cgctgtcgcg ggacgggcgg caatcgatac agcgaacagg gcgagaaaga
     3601 gcgagaagct gtcggagagc atgtggcccg cgtcagcaag tagcgcgagc gaacccgtga
     3661 tgactccgcc gacagcctcg gcgaccatga agccacccgt caacaaagcg gcgatcagaa
     3721 gcgccttacg gtcagctccc cgataggacc cgatcccgtg gctgtgaccc tcttcggagt
     3781 gatcgtggtc gtggctcatc tagacgcgat tctatcgacc tactacagca cgtcgcagat
     3841 cgggcgactt cgatgattcg agcctctata cgactttatg tggtcgtata cggaaaagcg
     3901 cataaagctc cgccacaagg gatcggtcac ccattgatga ctcgatcagg cgaattcaaa
     3961 tctaagggtg tctacggctg tctacggatt gtccgtagac aaaccgatga ggctggcttt
     4021 gttcagacgt cggttcagct cgacctgcga tacgactaga ttcgctcgcg gtttggtcgg
     4081 accaatcccg ggagatttct cagacgggcc acgctcctgg cccaccgtcc ggggtctttc
     4141 cggatttgcc tttcgggctc gcgggcgatc aggttctccc tgatctcgcg aaggctggct
     4201 cgaatctcct cttcactcaa cttctcgagc aggttcgaga aatgacggtc aattgagaac
     4261 tccagtggca agtcgtactg ctggaaaacg aagtagggat ccttgatgtt gtcgcgaatc
     4321 tcgcggcccg gttcctggac gagctcaact ggcttcgcgt cgggagaagc ctcaacgagc
     4381 ttgatcgcga cgtcagcgag cctcgcgccg cgagggagca gactcgactt tcgccgatat
     4441 acagatttct cgtacgcctc catgtggagg tcgagactca acgggaagct cgtatcgagc
     4501 caacctgcac caatcatttc cacaatgcgc ttcctcaata agaaaggggc caggtgcgga
     4561 atcagacaga tgatttgcgc atccgtcaga tcgggcgaag tggatctccc gtgcgctcga
     4621 aggatctcga cggaacccgg tcgctggaac agatcccact cttcgggctc gaggacaacc
     4681 ggaaatcgtg gcgccatctg tcgagattat gacgcggact cggtcttgtc taactatgcc
     4741 gcgttggctt cgagctcacc cgacttacgg gcggcattct caagcacgtt tgcggcgacg
     4801 gagatctcgc ggcccaggtc ggtgaggctg tactcggacg tcatgggtgg cccgtcaaac
     4861 acgcgtcgct gaacggctcc cagttcccgc agttcgttca aacgagtccc gagcacctgt
     4921 cgggcaatcg acgggaactc agcatcgatg tcccttgatc tcatggcatc tcttctggag
     4981 agagctagaa ggatctgaat ggttccgcgg cgcgagaaaa cgcgccagat ctcgggctcg
     5041 tcgaatcggc gaagttgtcg attatcgcta gccaccatca tcaaaggata cgccctatca
     5101 gtggctgaag ttcgagatag caatgccgcg atttagttcg aactgagaag tgaaatctgg
     5161 aggaagctag ggccggggaa cgtcaacgcc ggcgtcttct tctgcgagcg gcgtggcgct
     5221 gacgacgcgg ttcagcgcct cagcgacttc tgccaaagct gtccccttct cggtcacgcg
     5281 atatctggaa atcatcgggg gaccttcttc tacctctcgt tcgaggatcc cgagttccct
     5341 tagttcgttg agcctggttc ccagaatctg gcgcttgata gttggtatcg ccgcgtcgat
     5401 ctcgctgctt ctcagcgacg aaccatcgct gagcaatagg agaatttgag gcgttccacg
     5461 cctctggaac acacgccact cctctggctc gtcgggtcgc tgggctgctc gaggtttttc
     5521 catccgaaag ggctattacg ttctcagcgt gccactaatt aggggcagag ctactattga
     5581 ctggcgggac tcgatacgtc tcatcagcaa tctacaagtg atccgatcgc taaagccagg
     5641 ggttgcgatc aagcgactga cttcaggtac cgctctgtga tcttgacacc ggagcgcgtt
     5701 ggcttgatca gcttgcgtcg cccgtcgttt gggtctaccg cgacttcgat cagatgcttg
     5761 tcgaccagtt tcgaaagcgg tcggctcatc gttggttgtt cgaaacgaag cttcttagag
     5821 attgccagca ccgagtcacc gggtgatccg actacgcaaa gcaagatctg gatttcagaa
     5881 ccgtccaggt cctcgaaacc cccatacggg aacagctccc ttgatcggcc ggcaacagcg
     5941 acggcttcgt ccagcagggc tcgttgggcc ttgttcacgc ccgccacccg ttgcaacaag
     6001 aatttcgcgg gtacttctca atccgaagta gtttcggcaa cgccgttcct ccaacaggtt
     6061 cggtcgatgg gaggtgaagc cacgccagaa ctctgagaaa cagatcgcga agtgtttctg
     6121 aactcactga tgcaccacgg ctcatgccgc ggcccacaga ggagaaatga tgactcagca
     6181 caacaagaag acgagggaaa cgcagctcag caggcgcctc cggcgagcga tcaaccagga
     6241 ggctcacgag ctcatcaaga aggacccaaa cctgggaaga gtcaaagccc tcaagacagc
     6301 tcgtcagatc gtcatcgcga gagagaggaa gaacccgcac cgcaaacgaa tccgggaact
     6361 cacagagccc gagctcagag ccgtcaagag atctgagact tgctcggtcg ctgagcagcg
     6421 agccatcaga aaaattccgg ccattcagtg gctcgttgcc tatctcctga ccgcgggaag
     6481 gacccgtccg ttccttaacc gattgcttgt cacagcggct cttttcgaga tggcgttcac
     6541 ggtccgtcca gagttccggt tcgtgttctc cagattccga agtggcgact cgatcaactg
     6601 ggcgcatgga agtccccaag gcccgttcac gatcgaagcc aactaccacg ccctgcggaa
     6661 agtcactgac tgtggaaagt cgggggcgac cattcacatc aacctggatc tcctcgacca
     6721 gatcgccgcg acgaaggtag ccggcgaact ccggttcccc aacttcggtc ggttctgtgc
     6781 catcgacgga acactgatcg aggcgaacgt tccccagaca ctcccaagga tgaggtcccc
     6841 tcgcgccagg aagactctga tccgcgagat cgcgggtaag catcggcgaa tggccgacat
     6901 ggtcttccac ggcttcggaa agcactccga cgccatcgac gatcagcccg aacccggtct
     6961 cacaattcgg aagcgttgct tcggctacaa cctcgtcgtc atcagctgtc tcgacacctg
     7021 cctgccggtg atctggacgc ttatgccggc aacaggaaac gaggtcgccg ccgcgaggga
     7081 actcatcgag gctcttttcg agctgcggcc tgacttcccg atggagtacc tggtcgggga
     7141 tggcctctac aacaggggct tcgaatttcc gagcaccctc tacaagaact acggcatcca
     7201 cctggtctct ccaagaacca acaggaagtc gaagagcgaa tgggccgaca gcgacggtgt
     7261 gccccggtgc gcacacggcc tcatgaagtt cacccgacat ggcgaggtct gggatgccgc
     7321 gaagcggaga gctcatggca ttcgcccggg aaaccttcct ccaaacggga agggcctgaa
     7381 cccgcggctt cggttcgagt gccacgagga agtctgccga gagaagagca cccgcatgga
     7441 ggacgattgg atcctccaca ccttcctgcc ccatatcggg caggacaaat ggaccgcact
     7501 cagggaagtc ctgctggcga ggcgcaacac atccgagtcc ctcttcgctc agctcaagca
     7561 actcggcgtc ggagcaaagt ggccgaaccg agccaggtgg gccaccgaca acggcatgcg
     7621 ctggctcact tcgctggccc tcctgaacat gacggcccgc aagctcgtcc acctcgacgg
     7681 gagctacgcc gccgccctca aggacgccta cagcgccggc atcatcaccc ggcagcagat
     7741 gcaccgattc ctcgaggttg agggcatcga aacggactct cgaaaccgag tggacgacac
     7801 ctccccggtc gaaccgtcta cctggaacga ctgggtcgaa ctcccgatcg atcgagagtt
     7861 ccttgcgacc cccgtggacg cctacagcga catcccctac ggctccagaa agtcggagaa
     7921 gtagaccccc tctacatcta gttgttagtg cgcggccgcc tctgggcggc cgcgttctat
     7981 tgcgaccacc ccgattttcg gggcggtgaa tttggcttcc cggctgggtt tcttgcgcga
     8041 ccaattttgg gcgagatcaa ggcaaaacgg ggtcaacttc tttgagtgag atttcggggg
     8101 ttccgaaagc cactccgcaa gccaaatatt gagttgtgag atttttagag tcatttcttc
     8161 agtgactcgt agtggagcta gccggagtcg aaccggcgac ctcttgggtg cgatccaagc
     8221 gctctgccaa ctgagctata gccccgcggg gaaacaagga tacggaagct cggcgtgcaa
     8281 agtagggaaa gctcatgcac gacgtccgcg aaaaccgcat aatcttaagc cttaccgtta
     8341 gctactgaga gaccaatggg aattctcgac gaggcaatcc gggaacacct cgagctgaag
     8401 cgacagcacg gggccgacga atccgaactt cagggactcg aagacgaggc cttcggcgag
     8461 gccgaacggc ccgacgccgt aagtccctcc cccgggtcgg aggaccagac ccaggtcatc
     8521 agccctggtt ccagcgtcac ctctccggcc ggtgaaggcg accagctcga caaggtcttc
     8581 gaagcaaccc gccagcggga agccgacgcc gaggctgcgg tcgccaggga agcagccaag
     8641 gccaagggcg aaagcacgga aaccccggca ccggttgatc cggaagcgac tttcgccgag
     8701 cacccggttc cggaaccgcc ggcgccggcc gaggcggctg gcgaagcggc gacggttgaa
     8761 tcacccgagg cgaaaccgac cgaacccgaa gcggcggtcg agccgccggc tcccgcccag
     8821 gagggcgatg ccggggacat gctcgaggcc gaacgccacc accttgccag ccatccgacc
     8881 gagcattacg acgtcgatgc cgcgatcgcc gaggaggacg agatcgacgt gctcagcgag
     8941 agttcactct cggatgaact cgaccgggcc ctggaaagcg gcgaaaccgc cgtggccggg
     9001 ggcgagaccg tcgagtacga catgtcatcg gaaccggact cgggcgagtt cgagttcgat
     9061 gaacccgact cggccgaaat ggaatcagcc gaacatgaag cggagccgga atcggccggg
     9121 caggaagcgg tcgaagggga ttcggccgag ctgctctacc aggatggcgc caccgaggat
     9181 cagccggtcg atcaggacga gccggaaacc ggtgaagaag acgccgaggg ttcgagcgag
     9241 ttctacgatc agaacgatgt gctcgagggc accccggact ttctcgaaga aacgcccgag
     9301 cacgaccagc tctggttcga gcagaaggaa ccgaaggact tcgacttcgg cgactgagct
     9361 ggttctgccg gtcgccgtcg aaccagtcag cttatggccg ccgtccagcg gcctgacgct
     9421 ttgcgaacat cgatcggcag gccgaaaccg gcagacactg attcagaggt gagtgcctcg
     9481 tcgatcgggc cctgggcgac gatccggccg tgatcgatca gcagtgcgtg ggtggcggtc
     9541 ggcgggatct cttccaggtg gtgggtgatg gtgaccgtgg tcaggccggg gtctccttca
     9601 gccatcgaga ccagcgcgga aacgagctgc tcgcgcgagg gcagatccat gccggcagcc
     9661 ggctcgtcga ggagcagcag ctcagggctc ggcattagcg cccgggcgag caggatccgc
     9721 tgccgctccc cctgcgagca ctcgccgaag gcccgggtgg cgaggtcgct caaaccgacg
     9781 acggccatca attcccgggc ccggtcgcgg tgggaagcgt ccaggcgctg gtgctggaga
     9841 gcgatgctgt tgaaagctcc ggactcgatc gtctcgagga ccgggcggcg ggaggggatc
     9901 gcccgcgagg tgcgggcgtc aaccagtccg atggcttccc tcagcgcccg catgtcggtg
     9961 ttgcccagcc gcttgccgag cacttcgacc gtaccttcgg aagggtggga atcggcggcg
    10021 gccaggtgga ggagggtcgt cttgccggcg ccgttcgggc cgagcatcac ccagtgctgg
    10081 ccgcggtcca cgcgccactc gatttcctcc agcagcatca cccgctgtcg aagtgcggtt
    10141 gaccagcgaa agacgttcac ccccctggcc cggaccacgg attcgcccgt tgcccggttg
    10201 tcccggtgat cgagggttga ggcaaggtcg tcgatcgcca ccggctcagg ctatctgcgg
    10261 cagggtccgg ccgcccccga acccgtacaa tcgccgtcca ctctggggcc atagctcagt
    10321 tggcagagcg cctgctttgc aagcaggagg tcgccggttc gatcccggct ggctccactc
    10381 gcgtgaccgg taacgggttg cggaaggtcg gcgtgattgg cgcgctgtcg cgtgggtacc
    10441 tgatctctca tggtcgaccg gatcgcggat ccctggggga aaaggactcc ctacggaagg
    10501 ggggaggcgt ggccgcgccg ggtggacacc tttctcgagg accggatcga cccggaagaa
    10561 gtggacgaat gggtgcagac cgcctcgatc cttcactcga atggtgacgg gctcgatatc
    10621 gcggtcgagg gcgggcgaat cgtcggagtc cgcggacgcg aggacgaccg ggtcaaccac
    10681 ggccgggtcg atccgaaaga cctgtacggc tggcaggcca acaacagtcc cgaccggctg
    10741 acacggccgc tgatccgccg ggacggtgaa ctcgtcgaat gcgactggga caccgccatg
    10801 gaagccctgg tcgcccgctc gaccgagttg ctggaatccg gacccgccgg ctggggccac
    10861 ttcgggttct acacgtccgg ccagctcttc cttgaggcct attacacgct cggcgtgatc
    10921 ggcaaggccg gtatcggcac tccgcacatg gacggcaaca cccgcctgtg caccgccacc
    10981 gcagcggcct cgctcaaggc gagcttcggc gccgacgggc agcccggttc ctacacggac
    11041 gtcgaccact gcgacgccat agccctctac ggacacaacg tggccgagac ccagaccgtt
    11101 ctctggatgc gaatgctgga ccgccgggcc gggccggacc cgcctcggat gctggccgtg
    11161 gacccgcgac cgacccccgt tgcccgcgag gcggacatcc acctggcggt gaggagcggt
    11221 accaacctgg ccctgaccaa cggcctggcc cgcgggctga tcaaacgggg atggatcgac
    11281 cgggattaca tcgccgggca cacgctgggc ttcgaccgtc tcgaagcgac agtggagcct
    11341 tacgacccga agacggtggc cggcatctgc ggaatcgaag cccgcgatgt cgaggcggcg
    11401 accgaactga tcggcacttc cgagcgactg ctctccactg tccttcaggg cttctaccag
    11461 tcgaaccagg cgaccgcggc cgcctgcggg gtcaacaaca tccacctgat gcgcgggatg
    11521 ctcggccggc cgggatgtgg cctctaccag atgaacggac agccgaccgc gcagaacacg
    11581 cgcgagaccg gcgccgacgg cgacctgccc gccctgcgca actgggaaaa caaggaacac
    11641 atccaggaac tggccgacct ctggaacgtg gacccgctga agatcccgca ctgggcggca
    11701 ccgacccacg cgatgaagat ctttcgctac gcggagcagg gctcgatcaa gctgctctgg
    11761 gtcagcgcca ccaatcccgc ggtttccctg cccgacctga accgggtgcg ccggaccctt
    11821 gcgaaggagg atctgttcct ggtggtccag gacatcttcc ggagcgaaac cgccgagttc
    11881 gccgatctgg tcctgcccgc cgctacctgg ggcgagaaga ccggcacctt caccagcgtc
    11941 gaccggaccg tccacctgtc cgagaaggcg gtcgaaccgc cgggcgaggc gaggtcggac
    12001 ctcgagatct tcctcgaata cgcacggcgg atggacttcc gggacaagga cggcgatccg
    12061 ctgatcaagt ggaccgaccc ggaaggcgcc ttcgaagcct ggaaggactg ttcgcgcgga
    12121 cggccctgcg actactcggg aatgagctac gaaaggctgc gcgagggtcc gatccagtgg
    12181 ccctgcaacg aagccaaccc ggacggcacc gaacgcctct acaccgacca caccttttcg
    12241 acccggcccg attactcgga aaccttcggc cacgaccttg cgaccggtgc cagcctgagt
    12301 gaagaggagt tccgggcgaa ggagccggac ggacgcgcct tccttcacgc cgtcccctac
    12361 gagccttcgc ccgaagtcac cactccggag cacccgtacc tgctcaccac cggtcgaacc
    12421 gtctaccact tccacacccg gaccaagacc gggcgcgcac cgcaactgaa cgacgccgcg
    12481 cctgatgtct gggtcgagct tcaccccgaa gatgcggcgg aactcgagat tgaagaaggc
    12541 gacctggtcc gcatcgcctc gccgcggggc gcgatcgacg cgccggcccg gctcaacggg
    12601 atccgccggg gttcggtctt tttgccgttc cattacggaa gctgggaccg cgaagacgag
    12661 gagggccggg ccgccaacaa cctcaccgtc accgcctggg accccgtttc caaacagccg
    12721 atcttcaagg tcgccgcggt cagccttcag cgggtcggtg ccgccaacgg gaccccgtcc
    12781 ccggcgccga ccactaccgc ctcggctccg gtcgccgaga gggtgccggc gacggtcggc
    12841 gggccttcgg ccgaatcgtt ttgcgaggag ctcccgtgaa cctcggccac tacctctccc
    12901 tgatcagccg gtcgcagcat gagctcgccg aggcgtttcg cgaagtcgcc gccggtcacg
    12961 ccgccgacgc ggcggtgttt cacatctcgg gtcagctcgc cgcccaatgc gaccggcacg
    13021 tgggcgagct gaagccgttc gtcgaacgct acggcgtcga aggcgaagaa ccggaccgcc
    13081 tgcacagcga cctgttccag ggaacccgcg agggtcccct cggcatgctt cgcgaccttc
    13141 acgatctcta cctgatggcg gcggaagtcg acatgtcatg gaccgtgatc gcacaggccg
    13201 cgcagggtgt tcgcgaccgg gacctgctcg ccgtgaccag ccagtgcgag aaggagaccg
    13261 cgacccagat gaaatggatc cgggcgcaga tgaaggaaac cgccccgcag accctggtcg
    13321 tggcgagcgg ttagaggccg gtcctgcggc taggatccgc gccgatgagc ggaccggaac
    13381 ctgaagcgct tcagcaatgg gcaaacagca gagggctggc gtggcagccg gaggacacgc
    13441 tgccgccggt caccgagcgg ctgcgggccg gggtcggggt cggggagcac cgtgcctcct
    13501 ggcggacggt ctcggaccgg ggcatgacca cgatcaccgg ttccaacagc aagaagccgg
    13561 aacggcagac ggttggcgtc tgcgagggca ctcttcccgg cggtttgaac ggggtgatcg
    13621 gccaccactt ccatcagatc gaccagggct ccggccagga ggaccgctac ttggcgatca
    13681 ccgacaccgt cgtctttgcc gagctcccgg ccggggcgcg gcccgtcttt catctcgaag
    13741 gcagcaaggg aggcggagag gtcaagggcg ccttcgcggt cgggaactcc ggcgaacccg
    13801 cgagcccgct cgacggtgtg gttccggcgc catccggcac gatcgaagcc ggcggccttc
    13861 gctgggtcag cttcccggcc gaggaagacg agcgcatccg cggtgccgtt tccgccgcga
    13921 cccgttcgct cgatggcatc ccgtgggaga gggcggtagt cgagtacgaa agcggccggc
    13981 tcgcggtctg ggtcagggac cggcccctca ccgacgcagc ctcgctcgac gccctctgca
    14041 gcttcgcctc cgccgtggcg gacggcctca acgaggcagc cgggtcggcc ccgccgcttg
    14101 acctccgggc acccctcgcg atgccggagc cgggcgcgcg ggaaaagtgg gtttccgccg
    14161 gcgccgacct ggtcgtttgg gaggagccac cggtcagcat catcgccgcc caggagcgct
    14221 acaagaaaga tgtcaggccg caggcgacca ggaccgggtg gaaggtctac gggatcgttg
    14281 gagtcagcct tttcatcttc agcctgctcg tcgccggcgc gagtctggcg atcagctggg
    14341 cggtcgacga gctggcggtc gggatcgtcg tggcggtcgc ctcggttgtg ctggggatct
    14401 tcggagcgac ccggatcggc ctcgaaaccg gccaggaggc gctcgatgat cgcctcaagt
    14461 cgagcgccat accgtggggg atcgaagcct tcgcccgggg ctacgcgaaa cacgccgggc
    14521 tgacccgcga agaccacgag gagcttcgcc gccggcttgc ggttccgttt cgcggccgct
    14581 gccagatcgc ctggcagggc gagctcgcac cgggacggac cggccacctc tcgatctgga
    14641 tcgacgcgac gggcacaccg gagccgccgc gcttcttcct gctcgcggtg gccggcaaca
    14701 acggggccgg ggctccggcg ggatatgagt caagtgaagt cgacggcctg cgcctgacct
    14761 ggcaggaagt cacctcggtc cagcgggccg atcaccgcct cgaccagctt cgcgccgtcg
    14821 cctgagggct ggccggcccg actcccgatc gagggtcata agctccgggt tgtcagcggt
    14881 cgaggagagg agccggagtg agtcggattt tcggaaaggt cgcccagaac gggtatgtcg
    14941 tcaaggacat caggtccgag atggatcact gggtcgggct cggcgtcggc ccctggtttt
    15001 acatgggccg ggtcgagatg gactggtttc gccatcgcgg cgaagattcg gaaccgcccg
    15061 aggtgagcat cgccctggcc aactcgggtg acctgcagat cgagctgatc cagcagcgaa
    15121 acgacgcccc ttctatgtac agggaattca tcgacgccgg ccacgagggt ctccagcaca
    15181 tgtcctactg gagcgaggat taccagtccc tctacgacaa ggtcctcggg ttgggcttcg
    15241 agatcgggca cgagggctgc atcggcggcg agcagggccg cttcgcctac ctggacacac
    15301 agacgacccc ggggaccgtg atcgagatct cggatgtgag cggagccaag gggatgttct
    15361 tcgagcatgt ccgctccgag gccgagaact gggatggctc ggacccgatc cgcgagctga
    15421 tccccgacc
//
LOCUS       DAOQKN010000039        15414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_1764_length_15414_cov_5.529592, whole genome shotgun sequence.
ACCESSION   DAOQKN010000039 DAOQKN010000000
VERSION     DAOQKN010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 15414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15414
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1764_length_15414_cov_5.529592"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="PLJ64_04230"
     CDS             <1..513
                     /locus_tag="PLJ64_04230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC92728.1"
                     /translation="LALVGPDSAGVDDDPEGAAVYEEVLGQFRALPAEVRERVHLVSL
                     PMEDLDENAFMVNAIQRRADVIVQKSLAEGFGLTVSEGMWKARPMVASRIGGIKDQIE
                     DGTSGILVDDPRDLPAFAAAIDSLLEDRERAAAIGDAAKLKVKSGLLSVQRLALHYQL
                     IDHLISPRPS"
     gene            complement(431..1618)
                     /locus_tag="PLJ64_04235"
     CDS             complement(431..1618)
                     /locus_tag="PLJ64_04235"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNC92729.1"
                     /translation="MIRGRIRGRAGHEERSSADQVFEIDPGELTNVFSVPTWLRNAGL
                     GAWFLVGIAILLLSLVWLASLTEVIVVPVIVASIVGAVGSPLVSKMNGFGLPRAIGAI
                     LVMLLLVLVGAACAVMVVNGIVGEVPEISRDLDKATDDLSQFLTDQGIDEEQVEKAKE
                     GAKEGATASIGTLLSGVGSGIAGISSAFFFLAMVLLSSFFMLKDGPTIRGWAERATSL
                     PPPVARIVSGRMISSLRGYFAGVTIVAAFSAVVVMIGALIIGVPLVATIGAVTFVAGY
                     IPYLGAWTAGIFTVLLAYGSGGTDAAMGMAVVQLLANGILQQMIQPFAMGSTLGIHPL
                     AVLVVTIAGGALFGSIGLIIAAPLVAAAAGIVTDLKTAEERSGGQSAGSGGPAAEPTA
                     APT"
     gene            1696..2586
                     /locus_tag="PLJ64_04240"
     CDS             1696..2586
                     /locus_tag="PLJ64_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC92730.1"
                     /translation="MPAIEIHDLKKSFGPINAVDGLDLVVDEGVCLGLLGPNGAGKST
                     TMKMLTGQAIPDSGVIRVLGYELPGQAKQARAAMGVVPQLDNLDIDVTVEENLAVFAR
                     LYRVDDVAAAVERTLEIARLTDRRKDAVDKLSGGMRRRLLLARGLVHEPKLLLLDEPT
                     VGLDPQIRTELWSLIDNLRSRGTTILMSTHYIEEAERLADEVAVVAGGKVIARATPKE
                     LIAEHAGEKTAEVYGPPERLTEVRSAAEEAGLRVRAAGPAVAIVGAEMAGPGLIPHDA
                     IHRAGSLEDVFVMLTGEDAE"
     gene            2583..3377
                     /locus_tag="PLJ64_04245"
     CDS             2583..3377
                     /locus_tag="PLJ64_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC92731.1"
                     /translation="MKPAGETRSVRRLEPAVILAVMGREFANFRTFWKSTAFTSTFEP
                     VVYLLAFGLGLGSTLVADVDGIDYVQFVGTGLVATAVVFSSALPAMFGTFVKERFQRT
                     YDAILAAPVDVEELVTAEMLWIGLRAAVFGCFPLIVTMFFGLEPRVTMLLVPIFAFVT
                     ALAFSAFGITMAALVSKIDQFNYVTTLVITPLFLVAGTFFPIDQLPQGFQIAAQFNPL
                     YQLVQLVRDACFGMEAVDALRFLGLLTLAFVLWRIAINRLTKRLIL"
     gene            complement(3411..4118)
                     /gene="rpiA"
                     /locus_tag="PLJ64_04250"
     CDS             complement(3411..4118)
                     /gene="rpiA"
                     /locus_tag="PLJ64_04250"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HNC92732.1"
                     /translation="MKPARHREVTFEPFEASSISNYEAKFKAAEEVAEMAQSGQVIGA
                     GSGSTAFLAVRAIASRVRHGELHDIRLIPTSIESELTITGLGLVQADLAEVAPDWLFD
                     GADEVDPDGNLIKGRGGALFREKLLFSACQLRRVLIDPSKRVDRLGTSFAVPVEVVPR
                     AASVATASLKSLGATEIVIRTGSGKDGPVLTEQGNLLVDCRFETIEDSLEDEINLITG
                     VLDSGLFQNYAPEIIEA"
     regulatory      4207..4352
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            4390..4947
                     /locus_tag="PLJ64_04255"
     CDS             4390..4947
                     /locus_tag="PLJ64_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92733.1"
                     /translation="MVRSVAIGLTGLLACFLIFALGTPAGAAASCKAASSSGGLSVGA
                     TKCKPFRKARLIRGKAYAPASAPARVKKVIAWANRIRNKPYIYGGGHGSFNDNGYDCS
                     GSVSYALRGGRFVAYPLASTGFMNWRSSGKGRWISVYANSGHAYMIVAGLRFDTSMTP
                     GNGPGWSKSLRSTPGSFTVRHPAGF"
     gene            complement(5007..5642)
                     /locus_tag="PLJ64_04260"
     CDS             complement(5007..5642)
                     /locus_tag="PLJ64_04260"
                     /inference="COORDINATES: protein motif:HMM:NF037625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC92734.1"
                     /translation="MFTVAANSLRERKKAKTRETIVSEALRLFAEDGYSQTTIARVAE
                     QADVSPRTVATYFPAKEDIVFYLSDRTKERLIRGIEDRPEGVDTMTALRQWVLEEREL
                     FEEHHELMGCQQRVIENEESLRAHEQALMRDFETVLAEGLAVDLGMEPTDLEPRLAAA
                     AAMAVFDLLGDDRKHRAEGELPPVEEQLAKLDQALTFIAGGVAALRDRQPG"
     gene            5737..7941
                     /locus_tag="PLJ64_04265"
     CDS             5737..7941
                     /locus_tag="PLJ64_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNC92735.1"
                     /translation="MSDLLARLARSITGHWKTALLIAIASVIGIGILSGTAGEAPPEN
                     FDVPGTESQQAYDLFTEHSPALAGVDATVVFNATEGSVDQPARKQAIEESLKEIKGQP
                     DVLTVSNPVSEKAGAVSKDGKVAYVDVRYDLDFGDVTKVQSERLEEATDPVKSEGVEV
                     AMRGIVIDAGQQEQAPVGELIGVIIAIFLLTLLFRSISAMIATLLGALIGVMVGQMLL
                     VILAAPLGLPDFAATIAMMLGLGAGIDYSLLILGRYREQAAAGDSTRDAAAKSAATSG
                     SAVLAAGLIVMVAIAGLLVIGIPLIGKMGIGAAIGVAAVVVSALTFLPIMIGALQRWL
                     KPKKLKHVLPSPAFGRWAERITGKPWISIAIGVVVLLVFASPLLDMRLGSPDDGNQPE
                     GKTQRVAYDLISDGFGKGVNGPFLIAVDTPDGAKQSKQQLDAVVKDLRQVDGVANVSP
                     AAPSEDNEMATIFLIPTTSPQDAATSDLLTELRDTVLPDATEGTGLSAYVGGNTASFE
                     DFSDKVSERLPVFIMVVIGLSVLLLMAAFRSLWVPLVSAVFNLLSIGAAYGVVVAVFQ
                     KGIGASLIGVDSGVPIMSFIPVMIFAILFGLSMDYNVFLLSRIHEAYNEGDRPRESVI
                     HGLSRIGKVILFAGLIMASVFSAFVTQDDVVAKMMGIGLGLAILIDVLIVRLVIAPAV
                     VTLLGDKAWWLPGWLDRILPNVSLEGHLVENRDPKGEPLAPELANDPIYEKP"
     gene            complement(7990..8601)
                     /locus_tag="PLJ64_04270"
     CDS             complement(7990..8601)
                     /locus_tag="PLJ64_04270"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC92736.1"
                     /translation="MSEDRRRQKERTAATRAAVLEATVELLVQRGYAGTSTRLAAEQA
                     GVSLGALQHHFPTKAELTVESMRFVTDRLADEFVSAIPRDGDELERISLALDRLLVVF
                     RGPTFAAALELHLAARTDESLREPIRRLHLEDQNRFRESAIALMPGVEDQPGFDAWLL
                     MSVSAIRGMAVATMGVAEVEDEWQLLKAQLLDSATELLRQESA"
     gene            complement(8781..11492)
                     /locus_tag="PLJ64_04275"
     CDS             complement(8781..11492)
                     /locus_tag="PLJ64_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92737.1"
                     /translation="MNIQSFVRRILPALIILVGGFTATGTQAIAAGKARPVKVSIAAI
                     STANQQQLIKQKKLAVKVKSTGKVSARLIATSGGEGGYFKATTVKFSRKGSKTVKLAL
                     TSSGKTALSKCGAKTVTTVASYRRKVGGKSKQAKSSKKKTLAKWAANENCIEYASVEL
                     GDDPEHCDFMDTTVCLQPWPNDYYMKDDASTPTGKRLDIAPGATPENTDGTHIAVTDI
                     NRGDGFSPGNLIVLKVPGLDTPAAFANNDFVTLSDLHAYDDPAQRVMLIDAATGERQP
                     IWTELDANPTSTDPGEDDPGGINTNPSNTTPVNLIVRPARNLEWGHRYIVAFRDLRDA
                     SDNPIQSPLGFRVYRDELPTRQAIVEARRDHMNSVIGDLVDKAGVERSSLYMAWDFTV
                     ASQGSVTSRALQVRDDAFARLGDNNLGNRVIEGSSPDVQIEYVCDRYENPDSENPVSD
                     GCNGGPNPPSGTDRRPDVPGSDIIRVIDGEILDVPCYLNQNGCPSGATFEFKPDGSID
                     FNENFTTDVPFRCFIPSSVQPAGQNTAVTPGQAGIYGHGLLGDRYQVTSAGPVNVATQ
                     GGSVWCGADWQGFSTGDLLSVFQALGDMSNFPKMADRMLQGFLNFMMIQRAMIHPDGF
                     ADLPAFQLDPDGPGGEDPVSVIDVSAGANTRGVYYGISQGGIMGGALMALTPDSDYGV
                     LGVPGMNYSNLLRRSVDSDTYFKTPGVGLYTHYPDEHERPLMLSLIQLLWDRGEANGY
                     ANNMTNDPLPDTPAHGVLLEVAYGDHQVTNYSAEVEARTIGAGIYSPALNAGRHWDVD
                     PFLGMDQISSFPHQGSMLVYYDSGPVSFTGNRGQGIGKPPLEEMPPRSEWGYGRDPHE
                     DPRRSPDGIDQATTFLQNGTIASCEVITPLPSGDAHCYANGYTGPTP"
     gene            11604..12080
                     /locus_tag="PLJ64_04280"
     CDS             11604..12080
                     /locus_tag="PLJ64_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92738.1"
                     /translation="MSRDSDTNYAGAIYGTIVSMAVIATSAKDPSLGPVLIAFWAAVT
                     ALIFWLAHVYADIVAEGYARFRDAGQVARHALRREWPLVQGAMIPSVAMLLTVVTPIN
                     TDTATWVAMGAGMVMLFATGLLIGARDQMSWSRRIFVGCVNASFGLVIVGLKVWVH"
     gene            complement(12156..12428)
                     /locus_tag="PLJ64_04285"
     CDS             complement(12156..12428)
                     /locus_tag="PLJ64_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92739.1"
                     /translation="MGCFVVLFALISPRLALFFILIFSDWIGHAIDGWIVPMLGFFFL
                     PWTLLAYVIMWQVSPHGVEGFDWFIVGFGFVVDIAAYARTSQSRQN"
     gene            complement(12438..13277)
                     /locus_tag="PLJ64_04290"
     CDS             complement(12438..13277)
                     /locus_tag="PLJ64_04290"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNC92740.1"
                     /translation="MPEPLRRFGAACWEVLLSFDRSAGTRQAAQLSFFVLLSFPALIL
                     LAVWILSNIFDSPDVRQDLIREIINNLPIEEIQGKREIEDMLDELTKGAGGLGIFSIV
                     ILLYSVSSAIGGLRHAVETANEKGDSGPPFPQNKLLDIGITLVTLPAALIFVALALSR
                     DLARVVDDNVFLSWSADHLGGPLGIFAAALIFYTWIFWVLNPGPTSWTSAAAGSLVTS
                     ALIWLVYSGLRIWFGISGGGSAIYGALAGFLGLLLFLNLASIAVVMGAHVAAIWRVHR
                     EAG"
     gene            complement(13321..13749)
                     /locus_tag="PLJ64_04295"
     CDS             complement(13321..13749)
                     /locus_tag="PLJ64_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92741.1"
                     /translation="MPETSGSQPPGGSAAWASWVAFAGIMLMLIGVFSVIAGLAAVTD
                     DGYIANASLHEVFLVSFKALGVFWILLGILKVLAGWALIQGREWARIVTVILVFLSAI
                     SHLLDLTSQPYLSILMIALDLVILYAVTVRWEAAKIGMGD"
     gene            complement(13815..14774)
                     /locus_tag="PLJ64_04300"
     CDS             complement(13815..14774)
                     /locus_tag="PLJ64_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNC92742.1"
                     /translation="MEAALDPAGPPPAEGMVWIPGGTFTMGSERHYPEEKPCGPVSVD
                     GFWMDETAVTNRQFAQFVSDTGYVTTAEKSPDPAMYPGADPAMLVPGSITFAKPSGPV
                     DLSNPMQWWAWTPGAQWRHPWGPASNLDGKEDHPVTQVTWEDAEAYCRWAGKELPTEV
                     QWERAGRGGIEGAEFAWGDEIAPGGEEKMNRWIGEFPWKFRPRPGGARKPGTVAVRTF
                     EPNPFGLYEITGNAWEWTKSWYADSHPEPVSRCCAPENPLGPGVEASRDPASGIPRKV
                     LKGGSFLCAENYCSRYRPSSRIPESIESSTVHITFRCVVPDAG"
     gene            complement(14798..>15414)
                     /locus_tag="PLJ64_04305"
     CDS             complement(14798..>15414)
                     /locus_tag="PLJ64_04305"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC92743.1"
                     /translation="YDYEGLGRDLDQVIEANPAENGMVLGGHSMGCHTVARRALMDPR
                     GVRALILIGPVFTGEADDDSRWDRRADALANGGPEAFAEIVAENSPNEEVREVTYRLA
                     RDRARLHRHPEAVAMALREVPRSLPFEGVNRLAALDLPVLVVASHDEFDPGHPRQVAE
                     LWAETIPGAELAGEEPGQSPLAWQGGLLSREIESFLGRHGLAAG"
BASE COUNT         2588 a         5100 c         4984 g         2742 t
ORIGIN      
        1 ctggccctgg tcgggccgga cagcgccggg gtcgacgacg acccggaggg cgccgccgtc
       61 tacgaagagg tgctcgggca gttccgcgcg ctgcccgcag aggtccgcga gcgggtccat
      121 ctggtcagcc tgccgatgga ggaccttgac gagaatgcct tcatggtcaa cgccatccag
      181 cgtcgggccg acgtgatcgt ccagaaaagc ctggccgaag ggtttgggct gaccgtctcg
      241 gaggggatgt ggaaggcccg gccgatggtc gccagccgga tcggcggcat taaggaccag
      301 atcgaggacg gcacctcggg catcctggtc gatgaccccc gggatctgcc tgcctttgcc
      361 gcggcaatcg attccctgct cgaagaccgg gaacgggcag ccgcaatcgg cgatgccgca
      421 aagctcaagg tcaagtcggg gctgctgtcg gttcagcggc tggccctcca ctaccagctg
      481 attgaccacc tgatctctcc tcggccgtct tgaggtcggt cacgatcccg gccgccgcgg
      541 caaccagcgg cgcggcgatg atcaggccga tcgatccgaa cagtgcgcca ccggcgatgg
      601 tgaccaccag cacggccagg ggatggatgc cgagggtgct gcccatcgcg aacggctgaa
      661 tcatctgctg gaggattccg ttggcgagca gctggacgac cgccatcccc atcgcggcat
      721 cggttccacc ggatccgtag gccagcagca cggtgaagat tccggccgtc caggcaccga
      781 ggtacgggat gtagccggcc acgaaggtga cggcgccgat cgtcgcgacc agcggcactc
      841 cgattatcag cgccccgatc atcaccacca cggcactgaa ggcggcaacg atcgtgaccc
      901 cggcgaaata tccccgcagc gacgaaatca tccggcccga aacgatccgt gccaccggag
      961 gcggcaggct cgtcgcccgc tccgcccagc ccctgatcgt cggaccgtcc ttcagcatga
     1021 agaacgagct gagcaggacc atcgcgagga agaagaaggc cgaagaaatc ccggcgatgc
     1081 ccgaaccgac cccggaaagc agggtcccga tgctggcagt tgctccttct ttggcaccct
     1141 ccttggcctt ctcgacctgt tcctcgtcga ttccctggtc ggtcaggaac tggctcaggt
     1201 cgtcggtggc cttgtccagg tcccggctga tctcagggac ctcgccgacg atcccgttga
     1261 ccaccatcac cgcgcaggcg gcaccgacca ggaccagcag cagcatgacc aggatcgcgc
     1321 cgatcgcccg cggcagaccg aacccgttca tcttcgaaac aaggggcgag ccgaccgcac
     1381 cgacgatcga ggcgacgatc accggcacca cgatcacctc ggtcagcgag gcaagccaga
     1441 ccagcgaaag cagcaggatc gcgatgccga ccagaaacca ggcgcccagc ccggcgttcc
     1501 tcagccaggt cggcacggaa aagacgttgg tcaactcgcc gggatcgatc tcgaagacct
     1561 ggtcggcgga agaccgctcc tcgtgccccg cccggcctct gattctgccc ctgatcatgg
     1621 ctcgcaggct accgccggaa ccagacggtt ccggggacca cggggccggg ttcgccggtc
     1681 tgattgactg ccccgatgcc cgcgatcgaa atccacgacc tgaagaagag cttcggcccg
     1741 atcaacgccg tcgatggact cgatctggtc gtcgatgaag gtgtctgcct gggccttctc
     1801 ggtccgaacg gcgccggcaa gtcgaccacg atgaagatgc ttaccgggca ggcaattccc
     1861 gattccggcg tgatccgggt actcggctac gaactgccgg ggcaggccaa gcaggcgcgt
     1921 gcggcgatgg gggtcgtgcc gcagctggac aatctcgaca tcgacgtgac ggtcgaggag
     1981 aaccttgccg tattcgcccg cctctaccgg gtcgacgatg tcgccgccgc ggtcgagcga
     2041 acgctcgaga tcgcccggct gaccgatcgt cgcaaggacg ccgtcgacaa gctctccggc
     2101 ggcatgcgcc gccggctcct gctggcaagg ggcctggtcc acgagccgaa actgctgctg
     2161 ctcgacgagc ccacggttgg cctcgacccc cagatccgga ccgagctctg gagcctgatc
     2221 gacaacctgc gctcacgcgg caccacgatc ctgatgtcca ctcactacat cgaagaagcc
     2281 gaaaggctgg ccgatgaggt cgcggtggtt gccggaggca aggtgatcgc ccgtgccacc
     2341 ccgaaggaac tgatcgccga acatgccggc gagaagaccg ccgaggtcta cggcccgccc
     2401 gagcggctga ccgaggtccg ctcggcagcc gaggaagccg gtctcagggt ccgggcggcc
     2461 ggtccggcgg tcgcgatcgt cggagccgag atggccggac cggggctgat cccgcacgac
     2521 gccatccacc gggccggctc gctcgaggac gtcttcgtga tgctgaccgg cgaggacgcc
     2581 gaatgaagcc ggccggcgag acccgctcgg tgcgccggct cgaaccggcc gtgatcctgg
     2641 ccgtgatggg ccgggaattc gccaacttcc ggactttctg gaagtcgacc gccttcacca
     2701 gcacctttga gccggtcgtc tacctgctgg cctttggcct tggcctcggg tcgaccctgg
     2761 tcgccgacgt cgacgggatc gactacgtcc agttcgtcgg caccggcctg gtcgcgaccg
     2821 cggtcgtctt ctcttcagcc ctgccggcca tgttcggcac cttcgtcaag gagcgcttcc
     2881 agcgaaccta tgacgcgatc ctcgcggcgc cggtcgacgt cgaggaactg gtcaccgcgg
     2941 agatgctctg gatcggcctg cgggcagcgg tctttggctg cttcccgctg atcgtgacga
     3001 tgttcttcgg gctcgagccc cgggtgacca tgctgctggt cccgattttt gccttcgtca
     3061 cggcgctcgc cttttccgcc ttcgggataa ccatggccgc cctggtttcg aagatcgacc
     3121 agttcaacta cgtcacgacc ctggtcatca cgccgctctt tctggttgcc ggcaccttct
     3181 tcccgatcga ccagttgccc cagggttttc agatcgcggc ccagttcaac ccgctctacc
     3241 agctcgttca gttggtgcgc gatgcctgct tcggcatgga ggcggtcgac gccctccgct
     3301 tcctcggcct gctgaccctc gccttcgtcc tctggcgaat cgcgatcaac cgcctcacca
     3361 aacgcctcat cctttgactg cagtcgggcc ggtcttcgcc ggccaggggc tcaggcttcg
     3421 attatttcgg gagcgtaatt ctggaagagg ccggagtcga ggacgccggt gatcaggttg
     3481 atctcatcct cgagactgtc ttcgatcgtt tcgaaccggc agtcgaccag gagattgccc
     3541 tgttcggtca ggaccgggcc gtccttgccg gatccggtcc ggatcacgat ctcggtggct
     3601 ccgagcgact tgagcgaggc ggtcgcgacg gatgcggccc gcggcacgac ttcgaccggc
     3661 accgcgaagc tggtgcccag ccggtccacc cgctttgacg ggtcgatcag cacccggcgc
     3721 agttgacatg ccgaaaagag cagcttttcc cgaaagagcg cgccgccgcg acccttgatc
     3781 agattgccgt ccggatcaac ctcgtcggcc ccgtcgaaca gccagtcggg cgccacctcg
     3841 gccagatcgg cctgaaccag gccaaggccg gtgatcgtca gttcggactc gatcgaggtc
     3901 gggatcagcc ggatgtcgtg aagttccccg tggcgcaccc gcgaggcgat cgcccgcacc
     3961 gccagaaagg cggtcgagcc ggagccggcc ccgatcacct gaccggactg ggccatttcg
     4021 gcaacttctt cggccgcctt gaatttggct tcgtaattgg aaatcgagga tgcttcgaag
     4081 ggttcgaacg tgacttctcg gtgtcgggca ggtttcacga tcaagagcct agggcataga
     4141 aacttctggc tttgcgctgg tttgttagca aagtattagt aaagtctgta cacttcacca
     4201 ttctgaaaaa ccgatttgcc gaattcccgt tgccgcaagg cccgggaagc gggggaccca
     4261 ggtagttggg gcgaacccac tcgaccgagt gagagcgcga ctcctaaagc gctagtccgt
     4321 cagctaactc cgcaggcact gaaaaaggag atcggcatca tcccctcaac ccctcatatt
     4381 cagagatcca tggttcgcag cgtcgcgatc gggcttaccg gtctgctcgc ctgcttcctc
     4441 atcttcgctc ttggcacgcc tgccggcgcc gcggcctcct gcaaggcggc ctcttccagc
     4501 ggcggtcttt cggtcggtgc gaccaagtgc aagccgttcc gcaaggcaag gctaatccgc
     4561 ggcaaggcgt acgctccggc cagcgccccg gccagggtca agaaggtaat cgcctgggcc
     4621 aaccggattc gcaacaagcc ctacatctac ggcggcggcc acggcagctt caatgacaac
     4681 ggttacgact gctccggttc ggtcagctac gcgctgcgtg gcggccggtt cgttgcctac
     4741 cccctcgcct cgaccggttt catgaactgg cgcagttcgg gcaagggccg ctggatctcg
     4801 gtctacgcga actccggcca tgcctacatg atcgtggccg ggctgcggtt tgacacctcg
     4861 atgaccccgg gcaacggtcc cggctggagc aagagcctgc gctccacccc cggctccttc
     4921 accgtgcggc atcccgccgg tttctaggac catccctccc ggaagtaacg accgccccgg
     4981 ctcccctcgc cggggcggtt ttgttttcag cccggttgcc ggtcgcgcag ggccgccacc
     5041 ccgccggcaa tgaaggtcag ggcctggtcg agctttgcca gctgctcctc caccggaggc
     5101 agctcgcctt cggcccggtg ctttcgatcg tcgcccagca ggtcgaagac ggccatggcc
     5161 gcggcggcgg cgaggcgcgg ttccaggtcg gtcggctcca tgcccaggtc aaccgcgaga
     5221 ccttcggcca gcaccgtctc gaagtcccgc atcaaagcct gttcgtgggc ccgcagcgat
     5281 tcctcgtttt cgatcacccg ctgctggcaa cccataagtt cgtgatgttc ttcaaagagc
     5341 tcacgctctt ccagcaccca ctggcgcaac gcggtcatcg tgtcgactcc ctccggacgg
     5401 tcttcgatgc cccggatcag ccgttccttg gtccggtcgc tgaggtagaa gacgatgtct
     5461 tccttggccg ggaaataggt cgcgacggtg cggggagaga cgtcggcctg ctcggcgacc
     5521 cgggcaatcg tggtctggct gtagccatcc tcggcaaaca gccggagcgc ctcggagacg
     5581 atcgtctccc tggtcttggc cttcttgcgc tcgcgcaggc tgtttgcggc gacggtgaac
     5641 accggccaag gttagcagcg attctgcata gactgcagtt ttgcagttgt tgtgataatt
     5701 tgcccgctct ctccctcccc tcacctctgg agctcaatgt ctgatctgct cgcccgcctt
     5761 gcccgttcga ttaccggtca ctggaagacc gccctcctca tcgccatcgc ttcggtgatc
     5821 gggatcggca tcctctccgg cacggccggc gaggcgccgc ccgagaactt cgacgtgccc
     5881 gggaccgaaa gccagcaggc atacgatctc ttcaccgagc acagcccggc cctggccggg
     5941 gtcgacgcca cggtcgtctt caatgcgacc gaaggcagcg ttgaccagcc ggcgagaaag
     6001 caggcgatcg aggaatcgct gaaggagatc aaggggcaac cggacgtgct cacggtcagc
     6061 aaccccgtct ccgagaaggc cggcgcggtc tcgaaggacg gcaaggtcgc ctatgtcgac
     6121 gtgcgatacg accttgactt cggcgatgtg accaaggtcc agtcggagcg cctcgaggaa
     6181 gccaccgacc cggtcaagag cgaaggcgtc gaggtcgcga tgcgcggcat cgtgatcgac
     6241 gccggccagc aggaacaggc cccggtcggc gagctgatcg gcgtgatcat cgcgatcttc
     6301 ctgcttaccc tgctcttccg ttcgatctcg gcgatgatcg cgaccctgct cggcgccctg
     6361 atcggggtca tggtcgggca gatgctgctg gtcatcctgg ccgccccgct cggattgccc
     6421 gattttgccg cgaccatcgc aatgatgctc ggcctcggcg ccggcattga ctattcgctc
     6481 ttgatcctcg gccgctaccg cgaacaggcg gcggctgggg attcaacccg ggacgccgcg
     6541 gcaaaatcgg ctgccacctc cggctcggcg gtgctcgccg ccggtttgat cgtgatggtc
     6601 gcgatcgccg gactgctggt gatcggaatc ccgctgatcg gcaagatggg aatcggcgcc
     6661 gcgatcgggg tcgccgcagt ggtggtctcg gccctgacct tcctgccgat catgatcggc
     6721 gcgctccagc gctggctcaa gccgaagaag ctcaagcatg tgctgccctc gcccgccttc
     6781 ggccgctggg ccgagcggat caccggcaag ccgtggatct cgatcgcgat cggggtcgtc
     6841 gtgctgctcg tattcgcctc tcccctgctc gacatgcgcc tcggttcgcc ggatgacggc
     6901 aaccagccgg agggcaagac ccagcgcgtc gcctacgacc tgatcagcga tggcttcggc
     6961 aagggcgtaa acggaccctt cctgatcgcc gtcgacaccc cggacggggc caaacagagc
     7021 aagcagcagc tcgacgccgt ggtcaaggac ctgcgccagg tcgacggggt cgccaacgtc
     7081 tcccccgccg cgcccagtga agacaacgag atggcgacga tcttcctgat cccgaccact
     7141 tcgccccagg acgccgcgac cagcgacctg ctcaccgagc ttcgcgacac cgtgctgccc
     7201 gatgcgaccg aaggcaccgg cctgagcgcc tacgtcggcg gcaacacggc cagcttcgag
     7261 gacttctcgg acaaggtctc ggagcgcctg ccggtcttca tcatggtcgt gatcgggctc
     7321 tcggtgctcc tgctgatggc cgccttccgc tcgctgtggg tgccgctcgt ttcagccgtc
     7381 ttcaacctgc tttcgatcgg ggccgcctac ggcgtggtgg ttgccgtctt ccagaagggg
     7441 atcggcgcca gcctgatcgg cgtggacagc ggcgtgccga tcatgtcctt catcccggtg
     7501 atgatcttcg cgatcctctt cgggctctcg atggactaca acgtcttcct tctgtcccgc
     7561 atccacgagg cctacaacga gggtgaccgc cctcgcgaaa gcgtgatcca cggcctctcg
     7621 cggatcggca aggtgatcct cttcgccggc ctgatcatgg cctcggtctt ctccgccttc
     7681 gtgacccagg atgacgtggt cgccaagatg atgggcatcg ggctcggtct ggcgatcctg
     7741 atcgacgtcc tgatcgtccg cctggtgatc gcaccggccg tggtcaccct gctcggtgac
     7801 aaggcctggt ggctgcccgg ctggctggac cggatcctgc caaacgtcag cctcgagggc
     7861 cacctggtcg agaaccgtga cccgaagggc gagccgctcg ctccggagct ggccaacgac
     7921 ccgatctacg agaagccctg acccggcagt tgccaaggcg ggcatggagg caccggcttc
     7981 ccggccgggt caggcggatt cctgccgcag cagttcggtt gccgagtcga gcagctgcgc
     8041 tttgagcagc tgccattcgt cttcgacctc ggcgacgccc atggtcgcga ccgccatgcc
     8101 gcgaatcgcc gaaaccgaca tcaggagcca ggcatcgaaa ccgggctgat cctcgactcc
     8161 gggcatgaga gcgatcgcac tctcccggaa gcggttctga tcctcgagat gaagccgccg
     8221 gatcggctcc cgcagggact cgtccgtgcg ggccgccagg tgaagttcca gcgcggcggc
     8281 gaaggtcggg ccccggaaga ccacgaggag acggtcgaga gcgagcgaaa tgcgttcgag
     8341 ctcgtcaccg tcacggggta tggccgagac gaactcatcg gccagccggt cggtgacgaa
     8401 tcgcatcgac tccaccgtga gttcggcctt ggtcgggaag tggtgctgca gcgcacccag
     8461 tgacaccccg gcctgctcgg ccgccagcct cgtggaagtt cccgcgtagc cacgctggac
     8521 cagcagctcg accgtggcct ccagcaccgc ggccctggtt gccgccgtcc tctccttctg
     8581 ccggcgcctg tcctcgctca cgccgtcctc gccggtctca tgagcgcaat atacatctgt
     8641 cggtcaaccg acttgtaagt tcagatcccc gcgtctcccc cgcttccggg ggcgtttgac
     8701 caccgaaatg gtggcctaac gcccccggaa gggcgggttc ggcgacggcc ggggcccggc
     8761 ggccggcccg gcccctccga tcagggggtc gggccggtgt agccgttcgc gtagcagtgg
     8821 gcgtcgccgg acggcagcgg ggtgatcacc tcgcagctgg cgatggttcc gttctggagg
     8881 aaggtggtcg cctgatcgat gccgtcgggc gagcgccgcg gatcctcatg tggatcgcgt
     8941 ccgtaacccc actccgagcg tggtggcatc tcctccagcg gcggcttgcc gatgccctgg
     9001 ccccggttgc cggtgaaact gaccggcccg ctgtcgtagt agacgagcat gctgccctgg
     9061 tgggggaagc tgctgatctg gtccattccg aggaagggat ccacatccca gtgccggccg
     9121 gcgttcaagg ccggcgaata gattccggcg ccgatcgtgc gagcctcgac ctccgccgag
     9181 tagttggtca cctgatggtc accgtaggcg acttccagca ggacgccgtg ggccggggtg
     9241 tccggcagcg ggtcgttggt catgttgttg gcatagccgt tggcctcgcc tctgtcccag
     9301 agcagctgga tcagggagag catgagcggc cgttcgtgtt cgtcggggta gtgcgtgtaa
     9361 aggccgaccc cgggcgtctt gaagtaggtg tcggaatcga ccgaccggcg cagcaggttt
     9421 gagtagttca ttcccggcac gccgagcacc ccgtagtcgg aatccggggt aagggccatc
     9481 agggcgccgc ccatgatgcc gccctggctg atcccgtaat agacgccgcg ggtgttcgct
     9541 ccggcggaca cgtcgatcac cgaaaccgga tcctctcctc cgggtccgtc cgggtcgagc
     9601 tggaaggcgg gcaggtccgc gaagccgtcc gggtggatca tcgcgcgctg gatcatcatg
     9661 aaattgagga atccctggag catccggtcg gccatcttcg ggaagttgga catgtcgccg
     9721 agggcctgaa acacggaaag gagatccccg gtcgagaagc cctgccagtc ggcgccacac
     9781 cagacgcttc cgccctgggt ggcgacgttg accgggccgg ccgaggtcac ctggtagcgg
     9841 tcaccgagca gcccgtggcc gtagattccg gcctggcccg gggtcacggc cgtattctgt
     9901 ccggccggct gcaccgaact gggtatgaag cagcggaacg gcacgtcggt cgtgaagttc
     9961 tcgttgaagt cgatcgaccc gtccggcttg aactcgaagg tggccccgga agggcagccg
    10021 ttctggttca ggtaacaggg aacgtcgagg atctctccgt caatcacgcg gatgatgtcg
    10081 gagccgggaa cgtcggggcg ccggtcggtt cccgatggcg ggttcgggcc accgttgcag
    10141 ccgtccgaaa ccgggttctc cgaatcgggg ttctcgtagc ggtcacagac gtactcgatc
    10201 tggacgtcgg ggcttgagcc ctcgatcacc cggttgccga ggttgttgtc acccagccgg
    10261 gcgaaggcgt cgtcgcggac ctgcagggcc ctcgaggtga cgctcccctg agaggcgacg
    10321 gtgaagtccc acgccatgta aaggctcgag cgctcgaccc cggccttgtc gaccagatcg
    10381 ccgatcaccg agttcatgtg gtcgcgccgc gcttcgacga tcgcctgccg ggtgggcagc
    10441 tcgtcacggt agacgcggaa tccgagcggc gactggatcg ggttgtcgct ggcgtccctg
    10501 aggtcgcgga aggcgacgat gtaacggtgg ccccactcga ggttccgggc cggtcggacg
    10561 atcaggttga ccggtgtggt gttggaagga ttcgtgttga ttccgcccgg gtcgtcctcg
    10621 cccggatcgg tcgaggtcgg gtttgcgtcg agttcggtcc agatcggctg gcgctcgccg
    10681 gtggcggcat cgatcagcat gacccgctgg gcggggtcgt cgtaggcatg aaggtcactc
    10741 agagtgacga aatcgttgtt ggcgaaggca gcgggggtgt ccagtccggg caccttcagc
    10801 acgatcaggt tgcccgggct gaagccgtca ccgcgattga tgtcggtgac cgcaatatgg
    10861 gtcccgtcgg tgttctcggg agtcgctccg ggagcgatgt ccagccgctt gccggtggga
    10921 gtcgaggcgt catccttcat gtagtagtcg ttcggccagg gctgcaggca gacggttgtg
    10981 tccatgaagt cgcagtgctc cggatcgtca ccgagctcga cgcttgcgta ttcgatgcag
    11041 ttctcgttgg ccgcccactt ggcgagtgtc ttcttcttcg aggacttcgc ctgcttcgac
    11101 tttccgccga ccttgcggcg gtagctggcg accgtcgtca cggtcttcgc tccacatttg
    11161 gaaagcgcgg tctttccgga tgaggtgagg gcgagtttca cggtcttgct gcccttgcgg
    11221 gagaacttca ccgtggtggc cttgaagtag ccgccctcgc caccactcgt cgcgatcagc
    11281 cgggcagaga ccttgcccgt cgatttgacc ttcacggcca gcttcttctg cttgatcagc
    11341 tgctgctgat tggcagtgct gattgccgcg atgctcacct tgaccggtct ggccttgccg
    11401 gcagcaatgg cctgggtgcc ggtggcagtg aacccgccga cgaggatgat cagcgccgga
    11461 agaatccggc ggacgaaact ctgaatgttc aaaaggtctc cctgattgct ttttcatccg
    11521 cggattcctt ccgccggaac acccactctc caagggcggg ggcattctag cccacggcgc
    11581 ccgggtcact atggttctgc gctgtgagcc gcgattcgga caccaactac gcgggggcga
    11641 tctacggcac gatcgtctcg atggcggtta ttgcgacctc ggccaaggac ccttcgctgg
    11701 gtccggtgct gatcgccttc tgggccgccg tcaccgccct gatcttctgg ctggcccatg
    11761 tctacgccga catcgtggcc gagggttacg cccggttccg tgatgccggc caggtggcca
    11821 ggcacgccct gcgacgggaa tggcccctgg ttcagggagc gatgatccct tcggtcgcga
    11881 tgctgctcac cgtggtgacc ccgatcaaca ccgatacggc aacctgggtc gcgatgggag
    11941 cgggcatggt gatgctgttc gccaccggtc tcctgatcgg cgcccgggat cagatgtcat
    12001 ggagccgccg gatcttcgtc ggttgcgtca acgccagttt cggactggta atcgtcggcc
    12061 tgaaggtctg ggtccactga tccggggccg ggtccggcca tcggccggca cccgcctggc
    12121 gtgatgcctt ccggcagtga aatgccggga tcggctcagt tctgcctgga ctgggaggtg
    12181 cgggcgtagg cggcgatgtc aaccacgaaa ccaaagccga ctatgaacca gtcgaagccc
    12241 tccaccccat gcgggctgac ctgccacatg atcacgtagg cgagcagggt ccacggcagg
    12301 aagaagaaac ccaacatcgg cacgatccag ccgtcgatcg catgcccgat ccagtcagag
    12361 aagatcagga tgaagaagag cgccagtcgc ggcgagatca gtgcgaagag aactacgaag
    12421 caacccatgc ctgcaatcta cccggcctca cggtggaccc gccagatcgc cgccacgtga
    12481 gcccccatca cgaccgcgat cgaggccagg ttgaggaaga gcagcaggcc gaggaacccg
    12541 gccagcgccc cgtagatcgc gctgccgccg cccgagattc cgaaccagat ccgcaggccc
    12601 gagtacacga gccagatcag ggccgaagta accagtgaac ccgccgccgc gctcgtccag
    12661 ctcgtcgggc ccgggttcag cacccagaag atccaggtgt agaagatcag ggcagcggcg
    12721 aatatgccca acggtccgcc gaggtggtcg gcgctccacg aaaggaagac gttgtcatcg
    12781 acgaccctgg cgaggtcgcg ggaaagggcc agcgcgacga agatcagcgc cgccggaagg
    12841 gtgaccaggg tgatgccgat gtcaagcagc ttgttctggg ggaagggcgg gcccgaatcg
    12901 cccttctcgt tggcggtctc gaccgcgtga cgaaggccgc caatcgccga gctgaccgag
    12961 tagagcagga tcactatgga gaagattccg agcccgccgg cccccttggt cagctcatcg
    13021 agcatgtcct cgatttctct tttgccctgg atctcctcga tcgggaggtt gttgatgatc
    13081 tcccggatca ggtcctggcg cacgtccggc gagtcgaaga tgttgctgag gatccagacc
    13141 gcgagcagga tcagggccgg gaagctcagc agcacgaaga acgacagctg ggccgcctgc
    13201 cgggtgcctg ccgaacggtc gaatgacagc agcacttccc agcaggcggc cccgaaccgc
    13261 cgcagcggct cgggcacctt cgaagcccag gccttcagtt ttgaagcccg ggtgggcgaa
    13321 tcagtccccc atgccgatct tcgccgcttc ccagcggacg gtcacggcgt aaaggatgac
    13381 caggtcaaga gcgatcatca ggatgctcag gtagggctgc gaggtcaggt cgagcaggtg
    13441 gctgatcgcc gagaggaaga ccaggatcac ggtcacgatc cgggcccact cgcggccctg
    13501 gatcaatgcc cagccggcaa ggaccttgag gattccgagc aggatccaaa acaccccgag
    13561 cgccttgaag ctgaccagga aaacctcatg gagggaagcg ttggcgatgt atccgtcatc
    13621 ggtcacggct gccaggccgg cgatgacgct gaacacgccg atcagcatca gcatgatgcc
    13681 ggcgaatgcg acccaggaag cccaggcggc ggatcctccc gggggctgtg aaccactggt
    13741 ttcaggcatt gaactcctcc atcggtttca tgtttggcag gccgccgggg gacgaacggg
    13801 cggctccatg aaccctatcc ggcgtcgggg acaacgcagc ggaaggtgat gtgcacggtt
    13861 gagctctcga tgctttccgg gattcgcgag gacggccggt atcgcgagca gtagttctcg
    13921 gcgcaaagga acgacccgcc tttcagcacc ttgcggggaa tgccgctggc cggatcacgg
    13981 ctcgcttcga ctcccgggcc gagcgggttc tcgggggcgc aacagcggct caccggttcc
    14041 ggatgcgaat ccgcgtacca gctcttcgtc cactcccagg cgtttccggt gatctcgtaa
    14101 agcccgaagg ggtttggctc gaaggtcctg accgccaccg tcccgggttt gcgggctccg
    14161 cctggccggg gccggaactt ccacgggaac tcgccgatcc agcggttcat cttctcttca
    14221 ccgccagggg cgatctcatc accccaggca aactcggccc cttcgattcc gccacggccg
    14281 gcccgttccc actgaacttc ggttggaagt tccttgccgg cccagcggca gtaggcctcg
    14341 gcgtcctccc aggtgacctg ggtgaccggg tggtcctcct tgccgtccag attgctggcg
    14401 ggaccccacg ggtgccgcca ctgggcgccg ggagtccagg cccaccactg catcgggttc
    14461 gagaggtcga ccgggccgga aggcttggcg aaggtaatcg agccaggcac cagcatcgcc
    14521 ggatcggcgc ccggatacat ggccgggtcc ggtgactttt ccgccgtcgt aacgtagccg
    14581 gtgtcggaaa cgaactgcgc aaactggcgg ttggtcaccg cggtctcatc catccagaaa
    14641 ccgtccaccg aaaccggccc gcagggcttc tcctccggat agtgcctttc gctgcccatc
    14701 gtgaaggttc ccccgggtat ccagaccatg ccttcggccg gcggcggccc ggctggatca
    14761 agtgcggctt ccatggagaa aaggctaccc gccgcgctca gccggcggcc agcccgtggc
    14821 ggccgagaaa gctttcgatt tcccgcgaaa gcaggcctcc ctgccaggcc agtggtgact
    14881 gacctggttc ctcgccggca agctcggccc cggggatcgt ttccgcccac agttccgcaa
    14941 cctggcgggg atggccggga tcgaactcgt cgtggcttgc gaccaccagc accggcagat
    15001 caagggcggc aagccgattg acaccttcga agggaagcga gcgcggcacc tcccgcaggg
    15061 ccatggcgac cgcttccggg tgacggtgga gcctggcccg gtcgcgggcg agacggtagg
    15121 tcacttcgcg aacttcctcg ttcgggctgt tctcggccac gatctccgca aatgcttccg
    15181 gcccgccgtt cgcaagggcg tcggcgcgac ggtcccatcg cgagtcgtcg tcggcctcgc
    15241 cggtgaacac cggtccgatc aggatcagcg cccgcacccc ccgggggtcc atgagggccc
    15301 gtctggccac ggtgtggcag cccatcgaat ggcctccgag gaccatcccg ttctccgccg
    15361 ggttcgcctc gatcacctga tcgaggtctc tgcccaggcc ctcgtagtcg tagc
//
LOCUS       DAOQKN010000040        15406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_1765_length_15406_cov_5.360889, whole genome shotgun sequence.
ACCESSION   DAOQKN010000040 DAOQKN010000000
VERSION     DAOQKN010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 15406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15406
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1765_length_15406_cov_5.360889"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1173)
                     /locus_tag="PLJ64_04310"
     CDS             complement(<1..1173)
                     /locus_tag="PLJ64_04310"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNC92744.1"
                     /translation="MNGTIVLNRFEIGDRLGAGGFGTVYRAWDRRLEREVAVKVIETA
                     AESGPRIQREAKAAARLNHTGIVTLYEFAHHEYGREGGRAYLVSELVDGHTVRKLMDR
                     DLISDREIGEIGIDICEALDHAHSREVIHRDIKPANLIVPNGRGGAKLMDFGVARLTD
                     GDDLTNTGDILGTLAYMSPEAAEGNPVTPAGDVYSLALTLFEAWTGENPRRRPTPTLA
                     LRAIERDLPLLADLRPDLPAEMTEVVDACLDREPGFRPGLEELGHALEDALPELDRSV
                     PDGNSRGAGLTSFADGGLDLGRIAAAAAVCGMVATGMIVSGAADAASVALLTVVSALL
                     TLLRPKAGFLAGGVLLAAWLSVIAAMPGAALVVLLVSVPPALLIRGSGRPLALAVVG"
     gene            1351..2592
                     /locus_tag="PLJ64_04315"
     CDS             1351..2592
                     /locus_tag="PLJ64_04315"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HNC92745.1"
                     /translation="MEFSNPYNPDDRENDPFSEGASDNPFEDDRTIVSEPDSDPDLEL
                     DDPGIPPPPPRRRSGGGSGRGGRPGGRGPRGGGSGGDRQQLMVRRAIALGAGLLVLIL
                     LVIGAKGCLDARKNRSLDDYANSVTQIVNETNALGKSFFGRLSDPGELSVTEFTSEIQ
                     SDRSAMDGFLSRVEKLDTPGDMSAAQDSLTLVYQLRASAMDDIADQMSTALGDEGAER
                     ARRQIAAQMEVLSAADVLYNRVTRHQIDNTIANNGASAPAMPVSTFIEDPQDWITVDA
                     INGALDDVSGSTSTTAPDDGLTHGTGIDPASSVTVDGTTLSPDTTTTIAASTDPTVEV
                     TVMNQGGATETDVTVGVSVDGGTSLEAKIPEIAAGSTGTATIPLTPAPTGEVTLDVSV
                     DAVPGEQFTTNNEASYTVSFG"
     gene            2600..3448
                     /gene="pheA"
                     /locus_tag="PLJ64_04320"
     CDS             2600..3448
                     /gene="pheA"
                     /locus_tag="PLJ64_04320"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HNC92746.1"
                     /translation="MRVAYLGPAGTFSEDALEVSRFAPEGFEAVVCETIPAAIEAVAA
                     GDADRALVPVENSIEGSVRPTLDGLIEHADAVRIAGEFDHEIRSALIARTALDPGSIE
                     VVISHPQPLAQCALFLRTELGQAEQRVATSTSAAVREVAESTGPWAALAPARAARIYE
                     CEVIREGIEDEPGNVTRFLWLAPESAENPVVGDQGGQWKTSLAFAELGADHPGALVEA
                     LHEFSDRRVNLVRIESRPMRRELGRYRFFIDIEGREDADDVTAAIAGLRSRAEWVRVL
                     GSYPAN"
     gene            complement(3479..5992)
                     /gene="gyrA"
                     /locus_tag="PLJ64_04325"
     CDS             complement(3479..5992)
                     /gene="gyrA"
                     /locus_tag="PLJ64_04325"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNC92747.1"
                     /translation="MALEALTGHIEERELELEMRSSYIDYAMSVIVGRALPDVRDGLK
                     PVHRRVLYGMHDLGLQPNRPYRKSATIVGEVMGKYHPHGDSAIYDTLVRMAQDFSLRY
                     PLVDGQGNFGSIDDDAAAAMRYTEARLTRLATEMLRDIDSDTVDFGPNYDGSQREPLV
                     LPSRFPNLLVNGSSGIAVGMATNIPPHNLTEVAGAVAAYIDNREIDLEGLMKHIKGPD
                     FPGGGLMDPGGIRDAYQTGRGSVKVRARIHSEPLKGGKDALIVSELPFTVKKGGDSGV
                     IAKIAQLVRDKKLDGISDLRDETDRTGMRLVIELKRGAVPNTVLANLFKKTQLQTSFG
                     MNMVSLVDGVPRTLGIRDMIVHYVEHQREVVTRRTQYKLRRAEARAHILEGLLIALDN
                     LDAVIALIRGSSDPDVARDGLISEFDLSREQAQAILDMRLQRLTALESDKIREEHAEL
                     MELIGELRAILGDEDRVYGLVRDELLELVETHGDERRTEFQHFGGDIDIEDTIAEQQM
                     VISLTNTGYVKRLPVDSYRQQRRGGVGVTGMNLKEGDYIEHLHICSTHDFLLFFTNFG
                     KVYRKKVYELPEGSRQARGSALVNLLPLREGERVMAVIPTREFKENKYLVFATAQGQV
                     KKTEFLDYNTPIKADGIIAIKIREGDELVGVQLTSGEDDLLMVSKSGHASRFHEDQAR
                     PMGRGTAGVRGMNVSQKDNRVLSLTVARDDSDLLVVTENGYGKRTGISEYPVKNRGTK
                     GVLTIKLTEAKGGLAGALIVREHQELMFITENGMVQRTGAGGISKTGRATQGVKVMNV
                     KDGDRISAVALVVEAANGENGDPEGEEAPEQPELEATEE"
     gene            complement(6029..8440)
                     /gene="gyrB"
                     /locus_tag="PLJ64_04330"
     CDS             complement(6029..8440)
                     /gene="gyrB"
                     /locus_tag="PLJ64_04330"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HNC92748.1"
                     /translation="MAEKKSSGDASYGAKDITVLEGLEAVRKRPGMYIGSTGPRGLHH
                     LVYEIVDNSVDEALAGHNESVEVNLHPDNSCTVTDHGRGIPVDIMEKEGRPAAEVVLT
                     VLHAGGKFGDGGGYKVSGGLHGVGSSVVNALSSWLELRICRDGFVWNQRYERGRPVTE
                     LEKGEATKETGTQITFLPDDEIFEELDFDFQTLLERLRETAFLTRGLRIQITDERAEG
                     QEVEFKYDGGIEDFVRYLNENKEPLGKKVVYFEGEDEKEGQVEVAMQWNASYNDSIHS
                     FANNINTHEGGSHLSGFQAALTRTLNAYARSKGLLKEKDANLAGEDVREGLAAVISVK
                     LHDPQFEGQTKTKLGNPGIKGMVQETVNRKLAEFLEENPAEARLFITKAIEASRARMA
                     ARKARDMTRRGSALENMNLPGKLADCSVRDPELAEIFVVEGDSAGGSAKQARDRSTQA
                     VLPLRGKIINVEKSRIDKVLQNNEIQAMITAIGTGIHDEFDITNARYHKIIMATDADV
                     DGAHIRTLVLTFLYRQMQPLIDAGYVFIAKPPLYKVKRGNQETYLDKESELEELLLRD
                     RLEDFLVTDSTGKETKLNSRRWQTYTRRSKEYEGWHAALQAEFGNDVISFIAGSRLLD
                     EAATTVDEVRRILGEVDREEDIFATEILSQDGEDTGIKAIHQRSGLARNHTLPARLFE
                     GDAFRRFAAVHEELLGTFGQPPFRVTLGERAEEASDFLDLHRAVLDLAKHGVQLTRFK
                     GLGEMNADQLRETTMDPATRTLQRVTLEDATAADQVFSMLMGDQVAPRREFIEAHAKE
                     AGIDI"
     gene            complement(8635..8943)
                     /locus_tag="PLJ64_04335"
     CDS             complement(8635..8943)
                     /locus_tag="PLJ64_04335"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HNC92749.1"
                     /translation="MRDARGRSPSPKRLGEVLGDFRGEVTPPTLLAAVQTQWDEVVGP
                     RIAGVTDVIDEREGVVTVECSSAVWAQELELMAPRILARLKDRIDGPVPEKLRFRAAR
                     "
     gene            complement(8940..10073)
                     /gene="recF"
                     /locus_tag="PLJ64_04340"
     CDS             complement(8940..10073)
                     /gene="recF"
                     /locus_tag="PLJ64_04340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HNC92750.1"
                     /translation="MIVSRVEADQVRSLAGVIFEPDPGLTVIIGPNGAGKTNLVEAIY
                     FGLTSRSFRTSDRRDLIPFGSAFARCRVTVQGGGVDHVFMASASRSEGNRFTMDGARI
                     EQAEAVLHRPKVTVFSPDRLELVKGPPAGRRAHLDSFVAARWPSRAGLRSTFGRILAQ
                     RNALIARISSAEANSSQLATWNSQFAEAGANLASGREAAVEALAERWVEAADDLGLEG
                     PKGISYRPGSATTKEEIEAGLEERLEHDLRLGRSSWGPHHDEVRLELDGRQLRRFGSQ
                     GQQRLGLLALLFAERAALLDAGEAAPLMLLDDVMSELDAHRRGRLVERLLGGGQSVIT
                     AAEGELIPERQEISRVAIRDLVGADEERDNGEDPGAGSGGGTS"
     gene            complement(10078..11190)
                     /gene="dnaN"
                     /locus_tag="PLJ64_04345"
     CDS             complement(10078..11190)
                     /gene="dnaN"
                     /locus_tag="PLJ64_04345"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNC92751.1"
                     /translation="MKFSIKNNELSGKLALVARTLATGGVNPALGGILVEATDGRLSF
                     SSTDSNLSLRVPVHVEGSVESDGTVLLPGRLFADISRLLGSGQSEIEYLASERKVSVK
                     SGSSSFNLRTLNEKDFPPLPDASGETISLGRESLLETIELVARAASRDDMRPVLTSVQ
                     VLASGNELTMVATDSYRLAVKQTHLAEAVSVELDRNIPANALRELSRLLSASESDSVR
                     LSVTDRLVVFNVDGSILSTNTVDGQFPKYQQLFPETWDHDVRLPRTELLESVRRVSQM
                     AQKNRPLKIALTPGELTISAETPDLGDAEEKMPANFDGEELAIGFNHEFFRDGLEAIP
                     GDEVLLRLISPLRPGLLQPVDSEDFRYLVMPIRLNE"
     gene            complement(11335..12756)
                     /gene="dnaA"
                     /locus_tag="PLJ64_04350"
     CDS             complement(11335..12756)
                     /gene="dnaA"
                     /locus_tag="PLJ64_04350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNC92752.1"
                     /translation="MSSERSPASLDQPFSDMRAATSSNRESKAGSRKSLPGSARAVKE
                     WETIQPTLKRRLFDSAWRMWIEPVTPVAVRGTTLYLQAPDPIRTWVERRYIEVITETL
                     VDSSSHLTAVSFENPPEPERVPAPTRPAGPNPTHDFDRFVIGPGNHLAHAAALAVAEA
                     PSESYNPLFLHGPPGLGKTHLLGAIANYLALHVPELNVLYTNAESFTAEFVTALRDQG
                     TDEFKRRYRNLDVLLIDDIQFIAGKPHTEEEFFHTFNALHDAGSQIVLSADRSPGEIS
                     TLADRLSDRFEWGLTVPIEAPNLATRLTVLRHLVTEAGLEVADQDALTLLARRVQSNL
                     RQLRGALTRTIAESSLTSAPITSELITQVFPATESAPAIQITPETIRSTVAAHYRTDA
                     DTLQSRRRDRNTATARHVAIYLTREITSLSLPQIGGLYGDRDHTTVLNSIERVEQSVG
                     QDAEITNAVEILRAQIHSPGENS"
     gene            12926..13060
                     /gene="rpmH"
                     /locus_tag="PLJ64_04355"
     CDS             12926..13060
                     /gene="rpmH"
                     /locus_tag="PLJ64_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012082253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNC92753.1"
                     /translation="MKRTYQPKKRKRAKTHGFRARMSSKGGREVIRRRRRKGRKRLAV
                     "
     gene            13063..13449
                     /gene="rnpA"
                     /locus_tag="PLJ64_04360"
     CDS             13063..13449
                     /gene="rnpA"
                     /locus_tag="PLJ64_04360"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNC92754.1"
                     /translation="MSANGAGSPRRRRLSRSGDFKRAYREGSSKATRYLVLYRFDRSA
                     DDESEIRLGVSVSKKLGDAVTRNRVKRVLKEAFWSQVDRDEADQDFVLVARPGVGEVI
                     DTDGLAGAEACVKEVLELGKGGKRST"
     gene            13446..13721
                     /gene="yidD"
                     /locus_tag="PLJ64_04365"
     CDS             13446..13721
                     /gene="yidD"
                     /locus_tag="PLJ64_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNC92755.1"
                     /translation="MKRIILSPVRAYQRWISPLLPRRCKYEPTCSVYAVEAVERFGVI
                     RGTVLACWRILRCNPFSHGGFDPVRDEFTLKVGPMDPAVYHGETEQR"
     gene            13721..14536
                     /locus_tag="PLJ64_04370"
     CDS             13721..14536
                     /locus_tag="PLJ64_04370"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HNC92756.1"
                     /translation="MPIANILQPLIDVANAVLSFFHDDLGASWGMAIILLTICTRAVL
                     IPLTYRQLKSMRALQAFQPQMKELQEKYKNDRQRLSQEMMKFYQENKINPLASCIPLL
                     AQLPVFITLFQALRGPLREDICGQTAVACSQLTQAQIDANPAIHALYPFGESFLFIPD
                     LTDKATGGVLIALLVLYVGTQLISGVISMTTGDRNQRMLMLALPFIFVPFIISFPAGL
                     ILYWITTNVWTIGQTYVINRVIPPPVMSTPEGVVVAAAAKPPPPPPKKKKRKR"
     gene            14568..15047
                     /locus_tag="PLJ64_04375"
     CDS             14568..15047
                     /locus_tag="PLJ64_04375"
                     /inference="COORDINATES: protein motif:HMM:NF024483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HNC92757.1"
                     /translation="MEASEWPAEPAERVRLLVERVLDELDLDGEVEMDETDDVITATV
                     EGDEDYGLLIGRRGQTIDALQLLAFQAAFRGIRERKRVVVDAAGYRARREEVISERAD
                     RAAEKAIASGSEVELDPMSARERRIVHEHLKSRPEVETFSSGDDPHRRVVIAPLITE"
     gene            15058..>15406
                     /locus_tag="PLJ64_04380"
     CDS             15058..>15406
                     /locus_tag="PLJ64_04380"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC92758.1"
                     /translation="MNSVTPPPPPAGPSAGEGTTGGEAEPWLDREALARLDPMLDLLA
                     ESHVSLSSVTDPAEARRVHVADSLSGLACPEVRAAKRCADLGAGAGFPGIPLAVFLPE
                     CEFVLIDSVGRKVD"
BASE COUNT         2549 a         4987 c         5064 g         2806 t
ORIGIN      
        1 cccgaccacg gcgagggcca gcgggcgccc ggacccgcgg atcagaaggg ccggcggcac
       61 cgatacgagc agaacgacca aagccgctcc cggcatcgcg gcgatcacgc tgagccaggc
      121 ggcgagcagc acacccccgg ccaggaagcc ggccttgggc cgaagcaggg tgagcagggc
      181 cgagaccacg gtgagaagcg cgaccgaggc cgcatcggcg gcaccactga cgatcatccc
      241 ggtcgccacc atgccgcaga ccgcggctgc ggcggcaatg cgaccgagat cgaggccgcc
      301 gtcggcgaag gaagtcagcc ctgccccgcg ggaattgccg tccggcaccg accggtcgag
      361 ttccggcagg gcatcctcca gtgcgtggcc aagttcctcc agtccgggcc ggaatccggg
      421 ttcgcgatcc aggcaggcgt cgacgacttc ggtcatttcc gccggcaggt ccggccgcag
      481 gtcggccagc aggggcaggt ccctttcgat cgcccgcaag gcgagggtcg gggtcggccg
      541 gcgccggggg ttctccccgg tccaggcttc aaagagggtc agggcgagcg agtagacgtc
      601 cccggccggg gtcaccgggt tgccttccgc cgcttccggg gacatgtagg cgagcgtccc
      661 gaggatgtcc ccggtattgg tcaggtcatc gccgtcggtc agccgggcga ccccgaaatc
      721 catcagtttg gcgccgcccc ggccgttcgg gacgatcagg ttggctggct tgatgtcccg
      781 gtggatcacc tcgcgggaat gcgcgtgatc aagcgcttcg cagatgtcga tcccgatctc
      841 cccgatttcc cggtcgctga tcaggtcccg gtccatcagc ttccgcaccg tgtgcccgtc
      901 gaccagttcc gaaacgagat aggcccggcc gccttcccgg ccgtactcgt ggtgggcgaa
      961 ttcgtagagg gtgacgatgc cggtgtggtt gagccgggcc gccgccttcg cctcccgctg
     1021 gatccgggga ccggactcgg cagcggtctc gatcaccttg accgcgacct cgcgctccag
     1081 cctgcgatcc caggcgcggt agaccgtgcc gaatcccccg gcgccaagcc ggtctccgat
     1141 ctcgaatcgg ttgagcacga tcgttccgtt catcactcag cacttttcgg cgcgaaagcc
     1201 cccgaccctc ccctttcggg caccattgtg cggtttcacc ccgtttcggc cgggatttcg
     1261 gtgaactccc tgcaaattgc cgtgcggggg tcgattttcc ggtaaaggga atagattggc
     1321 catgccgaaa gtcgttcggc aggtggactt ttggagtttt cgaaccctta caacccagac
     1381 gaccgcgaaa acgatccttt ctccgaaggg gcgtccgaca accccttcga ggacgatcgg
     1441 acgatcgtgt cggagcccga ttccgatccc gacctggaac tcgatgatcc cggcattccg
     1501 cccccgcctc cccggcgtcg ttcgggcggc ggttccggcc gtggcggacg cccgggtggc
     1561 cgcggtcccc gcggtggcgg ttccggtggt gaccggcagc agttgatggt ccgccgggcg
     1621 atcgcgctcg gtgccggcct gctggtcctg atcctgctcg tgatcggagc gaagggctgc
     1681 ctcgatgccc gcaagaaccg ttcgctcgac gattacgcga acagcgtgac ccagatcgtc
     1741 aacgagacca atgctctcgg caaatccttc ttcggccggc tcagtgatcc gggcgagctc
     1801 tcggtgaccg agttcaccag cgaaatccag tcggaccgtt cggcgatgga cggcttcctc
     1861 agccgggtcg agaagctcga cacgcccggg gacatgagcg ccgcccagga ttccctcacc
     1921 ctcgtctacc agcttcgcgc ctcggcgatg gatgacatcg ccgatcagat gagcaccgca
     1981 ctcggggacg aaggcgccga gcgggcccgg cgccagatcg ccgcccagat ggaagtgctg
     2041 agcgccgccg acgtcctcta caaccgggtt acccgccacc agatcgacaa cacgatcgcc
     2101 aacaacggcg ccagcgcccc ggcgatgccg gtcagtacct tcatcgagga tccccaggac
     2161 tggatcacgg ttgacgcaat caacggcgcg ctcgacgacg tttccggctc caccagtacg
     2221 acggcacccg atgacgggct gacccacggc accggaatcg acccggcctc gtcggtcacg
     2281 gtggatggca ccacgctcag cccggacacc acgacgacga tcgcggcaag caccgacccg
     2341 accgtggaag tcaccgtcat gaaccagggc ggagcgaccg agaccgatgt gacggtcggc
     2401 gtctccgtgg acggcggcac cagcctggaa gccaagatcc ccgagatcgc agccggttcg
     2461 accggcaccg cgacgattcc gctgactccc gctccgaccg gtgaagtgac cctggacgtg
     2521 tcggttgacg ccgtccccgg tgagcagttc accaccaaca acgaagcttc ctacaccgtc
     2581 agcttcgggt aggcacgcca tgcgtgtcgc gtatctcggc cccgccggca ccttcagcga
     2641 ggatgcgctc gaggtcagcc gcttcgcgcc cgagggcttc gaggcggtgg tttgcgaaac
     2701 gattccggcc gcgatcgagg cggtggcggc cggggatgcc gaccgggccc tggttccggt
     2761 cgaaaactcg atcgaggggt cggtccgccc cactctcgac ggcttgatcg agcacgccga
     2821 tgcggttcgc atcgccggcg agttcgacca tgagattcgt tcggccctga tcgcccggac
     2881 ggcccttgac cccggatcga tcgaggtggt gatctcccac ccccagccac tggcccagtg
     2941 cgcgcttttc ctcagaacgg aactcggcca ggccgagcaa cgggtcgcga ccagcaccag
     3001 tgccgcggtt cgcgaggtcg cggaatcgac cggaccgtgg gccgcccttg ctcccgcccg
     3061 cgccgcccgg atttacgaat gcgaggtaat ccgggagggc atcgaggatg aaccggggaa
     3121 tgtgaccaga ttcctctggc tggcccccga atctgccgag aacccggtgg tcggggatca
     3181 gggcggccag tggaagacca gcctcgcctt cgctgaactg ggtgccgatc acccgggtgc
     3241 gctggttgaa gccctccacg aattttccga ccgccgcgtg aacctggtcc ggatcgagtc
     3301 ccgcccgatg cgccgcgaac tcgggcgcta ccgcttcttc atcgacatcg aaggccgcga
     3361 ggacgccgac gatgtcaccg ccgcaatcgc cggcctcagg tcaagagccg agtgggtaag
     3421 agtcctcggc tcctaccccg ccaactgact gcaagtcgcc atgttgagtg acccggggct
     3481 actcctcagt ggcttcgagt tcgggttgct cgggggcttc ttcgccttcc gggtccccgt
     3541 tctcgccgtt ggcggcttcg accaccaggg cgacggcgct gatccggtcg ccgtccttga
     3601 cgttcatgac cttgacgccc tgggtggcgc gaccggtctt gctgatgccg cccgccccgg
     3661 tccgctggac cattccgttc tcggtgatga acatcagctc ctgatgctca cgcacgatca
     3721 gggcgccggc caggccaccc ttggcctcgg tcagcttgat cgtcaggacg cccttggtac
     3781 cgcggttctt gaccgggtac tcggagatgc cggttcgctt gccgtaaccg ttttcggtca
     3841 cgacgaggag gtccgaatca tcccgggcca cggtcagtga cagcacccgg ttgtccttct
     3901 ggcttacgtt catgccgcgc acgccggcgg tgccacggcc catcggccgg gcctgatcct
     3961 cgtggaagcg cgaagcgtgg ccggacttcg agaccatcag caggtcatct tcgccggagg
     4021 tcagctgaac cccgaccagc tcgtcgccct cgcggatctt gatcgcgatg attccgtcgg
     4081 ccttgatcgg cgtgttgtag tcgaggaact cggtcttctt gacctggccc tgcgcggtcg
     4141 cgaagacgag gtacttgttc tccttgaact ccctggtcgg gatgaccgcc atcacgcgtt
     4201 cgccttcccg gaggggaagc aggttgacca gggccgagcc gcgggcctgg cgcgagccct
     4261 cggggagctc gtagaccttc ttgcggtaga ccttgccgaa gttggtgaag aacagcagga
     4321 agtcatgggt cgagcagatg tggagatgct cgatgtagtc gccttccttc aggttcattc
     4381 cggtgacccc gaccccgccg cggcgctgct ggcggtagga gtcgaccggc aggcgcttga
     4441 cgtagccggt gttggtcagc gagatcacca tctgctgctc ggcgatcgtg tcttcgatgt
     4501 cgatgtcccc gccgaagtgc tggaactcgg tccggcgctc gtcgccatgg gtctcgacca
     4561 gttcgagcag ctcgtcccgg accaggccgt aaacccggtc ctcgtcaccg aggatcgccc
     4621 ggagctcgcc gatcagttcc atcagttcgg catgttcctc gcggatcttg tccgactcga
     4681 gcgcggtcag ccgctgcagc cgcatgtcga gaatggcctg ggcctgttcg cggctcaggt
     4741 cgaattcgct gatcagcccg tcacgggcaa catccggatc ggacgagccg cgaatcaggg
     4801 cgatcaccgc gtcgaggttg tcgagcgcga tcagcaggcc ttccagaatg tgggcccggg
     4861 cttcggcgcg gcgcagtttg tactgggtcc gcctggtcac cacctcgcgc tggtgctcga
     4921 cgtagtggac gatcatgtcc ctgatgccga gggttcgcgg caccccgtca accagcgaga
     4981 ccatgttcat tccgaacgag gtctggagct gggtcttctt gaagaggttg gcgagcacgg
     5041 tgttcgggac cgctccgcgc ttgagctcga tcaccaggcg catgcctgtc cggtcggtct
     5101 cgtcccgcag atcggagatc ccgtccagct tcttgtcccg gaccagctgg gcgatcttgg
     5161 cgatcacgcc gctgtcgccg cccttcttca cggtgaacgg aagttcggag acgatcaggg
     5221 cgtccttgcc gcccttgagc ggttcggagt ggatcctcgc gcggaccttg accgagccgc
     5281 ggccggtctg gtaggcgtcg cggattccgc ccggatccat caggccgccg ccggggaagt
     5341 cggggccctt gatgtgcttc atcaggcctt cgagatcgat ctcccggttg tcgatgtagg
     5401 cggcgaccgc gccggcaacc tcggtcaggt tgtgcggcgg gatgttggtc gccatgccga
     5461 ccgcgatccc cgaggaaccg ttgaccagca ggttcgggaa acgggacggc aggaccagcg
     5521 gctcccgctg ggaaccgtcg tagttcgggc cgaaatcgac cgtatccgag tcgatgtcgc
     5581 gcagcatctc ggtggcgagg cgggtgagcc tggcctccgt gtaacgcatc gcggcggcgg
     5641 cgtcgtcatc gatcgagccg aagttgccct ggccgtcgac cagcgggtag cgcagggaga
     5701 agtcctgcgc catgcggacc agggtgtcgt agatcgccga gtcgccatgg gggtggtact
     5761 tgcccatgac ttcaccgacg atcgtggccg acttgcggta cggccggttc ggctgcagac
     5821 cgaggtcatg catgccgtag aggacccggc ggtggaccgg tttcaggcca tcgcggacat
     5881 cgggcagcgc gcgaccgacg atcacgctca tcgcgtaatc gatgtaactg gagcgcatct
     5941 ccagttcgag ctcgcgctct tcgatgtgtc cggtcaatgc ttcgagtgcc atcaggcggg
     6001 tgctccgttc gtggtcagaa ggacgcgatc agatgtcaat gcccgcctcc ttggcgtgtg
     6061 cctcgatgaa ctcgcggcgt ggtgcgacct gatctcccat cagcatcgag aacacctggt
     6121 cggccgccgt ggcgtcttcc agggtgacgc gctgaagcgt cctggtcgcg gggtccatcg
     6181 tcgtttcgcg aagctggtcc gcgttcatct cgccgagacc cttgaaccgg gtcaactgca
     6241 cgccgtgctt ggccaggtcg agcacggccc ggtgcagatc gagaaagtcg ctggcctctt
     6301 cggcccgctc gccgagcgtg acccggaagg gcggctggcc gaaggtaccg agcagctcct
     6361 cgtgaaccgc ggcgaaccgg cggaaggcat caccttcgaa tagccgggcc ggaagcgtgt
     6421 ggttccgggc caggccggaa cgctggtgga tcgccttgat gccggtgtct tctccgtcct
     6481 gggaaaggat ctcggtcgcg aagatgtctt cctcacggtc gacctcaccg agaatgcgcc
     6541 ggacctcgtc aaccgtggtc gcagcttcgt cgagcaggcg ggaacccgcg atgaagctga
     6601 tcacgtcatt tccgaactcg gcctgcaggg ccgcatgcca gccttcgtac tccttcgacc
     6661 gccgggtgta ggtctgccag cggcggctgt tcagcttggt ttccttgccg gtcgagtcag
     6721 tgaccaggaa atcctcgagc cggtcgcgca gcagcagttc ctccagctcc gactccttgt
     6781 cgaggtaggt ctcctggttt ccgcgcttga ccttgtagag cggcggcttg gcgatgaaca
     6841 cgtagccagc gtcgatcaac ggctgcatct gccggtagag gaaggtcagg accagggtcc
     6901 ggatgtgggc gccgtccacg tcggcgtcgg tcgccatgat gatcttgtgg tagcgggcat
     6961 tggtgatgtc gaactcgtcg tggatgccgg tgccgatcgc cgtgatcatc gcctggatct
     7021 cgttgttctg caggaccttg tcgatccggc tcttctcgac gttgatgatc tttccgcgga
     7081 gcgggagcac cgcctgggtc gaacggtcgc gggcctgctt ggccgaaccg ccggccgagt
     7141 caccctcgac cacgaagatc tcggcgagtt ccgggtcacg aaccgagcag tcggccagct
     7201 tgccgggcag gttcatgttc tccagggccg agccccggcg ggtcatgtca cgggccttgc
     7261 gggcggccat gcgggccctt gatgcctcga tcgccttggt gatgaagagc cttgcctcgg
     7321 ccggattttc ctcgaggaac tcggccagct tgcggttgac cgtctcctgg accatcccct
     7381 tgattccggg gttgcccagc ttggtcttgg tctggccttc gaactgggga tcgtggagct
     7441 tgaccgagat caccgccgcc aggccctcgc ggacatcctc gccggccagg ttcgcgtcct
     7501 tctccttgag caggcccttg ctgcgggcgt aggcgttgag ggtccgggtc agggcggcct
     7561 ggaaaccgga gagatgggaa ccgccctcgt gggtgttgat gttgttggcg aagctgtgga
     7621 ttgaatcgtt gtaggacgcg ttccactgca tcgctacctc gacctgaccc tctttttcat
     7681 cctcgccctc gaagtacacg accttcttgc cgagcggctc cttgttttcg ttcaggtagc
     7741 ggacgaagtc ctcgatgccg ccgtcgtact tgaactcgac ctcctggcct tcggcccgtt
     7801 cgtcggtgat ctggatccgc aggccgcggg tcaggaaggc ggtttcacga agccgttcga
     7861 gcagggtctg gaaatcgaag tcgagttcct cgaaaatctc gtcgtccggc aggaaggtga
     7921 tctgggtgcc cgtctctttg gtcgcctctc ccttttcgag ttcggtgacc gggcggccgc
     7981 gctcataacg ctggttccag acgaagccgt cgcgacagat ccggagttcg agccagctcg
     8041 aaagcgcgtt gaccaccgaa gagccgacgc catggaggcc gcccgaaacc ttgtacccgc
     8101 cgccgtcgcc gaacttgccg ccggcgtgca ggacggtcag caccacttcg gccgcggggc
     8161 gaccctcctt ctccatgatg tcgaccggaa tgccgcggcc gtggtcggtg accgtgcagc
     8221 tgttgtcggg atggaggttg acctcgaccg actcgttgtg cccggccagg gcctcgtcga
     8281 ccgagttgtc gacgatctcg taaaccaggt gatggagacc ccggggtccg gtcgagccga
     8341 tgtacatacc cgggcgcttg cggaccgcct cgaggccctc gaggacggtg atgtccttgg
     8401 ccccgtagga ggcgtcgccg ctgctctttt tctcagccaa tcagtgcctt tccaaaaact
     8461 tttcggcggc caccgccgta ccggctacct gtggagaagg tgcgcccggg gggaaccgcg
     8521 agtcaggtct ccataatacc attcccggtc ggataaaccc gcctgccaga gccaaaaaag
     8581 ccctgcaaaa gcgatgtttt tgcacttatg tcagtgactt gttgcacttc gggctcatct
     8641 ggcagcccga aatcgcagtt tttcgggtac cggaccgtcg atccgatcct tgagccgggc
     8701 caggatccgg ggcgccatca gctccagttc ctgggcccag acggcactcg aacactcgac
     8761 tgtcacgacc ccttcgcgct cgtcaatcac gtcggttacc cccgcgatcc gggggcccac
     8821 gacctcgtcc cattgcgtct gaaccgcagc caggagggtc ggcggggtga cctcgccccg
     8881 gaaatcaccc agcacttccc ccagccgctt cggggagggg ctcctgccgc gtgcgtctct
     8941 cacgaagtcc ctcccccgga cccggcgccg gggtcctccc cgttgtcccg ctcttcatct
     9001 gcgccgacca ggtccctgat cgcgacccgg gagatctcct gccgctccgg gatcaactcg
     9061 ccctcggccg cggtgatcac gctctggcca cccccgagca ggcgctcgac cagacggcct
     9121 cgccggtggg cgtcgagttc gctcatcacg tcgtcaagca acatcagcgg cgcggcctcg
     9181 ccggcatcga gcagcgccgc ccgctcggcg aacagcaggg cgaggaggcc cagccgctgt
     9241 tgcccctggg atccgaaacg cctgagctgg cggccgtcga gctccagcct cacctcgtcg
     9301 tggtgcgggc cccagctgct tcgcccgagt cgcaggtcat gctccagccg ctcctcgagg
     9361 ccggcttcga tctcctcctt ggtggtggcg gatccgggcc ggtaactgat ccccttcggg
     9421 ccttccaggc cgaggtcatc agccgcctcg acccagcgct cggccagcgc ttcaaccgcc
     9481 gcttcgcgac ccgaagcaag gttcgcgccc gcttccgcga actgtgaatt ccacgtggcg
     9541 agttgagaag aattggcttc agcggaggaa atgcgggcga tcagggcatt cctctgcgcg
     9601 aggatccggc cgaaagtgga tcgcaacccg gcccggcttg gccaccgggc ggccacgaaa
     9661 ctgtcgaggt gcgcccgccg gcccgccggg gggcctttga ccaactccag ccggtccggc
     9721 gagaagacgg tcaccttcgg ccggtggagg accgcttcgg cctgctcgat cctggctccg
     9781 tccatggtga agcgattgcc ctccgagcgg gaagccgagg ccatgaagac gtggtcgacc
     9841 ccaccgccct ggacggtgac ccggcaccgg gcaaaggctg aaccgaaggg gatcaggtcc
     9901 cgccggtcgg aggtcctgaa cgagcgcgag gtcagaccga agtagatcgc ttcgacgagg
     9961 ttggtcttgc cggctccgtt cggaccgatg atcacggtca ggccggggtc gggttcgaaa
    10021 atcaccccgg ccagagatcg gacctggtcc gcctcgaccc gggaaacgat cacgtgccta
    10081 ctcgttgaga cggatcggca tgaccaggta gcggaagtct tcactgtcga ccggctgcag
    10141 cagaccgggc cgcaggggag aaatcagtcg cagcaggact tcgtcaccgg gaatcgcctc
    10201 gagaccgtcg cggaagaact cgtggttgaa accgatcgcc agttcttcgc cgtcgaagtt
    10261 cgccggcatc ttctcctcgg cgtcgcccag gtccggagtc tcggccgaga tcgtcagctc
    10321 gccgggggtg agggcgatct tcaagggccg gttcttctgg gccatctggc tgacccggcg
    10381 aaccgattca aggagttccg tccgcggcag ccgcacgtcg tgatcccagg tttccgggaa
    10441 gagctgctgg tacttgggga actggccgtc gaccgtgttg gtcgaaagga tcgagccgtc
    10501 gacgttgaag acgacgagcc ggtcggtcac cgagaggcga accgaatccg attcggaagc
    10561 cgaaagcagg cgcgagagtt cacgcagtgc gttggcggga atgttccggt caagttcgac
    10621 cgaaactgct tccgccaggt gagtctgctt tacggcgagc cggtaggagt cggtggccac
    10681 catggtcagc tcgtttccgg aagcgagcac ctggacactg gtcagcaccg ggcgcatgtc
    10741 atcgcgggag gcggcacggg caaccagctc gatcgtttcg agcagtgact cccggccgag
    10801 cgagatcgtt tctccgctgg cgtcgggaag gggcgggaaa tccttctcgt tgagggtgcg
    10861 aaggttgaag ctcgaggatc ccgacttgac gctcaccttc cgctcggaag ccaggtactc
    10921 gatctcggac tgtccggacc cgagcagacg ggaaatgtcg gcgaagagcc ggccgggaag
    10981 gaggacggtg ccgtcggact caaccgaccc ctcgacatgg acgggaaccc gcaggctcag
    11041 gttgctgtcg gttgaactga aggagaggcg cccgtcggtc gcctcgacca ggattccgcc
    11101 aagagccgga ttgactccac cggtcgcgag cgtcctggcg accagggcga gtttgcctga
    11161 aagctcgttg ttcttgatcg agaacttcaa atccgattac ctcgtttaag tttggtagtt
    11221 ctgtggagta aagcgcagaa ccggtgaaag tggcttgggg aagccaaaat cactgtgggg
    11281 agaaatgtgg aaaaaaggat tctttcagcg cgttcggcgg ctggaaaaga catctcagga
    11341 gttttccccg gggctgtgga tttgagcccg gagaatttcg acggcgttgg tgatctcggc
    11401 gtcctggcca accgactgtt cgacccgctc gatcgagttg agaacggtgg tgtggtcccg
    11461 gtccccgtag agcccgccga tctggggcag ggaaagcgag gtgatctccc gggtcaggta
    11521 gatcgcgaca tgcctggccg tggcggtgtt ccggtcccgg cggcgggact ggagggtgtc
    11581 agcgtcagtc ctgtaatggg cggcaaccgt gctgcggatc gtctccggcg tgatctggat
    11641 ggccggagca gattcggtcg cgggaaagac ctgggtgatc aactcggagg tgatcggagc
    11701 ggaggtcagc gaagattcgg caatcgtacg ggtcagtgct ccccggagct gacggaggtt
    11761 ggactgcacc cggcgagcca gcagggtaag ggcgtcctga tcggctacct cgagtccggc
    11821 ttcggtaacc aggtggcgaa gaacggtgag gcgtgtagcc aggttcggtg cttcgatcgg
    11881 caccgtgagg ccccattcga aacggtcgct gagccggtcg gcgagggtcg agatctcgcc
    11941 tggcgagcgg tcagcggaga gaacgatctg gctgccggcg tcatgcaggg cattgaaagt
    12001 gtggaagaac tcttcctcgg tgtggggctt gccggcgatg aactggatgt catcgatcag
    12061 cagtacgtcg agattgcggt agcgccgttt gaactcgtcg gttccctggt cgcggagggc
    12121 ggtgacgaat tcggcggtga aggactcggc gttcgtatag aggacgttca gttcgggaac
    12181 gtgaagagcc aagtagttgg cgatcgcacc gagcaggtgg gtcttgccga ggccgggggg
    12241 accgtgcagg aagagcgggt tgtatgactc ggaaggggct tcggcgaccg ccagggcagc
    12301 ggcgtgggca agatgattgc cggggccgat cacgaagcga tcgaagtcat gggtcgggtt
    12361 gggaccggcc ggccgggtcg gcgccgggac ccgttcgggt tcgggtggat tctcgaagct
    12421 gaccgcggtg aggtgcgaac tcgagtcgac gagagtctcg gttatgacct cgatgtagcg
    12481 ccgctcgacc caggtacgaa tcggatccgg agcctggaga tagagcgtgg ttccgcggac
    12541 cgcgacggga gtaaccggtt cgatccacat ccgccaggcc gagtcaaaga ggcgccgctt
    12601 gagggtcggc tggatggtct cccattcctt cacggcacga gccgagccgg gaagcgactt
    12661 tcggcttcct gccttgcttt cacggtttga actggtggcg gctctcatgt cggaaaaggg
    12721 ctgatcgagg ctggccgggg aacgctcgct gctcaatctc caccctgcct gccgaccata
    12781 gcgccggtcc atgggaggcc gcgttccgga cccgcaaatc gcgcagaaaa tttcttggag
    12841 ccgggtttcc gcccggcagg aggctggcag cgggcgcagg gcgggaacca tgaggcgcct
    12901 gatcccctta tactgcccgg ctccaatgaa gcgtacgtac cagccaaaga agcgcaagcg
    12961 cgcaaagacc cacggcttcc gggcccgcat gagctccaag ggcgggcgcg aggttattcg
    13021 ccgccgccgc cgcaagggcc gcaagcggct cgccgtctga ccatgtcagc aaacggcgct
    13081 ggttctccca ggcgccgccg gctctcccgc agcggagact tcaaacgggc ttaccgcgaa
    13141 gggtcgtcaa aggcgacccg ttacctcgtt ctctaccggt tcgatcgctc ggccgatgat
    13201 gaatccgaga ttcgcctcgg ggtcagcgtt tccaagaagc tgggcgatgc ggtcacccgg
    13261 aatcgggtca aacgcgtgct gaaggaagcc ttctggtccc aggtggaccg cgatgaagcc
    13321 gatcaggact tcgttctggt cgccaggccc ggggtgggcg aggtaatcga cacggacggc
    13381 cttgccggag ccgaagcctg cgtgaaggaa gtgctcgaac ttggcaaggg cgggaagcgt
    13441 tccacgtgaa acggatcatc ctctccccgg ttcgggctta ccagcgctgg atctcccctc
    13501 ttttgccccg cagatgcaag tacgagccca cctgttcggt ctatgccgtg gaggcggtag
    13561 aacgctttgg cgtcatacgg ggtacagttc tcgcctgctg gcgcatcctg cgctgtaatc
    13621 ccttcagtca cggcggcttc gatcccgtgc gggatgaatt cactttgaag gtcggtccga
    13681 tggacccggc cgtctaccac ggagaaaccg aacagcgttg atgccgatcg ccaacatcct
    13741 gcagccactc atcgatgtgg ccaacgcggt tctgtcgttc ttccacgacg atctcggcgc
    13801 cagctggggc atggcgatca tcctgctgac gatctgcacc cgcgccgtgc tgatcccgct
    13861 gacctaccgc cagctcaaga gcatgcgggc cctgcaggct ttccagccgc agatgaagga
    13921 actgcaggag aagtacaaga acgaccgtca gcgcctctcg caggagatga tgaagttcta
    13981 ccaggagaac aagatcaacc cgcttgcctc ctgtatcccg ttgctggccc agttgccggt
    14041 cttcatcacc ctcttccagg ctctgcgcgg cccgctccgg gaagacatct gcggtcagac
    14101 cgcggtcgcc tgttcacagc tgacccaggc ccagatcgac gccaatcccg ccattcatgc
    14161 gctttacccc ttcggcgagt cgttcctctt cattcccgac ctgaccgaca aggcgaccgg
    14221 cggcgtgctg atcgcgctgc tcgtcctcta cgtcggaacc cagctgatct cgggcgtcat
    14281 ctcgatgacc accggcgatc gcaaccagcg catgctgatg ctggctctcc ccttcatctt
    14341 cgttcccttc atcatctcct tcccggccgg cctgatcctc tactggatca cgaccaacgt
    14401 ctggacgatc ggccagacct acgtgatcaa ccgggtcatc cccccgccgg tgatgtccac
    14461 ccctgaaggt gtcgtcgtcg ccgcggcggc gaagcccccg cctccgcccc ccaagaagaa
    14521 gaagcgcaag cgctgagcgc ctgctttctc cgggtagact gcgagcgatg gaagcgagcg
    14581 aatggccggc tgaaccggca gaacgggtgc gactcctggt cgagcgggtt ctcgacgagc
    14641 tcgacctcga cggcgaggtt gaaatggacg agaccgatga cgtcatcacc gccacggtgg
    14701 agggtgacga ggactacggc ctgctgatcg gccgtcgcgg ccagaccatc gacgcgctcc
    14761 agcttttggc tttccaggcc gctttccgcg ggatccgcga acgcaagcgg gttgtggtgg
    14821 acgcagccgg ctaccgggcc cggcgtgagg aagtgatctc cgagcgggcc gatcgcgccg
    14881 ccgagaaggc gatcgcctcc ggctccgagg tggaactcga tccgatgagc gcacgcgagc
    14941 gccggatcgt tcacgagcac ctgaagagcc ggcccgaggt cgagaccttc agttccggtg
    15001 atgatccgca tcggcgggtc gtgatcgcac ccctgatcac cgagtgaccc tggccgggtg
    15061 aattcggtca cccctccacc cccgcccgca ggcccctccg ccggcgaagg cacgaccggc
    15121 ggcgaagcag agccctggct ggaccgggag gccctggccc gcctggaccc gatgctcgac
    15181 ctgctcgcgg agagccatgt ctcgctcagt tcggtgaccg atccggccga agcccgccgg
    15241 gtccacgtgg ccgacagtct ctcgggcctg gcctgccccg aagtcagggc agcgaagcgc
    15301 tgcgcggacc tcggtgccgg agccggcttc cccggcatcc cgctggccgt cttcctgccc
    15361 gaatgcgagt tcgtcctgat cgattccgtg ggccgcaagg tcgatt
//
LOCUS       DAOQKN010000041        14897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_1859_length_14897_cov_5.922113, whole genome shotgun sequence.
ACCESSION   DAOQKN010000041 DAOQKN010000000
VERSION     DAOQKN010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 14897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14897
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1859_length_14897_cov_5.922113"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="PLJ64_04385"
     CDS             complement(<1..300)
                     /locus_tag="PLJ64_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92759.1"
                     /translation="MTTGSIDVHSHYIAPAYRSALESVGKDRIGGIPVPPWTPELAIG
                     FMDAHGIARQVLSVSDPGVDFLAGEAAAKLARECNDYLAGVVASHPDRFSGLAVVS"
     gene            complement(301..969)
                     /locus_tag="PLJ64_04390"
     CDS             complement(301..969)
                     /locus_tag="PLJ64_04390"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC92760.1"
                     /translation="MTVHTSSIPEKNDTKPPGRVERRKAKTRASLIEAARRLFASKNI
                     ESTSVAEIAEEADIAVGSFYNYFETKDELLAALLESTLVEQLARIRARQSQTDDPAEK
                     VAIGHRHLVDLATREPDLAWLIVRFEVPQRIASLGLAEAARADMMEGIATGRFDVRNP
                     ELSLRASGGALLGVIHTILLGELPGDSGGDLAAGVLMGLGMKPAEAREVAARPLPPES
                     LEAE"
     gene            1031..2821
                     /locus_tag="PLJ64_04395"
     CDS             1031..2821
                     /locus_tag="PLJ64_04395"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HNC92761.1"
                     /translation="MNRVPVLVVGGGGAGLTASMLLANLDIEHLLINSRPGTSDLPKA
                     HVLNLRAMEILDDVGAADRISEKSTPPEAMAATAFYAGFGGDDPDAGRRIAKLQSWGA
                     GGTDGNWREASPMIQRNLPQIRLEPLLRERAEELSPGRIRFNHELTGLEQSGDAVLAT
                     VRDNDSGEQYEVTADYVIAADGGRTVPGLLGIEYEGLGTVSETATLHVSADFSDLAID
                     DDVLIRWTISPVSGAGVVMVPMGPEKWGPESEEWVIHIQYLVGDPREITDEEVLADVR
                     SAMEIGELPMEIHKISRWTVEAVLASSFRSGRVFLVGDCAHKHPPTGGLGLTSAIHDV
                     HNLTWKIASVLKGEAGESLLETYEPERRPVVARNAQRSLENAINHLGTMAEIGVTPEN
                     STEENWAAMKRLWNEDPADQEFREKVARGARSQSMEFNELNVEYGYAYDSAAVVPDGS
                     PPAPNPDEVRIYLPSTRPGSPLPHAWLDDLEGNRQPIKDLVRPGRFLLIAGEEGDEWL
                     QAANDLAGSAKVPLDSLRIGHIDGDLFDPQLTWIRNRGIEPDGAILVRPDRFVAWRSM
                     SSASNPGQTLAKALTSVLGREVHMPEGAAA"
     gene            2818..3702
                     /locus_tag="PLJ64_04400"
     CDS             2818..3702
                     /locus_tag="PLJ64_04400"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNC92762.1"
                     /translation="MSAVSKIGHMAMRVSDLDRAVEFQSEVMGLVETERQGGTSFMTC
                     NDRHHELILIQSGKRGYDHIGLEVATAEDLEAVRAQAAAAGGDVLTQIQEGETGIDRC
                     FFVRSPAGHVFKIFLGMEKVAEPEPGDRPSQFEHVSTKALRMGREERFLSEGLGFRFS
                     DRLGRTASWWHCDPDHHGMAVVIAPRAELSHYAWSLEDLNALGRTADRLRARGQRAIW
                     GPSRHGPGNNLFLYFHDADGAMIECCAELEKMPPEGDYKPRKWTGGMKDINQWGSTPP
                     AKFLLTGFPINHREGEVK"
     gene            3699..4700
                     /locus_tag="PLJ64_04405"
     CDS             3699..4700
                     /locus_tag="PLJ64_04405"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNC92763.1"
                     /translation="MKLRSGPEGLMVEAGPGEWVSLPALKREATELDLDDGQTTDMIA
                     FLAAGEPARKAVQKSLDSVDADKVKADPDQAGLPLRPRSMRAFMLWESHMIASSRVLV
                     KRFFPPPAAKVVAGFEKVTGKTFPKLKPNRRFYEKPTFYVANHAAVIADGDPMWWPSH
                     TKFLDFELELAFVIGKPIADASPEEAADAVGGWFVLNDWSARDVQADDARENIFGPVV
                     KSKTFANSIGCDVITPDELPDWQEATGRVRVDGELWCEGTTAGAAHDIGAMLAYASAG
                     ECLMPGDVISTGTVPGCCGLELDRWIQPGQTVELEIDGIGTLTNRIDAEARPVSIAG"
     gene            complement(4729..5934)
                     /locus_tag="PLJ64_04410"
     CDS             complement(4729..5934)
                     /locus_tag="PLJ64_04410"
                     /inference="COORDINATES: protein motif:HMM:NF037653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC92764.1"
                     /translation="MSWPKPSERTAELIRKCVNRILEDSEGILEAVNEASIPDEVAGV
                     GSDPVLLRNYRRVNEATVLQWAEANLREPGCEVPPYSGSEVTALTRDLVRRGLDTNAL
                     EPYRAGQNVTWQKWMEAAFDETDDPDELRELLEVSARSIFAYVDSTMMLTADRIQQQR
                     EEFNQTGAERLETVVLLLEDNPIDPHRAAARLNYSFEGTQLAAVIWKEETPDADALEV
                     GAERLKQAAGSAKLLAVHATSAALWAWVKPTREFSEIEAGLAGDGQIRFALGTPLPGL
                     DGFRRSHADASEVQRLVLRLDSGLEVASYEAMKVAALLTHDEERSKRFTAETLGELAD
                     GPVNLKETVRTYLRNQSNATRTAELLFAHRNTVLARIAKADELLPSPLSESSFEVAAA
                     LEILHWQES"
     gene            6118..7629
                     /locus_tag="PLJ64_04415"
     CDS             6118..7629
                     /locus_tag="PLJ64_04415"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNC92765.1"
                     /translation="MDLDSSSQDELDVVIIGAGVSGIGAAYQLQKQHPGKSYAILESR
                     NDLGGTWDLFRYPGIRSDSDLYTFGYQFKPWTDEKAIADGPAIKAYINETAVENGIDK
                     RIRYGHKVVRAEWSSDQARWTLTVELADTGETFEMKAKWIFAATGYYRYDEGYMPEFK
                     GIADYAGEVIHPQHWPEDLDFADKRIVIIGSGATAVTLLPELAKEADHVTMLQRSPTY
                     ILPVPEKDRLGLALRRMIGDMRAYRLIRRKNILRQKFVYQFSQRFPKAARAFIRRVNR
                     KELEGSGCDVDVHFNPSYDPWDQRLCAVPNGDLYKSLRNGSASVVTDRIDTFTESGIR
                     LESGKELEADIVITATGLNVQLFGGIEVTIDGETFDAPDTVAYKGLMLSDVPNLVFAV
                     GYTNSSWTLKIDLVCEYFCRLLDHMDRTGAEIAVARVTDPEMERIPLLDFPAGYVLRA
                     LDSLPKQGTKEPWYLAQNYAKDSRFLREGNVADEYMEFRQIERKSTAKEEIPA"
     gene            7626..8537
                     /locus_tag="PLJ64_04420"
     CDS             7626..8537
                     /locus_tag="PLJ64_04420"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNC92766.1"
                     /translation="MSEVRGKVCVVTGAGSGIGRALALELARRGARAVAISDIKLETA
                     QATAELIGAGTEIHTAALDVSDREAFAAYASEVAGTFGQVHQIYNNAGVADNDPITEF
                     DYDRYERILNVNLWGVIYGTKEFLPHLIASGVGHVINVSSLNGIMAQQGLSAYCASKF
                     AVRGFTESVAFDLKGEGLPVRTTVVHPGGIKTSIADSAMEWARSAGLPVTPEQEARKA
                     FYNEKLLKMDPARAARIVIDGVESNNPRVLVGNDAKMLDALVRLMPRRWPMAFGLLER
                     RFEKELEARGIQPKSKQPVAESRPVHH"
     gene            8534..9889
                     /locus_tag="PLJ64_04425"
     CDS             8534..9889
                     /locus_tag="PLJ64_04425"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNC92767.1"
                     /translation="MSASTDIRLPPGPGSPRRVNGALFLAARIRAFRRWHEQFGDIYF
                     VDIANFGPTVVIRGAELHRKVFTAKANILHAGDNALGRTLGPGSIFSMDEDQHLAERR
                     KLLPPFHGERMRSYESLVEAEANREMDRWPVDEPFPTLKSFNSITLRVILRAVFGAEG
                     KELAELEQIIPPITRYGQMRVASSLLMRDYGRFSPGGRGERLMTRFHEIIRTLIDKHL
                     ADPGLADRIDILALMLNSDLQEGRAVDYQATGEELMTILVAGHETTASSLAWNIERIT
                     RHPEVLDRLTAEARTDQNEYRLAVINEVLRTRPVIGMTGRSVFAESFELDGWKLPAGT
                     RILCNIRETNVDPSAHPEADRFNPDRYVGKKPDTYSWIPFGGGLRRCLGAAFAQMEMD
                     IVLRTLLRRFDLAATSRPPEGERFKGLAYAPSRGGVAMVRYRDGKPPARLEPQVAAGA
                     R"
     gene            10027..11310
                     /locus_tag="PLJ64_04430"
     CDS             10027..11310
                     /locus_tag="PLJ64_04430"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNC92768.1"
                     /translation="MLRAAVASAAIALLVADHYLHMDHWVIICSALVLLVLICGGLVI
                     AIGSARRNVRSEALNRIKEEENLHLADIVRSSKDAIFSVDLDGKVTAWNEGAHNLYGY
                     SAEEAIDRRLTDLTIPAERSKELGATFRRVISGGWQEFESERITKSGERIRISSRTFP
                     VRDGEGNVIGMSISTHDMTGPELPGPEMEQDLLWQSRLREALEKSSFVFWGQPVFDAR
                     SGSVDHTELLIRMDLNGEIIAPEEFLPHAEASGLIDRIDAWAIREGIRIAAGRPVAIN
                     LSGRSVSASETGKIISESLDDSRTDPRNVRFEITETAAIENLDAARSLVVELKKMGCA
                     VSLDDFGTGYGSFTYVRQLPPVDLKIDSSFVMDLDSEEASRRVVSSIVSISKTFGVST
                     VAEGIEDEATLELVKGMGINFLQGFHLGTPQPLDR"
     gene            11353..12312
                     /locus_tag="PLJ64_04435"
     CDS             11353..12312
                     /locus_tag="PLJ64_04435"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HNC92769.1"
                     /translation="MAKIGFTLSSEEQPPGLLVEHAAAAEQAGFDFLSISDHFHPWVD
                     RQGQSPFVWSVLGAVSRATERIELITGVTCPTTRIHPAIIAQAAATTACLMPGRFSLG
                     LGSGENLNEHILGDRWPSVDIRQERLEEAVTVIRSLWEGGLTNHRGVHYEVENARIYT
                     LPDQLPPILIATAGEQATELAGRVGDGLVGLIPDSSVIEQFRESGGEGKPAYAQVHIS
                     WRESKEEAVKTALEWWPNAGVPGNLFVELPLPSHFSEAASTVDEDLIAESVVCGPDPE
                     PIVEEIGRYLDAGFSHVYVHQVGPDTPGFFDFFRDRVLPEFQA"
     gene            12379..13296
                     /locus_tag="PLJ64_04440"
     CDS             12379..13296
                     /locus_tag="PLJ64_04440"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HNC92770.1"
                     /translation="MSPGHPSGRQFEITHGSQSATVVEVGGGIRTYEVEGRSVLDPYP
                     VERICDGAHGAPLIPWPNRLDRGRYSFDGEAYQLPLTEPEQDNAIHGFLRWVTWAAEV
                     HGPDAVTMSTTVAPRPGYPFWLDVSVGYRLGDKGLEVSTEIVNKGERACPVGHGQHPY
                     LSAGSGLIDDCLLQHPGTVRILTDDRQLPAGREEASGTKFDFAEPRQLGDIEIDFAFT
                     DLRRDGEGKAWTRLEGADGKTASIWVDRNYPLVELYTGHTLAPDRARTGLGTEPMTCA
                     PDAFNNGEGLIRLEPGESASAVWGACLEG"
     gene            13299..14705
                     /locus_tag="PLJ64_04445"
     CDS             13299..14705
                     /locus_tag="PLJ64_04445"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF009492.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HNC92771.1"
                     /translation="MAHRQTFLILGASGDLTSRLLLPGLASLVDAGQTGDLLLVGSSV
                     DEWSQEEWRDRVRNSLEKSGGAGEGSMALAASARYVQADVTDVEGVRSVLAAVEGQPI
                     IYFALPPEVSITACEVLRKVGCPPETRLVLEKPFGRDAESAEAFNRLLAEIVPESQVH
                     RVDHFLGLSTVLNIFGLRFANRVFEPMMNSHHVESVEILWDEDLALEGRAGYYDRAGA
                     LVDMIQSHLLQVAAVLAMEPPPTTGEKDIRDGTARILRAASVWDDDPARFSRRARYSA
                     GTIKGRELPAYADEEGVEPEKMTETFAEIVIGVDTWRWAGVPFRLRSGKAIGNPRKEA
                     VINFRDPEQIPRGLVGAEGQNRLRIGLGFGSDTVRFDIAVNGPGDPKELDFATLEATL
                     GAGYLSEYGEVLKTVLDGTPALSVRGDAAVDCWRIIDPVRQAWKTGEVPLLEYPAGSS
                     GPEPWPESGLPGSQPGGS"
     gene            complement(14687..>14897)
                     /locus_tag="PLJ64_04450"
     CDS             complement(14687..>14897)
                     /locus_tag="PLJ64_04450"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNC92772.1"
                     /translation="ESPWVDSYGAGDSFAAGITYGLGLGLPVLEAARIGALCGAANLQ
                     GRGPYAGQATAEDLARFDLAQDPPG"
BASE COUNT         2730 a         4762 c         4864 g         2541 t
ORIGIN      
        1 ggatacgact gccaggccgc tgaagcgatc gggatgggag gcgaccacgc cggcgaggta
       61 gtcgttgcat tcgcgggcca gcttcgccgc cgcctcgccg gcgaggaagt cgacgccggg
      121 atcggagacc gaaagcacct gtcgcgcgat tccgtgggcg tccatgaagc cgatcgcaag
      181 ttcgggggtc cagggtggca ccggaatgcc gccgatccgg tccttgccga ccgactcgag
      241 cgccgaccgg tacgcgggag cgatgtagtg cgaatgaacg tcgatcgatc cggttgtcat
      301 ctattctgcc tccaggcttt cggggggaag gggccgggct gcgacctccc ttgcctcggc
      361 cggtttcatg ccgaggccca tcagtacgcc ggcagcgaga tccccgccgg agtcgccggg
      421 caactctccg agcaggatgg tgtggatcac gccaagcagg gctccgccgg aggcgcgaag
      481 cgacagctcc gggttcctca cgtcaaagcg gccggtggcg atgccttcca tcatgtccgc
      541 tcgggcggcc tcggcgaggc cgagactggc aattcgctgg ggtacctcga agcggacgat
      601 cagccaggca aggtcgggct ccctggtcgc caggtcaacc aggtgacggt gcccgatcgc
      661 aaccttctcg gccggatcgt cggtctgcga ttgccgggcc cggatccggg cgagttgctc
      721 gaccagagtg ctttcgagca gagcggcgag cagctcgtcc ttggtctcga agtagttgta
      781 gaaggagccg acggcgatgt ccgcttcttc ggctatctcg gcgacgctgg tcgactcgat
      841 gttcttcgag gcgaacagtc gcctggcggc ctcaatcagg gaagcacgtg tcttcgcctt
      901 gcgtcgctcg acccgccctg ggggtttggt gtcgttcttt tcagggatcg atgaagtatg
      961 gacggtcatg acaacatgag tatagcgtca aacttgaata agtgttcatg tttgtgctac
     1021 ggtcgtcgac atgaaccgag tacccgtact ggtcgtggga ggcggcgggg ccggattgac
     1081 ggcctcgatg ctccttgcga acctcgacat agagcatctg ctgatcaact cccgtcccgg
     1141 gacttcggac ctgccaaaag cgcatgtcct caacctgagg gcgatggaga ttctcgatga
     1201 cgtcggcgcc gccgaccgaa tcagcgagaa gagcacgccg ccggaggcaa tggcggcgac
     1261 cgccttctac gccggcttcg gcggggacga cccggacgcc ggacgccgca tcgcgaagct
     1321 ccagagctgg ggagcgggag ggaccgacgg gaactggcgc gaggccagcc cgatgattca
     1381 gcggaacctg ccccagatcc gcctcgaacc gctcctcagg gagcgtgccg aggaactctc
     1441 gccgggccgg atccggttca accacgagct gaccggcctc gagcaaagcg gcgacgccgt
     1501 cctggcgacg gtcagggaca acgattccgg tgagcagtac gaagtgaccg ccgactacgt
     1561 gatcgcggcc gacggcggcc gcacggtgcc gggccttctg gggatcgaat acgagggtct
     1621 cggcacggtt tccgagaccg cgacccttca cgtttcggcc gacttctccg acctggcgat
     1681 cgacgacgac gtgctgattc gctggacgat ctccccggtg agcggagccg gtgtggtcat
     1741 ggttccgatg gggccggaga aatggggtcc ggaatccgag gaatgggtga tccacatcca
     1801 gtacctggtc ggggacccgc gtgagatcac cgacgaagag gtcctggccg acgtgcgaag
     1861 cgcgatggag atcggcgagc tgccgatgga gatccacaag atctcgcgct ggaccgtcga
     1921 agcggtcctc gcctcctcgt ttcgctccgg tcgggtgttc ctggtcggtg actgtgccca
     1981 caagcaccct ccgaccggcg gccttggcct gaccagcgcg atccacgacg tccacaacct
     2041 gacctggaag atcgcgtcgg tgctcaaggg cgaagccggc gagagcctgc ttgagaccta
     2101 cgagccggaa aggcgaccgg tcgtcgcccg caacgcccag cgctcactcg aaaatgcgat
     2161 caaccatctc ggcacgatgg ccgagatcgg agtgaccccc gagaactcga ccgaagaaaa
     2221 ctgggcggcg atgaagcgac tctggaacga ggatcccgcc gaccaggagt tccgtgagaa
     2281 ggtggcgagg ggagcgcgct cgcagtcgat ggaattcaat gaactgaacg tcgaatacgg
     2341 gtacgcctat gactcggcag cggtggttcc ggacggaagt ccgccggcac cgaatcccga
     2401 tgaggtgcgg atctatttgc cttcaacccg tcccggttca cccctgccgc atgcctggct
     2461 ggatgacctg gaaggcaacc gtcagccgat caaggacctc gtcagaccag gccgtttcct
     2521 tttgatcgcc ggcgaagaag gcgacgaatg gcttcaagcc gcgaatgacc tggccggctc
     2581 ggccaaagtc ccgctggaca gcctgcggat cggacacatc gacggtgacc tcttcgaccc
     2641 gcagttgacc tggatccgca accgggggat cgagcctgac ggagcgatcc tcgtccggcc
     2701 cgaccgtttc gtggcctggc gtagcatgag ctcggccagc aacccggggc agaccctggc
     2761 caaggccctg acctcggtac ttggccggga agtccacatg ccggaaggag cagcagcatg
     2821 agtgccgttt ccaaaatcgg tcacatggcg atgcgggtaa gtgaccttga ccgtgccgtc
     2881 gagttccaga gcgaagtgat gggtctggtc gaaaccgagc ggcagggcgg cacctcgttc
     2941 atgacctgca acgaccgtca tcacgagctg atcctgatcc agtccggcaa gcggggctac
     3001 gaccacatcg gcctcgaggt cgcgactgcc gaagatcttg aggcggtccg cgcccaggct
     3061 gccgcggccg ggggagatgt tctcacccaa attcaggagg gcgagaccgg cattgaccgc
     3121 tgtttcttcg tccgctcacc ggccggccat gtcttcaaga tcttcctcgg catggagaag
     3181 gtggccgagc cggagccggg tgatcgcccc tcgcagttcg agcatgtttc aaccaaggcg
     3241 ctacggatgg gccgggagga gaggttcctt tcggaaggcc tcggtttcag gttctccgac
     3301 cggctgggcc gcaccgccag ctggtggcac tgcgaccccg accaccacgg catggcggtc
     3361 gtgatcgcac cccgggcaga gctttcccac tacgcctgga gcctggaaga cctgaacgcc
     3421 ctcgggcgca ccgccgaccg gctccgggcc aggggtcagc gggcgatctg gggaccgagc
     3481 cggcacggtc cgggcaacaa cctcttcctc tatttccacg acgccgacgg ggcgatgatc
     3541 gagtgctgcg ccgaactcga gaagatgccg cccgaaggtg actacaagcc ccgcaagtgg
     3601 accggcggca tgaaggacat caaccagtgg ggatcgaccc cgccggcgaa gttccttttg
     3661 accggattcc cgatcaacca ccgggaaggg gaagtcaagt gaagctgcgc tctggcccgg
     3721 aagggctgat ggtcgaagcc ggaccgggcg agtgggtctc gcttcccgcg ctgaagagag
     3781 aagcgaccga actcgatctg gacgacgggc agacgacgga catgatcgcc ttcctcgccg
     3841 cgggcgaacc ggctcgcaag gcggtgcaga agtcgctcga ttcggttgac gcggacaagg
     3901 tgaaagccga tccggatcag gccggtctgc cgctgaggcc ccgctcgatg cgcgccttca
     3961 tgctctggga atcgcacatg atcgcgtcca gccgggtgct cgtgaagcgc ttctttccgc
     4021 cgccggccgc caaggtcgtg gcaggtttcg agaaggtcac aggcaagacc ttcccgaagc
     4081 tgaaaccgaa caggcgcttc tacgagaagc cgaccttcta tgtggccaat cacgccgccg
     4141 tgatcgccga tggcgacccg atgtggtggc ccagccacac gaagttcctc gacttcgaac
     4201 tcgagcttgc cttcgtgatt ggcaagccga tcgcggatgc cagcccggag gaggccgctg
     4261 atgccgtcgg tggctggttc gttctgaacg actggagtgc ccgagacgtt caggccgacg
     4321 acgcaagaga gaacatcttc ggtccggtgg tgaagtcaaa gaccttcgcc aactcgatcg
     4381 gctgcgacgt gatcaccccg gacgaactgc cggactggca ggaggctacg ggccgggtcc
     4441 gggtcgacgg tgaactgtgg tgcgagggta cgactgccgg ggcggctcat gacatcggag
     4501 cgatgctcgc ctacgcttcc gccggtgagt gcctgatgcc tggcgacgtg atctcgaccg
     4561 gcaccgtgcc gggctgttgc gggcttgaac tcgaccgctg gattcagccg ggccagaccg
     4621 tggagctgga aatcgacggt atcggcaccc tcaccaaccg gatcgacgcc gaagcgaggc
     4681 ctgtctcgat cgccggatag gcgggtcgcg acaggccttc aggcggggtc agctctcctg
     4741 ccagtgaagg atctccaggg cggcggcgac ttcaaacgag gattccgaga gcggggaggg
     4801 gagcagttcg tcggccttgg cgatcctcgc caggaccgtg ttccggtgcg cgaagagcag
     4861 ttccgccgtc ctggtcgcat ttgactggtt ccggaggtag gtgcgcaccg tctccttgag
     4921 gttgacgggg ccgtcggcca gctcgccgag ggtctccgcg gtgaaccgct tcgaccgttc
     4981 ttcgtcgtga gtcagcaggg cggccacctt catcgcctcg tagctggcaa cctcaaggcc
     5041 ggaatcaagc cgcaacacca gccgctgaac ctcggaggca tcggcgtggc tgcgccggaa
     5101 gccgtccaga ccggggaggg gtgtgccgag cgcgaagcgg atctggccat cgccggcaag
     5161 acccgcctcg atttcgctga actccctggt tggcttgacc caggcccaga gggcagcgct
     5221 ggtcgcgtgc acggccagca gcttcgcgga accggcggcc tgcttcagcc tctccgcccc
     5281 gacttcgagc gcgtcggcgt ccggcgtttc ctccttccag atcacggcgg cgagctgggt
     5341 cccctcgaag ctgtagttga ggcgtgccgc cgcgcgatgg ggatcgatcg ggttgtcctc
     5401 aagcaggagc accaccgttt ccagtcgctc ggcgccggtc tggttgaact cctcccgctg
     5461 ctgctggatc cggtccgcgg taagcatcat ggtcgagtcc acataggcaa agatcgagcg
     5521 ggccgagact tcaagcagct cccgcaactc gtccggatca tctgtctcgt cgaaggcggc
     5581 ttccatccac ttctgccagg tgacgttctg accggcccgg taaggctcga gggcgttggt
     5641 gtccagtccc cggcgaacca ggtcccgggt cagcgcggtg acctccgagc cggagtaggg
     5701 cgggacttcg catcccggtt cccgcagatt tgcctctgcc cactggagaa ccgttgcctc
     5761 gttcacgcgc cggtaattgc gcagcaggac cggatcgctg ccgacgccgg ccacctcgtc
     5821 cgggatgctc gcttcgttga ctgcctcgag gatcccctcc gaatcctcga ggatccggtt
     5881 cacacatttg cggatcagtt ctgctgtccg ttcggacggc ttcggccatg acattggccc
     5941 agactaacgc tgactgggct aaaaccatgt atttgggaag ggccggtccc tgctttcccg
     6001 taaacctgtg cacttcgcac agcccggatc gaaagtctgg atctttcacc gttgagttgg
     6061 gccggtcgcg gcacgaacat tcactcaatc ggcgatctat cagaaggacc ccggaaaatg
     6121 gatcttgatt caagcagcca ggatgaactt gacgtagtga tcatcggagc cggcgtttcc
     6181 ggcatcggtg ccgcgtacca gctacagaag cagcatccgg gcaagagcta cgcgatcctc
     6241 gaatcgcgca acgacctcgg cggcacctgg gatctctttc gctatcccgg aatccgctcc
     6301 gattccgatc tctacacctt cggctaccag ttcaagccat ggaccgatga aaaggcgatc
     6361 gccgatggtc ccgcgatcaa ggcctacatc aacgaaaccg cggtcgagaa cggaatcgac
     6421 aaaaggatcc gctacggcca caaggtcgtc agggccgaat ggtcttcgga ccaggcgcgc
     6481 tggaccctga ccgtggagct cgccgacacg ggcgagacct tcgagatgaa ggcgaagtgg
     6541 atcttcgccg cgaccggcta ttaccggtac gacgagggct acatgccgga attcaaggga
     6601 attgccgatt acgcggggga ggtaatccac ccgcagcact ggccggagga tctcgatttc
     6661 gccgacaaac gaatcgtgat catcggcagc ggcgcgaccg cggtaaccct gctgcccgag
     6721 ctggccaaag aggccgatca cgtgaccatg ctgcagcgct cgccgaccta catcctgccg
     6781 gtcccggaaa aggaccggct cggcctggcg ctgcgccgca tgatcgggga catgcgcgcc
     6841 taccggttga tccgccgcaa gaacatcctt cgccagaagt tcgtctacca gttcagccag
     6901 cgcttcccca aggccgcgcg ggccttcatc cgaagggtga acaggaagga actcgaaggt
     6961 tccggctgtg acgtggacgt ccatttcaac ccgtcctacg acccctggga ccagcgcctt
     7021 tgcgccgtac cgaacgggga cctctacaag tcactcagga acggctcggc ctcggtcgtg
     7081 accgaccgta ttgacacctt caccgaaagc gggatccggc tcgagtcggg caaggagctc
     7141 gaggccgaca tagtgatcac cgcgaccggg ctgaacgtcc agctcttcgg tggcatcgag
     7201 gtcacgatcg acggcgagac cttcgatgct ccggacacgg ttgcctacaa gggcctgatg
     7261 ctgagcgacg tgccgaacct cgtcttcgcg gtcggataca cgaactcgtc ctggacgctg
     7321 aagatcgacc tggtctgcga gtacttctgc cggctgctcg atcacatgga ccggaccgga
     7381 gcggaaatcg ccgtggcccg ggtcacggac ccggaaatgg agcggattcc gctgctcgac
     7441 ttcccggccg gctacgtgct gcgggctctg gacagcctgc cgaagcaggg cacgaaggaa
     7501 ccctggtacc tggcccagaa ctacgcgaag gattcccgat tcctccggga aggtaatgtc
     7561 gcagatgagt acatggagtt ccgccagatc gagcggaagt caacggcgaa ggaggagatc
     7621 cccgcttgag tgaagtcaga ggaaaggtct gcgtagtaac tggcgcgggg tccggcatcg
     7681 gtcgggccct cgcacttgag ctggcgaggc ggggagcccg ggcagtcgcg atcagcgaca
     7741 tcaagctcga aaccgcccag gcaaccgcgg agttgattgg tgccgggacg gagatccaca
     7801 ccgcagcgct cgacgtgagt gaccgcgagg cgttcgccgc ctatgcgagc gaagtagccg
     7861 gaactttcgg ccaggtccac cagatctaca acaacgccgg cgtcgccgac aacgacccga
     7921 tcaccgagtt cgactacgac cgctacgaga ggatcctgaa cgtgaatctc tggggcgtga
     7981 tctacggcac caaggagttc ctgccgcatc tgatcgcctc cggcgtcggc catgtcatca
     8041 acgtctccag cctcaacggg atcatggccc agcaggggct gagcgcctac tgcgcgagca
     8101 agttcgcggt ccgcggcttc accgaatcgg tcgccttcga cctgaagggc gaagggcttc
     8161 cggtgcggac cacagtggtc catcccggcg gcatcaagac gagcatcgcc gattcggcga
     8221 tggagtgggc caggagcgcc ggacttccgg tgacgcccga gcaggaggcg aggaaagcct
     8281 tctacaacga gaagctgctg aagatggacc ccgcccgcgc cgcccggatc gtgatcgacg
     8341 gagtggaatc gaacaacccg cgggttctgg tcggaaacga cgccaaaatg cttgacgccc
     8401 tggtccgact gatgccacgg cgctggccga tggcgttcgg gctgctcgag cgccgtttcg
     8461 aaaaggaact cgaagcccgc ggcatccagc cgaaatcgaa acagccggtt gctgaatccc
     8521 ggccggtgca ccattgagcg cttccactga catcaggctt ccgcccgggc cgggcagccc
     8581 gaggcgggta aacggagccc tgttcctggc ggcccggatc cgggctttcc gccgctggca
     8641 cgagcagttc ggtgacatct acttcgttga catcgccaac ttcggtccga ccgtggtgat
     8701 ccgcggtgcc gagctccacc gcaaggtgtt cacggccaag gcgaacatcc ttcacgccgg
     8761 cgacaacgcc cttggccgga ccctcggtcc gggctcgatc ttctcgatgg acgaggacca
     8821 gcacctggcc gaacgccgca agctcctgcc gccgtttcac ggcgagcgga tgcgctccta
     8881 cgagagcctg gtcgaagccg aggcgaaccg cgaaatggac cggtggccgg tcgacgagcc
     8941 gttcccgacc ttgaagtcct tcaattcgat caccctgcgg gtcattctcc gggcggtgtt
     9001 cggggccgag ggcaaagagc ttgccgaact ggagcagatc atcccgccga tcacccgtta
     9061 cggccagatg cgggtcgcct ccagtctgct gatgcgagac tacgggcggt tcagcccggg
     9121 cggtcgcgga gagcggctga tgacccggtt ccacgaaatc atccggaccc tgatcgacaa
     9181 gcacctggcc gaccccggcc tcgccgaccg gatcgacatc ctcgccctga tgctgaactc
     9241 ggacctgcag gaggggagag ccgtcgatta ccaggcgacc ggggaagagc tgatgaccat
     9301 tctggttgcc ggtcacgaaa cgaccgcttc ctcactggcc tggaacatcg agcgcatcac
     9361 caggcacccc gaggtgctgg accggctcac cgccgaagcc cggaccgatc agaacgagta
     9421 ccggcttgcg gttatcaacg aagtcctcag aacccgcccg gtgattggca tgaccgggcg
     9481 ttcggtcttc gccgagagct tcgagctcga tggctggaag ctgccggccg gcaccaggat
     9541 cctctgcaat atccgggaaa ccaacgtgga tccttcagct caccccgaag ccgatcgctt
     9601 caacccggac cgctacgtcg gcaagaagcc cgacacctat tcctggattc ccttcggggg
     9661 cgggctgcgc cgctgccttg gcgccgcctt cgcccagatg gaaatggaca tcgtgctcag
     9721 gaccctgctt cgtcgtttcg acctcgccgc aaccagccgg ccgcccgaag gcgagaggtt
     9781 caagggactc gcctacgccc cgtccagggg cggggtggcg atggtccgct accgcgacgg
     9841 caagccgccc gcccggctgg agccgcaggt ggccgccgga gcacgctagg ccatcgcggt
     9901 tatcttttag ctgagcctca gggggcgtcg cccacgctcg tcgatcgagc aattttgaaa
     9961 cggcaaccaa gatccgcatc agtgagggca ctctttcgaa cggatccgtt cggggcacgg
    10021 tttgccgtgc tgcgtgcagc ggtcgcgagt gccgcgatcg cattgctggt tgccgaccac
    10081 taccttcaca tggatcactg ggtgatcatc tgttcggcgc tggtcctgct cgtcctgatc
    10141 tgcggcggcc tggtcatagc aatcggttcc gcccgccgga atgtccgcag cgaagccctg
    10201 aaccggatca aggaggaaga gaacctccac ctcgctgaca tcgtcagatc ctccaaggac
    10261 gcaatcttct cggttgacct cgacggcaag gtgaccgcct ggaatgaagg ggcgcacaac
    10321 ctgtacggct actcggccga ggaagcgatc gatcgccggc tcactgatct gaccatcccg
    10381 gccgagcgtt caaaggaact cggagcgact ttccggcgcg tgatcagcgg cggctggcag
    10441 gagttcgaat cggaacggat caccaagtcg ggagaaagaa tccggatttc gagccggacc
    10501 tttccggtcc gggacggcga gggaaacgtc atcggcatgt cgatcagcac ccacgacatg
    10561 acgggcccgg agttgccggg tccggaaatg gagcaggacc tgctgtggca atcccgcctt
    10621 cgggaagcgc tggagaagtc ttcatttgtg ttctggggcc agcccgtctt cgacgcccgc
    10681 agtggttccg tcgatcacac cgaactgctg atccgaatgg atctgaacgg ggagatcatc
    10741 gctccggagg aattcctgcc acacgccgaa gcttcgggcc tgatcgaccg gatcgatgcc
    10801 tgggcgatcc gggaagggat ccggatcgcg gccgggaggc cggtcgcgat caacctgtcc
    10861 gggcgcagcg tctcggccag cgagaccggg aagatcatct ccgaatccct cgatgactcc
    10921 cgcaccgacc cgaggaacgt ccgtttcgaa atcaccgaga ctgcggcaat cgagaacctc
    10981 gacgccgccc gttccctggt cgttgaactg aagaagatgg gctgcgcggt gtcgcttgac
    11041 gatttcggta cgggttacgg ttccttcacc tacgtccggc agcttccccc ggtcgacctg
    11101 aagatcgatt cctcgttcgt gatggatctc gattcggagg aggccagccg cagggtggtg
    11161 agttcgatcg tttcgatctc gaagaccttt ggtgtctcaa ccgtggccga aggcatcgag
    11221 gacgaggcca cgctcgaact cgtcaagggc atgggaatca atttcctcca gggctttcac
    11281 ctcggtactc ctcagcccct cgaccggtag gaacggcggt ttgccgaaag cattccgggt
    11341 actgaagccg gtatggcaaa gatcggtttc accctttcca gcgaagaaca gcctcccggc
    11401 ctgctcgtgg agcacgcagc cgctgccgaa caggcgggat tcgacttcct ctccatctcc
    11461 gaccactttc acccctgggt cgaccgtcag gggcagtcgc cgttcgtctg gtcggtgctc
    11521 ggagcggtat cccgggcgac cgagcggatc gagttgatta ccggggtgac ctgcccgact
    11581 acccggatcc acccggcgat catcgcccag gcggcagcga ccaccgcctg tcttatgccc
    11641 ggacgtttct ccctcggtct cggcagcggc gagaacctga acgagcacat ccttggtgac
    11701 cgctggccat cggtcgacat ccgccaggaa cgcctcgaag aagcggtaac cgtgatccgc
    11761 tcactgtggg agggcggtct gaccaatcac cggggagtcc actacgaggt cgagaacgcc
    11821 cggatctaca cgctgcccga ccagcttcct ccgatcctga tcgcaaccgc cggcgaacag
    11881 gcgacggaac tggccggacg ggtgggggac ggactggtcg gcctgattcc cgattcatcg
    11941 gtgatcgagc agttccgcga gtccggcggg gaaggcaagc cggcctatgc ccaggtccac
    12001 atttcctggc gcgagtcgaa ggaggaagcg gtgaagacgg cccttgagtg gtggccgaac
    12061 gccggcgtgc ccggcaacct cttcgtcgag ctgccgctgc cttcgcactt ctccgaagcc
    12121 gcatcgaccg tggacgagga cctgattgcc gaatctgtcg tctgcggccc ggatcccgaa
    12181 ccgatcgtcg aggagatagg ccggtatctg gacgccggct tcagccatgt ctatgtccac
    12241 caggtcggcc ccgacactcc gggtttcttc gacttctttc gcgaccgggt tctgccggag
    12301 ttccaggcct gagtccggtc cggacctcac ccgccgcaac ggcccgtcgc ggaagccgcg
    12361 gtcgggtact tttggcacat gagccccggt cacccttcag gacgacagtt cgaaatcacg
    12421 catgggtcgc agtcagcgac ggtcgtcgaa gtcggcggcg gcatccggac ctacgaggtc
    12481 gagggacgat ccgtgctcga cccctatccg gtcgaacgaa tctgcgacgg ggcccacggg
    12541 gctcccctga tcccgtggcc caaccggctc gaccgcggcc gctacagctt cgacggcgag
    12601 gcatatcaac tgcctttgac cgaaccggaa caggacaacg cgatccacgg cttcctgcgc
    12661 tgggtcacct gggcggcgga agttcacgga cccgacgcag tgacgatgtc gaccacggtc
    12721 gcgccccggc cgggttatcc gttctggctc gatgtttcgg tcggctaccg gcttggcgac
    12781 aagggactcg aagtgtcaac cgaaatcgtc aacaagggcg agcgcgcctg cccggtcggc
    12841 cacggccagc atccctacct gtcggccggc agcggcttga tcgacgactg cctgctccag
    12901 catcccggca ccgtccggat cctcaccgat gaccgccagt tgccggccgg gagggaggag
    12961 gcgtcgggca cgaaattcga cttcgccgag ccgcgtcagc tcggggacat cgagatcgac
    13021 ttcgcgttca ccgacctgcg tcgcgacggg gaggggaagg cctggacccg gctcgagggt
    13081 gccgacggga agactgcgtc gatctgggtc gaccggaact acccgctggt ggagctctac
    13141 acgggccaca cgctcgcccc ggaccgggcc cgtaccggcc tcgggaccga gccgatgact
    13201 tgtgctccgg atgccttcaa caatggcgag gggttgatcc ggctcgagcc cggggagagc
    13261 gcgtcagccg tctggggcgc ttgcctcgag ggctgaacat ggcgcaccgg cagacattcc
    13321 tgatccttgg cgccagcggt gacctgactt cccggctgct tttgccaggg ctcgcctcgc
    13381 tcgtcgatgc cggccagact ggcgacctgc tcctggtcgg cagcagcgtg gacgagtgga
    13441 gccaggaaga gtggcgggac cgggtccgaa actcgctcga gaagtcggga ggagcggggg
    13501 aggggtccat ggcactcgcc gcctccgccc gctacgtcca ggccgacgtc accgacgtgg
    13561 aaggcgtgag aagcgttctg gccgcggtcg aaggccagcc gatcatctat ttcgcgttgc
    13621 cgcccgaggt gtcgatcacc gcctgtgagg tactgcggaa ggtcggctgt ccacctgaaa
    13681 cccggctcgt tctcgaaaag ccgtttggcc gggacgcgga atcggctgaa gccttcaacc
    13741 gtttgctggc ggagatagtt ccggagagcc aggttcaccg cgttgaccac tttctcggcc
    13801 tctccacggt gctgaacatc ttcggtctcc ggtttgcgaa ccgggtcttc gagccgatga
    13861 tgaactccca tcacgtcgag agcgtcgaga tcctctggga tgaggacctc gccctggaag
    13921 ggcgagccgg ctattacgac cgtgccggtg cgctggtcga catgatccaa agccatctgc
    13981 tccaggtggc tgcggtgctg gcgatggaac cgccaccgac gaccggcgag aaggacatcc
    14041 gtgacggcac cgccaggatc cttcgcgccg cttcggtctg ggatgacgac ccggccaggt
    14101 tcagccgccg ggcgaggtac tcggcgggga cgatcaaggg ccgcgaactt cccgcctatg
    14161 cagacgagga aggggtcgag ccggagaaga tgaccgaaac gttcgccgag atcgtgatcg
    14221 gtgtcgatac ctggaggtgg gccggggttc cctttcgcct gcgctcgggc aaggccatcg
    14281 gcaatccacg gaaagaggcg gtcatcaact tccgcgatcc ggaacagata ccgagagggc
    14341 tggtcggcgc agagggtcag aaccgtctga ggatcggtct cggcttcggc tccgacacgg
    14401 tgcggttcga catcgcggtg aacggtcccg gcgacccgaa ggaactcgac ttcgcaactc
    14461 tcgaagcgac gcttggcgcc gggtatctgt ccgagtacgg cgaggtgctg aagacggtgc
    14521 ttgacggcac cccggcactt tcggtgcgtg gcgatgcggc ggtcgactgc tggcggatca
    14581 tcgacccggt ccggcaggca tggaagacgg gcgaggtgcc cctgctcgag tacccggccg
    14641 gctcgagcgg accggagccc tggccggaga gcggccttcc gggatctcag cccggcgggt
    14701 cctgagccaa atcgaaccgg gccaggtctt cggccgtcgc ctgcccggcg tagggacccc
    14761 gcccctgcag gttcgcggcc ccgcagagcg caccgatccg ggctgcctcg agaaccggaa
    14821 gtccgagacc gaggccgtaa gtgattccgg cggcgaatga gtcacctgcc ccgtagctgt
    14881 cgacccacgg tgactcg
//
LOCUS       DAOQKN010000042        14610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_1914_length_14610_cov_5.642597, whole genome shotgun sequence.
ACCESSION   DAOQKN010000042 DAOQKN010000000
VERSION     DAOQKN010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 14610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14610
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1914_length_14610_cov_5.642597"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="PLJ64_04455"
     CDS             <1..267
                     /locus_tag="PLJ64_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92773.1"
                     /translation="AVSPYVAGKVIKGPTDLFMKAIGRPTTATGVAGLYQGLINAMIT
                     DADDPDPPPTEVRSFSTATLMHSAESRATVARAVIKVGETLAGS"
     gene            264..917
                     /gene="cofC"
                     /locus_tag="PLJ64_04460"
     CDS             264..917
                     /gene="cofC"
                     /locus_tag="PLJ64_04460"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HNC92774.1"
                     /translation="MNSVAVITAKRFPAAKQRMAGGVPEERRLALVEAMLGDVLEAVT
                     GARQVNFTVVVTGEVAAADLAAGHGAEVIHDPEDAGHIGAAELGIERAIELGAECVIL
                     LPGDCPLLEAREIDSLLTGIPEKFVTVVPDRHGTGTNALVLRPPDAIKPAFGEGSCQR
                     HLRLARDAGLPHDTEEVAGLALDLDTPADIVALTTKIEMVRATGGNPAPRTAAVLGI"
     gene            918..1670
                     /gene="cofE"
                     /locus_tag="PLJ64_04465"
     CDS             918..1670
                     /gene="cofE"
                     /locus_tag="PLJ64_04465"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HNC92775.1"
                     /translation="MDAAMIIPVEGLPEVTETFNIGKAIAERVQMTDGDIVAVSQKIV
                     SKSEGRVVRLDETDPGPDAVSLAARTGKDPRLVQHVLNESRNLIRVDEQRSVLITETH
                     HGYICANAGIDQSNTAGENQAILLPLDPDGSARRIRVELETASGVKAAVVVTDSFGRA
                     WRHGQVETAIGCAGIDPLDDWRGQEDQRGRELTATVIAVADQIAAASDLVRTKTDGVP
                     AVVLRGLDHFVTEDDGPGCAAQLREPGDDLFR"
     gene            1684..2250
                     /locus_tag="PLJ64_04470"
     CDS             1684..2250
                     /locus_tag="PLJ64_04470"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNC92776.1"
                     /translation="MGRLIYGSMSITLDGYFADADGGIGWTVPEDEVHRLANQQVRGH
                     AAVIMGRGLYEMMVPYWYDLLGSGEGGEIEQEFAMVWDEIPKFVASRTLTAVHETCQL
                     IEGDAVDWIARKKAETDGDIEVSGIDLASQVEAAGLIDVYRFNLLPVVLGGGKPFFPG
                     NGTRRDLELTDLQRFPGGTVALEYTVKR"
     gene            complement(2240..3223)
                     /locus_tag="PLJ64_04475"
     CDS             complement(2240..3223)
                     /locus_tag="PLJ64_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HNC92777.1"
                     /translation="MRAMVLESQPGDLVPRELPVPEPGPGQILIRVSTCGVCRTDLHI
                     VDGELSEPKLPLVPGHQIVGTVEAAGEDADRFGTGDRIGVPWLGWTDGDCRFCREGRE
                     NLCENARFTGYDSDGGYAEFAVADQRFCFPLSAAIDDTHVAPLLCAGMIGYRALAIAG
                     DGERLGLYGFGSSAHILCQITAAEGRRVFAFTRPGDQAGQAFARSLGAEWAGGSDETP
                     PEPFDAAIVFAPVGSLMVDALRASERGARIVSAGIHMSDIPSFPYELLWEERSLHSVA
                     NLTRADGTALLELAARHRVEPMVELLPLESANQALDGIRSGASEGSTVLTV"
     gene            complement(3242..5593)
                     /gene="cofH"
                     /locus_tag="PLJ64_04480"
     CDS             complement(3242..5593)
                     /gene="cofH"
                     /locus_tag="PLJ64_04480"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HNC92778.1"
                     /translation="MRRVTFSRNYTISLSRTCQCYCKYCAFATHQAHVHPPGEVERML
                     DEAVKRRAKELLFLTGEKPEVNPAVKEQLRSWGHDDFTSYVIWACERAMERGILPHTN
                     IGAIGKEDLARLREVTASQGLMLESSSERLMKTVHAGSPTKHPAHRLKTIEAAGQLKI
                     PFTTGILVGIGETPEERIESLEALAALQQKYGHIQEVIIQNFVPHPRYYGQEPADIAA
                     EASDRRWKESGTVVSLDSGIEAGAGEETANHDPGRPTIPLPDWACPVSIGDLKHLIRE
                     TARLMPGVGVQVPPNLSDWWAELVEAGATDLGGLSANGDHISPEHPFPSPHQVRKELQ
                     PMGYALTERLCVYPQYMNADWMEQGVLDVIKLKYWSFIPRLGSGRVSEETIDPGLAIE
                     AIAKGREGEELTEDELTALFAETRPEVIEAMRVAADELRAELAGNTATFVVNRNINFT
                     NICTVGCAFCGFGQGRRSPDAYESTEEEFIGRVEEAVEFGATELCMQGGIHPELDLEA
                     YGSWLKLAKQVAPDIHLHAYSPMEINHMCEVSGLPPSEVFEYLIECGLGSTPGTAAEV
                     LDDGVRQRISPNKLPVARWVEIIEASHRAGLKSTSTVMFGHIEEPRELARHMKVIRDL
                     QGRTGGITEFVPLSFIPFNTLLGRTHGVEEISIDENLKHTAAFRLALGKSVTNLQASW
                     VKMGLPGATEALNWGVNDLGGTLMEESISRMAGSQHGVRLEVEELVDAAHRAGRRAAQ
                     RNTSYEIIREFPDSVTSPPPEMLIDLAWPGEEPAPEIADPAAA"
     gene            5696..6133
                     /locus_tag="PLJ64_04485"
     CDS             5696..6133
                     /locus_tag="PLJ64_04485"
                     /inference="COORDINATES: protein motif:HMM:NF012355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HNC92779.1"
                     /translation="MKASWKYLVVPLAVGALIFAGCGSDDSSDDSGSSDTTTTQSQPA
                     DNSAMGGQVLKLAADPSGALAYDTTELSAKSGKVTLDFTNDSPVGHDVVIEDSNGKEV
                     GSTDVITGQSTTAEFTVKPGTYTFYCSLPGHEEAGMKGTLTVK"
     gene            6158..6559
                     /gene="crcB"
                     /locus_tag="PLJ64_04490"
     CDS             6158..6559
                     /gene="crcB"
                     /locus_tag="PLJ64_04490"
                     /inference="COORDINATES: protein motif:HMM:NF010812.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HNC92780.1"
                     /translation="MFQRHPLRKLTAIYVGGVVGALLRVGLAEAFPHAVDAWPWPTFA
                     VNLAGAFLIGYFFTALAGQAPDRLHHPFLATGICGTLTTFSTLQLEIFNMVDGGEPGL
                     AFAYAALTVALGYLAVRLGIVAARPGPGVRP"
     gene            6571..6927
                     /gene="crcB"
                     /locus_tag="PLJ64_04495"
     CDS             6571..6927
                     /gene="crcB"
                     /locus_tag="PLJ64_04495"
                     /inference="COORDINATES: protein motif:HMM:NF014584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HNC92781.1"
                     /translation="MSLFGWAAVAGLGGLAAGARYLLDAEISRRSDNPFPVGILVVNL
                     LGSFLLGLVAGSTIHGQALVIVAGGVIGSFTTFSTWMLDTHLLASADLAKLAWLNVAI
                     SLALGLAAVTLGQAIT"
     gene            complement(7018..7770)
                     /locus_tag="PLJ64_04500"
     CDS             complement(7018..7770)
                     /locus_tag="PLJ64_04500"
                     /inference="COORDINATES: protein motif:HMM:NF012455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HNC92782.1"
                     /translation="MQDRGLAAYIAELLGTFFLVFGITLVGVLFIAVGDNASFGSDFA
                     VVGLTYAFILTALIFAFGGPSGGHFNPAITLGAALMKKIAPVDAVIYILAQLSGGVLG
                     ALLTKGLLEDEGRAANYGAVSISPLLSGNFAGGVVECLGTFLLVLAVLSVALGVRAVK
                     DWAPLAIGLTLGVVGLILVPLTGGAVNPARWFGPALVSGEWGGVWPYLGGEIVGAVLA
                     FVVYKFVIAPGVDDGSRSEPAKKPSTIPSPEA"
     gene            complement(7952..8272)
                     /locus_tag="PLJ64_04505"
     CDS             complement(7952..8272)
                     /locus_tag="PLJ64_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92783.1"
                     /translation="MARVLALVPDLMLSSRVSGMLGAAGHEVTVAPTPPVAADFDFDL
                     IVCDLEACDPAAVAKLPAPAIGFYSHVDVETRDRGREAGLDLVVPRSRMARELPGLAA
                     RLLD"
     gene            complement(8293..8655)
                     /locus_tag="PLJ64_04510"
     CDS             complement(8293..8655)
                     /locus_tag="PLJ64_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92784.1"
                     /translation="MALAQDFQDVLDSLPADWTDLEFDLRIDDEDRYIDASVHLSLIN
                     AQPYSQAEWHWRVPVAHSFGKAAAPQTVLGVLRRLDKEGFTGELNLREVREGRSEVVQ
                     MWGRPESVREEFRQRRSI"
     gene            8737..9705
                     /locus_tag="PLJ64_04515"
     CDS             8737..9705
                     /locus_tag="PLJ64_04515"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HNC92785.1"
                     /translation="MSPVTEPDLDAEYRQIREECGLIERADRAWLEVTGPDAAGFLQS
                     QVTNETEELAAGSGVYAALLDRKGHLQAEMRILRLDGERFLIDTEATTGPALLKHFSM
                     YKIGRKVEVGETDRALFSLIGPAAVEVTGLTPGSENDFTETVIAGAGCLVVVTALGLD
                     VICDPGSSDAVRAQLEADRAVPVSAEAAEILRVERGRPRFGFDMTEANMPAEAGIVDR
                     AVSFTKGCYIGQEPVARLHYKGRPNRHLRGLRPGGPVAHGDPVRLGDRQLGEVGTAVL
                     SPASGRIALSILRKEAEPGSVVTVETEAGEVEAEVVELPFIEGSGI"
     gene            9702..10751
                     /locus_tag="PLJ64_04520"
     CDS             9702..10751
                     /locus_tag="PLJ64_04520"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNC92786.1"
                     /translation="MSEVRRQGLGGGKLGADSPLFGGSGASTNGDGDPAGDRFREPDE
                     QPAWDPVPDDFAESREAAAAEANRAAEELLKLGDLRDLLPAAEPDRALDDWGRSETVV
                     SLMEPLLNFYYRYWFRVEVEGLENVPSEGGALIVSNHSGALPPDAPMIMQAIRNEHPA
                     PRPLYMLGEHWFKGYPGVSMLVNKMGLVPAHRENAQRLLHDEGRLAVVFPEGQKGTRK
                     VYWQRYKLRRFGRGGFVRTAIRARVPIVPVAVIGAEESAPIFAHFKLLQKLSGLIYFP
                     LTPSFPHFGLLAPLMYLPSKFKIRFMEPIDMAEFPVETIDDPAEIQVISERIRARIQH
                     QLEEMLDARESVWTG"
     gene            10780..11256
                     /locus_tag="PLJ64_04525"
     CDS             10780..11256
                     /locus_tag="PLJ64_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92787.1"
                     /translation="MRGIRVTAFALLAATAAIAGCGSSTHENEPRPPVPTVVSISVGK
                     DTIDAKAAIGRGVGEPGPRQPYLNQNRTAPQNQADRKAPAVVNFAIANLTDRATVLRM
                     QGEVSRTVALTPGGSGSFMMALPTGIYRFSSPASSDTALLNVGPSRVSSGGDLLLP"
     gene            11415..12674
                     /gene="ccrA"
                     /locus_tag="PLJ64_04530"
     CDS             11415..12674
                     /gene="ccrA"
                     /locus_tag="PLJ64_04530"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HNC92788.1"
                     /translation="MSVDLADVSKLDVQPGELPETMAAWVIRPETEGNEPNKAFQFED
                     IETPEPGAFEVIVRVMAAGVNFNNVWAGLAEPVSVFPYGDHPEYGHHIGGSDASGIVW
                     KVGEGVTRWKVGDEVVVHCNQASYEDVEVHGLDPMAAPSQKIWGYETTWGSFAQFTKV
                     QAQQLLPRPKNLSWAEASSYGLTYFTAYRMLMDQARIQAGSNVLIWGAAGGLGVFAVQ
                     LCKAAGANAVGVVSSDEKGELVKQLGAVDYINRNEFTDMMRTGNETPEEEKARFKASV
                     GFAKKVKSILGDSPDVVFEHVGQATFPTSVFVVKPFGKVVICGATSGYTLDFDVRHLW
                     MRQKQIIGSHFANAYECMKANQLMAEGKVQPVLWKTMGFEGVPEAHQMLHENRHLGKI
                     AILVGATSEDEGREEEGPGAIRAEVGA"
     gene            12705..13001
                     /locus_tag="PLJ64_04535"
     CDS             12705..13001
                     /locus_tag="PLJ64_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92789.1"
                     /translation="MTYISEISWHVTPFRSQRWIDTWEPAAAKATEYGATEWEIYRSD
                     EDPLLFRQVTRWEKKSDFEAWWYSEEISAIRAEVVDLYDKPLLPVWCSRVIASS"
     assembly_gap    13290..13389
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(13549..14253)
                     /locus_tag="PLJ64_04540"
     CDS             complement(13549..14253)
                     /locus_tag="PLJ64_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92790.1"
                     /translation="MRSLTKNPLLRAIVAAVSLGAVLFAANAAASTITIYKNDLDTTD
                     SRAEIHQMAPRANCERGGSPNAFRFQLGKKARECFYKVPMAGKNLQVTAIARIFKSTP
                     PKVLRRAYAGVSLRQNNDGSRYQLVVWPSTRRYGLRKVQPNGQIINFGSRKAGRKVKG
                     PGKANRLTLRAFTGQKGNTQLVGFVNGHKVAAARDDQGTSLVGQDTSISIGAKQSARG
                     AIGSFADIRVAIPDPY"
BASE COUNT         2495 a         4835 c         4807 g         2373 t
ORIGIN      
        1 gcggtcagcc cctatgtggc cggcaaggtg atcaagggcc cgaccgacct cttcatgaag
       61 gcgattggcc ggccaaccac ggcgaccgga gtggccggtc tctatcaggg tctgatcaac
      121 gcgatgatca ccgacgccga tgatccggac ccgccgccga ccgaggtccg ttccttctcc
      181 acggcgaccc tgatgcacag cgccgagagc cgggcaacgg tcgcccgggc cgtgatcaag
      241 gtcggcgaga ccctggccgg atcttgaact cggtcgcggt aatcacggca aagagatttc
      301 ccgcggccaa acagcgcatg gccggcggag tgcccgaaga gaggcgcctg gccctggtcg
      361 aggcgatgct cggcgacgtg ctcgaggcag tgaccggggc aaggcaggtc aacttcacgg
      421 tagtcgtcac cggcgaggtt gccgcggcag acctggcggc cggccatggc gccgaggtga
      481 tccacgaccc ggaggacgcc ggtcacatcg gtgccgccga actcgggatc gagcgggcga
      541 tcgaactcgg agccgagtgc gtgatcctcc tgccgggcga ttgtccgctg cttgaggccc
      601 gtgaaatcga cagcctgctg accggcatcc cggagaaatt cgtcacggtt gttccagatc
      661 ggcacggaac gggtacgaac gcccttgtgc tccgtcctcc tgatgcgatc aaaccggctt
      721 tcggcgaagg aagctgccag agacacctcc gcctcgcccg ggatgcgggg ctgccgcacg
      781 acacggaaga ggttgcggga ctggccctgg atctcgatac tccagctgac atcgttgccc
      841 tgaccacaaa gatcgagatg gttcgtgcca ccgggggaaa tccggccccg cggaccgccg
      901 ccgtcctggg catctagatg gacgccgcga tgatcatccc ggtcgagggt ctgccagagg
      961 tgaccgaaac cttcaacatc ggcaaggcga ttgccgagcg ggttcagatg accgacggcg
     1021 acatcgtcgc ggtttcgcag aagatcgtct ccaagtcgga aggtcgcgtc gtcaggttgg
     1081 atgagaccga ccccggaccg gatgccgtct cgctggccgc ccggaccggc aaggatccga
     1141 ggctggtgca gcacgtgctg aacgagagcc ggaacctgat ccgggttgac gagcagcgga
     1201 gcgtcctgat caccgagacc catcacggct acatctgcgc aaacgccggc atcgaccagt
     1261 cgaatacggc cggcgagaac caagcgatcc tgctgccgct ggacccggac ggttcggccc
     1321 ggcggatccg ggttgagctg gaaaccgcat cgggggtcaa ggcggcggtg gtggtcaccg
     1381 acagcttcgg ccgggcctgg cgtcacggcc aggtcgagac cgcgatcggc tgcgccggaa
     1441 tcgacccgct ggacgactgg cgcggccagg aggaccagcg gggccgggag ctgaccgcaa
     1501 ccgtaatcgc ggtggccgac cagatcgccg ccgcttccga cctggtccgg accaagaccg
     1561 acggcgtgcc agccgtggtc ctgagagggc tcgaccattt cgtcacggaa gacgacggcc
     1621 cgggctgcgc ggcccagctg cgcgaacccg gtgacgacct cttccgctag ttaccctgag
     1681 tccatgggac gactcattta cggatcgatg tcgatcacgc tggatggcta ctttgctgac
     1741 gccgacggcg gtatcggctg gaccgtgccg gaggatgaag tccaccggct cgccaaccag
     1801 caggttcgag gtcacgcggc cgtcatcatg ggccgcggcc tctacgagat gatggtcccg
     1861 tactggtacg acctcctggg gtccggcgag ggcggtgaaa tcgagcagga gttcgccatg
     1921 gtctgggacg agataccgaa gttcgttgcc tcgcgcaccc tgaccgcggt tcacgagacc
     1981 tgccagctga tcgagggcga cgcggtggac tggattgccc gcaagaaggc cgagaccgac
     2041 ggcgacatcg aggtgagcgg catcgacctc gcctcccagg tcgaggcagc cgggctgatc
     2101 gacgtctacc gcttcaatct cctgcccgtc gtcctcggcg gcggcaagcc gttcttcccc
     2161 gggaacggaa cccggcggga ccttgaactg accgatctgc agcgcttccc gggcggcacc
     2221 gtcgcgctcg agtacaccgt caaacggtga gtacggtgga accttccgag gcgccgctcc
     2281 ggatcccgtc cagcgcctga ttcgcacttt cgaggggaag cagctcgacc atcggctcga
     2341 cccggtgccg ggccgcgagc tccagcaggg cggtgccgtc ggcccgggtc aggttcgcca
     2401 ccgaatgaag gctccgctcc tcccagagca gctcgtaggg gaacgagggg atgtcgctca
     2461 tgtggatgcc ggcgctgacg atcctcgccc cgcgctcgct ggcccggagg gcatcgacca
     2521 tcagcgaacc gaccggggcg aagacgatcg ccgcgtcgaa cggctccggc ggagtctcgt
     2581 ccgacccccc ggcccactcg gccccgagcg agcgggcaaa tgcctggccg gcctggtcac
     2641 cgggtctggt gaaagcgaaa acgcggcggc cctcggcggc cgtgatctgg cagaggatgt
     2701 gagcggagga tccgaaaccg tagagaccca gtcgctcgcc gtctccggcg atcgccagcg
     2761 cccggtagcc gatcatcccg gcgcaaagga gcggtgccac gtgagtgtca tcgatcgccg
     2821 cggaaagggg gaaacagaac ctctggtcgg cgaccgcgaa ctccgcgtac ccgccgtcgg
     2881 agtcataacc ggtgaagcgg gcgttctcac agaggttctc ccggccctcc cggcagaagc
     2941 gacagtcccc gtcggtccag cccagccagg gcacgccgat ccggtcaccg gtcccgaagc
     3001 ggtccgcgtc ttctcccgcg gcctcgaccg tgccgacgat ctggtggccg ggaaccagcg
     3061 gcagcttcgg ctcgctcagc tcgccgtcaa cgatgtgcag gtcggtccgg caaaccccgc
     3121 aggttgaaac ccggatcaga atctggccgg gaccgggctc ggggacggga agttcccgcg
     3181 ggacgaggtc gccgggctgg ctttcgagga ccatcgcgcg catcgcccgg cccctcccga
     3241 ttcaggccgc ggcgggatcc gcgatttcgg gggccggttc ctcccccggc caggccaggt
     3301 cgatcagcat ctcgggcggc ggggaggtca ccgaatcggg gaactcgcgg atgatttcgt
     3361 aagaggtatt ccgctgggcg gcccggcgac cggcacgatg ggcggcgtcg accagttcct
     3421 cgacctcgag ccggactccg tgctgggaac cggccatacg ggagatgctc tcctccatca
     3481 gggtgccgcc gagatcgttc acaccccagt tgagcgcttc agtcgctccc ggcaggccca
     3541 ttttcaccca gctcgcctgg aggttcgtga ccgacttgcc gagcgcgagg cggaaggccg
     3601 cggtgtgctt gaggttctcg tcgatcgaga tctcctccac cccgtgagtt cgcccgagca
     3661 gcgtgttgaa cggaatgaag ctcagcggca cgaattcggt gatgccgccg gtgcggccct
     3721 gaaggtcacg gatcaccttc atgtgacggg caagttcgcg cggttcctcg atgtgaccga
     3781 acatcaccgt cgaggtcgac ttcagcccgg cccggtgtga agcctcgatg atctcgaccc
     3841 agcgggccac cggcagcttg ttcggagaaa tgcgctggcg cactccgtcg tcgagcacct
     3901 cggccgcggt gccgggggtc gaacccagac cgcattcgat caggtattcg aagacctcag
     3961 acggcggcag acccgagacc tcgcacatgt ggttgatctc catcggcgag taggcgtgga
     4021 ggtggatgtc gggcgcaacc tgcttggcga gcttcagcca gctcccgtag gcctcgaggt
     4081 ccagttcggg gtggattccg ccctgcatgc agagctcggt cgcgccgaac tcgaccgcct
     4141 cctcgacccg gccgatgaac tcctcctcgg tcgactcgta ggcgtcgggc gagcggcgac
     4201 cctggccgaa gccgcagaag gcgcagccga ccgtgcagat gttggtgaag ttgatgttgc
     4261 ggttgaccac gaaggtggcc gtgttgccgg ccagttcggc ccgcagttcg tcggcggcga
     4321 cccgcatcgc ctcgattact tcgggccggg tctcggcgaa gagcgcggtc agctcgtcct
     4381 cggtcagttc ttccccctcg cggcccttgg cgatcgcctc gatcgccagc ccggggtcga
     4441 tcgtctcctc gctgacccgg ccggagccga gccgcggaat gaagctccag tacttgagct
     4501 tgatcacgtc gaggacgccc tgttccatcc aatcggcgtt catgtattgc gggtagacgc
     4561 agagccgctc ggtcagggcg taacccatcg gctgcagctc cttccggacc tggtgggggc
     4621 tcgggaaagg gtgctcgggc gagatgtggt caccgttggc ggagagcccc ccgaggtcgg
     4681 ttgccccggc ctcgaccagc tccgcccacc agtcggaaag gttcggtggc acctggaccc
     4741 cgacccccgg catcagccgg gccgtttcgc ggatcaggtg cttcaggtcc ccgatcgata
     4801 ccgggcaggc ccagtccggc agcgggatgg tcgggcgtcc ggggtcgtgg tttgccgttt
     4861 cttcgccggc cccggcttcg atgccggaat cgaggctcac gaccgtcccc gattccttcc
     4921 agcgccggtc ggaagcttcg gccgcgatgt cagccggttc ctgcccgtag tagcggggat
     4981 gcggcacgaa gttctggatg atcacttcct ggatgtggcc gtacttctgc tgcagggcgg
     5041 ccagcgcctc aagcgactcg atccgttctt ccggggtctc gccgattccg accaggatgc
     5101 cggtggtgaa gggaatcttc agctggccgg ccgcttcgat cgtcttcagg cgatgagccg
     5161 gatgtttggt cggcgagccg gcgtgaacgg tcttcatcag gcgctcggaa gacgattcga
     5221 gcatcaggcc ctgggaggcg gtgacctcgc gcagtcgtgc caggtcctcc ttgccgattg
     5281 cgccgatgtt ggtgtgggga aggatccccc gctccatcgc ccgttcgcag gcccagatca
     5341 catatgaggt gaagtcatcg tgcccccagg agcgcagctg ctccttcacg gccgggttga
     5401 cttccggctt ctcgccggtc aggaagagca gttccttggc ccgccgcttg accgcctcgt
     5461 ccagcattcg ctcgacttcg ccgggcgggt gaacatgggc ctgatgcgta gcgaaggcgc
     5521 agtacttgca gtagcactgg caggtccgcg aaagcgagat cgtgtaattg cgcgaaaagg
     5581 tgacccgacg catccggctt ccaacctacc gcagtgaacc ttccgcttcg gcccggcgta
     5641 gcacccgggt agtgtgaatg cacttcccgt ccatcgaccc cccaaggaga acgcgatgaa
     5701 agcttcatgg aagtatctgg tcgtaccgct tgcggttgga gccctgatct tcgccggctg
     5761 cggtagcgac gattcatccg acgacagcgg atccagcgac accaccacaa cccagagcca
     5821 gcccgccgac aacagtgcca tgggcggaca ggttctgaag ctggctgccg atccgtcggg
     5881 tgccctcgct tatgacacga ccgagctgtc cgccaagtca ggcaaggtca cgctcgactt
     5941 cacgaatgat tcgccggtcg gccacgacgt ggtgatcgag gactcgaacg gcaaggaagt
     6001 cggttcgacc gacgtgatca ccgggcagag cacgacggcc gagttcacgg tcaagcctgg
     6061 cacctacacc ttctactgct cgctgccggg ccacgaggaa gcgggaatga agggaacgct
     6121 caccgtcaag tagacgaagg ggtaccttcc tgccctgatg tttcagcgcc acccacttag
     6181 aaagctcact gcgatctatg tgggtggcgt cgtcggggcc ctcttgaggg tcggcctcgc
     6241 cgaagccttt ccccacgcgg tcgatgcctg gccctggccc acctttgcgg tcaacctggc
     6301 cggggcattc ctgatcggtt acttcttcac cgcgctcgcc ggtcaggcgc ccgaccggct
     6361 gcaccaccct ttcctggcaa ccgggatctg cggaaccctg accaccttct cgaccctcca
     6421 gctcgaaatc ttcaacatgg tcgacggcgg ggagcccggg ctggccttcg cctacgcggc
     6481 cctcaccgtt gccctcggct acctcgcagt gaggctgggc atcgtggccg ccagaccagg
     6541 gccgggggtt cggccttgac cgtctttggt gtgagcctgt tcggctgggc tgcggtcgcc
     6601 gggttgggcg ggcttgcggc cggcgcccgc tacctgctcg acgccgaaat ctcccgccgc
     6661 tcggacaacc ccttcccggt cgggatcctc gtggtcaacc tgctgggctc cttcctgctc
     6721 ggcctcgtcg ccggcagcac aattcacggc caggccctgg tcatcgtcgc cggcggcgtg
     6781 atcggctcgt tcaccacctt ctcgacctgg atgctcgaca cccatctcct cgcctcggcc
     6841 gacctggcga agctggcctg gctgaacgtt gcgatcagcc tggccctcgg cctcgccgcg
     6901 gtcacgctcg gccaggcgat cacctgagcc gggccgcccc cgacgacgaa gggcggcccg
     6961 gaggccgccc tttcgaaact gctcgagttt tgagcccggc gtgccgggct tgccgctcta
     7021 ggcctccggc gacgggatcg tcgagggctt cttggccggt tcggaccggg atccgtcgtc
     7081 gacgccaggc gcgatcacga acttgtagac cacgaaggcg aggacagcac cgacgatttc
     7141 accgccgagg tagggccaga caccgcccca ttcgcccgaa acgagggcgg ggccgaacca
     7201 gcgggccggg ttgacggcgc caccggtcag cggaaccagg atcaggccga cgacaccgag
     7261 cgtgagtccg atcgccagcg gagcccagtc cttgaccgca cggacgccga gggcaaccga
     7321 aagcacagcc agaaccagca ggaaggtacc gaggcactcg accacgccgc cggcgaagtt
     7381 gccgctcagc agcgggctga tcgacaccgc accgtagttg gcggcgcgcc cttcgtcttc
     7441 cagcagaccc ttggtcagca gagcgccgag gacaccaccg gagagctggg cgaggatgta
     7501 gatcactgcg tcaaccgggg cgatcttctt catcagcgcc gcaccgagcg tgatcgccgg
     7561 gttgaagtga ccaccgctgg gcccgccgaa ggcgaatatc agcgccgtga ggatgaaggc
     7621 gtaggtcaga ccgacgaccg cgaagtcaga accgaatgag gcgttatcgc ccacggcaat
     7681 gaaaagcacg ccgaccagag tgattccgaa caccaggaag aacgttccca gcagttcggc
     7741 gatatatgcg gcgagaccgc gatcttgcat gaagtcaaac ctcccgtggc ttgtatcgat
     7801 tgcgccccac cgtttgcggc gagccgtggc agtctaacca gagcccgttg ccgaacggtt
     7861 ggaagcgcaa tacggggtcg catgtttggg cctagaaata tacgccgaca aaggcccggt
     7921 tcctgccgcg ggagggtcgg ggtgagggtt ttcagtccag cagccgggcc gcgaggccgg
     7981 gcagttcccg ggccatgcgg gaccggggaa cgaccaggtc gagaccggct tcccggcccc
     8041 ggtcgcgggt ctcgacgtcg acatgcgagt agaaaccgat cgcgggcgca ggcagctttg
     8101 ccaccgccgc ggggtcacag gcttcgagat cacagacgat caggtcgaag tcgaaatcgg
     8161 cggccaccgg aggggttggc gcaaccgtca cctcatggcc ggcggcaccg agcatcccgc
     8221 tgacccggct cgaaagcatc aggtcgggaa caagggcgag cacgcgggcc attgcggttg
     8281 ccggaccggg cctcagatcg agcggcgctg acggaactct tcgcgcacgc tctccggccg
     8341 gccccacatc tgaactacct cggagcggcc ttcacggacc tcgcgcaggt tgagctcacc
     8401 ggtgaacccc tccttgtcga gccgccgcag caccccgagc accgtctgcg gagccgccgc
     8461 cttcccgaag ctgtgggcga ccggaacccg ccagtgccac tccgcctgcg aataaggctg
     8521 ggcgttgatc aggctgaggt ggacggaagc gtcgatgtat cggtcttcgt catcgatccg
     8581 cagatcgaac tcgaggtcgg tccagtcggc cggcagagaa tcgagcacat cctgaaagtc
     8641 ctgggcgagt gccatcaggg cgaagcctag cgcggcccgg gtccggctcg cccccgggat
     8701 gacccggcgg gagttggccc gtcgtagaat tggcccatgt ccccggtgac cgaacccgac
     8761 cttgacgccg agtaccggca gatccgcgag gaatgcggcc tgatcgaacg ggctgaccgg
     8821 gcctggcttg aagtcaccgg gccggacgca gccgggttcc tgcagagcca ggtgaccaac
     8881 gagaccgaag aactggcagc cggatccggg gtatatgccg ctctgctcga ccgcaaaggc
     8941 caccttcagg ccgaaatgcg aatcctgcgg cttgacggcg aacgcttttt gatcgacacc
     9001 gaggcaacca ccggccctgc cctgctgaag cacttctcga tgtacaagat cggccgcaag
     9061 gtcgaggtcg gggagaccga tcgggccctc ttctcgctga tcgggccggc cgcggtcgag
     9121 gtgaccggcc tcacgccggg aagtgaaaac gacttcaccg agaccgtgat cgccggtgcc
     9181 ggctgcctgg tcgtggttac cgcgctcggg ctggacgtga tctgcgatcc gggctcctcg
     9241 gatgcggtcc gagcccagct cgaagctgac cgggcggttc cggtttccgc cgaagcggcc
     9301 gagatcctcc gggtcgagcg gggccggccc cgcttcgggt tcgacatgac cgaagccaac
     9361 atgccggccg aagccgggat cgtcgaccgt gcggtcagct tcaccaaggg ctgctacatc
     9421 ggccaggaac cggttgcccg gcttcactac aagggccggc cgaaccgtca cctgcggggg
     9481 ctgaggcccg gcggaccggt cgcccacggg gatccggtcc ggctcggcga tcgtcaactc
     9541 ggagaagtcg gcaccgcggt cctctccccc gcatcgggcc gaatcgcgct ttccatcctt
     9601 cgcaaggagg ccgagcccgg ctcggtggtc acggtcgaga cggaagccgg cgaggtcgaa
     9661 gccgaagtcg tcgagttacc cttcatcgaa gggtccggga tatgagcgag gtgcgccggc
     9721 agggcctggg cggcggcaag ctcggtgccg atagccccct cttcggcgga tccggcgctt
     9781 cgacgaacgg tgatggcgac ccggccggcg accggttccg tgaacccgat gagcagcccg
     9841 cctgggatcc ggttcccgac gatttcgctg aatcacgcga agcggcagcc gccgaggcga
     9901 accgcgccgc cgaggagctg ctcaagctcg gcgacctgag ggatctgctc ccggccgccg
     9961 agcccgaccg ggccctcgat gactggggcc gctccgaaac ggtcgtgtcc ctgatggaac
    10021 cgctgctcaa cttctactac cggtactggt tccgggtcga ggtggaaggc ctcgagaacg
    10081 tcccttccga aggcggcgcc ctgatcgtct cgaaccactc cggagccctg cctccggatg
    10141 ccccgatgat catgcaggcg atccgcaacg aacatccggc cccgcgtccg ctttacatgc
    10201 tcggcgagca ttggttcaag ggctacccgg gcgtctcgat gctggtcaac aagatgggcc
    10261 tggttcccgc ccatcgcgaa aacgcgcagc ggctgctcca cgacgagggc cgcctcgcgg
    10321 tcgtattccc ggaggggcag aaggggaccc gcaaggtcta ctggcagcgc tacaagcttc
    10381 gccgtttcgg tcgcggcggg ttcgtgcgga ccgcgattcg ggcccgggtg ccgatcgtgc
    10441 cggtggccgt gatcggcgcc gaggaatcag cgccgatatt cgcccacttc aagcttcttc
    10501 agaagctctc cgggctgatc tacttcccgc tcaccccttc gttcccgcat ttcggcctgc
    10561 tcgcgccatt gatgtacctg ccctcgaagt tcaagatccg tttcatggag ccgatcgaca
    10621 tggccgaatt cccggtcgag acgatcgacg atccggccga gatccaggtg atcagcgaac
    10681 ggatcagggc ccgcatccag catcagctgg aagagatgct cgacgcgcgc gaatcggtct
    10741 ggaccggttg acgccacaca gtcaggtagt tttcaggctg tgaggggaat ccgggtcaca
    10801 gccttcgccc tgctggctgc aactgccgcg atcgccggct gcggctcatc gacccacgag
    10861 aatgaaccgc gcccgccggt tccaaccgtg gtcagcattt cggtcggcaa ggacacgatc
    10921 gacgccaagg cggccatcgg ccgcggagtc ggcgagccgg ggccccgcca gccgtacctc
    10981 aaccagaacc ggaccgcccc ccagaaccag gccgatcgca aggccccggc cgtcgtgaac
    11041 ttcgcgatcg cgaacctgac cgaccgggcc acagttcttc gcatgcaggg cgaggtcagc
    11101 cggaccgtgg ccctgacccc gggcggctcc ggctctttca tgatggccct gccgaccggc
    11161 atctaccggt tcagttcgcc ggccagcagc gacacggccc ttctgaacgt gggccccagc
    11221 cgggtctcct ccggcgggga cctgctcctt ccctgatctc acggcagtcg ggccggtctt
    11281 cgccggccct cccattgaaa cccgccgtcc caccgaagca aagaatggat gggacggctc
    11341 cgcttgaata ctgcccgggt aggtaccatt cggcggaact tccacgtccc accgaaaccc
    11401 ccgagaggaa cgaagtgtct gtagacctcg ctgatgtgag caaacttgac gtgcagccgg
    11461 gagagctgcc cgagacgatg gccgcctggg tcatccgccc cgagaccgag ggcaacgagc
    11521 cgaacaaggc cttccagttc gaagacatcg agacccccga gcccggcgct ttcgaggtca
    11581 tcgtccgcgt gatggcggcc ggcgtcaact tcaacaacgt ctgggccggt ctcgccgagc
    11641 ccgtatccgt cttcccctac ggcgaccacc cggagtacgg ccaccacatc ggtggctccg
    11701 acgcttccgg catcgtctgg aaggtcggcg agggcgttac ccgctggaag gtcggcgacg
    11761 aggtcgtcgt ccactgcaac caggcctcct acgaggacgt cgaggtccac gggctcgacc
    11821 cgatggccgc ccccagccag aagatctggg gctacgagac gacctgggga tccttcgccc
    11881 agttcaccaa ggtccaggcc cagcagctgc tgccgcgccc gaagaacctt tcctgggccg
    11941 aggccagctc ctacggtctc acctacttca ccgcctaccg gatgctgatg gaccaggccc
    12001 ggatccaggc cggcagcaac gtcctgatct ggggcgcggc cggcggtctc ggcgttttcg
    12061 ccgtccagct ctgcaaggcg gccggcgcca acgcggtcgg tgtcgtctct tccgacgaga
    12121 agggcgaact ggtcaagcag ctcggcgcgg tcgactacat caaccgcaac gagttcacgg
    12181 acatgatgcg gaccggcaac gagactccgg aggaggagaa ggcccgcttc aaggcatcgg
    12241 tcggcttcgc caagaaggtc aagtcgatcc tcggtgattc gcctgacgtg gtcttcgagc
    12301 atgtcggcca ggccaccttc ccgacctcgg tcttcgtcgt caagccgttc ggcaaggtcg
    12361 tgatctgcgg cgccacctcg ggctacacgc tcgacttcga cgtgcgtcac ctgtggatgc
    12421 gccagaagca gatcatcggc tcgcacttcg ccaacgcgta cgagtgcatg aaggccaacc
    12481 agctgatggc cgagggcaag gtccagccgg tgctgtggaa gacgatgggc ttcgagggtg
    12541 tgcccgaggc gcaccagatg ctccacgaga accgccacct cggcaagatc gccatcctgg
    12601 tcggcgccac ctccgaggac gagggccgcg aagaagaagg tccgggcgcg atccgggccg
    12661 aggtcggggc ctagacctca aaccaacggg aagaaagaag accgatgacc tacatctccg
    12721 agatttcctg gcacgtgacc ccgttccggt cgcagcgctg gatcgacacc tgggagccgg
    12781 ctgccgccaa ggcaaccgaa tacggcgcca cggaatggga gatctaccgc tcggacgagg
    12841 acccgctgct ctttcggcag gtgactcgct gggagaagaa gtcggacttc gaagcctggt
    12901 ggtactcgga ggagatttcg gcgatccgcg ccgaggtcgt ggacctctac gacaagcccc
    12961 tcctccccgt ctggtgctcg cgggtcatag cctcaagctg accaaagcac cacgcaaagc
    13021 atcaaaaaag ggctccggaa atccggggcc cttttctttg caggcgttgg tggtcacgcc
    13081 gtggggtcgg gtgggcgtca ccgggatact caggcggagc gcggctgaat gccgcgcgag
    13141 gtgtttgccg cttcccggtg atgcccaccc gaccccacgg ctactcgaga tcgcggagcc
    13201 acttccccat cgcctgccct ttcagacccc gaggtgccgc acattcagga ccgtgatggg
    13261 cctacacgtg cggcaccttc cgccgctcgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13381 nnnnnnnnnt gccgcacatt caggaccgtg atgggcctac acgtgcggca ccttccgccg
    13441 ctcgctcgac ttttccggcc gccccccacc tgccctgcag acttcgaggt gacgcaggtt
    13501 tgcgacctct ccggtagcaa acctgcggca cccttccaga gctggacctc agtatgggtc
    13561 ggggattgct acgcggatgt cggcgaagga gccgatcgcg ccgcgggcgc tctgcttggc
    13621 gccgatcgag atgctcgtgt cctgcccgac cagtgaggtg ccctggtcgt cacgggcggc
    13681 ggcaaccttg tggccgttga cgaagccaac cagctgggtg ttgcccttct ggccggtgaa
    13741 ggcgcgaagg gtcaggcggt tcgccttgcc gggacccttc accttgcggc cggccttgcg
    13801 actgccgaag ttgatgatct gcccgttcgg ctggaccttg cgcaggccgt agcggcgcgt
    13861 ggagggccag acaaccagct ggtagcggct gccgtcgttg ttctgacgga ggctgacccc
    13921 ggcatatgcg cggcgcagga ccttgggcgg ggtcgacttg aaaatcctgg cgatcgcggt
    13981 gacctgcagg ttcttgccgg ccatcgggac cttgtagaag cactccctcg ccttcttccc
    14041 gagctggaag cggaaggcgt tgggggaacc gccccgttcg cagttggcgc gcggcgccat
    14101 ctgatggatt tcggcacggc tgtcagtggt gtcgaggtcg ttcttgtaaa tcgtgattgt
    14161 gctggcggcg gcgttggcag cgaaaagcac cgcgccaagc gacaccgcgg ccacgatggc
    14221 acgcagcagg gggtttttgg tcagggagcg catgtgaaga gcctaaacac agaaagtggc
    14281 tgaatggtgc gccctgccgg tcctgcttca aatgctgcgt gacgaaatca cacaatccgg
    14341 gaacggtccc gccgggcccg ccgcgaaggc gggaaaccgc gactcgcagg tagaatcggc
    14401 tccatgagcg accaccgtct gcccgaacgc gatcgcccct gggtgatgcg cacttacgcg
    14461 ggccaggagc gtgggcggaa ggctgcgcgc gggactggag gagatccaga gccgtcaccg
    14521 cagcgtgggc gacgtgcgcg gacgcggcct gatgctgggc gtggaactcg tggagccgga
    14581 cggcggaaag accccggcgc cagcgcgggc
//
LOCUS       DAOQKN010000043        14507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_1931_length_14507_cov_6.604830, whole genome shotgun sequence.
ACCESSION   DAOQKN010000043 DAOQKN010000000
VERSION     DAOQKN010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 14507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14507
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1931_length_14507_cov_6.604830"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="PLJ64_04545"
     CDS             complement(<1..547)
                     /locus_tag="PLJ64_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HNC92791.1"
                     /translation="MRELRPGTVIAVIALFIAIGGTATAASGLINGSKIKPGTVTARQ
                     IKNQTITTSKLAPATVASLRGARGPVGATGPAGENGQPGTPGQPGQPGAVGATGATGA
                     TGADGVVEPYMDQVLNFNLPTEDFTIPLSIDLPPGQYLLTAKTNVVNQYASGDNMIEC
                     TIWTDEVDGVDRATSDVPYNRI"
     gene            721..2070
                     /locus_tag="PLJ64_04550"
     CDS             721..2070
                     /locus_tag="PLJ64_04550"
                     /inference="COORDINATES: protein motif:HMM:NF021427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HNC92792.1"
                     /translation="MHDIECPHCGKAFTIDEAGYADILNQVRDEAFEKAIHDRLELAE
                     REKLAAVELARTKAASEHKDAATEKDLEIERLREELKASSELARAKATGELKDESAKK
                     DAEIERLKAELDKADVAGKLALKEALGEVEKERDDLKRSLESKDTEQKLLETSLKEKY
                     EIQIKDRDGEIERLKDMKAKLSTKMIGETLEQHCEIEFNKIRAAAFPNAVFGKDNDAS
                     AGTKGDFIFREADPEGTEFASIMFEMKNELDDGVNKKKNEDFFRKLDDDRKKKGCEYA
                     VLVSLLEPENELYNAGIVDVSHLYPRMYVIRPQFFIPIITLIRNEALKTLKLRSELAQ
                     VRAQNVDITNFEEEMENFKSGFQRNFGIASDQFDKAIEQIDKSIANLGKTKDWLLKSA
                     NQLRLANDKAQDLSIKKLTRGNETMKAKFEEARSPDLEEIKRLEIAESDSGPDSRAA"
     gene            2138..2815
                     /locus_tag="PLJ64_04555"
     CDS             2138..2815
                     /locus_tag="PLJ64_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HNC92793.1"
                     /translation="MKTEIRHNPGFAIARCTLAPGEQMRAESGAMAATSAGVEIEAKV
                     QGGLMKGLKRSVLGGESLFMTTLTAPQSGGWVDVAANLPGDAMVMSTGEELFLTRGTY
                     LASDASVDIDTKWGGFKNIGGGEGGFLIRATGGGEVVVSCYGAIDRIELGAGEQIVVD
                     SGHLVAFEPSVQYKTRKASSGLMNTLKSGEGFVMEFTGPGVIHTQTRNPDFLVSWLTE
                     VLPFTRA"
     gene            complement(2850..3737)
                     /locus_tag="PLJ64_04560"
     CDS             complement(2850..3737)
                     /locus_tag="PLJ64_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNC92794.1"
                     /translation="MEAAAKNGTPVVELTPGQSFTGRYACARKDRLTAKNGSAYLALE
                     LRDRTGSIPARIFRDSDRIGINFERGDVVAVKGRVEQFRGQLSAEIEDAKKIDPSEID
                     PADFLPTAYRDREELEGFLEHLAREVHHPGYRAVVEDLLFTGETAEAFRLAPCTRAGH
                     HAYIGGLSEHTVAVGTLVTETCVLHPKLNSDLLMAAALIHDIGKTGEFTYGAEIGLSE
                     RGRNLGHLQIGAELVGEAADRAGLDQAERDALLSTVMCHHGTDPLRLRQFPSAEAIAL
                     YRLNALDAQVKTALEHGLT"
     gene            3839..4702
                     /locus_tag="PLJ64_04565"
     CDS             3839..4702
                     /locus_tag="PLJ64_04565"
                     /inference="COORDINATES: protein motif:HMM:NF021287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HNC92795.1"
                     /translation="MDQLRWEMNPPKLRDPMMVCAFRGWNDAASAASTALETLADSLE
                     VDVLAEVDPEDFYDFQANRPTIRLEEGAHRRIEWPGNTFIAARAQGAERDLVLLDGTE
                     PNLKWRTYSEMVVQVAEQLDVQLVVVLGSLIAEVAHTLPVPITGVSTDEQTVRSLELE
                     RSDYEGPTGIVGVIHDRCRAAGLPSVSLWAAVPHYVAAVPNPKASLALSEKLESITRV
                     AADISGLEDETMNYEEQIGRAVAADPEVEELVERIEKEQRQRRGPELDVPSGDALAME
                     FQRFLKNRDSD"
     gene            complement(4715..7774)
                     /locus_tag="PLJ64_04570"
     CDS             complement(4715..7774)
                     /locus_tag="PLJ64_04570"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HNC92796.1"
                     /translation="MSDSSSRTGADGDPGPGRELVRRHGSGALLIRGGAGTGKSTALR
                     ERVIHLARPETGRPGLDPCRVALIASTDRTAAAHRIRLEDDLPGPYEALSVFTWEGLA
                     EEILRHRPVEAGLGPSFEVVGAAERLAMLLARVDELPLRHQEIRGNPAGLLRELLIGI
                     DRAKREGTDGGELAELIEIHNRILAAADLLDRNELPRIATDLLADPALAAEVTARFPH
                     LVIDELEDLHPARAGLLQRLAVAGPESVVAGIDPSRAIYGPGAEERFLAAFPDLEQFT
                     LDEAWRMSEVRITAARAALGPGPLPEGAWEARGDEGSVRYWRPASARAEAQAAAREVE
                     HQIALGTPPEQLAIAVPDVNSQGVGVVGALAERGIPARPGGGAALFRQPEVRDTIAWL
                     RALTDPTDARAVTRALMRPPTELRSLDLARLTQISKRRKLDMISACEASLESPQMAPE
                     ARQRIEGFLNLYRAASGALDTRRPDAYVRRLIERIGFRRQRLFAARPETAERLLGLSR
                     LAQIATDFSRRDPNASVREFTVFLEALADGGLEPVGGPDLPGVGAVPVMNLDLLKGGE
                     WARIWVLGLERPFGITRNPGPGIGDDQTHPGGPETARVAVATALTSAREEVVLSRASE
                     AEPDPVAAILEATGGLEEIQGEELFGPAEDVAATYRAMLGEVMEASWKAGSELNEPRL
                     DTAIDVNRAVARYLELLKLAALAQRDDGSSDEEAIEAVNGLIAQVATPEQLAELDSST
                     LDPYLLAQERERGMRQGLADSRSEPSLDAFLPRRGDDLRLSATDLDMYLTCPLKYKFA
                     RVFGIPTPPTVNMRFGIVIHNVLQRFHELEQDVGRPVPDGEARLMALLEEGWRRGGFG
                     ETHDELQFLDRAREAMRAYWRSEEASESEPAFLERQFEFKVGPHFLRGRVDRVDRKPG
                     GEFEVIDYKTGQRIDSSRHGGDIQLALYRLGAREAWGIEPEAGSYYYVLEGEKVEVQA
                     SPDDRERVERTVLEVGEGVLGQDFEPRPSPGVCGWCDFRLVCPAAEA"
     gene            7828..8667
                     /locus_tag="PLJ64_04575"
     CDS             7828..8667
                     /locus_tag="PLJ64_04575"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNC92797.1"
                     /translation="MNQLPPDPALDADWTGICRHIVEEQKKLFAEYETIQQRDNYEGV
                     GEGGDHALVLDRMCEDVIFAELEKLADSGGAAFTAISEERGTVLFNEGGNVWVVIDPI
                     DGSLNFKRTIPQHSLSIAVATAPNMGAVEFGYVYDFGTGDEFLAQSGEGAYLNEERIN
                     LEPREGLEVVGIEGADPTALAPMLAELAGKVYRVRCVGSLAISIASVAAGRFDALAFP
                     HLSRSVDVAAAQLIAREAGALVDLGDGGLSEVGLALEDRFPVTVAATGEGLETLVSIR
                     TVG"
     gene            8840..9460
                     /locus_tag="PLJ64_04580"
     CDS             8840..9460
                     /locus_tag="PLJ64_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92798.1"
                     /translation="MAEAKKTTKKSTAKKTAAKKPAAKKRTAAQLRAEAEKKAIHAAK
                     LDAENAKSRLEEVQETAVEYAKKGVDVPVGAALNLADRVSEVIEDWSDQASAEKKVKG
                     YRADLTKTVKRAERRGITTRRKATTQARKQRKSLEKTVRQQRKSIEKTVKTTNKDVNK
                     QIEARVNEVTKRANQVQADVTKVADEQGKKAQDLVNKVTEQLSALV"
     gene            complement(9604..10005)
                     /locus_tag="PLJ64_04585"
     CDS             complement(9604..10005)
                     /locus_tag="PLJ64_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92799.1"
                     /translation="MVVALIALTIAIGGTAAALPGKFTVGRDDLKTSSVGARALGKIV
                     ISHSQVFPSADPTAQDGNFTETIGTITCPDDAPTAIDPFVGGMSTTAYVFGRTAIFNR
                     FRAPQGYRYTVFSDDGPEVGYVLKVNCIPAR"
     gene            complement(10057..12906)
                     /locus_tag="PLJ64_04590"
     CDS             complement(10057..12906)
                     /locus_tag="PLJ64_04590"
                     /inference="COORDINATES: protein motif:HMM:NF039533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNC92800.1"
                     /translation="MNGFQKLAVVILGVLALGLVGASGANAGTYVMRQCDGSSFLDFQ
                     GTYSAINASPHFDAVSGCVTSGSGKLGIYQDRSGTSLAYGEGGHFVWAAPTGTEVIGA
                     RIAARLNDKNNIQAQLNGYNVGVHTDIDGGIPHDGSDQVSYWSDPAHPQWMVVIELLC
                     QSVNGCANQVGGTKAFLEVTDTEFTVRDTVAPTAAASGTLWDWGGDSGYHRGTASIGI
                     DATDQGSGVAATWVEINGLRVDLPAPTCPGDKGDYTTRFTPCPLTYSTIRTFDTSVSP
                     FQEGSNQIRVCSRDYASSEANASKTCSATRTVLIDNRFSNPADNLRSDQGTDWQPVNG
                     FTLRWEIPEGQVAPIIGAVYKIQELETDTLVDAGYFSGNDIQSAGPINLPAVGAYKVI
                     IHLVDGALNLGRDAETILRFDDRPPGDVSPVEPEGWISRDELPIQQEIEKAAPGGPSG
                     ISGYALEVSDDGPAQPCGSEICLAPEITLAGGPDLRTGSIGGLVEGNHWISAAAVSGA
                     HKSSVEPGSTMVQVDRTPPTVSISGVPNEWVNHPVTLTAQASDQLSGMQPQPADDGSP
                     KTVIDADNYAAYESPGPFASFSVATEGVNRVRYWAEDLAGNANDGMPGHDGDTHATPG
                     QAVVRIDTTPPEVRFDPARDPEDPEVVRISADDTDSGVASASIGIRRAGSGETFIQLQ
                     TAGADGQYQARIPSDDLAQGAYELNATVEDRAGNESTGNRTSAGDPMIVNLPVKQPAS
                     IAASLTGGKSNILARYGSARYLEGRLTSGETALSNQTVTAIETFKAGSSRNAVSRDLV
                     TDGDGRFRVLLTPGPSRTIEVTYAGTRMISRTAGPKLGMRVKGKVTLKIKPKKVLNGG
                     VVRMSGKVGFAGVLPPSRGKLVAIQYFDPSRRKWRPVEVLRTNRRGLFRYRYRFRTIT
                     SAQRILFRASALPEAGWPYLPSTTKPKSVIVYPKD"
     gene            complement(12903..14258)
                     /locus_tag="PLJ64_04595"
     CDS             complement(12903..14258)
                     /locus_tag="PLJ64_04595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNC92801.1"
                     /translation="MRGVAVQGTEEHDAGIRPDEAARKRVAVELIRNHDRPLRWTARR
                     YSICPEDAEDAYQRGIEILLSKAPTTDTRHLLPWTRKVIKHEALAIRRTRERTLARPG
                     QSDIDEHLEDDWVQLIPDISDGPDELTVRRERVARSREALTQLKPQELKALTQLAEGF
                     SYDEIGSMNSWTRTKVNRCLAEGRARFRSTFRESEAGERCPEFEPMISASCDGELTSS
                     QVAELETHLAVCGHCRSTLRAYRAAPRAAAALAPLLPAGRGLWERTQELAVDLQTRLS
                     GVGRGSDSGVQTVLASGGTRGAGMAAIAKIAAICAGTAGTAAVCAATGVLPAANLLPH
                     KDAKPVVERHASVARPEPERRKLDFVEPAPEPSTAPQSQAGGSPDQSATAAEPVSTTP
                     EPVSEAPVITESAPAPTPTETEFTPEAAGTPAPAAAPPPQPASSSSSVPVGNGGGEFS
                     P"
BASE COUNT         2518 a         4717 c         4754 g         2518 t
ORIGIN      
        1 agatcctgtt gtagggcacg tcgctcgtcg cccggtccac accgtcgacc tcatcggtcc
       61 agatggtgca ctcgatcatg ttgtcgcccg atgcgtactg gttcaccacg ttggtcttgg
      121 cggtgagcag gtactggccg ggaggcagat cgatcgagag cgggatcgtg aaatcctcgg
      181 tcggcaggtt gaagttcagg acctgatcca tgtacggctc gaccacgccg tcggcgcccg
      241 tggctccggt ggctccggtc gctccgaccg cgcccggctg acccggctgg cccggggtgc
      301 ctggctgacc gttttccccc gccggcccgg ttgctccgac cgggccacgg gcgcctcgca
      361 gcgaagcgac cgtcgccggc gcgagcttgg aggtggtgat cgtctggttc ttgatctgcc
      421 tggcggtgac ggttcccggc ttgatcttgc ttccgttgat cagtcccgac gctgccgtcg
      481 cggtgccccc gatcgcgatg aacagcgcga tcacggcaat gaccgtgccc ggtcgtaatt
      541 ccctcattct tgctccttgc tggttctttc cctgcctgcc ctggagccga atgcggccgt
      601 gccggcggtc ttcaagcgtg gagcatgtca gaacccgcaa tccgaaggcg actcagtctg
      661 agcatcctga ccaccgccta agctgaaggg ccgtcggcga aaccgaaagg agccttggaa
      721 atgcatgaca tcgaatgccc gcattgcggg aaagccttca ccatcgacga ggccggttac
      781 gcggacatcc tcaaccaggt tcgtgacgaa gccttcgaaa aggcgatcca tgaccggctg
      841 gaactcgccg aacgggagaa gctggcggcg gtcgagctgg cccggaccaa ggcggccagc
      901 gagcacaagg acgctgccac cgagaaggac ctggagatcg agcgcctgcg ggaagagctg
      961 aaggcgagtt ccgaactggc cagggccaag gcgaccggag agctcaaaga cgaaagcgcg
     1021 aagaaggacg ccgagatcga acgcctgaag gcagagctgg acaaggcgga cgtcgccggg
     1081 aagctcgccc tcaaggaagc cctgggcgaa gtcgagaagg aacgcgacga cctcaagcgg
     1141 tcgcttgagt ccaaagacac cgagcagaag cttctcgaga cttcactcaa ggagaagtac
     1201 gagatccaga tcaaggaccg cgacggcgag atcgaacgcc tgaaggacat gaaggcgaag
     1261 ctctcgacca agatgatcgg cgagaccctg gagcagcact gcgagatcga gttcaacaag
     1321 atccgcgccg ccgctttccc gaatgccgtg ttcggcaagg acaacgacgc cagcgccggc
     1381 accaagggcg acttcatctt ccgcgaggcc gacccggaag gaaccgagtt cgcctcgatc
     1441 atgttcgaga tgaagaacga gctcgacgac ggcgtaaaca agaagaagaa cgaggacttc
     1501 ttcaggaagc tggatgacga ccggaagaag aagggctgcg agtacgccgt cctggtctcg
     1561 ctgcttgaac cggaaaacga gctctacaac gccggcatcg tcgacgtttc gcatctctat
     1621 ccccgtatgt atgtgatccg gccacagttc ttcatcccga tcatcacgct gatccggaat
     1681 gaagccctca agaccctgaa gctcagatca gagctggccc aggtgcgggc ccagaacgtc
     1741 gacatcacca acttcgagga ggagatggag aacttcaaga gcggcttcca gcgaaacttc
     1801 gggatcgcct cggatcagtt cgacaaggcg atcgaacaaa tcgacaagtc cattgcaaat
     1861 ctcggcaaga ccaaggattg gctgctcaag tctgccaacc agttgcgtct tgccaacgac
     1921 aaggcccagg acctgtcgat caagaaactc acgcggggca acgaaacgat gaaggccaag
     1981 ttcgaggaag cccgctcgcc ggatctcgaa gagatcaagc ggctggaaat cgccgagtcc
     2041 gattccgggc ccgactcccg ggctgcctga gttctccgga actcccccgg aactgttgtc
     2101 cggcgtttgc cttcgcggtg acggctaggg tgggcgcatg aaaactgaga ttcgtcacaa
     2161 ccccggtttt gcaattgctc gatgcacgct cgcccccggc gagcagatga gagccgagtc
     2221 gggggcgatg gccgccacct cggccggagt cgagatcgaa gccaaggtgc agggcggact
     2281 gatgaagggg ctgaaacgtt cggtgctcgg gggcgagtcg cttttcatga ccaccctgac
     2341 cgcgccgcag tccgggggct gggtcgacgt cgcggcgaac ctgcccggcg acgcgatggt
     2401 catgtcaacc ggggaggaac tcttcctcac ccgcggtact tatctggcct cggacgccag
     2461 cgttgacatc gacaccaagt ggggcggctt caagaacatc ggcggcggcg agggcggctt
     2521 cctgatccgc gcgaccggcg gtggcgaagt ggtcgtctcc tgctacggcg cgatcgaccg
     2581 gatcgaactc ggcgcgggcg agcagatcgt ggtcgactcc ggtcatctgg tcgccttcga
     2641 gccgagcgtt cagtacaaga cgcgcaaggc atcctcgggc ctgatgaaca ccctgaagag
     2701 cggcgagggt ttcgtcatgg agttcaccgg tcccggcgtc atccacaccc agaccaggaa
     2761 cccggacttc ctggtcagct ggctgaccga agtcctgccc ttcacccggg cctagccgcg
     2821 accggccgct ggaggttccc gcttttcgct caggtcaggc cgtgttcgag tgcggtcttg
     2881 acctgggcgt cgagcgcgtt cagccggtag agggcgatcg cctcggccga ggggaactgg
     2941 cgcaaccgca gcggatcggt gccgtggtga cacatcacgg tcgaaagcag ggcgtcccgc
     3001 tcggcctgat cgagaccggc ccggtcggct gcttcaccga ccagctccgc cccgatctgg
     3061 aggtggccga ggttgcgtcc gcgctcgctc aggccgatct cggccccgta ggtgaactcg
     3121 ccggtcttgc cgatgtcgtg gatgagggcc gcggccatca gcaggtcgga gttgagtttc
     3181 ggatggagca cgcaggtttc ggtgaccagg gtgccgaccg cgacggtgtg ctctgaaagc
     3241 ccgccgatgt aggcgtggtg gccggcccgg gtgcaggggg cgagccggaa ggcttcggcg
     3301 gtttcgccgg tgaacaacag gtcctcgacc accgcccggt agccggggtg atggacttcc
     3361 cgggcgaggt gttcgagaaa gccttcgagt tcttcccggt cgcggtaggc ggttggcaga
     3421 aagtcggccg ggtcgatctc cgacgggtcg atctttttcg cgtcctcgat ttcggccgag
     3481 agctgcccgc ggaactgctc gacccggccc ttcaccgcga ccacgtcacc acgttcgaag
     3541 ttgatcccga tgcggtcgga gtcgcggaag atccgggcgg gaatcgagcc ggtccggtct
     3601 cgcagttcca aggcgaggta ggccgatcca ttctttgcgg tgagccggtc cttgcgggcg
     3661 caggcgtagc ggccggtgaa gctctggccg ggggtcaatt cgaccaccgg ggttccgttt
     3721 ttggccgcgg cctccatccc ccacatcctg accgaagccg cagacgggcg ggacgccgcc
     3781 gagctgccac cggtcgcagg cggcagggcc gggagagcgg gtcggctaaa ttgaactaat
     3841 ggaccaactc cgttgggaga tgaacccgcc aaagctccgt gacccgatga tggtctgcgc
     3901 ctttcgcggc tggaatgacg cggcgagtgc cgcctcgacc gccctggaga ccctggccga
     3961 ttctctcgag gtggacgtgc tggccgaggt ggacccggag gatttctacg actttcaggc
     4021 gaaccggccg accatccgcc tggaagaagg agcccaccgg agaatcgaat ggccgggcaa
     4081 caccttcatc gccgcccgcg cccagggtgc cgaacgggac ctggtcctgc tcgacgggac
     4141 cgaaccgaac ctgaagtggc ggacctactc cgagatggtg gtccaggtcg ccgagcagct
     4201 cgacgtccag ctggtggtcg tgctcggctc actgatcgcc gaggttgccc acaccctgcc
     4261 ggtcccgata acgggggttt caaccgacga gcagaccgtc cgcagcctcg agctcgaacg
     4321 ctccgattac gaaggcccga ccggcatcgt cggcgtgatc cacgaccgct gccgggccgc
     4381 cggcctgccc tcggtttcgc tctgggcggc ggtgccccat tacgttgcgg cggtccccaa
     4441 cccgaaggcg tcgctcgcgc tcagcgagaa gctcgaatcg atcacccggg tcgccgccga
     4501 catctccggt ctcgaagacg aaactatgaa ttacgaggag cagatcgggc gggcggttgc
     4561 cgccgacccg gaggtcgagg aactggtcga gcggatcgag aaggaacagc gccagcggcg
     4621 cgggccggag ctcgacgtcc ccagcggcga tgcccttgcg atggagttcc agcggttcct
     4681 caagaaccgc gattcagact gacctttgcc gggctcaggc ttcggcggcc gggcagacca
     4741 gcctgaagtc acaccagccg cagaccccgg gcgaggggcg cggttcgaag tcctggccga
     4801 gcaccccttc gccgacctcg agcacggtcc gctcgacccg ctcgcggtca tccggtgagg
     4861 cctggacctc gaccttctcg ccttcgagca cgtagtagta gctgccggcc tccggttcga
     4921 tcccccaggc ctcgcgcgcc ccgagccggt agagggcgag ctggatgtca ccgccgtggc
     4981 gggaggagtc gatccgctgg ccggtcttgt aatcgatcac ctcgaactcg ccaccgggct
     5041 tgcggtcgac ccggtcgacc cggccacgaa ggaaatgcgg cccgaccttg aactcgaact
     5101 gccgttcgag gaaggccggc tcggattcgg aggcctcttc gctcctccag taggcccgca
     5161 tcgcctcgcg ggcccggtcg aggaactgaa gctcgtcgtg ggtctcgccg aaaccaccgc
     5221 gacgccagcc ttcctcgagc agggccatca accgggcctc gccatccggg accggccggc
     5281 cgacgtcctg ttccagttca tggaagcgct gcaggacgtt gtggatcacg atcccgaagc
     5341 gcatgttcac ggttggcggt gtggggatgc cgaagacccg cgcaaacttg tacttcagcg
     5401 ggcaggtcag atacatgtcg aggtcggtcg ccgagagccg gaggtcgtca ccgcgccggg
     5461 gcaggaaagc atcgagcgat ggttccgagc gggagtcggc gaggccctgc cgcatgcccc
     5521 gctcgcgttc ctgggcgagc agataggggt cgagggtgga ggagtcgagt tcggccagct
     5581 gctccggggt ggcgacctgg gcgatcaggc cgttgaccgc ttcgatcgcc tcctcgtcac
     5641 tggatccgtc atcgcgctgg gccagggcgg ccagcttcag gagctcgagg tagcgggcga
     5701 ccgcgcggtt cacgtcgatc gccgtgtcga gccgtggttc gttcagctcg gagccggcct
     5761 tccaggaggc ctccatcacc tcgccgagca tcgcccggta ggtcgccgcg acgtcctcgg
     5821 ccggtccgaa gagctcttcg ccctggattt cctcgaggcc gccggtcgct tcgaggatcg
     5881 ccgcgaccgg gtccggttcg gcctcgctgg cccgcgagag aaccacttct tcccgggccg
     5941 aggtgagtgc ggtggcgacc gcgacacgcg ccgtttcggg gccgccgggg tgggtctggt
     6001 catcgccaat gcccgggccg gggttgcggg tgatcccgaa cggacgctcc agaccgagca
     6061 cccagatccg ggcccactcg ccgcccttga gcaggtcgag gttcatcacc ggcaccgctc
     6121 cgactccggg cagatccggc ccaccgaccg gttcgaggcc gccgtcggcc agcgcttcca
     6181 ggaagacggt gaactcccgc accgaggcgt tcgggtcccg gcgggagaag tcggtcgcga
     6241 tctgggccag ccgggagagg ccgagcaggc gttcggcggt ttcgggccgc gcggcgaaga
     6301 gtcgctggcg gcgaaaaccg atccgctcga tcagccgcct cacataagcg tcgggccggc
     6361 gggtgtcgag cgcacccgag gctgcccggt agaggttgag gaagccttcg atccgttgcc
     6421 gggcctccgg cgccatctgc ggtgattcga gcgaggcctc gcaggcgctg atcatgtcga
     6481 gcttgcgtcg tttcgagatc tgggtgagcc gggccaggtc cagggagcgg agctcggttg
     6541 gcggccgcat gagggcgcgg gtgaccgccc gggcgtcggt cggatcggtc agcgccctca
     6601 gccaggcgat cgtgtcgcgc acctccggct gccggaaaag cgcggcgcca ccgccgggcc
     6661 gggcggggat gccccgttcg gccagcgccc cgaccacccc gactccttgc gagttcacgt
     6721 ccggcaccgc gatcgccagc tgctccggcg gagttcccag ggcgatctgg tgttcgacct
     6781 ccctggccgc ggcctgtgcc tccgcccggg ccgaggcggg ccgccagtag cggaccgagc
     6841 cttcatcgcc gcgagcttcc caggctcctt cgggcagggg gcccggacca agtgcggccc
     6901 gggcggcggt gatccgcacc tcgctcatcc gccaggcctc atccagggtg aactgctcca
     6961 ggtccgggaa ggcggccagg aaccgttcct cggcgcccgg cccgtagatc gcccgggagg
     7021 ggtctatgcc cgcgaccacg ctttcgggcc cggccacggc cagtcgctga agcagcccgg
     7081 cccgggccgg gtgaaggtcc tcgagctcgt cgatcaccag gtgggggaag cgggccgtga
     7141 cctcggcggc gagggccgga tcggccagca ggtcggtggc gatgcgcggc agttcgttgc
     7201 ggtcgagcag gtcggccgcg gcgaggatcc ggttgtggat ctcgatcagt tcggccagct
     7261 cgccgccgtc ggtgccctcc cgtttggccc ggtcgatgcc gatcagaagc tcccggagca
     7321 gcccggccgg gttgccccgg atctcctggt ggcggagcgg cagctcgtcg acccgggcca
     7381 gcagcatggc caggcgttcg gccgcgccga ccacctcgaa ggagggtccg agccctgcct
     7441 cgaccggccg gtggcggagg atctcttcgg ccaggccctc ccaggtgaag accgaaagcg
     7501 cttcataggg cccgggcaaa tcatcttcga gccggatccg gtgggcggcg gcggtgcggt
     7561 cggtcgaggc gatcagggcg acccggcacg gatcgaggcc cggccggccg gtttccggcc
     7621 gcgcgaggtg gatcacccgt tcccggaggg cggtgctctt gccggtaccc gcgccgcccc
     7681 gaatcaggag cgccccggag ccgtgcctgc gaaccagttc ccggccgggg ccgggatctc
     7741 cgtcggcacc ggttctggac gaggaatcgc tcaccccctc aaattacccg agcctcgtcc
     7801 tgtttcacag cgtctagcat gtcgcgggtg aaccaacttc ccccggaccc cgcactcgac
     7861 gctgactgga cggggatctg ccgccacatc gtcgaggaac agaagaagct cttcgccgag
     7921 tacgagacga tccagcaacg cgacaactac gaaggcgtcg gcgagggagg cgaccatgcc
     7981 ctggtcctcg accggatgtg cgaggacgtg atcttcgccg agctggagaa gctcgccgat
     8041 agcggcggcg ctgccttcac ggcgatatcc gaggagcgcg ggaccgtcct gttcaacgag
     8101 ggcggcaacg tgtgggtcgt gatcgacccg atcgacggtt cgctcaactt caagcggacc
     8161 atcccccagc acagcctttc gattgcggtc gccaccgccc cgaacatggg ggcggtcgag
     8221 ttcggctatg tctatgactt cggaaccggg gacgagttcc tcgcccagtc cggcgagggc
     8281 gcctacctga acgaggagcg catcaacctg gaaccccggg aggggctcga ggtggtcggc
     8341 atcgaggggg ccgaccccac cgcactcgcc ccgatgctgg ctgaactggc cggcaaggtc
     8401 taccgggtgc gctgtgtcgg ctcgttggcg atctcgatcg cttcggttgc cgcgggccgt
     8461 ttcgacgcgc tcgccttccc ccatctctcc cgctcggttg acgtggcggc ggcccagttg
     8521 atcgcccgcg aggccggcgc cctggttgac ctcggggacg gcggtctgag cgaggtcggc
     8581 ctcgccctcg aggaccgttt cccggtcacc gtggccgcca ccggcgaggg tctggagacc
     8641 ctcgtttcga ttcgcaccgt cggctgaatc ccggattgcg tccgattccg gcgctgtttt
     8701 ccgctctgcc atgcggattc tccctgcgtg accgaggtca cacgaaaaaa gaacatgtgt
     8761 tcgtgtttgg ttgctacact tcaaacatcc ttagaagtaa gtaatctgat ctcaacaaac
     8821 aaaggactaa ctgatcccca tggccgaagc caagaagacc accaagaaga gcaccgccaa
     8881 gaagaccgcc gcgaagaagc cggctgccaa gaagcgcacc gccgcccagc tccgcgccga
     8941 ggccgagaag aaggccatcc acgccgccaa gctcgacgcc gagaacgcca agagccgcct
     9001 cgaagaggtc caggagaccg ccgtcgagta cgccaagaag ggtgttgacg tgccggtagg
     9061 cgccgccctc aacctggccg atcgcgtttc cgaagtgatc gaggactggt ccgaccaggc
     9121 cagcgccgag aagaaggtca agggctaccg cgccgacctg accaagaccg tcaagcgcgc
     9181 cgagcgtcgt ggcatcacca cccgtcgcaa ggcgaccacc caggcccgca agcagcgcaa
     9241 gtcgctcgag aagaccgtcc gccagcagcg gaagagcatc gagaagaccg tcaagacgac
     9301 caacaaggac gtcaacaagc agatcgaagc ccgcgtgaac gaagtcacca agcgcgccaa
     9361 ccaggttcag gctgacgtca ccaaggtcgc cgacgagcag ggcaagaagg cccaggatct
     9421 ggtcaacaag gtcacggagc agctttcggc tctggtctag accaaaccgc ttagcccggc
     9481 cgccgcagga gaacggcgaa ccggtgaaga tcggctgacc tcataacctc gtcagtcgat
     9541 gtagtacctc tcctccctcg ccaacggccc ctcctcgggg ccgttggtcc ttaagtgatg
     9601 tgatcaacgg gcagggatgc agttgacttt caacacgtac ccaacttcgg gtccgtcgtc
     9661 gctgaagacg gtgtaccggt agccctgggg ggcacggaac cggttgaata ttgcggttct
     9721 gccaaagaca taggccgtag tgctcatccc gccaacaaaa ggatcgatcg cagttggcgc
     9781 atcgtcgggg caagtgatcg ttccgatggt ctcggtgaaa ttgccgtctt gggcggtcgg
     9841 atccgctgag gggaagacct gagaatggct aattacgatc tttcccaacg ccctggctcc
     9901 gacactggag gtcttgagat catcccggcc cactgtgaac ttgccgggca gggcagcggc
     9961 ggttccaccg atcgcgatcg tcaaggcgat cagtgccacc accattgccg gcgacggtct
    10021 ccgtatccct ctcatcctgc ctccttccct gccgaatcag tccttcgggt aaacaatgac
    10081 cgatttcggc ttcgttgtcg agggcagata gggccagcca gcctcaggca gggcgctggc
    10141 cctgaacagg attcgctggg ccgaggtgat agtccggaag cggtaccggt accggaaaag
    10201 tcctcggcgg ttggtccgga gcacctcgac cggtcgccac ttgcggcggg aagggtcgaa
    10261 gtactggatt gcgaccagct tgccccggga cggcgggagc accccggcaa aaccgacctt
    10321 gccggacatc cggacgacgc ccccgttgag cactttcttc ggcttgatct tcagcgtcac
    10381 cttgcctttc accctcatgc cgagcttcgg tccggcggtc cgggagatca tcctggtgcc
    10441 cgcgtatgtg acttcgatcg tccgcgacgg gcccggggta aggaggaccc tgaagcggcc
    10501 gtctccgtcc gtaaccagat ccctcgaaac cgcgttccgg ctcgaaccgg ccttgaacgt
    10561 ttcgattgcc gtgaccgtct ggttcgacag cgccgtttcg ccgctggtca acctgccttc
    10621 caggtaacgg gcgctgccat agcgcgcgag gatgttcgac tttccgccag tgagcgacgc
    10681 ggcaatcgag gcgggctgct ttacgggtag gttgacgatc atcggatcgc cggccgaagt
    10741 ccggtttccg gtcgattcgt ttccggccct gtcctcgacg gtcgcgttca gttcatacgc
    10801 gccttgcgcc aggtcatcgg aagggatcct ggcctggtac tgaccgtcgg cccccgcggt
    10861 ctggagttga atgaatgtct ctcccgaccc cgccctccgg attccgatcg aagcagaggc
    10921 gacgcccgag tcggtgtcat ctgcggagat ccggacgact tccggatcct ccggatccct
    10981 ggctggatcg aatctgacct ctggcggggt ggtgtcgatc cggaccaccg cctggcccgg
    11041 agtcgcgtga gtgtcgccgt catgtcccgg catcccgtcg ttcgcgttgc cggccagatc
    11101 ttccgcccag tagcggaccc ggtttacgcc ttcggtcgcc accgagaaac tggcgaaagg
    11161 tccgggtgat tcgtaggccg cgtagttgtc ggcatcaatc accgtttttg gactgccgtc
    11221 gtcagccggc tggggctgca tcccggagag ctggtcgctc gcctgggcgg tcagggtgac
    11281 cggatggttg acccattcgt tcggaactcc ggatatcgag acggtcggtg gagtccggtc
    11341 gacctgaacc atcgtgctgc ccggctcgac cgaggacttg tgagcccccg agaccgcagc
    11401 cgccgaaatc cagtgattac cctcgacaag gccgccgatc gaacccgtcc tcaggtccgg
    11461 gcctccggcc agggtgatct ccggggcgag gcagatctcg gatccgcagg gctgcgccgg
    11521 cccgtcgtcg gacacctcca gcgcgtagcc actgattccg gacgggccac cgggcgctgc
    11581 cttttcgatc tcctgctgaa tcggcagttc gtcccttgag atccatccct ccggttcgac
    11641 cggactcacg tcacccggcg gcctgtcgtc gaagcggagg atggtctcgg cgtcccgtcc
    11701 gaggttgagc gctccgtcga ccagatggat gatcaccttg tacgcgccga ccgcgggcag
    11761 gttgatcgga ccagcgctct gaatgtcgtt tccgctgaag tagccggcgt cgaccagcgt
    11821 gtcggtctcg agttcctgaa ttttgtagac ggccccgatg atcggtgcga cctggccttc
    11881 cggtatctcc cagcgaaggg tgaacccgtt gacgggttgc cagtcggttc cctgatcgga
    11941 acgcagattg tccgccggat ttgagaaccg gttgtcaatc aggacagtcc tggtggcgct
    12001 gcatgttttt gaagcgtttg cctcggagct cgcgtagtca cgagaacaga cccgaatctg
    12061 gtttgaccct tcctgaaagg gcgagaccga ggtatcgaaa gtcctgatcg tcgagtaagt
    12121 gagcgggcag ggagtgaacc tggtcgtgta gtcacccttg tcacccgggc aggtcggcgc
    12181 cgggaggtcg accctcagac cgttgatttc gacccaggtg gcagccactc ccgaaccctg
    12241 gtcggtggcg tcgatcccga tcgaggcagt accccggtga tagccggaat cgccacccca
    12301 gtcccagagg gttcccgagg ccgccgctgt cggcgcgacc gtgtccctga ccgtgaactc
    12361 ggtgtcggtt acctcgagga aggccttggt tccacccacc tggttcgcgc acccgttcac
    12421 cgattggcag agcagttcga tcaccaccat ccactgggga tgggctgggt cagaccagta
    12481 gctgacctga tctgaaccgt cgtgaggaat gccaccatcg atgtcggtat ggactccaac
    12541 gttgtagcca ttcagctggg cctggatgtt gttcttgtca ttgagccggg ccgcaatgcg
    12601 ggctccgatc acttcggttc cggtaggagc cgcccagacg aaatgaccgc cttcaccgta
    12661 ggcgagactg gtaccgctgc ggtcctggta gatgcccagc tttcccgaac ccgacgtgac
    12721 gcatccgctc acagcatcga aatgcgggct cgcattgatc gcggaatagg tgccttgaaa
    12781 atcgagaaaa ctggaaccgt cacattgcct catcacatac gtcccggcat tggctccgct
    12841 cgccccgacc aggcccaggg ccagtacccc gaggattacg acagccagtt tctggaatcc
    12901 gttcatggtg aaaactcccc tcctccgttt ccgacaggca ctgaacttga acttgaagct
    12961 ggttgagggg gaggtgctgc agcgggagcc ggcgttccgg ccgcctcggg tgtgaattcc
    13021 gtttcggtcg gagtcggtgc cggtgccgac tcggtgatca ccggcgcttc actgaccggc
    13081 tcgggggtgg tcgacaccgg ttcggcggcg gtagccgact gatccggaga tccaccggcc
    13141 tgtgattgcg gggccgtcga tggttccggc gcgggctcca cgaaatccag cttccgccgt
    13201 tcgggctctg gccgggctac cgatgcgtgc cgctcgacga ccggttttgc gtccttgtgg
    13261 ggcagcagat tcgcggccgg aagcaccccg gtggcggcac aaaccgccgc ggtacccgct
    13321 gtgccggcgc agatcgccgc gatcttggcg attgccgcca tcccggcacc acgagtacct
    13381 cccgaggcca ggaccgtctg aactccggag tccgaacccc ggccgactcc cgagaggcga
    13441 gtctgcaggt ccaccgcgag ttcctgggtt cgctcccaga gcccccggcc ggccggcaac
    13501 aggggagcca gcgctgcggc cgccctgggg gcagcgcgat aggcgcgaag ggttgaccga
    13561 cagtggccgc agacggcgag gtgggtttcc agctcggcaa cctgggatga ggtcaactcc
    13621 ccgtcgcagc tggccgagat cattggctcg aattcggggc acctctctcc cgcttcgctt
    13681 tcccggaagg tacttcggaa acgagcccgc ccttcggcca ggcaccggtt caccttggtg
    13741 cgggtccacg agttcatcga cccgatttcg tcgtatgaga atccctctgc gagttgagtc
    13801 agggccttca gttcctgggg cttcagctga gtcagggctt cccggctgcg ggccaccctc
    13861 tcgcggcgaa cggtgagttc atccgggccg tcgctgatat ccgggatgag ctgcacccag
    13921 tcgtcttcaa gatgctcgtc aatgtcggac tggcccgggc gggcgagcgt cctctcgcgg
    13981 gtccgccgaa tcgccaaagc ctcgtgcttg atgaccttcc tcgtccaggg caggagatgc
    14041 cgcgtgtcgg ttgtcggcgc tttcgagaga aggatctcga tgcctcgctg ataggcgtct
    14101 tcggcgtcct cgggacagat cgagtagcgg cgtgccgtcc acctgagcgg gcgatcgtga
    14161 tttctgatca actcgaccgc aacccgcttg cgcgctgcct catccggcct gatcccggcg
    14221 tcatgctctt ccgtgccttg aaccgcaact cccctcatgg ggtcccccac tccttagacg
    14281 gcttggcact cttcctatcc gggagacccg aagattgcca gcggcagaaa tcacacccac
    14341 ggaacctggc ccctacattt tcaaaccatc gaaaagcgtc catttgcagc gataagggcc
    14401 gcaggttcga aaactctccc cccttcaaag tccggtttct ccgcaaattg cgaactattc
    14461 gaaatttcat gcaaattgca tggaatttct gattcgtctc agttcgg
//
LOCUS       DAOQKN010000044        14409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_1952_length_14409_cov_6.333357, whole genome shotgun sequence.
ACCESSION   DAOQKN010000044 DAOQKN010000000
VERSION     DAOQKN010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 14409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14409
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1952_length_14409_cov_6.333357"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..1988
                     /locus_tag="PLJ64_04600"
     CDS             138..1988
                     /locus_tag="PLJ64_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92802.1"
                     /translation="MPRKFAQGLTAFGLAVAIIFGFTGNAAAAPADRSGDAVVLKGSE
                     IARLLGADPARLVGFSFRNGRWSQIPVQVDERHTVDVRSLYPPENRTYVGDSSPDFNL
                     EVYADPKTRSGADEDATFDANDELTFMGGDTGSRVPAGVGAPAPVVPGSGTAVRVSDP
                     VGGGNAWVYLFRSINRKFDPSAGKDYVDYDFKLTGLTGGQTLLNDYGYFSQFNPEDST
                     VKTDDYELHSYDRWMEDELKIRAGNANGTDILDREVAQAGRGSCGRSEYTFSGRWGQD
                     STSGNDNSTDNEGTYVAVIDGPVRAIRSYMGANSGPYVQREHIYYRDHEQNTIFLRVH
                     QMADLYTWTDFAPSAIGMTYRDAKNTAGVTVDGNPDSIAPTTSADISNGAYSWQQISG
                     PQGTATTVMGSDTDLTDPNFGNYYLDDSTPSGPLEVQCGGDGQSIGASGFGIFGEGDT
                     GITPNTDPRSAGFKTLTTRRTRYFGPPADGVAKAENYTARVSSPLTATSSPSPMKQGM
                     GSARAYILTRGVTAHRGRAIRLRVKVRNSGNFRIRKAKACVFGKGLTGRPCTRVNDLG
                     IGNERTVTLKTRVGRFAKGRKLKLSVSVTGSAGDWGFAGVGGYFLIPLRG"
     gene            2046..3866
                     /locus_tag="PLJ64_04605"
     CDS             2046..3866
                     /locus_tag="PLJ64_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92803.1"
                     /translation="MAVLGLAAAISTGIGGVASAAPADRGNDPVVLKGSQVAGLDGVA
                     PGRIVGFKWEGKWVQVPVQVDQRHTVSARGLYPDEAPAYVNNDLTFDVEIYADAKTRS
                     GADADPNLDPDDEIAFMGSDTGDAAPAATLSPKGVIGSTATRIDVVDPIDTSVAQVYL
                     FKSDGSLDPSAGKDYVDYDFNLTNLGPGETLIDDYGFSNSNNPEDSTVSTANYTLHST
                     DRWMEDEIKIKAGGASDTDILDREAASAGGLGGCGRSEYTFSGNWNRGSDNDEGTILA
                     IKDGPVRAIRDYMGANSGPYVEDTHVYYSDREDRMIHVRVHPIPLMYVWTDYNDQAVG
                     MTYRDQLNPTGVKIDGVPGEQDGQPFDDSIDKATPADVPSGQYLWQQVTGAQGTATTL
                     VSADSSVEPGGIFNGFAGYYLDDSTPTGSSERQCGGDMKAYGASGFGIDGVYPNTDPN
                     LATTASPAAKLSVNRIRYFTSPNGTAADADGLRDRAQDPLTGSSADANIKARAAKLKV
                     TLQGPAPKAKPGRQLRLKLKIANSGTADSAEGKVCVAGVALPHKTCRNFARIFRQKST
                     TVTLTPTVKKTVKANKLVLKVTATSGKASDGLTLKLPLDR"
     gene            complement(3877..4893)
                     /locus_tag="PLJ64_04610"
     CDS             complement(3877..4893)
                     /locus_tag="PLJ64_04610"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNC92804.1"
                     /translation="MASRREKKRTERTRAQMPRWMFETRTHQWHEAGLGEQIVGPPQG
                     RTWPRLILFAVLIAAVLVAFNHRQDIAPGYGTEARILTAILLFGLGWGFASALGRTVT
                     PFIMRRLDPGTAGTLGFVIRLGTILIVGLVALGIAGVKPQTLAVGGAATAIVLGLAAQ
                     QTLGNVFAGVVLQSTRPFRVGERVRLTGGPMAGSVEGTVSSLGLFYTTILKGQDRMMI
                     PNNLLLLLAVEPLREPDSIDLKARFDSHLSPATVQEMLRQAITVPTLRPPRIELEEVD
                     RDEVVLRISATPVNPSDGARLAEQVVSVTRGTFEYEKPDPEAIAREKGPDQKPDPDVP
                     HGHS"
     gene            complement(4976..7609)
                     /locus_tag="PLJ64_04615"
     CDS             complement(4976..7609)
                     /locus_tag="PLJ64_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92805.1"
                     /translation="MKRFNLLLIVAGLAAMLLAMPMSASAGKKPVDKATSGLKDTLKT
                     NVRIISTSQGPVRSSGQLKVKVSVKASKSTPRDIKKNPIKIALHGSAKVIWSPRSNVS
                     LYGETLKLKPNHSRVVKLKLNPTGKSKAGDCGAPTFEVTATYRSGKRKKTAKSSRKLA
                     PQKSLCDVPAGVDLASSDKCDFIAGPGNACLAPFPNDFYTREDEGSTTGKRLDLDPDS
                     TPANSSEGKHIAVNVLNQSDGFSPGPLLSVRIPGMDNQAAFDQSGIVSIKEMSAAYAP
                     AQPVVLIDAATGERQLIWAELDSQATTDADRNLIIRPAKNLTNGHRYIVAIRNMKDSS
                     GDPIQAPAAFRLYRDTDRTGDNRIESRRAHFESIFKTLGDAGIQRKSLYLAWDFTVAS
                     TENITGRMLSIRDRAFAGLGDTNLTDGVVQGDAPAYHIDSVTDYTGNHTGAGTEDVRV
                     VKGTVTVPCFLNQAGCPSGSTFDLDSDQMPIRSEGNTMQARFSCNIPRSVVGDGAGGG
                     DDTQFHQTARPSLYGHGLFGSINEVTSRNIRQLGVENNVLVCGSDWVGMADQDIGSAI
                     NALLDLSKFPALPDRLQQGFLNFLFLGRLMIHPDGFAADPAFKVDGHSVIDTSDLFYY
                     GNSQGGIAGGALTAVATDFTRSVLYVPGMNYSMLLTRSTDFEDYKSILYPAYQNELER
                     PSIFALMQSMWDRGEPNGYANNMTSNPLPNTPAHHVMIEMSYGDHQVANVATEVEART
                     IGAPLRTPTIDADRAATGLIEPFYGHDTLGDLAGPAADGNAYFVWDIGPQRLQEDPEG
                     SGNMKLFGTDPAPLTNTPPVTGESDTPLGETSGIDPHDTVIEESPLIRKQIADFLKTD
                     GKVTNPCGLNPCYAAKWKGMP"
     gene            7874..10369
                     /locus_tag="PLJ64_04620"
     CDS             7874..10369
                     /locus_tag="PLJ64_04620"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNC92806.1"
                     /translation="MAFGVAAGQAQAKPSIAAQAKKICKKKRGKAKAKCVKKQVKRLK
                     AKARRERLKELNRPGVTIRTTEYGIPRITADSWRGLGYGYGYALANENICSMADIYTT
                     VRGERSKYFGPTGTWMLTGNGIQYTNLESDFSHKLVIAQNTIPKILKMKPPNGPLPQV
                     REVIDGYVKGYNAWLTKTGVNKIQDSTCKGKPWVKKITPLDAYLRFYELATMAGSGAA
                     VDGIANAAPPAETRTGAPSGSGDSNVTAADFKALNKPAAIGSNAVSLGSQATDTGKGM
                     LFGNPHFPWSGSERFFESQLTIPGKINVSGASLLGSPVIQIGHTQNMAWSHTVSTARR
                     FAFFRESLDPSDPTRYMVDGVSTPMKSNTITVDKGNGDTETRTLYSTKHGQISTSIQK
                     TPLFGWDSNYAYALFDANANSLRVVNQFFSFNTAESVAGMKKALETYQGVPWVNTIAA
                     DSKGNAMYADVSVVPNLTDARVAECNTPGLGEVAWGSSKVAVLDGANSDCDMQKAPGA
                     VVTGVLPPSQMPLAIRKDYGSNMNDSYWLSNPNAPLEGFPSIIGNEQSARSLRTRNGL
                     HQIETRLDGTDGDPGNKFSIDRVRQFTTNNRNYGAQVLIPGMVNYCESHPTIDSVDVS
                     AACTVLDQWDWTENLDSPGAYLGREVIGNLLAVSGGPFADPFSLSDPVNTPSGLDTSK
                     PGVPLALSAAVTYMNSKSIPLDASWRDYQYVTKNGEQIPIPGGFGGQGMFNVTSAVRN
                     SSTGVYDSVRHGSSFIITASLTGAKCPDVKTILTYSQAATNELSPHYADQTKLYSNSG
                     WVTDRFCVAQQKSSPGLKVTSLNGGAKAVKKGW"
     gene            10486..11328
                     /locus_tag="PLJ64_04625"
     CDS             10486..11328
                     /locus_tag="PLJ64_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNC92807.1"
                     /translation="MRAAVVQINSGADRSRNLEVAGELVVAAAADGAELVVLPEKWAL
                     LGDGAALTAGAESLDGEAITAARGWAADLGIHLLAGSFTMLPDPGGVPPSALPTNTSV
                     LISPEGQIVCSYDKLHMFDVEAGGVEYRESDHEQAGDGLAVADAGDLRIGLTICYDLR
                     FPELFRALLDRGATAFTVPSAFTSATGRDHWETLLRARAIENQAFVLAANQVGTAAPS
                     YDSWGHSMIVGPWGEVLAEVKEGEGFACAELDLERQAEVRRKLPAVNHRRPEIFSEGV
                     AHGS"
     gene            11318..11917
                     /locus_tag="PLJ64_04630"
     CDS             11318..11917
                     /locus_tag="PLJ64_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC92808.1"
                     /translation="MAREAAPERRRQILDAAVRVFARQGFHSTRVADIADEADVAYGL
                     VYHYFSSKENVLNELFIQRWSLLIEAIDEADRSTLAHEEKLGAVAGFIIDSYRYDPDL
                     MKVIIVEVTRAANSFGQTHLEEINRAYELIGRIVADGQKQSIFREDIDPAFAAMVFYG
                     AIEQLLSGWIFGVIPGSDRDFDEARDLLVKAICEGLERR"
     gene            12069..12203
                     /locus_tag="PLJ64_04635"
     CDS             12069..12203
                     /locus_tag="PLJ64_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92809.1"
                     /translation="MDNDIVRRLTWSVMLTASSALASLIAARLAAVAYRRFFNEDPPE
                     "
     gene            12300..12674
                     /locus_tag="PLJ64_04640"
     CDS             12300..12674
                     /locus_tag="PLJ64_04640"
                     /inference="COORDINATES: protein motif:HMM:NF018980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNC92810.1"
                     /translation="MVFEVSERTSNLIREEIELAKTEVSEKVTTLARGSAVGIAAGVF
                     AFLALILLMHAFALGVASLFGWGAWAGYLLEGVIFIAIAGGAGYFAYSSFEKTGAPIP
                     TEAIEEAQKTKALLTPGEDSNE"
     gene            12667..13062
                     /locus_tag="PLJ64_04645"
     CDS             12667..13062
                     /locus_tag="PLJ64_04645"
                     /inference="COORDINATES: protein motif:HMM:NF023697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HNC92811.1"
                     /translation="MSDEKPETGASGSENPVEGVEKPFRPEGVGPEAPVDRTPNRHVA
                     PEGTPAPAAGAAGSPYSPPPPKRNSTQIRADIDAQRAELGKSVDQLRDRVTELTDWKS
                     QLRKHRKQIIVGAVATGFVVGGLMALRRR"
     gene            complement(13568..14089)
                     /gene="def"
                     /locus_tag="PLJ64_04650"
     CDS             complement(13568..14089)
                     /gene="def"
                     /locus_tag="PLJ64_04650"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNC92812.1"
                     /translation="MAVREVLTYPDPILKSVCSPAQSGPDDREETGRVITDLLDTMNS
                     FDHCVGLAAPQIAESVRIAVVDITGHPRAKEPHGLMVLVNPRVVHRSEGTKVSREGCL
                     SLPDLTANVRRPRKATVEFLDGQRNLEMNQVQLAGFEARCVLHEIDHLDGILFLDRVA
                     SITDDLFRRQNYG"
     gene            complement(14123..>14409)
                     /locus_tag="PLJ64_04655"
     CDS             complement(14123..>14409)
                     /locus_tag="PLJ64_04655"
                     /inference="COORDINATES: protein motif:HMM:NF025116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNC92813.1"
                     /translation="GLDRGAGPTHVLTVYGADRPGIVHDTAATLASLGVNITDLRTRR
                     TGDPDSPLYTLMVEVAVPEGTGPELDQALDGLRQSKQLEARLAELESEVF"
BASE COUNT         2608 a         4717 c         4663 g         2421 t
ORIGIN      
        1 cagccagcga aaaaggccgc gcggaccagg gtgatgagga tcggcaagga tgtcggcaga
       61 tttcagaagt cggttgcccg caactgcggc tgacctggtc tgttcaaatc accgtcgacc
      121 aacgatcaag gagaaacgtg cccaggaagt tcgcgcaggg actgactgcc ttcggcctgg
      181 ccgtggcaat cattttcgga ttcaccggca acgccgccgc ggcaccggcg gaccgctccg
      241 gagatgccgt cgtgctcaag ggcagtgaaa tcgccaggct gctcggggcc gacccggcgc
      301 gcctggtcgg cttctccttc aggaacggtc gctggagcca gatcccggtg caggttgacg
      361 agcgccacac ggttgacgtg cgcagccttt acccgcccga gaaccgcacc tacgtgggag
      421 acagcagccc ggatttcaat ctcgaggtct acgccgaccc caagaccagg tcgggcgccg
      481 acgaggacgc aacctttgac gccaacgacg agctgacctt catgggcggt gacaccggca
      541 gccgggttcc ggccggcgtt ggtgctcccg ccccggttgt tccgggctcg ggaacggcgg
      601 tcagggtgag cgacccggtc ggtggcggca acgcctgggt ctatctcttc cgatcgatca
      661 accggaagtt cgacccttcg gccggcaagg actacgtcga ctacgacttc aagctgaccg
      721 gactgaccgg gggacagacc ctgctgaacg actacggcta cttcagccag ttcaaccccg
      781 aggactcgac cgtcaagacc gacgactacg agctccactc gtacgaccgc tggatggaag
      841 acgaactgaa gatccgggcc ggcaacgcga acggaaccga catcctcgac cgggaagtcg
      901 cgcaggccgg acggggcagc tgcggccgct ccgaatacac gttctccggc cgctggggcc
      961 aggattcgac cagcgggaac gacaactcga ccgataacga gggcacctat gtcgccgtga
     1021 tcgacgggcc ggtacgggcg atccgctcct acatgggcgc caattccggg ccttacgtcc
     1081 agcgcgagca catctactac cgggaccacg agcagaacac gatcttcctc cgggttcacc
     1141 agatggccga tctctacacc tggaccgatt tcgcgccttc ggcgatcggc atgacctacc
     1201 gcgacgcgaa gaacacggcc ggcgtcacgg tcgacggcaa tccggattcg atcgccccga
     1261 ccaccagcgc cgacatctcg aacggggcct actcgtggca gcagatctcc ggcccccagg
     1321 gaacggcaac cacggtgatg ggatcggata ccgatctcac ggacccgaac ttcggcaact
     1381 actacctcga cgattcgact ccgtccggtc cgctcgaggt ccagtgcggg ggtgacggcc
     1441 agtcgatcgg agccagcggc ttcgggatct tcggcgaggg cgacacgggc ataaccccga
     1501 acacggaccc ccgcagcgcc ggtttcaaga ctctgacgac caggcgcacc cgttatttcg
     1561 gcccgccggc tgacggggtc gccaaagcgg agaattacac ggccagggtg agcagcccgc
     1621 tgaccgcgac cagcagcccg agcccgatga agcagggcat gggttcggcc cgcgcctaca
     1681 tcctcacccg gggcgtaacc gcgcaccgtg gccgggcgat caggctccgg gtcaaggtac
     1741 gaaacagcgg caacttccgg atccggaaag cgaaggcctg tgttttcggc aagggactga
     1801 ccggcaggcc atgcacccgg gtcaacgatc tcggtatcgg caacgagcgg accgtgactt
     1861 tgaagaccag ggtcggccgg ttcgcgaagg gcaggaaact gaagctgagc gtctcggtca
     1921 ccggcagcgc gggtgactgg ggatttgccg gcgtcggcgg ttacttcctg attccactcc
     1981 gcggctgagc ggctatcctt cccgacatgg gatgggacct cgcgcgtaag acgttcgcgc
     2041 ggtcgatggc tgtgcttggc ctcgccgcgg caatttcaac cgggatcggt ggtgtcgcgt
     2101 cggcggctcc ggccgaccgg ggcaatgatc cggtcgttct gaagggcagc caggtcgcgg
     2161 ggctcgacgg tgtcgcgccg ggccggatcg tcggtttcaa atgggaagga aagtgggtcc
     2221 aggttccggt tcaggtggac cagcgccaca cggtcagtgc ccgcggcctc tatcccgacg
     2281 aggcgcccgc ctacgtcaac aacgacctga ccttcgacgt cgagatttac gccgacgcca
     2341 agacccgctc cggcgccgac gcagacccca atctcgaccc ggatgacgag atcgccttca
     2401 tgggcagtga caccggagat gccgctccgg ccgccacgct ttcgccgaaa ggcgtcatcg
     2461 gttccaccgc gacgcggatc gatgtggtcg atccgatcga caccagcgtc gcccaggtct
     2521 acctcttcaa gtccgacggt tcactcgacc cctcggccgg gaaggactat gtcgattacg
     2581 acttcaacct gaccaacctc gggccgggcg agaccctgat cgatgactac gggttctcga
     2641 actcgaacaa tcccgaagat tcgacggtca gcacagccaa ctacaccctc cactccaccg
     2701 accgctggat ggaagacgaa atcaaaatca aggcgggtgg cgcgagcgat accgacattc
     2761 tcgaccgcga ggcggcctcg gccggtggcc tcggtggctg cggccggagt gaatacacct
     2821 tctccggaaa ctggaatcgc ggcagcgaca acgacgaagg cacgattctc gcgatcaagg
     2881 acggcccggt ccgcgcgatc cgggactaca tgggcgccaa ctccggcccg tatgtggagg
     2941 acacccatgt ctactactcc gatcgcgaag accggatgat ccacgtgcgg gtccacccga
     3001 tcccgctcat gtatgtctgg accgactaca acgaccaggc ggtaggcatg acctatcgtg
     3061 accagctgaa cccgactggc gtcaagatcg acggcgttcc cggcgagcag gatggtcagc
     3121 ccttcgacga ttcgatcgac aaggcgacgc cggccgacgt gcccagcggc cagtacctct
     3181 ggcagcaggt gaccggcgcg caaggcaccg cgaccactct ggtctcggcg gacagctcgg
     3241 tcgaacccgg cggcatcttc aacggattcg ccggttacta cctcgacgat tcgactccca
     3301 ccggctcctc cgagaggcag tgcggcggtg acatgaaggc gtacggggcc agcggctttg
     3361 gcatcgacgg cgtctacccg aacaccgatc cgaaccttgc caccaccgct tcaccggcgg
     3421 cgaagctctc ggtcaaccgg atccgctact tcaccagccc gaacggcacc gccgccgacg
     3481 cagacggcct ccgggaccgc gcccaggacc cgctcaccgg ttcgtcggca gacgccaaca
     3541 tcaaggcccg ggcagccaag ctgaaggtca cgcttcaggg tcccgctccg aaggcgaagc
     3601 cgggcaggca actcaggctg aaactgaaga tcgcaaacag cggtaccgcg gactcggctg
     3661 aaggcaaggt ctgtgtggcc ggtgtggccc tcccgcacaa gacctgcagg aacttcgcgc
     3721 ggatcttccg ccagaagtcg accacggtga ccctcacacc gaccgtgaag aagaccgtga
     3781 aggcgaacaa gctggtcctc aaggtcactg ccacttcagg caaggcctcg gatggcctga
     3841 cgctgaagct ccccctcgac cgctaggaag tcccgctcag gaatgaccgt ggggaacatc
     3901 ggggtcgggc ttctggtccg gccccttttc cctcgcgatc gcttccggat ccggcttctc
     3961 gtattcgaag gtgccgcggg ttaccgagac gacctgctcg gccagcctgg cgccgtctga
     4021 cgggttgacc ggggtggcgc tgattcgaag caccacttcg tccctgtcga cctcctccag
     4081 ctcgatgcgg ggaggccgca gggtcggcac ggtgatcgcc tggcgcagca tctcctgaac
     4141 ggttgccggg ctcaggtggc tgtcgaagcg ggccttcaga tcaatcgaat ccggttcgcg
     4201 cagcggctcg accgccagca gcaggagcag gttgttggga atcatcatcc ggtcctggcc
     4261 cttgaggatc gtcgtgtaga agaggccgag cgaactgacc gttccctcga ccgagccggc
     4321 catcggcccg ccggtcaggc gcacccgctc gccgacccgg aacggccggg tgctctggag
     4381 cacgaccccg gcaaagacgt tgccaagggt ctgctgggcg gcgagaccga ggacgatcgc
     4441 ggtggccgca ccgccgaccg ccagggtttg cggtttgacc ccggcaatcc cgagcgcgac
     4501 cagaccgacg atcaggatcg tgcccagccg gatgacgaag ccgagtgtgc cggccgttcc
     4561 cgggtcgagc cggcgcatga tgaagggagt gaccgtgcgg ccgagggcgg aggcgaaacc
     4621 ccagcccagg ccaaagagca ggatcgcagt gaggatcctc gcctcggtgc cgtagcccgg
     4681 ggcgatgtcc tgccggtgat tgaaggcgac caggaccgcc gcgatcagca cggcgaagag
     4741 gatcagcctc ggccaggtcc gtccctgtgg cgggccgacg atctgttcgc caaggccggc
     4801 ttcatgccac tggtgggtcc gggtctcgaa catccacctc ggcatctggg cccgggtgcg
     4861 ttcggtcctt ttcttctcac gtcgtgaagc cacgacggtt caccatacaa agagcccggg
     4921 ttgaagctgc ccgcagatca ggcgaacggc tccaacccgg gcgggaactt ccggcctacg
     4981 gcatgccctt ccacttggcg gcgtagcagg ggttgagacc acaggggttt gtgaccttgc
     5041 cgtccgtctt gaggaagtcg gcgatctgct tgcggatcag cggtgactct tcgatcaccg
     5101 tgtcatgcgg gtcgatgccg ctggtctcgc cgagcggggt gtcggactcg ccggtgaccg
     5161 gcggggtatt ggtcagcggt gccggatcgg ttccgaacag cttcatgttg ccgctgcctt
     5221 ccggatcttc ctgcagccgc tgggggccga tgtcccagac gaaataggcg ttgccgtcgg
     5281 cagccgggcc ggccaggtca cccagggtgt cgtgaccgta gaacggctcg atcaggcccg
     5341 tggcggcccg gtccgcgtca atcgtcggcg tcctcagcgg agcgccgatc gtccgggcct
     5401 cgacctcggt tgcgacgttc gccacctgat ggtcgccgta ggacatctcg atcatcacgt
     5461 ggtgggccgg ggtgttcggc aacggattgc tggtcatgtt gttggcgtag ccatttggct
     5521 cgccgcggtc ccacatcgac tgcatcaggg cgaagatcga gggtcgctcg agctcgttct
     5581 ggtaggccgg gtagaggatc gacttgtagt cctcgaagtc ggtgctgcga gtcagcagca
     5641 tcgagtagtt catacccggc acgtagagca ccgacctggt gaaatcggtc gccaccgcgg
     5701 tcagggcacc gccggcgatt ccgccctggc tgttgccgta gtagaagagg tcactggtgt
     5761 cgatcaccga gtgtccgtcg accttgaagg ccgggtcggc cgcgaagccg tccgggtgga
     5821 tcatcagccg gcccaggaac aggaagttga ggaaaccctg ctgcaggcga tccggcaggg
     5881 ccgggaactt gctcaggtcg agcagcgcgt tgatcgcgga tccaatgtcc tggtcggcca
     5941 tcccgaccca gtcggagccg cagaccagga cgttgttctc gacgccgagc tggcggatgt
     6001 tgcggctggt cacctcattg atcgagccga acagaccgtg accgtaaagc gaggggcggg
     6061 ccgtctggtg gaactgggtg tcatcgccac cgccggcacc gtcgccgacc accgagcgcg
     6121 ggatgttgca ggaaaaccgc gcctgcatcg tgttcccttc gctgcggatc ggcatctggt
     6181 cggaatcgag atcaaacgtg gaaccggacg ggcaaccggc ctggttcagg aagcagggca
     6241 ccgtgacggt gcccttgacc acccggacgt cctcggtgcc ggcgccagtg tgattgcccg
     6301 tgtagtcggt taccgaatcg atgtggtagg ccggggcatc gccctggacc acgccgtcgg
     6361 tcaggttggt gtccccgagc ccggcgaagg cccggtcccg gattgaaagc atgcggccgg
     6421 tgatgttctc ggtgctcgcc accgtgaagt cccaggccag gtagagcgac ttccgctgga
     6481 ttccggcatc gccgagggtc ttgaagatcg actcgaagtg ggcccggcgg ctctcgatcc
     6541 tgttgtcgcc ggtgcggtcg gtgtcgcggt acagccggaa cgcggccgga gcctggatcg
     6601 ggtcaccgga ggaatccttc atgttgcgga tcgcgacgat gtaccggtgg ccgttcgtca
     6661 ggttcttcgc gggccggatg atcagattgc gatccgcatc cgtggtcgcc tgactgtcga
     6721 gctcggccca gatcagctgg cgctcgccgg tcgccgcgtc gatcagcaca accggctggg
     6781 cgggggcgta ggcggcgctc atctccttga tcgaaacgat cccggactgg tcgaaggcgg
     6841 cctggttgtc catgccgggg atccgcaccg aaagcagcgg accggggctg aacccgtcgg
     6901 actgattcaa cacgttcacg gcgatgtgct tgccctcgga ggagttcgcc ggcgtggaat
     6961 cggggtcgag gtcgagccgc tttccggtgg tcgagccctc gtcctccctg gtgtagaagt
     7021 cgttcgggaa gggggccaga caggcattgc ccggtccggc gatgaagtca cacttgtcgg
     7081 aactggcgag gtcaacgcca gccggcacgt cgcagagcga cttctggggg gccagcttgc
     7141 ggctggactt tgcggtcttc ttgcgcttgc cggaacggta ggtcgcggtc acctcgaagg
     7201 tcggggcccc gcaatcgccg gccttggact tgccggtcgg gttcagcttc agtttcacca
     7261 ccctggagtg gttgggcttg agcttcagcg tctcgccgta cagcgagacg ttcgaacggg
     7321 gggaccagat gaccttggcg ctgccatgaa gcgcgatctt gatcgggttc ttcttgatgt
     7381 cccgaggcgt cgatttcgaa gctttgaccg agaccttgac cttgagctgg ccgctcgagc
     7441 ggaccggtcc ctggctggta ctgatgatcc ggacgttggt cttgagcgtg tccttgagac
     7501 cgctggtcgc cttgtccacc ggcttcttcc cggccgacgc ggacatcggc atcgccagca
     7561 gcatcgccgc cagtcccgcc acgatcaaca gaagattgaa tcgcttcacc tgactcccct
     7621 tttccctctc ggttttctcc ccgggtcgaa tgaccctttc cgaagaatag cgccgatcgg
     7681 gcgacaaacc gggaaacgga agcggtttcg ggaaagcggc cgtaccgtac gcggatgtat
     7741 gcttggaccg ctccgaagct ctgttttcgg ggttagaggg attggagaaa tgaggggccg
     7801 caggggcgtc tcgtcaaaga ctggagagaa cttgaagtcc atcagacttt tggttgcagc
     7861 cattgtcatc ttgatggcat tcggtgttgc agcaggacag gcacaggcca agccgagcat
     7921 tgcggcgcag gcgaagaaga tctgcaagaa gaagcgcggc aaggcgaagg ccaagtgcgt
     7981 gaagaagcag gtcaagcggc tcaaggcgaa ggccaggcgg gaacggctca aggagctgaa
     8041 ccgccccggc gtcacgatcc ggaccaccga gtacgggatt ccgcgcatca ccgctgacag
     8101 ctggcgcggc ctcggttatg gctacggcta cgccctggcc aacgagaaca tctgctcgat
     8161 ggcggatatc tacaccacgg tgcgcggcga gcgctcgaag tacttcggcc cgaccggcac
     8221 ctggatgctg accggaaacg ggattcagta cacgaacctc gagtcggatt tctcgcacaa
     8281 gctggtcatc gcccagaaca cgatccccaa aatcctgaag atgaagccgc cgaatggtcc
     8341 gctgccgcag gtcagggaag tgatcgacgg ttacgtcaag ggctacaacg cgtggctgac
     8401 caagaccggc gtcaacaaga tccaggacag cacctgcaag ggcaagccgt gggtgaagaa
     8461 gatcaccccg ctcgacgcct acctgcggtt ctacgagctc gccaccatgg ccggctccgg
     8521 cgccgcggtc gacggcatcg ccaatgcggc cccgccggcc gagacgcgta ccggcgcccc
     8581 gtccggttcc ggtgactcca acgtcaccgc cgccgacttc aaggcgctca acaagcccgc
     8641 cgcgatcggc tcgaacgccg tttcgctggg ttcgcaggca accgataccg gcaagggcat
     8701 gctcttcggc aacccccact tcccgtggtc cggttccgag cggttcttcg aatcgcagct
     8761 gaccatcccc ggcaagatca acgtctccgg cgccagcctg ctcggcagcc cggtgatcca
     8821 gatcggccac acgcagaaca tggcctggag ccacacggtc tcgaccgcgc ggcgcttcgc
     8881 cttcttccgc gagtcgctgg acccgagtga tccgaccagg tacatggtcg acggggtttc
     8941 gaccccgatg aagtcgaaca cgatcacggt tgacaagggc aacggggaca ccgagacccg
     9001 caccctctac tcgaccaagc acggacagat ctcgacttcg atccagaaga ccccgctgtt
     9061 cggctgggac agcaactacg cctacgcgct gttcgacgcg aatgcgaaca gccttcgggt
     9121 cgtcaaccag ttcttcagtt tcaacactgc ggaaagcgtt gccggcatga agaaggcgct
     9181 ggagacctac cagggcgtgc cgtgggtcaa cacgatcgcg gccgactcca agggcaacgc
     9241 gatgtacgcc gacgtttcgg tcgtgccgaa cctgaccgac gcgcgggttg cggaatgcaa
     9301 cacgcctggc ctgggtgaag tcgcctgggg cagctcgaag gtcgcggtgc tggatggcgc
     9361 gaattcggac tgcgacatgc agaaggcacc cggagccgta gtgaccggtg tcctgccgcc
     9421 gagccagatg ccgctggcga tccgcaagga ctacgggtcg aacatgaacg acagttactg
     9481 gctgtcgaac ccgaacgctc cgctggaggg tttcccgagc atcatcggca acgagcagtc
     9541 ggcccggtcg ctgaggaccc ggaacggcct gcatcagatc gagaccaggc tcgacggcac
     9601 cgacggtgac ccgggcaaca agttctcgat cgaccgcgtg cgtcagttca ccacgaacaa
     9661 ccggaactac ggcgcccagg tgctgattcc gggcatggtc aactactgcg agagccaccc
     9721 gacgatcgac tcggtcgatg tgtcggcagc ctgcacggtg ctcgaccagt gggactggac
     9781 cgagaacctc gattcgcccg gggcctacct cggtcgcgag gtgatcggca acctgctcgc
     9841 ggtttccggc ggtccgttcg cggatccgtt cagcctgagc gacccggtca ataccccgag
     9901 tggtctcgac acgtccaagc cgggcgtgcc gctggccctc tcggctgcgg tcacttacat
     9961 gaacagcaag agcatcccgc tcgacgcttc ctggcgcgac taccagtacg tgaccaagaa
    10021 cggtgagcag atcccgatcc ccggcggttt cggcggtcag ggcatgttca acgtgacctc
    10081 cgcggtgagg aactcatcca ccggcgtgta cgacagtgtg cgccacggct cgagcttcat
    10141 catcacggcg tcgctgaccg gcgcgaagtg tccggacgtg aagacgatcc tgacctactc
    10201 gcaggccgcc acgaacgagc tgtccccgca ttacgcggac cagaccaagc tctactcgaa
    10261 cagcggctgg gtcaccgatc gcttctgcgt cgcccagcag aagagctcac ccggcctcaa
    10321 ggtcaccagc ctcaacggtg gtgccaaggc cgtgaagaag ggctggtagg ggagcacccg
    10381 ccacacccgc tgtattcgga gcgcccggcc ttgaggccgg gcgctccggt ttcttaatcg
    10441 acctatactt tccttcttga ttgacgtgtc aatcaagtcc cgatcatgag agctgccgtc
    10501 gtacagatca actcaggtgc cgaccgctcc cgcaacctcg aagttgccgg cgaactggtt
    10561 gtcgccgccg cagctgacgg cgcagagctg gtcgtgctgc cagagaaatg ggcactgctc
    10621 ggggatggcg ctgctctcac cgccggggcc gagtcgctcg acggagaggc gatcacggcc
    10681 gcccgtggct gggccgcgga tctcggcatc cacctgctgg cgggcagctt cacgatgctc
    10741 cccgaccccg gtggcgttcc cccttcggct ctgccgacca acacctcggt cctgatctcc
    10801 cccgaagggc agatcgtgtg ctcctacgac aagctccaca tgttcgacgt cgaagcgggc
    10861 ggagtggagt accgcgagtc cgaccacgag caggccggag atggtctggc ggtggccgac
    10921 gccggcgatc tcagaatcgg cctgaccatc tgttacgacc tgcgctttcc cgaactcttc
    10981 cgggccctgc tcgatcgcgg cgccaccgcg ttcacggtgc cgtcggcctt caccagcgcc
    11041 accggcaggg accactggga gacgctgctc agggcccggg cgatcgagaa ccaggcattc
    11101 gttctggcgg ccaaccaggt tggtaccgcc gcgccttcgt acgactcctg ggggcattcg
    11161 atgatcgtcg gtccctgggg cgaggtgctg gccgaagtga aggaggggga ggggttcgcc
    11221 tgtgccgaac tcgatctcga aaggcaggcg gaggtccgcc gcaagctgcc ggcggtaaac
    11281 catcgccggc cggaaatttt cagtgaagga gtcgctcatg gctcgtgagg ccgcgccgga
    11341 acgccggcgc cagatcctcg acgcggcggt ccgcgtcttc gcccgtcagg gcttccactc
    11401 cacccgggtg gccgacatcg ccgatgaggc cgatgtcgcc tacggcctgg tgtaccacta
    11461 cttctcctcc aaggagaacg tcctcaacga gctcttcatc cagcgctggt cgcttctgat
    11521 cgaggcgatc gatgaggccg accgcagcac cctcgcccac gaggagaagc tgggcgcggt
    11581 ggccggtttc atcatcgact cctaccgcta tgacccggac ctgatgaagg tgatcatcgt
    11641 cgaggtcacg cgggcggcca attccttcgg ccagacgcac ctggaggaga tcaaccgcgc
    11701 ttacgagctg atcggccgga tcgtcgccga cggccagaaa cagagcatct tccgcgagga
    11761 catcgacccg gccttcgccg ccatggtctt ctacggcgcg atcgagcagc tcctctccgg
    11821 ctggatattc ggagtgatcc ccggcagcga ccgtgacttc gacgaagccc gggacctcct
    11881 ggtcaaggcg atctgcgaag gcctggaacg ccgctgacgc ccgggcggcg ccgggaaccc
    11941 ccgaccgagg gtttgttcct gcctgtcttg ttgaacctca ggtagccacg ggatcgcccg
    12001 tgatcctccg accccgcgga ccgtttccgt attcgttctc cggaggcttg ggtattgtgg
    12061 tttgaccaat ggataacgac atcgtcagaa gactcacctg gagcgtcatg ctcaccgcga
    12121 gcagcgcctt ggcgagcctg atcgccgccc ggcttgctgc ggttgcctac cgccggttct
    12181 tcaacgagga cccgcccgaa tagaccgtgg ctccagagga ctaccagccg ccgccctacc
    12241 agcgaccacc cggccgcccg gatccgaatc gcccggcatc cgaaaagacc gtcggggaaa
    12301 tggtcttcga ggtttccgag cgcacctcga acctgatccg tgaagagatc gagctcgcca
    12361 agaccgaggt cagcgagaag gtcaccaccc tggcccgcgg ctcggcggtc ggcatcgccg
    12421 ccggagtctt cgccttcctc gcactgatcc tgctgatgca cgccttcgcg ctcggcgtcg
    12481 cctcgctctt cggctggggc gcctgggccg gctacctgct ggaaggcgtg atcttcatcg
    12541 cgatcgccgg cggcgccggg tatttcgcct acagctcttt cgagaagacc ggcgccccga
    12601 tcccgaccga ggcgatcgag gaagcccaga agaccaaggc cctgctgacc cccggagaag
    12661 attccaatga gtgacgaaaa gcccgagacg ggcgcatccg gatcggagaa tccggtcgaa
    12721 ggtgtcgaga agcctttccg tcccgagggc gtcggcccgg aggccccggt cgaccgcaca
    12781 ccgaaccgcc atgtcgctcc cgagggcacc ccggccccgg ccgccggcgc ggcaggctcg
    12841 ccctactcgc caccgcctcc gaagcggaac tcgacccaga tccgggcgga catcgacgcc
    12901 cagagggccg agctcggcaa gtccgtggac cagctccgcg accgggtgac cgagctcacc
    12961 gactggaaga gccagctccg caagcaccgc aagcagatca tcgtcggcgc ggtcgccacc
    13021 ggctttgtag tcggcggact aatggccctc cgccgccggt agacagcccc cgggcaccgc
    13081 gcccaggaac caccagccgt ccgctagctt cgtcgtcggc cggaaacgac agctcatgta
    13141 cccgcgtact atcgcggtca tttccggcct gtctccttgc cagcgaacgg ctggaggccc
    13201 ctggcatcca cccgctcacg gctggaggag accacgaccc cgctcacggc tggtggtgtt
    13261 ggggcatttc gcgagatgcg gtttgtcggc atatatcgcc gacaaacagc atctcgatcg
    13321 attcctgacc gtgactcggt ggccggtgac gcaggtttgc gacctcagag gttgcaaacc
    13381 tgcggcacca gcctggatag ggacgcgacc tggccgtgaa gccagctcgg acccgggatc
    13441 ccttcaactt cccccttcac ctgccctgtt ccaccgcagt gggtcggatt tccgtcgtag
    13501 cagccagaaa accgacccac cggaccatcc gaaccggtcc tcgacagcgc agtgggtcgg
    13561 ttgcgggtta gccgtagttc tggcggcgga agagatcgtc ggtgattgag gcgacgcggt
    13621 cgaggaagag gattccgtcg aggtggtcga tctcgtggag gacgcagcgg gcctcgaagc
    13681 cggccagctg gacctggttc atctcgaggt tcctctggcc gtccagaaac tcgaccgtgg
    13741 cctttcgggg gcggcgaacg ttggctgtga ggtcgggcag cgagagacag ccctcgcggc
    13801 tgaccttcgt tccttccgag cggtgaacca cccgggggtt gaccagcacc atcagcccgt
    13861 gcggttcttt cgccctggga tggccggtga tgtcgaccac ggcgatccgg accgactccg
    13921 cgatctgggg cgcggccagg ccgacgcagt gatcgaagga attcatcgtg tcgagcaggt
    13981 cggtgatcac ccgcccggtt tcttcgcggt catccggtcc cgactgggca ggggaacaga
    14041 ccgacttgag aatggggtcg ggataggtga ggacctcgcg aactgccaac ggttgcctgc
    14101 ctcagaccgc ggagccggcc acttagaaga cctcggactc gagttcggcg agccgggctt
    14161 ccagctgctt cgactggcgc agcccgtcga gggcctggtc gagttccgga ccggttcctt
    14221 ccggcaccgc tacctcgacc atcagcgtgt agaggggcga atcgggatcg cccgtgcgcc
    14281 gggttcgcaa gtcggtgatg ttgacgccga gtgaagccag cgtggccgcc gtgtcatgga
    14341 cgatcccggg acggtcggcc ccgtagaccg tgagtacatg ggtcggtccc gctccacggt
    14401 ccagcccct
//
LOCUS       DAOQKN010000045        14324 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_1974_length_14324_cov_6.262737, whole genome shotgun sequence.
ACCESSION   DAOQKN010000045 DAOQKN010000000
VERSION     DAOQKN010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 14324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14324
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1974_length_14324_cov_6.262737"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..695)
                     /locus_tag="PLJ64_04660"
     CDS             complement(105..695)
                     /locus_tag="PLJ64_04660"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNC92814.1"
                     /translation="MSDQRGLKMWWQKRRWYERSHRPLSRFRLDLEMARRGSYVRHPI
                     EGDVLGALRDGRLEIGQGCHFEPGCWLTMSDHARVTLGDDVWLNRNVMLAAENEVTIG
                     DHVMFANGCFVGDASHRFNDPTIPITWQGFTSKGPVRIGSNVWLGVNCVVTSGVTIGD
                     HCVIGANSVVTTDLPDNTISAGSPAKVIKEVEFLDH"
     gene            756..2471
                     /locus_tag="PLJ64_04665"
     CDS             756..2471
                     /locus_tag="PLJ64_04665"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNC92815.1"
                     /translation="MLRRKVDQPEDQAAETGAAEAAEAAGTAPETEPVPESAVTGSES
                     ASNEPEPAQPEPGSASPEETAAAGTEPGSAPAEVEPAGVLEEDVRDEQIEVTPGPVAS
                     HSPEPQTPAEQLTARQQSVHQAPEPDSKDFMKDRREASKAQRKADKEKRKADRKAAKE
                     ARKARRQSVGQSEAMALDPLAPTTPGPVAPPRPPNPPVGSPAAGIATAAAEAKAEEIA
                     PVRPDVDLPPITPPGVKPVRTGRRSAGLIGGIDLGGTKIAAAVLDADHKVIAYRRRPT
                     PDRGGPADVVRAMAVTMQEAALDAGTEAHRLLAVGVGSPGSVDPDSGAVSGAGNLPDW
                     DGTFQLRLALQEELGTEVRVGNDVQVGTRGEYELGAGAGYSSLLGVFWGTGVGGGIII
                     NGEPWLGRGRAGEIGHTLVRRGGAKGLNGLNGTVEAYAGRAAMEEKARREVKKGRKTK
                     LFKLMKKHEKTHLTSAIWQHALDQDDELAQDLLNRAVAALSAGIASAVNLLDPEAVVI
                     GGGLGVRFADTLVPEIIAGMDQYLLNKDNPPAVKVAALGDEGGVIGASLLVKELVEAN
                     QAPTA"
     gene            complement(2485..>3183)
                     /locus_tag="PLJ64_04670"
     CDS             complement(2485..>3183)
                     /locus_tag="PLJ64_04670"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNC92816.1"
                     /translation="PWGSMAERAVITEGSGITLPPGIEPEQAIAFGTAGMAAWLSTDW
                     RGQLKDGETVLVLGASGSVGQVAVQAARMMGAGRVVGMARSEAGRERVLALGADAAIA
                     SDGSVEELKARIFEATEGGQDLVIDGLWGLPAAAALQTMKPHGRLVQVGNSAGTEAPI
                     AAGPVRGAAAAILGHRNFWPPREVRVATFERMCSLATSGDLELEVEVLPLDEVAEAWD
                     RQFRSPGHKLALRP"
     assembly_gap    3184..3283
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3315..6152)
                     /gene="uvrA"
                     /locus_tag="PLJ64_04675"
     CDS             complement(3315..6152)
                     /gene="uvrA"
                     /locus_tag="PLJ64_04675"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016099277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNC92817.1"
                     /translation="MSRSERILITGAREHNLKGIDLDLPRDALIVVTGLSGSGKSSLA
                     FDTIYAEGQRRYVESLSAYARQFLGLMEKPDVDSIEGLSPAISIDQKTTSRNPRSTVG
                     TVTEIYDYLRLLWARIGHPHCPNCGKPITGQTQEQITDQLMTMEEGKRFMVMAPVVRG
                     RKGEYGKLFDQFRADGYSRVEVDGEMRMLDQEIVLDKKYKHDISVVVDRLVMKPDLRR
                     RLSESVEAAASLAEGLVEIEVIDGERQTFSESFACLECGTSMPELEPRIFSFNAPHGA
                     CPACTGLGFKKVIDPELVVPDPTLSVAEGALQPWRSGYSGYWRRLNKSVAETYGIDIN
                     TPWQELTQKERDVFLYGTGTERHTIRTKSRAGRQRSYQVKFEGLVNNLERRFQESDSE
                     AVREKIEDYMAEQPCPSCHGARLRPESLAVTVGGLSIRDFTDMSATAAREWIGNLDLT
                     ATERAIARLIVREVEERLSFLVNVGIGYLSLARSARTLSGGEAQRIRLATQIGSSLVG
                     VMYVLDEPSIGLHQRDNEKLIATLERLRDLGNTVIVVEHDEGTMRAADYLVDLGPGAG
                     EHGGSVVAAGTPVEVSANPDSLTGAYLVGSEQIAVPESRRSWEKALTVKGARQHNLTG
                     VDVEFPLGVLTCVTGVSGSGKSTLVNETLFPAVANRLHKAKLRPGPHDRIDGMEEIDK
                     IINIDQSPIGRTPRSNPATYTGVFDQIRQLFAATQESRARGYKPGRFSFNVKGGRCEV
                     CKGDGQIKIEMHFLPDVYVPCEQCHGHRYNRETLEVRFKGKSIADVLEMSIEEAYPFF
                     ESIPKVARRLKTLNDVGLGYVRLGQPATTLSGGEAQRIKLAAELSKVATGNTLYILDE
                     PTTGLHFGDVRRLLEVLGRLVDAGNTVVVIEHDLDVIKSADWIVDLGPEGGDGGGHIV
                     ATGTPEEVARVQGSYTGDFLRGVLEPVALAA"
     gene            complement(6225..6698)
                     /locus_tag="PLJ64_04680"
     CDS             complement(6225..6698)
                     /locus_tag="PLJ64_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92818.1"
                     /translation="MEREHLVRTDFPRAHGSEGFDIDSVGAHLAAVAAHVAALEARIT
                     ALQVECDTLRTQLADARDHEDGPSGLPDPAGPDPLQKLGAEVEQAPVETMPADPEPDP
                     EAGAAPGEEEAAARMLASKLAMGGTSRDTIVMQVAAQHEIDSPGALVDDVLARLA"
     gene            complement(6739..8736)
                     /gene="uvrB"
                     /locus_tag="PLJ64_04685"
     CDS             complement(6739..8736)
                     /gene="uvrB"
                     /locus_tag="PLJ64_04685"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNC92819.1"
                     /translation="MAEFKLNPAFKPLADQPAAIEQLTEGVEAGEKIQTLLGATGTGK
                     TFTMAGVIAETQRPALVMAHNKTLAAQLCNEFREFFPDNVVEYFVSYYDYYQPEAYVP
                     AQDLYIEKDSSINDEIDRLRHAATASLLARRDVIIVASVSCIYGIGSPALYQQQMQLF
                     KVGEWIDRDKVLRKLVRLQYERNDTVLSRGSFRVKGEVLEIMPSYAESAFRIMLFGDE
                     IEAIHHFDPLTGEVLDEVKHVSIWPATHYVTEEDSVERTLEAIRTELDQRLEELESEG
                     KELEAHRLRQRTLYDLEMIKETGFTSGIENYSRLFEGRAAGTPPHTLIDYFPEDFITF
                     VDESHQTIPQIGGMYLGDRSRKQTLVDFGFRLPSAIDNRPLKFDEFMDRMNQLVLVSA
                     TPGEFERTESTRVVEQIVRPTGIVDPEIDVRPTANQIDDLMNEIRATTDRGERTLVTT
                     LTKKMAEDLTNYLIEHGVKVRYLHSDIDTLERIQIIRELRLGEYDVLVGVNLLREGLD
                     MPEVSLVAILDADKEGFLRGETSLIQTIGRAARNTAGRVLMYGDKETEAMRSAISETD
                     RRRAIQVAYNERHGVTPTTIVKGVAEEGNLLAIEDRAPARRRRRSEDEFEGPDELEKS
                     IVQMEEEMHAAAEDLRFEQAARLRDEIRELRRDLDAMKAGA"
     gene            8770..9147
                     /locus_tag="PLJ64_04690"
     CDS             8770..9147
                     /locus_tag="PLJ64_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92820.1"
                     /translation="MRTLRRALGLAFIGVGILHFARPKFFLSIMPSWIPLHREAVAVS
                     GAAEIVGGVAMLPDRTASLAAPWLVALLVAVFPANVNMAIDQQQIEQAKASGIPLWAL
                     WARLPLQPLLMYLVWKSTRPQDG"
     gene            complement(9152..10090)
                     /locus_tag="PLJ64_04695"
     CDS             complement(9152..10090)
                     /locus_tag="PLJ64_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92821.1"
                     /translation="MTPDEPTDEMPGGPEDPTEVNSPGDEPTEVNPPGNEPTEVNPRG
                     DQASQVIPPGSRPAAAPDPGEQAGSRGPASRGQRGIGVLIGSIVASLVLVGAYALAGG
                     PVAPDGPADPCDARPWTDPQNLEEAAQQFALSAADGAACELGVSREELTRSIADDQAR
                     QDFMDEHGFSESDLEDALRSGLNRAVDDAENAGAISGITPALLRAAIKVMPMDTMIGL
                     LENADSIFNGDFGSMGGLGDALNAITGGFDGLGNSGDPGNSNDPLGSLGDALEGALPE
                     GTTDDLNQIIPEEERQKLEDQAREKLKQGLEDFLGP"
     gene            complement(10087..11451)
                     /locus_tag="PLJ64_04700"
     CDS             complement(10087..11451)
                     /locus_tag="PLJ64_04700"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC92822.1"
                     /translation="MNLRTAALAGAVGLVLADSSIVGLALPEIFRQFDTSISGVSWVL
                     IIFNLVLALLAVPAAHMARRFGPGRSAAVGLAVFAGGSLVCGLATGLGPLLTGRAVQA
                     MGGAVAVVAALELMVSTFGDDRRAIATWVGAGAIGAAIGPGLGGLLTELVSWQSIFLV
                     QVPVAIVAGVPLKDIVIQETREWKIPDPVQPVEGNRPHLPANLALALVSASIAATLFL
                     IVLMLIEGWLLTPIEAALVVTVLPVAALVGSRIGNGIESERIRAASGAILLAGGLAAL
                     GLLPKATVWLVIPPQILAGLGLSLVLSALTEAALHGRSFAAVHGGWTISARHAGVVIG
                     LLILTPIFTHQLDVQKEAALDAGTAIVLDSPIDPSAKIDLGDKLANEVEAQGDRVPDL
                     SPAFEPMPTDPEDHARYEEILTGIESQLKDAATKAFSLSFLISALFGLLALIPIGMTR
                     RLDL"
     gene            complement(11451..12095)
                     /locus_tag="PLJ64_04705"
     CDS             complement(11451..12095)
                     /locus_tag="PLJ64_04705"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNC92823.1"
                     /translation="MAQNRTSTAGGRRRSNPAPRRGSTKPRTGAGRAFLILAGLIAVG
                     ILAILIISGLASKGIKEVALPLKHEDIIRQQSQEKGVDASLIAAVIYAESQFVDQTSH
                     ADARGLMQITPATAEEIEKLSGGTTFKLEDLRDPELNIRYGTFYLAHLLEMYDGDVVA
                     ALAAYNGGPGNANEWGGADMTIDDITFPETREYVKKVLEKQREYKHEYSLELGY"
     gene            complement(12114..12698)
                     /gene="coaE"
                     /locus_tag="PLJ64_04710"
     CDS             complement(12114..12698)
                     /gene="coaE"
                     /locus_tag="PLJ64_04710"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNC92824.1"
                     /translation="MTGGVAAGKSEALSAFGRLGAATISADQVVHDLLDREPLLGRLK
                     ERWGDEVAPEGRVDRGVVGAKVFNDPEELAWLESQIHPLVGEEFRGWIEGLDPATKFA
                     VAEIPLLFEGEMYRAFDHTVAIVAHEDTRQERARSRGHEGVEGREARQLSQAEKAERA
                     DTVIENDGTPAELESKLATLLEKFESELATGGQS"
     gene            complement(12707..14239)
                     /gene="rpsA"
                     /locus_tag="PLJ64_04715"
     CDS             complement(12707..14239)
                     /gene="rpsA"
                     /locus_tag="PLJ64_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HNC92825.1"
                     /translation="MTTTETTLQPAPEGVPVEGSNGLLINVDGRIIPNYAATIVSFEE
                     GDVVNGKVVRIDRDEVLLDIGYKSEGVIPAGELSIRKNVDTSEEVELGEEIDALVVTK
                     EDSEGRLILSKKRARFEKAWRKIEAAAENGEPVEGNVIEVVKGGLILDLGIRGFLPAS
                     LVDIRRVHNLDEFMSTTLECKVIELNRSRNNVVLSRRAVLEEERKEVRDQILDRLQPG
                     EVVEGKISNIVDFGAFVDLEGIDGLIHISELSWSHVNHPSEVVAIGDTVKIKVLDIDR
                     DRQRISLGLKQTQEDPWQRVVSTYRSGDVLEGTVTKVVSFGAFVEILDGVEGLVHISE
                     LADHHVENPSEVVELGASLNVKILEIDEERRRLSLSIKRVEGQNMLLKDLGAALAAAS
                     GEPAVEEETPELGLSEEVFADDAEAPADSAEEVAPEVEAVVDEAIAEAEVADAAEQAE
                     AADETPEEVVEDAVAEAEIEAAADEAAAEEAVVEEAGEITEEAAAEEAAEGEAPEGEE
                     QS"
BASE COUNT         2276 a         4728 c         4718 g         2502 t
ORIGIN      
        1 caggaccagc gcggctgaat gccgcgcgtg cgtccgtccc ggcgatcctc acccggcccc
       61 gcggcggacc acgaaggttc cgcgggtttg cctaccccgc tgaatcagtg gtcgaggaat
      121 tcgacttcct tgatgacctt ggccggggag ccggccgaga ttgtgttgtc agggaggtca
      181 gtggtgacca cggagttggc gccgatcacg cagtgatcgc cgatcgttac gccggaggtg
      241 acgacgcagt tgactccgag ccagacattg gagccgatcc tgaccggtcc cttgctggtg
      301 aagccctgcc aggtgatcgg aatggtcggg tcgttgaagc ggtggctggc gtcgccgacg
      361 aaacagccgt tggcgaacat cacgtggtca ccgatcgtga cctcgttttc ggccgccagc
      421 atcacgttgc ggttgagcca gacgtcatca ccaagcgtga cccgggcgtg gtcggacatg
      481 gtcagccagc aacccggttc aaagtggcag ccctggccga tctcgagcct tccgtcacgc
      541 agcgccccga gcacgtcgcc ctcgatcggg tgcctcacgt aggagccgcg gcgggccatc
      601 tctagatcga gccggaaccg ggagagcggc cggtgggaac gctcgtacca gcgccgcttc
      661 tgccaccaca tcttcagtcc acgctggtcg ctcatgccaa taccttgcca gaggtggata
      721 gtgtgcggcc atgacggcga tcaaggtttt ctctgatgct caggcgtaag gtcgatcaac
      781 ccgaagatca ggcggcggaa accggggccg cggaagctgc tgaggctgcc ggaactgcgc
      841 ccgagacgga acctgttccc gagtccgccg taaccgggtc ggagtctgca tcaaacgagc
      901 ccgagcccgc ccagcccgaa cccgggtccg cttcacccga ggagactgcg gccgccggaa
      961 ccgaacccgg gtccgcgccg gccgaggtcg aaccggccgg cgtgctcgag gaggatgtcc
     1021 gcgacgaaca gatcgaggtg acccccggac cggtggcctc ccattcgccc gagccccaga
     1081 ccccggccga gcagctgacc gcccggcagc aatccgtcca ccaggcgcca gagccggatt
     1141 caaaggactt catgaaggat cgccgcgagg cctccaaagc gcagcggaag gccgacaagg
     1201 agaagcgcaa ggccgaccgc aaggcggcca aggaagcccg caaggccagg cgacagagcg
     1261 tcggccagag cgaggccatg gccctcgatc cgctggcccc gaccacgccc ggcccggtcg
     1321 ctccgcctcg gccaccgaac ccgccggtcg gttcgcctgc cgccggcatc gccaccgcgg
     1381 ccgctgaggc caaagccgaa gaaattgccc cggtgcggcc ggatgtggac ctgccgccga
     1441 tcaccccgcc gggtgtgaag ccggttcgta ccggccggcg ctccgccggg ctgatcggag
     1501 gcattgacct cggcggcacc aagatcgcgg ccgccgtcct cgatgccgac cacaaggtga
     1561 tcgcgtaccg ccggcgtccg actccggatc gcggcggccc cgccgacgtg gtgcgggcga
     1621 tggcggtgac catgcaggaa gccgcgctcg acgccggaac cgaggcccat cggctgctcg
     1681 ccgtcggggt gggatcgccc gggtcggtcg accccgattc cggcgccgtg agtggcgccg
     1741 ggaacctgcc ggactgggac ggcaccttcc agctgcgact ggcactccag gaagaactgg
     1801 gaacggaagt ccgggtcggc aacgacgtcc aggtcggcac ccgcggcgag tacgagctcg
     1861 gcgccggggc gggctactca agccttctcg gggtcttctg gggcaccgga gtcgggggcg
     1921 ggatcatcat caacggcgag ccgtggctcg gccgcggccg ggccggagag atcggtcaca
     1981 cgctggttcg ccgcggtggc gccaagggcc taaacggcct caacggcacg gtcgaggcct
     2041 atgccggtcg cgccgcgatg gaggagaagg cccgccggga agtcaagaag ggccgcaaga
     2101 ccaagctctt caagctgatg aagaagcatg agaagaccca cctcacttcg gcgatctggc
     2161 agcacgcact cgatcaggac gacgagttgg cccaggacct cctcaaccgt gcggtcgcag
     2221 ccctttccgc ggggatcgcc tcggcggtca acctgctcga tcccgaagcg gtggtcatcg
     2281 gcggcgggct cggcgtccgg tttgccgaca ccctggtccc ggagatcatc gccggcatgg
     2341 atcagtacct gctgaacaag gacaatccgc cggccgtaaa ggttgccgcg ctcggagacg
     2401 agggtggcgt gatcggcgct tccctgctgg tcaaggaact cgtcgaggca aaccaggcac
     2461 caaccgcctg acggctggca agcctcaggg tctgagcgcg agtttgtggc cggggcttcg
     2521 gaactgacgg tcccaggcct cggccacctc gtctagcggc aaaacctcga cctcgagttc
     2581 gaggtcgccg ctggtcgcca gcgaacacat ccgctcgaag gtcgcgaccc gcacctcccg
     2641 cggcggccag aaattgcggt ggccgagaat cgccgcggct gctccccgca ccggcccggc
     2701 ggctatcggc gcttcggttc cggccgaatt gccgacctgg accagcctgc catgtggctt
     2761 catggtctgg agagccgcgg cggccggcag gccccacaag ccgtcgatca ccaggtcctg
     2821 cccgccttcg gtcgcctcga agatccgggc cttcagctcc tcgacgcttc cgtcggaggc
     2881 gatcgccgca tccgcgccca gcgcgagaac cctctcgcgg ccggcttcgc tccgggccat
     2941 gccgaccacg cggccggccc ccatcatccg ggccgcctga accgctacct ggccgaccga
     3001 gccgctggct ccgaggacca gcacggtctc gccatccttg agctgcccgc gccagtcggt
     3061 cgaaagccag gccgccattc cggcggtccc gaaggcaatc gcctgttcgg gttcgatccc
     3121 cgggggaagc gtgatgccgc tgccttcggt gatcactgcc cgttcagcca tcgaacccca
     3181 gggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngcttcgc cgtaagcggt
     3301 tacctgggca gctttcatgc cgccaaagct accggctcga ggaccccgcg caggaagtct
     3361 ccggtgtagg agccctggac cctggcgact tcctcgggcg ttccggtggc gacgatgtga
     3421 ccgccaccgt cgccgccctc ggggccgaga tcgacgatcc agtcggcgct cttgatcacg
     3481 tcgaggtcat gctcgatgac cacgacggtg ttgccggcat caaccagccg gcccagcacc
     3541 tcgagcagcc gtcgcacgtc tccgaagtga aggccggtgg tcggctcgtc gaggatgtaa
     3601 agcgtgttgc cggtcgcgac cttggagagc tcggcggcca gcttgatccg ctgcgcctcg
     3661 ccaccggaga gagtcgtggc cggctggccg agccgcacgt agccgagccc cacgtcattc
     3721 agggtcttca ggcgacgggc caccttcggg atcgattcga agaagggata cgcctcctcg
     3781 attgacatct ccaggacatc ggcgatcgac ttgcccttga accggacctc cagggtttcc
     3841 cggttgtaac ggtggccgtg gcattgctcg cagggcacgt acacatcggg caggaagtgc
     3901 atctcgatct tgatctgacc gtcgcccttg cagacctcgc agcggccgcc cttgacgttg
     3961 aagctgaacc ggcccggctt gtagccgcgg gcccgggatt cctgggtggc cgcgaaaagc
     4021 tgccggatct ggtcgaagac cccggtgtag gtggccgggt tcgagcgcgg cgtgcggccg
     4081 atcggcgact ggtcgatgtt gatgatcttg tcgatctcct ccatcccgtc gatgcggtca
     4141 tgcggcccgg gcctcagctt tgccttgtgg aggcggttgg ccacggccgg gaacagcgtc
     4201 tcgttgacca gggtcgattt gccggaacca gaaacgccgg tcacgcaggt caggaccccg
     4261 agcgggaact cgacgtccac cccggtcagg ttgtgctggc gggctccctt cacggtcagg
     4321 gccttttccc agctgcgccg gctctccggc accgcgatct gctcggagcc gaccaggtag
     4381 gcgccggtca gtgaatcggg gttggcggaa acctcgaccg gcgtgccggc cgcgaccacg
     4441 ctgccaccgt gttcgccggc cccgggcccg aggtcgacca ggtagtcggc cgcccgcatc
     4501 gtgccttcgt cgtgctcgac cacgatcacc gtgttgccga ggtcacggag cctttccagg
     4561 gtcgcgatca gcttttcgtt gtcacgttga tgaaggccga tcgagggttc gtccagcacg
     4621 tacatgacgc cgaccaggct ggagccgatc tgggtcgcca gccggatccg ctgggcctcc
     4681 ccgccggaaa gggtccgggc ggaccgggcc agagagaggt agccgatccc cacgttgacc
     4741 aggaaggaaa gccgctcttc gacctcccgc acgatcagcc gggcgatcgc ccgttcggtg
     4801 gcggtgagat ccaggttccc gatccattcg cgggccgcgg tggccgacat gtccgtgaag
     4861 tcccggatcg agagaccccc gaccgtgacc gcgaggcttt ccggcctgag ccgggcaccg
     4921 tgacagctcg ggcagggctg ctctgccatg tagtcctcga tcttctcccg aaccgcttcg
     4981 gagtccgatt cctggaagcg gcgttcgagg ttgttgacca ggccttcgaa cttgacctgg
     5041 tagctgcgct ggcgtccggc ccggctcttg gtccggatcg tgtggcgttc tgtaccggtg
     5101 ccgtagagga acacgtcccg ttccttctgg gtcagttcct gccagggcgt gttgatgtca
     5161 atgccgtagg tctcggcgac cgacttgttg aggcggcgcc agtatccgga gtagccggaa
     5221 cgccagggct gcagcgcgcc ttcggccacc gacagggtcg ggtcgggcac gaccagttcc
     5281 gggtcgatca ccttcttgaa gccgagcccg gtgcaggccg gacaggcgcc gtggggcgcg
     5341 ttgaaggaga agatgcgcgg ctccagttcc ggcatcgaag tgccgcattc gaggcaggca
     5401 aaactctccg agaaggtctg gcgctcgccg tcgatcacct cgatctctac caggccttcg
     5461 gccagtgacg ccgctgcttc gaccgactcg gagaggcggc ggcgaaggtc cggcttcatg
     5521 accagccggt cgacgaccac cgagatgtcg tgtttgtact tcttgtcgag gacgatctcc
     5581 tggtccagca tccgcatctc gccgtcgacc tcgacccgcg agtatccgtc ggcgcggaat
     5641 tgatcgaaga gcttgccgta ctcgcccttc ctgccgcgaa ccaccggcgc catgaccatg
     5701 aaccgcttgc cctcctccat ggtcatcagc tggtcggtga tctgctcctg ggtctgcccg
     5761 gtgatcggct tgccgcagtt cgggcaatgc gggtgaccga tccgggccca gaggaggcgc
     5821 aggtagtcgt agatctcggt caccgtgccg accgtcgaac ggggattccg cgaggtcgtc
     5881 ttctggtcaa tcgagatcgc cggagaaagc ccttcgatcg aatcgacatc gggcttttcc
     5941 atcagtccga ggaactgcct ggcgtaggcg gaaagcgact cgacatagcg gcgctggccc
     6001 tccgcataaa tcgtgtcgaa ggcaaggctg gacttgccgg aacccgacag tccggtcacc
     6061 acgatcaggg catcgcgagg caggtcgaga tcgatgcctt tgaggttgtg ctcacgggcg
     6121 ccggtaatca ggatgcgttc agaacggctc acgcccccca atattaggga accgaacaca
     6181 cgttcgtttg cacccgaacc ccttccatgc cgcgcagggc gggttcaggc gagtcgggcg
     6241 agcacgtcgt cgacgagagc gccgggactg tcgatctcgt gctgcgcggc gacctgcatc
     6301 acgatcgtgt cgcggctggt accgcccatc gcgagtttcg aggcgagcat ccgggccgcc
     6361 gcctcttcct cgccgggtgc ggctcccgcc tcgggatccg gttcggggtc ggccggcatc
     6421 gtctcgacgg gcgcctgctc gacctcggca cctagcttct gcagcgggtc aggcccggcc
     6481 ggatcgggaa ggccgctcgg accgtcctcg tgatcgcgcg catcggcaag ctgggttcgc
     6541 agggtgtcgc attccacctg cagggcggtg atccgggctt ccagcgcggc aacgtgcgcc
     6601 gccaccgcgg ccaggtgggc gccgaccgag tcgatgtcga aaccttcgga cccgtgggcc
     6661 cgcggaaaat ccgttcgaac caggtgctct cgctccattc agaaagccta gcgctccccg
     6721 gaccgcggcc gttccggctc aggccccggc cttcatcgcg tcgagatccc ggcgcagttc
     6781 ccggatctcg tcacgcagac gggcggcctg ctcgaagcgg agatcctcgg cggcggcatg
     6841 catttcctct tccatctgga cgatcgactt ctccagctcg tccgggccct cgaactcatc
     6901 ctcgctgcgc cgccggcggc gggccggggc cctgtcctcg atcgccagca ggttgccttc
     6961 ctcggccacg cccttgacga tcgtggtcgg ggtcacgcca tggcgttcgt tgtaagcgac
     7021 ctggatcgcc cggcggcggt cggtctcgga gatcgcggag cgcatcgcct cggtctcctt
     7081 gtcgccgtac atcagcaccc ggccggcggt gttccgggcg gcccggccga tcgtctggat
     7141 caggctcgtc tcgccgcgca ggaacccttc cttgtcggcg tcgagaatcg cgaccagcga
     7201 gacctccggc atgtcgaggc cctcacgcag caggttgacc ccgaccagca cgtcgtactc
     7261 ccccagcctg agttcgcgga tgatctggat tcgctcgaga gtgtcgatgt ccgagtggag
     7321 atagcgaacc ttgactccgt gctcgatcag gtagttggtc aggtcctcgg ccatcttctt
     7381 ggtcagggtg gtgaccaggg tgcgttcgcc gcggtcggtc gtcgcgcgga tctcgttcat
     7441 caggtcgtcg atctggttgg cggtcggccg cacgtcgatc tcgggatcga cgatgccggt
     7501 cggccggacg atctgctcga ccacccgggt ggattcggtc cgctcgaact cgcccggggt
     7561 cgccgaaacc agcaccagct ggttcatcct gtccatgaac tcgtcgaact tgagcgggcg
     7621 gttgtcgatc gccgagggca gccggaagcc gaagtcgacc agggtctgtt tgcgggagcg
     7681 gtcaccgagg tacatgccgc cgatctgggg gatggtctgg tgggactcgt cgacgaaggt
     7741 gatgaagtcc tcggggaagt agtcgatcag ggtatgcggc ggggtgccgg ccgcgcgacc
     7801 ctcgaacagc cgcgagtagt tctcgatgcc ggaggtgaag ccggtttcct tgatcatctc
     7861 gaggtcgtag agggtgcgct ggcgaaggcg gtgggcctcg agttccttgc cctcggattc
     7921 gagctcctcg agacgctggt caagctcggt ccggattgcc tccagggtgc gttcgaccga
     7981 gtcctcctcg gtcacgtagt gggtggccgg ccagatcgag acatgcttga cctcgtcgag
     8041 gacctcgccg gtgaggggat cgaaatggtg gatcgcctcg atctcgtcgc cgaagagcat
     8101 gatccggaag gccgactcgg cgtaggaggg catgatctcc agcacctcgc ccttgacccg
     8161 gaaggagccg cggctcagca ccgtgtcgtt tcgttcgtac tgcagccgga ccagcttgcg
     8221 caggaccttg tcgcggtcga tccactcccc caccttgaac agctgcatct gctgctggta
     8281 gagagccggc gagccgatgc cgtagatgca tgagaccgaa gcgacgatga tcacgtcccg
     8341 ccgggccagc agcgaggcgg tcgcggcgtg acgcaggcgg tcgatctcgt cgtttatcga
     8401 ggagtccttc tcgatgtaga gatcctgggc cgggacatag gcctccggct ggtagtagtc
     8461 gtagtaggag acgaaatact cgaccacgtt gtcgggaaag aactcgcgga actcgttgca
     8521 gagctgggcg gcgagggtct tgttgtgagc cattaccagg gccgggcgct gggtctcggc
     8581 gatcaccccg gccatggtga aggtcttgcc ggtcccggtc gcgccgagca gagtctggat
     8641 cttctcgcca gcctcaaccc cttcggtcaa ttgctcgatc gccgcaggct ggtcggccag
     8701 cggcttgaag gctggattga gcttgaactc ggccacccgc ccaccataac gacccgttag
     8761 gttcgaccca tgcgaactct tcgccgcgcc ctgggcctcg ctttcatcgg agtcggaatc
     8821 cttcactttg cccggccgaa gttcttcctt tcgataatgc cttcgtggat cccgctccac
     8881 agggaggcgg tcgcggtcag cggggcggcc gagatcgtcg ggggtgtggc gatgcttccc
     8941 gaccggaccg ccagcctggc ggcaccctgg ctcgtcgccc tgctggtggc ggtctttccg
     9001 gccaatgtca acatggcgat cgaccagcaa cagatcgaac aggcgaaagc aagcggcatc
     9061 cccctctggg cgctctgggc ccgactgccg ctccagccgc tgctcatgta cctggtctgg
     9121 aagtcaaccc gcccgcagga cggatgacgg ctcacggccc gaggaagtcc tcgaggccct
     9181 gcttgagctt ctcccgggcc tgatcctcga gcttctggcg ctcttcttcc gggatgatct
     9241 ggttgaggtc gtcagtggtg ccctccggca gggcgccttc gagggcgtcc ccgaggctgc
     9301 cgagcggatc gtttgaattg ccggggtcgc cggagttgcc gaggccgtcg aaaccgccgg
     9361 tgatcgcgtt cagcgcatca ccgagtccgc ccatcgaacc gaaatccccg ttgaagatgc
     9421 tgtccgcgtt ttcgagcagt ccgatcatcg tgtccatcgg catcaccttg atcgcggccc
     9481 gcaggagcgc cggggtgatg ccgctgatcg cgccggcgtt ctcggcgtca tcgaccgccc
     9541 ggttgaggcc ggaacggagc gcgtcttcaa ggtcggattc gctgaacccg tgctcgtcca
     9601 tgaagtcctg ccgggcctgg tcgtcggcga tcgagcgggt gagttcttcc cggctgactc
     9661 cgagttcaca ggctgcgccg tcggcggccg acagtgcgaa ttgctgggcg gcttcctcga
     9721 gattctgggg atcggtccag gggcgggcat cgcatgggtc ggccgggccg tccggggcta
     9781 ccggcccacc ggccagcgcg taggccccga ccagaaccag cgaagcgacg atcgatccga
     9841 tcagcacccc gattccccgc tggccccgac tggccggacc gcgcgacccg gcctgttcac
     9901 ccggatctgg tgccgccgcc ggcctgctgc cgggcggaat cacctgtgac gcttggtcgc
     9961 cgcgcgggtt cacctcggtc ggctcgttcc ccggcgggtt cacctcggtc ggctcgtcgc
    10021 ccggcgaatt cacctcggtc ggatcctccg ggccgcccgg catttcgtct gtcggttcgt
    10081 ccggggtcaa aggtccagtc tccgggtcat gccgatcggg atcagggcca gcagcccgaa
    10141 cagcgccgag atcagaaacg aaagcgagaa agccttggtc gcagcatcct tcagctggga
    10201 ttcgatcccg gtgagaatct cctcgtaccg ggcgtggtcc tcgggatcgg tcggcatcgg
    10261 ttcgaaggca ggagagagat ccggcactcg gtcgccctgg gcttcaacct cgttggcgag
    10321 cttgtccccg agatcgatct tggccgaggg atcgatcggg gaatcgagca cgatcgcggt
    10381 accggcgtcg agggccgcct ctttctgcac gtcaagctga tgggtgaaga tcggagtgag
    10441 gatcaacagg ccgatcacta cgccggcgtg ccgggcggaa atagtccagc cgccgtggac
    10501 cgcggcaaaa gagcgtccgt gaagggcggc ctcggtcaac gccgacagga cgagggagag
    10561 cccgagacca gccaggatct ggggcgggat taccagccag acggtcgctt tcggaagcag
    10621 cccgagcgcg gccaggccac cggccagcag gatcgccccg gaagcggcgc ggatccgttc
    10681 cgattcgatc ccgttgccga ttctcgaccc gaccagggca gctaccggca gcaccgtcac
    10741 caccagagcc gcttcgatcg gagtcagcag ccagccctcg atcagcatca gcacgatcag
    10801 gaagagcgtc gccgcgatcg aggcggagac cagggccagg gccaggtttg ccggcagatg
    10861 cgggcggttg ccctcgaccg gctggaccgg gtccgggatc ttccactcac gggtttcctg
    10921 gatcacgatg tccttgagcg gcacgccggc gacgatcgcc accggcacct ggaccaggaa
    10981 gatcgactgc caggaaacga gctcggtcag aaggccaccg agcccgggtc cgatcgcggc
    11041 accgatcgca ccggcgccaa cccaggtggc aatcgcccgg cgatcgtcac cgaaagtgga
    11101 aaccatcaac tcgagcgcgg cgaccaccgc gacggcaccg cccatggcct ggaccgcgcg
    11161 accggtcagg agcggcccga ggccggtcgc cagcccgcag accaggctgc cgccggcgaa
    11221 gacggccaga ccgaccgcgg ctgagcgacc gggaccgaac cggcgggcca tgtgggcggc
    11281 cggcacggcg agcagggcca ggacgaggtt gaagatgatc agcacccagg agacaccgga
    11341 gatgctggtg tcgaactggc ggaagatctc cggcagggcg agtccgacga tcgacgagtc
    11401 ggcgagaacc aggccgaccg cacccgccag cgcggccgtg cgcaggttca tcagtacccg
    11461 agctccaggg aatactcgtg tttgtactcc cgctgcttct cgaggacttt cttcacgtat
    11521 tccctggttt cggggaaggt gatgtcatcg atcgtcatgt cggctccgcc ccattcgttg
    11581 gcgttgcccg gcccgccgtt gtaggcagca agggccgcga ccacgtcacc gtcgtacatc
    11641 tccagcaggt gagcgaggta gaaggtgccg tagcggatgt tcagctccgg atcgcgcaga
    11701 tcctcgagtt tgaaggtggt gccaccgctc agcttttcga tctcctccgc ggtggccggc
    11761 gtgatctgca tcaggcccct cgcgtcggcg tgggaagtct ggtcgacgaa ctgggattcg
    11821 gcgtagatca ccgcggcgat cagcgaggca tcaacacctt tttcctgcga ctgctgccgg
    11881 attatgtcct cgtgcttcag gggcaacgcc acttccttga ttcccttgct ggccagcccg
    11941 gagatgatca ggatcgcgag gatgccgacc gcgatcaggc cggccaggat caggaaggcc
    12001 cggcctgcgc cggtgcgcgg tttcgtgctg ccgcgccggg gggccgggtt tgagcggcgt
    12061 cgaccgcccg ctgttgaagt tcggttctgg gccatgcgag gtggccttat agcctagctt
    12121 tgcccgccgg tcgccaactc tgactcgaac ttttcgagca gggtcgccag cttcgattcg
    12181 agttcggccg gggttccgtc attttcgatg accgtgtctg cccgctcggc cttttcagcc
    12241 tggctcagct gccgggcctc ccggccctcg accccttcat gcccgcgcga ccgggcccgt
    12301 tcctggcggg tgtcttcatg cgccacgatc gcgacggtgt gatcgaaggc gcggtacatc
    12361 tcaccctcga agaggagcgg gatttcggcc accgcgaact tcgtcgctgg atcgagaccc
    12421 tcgatccagc cgcggaattc ctcgccgacc agcggatgga tctgcgattc gagccaggcc
    12481 agctcttccg ggtcgttgaa gacctttgcc ccgaccaccc cgcgatccac gcggccttcc
    12541 ggggcaacct cgtctcccca gcgctctttg aggcgaccca gcagcggctc ccggtcaagc
    12601 aggtcgtgaa cgacctgatc ggcagagatt gtggccgcgc cgagccgccc aaaggcgctc
    12661 agtgcctcgg acttaccggc agctacgccc ccggtcagtc cgatcgtcag gactgttctt
    12721 ccccttcggg agcttcacct tcggctgcct cttccgcggc ggcttcttcg gtgatctcac
    12781 cggcctcctc gaccacggcc tcctcggcag cggcttcgtc agcggcagct tcgatctcgg
    12841 cctcggcgac ggcgtcctcg acgacctctt ccggggtttc gtccgcggcc tcggcctgct
    12901 cggcggcgtc ggccacttcg gcctcggcga tcgcctcgtc gactacggcc tcgacctcgg
    12961 gagcgacctc ttcggcgcta tcggccgggg cttccgcgtc atcggcaaag acctcttccg
    13021 agagacccag ttccggggtt tcttcctcga cggccggctc gccggaggcg gcggccaggg
    13081 cggcgcccag gtccttgagc agcatgttct ggccctcgac ccgcttgatc gaaagcgaga
    13141 ggcggcgacg ctcttcgtcg atctcgagga tcttgacgtt gaggctggcg ccgagctcga
    13201 cgacttccga cgggttctcg acgtgatggt cggccagttc cgaaatgtgg accagacctt
    13261 cgacgccgtc gaggatctcg acgaaagcac cgaaggagac gaccttggtg accgtgccct
    13321 cgagcacgtc gcccgaacgg taggtcgaga cgacccgctg ccacgggtcc tcctgggtct
    13381 gcttgagacc cagcgagatg cgctggcggt cgcggtcgat gtcgagcacc ttgatcttga
    13441 ccgtgtcgcc gatcgccacc acctcggagg ggtgattgac gtggctccag gaaagctcgg
    13501 agatgtgaat caggccgtcg atgccttcga ggtccacgaa agcgccgaag tcgacgatgt
    13561 tcgagatctt gccttcgacg acttcgcccg gctgcaggcg atcgaggatc tggtcgcgga
    13621 cttccttgcg ctcctcttcg aggactgcgc ggcgggaaag cacgacgttg ttgcggctgc
    13681 ggttgagctc gatgaccttg cactcgagcg tggtcgacat gaactcgtcg aggttgtgga
    13741 cccggcggat gtcaaccagc gaggcaggca ggaagccgcg gatgccgagg tcgaggatca
    13801 ggccgccctt gacgacctcg atcacgttgc cttcgaccgg ctcgccgttt tctgcggcag
    13861 cttcgatctt gcgccaggcc ttctcgaagc gggcacgctt cttggaaagg atcaaccggc
    13921 cttcggaatc ttccttggtg acgaccaggg cgtcgatctc ttcgccgagt tcgacctctt
    13981 ccgaggtgtc gacgttcttg cgaatcgaga gctcgccggc cgggattaca ccttccgact
    14041 tgtagccgat gtcgagcagg acttcgtcgc gatcgatgcg gacgactttg ccgtttacga
    14101 cgtcgccttc ctcgaacgaa acgatcgtcg ctgcgtagtt aggaatgatc cggccgtcaa
    14161 cgttgatcag caggccgttt gagccttcga ccggaacccc ctctggggcg ggttggagtg
    14221 tggtttcagt agtagtcaca gtccggggat gatggcagat atcaggagtc gggcggggtc
    14281 cgacccgccc tctatgttca acccgcccct cccattcgaa caaa
//
LOCUS       DAOQKN010000046        14280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_1983_length_14280_cov_5.516063, whole genome shotgun sequence.
ACCESSION   DAOQKN010000046 DAOQKN010000000
VERSION     DAOQKN010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 14280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14280
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1983_length_14280_cov_5.516063"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            229..1515
                     /locus_tag="PLJ64_04720"
     CDS             229..1515
                     /locus_tag="PLJ64_04720"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HNC92826.1"
                     /translation="MSALVALLAALPSPAVAAPPPLPDLVVESGTAPASATAGRQVSI
                     KVKVRNRGRKPAPVSRALVLLSRDAKRDVTDTKLGLVKIRKLARNKAVVSGGKLTIPS
                     GQPAGSRRVILCADGDRMIRESKESNNCRTLANAIQVMPGGTGPTGPTGPTGPTSPTG
                     PTGPTSPTGPTGPTGPTGPTTGLALSPSPLILGSLSYSFTNEESFDAIRYLTLTNLGP
                     GASQPISIWSLGMHGGQYADLMAIGESHGGLNGCFEGQSLAVGAQCSIHVSWAPRNRS
                     TTDMTGVVEIRLWNTTTVLASVPVSAAYQSRAYITGGLSPASLTANQGFKQRGDATLT
                     NVGDSPATPSVSVSGPDAQFFAVDSNRENTCEGFSIAVDATCKLPIVFCAGVVRDYEA
                     SLVVTVNNVVTESSPLRATVRQLNDHPQCTHLQTND"
     gene            1540..2121
                     /locus_tag="PLJ64_04725"
     CDS             1540..2121
                     /locus_tag="PLJ64_04725"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF014033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNC92827.1"
                     /translation="MVKIYTKKGDDGTTGLWYGGRVEKSGLRTDAYGTLDEACSALGV
                     ARAMCDDSQEELAADILALQNELFVAGAELATAPEAAGRLEDGISRITAGMVDALDPE
                     IDKYMAHVNLPPKFVIPGGNRLSAQLDVARTIVRRAERAVVRIQLADELASTEILRFL
                     NRASDLVFSMARYADVDFPDLFEGRRKSGEDEE"
     gene            2126..2926
                     /locus_tag="PLJ64_04730"
     CDS             2126..2926
                     /locus_tag="PLJ64_04730"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNC92828.1"
                     /translation="MDLGLRDRVCVVTGASGGIGLEVTRQLCDEGAKVLMVSRDPEQL
                     AAMREVVRAEGGEVEVVVKDITTDCAGQHIIAAARQAFGKVDCLVNNAGVAEWKPLEE
                     ASEEDFRFQFEMSVIAPLNLMRAAIPEMAERGWGRVVNVSSTAGKRPSSMMPDYSVGK
                     AAMLSLSRQFAERHAAEGVMVNAICPGPTASDLWMAEGGLLDQSKEAAGHESREEALK
                     AAGGKRPIGRLATPEEIASAIVFLCSERASYVSGAAWGVDGGTVPIII"
     gene            complement(3012..3548)
                     /locus_tag="PLJ64_04735"
     CDS             complement(3012..3548)
                     /locus_tag="PLJ64_04735"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HNC92829.1"
                     /translation="MASQTDYASLIRAIPDFPEPGILFRDITPLLLDAAACRSAVSDL
                     AEWAAPHKPDLLLAAESRGFILGGALAQALDIGFIPARKPGRLPSETIAVEYQLEYGS
                     DSLEVHSDSIFNGARVLVHDDLIATGGTARAKIDAVERLGGEVVGCAFLIELEGLGGR
                     HHIAPHKMHTLVTLPAQN"
     gene            complement(3558..3980)
                     /locus_tag="PLJ64_04740"
     CDS             complement(3558..3980)
                     /locus_tag="PLJ64_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92830.1"
                     /translation="MPRTGLLLALAVVASLALPGVAGAAKNGTYVGRDEGKVKVVLKI
                     RNNRVTYFEGNPAGSCYGIGSTLVAFRYPSAAQRPSASSRIKADGVFSAVFKGSRFVS
                     FKDDQRTLTGKVRGNKVSGRMIVSGLCQVDQRFTATRK"
     gene            4023..4592
                     /locus_tag="PLJ64_04745"
     CDS             4023..4592
                     /locus_tag="PLJ64_04745"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HNC92831.1"
                     /translation="MDFTTIDLPGLLLDTESAVAMKAHGSREAAVLIPIRNWPEAPRL
                     ILTERRADLRKHAGEISFPGGTSEPGDRDLEETALRESHEEIDLHPDQVEVIGAVPPV
                     GTFATSFRIQPIVGLVAEEAKVVANPDEVATILTFGFDELLDSYGMRRLVRRGVPIRT
                     PTFEMDRHLIWGATARILTDFFHRIGAMK"
     gene            4703..5497
                     /locus_tag="PLJ64_04750"
     CDS             4703..5497
                     /locus_tag="PLJ64_04750"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNC92832.1"
                     /translation="MAEALKFSIDGEIREAGEAFLPLPDDGLLRGDGVFEVARVYGGK
                     PFALDLHFDRMERSAATIELPLDRPGLERDAKNLIASVDDPDFLIRLVQTRAGRRILS
                     IEELPVYTPTISLATVTYSPTVILNGVKSLSYAANMQVTRLARAAGADEALFVRPDGV
                     VLELPTSTIFWATDDGRLRTPALEAGVLDSITRRKIVERLEVEQGEFPLEDLLAASEA
                     FVASTTREAHPVSRVDDREMPLVDGEQTRAAAAAFADALAEDLEAQ"
     gene            5494..6219
                     /locus_tag="PLJ64_04755"
     CDS             5494..6219
                     /locus_tag="PLJ64_04755"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC92833.1"
                     /translation="MTATPAISIRDLRVSRGGNLVLPDISVEIEKGSVTGLLGPSGCG
                     KTTLMRSIVGVQIVAGGTVEVLGRPAGSSDLRNRVGYVTQAPSVYGDLSVGENLDYFA
                     RVVGVSPERVAETIETVGLSGFTGRLVSSLSGGQRARVSLASALLNKPDLLVLDEPTV
                     GLDPLLRAELWKTFHELAESGTTLLVSSHVMDEAGACDHLILIRDGRLLRHTTPEALR
                     RETGENDLGRAFLKVIESDEEAA"
     gene            6216..6953
                     /locus_tag="PLJ64_04760"
     CDS             6216..6953
                     /locus_tag="PLJ64_04760"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC92834.1"
                     /translation="MSPRITLATGLRVLQQIRHDPRTIALLLLVPALLLTLMKYVFES
                     EPRVFQQIGAPLCGLFPFIVMFLVTSITMLRERTTGTLERLMTLPMSKLDLLLGYALA
                     FGLLAAAQALVICLVGFGLLGLNAPHGVLIIVGLAVLNALLGMSLGLLVSAFANTEFQ
                     AVQFMPAFIFPQLLLCGLFVARDQMAPLLEAVSWVMPFTWAYDALARAVSPQPLSGRV
                     AVDAAVVLAVTIGSLIFGAMTLRRRTA"
     gene            complement(6950..7894)
                     /gene="trxB"
                     /locus_tag="PLJ64_04765"
     CDS             complement(6950..7894)
                     /gene="trxB"
                     /locus_tag="PLJ64_04765"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNC92835.1"
                     /translation="MSDTEVENIIIIGGGPAGYTAALYAARAELNPLVFEGFQWGGQL
                     QNTTDVENFPGFPEGIMGPEMMTRFRDQAERFGTRFVTDDVDKVELTDGGIHKVYLGD
                     EVHHAKTVILAMGAEPKRLGIPGEMEMAGRGVSTCGVCDGAFFKDRSTMVIGGGDSAM
                     EDAVFISKFASSLDLVHRRDEFRASKIMQERAESLPNISLKTPWAPEEFVAGEDGALE
                     KVRLKHSESGEVEEIPVDGVFVAIGHIPRSELVVGQVDTDDEGYVKTEAASTKTNLAG
                     VFAIGDLVDHTYRQAVTAAGSGCKGALDAEWYLRDATF"
     gene            complement(7958..9082)
                     /locus_tag="PLJ64_04770"
     CDS             complement(7958..9082)
                     /locus_tag="PLJ64_04770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNC92836.1"
                     /translation="MTETPDRSEEEVEEGDGLPVFTTKGAVQTAVLIVLLIVAIYFLL
                     PKLAGFGDAIGMLGEAKPVYVGIAIGFCVLSFITYIALFRAVVGGESFPITWREAYEI
                     NMAGFAATLLFSAGGAGGVVLTYWALRQGGMERRDVGTRMVAFIVLHYLFYPLAVLVF
                     GILLGTGVLHGSTEVSLTFVPAAIALIVMLLGGALALVPADIERRIEGYARRHPGAKF
                     LARLSTVPAMVADGTRGALHLVSHPSRAGLAVVGAVGFWAANIGILWASFKAFGIAIP
                     LGVVVMGFFIGMVANLIPFVPAGVGAVDAGMIGTFVLFGFPEEQVFAAILIYRLVAFW
                     LPIPPGVAAFFQLRNRVKRWKKEGLPIDRPPRPSMIEEIL"
     gene            complement(9079..9921)
                     /locus_tag="PLJ64_04775"
     CDS             complement(9079..9921)
                     /locus_tag="PLJ64_04775"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNC92837.1"
                     /translation="MTVASTVSAPLAIAVLWPRSRRRDLGLFAAQMWAFAVCHELPYD
                     DPKRLRERLYVEYPIRIDRFIGRGQLPNARLQKWLHQGRYGNDLTGFAAWTHWFWFIQ
                     PYAAIFWIAVRNKEKFPESARQLAAVFDMGCAMYFLVPTAPPWWAAENGYIDPEIADE
                     HVKRVMLEYGEKLWGPAWTRIFGTLGSNPWAAMPSLHFATAVMAAILLSDAGGKVEGA
                     LGWTYALSLGFALIYLGEHYVTDLLVGAGVVLLARKGEPIVRPVVELINGRLQRLEAI
                     AAGG"
     gene            10145..10993
                     /locus_tag="PLJ64_04780"
     CDS             10145..10993
                     /locus_tag="PLJ64_04780"
                     /inference="COORDINATES: protein motif:HMM:NF012344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92838.1"
                     /translation="MSLFISLAGVLIAFALNASPAMALSLNPDAASPGIEDANTLHWI
                     LFVVILAVIVLVNLAILRAARPRTRAVSPKPGRKVSQVKLAIGLGVFALALFVVATVF
                     SDKSREIPAVNAEPVAGQVKGKPLEITATGSQWLWRFNYPNGAFSYRRLVVPAGVTVQ
                     LNLVSTDVIHGWNVPELTGKAQAVPGKTNEVYFRADDPGTYTSRSSVFSGQGYDTMEI
                     EVQVVPTDEYEAATIALKKDIQDAQDKVEAEFKASKAKAVVATAEGKKMKAEINKDDI
                     ESADSQ"
     gene            10990..12693
                     /locus_tag="PLJ64_04785"
     CDS             10990..12693
                     /locus_tag="PLJ64_04785"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNC92839.1"
                     /translation="MSRTTSYPQTMPPTRPEISSDGPGSRPSRWAELASSADHKDIGR
                     LLIATSFGSIFLAALELLLMRVQLAVPENVFLKGVTFDRLLSVYGETAIFLVALPLII
                     GLLYYVVPLQIGSRTTAIPRVGQIGLWLFIFGGLLLYGSFLFTPPEAGINPLPPFSDA
                     TFTPNNGVDVWIGSTGLITLGLLMIAIDLLATLHNLRAPGMAWRRAPLFTWAGAIVTW
                     LFVVIAPIFLAAISMLLIDRLYDGGFFAGDAGGSPILWQHLSYIFFTGVYMAVSITFL
                     AAIGEIIQTFTGRELPRKAVVWSFVVIAIVGTLAWTQNMLTAPIGSGWKYYGMLMSLA
                     LIVPFGLIFYNLISTLGSSDFHMRAPLRFALGALSLASIGLAAEIAQSTIAVATQLSE
                     TYDAWAATHFTLIGFVLFGGFAAVTYWYPKMTGHDLNEDRAAKSFGLMFVGTLIALLP
                     LFAAGVSGQVTDSYRYFSYEDLGVYNAISAVGTLLLFVGVVLAVGNLVKSRSEEPAAT
                     PDPYRGDSLEWLALSPPPAHNFDVLPDVRSTRPMRDIREAVIRRDVEDAEAESAAQPV
                     A"
     gene            12699..13643
                     /locus_tag="PLJ64_04790"
     CDS             12699..13643
                     /locus_tag="PLJ64_04790"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HNC92840.1"
                     /translation="MNSGADAHSVRTRQAAQPAQSGLQLDLIPMVRDYISLTKPRIIS
                     LLLLTTVATMFVADPSGPALSTILWTMLGGYLAAGGAGAINHYIDRNRDARNNRTKDR
                     PLADGRIKPINGLIFGIVLGVISFFQLWLTVNLLAAGLAMVGLFGYVFLYTLWLKPLT
                     PQNIVIGGSAGAVPPLVGWAAASGSLTWEAMWPFLIIFFWTPPHFWALSLMIKDDYAK
                     TGVPMLPVVKGEEATRQSILIYTIFMVGFTVGPYCTGLVGPFYLAASVVLGLIYIGFS
                     FMLWRRPSPKITLQSYLYSLAYLALLFISMAVAAVASA"
     gene            13852..14136
                     /locus_tag="PLJ64_04795"
     CDS             13852..14136
                     /locus_tag="PLJ64_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92841.1"
                     /translation="MSQITKPTETIQLPAPSFAPAVFALGVLGLIAGTFANDFMFPAW
                     FYAFVGFFFCLFSLRSMIKKGRRAFYALPREQGDARAELPVESFTAPTAD"
BASE COUNT         2372 a         4918 c         4483 g         2507 t
ORIGIN      
        1 atctccgcga aacaggagat acggcagccc gagacggtcg gcgctgatcg aggcgctcag
       61 ttcggtcgcg gtcaactcgt agggaccaat cgccacgacc cgattctcgc agggcttgcc
      121 gactccatcc cttgggaggt tttcgcggac cggccgtgcc gcttgagccg tgtcatggac
      181 gcatggacgc atcgggggcg tttgggcctg gcagcaatct gtctggggtt gtccgcactg
      241 gtcgcgcttc tggcggcact tccctccccc gccgtagccg cgccccctcc gcttccggat
      301 ctggtggtcg agtccggaac agctccggcc tcggccaccg ccggacggca ggtctcaatc
      361 aaagtgaagg tcaggaaccg cggcaggaag ccggcgcccg tctcccgggc gctggttctg
      421 ctcagccgcg acgcaaaacg cgacgtgacc gacacgaaac tcggcctggt caagatcagg
      481 aagctggccc ggaacaaggc tgtcgtgagc ggcggaaagt tgaccatccc ctccggccag
      541 cccgccggtt cccggcgcgt gattctttgc gcggatggcg accgcatgat cagggaatcg
      601 aaagagagca acaactgtcg caccctcgcg aacgcgatcc aggtgatgcc gggagggacc
      661 gggcctaccg gcccgacggg tccgaccgga ccgacctctc cgactggccc caccggaccg
      721 acctctccca cgggccccac gggaccaacc ggacccaccg gtccgacaac cggcttggcc
      781 ctctccccca gccccttgat tctgggctcg ctcagctact cgttcaccaa cgaagaatcc
      841 ttcgatgcga tcaggtacct gaccctgacc aacctgggac caggcgcttc ccagccgatc
      901 tcgatctggt cattgggcat gcatggcggc cagtacgccg acctgatggc catcggtgaa
      961 tcccacggag gtttgaacgg ctgcttcgag ggtcaatcgc tcgcggtggg agcccagtgc
     1021 tcgatccacg tttcctgggc tccccgcaac cgcagcacca ccgacatgac gggggtcgtc
     1081 gagatccggc tatggaacac gaccaccgtc ctggcttcgg tcccggtctc cgccgcctac
     1141 cagagccgtg cctacatcac aggcggtctc tctcccgcct cgcttaccgc caaccagggg
     1201 ttcaagcagc gaggtgatgc aaccctcacg aacgtcggag attcccctgc gacaccgagc
     1261 gtaagcgtct ccggtcccga tgcccagttc ttcgcggtcg actcgaaccg cgagaacacc
     1321 tgtgaagggt tctcgatcgc cgtcgacgcc acctgcaaac tcccgatcgt cttttgtgct
     1381 ggcgtcgtgc gtgactacga ggcgtctctt gtcgtcacgg tcaacaacgt ggtcaccgaa
     1441 tcgtcaccgc tgcgggcgac ggtcaggcag ctcaacgacc acccccaatg cacccacctt
     1501 caaacgaacg actgacccct gctcctctag gatcagggtg tggtcaagat ctacacgaag
     1561 aagggcgatg acgggacgac cggactctgg tacgggggcc gggtcgagaa gtcggggctg
     1621 aggaccgacg cctacggaac gcttgacgag gcctgctctg cattgggcgt ggcgcgggcg
     1681 atgtgcgacg acagccagga ggaactggcg gcagacatcc tcgcccttca gaacgagctc
     1741 ttcgtggccg gcgcggagct ggcgaccgca ccggaggcgg ccgggcgtct cgaagacgga
     1801 ataagccgga tcaccgccgg gatggtcgat gcgctcgatc ccgagatcga caagtacatg
     1861 gcccacgtga atctgccgcc caagtttgtg attcccggcg gtaaccggct ttcagcccag
     1921 ctcgacgtcg cccgcaccat cgtgcgccgg gccgagcggg ccgtggtccg gatccagctt
     1981 gccgacgagc ttgcctcgac cgaaattctc cgcttcctga accgggcctc ggaccttgtc
     2041 ttctcgatgg cgcggtatgc cgacgtcgac ttcccggacc tcttcgaggg ccgccgcaag
     2101 tcgggcgaag acgaggagta gcaaggtgga cctgggactg agggaccggg tctgcgtggt
     2161 gaccggggcc agtggcggga tcggcctcga agtgacccgg cagctctgcg acgagggcgc
     2221 gaaggtactg atggtttcgc gtgatcccga acagctcgcg gcgatgcggg aagtggtccg
     2281 ggccgagggc ggtgaagtcg aggtggtggt caaggacatc accaccgact gcgccgggca
     2341 gcacatcatc gccgccgcca ggcaggcctt cggcaaggtc gattgcctgg tcaacaacgc
     2401 cggggtagcc gagtggaagc cgctcgagga agcctcagag gaagactttc gattccagtt
     2461 cgagatgagc gttatcgcgc cgctgaacct gatgcgggca gcaatcccgg agatggccga
     2521 gcgcggctgg ggccgggtcg tcaacgtttc ctcgaccgcc ggcaagcgtc cctcctcgat
     2581 gatgcccgac tactcggtgg gcaaggcggc gatgctttcg ctttcccgtc agttcgccga
     2641 acggcatgca gccgagggcg tcatggtcaa cgcgatctgc cccggaccga ctgcctccga
     2701 cctctggatg gccgagggcg ggctgctcga ccagtcgaag gaagcggcgg gtcacgagag
     2761 ccgggaggaa gcgttgaagg cggccggcgg gaagcggccg atcggtcggc tcgccacgcc
     2821 cgaagagatt gcctcggcga ttgtcttcct ctgctcggag cgtgcgtcct atgtctcggg
     2881 agctgcttgg ggcgtggacg gcggcaccgt cccgatcatc atttaaaggg gcggcccgtt
     2941 cgggccgcaa attgaacccg ccctcccaca gaagaaatgg atggatggga gggctccgtt
     3001 aggtggtggg atcagttctg ggcggggagg gttacgaggg tgtgcatttt gtggggggcg
     3061 atgtggtggc ggccgccgag accttcgagc tcgatcagga acgcgcagcc gactacctcg
     3121 ccaccgagcc gctcgaccgc atcgatcttc gcccgggcag taccgccggt cgcgatcagg
     3181 tcatcgtgga ccagcaccct cgccccgttg aagatcgagt cggaatggac ttcaagcgag
     3241 tccgacccgt actcgagctg atactcgacc gcgatcgtct cggaggggag ccggccgggt
     3301 ttgcgggccg ggatgaaacc gatgtccagc gcctgggcca gggccccgcc gaggatgaac
     3361 ccgcgtgatt cagcggccag cagaaggtcc ggcttgtgcg gcgccgccca ctcggccagg
     3421 tcgctgaccg cgctccggca ggcagcggcg tcgagcagga gcggagtgat gtcgcggaag
     3481 aggatgcccg gctcgggaaa atcgggaatc gcccggatca gcgaggcgta gtcggtctgg
     3541 cttgccaagg cttcgcccta cttccgggtc gcggtgaacc tctggtccac ctggcagagt
     3601 ccgctgacga tcatccggcc gctcaccttg ttgcctctga ccttgccggt cagggttcgc
     3661 tggtcatcct tgaagctgac gaatctggac cccttgaaca cggctgagaa gaccccgtct
     3721 gccttgatcc ttgaactggc ggagggccgc tgggcggccg acgggtacct gaaggcgacc
     3781 agggtcgagc cgatcccgta acaggaaccg gccggattgc cctcgaagta ggtcacccgg
     3841 ttgttcctga tcttcaggac gaccttgacc ttgccctcat ccctgccgac ataggtcccg
     3901 ttcttcgcgg cgccggcgac cccgggcagg gcgagggaag cgaccacggc aagggccagg
     3961 agcagtccgg tacggggcat cgcgcggcag cttaccggcg gcgggtgcgg catgatcccg
     4021 ctgtggactt caccaccatc gaccttcccg gcctgcttct tgacaccgag tcggcggtgg
     4081 ccatgaaggc tcacggcagc cgggaagccg cggtactgat cccgatccgg aactggccgg
     4141 aagcgccgcg gctgatcctc accgagcgcc gcgccgacct gcgcaagcac gcgggcgaaa
     4201 tctcctttcc gggcggcacc agcgaacccg gggaccgcga cctcgaagaa acggccttgc
     4261 gggaatcgca cgaggagatc gaccttcacc ccgaccaggt cgaagtgatc ggagcggtgc
     4321 cgccggtcgg caccttcgca acctccttcc ggatccagcc gatcgtcggc ctcgttgccg
     4381 aagaagcgaa ggtggtcgcc aacccggacg aggtcgcgac catccttacc ttcggcttcg
     4441 acgagctgct cgactcctac gggatgcggc gtctggtccg ccgcggggtg ccgatccgca
     4501 cccccacctt cgagatggac cgtcacctga tctggggcgc gacggcccgc attctgaccg
     4561 acttctttca ccggatcggg gcaatgaagt gacccttcgc agggtcgcgc ccggcgcccg
     4621 gcggttcctc gaccttctga actgaggcgc tgcgcgaagc cgaccggcgg gaggcagccg
     4681 gggccggttc tatgctgctc ccgtggccga ggcgctcaag ttcagcatcg acggggagat
     4741 ccgcgaggcc ggcgaagcgt tcctgccgct gccggatgac gggctgctgc gtggcgacgg
     4801 tgtgttcgaa gtcgcccggg tctacggagg caaacccttc gcgctcgacc ttcacttcga
     4861 ccggatggag cgttcggcgg caacgatcga gttgcccctg gatcgccccg ggctggaacg
     4921 ggatgcgaag aacctgatcg cttcagtcga cgatccggac tttctgatcc ggctggtgca
     4981 gacccgggcc ggccgccgca tcctctcgat cgaagagttg ccggtctaca ccccgaccat
     5041 ctcgctggcc accgtcacct attcacccac cgtgatcctc aacggggtca agtcgctttc
     5101 ctacgccgcc aacatgcagg tgaccaggct ggcccgggcg gccggagcgg acgaggcgct
     5161 gttcgttcgc ccggacgggg tcgttctcga gctgccgact tcgacgatct tctgggcaac
     5221 cgacgacgga cggctgcgta cgccggccct ggaagccggg gtgctcgact cgatcacccg
     5281 ccgcaagatc gtcgagcggc tcgaagtcga gcagggcgag ttcccgctgg aagacctgct
     5341 cgctgcttcg gaagccttcg tcgcctcgac cacccgcgag gcccatccgg tctcccgggt
     5401 cgacgaccgg gaaatgccgc tcgttgacgg tgagcagacg agggccgccg ccgcggcatt
     5461 cgccgacgcc ctggccgaag acctcgaagc tcaatgacgg caacgccggc catcagcatc
     5521 cgcgacctgc gcgtttcgcg cggggggaac cttgtcctgc ctgacatctc ggtcgagatc
     5581 gagaagggat ccgtgaccgg gctgctcggc ccgagcggat gcgggaagac gacccttatg
     5641 cgttcgatcg tgggagtcca gatcgtggcc gggggcacgg ttgaagtgct cggccggccg
     5701 gccgggtcca gtgacctgcg caaccgggtc ggttacgtga cccaggcgcc ctcggtctac
     5761 ggggaccttt ccgtgggcga gaatctcgac tacttcgcga gagtggtcgg ggtctccccg
     5821 gaacgggtcg cggagaccat cgaaacggtc ggcctctccg ggttcaccgg aaggctggtc
     5881 tcctcgctct cgggagggca acgggcccgg gtttcgctgg ccagcgctct cctcaacaaa
     5941 cccgacctgc tggtgctcga cgagcccacg gtcggcctcg atcccctgct ccgggccgaa
     6001 ctctggaaga ccttccatga actggccgaa agtggcacca ccctgctggt ttccagtcac
     6061 gtcatggacg aggccggcgc ttgcgatcac ctgattctga ttcgcgacgg acgcctgctg
     6121 cgccacacga cgcccgaagc actgcgccgg gaaaccggcg agaacgacct cggccgcgcc
     6181 ttcctcaagg tgatcgagtc ggacgaggag gcggcgtgag tccgcgcatc accctggcca
     6241 ccggcctcag ggtcctgcag cagatccggc acgatccccg gacgatcgcc ctcctgctgc
     6301 tcgttccggc ccttctcctc accctgatga agtacgtgtt cgaaagcgaa cccagggtct
     6361 ttcagcagat cggcgctccg ctctgcggcc tgtttccctt catcgtcatg ttcctggtga
     6421 cctcgatcac gatgctgcgg gaacgaacca ccggaacgct tgagcggctg atgaccctgc
     6481 cgatgtcaaa gctcgacctg ctgctcggct acgcgctggc cttcgggctg ctcgcggcgg
     6541 cccaggcgct ggtcatctgc ctggtcggat tcggcctcct gggcctgaac gccccccacg
     6601 gcgtcctgat catcgtcggg ctggcagtct tgaatgccct gctcgggatg tcgctcgggc
     6661 tgctcgtcag cgcctttgcg aacaccgagt tccaggcggt ccagttcatg ccggcgttca
     6721 tctttcccca gctgctgctc tgcggactgt tcgtggcccg cgaccagatg gcgcctctgc
     6781 tcgaagcggt ctcctgggtc atgcccttca cctgggccta tgacgccctg gcccgggccg
     6841 tttcccctca gccgctcagc ggccgggtcg ccgtcgacgc cgcggtcgta ctcgcggtga
     6901 cgattggatc actgattttc ggcgccatga ccctccgccg gcggactgcc tagaaagtcg
     6961 cgtcgcggag gtaccactcg gcgtcgaggg cgcccttgca gccggagccg gcggcggtga
     7021 ccgcctggcg gtaggtgtgg tcaaccaggt cgccgatcgc gaagaccccg gccaggttgg
     7081 tcttggtcga ggcggcctcg gtcttcacat acccctcgtc gtcggtgtcg acctggccga
     7141 cgaccagttc ggaacgcggg atgtggccga tcgccacgaa gacgccgtct accggaatct
     7201 cctcgacctc gccgctctcg gaatgcttga ggcgaacctt ctccagggcc ccgtcttccc
     7261 cggcgacgaa ttcctccggc gcccaggggg tcttcaggga aatgttgggc aggctctcgg
     7321 cccgctcctg catgatcttc gaggcacgga actcatcccg gcgatgaacc aggtcgaggc
     7381 tcgatgcgaa cttggagatg aagaccgcgt cttccatcgc cgagtcgccg ccaccgatca
     7441 ccatggtcga gcggtccttg aagaaagctc cgtcgcagac cccgcaggtc gagacgccgc
     7501 ggccggccat ctccatctcg ccgggaatgc cgaggcgttt cggctcggcg cccatcgcga
     7561 ggatgaccgt cttcgcgtga tgaacctcgt caccgagata gaccttgtgg atgccaccgt
     7621 cggtgagctc gaccttgtcg acgtcatcgg tgacgaaacg ggtgccgaag cgttcggcct
     7681 gatcccggaa acgggtcatc atctccggcc ccatgatgcc ctcgggaaaa ccggggaagt
     7741 tctcgacgtc ggtcgtgttc tggagctggc cgccccactg gaaaccttcg aagacgagcg
     7801 gattcagctc ggcccgggcc gcgtagagcg cggccgtgta cccggccggc ccaccgccga
     7861 tgatgatgat gttttcgacc tcggtatcgc tcatttcagg ttcctggttt ctcgtccgtt
     7921 ttcattactt tactttctga agtaagtgta gcgttcttca gagaatctct tcgatcatgc
     7981 tcggccgcgg cggcctgtcg atcggcaggc cttccttctt ccagcgcttg accctgtttc
     8041 tcaactggaa gaaggcggcg acaccgggcg ggatcggcag ccagaacgcg accagacgat
     8101 agatcaggat cgccgcgaag acctgctcct ccggaaagcc gaagagcacg aaggtgccga
     8161 tcatgcccgc gtcaaccgcc ccgaccccgg ccggcacgaa gggaatcagg ttggcgacca
     8221 tcccgatgaa gaagcccatc acgaccacgc ccaggggtat cgcaatcccg aaggccttga
     8281 acgaggccca gaggatgccg atattggcgg cccagaaccc gaccgccccg accacggcga
     8341 gtccggcccg ggatggatga ctgaccagat ggagggcccc ccgggtcccg tcggcgacca
     8401 tcgccggcac ggtcgaaagc cgggcgagaa acttcgcccc cggatgccgc ctcgcgtagc
     8461 cttcgatccg ccgctcgatg tcggcaggga cgagggcaag ggccccgccc agcagcatca
     8521 cgatcagggc gatcgccgcc ggaacgaagg tcagcgacac ctcggtcgag ccgtggagga
     8581 ccccggtgcc gagcaggatc ccgaagacca ggaccgccag cgggtagaag agatagtgca
     8641 ggacgatgaa ggcgaccatg cgggtgccga cgtcccgccg ctccatcccg ccctgccgga
     8701 gcgcccagta ggtcagcacc acgccaccgg ccccgcccgc cgagaagagc agggtcgcgg
     8761 cgaaaccggc catgttgatt tcgtaggcct cccgccaggt gatcggaaag ctctcgccac
     8821 cgacgaccgc gcggaacagg gcaatgtagg tgatgaagga gaggacgcag aatccgatcg
     8881 cgattccgac gtacaccggt ttcgcctccc ccagcatccc gatcgcgtcg ccgaacccgg
     8941 caagctttgg cagcaggaag tagatcgcga cgatcagcag aacgatcagg accgcggtct
     9001 ggaccgcccc cttggtggtg aagaccggga ggccgtcacc ctcctcgact tcctcctcgg
     9061 aacgatccgg cgtttcggtc atccgccggc tgcgatcgct tccagtcgct ggaggcgtcc
     9121 gttgatcagc tcgacgaccg gccggacgat cggttcgccc ttgcgggcca gcaggaccac
     9181 gcccgccccg accagcagat cggtcacgta atgctcgccc agatatatga gggcgaaacc
     9241 gagtgaaagc gcgtaggtcc atcccagcgc gccctcgacc ttgccgccgg catctgaaag
     9301 caggatcgct gccatcactg ccgtggcgaa gtgcagcgag ggcatcgccg cccagggatt
     9361 ggagccgagg gtgccgaaga tccgggtcca ggccgggccc cacagcttct cgccgtactc
     9421 gagcatcacc cgcttgacgt gctcgtcggc gatctcgggg tcgatgtagc cgttctcggc
     9481 tgcccaccag ggcggcgcgg ttggaaccag gaagtacatc gcgcagccca tgtcgaagac
     9541 cgcggccagc tgccgggccg attcggggaa cttctccttg ttgcggaccg cgatccagaa
     9601 gatcgccgcg tagggctgaa tgaaccagaa ccagtgggtc caggcggcga agccggtcag
     9661 gtcgttgccg tagcggccct gatggagcca cttctgaagg cgtgcgttgg gcagctgccc
     9721 gcggccgatg aagcggtcga tccgtatcgg gtactcgacg taaagcctct cccgcaggcg
     9781 cttcggatcg tcgtagggca attcatggca gaccgcgaag gcccacatct gggctgcgaa
     9841 gaggccgagg tcacggcgac gggaacgggg ccacagaacg gcgatcgcca acggcgcgga
     9901 cacggttgaa gccacggtca ccgcggtcgg aatcctcagt tgccgccgca cgtggggaac
     9961 gatcaccgct gccgccagcg cgcctgcagc tgcgaggcga atgacggtcc gtttcgacga
    10021 ccgccggggc ttgaacgagg tcggcaatct catcccgggg tggaagcgta tgcgacggcg
    10081 cggcgcggcc tccgcaactg cgattagaat ggcccgcgtc ttgctgcctc gatccagaaa
    10141 acccgtgtct ctgttcatct ctctggccgg cgtcctgatc gcctttgcgc tgaacgcctc
    10201 gccggcgatg gcgctttcgc tcaatccgga cgccgcttcc cccgggatcg aggatgcgaa
    10261 cacgctgcat tggatcctct tcgtggtgat cctcgccgtg atcgtgctgg tcaacctggc
    10321 catcctccgg gccgcccggc cgcgcacccg cgcagtcagt ccgaagccgg gccggaaggt
    10381 ctcccaggtc aagctggcga tcggcctcgg ggttttcgcc ctcgccctct tcgtggtcgc
    10441 gacggtcttc tccgacaagt cccgcgagat tccggcagta aacgccgaac cggtcgccgg
    10501 gcaggtcaag ggcaagccgc tcgagatcac cgccaccggc tcccagtggc tgtggcgctt
    10561 caattatccg aacggcgcct tcagctaccg ccgcctggtg gtccctgccg gggtcaccgt
    10621 ccagctgaac ctggtctcga ccgatgtgat ccacggctgg aacgttcccg agctcaccgg
    10681 caaggcccag gccgttccgg gcaagaccaa cgaggtctac ttccgcgccg atgacccggg
    10741 cacctacacg agccgctcct cggtcttctc ggggcagggc tacgacacga tggaaatcga
    10801 agtccaggtc gtgcccaccg atgaatacga ggccgcgacc atcgccctga agaaggacat
    10861 ccaggacgcc caggacaagg tcgaagccga attcaaggcc agcaaggcga aggcggtcgt
    10921 ggccaccgcc gagggcaaga agatgaaggc cgaaatcaac aaagacgaca tcgaaagcgc
    10981 tgactcccaa tgagccgcac aaccagctac ccccagacga tgccgccgac ccggcccgag
    11041 atctcctctg acggtcccgg ctcacgccct tcgcgctggg ccgaactcgc ttccagcgcc
    11101 gatcacaagg acattggccg gctgctgatc gcaacctcct tcggttcgat cttcctcgcc
    11161 gcgctcgaac tgcttttgat gcgggttcag ctggcggtcc cggagaacgt cttcctcaag
    11221 ggagtcacct tcgaccgtct gctctccgtt tacggcgaga ccgcgatctt cctggtcgcc
    11281 ctgccgctga tcatcggcct gctctactac gtggttccgc ttcagatcgg cagccgcacc
    11341 accgcgatcc cgcgggtcgg ccagatcggt ctttggctgt tcatcttcgg tggcctcctg
    11401 ctttacggct cgttcctctt caccccgccg gaagccggca tcaacccgct tccccccttc
    11461 tccgacgcca ctttcacccc caacaacggc gttgacgtct ggatcggctc gaccgggctg
    11521 atcaccctcg gcctgctgat gatcgcgatc gacctgctcg ccaccctgca caacctgcgc
    11581 gcccccggca tggcctggcg ccgggcgccg ctcttcacct gggccggcgc gatcgtgacc
    11641 tggcttttcg tggtgatcgc cccgatcttc ctcgcggcca tcagcatgct gctgatcgac
    11701 cgcctctatg acggtggctt cttcgccggt gacgccggcg gctcgccgat cctctggcag
    11761 cacctcagct acatcttctt caccggcgtc tacatggccg tctcgatcac cttcctcgcg
    11821 gcgatcgggg agatcatcca gaccttcacc ggccgggagc tgcctcgcaa agccgtcgtc
    11881 tggtccttcg tggtcatcgc gatcgtcggc accctggcct ggacccagaa catgctgacc
    11941 gcaccgattg gcagcggctg gaagtactac gggatgttga tgtcgctggc cctgatcgtt
    12001 cccttcggcc tgatcttcta caacctgatc tcgaccctcg gctcgagcga cttccacatg
    12061 cgggcaccgc tgcgcttcgc gctcggcgcg ctttcgctgg cttcgatcgg gcttgccgcc
    12121 gagatcgccc agtcgaccat cgccgtcgcg acccagcttt cggagaccta cgacgcctgg
    12181 gccgccacgc acttcacgct gatcgggttc gtgctcttcg gcggcttcgc cgcggtcacc
    12241 tactggtacc cgaagatgac cggccacgat ctgaacgagg accgtgccgc caagtccttc
    12301 ggcctcatgt tcgtcggcac cctgatcgcc ctgctgccgc tgttcgcggc cggtgtctcg
    12361 ggccaggtca ccgactcgta ccgctacttc agctacgagg atctcggggt ctacaacgcg
    12421 atctccgccg tcggcacgct gctcctcttc gttggtgtcg tcctcgcggt cggcaacctg
    12481 gtcaagagcc gcagcgaaga gcctgccgcc acgcctgacc cgtaccgggg cgattcactc
    12541 gagtggctgg cgctctcgcc gcccccggcc cacaacttcg acgtcctgcc ggacgttcgc
    12601 agcacccgcc cgatgcgtga catccgcgag gcggtaatcc gccgcgatgt cgaggacgca
    12661 gaggccgaga gtgccgccca gccggtagcc tgaatgtcat gaactccggt gccgacgcgc
    12721 actcagtgcg tactcggcag gccgcgcagc ccgcgcagtc cggcctgcag cttgatctga
    12781 tcccgatggt tcgggattac atctcgctga ccaaaccgcg gatcatcagc ctgctgctgt
    12841 tgaccacggt ggcgacgatg ttcgtggctg acccatccgg ccccgccctc tccaccattc
    12901 tctggaccat gctcggcggt tatctggcgg ccggtggcgc cggagcgatc aaccactaca
    12961 tcgaccgcaa ccgcgacgcg cgcaacaacc ggaccaagga ccggcccctg gccgacggcc
    13021 ggatcaagcc gatcaacggg ctgatcttcg gcatcgtcct cggggtcatt tccttcttcc
    13081 agctctggct cacggtcaac ctgctcgcag ccggcctggc gatggtcggt ctgttcgggt
    13141 acgtctttct ctacacgctc tggctgaagc cgctgacccc gcagaacatc gtgatcggtg
    13201 gcagcgccgg ggccgttccg ccgctggtcg gctgggccgc cgcctcgggt tccctgacct
    13261 gggaggcgat gtggcccttc ctgatcatct tcttctggac cccgccccat ttctgggcac
    13321 tctcgctgat gatcaaggac gattatgcga agaccggcgt gccgatgctg ccggtggtca
    13381 agggcgagga agcgacccgc cagtcgatcc tgatctacac gatcttcatg gtcggattca
    13441 cggtcggtcc ctactgcacg gggctggttg gccccttcta cctggccgcc tcggtggttc
    13501 tcggcctgat ctacatcggc ttctcgttca tgctctggcg ccggccgagc ccgaagatca
    13561 cgctccagtc ctacctctac tcgctggcct acctcgccct gcttttcatc agcatggccg
    13621 tggcagccgt ggcttccgcg tgacctgacc gcagtcgcgc cggtcttcgc cggcgggact
    13681 ccgcttcagg cgaccggaac gccccccgaa ttgatagaaa taggtcccat ggatcgcaac
    13741 acttccaaga aaaacttcac cacgggcatg ctgctcggcg cgacttccgc gtccatcttc
    13801 gccctcactt tcgtctttgc ggtcctctac atcgcgaact agggacaact catgagccag
    13861 atcaccaagc cgaccgaaac gatccagctt cccgctccct ccttcgcccc ggcagtcttc
    13921 gccctcggcg tgctcggcct gatcgccggc accttcgcca acgacttcat gttcccggcc
    13981 tggttctacg ccttcgtcgg cttcttcttc tgcctcttct cgctgcgcag catgatcaag
    14041 aagggccgcc gcgccttcta cgccctgccc cgcgagcagg gtgacgcccg ggccgagctc
    14101 ccggtcgaga gcttcaccgc ccccacggct gactgacccc tccgggcagt actcgccccc
    14161 cgagtcggtc cggcccatcc ggggtcgatc cgcgaccccc gacccccgcg acatcccact
    14221 ttgtgccgca taccggcaca aacagggcat tcgacgattc caggtccacc tcacgctcgg
//
LOCUS       DAOQKN010000047        14240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_1990_length_14240_cov_5.696228, whole genome shotgun sequence.
ACCESSION   DAOQKN010000047 DAOQKN010000000
VERSION     DAOQKN010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 14240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14240
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1990_length_14240_cov_5.696228"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1896
                     /locus_tag="PLJ64_04800"
     CDS             <1..1896
                     /locus_tag="PLJ64_04800"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNC92842.1"
                     /translation="LMALVGLVMRLIQAETIDITSVWFYRLMTLHAAGMLAGALLSMM
                     GGLWFVVRAVVPELNFGRVIASWASIIAGVVLVLVAVLIGGFAAGWTFLYPLPFDSSG
                     QWSTWAAVTFIIGMGLVGVGFLIYCIDVLRATTQTYGGLTGALAIKWLRGKDPTGPPP
                     QVIAATAVSLQGIICSAVGMTIVMALLDHTVDGDVMLDALWAKNLTYFFGHTIANLII
                     YLAAGMIYVLVPLYAGRQWKLSKPLAIGWLFTICFVMTAYSHHLYMDFVQPGAPQMIS
                     LVASSAAALPVAVVTIYTGMMLIWGSRFRWTLTSILLYLGFAGWAIGGAGAVLDSLIP
                     INFKFHNTLWVPAHFHNYLMMGVGLWMMAMVSYLLERAAGRTASRRTTIWAPALMVAG
                     GYGFIFCWYVSGALGIPRRWAVHPIGTEGYSLVASIFVVIFLAGFTMLLIEFASLART
                     ALARRRTATAEVPLQTEAPAHPEPAPGAPEPMLRSPFGIGVTVGLAVIASFVLLPPIS
                     DASIATIGFHHLAHGVQFFLAAMLAVALASTPSVARRMTPRWTNFGLAIVVLIPAAML
                     LFMVPAIYSEIDNDNFLHASYHFGVLLLGFITGLGASMLGRVAGWAVLVSSIGMALMF
                     AAGVTGG"
     gene            1899..2363
                     /locus_tag="PLJ64_04805"
     CDS             1899..2363
                     /locus_tag="PLJ64_04805"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNC92843.1"
                     /translation="MEEEQQEQSGDGNGTGADEGGDRTLQAWLAGIAIGCLVVAAMIV
                     AYEIGKNNPDQEAAVKKPVAAAGSSGSPAPASPEASTFASTCGSCHTLAAADTDATVG
                     PNLDDLQPDSAMVMAAIENGGAGSGVMPAGLLSGEEAQAVADYVAKSAGSGK"
     gene            2425..3114
                     /locus_tag="PLJ64_04810"
     CDS             2425..3114
                     /locus_tag="PLJ64_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92844.1"
                     /translation="MILGLAALLFLIPAAPARAHSNSPHLFESVPDQITPAHLAHGIE
                     VEMIDHDANLRLVNKSGNEVIVMGYDGEPYARLAANGEVFLNQRSPAYFLNQDRYATT
                     PVEASADPEAPAKWVKQSDDGTLIWFDRRSHSMSDQVPAVVDDTSVSQPVRDYQIPMR
                     VNGEPAKLGGTLYWNGQSEFPMGIVAGLLIVTAMCAGLGFLGIEALRRAEPESHGASG
                     GEVAPSPGPPQ"
     gene            complement(3073..3408)
                     /locus_tag="PLJ64_04815"
     CDS             complement(3073..3408)
                     /locus_tag="PLJ64_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92845.1"
                     /translation="MSRQGAQCDWCGVELTEEMGYRLLWPDKSLGTAFCRLEHIVPFL
                     MQKDQWHIWKAVTVPADAPSLSTATGDELGENALYLVHHRGEHRITDSFENKEALLGW
                     ARAGGHFAP"
     gene            complement(3405..4253)
                     /locus_tag="PLJ64_04820"
     CDS             complement(3405..4253)
                     /locus_tag="PLJ64_04820"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC92846.1"
                     /translation="MAGKGISADGARPVRRFPWTRFVLPSYVFVVMAYTLVPIAVMIL
                     YTFNKAPNQRLTFSWHGFTTEWYSKLFEVQDLTQALWHSLEVAVISSIIATALGTPAA
                     IALARRRFRGRGAIDLVVVTNLTAPTVVVGASLLGFFIALSVPRGLVTILIAHVAFNI
                     AIVIIVVQARVSGFDESLEEAAADLGAPPWITFWKVTMPLILPGIFSAFLLSFALSID
                     DYIITSFVAGQTLTFPLWVAGAVKVGIPPQVFVMGTLIFVSGVLIALASLILQRWGDH
                     GETVRT"
     gene            complement(4253..5101)
                     /locus_tag="PLJ64_04825"
     CDS             complement(4253..5101)
                     /locus_tag="PLJ64_04825"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC92847.1"
                     /translation="MKSRIGPYLQSAPAMLWLALFFIVPLLAIVSISLMTGNSIDGYT
                     LTWNFSVFPDALDQYSAQLGRSFLYGGISTLLALIAMYPVAYWIAFHGGRRKSIFLFL
                     LLLPFLVSFIIRILTWQFILSDEGIVLGTLKDLGILGENAHILSTPVAVITGLTYDAL
                     PWMALPIYVALEKIDRDLVEAAGDLYASGYQQFRRIILPLSAPGIYAGILLVGIANIG
                     DYLSAQILGGPSTTMIGNIIQTQYVQNGDYPVASALSLMLMLALLLCVFVYARVFGTR
                     TIQEYA"
     gene            complement(5130..6380)
                     /locus_tag="PLJ64_04830"
     CDS             complement(5130..6380)
                     /locus_tag="PLJ64_04830"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HNC92848.1"
                     /translation="MGKEQRYIHRDPAWIRGMTQPRLSRRQVMKGAAGAAMSVSLAQL
                     IAACGLPGSRDTGWTAGTDWNAWWRDQQKNGSFNFASWPLYIDTDGGKHPSLDKFTKE
                     TGIDVTYSPVIQDNASFFSQISPVLQNGQSIGYDLIVISNGWQLTQLMQNKWLIPLDH
                     SRMPNFRKYAGTVATDLAYDPHLTYCAVWQAGVTAIAYDEKQVDGPIDSLDALFDPKY
                     KGKVGMLSDPDELGTAGLLALGINPQTSTYADWQKSADLLTRQRDDGIVRQYYDNSYI
                     KALEDGDLAISQAYSGDVFQAQNSGYPNLKFVIPKQGGVIWRDNMVIPYHAENPVDAI
                     EWINFAYNPEIAALIADWVNYICPVPAAKPIIANQLDDPTVANSPLVFPSEAELSRLK
                     EYPVTETLKTHEQWVGLFDPIIQS"
     gene            6565..7710
                     /locus_tag="PLJ64_04835"
     CDS             6565..7710
                     /locus_tag="PLJ64_04835"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92849.1"
                     /translation="MLEPIALAPQHDGPQTVGEVSPAAHRLGVRPGMGPGEAVDICPE
                     LVLITPDPTRASQIWEAVLGRIESTGAEVESVRDGEAFFDATGVELLHGGLEGVLDAV
                     SEKLGPNFLLAAAPTRLAALALAGRQPAGKSSGHGRVLPPEELAAFLDPLPLATLLDR
                     LPGPAGEVRRMLESMRRLGLGTLGDLVALPADAVADRFGPAGLAARDLALGLEGPIRP
                     RTPFEQITEWLELPDAASGIHLEGGLTILCDRLAGRMRAAGLSLRSLSVEARLLGGGS
                     WSHRASPRRPTTSSPVLRMLLFPALEQLPRPADRLGLRALETAPQAADQLEVIHRPGE
                     TRRRRLGEAARQVRAAVGESALMRILETEPESRLPERRMLLTPYSTE"
     gene            7713..7997
                     /locus_tag="PLJ64_04840"
     CDS             7713..7997
                     /locus_tag="PLJ64_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92850.1"
                     /translation="MSASMRRATMVTSGTRPTRLYEPRPVEVASDAAGAPVQINDVDV
                     VQVREEWIVEDGWWTAAPISRWYFEVVLIDGRDLAVFRAFPSGQWFAQRA"
     gene            8082..11402
                     /locus_tag="PLJ64_04845"
     CDS             8082..11402
                     /locus_tag="PLJ64_04845"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HNC92851.1"
                     /translation="MSSYVELHCHSSFSFLDGASSPLELAEKAAELGYPALALTDHDG
                     VWGSMEFAVACKGAGIRAITGTELTVEEGGRLFHLTLLVENETGYHNLCRLLTASHAH
                     TRDGRDRRVGQPRVPLGDLAGRAEGLICLTGCARQGLIPAAWADGDLARGETLARRLR
                     RWFTPENLRVEIQRPYWRHDHARNRWLAGLARSLELPLVATGNVHSHERERARLQDAL
                     VAVRLGSNLADSEPQRRGNSTSTLASPGQMAYRFPDYPEAVSETVRVAERLVFDLERD
                     LGYRYPQQGDPKTDRDLAALCQTRIDERYRGSRQEPEARARLENELKIIRGLGLSGFF
                     LLHHDLLELAREVAVEVRGTSSARGVLPPGRGRGSSVSSIVCYLTGLSHIDPVKADLF
                     PGRFLNEEVTTAPDIDLDFPRDIRERLIPRIHQRYGRDRSALVAAFPTYRSRGAIRDF
                     GKVLGIPDTEIERAARAVDFHSGSDDFQRDLTQAIGRERAHSAAWKHLALLSRQARSL
                     PRHASQHSGGMVIATRPLVEICPVVPAAMEQRQIVQWDKDSCQDAGFLKIDLLGLGML
                     SAVERTIDEIHRIRGETVDLSRIDLDDRAVFGMIKRAETTGVFQIESRAQMQMLPRMR
                     PDNIDDLTIQVALIRPGPIQGGAVHPYIERRRRLREDPDYEIPFAHELLRPVLGETHG
                     VIIFQEQVIEVAMHVAGFSSSEAESLRRAMSRKRSRRSLEAHHQRFIEGAMKSGVSRE
                     IADGIFEQIIGFSGFGFPKAHSAAFGLLAYQSAWLKHHYGPEYLASLLNEQPMGFYPP
                     DSLVQEARRTGVEVLPPDVNHSRVECVTEWPGSGGESSPRAESGPEGALARDEAATAR
                     RQQPAVRIGLGYVKGINESEMEKLVAERDRGGSFGDLGDLASRLPLARSDLEQLAWAG
                     ALRSLPRGERVAGLWSVGLTANRLPRAGGRQLALPFSQEETPELPEPKDWARVQAEYG
                     SIGMTLEGHPMELARRQLAESVLSTTDAASRPDRSWVEVAGLQVARQRPETAHGVIFI
                     LIEDEHGTLNLILPPPVAKRHRQVIRTATLIKANGRIETGQGVVNLVVRGLVEVHPPD
                     PRIKASAPVGINFGRRGR"
     gene            11603..12100
                     /locus_tag="PLJ64_04850"
     CDS             11603..12100
                     /locus_tag="PLJ64_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92852.1"
                     /translation="MFVQKEKKSSRVATRAADALDLMIEFATLGEYGLEYPEPVRSGS
                     RRNTTPDVRMERIARILEGSQPGDATGGSGFVDQERSAGHRRGSTPQKHRHGRRPCRP
                     GRTRVDLPGWGDRPGHEIPGVQPARRHKAAVRERTESRKAQRNRPTADFPEVLRPPRI
                     RLEAA"
     gene            complement(12101..13252)
                     /locus_tag="PLJ64_04855"
     CDS             complement(12101..13252)
                     /locus_tag="PLJ64_04855"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNC92853.1"
                     /translation="MPASEVEVDSSLIFALSEGIGPRPPTSNQEHVAAEVIAGEFEEL
                     GLSSKIEYFDSQRSFGPTYLVMFLIALLAGPLARRSRLAGTLAGLVATALGAAESGFA
                     LRSPLNLLRTRRSQNVWSVIEPESAPQQTICLVSHMDSSRSGLMFHPRFTPYLGRASF
                     AAGVALMLNGVAPLISWMPPGRLLVKAARGLIGLSALLVLERELRGEDVPGANDNASG
                     VAACLNLARQFDGDRLEHSRLVILVTGSEESGVIGMREFLRRNDTEGWIFINFDGVSA
                     DAPLRVLSREGGPTGSKADPDLMATAERVGRKHPELAAEPLLDGSGLPYDATAVNARG
                     GRAITIANQSGAILNYHWPTDSAANISEPAFAKAVRFAAALVEELDGEA"
     gene            complement(13400..13518)
                     /gene="rrf"
                     /locus_tag="PLJ64_04860"
     rRNA            complement(13400..13518)
                     /gene="rrf"
                     /locus_tag="PLJ64_04860"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(13584..>14240)
                     /locus_tag="PLJ64_04865"
     rRNA            complement(13584..>14240)
                     /locus_tag="PLJ64_04865"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         2435 a         4698 c         4620 g         2487 t
ORIGIN      
        1 ctgatggccc tggtcggact cgtcatgagg ctgatccagg ccgagacgat cgacattaca
       61 tccgtctggt tctatcggct gatgaccctt cacgcggccg gcatgctcgc cggggcgctg
      121 ctttcgatga tggggggtct ctggttcgtg gtccgggcgg tggtgccgga gctcaacttc
      181 gggcgggtaa tcgcgagctg ggcgagcatc atcgccgggg tcgtgctggt tctggttgcc
      241 gtcctgatcg gcggattcgc ggccggctgg accttcctgt accccctgcc cttcgattca
      301 tcggggcagt ggtcgacctg ggctgcggtc accttcatca tcggcatggg actggtcggg
      361 gtcggctttc tgatctattg catcgacgtc ctcagggcga ccacccagac ctacggcggc
      421 ctgaccggcg cgctcgccat caagtggctc cgcggcaagg atccgaccgg acctccgccc
      481 caggtcatcg ccgctaccgc agtgtcgctt cagggaatca tctgcagcgc ggtcgggatg
      541 acgatcgtga tggcgttgct ggaccacacg gtcgacggcg acgtgatgct cgatgcgctc
      601 tgggcaaaga acctgaccta cttcttcggc cacacgatcg ccaacctgat catctacctg
      661 gccgccggga tgatctacgt cctggtgccc ctctacgccg gccggcagtg gaagctctcg
      721 aagcctctgg cgatcggctg gctcttcacc atctgcttcg tcatgaccgc gtactcgcat
      781 cacctgtaca tggacttcgt acagcccggc gcgccgcaga tgatctcgct ggtcgcctcc
      841 tcggctgcag cactcccggt ggcggtggtg accatctaca ccggcatgat gctgatctgg
      901 gggtcccgtt tccgctggac gctcacttcc atcctgctct atctcggatt cgccggctgg
      961 gcgatcggcg gagccggagc ggtcctcgac tccctgatcc cgatcaactt caagttccac
     1021 aacaccctct gggtgccggc ccatttccac aactacctga tgatgggcgt cgggttgtgg
     1081 atgatggcga tggtctccta cctgcttgaa cgggccgccg ggaggaccgc cagccgccgg
     1141 accacgatct gggcgcccgc cctcatggtc gccggagggt acggattcat cttctgctgg
     1201 tacgtctccg gtgccctcgg cataccaagg cgctgggcgg ttcacccgat cgggaccgag
     1261 ggctacagcc tggtcgcctc gatcttcgtg gtgatcttcc tggccggttt cacaatgctg
     1321 ctgatcgagt tcgcttccct cgcccgtacc gcgctcgcgc ggcgtcgcac ggccacggcg
     1381 gaggtaccgc tccagactga agctccggcg catcccgagc ccgcacccgg tgccccggag
     1441 ccgatgctcc gttcgccctt cgggatcggg gtgacggtgg gccttgcggt gatcgcgtcc
     1501 ttcgttctgc tgcccccgat ctcggacgca tcgatcgcga ccattggttt ccaccacctg
     1561 gcgcacgggg tccagttctt cctggccgcg atgctggcag tggcgctcgc ctcaacaccc
     1621 tcggttgccc ggagaatgac gccccgctgg accaacttcg gcctcgccat cgtcgtgttg
     1681 atcccggccg cgatgctcct tttcatggtt cccgcgattt attcggagat tgacaacgac
     1741 aatttcctgc atgcctcgta tcactttggc gtcctgcttc tcggcttcat caccgggctc
     1801 ggcgcttcga tgctcggccg tgtagcgggc tgggccgtac ttgtctcgtc gatcggaatg
     1861 gcattgatgt tcgccgctgg cgtaactgga ggttgaagat ggaagaagaa cagcaagaac
     1921 agtccgggga cggaaacggg accggcgcgg acgaaggggg cgaccggacg cttcaggcct
     1981 ggctggccgg catcgcgatc ggctgcctgg tagttgcggc gatgatcgtc gcctacgaga
     2041 tcggcaagaa caaccccgat caggaggccg cggtcaagaa gccggtcgcc gccgcagggt
     2101 cctccggctc acctgccccg gccagtccgg aagccagcac cttcgcttcc acctgcggca
     2161 gctgccacac cctcgcggcg gccgacaccg acgccacggt cgggcccaat ctggatgacc
     2221 tccagcccga ctcggcgatg gtgatggcag ccatagaaaa cggcggcgcc ggctccggcg
     2281 tgatgccggc aggcctgctc tcgggtgagg aagcccaggc agtcgccgat tacgtggcca
     2341 agtcggcagg ttccgggaag tagcgccaaa gtggaccggc atcgacgagc atcgaaagcc
     2401 gggtccccga tcttcgtgcg agcgatgatc ctgggccttg ccgccctgct ctttctgatc
     2461 ccggcggcgc cggcccgcgc ccacagcaac tcgcctcatc tgttcgaatc ggtcccggac
     2521 cagatcaccc ccgcgcatct ggcccacggg atcgaagtcg aaatgatcga ccacgacgcg
     2581 aacctgcgcc tggtcaacaa gagcggcaat gaagtgatcg tcatgggcta cgacggcgag
     2641 ccgtacgccc ggctcgcggc gaacggagag gtcttcctca accagcggtc acccgcctat
     2701 ttcctcaacc aggaccgtta cgcgacgacc ccggtcgaag cttccgcaga tcccgaagct
     2761 ccggccaaat gggtgaagca gagtgatgac gggaccctga tctggttcga ccggcgcagc
     2821 cactcgatga gcgaccaggt ccccgcggtg gtcgatgaca cttccgtctc gcagcccgta
     2881 cgcgattacc agatccccat gcgtgtaaac ggtgagccgg ccaagcttgg aggaacgctc
     2941 tactggaacg gccagagcga gttcccgatg gggatcgtgg ccggcctgct gatcgtcacg
     3001 gcgatgtgcg ccggactcgg atttctgggc atcgaagccc tccgccgggc cgagccggaa
     3061 agccatggcg cctcaggggg cgaagtggcc cccagcccgg gcccacccca gtagcgcttc
     3121 cttgttctcg aacgagtcgg tgatcctgtg ttcaccccgg tggtggacca ggtagagggc
     3181 gttctctccc agttcgtcac cggtggcggt gctcagggac ggagcgtcgg cgggcaccgt
     3241 caccgccttc cagatgtgcc actggtcctt ctgcatcagg aacggcacga tgtgttcgag
     3301 gcggcagaag gcggtgccga gacttttgtc cggccacagc agccggtagc ccatctcctc
     3361 ggtcagctcc accccgcacc agtcgcattg cgcaccctgt ctgctcatgt cctcaccgtc
     3421 tctccatggt caccccagcg ttgcagaatc aggctggcca aggcaatcag gaccccgctg
     3481 acgaagatca gggtgcccat cacgaatacc tgtggcggga tgccgacctt gaccgcaccg
     3541 gccacccaca gggggaaagt cagggtctga ccggcgacga agctggtgat gatgtagtcg
     3601 tcgatcgaga gcgcgaagga gagcaggaag gcagagaaga tgccgggcag gatcagcggc
     3661 atcgtcacct tccagaaggt gatccagggt ggcgccccga ggtcggccgc ggcctcctcc
     3721 agggattcgt cgaaaccgct gaccctggcc tggacgacga tgatcacgat cgcgatgttg
     3781 aaggcgacat gggcgatcag gatcgtgacc agcccgcgcg gcaccgaaag ggcgatgaag
     3841 aagccgagca gcgacgctcc gaccaccacg gtcggggcgg tcaggttggt gaccaccacc
     3901 aggtcgatcg ccccgcgacc ccggaacctg cggcgggcca gggcgatcgc ggccggggtg
     3961 ccgagcgcgg tggcgatgat cgaactgatc accgctactt ccagtgagtg ccagagggcc
     4021 tgggtcaggt cctgaacctc gaagagcttc gagtaccact cggtcgtgaa gccatgccag
     4081 gagaaggtga gccgctggtt cggcgccttg ttgaaggtgt agaggatcat caccgcgatc
     4141 gggaccagcg tgtaggccat gaccacgaag acataggaag gcaggacgaa gcgggtccag
     4201 gggaagcggc ggaccgggcg ggcgccgtcg gcggagatgc ccttgccggc catcaggcgt
     4261 actcctggat ggtccgggtg ccgaagaccc gggcgtagac gaagacgcaa agcaagagcg
     4321 ccagcatcag catcagcgac aaggccgagg cgaccgggta gtcaccgttc tgcacgtact
     4381 gggtctggat gatgttgccg atcatcgtgg tgctcggacc gccgaggatc tgggcgctca
     4441 ggtaatcgcc gatgttggcg atcccgacca gcaggatgcc ggcgtagatg cccggggccg
     4501 agagcggcag gatgatccgt cggaactgct ggtagccgga ggcgtatagg tcaccggccg
     4561 cttcgaccag gtcgcggtcg atcttctcca gggcgacgta gatgggcagc gccatccagg
     4621 gcagcgcgtc ataggtgagt ccggtgatca ccgcgaccgg agtcgaaagg atgtgggcgt
     4681 tctcgccaag gatcccgagg tccttcaggg tgccgagcac gatcccctcg tcggaaagga
     4741 tgaactgcca ggtcaggatg cggatgatga aactgaccag gaagggcagg agcagaagga
     4801 agaggaagat cgacttgcgc ctgcccccgt gaaaggcgat ccagtaggcg accggataca
     4861 tcgcgatcag ggccagcagg gtcgagatcc cgccgtagag gaaggagcgg ccgagctggg
     4921 ccgagtactg gtcgagcgcg tcgggaaaga ccgagaagtt ccaggtcagg gtgtagccgt
     4981 cgatcgaatt gccggtcatc agcgaaatcg agacgatcgc cagcagcggc acgatgaaga
     5041 agagcgccag ccagagcatg gccggtgccg actgcaggta cggcccgatc cgggacttca
     5101 ctgtgacctc cgcttgctgg ccggggcgct catgactgga tgatcgggtc gaagagtcca
     5161 acccattgct catgggtctt gagagtctcg gtcaccgggt attccttgag tcgggaaagc
     5221 tcggcttcgg atggaaagac gagcgggctg ttggccacgg tcggatcgtc gagctgattg
     5281 gcgatgatcg gcttggcggc cggcaccggg cagatgtaat tgacccagtc cgcgatcagg
     5341 gctgcgatct ccgggttgta ggcgaagttg atccactcga tcgcgtcgac cgggttttcg
     5401 gcgtggtacg ggatcaccat gttgtcccgc cagatcaccc cgccctgctt cgggatcacg
     5461 aacttgaggt tgggatagcc ggagttctgg gcctggaaga cgtcccccga ataggcctgg
     5521 ctgatcgcca ggtcgccatc ttcgagggcc ttgatgtagc tgttgtcgta gtactggcgg
     5581 acgatcccgt cgtcgcgctg tctggtcagc aggtcggcgg acttctgcca gtccgcgtag
     5641 gtcgaggtct gcggatttat gccgagagcg agcaggccgg ccgtgccgag ctcgtccggg
     5701 tcgctgagca tcccgacctt acccttgtac ttcgggtcga agagggcgtc gagggaatcg
     5761 atcggaccgt cgacctgctt ctcgtcgtag gcgatcgccg ttaccccggc ctgccagacc
     5821 gcgcagtagg tcaggtgcgg gtcgtaggcc aggtcggtgg cgaccgtccc ggcgtacttc
     5881 ctgaagttcg gcatccgcga atggtcgagc gggatcagcc acttgttctg catcagctgg
     5941 gtcagctgcc agccgttcga gatcacgatc aggtcgtagc cgatcgactg gccgttctgc
     6001 aggaccgggc tgatctggga gaagaaggaa gcgttgtcct ggattaccgg gctgtaggtg
     6061 acgtcgattc ccgtttcctt cgtgaatttg tcgagcgaag gatgcttgcc gccgtcggtg
     6121 tcgatgtaaa gcggccagct ggcgaagttg aaggagccgt tcttctgctg gtcccgccac
     6181 caggcgttcc agtcggtgcc ggccgtccag ccggtgtcgc ggctgccggg cagcccgcag
     6241 gcggcaatca gctgggcgag gctgacgctc atcgcggcgc cggccgcgcc cttcatgacc
     6301 tgccggcggg aaagcctggg ctgagtcatc cccctgatcc aggccggatc acgatggatg
     6361 tatctctgct ccttgcccac ggattcacga tagcgatccg ctttcgcgcc ccggtcggcc
     6421 gaaaagtccg gaaagtcccc tttcgtatga cgatctgctt gacctggccc ggccggatat
     6481 gcgaacatat gttcgtatat gttcgtttgt gtcctgcttc cccttctggc tctccgggca
     6541 gccctcgaaa agggtcaaaa ccgcttgctg gagccgatcg ccctggcccc gcagcatgat
     6601 ggcccgcaga cggtcggcga ggtttccccg gccgcacacc ggctcggagt aaggcccggc
     6661 atgggtcccg gcgaggcagt tgacatctgt ccggaacttg tcctgatcac tcccgatccg
     6721 acccgggcca gccagatctg ggaggccgtc ctggggcgaa tcgaatcgac cggtgcggag
     6781 gtcgaatcgg tccgggatgg cgaagccttc ttcgacgcca ccggtgtcga gctgctccac
     6841 ggcgggctcg aaggcgtact cgatgcggtc agcgaaaagc tcgggccgaa cttcctcctc
     6901 gccgccgctc cgacccgtct ggccgccctc gccctggccg gcaggcaacc ggccggcaag
     6961 agttccggtc atggccgggt cctgccgccg gaggagcttg ccgcctttct cgacccgctc
     7021 cctctcgcga ccctgctcga ccgcctgccc gggccggccg gcgaggttcg ccggatgctc
     7081 gaatccatgc gccggctggg gctgggaacc ctgggggatc tcgtcgccct gccggctgac
     7141 gcggtcgccg accgtttcgg ccccgccggg ctggccgccc gtgacctggc cctcggcctg
     7201 gaagggccga ttcgccctcg cacccccttc gagcagatca ccgaatggct ggagctgccc
     7261 gacgcggcca gcggcatcca cctcgaaggc gggctgacca ttctttgcga tcgcctggcc
     7321 gggaggatgc gggcggcggg cctcagcctg aggtcgcttt cggtcgaggc ccggctgctc
     7381 ggcggcggca gctggtcgca ccgggcctcg ccccgccgcc ccacgacctc ctcgccggtt
     7441 ttgcggatgc tcctcttccc cgctctcgag cagcttcccc gcccggccga ccggctcggc
     7501 ctgcgggcgc tggagaccgc tccccaggcg gccgatcagc tcgaggtcat ccaccgtccc
     7561 ggcgaaaccc gtcgccgccg gctcggtgaa gcggcccgtc aggtccgcgc cgcggtcggg
     7621 gaaagcgccc tgatgcggat cctggaaacc gagcccgagt cccgcctgcc cgagcggcgc
     7681 atgctcctga ccccctactc gaccgagtag cggtgagcgc ttcgatgaga agagcgacca
     7741 tggtcacttc gggcacccgg ccgacccggc tttacgagcc gcgaccggtc gaggtggcca
     7801 gcgacgcggc gggggctccg gtccagatca acgacgtgga cgtggtccag gtccgggagg
     7861 aatggatcgt cgaggacggc tggtggaccg ccgccccgat cagccgctgg tatttcgagg
     7921 tggtgctgat cgacggccgt gacctggccg tcttccgcgc cttcccctcc ggccagtggt
     7981 tcgcccagcg ggcctgagga acgagatggc aaaagagagc gctatatggc cacttttgcc
     8041 atctcggtgc ccctcccgac ctcgccgggc ccgaggatga catgtcgtcg tatgtcgagc
     8101 tgcattgcca ttcgtctttc tcctttctgg acggggcttc ttcgccgctg gaactggcag
     8161 agaaggcggc cgaactgggt tacccggccc tggccctgac cgatcacgac ggggtctggg
     8221 ggtcaatgga gtttgcggtc gcctgcaagg gcgcggggat ccgggcgatc accggaaccg
     8281 agttgaccgt cgaggaaggc ggccggctct tccacctgac cctgctggtt gaaaacgaga
     8341 ccggctacca caacctctgc cggctcctca ccgcctccca cgcccatacc cgggacggaa
     8401 gggaccggcg ggtcggccag ccccgggtgc cgcttggtga tctggccggc cgggccgaag
     8461 ggctgatctg cctgaccggc tgcgcgaggc aggggctgat cccggcggcc tgggccgatg
     8521 gtgacctggc ccggggcgaa accctcgcca ggcggctccg tcgctggttc actccggaaa
     8581 acctgcgggt cgaaatccag cggccctact ggcggcatga ccatgcccgc aaccgctggc
     8641 tggccgggct ggcccgcagc ctggagctgc cgctggttgc gaccggcaac gtgcacagtc
     8701 acgaacggga acgggcccga ctgcaggacg ccctggtcgc cgtccggctg ggctcgaacc
     8761 tggccgactc cgagcctcag cggcggggca actccacctc caccctcgcc tcgcccggtc
     8821 agatggcgta ccgctttccg gattacccgg aggcggtcag cgagacggtc cgggtcgcgg
     8881 agcgactcgt tttcgatctc gaacgcgacc tcggttaccg ctacccgcag cagggcgacc
     8941 cgaagaccga ccgggatctc gcggcgctct gccagacccg gatcgacgag cgctaccggg
     9001 gttcccggca ggagccggag gcccgggccc gtctcgagaa cgagctgaag atcatccgcg
     9061 gcctcgggct ctccggcttc ttcctgctcc atcacgacct gctggagctg gcccgcgagg
     9121 tggcggtcga ggtgcgtggc acctcctccg cccggggcgt gctgccgccg ggccggggac
     9181 gcggttcctc ggtcagctcg atcgtctgtt acctgaccgg cctctcccac atcgacccgg
     9241 tcaaggccga cctcttcccc ggccgtttcc tcaacgaaga ggtgaccacg gcccctgaca
     9301 ttgatctcga cttcccccgg gacatcaggg agcggctgat cccccgcatc catcaaaggt
     9361 acggccggga ccgctccgcc ctggtcgctg cctttcccac ctatcgctcc cgcggggcga
     9421 tccgcgactt cggcaaagtc ctcggcatcc ccgacacgga aatcgaacga gccgcgcggg
     9481 cggtggactt tcacagcggc tccgacgatt ttcagcgtga cctgacccag gcgattggcc
     9541 gcgaaagggc ccactcggcg gcctggaagc atctcgccct gctttcccgc caggcccgca
     9601 gcctgccccg tcacgcttca cagcattcgg gcgggatggt gatcgcgacc cggccgctgg
     9661 tcgagatctg cccggtcgta ccggcggcga tggagcagcg ccagatcgtc cagtgggaca
     9721 aggattcctg ccaggacgcg ggtttcctca agatcgacct gctcgggctg ggcatgctct
     9781 cggcggtcga gcggacgatc gacgagatcc accggatccg gggtgaaacc gtggacctga
     9841 gccggatcga cctcgatgac cgggcggtct tcggaatgat caagcgggcc gagaccaccg
     9901 gtgtcttcca gattgaaagc cgggcccaga tgcagatgct gccccggatg cgcccggaca
     9961 acatcgatga cctgaccatc caggtggcac tgatccgtcc cgggccaatt cagggcggcg
    10021 ccgttcaccc atatatagag aggcggcgaa ggctgaggga agacccggat tacgagatcc
    10081 cgttcgccca tgagctgctc aggccggtgc tcggcgagac ccacggggtg atcatcttcc
    10141 aggaacaggt gatcgaggta gcgatgcatg tggccggctt cagttcctca gaggccgagt
    10201 cgcttagaag ggcgatgagc cgcaagcgct cacgccgctc gctggaggcc catcatcagc
    10261 gtttcatcga gggcgcgatg aagagcggcg tcagccgcga gatcgccgac ggaatcttcg
    10321 agcagatcat cggcttctcc ggcttcggct ttcccaaggc tcattccgcc gccttcggcc
    10381 tgctggccta ccagtcggcc tggctcaagc accactacgg accggagtac ctggcttcgc
    10441 ttctgaacga gcagccgatg ggcttctacc cgccggattc actggtccag gaagcccggc
    10501 gcaccggggt cgaggttttg ccgcccgacg tgaaccacag ccgggtcgaa tgcgtcaccg
    10561 aatggccggg gtcgggcggg gaaagctctc cgcgggcaga gtccggcccg gaaggtgccc
    10621 tggcccggga cgaagccgca acagcccggc ggcagcagcc cgccgtgagg atcggtctcg
    10681 gttatgtgaa ggggatcaac gagagcgaga tggagaagct ggtcgccgag cgtgaccgcg
    10741 gtggcagctt cggcgacctc ggcgacctcg cctcccgcct gccactcgcg cgaagcgatc
    10801 tcgagcagct ggcctgggcc ggggcactca ggtccctgcc ccgtggtgag cgggtggccg
    10861 gactgtggag cgtcggactc acggcgaacc ggttgccgcg ggccggcggg cggcaactcg
    10921 cgctgccgtt cagtcaggag gagaccccgg aactcccgga accgaaggac tgggcgcggg
    10981 tccaggccga atacggctcg atcggcatga ccctggaggg acatccgatg gagctggccc
    11041 gtcgtcaact ggccgaaagc gtgctgtcca cgaccgacgc cgcctcccgg ccggatcgca
    11101 gctgggtcga ggtggccggt ctccaggttg cccgccagcg accggaaacc gcccacggag
    11161 tgatcttcat cctgatcgag gacgaacacg gcaccctcaa cctgatcctg ccgccaccgg
    11221 tggccaaacg acatcgccag gtgatccgca ccgccaccct gatcaaggcg aacgggcgca
    11281 tcgagaccgg tcagggggtg gtcaacctgg tcgtgcgcgg cctggttgaa gttcatccgc
    11341 ccgacccccg gatcaaggcc agcgcaccgg tcgggatcaa cttcgggcgg cgcggccggt
    11401 gagcgccgga aacgggcccg aacgagcgtt tcgggatgcc gcaaaatgcg aacgaaaaac
    11461 aaacgcctgt tcgtgcaggc ggccgaccga cagcccgaaa ccacggtttg cacttggctc
    11521 tgccaaacgg tttccgaacg attctgcacg gttaattgca aacatgtgtt cgtgtccgac
    11581 cgggcccgta cattacgaac ctatgttcgt gcagaaagag aagaagagca gccgggtagc
    11641 aacccgggcg gctgatgctc tcgacctgat gatcgagttc gccaccctcg gcgaatacgg
    11701 gctcgagtac ccggaaccgg tcaggtcggg cagccgcagg aacaccaccc ccgacgtccg
    11761 catggagcgc atcgcacgca tcctcgaggg cagccagcca ggcgacgcca ccggaggctc
    11821 cggattcgtc gaccaggagc gttcggccgg ccaccgccgt ggctccaccc cccagaagca
    11881 tcgtcacggc cgacgcccat gccggcccgg gcggacccgg gtcgacctcc ccggctgggg
    11941 tgatcgcccc gggcatgaaa tccccggcgt ccagcccgcc cgccgtcaca aggccgccgt
    12001 cagggagcgc accgagtccc gaaaggctca gcgcaaccga cctacggcgg acttcccgga
    12061 agttctgcgt ccgccgcgga tccggctcga agctgcctga tcaggcttcg ccgtcaagtt
    12121 cttcgaccag agccgcagcg aatcggacgg ccttcgcgaa ggccggttcc gagatgttcg
    12181 ctgcggagtc ggttggccag tggtaattga ggatggcgcc cgattgattg gcgatggtga
    12241 tcgccctgcc accccgggca ttgactgccg tggcgtcgta gggaagcccc gagccatcca
    12301 gtagcggctc ggccgcgagc tccggatgct tccggcccac cctctccgcg gtggccatca
    12361 gatccggatc cgcctttgag ccagtcggtc cgccttctct cgagaggacc ctgagcggag
    12421 cgtcggcact gacgccgtcg aagttgatga agatccatcc ctcggtgtcg ttccttctga
    12481 ggaattcacg catgccgatg actcctgact cctcgctgcc ggtcaccagg atcacgaggc
    12541 ggctgtgctc gagccgatcc ccgtcgaatt gcctggcgag gttgaggcag gccgccaccc
    12601 cggaagcgtt gtcgttcgct ccgggaacgt cctctccccg cagttcccgt tcgagaacca
    12661 gaagcgcgga aagcccgatc aatcccctcg ccgccttgac cagcaaccgg ccgggcggca
    12721 tccagctgat caggggagcg actccgttca gcatcagggc gacgccggcc gcaaatgacg
    12781 ccctgcccag atatggcgtg aacctcggat gaaacatcaa tccggaccgg gaagagtcca
    12841 tgtgggagac gaggcagatc gtctgttgcg gtgccgattc cggttcgatc acgctccaga
    12901 cgttctggct tctgcgggtt cgaagcaggt tcagcgggct ccgcagcgcg aaacctgact
    12961 ctgccgcgcc gagcgcggtt gccacaaggc ctgccagggt cccggccagg cgggatcggc
    13021 gtgccagcgg cccggccagc agggcaatca ggaacatgac cagataggtg gggccaaagc
    13081 tccgttgcga gtcaaagtac tcgatcttcg agctgagacc gagctcttcg aattcaccag
    13141 cgatcacctc ggccgcgaca tgctcctgat tgctggtcgg cggtctcggg ccgatcccct
    13201 cgctcagggc gaagatcagg ctgctgtcga cttccacctc gctcgcgggc acagggccag
    13261 actactcacg aaagaagcct gaatgccctt cggcagcgaa atcgtggtga cgagcggtgc
    13321 cgtcttcgtg aacaccctcg ccggccgccc ggatgcaaca aaaaaagccg ccccctgagg
    13381 gacggctttt ccaaagaaga accggcggcg acctactctc ccagggggtt gccccccaag
    13441 taccatcggc gctgtggtgc ttaactgctc tgttcggaat gggaagaggt gtttccacca
    13501 cgctattaca ccaccggaaa tatgtcgaga ctatgagcct cgataaagct tgaaaaccgc
    13561 atatcgaccg attgaaataa atatccgtca agccctcgac ccattagtac cggtcagctg
    13621 caggtgtcac cacccttcca ccgccggcct atcaacctgg tggtctacca ggggtcttac
    13681 tccttctaga ggatgggaga attaatctcg aggacggctt cccgcttaga tgccttcagc
    13741 ggttatccgt tccgtgcgta gctaagctgc ggtgcccttg gcaggacaac agctacacca
    13801 gaggcacgtt caccccggtc ctctcgtact aggggcaaat cctctcaatt ctccaacgcc
    13861 cacatcagat agggaccgaa ctgtctcacg acgttctgaa cccagctcgc gtgccgcttt
    13921 aatgggcgaa cagcccaacc cttgggacgt actcccgccc caggatgcga cgagccgaca
    13981 tcgaggtgcc aaaccgcttc gtcgatatgg actcttggaa gcgataagcc tgttatcccc
    14041 ggagtacctt ttatccgttg agcgacggcg cttccacttg caaccgccgg atcactaagc
    14101 ccaactttcg ttcctgctcg acccgtcggt ctcgcagtca agctcccttg ttgcctttac
    14161 actctacgca cgatttccaa ccgtgctgag ggaacctttg ggcgcctccg ttacatttta
    14221 ggaggcgacc gccccagtca
//
LOCUS       DAOQKN010000048        14042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_2026_length_14042_cov_6.235719, whole genome shotgun sequence.
ACCESSION   DAOQKN010000048 DAOQKN010000000
VERSION     DAOQKN010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 14042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14042
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2026_length_14042_cov_6.235719"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..868)
                     /locus_tag="PLJ64_04870"
     CDS             complement(170..868)
                     /locus_tag="PLJ64_04870"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC92854.1"
                     /translation="MPQEQVQEEQDPFGSGKLPSGRHGLPRDVVVRSQRERMVEAMIG
                     VVAEKGYTEATVADIISTAGVSRATFYEQFADKEDCFIAAYGTVMERMLTFVADGFAQ
                     GSSDDWVVQIRGGIGALLTYLAENPVAARVGIVEGFGAGPRARDRYQQAVSSFFPFLD
                     SARNMVDEPDRIPTQVARVIVGGVSALMFNEASNGRAADLPKMLPDMMYLAVTPYFGH
                     DRGIEAMEETRVSA"
     gene            983..2260
                     /locus_tag="PLJ64_04875"
     CDS             983..2260
                     /locus_tag="PLJ64_04875"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNC92855.1"
                     /translation="MNDLPSTGGPVGIVGLGYVGLPIAVAFAEAGRKVIGLDNDPAKI
                     EKINRAESYIEDIPSATLAALEDRLVATASYEDLAGCEAVIICVPTPLTGSREPDLSY
                     LESAAESLAGVLAPGQTVILESTTCPGTTRDLLARTLESGGGKSGEDFHLAFSPERID
                     PGRTDFTVRTTPKLVGGLTPACTDKAVALYETICDRVIPLSGPEAAELSKLLENIFRS
                     VNIALVNELAQLCDRLGVDVWEVIDAASTKPFGFMRFEPGPGMGGHCLPVDPFYLAFK
                     AREHDFYPEFIELAGKINQSQPLFCVNRIERVLNANGKPVKGTKVLILGVSYKAGVGD
                     TRESPAIKIIRLLGELGADVRYHDPHVPALKEAGLTNTPDLDGALADADLSVIITAHP
                     EFDWAEVIGKSDLVFDLRGVTREFDDPGNVTRL"
     gene            2287..3033
                     /locus_tag="PLJ64_04880"
     CDS             2287..3033
                     /locus_tag="PLJ64_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92856.1"
                     /translation="MIRELKGAASSLIPWRRAPHSHWQTIKSDRATLALAVGTVTIAG
                     LVLAAQYSRLLSRRTHSPGTEGLIDSAPAAAVDTVGVAVEGYSATPNRELILFNLLTG
                     FLGSFSAVRMTTWAIREGWGPFRNVSIGGRHIHHFVPGILIGFGSGIAALLLNGEKAD
                     RRIARSLGVGMGLTFDEAALLLDMQDVYWTRQGLLSVQVTLATGATLGATILTMRILG
                     RGETRQEEAGEIPAPEGQMNTVVPWPHPAM"
     gene            complement(3521..4852)
                     /locus_tag="PLJ64_04885"
     CDS             complement(3521..4852)
                     /locus_tag="PLJ64_04885"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HNC92857.1"
                     /translation="MPGLVIVGTQWGDEGKGKVTDLLAKDASAIVRFQGGNNAGHTIV
                     HDGEEYKFHLIPSGILHEDKYCVIGNGVVVDPRVLLEEIEGLHRAGVSASNLRVSSNA
                     HLIMPYHVLVDAAEELKLGKLSIGTTRRGIGPAYADKAYRIGIRVQDLLDEKILRKKI
                     MAALEHKKKSLQELAVRRRKLHKEAEPDEEIQIDDRLDLHTITEHYLSYGHRLEPYIA
                     DTARLCWNELDAGNKVIFEGAQAALLDLDHGTYPFVTSSNPIAGAACVGAGVGPTDIE
                     EVWGIVKAYSTRVGSGPFPTELFDEVGEYMSDKGHEFGTTTGRRRRCGWLDLVALKYA
                     VRLNRLSHLAMTKLDVLTGLDTIRVAVRYRSKEGALLEDFPYHQSILHNATPEYVDLP
                     GFSEDITGCRTEEDLPPAAREYISFISDYVGVPINLVGVGPARDEVVWIQS"
     gene            complement(4871..5608)
                     /locus_tag="PLJ64_04890"
     CDS             complement(4871..5608)
                     /locus_tag="PLJ64_04890"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC92858.1"
                     /translation="MAAGEFEASGNGQPPCPYGACDGNGWILNEDGSATECRCRQPRL
                     AKARASGVNSVLPKRYRGVSFDRPPVTEIHPGAVTVVRDWIERLDENLDQGNGLWLMG
                     DTGTGKTTLAMLVSKEALRRDHTVAIYSMPSLLTRIRATYGAEAGEESYDQFFERLCE
                     VDLLHIDDLGAEKQTEWVLEQLYALVNERYEREKSIVVTTNITDQGELEQQIGPRTVS
                     RLTEMCGESVVPLFGEDARQKPQLRDG"
     gene            complement(5608..7029)
                     /gene="dnaB"
                     /locus_tag="PLJ64_04895"
     CDS             complement(5608..7029)
                     /gene="dnaB"
                     /locus_tag="PLJ64_04895"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNC92859.1"
                     /translation="MAQVPPNNVEAEEAVLGSMLVYEPAVNWAVDEVKLKPGDFYLDR
                     HQAIYEAMIDLYAADKACDTLTVTEALVTSGRLDEAGGENYVAELGEKVAAPANARHH
                     AEIVREHAHLRRLLRAAQDIEVWVNDREADAGTLSERAEAELFKVAHRDQEGDFRSLE
                     NMLFEEHQRLEKRISGELKEVGTNTGFPDLDEKTAGFQPSNLIVLAARPSVGKSAFVA
                     NIAENVAVGQDKSVAFFSLEMSEGELSQRFIASHSGIHGDILRKARFKGGDRTWKKIN
                     KSLNALANVPLFVDVTSDLGLLDLRAKARRLHEQEIRRRAGTDRENEGLSLIIVDYLQ
                     LMRMDDRIANRAEQVGQVSRGLKLLASELKVPVVALSQLNRANESRTDKRPMLSDLRE
                     SGNIEQDADLVLFIYREDVYQKDREKHTGKAEVIVAKNRSGPIGTSHLIFFPHIPKFA
                     EESREHMAGEGSPQAVQAEADAI"
     gene            complement(7026..7478)
                     /gene="rplI"
                     /locus_tag="PLJ64_04900"
     CDS             complement(7026..7478)
                     /gene="rplI"
                     /locus_tag="PLJ64_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNC92860.1"
                     /translation="MAQAILLADVEGLGEHGTAVEVSPGYLRNYLQPRGLAQPATPGA
                     LEEAIRRKEAAEKAEREAIARAAENASLLSKTVLTINHRAGDDGKLYGSVTTKEIAEA
                     IRDARGIKVDRKKIRLDVPIHQVGTYMIDVEVAGGAIASVKTIVAEEK"
     gene            complement(7478..7735)
                     /gene="rpsR"
                     /locus_tag="PLJ64_04905"
     CDS             complement(7478..7735)
                     /gene="rpsR"
                     /locus_tag="PLJ64_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005705387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNC92861.1"
                     /translation="MAKTREASSRRGRRFSERVRPRKYTKISAENIDYKDLTLLRRFI
                     SDKGKTRSRRVTGLSRKHQAQLSVAVKRAREVALLPYVGEK"
     gene            complement(8087..8605)
                     /gene="ssb"
                     /locus_tag="PLJ64_04910"
     CDS             complement(8087..8605)
                     /gene="ssb"
                     /locus_tag="PLJ64_04910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNC92862.1"
                     /translation="MAENINRVTITGNLTRDPELRQTPTGTAVCTLGVAVNGRRKEGD
                     QWVDKPNYFNVVVWGAQGQNCANYLSKGRPVAVDGRLDWRSWEAKDGSGKRSTIEIVA
                     DTVQFLGSRDGSAPGGNNGGHSEANNGGNYGGQAQGGQGGGFTPSSDVPADPGDFAPT
                     SGGAPADDDIPF"
     gene            complement(8780..9175)
                     /gene="rpsF"
                     /locus_tag="PLJ64_04915"
     CDS             complement(8780..9175)
                     /gene="rpsF"
                     /locus_tag="PLJ64_04915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNC92863.1"
                     /translation="MTDSSRQYELVLMLDPQVEEAAREKLVSETKSAIEANGSLKHED
                     NWGIREMAYEIDRKSEADYRLFRFDGERELLGTLDHSLKIADGVLRFRIFRVDPDAPV
                     LPAPPATAVVMAPTGDDDERRGRGRYRDE"
     gene            complement(9831..11261)
                     /locus_tag="PLJ64_04920"
     CDS             complement(9831..11261)
                     /locus_tag="PLJ64_04920"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNC92864.1"
                     /translation="MATVRKRDFRTKILIALPVLLLALLVASGSAAGNAGARPKLRKC
                     DIGANRPMLCGHIMVPAVRGKPEAGRQKIAFVVRSRGDRSRPSLGAFVYIEGGPGYAP
                     SSPDSFKPLAAVMAPFLKRRELIAIDQRGTGRSNALFCKGLQRGTVSYQRGLKQCARQ
                     LGVRREGYTTANSAADIEAVRKALGLPKRKMILYGDSYGTYLGQSYAARYGKGMKGLI
                     LSSAYPGNDPFWRTLYPAAQRAVTRSCNRAPNCSGNALGRLNRMLRRAGTRSKLASNV
                     LNYLVGDASSYAPGGYNHLNRVITEYLRGRGRELRQLVHPYGPDQGPADYFSVGMYAA
                     VVCNDYPVPWKRSSPIPARRRQLNRAIADFRPSNWWAPIRKKTWAYDSASDISACLSW
                     PKRSAGYQPPIPRGRKMPGRLDTLVLAGEFDSITSVREARKVNSRFPRGRLYIVPDRG
                     HASELYFPFTSPATPRIRHFVRALDR"
     gene            complement(11261..12736)
                     /locus_tag="PLJ64_04925"
     CDS             complement(11261..12736)
                     /locus_tag="PLJ64_04925"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HNC92865.1"
                     /translation="MDTDEQPLIIVSNRGPAQFDLGPEGERVLSRGGGGLVTALSGLV
                     KDRHALWIASAMTSEDVVVTEENDGRPIDVNLNGVDYRVLMVESDPQAYDRFYNVIAN
                     PILWFIQHYLWDLSNAPDIRREEITAWYDGYEVVNEDIARCVIEQIADREDPLVMLHD
                     YHLYTAPAKIRSARPDVLLHHFVHIPWTQPDSWRVLPTPIREAIFNGLLANDIIGFHT
                     QSYCSNFLSCCRRLMGYEVDYEENVVTHPGGRTMVRAYPLSIDSDRLERAVHSERVAE
                     LEKEILARRREHLILRVDRADLSKNVLRGFTAFDTFLTDHPKFREKVTFVAHLQPSRQ
                     DVPEYAEYLERIEALVAVVNHRHGTTDWMPIDLRIYDDFDEAVARYKHCDLLMVNAIF
                     DGMNLVAKEAPVINVRDGVLMLSENTGSHEELKEFALTVNPFDIQDQADVMYRALTMS
                     PEERRERAEGLKRIIHSRTPADWIDEQLADIKLARTERESL"
     gene            complement(12802..>14042)
                     /locus_tag="PLJ64_04930"
     CDS             complement(12802..>14042)
                     /locus_tag="PLJ64_04930"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNC92866.1"
                     /translation="RPLPRVGSPFPVGSGAMPKRFSILVLVALLGLAVLAGCGSDSGA
                     DSNGDPGSDQDAKPIATSDADPVLLDSNGSEFNPSKVYAETIDGVVSIRSIFGDIENS
                     PATTSVAGGSGFVLADNGEIVTNAHVISDGNGDDRKAADEVYVEFSSGDVLDAEIVGF
                     DPFADVGLLKVSNDQVEMKPLTLADSDKVAVGQPIAVIGSPFGEDQTLTTGVVSQTGR
                     SVMSLTDFQIEGAIQTDASINPGNSGGPMLDGNGHVIGISQQMKSGSGSSDGVGFGVP
                     ANTIKRSVEMLRDGGEPQYAYIGVSTEPLYPQLAEKLGLDTDKGAIVAEVVEGGPADE
                     AGIRGSDQRLIFQGARYDVGGDVIVGVNGVSIERAEDLGRLVGALKPGDTARIEVIRD
                     GEHKEIDVELENRPTAVTRP"
BASE COUNT         2303 a         4514 c         4636 g         2589 t
ORIGIN      
        1 cgggaaaccc tcgcccggga aacgatgtcg gcgacactga cctcggcgta gcgcttctcg
       61 gcgcaggcct gggccatcga gtcgagcagc cggtcccgct gcgaggtgac gacgaactcg
      121 cggggaaggt tgtgccggcc ggagggaagc cgggaggggg tgatttcgct catgcggaca
      181 ccctggtttc ttccatcgcc tcgatcccgc ggtcatggcc gaaatagggg gtgaccgcga
      241 ggtacatcat gtccggcagc attttcggca ggtcggcggc gcggccgttc gatgcctcgt
      301 tgaacatcag cgcggaaacc ccgccgacga tcaccctcgc gacctgggtc gggatgcgat
      361 ccggttcgtc caccatgttg cgggccgaat ccagaaacgg gaaaaaggac gagacggcct
      421 gctgatagcg gtccctggcc cggggccccg ccccgaagcc ttcgacgatt ccgacccgcg
      481 ccgcgaccgg gttctcggcc aggtaggtga gcagggcgcc gattccgccg cggatctgga
      541 cgacccagtc atcggagctg ccctgggcaa acccgtcggc gacgaaggtg agcatccgtt
      601 ccatcaccgt tccgtaggcg gcgatgaagc aatcctcctt gtcggcgaac tgctcgtaga
      661 aggttgcccg ggagacgccg gcagtggaaa tgatgtcggc cacggtggct tcggtgtagc
      721 ccttttcggc aactacgccg atcatcgctt cgaccatgcg ttcacgctgg gagcggacca
      781 ccacatcgcg cggcagtccg tgccggccgc tcggaagctt gcccgaaccg aacgggtctt
      841 gttcttcctg tacctgctcc tgcggcattg gccgatctta ccccgcttga gtggactgtg
      901 aaacggaacc gtacctgaaa ggtctgtcgc ggtccggata tcggaccgga atggggagat
      961 ttcagtatct aaactcccct ctgtgaacga tcttccttca accggcggac ctgtcgggat
     1021 cgtcggcctg ggatacgtcg gcctgccgat cgccgtcgcc tttgcggagg ccggccggaa
     1081 agtgatcggc ctcgacaacg acccggccaa gatcgagaag atcaatcggg cggagagcta
     1141 catcgaggac attccctcgg caactctcgc cgctctggag gatcgtctgg tcgcgaccgc
     1201 gagctacgag gatctggccg gttgcgaagc ggtgatcatc tgcgtgccga ccccgctgac
     1261 cggttcgcga gaaccggacc tcagctacct ggagtcggcg gccgagagtc tggccggtgt
     1321 gctggcaccc ggccagaccg tgatcctcga atcaaccacc tgcccgggaa cgacccgtga
     1381 cttgctggcg aggacactcg aatcgggcgg tggtaagtcg ggggaggatt ttcacctcgc
     1441 cttttccccg gaacggatcg atccgggccg caccgacttc acggtccgca ccaccccgaa
     1501 actggtcggt ggtctcaccc ccgcctgtac cgacaaggcg gtggcgcttt acgaaacgat
     1561 ctgcgaccgc gtgatcccgc tctcgggccc tgaggctgcg gagctttcga agctgctgga
     1621 gaacatcttc cgctcggtca acatcgcgct ggtcaacgaa ctggcacagc tctgcgaccg
     1681 gctcggcgta gacgtctggg aggtgatcga tgccgcttcg accaagccct tcggtttcat
     1741 gcggttcgag ccgggccccg gcatgggcgg tcactgcctg ccggttgatc ccttctatct
     1801 cgccttcaag gcacgcgagc atgacttcta cccggagttc atcgagctcg ccggcaagat
     1861 caaccagtca cagccgctct tttgcgtcaa ccggatcgag cgggttctca atgcgaacgg
     1921 caagccggtc aaaggcacca aggtcctgat cctcggcgtc agttacaagg ccggggtcgg
     1981 cgacacgcgc gagtcgcccg cgatcaagat catccggctg ctgggtgagc tgggcgcgga
     2041 cgtgcgctac cacgacccgc atgtgccggc cttgaaggag gccggactga ccaatacccc
     2101 cgacctcgac ggcgccctcg ccgacgccga cctcagcgtg atcatcaccg cccacccgga
     2161 attcgactgg gcggaggtaa tcggaaagag tgacctggtc tttgacctgc gcggggtcac
     2221 ccgggagttc gatgatcccg gcaacgtgac gaggctctga ccgggccggg aagaaggagc
     2281 gcgggcttga ttcgggaact aaagggcgcc gcgtcgagcc tgatcccctg gcgacgggcg
     2341 cctcactctc actggcagac gatcaagagc gaccgggcga ccctcgccct ggccgtgggc
     2401 acggtgacga tcgccggact ggttctcgcc gcgcagtact cgcggctgct ttcccgccgc
     2461 acccattcac ccgggaccga gggtctgatc gattcggccc cggcggccgc ggtcgacacg
     2521 gtcggggtcg cggtcgaggg gtactcggcc accccgaacc gggagctgat cctcttcaac
     2581 ctgctgaccg gttttctcgg cagcttctcc gcggtccgga tgaccacctg ggcgatccgg
     2641 gaaggctggg gccctttccg caacgtcagc atcggcggcc gccacattca ccatttcgtg
     2701 ccggggatcc tgatcgggtt cggctccggc atcgctgccc tgctcctgaa cggcgagaag
     2761 gccgaccggc gaatcgcccg cagcctcggg gtcgggatgg gcctcacctt tgacgaggcg
     2821 gccctgctgc tcgacatgca ggacgtgtac tggaccaggc aggggctgct ttcggtacag
     2881 gtgaccctgg ccaccggggc taccctcggg gcgacgatcc tgaccatgcg gatcctcggc
     2941 cgcggcgaaa cccgccagga ggaggcgggt gagatcccgg cccccgaggg acagatgaac
     3001 acggttgtac cgtggcctca cccggctatg tagacagcca cgtaccgaga cgcaacgttt
     3061 ttgtaatttg tccgcccggc ccgctccctg gagcgcaatt cccttgttca agggggattc
     3121 gaggcataaa aaatgtcggc ggtcgggctg acccaaggga gagtgggcca acccgacttc
     3181 accgactaag cgctgctgag ggtagggagg gaccccagtc aacgatgttt tgcagttatc
     3241 acgtccagtg atctaactgc tgatacgaca tagtacacca aacacacgcg tttatgtgtc
     3301 ccgcggcaaa aagtctttca ggggcgtttc cgaaacctgt cccacccccc gctcgaacca
     3361 gcaccacccg agcccccgaa ccagcccgac ctgagtcccc gaaccagcac cacctgagcc
     3421 cctcccaacc atcagttact tcaagtggga ggggcgggtt gatcccggag ggcgggcaaa
     3481 gacccgcccg actgcggtca gtccgtgccg gcccgactgc ttatgactgg atccagacca
     3541 cctcgtcgcg ggccggtccg accccgacca ggttgatcgg caccccgacg tagtcgctga
     3601 tgaagctgat gtattcccgg gcggccggcg gcagatcctc ctcggtccgg cagccggtga
     3661 tgtcctcgct gaatccgggc aggtcaacgt actccggagt cgcgttgtgg aggatgctct
     3721 ggtggtaggg aaagtcctcg agcagcgcac cttccttcga gcggtagcgc accgcgaccc
     3781 tgatcgtgtc caggccggtc aggacgtcga gcttggtcat cgccaggtgt gaaagccggt
     3841 tcagccgcac cgcgtacttg agggcgacca ggtcaagcca gccgcagcgg cgacgccggc
     3901 cggtagtggt gccgaattca tgccccttgt ccgacatgta ctcgccgact tcgtcgaaga
     3961 gttcggtcgg gaagggcccc gaaccgaccc gggtcgagta ggccttgacg atgccccaga
     4021 cctcttcgat gtcggtcggt ccgaccccgg ccccgacgca ggcggctccg gcgatcggat
     4081 tcgaggaagt gacgaaggga taggtgccgt gatcgaggtc gagcagggcc gcctgggcgc
     4141 cctcgaagat caccttgttg ccggcgtcga gctcgttcca gcagagccgg gcggtgtcgg
     4201 cgatgtaggg ctccagccgg tggccgtagc tcaggtagtg ctcggtgatc gtgtgcaggt
     4261 cgagccggtc atcgatctgg atctcctcgt ccggctcggc ctccttgtgc aacttgcgcc
     4321 gcctgaccgc cagctcctgg agcgacttct tcttgtgctc gagggcggcc atgatcttct
     4381 tgcggaggat cttctcgtcg agcaggtcct ggacccggat cccgatccgg taggccttgt
     4441 cggcgtaggc cgggccgatc ccgcggcggg tggtgccgat cgagagcttg cccagcttga
     4501 gttcctcggc cgcatcgacc agcacgtggt agggcatgat caggtgggcg ttcgatgaaa
     4561 cccgcaggtt cgaggcggag acgccggccc gatgcagccc ctcgatttcc tccagcagaa
     4621 cccggggatc gacgaccacg ccgttgccga tcacgcagta cttgtcctcg tgcaggatgc
     4681 cggaagggat caggtgaaac ttgtactcct cgccgtcgtg cacgattgtg tggccggcgt
     4741 tgttgccgcc ctggaagcgc acgatcgcgg aggcgtcctt ggccagcagg tcggtgacct
     4801 tgcccttgcc ttcatcgccc cactgggttc cgactattac gagtcctggc atgaagaggg
     4861 cagtctagct tcagccgtcc cggagttgcg gcttctgccg ggcgtcctcg ccgaatagcg
     4921 ggaccacgct ttcaccgcac atctcggtca gccgcgaaac cgttcggggg ccgatctgct
     4981 gctcgagttc gccctggtcg gtgatgttgg tggtgaccac gatcgacttc tcgcgctcgt
     5041 agcgttcgtt gaccagggcg tagagctgtt cgagcaccca ttcggtctgc ttttcggcgc
     5101 cgaggtcatc gatgtggagc aggtcgacct cgcagagtcg ctcgaagaac tggtcgtagg
     5161 actcttcgcc ggcctccgcc ccgtaggtcg cccgaatccg ggtaagcagg ctgggcatcg
     5221 agtagatcgc gaccgtgtga tcccgcctga gcgcttcctt cgagaccagc atggcgaggg
     5281 tggtcttgcc tgtgccggtg tcccccatca gccagagtcc gttgccctgg tcaaggttct
     5341 cgtcgagccg ttcgatccag tcccggacga cggttaccgc accggggtgg atctcggtca
     5401 ccggcggacg gtcgaaggaa acgccgcggt agcgcttcgg caggaccgag ttgactccgg
     5461 aagcgcgggc cttcgccagc cggggctgcc ggcacctgca ttcggttgcc gagccgtcct
     5521 cgttcagaat ccagccgttg ccgtcacagg cgccataggg gcagggcggc tggccgttgc
     5581 cggatgcctc gaattcgccg gccgccatca gatcgcgtcc gcttccgcct ggaccgcctg
     5641 cggcgagccc tcgccggcca tgtgctcacg gctttcctcg gcgaatttcg ggatgtgcgg
     5701 gaagaagatc aggtggctgg tgccgatcgg gccgctccgg ttcttggcga cgatcacctc
     5761 cgcctttccg gtgtgctttt cccggtcctt ctggtagacg tcttcgcggt agatgaagag
     5821 gaccaggtcg gcgtcctgtt cgatgttgcc cgactcgcgc aggtcggaga gcatcggccg
     5881 tttgtcggtc cggctttcgt tcgcccggtt gagctgcgaa agcgcgacga ccggcacttt
     5941 cagttcgctg gcgagcagct tgaggccacg gctgacctgc ccgacctgct cggcccggtt
     6001 ggcaatccgg tcatccatcc gcatcagctg caggtagtcg acgatgatca gtgaaaggcc
     6061 ttcgttttca cggtccgtgc ccgcacgccg gcggatctcc tgctcatgca gtcgccgcgc
     6121 cttggcccgc agatcgagca gccccaggtc cgaggtgacg tcaacgaaca ggggcacgtt
     6181 ggccagggcg ttcagggact tgttgatctt cttccaggtc cggtcgcccc ccttgaatct
     6241 ggctttccgg aggatgtcgc cgtgaattcc ggaatgggag gcgatgaacc tctgggagag
     6301 ttccccttcg gacatttcga gcgagaagaa cgccaccgac ttgtcctggc ccaccgccac
     6361 gttttcggcg atgttggcga cgaaggctga cttgccgacc gagggccggg ccgcgaggac
     6421 gatcaggttc gaaggctgaa agcccgccgt cttctcgtcc aggtccggga accccgtgtt
     6481 ggttcccacc tccttcagct cgccgctgat ccgtttctcg agccgctgat gctcctcgaa
     6541 aagcatgttc tcgaggctcc ggaaatcgcc ctcctggtcc ctgtgggcga ccttgaagag
     6601 ttcggcttcc gcccgttcgg aaagcgtgcc ggcgtcagct tcgcggtcgt tgacccagac
     6661 ctcgatgtcc tgggccgccc gcagcaaccg tcgcaggtgg gcatgctcgc ggacgatctc
     6721 ggcgtggtgg cgggcattcg ccggggcggc gaccttctcg cccagttccg ccacgtagtt
     6781 ctcgccaccg gcctcgtcca gcctgccgct ggtgaccagg gcttcggtca cggtcagggt
     6841 gtcgcaggcc ttgtcggccg catacaggtc gatcatcgcc tcgtagatcg cctgatggcg
     6901 gtcgaggtag aaatcgcccg gcttcagttt gacttcatct acggcccagt tgacggcagg
     6961 ctcgtagacc agcatcgagc cgaggactgc ctcctcggct tcgacgttgt tgggtggaac
     7021 ctgggctatt tctcttccgc gacgatcgtc ttgaccgagg cgatggcgcc accggccact
     7081 tctacgtcaa tcatgtaggt gccgacctgg tggatcggca cgtcgagacg gatcttcttg
     7141 cggtcgacct tgatgccacg ggcatcgcgg atcgcctcgg cgatctcctt ggtcgtgacc
     7201 gagccgtaaa gcttgccgtc atcaccggca cgatggttga tcgtaagcac ggtcttggaa
     7261 agcagggaag cgttctcggc ggcgcgggcg atcgcctcgc gctcggcctt ctcggcggct
     7321 tccttgcggc ggatcgcttc ctcgagggcg cccggggtgg cgggctgcgc cagaccgcgg
     7381 ggctgcaggt aattccggag gtagccgggg gaaacctcga cggcggtgcc gtgctctccc
     7441 agcccctcga cgtcagcgag cagaatggcc tgggccatta cttctcaccc acgtagggga
     7501 gcagggccac ttcgcgggcg cgctttaccg cgaccgacag ctgggcctgg tgcttgcggg
     7561 aaaggccggt gacccggcgg gagcgggtct tgcccttgtc cgagatgaac cgacgcagca
     7621 gggtcagatc cttgtagtcg atgttctcgg cggaaatctt cgtgtacttg cgggggcgta
     7681 cgcgctcgct aaagcggcgc ccacggcgcg atgacgcttc tcgtgtctta gccaatgctt
     7741 tccggtgcct tacatagttc aagcccgggc aagttcccgg gcattcagga caaggaatcg
     7801 taccggttcc cgggcctgtg cgacttctgt accaacccca tcggtcgcaa actccgcaaa
     7861 acatggttga gcgaggtggt tacgccgcag ggtcgggtga ggatcgccgg gacggacgga
     7921 cgcgcggcat tcagccgcgc tggtcctgga gtgtcccgga cgacccccac ccgaccccac
     7981 ggctacacca acgggagtca agggacgagg gtgacgcagg tttgcaacct ctgcggttgc
     8041 aaacctgcgg caccccgaga aggcccgcac ccggtcaggc ggtcggttag aaggggatgt
     8101 cgtcgtcggc cggggctccg ccgctggtgg gggcgaagtc tccggggtcg gcgggaacgt
     8161 ccgaggaggg ggtgaaacct ccgccctggc caccctgggc ctggccgccg tagttgccgc
     8221 cattgttggc ctcgctgtgc ccgccgttgt tgccgccggg ggccgagccg tcccgggaac
     8281 cgaggaactg gaccgtgtcg gcgacgattt cgatcgtcga ccgtttcccg gaaccgtcct
     8341 tggcctccca ggagcgccag tcgagccggc cgtcaaccgc gacgggacga cccttcgaaa
     8401 gatagttggc gcagttctgc ccctgtgcgc cccagaccac cacgttgaag tagttgggct
     8461 tgtcaaccca ctgatcgccc tccttgcggc ggccatttac ggccactccg agagtgcata
     8521 cggcagtacc ggtcggcgtc tgccgcagtt ccggatccct ggtgaggttg ccggtgatcg
     8581 tgaccctgtt gatgttctcg gccattgtgt gtttgctcct tatcgcgttt ttcgtacatc
     8641 gcggatcccc tgtcgaaccc gcgggtcgga ggaggaatgt agcgacgcct gtggacgctt
     8701 aaaggcaaag ggggcgaatt ctcgcccccc gattacgcaa ataccgcgga aagattcacg
     8761 gcaccacggc ctggatgcct tactcgtcgc ggtagcgacc gcgaccgcgg cgctcgtcat
     8821 cgtcaccggt cggggccatc accacggcgg tggcgggcgg ggccggcagg accggagcgt
     8881 ccgggtcgac ccggaagatg cggaagcgca ggacgccgtc ggcgatcttc aggctgtgat
     8941 cgagcgtgcc gagcagctcg cgctccccgt cgaagcggaa cagccggtaa tcggcttcac
     9001 tcttgcggtc gatttcatag gccatctcgc ggatgcccca gttgtcttcg tgcttgagcg
     9061 aaccgttggc ttcaatggcc gacttggttt cggaaaccag cttctcacgg gctgcttcct
     9121 caacctgggg gtcgagcatc aggacaagtt cgtattggcg gcttgagtca gtcaaaatcg
     9181 acgggggact ataccttcat cgcccgattt ccgcggtgcg atgtggatta caacccggtt
     9241 ccggtccctc cagggggaga gtctgcccgc ccgggccctt gatcaggtat gcgaagtggc
     9301 atccggagcc tcagggttga ccgcgagcgc gcgatcaggc tgggcgcgat catcggcctc
     9361 gtcctgctcg gtgcctcgac cctgcccggt ctgctgaaaa ccccggaccc tccgccggtt
     9421 ccggccaacg tgggattccg cccggccgag atgaacgggt tcgcagcgca acccggtccc
     9481 ggggaggcga tccgcaagga agctgcgaag aagaagcggg agcggacgct gaaacggaag
     9541 cagctcgaag ccgggcgaaa gaggcgagcg cggaaaagag aagagtcccg gaggcggcgg
     9601 gcatcgaagc ggaacagaaa acaccaccgg ggcggggatg aaccggccgc cggtccggct
     9661 tcggactcgg ggccgacttc cacgatcagc ccttctccgg ctcccgcggc cagcccttct
     9721 ccggctccgg tctactcgcc tccggcctcc gtgccaccgc ctcctccccc gtcaccggct
     9781 cccgcgccgc agcccgccga cgggtcgcag gaatttgcgc cgcgttgaag ctagcggtcg
     9841 agggcgcgga cgaagtggcg gatccggggc gtcgccgggg aggtgaaggg gaagtagagc
     9901 tcggaggcgt gaccgcggtc gggcacgatg tagaggcggc cgcggggaaa ccgcgagttg
     9961 accttccggg cttcccggac cgaggtgatc gagtcgaact cgccggccag aacgagcgtg
    10021 tccagacggc cgggcatctt ccggccgcgg ggaatcggcg gctggtaacc ggcggaacgc
    10081 ttcggccagg aaaggcaggc gctgatgtcg ctcgcggagt cataggccca ggtcttcttg
    10141 cggatcggag cccaccagtt cgacggccgg aagtcggcga tcgcccggtt caactgccgg
    10201 cgccgggccg ggatcgggga tgaacgcttc cacggcacgg ggtagtcgtt acagaccacg
    10261 gccgcgtaca tcccgaccga gaagtaatcg gccggcccct ggtccgggcc gtatgggtgg
    10321 acgagctggc gcagctcccg gccccggccg cgcaggtatt cggtgatcac ccggttgagg
    10381 tggttgtaac cgcccggcgc gtagctcgag gcatcaccga ccaggtagtt caggacattt
    10441 gaagcgagtt ttgacctggt cccggcccgg cgcagcatgc ggttgagccg gcccagtgcg
    10501 ttgccggagc agttcggcgc ccgattgcag gaacgggtca ccgcgcgctg cgcggccggg
    10561 tagagggtgc gccagaacgg gtcgttgccg ggataggcgg aggaaaggat caggcccttc
    10621 atgcccttgc cgtaccgggc tgcgtaggac tggccaaggt aggtgccgta ggagtcgccg
    10681 tagagaatca tcttccgctt cggcagaccc agcgcctttc tcaccgcttc gatgtccgcc
    10741 gccgagttgg cggtcgtgta cccctctcgc cgcacgccca gttgcctggc gcattgcttc
    10801 aacccgcgct gatagctgac cgtgccgcgc tgaaggccct tgcagaagag ggcattcgag
    10861 cggcccgtgc cgcgctggtc gatcgcgatc agttcgcgcc gcttcagaaa cggggccatc
    10921 accgcggcca gcggcttgaa cgaatcgggg ctgctcgggg cgtagccggg cccgccttcg
    10981 atgtagacga acgcacccag cgagggccgg ctccggtccc cccggctgcg gaccacgaag
    11041 gcaatcttct gccggcctgc ttcaggtttg ccccgcaccg ccggcaccat gatgtgaccg
    11101 caaagcatcg gccggttggc gccgatgtcg catttcctca gcttcggcct cgcccctgcg
    11161 ttcccggccg cggagcccga ggcgacgagg agcgcgagca ggagaactgg cagggcaatg
    11221 aggatcttcg tccggaaatc gcgcttccgg acggttgcca tcagaggctt tcccgttcgg
    11281 ttcgggccag tttgatgtcc gcgagctgct cgtcgatcca gtcggccggg gtccgtgaat
    11341 ggatgatccg tttcaggcct tcggcccgtt cgcgcctttc ctccggtgac atagtcagcg
    11401 cccggtacat gacatcggcc tggtcctgga tgtcgaaagg attgaccgtc agggcgaact
    11461 ccttcagctc ctcatgcgag ccggtgttct cggacagcat caacacgccg tctctcacgt
    11521 tgattaccgg agcttccttg gcaaccagat tcatgccgtc gaaaatcgcg ttgaccatca
    11581 gcaggtcgca atgcttgtag cgggcgacgg cctcgtcgaa gtcgtcgtag atccgcaggt
    11641 cgatcggcat ccagtcggtg gttccgtgac ggtgattgac gaccgcgacc agagcctcga
    11701 tccgttcgag gtactccgcg tattcgggca cgtcctgcct tgacggctgg agatgggcga
    11761 cgaaggtgac cttctcccgg aacttggggt ggtcggtcag gaaggtgtcg aaggcggtga
    11821 agccgcgcag cacgttcttc gaaagatcgg cccggtcaac ccgcaggatc aggtgttccc
    11881 gccggcgggc aaggatttcc ttttcgagct cggcgacccg ttcggagtgg accgcgcgct
    11941 cgagccggtc ggaatcgatc gaaagcggat aggcgcgaac catcgtccgg ccgccgggat
    12001 gggtcaccac gttctcctcg tagtcgacct cgtagcccat cagccgccgg cagcaggaaa
    12061 gaaagttgga acagtagctc tgggtgtgaa agccgatgat gtcgttggcg agcaatccgt
    12121 tgaagatcgc ttcgcggatc ggggtcggca gcacccgcca ggagtcgggc tgggtccagg
    12181 ggatgtggac gaagtgatgg agcaggacgt cgggccgggc ggaccggatc ttggccggcg
    12241 ccgtgtacag gtggtaatcg tggagcatga cgagcgggtc ctcgcgatcg gcaatctgct
    12301 cgatcacgca cctggcgatg tcctcgttga ccacctcgta gccgtcgtac caggcggtga
    12361 tttcctcacg ccggatgtcc ggcgcgttgg agaggtccca aaggtaatgc tggatgaacc
    12421 agagaatcgg gttggcgatc acgttgtaga agcggtcgta ggcctgcgga tcgctttcga
    12481 ccatcagcac ccggtaatcg accccgttga ggttcacgtc aatcggccgg ccgtcgttct
    12541 cctcggtcac caccacgtct tcgctggtca tcgcggaagc gatccacagc gcgtgacggt
    12601 ccttgaccag tccggaaagg gcggtaacca gtccgccacc gccccgggaa agaacccgct
    12661 cgccttccgg cccgagatcg aactgggcgg ggccccggtt cgagacgatg atcagtggct
    12721 gctcgtccgt gtccacggga accattctat ggcgtccggt tttccccgga cgacctgtga
    12781 aacccgtccg atcgcgggag gctaggggcg ggttactgcg gtggggcggt tttccagttc
    12841 gacgtcgatc tccttgtgct cgccgtcgcg gatcacttcg atccgggccg tgtcaccggg
    12901 ctttagagct ccgaccagcc ggccgagatc ctcggccctc tcgatcgaca cgccgttgac
    12961 accgacgatc acgtcgcccc cgacgtcata tcgcgcgccc tggaagatca gcctctggtc
    13021 actgcccctg attcccgcct cgtcggccgg gccgccttcg accacctcgg ccacgatcgc
    13081 ccccttgtcc gtgtccagcc ccagcttctc ggccagttgc gggtaaagcg gttcggtgct
    13141 gaccccgatg taggcgtact gcggttcacc gccgtcccgg agcatttcca ccgagcgctt
    13201 gatcgtgttc gccgggacgc cgaaaccgac cccgtcactg gatcccgagc cgctcttcat
    13261 ctgctggctg atcccgatca cgtgaccgtt gccgtcgagc atcggaccgc cggagttgcc
    13321 ggggttgatc gaggcgtcgg tctggattgc gccctcgatc tggaagtcgg taaggctcat
    13381 cacggaacgg ccggtctggg agacgacccc ggtggtcagg gtctggtcct ccccgaaggg
    13441 gctgccgatc accgcgatcg gctgaccgac cgccaccttg tcgctgtcgg caagggtcag
    13501 cggcttcatc tcgacttgat cgttggagac cttgagcagg ccgacgtcgg cgaaggggtc
    13561 gaagccgacg atttcagcgt caagcacgtc accgctggag aactctacgt agacctcgtc
    13621 ggcggccttg cggtcatcgc cgttgccgtc gctgatcacg tgggcgttgg tgacgatctc
    13681 gccgttgtcg gcgagcacga atccggagcc gccggcgacc gaggtggtgg ccggcgagtt
    13741 ctcgatgtcg ccgaagatcg aacggatcga gacgaccccg tcgatcgtct cggcgtaaac
    13801 cttcgacggg ttgaactcgg aaccattcga gtcgagcagg accggatcgg cgtcggaggt
    13861 cgcgatcggc ttcgcgtcct gatcgcttcc cgggtctccg tttgaatcgg caccggaatc
    13921 cgagccacag ccggcaagca cggccagccc gagcagggca accagaacca ggatcgaaaa
    13981 gcgcttcggc atggcgccgg agcctaccgg gaagggcgat ccgacccgcg gaagtggtcg
    14041 tg
//
LOCUS       DAOQKN010000049        13891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_2061_length_13891_cov_5.901923, whole genome shotgun sequence.
ACCESSION   DAOQKN010000049 DAOQKN010000000
VERSION     DAOQKN010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 13891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13891
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2061_length_13891_cov_5.901923"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(211..873)
                     /locus_tag="PLJ64_04935"
     CDS             complement(211..873)
                     /locus_tag="PLJ64_04935"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNC92867.1"
                     /translation="MALQLGQEAPNFTAETTEGTIDFHDWIGDSWAVLFSHPKDFTPV
                     CTTELGYMANAKPAFDERGVKIIGLSVDPLDSHEDWAKDIEETQGTAPNYPLISDADF
                     KIAKAYGMLAADVEGDAADRTPADNQTVRNVFVIGPDKKIKLILVYPMTTGRNFDEVL
                     RVIDSLQLTANHKVATPANWKSGDDVIIAGSVNNDQAKEIFGEWEEPKPYIRIVPDPT
                     AS"
     gene            complement(955..1446)
                     /locus_tag="PLJ64_04940"
     CDS             complement(955..1446)
                     /locus_tag="PLJ64_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92868.1"
                     /translation="MELKPKQSDKPGKERGNDFERVHGDLLRSFLEPGESLLGVAAVN
                     WQRSMFKQTVSALGVTGSRLIIQPLDRKGKAATEAPTFIRKEEITKGSYGGGGGMGNS
                     PTSLIMDSASIEVKLKTSGGEKYKLLLMTGEGMFGSMAGGPAQRNGAEALVSFLEGAG
                     RSI"
     gene            complement(1464..1844)
                     /locus_tag="PLJ64_04945"
     CDS             complement(1464..1844)
                     /locus_tag="PLJ64_04945"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNC92869.1"
                     /translation="MTEIIAPKVGKNSPRQAWLLDFNLAFVGGDIEQTLGFVAEDVTW
                     ELVGEGTIKGRDGMRAWLQSMAGKQARRVEFRLFITHGRSAAVNGSYEMESGSKFEFC
                     DIYEFANAKNDSPITNYTSYVVRV"
     gene            1926..2648
                     /locus_tag="PLJ64_04950"
     CDS             1926..2648
                     /locus_tag="PLJ64_04950"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HNC92870.1"
                     /translation="MIALILLASATALATGLGAIPVMLLGNRARSIVPLLLGIAGGVM
                     AVASVMGLIIPALDRGTVAEVVGGLAIGTGFLLLARRYLSHVPRNFSRSGGRTGLLVF
                     LVLLVHSLPEGFAVGTAYEADPAQLGIFVVIAIAIQNIPEGTSVAIPLAAAGASGRRQ
                     FWMAVASSVPQPIGAVIAYLLVQEISALLPVSFGFAAGAMLALTLLEILPEAWIENPR
                     QTFLGLATSIPLMVALSLALGV"
     gene            complement(2705..3106)
                     /locus_tag="PLJ64_04955"
     CDS             complement(2705..3106)
                     /locus_tag="PLJ64_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92871.1"
                     /translation="MTACGSDDSSSDDSSESITKAEFVAQANEICAESNKTIDAAEKE
                     AFSGGQPTQADVDSFINDTVLPQVESQINDIEALPVPEGEEDQISAILDAANKALEEG
                     KSDPSSLQGNGDPFAEANKLANAYGMTECGG"
     gene            3213..3926
                     /locus_tag="PLJ64_04960"
     CDS             3213..3926
                     /locus_tag="PLJ64_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92872.1"
                     /translation="MCDGSEMNRDDERIIEGMRRQSEGISKSGAGGAANLERLGWKAG
                     FGTGATMALIGTSQPLAGFLTKATLLDSGAFVQVGGWGVPLFEAEVAVRIDSEIGAGA
                     TAGEVTAAAGAVAAAIELVDFGSIESVEEILAGNIFHRHVLLGEFVELPPDGLDGVSI
                     SVAANGEAAEASDPREALGDLGQVVAALADQAALTGESIKPGDVVITGAAIPPAPVSP
                     GDDYGVEITGGSSVTVKLV"
     gene            complement(4021..4094)
                     /locus_tag="PLJ64_04965"
     tRNA            complement(4021..4094)
                     /locus_tag="PLJ64_04965"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4058..4060),aa:Pro,seq:ggg)
     gene            complement(4167..4237)
                     /locus_tag="PLJ64_04970"
     tRNA            complement(4167..4237)
                     /locus_tag="PLJ64_04970"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4203..4205),aa:Gly,seq:tcc)
     gene            complement(4308..4380)
                     /locus_tag="PLJ64_04975"
     tRNA            complement(4308..4380)
                     /locus_tag="PLJ64_04975"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4345..4347),aa:Trp,seq:cca)
     gene            5023..5238
                     /locus_tag="PLJ64_04980"
     CDS             5023..5238
                     /locus_tag="PLJ64_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92873.1"
                     /translation="MNTFKMALKDGPKEYSYPRYFFTNYSADIQAIFCRTCDRLGIRW
                     SRSNWRNISISHRTSVALLDEFVGPKD"
     gene            5260..5877
                     /locus_tag="PLJ64_04985"
     CDS             5260..5877
                     /locus_tag="PLJ64_04985"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HNC92874.1"
                     /translation="MSDRLKIVSVLGSVTAPGRMHRALNEALDRAGTGAGGATGDGEV
                     AVERIDLAEKTVTFADGTPLDQLGDDSAAVIGQIARADAVVLATPVYRGSMTGALKNL
                     LDLTPVEALRGKPVLIVAMGATDHHSLGVERHLRDVLSFFGALPVPVDVYLTSADFTD
                     GVPGDDAARRLDQGLDGLLALTRATGGLGEFGPDPLITRLSRPQS"
     gene            complement(5955..7259)
                     /locus_tag="PLJ64_04990"
     CDS             complement(5955..7259)
                     /locus_tag="PLJ64_04990"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HNC92875.1"
                     /translation="MTDRPNIIYVHSHDTGRYVQPYGHHVLTPNMQLLADQGITFRRA
                     FCAAPTCSGSRAALLTGQHCHNNGMMGLAHRGWKLNDYSQTMVHPLKEGGYRTILVGE
                     QHISRDPHVIGYDEVIELSDNYAKRVAPSALEAIRRTSEPFFMSVGFFETHRDFAAPT
                     SVRDSLYSLPPENLPDTAETRSDMASFKESVRSLDQGIGALLNGLHATGLADRTLVIC
                     TTDHGLAFPKAKATLLDGGIGVMMLMRGPNGFSGGKVIDSMVSHLDIYPTVCELAGVP
                     PPDFLQGRSLMPLVSGESAALHEELFSEVTYHAAYEPQRAVRTNRWKYIRRFDDRKRP
                     VLANCDDSLTKDLLIAMGWGDREVAREQLFDLYLDPFEENNLAGDPGSADILVDMRSR
                     LDRWMVETDDPIRLGPIPPPPGAGINDPDQVSPDEPLTLIPG"
     gene            complement(7279..8187)
                     /locus_tag="PLJ64_04995"
     CDS             complement(7279..8187)
                     /locus_tag="PLJ64_04995"
                     /inference="COORDINATES: protein motif:HMM:NF020604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stf0 family sulfotransferase"
                     /protein_id="HNC92876.1"
                     /translation="MSKPERSYLVCATPRSGSTLVCQTLIRTGVAGRPEEYFEALRSS
                     GVPRQPEEYFHGVGDRTIFDHLAERAQDFNPPPRSPLWSRTAYDRYLDWVFDVGTTDN
                     GVFGAKLMWGYVEDFVSLLRNIPEFREVPMAEILSEVFPELTFVRVVRANKIRQAVSL
                     WKAVQTATWRDETTASSSADAEDIDASYKGFVNDHRPALRFHYGAIKHLLDQLIQQEA
                     AWDAFFEHSRIKPVFILYENFASDIGPSTRNVLERLGIEAPDDLNLETRMRRQSDGIN
                     DDWARRYSDIRLGAEFDLVPTPSGRD"
     gene            8284..8967
                     /locus_tag="PLJ64_05000"
     CDS             8284..8967
                     /locus_tag="PLJ64_05000"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC92877.1"
                     /translation="MSNAATDAAPPVPESVLVGGVGLGAIPKQQRGIEVRDRLYEAAV
                     AEIDAKGIEESRVENIVAEAGTSWGTFFRYFPRKEDIFLHASVRNFREYLKPAFDEVV
                     ADDSASIKEGARSIILLVMEPRVSPRFHAEMLAEVLRHPARFAAILGEGELPLAVLMA
                     GLLVEGQKRGEVTAEAPVPVCAAVLLAGVMFSASTVLQAVAEGTRPGSDIAEVANQAF
                     DLAWSGIGA"
     gene            9018..9101
                     /locus_tag="PLJ64_05005"
     tRNA            9018..9101
                     /locus_tag="PLJ64_05005"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9052..9054,aa:Leu,seq:tag)
     gene            complement(9145..9753)
                     /locus_tag="PLJ64_05010"
     CDS             complement(9145..9753)
                     /locus_tag="PLJ64_05010"
                     /inference="COORDINATES: protein motif:HMM:NF037271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC92878.1"
                     /translation="MGRSPSPYREATRELLRETVFRAARGKLEVRPWSEITMADIAVE
                     AGVSRQTLYNEFGSRNEFGLTYALHEAGKFLDGVEEAIRAKSDDPRAAVEVAFERFLL
                     IASGDPVIATLLSDDGTGGMLQFVTTRGTPMISWLAERIGVIIVETWPGYSADDANLI
                     SENLVRLAISHVTAPTGSRDETAARVGRLIGPFIDESFGTDS"
     gene            complement(9780..9944)
                     /locus_tag="PLJ64_05015"
     CDS             complement(9780..9944)
                     /locus_tag="PLJ64_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HNC92879.1"
                     /translation="MSRWVCPDCGYVYDEEKGHPREGFPPGTAWEAVPDSWACPDCGV
                     RDKVDFEPDD"
     gene            complement(9941..11179)
                     /locus_tag="PLJ64_05020"
     CDS             complement(9941..11179)
                     /locus_tag="PLJ64_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
                     /protein_id="HNC92880.1"
                     /translation="MDELTDKQPAAGPVAEEAPGHHHHHHHDEVDLNPAGWRDGKRYA
                     WLLGILVPLLPFISWQLVEATGLGIFWYSAPLVVFGLFPLLDTVIGLDPTNPPDSVLK
                     WLEQDRYYRWCTYMFIPVQYAGLVFACWQWADGGLSVFESIGLTLTMGVVGGIAINTA
                     HELGHKREAMERWLGRVALAQTGYGHFFIEHNRGHHVRVATPEDPASSRMGESFWAFL
                     PRTVSGSLRSAWGIEAARLDRLGVSHWSYKNDILSAWAMTVLLFGGLAVAFGPVVLPY
                     LIIQAVVGFSLLEVVNYLEHYGLLRQRKDDGRYERCQQQHSWNSNNVASNVLLFHLQR
                     HSDHHTHPTRRYQALRHAEEAPQLPTGYAGMIVLALFPPVWRRVMDPRLLAHYDGDVS
                     RINMQPRARRRIEARYGSAS"
     gene            11269..11802
                     /locus_tag="PLJ64_05025"
     CDS             11269..11802
                     /locus_tag="PLJ64_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92881.1"
                     /translation="MAVGEARPGYELWRPNREKAECLTTRFIAVVLLVASAALMLIVT
                     LAGWDVLVGGQTYGIISLIFVGVYILLAVMIWRWSRGALTLTMALSVLLGIFCAVGAP
                     TWFARDKAGFTEPGLPSDLIGLLVLLLIPVQIAVLLTCAIGFRQEWHVEEERPISSGD
                     ADAGGDQVESGSLAGAS"
     gene            11831..11914
                     /locus_tag="PLJ64_05030"
     tRNA            11831..11914
                     /locus_tag="PLJ64_05030"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11865..11867,aa:Leu,seq:caa)
     gene            11927..13036
                     /locus_tag="PLJ64_05035"
     CDS             11927..13036
                     /locus_tag="PLJ64_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92882.1"
                     /translation="MEAAEEKPTSPREKDEKTDALASGPTGVAGSDLERRAAARLVEE
                     LESKGRSARVQEMKIPASESATITLHAALTLAATLIGMKWPAIGATICLIAAFSFYSE
                     RGLGLRLIGRAIPGRRTGNVLSPPPGPAWEEVEVILATGYDVPDSYPVGEWLSRRFSG
                     RLTTDRILFYGGMIGTFAALMMRAVGIEDTALSIFQTLTAAIPLAAIAAQVDRHLAGT
                     PVATQEDLAPARDVMAAVREADDQSEGDSGVAVCLFGSEYSSAAGASAFFADSRLKLK
                     QGAALVNLVRGARASGPEVTGSEGDLMPTRMNPELAAGSPLKPKMVVLRNQTAATIAR
                     RRGLRATTVVGRGEAGVDVLLDASEGALPDEEEKE"
     gene            13033..>13891
                     /locus_tag="PLJ64_05040"
     CDS             13033..>13891
                     /locus_tag="PLJ64_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNC92883.1"
                     /translation="MSPEPLWSPDPEAIESSQLVAWAHWLKDRDLGPGTAEYADLWQW
                     STDQPEEFWRSIWDYFEVLHDGEPAAVLSGHEMPGARWFEGTSLNYAEHIFRDRDPEA
                     AAVFAASEVRALETVTWGELRSRVADCAAGLKALGVGPGDRVAGYLPNSHETIVAFLA
                     VASLGAVWSSCSPDFGAGAVTDRLAQIEPRVLLAVDGYNYGGKGFDRRGVVSDLVAGM
                     PTVEQVVILPYLEPDPDLTPLPGAITWNELLSKDPGAELTFTRVPFDSPLWILYSSGT
                     TGLPKPIVQS"
BASE COUNT         2307 a         4425 c         4624 g         2535 t
ORIGIN      
        1 ggccgagatg gagatcgtga tgaaggcggt ggcccgggcc gggatccttt tgccggtcgg
       61 cccccaggag cctgccgtgg cccgcttcat cacctcctcg ccgaagcgcg gcggccaggt
      121 ccgcttcgcc ttctagccga agcgggacga ggcttcgaga aggcggaagt cagccctatt
      181 gactgacttc cgccatctcg gtaaatccga tcagctggcg gtcgggtccg ggacgatccg
      241 gatgtacggc ttgggctctt cccactcgcc gaagatctcc ttcgcctggt cattgttgac
      301 cgagccggcg atgatcacgt cgtcgccgct cttccagttg gccggggtgg cgaccttgtg
      361 gttggcggtc agctgcagcg agtcgatcac gcggaggacc tcgtcgaagt tccggccggt
      421 cgtcatcggg tagaccagga tcagcttgat cttcttgtcc ggaccgatca cgaagacgtt
      481 gcggacggtc tggttgtcgg ccggggtgcg atcggcggcg tcaccctcga cgtcggcggc
      541 aagcattccg taggccttgg cgatcttgaa gtcggcgtcg gagatcagcg ggtagttcgg
      601 ggccgttccc tgggtctcct cgatgtcctt ggcccagtcc tcgtggctgt cgagcggatc
      661 gaccgagagc ccgatgatct tcacgccgcg ctcgtcgaag gccggcttgg cattcgccat
      721 gtaaccgagt tcggtggtgc agacgggggt gaagtccttc gggtgcgaaa agaggactgc
      781 ccaggagtcg ccgatccagt cgtggaaatc gatcgttccc tcggtggttt cggcagtgaa
      841 attcggtgct tcctgtccca gctgtagggc catgcttttc tccttcaaga attgactgtg
      901 aaccaactat agccaatatg tcccatatac ctgatccgga tacaggtatt taggtcagat
      961 cgagcgtccg gctccttcga ggaagctgac cagggcttcg gcgccgtttc tttgtgccgg
     1021 cccgccggcc atgctgccga acatgccttc gccggtcatc aggagcagct tgtacttctc
     1081 gccgccagag gtcttcagct tcacctcgat cgatgcggag tccatgatca gagaggtcgg
     1141 cgagttgccc atgccgccac ctcccccgta gctgcccttg gtgatctcct ccttgcggat
     1201 gaaagtcggg gcctccgtgg ccgccttgcc cttgcggtca agcggctgga tgatcagcct
     1261 gctcccggtc acccccagcg cgctgaccgt ctgcttgaac atgctgcgct gccagttgac
     1321 cgccgccacc ccgagcaggc tctcgcccgg ctcgaggaag gacctgagca gatccccgtg
     1381 gacccgctcg aagtcgtttc cccgctcctt tcccggcttg tcggactgct tcggtttgag
     1441 ttccatgcgc cgccccgctc ctttcagact cgcactacat acgaggtgta attcgtgatc
     1501 gggctgtcgt tcttcgcatt ggcgaattcg tagatgtcgc aaaactcgaa cttcgaaccg
     1561 ctttccatct cgtagctgcc gttgaccgcg gcagatcggc cgtgggtgat gaagagcctg
     1621 aactcgaccc gccgggcctg cttgccggcc atcgactgaa gccaggcccg cattccgtcg
     1681 cgacccttga tcgtcccctc gccgaccagc tcccaggtga cgtcctcggc cacgaagcca
     1741 agggtctgct cgatatcccc gccgacgaag gcgaggttga aatcaagcag ccaggcctga
     1801 cgcggcgagt tcttcccgac cttcggcgcg atgatctcgg tcatgcggcc agcctagtcg
     1861 ctgaaggagt gctcggcgaa gggccggtca ccgcctccgc catcgcccct acgctttgag
     1921 tcgtcttgat cgcactgatt ctcctcgcca gcgccaccgc cctcgccacc ggactcgggg
     1981 cgatcccggt gatgctgctc ggcaaccggg cccggtccat cgtgcccctg ctgctcggca
     2041 tcgccggagg ggtaatggcc gtggcttccg taatggggct gatcattccg gctctcgacc
     2101 gcggcaccgt agccgaggtc gtcgggggcc tggcgatcgg gaccggtttc ctgctgctgg
     2161 cccgccggta cctgagccac gtgccgagga acttcagccg gtccggcggg cgcaccgggc
     2221 tgctggtctt cctggtcctg ctggttcaca gcctgccgga ggggttcgcg gtcggcaccg
     2281 cctacgaggc cgaccccgcc cagctcggga tcttcgtggt gatcgcgatc gcgatccaaa
     2341 acattcccga gggaaccagc gtcgcgatcc cgcttgccgc cgcgggtgcc tcgggccgcc
     2401 gccagttctg gatggcggtc gcgagcagcg tgccgcagcc gatcggtgcg gtgatcgcct
     2461 acctgctggt ccaggagatt tccgccctgc tgccggtctc tttcggcttc gctgccgggg
     2521 cgatgctcgc cctgaccctg ctcgagatcc tccccgaggc ctggatcgaa aacccccgcc
     2581 agacctttct cggcctggcc acctcgatcc ctttgatggt cgccctgagc ctggcgctcg
     2641 gggtctgagc gaccggctgc gccttcgcct gacgaggccg gccgctctcg cacctatcgg
     2701 atggtcagcc tccgcattcg gtcatgccgt atgcgttggc gagtttgttc gcttccgcga
     2761 acggatcccc gttgccttgc agggaggaag gatctgactt gccctcctcg agagccttgt
     2821 tggccgcatc gaggatcgcg ctgatctggt cctcttcgcc ctccggaacc ggaagcgcct
     2881 cgatgtcgtt gatctgggac tccacctgag gcagcacggt gtcattgatg aacgagtcga
     2941 catccgcctg ggtcggctgg ccgccggaga acgcttcctt ctcggccgcg tcgatcgtct
     3001 tgttgctctc ggcgcagatc tcgttcgcct gggcgacgaa ctccgccttg gtgatcgatt
     3061 ccgaagaatc gtcggacgag ctgtcatcgg acccgcaggc tgtcacgccc agcgagacca
     3121 taaggagcaa cgaacagacg ccgatcttcc caaccttcaa tgcactcata ctcccccttc
     3181 ggttgactgg tctcagttga aactaaccgc aaatgtgcga cgggagtgaa atgaaccggg
     3241 atgacgagcg aatcatcgag gggatgcggc gacagtccga aggcatttca aagtcgggtg
     3301 cgggtggggc cgcgaacctg gagcggctcg gctggaaggc cgggttcggc accggggcga
     3361 cgatggcact gatcgggacc agccagccac tggcagggtt cctgacgaag gcgacgctgc
     3421 tcgactcggg ggcattcgtc caggtcggcg gatggggcgt gcctctgttc gaggccgagg
     3481 tcgcggtccg gatcgattcg gaaatcggtg ccggcgcgac tgccggagag gtgacggcgg
     3541 cggccggtgc ggtggcggcg gcgatcgagc tggtcgactt cggatcgatc gagagcgtcg
     3601 aggagatcct cgccgggaac atcttccatc gtcatgtgtt gcttggtgag ttcgtcgagc
     3661 tccctccgga cgggctcgac ggggtcagca tttcggtggc cgccaacgga gaggcggcgg
     3721 aggcctccga cccccgggag gccctcggtg atctcgggca ggtggtggcc gcactggccg
     3781 accaggccgc cctgaccggg gagtcgatca agccgggaga cgtggtgatc accggtgccg
     3841 ccatccctcc cgccccggtc agcccggggg acgattacgg ggtcgagatc accggcggca
     3901 gctcggtcac ggtcaaactg gtgtgaacga acgcgtggga accaactgca ttgaagttat
     3961 tcgttccatg tgcggataac ttcaacccag aagtccggac ctcacgggtc agcctgaaaa
     4021 tcggggcgac tggatttgaa ccagcgacct ttcgaccccc agtcgaatgc gctaccaaac
     4081 tgcgccacgc cccgtgcgtg accggggact ttagcgggag acggcttggc gccgctgtac
     4141 ggagcatgcg ccccgggacc gggtgaagcg ggcgacggga atcgaacccg ccctaggagc
     4201 ttggaaggct cctgtgcaac caatacacct cgcccgcaga gaaatcgcga ccaccaatta
     4261 taggggccga atcctgactc cgatccgtcg aaaatcgacc cgggaagcgg ggcacccagg
     4321 actcgaacct ggaaccttcg gctttggagg ccgctgctca gccaattgag ctagtgcccc
     4381 ggaggatctg atcctagcgc ctcaaaggcg taggagtcac ccccgaaaaa cgctcattcc
     4441 atccgatccc ggtgcgaaca tatgttcgtg taccggatcc attcggaagc aactcgagaa
     4501 gaagtcaggt cgctgatcga ctcgggcgtt tcggatttcg agatcgcgaa gaggaccggt
     4561 gtcggaagat ccactgtcct taggtggagg caccatggcg ttccatctcc gcgggtgccc
     4621 atgacgccgg agatagtgga tcgggccgag tgggggccca aggaacgcca gatctacgct
     4681 tacttgctcg gccagtacct gggagacggt tacatcggaa cccacgggtc aacccactca
     4741 ctcgtgatcg cttgcgacat ccagtactgc cgaatactca cccaagtggt cgaggctgtc
     4801 ggcacgttcg ccccgaagcc gccgtcagtg ctgcgcccca aaggcaccaa cggcgtaagg
     4861 gtcacttcct attggcgcag ctggcctcag ctctttcctc agcatgggcc ggggagcaag
     4921 ctggatcgga agattgagct ggtggattgg caggtggaga ttgtggacga gcatccgaag
     4981 gctttcctta gggggctgat tcattcggat gggtcgcggt gcatgaacac cttcaagatg
     5041 gccttgaagg acgggccgaa ggagtactcg tacccccgct acttcttcac caactactcg
     5101 gccgacatcc aggcgatctt ctgccggacc tgtgaccggc tggggatccg ttggagccgg
     5161 tcgaactggc ggaacatctc gatctcgcac cggaccagcg tcgcgctgct cgatgaattt
     5221 gtaggaccca aggattgacg cctatgctga ggaactgaga tgagcgaccg actcaaaatc
     5281 gtttccgtgc tggggagcgt cacggctccg gggcggatgc accgggcctt gaacgaggcg
     5341 ctcgaccggg ccgggacggg tgctggcggt gcgacgggcg acggtgaggt cgctgttgag
     5401 cggattgatt tggccgagaa gacggtcacc ttcgccgacg gcaccccgct cgaccagctg
     5461 ggtgatgact cggccgccgt gatcgggcag atcgcccggg ccgacgccgt ggtgctcgcc
     5521 acgcccgtct accggggctc gatgaccggg gcgctcaaga acctgctcga cctgaccccg
     5581 gtcgaggcgt tgcggggcaa gccggttctg atcgtggcga tgggagcgac cgaccatcac
     5641 tcgctcgggg tcgaacggca cctgcgggac gtactcagct tcttcggtgc gctgccagtt
     5701 cctgtcgacg tctacctgac ctcggccgat ttcacggacg gagtgccggg cgatgacgca
     5761 gcccggcgac tcgaccaggg cctcgatggg ttgctggcgc tgacccgggc gaccggcggc
     5821 ctcggcgagt tcggccccga cccgctgatc acccggctct cgcggccgca gagctgagca
     5881 ggtcgcgaac cccaggtcgc cgcgaccctg gcggggaatc ggcgagacca gcaatgcgcg
     5941 ccccgtcgcc cggctcagcc ggggatcagg gtcagcggct cgtccgggga gacctggtcc
     6001 gggtcgttga tcccggcgcc ggggggtggc gggatcgggc cgaggcggat cgggtcgtcg
     6061 gtctcgacca tccagcgatc gagccgggag cgcatgtcaa ccagaatgtc ggcgctgccg
     6121 gggtcaccgg ccaggttgtt ctcctcgaac gggtcgaggt agaggtcaaa cagctgctct
     6181 cgggcgacct cgcggtcacc ccaccccatt gcgatcaaca ggtccttggt caggctgtcg
     6241 tcgcaattcg cgaggaccgg ccgcttgcga tcgtcgaacc ggcggatgta cttccagcga
     6301 ttggtccgca ccgccctttg cggctcgtag gcggcgtggt aggtcacctc ggagaacagc
     6361 tcttcgtgaa gagcggcgga ttccccgctg accaggggca tcagtgacct gccctgcagg
     6421 aaatccgggg gcggcacccc ggccagttcg cagaccgtcg ggtagatgtc gaggtgggag
     6481 accatcgaat cgatcacctt gccgccggag aaaccgttcg ggcctcgcat cagcatcatc
     6541 acgccgatcc cgccgtcgag cagggtcgcc ttcgccttgg ggaaggcgag gccgtggtcg
     6601 gtggtgcaga tgaccagggt ccggtcggcc aggccggtcg cgtggagacc gttgaggagt
     6661 gcaccgatgc cctggtccag cgaacgcacg ctctccttga aggaggccat gtcactgcgc
     6721 gtttcggccg tgtccggcag gttctccggc ggcagcgaat aaagcgagtc gcggaccgag
     6781 gtcggggccg cgaagtcacg atgggtttcg aagaagccga ccgacatgaa gaagggctca
     6841 cttgtccggc ggatcgcttc gagggcgctg ggggcaaccc gcttggcgta gttgtcgctc
     6901 agctcgatca cctcgtcgta accgatcacg tgcgggtcgc gggagatgtg ctgctcgccg
     6961 accaggatgg tccggtagcc gccctccttc aggggatgaa ccatcgtctg cgagtagtcg
     7021 ttcagcttcc agccccggtg ggcgagcccc atcattccgt tgttgtggca atgctggccg
     7081 gtcaggaggg ccgcccggct ccccgagcag gtcggggcgg cgcagaaggc ccgccggaaa
     7141 gtgatcccct ggtcggccag cagctgcatg ttcggggtca ggacgtgatg gccgtagggc
     7201 tggacatagc ggcccgtgtc atgggaatgc acatagatga tgttggggcg gtccgtcatt
     7261 ggcgaagtcc ggagccgctc agtcccggcc ggaaggggtc gggaccaggt cgaactcggc
     7321 gccgaggcgg atgtcgctgt agcgccgggc ccagtcgtcg ttgatgccat cggactggcg
     7381 ccgcatcctc gtctccagat tgaggtcatc cggggcctcg atgcccagcc tctcgagcac
     7441 gttgcgggtg ctcggcccga tgtccgaggc gaagttctcg tagaggatga agacgggctt
     7501 gatccgggaa tgctcaaaga acgcgtccca ggcagcttcc tgctggatca gctggtcgag
     7561 caggtgcttg atcgccccgt agtgaaagcg gagcgccggg cggtggtcgt tgacgaaacc
     7621 cttgtaggag gcgtcgatgt cttccgcgtc agcgctggag ctcgcggtgg tttcatcgcg
     7681 ccaggtagcg gtctggacgg ccttccagag cgaaacggcc tggcggatct tgtttgccct
     7741 gaccacccgc acgaaggtca gctcggggaa gacctcggag aggatctccg ccatcggtac
     7801 ttccctgaat tcggggatgt tgcggagcag cgagacgaag tcctcgacgt agccccacat
     7861 cagctttgcc ccgaagaccc cgttgtcggt ggtgccgaca tcgaagaccc agtcgagata
     7921 acggtcgtag gcggtgcggc tccagagcgg cgaacgcggc gggggattga agtcctgggc
     7981 ccgctcggcg aggtgatcga agatcgtccg gtcccccacc ccgtggaaat actcctccgg
     8041 ctgacgcggc accccgctgc ttctgagtgc ctcgaagtat tcctcgggcc ggcccgcgac
     8101 cccggttctg atcagggtct ggcagaccag ggtgctcccg cttcggggcg tcgcacagac
     8161 caggtatgag cgttcgggct tactcatagt tgaccagttt actcaggaat cttcgttggt
     8221 caaggtgaca gtaccccgtc ccccctcccg gcaataatgg tgatgcgaat cgcctttttg
     8281 ttcatgagca acgccgccac agatgccgcc ccgcccgttc ccgagagtgt cctcgtcgga
     8341 ggcgtcgggc tgggcgcgat acccaagcaa cagcgcggga tcgaggtgcg ggaccggctc
     8401 tacgaagcgg cggtggccga gatcgacgcg aagggcatcg aggagtcccg ggtcgagaac
     8461 atcgttgccg aggcgggcac cagctggggc accttctttc gctacttccc ccggaaggaa
     8521 gacatcttcc tgcacgcctc cgtgaggaac ttccgggagt acctgaagcc ggccttcgac
     8581 gaggtggtcg ccgacgactc cgcctcgatc aaggaaggtg cgcgcagcat cattctgctg
     8641 gtcatggaac cacgggtcag cccccgcttt cacgccgaga tgctggccga ggtccttcgc
     8701 cacccggccc gtttcgccgc gatcctcggt gagggcgaac tgccgctggc ggtgctcatg
     8761 gccgggctgc tggtcgaagg tcagaagcgg ggcgaagtga cagccgaggc gccggtgccg
     8821 gtctgcgcgg cagtcctgct ggcgggagtc atgttctcgg cctcgaccgt gctccaggcg
     8881 gtggccgaag gcacccggcc cgggtccgac atcgccgagg tagccaacca ggcattcgac
     8941 cttgcctggt ccggaatcgg tgcctgacct gccgaactga aaagatcaac cggggtcgcc
     9001 ggtcggcggc cattcaagcg gacgtggcgg aactggtaga cgcgtcaggt ttaggtcctg
     9061 gtgcccgcga gggtgtggag gttcgagtcc tctcgtccgc attggtccgg gtccgggagc
     9121 gagtcccggt ccccggaggg ctgctcagct gtcggtgccg aagctctcgt cgatgaaggg
     9181 gccgatcaac cggccgaccc tggcggccgt ctcgtcacgg gaaccggtcg gtgcagtcac
     9241 atggctgatc gccagccgga cgagattctc cgagatcagg tttgcgtcat cggccgagta
     9301 tcccggccag gtctcgacga tgatcacgcc gatccgttcg gccagccagc tgatcatcgg
     9361 tgtgcccctc gtggtgacga actggagcat cccgccggtc ccgtcatcgc tcaggagtgt
     9421 ggcgatcacc ggatctccgc tggcgatcag caggaaccgc tcgaaggcca cctcgaccgc
     9481 cgcccgagga tcatccgact ttgcacggat cgcctcctcg acaccgtcga ggaacttccc
     9541 ggcttcatga agggcgtagg tgagaccgaa ctcgttccgg ctgccgaact cgttgtaaag
     9601 ggtctgccgg ctgaccccgg cctcgaccgc gatgtcggcc atcgtgatct cggaccaggg
     9661 ccgcacttca agctttccgc gggccgccct gaacacggtt tcgcgcagca gctcgcgggt
     9721 tgcctcgcga tatggacttg gactcctccc catgtgcgac atcgtcgcag cccggccggt
     9781 caatcgtccg gctcgaaatc cactttgtcc cggacaccgc aatccgggca ggcccagctg
     9841 tccgggaccg cctcccaggc ggtgcccggc ggaaaccctt cgcgggggtg acccttttcc
     9901 tcgtcgtaca cgtacccgca gtccgggcag acccagcgac tcatgaggcg ctcccgtaac
     9961 gggcctcgat ccggcggcgg gcccggggct gcatgttgat ccggctcacg tctccgtcgt
    10021 agtgggcgag caggcgcggg tccatgaccc gcctccagac cgggggaaac agggcgagga
    10081 cgatcatccc ggcgtatccg gtcggcagct ggggcgcttc ctcggcatgc ctcagcgcct
    10141 ggtagcgacg ggtcggatgg gtgtggtggt cgctgtgacg ctgaaggtga aacagcagga
    10201 cgttggaagc gacgttgttg ctgttccagc tgtgctgctg ctggcacctt tcgtatcgac
    10261 cgtcatcctt gcgctgtcgc aaaaggccgt agtgctccag gtaattgacg acctcgagca
    10321 gggaaaagcc gaccacggcc tggatgatca gatagggcag gacgaccggc ccgaaggcga
    10381 ccgcgagacc gccgaagagg agaacggtca tcgcccacgc gctgaggatg tcgttcttgt
    10441 aactccaatg ggaaacaccc agccggtcca gccgtgcggc ttcgatcccc caggccgagc
    10501 gcaggctgcc cgagacggtt cggggcaaaa aggcccagaa gctctcgccc atccgcgagc
    10561 tggccgggtc ctcgggcgtc gcaacgcgga cgtgatgacc gcggttgtgt tcgatgaaga
    10621 aatggccata gccggtctgg gcgagcgcga cccggcccag ccagcgttcc atcgcttcgc
    10681 gcttgtgacc gagctcgtgg gccgtgttga tggcaattcc gccaaccacg cccatggtca
    10741 gggtcagtcc gatcgattcg aaaaccgaga ggccgccatc ggcccactgc cagcaggcaa
    10801 agaccagccc ggcgtactgg accgggatga acatgtaggt gcaccagcgg tagtaacggt
    10861 cctgctccag ccatttgagg acgctgtcgg ggggattggt cgggtcgaga ccgatcacgg
    10921 tgtcgagcag gggaaacagc ccgaagacga ccagaggcgc cgagtaccag aagatcccca
    10981 gtccggtcgc ttcgaccagt tgccaggaaa tgaagggcaa gagggggacg aggatgccga
    11041 gcagccaggc gtagcgcttg ccgtcgcgcc agccggccgg gtttagatcg acttcatcgt
    11101 ggtgatggtg gtgatgatga cccggcgctt cttcagcgac cggtcccgca gcaggttgct
    11161 tgtcagtaag ttcgtccatg tcgctttact ttacacaaaa ttgctttttg taaacatcag
    11221 accttgatcc gggctgcgga catccgggtt tgaatctatg atcggctcat ggcggtagga
    11281 gaggcaaggc ccggttacga gctgtggcgc ccgaaccggg agaaagcgga gtgcctgacc
    11341 acgcggttca tcgcggtggt gctgctggtc gcatcggcag ccttgatgct gatcgtgact
    11401 ctcgccggat gggacgtcct ggtcggcggg cagacctacg ggatcatctc cctgatcttc
    11461 gtcggggttt acatcctgct ggcggtaatg atctggcgct ggagccgcgg cgcgctcaca
    11521 ctgaccatgg ccctttcggt tctgctgggg atcttctgcg cggtcggagc tccgacctgg
    11581 tttgcccggg acaaggccgg cttcaccgaa ccgggtctgc cctccgatct gattggcctg
    11641 ctggttctcc tgctcatccc ggtacagatc gcagtgcttc tgacctgcgc gatcggattc
    11701 cggcaggaat ggcacgtcga ggaagagcgc ccgatcagct ccggggatgc ggacgcgggc
    11761 ggcgaccagg tcgagtccgg ctcactggcc ggcgcctcct gagccgagcg gcataaactt
    11821 gagcgtcccg gcggcggtgg cggaactggt atacgcgacg gactcaaaat ccgttgcccg
    11881 caagggcgtg tgggttcgat tcccacccgc cgcataggga gaaaccgtgg aagcagccga
    11941 agaaaagcca acctcccccc gggaaaagga cgagaagacc gacgcgctcg ccagcggccc
    12001 gacgggagtc gcggggagcg acctcgagcg acgggcagca gcgaggctgg tcgaagagct
    12061 tgagagcaag ggccgctcgg cgcgggtcca ggaaatgaag attcccgcct cggagtcggc
    12121 cacgatcacg cttcacgccg cgctcaccct ggcggccacc ctgatcggga tgaaatggcc
    12181 ggcgatcggc gcgacaatct gcctgatcgc ggccttctcc ttctattccg agcgcggtct
    12241 cggcctgcgc ctgatcggcc gcgcgattcc ggggcgccgg accggcaacg tgctctcccc
    12301 tcctcccggc ccggcctggg aagaggtcga ggtgatcctc gcaaccggat atgacgtacc
    12361 cgactcctac ccggtcggcg aatggctttc gcgccggttc tcggggcgcc tcacgaccga
    12421 ccggatcctg ttttacggcg gcatgatcgg aaccttcgcc gcgttgatga tgcgggccgt
    12481 gggcatcgag gacaccgccc tcagcatctt ccagaccctg accgcggcga tcccgctcgc
    12541 cgcaatcgcc gctcaggtcg accgccacct ggccgggacc ccggtcgcca cgcaggagga
    12601 tctcgccccg gcccgcgacg tgatggccgc tgtccgggaa gccgacgacc agtcggaagg
    12661 cgattcgggc gtggcggtct gcctgttcgg ctcggagtat tcgtcagccg ccggcgcctc
    12721 cgccttcttc gccgacagcc ggctgaagct caagcaggga gcggctttgg tcaatctggt
    12781 gcggggcgcc cgtgcctcgg ggcccgaggt gaccggcagc gaaggggatt tgatgccgac
    12841 ccgcatgaat ccggaactgg cggccggcag tccgctgaaa ccgaagatgg tcgttctgcg
    12901 gaaccagacc gcagccacga ttgcccggcg gcggggcctg cgcgcgacca cggtggttgg
    12961 ccggggcgaa gccggagtcg acgtcctgct cgacgcttcg gaaggcgccc tgcccgacga
    13021 ggaggaaaag gaatgagtcc cgaaccgctc tggtcgccgg atcctgaagc gatcgagagt
    13081 tcgcagctcg tcgcctgggc ccactggctg aaggatcgtg acctgggacc cggaaccgcg
    13141 gagtacgccg atctctggca atggtcaacc gaccagcccg aggaattctg gcgttcgatc
    13201 tgggattact tcgaggttct ccatgacggc gaaccggccg cggtcctctc cggccacgag
    13261 atgcccggcg ccagatggtt cgagggcacc agtctcaact acgccgaaca catcttccgc
    13321 gaccgcgatc cggaagcggc agccgtcttt gccgcgtcgg aggtgcgggc gctcgaaaca
    13381 gtgacctggg gtgagttgcg aagccgggtg gccgactgcg cggcgggact gaaggcgctg
    13441 ggcgtcgggc ccggcgaccg ggttgccggc taccttccga attcgcacga gacgatcgtt
    13501 gccttcctcg ccgttgccag cctgggcgcg gtctggtcga gctgctcccc ggatttcggt
    13561 gccggggccg tgaccgaccg tctggcccag atcgagccca gagtcctgct cgcggtcgac
    13621 ggctacaact acggcggcaa gggatttgac cgtcgcggcg tggtttccga tctggttgcc
    13681 ggtatgccca cggtcgagca ggtcgtgatc ctgccctacc tggaaccgga tcccgacctg
    13741 accccgctcc ctggcgccat tacctggaac gaactgcttt caaaggatcc cggggccgag
    13801 ttgacgttta cccgggtgcc attcgactcc cccctgtgga tcctctactc ctccggcacc
    13861 accgggctgc cgaagccgat cgtccagagc c
//
LOCUS       DAOQKN010000050        13857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_2074_length_13857_cov_5.248732, whole genome shotgun sequence.
ACCESSION   DAOQKN010000050 DAOQKN010000000
VERSION     DAOQKN010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 13857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13857
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2074_length_13857_cov_5.248732"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1139)
                     /locus_tag="PLJ64_05045"
     CDS             complement(<1..1139)
                     /locus_tag="PLJ64_05045"
                     /inference="COORDINATES: protein motif:HMM:NF012975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease catalytic domain-containing
                     protein"
                     /protein_id="HNC92884.1"
                     /translation="MADRRSDRAALPRKVALLERKGRFTSARPLFERGQPIALERKAR
                     TREGQIAWVEFGAGRARLIRDLGRIDNARDVSEALTLERLGRRGFPQKVEEAAEQAAA
                     SPIEAARQDLTGLPTFTVDPATARDFDDAVSARREGDGLRLWIHIADVSAFVRPGSIL
                     EKEAFLRANSTYVPTSVEPMLPHSLSSGVCSLSPGDERLAVTSEMLLSGSGEVTSASF
                     YRSRIRSDARLDYDQLDQIFAGRESAPEIVAEQLDLARKAAAILAELRKGNALEVNSA
                     EPSFEFDDEGNVTRAVAERATEAHRLIEQLMVLTNEQVAGLTVNRGIPSVYRVHEMPD
                     PDRVERMLAQLASLELPAPPVPSGMGPSTAGQLAVEASRSVAKEAA"
     gene            complement(1132..2322)
                     /locus_tag="PLJ64_05050"
     CDS             complement(1132..2322)
                     /locus_tag="PLJ64_05050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNC92885.1"
                     /translation="MKTATVGVWAVIGGMLIGMWAGGHPDNLPDSLREIFVADEVQQS
                     VSDQAAELIHDNYFRATDPDQVQDSSINGMVRQIRRQYHDRFSHYFTPKQNEQLSESL
                     QGSFSGVGMTVGANAKKGLEVGYVFRKSPADEAGLKAGDVIVEVNGESIRGEDVDLIV
                     AKIKGPEGTDVTLGVRKAGKGPVRDFKLTREEIRVPITANRIRKVDGKSLGYVQLTTF
                     SQGAGLALRRAVERVIGKGAEGIVIDLRDNGGGLLEEAVFTSSVFLDEGDVVVETRSR
                     SQGNTTYRAVKGKVDSPPLVVLVNRNTASAAEILAAALQTDLDTPVVGTRTFGKGLFQ
                     QVLSLENGGALDLSVGEFLTADGVSLAGKGLKPDVYAPLPKGARRDVQLDRAFQTLSG
                     EVDG"
     gene            complement(2372..3286)
                     /locus_tag="PLJ64_05055"
     CDS             complement(2372..3286)
                     /locus_tag="PLJ64_05055"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HNC92886.1"
                     /translation="MGKIFFFTGEALRAIKRNVAPTTAAVVTTVLTALLLGVLIPVFQ
                     TAQSKGEDVRGQLELRTFLYDDATKAETQALMVKLEGLPHVESVTLLTKADAKKEFLA
                     SDFGKQNPDALKQLGGKNPLPANFVIKPDDASNLEGIRNAIQPVGAKGDPQYISPIIA
                     SVDDRQEDARDIEQVTGALKLILTVITVLLICASLLLIGNTIRLSIYTRRREIEVMRL
                     VGATRWFIRWPFMIEGVVVGILGGAIAILVLWIGKVTVVDPLSDKFALMNAQDSMSFA
                     ALVGCLFIAAILVSAIGSGLTMRRFLKV"
     gene            complement(3286..4167)
                     /gene="ftsE"
                     /locus_tag="PLJ64_05060"
     CDS             complement(3286..4167)
                     /gene="ftsE"
                     /locus_tag="PLJ64_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HNC92887.1"
                     /translation="MAFGKKKRESAGAELEAARARKQAERPRKGGAPIIEMRDVSKVY
                     PGGHLAVDRVSLAINRGEFVFLVGPTGCGKSTLIKMLIRELEPTDGTISIAGRDINQL
                     SEKKIPQLRRNIGTVFQDFKLLPSRTVYDNVAYALQVIGASRAEIRQKVPETLRLVGL
                     SAKLHNYPDELSGGEQQRVSVARAFVNHPPLLLADEPSGNLDPVTSVGIMQLLYRINK
                     TGTTVVVVTHDREMVDRMRRRVVELYEGRVIRDEVAGGYTEESTTEFGVRMRAEMGVG
                     VEGHTNGNSGNSGKGAH"
     gene            4331..5491
                     /locus_tag="PLJ64_05065"
     CDS             4331..5491
                     /locus_tag="PLJ64_05065"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HNC92888.1"
                     /translation="MSRAAHLANRAALAGCGLLLAILLAAFLLPSSPAAAGGEPSSSE
                     SGATASIVNGKATTIGQWPWQVALTVSSRIAPRASTSWRFFCGGSVLAPRLVITAGHC
                     VADLNRGQIRKIEIVSGRTRLNSNRGQVARVTGLRMPVNASGKRRFRGLAGAADWDVA
                     LLTLASPLSAEPIKLAGPDEAAAWSPGQVAWTTGWGITKAFSQRVPARLQVARQVLMG
                     NGLCHRADGVAYKSRRMICMGAPRAHASACNGDSGGPLVVETGAGYRLVGLTSYGDGA
                     CRGFVPSVDTRVSGRQIRNWVARTAMNLTGRNVVGSGGTPRPAPDWCKVPDVFRLRQS
                     QAKARLEAAGCRLGRVRTDYYSVGQAGRIVGSRLAPGWLAPPGFKVGVWVAP"
     gene            5509..5778
                     /locus_tag="PLJ64_05070"
     CDS             5509..5778
                     /locus_tag="PLJ64_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92889.1"
                     /translation="MNRQRLTGLTVTLLATALMALGLAACGSDDDGSGSSGTTGTEVS
                     QQMNGKKNEVTPDSKAPASAGNKDKNAPDDAISNRPGGPNKPASP"
     gene            complement(5775..6812)
                     /gene="nadA"
                     /locus_tag="PLJ64_05075"
     CDS             complement(5775..6812)
                     /gene="nadA"
                     /locus_tag="PLJ64_05075"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNC92890.1"
                     /translation="MPTELPVLQASDVPPPLDPAEIKELSAEIRELAAERNAIILAHN
                     YELPEVQDVADYIGDSLGLSREAEKADAEVVEFCGVHFMAETASILSPEKKVLIPDLE
                     AGCSLSDSINADQLREWKAKFPGAMVVMYVNTSAEVKAETDYCCTSSNAVKVVEHIWA
                     EHGEDTEILFGPDMWLGAFVERETGLIEDPERRSRFHVWDGECHVHAGIRPDDIARTR
                     AEHPGAEFLIHPECGCSTQAMEYVATGDIDPEGVHMLSTSGMIEHVRENPEGEYIVAT
                     ENGMLYPMKQAVPGARLIEANRMAYCKYMKMITLPKLRDSLRDMKFEVKVDEALAEKA
                     RLPIERMVSIG"
     gene            complement(6823..7683)
                     /gene="nadC"
                     /locus_tag="PLJ64_05080"
     CDS             complement(6823..7683)
                     /gene="nadC"
                     /locus_tag="PLJ64_05080"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNC92891.1"
                     /translation="MNHLPEGTAELIARALAEDIGPGDVTANATVPADLAAQAHIVLK
                     QPGVIYGLDVVEEVFRQVGVTDIDRTVVEGHWHDSVPRDILLINGQARAILAGERVAL
                     NFLGHLSGIATLTARYVDAVGGTGTRILDTRKTLPGLRALEKQAVSWGGGINHRFGLF
                     DAVLIKENHVALAGGVREAIESCRRESPDLLIEVEAEDLDQVAEAIEAGADRILLDNM
                     DIESLKRAVDLRESGGPGTELEASGGMNLETVRAVAETGVDFISIGALTHSAPQLDVS
                     LLLDPVNRLP"
     gene            7707..8216
                     /locus_tag="PLJ64_05085"
     CDS             7707..8216
                     /locus_tag="PLJ64_05085"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNC92892.1"
                     /translation="MDSFTHTAFDTPLGPVALAATETGVVRCNLPGSDPDALVEEVAT
                     KTGLTPVEGGRTVDQAADLVIAFLEGDLHDFDLELDWQLVGGFHREVLRATRTIPWGE
                     TASYGDVAALAGAPGAARAAGTALSRNPIALIVPCHRVIKADGTTGGYGGGKAGTALK
                     QRLLDLERP"
     gene            complement(8277..9356)
                     /gene="prfB"
                     /locus_tag="PLJ64_05090"
     CDS             complement(8277..9356)
                     /gene="prfB"
                     /locus_tag="PLJ64_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157591830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNC92893.1"
                     /translation="MGARLAAIDEHVALLADYLDPEGLEAEVAEFEQVMQQPGFWDDQ
                     DKAASVSADHARVQRKLEGFRALSSEAGDLAEMAEMAELDEEIAAELEEQMSSVEARL
                     EELEEARLFSGEYDHGDAIVSVHAGAGGTDSQDWAEILLRMYLRWAERRGFSVEMREA
                     SEGEEAGLKSATFLLKGENAYGLIAAERGVHRLVRISPFDSSSRRHTSFAQVDVAPVV
                     DEDVEIEIDDAEIRVDTYRASGAGGQHVNKTDSAVRITHEPTGVVVQCQNERSQTQNK
                     AVAMQMLKSKLVELEERKRAEAVNQERGEVKDVAWGSQIRSYVLQPYTMVKDHRTDFE
                     VGDAQRVLDGDIDGFIREYLNRTAG"
     gene            9451..10566
                     /locus_tag="PLJ64_05095"
     CDS             9451..10566
                     /locus_tag="PLJ64_05095"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HNC92894.1"
                     /translation="MTSARGRIRVMIIATAVALISTLGPAGSATASPDASASIINGKP
                     ASIGDWPWQVAIASSTSKRPGQSPFYRTFCGGSIVAPTVILTASHCALDMYKGEVGDY
                     TVISGRTNLNNQAAGQEVFVTGIYFPRTSNGKILYKTRQAWDVSLLTLEEPLAGQPVK
                     LLGPDEHSILKPGRRLIETGWGSTITGRDVYPKTLKMGGTNIQPPSVCHGLLGSRYFK
                     PSLQVCLGDAKANQANCYGDSGGPAVVATSAGYRQVGVTSFGTADNCAGLIPNVDVLV
                     GGGPVRDWIQAGVQVLGGVDPVGSGGTAGAISGLCHMPGVRGLSIARARHLIKASGCR
                     KVRVIHRGRGNRVSRLPALKGWLWDASKPIRLLAGGR"
     gene            10588..11556
                     /locus_tag="PLJ64_05100"
     CDS             10588..11556
                     /locus_tag="PLJ64_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNC92895.1"
                     /translation="MSEASAAGGTDSGGEARRKRLIRVISVSITVAVLTIGMKLAAWL
                     LTGSVGLLSDAAESVVNLVAAGVALIVILWSTRPADEDHTYGHEKADYLSAGLEGALI
                     LLAAVTIAYAAIERLIHPVELNSVGIGLAISAAASVLNLLAARLLIRTGEEETSLVLV
                     ADGRHLMADVITSAGVIVGVALVWLTGLERLDPVIALVVAANIIRTGTGLVSDSMSGL
                     MDKALPEDELQKVSQVLDGFESDDIRFHALRTRRAGRRTFISIHVLVPGNWSVKQGHD
                     LLERVEEDLRACFEQTTVFTHLEPIEDPVSFADTGLDRTSNPHTGG"
     gene            complement(11586..11969)
                     /locus_tag="PLJ64_05105"
     CDS             complement(11586..11969)
                     /locus_tag="PLJ64_05105"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNC92896.1"
                     /translation="MRPVTPEEKTVREFVATFNRSELDEFVELLDPEVEIHGMRGLRK
                     GRDEARAWATKKPGGVQQTVLIENCRVAGDRVLLGVKREWRWEEDDELAYTDELAWLL
                     ELRGARVLSWKPFEDPEAARTAFEA"
     gene            complement(12030..>13857)
                     /locus_tag="PLJ64_05110"
     CDS             complement(12030..>13857)
                     /locus_tag="PLJ64_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HNC92897.1"
                     /translation="LEGRRWSEGLHQAVEAKEGVAIREENQTLATITLQNYFRLYDKL
                     SGMTGTALTEAVEFMKIYKVPVVEVPTNRPISRDDQNDQIFKTKEGKWQAVIRELVAR
                     HETGQPILVGTVSVEVSEMISAELKKQGIEHVVLNAKPEHAEKEGETIAQAGRKGAVT
                     IATNMAGRGVDIKLGGDPEGMAHPQVAKQGLNPEDEGYNEAVEKIAAELKPQCDAEAE
                     EVKELGGLFICGTERHESRRIDNQLRGRAGRQGDPGASRFFLSAEDDVIRLFAGDRIY
                     KILDRLGPTDEEGHEMPLEAKMLTKTVENAQKKVEEQNFLIRKRVLEYDDVMNEQRRV
                     IYKYRGEILEGRDMSEIAREEIDGVIGRLVDEFMAGDIIEEWDLPGLDSQLHQIWPCA
                     IDVAGLPPESTEPEALKQDLCEDAQKAYDEREAEFGEEIMRYLERTLLMQVIDSRWRE
                     HLYDMDYLREGIHLRGFAQIDPLVAYKNEGYTMFQELMNGIWEEFSRLIYNVEIEIEP
                     DQNGSFAPPAEEPDEQTYDYSGGTMEAQPSALAEVAAGATPGPADAGGVPGVVPGGVP
                     NVGGAVDPANSATVVKTEREDIGRNDPCWCGSGKKYKKCHGQ"
BASE COUNT         2203 a         4616 c         4480 g         2558 t
ORIGIN      
        1 gccgcctctt tcgcgaccga acgggaagcc tccaccgcca gctgcccggc cgtgctcggc
       61 cccatgccgg aggggaccgg cggcgccggc aactccagcg aggccagctg ggcgagcatc
      121 cgttccaccc ggtcggggtc gggcatctcg tggacgcggt agacggaggg gatgccgcgg
      181 ttcacagtca gcccggccac ctgttcgttg gtcaggacca tcagctgctc gatcagccgg
      241 tgcgcttcgg ttgcccgctc ggccaccgcc ctggtcacgt tgccctcgtc atcgaattcg
      301 aaggaaggct cggccgagtt gacttcgagc gcgttcccct tccggagttc ggccaggatc
      361 gctgccgcct tccgggccag gtccagttgt tcggcgacga tttcgggggc gctttcgcgc
      421 ccggcgaaga tctggtcgag ctggtcgtag tcaagccggg catcggagcg gatccggctg
      481 cggtagaaac tggccgaagt tacctcgccg cttcccgaga gcagcatctc cgaagtgacg
      541 gccagccgct cgtcgccggg cgaaagggag cagacgccgg aactgaggct gtgcggaagc
      601 atcggttcga ccgaggtcgg tacataggtg ctgttggccc gcagaaaggc ttccttttcg
      661 agaatcgaac ccgggcgaac gaaggcggat acgtcggcga tgtgaatcca gagccgcagc
      721 ccgtccccct cgcggcgggc agaaaccgca tcgtcgaaat cccgggcggt ggccgggtcg
      781 accgtgaagg tcggcagacc ggtcaggtcc tgccgggccg cctcgatcgg tgaagcggcc
      841 gcctgctccg ctgcttcttc gaccttctgc gggaacccgc gccggcccaa acgctccagg
      901 gtcaacgcct cggagacgtc acgggcattg tcgatccggc cgaggtcccg gatcagccgg
      961 gctcggccgg caccgaactc gacccaggcg atctgaccct cccgggtcct cgccttgcgc
     1021 tccagcgcga tcggctggcc ccgctcgaag agcgggcggg cggaggtgaa ccggcccttc
     1081 ctttcaagca gggcgacttt tcggggaagc gctgcccgat cgctccgccg gtcagccatc
     1141 cacttcgccc gagagcgtct ggaatgcccg gtccagctgg acgtcgcgcc tggcaccctt
     1201 cgggagcggc gcgtagacgt cgggtttcaa tcccttgccg gccagcgaga caccgtccgc
     1261 agtgaggaat tcgccgacgc tgagatccag tgcgccgccg ttttcgaggc tgaggacctg
     1321 ctggaaaagg cccttgccga aggtcctggt cccgaccacc ggcgtgtcga ggtcggtctg
     1381 aagcgccgcc gcaaggatct ccgcggcgga ggcagtgttg cggttgacca gaaccaccag
     1441 cggcggcgag tccaccttgc ccttgaccgc ccggtaagta gtgttgccct gactccgcga
     1501 cctggtctcg acgacgacgt cgccttcatc gaggaagacg ctggaggtga agaccgcctc
     1561 ctcgagcagg ccgccgccgt tgtcgcggag gtcgatgacg atcccctcgg cccccttccc
     1621 gatcacccgc tcgaccgccc tccggagggc cagcccggca ccctggctga aggtcgtcag
     1681 ctggacgtag ccgagggact tcccgtccac cttgcggatc cggttggcgg tgatcggtac
     1741 ccggatttcc tcgcgggtga gtttgaagtc ccggaccggg cccttgcccg ccttgcggac
     1801 cccgagggtc acgtcggtcc cctccggccc cttgatcttc gccacgatca ggtcgacgtc
     1861 ttcaccccgg atcgactcgc cgttgacttc cacgatcaca tcaccggctt tcaggccggc
     1921 ctcgtcagcc ggggacttgc ggaagacgta accgacttcg agcccctttt tcgcattggc
     1981 accgacggtc atcccgacgc cgctgaagct gccctgaagc gactcgctga gctgctcgtt
     2041 ctgcttcggg gtgaagtagt gggagaagcg gtcgtggtac tggcgccgga tctgcctgac
     2101 catgccgttg atcgacgagt cctggacctg atccggatcg gtcgcccgga aatagttgtc
     2161 gtggatcagt tcggcagcct ggtcggaaac cgattgctgg acctcgtcgg cgacgaagat
     2221 ctcgcgcagc gaatcaggca ggttgtccgg atggccaccg gcccacatgc cgatgagcat
     2281 gccgccgatg acggcccaga cgccaacggt tgcggtcttc aactggccgc cggctgactc
     2341 tgaatgacgc ccggcagcgg gcccggccgg ctcagacctt caggaatctg cgcatggtca
     2401 gtccggaccc gatcgccgaa accaggatcg cggcgatgaa caggcagccg acgagggccg
     2461 caaaggacat cgagtcctgt gcgttcatca gggcgaactt gtccgagagg gggtcgacga
     2521 ctgtgacctt gccaatccac agcaccagta tcgcaatcgc gccgccgagg atgccgacca
     2581 cgaccccttc gatcatgaag ggccagcgga tgaaccagcg ggtcgcaccg accaggcgca
     2641 tgacctcgat ctcgcgccgg cgggtgtaga tggaaagccg gatcgtgttg ccaatcagga
     2701 gcagcgaggc acagatcagc agcacggtga tcacggtgag gatcagtttc agggcgccgg
     2761 tgacctgttc gatgtcgcgg gcgtcttcct ggcggtcatc gaccgaggcg atgatcggcg
     2821 agatgtactg ggggtcaccc ttggcgccga ccggctggat cgcgttgcgg atcccttcca
     2881 ggttggaagc gtcatcgggc ttgatcacga agttggccgg cagcgggttc ttgccaccga
     2941 gctgcttcag ggcgtcgggg ttctgcttgc cgaagtcact ggccaggaat tccttcttgg
     3001 cgtcggcctt ggtcagcagg gttaccgact ccacgtgtgg cagcccttcc agcttcacca
     3061 tcagggcctg ggtctcggcc ttggtcgcgt cgtcgtaaag gaaggtgcgc agctcgagct
     3121 ggccacgcac gtcctcgccc ttgctctggg cggtctggaa gacggggatc agcacaccga
     3181 gcagaagcgc ggtgagcacg gtggtcacta ccgcagccgt ggtcggcgcg acgttccgct
     3241 tgatcgcgcg gagcgcttcg ccggtgaaaa agaagatctt gcccatcagt gtgctccctt
     3301 gcccgaattt ccagagttgc cgttggtatg gccttcgacg ccaacgccca tctcggcccg
     3361 catgcggaca ccgaattcgg tggtcgactc ctcggtgtaa ccgccagcga cctcgtcgcg
     3421 gatgacccgg ccttcgtaga gctcgaccac gcggcggcgc atgcggtcga ccatttcccg
     3481 gtcgtgggtg accacgacga cggtggttcc ggtcttgttg atccggtaga gcagctgcat
     3541 gatgccgacc gaggtgaccg ggtcgaggtt gccgctcggc tcatcggcca gaagcagagg
     3601 cggatggttg acgaaggccc gggcgaccga gacgcgctgc tgctcgccgc cggaaagctc
     3661 gtcggggtag ttgtgaagct tggccgagag accgaccagg cgcagggtct caggcacctt
     3721 ctgccggatc tcggcccttg aagcgccgat cacctgaagc gcgtaggcga cgttgtcgta
     3781 aacggtgcgg ctcggcagca gcttgaagtc ctggaagacg gtgccgatgt tgcggcggag
     3841 ctgcggaatc ttcttctcgg aaagctggtt gatgtcacga ccggcgatcg agatcgtgcc
     3901 gtcggtgggc tcgagctcgc gaatcagcat cttgatcagg gtcgacttgc cgcagccggt
     3961 cgggccgacc aggaagacga actcgccacg gttgatcgcc agcgagaccc ggtcaacggc
     4021 aaggtggccg cccgggtaca ccttgctgac gtcacgcatc tcgatgatcg gggcgccacc
     4081 cttgcgcgga cgctcggcct gcttgcgggc gcgtgccgct tcgagctcgg cgcctgctga
     4141 ttctcttttc ttctttccga aagccatctg gttcgcccta ttccccccgg cccggggtaa
     4201 tccttcccct gacggggatc tcccacaaag ggcaaccccg gccaggttca tttcttcaga
     4261 gttagcgaaa cgttagcaat cgcttatatg actttggctg atcttaagcg cggccggtag
     4321 gattcctgca atgtccaggg ctgcccattt ggctaaccgg gccgccctcg caggttgcgg
     4381 cctcctcctc gcgatcctgc tcgcggcgtt ccttctgccc tcctccccgg ccgcggccgg
     4441 cggcgaacct tcaagcagtg aatccggcgc cacggcctcg atcgtcaacg gcaaggcaac
     4501 cacgatcggc cagtggccct ggcaggtcgc tctgaccgtg agctcgcgga ttgcgccgcg
     4561 ggcctcgacc agctggcgtt tcttttgcgg cggctcggtt ctcgcgccgc ggcttgtgat
     4621 cactgccggt cactgcgtcg ccgacctcaa ccgggggcag atccgcaaga tcgagatcgt
     4681 ttccggccgc acccgtctca attcgaaccg gggccaggtg gccagggtga ccgggctccg
     4741 catgccggtg aacgcctccg gaaagcggcg cttccgcggt cttgccggcg cggccgactg
     4801 ggacgtcgcc ctgctgaccc tcgcttcccc cctttcggcc gagccgatca agctggccgg
     4861 tccggacgag gcggcggcct ggtccccggg acaggtcgcc tggaccacag gctggggaat
     4921 caccaaggcc ttctcgcagc gggttccggc gaggctccag gtcgcccggc aggtcctgat
     4981 gggcaacggc ctctgccacc gggcagacgg cgtcgcgtac aagtcccgcc ggatgatctg
     5041 catgggagca ccccgggccc atgcctcggc ctgcaatgga gacagtggcg gacctctcgt
     5101 cgtcgagacc ggtgccggtt accggctggt cggcctgacc agttatggcg atggtgcctg
     5161 ccgcggattt gtcccctcgg ttgacacaag ggtctccggc aggcagatcc gcaactgggt
     5221 cgcacggacc gcgatgaacc tgaccggccg caacgtggtc ggcagcggtg gcactcccag
     5281 gcccgctccg gactggtgca aggtgccgga tgtcttccgg ctcaggcaga gccaggcgaa
     5341 ggccaggctc gaggccgcgg gttgccgcct cggccgggtc aggaccgact attacagcgt
     5401 cggccaggcc ggccggatcg tcggcagccg cctcgcaccc ggatggctgg ccccgcccgg
     5461 cttcaaggtg ggagtttggg ttgccccatg atcggctatg aaccgggcat gaaccgccaa
     5521 cggctgaccg gattgaccgt gaccctcctc gcaacggcct tgatggccct tggcctggcc
     5581 gcctgcggct ccgacgatga cggcagcgga agctccggca cgaccgggac ggaagtgagc
     5641 cagcagatga acgggaagaa gaacgaagtg acgccggaca gcaaggctcc ggccagcgcc
     5701 gggaacaagg acaagaacgc cccggacgac gctatctcga accgccccgg cggcccgaac
     5761 aagcccgcaa gcccttagcc gatactgacc atgcgctcga tcggcaggcg cgccttttcg
     5821 gccagcgcct cgtcgacctt gacttcgaac ttcatgtcgc gcagcgagtc gcgcagcttc
     5881 ggcagcgtga tcatcttcat gtacttgcag taggccatgc ggttggcctc gatcagcctg
     5941 gcccccggca ccgcctgctt catcgggtag agcatgccgt tctcggtggc gacgatgtac
     6001 tcgccttcgg ggttttcgcg gacgtgctcg atcatgcccg aggtcgagag catgtgcacg
     6061 ccctcggggt cgatgtcacc ggtcgccacg tactccatcg cctgggtcga gcagccgcat
     6121 tcgggatgga tcaggaactc ggcccccgga tgttcggccc gggtgcgggc gatgtcatcc
     6181 ggccggatgc cggcgtggac gtggcattcg ccgtcccaga catggaagcg tgaacgcctc
     6241 tcggggtcct cgatcagccc ggtctcgcgt tcaacgaagg caccgagcca catgtccggt
     6301 ccgaagagga tctcggtgtc ctcgccgtgc tcggcccaga tgtgctcgac caccttgacc
     6361 gcattcgagg aggtgcagca gtaatcggtt tccgccttta cttcggccga ggtgttgacg
     6421 tacatgacga ccatcgcccc ggggaatttc gccttccatt cccgcaactg atcggcgttg
     6481 atcgaatccg aaagcgagca gcctgcctcc agatcgggga tcagcacctt cttttccggc
     6541 gaaaggatcg acgccgtctc ggccatgaaa tggactccgc agaactcgac cacctcggca
     6601 tctgccttct ccgcctcgcg ggaaaggccc agcgagtcac cgatgtagtc cgccacgtcc
     6661 tggacttcgg gaagctcgta gttgtgggcg aggatgatcg cgttccgttc ggccgcgagt
     6721 tcgcggatct ccgcgctcag ttccttgatt tcggccggat cgagcggcgg cggcacatcc
     6781 gatgcctgga ggactggaag ttcggtgggc atggcaacaa ttctacggaa gccggttcac
     6841 gggatcgagc agcagcgaaa catccagttg cggagctgag tgggtcaggg cacctatcga
     6901 aatgaagtcc accccggtct cggctacggc ccggaccgtt tccaggttca tcccgcccga
     6961 ggcttcgagt tcggttcccg gaccgcccga ttcacgcagg tccacggccc gcttgagaga
     7021 ttcgatgtcc atgttgtcca gcaggatgcg gtcggcgccc gcttcgatcg cctcggcaac
     7081 ctgatcgaga tcctcggctt cgacttcgat gagcaggtcc ggggattcgc ggcggcagga
     7141 ctcgatcgct tcccggaccc cgcccgccag ggcgacgtgg ttctccttga tcagcaccgc
     7201 gtcgaagagt ccgaagcggt gattgatgcc accgccccag gagaccgcct gtttctccag
     7261 cgcccgcagg cccggcagtg tcttgcgcgt gtcgaggatc cgggtgccgg ttccgcccac
     7321 tgcgtcgacg tacctcgcgg tcagggtcgc aatgccggag aggtgaccga gaaagttcag
     7381 cgcaaccctt tccccggcga ggatcgccct ggcctggccg ttgatcaaca ggatgtcgcg
     7441 cgggaccgaa tcgtgccagt gaccttcgac cacggtgcga tcgatgtcgg tcaccccgac
     7501 ctgccggaag acttcctcga ccacgtccag tccgtagatg acccctggct gcttgaggac
     7561 gatgtgggcc tgcgccgcca ggtcggccgg aaccgtcgcg ttcgcggtca cgtcaccggg
     7621 gccgatgtct tcggccaggg cccgggcgat cagctccgcg gttccttcgg gcaggtgatt
     7681 catgcaggga gcctatgctc tccatggtgg attcgttcac tcacaccgct tttgacacgc
     7741 cgctgggccc ggtggccctg gccgccacgg aaacaggcgt ggttcgctgc aacctgccgg
     7801 gctcggaccc tgatgccctg gtcgaggaag tggccacgaa aaccgggctc accccggtcg
     7861 agggcggcag gacggtcgat caggcggcgg atctcgtgat cgcctttctc gagggcgatc
     7921 tgcatgactt cgatctcgag ctcgactggc aactggtcgg cggcttccac cgggaggtcc
     7981 tcagggcaac ccgaacgatc ccctggggtg agaccgccag ctacggcgat gtggcggccc
     8041 tggccggggc tcccggcgct gcccgcgccg ccgggaccgc cctctcgcga aacccgatcg
     8101 ccctgatcgt cccctgccac cgcgtaatca aggccgacgg aacaaccggc ggctacggcg
     8161 gcggcaaagc cggaaccgcc ttgaagcagc gcctcctcga cctcgagcgg ccgtgacggc
     8221 ggaggtgccg caggtttgca acctctgcgg tcgcaaacct gcgtcaccgg ccccgattat
     8281 ccggcggttc ggttgaggta ttcgcggatg aagccgtcga tgtcgccgtc gaggacccgc
     8341 tgggcgtcgc cgacttcgaa gtcggtgcgg tgatccttga ccatcgtgta gggctggagc
     8401 acgtaggagc ggatctggga gccccaggcg acatccttga cctcaccgcg ttcctgattg
     8461 accgcctcgg cccgcttccg ttcctcgagc tccaccagtt tcgacttgag catctgcatc
     8521 gcgaccgcct tgttctgggt ctgtgagcgc tcgttctggc actggaccac taccccggtc
     8581 ggctcatggg tgatccgcac cgccgagtcg gtcttgttga cgtgctggcc gccggcgccg
     8641 gaggcccggt aggtgtccac cctgatctcg gcgtcgtcga tctcgatctc cacgtcctcg
     8701 tcgacgaccg gggcaacgtc cacctgggcg aagcttgtgt gccggcggga cgaggaatcg
     8761 aagggcgaga tccggaccag ccggtggacg ccccgttccg cggcgatcag accgtaggcg
     8821 ttttcgccct tgagcaggaa ggtggccgat ttcagcccgg cttcttcgcc ttcggatgct
     8881 tcccgcatct cgaccgagaa gccgcgtcgc tcggcccagc gcaggtacat ccggagcagg
     8941 atctcggccc agtcctggga gtcggtcccg ccggccccgg cgtgaaccga gacgatcgcg
     9001 tcaccgtggt cgtattcgcc gctgaagagc ctcgcttctt ccagttcctc gagcctcgcc
     9061 tcgaccgagc tcatctgctc ttcgagttcg gccgcgattt cctcgtcgag ttcggccatt
     9121 tccgccatct cggcgaggtc gcccgcttcc gagctgaggg cacggaaacc ctcaagcttc
     9181 cgctggactc tcgcgtggtc ggctgaaacc gacgcggcct tgtcctggtc atcccagaat
     9241 cccggttgct gcattacttg ctcgaactcc gccacttcgg cttcgagccc ttcggggtcg
     9301 aggtaatcgg cgaggagtgc gacgtgttcg tcgatcgccg ccaggcgggc tccgatcgac
     9361 tcggtgttca gggcttcggc cacaaccgga gcctacccat cccgatgcct gcgaagatgt
     9421 accccaagca gagtgtcaac agggagaaac atgacttcag ccagagggcg catccgggta
     9481 atgatcatcg ccaccgccgt ggccctgatc tccacgctcg gaccggcagg atcagcgacg
     9541 gcttcgccgg acgcttcggc cagcatcatc aacggcaaac cggcttccat tggcgactgg
     9601 ccgtggcagg tcgcgatcgc ctcctcgacg agcaagcggc cgggccagtc ccccttctac
     9661 cggacctttt gcggtggttc gatagtcgca ccgaccgtga tcctcacggc cagtcactgt
     9721 gccctggaca tgtacaaggg cgaggttggc gactacacgg tcatctcggg caggaccaac
     9781 ctcaacaacc aggcagccgg gcaggaagtc ttcgtaaccg ggatctactt cccccggacc
     9841 agcaacggca agatcctcta caagacccgg caggcgtggg acgtttccct gctgacgctc
     9901 gaggagccgc ttgccggcca gccggtcaag ctgctcggac cggacgaaca ctcgatcctc
     9961 aaaccgggac gccgcctgat cgaaaccgga tggggcagca cgatcaccgg cagggacgtc
    10021 tacccgaaga cgctgaagat gggtggcacg aacatccagc ccccatcggt ctgccatggc
    10081 ctgctcggtt ccaggtattt caagccctcc ctccaggtct gtctcggtga tgcgaaggca
    10141 aaccaggcca attgctacgg cgacagcggc ggaccggcgg tcgtcgcaac ctcggccggc
    10201 taccggcagg tcggggtaac cagtttcggc accgcggaca actgcgccgg cctgatcccg
    10261 aatgttgacg tgctggtcgg cggcggcccg gtccgcgatt ggatccaggc aggagttcag
    10321 gtactcggtg gagtcgatcc ggtcggcagc ggcggaacgg cgggggcgat cagcggcctc
    10381 tgccacatgc cgggagtcag ggggctttca atcgcccggg cccgtcacct catcaaggcc
    10441 agcggctgca ggaaggtccg cgtgatccac cgcggccggg gcaacagggt ttcgaggctc
    10501 cccgccctca aaggctggct ttgggatgcc agcaagccga ttaggcttct ggctggtggc
    10561 aggtaacgaa cctgaaagca gttccacgtg agtgaggcat ccgccgcggg cgggacggac
    10621 agcggcggtg aagcccggcg gaaacggctg atccgggtca tctcggtttc gatcaccgtc
    10681 gcggtcctga ccatcggcat gaagctcgcc gcctggcttc tgaccggatc ggtcggcctg
    10741 ctttccgacg ccgccgagtc tgtggtcaac ctggtcgccg ccggggtcgc cctgatcgtg
    10801 atcctctggt caacccgccc ggccgacgag gaccacacct acggccacga gaaggccgac
    10861 tacctgtcgg ccgggctcga aggagccctg attctgctgg cggcggtgac catcgcctac
    10921 gccgcgatcg agcggctaat acacccggtc gagttgaact cggtcggaat cggcctggcg
    10981 atcagcgccg ccgcatcggt cctcaacctc ctcgccgcga ggctgctgat ccggaccggt
    11041 gaagaggaaa cctccctggt cctggtcgcc gatggccgtc acctgatggc cgacgtgatc
    11101 acctcggccg gggtgatcgt cggggtcgcg ctggtctggc tgaccggcct ggagcggctc
    11161 gatccggtca tcgcactcgt cgtcgccgcc aacatcatcc gcaccggaac gggcctggtt
    11221 tccgattcga tgtccgggct gatggacaag gccctgcctg aagacgaact ccagaaggtc
    11281 agccaggtgc ttgacgggtt cgagagtgac gacatccggt tccacgccct gagaacccgc
    11341 cgggccggcc gacggacttt catttcgatc cacgtgctgg tcccgggcaa ctggagcgtg
    11401 aagcagggtc acgacctgct cgaacgggtc gaagaggacc tgcgggcctg tttcgaacag
    11461 accacggtct tcacccacct tgaaccgatc gaggacccgg tctccttcgc cgacaccggc
    11521 ctcgaccgga cctccaaccc ccataccggg ggctaacgag ccggcgagcc tcgccccgcc
    11581 cgcggtcagg cttcgaaggc ggttcttgcc gcttcggggt cttcgaaggg tttccagctg
    11641 aggaccctgg cgccccggag ttcgaggagc caggcgagtt cgtcggtgta ggcgagctcg
    11701 tcgtcttcct cccagcgcca ctcccgtttc acgcccagca gtacccggtc cccggcaacc
    11761 cggcagttct cgatcaggac cgtctgctga actccgcccg gtttcttcgt tgcccaggcc
    11821 cgggcctcgt cgcggccctt gcgcagacct cgcatgccgt ggatttcgac ctcggggtcg
    11881 agcagctcga cgaactcatc gagctccgac cggttgaacg tcgccacgaa ctcgcggact
    11941 gtcttttctt caggagttac aggcctcacc atccaggtca tacagcgaga ccatcagttg
    12001 cctctaccgg gcgaaatgac ctggatgcat cattgcccgt ggcacttctt gtacttcttg
    12061 cccgagccgc accagcaggg atcgttgcgc ccgatgtctt cgcgttcggt cttgaccacg
    12121 gtcgccgagt tcgccggatc caccgcgccg cccacgttcg ggacgccacc gggaaccaca
    12181 cccggaaccc cgccggcgtc ggccggaccg ggagtcgcgc ccgcagcgac ctcggccagg
    12241 gctgaaggct gggcttccat ggtgccgccc gagtagtcgt aggtctgctc gtccggctcc
    12301 tcggccggcg gggcgaacga tccgttctga tccggttcga tttcgatctc gacgttgtag
    12361 atcaggcggc tgaactcctc ccagatgccg ttcatcagct cctggaacat cgtgtagccc
    12421 tcgttcttgt aggccaccag cgggtcgatc tgggcgaaac cgcgcaagtg aatgccttca
    12481 cgcaggtagt ccatgtcgta gaggtgctcg cgccagcggg agtcgatcac ctgcatcaac
    12541 agggtccgct cgaggtagcg catgatctct tcgccgaact cggcttcccg ttcgtcgtag
    12601 gccttctggg cgtcttcaca gaggtcctgc ttgagcgcct cgggttcggt cgattccggg
    12661 ggcaggccgg ccacgtcgat cgcgcaaggc cagatctggt ggagttgcga atcgaggcct
    12721 ggcaggtccc attcctcgat gatgtcgccg gccatgaact cgtcgaccag gcgtccgatc
    12781 accccgtcga tctcttcccg ggcgatctcc gacatgtcgc ggccttcgag aatctcgccg
    12841 cggtacttgt agatgacccg gcgctgctcg ttcatcacgt cgtcgtattc gaggacgcgc
    12901 ttgcggatca ggaagttctg ctcctcgacc ttcttctggg cgttctcgac ggtcttggtg
    12961 agcatcttcg cctcgagcgg catctcgtgg ccttcctcgt cggtcgggcc gagccggtcg
    13021 aggatcttgt agatgcggtc cccggcgaag aggcggatca cgtcatcctc ggccgagagg
    13081 aagaaccggg acgcgccggg gtcaccctga cggccggcac ggccgcgcag ctggttgtcg
    13141 atccgcctgg actcgtggcg ttcggtgccg cagatgaaca ggccgccgag ttccttgacc
    13201 tcttcggcct cggcgtcgca ttgcggtttc aactcggcgg cgatcttttc gaccgcctcg
    13261 ttgtagccct cgtcttccgg gttgagaccc tgcttggcaa cctgcggatg ggccatgccc
    13321 tccgggtctc cgccgagctt gatgtcaacg ccacggccgg ccatgttggt ggcgatcgtt
    13381 accgcgccct tgcgtccggc ctgggcgatt gtctcgcctt ccttttcggc gtgctcgggc
    13441 ttggcgttca ggaccacatg ctcgatcccc tgcttcttca gttcggccga gatcatttcc
    13501 gagacctcga ctgaaaccgt gccgaccagg atcggctggc cggtctcgtg gcgggcgacc
    13561 agttcgcgga tcacggcctg ccacttgcct tccttggtct tgaagatctg gtcgttctgg
    13621 tcgtcgcggg agatcgggcg gttggtcgga acctcgacca cggggacctt gtagatcttc
    13681 atgaactcga ccgcttcggt cagcgcggta ccggtcatgc ccgagagctt gtcgtagagg
    13741 cggaagtagt tctgcagggt gatcgtggcg agggtctggt tttcctcgcg gatcgccacg
    13801 ccttcctttg cttcgaccgc ctggtggagg ccttccgacc agcggcggcc ttcgagg
//
LOCUS       DAOQKN010000051        13764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_2091_length_13764_cov_5.975199, whole genome shotgun sequence.
ACCESSION   DAOQKN010000051 DAOQKN010000000
VERSION     DAOQKN010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 13764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13764
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2091_length_13764_cov_5.975199"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="PLJ64_05115"
     CDS             complement(<1..195)
                     /locus_tag="PLJ64_05115"
                     /inference="COORDINATES: protein motif:HMM:NF013777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HNC92898.1"
                     /translation="MSHRCNNCQPVPAEMRETAVVLERRWTMATIYACSSGATRFNEF
                     RQSLPEISPTTLSDRLEQLEQ"
     gene            complement(179..2473)
                     /gene="lon"
                     /locus_tag="PLJ64_05120"
     CDS             complement(179..2473)
                     /gene="lon"
                     /locus_tag="PLJ64_05120"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNC92899.1"
                     /translation="MDLLLVPLDDAIVFPSVTATLQIDVGDEEQVFLLPRREGEFARV
                     GVIADVVEKGQSPRGDTVATVVGLRRGLAGVAHSDENDPDALRIEVQEIHDGHPEDEH
                     TRELVREYRAIVEEILELRNADSRIVSFLRNVTEPGELADTSGLSPDLSFDQKFELLD
                     TLNVTARLEKAVEFQRERLAELQVRSKIRDHVESGAQQQQREYFLREQMKSIRKELGE
                     DEGDVIEEYEQKIAEAGMPEAVEEQAVKELRRLERQGEQSGEGSMIRSYLDWLIAVPW
                     SKRSDEKLDPAYTREVLDADHAGLDDVKRRITEFVAVRKLRKEREAEADSRADGAILT
                     LVGPPGTGKTSIGESVAKALGREFVRISLGGVHDEAEIRGHRRTYIGALPGRIVRALR
                     DAETMNPVILLDEVDKVGADWRGDPSAALLEVLDPAQNHSFRDHYLDVEIDLSEVVFL
                     ATANMLDTIPAPLLDRMEIIAFDGYTVEEKRAIARDYLLPRQVKRNGLTDDEVEVEDA
                     VLADLIAEYTREAGVRQLEREIGKLMRQAATRIASGEATAPVKVDEEFLRDALGKQKF
                     WQESVERTAIPGVSTGLAVTGTGGDVLFIEATAMDGDKGMVLTGQLGDVMKESARIAL
                     SYVRSHRDELGVEAEAFDREFHLHVPAGAIPKDGPSAGTAMTTALVSLLTGRPVKHTV
                     GMTGEVTLQGRVLPIGGLKQKVLAAHAAGLTEVILPFRNKADLDDVPGEVREAMSFHP
                     VSSVEEVIDLALEPAGSIADVAPM"
     gene            complement(2603..4015)
                     /locus_tag="PLJ64_05125"
     CDS             complement(2603..4015)
                     /locus_tag="PLJ64_05125"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNC92900.1"
                     /translation="MVPHLIFAEWYPFLGLDPFFEVLFLITFLIVVAMFFWTVVLFFR
                     GIKSDRHLARQEEVNPELFEWIFLVPALNEEVTIADSVSRLEAIEVARKRIVVINDGS
                     DDRTAEILAERTDPDLYVVERKAPQARQGKAAALNYAFDEVIKRFKLDPETTIFCIVD
                     ADGRIAPDSLSFVAKHFVDPKVGGVQTLVRIYNRHRILTWFQDIEFSIYGHLFQAGRN
                     KWGTAGMGGNGQYNRMAALQSIDDRDPGPAEEATVENDPEPEVAVEPASRGPWRDRLT
                     EDQDLGLRLMIAGWKCHHDNHATVEQQGVPGLRRLFRQRTRWSQGNLQAMGLIDEMVT
                     SRVHLPARFEQVVFLLMPVWQMIVGASLIASIYLFFFEGVRFIHNLEEWWWFYLLYLL
                     GFGGTVMGSIAAKIGDRSIILGIIKGILTGQVYAFYTWLLWPVLLRSSVRQVIGRGSW
                     AKTSREQITPADEPPASNPA"
     gene            complement(4000..5400)
                     /gene="wecB"
                     /locus_tag="PLJ64_05130"
     CDS             complement(4000..5400)
                     /gene="wecB"
                     /locus_tag="PLJ64_05130"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNC92901.1"
                     /translation="MTEQKGTDKPVPVKILVVVGTRPEAIKLVPMILALKENEMFIPQ
                     IVSTGQHQEMVKEVFDLAGIEIDFNLWVGGARNELNDRVREVMARLDDFVRVEYGADG
                     TVKQDEAAAGEYPMAIMVHGDTTSAFAAALAAFHLRIPVIHVEAGLRTQSNLTPFPEE
                     LNRQLITCIAAFNLAPTSHNLENLVRENVPVSQIFITGNTGIDSLQWAAGLEVEFSDP
                     IVAEICSSDRRVVTITAHRRENWGAGLDGIVAGVRLLAIEHPNTYFVVPQHPNPAVRQ
                     QLANLGDLPNVCLTDALSYPEFARLMARSYLIITDSGGIQEEAPSLGKPVLVCRESTE
                     RGEGVEAGTLRLVGTDPARIQAEADLLLKSEAAYQAMSEAENPYGDGRASERIVAALT
                     NLYVGGKMPEPFRSTYSRNAVIKASGYTLPGEASGRLDPALVPESERHHEEAVVDPVG
                     NLWAEFGTITEKWFRT"
     gene            complement(5410..6645)
                     /locus_tag="PLJ64_05135"
     CDS             complement(5410..6645)
                     /locus_tag="PLJ64_05135"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNC92902.1"
                     /translation="MATLVTELDLPGIDPADPELKGDRWHVAANGLLDSGKWLAQGPL
                     SLVVIDREAGEFFLRSRSAEFPGRLIADLFGIKDGPLRDQVDANIINRSGDDHRRLRS
                     LVNPALTPRAANRWRPVMREILEGLWEELETKEFDFVSRFSRPYPSQTIARVMGAPAV
                     DASRLHDWSEWIQRQFDPIALSDPETVETVQDKVAEFYAWVDPLIEERRGTPADDLIT
                     TLIRTEEEGEKLSDQELRNLVLNILVGGVDTTQSQLSQAIRLLAAAPDQWGLLREDPE
                     KMVPQAVSEVLRFEPVTAFTARQLVEEVEYRDVTFPAGTLLVVCSFTGNRDPEAFGDP
                     RDFDIAAERGKTRPLTFGAGIHYCVGSNIARAELEEGLRFLAEKVETIELNGLTELQA
                     VSGIYGIDRMDVRIQPVSG"
     gene            complement(6773..7495)
                     /locus_tag="PLJ64_05140"
     CDS             complement(6773..7495)
                     /locus_tag="PLJ64_05140"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC92903.1"
                     /translation="MNTNPSMNGAAGRILIADDEPSVRDSVGYALAQEGFEVTAAEDG
                     TDAEERLAGEFDFDLLILDIMMPGKSGLDICREVRSRSTVPIIMLTAKDAEVDKVVGL
                     EVGADDYVTKPFSLRELLGRVRAQLRRRELDRAPAPEQTRISAGPVTIDLARHVVTVN
                     GKTVSLTRSEFQLLRLLAARPGEVFSRAKIMEELWQTEFDGDERACDVHISNLRQKVE
                     TDPQRPELVLTVRGIGYKFADS"
     gene            complement(7492..9249)
                     /locus_tag="PLJ64_05145"
     CDS             complement(7492..9249)
                     /locus_tag="PLJ64_05145"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC92904.1"
                     /translation="MSGSPASSPRSSRGRRSRFPRRRHIGMRVWLAAGFTLVCFLTAG
                     ILYTFITDKSQDVLNERSTELAVGRTVRLADRLSEPDVDPKAELEAANGEGYTAWYFD
                     SDGDLVAPENPAATFPASKPYQEALDEALVGSRSSRDLKGGEIAIVGTTVGNEGEQVG
                     VVVGRYSRPVVIRSAIDTLRSESLRAALIAILIGTLVGVGIASVITGRLKRIAAKADD
                     LASGNFDVRLPVRGRDEIGDLARSLDSMRAALKESFGVLTADRDKLAAIFDGLGDAVM
                     VIDDDGSVRFHNSAANALLEQGGRAPEPMMSSVRRAAVEGFAAHPALRMGDRAYALQA
                     RSLPDEGAVLVVVRDRTDEMRKEFAERDFVSNAAHELRNPLAGISGAIEVLQSGAKDD
                     PPARDHFLNRLSADAERMSRLTQSLLTLARVEALGTGEVEVVDIGSAVKDVEAAVEVP
                     VGLELRTDVEPDLAAKGDPTLLRQVLIGLVTNACKNTPAPGSVTIRGRRVGDAEMLLE
                     VIDTGTGIPQAEIERVFERFYRRSETRRQEGFGLGLAIARRMADVMGGEMGANSVEGE
                     GSTFWVRLPLIDQSKTPIA"
     gene            9336..10052
                     /locus_tag="PLJ64_05150"
     CDS             9336..10052
                     /locus_tag="PLJ64_05150"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNC92905.1"
                     /translation="MRKLVFIPAWNEENSIEAVIADAREHVPDADVMVVDDGSTDRTA
                     ALARRAGALVASLPFNQGVGAAHQTAYIFALRHGYEICGQLDGDGQHPASEVTRLIAA
                     VEAGADLAVGSRFADDINLNIPEYKSTFARRIGQRLFSLLVSTSTRQTFTDTTSGMRA
                     LNERAMQLFADRYSSEFAEVESLQQAVRAGLVVREVPVHMLAREHGRSFIGPLTSAYY
                     IFKTLIVLLIGQLRPKQVEQ"
     gene            10049..10489
                     /locus_tag="PLJ64_05155"
     CDS             10049..10489
                     /locus_tag="PLJ64_05155"
                     /inference="COORDINATES: protein motif:HMM:NF021575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HNC92906.1"
                     /translation="MIWALAADTEPVVRLTPQARIIGAVLAIAFMALILDLIRRDKLL
                     ERYSIVWFLLGIGMLLGAIFPVALEWLAKAMGVRDVTIALFSVTLLILLALSLNFSVI
                     ASRQSKQITRLAQQQAIDGVEEEAASPAASSRGETPAPREQAPR"
     gene            complement(10428..11528)
                     /locus_tag="PLJ64_05160"
     CDS             complement(10428..11528)
                     /locus_tag="PLJ64_05160"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92907.1"
                     /translation="MITLEESTSHQPRVRAELEAALRQGPSHAYLFRGPSGSGKARAA
                     RAFAAGILSAGSADPEDSRRRALLDPSPHPDLVWLRPKGMSHAVQEIRDRIIHQAPLS
                     PFEGSARVFVIEAADALNEESQNAMLKTLEEPAPHAHLILLSAESEGLLPTIASRCQL
                     IEFGSLPFEVIEAELDGLASPEALHSAAKLAGGDLDKARFLAGKRGSDLRVAAERLMT
                     CALEDEFAGSPWLDLLAKARSAGEHSGELVQADFDEEKEEGIKHTKTEIEEAVRRAQR
                     KTRTGMLDLGLHLAAAWARDWATVIAGAPDLAFNQDRLEVLEAQAEGIELGQARDAVS
                     QIQETRRSFQLNVSEELALEALAFRLEKTLRD"
     gene            complement(11578..12171)
                     /gene="tmk"
                     /locus_tag="PLJ64_05165"
     CDS             complement(11578..12171)
                     /gene="tmk"
                     /locus_tag="PLJ64_05165"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNC92908.1"
                     /translation="MFITLEGVDGSGKTTQAAMLADALGASAMTLREPGGTDAAERIR
                     QLLADPETPLDPIAELMLFCAARADLVSRVIRPALEIGRTVICDRFTDSTIAYQGVAR
                     GLGIVRVRELSEVATDGLVPDLTLWLKVDPAFGLDRAGSDDRFEAEGVRFQERVAEAY
                     EDIARREPNRVVPIDATGSPEQVHRLILAEVEARLPA"
     gene            complement(12175..>13764)
                     /locus_tag="PLJ64_05170"
     CDS             complement(12175..>13764)
                     /locus_tag="PLJ64_05170"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HNC92909.1"
                     /translation="TDKFWDKAEADAALKGTVSPGVVKSVTSRKSSSKPPTPYNTNSF
                     QVDASSRLGITPKRAMDLAQDLYDDGFISYPRTDNTIYPDSLPLEKTIRSLVKIKDFA
                     AAAPILEKPLTPTQGKKFDAAHPPIYPTHALYPSALDGPKARVYELIVRRFLATFGEP
                     MVTESTRADIEAGSETYFVRGKVVLDPGYAGIYTYARSADEEIPALEEGQQLAIEGSP
                     WLVDKETQPPARLSEAKLVQLMDEQGLGTKATRPDIIQKLYNRRYVRNHPPEPTATGI
                     AMYEAFKEYVPDMATSAMTASLEGEMDEIAEEKMTKDEVVEDSRKLVHKTFKDLLQGE
                     EALSKMIWEGMDQDRILGPCIVCKEAGRTKEDGSPNVLRIIRAKKSGKSFVGCTGWEA
                     DNPESCDQTFPMPQPNFYEVTPLEENCSVCHRTPRVNVKTRGRAGRPWKLCFNDDCPT
                     MVEMREKKAERLAAQEAAKKAKELDEAEAKKSGKKKPAKKSGARKKSSKTKTVAARIA
                     NPPDLGTRRVKKAGARKPKKG"
BASE COUNT         2121 a         4539 c         4613 g         2491 t
ORIGIN      
        1 ctgctcgagt tgttcgagcc ggtcggaaag ggtggtcggt gagatctccg gcagcgactg
       61 gcggaactcg ttgaagcggg tggcgccgct ggaacaggcg tagatcgtcg ccatggtcca
      121 gcggcgctcg agcaccaccg cggtctcccg catctctgcg gggaccggct ggcagttgtt
      181 acatcggtgc gacatccgcg atgctcccgg ccggctcgag ggccaggtcg atcacttcct
      241 cgacgctgga gaccgggtgg aagctcatcg cttcccgaac ttccccgggc acgtcatcga
      301 ggtcggcctt gttgcggaag ggcaggatca cctcggtcag gcccgccgcg tgggcggcca
      361 gcaccttctg cttgagcccg ccgatcggca gtacccggcc ctggagtgtg acctcgccgg
      421 tcatgccgac agtgtgcttg accggtcggc cggtaagcag ggaaacgagg gctgtggtca
      481 tcgcggtacc ggcgctaggg ccgtccttgg gaatcgctcc ggccgggaca tgcaggtgga
      541 actcgcggtc gaaggcctcg gcctcgacgc caagctcgtc gcggtgcgag cgcacgtacg
      601 agagggcgat ccgcgccgac tccttcatca cgtcaccgag ctggccggtc aggaccatgc
      661 ccttgtcacc gtccatcgcg gtcgcctcga tgaagagcac gtcgccgccg gtcccggtca
      721 ctgcgaggcc ggtcgagact ccggggatcg cggtccgctc gaccgattcc tgccagaact
      781 tctgcttgcc gagcgcgtca cggaggaact cctcgtcgac cttgaccggg gctgtcgcct
      841 cgccggaagc gatccgcgtc gcggcctgcc gcatcagctt gccgatctcc cgttcgagct
      901 ggcgcacgcc ggcttcacgg gtgtactcgg cgatcaggtc ggccagcacc gcatcctcga
      961 cctcgacctc gtcgtcggtc aggccgtttc gcttgacctg ccggggcagg aggtaatcgc
     1021 gggcgatcgc ccgcttctcc tcgaccgtgt agccgtcgaa ggcgatgatc tccatgcggt
     1081 cgagcagtgg cgccgggatc gtgtcgagca tgttcgcggt ggccaggaag acgacctcgg
     1141 agaggtcgat ctcgacatcc aggtagtggt cccggaagct gtggttctgg gccgggtcga
     1201 gcacctcgag cagggcggcc gaggggtcgc cgcgccagtc ggcgccgacc ttgtcgacct
     1261 cgtccaggag gatcaccggg ttcatcgtct ccgcgtcacg cagggcacgg acgatccgtc
     1321 ccggcagggc gccgatgtag gtgcggcggt ggccgcggat ttcggcttcg tcgtgaactc
     1381 cgcccagcga gatccgcacg aactcgcggc cgagcgcctt ggcgaccgac tcgccgatcg
     1441 aggtcttgcc ggtaccgggc ggtccgacca gggtcaggat cgcgccgtcg gcccggctgt
     1501 cggcctcggc ctcgcgctcc ttgcggagct tccgcaccgc gacgaattcg gtgatccgcc
     1561 gcttcacgtc atcaagaccg gcgtggtcgg catcgagtac ctcgcgggtg taggccggat
     1621 cgagtttctc gtcggagcgt ttgctccacg ggaccgcgat cagccagtcg aggtaggaac
     1681 ggatcatcga gccctcgccc gattgctcgc cctgacgctc gagccggcgc agttccttca
     1741 cggcctgttc ctcaacggct tcaggcatcc ccgcttcggc gatcttctgc tcgtactcct
     1801 cgattacgtc gccttcgtcc tcgccgagct ccttgcggat cgacttcatc tgctcgcgca
     1861 ggaagtactc gcgctgctgc tgctgggcgc cggactcgac gtggtcgcgg atcttgctgc
     1921 ggacctgcag ctcggccagg cgctcgcgct ggaactcgac cgccttctcg aggcgggcgg
     1981 tcacgttgag cgtgtcgagc agctcgaact tctggtcgaa gctgaggtcg gggctgaggc
     2041 cggaggtgtc ggcgagctcg cccggctcgg tcacgttgcg cagaaagctg acgatgcggc
     2101 tgtcggcgtt tctgagttcg aggatctcct cgacgatcgc ccggtactcg cgaaccagtt
     2161 ccctggtgtg ctcgtcctcg gggtggccgt cgtggatctc ctgaacctcg atccggagcg
     2221 cgtccggatc attctcgtcg gaatgggcca caccggccag gccgcgacgc aggccaacca
     2281 ccgtggcaac ggtgtcgcca cggggggact gccccttctc gaccacgtcg gcgatcaccc
     2341 ctacccgggc gaactcgcct tcgcggcgcg gcagcagaaa gacctgttcc tcgtcaccga
     2401 cgtcgatctg gagcgtcgcc gtgaccgagg ggaaaactat tgcgtcatca agtggaacca
     2461 gcagaagatc cattagtaag tccaatttct gtagttagtc cttcgcacag tgtagcgcaa
     2521 gttgttgcgc tgtcaaccaa ctgcctcgga catcgggacg aacccgattc ccaccccgat
     2581 acaggaagcc tcgggtgagg ggtcaggccg ggttcgaggc cgggggttcg tcagcggggg
     2641 tgatctgctc ccgggaggtc ttggcccagg atccgcggcc gatcacctgc cggacgctgg
     2701 agcgcagcag gaccggccag agcagccagg tgtagaaggc atagacctgg ccggtgagga
     2761 ttcccttgat gatcccgagg atgatcgagc ggtcgccgat ctttgcggcg atcgacccca
     2821 tcacggtgcc gccaaaaccg agcaggtaga ggaggtagaa ccaccaccac tcctcgaggt
     2881 tgtggatgaa cctcacgcct tcgaagaaga agagatagat cgaggcgatc agggaagcgc
     2941 cgacgatcat ctgccagacc ggcatcagca ggaagaccac ctgttcgaaa cgggccggca
     3001 gatgaacccg ggaggtgacc atttcgtcaa tcaggcccat cgcctggaga ttcccctgtg
     3061 accagcgggt gcgctggcga aagagcctcc tcagaccggg cacgccctgc tgctcgaccg
     3121 tcgcatggtt gtcgtggtga catttccagc cggcgatcat caggcgaagt ccgaggtcct
     3181 gatcttcggt cagccggtca cgccagggcc cgcggctggc cggctcgacc gctacttccg
     3241 gctccggatc gttctcgacg gtcgcctcct cggccgggcc ggggtcacgg tcgtcgatcg
     3301 actgaagcgc cgccatccgg ttgtactgtc cgttcccgcc catcccggcg gtgccccact
     3361 tgttgcgccc ggcctggaag agatggccgt agatcgagaa ctcgatgtcc tggaaccagg
     3421 tcaggatccg gtggcggttg tagatccgga ccagggtctg aacgccccca accttagggt
     3481 cgacgaaatg cttcgcgacg aaggacaggc tgtccggggc gatgcggcca tccgcatcga
     3541 cgatgcagaa gatggtcgtc tccggatcga gcttgaaccg tttgatcacc tcgtcaaagg
     3601 cgtagttcag ggcggcggcc ttgccctgcc tcgcctgcgg ggccttgcgc tcgaccacgt
     3661 agaggtccgg gtcggtgcgt tcggcgagga tctccgcggt gcggtcgtcg gagccgtcgt
     3721 tgatcacgac gatccgcttg cgggccacct cgatcgcttc cagacggctg acgctgtcgg
     3781 cgatcgtcac ctcctcgttc agggccggga ccaggaagat ccactcgaac agctccgggt
     3841 tgacttcctc ctgccgggcc aggtggcgat cggacttgat cccccggaag aacaggacga
     3901 ccgtccagaa gaacatcgcg accacgatca ggaaggtgat caggaagagg acctcgaaga
     3961 acgggtccag ccccaggaag gggtaccact cggcaaagat caggtgcgga accacttctc
     4021 tgtgatcgtt ccgaactcgg cccagagatt gccgaccggg tccacgaccg cctcctcgtg
     4081 atgacgctcg gactccggca ccagcgcggg gtcgagccgg ccgctggcct cacccggcag
     4141 ggtgtagccc gacgccttga tcacggcgtt gcggctgtag gtggagcgga aaggttccgg
     4201 catctttcca ccgacgtaga gattggtaag cgccgcgacg atccgctccg aggcccgtcc
     4261 gtcgccgtac gggttctccg cttcgctcat cgcctggtag gcagcctcgc tcttcagcag
     4321 caggtcggct tcggcctgga tccttgccgg gtcggtcccg accaggcgaa gtgtgccggc
     4381 ctcgacgccc tccccgcgtt cggtcgattc gcggcagacc agcacaggct tgccgagcga
     4441 tggtgcctcc tcctgaatcc cgcccgagtc ggtgatgatc agataggagc gggccatcag
     4501 ccgggcgaac tcggggtagg aaagcgcgtc ggtcaggcag acgttgggca ggtcgccaag
     4561 gttcgccaac tgctgccgaa ccgccggatt cggatgctgc gggacgacga agtaggtgtt
     4621 cggatgctcg atcgcgagca gccggacacc ggcgacgata ccgtcgagcc cggcgcccca
     4681 gttctcgcgc cggtgggccg tgatggtgac gacgcgccgg tcactggagc agatttcggc
     4741 cacgatcgga tcggagaact cgacctcgag cccggccgcc cactgcagcg agtcgattcc
     4801 ggtgttgccg gtgatgaaga tctggctgac cggcacgttt tcccggacca ggttctcgag
     4861 gttgtgggag gtcggtgcga ggttgaaggc agcgatgcag gtgatcagct gccggttcag
     4921 ttcctccggg aacggggtca ggttcgactg ggtcctgagg cccgcttcga cgtggatcac
     4981 cgggatcctc aggtggaagg cggccagcgc ggcagcgaag gccgaggtcg tgtcgccgtg
     5041 gaccatgatc gccatcgggt attcaccggc tgcggcctcg tcctgtttga ccgtgccgtc
     5101 ggccccgtac tcgacccgga cgaaatcgtc cagccgggcc atcacctccc ggacccggtc
     5161 attcagttcg ttgcgcgccc cgccgaccca gaggttgaag tcgatctcga ttccggccag
     5221 atcgaagact tccttgacca tttcctggtg ctgaccggtg gaaacgatct gcgggatgaa
     5281 catctcgttc tccttgagag cgaggatcat cggaaccagc ttgatcgcct ccggccgggt
     5341 gccgacgacc acgaggatct tcaccggaac cggcttgtcg gttcccttct gctcggtcat
     5401 tggcgaagcc tacccggata caggctgaat ccgcacatcc atgcggtcta tgccataaat
     5461 cccgctgacc gcctgcagct cggtcaggcc gttcagttcg atcgtttcga ccttctccgc
     5521 gaggaaccgc aggccctcct cgagttcggc ccgggcgatg ttcgagccga cgcagtagtg
     5581 aatgccggcg ccgaaggtga gcggccgggt cttgccgcgc tccgcggcga tgtcaaagtc
     5641 ccgcggatcc ccgaaggcct ccgggtcccg gttcccggtg aaggaacaga ccaccagcag
     5701 ggtgccggcc gggaaggtca cgtcccggta ctcgacttcc tcaaccagct gacgggcggt
     5761 gaaggccgtg accggttcga aacgcagtac ctcgctgacc gcctgcggga ccatcttttc
     5821 cgggtcctcg cggagcagcc cccactgatc gggtgctgcg gccagcagcc ggatcgcctg
     5881 ggaaagctgg ctctgggtgg tgtcgacccc gccgaccaga atgttcagga ccaggttccg
     5941 gagttcctga tcggagagct tctccccttc ctcctcggtc ctgatcaggg tggtgatcag
     6001 gtcatcggcc ggggtgccgc gacgctcctc gatcagcgga tcgacccagg cgtagaactc
     6061 ggcgaccttg tcctgaaccg tctcgacggt ctccgggtcg gagagggcga tcgggtcgaa
     6121 ctgacgctgg atccattcgg accagtcgtg aagtcgggag gcatcgaccg ccggtgcgcc
     6181 catcacccgg gcgatggtct gggagggata gggccggctg aaacgcgaga cgaagtcgaa
     6241 ctccttggtt tccagttcct cccagagccc ttcgaggatc tcccgcatga ccggtcgcca
     6301 ccggttggcc gcccgcgggg tcagggcagg attgaccagg ctcctcagcc gccggtggtc
     6361 gtcaccgctc cggttgatga tgttggcgtc aacctggtcc ctgagtggcc cgtccttgat
     6421 cccgaacagg tcggcgatga gccggccggg gaattcggcc gaccgggaac gcagaaagaa
     6481 ctcccccgcc tcccggtcaa tgaccaccag cgaaagcggc ccctgcgcca gccacttgcc
     6541 cgagtccagc agtccgttcg ccgcaacatg ccaccggtcc cccttcaatt cgggatcagc
     6601 cggatcaatc ccgggcaggt ccagttcggt gaccagggtc gccattcccg aaggctatcc
     6661 cctgtagccg acccatccgc ttatggatgt cccgggtcgg cggggccgct gctcgaagca
     6721 ggtcacgcca accggcgcca gggttacagg aaggccgaag gctcgactgc ggtcaggaat
     6781 cggcgaactt gtagccgatt ccgcggactg tgaggaccag ttcggggcgc tgggggtcgg
     6841 tttcgacctt ctggcggagg ttggagatgt gaacgtcgca ggcgcgttcg tctccgtcga
     6901 attcggtctg ccagagctct tccatgatct tggcccggct gaacacttca ccgggacggg
     6961 cggcgagcag gcggagcagc tggaactcgg aacgggtcag gctgaccgtc ttgccgttga
     7021 cggtcaccac gtggcgggcc aggtcgatcg tgaccggtcc ggcggagatc cgggtctgct
     7081 cgggggcggg ggcgcgatca agctcgcggc gccggagctg ggcccggacc cggccgagca
     7141 gctcccgcag cgagaaaggc ttggtcacat agtcatcggc cccgacctcg aggccaacca
     7201 ccttgtcgac ctcggcgtcc ttggcggtga gcatgatgat cggcaccgtg gaacgggaac
     7261 ggacctcgcg gcagatgtcg aggccggact tgcccggcat catgatgtcg aggatcagca
     7321 ggtcgaagtc gaactcgccg gccaggcgct cttcggcatc ggtgccatcc tcggcggcgg
     7381 tcacctcgaa cccctcctgg gccagcgcgt agccgaccga gtcgcgaacc gaaggttcgt
     7441 cgtcggcgat caggatgcgc ccggcggcgc cgttcatgga tggattggtg ttcatgcgat
     7501 tggtgtcttc gactggtcta tcagaggaag gcgaacccaa aaggtactgc cctcgccctc
     7561 tacggaattg gctcccatct ctccgcccat cacatcggcc atcctgcggg cgatcgcgag
     7621 acccagtccg aagccctcct ggcgccttgt ttcggagcgc cggtagaagc gttcgaagac
     7681 cctttcgatc tccgcctggg ggatgccggt accggtgtca atcacctcga gcagcatctc
     7741 ggcgtccccg acccggcgac cgcggatggt cacagaaccg ggggccggag tgttcttgca
     7801 ggcgttggtg accagcccga tcagcacctg cctgagcagg gtcgggtcac ccttggcagc
     7861 cagatccggc tcgacgtcgg ttcgcagttc gagaccgacc gggacctcga ccgccgcttc
     7921 gacgtccttt accgcggagc cgatgtcaac cacctcgacc tcgccggtgc cgagcgcctc
     7981 gacccgcgca agggtgagca gcgactgggt cagcctggac atccgctcgg cgtcggcgga
     8041 gaggcggttc aggaagtgat cccgggccgg cgggtcatcc ttggccccgg actgaagcac
     8101 ctcgatcgcc cccgagatgc cggccagcgg attgcggagc tcatgggccg cgttgctgac
     8161 gaagtcgcgt tcggcgaact ccttgcgcat ctcgtcggta cggtcgcgga ccacgaccag
     8221 caccgcccct tcgtcgggaa gcgaacgggc ctggagcgcg taggcgcggt cacccatgcg
     8281 gagcgccggg tgcgcggcga aaccctcgac cgcggcccgt ctgaccgagg acatcatcgg
     8341 ttcgggggcc cggccaccct gttcgagcag ggcgttcgcg gccgagttgt ggaaccggac
     8401 cgagccgtcg tcgtcgatca ccatcaccgc gtcgccgagc ccgtcgaaaa tggcggccag
     8461 cttgtcgcga tcggcagtga gcacgccaaa gctttccttc agggcggccc gcatcgagtc
     8521 gagcgaacgg gccaggtcac cgatctcgtc gcggccccgg accggaagcc gcacatcgaa
     8581 gttgccggag gcgaggtcgt cggccttggc ggcgatccgc ttgagccggc cggtgatcac
     8641 cgaggcgatc ccgactccga ccagggtgcc gatcaggatc gcgatcaatg ccgcgcgcag
     8701 gctttccgag cgcagggtgt cgatcgccga gcggatcacc accggccgcg agtaacggcc
     8761 gaccacgacc ccgacctgct cgccttcgtt gccgaccgtc gttccgacga tcgcgatttc
     8821 gccgcccttc aggtcccggg aggagcgcga accgaccagc gcctcgtcga gagcctcctg
     8881 gtagggcttg ctcgcgggga aggtggcagc cgggttctcg ggggcaacca ggtcgccgtc
     8941 ggaatcgaaa taccaggcgg tgtaaccctc gccgttggcg gcttcgagtt cggccttggg
     9001 gtcgacgtcg ggctcgctga gccggtcggc cagacggacc gtgcggccga ccgcgagttc
     9061 ggtcgagcgt tcgttcagca cgtcctggct cttgtcggtg atgaaggtgt agagaatccc
     9121 ggcggtcagg aaacagacca gcgtgaatcc ggccgccagc cagacccgca tgccgatgtg
     9181 gcggcggcgc gggaagcgtg atcgcctgcc tcggctggat ctagggctgc tggcggggct
     9241 gccgctcatg agcggaccct agaggttgta gccaaactct gttcccggtg tggagactct
     9301 cggccacaac ctctatttca tgccagaatc gccgcgtgag gaaactcgtc ttcattccgg
     9361 cctggaacga ggaaaactcg atcgaagccg tgatagccga cgcccgggag catgttccgg
     9421 acgccgacgt gatggtcgtc gacgacggtt cgaccgaccg gaccgctgcc ctggcccgtc
     9481 gggccggtgc cctggtcgcc tcgcttccct tcaatcaggg ggttggcgcc gcccatcaga
     9541 cggcctacat tttcgccctg cggcacggct acgaaatctg cggccagctc gacggcgacg
     9601 gccagcatcc cgcctcggag gtcacccggc tgatcgccgc ggtcgaggcc ggagccgacc
     9661 tcgcggtggg ttcccgcttc gccgatgaca tcaacctgaa cattcccgaa tacaagtcga
     9721 ccttcgcccg gcggatcggc cagcggctgt tcagcctgct ggtctcgacc tcgacccggc
     9781 agaccttcac cgacacgacc agcggcatgc gggccctgaa cgaacgggcg atgcagctct
     9841 tcgccgaccg gtactcctcc gagttcgccg aggtcgagtc gcttcagcag gcggtgcgcg
     9901 ccggcctggt ggtgcgcgaa gtcccggtcc acatgctggc ccgcgagcac gggcgctcct
     9961 tcatcggtcc gctcacctcc gcctattaca tcttcaagac cctgatcgtg ctgctgatcg
    10021 gccagctccg accgaagcag gtggagcagt gatctgggcc ctcgcggccg ataccgagcc
    10081 ggtcgttcgg ctgacccccc aggcgcgcat catcggtgcg gtcctggcga tcgccttcat
    10141 ggccctgatc ctcgacctga tccgccgcga caagctgctc gagcgctact cgatcgtctg
    10201 gtttctgctc ggaatcggca tgctgctcgg cgcgatcttc cccgtcgccc tcgaatggct
    10261 ggccaaggcg atgggcgttc gcgatgtgac gatcgccctc ttctcggtca ccctgctgat
    10321 cctgctcgct ctctcgctca atttttcggt gatcgcttcc cgccagtcga agcagatcac
    10381 acggctcgcc cagcaacagg cgatcgacgg ggtcgaggaa gaagcggcta gtcccgcagc
    10441 gtcttctcga ggcgaaacgc cagcgcctcg agagcaagct cctcgctgac gttcagctgg
    10501 aagctgcggc gggtttcctg gatctggctg accgcgtcac gggcctggcc gagttcgatc
    10561 ccttccgcct gggcctcgag gacttcgagc cggtcctggt tgaaggcgag gtcgggggcg
    10621 ccggcgatga ccgtcgccca gtcacgggcc caggcggcgg cgaggtgaag gccgaggtcg
    10681 agcatgccgg tgcgggtctt ccgctgggcc cttcgcaccg cctcctcgat ctcggtcttg
    10741 gtgtgcttga tgccctcttc cttttcctcg tcgaagtcgg cctggacgag ctcgccggaa
    10801 tgctcgcccg ccgacctggc cttggccagc aggtcaagcc agggcgagcc ggcgaactcg
    10861 tcttcgaggg cgcaggtcat cagccgttcg gccgcgaccc tgaggtcgct gcctcgtttg
    10921 ccggccagaa accttgcctt gtcgaggtcc ccgccggcca gtttggcggc cgagtggagc
    10981 gcctcgggac ttgccagccc gtccagttcc gcttcaatca cctcgaacgg gagcgacccg
    11041 aactcgatca gctggcagcg ggaggcgatg gtcggcagca ggccttcgct ttcggctgaa
    11101 agcaggatca ggtgggcatg cggcgccggc tcttcgaggg tcttcagcat cgcgttctgg
    11161 ctttcctcgt tcagggcgtc ggccgcctcg atcacgaaga cccgggccga gccctcgaag
    11221 gggctgagcg gcgcctggtg gatgatccgg tcgcggatct cctggaccgc gtggctcatg
    11281 cctttcggcc gcagccagac caggtccgga tggggcgagg ggtcaagcag ggcgcgccgg
    11341 cgggagtcct cggggtcggc gctgccggcg ctcagtatcc cggccgcgaa agcccgggcg
    11401 gccctggcct tgccggaccc ggagggaccc cggaagagat aggcatgcga tggcccctgc
    11461 ctgagagcgg cttcgagctc ggcccggacc cggggctgat gggaggtgga ttcctccagc
    11521 gtgatcacgc cagcatcctc gcaagccgcg aggaggccac gccgcctccc cgccctttca
    11581 cgccgggagc ctggcttcga cctcggccag gatcagccgg tggacctgct cgggggatcc
    11641 ggtcgcgtcg atcggtacga cccggttcgg ctcgcgacgg gcgatgtctt cgtaggcctc
    11701 ggcgacccgt tcctggaaac ggaccccttc cgcctcgaag cggtcgtcgg acccggcccg
    11761 gtccagcccg aaggcggggt cgaccttcag ccagagggtg aggtcaggca ccaggccgtc
    11821 ggtcgccacc tcggagagct cgcgaacccg gactatcccg aggccgcggg ccaccccctg
    11881 gtaggcgatg gtcgagtcgg tgaagcgatc gcagatcacg gtccggccaa tctccagggc
    11941 aggccggatc acccggctga ccaggtcggc ccgggcggcg cagaagagca tcagttcggc
    12001 gatcgggtcg agcggggtct ccgggtcggc caggagctgc cggatccgct ccgcggcgtc
    12061 ggttccgccg ggctcgcgca gggtcatcgc gctcgctccg agagcgtcag ccagcatcgc
    12121 ggcctgggtg gtcttgcccg agccgtccac cccttcgagc gtgatgaaca cgcgtcagcc
    12181 ctttttgggc ttgcgagctc ccgccttctt cacccggcgg gtgccgaggt cgggcgggtt
    12241 ggcgatccgg gcggcgacgg ttttcgtctt cgaggatttc tttctggcgc cggacttctt
    12301 ggccggcttc ttcttgccgg acttcttcgc ttcagcttcg tccagttcct tcgccttctt
    12361 cgccgcttcc tgggccgcga gcctttcggc ctttttttcg cgcatctcca ccatggtcgg
    12421 gcagtcgtcg ttgaagcaga gcttccacgg ccggccggcc cggccccggg tcttgacgtt
    12481 gacccgcggg gtgcggtggc agaccgagca gttctcttcc agcggcgtca cttcgtagaa
    12541 gttcggctgc ggcatcggga aggtctggtc gcaggattcc gggttgtcgg cttcccagcc
    12601 ggtgcagccg acgaacgact tgccggactt cttggcccgg atgatccgca gcacgttcgg
    12661 cgagccatcc tccttggtcc gtccggcttc cttgcagacg atgcagggcc cgaggatccg
    12721 gtcctggtcc atgccttccc agatcatctt cgagagggcc tcttcaccct ggagcagatc
    12781 cttaaaggtc ttgtggacca gcttgcggct gtcctcgacg acctcgtcct tggtcatctt
    12841 ctcttcggcg atctcgtcca tctcgccttc cagcgaggcg gtcatcgcgg aggtcgccat
    12901 gtccggcacg tactccttga atgcctcgta catcgcgatg ccggtcgcgg tcggctccgg
    12961 cggatggttg cgcacgtatc gccggttgta gagcttctgg atgatgtcgg gccgggtcgc
    13021 cttggtgccg aggccctgtt cgtccatcag ctggaccagc ttcgcctccg agaggcgggc
    13081 gggcggctgg gtttccttgt cgaccaacca cggcgaaccc tcgatcgcga gctgctggcc
    13141 ctcttcgaga gccgggattt cctcgtcggc ggagcgggcg taggtgtaga tgccggcgta
    13201 accggggtcg agcacgacct tgccgcggac gaagtaggtc tcggagccgg cctcgatgtc
    13261 ggcccgggtc gattcggtca ccatcggctc gccaaaggtg gcgaggaagc gccggacgat
    13321 cagctcgtaa acccgcgcct tcggcccgtc gagagcactc ggatatagcg catgggtcgg
    13381 gtagatcggc gggtgggcgg cgtcgaactt cttgccctgg gtgggcgtga gcggcttttc
    13441 gaggatcggg gccgcggcgg cgaagtcctt gatcttgacc agcgagcgga tcgtcttctc
    13501 cagcggcagc gaatccgggt agatcgtgtt gtcggttcgc gggtaggaga tgaaaccgtc
    13561 gtcgtagagg tcctgggcga ggtccatcgc ccgcttcggg gtgatcccga ggcggctcga
    13621 cgcgtcgacc tggaacgagt tcgtgttgta gggggtcggc ggcttgctcg aggacttgcg
    13681 gctggtcacc gacttgacca cgcccgggct gaccgtgccc ttgagcgccg cgtcggcctc
    13741 ggccttgtcc cagaacttgt cggt
//
LOCUS       DAOQKN010000052        13010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_2242_length_13010_cov_6.092628, whole genome shotgun sequence.
ACCESSION   DAOQKN010000052 DAOQKN010000000
VERSION     DAOQKN010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 13010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13010
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2242_length_13010_cov_6.092628"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..1653)
                     /locus_tag="PLJ64_05175"
     CDS             complement(19..1653)
                     /locus_tag="PLJ64_05175"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HNC92910.1"
                     /translation="MGRASAASIPRAALDPGRPSFVVIQTDDQPLNEFDRRFRDLYDN
                     WRQIMPKTMALMKDKGITFSQYMTPFPLCAPSRATLLSGRYTQNHEVVRIGGPRGGWQ
                     AWRSNPIMFENLPVWLQRAGYRTMHFGKFMNNYGGIDAPAETVVPPGWDQWVSDATDN
                     STREFYGYRQNVNGTVTARLGWPYYDQQGGRDPEGCPWLGIEVCNYHTDSMSQQAEAA
                     IREAGDQPFYLQVDYHAPHGDSRPPIGPEPAPRHYDTALKTPGPRPRGFNERDITDKP
                     RFLQEADAAAPLTQTEISQLDTEYRKSIEALRSVDDGVGGIIKALRETGRLANTYVIY
                     TSDNGFFTGQHRISRGKLLPYEPALRVPFVIRGPGIRPGTRSFEPVANQDVAPTIIRL
                     AGARAALTIDGRSMIPFWKDPKKRSRRPILLSSYATITRLIPGDYPDDPEQPGDGGTE
                     DRTRRDKDASASVRSPDQNYVGIRLGPYKYIRYESGETELYVLSEDPAELENRAGDPR
                     YLKVINYLETQLADLRGCKGQSCRSWASPWPSPPGA"
     gene            complement(1751..3427)
                     /locus_tag="PLJ64_05180"
     CDS             complement(1751..3427)
                     /locus_tag="PLJ64_05180"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNC92911.1"
                     /translation="MGVLILFRNNRWRFRVLWLLTGLAAALLTAAFGHSVGLARARPN
                     LVVIQTDDQTVRMLHSYFVGPHGKKHRTMPHVQRLIEDRGLEFTNYYASIPVCSPSRS
                     ALLSGQYGHTSGLIRNVGKLGGVRGISRSPVWKRNLAVALRRVGYYTAHFGRFVNQYG
                     NPEGVDRRAVPPGWSWWATDWTPDVARRFYGYKLNVNGHIQGPFGKLHYQAWSNKDSR
                     KCPAGGLSCNYHTDQITTRALRVIRHAPRRPIYIQVDYEAPHATADGSGESEPASRHI
                     GSARRTPLPKPPGFNERNIRDKPRVLRSRARRLRAGQIAGMRTRWQRELESLRAVDES
                     VGRIVRTLRDTGRLARTYIFFIGDNGAFFGEHRYARSKFLAYEPSSNVPMLVRGPGVK
                     RGRRSGAIVSNVDLAPTIADLAGARLLLKPDGRSMKRLFRFPRRVGRRAVILESYRLP
                     SRPLYQHLEGEGIDTPHDLGPSASAVVPAVNYTAIRAGRYKYIDYEGGGRELYDLKLD
                     PYEVSNRVHWRGYRKVVRKLARQLKWRRFCSGAKCRRGTGRLPLPKLRRH"
     gene            3487..5625
                     /locus_tag="PLJ64_05185"
     CDS             3487..5625
                     /locus_tag="PLJ64_05185"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNC92912.1"
                     /translation="MSDREEKAAPLDPPSILMSGLHLAALWALAIVQPLLNLLGNNPD
                     FFVARDNTSSQIIIFVLLLTILPPLFAGLIEALVNLLSQAARWIVHLAFCAVLFWAIA
                     LQVLKQFADGPAWPMIIFAIVLGGLLTWAYAKARFMRSMTDILIIAPPVILIAFFFFS
                     DAKELTLSTTEVDAKTVNASNPHPVVMVIFDEFPAGSLMTPSGRINPKRFPNFAGLKG
                     TSTWYRNTATDASYTAIAVPSILTGQPADHDSLPTAADHPDSIFTLLGGSWQTHAIEP
                     ITQLCTEEICGEREGGNTGMADALSSLTTDLKAVSAHLLLPADMGSSLPDISQSFQGF
                     GGEEDSVERGRARQWVHDRLGEGENSLDGEADVDAFIRRLGSGGKSFDFVHVEKPHYP
                     WTHYPSGLKYSDGTEDFRSFIGETDWEGDGYITDRAHQAHLLEVGFTDNLLGRIISAL
                     KRDGRWDDTLFVATADHGAAWTKGAGRRSAEEETMGQIGMVPLFIKAPGQEQGKVVDR
                     PTCTTEIVPRMAQILGIDLPWTPATCDRKTVRIDNGSGKPVTLAFVKAIAQRQLYINR
                     MARLLGGDGADWDRAFRWGKRSDLIGQSTARFWRIGATNDAEASSETPSPRSFTFDSG
                     IELNPVLRQRGTISGLDEGTPLAVGVNGRIAAVGQSYEDNGQVRYSILLPQSTLREGS
                     NRIQLFEVTPFTGGAAGAASLRLLWSSTDQ"
     gene            5638..8010
                     /locus_tag="PLJ64_05190"
     CDS             5638..8010
                     /locus_tag="PLJ64_05190"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNC92913.1"
                     /translation="MTDVAKSGRESGNGGKAPFKRPSLILGAAHLAALWALTFAQPML
                     DLLGENPDFFVARGNSSGQIILYALVLTFAVPLVCLAIEALVRLASRDAQWYLHLFLV
                     GLFGACFVLQLIKNHLDWPAGILIAISIGLAVAGVFIYSRWRFPQAFMDVLSVAPIII
                     LVIFFGFSSTSRLVLPREQPDPVDVKIGTPAPVVMVVFDEFPVGSLMKPDGQIDGTRY
                     PAFAELAGHSTWYRNTSAAAAYTPLAVPAIFSGQKPNNDDLPIASDYPHSVFTLLGKT
                     YDLRVMEAATRICPDTLCPEEDNTAQDGSTSDLFSDLYVVSKYLLLPDSLTRNLPDVS
                     NSFSGFTESDQVDTNAESEVTGLDDGGETGATGTTGLTGATGATGDTQPERTGQGAAR
                     KLGRLFALESSADEFERVGEFNAKLKRGQQETLDLIHIEKPHYPWRHIPNGQRYSNLT
                     AEWSGLLPNDGPWMAPPRIVNIAMQRHLLEVGYTDTLLAQIIKRLKQTGLWERAMVVV
                     TADHGAAFQSKVPRRAATSENMGEVASVPLFIKSPGQVKPEVTDRRTCATDILPMIAD
                     QLGIEYPWPVAECDPERVTVVNSPNGEGFTSPAKMLRQRQKLLINRIQSVFGTGHGWG
                     PYYRFGPHRELIGKKVKNLEVLPLKRWRALPDERNAAKNYDPTAPTLRGLLQRGVTDR
                     IPEGSVLAVAVDGKIQAVGWTFKDGTGRGPGFSILLPPESLRAGFNRIAIYLLRDNGK
                     KLRLVYDGSRPLAPELVEAQNEARAEYRREMGLPALPDPLSTGGQNGDGS"
     gene            8000..8491
                     /locus_tag="PLJ64_05195"
     CDS             8000..8491
                     /locus_tag="PLJ64_05195"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC92914.1"
                     /translation="MGPETPLRIAGPADIGPVTRLIAGFRDFLADDSPTNAEIEATVG
                     VLLRDRSTEFLLVGDPEAGFVQTRFRLSVWTGNEDAWLEDLFVDESARGKGYGRTLVE
                     AAVERARSRGCDRIQLDVNQSNEPAVKLYESCGFRPVHNPEKWGSSPDFFYTREIQRQ
                     QVG"
     gene            complement(8441..>9009)
                     /locus_tag="PLJ64_05200"
     CDS             complement(8441..>9009)
                     /locus_tag="PLJ64_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92915.1"
                     /translation="EVEAAGFRRVLFGPDPGRLAAEFEGRSSLWELGLEATTGQVIWN
                     ENRARLFPDPGPGRVPLIWSRSIGEGELRTLPPSAGKPGFYDTLDGGRPQLLGPAIVV
                     NRVVGAVGRGELRAALVPEGMEFLAENHVNVIRSRGPAVDWEGVLAALRMPGVVERVR
                     LLTGNTQISARELTHLLPLDLAGVEEVG"
     assembly_gap    9010..9109
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9110..9954)
                     /locus_tag="PLJ64_05205"
     CDS             complement(<9110..9954)
                     /locus_tag="PLJ64_05205"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNC92916.1"
                     /translation="MSEPPKPRPSGRCGDLSPVSLQWLEQADPVSRKARGQYLTPRPV
                     AEALIDRVGLRPGLRVLDPGVGTGELLRALLDREPDLEVTGWDTDPTAIRAAGELVPE
                     ARLKLRSALDPDAGSSTPAGSSGPDGTETGGFDLVIGNPPYFQLRLDPEERKRFAGVI
                     SGRPNIFALFFQIGLQELKPGGTLAFIVPPSMNSGAYFEALREYIAARAKITDLTILE
                     GTGLFEGANTAIQLLVLRKNGPEEPVGRISGTGDGNPTHRGFIYRREVEAAGFRRVLF
                     GPDPGR"
     gene            10063..11835
                     /locus_tag="PLJ64_05210"
     CDS             10063..11835
                     /locus_tag="PLJ64_05210"
                     /inference="COORDINATES: protein motif:HMM:NF017722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfotransferase family protein"
                     /protein_id="HNC92917.1"
                     /translation="MSRLATTAAGLFILVLGVAVTSAAEAKEIAAYPSPNVRVASDTT
                     TISFRGIKPKALGRIIVRGSRSGGHKGRLRVHSDGRGVSFIPRKKFDPKEKVTVISKR
                     NTFFGTGKRSKHRYSFVTGLLQPKGWRDEPQVPPQGERPPEWTTYKSFPLLVPKITIN
                     TSKPGVANGDIFMAPRTNGPMIVDNDGGLVYYRPGQRVTDFRTQTFRGKPVLTWWRRA
                     QVGKHVESNYAIAGTDYKVIKRFEAGNGYTSDPHEFTMASDTTAYVNSFRNVIMDLSK
                     YGGLKRTPVMDNVAQEVDLLTGQVIWEWHSLGNIRVPETYKDIPSKITRPFDYFHLNS
                     ISRDSDGNMLISARHTCALYKINRETGKVMWRMGGKDSDFKLASGFRFCYQHDFRPHP
                     NGTYSIFENGAADGELKPKARMSKAYIFRINQKKKTTALVKTYIHPGNLLSPSQGNTQ
                     LLPNGNVFTGWGSLEYCTEFAPDGEVLWDMRYSAAAVTYRCYRSPWTGTPVPGAIGVK
                     SETDGPNSRVWVSWNGDTQVRTWRVLAGNPGNLAYSTEVPRDGFETSIPVTGQPAAFR
                     LVGLDGQGKVLGRSRVNKLGELTR"
     gene            11832..>13010
                     /locus_tag="PLJ64_05215"
     CDS             11832..>13010
                     /locus_tag="PLJ64_05215"
                     /inference="COORDINATES: protein motif:HMM:NF017722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfotransferase family protein"
                     /protein_id="HNC92918.1"
                     /translation="MNLALANRNRRAAGSAAAALATALAVVVAALALAAPAGAKPIAA
                     YPSPGSVYASPTTNIALSGVTRASVGRIMVRGSRSGFHRGRIEAWAGPVGVSFIPSRP
                     FAPLEKVTVTSRSHPFYGTGGSRSYSFKTGEFLPENLGADPFSPAKGQTPRASQTYKT
                     LRLKVPKIVVHANEPGKSNGKIFYAPRTSGPTILDADGNLVWYRPGLRITDFRAQVYN
                     GHRILTWWRRDTFGKRVTSRFEMANRHYKVFRRFGGGNGFTGDPHEFNLTSRGTAFVT
                     AYKTAVVDLSRFGGPRRAFLLDYIGQEIDIKTGLVVWEWHPLGNLPMNRTYLPIPRRN
                     TRPFDWFHMNSINDDNDGNVLISARHTQALYKINRKTGRIMWQIGGKGGDFKLGKGVR
                     F"
BASE COUNT         2236 a         4426 c         4125 g         2123 t
ORIGIN      
        1 agactcgccc gactgcagtt aagcccccgg aggggaaggc cacggactgg cccagctccg
       61 gcagctctgg cctttgcagc cgcggagatc ggcgagctgg gtttcgaggt agttgatgac
      121 cttcaggtag cgggggtcgc cggcacggtt ttccagttcg gccgggtcct cgctgagtac
      181 gtacagttcg gtttcgccgc tctcgtaacg gatgtacttg taggggccga gccggatccc
      241 gacgtagttc tggtccgggc tgcggaccga ggcgctcgcg tccttgtcgc gcctggtgcg
      301 atcctccgtt cctccatcgc cgggctgctc cggatcatcc gggtaatcgc cggggatcag
      361 gcgagtgatc gtcgcgtagc tggaaagcag gatcggccgg cgtgaccgct tcttcgggtc
      421 cttccagaac ggaatcatcg agcggccgtc gatcgtcagg gcggcccggg cgccggccag
      481 cctgatgatg gtcggggcga catcctggtt ggccaccggc tcgaagctcc tggtccccgg
      541 cctgattccc gggccgcgga tcacgaacgg cacccgcagg gccggctcgt agggcagcag
      601 cttgccgcgg ctgatccggt gctggccggt gaagaagccg ttgtccgagg tgtagatcac
      661 gtaggtgttg gccaggcggc cggtctcccg caatgccttg atgattcccc cgaccccgtc
      721 atcgaccgaa cgcagggcct cgatcgactt gcggtactcg gtgtcgagct ggctgatctc
      781 ggtctgggtg agcggtgcgg ccgcgtcggc ttcctggagg aaccgcggct tgtcggtgat
      841 gtcccgctcg ttgaagccgc gcgggcgtgg tccgggtgtc ttcagggcgg tgtcgtagtg
      901 acggggcgcc ggttccgggc cgatcggtgg ccgtgaatcg ccatgcgggg cgtggtagtc
      961 gacctggagg tagaagggct ggtcaccggc ctcccggatc gccgcttctg cctgctggga
     1021 catcgagtcc gtgtggtagt tgcagacctc gatccccagc caggggcagc cctcggggtc
     1081 gcggccgccc tgctggtcgt agtagggcca gcccaggcgt gccgtgacgg tgccgttgac
     1141 gttctgccgg tagccgtaga actcacgggt cgagttgtcg gtcgcatcgg agacccactg
     1201 gtcccacccg ggcggaacga ccgtctccgc gggggcatcg atgccgccgt agttgttcat
     1261 gaacttgccg aaatgcatgg tccggtagcc ggcccgctga agccagaccg gcaggttctc
     1321 gaacatgatc ggattgctcc gccaggcctg ccacccgcca cgcggcccgc cgatccggac
     1381 tacctcgtgg ttctgggtgt aacgcccgct gagcagggtt gcccgtgaag gtgcgcagag
     1441 cgggaagggc gtcatgtact ggctgaaggt gatgcccttg tccttcatca gggccatcgt
     1501 cttcggcatg atctggcgcc agttgtcgta aaggtcccgg aagcggcggt cgaactcgtt
     1561 caggggctgg tcatcggtct ggatcacgac gaaactaggc cggcccggat caagggcggc
     1621 ccggggaatc gaagccgcgc tggcgcggcc catccccacc gccagaagca gtgcgaagag
     1681 cagcgtgaat gctgccgacc gggtgaagat gttgcgattt gctcccctga tgtctgcccc
     1741 ctgaaaccgg tcagtggcgc cggagtttcg gcaatggaag ccgcccggtt ccgcgcctgc
     1801 attttgcccc ggagcagaac ctacgccatt tcaactgccg ggccagtttc cggaccacct
     1861 tccggtaccc gcgccagtgg acacgattgg aaacctcgta cgggtcgagc ttgaggtcat
     1921 agagctcgcg cccgccgcct tcgtaatcga tgtacttgta acggccggcg cggatcgccg
     1981 tgtagttgac cgcggggacc acggccgagg ccgagggacc aaggtcatgg ggcgtgtcga
     2041 tcccttcgcc ttccagatgt tggtagagcg gtctcgaagg cagccggtag gactcgagga
     2101 tcacggcccg ccgtccgacc cgccggggga accggaacag ccgcttcatt gaccttccgt
     2161 ccggcttcag cagcagccgg gcgccggcga gatcggcgat ggtcggcgcg agatcgacgt
     2221 tgctgacgat cgcgcccgag cgacggcctc gcttgacccc gggaccccga accagcatcg
     2281 gcacgttgct cgaaggctcg taggcgagga acttggaccg ggcgtagcgg tgctcgccga
     2341 agaaggcgcc gttgtcaccg atgaagaaga tgtaggtgcg ggccaggcga ccggtgtccc
     2401 gaagggtcct gactatcctt ccgacgctct cgtccacggc ccggagcgac tcgagctcac
     2461 gctgccagcg ggtccgcatc ccggcaatct ggccggccct caggcgccgg gcccgggaac
     2521 gcaggacccg gggcttgtcg cgaatgttcc gctcgttgaa gcccggaggc ttcggcagcg
     2581 gcgtgcggcg ggccgaaccg atgtgacggc tggcgggctc cgactcgcca ctgccgtcgg
     2641 cggtcgcatg cggggcctcg taatcgacct ggatatagat cggccgccgc ggcgcgtgcc
     2701 ggatcacccg cagggcccgg gtcgtgatct gatccgtgtg gtagttgcag ctcagcccgc
     2761 cggccgggca tttgcggctg tccttgttgc tccaggcctg gtagtggagc tttccgaacg
     2821 gcccctggat gtgaccgttc acgttcagct tgtacccgta gaaccggcgg gcgacatccg
     2881 gagtccagtc ggtcgcccac caggaccatc ccggcgggac ggcacgccgg tcgacgccct
     2941 ccgggttgcc gtactggttc acgaaccggc cgaaatgcgc cgtgtagtaa ccgacccggc
     3001 gcagggcgac ggccaggttc cgcttccaga ccgggcttcg cgaaatgccc cttaccccgc
     3061 ccagcttgcc gacgttgcgg atcagcccgc tggtgtgccc gtactgcccg gaaagcaggg
     3121 ccgaacggga aggcgaacag accggaatcg acgcgtagta gttcgtgaac tcaaggccgc
     3181 ggtcctcgat caggcgctgg acatgcggca tcgtgcggtg cttcttgcca tgcggaccga
     3241 cgaagtacga gtggagcatc cgcacggtct ggtcgtcggt ctggatgacc accaggttgg
     3301 gacgggcccg ggcaagcccg accgaatggc cgaacgcagc ggttagcagt gccgcagcca
     3361 ggccggtcag cagccagagg acgcgaaaac gccagcggtt gttcctgaag aggatcaaga
     3421 cgcccatcgg ttgaaacccg cttcatagcg gcgagtttcc cttcaagaga ataggatcgc
     3481 cgaccgatgt ctgaccggga ggaaaaggcc gcacccctgg atccgccctc gatcctgatg
     3541 agcggcctcc acctcgccgc cctctgggcg ctggcgatcg tccagccgtt gctgaacctg
     3601 ctgggcaaca accccgactt cttcgtcgcc cgcgacaaca ccagcagcca gatcatcatc
     3661 ttcgtcctgc tgctgacgat cctgccgcca cttttcgcgg ggctgatcga ggccctggtc
     3721 aatctgctca gccaggcggc ccggtggatc gttcacctcg ccttctgtgc cgtgctcttc
     3781 tgggcgatcg ccctgcaggt gctgaagcag ttcgcggacg gtcctgcctg gccgatgatc
     3841 atcttcgcga tcgtgctcgg cggccttttg acctgggcct acgccaaggc ccgcttcatg
     3901 cgctcgatga ccgacatcct gatcatcgcc ccgccggtga tcctgatcgc cttctttttc
     3961 ttcagcgacg ccaaagagct cacgctctcg accacggagg ttgacgccaa gacggtgaac
     4021 gcctcgaacc cgcatccggt cgtgatggtc atcttcgacg agttcccggc cggctcgctg
     4081 atgacccctt cgggccggat caacccgaag cgcttcccca acttcgccgg cctgaagggc
     4141 acctctacct ggtaccggaa cacggcgacc gacgcttcgt acacggcgat cgcggtgccc
     4201 tcgatcctga ccggccagcc cgcggatcac gacagcctgc cgaccgcggc cgaccacccc
     4261 gattcgatct tcaccctgct gggcgggagc tggcagaccc acgcgatcga accgatcacc
     4321 cagctctgca ccgaggagat ctgcggcgag cgggaaggcg gaaacacggg catggccgat
     4381 gcgctctcct ccctgaccac tgacctgaaa gcggtttccg cccatctgct gctgccggcc
     4441 gacatgggtt cctccctgcc ggacatttcc cagagcttcc agggtttcgg tggcgaggag
     4501 gactcggtag agcggggccg ggcccgccag tgggtgcatg accgcctcgg cgagggcgag
     4561 aactcgctgg atggtgaagc tgacgttgac gcgttcatcc gccggctggg aagcggcggc
     4621 aagagcttcg acttcgttca cgtcgagaag ccgcactatc cctggaccca ctacccctcg
     4681 gggctgaagt actccgacgg gaccgaggac ttccgcagct tcatcggtga aaccgactgg
     4741 gagggtgacg gctacatcac ggaccgggcc catcaggccc atctgctcga ggtcggcttc
     4801 accgacaacc tgctcggccg gatcatttcc gcgctgaaga gggacggccg ctgggatgac
     4861 acgctgttcg ttgccacggc cgatcacggt gcagcctgga ccaaaggtgc cggccgacgc
     4921 tcggcggagg aagaaacgat ggggcagatc gggatggtcc cgctcttcat caaggcaccc
     4981 ggccaggagc agggcaaggt agtcgaccga ccgacctgca ccacggaaat cgtcccccgg
     5041 atggcgcaga tcctcggcat cgacctgccc tggacgccgg ccacctgcga ccgcaagacc
     5101 gtccggatcg acaacggaag cggcaagccg gtgactctcg ccttcgtcaa ggcgatcgcc
     5161 cagcgccagc tctacatcaa ccggatggcc cggctgctcg gcggcgacgg agccgactgg
     5221 gaccgcgcct tcaggtgggg caaacggagc gacctgatcg gccagtccac ggcccggttc
     5281 tggaggatcg gcgccacgaa cgacgccgaa gcgagctccg aaactccctc gccccgctcg
     5341 tttacgttcg attcggggat cgaactgaac ccggtcctga ggcaacgagg cacgatttcc
     5401 ggactggacg aaggcacgcc gctggcggtt ggcgtcaacg gaaggatcgc cgcggtcggc
     5461 cagtcctacg aagacaacgg ccaggtcagg tactcgatcc tgctgccgca atcaacgctc
     5521 cgggaagggt ccaaccggat tcagttgttc gaagtgactc cattcacggg aggtgccgcc
     5581 ggagcagcct ccttgaggct tctctggtcc tcgaccgacc aatagactgg ccagtccgtg
     5641 actgatgtcg ccaagtccgg gcgcgagtcc ggaaacggag gtaaagcgcc gttcaagcgt
     5701 ccgtccctga ttctgggggc ggcccatctg gctgcgctct gggcgctcac cttcgcccag
     5761 ccgatgctcg acctgctcgg cgagaacccg gacttcttcg tcgcccgggg caacagctcc
     5821 ggccagatca tcctttacgc cctggtcctc acctttgcgg tgccgctggt ttgcctcgcg
     5881 atcgaggcgc tggtccgcct ggccagtcgc gacgcccagt ggtatctcca ccttttcctg
     5941 gtcggactgt tcggggcctg cttcgtcctg cagctgatca agaaccacct cgactggccg
     6001 gccgggatcc tgatcgcgat ttcgatcggc ctggccgtgg ccggggtctt catctattcc
     6061 cgctggcgct ttccccaggc cttcatggac gtgctcagtg tcgccccgat catcatcctg
     6121 gtgatcttct tcggtttcag cagcacctcg cgcctggtcc tgccccggga gcagccggac
     6181 cccgttgacg tgaagatcgg aactccggcc ccggtcgtga tggtcgtctt cgacgagttc
     6241 ccggtcggct cgctgatgaa gcccgacggt cagatcgacg gaaccagata cccggccttc
     6301 gccgagctgg ccggccattc gacctggtac cggaacacca gcgccgccgc tgcctacacc
     6361 ccgcttgccg tgccggcgat cttctccggg cagaagccga acaacgacga cctgccgatc
     6421 gcctccgact atccgcacag cgtcttcacc ctgctcggca agacctatga cctgagagtg
     6481 atggaggccg ccacgcggat ctgccccgac acgctctgtc cggaagagga caacaccgcg
     6541 caggacggct cgacctccga cctcttctcg gacctttacg tcgtctccaa gtacctgctg
     6601 ctgccggatt cgctgacccg gaacctcccc gacgtctcca actccttctc gggcttcacc
     6661 gaatcggatc aggtcgacac caacgccgaa tccgaggtga ccggtctcga tgacggcggt
     6721 gaaaccgggg caaccggcac caccggactg accggagcga cgggcgctac cggagacacc
     6781 cagccggagc ggaccgggca gggtgccgcc cgcaagctcg gccggctgtt cgcgctcgaa
     6841 tcctcggccg acgaattcga gcgggtcggc gagttcaacg cgaagttgaa gcggggccag
     6901 caggagaccc tcgacctgat tcacatcgaa aagccccact acccctggcg ccacatcccg
     6961 aacggccagc gctactcgaa cctgaccgcg gaatggtcgg gcctgctgcc caatgacggt
     7021 ccctggatgg ccccgccgcg gatcgtcaac atcgccatgc agcggcacct gctcgaggtc
     7081 ggatacaccg acaccctgct cgcccagatc atcaagcggc tgaagcagac cggcctctgg
     7141 gagagagcaa tggtcgtcgt caccgccgac catggagccg ccttccagtc gaaggtcccg
     7201 cgccgggcgg cgaccagcga gaacatgggg gaggtcgcgt cggtcccgct cttcatcaag
     7261 tcacccgggc aggtcaaacc ggaagtgacc gaccggcgca cctgcgcgac cgacatcctg
     7321 ccgatgatcg ccgaccagct cgggatcgag tacccgtggc ccgtcgccga atgtgacccg
     7381 gagcgggtca ccgtggtcaa ttcgccgaac ggggagggtt tcacctcccc cgcgaagatg
     7441 ctgcggcagc ggcagaaact gctgatcaac cggatccaga gcgtcttcgg gaccggtcac
     7501 ggctggggcc cctactaccg cttcgggccc cacagggaac tgatcggcaa gaaggtgaag
     7561 aacctcgagg tgctgccact gaagcgctgg cgcgccctgc cggatgaacg gaacgcagcc
     7621 aagaactacg acccgaccgc ccccaccctg cgcggcctgc tccagcgcgg ggtcaccgac
     7681 cggatcccgg aagggagtgt gctggccgtg gcagtggacg ggaagatcca ggcggtcggc
     7741 tggaccttca aggacggcac cggccgcggg cccgggttct cgatcctgct tcctcccgag
     7801 tcgctcaggg ccggcttcaa ccggatcgcc atctacctgc tccgggacaa cgggaaaaag
     7861 ctccgcctgg tctatgacgg ttccaggccg ctggcacccg agctggttga agcgcagaac
     7921 gaggctcggg ccgagtaccg cagggagatg ggactgcccg ccctgccgga cccgctttcg
     7981 accggaggcc agaacggtga tgggtcctga gaccccgctc agaatcgccg ggccggccga
     8041 tatcggaccg gtcacccgcc tgatcgccgg tttccgggat ttcctcgcgg acgactcgcc
     8101 gaccaacgcc gagatcgaag cgacggtcgg ggtccttttg cgggaccgct cgaccgagtt
     8161 cttgctggtc ggcgaccccg aggccgggtt cgtgcagacc cgtttccggc tttcggtctg
     8221 gaccggcaac gaggacgcct ggctggaaga cctcttcgtt gacgagtcgg ctcgcgggaa
     8281 gggttacggg cgcaccctgg tcgaggcggc ggtcgagcgg gccaggtcgc gcggttgcga
     8341 ccggatccag ctcgacgtca accagtccaa cgagccggcg gtgaagctct acgaatcctg
     8401 cggattccgg ccagtccaca atccggagaa gtggggaagt tcacccgact tcttctacac
     8461 ccgcgagatc cagcggcagc aggtgggtta gttcccgggc cgagatctgg gtgttcccag
     8521 tcagcagccg gaccctttcg accactcccg gcatccgcaa cgcggcgagg acgccttccc
     8581 agtcaaccgc cgggccacgg ctgcggatca cgttgacgtg gttctccgcc aggaactcca
     8641 tcccctccgg aacgagcgcc gcccgcagct ccccccggcc gaccgccccg accacccggt
     8701 tgaccacgat cgccgggccg agaagctgcg gccggccacc atcgagcgtg tcgtagaagc
     8761 ccggtttccc ggcgctcggg ggaagggtcc ggagttcccc ttcgccgatg ctgcgggacc
     8821 agatcagcgg aacccggccc ggaccggggt ccgggaacag ccgggcccgg ttctcgttcc
     8881 agatcacctg gccggtggtg gcttcgaggc caagctccca gagcgagctt cgcccctcga
     8941 actcggccgc cagccggccc gggtcgggac cgaacagaac ccgccggaaa cccgccgcct
     9001 caacctcccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ggcccgggtc
     9121 gggaccgaac agaacccgcc ggaaacccgc cgcctcaacc tcccgccggt agatgaaccc
     9181 ccggtgggtc ggatttccgt cacctgtacc ggaaatccga cccaccggtt cttcgggacc
     9241 gttctttctc aggaccagga gctggatcgc ggtgttggct ccttcgaaga ggccggttcc
     9301 ctcgaggatg gtcaggtccg tgatcttcgc ccgggccgcg atgtattcgc gcagggcctc
     9361 gaaataggcg ccgctgttca ttgacggcgg gacgatgaag gcgagcgtgc cccccggttt
     9421 cagctcctga aggccgatct gaaagaagag cgcgaagatg ttgggccggc cggagatcac
     9481 cccggcgaaa cgcttcctct cctctgggtc gaggcgaagc tggaaatagg gcgggttccc
     9541 gatcaccagg tcaaagccac cggtttcggt cccgtccggg ccggagctcc ctgccggggt
     9601 ggagctgcct gcgtctggat cgagcgcgga gcggagcttc agacgcgcct cgggaaccag
     9661 ttcgcctgcg gcgcggatcg cggtcggatc ggtgtcccag ccggtcacct cgaggtccgg
     9721 ttcccggtcc agcagggccc ggagcagctc gccggtgccg acccccggat cgaggactct
     9781 cagacccggt cgaagcccga cccggtcgat cagcgcctcg gcgaccggcc gcggggtcaa
     9841 atactggccc cgggccttgc gggaaaccgg atcggcctgt tcgagccact gaaggctgac
     9901 cggggagagg tcgccgcaac ggcctgaggg gcgcggtttg ggcggctcac tcaaagcggc
     9961 gccatcgtat ctgcctgaag agtggccgct ccacccgcaa cttccggcgc tttgagtggt
    10021 ctaagattgc ggatccgcca atcacgatga gaaagggagc aggtgtcgcg cttagccacg
    10081 accgcagcag gcctgttcat tcttgtactt ggtgtagccg tcacttcggc tgccgaggca
    10141 aaggaaatcg ccgcataccc cagtccgaac gtccgggtcg cttcggatac gaccaccatc
    10201 tcgttccgcg gcatcaaacc gaaggcgctt ggcaggatca tcgtgcgcgg ctcccgcagc
    10261 ggcggccaca agggccggct tcgcgtccat tccgacggcc ggggcgtcag cttcatcccc
    10321 cggaagaagt tcgacccgaa ggaaaaggtg accgtgatct cgaagcggaa caccttcttc
    10381 ggcaccggca agcgcagcaa gcaccgctac agctttgtga ccggcctgct ccagcccaag
    10441 ggctggcggg atgagccgca ggttccgccg cagggcgagc ggccgcccga gtggaccacc
    10501 tacaagtcct tcccgctgct ggtcccgaaa atcacgatca acaccagcaa acccggcgtg
    10561 gccaacggtg acatcttcat ggcgccccgc accaacggtc cgatgatcgt cgacaacgac
    10621 ggcggcctgg tctactaccg gcccggccag cgggtcaccg acttccgcac ccagaccttc
    10681 aggggcaagc ccgtcctgac ctggtggcga cgcgcccagg tcggcaagca cgtcgagagc
    10741 aactacgcga tcgccggtac cgactacaag gtgatcaagc gcttcgaagc cggcaacggc
    10801 tacaccagcg acccgcatga gttcaccatg gccagtgaca ccacggccta tgtgaactcc
    10861 ttccgcaacg tgatcatgga cctgagcaag tacgggggcc tcaagcgaac cccggtgatg
    10921 gacaacgtcg cccaggaagt cgacctgctg accggccagg tgatctggga atggcattcg
    10981 ctcggcaaca tccgggtacc ggagacctac aaggacatcc cgagcaagat cacccgcccc
    11041 ttcgactact tccacctcaa ctcgatttcc cgtgattccg acggcaacat gctgatctcg
    11101 gcccgtcaca cctgcgcgct gtacaagatc aaccgcgaga ccggcaaggt catgtggcga
    11161 atgggcggca aggactcgga cttcaaactg gccagcggtt tccgcttctg ttaccagcat
    11221 gacttccggc cccaccccaa cggcacctac tcgatcttcg agaacggcgc cgccgatggc
    11281 gaactgaagc cgaaggccag gatgtcgaag gcctacatct tccggatcaa ccagaagaag
    11341 aagaccaccg ccctggtcaa gacctacatc caccccggca acctgctctc accgagccag
    11401 ggcaacacgc aactcctgcc gaacggcaac gtcttcaccg gctggggctc gctcgagtac
    11461 tgcaccgagt tcgcccccga cggcgaggtg ctctgggaca tgcggtattc ggccgctgcc
    11521 gttacctacc gctgctaccg gtcaccctgg accggcaccc cggtgcccgg cgcgatcgga
    11581 gtcaagagtg aaaccgacgg cccgaacagc cgggtctggg tgagctggaa cggtgacacc
    11641 caggtcagga cctggcgggt cctggccggc aacccgggca acctcgccta ctcgacagaa
    11701 gtaccccgcg acgggttcga aacctcgatc ccggtgaccg gccagccggc cgcgttccga
    11761 ctcgtcggac tcgacggtca aggcaaggtg ctgggccggt ccagggtcaa caagctcgga
    11821 gagctcaccc ggtgaacctg gccctcgcga accgcaaccg ccgggcggcc ggatcagcgg
    11881 cggccgcact tgcgacagcc ctcgcggtcg tggtcgccgc gcttgccctt gccgcaccgg
    11941 ccggggcgaa gccgatcgcg gcctacccca gccccggatc cgtctatgcc tccccgacca
    12001 cgaacatcgc gctgagtggc gtcacccggg ccagcgtcgg ccggatcatg gtccgcggct
    12061 cgcggtccgg cttccaccgc ggccggatcg aagcctgggc gggtccggtc ggggtcagct
    12121 tcatccccag ccggcccttc gcgccgctgg agaaggtcac cgtgacctcg cgcagccacc
    12181 ccttctacgg gactggcggc tcccgctcat actcgttcaa gaccggcgag ttcctgccgg
    12241 aaaacctggg ggccgacccg ttcagcccgg ccaaggggca gaccccccgc gcctcgcaga
    12301 cctacaagac actgcgactc aaagtgccga agatcgtcgt ccacgcgaac gagccgggca
    12361 aatccaacgg caagatcttc tatgccccgc ggaccagcgg cccgaccatt ctcgacgccg
    12421 atggcaacct cgtctggtac cgccccggcc tgcggatcac cgacttccgg gcccaggtct
    12481 acaacggcca ccggatcctc acctggtggc gtcgcgacac cttcggcaag cgcgtcacca
    12541 gcaggttcga gatggccaac cgccactaca aggtcttccg ccgcttcggc ggaggcaacg
    12601 gcttcacagg cgacccgcat gagttcaacc tgacttcccg cggcaccgcc ttcgtcaccg
    12661 cctacaagac ggccgtcgtt gacctgagca ggttcggcgg gccgcgtcgc gccttcctgc
    12721 tcgattacat cggccaggaa atcgacatca agaccggtct cgtggtctgg gaatggcacc
    12781 cgctcggcaa cctcccgatg aaccggacct acctgccgat cccgcgacgc aacacccgtc
    12841 ccttcgactg gttccacatg aactcgatca acgacgacaa tgacggcaac gtcctgatct
    12901 ccgcccgcca cacccaggcc ctctacaaga tcaaccgcaa gacgggtcgg atcatgtggc
    12961 agatcggcgg caagggcggg gacttcaaac tcggcaaggg ggttcggttc
//
LOCUS       DAOQKN010000053        12970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_2251_length_12970_cov_6.176539, whole genome shotgun sequence.
ACCESSION   DAOQKN010000053 DAOQKN010000000
VERSION     DAOQKN010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 12970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12970
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2251_length_12970_cov_6.176539"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1675
                     /locus_tag="PLJ64_05220"
     CDS             <1..1675
                     /locus_tag="PLJ64_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNC92919.1"
                     /translation="INAVDLANQLEVGCFNHASSIAAAGLFKGTWREDFFDEAENLDK
                     HPYFRTKHESERIVRERLTVPWRVYRPGVVVGDSETGQMDKIDGPYYFFKLIQKLRDV
                     LPPWAPTIGVEGKQINVVPVNFVADAIDHISHQEGKDGMVFHLTDPNPLRAGQIINLF
                     ARSAHAPQMSMRLDPQMLEVIPPAVRGGLMMLPPVKRITDSVLDDLGIPREVLVYINY
                     PTKFDCTNTLEALEGTDIAVPPLASYADKLWDYWERNLDPDLFKDRSLGGAIGGKVVM
                     ITGASSGIGEAAAMKAAQAGAKVLLVARSRDKLEEIQTLIAEEGGEAFIHTADLSDTD
                     DIERMADEVLAEHGHVDVLVNNAGRSIRRSVALSYDRFHDYERTMQLNYFGSLKLILK
                     LLPRMRERKSGHIINVSSIGVQTNAPRFSAYVASKAALDAFSRCIGSEIIDDKCHITT
                     IHMPLVRTPMIAPTKMYDAFPAISPDEAAEMITDAMIRKPKKVATRLGNFGELMYSLS
                     PKASDAILNTGYKLFPDSKAAKGDKDGETEKAPSTEAVAFAHLLRGVHW"
     gene            1758..3404
                     /locus_tag="PLJ64_05225"
     CDS             1758..3404
                     /locus_tag="PLJ64_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNC92920.1"
                     /translation="MTDRRLKHWGWGYEDQQPSHEEIVAAAEGIIPMLGFGQAPERPA
                     ELDQVELRQSRLDSPGGIFTDEKHQRTVHALGKAYRDIVRGFYGQFDNPPDLVAFPED
                     EAGVEEVLSRAAGWGAAVIPFGGGTSVCGGVEPRLADPDRPVVSLDMTRMNRLVEVDE
                     TSLAARIQAGARGPELEDQLKAHGLTLRHFPQSFEYATLGGWIVTRAGGHFATGETHV
                     DDLTESIRAITPAGEWQSRRLPGSGAGPSPDRMLLGSEGILGVVTEAWMKVRPRPDFK
                     ATATVEFPPGDAAFSEAARGAVRLIAQSRLMPSNLRLIDSSEAATTMAGDGSATLLII
                     GFESHGQPVEDRMDQAVAIAEGEGGRVASRSSSTPSDRAGNKGGGGEDEAGADRWRSA
                     FLMAPYMRDTFVACGIIAETFETAITWDRFEEFHRSVIETTRRAVAEVTGAPSEGEGA
                     PRVSCRLTHVYPDGAAPYFTVLAPARRGSEVAQWDEIKAAASQAIIENGGTITHHHAV
                     GRDHRPWYDQQRPDQFAAALTAAKSAVDPDHMLNPGVLID"
     gene            complement(3478..4035)
                     /locus_tag="PLJ64_05230"
     CDS             complement(3478..4035)
                     /locus_tag="PLJ64_05230"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC92921.1"
                     /translation="MTTSTREAMLGSAIRLFRERGVGDTSFADVLAASGAPRGSVYHH
                     FPGGKKQLVQEAIESAGGYVAANFERSEDGPAEMVDRFARFYISELERTDFREGCPLM
                     AAAVAGAKEEAAVPAANAFSQIHEALTDSLTGAGVKKPRASSLATLAISAIEGAIILS
                     RTEKSTEPLNQTRQELHDIYAAALP"
     gene            complement(4103..4543)
                     /locus_tag="PLJ64_05235"
     CDS             complement(4103..4543)
                     /locus_tag="PLJ64_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HNC92922.1"
                     /translation="MELTYEEGALTPEARDQLVENLTTNLLKAEGAPNTEFFRSVTWV
                     LINERPAALTYAGDKPGAGVVKLDVITPEGALNDDRREQMIQAGTATIREAFGIADED
                     ALKVWVLCRDVVDGGWGAGGNVIHYKELVEIAKAQRAEREAAAA"
     gene            4852..5757
                     /locus_tag="PLJ64_05240"
     CDS             4852..5757
                     /locus_tag="PLJ64_05240"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: protein motif:HMM:NF008978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HNC92923.1"
                     /translation="MEASPQYTLTERGPLRAALKTARPQEWVKNVLVFAGLIFSGQLD
                     DTWAIGQALITFACFCAISSAGYFINDLNDVELDRQHPKKRFRPIAAGQLKVSTAWIL
                     SVVLAAGAIGVAFGVVNWKVGCMVAGYGVIQVAYSFGLKQVVIIDVMTLATLFILRVM
                     AGAEAVDAHASEWLILCTGMLACFLGFTKRRQEAVSELHEGTSSRPVLEHYSLPFLDQ
                     MVAMVTTGTVVSYAIYTVNSPLAGSEMMLTIPPVVYGIFRYLYLIYDRSDDRSTAAIV
                     AEDRGVIIAGAVFVAVALLVLYAFD"
     gene            6205..10794
                     /locus_tag="PLJ64_05245"
     CDS             6205..10794
                     /locus_tag="PLJ64_05245"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018262 - isopeptide cross-linking
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HNC92924.1"
                     /translation="MNFPARIIARVVIVAAILGLGFASTAQADRSFSVRYTTNDTGSI
                     KGIANTNMTCPSSASGCSTAQTQDPTSSANSYYNNNYDMTYVDVDSDSSTFNSSTANQ
                     SLPSGSTILFAGLYWGGDYSTGSASAPNSSDRNKVKLKLPGSSTYTTITASTLDDSTL
                     NVGRYQAFADVTSLIQALPNAGNGTYGVANIQAGKGSDHYAGWTLIVAYRNTAETAKN
                     MSIFDGLVTIRPSDPPTTIPFSGFLTPPSGPVNPQIGFVTWEGDHGLVGDSASLNGNT
                     LSDAQHPATNFFDSRISYNGVLYTDRNPNYANALGIDAAWTTPPPGTVGNSETSATLR
                     VTTSGDQYLPGVITFQTEIYAPKIDQTKSVTDDNGGNVRQGDTLTYKIYAKNNGQDGT
                     SNFVLRDPIPPNTTYVPGSINITKNTNASTGAKTDASSDDIAEYDSANSRIVARLGEG
                     ATTSVGGNVKPDSEYEVTFKVKVNGPTVNPVPQGTVLTNTATASFNSRATGTPLTATS
                     TATATVTATPNLKITKVRTGAAFTAGGNSAYTLTVNNHGDAPTSGTVSVTDPIPSGLT
                     VTAISAPGWTCNSVPASSLSCSRSDALAAGASYPNIVLTVAIDSGVSGEVENTSTVSG
                     GGDSDLSDNTSSSTNPVSNVADLGLTKTASKDEVLIGENFTYALTVRNYGPSKATSVA
                     ITDELPAGLSFVSATPAPGCAIQPVTGTLGCEVGTLNSGASYTVTVTVHVDGSAHGVL
                     HNTATVGAQQTDPNPGNNSGSDDVEVLGADLQVTKVKKSPATVVTGNTVVYEIKVKNL
                     GPSNATGVVLTDALPAGLSGVTTNQGACTVAAPNVSCVVGNLASGSTWTVEVSGTVAV
                     GTTELTNNASATGNEFDPNLSNNHDDVTTPVGKSADLSIVKNADPAQVVAGGNILYTF
                     VVSNAGPDEATGITITDTLPAGVTYVDGAPGCSAAGQVVTCTLNPIAAGGSRQTGITV
                     SVSPSATGVITNAAHVTGNENDPDPSNNDDSVDTPVKANADVEITKTASPANPVSGDT
                     VTYTLKAKNNGPDPAANVVVTDVLPAGVSFVSADSPCTEASGTVTCALGTLAAGEEVT
                     LEVKVTVDHWGDTDPSADHLLDVQKVENQIDLNPGEQKTIQVTCPSGYFATDGSVRID
                     EIDQGTGDWTAPQVLENRASSLDTWQGTVRNTAAGRAQAKIFAVCVRKTTGDENGHHH
                     NLLVSAPVTVTDSLVPGNNTKVLQCGPGQVAIQPGFIADKAGHLVYSQPEGNGWKFIY
                     KVEEGGTASFSIRCMDRQVDVVNGHTHDLKLQRISTEVEVAPGTVNEAQLTCPDGSKG
                     IVAGWDLDDGLVSLGNDPRPVTRAFKLYNPTDHTLSARLSLLCLGLRTGGERVPSKTI
                     INTANISTSSTESETGNNGSSAGINVNGIDNTPIDNDPDPDKPHPNNPIAKTIVGKGV
                     SYSASGVSFSLKCSGTCGGFAKLTTLRKLKVKGKKYGRGTVLARRQYFIGKAGTKKVK
                     LTLTGKGRKILKSGKAHRALLSISGGTRKVVKVGH"
     gene            11150..>12970
                     /locus_tag="PLJ64_05250"
     CDS             11150..>12970
                     /locus_tag="PLJ64_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92925.1"
                     /translation="MNSVSRVVFRIVAVAALLFAGLFAASSAQAAGTPDLQLSASSSN
                     PLYGENGNVSVTAALGSGQPKGYNLSFRVVLPAGISYAGGAAYAPTVLSNSPTTGKTT
                     LIFSNISDLVEDSSQSVSFQVAHDQAQYDVGNTYDIQYEAFLNTDPRIMPAFNADGTA
                     KPASYTGSGSATTTSKINAIKISKSEPSREGEILRGVHDHQTIYTLKVQNNYVNATNG
                     TKLNDWLPADLEFLGCEGDPDNTTNAPTNPGSAEEYPGSGPIVVQPVTDCHKPSLVET
                     VQADPDGAGPMPNAVYTHVQWDTGDLAPGQLITYRYRAAVPLTENTLNWSGATPATTG
                     AQGANLDNNAIAPPKEIQDEQKIVNYAIASGDYQSDVVTPFSVFDDTTLQRTAEDLVV
                     YKSNLTSSTLAQGDVNTWELRFRTGEYRYSDDIVVTDTLPSGYCPLGPVNYTTGNSPS
                     DSECDPTGDNPSVPNKSVTENADGTFTIVWDKSVMAKLGHTDVSDEFVITFPTKTRKN
                     YQQNFQDTTPILAADSTSNKVDLTANAFSRCTSPGTPDCATPGPRIWNDGQEPEPVVD
                     ASSAGQTAPQVTLKKEVAESGTNCQTATWVTNIPTYHPGDT"
BASE COUNT         2458 a         4521 c         3886 g         2105 t
ORIGIN      
        1 gatcaacgcg gtcgacctcg ccaaccagct tgaggtcggc tgcttcaacc acgcctcctc
       61 gatcgccgcc gccggtcttt tcaagggcac ctggcgcgag gacttcttcg acgaggccga
      121 gaacctcgac aagcacccct acttccggac gaagcatgag tcggagcgga tcgtccgcga
      181 gcggctgacc gtgccgtggc gggtctaccg tccgggcgtg gtggtcggtg attccgaaac
      241 cggccaaatg gacaagatcg atggtcccta ctacttcttc aagctgatcc agaagctgcg
      301 tgacgtgctg ccgccctggg cgccgacgat cggggtcgag ggcaagcaga tcaacgtcgt
      361 tccggtcaat ttcgtcgccg acgcgatcga ccacatcagc catcaggaag gcaaggacgg
      421 gatggtcttc cacctgaccg accccaaccc gctgcgggcc ggccagatca tcaacctttt
      481 cgcccgttcg gcccatgccc cgcagatgtc gatgcgactc gacccgcaga tgctcgaagt
      541 catcccgccc gcggttcgcg gcggcctgat gatgctgccg ccggtaaagc ggatcaccga
      601 ctcggtgctc gacgacctcg gcattccccg cgaggtgctc gtctacatca actatccgac
      661 caagttcgac tgcaccaaca ccctcgaggc cctcgaaggc accgacatcg cggtgccgcc
      721 gctcgcttcc tatgcggaca agctttggga ctactgggag cggaacctcg atccggacct
      781 gttcaaggac cgttcgctcg gcggagcgat cggcggcaag gtcgtgatga tcacgggtgc
      841 ctcatctggt atcggcgagg cagcggcgat gaaggcagcc caggccggcg ccaaggtgct
      901 gctggtcgcc cgcagccgcg acaagctgga ggagatccag accctgatcg ccgaggaggg
      961 cggcgaggcc tttatccaca cggccgacct ctctgacacc gatgacatcg agcggatggc
     1021 cgacgaggtg ctggccgagc acggtcacgt cgatgttctg gtcaacaacg ccggccgctc
     1081 gatccgccgt tcggtcgcgc tttcctacga tcgtttccac gattacgagc ggaccatgca
     1141 gctcaactac ttcggctcgc tgaagctgat cctcaagctg ctgccgcgga tgcgcgagcg
     1201 gaagtccggc cacatcatca acgtctcctc gatcggcgtc cagaccaatg cgccccgctt
     1261 ttcggcctat gtcgcctcga aggcggcgct ggacgccttc agccgctgca tcggctcgga
     1321 gatcatcgac gacaagtgcc acatcacgac gatccacatg ccgctggtgc ggaccccgat
     1381 gatcgcgccg accaagatgt atgacgcctt cccggcgatc agcccggacg aggcggcgga
     1441 gatgatcacc gacgcaatga tccgcaagcc gaagaaggtc gcgacccgtc ttggcaactt
     1501 cggcgaactg atgtacagcc tctcgccgaa ggcttcggat gcgatcctca ataccggcta
     1561 caagctcttc ccggattcga aggcggccaa gggcgacaag gacggcgaga ccgaaaaggc
     1621 gccatcgacc gaagcggtcg ccttcgccca cctcctgcgc ggcgtccact ggtaggcccg
     1681 ttgaccgaat cgagaaggca aaagtggcca tatagcgctc ccttttgcca tctcgattcc
     1741 cgtgaccggg agcagacatg acggatcgcc ggctgaagca ctggggctgg ggttacgagg
     1801 accagcagcc ttcccatgag gagatcgtcg ccgcggccga aggcatcatc ccgatgctcg
     1861 gcttcggcca ggccccggag cggccggccg aactcgacca ggtcgaactg aggcagtccc
     1921 ggctcgattc ccccggcggc atcttcaccg acgaaaagca ccagcgcacg gtccacgccc
     1981 tcggcaaggc ctaccgcgac atcgtccgcg gcttctacgg tcagttcgac aacccgcccg
     2041 atcttgtcgc cttcccggag gatgaagccg gggtagagga ggtgctctcc cgggcggccg
     2101 ggtggggcgc cgcggtgatt cccttcggtg gcggcacctc ggtctgcggc ggggtcgaac
     2161 cgcgactggc cgacccggac cggccggtgg tctcgctcga catgacccgg atgaaccggc
     2221 tggtcgaggt cgacgaaacg tcgctcgccg cccgcatcca ggccggggcc cgcggcccgg
     2281 aactggaaga ccagctcaag gcacacggac tgaccttgcg tcacttcccc cagtccttcg
     2341 agtacgcgac cctgggcggc tggatcgtga cccgggccgg cggccacttc gcgaccggcg
     2401 aaacccacgt cgatgacttg accgaatcga tccgcgcgat caccccggcc ggcgagtggc
     2461 agtcccgccg cctgcccggt tccggcgccg ggccttcccc ggaccggatg ctgctcggct
     2521 ccgagggcat cctcggagtg gtaaccgagg cgtggatgaa ggttcggccc cgaccggact
     2581 tcaaggcgac cgcgacggtc gagttccctc ccggagatgc cgccttctcc gaggcggccc
     2641 gtggcgcggt ccggttgatc gcccagtcgc ggttgatgcc ttccaacctg cgcctgatcg
     2701 attcctcgga ggccgcgacg acgatggccg gtgacggttc ggcgaccctg ctgatcatcg
     2761 gcttcgaatc ccacggccag ccggtcgaag accgcatgga ccaggccgtg gcgatcgccg
     2821 agggcgaggg cggccgggtt gcttcgcgca gttcatcgac cccgtccgac cgggccggca
     2881 acaaaggggg cgggggagaa gacgaggccg gcgccgaccg ctggcgttcg gccttcctca
     2941 tggccccgta tatgagagac accttcgtcg cctgcgggat catcgccgag accttcgaga
     3001 ccgcgatcac ctgggaccgc ttcgaggagt tccaccgcag cgtgatcgaa accacccggc
     3061 gcgccgtcgc cgaggtcacc ggcgccccga gcgaaggcga aggtgccccg cgggtcagct
     3121 gtcgcctcac ccacgtctac ccggacggcg ccgcccccta cttcacggtc ctcgcgcctg
     3181 cccgacgagg cagcgaggta gcccagtggg acgaaatcaa ggccgccgcc tcgcaggcga
     3241 tcatcgaaaa cggcggaacg atcacccacc accacgcggt gggcagggac caccgcccct
     3301 ggtacgacca gcagcgcccc gaccagttcg ccgctgccct caccgccgcc aagtcagccg
     3361 tcgaccccga ccacatgctc aacccgggcg tcctgatcga ttgagtgggg gctggggaat
     3421 cgagatggca aaagggagcg ctatatggcc acttttgcct tctcgatcta atgtggctca
     3481 ggggagcgcg gcggcgtaga tatcgtggag ttcctggcgg gtctggttga ggggttcggt
     3541 tgatttctcg gttcggctga ggatgatcgc tccctcgatt gccgagattg cgagggtggc
     3601 gagggagctg gcccggggtt tcttcacccc ggccccggtg agggaatcgg tgagggcttc
     3661 gtggatctgg ctgaaggcgt tggcggcggg gacggcggcc tcttccttgg ctccggcgac
     3721 cgcggcggcc atcagggggc agccttcgcg gaaatccgtg cgttcaagct cgcttatgta
     3781 gaagcgggcg aagcggtcga ccatctcggc cggcccgtcc tcgctccttt cgaaattggc
     3841 ggcgacatag ccgccggccg actcgatcgc ttcctggacg agctgcttct tgccgcccgg
     3901 aaagtggtgg tagaccgacc cccggggggc gccgctcgcg gcgagcacgt cggcgaagga
     3961 ggtgtcgccg accccccgct cgcggaagag gcggatcgcc gagccgagca tcgcttcgcg
     4021 ggtgctggtt gtcatggtcg aaagagatcc gttccggatg gcggggcgcc ggctggctgt
     4081 gcggccagcc ggcgcctcag ggtcaggccg ccgcggcttc ccgttcggcc cgctgggcct
     4141 tggcgatctc gaccagctcc ttgtaatgga tgacgttgcc gcccgcgccc catccgccgt
     4201 ccaccacgtc gcggcaaagc acccagacct tgagggcatc ctcgtcggcg atgccgaagg
     4261 cctcgcggat cgtggcggtg ccggcctgga tcatctgctc gcgccggtcg tcgttgagcg
     4321 cgccctcggg cgtgatcacg tccagcttca ccacacctgc tccgggcttg tcaccggcgt
     4381 aggtgagcgc ggcgggtcgc tcgttgatca ggacccaggt cacgctgcgg aagaactcgg
     4441 tgttcggggc accctcggcc ttgagcaggt tggtggtcag gttctcgacc agctggtcgc
     4501 gggcctcggg cgtcagggcc ccttcttcgt aagtcagttc catcaaaggc attggatttc
     4561 tccttggtcc gggaaggatc ggctcgggat tgagccgact atgcaggtat gcatacccta
     4621 tgcaggggtg catagtcaag tgccggggcg gagttcttgc cgggaagatt caccgagtgc
     4681 ccactttgtg ccggtatgtg gcgcaaaacg ggatctcgac gggtcggggt cggttggccg
     4741 cggcgctttc gatggactga ggcgtgcatc tggcgatgaa caggttgaac cggggcctga
     4801 gccggtagcc ttccccggcc gtgtcccgca gtcgggatgc gaaccccgac catggaagcc
     4861 tccccccaat acacactgac cgagcgcgga ccgttgcgag ccgccctgaa gacggcgcgg
     4921 ccccaggagt gggtcaagaa cgtcctggtc ttcgccggcc tgatcttctc cggtcagctc
     4981 gatgacacct gggcgatcgg ccaggccctg attaccttcg cctgcttctg cgcgatctcc
     5041 agcgccggct acttcatcaa cgacctgaac gacgtcgagc tcgaccgtca gcatccgaag
     5101 aagcggttcc ggccgattgc ggcggggcag ctcaaggtca gcactgcctg gattctctcg
     5161 gtggtgctcg ccgcgggtgc gatcggggtc gccttcgggg tggtcaactg gaaggtcggc
     5221 tgcatggtcg ccggttacgg cgtgatccag gtcgcctaca gcttcggcct caaacaggtc
     5281 gtgatcatcg atgtgatgac cctcgccacg ttgttcatcc tccgggtgat ggccggcgcc
     5341 gaggccgtgg atgcccacgc ctcggagtgg ctgatcctct gcaccggcat gctggcctgc
     5401 ttcctcggct tcaccaagcg gcgccaggaa gcggtatccg aacttcacga gggaacaagc
     5461 agccgaccgg tgctagagca ctactccctg cccttcctcg accagatggt cgccatggtg
     5521 actaccggca ccgtggtcag ttacgcgatc tacaccgtga actcgcccct ggccggttcc
     5581 gagatgatgc tgacgattcc ccccgtcgtc tacggcatct tccgctacct ctatctgatt
     5641 tacgaccgct cggatgaccg ttcaaccgcg gcgatcgtgg ccgaggaccg gggagtgatc
     5701 atcgccgggg cggtcttcgt tgcagtcgcc ctgctggtcc tctacgcctt cgactgatcc
     5761 aggccggcgg gcagaatccg gtcgtccgat tcgggccgga ggtgcctgac ttgcggtcga
     5821 ccggctcaag ccggcaaagc aggcaaatcc cggctatttg cgggcacatt caaggcagtc
     5881 cgccgagccc gataaccggt aaacccgtgc tgtaccgccg attgcgggta attcgcattt
     5941 ttcatatcag tcggtccgga cacttgacac gcatcagaaa acgcgtattc tcaatcgacc
     6001 aataagaaca cgggagacgg gtgcggaccc ccttcgagag ccggttggaa accggcaggg
     6061 atccagaaaa gacactctgt caactgctga atctcgatac ttgcgcagtt gaccgggcct
     6121 tcgaggcttt cccaccgttc agccgggcca ggaaatggct cgaacgaaac cggtgtaccc
     6181 aaaagagaaa cctcggagaa ctgaatgaat ttccccgctc gaatcatcgc ccgcgtcgtc
     6241 atcgtcgccg ccattctcgg cctcggcttc gcttccactg cccaggctga ccgcagcttc
     6301 agcgttcgct acacgaccaa tgacaccggg tcgatcaagg gcatcgcgaa caccaacatg
     6361 acctgcccct cgagcgcatc gggctgttcg accgcacaga cccaggaccc cacctcctcg
     6421 gcgaactcgt actacaacaa caactacgac atgacctatg tcgacgttga ttccgacagt
     6481 tcgaccttca actcctcgac ggctaaccag agcctgcctt ccggatcgac gattctcttc
     6541 gccggtctct actggggcgg cgattacagc accggctcgg cgtccgcgcc caactcgagc
     6601 gaccgcaaca aggtcaagct caagcttccc ggatcgagca cctacaccac gatcacggcc
     6661 agcacgctcg acgactcgac cctgaacgtc ggccgttacc aggccttcgc tgacgtaacc
     6721 agcctcatcc aggcgctgcc caacgcgggc aacggcacct atggcgtggc caacatccag
     6781 gccggcaagg gctcggatca ctacgccggc tggaccctga tcgtcgctta ccggaacact
     6841 gcggaaacgg ccaagaacat gtcgatcttc gacggtctgg tcaccatccg tccctcggac
     6901 ccgccgacca cgattccttt ctcgggcttc ctgacgccgc cttcgggtcc ggtcaacccc
     6961 cagatcggat tcgtcacctg ggagggtgac cacggcctgg tcggagattc ggcttccctg
     7021 aacggcaaca cgctctccga tgcccagcat ccggcgacca acttctttga ttcgcggatc
     7081 tcgtacaacg gcgtgctcta caccgatcgc aacccgaact acgcaaacgc gctcggcatc
     7141 gacgccgcct ggaccacccc gcccccgggc acggtcggca acagcgaaac ctcggcgacc
     7201 ctgcgggtaa ccacctcggg tgaccagtac ctgcccggcg taatcacctt ccagacggag
     7261 atctacgcgc cgaagatcga ccagaccaag tcggtcaccg acgacaacgg cggcaacgtg
     7321 cgccagggcg acaccctgac ctacaagatc tacgcgaaga acaacggcca ggacggcacc
     7381 tcgaacttcg tcctgcgcga tccgatcccg ccgaacacca cctacgtgcc cggctcgatc
     7441 aacatcacca agaacaccaa cgcctcgacc ggcgccaaga ccgacgcaag ctcggacgac
     7501 atcgccgaat acgactcggc caacagccgg atcgtcgccc gcctgggcga gggtgcgacg
     7561 acctcggtcg gtggcaacgt gaagccggac agcgaatacg aggtcacctt caaggtcaag
     7621 gtcaacggcc cgaccgtcaa tccggtcccg cagggcacgg tcctgaccaa caccgcgacg
     7681 gcttccttca acagccgggc caccggcacc ccgctgaccg ccacctcgac cgccaccgca
     7741 acggtgacgg caacgccgaa cctgaagatc accaaggtgc gcactggtgc ggccttcacg
     7801 gcaggcggca acagcgccta cacgctgacc gtcaacaacc acggcgacgc tcccacctcg
     7861 ggtacggtct ccgtgaccga tccgatcccc tccggactga ccgtaaccgc gatcagcgcg
     7921 cccggctgga cctgcaacag cgtcccggcc agttcgcttt cctgctcgcg cagcgatgcg
     7981 ctggctgccg gtgcgagcta cccgaacatc gtcctgaccg tcgcgatcga cagcggcgtc
     8041 agcggtgagg tcgagaacac ctcgaccgtc tccggcggcg gtgactccga cctgagcgac
     8101 aacacctcga gctcgaccaa cccggtctcg aacgtcgccg acctcggcct gaccaagacc
     8161 gcgtccaagg acgaggttct gatcggcgag aacttcacct acgccctcac ggtcaggaac
     8221 tacggaccct cgaaggcgac ctcggttgcg atcaccgacg agttgccggc cggcctcagc
     8281 ttcgtctcgg ccaccccagc tcccggctgt gcgatccagc cggtcacggg aacgctcggt
     8341 tgcgaagtcg gcaccctgaa ctccggggcg agctacaccg tcaccgtgac agtccacgtc
     8401 gacggttccg ctcacggtgt gcttcacaac accgcgacgg tcggcgccca gcagaccgac
     8461 ccgaacccgg gcaacaacag cggttcggat gacgtcgaag tgctcggcgc ggaccttcag
     8521 gtcaccaagg tcaagaagtc tccggccacc gtggtcaccg gtaacaccgt cgtctacgag
     8581 atcaaggtga agaacctcgg tccctcgaac gccaccggcg tcgtcctcac cgacgcgctg
     8641 ccggccggcc tgtccggcgt caccaccaac cagggcgcct gcaccgtcgc cgctccgaac
     8701 gtgagctgcg tggtcggcaa cctcgcttcc ggctccacct ggaccgttga agtcagcggc
     8761 accgtggcgg tgggcacgac cgaactgacc aacaacgcgt cagcgaccgg caacgaattc
     8821 gatcccaacc tgtccaacaa ccatgacgac gtcaccacgc cggttggcaa gtcggccgat
     8881 ctctcgatcg tcaagaacgc cgatccggcc caggtcgtcg ccggcgggaa catcctctac
     8941 acgttcgtgg tcagcaacgc cggtcccgat gaggcaaccg gaatcacgat caccgacacc
     9001 ctgcccgccg gagtcaccta cgttgatggc gccccgggct gcagcgcggc cggccaggtc
     9061 gtgacctgca cgctcaaccc gatcgccgcc ggtggttccc gccagaccgg gatcacggtc
     9121 agcgtctccc cgagcgcgac cggcgtgatc accaacgccg cccacgtcac cggcaacgaa
     9181 aacgatccgg acccgtcgaa caacgacgac tcggttgaca ccccggtcaa ggcgaacgcg
     9241 gacgtggaga tcaccaagac cgcttcgccg gccaaccccg tgtcgggtga cacggtcacc
     9301 tacacgctga aggcgaagaa caacggtccc gacccggccg ccaacgtcgt cgtgaccgac
     9361 gtgctcccgg ccggcgtcag cttcgtcagc gccgactcgc cctgcaccga ggcctccggc
     9421 acggtcacct gtgcccttgg cacgctggcc gcgggcgagg aagtgacgct cgaagtcaag
     9481 gtcacggtcg accactgggg cgacaccgac ccctcggccg atcatctgct ggacgtccag
     9541 aaggtggaga accagatcga cctgaatccc ggcgagcaga agaccattca ggtcacctgc
     9601 ccctccggtt acttcgcgac cgacggttcc gtccggatcg acgaaatcga tcagggcacc
     9661 ggtgactgga ccgctccgca ggtcctcgaa aaccgggcca gctcgctgga cacctggcag
     9721 ggcacggtcc gcaacaccgc ggccggtcgc gcccaggcca agatcttcgc ggtctgcgtc
     9781 aggaagacca ccggcgacga gaacggccac caccacaacc tgctggtcag cgccccggtc
     9841 accgtgaccg actcgctggt cccgggcaac aacaccaagg tgctgcagtg cggccccggc
     9901 caggttgcga tccagcccgg cttcatcgcc gacaaggccg gtcacctggt ctacagccag
     9961 cccgaaggca acggctggaa gttcatctac aaggtcgaag agggtggcac cgcctccttc
    10021 tcgatccgct gcatggaccg ccaggtggat gtcgtgaacg gtcacaccca tgacctgaag
    10081 ctccagcgca tctcgaccga ggtcgaggtt gcaccgggca cggtcaacga ggcgcagctc
    10141 acctgcccgg acggcagcaa gggaatcgtc gccggctggg acctcgacga cggtctcgtt
    10201 tcgctcggca acgatccgcg tccggtcacc cgggccttca agctctacaa cccgaccgac
    10261 cacacgctga gtgcccgcct cagtctgctc tgcctgggcc tccgtaccgg tggtgagcgg
    10321 gtgccgtcca agacgatcat caacacggcc aacatctcga ccagctcgac cgagtcggaa
    10381 accggcaaca acggctcttc ggccgggatc aacgtgaacg ggatcgacaa caccccgatt
    10441 gacaacgatc cggatccgga caagccgcac ccgaacaacc cgatcgccaa gacgatcgtc
    10501 ggcaagggcg tcagctactc cgcttcaggt gtcagcttca gcctcaagtg ctcgggtacc
    10561 tgtggcgggt tcgccaagct gaccacgctc aggaagctga aggtcaaggg caagaagtac
    10621 ggccgcggca cagttctcgc caggcgccag tacttcatcg gcaaggccgg cacgaagaag
    10681 gtcaaactga ccctgaccgg caagggccgc aagatcctga agagcgggaa ggcccacagg
    10741 gctctgctca gcatttctgg agggaccaga aaggtagtca aagtcgggca ctgagcctga
    10801 gacttactgc cagggagtaa aagccccggt cacaagaccg gggctttttg ctgcaactag
    10861 cgggattttg gttgcccgcg cacctacaat ttttcttctg gcgccaccgt aacttgacaa
    10921 gctacggatt gttcctatta ttgacagcca atctgaacac gggagacggg cttgattccc
    10981 tttgagaacc ggcaggcggc cgggggattc tgaaaaagct ctgtcaactg caattctcaa
    11041 ttcttgcggc agttgacctg gcctccgagg ctttcccacc gtttcacaca ggccatcaaa
    11101 gcaatggatc ggaccccaac cagggtgtcc acgaagtatc ggagaaaaat tgaattctgt
    11161 cagccgtgtc gtatttcgaa ttgtcgccgt agccgccctt cttttcgcgg gtcttttcgc
    11221 cgcatcctcg gcccaggccg cgggaacgcc cgaccttcag ctgtcggcat cctcgtcgaa
    11281 tccgctttac ggcgaaaacg gaaacgtctc ggtcaccgcc gccctcggtt ccggtcagcc
    11341 caagggctac aacctgtcct tccgtgtcgt gctgccggcc gggatctcct acgccggcgg
    11401 tgccgcctac gcacccacgg tcctgagcaa ctcgccgaca accggcaaga ccaccctgat
    11461 cttcagcaac atttccgacc tggtcgagga ttcgtcccag tcggtcagct tccaggtcgc
    11521 ccacgatcag gcgcagtacg acgtcggcaa cacctacgac atccagtacg aggctttcct
    11581 caacaccgat ccgcggatca tgccggcctt caacgcggac ggcaccgcca agccagcgag
    11641 ctacaccggt agcggttctg ccacgaccac ttcgaagatc aacgcgatca agatcagcaa
    11701 gtccgagccg agccgcgagg gcgagatcct gcgcggagtt cacgaccacc agacgatcta
    11761 cacgctgaag gtccagaaca actatgtaaa cgcgacgaat ggcaccaagc tgaacgactg
    11821 gctgcccgcc gacctcgagt tcctcggttg cgagggtgac ccggacaaca cgaccaacgc
    11881 gccgaccaat cccggctcgg ccgaggagta ccccggttcc ggcccgatcg tcgtacagcc
    11941 cgtcactgat tgccataagc cttccctggt cgagaccgtc caggccgacc cggacggcgc
    12001 cggcccgatg ccgaacgcgg tctacacgca tgtccagtgg gacaccggcg acctcgcccc
    12061 cggccagctg atcacctacc gctaccgtgc cgcggtcccg ctgaccgaga acacccttaa
    12121 ctggagcggc gccaccccgg cgaccaccgg ggcccagggt gcgaacctgg acaacaacgc
    12181 gatcgccccg ccgaaggaga tccaggacga acagaagatc gtcaactacg cgatcgcctc
    12241 cggtgactac cagagcgatg tggttactcc cttcagcgtc ttcgatgaca ccacccttca
    12301 gcgcaccgcc gaggatctcg tcgtctacaa gtcgaatctg acctcgagca cgctcgccca
    12361 gggtgatgtc aacacctggg agctccgctt ccgcaccggc gagtaccgct acagcgatga
    12421 catcgtcgtc accgacacac tgccttccgg ttactgcccg ctcggcccgg tcaactacac
    12481 gaccggcaac agcccttccg attccgaatg cgacccgacc ggcgacaacc cgagcgtgcc
    12541 gaacaagagc gtgaccgaga acgccgacgg caccttcacg atcgtctggg acaagtcggt
    12601 catggcgaag ctcggccaca ccgacgtgag cgacgaattc gtcatcacct tcccgacgaa
    12661 gactcgcaag aactaccagc agaatttcca ggacacgacg ccgattctgg ccgccgactc
    12721 gacctccaac aaggtcgacc tgaccgccaa cgcgttctcg cgctgcacct cgcccggcac
    12781 cccggactgc gccacccccg gcccccggat ctggaatgac gggcaggagc ccgagccggt
    12841 ggtcgacgct tcctcggctg gccagaccgc ccctcaggtc accctgaaga aggaagtcgc
    12901 cgagtccggc accaactgcc agacggccac ctgggtcacg aacatcccga cctaccaccc
    12961 gggcgacacg
//
LOCUS       DAOQKN010000054        12967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_2252_length_12967_cov_5.281444, whole genome shotgun sequence.
ACCESSION   DAOQKN010000054 DAOQKN010000000
VERSION     DAOQKN010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 12967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12967
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2252_length_12967_cov_5.281444"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="PLJ64_05255"
     CDS             <1..586
                     /locus_tag="PLJ64_05255"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNC92926.1"
                     /translation="KNRTIALAPWLILRHSSGLRPPYILPSGGSSPLGALSYVETAFE
                     IAAQVESGELPEPGHAVVPTGSGGTAAGLALGFALAGLGTRVVGVIVTEHLRLDDEAI
                     GKLAGKSAALLRQRGAAFPEPDLNFEMTEEWLGETYGAPTPESEAAIERARADGLELD
                     PVYTAKAMAALIAKSAAGDFEGGPVLFINTNGPR"
     gene            complement(583..1272)
                     /gene="nucS"
                     /locus_tag="PLJ64_05260"
     CDS             complement(583..1272)
                     /gene="nucS"
                     /locus_tag="PLJ64_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HNC92927.1"
                     /translation="MRLIVARCEARYTGRLDTFLPEALRLVMIKSDGSVMIHADTGGY
                     KPQNWMTPPTTIEEIEAEGDEPAMIVVTKHAGATEDRVEIKIAEVLSDVTHDMGEAAA
                     LERDGVEAHLQELLAEQPHWCGEGLTLVKREWFTDIGPVDLMCKDEDENWVAVEIKRT
                     GTVEAVEQITRYLERIQLEPAMAACRGVLAAQQIKPQARTLAEGRDIACVEVDLAVLR
                     GEREPDLTLFG"
     gene            complement(1384..2061)
                     /locus_tag="PLJ64_05265"
     CDS             complement(1384..2061)
                     /locus_tag="PLJ64_05265"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:NF003588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNC92928.1"
                     /translation="MPARPLSQVIDPGWAEALEPVSGQIASMGDFLRGESAAGRGYLP
                     AGENILRAFTLPLDQVRVLIVGQDPYPTPGHAVGLCFSVAPDVRPLPPSLVNIFKEYS
                     DDLGYPPPENGDLSPWFDRGVLLLNRSLSVQPGRPNAHQGKGWEGVTEQAITALARRG
                     GPLVAILWGRNARNLAPLLGPVPRIESAHPSPMAAHNGFFGSKPFSRANQLLADQGAE
                     PIDWRLP"
     gene            complement(2104..4323)
                     /locus_tag="PLJ64_05270"
     CDS             complement(2104..4323)
                     /locus_tag="PLJ64_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNC92929.1"
                     /translation="MSETKTIQWEKGDDGIVILTIDDPSQGANTMNRDYIESMGQIVD
                     RLDAEKESVTGVIITSGKKTFFAGGDLNDLLAVTKEQSVEFAAGIREVKSQLRRLETL
                     GVPVVAAVAGSALGGGLEITLATHHRIVVDDPKIQLGTPEVQLGLLPGGGGVVRTVRM
                     LGLADALMQVLIQGKPLRPAKAREIGLIDELVQSPDDLIPAAKKWIGENPEAVQRWDV
                     KGYKIPGGTPSSPKLAMTLPAFPSNLKKQLKGAKYPAPIAIMSAAVEGSQVDFDTAIE
                     IEGRYFLELATGQIAKNMIQAFFFDLQEVNGDRGRPADGERYTAKKVVVLGAGMMGAA
                     IAYVCAKAGIEVVLKDISLEAAERGKGYSKDIVDKAISRGRSTQEKGDALLALITPTA
                     EAKDADGADLVIEAVFEDPAVKAQVFAEIEPFLAEGALLGSNTSTLPITGLAEGVSRP
                     ADFIGLHFFSPVDKMPLLEIIKGEETSDTTLHRSLDLARQIRKTPIVVNDSRGFFTSR
                     VIGTFINEGIAFLAEGVPASTIEQASSQAGYPAPVLQLSDELNMELMIKIRKATREAL
                     EAEGVEWNPHPAFAVVDRMVEAGRAGKLAGAGFYDYEDGKRTGLWAGLREEFPPAGDP
                     SQIDLLELQERMLFVESLETVKCLDEGVIESVADANIGSIFGIGFPGWTGGVLQYIDG
                     YTSEAHPERRGPAGFVERSRELAEKYGERFEPPASLVEKAERGETYSGQPVRDLVAS"
     gene            complement(4352..5566)
                     /locus_tag="PLJ64_05275"
     CDS             complement(4352..5566)
                     /locus_tag="PLJ64_05275"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HNC92930.1"
                     /translation="MSTDALIFDAIRTPRGKGKSSGSLYSTKPVDLVVGLMHETLIRN
                     EKLDPNTIDDVVLGCVSPVGDQGADIAKTAAIKAGLPDTVAGVQLNRFCASGLEAVNI
                     AAQKVASGWEDLVLAGGVESMSRVPMGSDGGAWAMDPETNYDTSFVPQGIGADLIATI
                     EGFTRDDVDEFAVRSQERAATAQKEGRFANSVIPVKDFNDMVVLDHDEFIREGTTTES
                     LAKLNPSFAGMGEMGGFDAVALQKYHWVEQINHVHHAGNSSGIVDGASLVAIGSEKAG
                     ERNGMTPRARIVATAVSGADPTIMLTAPGPASKKALEKAGLTADDLDLIEINEAFAAV
                     ALRLVRDLDVDMEKVNVNGGAIAMGHPLGATGAMILGTIVDELERTGGRYGLCTLCVG
                     GGMGIATIVERV"
     gene            complement(5638..6258)
                     /locus_tag="PLJ64_05280"
     CDS             complement(5638..6258)
                     /locus_tag="PLJ64_05280"
                     /inference="COORDINATES: protein motif:HMM:NF025343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC92931.1"
                     /translation="MPATGQSGTGRQREQSEVSRQKLLDAAAIVFARQGFHKASLQAI
                     GEEAGISRGSIVWHFGTKDALLEAVVERSYLSWAREVESSEAGKPGGRQAIVRVIEAH
                     RRFIEESPAENRLYYALIAEVSANEELAERVRKMHAELYLQLHGYIKEGQEAGEISSQ
                     LDPEVGARLVLSTLGGIGHLVILVPDADVGPIYREVLRSMEANLAI"
     gene            complement(6316..8073)
                     /locus_tag="PLJ64_05285"
     CDS             complement(6316..8073)
                     /locus_tag="PLJ64_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92932.1"
                     /translation="MRKRVYGRFLLALMLGLAGSFALASTAQAAFGLSGLVAAPANTD
                     AGAHADFHTHLEITSPEDQLRDLTVSLPPGLSGNPQSVAKCTADQLNADSCPAGSQIG
                     TTTVNLTAMSLVPLTVNGSVYNVAPEVGEPARLGIVLRPPDPTHLLLPPIVMQVQVKL
                     RSDFGLDTILTDLPQNAAVLNLLPVGIAINSVDLDLDGAFMRNPTSCGTKTAGFSARS
                     WAMADGDPAVTGSTTFESTNCAAQSYDPQASLHIDGNGKPLAQGSRPTVTSVITQTES
                     EANNQRADVVLPAGLGVDGGRFDQTCPLADFQAGNCPESTKVGEATAVSPLLDSDLSG
                     PVYAVGADGLPQLGVDLKGTLPLKILGSSGFRNGRVETIFEGLPDLPLSRFELVFPGG
                     PTGLIRVIGDVCAESSTFNADFTSQAGQTVSRTLPTTETGTCPSGNGGGNGNGNGNGN
                     GGNQVKKPKAKVKVSGAKNHRPRASIRVRSGGARMRVVQVKLPAALKLNKAAAKHLVA
                     KADGKRVRGGKLVAKGRTLKLRGLPGKGAGVVTVNIPPAAIAAPGRARKGSKLTFRLK
                     VKRTGQKPVSLKASARVTK"
     gene            8270..8992
                     /locus_tag="PLJ64_05290"
     CDS             8270..8992
                     /locus_tag="PLJ64_05290"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNC92933.1"
                     /translation="MAAVGDIHCREANREPLARSIAAISDADLLLIAGDITSHGTVEE
                     AQILCEVTAGVDFPVFAVLGNHDLHAGQAKEIIETLRAGGIDLLDGTSAISEIKGVEV
                     GVVGSRGFVGGFAPHHLPDFGEPSLRAVYEETSREVSALEAGLSEVATCQIRLVVLHY
                     SPLRATVEGEPPEIFPFLGSDRMARPLLEHGPDLVVHGHAHEGTHEGSIGEVPVRNVS
                     LPVLGGEIWITELNAPVHGPIP"
     gene            complement(8974..9600)
                     /locus_tag="PLJ64_05295"
     CDS             complement(8974..9600)
                     /locus_tag="PLJ64_05295"
                     /inference="COORDINATES: protein motif:HMM:NF021487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HNC92934.1"
                     /translation="MKESDPDKTEILTTSLKSAAAIMERESIPYLLGGGLGTWAHGGP
                     PSSNDIDLMVRPADAERAIQAFADAGLRTERPPEQWLVKAWDGDVLIDLIFQPAGMRI
                     TEQVIERAEELNVGGMVVPVMCIDDILTTKLLAMDEHAADFLTLIQIARALREQIDWP
                     SLVERTSSSPFARAFFVLVDGLGIQPLALPGPGGVEWQIGPGRQGIGP"
     gene            9829..10731
                     /locus_tag="PLJ64_05300"
     CDS             9829..10731
                     /locus_tag="PLJ64_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92935.1"
                     /translation="MKSGITVGLAALVACAGLVSSAHAAFPGKNGRIIFERKASQFAG
                     NSDPWVFSGGKARRLVRISNNAYNFSYSPNGKKIVFEATVPSEELVVMNANGTRPRVI
                     TARVHKCIGKRHPTWSPNGKKIAFVCLNRNSLSDHDVWSVNANGTGIRRISRTHDAYS
                     PAWSPRGNKIAYTTYGGSIYTVPARGGKSKLLIAEAPGGVFGGTFQGVDWSPNGRLLV
                     SDSSGDGVYTINARTGATSGDLAEIGAEPVFSPDGKKILYVGIAESRGSTRIGLWKMN
                     LDGSNKQQVTRGGYDRAPSWAPAR"
     gene            complement(10784..10856)
                     /locus_tag="PLJ64_05305"
     tRNA            complement(10784..10856)
                     /locus_tag="PLJ64_05305"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10821..10823),aa:His,seq:gtg)
     gene            10936..11592
                     /locus_tag="PLJ64_05310"
     CDS             10936..11592
                     /locus_tag="PLJ64_05310"
                     /inference="COORDINATES: protein motif:HMM:NF025510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HNC92936.1"
                     /translation="MSVRREMRGFLVGSLLFALGATPGYLGLVGVTADNLTYFLGSIF
                     FTIAGFTQLRLTGRWQRGGWSDRDAWADWWAAAIQFLGTLAFNVSTLAALLGTIDPGT
                     FERLGWRPDFIGSICFLVASVLAVEATTNRESLWDPEARNWWNTWLNMAGSVAFMISA
                     IFAWINPSTGEPLNTDWANLGTFFGAIGFFAAAALLKPQADADSNPSMNGTGPAENGD
                     "
     gene            complement(11589..12476)
                     /locus_tag="PLJ64_05315"
     CDS             complement(11589..12476)
                     /locus_tag="PLJ64_05315"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNC92937.1"
                     /translation="MAASNESRSPHPSPSLPAVAMVLGSISSIQVGAALATGLFDEVG
                     AMGVTLLRTLISAVLLVLIWRPSFRLDRGRTRIALLFGFVLAAMNLSFYEAIDRIPLG
                     IAVTFEFIGPLGVALITSHRRRDLIWVAMAAAGILLLSGGIGEEGIELFGVVLAVVAG
                     VFWGCYILLGKRVGEEFYGGQGLAIAMVFSTVICTPFGVADGGADLLLPSVLAVGFAV
                     AILSTTLPLSLEMEALRRLPSNVFGVMMSLEPAMAATIGFLLLGQDMSAAQIAAIALV
                     VSASAGALSSARAPAPIEP"
     gene            12536..>12967
                     /locus_tag="PLJ64_05320"
     CDS             12536..>12967
                     /locus_tag="PLJ64_05320"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNC92938.1"
                     /translation="MTDTPPTRDGYPHFLTIPTRWKDNDIYGHVNNVEYYSYFDTVIN
                     EYLIREGGLDIHGGGTIGVCAESHCRFLAAVAFPDPVEAGLRVGHLGSSSVRYEIGLF
                     AGNGEPLAEGWFVHVFVQRESRRPDGIPDSIRCALEKLEVHS"
BASE COUNT         2128 a         4369 c         4266 g         2204 t
ORIGIN      
        1 gaagaaccgc acgatcgccc tcgccccctg gctgatcctc cgccactctt ccggcctgcg
       61 gccgccgtac atcctgccct cgggaggttc ttcgccgctc ggtgcgctca gttacgtcga
      121 gacggcgttc gagatcgcgg cgcaggtcga gtcgggtgag ttgcccgaac cgggccacgc
      181 agtggtgccg accgggtccg gcggaaccgc ggcgggcctg gcgctcggct tcgccctcgc
      241 cgggctcggc acccgggttg tcggcgtaat cgttaccgag caccttcggc tcgatgacga
      301 agcgatcggc aagctggccg gcaagagcgc tgccctgctc cgccagcgcg gggccgcgtt
      361 ccccgagccg gacctgaact tcgagatgac cgaggagtgg ctcggggaaa cctacggcgc
      421 ccccaccccg gaatcggagg cggcgatcga aagggcccgg gccgacgggc tcgaactcga
      481 cccggtctac accgccaagg cgatggccgc cctgatcgcc aagtccgcgg ccggtgactt
      541 cgaaggcggg cccgtcctct tcatcaatac aaacgggccc cgttaaccga agagggtgag
      601 gtcgggttcg cgttcgccgc ggaggactgc gaggtcgact tcgacgcagg cgatgtcccg
      661 gccctcggcc agggtccgtg cctggggctt gatctgctgg gcggcgagca ccccgcggca
      721 tgccgccatg gccggctcca gctgaatccg ctccaggtag cgggtgatct gctcgaccgc
      781 ctcgaccgtc ccggtccgct tgatctcgac ggcgacccag ttttcatcct cgtccttgca
      841 catcaggtca accggtccga tgtcggtgaa ccactcccgc ttgaccaggg tcaggccctc
      901 gccgcaccaa tgcggctgct cggccagcag ctcctgaagg tgggcctcca ccccgtcccg
      961 ctccagggcc gccgcctcgc ccatgtcatg ggtcacgtcg gaaagcacct cggcgatctt
     1021 gatctcgacc cggtcctcgg tcgcaccggc gtgtttggtc acgacgatca tcgccggctc
     1081 gtcgccttcc gcctcgatct cctcgatcgt ggtcggggga gtcatccagt tctggggctt
     1141 gtagccaccg gtgtcggcgt ggatcatcac cgacccgtcc gacttgatca tcaccagccg
     1201 gagcgcctcc ggcagaaagg tgtccagacg gccggtatag cgggcctcgc aacgagcaac
     1261 gatcaaacgc acgatcggaa cagtagccaa gcctcaggtc gccgctggcc gcccgcgcgc
     1321 cgcgagtgcc cactttgcgc cgcataccgg cacaaagtgg gcattcggtg acccaccgcg
     1381 cctctacggg aggcgccagt cgattggctc ggccccctgg tcggccagga gctggttggc
     1441 ccggctgaag ggcttcgagc cgaagaagcc gttgtgggcg gccatcgggc tgggatgggc
     1501 cgattcgatc cgcggcaccg ggccgagcaa aggcgccagg ttgcgggcgt tccggcccca
     1561 gaggatcgcg accaacggcc cgccccgccg ggccagcgcg gtgatcgcct gttcggtcac
     1621 cccctcccag cccttgccct gatgcgcgtt cggccggccg ggctggaccg atagcgaccg
     1681 gttcagcagc agcaccccgc ggtcgaacca gggagaaagg tcgccgtttt ccggcggcgg
     1741 atagccaagg tcgtcgctgt actccttgaa gatgttgacc agactcggtg gcagcggccg
     1801 cacatccggg gcgaccgaga agcagagccc gacggcatgc cccggggtcg ggtacgggtc
     1861 ctgcccgaca atcagcaccc ggacctgatc gagcggcagg gtgaaggccc tgaggatgtt
     1921 ctccccggcc ggcaggtaac cgcggcccgc ggccgattcg ccccggagga agtcccccat
     1981 cgaggcgatc tgcccggaaa ccggttcgag cgcctcggcc cagccggggt cgattacctg
     2041 acttagcgga cgggccggca cgaacccaga atacgagttc gcgccggcgc cgcccgcgga
     2101 aggtcaggaa gcgaccagat cccggaccgg ctggccggag taggtctcgc cacgctcggc
     2161 cttttcgacc agggaagccg gcggctcgaa acgctcgccg tacttttcgg ccagctcccg
     2221 gctgcgctcg acgaagcccg ccgggccgcg gcgctcgggg tgggcttccg aggtgtagcc
     2281 gtcgatgtac tggagcacgc cgccggtcca gcccgggaag ccgatcccga agatcgagcc
     2341 gatgttggcg tcggccaccg attcgatcac gccctcgtcg aggcacttga ccgtttccag
     2401 cgattcaacg aaaagcatcc gctcctgcag ttcgagcagg tcgatctgcg aggggtcccc
     2461 ggccggaggg aactcttccc tgaggccggc ccagagcccg gtccgcttgc cgtcctcgta
     2521 gtcgtagaaa ccggcacccg ccagcttccc ggcccgaccc gcttcgacca tgcggtcgac
     2581 cacggcgaag gccgggtgcg ggttccactc gacgccctcg gcttccaggg cctcgcgggt
     2641 cgccttgcgg atcttgatca tcagctccat gttgagctcg tcgctcagct ggagcaccgg
     2701 ggccgggtag ccggcctggc tcgaagcctg ctcgatggtc gaggccggca cgccctcggc
     2761 gaggaaggcg atgccctcgt tgatgaaggt gccgatcacc cggctggtga agaagccgcg
     2821 tgaatcgttg accacgatcg gtgtcttcct gatctgccgg gcgaggtcga gcgagcggtg
     2881 gagcgtcgta tcgctggtct cttcgccctt gatgatctcc agcaggggca tcttgtcgac
     2941 cgggctgaag aagtgaaggc cgatgaagtc ggccgggcgt gaaacgccct cggccagacc
     3001 ggtgatcggc agggtcgagg tgttcgagcc aagcagtgcg ccttcggcca ggaagggctc
     3061 gatctcggcg aagacctggg ccttgaccgc cgggtcctcg aagaccgcct cgatcaccag
     3121 gtcggcgccg tcggcgtcct tcgcttcggc ggtcggggtg atcagggcca gcagggcatc
     3181 gcccttttcc tgggtcgaac ggccgcggga gatcgccttg tcgacgatgt ccttcgagta
     3241 acccttgccc cgctcggcgg cctccagcga gatgtccttt agcaccacct cgatgcccgc
     3301 cttggcgcag acataggcga ttgcggcgcc catcattccg gcacccagca ccaccacctt
     3361 cttcgcggtg taccgctcgc cgtcggcggg acggccgcgg tcaccgttca cttcctgaag
     3421 gtcgaagaag aatgcctgga tcatgttctt ggcgatctgg ccggtggcca gctcgaggaa
     3481 gtaacggccc tcgatctcga tcgcggtgtc gaagtcgacc tgcgaaccct cgaccgcggc
     3541 cgacatgatc gcgatcggcg ccgggtactt cgcacccttg agctgcttct tcaggttgga
     3601 agggaaagcc ggcagggtca tcgccagctt cgggctggac ggggtgccgc cggggatctt
     3661 gtagcccttc acatcccagc gctggaccgc ttccgggttc tcgccgatcc acttcttcgc
     3721 ggccgggatc aggtcatccg gcgactggac cagctcgtcg atcagcccga tctccctggc
     3781 cttggccgga cggagcggct tgccctggat cagcacctgc atcagggcat cggccagacc
     3841 gagcatccgt accgtgcgga ccactccgcc gccgccgggc agcaggccga gctggacctc
     3901 cggggtaccg agctggatct tcgggtcgtc gaccacgatc cggtgatggg tggcgagcgt
     3961 tatctcgagc ccgccgccga gcgccgaacc ggccaccgcg gccacgaccg ggacgccgag
     4021 cgtctcgagg cggcgcagct gcgatttgac ctcgcggatg cctgccgcga actcgaccga
     4081 ctgctctttg gtcacggcga gcaggtcgtt caggtcaccg ccggcgaaga aggtcttttt
     4141 gcccgaggtg atgatcacgc cggtgacgct ttccttctcg gcatcgaggc ggtcgacgat
     4201 ctgacccatc gactcgatgt agtcgcgatt catcgtgttc gcgccctggc tgggatcgtc
     4261 gatggtcagg atgacgattc cgtcgtcgcc cttttcccac tggatggtct tggtttcact
     4321 catgttcagg ttctcctggt ctttccctgg atcagacgcg ctcgacgatg gtcgcgattc
     4381 ccatgccgcc gccgacgcag agggtgcaga gcccgtaacg gccgccggtg cgctcgagct
     4441 cgtcgacgat cgtgccgagg atcatcgccc cggtcgcgcc gagcgggtgg cccatggcga
     4501 tcgcgccgcc gttcacgttg accttctcca tgtcaacgtc gaggtcgcgg accagtcgca
     4561 gcgccaccgc ggcgaaggcc tcgttgatct cgatcaggtc gaggtcgtcg gcggtgaggc
     4621 ccgccttctc gagcgccttc ttgctggccg ggccgggcgc ggtgagcatg atcgtcgggt
     4681 cggctccgga aaccgcggtg gcgacgatgc gggcccgcgg ggtcatgccg tttcgctcgc
     4741 cggccttctc gctgccgatc gcgacgagcg aggcgccgtc gacgattccg gaagagttgc
     4801 cggcgtggtg gacgtggttg atctgctcga cccagtggta tttctgaagc gcgacggcgt
     4861 cgaatccgcc catctcgccc atcccggcga aagagggatt cagcttggcc agcgactcgg
     4921 tcgtggtgcc ctcgcggatg aactcgtcat ggtcgagcac gaccatgtcg ttgaagtcct
     4981 tgaccggaat cacggagttg gcgaaacggc cttccttctg ggctgtcgcg gcccgttcct
     5041 gtgagcggac ggcgaactcg tcgacgtcat cccgggtgaa accttcgatc gtcgcgatca
     5101 ggtcggcccc gatcccctgc gggacgaaac tggtgtcgta attggtctcg gggtccatcg
     5161 cccaggcacc gccatcggag cccatcggta cgcgcgacat cgattcgacg ccaccggcga
     5221 ggaccaggtc ctcccagccc gaggcgacct tctgggcggc gatgttcacc gcttcgaggc
     5281 cggaggcgca gaagcggttg agctggactc cggcaaccgt gtccggcagg ccggccttga
     5341 tcgccgcggt cttggcgatg tcggcgccct ggtcaccgac cggtgaaacg cagccgagca
     5401 cgacgtcgtc gatggtgttg ggatcgagct tttcgttgcg gatcagggtc tcgtgcatca
     5461 gtccgaccac gaggtcgacc ggcttggtcg agtaaagcga gccgctgctc ttgcctttgc
     5521 cgcgcggtgt gcggatggcg tcgaagatca gtgcgtcggt gctcatcggg aggctccgtt
     5581 tcggaagaga agtgattttt ctgtgctggc tgacacagga atggccccaa aattatgtca
     5641 aatagccaga ttcgcctcca tgctgcggag cacttcccga tagatcggac caacatccgc
     5701 atccggcacg aggatcacca ggtggccgat cccgcccagg gttgaaagca ccagcctcgc
     5761 cccgacttcc gggtcgagct ggctcgagat ctctcccgcc tcctggcctt ccttgatgta
     5821 gccgtggagc tgcaggtaga gctcggcgtg catcttccgc acccgctcgg ccagctcctc
     5881 gttggcgctc acctcggcga tcagggcgta gtagagacgg ttctcggccg ggctctcctc
     5941 gatgaaacgg cgatgcgcct cgatcaccct gacgatcgcc tggcgaccgc ccggctttcc
     6001 ggcttcgctg gactcaacct cccgggccca ggaaagatag gagcgctcga ccaccgcctc
     6061 gagcagggcg tccttggtcc cgaaatgcca gacgatcgag ccccggctga tcccggcctc
     6121 ttctccgatc gcctgaagcg aggccttgtg aaagccctgc cgcgcgaaca cgatcgcggc
     6181 cgcgtcgaga agcttctgcc gggatacctc ggactgctcc cgctggcgcc cggttccgga
     6241 ttgacctgtg gctggcacgc ggcaagtatc gcgactcctc cggctgcggg cggccctgcc
     6301 gccacccgcg gccggctatt tagtgacccg ggcggaagcc ttgaggctga ccggtttctg
     6361 ccccgtccgc ttgaccttca gccggaaggt cagcttgctg cccttgcgcg cacgcccggg
     6421 agcggcgatc gccgccggcg gaatgttcac cgtcacgaca ccggctccct tgccgggcag
     6481 cccgcgcagt ttgagggtcc tgccctttgc cacgagcttc ccgccccgga cacgcttgcc
     6541 gtcggccttg gctacgaggt gctttgccgc ggccttgttc agcttgagcg ccgccggcag
     6601 cttgacctgg acgacgcgca tgcgggcccc gccggacctg acccggatcg acgcccgcgg
     6661 ccggtggttc ttcgctccgc ttaccttgac cttcgccttg ggctttttga cctgattgcc
     6721 gccgttgccg ttgccattgc cgttgccgtt gcctccacca tttccgctcg ggcaggtgcc
     6781 ggtctcggtc gtcggcaggg ttcggctcac ggtctggccg gcctgggaag tgaagtcggc
     6841 attgaaggtc gaagattcgg cgcagacatc cccgatcacc ctgatcaggc cggtcggccc
     6901 gccggggaag accagctcga aacgcgagag gggcaggtcc ggcagaccct cgaagatcgt
     6961 ctccacgcgg ccgttcctga agccggaaga gccgaggatc ttcagcggga gcgtgccctt
     7021 caggtcaacg ccgagctggg gcagtccgtc ggcgcctacc gcgtagacgg ggccggacag
     7081 atcagaatcg agcagcgggc tgaccgccgt cgcttcaccg accttggtgg attcggggca
     7141 gtttccggcc tggaagtcag cgagcgggca ggtctggtcg aagcggccac catcgactcc
     7201 aagcccggcc ggcagcacca cgtcggcacg ctggttgttc gcctcggatt cagtctgggt
     7261 gatcaccgaa gtcaccgtcg gccgcgagcc ctgggccagc ggtttgccgt ttccgtcgat
     7321 gtgcaggctg gcctgcgggt cataggactg ggccgcgcag ttggtcgatt cgaaggtggt
     7381 cgagccggtc accgccggat caccgtcggc catcgcccat gagcgggccg agaatcctgc
     7441 cgtcttggtg ccgcatgaag tcggattgcg catgaaggcg ccgtccagat cgagatcgac
     7501 cgagttgatc gcgatcccga ccgggagcag gttgagcacc gcagcgttct gcggcaggtc
     7561 ggtgaggatg gtgtcgagcc cgaagtcgga gcgcagcttg acctggacct gcatgacgat
     7621 cggcggcagg agcaaatggg tcgggtccgg cgggcggagg acgattccca gccgggcggg
     7681 ctcaccgacc tcgggagcaa cgttgtagac actgccgttg acggtcagcg gcaccagcga
     7741 catggcggtg agattgacag tagtcgtgcc gatctgggaa ccggccgggc agctgtcggc
     7801 gttcaactgg tcagcagtgc atttcgccac cgattgtggg tttccggaaa ggcccggagg
     7861 caggctgacc gtgaggtcac gcaactggtc ctcgggcgaa gtgatctcca ggtgggtgtg
     7921 gaagtccgcg tgtgctccgg catcggtgtt ggccggagcc gccaccagcc cgctcagccc
     7981 gaaagccgcc tgggcggtcg aggccagcgc aaacgacccg gccagcccaa gcatgagagc
     8041 cagcaggaat ctcccgtaca ccctcttccg catccctctc accattcgtc ccgtaatccc
     8101 ttcaagccgc gatcgatcca tttgacagcc aataccccaa acagccgaaa ctcaatcggg
     8161 gtcccacatg ccgcgcggta cccggccgga tctgaatgaa ccggggcaaa cggggtaacc
     8221 cttctggctg gacgcaatgc cgatggccga agaggacaga tcgatccgag tggcggcagt
     8281 tggagacatt cactgccgcg aggccaaccg tgaaccgctg gcccggagca tcgcggcgat
     8341 ttcggacgcg gacctgctgc tgatcgccgg ggacatcacg agtcacggca ccgtcgagga
     8401 ggcacagatc ctctgcgagg tgaccgccgg ggttgacttt cccgtgttcg ccgtgctcgg
     8461 caaccacgac cttcatgccg gtcaggcgaa ggagatcatc gagaccttga gggcgggcgg
     8521 catcgatctg ctcgacggga cttcagcgat cagcgagatc aagggcgtcg aggtcggggt
     8581 tgtcggcagc cggggcttcg tcggcgggtt cgcgccgcat cacctgcctg acttcggcga
     8641 accctcgcta cgcgccgtct acgaggagac ctcccgcgag gtttccgccc tggaggccgg
     8701 actgagtgaa gtggcgacct gccagatccg gctcgtcgtc cttcactact cgccgcttcg
     8761 ggcgacggtc gaaggcgagc ctccggagat cttcccgttc ctcggcagcg accggatggc
     8821 gaggccttta cttgagcacg ggccggatct ggtcgtccac ggccatgccc acgagggaac
     8881 gcacgaaggc agcatcggcg aggtcccggt gcgcaacgtg tcgctgccgg tgctcggggg
     8941 cgagatctgg atcaccgaac tgaacgcccc cgttcacggc ccgatcccct gacggcccgg
     9001 gccgatctgc cattcgacac cgccggggcc gggcagggcg agcggctgaa tgccgagccc
     9061 gtcgaccagc acgaagaagg cccgcgcgaa aggactcgag gaagtccgtt caaccagaga
     9121 cggccagtcg atctgctcgc ggagcgcccg ggcaatctgg atcagcgtga ggaagtcggc
     9181 ggcatgctcg tccattgcca gcaacttggt cgtgaggatg tcgtcgatgc acatcaccgg
     9241 caccaccatg ccgcccacgt tcagttcttc agcccgttcg atcacctgct cggtaatccg
     9301 catgccggcc ggctggaaga tcaaatcgat caggacgtcg ccgtcccagg ccttgaccag
     9361 ccattgctcg ggtggccttt cggtgcgaag gccggcgtcg gcgaatgcct ggatcgcccg
     9421 ttccgcgtcg gcgggcctga ccatcaggtc gatgtcgttg ctggaaggcg gccccccgtg
     9481 cgcccaggtc ccgaggcccc ctccgaggag gtagggaatg ctttcgcgct ccatgatcgc
     9541 tgccgccgac ttcaggctgg tggtcaggat ctcggtcttg tcggggtcgc tctctttcat
     9601 gccgcaaccc gccgggtacc cccgtcaagc gaggagaaaa cggtccggtg cgccgccttc
     9661 ccgggcggac tttgccgcag cccgaatcgc tagaatcggc ggcgaattga tggatcgggt
     9721 tgggggctcg agttaattga tcaccggccg gctttggcgg accagtcgcg gggaaagccc
     9781 tgcgtggaac tctgcccggg ttcggtctgg agaagggaaa acagaaggat gaagtcagga
     9841 atcaccgtag gtctggcggc gctggtcgcg tgcgccgggc tcgtctcgtc agcgcatgct
     9901 gcctttccgg gcaagaacgg caggatcatc ttcgaacgca aggccagcca gttcgcgggc
     9961 aactcggatc cctgggtgtt ctcgggtggc aaggcgaggc ggctcgtcag gatcagcaac
    10021 aacgcttaca acttctccta ctcgccgaac ggcaagaaga tcgtcttcga ggcaaccgtg
    10081 ccctcggagg agcttgtcgt catgaacgcc aacggcacgc ggccgagggt gatcaccgcg
    10141 agggttcaca aatgcatcgg caagcggcac ccgacctggt ctccgaacgg caagaagatt
    10201 gccttcgtct gcctcaaccg gaacagcctc tccgatcacg acgtgtggtc ggtcaacgcc
    10261 aacggaaccg gcatccgccg gatttccagg acccacgatg cctattcccc ggcctggtcg
    10321 ccgcgcggaa acaagatcgc ctacacgacc tacggcgggt cgatctacac ggtcccggcc
    10381 cggggcggca agagcaagct gctcattgcg gaggctcccg gcggcgtgtt cggcggcacc
    10441 ttccagggcg tcgactggtc gccgaacggg cgccttctgg tctcggactc gagtggcgac
    10501 ggcgtctaca cgatcaacgc ccggaccggg gccacctccg gcgatctcgc agaaatcggg
    10561 gccgagccgg tcttctcgcc cgacgggaag aagatcctct acgtgggcat agcggagtcc
    10621 aggggaagca ccaggatcgg cctctggaag atgaacctcg acggctcaaa caagcagcag
    10681 gtcacccggg gcggctacga ccgggcaccc agctgggcac ccgcccgcta acggaatcgg
    10741 ggtcggttca gcctggaacc ggccccgttc gcactgcctg aggggtgagc gacggggatc
    10801 gaacccgcga ccgcctggac cacaaccagg agctctacca actgagctac gcccaccgcg
    10861 aggggaagaa atctaccggt cggctctcgg atacgctgcg ccggtgccgc agccagcaag
    10921 cccgggagat gcgcgatgag tgtgcgccgc gaaatgcggg gcttccttgt cggttcgctc
    10981 ctctttgccc tcggcgcgac tcccggctat ctcggactgg tcggcgtcac cgcggacaac
    11041 ctgacctact tcctcggctc gatcttcttc acgattgccg gcttcaccca gctgcggctg
    11101 accggccgct ggcaacgcgg cggctggtcg gaccgcgacg cctgggccga ctggtgggca
    11161 gcggcaatcc agtttctcgg cacccttgcc ttcaacgttt ccaccttggc agccctgctc
    11221 ggcacgatcg atcccggcac cttcgagcgc ctgggctggc gccccgactt catcggttcg
    11281 atctgcttcc tcgttgcctc ggtgctcgcg gtcgaggcga ccaccaaccg cgaatcgctg
    11341 tgggatccgg aagcccgcaa ctggtggaac acctggctga acatggccgg ctcggtcgcc
    11401 ttcatgatct cggcgatctt tgcctggatc aatccctcca ccggcgagcc gctgaatacg
    11461 gactgggcca accttggcac cttcttcgga gcgatcggct tcttcgccgc ggccgccctg
    11521 ctcaagcccc aggccgacgc cgactccaac ccttcaatga acggcaccgg ccccgccgaa
    11581 aacggggatt agggctcgat cggggccggg gcgcgggccg aggaaagcgc gccggccgag
    11641 gccgagacga ccagcgcgat cgccgcgatc tgggccgcgg acatgtcctg gccgaggagc
    11701 aggaagccga tcgtcgcggc cattgccggc tcgaggctca tcatcacccc gaagacgttc
    11761 gagggcaggc ggcggagcgc ctccatctcg agtgagagcg gcagggtcgt gctgaggatc
    11821 gccaccgcga agcccaccgc caggaccgaa ggcagcagca ggtccgcgcc gccgtcggca
    11881 accccgaagg gcgtgcagat gacggtcgag aagaccatcg cgatggccag tccctgcccg
    11941 ccgtagaact cctcccccac cctcttgccg agcaggatgt aacagcccca gaacacaccg
    12001 gccaccacgg cgaggacgac cccgaagagc tcaatgcctt cttcgccgat gccaccggaa
    12061 agcagcagga tcccggccgc cgccatcgcg acccagatca ggtcccggcg ccggtgggag
    12121 gtgatcaggg caaccccgag tggcccgatg aactcgaagg tcacggcgat gccgagcggg
    12181 atccggtcga tcgcctcgta gaaggagagg ttcatcgcag ccagcacgaa accgaagagc
    12241 agggcgatcc tggtccggcc ccggtccaga cggaagcttg gtcgccagat caggaccagc
    12301 agcaccgccg agatcagcgt cctgaggagg gttacgccca tcgccccgac ctcgtcgaag
    12361 agaccggtgg cgagcgccgc tccgacctgg atcgagctga tcgacccgag caccatcgcc
    12421 accgccggca gggacggcga gggatgaggt gatcggcttt cgttggaagc ggccatcagg
    12481 gcagtgaatc accggagaaa taaccgtgcc acgagccctc aggaataatc cccccatgac
    12541 cgacacgccg ccgacccggg acgggtatcc gcacttcctg acgatcccca cccgctggaa
    12601 ggacaacgac atctacggcc acgtcaacaa cgtcgagtac tactcgtact tcgacacggt
    12661 catcaacgag tatctgattc gcgagggcgg gctggacatc cacggcggcg ggacgatcgg
    12721 ggtctgcgcc gaatcccact gtcgcttcct ggccgcggtc gcctttcccg atccggtcga
    12781 agcaggcctg agggtcggcc atctcggcag ctcttcggtc cgctacgaga tcggcctctt
    12841 cgccgggaac ggcgaaccgc ttgccgaggg ctggttcgtc cacgttttcg ttcagcgcga
    12901 aagtcgccgc cccgacggca tcccggattc gatccgctgc gcactcgaga aactggaggt
    12961 ccattcg
//
LOCUS       DAOQKN010000055        12932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_2260_length_12932_cov_5.414227, whole genome shotgun sequence.
ACCESSION   DAOQKN010000055 DAOQKN010000000
VERSION     DAOQKN010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 12932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12932
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2260_length_12932_cov_5.414227"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..1346
                     /gene="argJ"
                     /locus_tag="PLJ64_05325"
     CDS             159..1346
                     /gene="argJ"
                     /locus_tag="PLJ64_05325"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HNC92939.1"
                     /translation="MTGYPSLSVPPEGDFFRSRWVSPPAGIEFNEDPVLPDGFRAGGV
                     ACGLKGGGVTDLGAVVCDSGEVSSAILLTANAAAAAPIRVCRESLWQGEVRAAIVNSG
                     NANAATGEQGILDALSMRNSLATELGLAPQTVGVAETGVIGVPMDMEAVISGIGGLAE
                     SLSAEGAVSFSEAIMTTDKWPKRVSLSVDGITLSAQAKGAGMIEPGFATMLCFVQTDA
                     VVPEPEAALRQAVSGSFERITVDGQMSTNDTVLLQASGSSGRELPAGLLDAVLLQLAL
                     EIVSDGEGASRVGRVEVTGAADQAEAELVARKIANSPLVKTALLGRDPNWGRIAQAAG
                     QALAGHDIGELGPEVIEAGDIAGGGREAELALRLDRGDGTAHVFTSDLTHEYIVINAE
                     YTT"
     gene            1370..2242
                     /gene="argB"
                     /locus_tag="PLJ64_05330"
     CDS             1370..2242
                     /gene="argB"
                     /locus_tag="PLJ64_05330"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HNC92940.1"
                     /translation="MDPKDIANPIIDDDSVNVLLEALPYIREFHGKTVVIKYGGAAMR
                     EESLRQAFADDVVLLKYVGLNPVIIHGGGPEITKYMNKMGIEVRFQHGLRVSDAETVE
                     IAKMVLLGKLNADLVARLGRAGSPAVGLSGEDGGLFDVAPVANAEEVGFVGDIERVDI
                     DVLNHISEDYIPVIASVGSDRDGNSYNINADEAAGKVAAALGAFKVVFLTDIVGWLSD
                     PEDESSRISETTVAEVEDALESISGGMRPKLTACVDAIRGGAQNAYIIDGRRPHSLLL
                     ELFTDAGIGTMVTP"
     gene            2239..3435
                     /locus_tag="PLJ64_05335"
     CDS             2239..3435
                     /locus_tag="PLJ64_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNC92941.1"
                     /translation="MNEQVRADEAKYVMQTYKRAPVEFVRGEGALLFDGEGRQYIDFL
                     GGISVASVGHCHPAVVEAIRQQAGELLHVSNLFYTAPMVELAKRLSESSLGGKVFFCN
                     SGTEANECAIKIARKHARSRGVESPEIVSFESDFHGRSYGALSATPGLAANPDFAPML
                     PGFRSVPFDDAAALRDAVGPDTAAVLIEPIQGEAGVFPLSDNTLLAAREACDEAGALL
                     IFDEVQTGIGRTGSLWAYEQGPVRPDVLTTAKALGGGTAIGACIGNPETGEVLAAGDH
                     GSTFAAGPVASSAALAVLDLVDDPEMLRRIRALGASLTERLNALDGVAEVRGRGLMIG
                     VRLEPGLNAGEVNAALLGNGLVANAPRPDTLRFLPPFVLTDEQADRAVALIDETLAGY
                     HPDESN"
     gene            3449..3931
                     /locus_tag="PLJ64_05340"
     CDS             3449..3931
                     /locus_tag="PLJ64_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92942.1"
                     /translation="MSSDDRTRVMRNDEPPRRRHDEDPHYTPEEQKKHLRNLIIVLIA
                     VIVGLVIAVIVISSGGDSSDDTSATTGDTAVTTPVVPVTPSGETGTTDSSGEDDGGVT
                     PTDTGTDTSGGISPDTSTGTGTDTGGVTPDTSTGTDTGGVTPDTSTDSGSSSGGIGPG
                     "
     gene            3928..5199
                     /locus_tag="PLJ64_05345"
     CDS             3928..5199
                     /locus_tag="PLJ64_05345"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNC92943.1"
                     /translation="MTEISLDSLSGPVASYEARPEEVNRVVLLYSGGLDTSVMLHWIQ
                     QAYDAEVVTLTVNLGQPGEDYGAILDKAKAMGAVETVVIDAREEFAEDFVLPAIKANA
                     LYGGGYPLFTSLGRPLIGKLGVEVAAEYACDTLAHGCTGKGNDQVRLEGTVATLNPDL
                     KMIAPVRAWQIGREEELEYCRQNGIPLKGGTSESPYSIDDNLWGRSSEGKVIEQLDTA
                     PPDDVFQLVTRPEEAPDEPELVTIGFEAGRPVSFNGERLDLVPLLEAAAEAGCRHGCG
                     IVDHIEDRIVGLKVRDIYEVPAAALVLTAHQDLEKVVSTIHQNNFKHSLDRHWAYMVY
                     AGLWYEPLRHDLDSYMDSANEFITGEVTLKLYKGSVTPVARKSDYAVYDENLASFGAS
                     GGEFSQNAAPGFIELFSLQSRMAHQVRNRKE"
     assembly_gap    5358..5457
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5458..6481
                     /locus_tag="PLJ64_05350"
     CDS             <5458..6481
                     /locus_tag="PLJ64_05350"
                     /inference="COORDINATES: protein motif:HMM:NF018447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HNC92944.1"
                     /translation="LRILAPTRTVFTALTGGLALALACALLLISLNSGAASAASLEQK
                     QAAARDRIQQTSDQIAAGRADLDAAQARADAAAAREADLSGLLASGEARSAELADRLA
                     AAEADLARSKKRLDRARKYLARHLVAVYMHGGEPDTLDMALGAGSFDQLTTGTEYLTA
                     IEDTNERLMDRVAELRRELDGKVDGLGDAKQAIDEHNSALAAARDQIASVQAAAASSA
                     ATLAAENATRAGQITSLKGDIAGWQKQIEKQQQVSAEQAQVEVEQTLGGPYSIPTYIV
                     MCESGGDYSAVNPSSGAGGAYQIMPSTWEAYGGTGLPQDASKAEQDRIAALIWADSGS
                     SPWTCG"
     gene            complement(6544..7002)
                     /locus_tag="PLJ64_05355"
     CDS             complement(6544..7002)
                     /locus_tag="PLJ64_05355"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNC92945.1"
                     /translation="MIYLLRHGDAEPDQGSGDAARRLTEKGEAQALAAGAAMAKLGMT
                     VDTCLASPRVRAFDTATLACRSLGIKVEVSEPIGYGDYDSLELAAGRGDTLIVGHEPV
                     LSMEVARLTGANIKMKKGGLALIKGSHLHALLRPTELIEIATGGARSSNI"
     gene            7042..7839
                     /locus_tag="PLJ64_05360"
     CDS             7042..7839
                     /locus_tag="PLJ64_05360"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNC92946.1"
                     /translation="MTEAAAVPDFSTLLVSAEGPIGRLTLNRPEKLNALSGELLNELI
                     LASKWFTDRTGVKVVLVSGAGRAFCAGADLTSFSAAGSDPSEEERRDGADLGRRMADA
                     VTGMRPMTVAAIQGHCVGGGLVLAAACDLRIAAEDTRFSIPEVDLGIPLAWGGIPRLV
                     REIGPAATKELVLTCREFDAREARELGFLNRTVDPDRLEGQAWELAEKLASQPAYSLE
                     LTKRHVNAVAEESGSTADAITDADGLLGALGDPESLRKMGEYLARRG"
     gene            7945..11472
                     /gene="dnaE"
                     /locus_tag="PLJ64_05365"
     CDS             7945..11472
                     /gene="dnaE"
                     /locus_tag="PLJ64_05365"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNC92947.1"
                     /translation="MSSSASSHGQVVHLHVHSEHSVLDGACNIKEMAARAAELEMPAL
                     ALTDHGVMNGALDMYKACRANDIEPILGIEAYYVDDRIAVKETAGRYERNHLTLLAEN
                     NTGFANLVALSSEGFLEGYHRGKANVDMELLERHSEGVIALTGCLQARFVKRLVEDRP
                     DEAREHLDQLIQVFGPEQVYLEVQKNGLADQNKANEQIARIAREVGRPLVGTADVHYL
                     HREDYNNHTALLCVQTKSTLDDPKMSFDTNEFYLKDSREMHEAFAEWPEAIPSTIEIA
                     ERTDIEIPLGRILLPSFPTGNGESESQMLRRLVEEGLKDRYGDPVPAEARERMEFELG
                     VIEEMGFPAYFLIVWDFIHYAKENGISVGPGRGSAAGSIVSYSLGITDLDPVEHDLLF
                     ERFLNPGRKSMPDIDIDFSVRGRERMIQYVTNKYGSDSVAQIITFGKMAPRAAVRDAA
                     RVHGFDYATGDRLAKEIPEPIMGRSPSFEECLRDGEDLRKTYDSDPDAAKIIDTARGL
                     EGKIRNTSIHAAAVVISGRPLQEIVPLQLVEDKSGQAVTGEDGKPVKQFKTVTQYSMG
                     PVEEIGLLKMDFLGLRNLDVIDDAIDIIKRSHGVEIDISEIPLDDPVTYEMLARGDSV
                     GVFQLESDGMREAMRRVVPTEFDDITALVSLYRPGAMKEIPRYAEGKHDPESVEYSDP
                     RLKEITESTYGCFLYQEQLMAVAKQMAGFEPSEADDLRKAIGKKKREMMAAMKPKFME
                     GLKASGTDHALATYLWSINEAAADYSFNKSHAACYALISYRTAYLKANYPAEYMAAVI
                     SSVMSTKDKVPFFVNHCSSMGIRVLPPDVNISDHSFVVDEGNIRFGLDAVKNVGYSAV
                     DAIINARNDRPFESLWDFCERVDNRTVNKRAVECLIKCGAFDLLPGSRQGMLEKLPDA
                     AAAGAKAQSDLMLGQESIFSLGGADEGAGGGSHEPISEVEFDQKELLALEKETMGTFL
                     SSHPLDGLKEAILAEADCSLGDLRLQEDGSRVKVGGLITAYKKVRTRAGKNIAFATLS
                     DIEHDIELMLFNLEDSEKQQLVQMDEVVMVRGTVDQSEEGSKLKVRDAELFNPSEAQI
                     EKAKIAYAKKIEPFVVRVVPEQMSLDTLEDMKSVFGQHQGESDVVVVVEGDREHRLKL
                     GPDFRVRHSPGLATEIDQIFGDGTAARAA"
     gene            11614..12876
                     /locus_tag="PLJ64_05370"
     CDS             11614..12876
                     /locus_tag="PLJ64_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNC92948.1"
                     /translation="MTATETSEETTVDESSEPKFSLELNQDQKDIQEWVHGFAENVMR
                     PAAHEWDEREEFPWEIVQEAAKIGLYGFEGTAQFFADPTGLSLPIVNEELFWGDAGLG
                     MAIQGTGLAVASIFGQGTPEQMGEWVPQCYGTEDDVKIAAFCSSEPDAGSDVSNVRTT
                     AKYDEAKDEWVINGQKAWATNGGIANIHVVIASVDRELGSRGHAAFIIPPNTPGCSQG
                     AKVKKHGIRASHTADVHLDDCRVPGSCLLGGKEKLDERLARVREGKKGKSQAAMATFE
                     ASRPIVGSQAIGIARAAYEYALEYAKERVQFGRPIIENQAIAFTLADMCLEIDAARLL
                     VWRASWMGRNQIPFKNSEGSMSKLKGGEVATWVTERAIQILGGNGYTREFPVERMHRD
                     AKIYDIFEGTAEIQRLVISRSISGIKIK"
BASE COUNT         2383 a         4170 c         4212 g         2067 t
ORIGIN      
        1 gacgtgcgcg agaccaattt ctgcgacatc ttcccgctgg tcaccgatga ccggatcctg
       61 gtcgaatcgg cgatcgacaa cctctggaag ggcgcctcct cgcaggcgat ccagaacctg
      121 aacctgatgc tcggtctcga cgaaaccgag gggatcggat gaccggctac ccgagcctca
      181 gcgttccgcc ggagggggat ttcttccgct cccgctgggt gagcccgccg gccgggatcg
      241 agttcaacga agacccggtg ctgcctgacg gcttccgggc aggaggggtc gcctgcggcc
      301 tgaagggcgg cggggtgacc gacctgggag ccgtcgtctg cgattccggg gaggtcagct
      361 cggcgatcct gctgaccgcc aatgcggcgg ccgcggcgcc gatccgggtc tgccgggaaa
      421 gcctctggca aggcgaggtc cgggcggcga tcgtgaattc cggcaacgcc aacgccgcca
      481 ccggcgagca ggggattctc gacgcgcttt cgatgcgcaa ctcgctggcc accgagctgg
      541 ggctggcccc gcagaccgtg ggggtcgccg agaccggggt gatcggcgtg ccgatggaca
      601 tggaagcggt gatctccggc atcggcggcc tcgccgaatc gctctcggcc gaaggggcgg
      661 tcagtttctc ggaggcgatc atgaccaccg acaagtggcc gaagcgggtt tcgctgagcg
      721 ttgacggaat caccctctcc gcccaggcga agggcgccgg gatgatcgaa cccggcttcg
      781 ccaccatgct ctgcttcgtc cagaccgacg ccgtggttcc cgaaccggag gcggcgctca
      841 ggcaggccgt gagcggctca ttcgagcgga tcacggtcga tggccagatg agcacgaatg
      901 acacggtgct gcttcaggcc agcggcagct ccggccggga gcttccagct ggtctgctcg
      961 acgcggtgct gcttcagctc gcgctggaaa tcgtctccga tggcgaaggc gccagccggg
     1021 tcggtcgggt cgaggtgacc ggggcggccg atcaggccga ggccgagctg gtcgcgcgca
     1081 agatcgccaa ctcgcctctg gtcaagaccg ccctgctcgg tcgtgatccg aactgggggc
     1141 ggatcgccca ggccgccggc caggccctgg ccggacatga catcggcgag cttggccccg
     1201 aggtgatcga ggccggggac atcgccggag ggggccgcga agcggaactg gccctgcggc
     1261 tggatcgcgg cgacggcacg gcgcatgttt tcaccagcga cctgacccac gagtacatcg
     1321 tgatcaacgc ggaatacacg acgtagtaca ggcagacaag gaaagagcaa tggatccgaa
     1381 ggacatcgca aacccgatca tcgatgacga ctccgtgaac gtgctgctcg aggccctgcc
     1441 ttacattcgc gagttccacg gcaagaccgt ggtgatcaag tacggcgggg ccgcgatgcg
     1501 cgaagagtcg ctgcgacagg cgtttgccga tgacgtggtc ctgctcaagt acgtcggcct
     1561 caatcccgtg atcatccacg gaggcgggcc cgagatcacc aaatacatga acaagatggg
     1621 catcgaggtc cgcttccagc acggccttag ggtctctgac gcggaaacgg tcgagatcgc
     1681 gaagatggtg ctcctcggca agctcaacgc cgatctcgtg gcccgactcg gccgggccgg
     1741 ctcgcctgcc gtgggacttt ccggtgaaga cggcggcctc ttcgatgtcg cgccggtagc
     1801 caacgccgag gaagtcggat tcgtcggtga catcgagcga gttgacatcg acgtgctcaa
     1861 ccacatcagc gaggattaca tcccggtgat cgcctcggtc ggatccgacc gggatggcaa
     1921 ctcgtacaac atcaatgccg atgaggccgc cggcaaggtg gcggcggcgc tcggcgcctt
     1981 caaggtcgtt ttcctgaccg acatcgtcgg ctggctgagc gatccggagg atgagagttc
     2041 gcggatctcc gagaccaccg tggccgaagt cgaggatgcc ctggagtcga tctccggcgg
     2101 catgcgaccc aagctgaccg cctgcgtgga cgcgatccgc ggcggcgccc agaacgccta
     2161 catcatcgac ggccgccgtc cccattcgct gctgctcgaa ctcttcaccg acgccggcat
     2221 cggcaccatg gtgaccccgt gaacgagcag gtccgggccg acgaggccaa gtacgtaatg
     2281 cagacctaca aacgggcccc ggtcgagttc gtccggggcg aaggcgcgct gctcttcgac
     2341 ggggaaggcc gtcagtacat cgacttcctc gggggcatct cggtcgcctc ggtcggccac
     2401 tgccacccgg cggtcgtgga ggcgatccgg cagcaggccg gagaactcct tcacgtctcg
     2461 aacctcttct acaccgcccc gatggtcgag ctggccaaac ggctctcgga gagctcgctg
     2521 ggcggcaagg tcttcttctg caactccggc accgaagcca acgaatgcgc gatcaagatc
     2581 gcccggaagc acgcccggag ccgcggggtc gaaagcccgg aaatcgtctc gtttgaaagc
     2641 gacttccatg gccgcagtta cggtgccctt tcagccacgc cgggcctggc cgccaacccg
     2701 gacttcgcgc cgatgctgcc gggcttccgc tcggttccgt tcgacgatgc cgcggcgctt
     2761 cgtgacgccg ttggtccgga caccgccgcg gtgctgatcg aaccgatcca gggcgaagcc
     2821 ggcgtcttcc cgctttccga caacaccctg ctcgccgccc gcgaagcctg cgacgaggcc
     2881 ggggcgctgc tgatcttcga cgaggtccag accgggatcg gccggaccgg ctcgctctgg
     2941 gcctacgagc aggggccggt gcgaccggat gtcctgacca cggccaaggc gctcggcggg
     3001 ggcacggcga tcggcgcctg catcggcaac cccgagaccg gggaggtgct ggctgccggc
     3061 gatcacggct cgaccttcgc ggccggaccg gtcgcttcca gcgccgcgct cgccgtcctc
     3121 gacctggtcg acgaccccga gatgctgcgg cggatccgcg cgctcggcgc ctccctgacg
     3181 gaaaggctga acgcccttga cggggtggcg gaagtgcggg ggaggggctt gatgatcggg
     3241 gtccggctgg aacccggttt gaatgccggc gaggtcaatg cggccctgct cgggaacggg
     3301 ctggtggcca acgcgccgcg acccgacacc cttcgctttc tgccgccgtt tgtccttacc
     3361 gatgagcagg ctgaccgggc tgtcgccctt atagacgaga cgctggccgg gtaccatccg
     3421 gacgaatcga attgactgga gatttccgat gagtagcgat gaccgcacaa gagtgatgag
     3481 aaacgacgag ccgccccgcc ggcgccacga cgaggaccct cactacacgc ccgaagagca
     3541 gaagaagcac ctgcgaaacc tgatcatcgt gctgatcgcc gtgatcgtgg gcctggtgat
     3601 tgcggtgatc gtgatcagtt ccggcggcga cagcagcgac gacacttcgg caaccaccgg
     3661 agacactgcc gtgaccacac cggtcgtccc ggtcaccccg agtggagaaa ccggcaccac
     3721 cgattcctcc ggtgaagatg acggcggggt caccccgacc gacaccggca cggacacttc
     3781 cggcggcatc tcgcccgaca ccagcaccgg taccggcacg gataccggcg gtgtcactcc
     3841 cgataccagc accggcaccg acaccggcgg agtcaccccg gacacgagca ccgacagcgg
     3901 ctcaagcagc ggcgggatcg gccccggatg accgaaatct cactcgattc gctttcgggc
     3961 ccggtcgctt catacgaagc ccgccccgaa gaggtcaacc gtgtcgtgct gctctattcg
     4021 ggtggtctcg acaccagtgt gatgctccat tggatccagc aggcctacga cgccgaggtg
     4081 gtcacgctga ccgtcaacct ggggcagccg ggtgaggatt acggtgcgat cctcgacaag
     4141 gcgaaggcga tgggggcggt cgaaaccgtc gtgatcgacg cccgcgagga attcgccgag
     4201 gacttcgtgc tgccggcgat caaggccaat gccctttacg gcggcggcta cccgctcttc
     4261 acctcgctcg gccggccgct gatcggcaag ctcggagtcg aggtcgcggc agagtacgcc
     4321 tgcgacaccc tcgctcacgg ctgcaccggc aagggcaacg accaggtccg gctcgaagga
     4381 acggtggcga ccctgaaccc cgacctcaag atgatcgccc cggtccgggc ctggcagatc
     4441 ggacgcgagg aagaactcga gtactgccgc cagaacggca tcccgctgaa gggcggcacc
     4501 tccgaatcgc cctactcgat cgacgacaac ctctggggcc gctcctcgga gggcaaggtc
     4561 atcgagcagc tcgacaccgc tccgccggat gacgtcttcc agctggtcac ccggccggaa
     4621 gaggctcccg acgagccgga actggtgaca atcggattcg aagcgggtcg tccggtcagc
     4681 ttcaacggcg agcggctcga cctcgtgccg ctgctcgagg cggcagccga ggccggttgc
     4741 cgtcacggtt gtggaatcgt cgatcacatc gaggaccgca tcgtcggcct caaggtgcgc
     4801 gacatctacg aggtcccggc ggcggcgctc gttctgaccg cccaccagga tctcgagaag
     4861 gtcgtttcga ccattcacca gaacaacttc aagcattcgc tcgaccggca ctgggcctac
     4921 atggtctacg ccgggctctg gtacgaaccg ctgcgccacg atctcgattc ctacatggat
     4981 tcagccaacg aattcatcac cggcgaagtc accctcaagc tctacaaggg gtcggtgact
     5041 ccggtcgccc gcaagtccga ttacgcggtc tacgacgaga acctcgcttc cttcggggca
     5101 tccggcggag agttctcgca gaacgccgcc ccgggcttca tcgagctctt ttcgcttcaa
     5161 agccgcatgg ctcaccaggt ccggaaccgg aaggagtaga ggttcccgcc gggcaagccg
     5221 gaggcccgtg ttccggcaga cggttctagg gtaaattcag gacatggagg caatcaggtg
     5281 atctacaagc tgatcggaat ggccgtcgtc aaactcggct ggatgttcct ccgccgcaag
     5341 gccgccgcca accgcggnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncct
     5461 tcgcattttg gctccaactc gaacagtttt caccgccctg acgggtggtc ttgcgctggc
     5521 gctcgcctgc gcgctcctgc tcatatccct gaattcaggg gcggcttcgg ctgcctctct
     5581 cgagcagaag caggcggctg cccgcgaccg gatccagcaa acttcggatc agattgcggc
     5641 cggtcgcgcc gacctcgatg ccgcccaggc ccgggccgat gcggctgccg cccgcgaagc
     5701 cgacctttcc ggcctgctcg ccagcggcga ggcccgatcg gcggaactgg ctgaccggct
     5761 ggccgcagcc gaagcggacc tggcgcgctc gaagaaacga ctggaccggg cccgcaagta
     5821 cctggcccgg cacctggtcg ccgtctacat gcacggcggc gagccggaca cgctcgacat
     5881 ggcccttggc gccggctcat tcgaccagtt gaccaccggc accgagtacc tgaccgcgat
     5941 cgaggacacg aacgagcgct tgatggaccg ggtcgccgag ttgcgccgcg agctcgacgg
     6001 caaggtggac ggcctcggtg acgccaaaca ggcgatcgac gagcacaact ccgccctcgc
     6061 cgccgcccgc gaccagatcg cctcggttca ggcggccgcc gcatccagcg ccgcgaccct
     6121 tgccgccgag aacgccaccc gggccgggca gatcacttcg ctcaagggtg acatcgccgg
     6181 ctggcagaag cagatcgaaa agcagcagca ggtaagcgcc gaacaggccc aggtagaggt
     6241 cgagcagacc ctcggcggcc cgtactcgat cccgacctac atcgtgatgt gtgaatccgg
     6301 cggtgattac tcggcggtca acccctcaag cggggctggc ggggcctacc agatcatgcc
     6361 ttccacctgg gaggcctacg gtggcaccgg actgccccag gatgcctcga aggccgaaca
     6421 ggaccgcatc gccgccctga tctgggccga ctccggctcc agcccctgga cctgcggctg
     6481 acttaactgc aagtcgggcg gggtcggacc cgccctccaa gttcaacccg cccgatcaga
     6541 tcatcaaata tttgatgatc gggctccgcc ggtggctatt tcgatgagtt ccgtggggcg
     6601 gaggagggcg tggaggtggc ttcccttgat cagggcgagg ccgccctttt tcatcttgat
     6661 gttggctccg gtgaggcggg ccacctccat cgagaggacc ggttcgtggc cgacgatcag
     6721 ggtgtcgccg cggccggcgg caagctcgag gctgtcgtag tcgccgtagc cgatcggctc
     6781 cgagacctcg accttgattc ccagcgaccg gcaggcaagg gtggcggtgt caaaggcgcg
     6841 gacccggggc gaggccaggc aggtatccac ggtcatgccc agcttcgcca tcgccgcgcc
     6901 ggcggccagg gcctgggcct cgcccttttc ggtgaggcgg cgggcggcat ctcccgaacc
     6961 ctggtcgggt tcggcgtcac cgtgacgaag caggtagatc atgggtcgag attacccgcc
     7021 aactgcaact agaatcggcc catgaccgag gccgccgcag ttcctgactt ctccaccttg
     7081 ctggttagcg ccgaggggcc gatcgggagg ttgaccctga atcgtcccga aaagctcaac
     7141 gcgctttccg gtgaactgct gaacgagctg atcctggcct cgaaatggtt cacggacaga
     7201 accggggtga aggttgtcct ggtcagcggt gccgggcggg ccttctgcgc cggggccgac
     7261 ctgacctcct tctcggccgc cggttcggac ccgagcgagg aagaacgccg ggacggggcg
     7321 gacctggggc ggaggatggc cgacgccgtg accgggatgc ggccgatgac cgtcgccgcg
     7381 atccaggggc actgcgtcgg gggaggcctg gtgctggccg ccgcctgcga tttgcgcatt
     7441 gccgcggagg acacgcgctt ttcgatcccg gaagtggacc tcggcatccc gctcgcctgg
     7501 ggcggcatcc cccggctggt cagggagatc gggccggccg caaccaagga actggtgctg
     7561 acctgccgcg agttcgatgc ccgggaagcc agggaactcg gctttctcaa ccggacggtt
     7621 gacccggacc ggctcgaagg ccaggcctgg gaactggcgg agaagctcgc ctcccagccg
     7681 gcctactcgc ttgaactgac caagcggcac gtgaacgccg tggctgaaga atccggttcg
     7741 acggccgatg cgatcaccga cgccgacggc ctgctcggag ccctcggcga ccccgaatcg
     7801 ctccggaaga tgggcgaata cctggcccgt cgcggctgaa cgccagcggg gatcgggcct
     7861 tcccgcaagt tacgggaaaa agcgtcccga gcggagcaaa tcgaccgggt ttgcatcatg
     7921 cgcacttgcc atcatggacc ggcgatgagt tcttcggcat ccagccacgg acaggtagtc
     7981 caccttcacg ttcacagcga gcattcggtg ctggatggtg cctgcaacat caaggaaatg
     8041 gcggcgcggg cagctgaact ggagatgccg gctctggccc tgaccgacca cggcgtcatg
     8101 aacggcgccc tcgacatgta caaggcctgc cgggccaatg acatcgagcc gatcctcggc
     8161 atcgaggcct attacgtcga tgaccggatc gcggtgaagg aaacggccgg ccggtacgaa
     8221 cgaaatcacc tcaccctgct ggccgagaac aacaccggtt tcgccaacct ggttgcgctt
     8281 tcctcggagg gcttcctcga gggctatcac cgcggcaagg cgaacgtgga catggaactg
     8341 ctcgaacggc actccgaggg agtcatcgcc ctgaccggct gcctgcaggc ccggttcgtg
     8401 aagcggctgg tcgaggaccg gccggacgag gcccgcgagc acctggacca gctgattcag
     8461 gtgttcggac ccgagcaggt ctatctggag gttcagaaga acggtctggc cgaccagaac
     8521 aaggcgaacg agcagatcgc ccggattgcc cgggaggtcg gccggcccct ggtcggtacc
     8581 gccgacgtcc attacctaca ccgcgaggac tacaacaacc acaccgcgct gctctgcgtc
     8641 cagaccaagt cgacgcttga cgatccgaag atgagcttcg acacgaacga gttctacctg
     8701 aaggattcgc gggaaatgca cgaggccttc gccgagtggc cggaggcgat tccctcgact
     8761 atcgagatcg ccgaacgcac cgacatcgag attccgctcg gccggatcct gctgccttcc
     8821 ttcccgaccg ggaacggtga gtcggagagc cagatgctcc ggcgcctggt cgaggagggg
     8881 ctgaaggatc gctacgggga tccggtcccg gccgaagcgc gggaacggat ggagttcgag
     8941 ctcggggtga tcgaggagat gggtttcccg gcctacttcc tgatcgtctg ggacttcatt
     9001 cactacgcca aggagaacgg aatctcggtc ggtcccggtc gtggctcggc cgccggttcg
     9061 atcgtctcct attcgctggg catcaccgat ctcgacccgg tcgagcatga cctgctcttc
     9121 gagcgcttcc tgaaccccgg gcgcaagtcg atgcccgaca tcgacatcga cttttcggtc
     9181 cgtggccgcg aacggatgat ccagtacgtg accaacaagt acgggtcgga ttcggtcgcc
     9241 cagatcatca ccttcggcaa gatggccccg cgtgcggcgg tccgggatgc ggcccgggtg
     9301 cacgggttcg actatgcgac cggtgaccgg ctcgccaagg agatcccgga accgatcatg
     9361 ggtcgctcgc cctccttcga ggaatgcctg cgggacgggg aggatctgcg aaagacctat
     9421 gactccgatc ccgacgcggc gaagatcatc gacacggccc gcggccttga aggcaagatc
     9481 cgcaacacct cgatccacgc cgccgcggtg gtgatctccg gccgaccgct gcaggagatc
     9541 gtgccgctgc agctggtcga agacaagtcg ggtcaggcgg tgaccggtga ggacggcaag
     9601 ccggtcaagc agttcaagac ggtgacccag tactcgatgg ggccggtcga ggagattggt
     9661 ctcctgaaga tggacttcct cgggctgcgc aacctcgacg tgatcgatga cgcgatcgac
     9721 atcatcaagc gttcgcatgg ggtggagatc gacatcagcg agatcccgct cgatgatccg
     9781 gtcacttacg agatgctggc ccgcggcgat tcggtcgggg tgtttcagct tgagtcggac
     9841 ggcatgcggg aggcgatgcg gcgggttgtt ccgaccgagt tcgatgacat caccgcgctg
     9901 gtgtcgctct accgccccgg cgcgatgaag gagatcccca ggtacgcgga gggcaagcat
     9961 gatccggaat cggtcgagta ttcagacccc cggctgaagg agatcaccga atcgacctat
    10021 ggctgcttcc tctaccagga gcagctgatg gcggtcgcca agcagatggc cggcttcgaa
    10081 ccatccgaag cggatgacct gcgcaaggca atcggcaaga agaagcgcga gatgatggcc
    10141 gcgatgaagc cgaagttcat ggaggggctg aaggcttccg gcaccgacca tgccctcgcc
    10201 acctacctct ggagcatcaa cgaagccgcc gccgactact ccttcaacaa gtcccatgct
    10261 gcctgttacg cgctgatctc ctaccgcacc gcgtacctga aggcgaacta cccggccgag
    10321 tacatggccg ccgtgatctc ctcggtgatg agcaccaagg acaaggtgcc gttcttcgtc
    10381 aaccactgct cctcgatggg catccgggtg ctgccgccgg acgtgaacat ctccgaccac
    10441 agcttcgtgg tcgacgaggg caacattcgt ttcggcctcg acgcggtcaa gaatgtcggc
    10501 tactcggcgg ttgacgcgat catcaatgcc cgcaatgacc ggcccttcga atcgctttgg
    10561 gatttctgcg aacgggtcga caaccgcacg gtcaacaaac gggcggtcga atgcctgatc
    10621 aagtgcggcg ccttcgacct gctgcccggc tcccgccagg gcatgctgga gaagctgccc
    10681 gacgcggcgg cggccggcgc caaggcccag tccgacctga tgctggggca ggaatcgatt
    10741 ttcagtctgg gaggggccga cgaaggagca ggcggaggca gtcacgagcc gatcagcgag
    10801 gtcgagttcg accagaagga gctgctcgcc ctggaaaagg aaacgatggg caccttcctt
    10861 tcatcccatc cgctcgacgg gctcaaagag gcgatcctgg ctgaagcgga ctgcagcctc
    10921 ggcgatctga ggcttcagga ggacggctcc cgggtcaagg taggtggcct gatcaccgcc
    10981 tacaagaagg tgaggacccg ggccggcaag aacatcgcct tcgccaccct cagcgacatc
    11041 gagcacgaca tcgaactgat gctcttcaac ctggaggatt ccgagaagca gcaactggtc
    11101 cagatggacg aggtcgtgat ggtcaggggg accgtcgacc agtccgagga gggctcgaag
    11161 ctgaaggtgc gcgacgccga gcttttcaat cccagcgagg cccagatcga gaaggcgaag
    11221 atcgcctacg caaagaagat cgagcccttc gtggtccggg tggtgccgga gcagatgtcg
    11281 ctggacacgc tggaggacat gaaatcggtt ttcggccagc atcagggtga atcggatgtg
    11341 gtcgtggtgg tcgaagggga ccgggagcac cggctcaagc tcggtcccga cttccgggtc
    11401 aggcattccc ccgggctggc gaccgagatc gaccagatct tcggtgacgg caccgcggcc
    11461 cgcgctgcct gaacgacatt ccggcggacc ctttgccgac agagcccctt ctcgccatca
    11521 tttgagctag gagaaggggc gggtttccga ggtcgtcccg cgaagacggg acgacatgcg
    11581 aatgattaca ccgatccgtg taacattcgg ggtatgactg ctacagaaac ttcagaagaa
    11641 accactgttg atgagtcttc tgagcccaag ttcagccttg agctgaatca ggaccagaag
    11701 gacattcagg agtgggtcca cggctttgcc gagaatgtga tgcgccccgc cgcccacgaa
    11761 tgggacgagc gcgaggagtt cccctgggag atcgtgcagg aggccgccaa gatcggcctc
    11821 tacggcttcg agggcaccgc acagttcttc gccgacccga ccggcctcag cctgccgatc
    11881 gtcaacgagg aactgttctg gggcgatgcc ggtctcggca tggcgatcca gggcaccggc
    11941 cttgccgtcg cctcgatctt cggccagggc accccggaac agatgggtga atgggtcccc
    12001 cagtgctacg gcaccgagga tgacgtcaag atcgccgcgt tctgctcctc cgagcccgac
    12061 gccggctccg acgtctccaa cgtccggacc acggccaagt acgacgaggc caaggacgaa
    12121 tgggtgatca acggccagaa ggcctgggcg accaacggcg gcatcgccaa cattcacgtc
    12181 gtgatcgcct cggtcgaccg cgaactcggt tcgcgcggcc atgctgcgtt catcattccg
    12241 ccgaacaccc cgggctgcag ccagggcgcg aaggtcaaga agcacggcat ccgcgcctcg
    12301 cacactgccg atgttcacct cgacgactgc cgtgtgccgg gcagctgcct gctcggcggc
    12361 aaggagaagc tcgacgagcg tctcgcccgg gttcgtgaag gcaagaaggg caagtcgcag
    12421 gcagcgatgg cgaccttcga ggcttcccgg ccgatcgtcg gctcgcaggc gatcggcatc
    12481 gcccgtgccg cctacgagta cgccctcgag tacgccaagg aacgcgtcca gttcggtcgt
    12541 ccgatcatcg agaaccaggc gatcgccttc accctcgccg acatgtgcct cgagatcgac
    12601 gcggcccggc tgctcgtctg gcgtgcctcc tggatgggtc gcaaccagat cccgttcaag
    12661 aactccgaag gctcgatgtc aaagctcaag ggtggcgagg tcgcgacctg ggtcaccgag
    12721 cgtgcgatcc agatcctcgg cggcaacggc tacacccgcg agttcccggt cgagcggatg
    12781 caccgcgacg ccaagatcta cgacatcttc gagggcaccg cggaaatcca gcgtctcgtg
    12841 atctcccgca gcatctcggg aatcaagatc aagtagggga aacgaggtgc cgcaggttta
    12901 cgaccccaga ggtcgcaaac ctgcggcacc tc
//
LOCUS       DAOQKN010000056        12827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_2289_length_12827_cov_5.493815, whole genome shotgun sequence.
ACCESSION   DAOQKN010000056 DAOQKN010000000
VERSION     DAOQKN010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 12827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12827
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2289_length_12827_cov_5.493815"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..862
                     /gene="rsmI"
                     /locus_tag="PLJ64_05375"
     CDS             35..862
                     /gene="rsmI"
                     /locus_tag="PLJ64_05375"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNC92949.1"
                     /translation="MPGRLTVCPTPIGNMDDVSKRVREALVMADYVACEDTRRTGRLL
                     ERLEIRPKPRLVSNHEGNEASRAVELVKRMERGDKIALVSDAGMPAVSDPGYRLIRAC
                     IDRGIEVEVLPGPSVIPVALVASGLPTDRWRFEGFLPKRAGEMERVLNSAETVIAFES
                     PRRISNSLRALSQLAPDRQAAVCREMTKLHEEVSRGTLTELAMRFQGEVKGEIVLVIG
                     PAAAAEHGQDIAFAVEALKRLVKSGARPRAAATVVASLTGTRANDLYKALTGREPRR"
     gene            859..1770
                     /locus_tag="PLJ64_05380"
     CDS             859..1770
                     /locus_tag="PLJ64_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92950.1"
                     /translation="MSAVGSRVAYATCEDRISDWEDDLLSADLLAERGVEVEFAVWND
                     PAVDWESFDLTVIRSTWDYTERLEEFLAWCESVGPGKLRNVPAMILWNTDKRYLAELD
                     GAGLPVPPTALVAPGSNPPPFGGKVVIKPVSGAGARDTGVFDEANEEGGLALLEKLAR
                     QDEIAMIQPYIPWIDERGETAVLFFGGRFAYALKKRAFLPESGVAPVRPGTTVAEGMF
                     AEDLMSLADASEAEVELGAKVVAWLARRFGSVPLYARIDMVSSPQGEPVLMEVEVTEP
                     SFYLGLTTGLETPGPELFAAAVEAGLG"
     gene            1808..3301
                     /gene="metG"
                     /locus_tag="PLJ64_05385"
     CDS             1808..3301
                     /gene="metG"
                     /locus_tag="PLJ64_05385"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNC92951.1"
                     /translation="MSFYVTTPIFYGNGEPHLGHAYSTMAADILARHMRQRGEDVFFL
                     TGTDEHGEPIAQAAEKQGLTPQELVDRSAQSFRDMAAKVDATNDFFIRTTDQGHIERV
                     QAVVDQVKENGFVYEGTYEGWYCPRCADFKNENEIGPGDTCLIHEIPLDRETEQNWFF
                     RLSAFQDELEKLYEENPGMIQPDFRRNEALAFIRGGLNDVSLSRSQISWGVPLSWDPD
                     QVMYVWFDALLNYYTALGYAREGEDLTGRFWPASWHIIAKDILKFHTVYWPAFLMAAG
                     IEVPQRIFIHGFLLMGDKKMSKSLGNVLDPFEVIEKFGADALRFYCFREVSFGQDGSI
                     STEGFETRYESELANDFGNLASRTIAMISRYRDGVVPEAGPDPLLLADFEGITDRVSA
                     LLDDAQIGQALEEIWVLVRRLNRYVEEAQPWVLAKDEADAERLDQVLYSLAEGLRVLT
                     LLLHPYLPASTDILLEALAEPDQGLAPFGSRPGGVKVEKIDPLFPKL"
     gene            3319..3747
                     /locus_tag="PLJ64_05390"
     CDS             3319..3747
                     /locus_tag="PLJ64_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92952.1"
                     /translation="MRKARSLTAGVAIAAIGLGLIGASVAPASAAARPLSVRFFTPDG
                     KQRLEVKRKVGIGVGCTKNCAVVVDMKLVMPNTKPIKARVKGGIKAFSAKSPYIVLNN
                     PALRYLKLTYPRSRFSIKVTATDISNGRKTVKRRVYGFRA"
     gene            3754..4518
                     /locus_tag="PLJ64_05395"
     CDS             3754..4518
                     /locus_tag="PLJ64_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNC92953.1"
                     /translation="MIDSHSHVSRCPESPEKVIGAAREAGVERILTVGLDEASNREQI
                     ALCEQFDGLFAAVGRHPNDADGFDDAAAADLEQPAAHPKVVAIGETGLDFFRDTAEPD
                     SQRLAFIKQADIAARLDLPLVIHCRDQQGRDDAVSEIFATLEEFPDLTVILHCFSAPD
                     WVERAAERDWYCSFAGNVTYPANEDLRTAAARVPADRIMVETDAPYLTPQSRRRDRNQ
                     PAFVVETAELVAGLRGIDYAELDELVTANAARVYGW"
     gene            4515..5282
                     /gene="rsmA"
                     /locus_tag="PLJ64_05400"
     CDS             4515..5282
                     /gene="rsmA"
                     /locus_tag="PLJ64_05400"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNC92954.1"
                     /translation="MKLGQNFLADPNLLDAIVRDAGLSPEDVVLEVGAGEGVLTERLA
                     EVASQVHVIELDRGLEPGLSGVIGRPNVNLVWADAVKYDLAGLDPAPTRMVANLPYAV
                     ATPVILRSAFELPSIESWSVMVQKEIGDRLRAGPGSKTYGAPSVLIQLTGEVRQVRKV
                     SRTVFKPQPRVDSAIIRIDRTSPGPDEATRRLVRAGFAHRRKAMARSVDLAIPGSLRA
                     VRDGLEAAGIDPGIRAEALSPAQFAKLSGMIDLAADD"
     gene            5275..6132
                     /locus_tag="PLJ64_05405"
     CDS             5275..6132
                     /locus_tag="PLJ64_05405"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92955.1"
                     /translation="MTELDLPLELNAPAKLNLCLYLGPRRKDGLHELTSIFEPLTLND
                     TIRVTEGGPAALVDQVICPGVPGENLVSQVIRMMRSEGWDPPLLKVEIEKRIPVQAGL
                     GGGSADAAAMLRLGVGEVEDLAGLALLLGADVPSQLNPKPLLVEGVGQKLTTLPAPAE
                     HWVVLLPFERGLSTRAVFDEADRMKLGRSQEEIDDISGRLWAVATNAVSPLAYPALLI
                     NDLEPAAISLKPEIGLGKERLEAAGAGFTGMTGTGPTVFGLFPDRESAEAAAAAVGPE
                     ALVCQGGVV"
     gene            6143..7618
                     /locus_tag="PLJ64_05410"
     CDS             6143..7618
                     /locus_tag="PLJ64_05410"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DedA family/phosphatase PAP2 family
                     protein"
                     /protein_id="HNC92956.1"
                     /translation="MIKVPETKKGKRLTLAGIAAAALVIFILYREFFPELDLQQLLDD
                     FANFLGAWTYLVVGLLAFLETGAFVGLIVPGETAMLIGGAVAGQGVINVFLLIAIAWL
                     MAFLGDSTSFWLGHRLGREFILKHGPKFGISAERYEQVEDYFEKHGGKTVLMGRFIGL
                     VRALSPFVAGSSGMRYRAFAPYSILGSGLQITLHILAGYFFARSIEAAAEYVGLAALV
                     IGTMIVVSVASYLAWKYLREPNNRARAVEWMEGRWYSAWLVRLARRYETQLRWIQDRF
                     TPGGTFGLEVTTLFAIIAVASFVLFAYAGILLDDGGPTPGDLRAFDFRDLIETGWLTS
                     IAKVVTVLGSQVVLLPLITVTAVILAVRQHWSEVLVLVLSSIAIAFGIDWIKDWVARP
                     RPDGGMIEVAGYAYPSGHAAHSIFYVWLSVTIAVRLRPDMARKAALITGGILLAALVG
                     LSRVYLGVHYLSDVFGGWALGALCFSFFAVWALLLGQLRKN"
     gene            7623..7871
                     /locus_tag="PLJ64_05415"
     CDS             7623..7871
                     /locus_tag="PLJ64_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92957.1"
                     /translation="MVLAVSTQIWYFGAAGLVSLAAFVGLILVPAVSSYGRWHERFAA
                     GFLSLLIFGGLITIGVIAGLAWVLISNGIAPLNPFTGG"
     gene            7868..9034
                     /locus_tag="PLJ64_05420"
     CDS             7868..9034
                     /locus_tag="PLJ64_05420"
                     /inference="COORDINATES: protein motif:HMM:NF037653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC92958.1"
                     /translation="MSPAPEAKSSAPARLASPVLAGRGLPGLARAAADVLGMPVAFLD
                     RSGIVLAVAGATQAQEAKLTAKEKGVATIELILGDTTVGEARYLGDGVDGDTLEVMSA
                     LAALEVERARSDEWGNEGAVSEFIRAMLDGSMTNTDDIVARAGEVGCDLSRGGGVLMI
                     RAHAGSAQEGDWRSRVLDMALRTIRAIASGAIAGMGESEEGAVVGILVPTNEEDRLVK
                     VEDALDRELPRSLSGLTVTISRSRLATGPDQIERAAREAQLALNVGEAEGRSPIAFED
                     TGAYRLLLGTSNEELMSFYSETVEPLVAYDEQYETDLVATVEAYLDSDANVPATAKQM
                     FTHRHTIRYRLERIRDLSGHDATATEGRERLGLGIKAMRVLGIASPRGRSKERT"
     gene            complement(9207..11546)
                     /locus_tag="PLJ64_05425"
     CDS             complement(9207..11546)
                     /locus_tag="PLJ64_05425"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HNC92959.1"
                     /translation="MRKFAVAFAALFMLVVVPNAFAAPPSSIFNGTIPCTTVGDGRTV
                     CQGPGGDTNATPGVNNAVANTVPSWDGTPIDVTVVLPDASTFGPGPYPLAMYFHGFGG
                     GKEGADGDLKRFTDLGFAAFSMTERGFKFSCGKPNAIIALNNDTPDACDKGFIHLMDT
                     RYEVRDAQYFAGLLADEDLIQPKKIGTAGASYGGAKSISLGALRNRVMLPDGSLIPWQ
                     SPEGKDMEIAVAAPIVPPSDFAYSLVPNGRTYDYTRFNTYLGPGGDAPFGVMKSAITN
                     GLASAGDSFSGEHGTDLDPLWDINGWKVMMNAGEPVDTAVATTMTDEMTTHHSAYYID
                     HSIEPSPMIIAQGLTDDLFPISESIRFYNRTKSEYPNSTVGLLFADIGHPRAPLEEPN
                     EQGRPADMELGYTTVEKWFDYYLNGTLPKPDDGVTAMSMVCPYSEPSGGPYTSDTWAD
                     FAPGEMRLSDPAKRVIAADGGDDNVASNFMTLIPACTQQQETEEPGIAQYDFPVSSGA
                     GYTLAGSATIIADVSVANGGESQIAGRLLEITPDGNQRLIARGVYRPESSGTQVIQLN
                     ANVYHFAPGTKMRIQLLPRDGSTGLPQLSSFRPSNDQQDVTIKNVDIRVPVREEPGSL
                     NGLVKSPSPKVLPDGYELATGFSSIGSISMADYIGNRDAKPGKLKVTAATVKGRQLTA
                     KVSCPAAAGSCAKAKLSFKGAAGRGKGVVLATRAGVTARPGKTATVKLNLTARARKLF
                     KDSKKGKRRIKGLKSLKATVLINGKSAGKITVKRTGKVN"
     gene            complement(11822..>12827)
                     /locus_tag="PLJ64_05430"
     CDS             complement(11822..>12827)
                     /locus_tag="PLJ64_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92960.1"
                     /translation="TGGDRAAGGTFNPAPSGTACATASGTPETGIANYETAAAPAGGF
                     TVMGSPTVIARYSYSSDEPPQVAARLVDISADGTTKTLVNRGVWRLPDTGFQVFQLNP
                     NGWHVDEGHKLRLELLPFDGGNADPVAGLLSNFGRPTNNEPAITVEDLELRVPVKEQP
                     GSLSGLVKAPAKRVLPNRPGVELAPGNEDIGSETIEQYRERNYPVVGKIKITGATVKG
                     KTLTAKVFCAGSNDSCARAKVSFKGAPKKGKGKGVVLATKSNVTGPAGKTTAVRFNLT
                     AKARALFKDTVKRKVTRKNGRKKVKKVKVSGLKSLRSQVLIGGKSAGFITVKRTGKVS
                     "
BASE COUNT         2047 a         4258 c         4283 g         2239 t
ORIGIN      
        1 ccgcctacgg tcgcatggaa gccgaggccg cctgatgccc ggccgcctca ccgtctgccc
       61 gaccccgatc ggcaacatgg atgacgtcag caagcgggtc cgcgaggccc tggtgatggc
      121 tgactatgtg gcctgcgagg acacgcgacg taccggccgg ctgctcgaac ggctcgagat
      181 ccgccccaaa ccgcgcctgg tttccaatca cgagggcaac gaggcctcac gggcggtgga
      241 actggtcaag cggatggaac ggggtgacaa gatcgccctc gtctccgacg ccggcatgcc
      301 cgcggtctcc gatcccggtt accggctgat ccgggcctgc atcgaccggg gcatcgaagt
      361 cgaggtgttg cccggccctt cggtgattcc ggtcgctctg gtcgcatccg gtctgccgac
      421 cgaccgctgg cggttcgagg gatttttgcc gaagcgggcg ggcgagatgg aacgggtgct
      481 gaattcggcc gagacggtga tcgccttcga atcaccgcga cgcatctcga actcgctccg
      541 ggccctctcc cagctcgccc cggaccgcca ggccgcggtc tgccgcgaga tgaccaagct
      601 ccacgaggaa gtctcccgcg gcaccctgac cgagctggcg atgcggttcc agggcgaggt
      661 caagggggag atcgtcctgg taatcggtcc ggcagcggcg gccgagcacg gccaggacat
      721 cgccttcgcc gtggaagcgc tgaagcgcct ggtcaagtcc ggggccaggc cgagggcggc
      781 cgcgaccgtg gtcgcctcgc tgaccgggac ccgggccaac gacctctaca aggccctgac
      841 cggccgggaa ccgcggcgtt gagcgccgtc gggtccaggg ttgcctacgc cacctgcgag
      901 gacaggattt cggattggga ggacgacctg ctctcggccg atctgctggc cgaacggggg
      961 gtggaggtcg aattcgcagt ctggaatgac cccgcggttg attgggaatc cttcgacctg
     1021 acggtaatcc gctcgacctg ggattacacg gagcggctgg aggaattcct tgcctggtgc
     1081 gagtcggtgg ggccggggaa gctccgcaac gtgccggcga tgatcctctg gaacaccgac
     1141 aagcgctacc tggccgaact ggacggggcc gggctgccgg tcccgccgac cgcgctggtc
     1201 gctcccggct cgaacccgcc gcccttcggc ggcaaggtgg tgatcaaacc ggtttccgga
     1261 gccggcgccc gggacacggg agtctttgac gaagcaaacg aggaaggtgg cctcgccctg
     1321 ctcgagaagc tcgcccgaca ggacgagatc gcaatgatcc agccctacat tccctggatc
     1381 gacgaacggg gagagacggc ggtgctgttc ttcggcggcc gcttcgccta cgcgctgaag
     1441 aaacgcgcct tcctgccgga atccggggtg gccccggtcc ggccggggac gaccgtggcg
     1501 gaagggatgt tcgccgagga tctgatgagc ctcgccgacg cgagcgaagc cgaggtcgaa
     1561 ctgggcgcga aggtcgtcgc ctggctggcc cggagattcg gctcggtacc gctctacgcc
     1621 cgcatcgaca tggtcagctc gccgcagggg gagcccgtcc tgatggaggt cgaggtgacc
     1681 gagccgagct tctacctggg actgaccacg ggactcgaga cgcccggacc ggaactgttc
     1741 gcggcggcgg tcgaagccgg cctcggctga gccacgcgga cgaaacaagt cctatgctgg
     1801 atctcccgtg agtttctacg tcaccacacc gatcttctac ggcaacggcg agccccatct
     1861 cggccacgcc tactcgacca tggccgccga catcctcgcc cggcacatgc gccagcgggg
     1921 tgaagacgtg tttttcctta ccgggaccga cgagcacggc gagccgatcg cgcaggcggc
     1981 cgagaagcag ggcctgactc cgcaggagct cgtcgaccgt agcgcccaga gcttccgcga
     2041 catggccgcg aaggtcgatg cgaccaatga cttcttcatc cgcaccaccg accagggcca
     2101 catcgagcgg gtccaggcgg tggttgacca ggtcaaggag aacggcttcg tctacgaggg
     2161 aacctacgag ggctggtact gcccccgctg cgccgacttc aagaacgaga acgagatcgg
     2221 gcccggggat acctgcctga tccacgaaat cccgctcgac cgggaaacgg agcagaactg
     2281 gttcttcagg ctctcggcct tccaggacga actcgagaag ctctacgaag agaacccggg
     2341 aatgatccag cccgacttcc gccgcaacga ggcgctcgcc ttcatccgcg gcggtctcaa
     2401 cgatgtctct ctttcccgct cccagatctc ctggggggtg ccgctgagct gggatccgga
     2461 ccaggtcatg tacgtctggt tcgacgccct gctcaactac tacacggccc tcggctacgc
     2521 ccgcgaggga gaagacctga ccggccgctt ctggcccgcc tcctggcaca tcatcgccaa
     2581 ggacatcctc aagttccaca ccgtttactg gccggccttc ctgatggctg ccgggatcga
     2641 ggtgccgcag cggatcttca tccacggctt cctgctgatg ggtgacaaga agatgtcgaa
     2701 gtcgctcggc aacgtgctcg acccgttcga ggtgatcgag aagttcgggg ccgacgccct
     2761 gcgcttctac tgcttccgcg aggtcagctt cggccaggac ggttcgatct cgaccgaggg
     2821 tttcgaaacc cgctacgagt ccgaactggc caacgacttc ggcaacctcg cctcacgcac
     2881 catcgcgatg atcagccgct accgcgacgg ggtcgtgccg gaggccgggc cggacccgct
     2941 gctcctggcg gatttcgaag gcatcaccga ccgcgtttcc gccctgctgg acgatgccca
     3001 gatcgggcag gccctggaag agatctgggt gctggtccgt cgcctcaacc gctacgtcga
     3061 ggaggcgcag ccctgggtcc tcgccaagga cgaggccgac gccgaacggc tcgaccaggt
     3121 tctctacagc ctggccgagg ggctgcgggt gctcaccctg ctgctccatc cctacctgcc
     3181 ggcctccacc gacatcctgc tcgaagccct cgccgagccc gaccagggcc tggctccgtt
     3241 tggcagccgg cccgggggcg tgaaggtcga aaagatcgat cccctgtttc cgaaactctg
     3301 aggaactagg atcggggcat gagaaaagcc aggagtctta cagcaggagt tgccatcgcc
     3361 gcaatcggtc tgggattgat tggcgcttca gtcgcaccgg cctctgcagc cgcgcgcccg
     3421 ctcagcgtca ggttcttcac tccggacggc aagcagcgcc tcgaggtgaa gcgcaaggtc
     3481 ggaatcggcg tcggctgcac caagaactgc gcggtggtgg tcgacatgaa gctggtcatg
     3541 cccaacacca agccgatcaa ggccagggtg aagggcggaa tcaaggcctt cagcgccaag
     3601 tcgccctaca tcgtgctgaa caatcccgcg ctcaggtacc tgaaactgac ctacccccgg
     3661 tcccggttct cgatcaaggt tactgcgacc gacatctcga acggcaggaa gacggtcaag
     3721 cgcagggtct acggcttccg cgcatagccg aacttgatcg attcgcattc gcatgtctcg
     3781 cgctgccccg agtcaccgga gaaggtgatc ggggcggccc gcgaggccgg ggtcgagagg
     3841 atcctcacgg tcggtctcga cgaggcttcc aaccgggagc agatcgccct ttgcgagcag
     3901 ttcgacgggc tgttcgcggc ggtcggaagg catccgaacg acgccgacgg cttcgatgac
     3961 gcggcggccg ccgacctcga gcagcccgcc gcccacccga aggtggtggc aatcggcgag
     4021 accgggctcg acttcttccg ggacacggcc gagcccgaca gccagcgact cgctttcatc
     4081 aagcaggccg acatcgccgc ccggctcgac ctgccgctgg tcatacattg ccgggatcag
     4141 caaggccggg atgacgcggt ctccgaaatc ttcgcgacgc tcgaggagtt cccggacctg
     4201 accgtgatcc tccactgttt ctccgccccg gactgggtcg agcgggcggc cgaacgcgac
     4261 tggtactgct cgtttgccgg caacgtcacc taccccgcca acgaggatct gcgcaccgcc
     4321 gcggccaggg tcccggccga ccggatcatg gtcgagaccg acgcgccgta cctgacgccg
     4381 cagtcccggc gccgggaccg gaaccagccg gccttcgtgg tcgagaccgc ggaactggtc
     4441 gccgggctgc ggggaatcga ctacgccgaa ctggacgaac tggtcaccgc caacgcagcc
     4501 cgcgtctacg gatggtgaag ctcggccaga acttcctggc cgacccgaac ctgctcgacg
     4561 cgatcgtccg ggatgccggc ctctcgcccg aagacgtggt cctggaagta ggtgcggggg
     4621 agggagtgct gaccgagcgc ctggccgaag tcgcttcgca ggttcacgtg atcgagctcg
     4681 accggggcct cgagccgggg ctgtccggcg tgatcggcag gccgaacgtg aacctggtct
     4741 gggccgacgc ggtgaagtac gacctggccg gactcgaccc ggccccgacc cggatggtcg
     4801 ccaacctgcc ctacgccgtg gcgaccccgg tgatcctccg cagcgccttc gagctgcctt
     4861 cgatcgagag ctggagcgtg atggtccaga aggagatcgg cgaccggctg cgggccgggc
     4921 cgggcagcaa gacctacgga gccccttcgg tcctgatcca gctgaccggc gaggtgcggc
     4981 aggttcgcaa ggtgagccgg accgtcttca agccccaacc ccgggttgac tcggcgatca
     5041 tccggatcga ccgcacctct cccggccccg acgaggcgac ccggcggctg gtccgggccg
     5101 gcttcgccca ccgccgcaag gcgatggccc gttcggtcga cctcgccatc ccgggcagcc
     5161 tcagggcggt ccgggacggg ctcgaagcgg ccgggatcga cccgggcatc cgggcagagg
     5221 cgctttcgcc cgcccagttc gcaaagcttt ccgggatgat cgatctcgct gccgatgact
     5281 gaacttgatc tccccctcga actgaacgcg ccggccaagc tgaacctctg cctttacctg
     5341 gggccgaggc gcaaggacgg cctgcatgaa ctcacctcga tcttcgagcc gctgaccctc
     5401 aacgacacga tccgggtaac cgaaggaggc ccggcggccc tggtcgacca ggtgatctgc
     5461 ccgggcgtgc cgggcgagaa cctggtcagt caggtgatca ggatgatgcg ctccgagggc
     5521 tgggatcccc ctctgctcaa ggtcgagatc gaaaagcgga tcccggtcca ggccgggctc
     5581 ggcggcggca gcgccgatgc cgcggcaatg ctccgcctcg gggttggaga ggtcgaggac
     5641 ctggccgggc tcgccctgct gctcggcgcc gatgtgcctt cgcagctcaa tccgaagccc
     5701 ctgctggtcg agggggtcgg gcagaaactg accaccctcc cggcgccggc cgaacactgg
     5761 gtcgttctgc tgcctttcga gcgaggactt tcgacccggg cggtgttcga tgaagccgac
     5821 cggatgaagc tcggccggag ccaggaagag atcgacgaca tttccgggcg cctctgggcg
     5881 gttgccacca acgcggtgtc cccgctcgcc tacccggccc tgctgatcaa cgacctcgag
     5941 ccggccgcga tttccctcaa gccggaaatc gggctcggca aggagcggct ggaggcggcc
     6001 ggggcgggat tcaccgggat gaccggcacc ggccccaccg tcttcgggct tttccccgac
     6061 cgggaatcgg cagaggcggc ggccgccgcc gttggtccgg aggcgctcgt ctgccagggc
     6121 ggcgtggtct gatttcgcgc tcatgatcaa ggtcccggag acgaaaaagg gcaaacggct
     6181 gaccctcgcc ggcatcgcgg cggcggccct cgtcatcttc atcctctacc gggagttctt
     6241 tcccgagctc gacctccagc agctgctgga cgacttcgcg aacttcctcg gtgcctggac
     6301 ctacctcgtc gtcggcctgc tcgccttcct tgaaaccggc gccttcgtcg gcctgatcgt
     6361 ccccggagaa acggcgatgc tgatcggcgg tgcggtcgcc ggtcaggggg tgatcaacgt
     6421 cttcctgctg atcgcgattg cctggctgat ggccttcctc ggtgattcga ccagcttctg
     6481 gctcggccac cggcttggcc gcgaattcat cctcaaacac ggcccgaagt tcgggatctc
     6541 ggcggaacgc tacgaacagg tcgaggacta cttcgaaaag catggcggca agaccgtcct
     6601 gatgggccgg ttcatcgggc tggtccgggc cctttccccg tttgtggcgg gcagctccgg
     6661 catgcggtac cgggccttcg cgccctacag catcctcggc tccggcctcc agatcaccct
     6721 gcacatcctg gccggctatt tcttcgcccg cagcatcgaa gcggcggccg agtacgtcgg
     6781 gctggcggcg ctcgtcatcg gcacgatgat cgtggtctcg gtcgcctcct atctcgcctg
     6841 gaaatacctg cgcgaaccga ataaccgggc cagggcggtc gagtggatgg aaggccgctg
     6901 gtacagcgcc tggctggtgc gactggcccg tcgctacgag acgcagttgc gctggatcca
     6961 ggaccgcttc acgccgggcg ggactttcgg tctcgaggtg acgacgctct tcgcgatcat
     7021 cgccgtggcc tcattcgtgc tcttcgccta cgccgggatt ctgctcgacg acggtggccc
     7081 gacccccggc gacctgcggg ccttcgattt cagggacctg atcgagaccg gctggctgac
     7141 ctccatcgcc aaggtagtga cggtgctcgg ctcccaggtg gtgctgctgc ccctgatcac
     7201 cgtgaccgcg gtgatactgg cggtccggca gcactggtcg gaagtgctgg tgctggtcct
     7261 ttcctcgatt gcgatcgcgt tcggaatcga ctggatcaag gactgggtcg cgcggccccg
     7321 ccccgatggc gggatgatcg aggtcgccgg ctacgcctac ccctccgggc acgccgccca
     7381 ttcgatcttc tatgtctggc tctcggtcac catcgccgtc cggctccgcc ccgacatggc
     7441 ccgcaaggct gccctgatca ccgggggcat tctgctggcc gcgctggtcg gcctgagccg
     7501 ggtctatctc ggtgtccact acctgagcga cgtcttcgga ggctgggctc tcggggcact
     7561 ctgcttctcg tttttcgcgg tctgggccct gcttctgggc cagttgcgca aaaattaggc
     7621 cgatggtgct cgccgtttca acccagatct ggtatttcgg cgccgccggc ctcgtgagtc
     7681 tcgccgcatt cgtcggcctg atcctggtcc cggcggtgag ttcgtacggg cgctggcacg
     7741 agcgtttcgc ggccggtttc ctctccctgc tgatcttcgg ggggctgatc acgatcggcg
     7801 tcatcgcggg actggcgtgg gtactgatct cgaacggcat cgcgccgctc aaccccttca
     7861 cgggcgggtg agccccgccc cggaagccaa gtcctcggcg cccgcgcgcc tggccagtcc
     7921 ggtccttgcg ggtcgtggat tgccgggcct ggcccgggcc gcggccgacg tgctgggaat
     7981 gccggtggcc tttctcgacc gttccgggat cgtgctggcc gtggcgggag ccacccaggc
     8041 gcaggaagcg aagctgacgg ccaaggagaa gggcgtcgcc acgatcgaat tgatcctcgg
     8101 tgacaccacg gtcggtgaag cccgttacct cggagacggc gttgacggcg acaccctcga
     8161 agtgatgtcg gccctggccg ccctggaagt cgagagggcc cgttccgacg aatggggcaa
     8221 cgagggcgcc gtgtcggaat tcatccgcgc gatgctggac ggctcgatga ccaacaccga
     8281 tgacatcgtc gcccgggccg gggaggtcgg atgcgacctt tcccgcggcg gcggagtcct
     8341 gatgatccgg gctcacgccg gctcggccca ggaaggtgac tggcgctccc gggtgcttga
     8401 catggccctg cgcacgatcc gggcgatcgc ctccggcgcg atcgccggca tgggtgaatc
     8461 cgaggaaggg gccgtggtcg gcatcctggt cccgaccaac gaggaagacc gcctggtcaa
     8521 ggtcgaggac gcgctcgacc gggaacttcc gcgctcgctc tcgggcctga ccgtaaccat
     8581 ttcccgctcg cgcctggcca ccgggccgga tcagatcgag cgggccgccc gggaagccca
     8641 gctcgcgctc aacgtgggcg aggccgaggg ccgctccccg atcgccttcg aggacacggg
     8701 cgcctaccgc ctgctgctcg gcaccagcaa cgaggaactg atgagcttct attcggagac
     8761 ggtcgagccg ctggtcgcct acgacgagca gtatgaaacc gacctggtcg ccacggtcga
     8821 ggcctatctg gacagcgatg cgaacgttcc cgccaccgcc aagcagatgt tcacccaccg
     8881 gcacacgatc cgctaccgcc tcgaacgcat ccgcgacctc tccggccacg acgccaccgc
     8941 caccgaaggc cgggaacgcc tcggcctcgg aatcaaggcg atgcgcgtcc tcggcatcgc
     9001 ctcaccccgg ggccgctcca aagaacgaac ctagcccggg caacgcaccc aatccggtct
     9061 cgaccggttc cggccctgat tcgggcagct gccgcaggtt tgcaaccgga gaggtcgcaa
     9121 acctgcggca ccgaccaggg tcgaggccgt gaacccggcc tggaggcagc tcggacttcg
     9181 ggaagactcc tccgggccgg ttgcggttag ttgactttgc cggtcctctt gacggtgatc
     9241 ttgccggcgg acttgccgtt gatcaggacg gtggccttga gtgacttgag gcccttgatc
     9301 ctgcgcttgc ccttcttgct gtccttgaac agcttgcgcg ccctggcggt caggttgagc
     9361 ttgaccgttg cggtcttgcc gggcctggcg gtcacccccg ccctggtggc gagcactacg
     9421 cccttgcccc tgccggcggc gcccttgaag gaaagctttg ccttcgcgca ggagccggcc
     9481 gcggccgggc aactgacttt ggcggtgagc tgcctgccct tgaccgtggc agcggtcacc
     9541 ttcagcttgc ccggcttggc gtcgcggttg ccgatgtagt cagccatgct gatagatccg
     9601 atcgagctga acccggtcgc cagctcgtag ccgtcgggca ggaccttcgg ggacggactc
     9661 ttgaccaggc cgttcagcga gccgggctct tcccgcaccg gaaccctgat gtcgacgttc
     9721 ttgatcgtca cgtcctgctg atcgttcgac gggcggaaag aggagagctg cggcaacccg
     9781 gtgctgccgt cccgaggcag cagctggatc cgcatcttcg tgcccggagc gaagtggtac
     9841 acgttggcgt tcagctggat gacctgggtg cccgagctct ccggtcggta gacgccgcgg
     9901 gcgattagtc gctggttgcc gtccggggtg atctcaagca gccggccggc gatctgcgac
     9961 tcgccaccgt tggccaccga aacgtcggcg attatggtcg cggaaccggc cagcgtgtat
    10021 cccgccccgc tcgaaacagg gaagtcgtac tgggcgattc ccggttcctc ggtttcctgc
    10081 tgctgggtgc aggccgggat cagggtcatg aagttgctgg ccacgttgtc gtcgccaccg
    10141 tcggcggcga tcaccctctt ggccgggtcg ctgagccgca tctcaccggg ggcgaagtcg
    10201 gcccaggtat cggacgtgta aggcccgccg gaaggctcgc tgtacgggca gaccatgctc
    10261 atcgcggtaa ccccgtcatc gggtttcggg agggtgccgt tgaggtagta gtcgaaccac
    10321 ttctccacgg tcgtgtaacc cagctccatg tcagccgggc gtccctgctc gttgggctcc
    10381 tccagcgggg cccggggatg gccgatgtcg gcgaacagca gaccgacagt ggagttcggg
    10441 tactcgctct tggtccggtt gtagaaacgg atcgactcgc tgatcgggaa gagatcgtcg
    10501 gtcagcccct gggcgatgat catcggtgac ggctcgatgc tgtgatcgat gtagtaggcg
    10561 gagtgatggg tggtcatctc atccgtcatc gtcgtggcga ccgccgtatc gaccggctcg
    10621 cccgcgttca tcatgacctt ccagccgttg atgtcccaga gcggatcgag gtcggtgccg
    10681 tgctcgccgc tgaaggagtc gccggccgag gcgaggccgt tggtgatcgc ggacttcatg
    10741 actccgaacg gtgcgtcgcc gccagggccg aggtaggtgt tgaagcgggt gtagtcgtag
    10801 gtccggccgt tcggcaccag ggagtaggcg aagtcgctcg ggggcacgat cggggcggcg
    10861 accgcgatct ccatgtcctt gccctccggg ctctgccacg ggatgagaga accatcgggg
    10921 agcatcaccc ggttcctcag ggcgcccagc gaaatcgact tggcgccgcc gtaggaggct
    10981 ccggccgtgc cgatcttctt cggctggatc aaatcctcgt cggcgagcag gccggcgaag
    11041 tactgcgcat ccctgacctc gtagcgggtg tccatcagat ggatgaagcc cttgtcacag
    11101 gcgtcagggg tgtcgttgtt cagggcgatg atcgcgttcg gcttgccgca ggagaacttg
    11161 aagccgcgct cggtcatcga gaaggcggcg aagcccagat cggtgaaccg cttgaggtcg
    11221 ccgtcggcac cctccttgcc gccgccgaaa ccgtggaagt acatggccag cgggtaggga
    11281 ccggggccga aggtgctggc gtcgggcagg acgacggtga cgtcaatcgg ggtgccgtcc
    11341 cagctgggga cggtgttggc gaccgcgttg ttcacaccgg gcgtggcgtt ggtgtcaccg
    11401 cccggaccct ggcagacggt tcgaccgtct ccaacggtcg tgcaggggat cgttccattg
    11461 aagatcgagc tcggcggcgc ggcgaaggca ttgggcacga cgacgagcat gaaaagtgca
    11521 gcgaacgcta cggcgaactt acgcatttga tccctgtttc tccctattga aacggttgcc
    11581 gctccctgcg gcttcccttg gacaactctc tcgggcacgc gccggttggc acatcccctg
    11641 gctattgaga ttaccacccg ttcacgaaac caacacgaaa aggagcccgc cgtcgacgga
    11701 ctccctgagc atctcgttgg gtcaggacgg taccgggggt acctgaaaag tgactcctta
    11761 atcagagcgc gaagcgctcg ttacttttca ggtacccccg gtgtcgtccg gagactctcg
    11821 tttagctgac cttgccggtc cgcttgacgg tgatgaagcc ggccgacttg ccgccgatca
    11881 gcacctggct gcggagcgac ttgagtccgc tgaccttgac cttcttgacc ttcttgcggc
    11941 cgttcttgcg ggtcaccttg cgcttcacgg tgtccttgaa cagcgcgcgg gcctttgcgg
    12001 tcaggttgaa cctgacagcc gtggtcttgc cggccgggcc ggtgacgttg ctcttggtcg
    12061 ccagtacgac gcccttgccc ttgcccttct tcggggcacc cttgaaggac accttggccc
    12121 gggcgcagga gtcgttcgag ccggcgcaga acaccttcgc ggtcagggtc ttgcccttga
    12181 cggtggcgcc ggtgatcttg atcttgccga ccaccgggta gttccgctcg cggtactgct
    12241 cgatggtctc cgagccgatg tcctcgttgc cgggggcgag ttcaacaccc ggacgattcg
    12301 gcaggacccg cttggcgggt gccttgacca gaccgctcag cgacccgggc tgttccttga
    12361 ccgggaccct gagttcgaga tcctcgacgg tgatcgccgg ctcgttgttg gtcggacgcc
    12421 cgaagttgga cagcagtccg gcgaccggat cggcgttgcc gccgtcgaag ggcagcaact
    12481 cgagacggag cttgtggccc tcgtcaacat gccagccatt cgggttcagc tggaagacct
    12541 ggaaaccggt gtcgggcagg cgccagactc cgcggttgac cagtgtcttg gtggtgccgt
    12601 cggccgagat gtcgaccagg cgcgccgcaa cctgcggcgg ctcgtcactg ctgtagctgt
    12661 acctggcgat cacggtcggc gagcccatca ccgtgaaacc accggccgga gcggcggcgg
    12721 tctcatagtt ggcgatgccg gtctccgggg ttcccgacgc ggtggcacag gccgtgccgg
    12781 aaggtgccgg gttgaacgtg ccgccggcag cccggtcgcc accggtc
//
LOCUS       DAOQKN010000057        12734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_2304_length_12734_cov_5.300024, whole genome shotgun sequence.
ACCESSION   DAOQKN010000057 DAOQKN010000000
VERSION     DAOQKN010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2304_length_12734_cov_5.300024"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..1196
                     /locus_tag="PLJ64_05435"
     CDS             81..1196
                     /locus_tag="PLJ64_05435"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNC92961.1"
                     /translation="MSVLAILAGAAFATLAMIGPAAAAGKSLDYVALGDSYSAASGVL
                     PLDPEAPVQCLRSTLNYPHVLAAKLGAELTDVTCGAAETRHFGESQYPGVAPQLDALS
                     KDTDLVTMTIGGNDSSVFIGSILSCGTLGVASLGQGSPCKDTFGDQFEDTIRNTTYPS
                     LVEALNAVHAKAPKARVAILGYPWIMPETDGCYPQMPVSKGDVPYVRSLQRTLNDAVG
                     RAARETGSVFVNLNGVSNGHDACQAPGTRWVEPALLGENPVIVHPNALGEAKMAEQAF
                     KVLSKSGTPAPAAPRTRISKVLISNGKRRATFRLESTGSAPTFRCRLDGQGFRRCRSP
                     KTYRGLKTGSHTFRVVAIDGNGLADRTPAVFRFRIRG"
     gene            complement(1180..2931)
                     /locus_tag="PLJ64_05440"
     CDS             complement(1180..2931)
                     /locus_tag="PLJ64_05440"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92962.1"
                     /translation="MPNPRNFIPVLAALATLLLAPASFAAKDPAFVPSRNSPIQTAGT
                     PIGVAPYDQASSESGLLVLNRRPAGIQVFENRQETPLLPVHRFRVKEDPLSLQTFTDT
                     WSEVDAVGVVRHDGSFSFYRVTHSSSKGYGLVSGNIGVDIGPVTALAKYHGLGIDSAP
                     FGRVKVVAVDTADDSLILVSQVPGFPDAIEATVPVGDQPVDIYSENAIAMFVVNRGSD
                     DISVVRYFQDPDETGEIGQGNYGHWEVTDTAPVGESPVAIAAIPSRQGRNLFAVANSG
                     SDDVSILRLNPDSSMDELGRVPVGDRPVALTSVQMTANGRWRLAVANAGSDDVTIIEG
                     EFNGTFAATDTIPVGSNPVSITSGDFDEYFDADLAVADRASGQVSVLLDNEPSGTCRG
                     GAAKLLRGGQYSDSLFGTHNPDQILGLTGDDEIHGGKDGFDCLFGGKGKDSLDGYKLG
                     DLLDGGPDADTLTGGTGTDLVIGGAGDDFLCENLEEEMKQDACGVPYRDYSSWGRIGR
                     PERDRLFGGPGNDRIQADYGRDLVSGGSGRDDIFSRDGARDLVTCGAGRDRALVDDRD
                     RVRGCETVKRIEVSRES"
     gene            complement(3008..3607)
                     /locus_tag="PLJ64_05445"
     CDS             complement(3008..3607)
                     /locus_tag="PLJ64_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92963.1"
                     /translation="MGARIIENGEIGISWVMEDEAMARASHALTDGEKVWLVDPVDDD
                     AAIERALTLGKPAVVFQLLDRHNRDCAQVADRLGIDHVALPLELRGTPFEAIEVVENR
                     IWKERALWWKKTKTLVVAEAVGASRMFNPSEAGAGVHIGLRATPPRKQLGTYNPRHLL
                     MGHGNPINGKQATVALQQALDRSRRDLPGTMLKIPFAFR"
     gene            3669..5372
                     /locus_tag="PLJ64_05450"
     CDS             3669..5372
                     /locus_tag="PLJ64_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92964.1"
                     /translation="MRGVKQSVNSGGVLWLAALVTCFALLPAAKSEAIPIVKNVQLAG
                     SGGQWKLLVPMTLPLEVSGFEVTGKAGVELPGGQWTKTWQTRVHSGSLREADQRDFFL
                     HVHGIPVSRKLARRILRAGKEASAKVTVRFTGPSADPEIRPDGSLGLSQKGIPRARPG
                     VCETLPRVLLEGSKIRSRTLRFPVCGKDLSWELVQRPLKGDYLSRADVFRYSRRSGQV
                     GADELELAGSFRGRQVTTQRIQFRLGNVPASSVSVAAFGDSVTAGFGYFGATGKPMTL
                     GQLLDCKPGATTLNDACSSNSYNRNSSVGSKPNYLQDFGLSRNISWAAQWANEYGITE
                     YENYAVTGSAPADWLPNGQFHQTLQNIESQNPDYILMTLGANPLLDDVLFDVDTMGCA
                     LESDLFGDFRQCVLDAFASVNLDSNMNQIFTDLVQNSSSHVVLMGYPFTIPSSALAYS
                     AAQLEMMESLLNEVIADEAESVSSSRITVVTPPRFNVGIDMEPLYPSNYSCSLLGYKV
                     DGPSVQSTPTQDELEIDHPLSFCSGPSFGKPWVISGDTGIHPSAAGYLQMSSQIPAPG
                     S"
     gene            5414..6076
                     /gene="msrA"
                     /locus_tag="PLJ64_05455"
     CDS             5414..6076
                     /gene="msrA"
                     /locus_tag="PLJ64_05455"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNC92965.1"
                     /translation="MFFGRDKTTMPAPEDSLPGRSGYAFPVPDNHTVLGTPIQGPFPE
                     GIEVAHFALGCFWGAERLFWNLDGVYTTAVGYQNGFTPFPTYEEVCSGKTGHAEAVLV
                     AFDPEKVSYERLLEVFFSEHDPTQGMRQGNDVGTQYRSAVFTVGDRQKEIAEMARDTY
                     QRALNERGLGEITTEIEEAGPFYYAEGPHQQYLDKVPNGYCGLAGTGVACQVPVAGGS
                     ST"
     gene            6159..8432
                     /locus_tag="PLJ64_05460"
     CDS             6159..8432
                     /locus_tag="PLJ64_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92966.1"
                     /translation="MTPSLASYIAGTLQIAVVVGAVWYGSWWLRRWIVPEFSGALARL
                     AELTIAFALIILSLELIGSVGLLKEVWITVACVVIPLIGAGLAWKFAPRDVEEIAAPA
                     VPTWAFLVAVAVASWCVAEWAFPTQLSLDQGMFGGDTTWYHMPAAARFIQDSSIIPLH
                     FTDALRLAVWFYPQNSELMHGALMGVMGSDWLSPVFNMLPLGIALLACWCVGRPYGVG
                     PATLVAGAMLLSAGVMIETQPGEGRNDIVCFAFLIAFVAFLINGHQRRAPSSGAVDEK
                     PDPNAPLLDKGPLILAGIAAGIAISIKVSMLAPVGVIFLGMILISGKGRRVTTALCLG
                     ISMFIVGGYWYVRAMLYTGGNPVPAVGWGPLHLPQPDQMPLDPRPRFSVAHYITDPSI
                     YRWWFFPRLDDAFGVLFPLILVMLAAAALWLVFKSRNKIVRVISAAALITAFVYVFTP
                     LTAAGQEFQPRGFFTNTRYLLPGLLLAAVMLPLIRQLREPQARAKKTLIFLTVVYAIT
                     VLTSPKWYPSFLPGAIFLTLAIVWAPVGLSWMKDNTRVPKYVIVGCVAAIAIIGAIWG
                     RGEEVGYADKHYTRTTLFLQEGGPQDAFEFTRNLKDKRIALAGSGEIFFGQYGFYGVD
                     RSNHVQYIGQEGDNGTYRLITNCREFINTINEGDYDYLITSEYTQDAPEADYWFPVRA
                     WVDGDPNAELVVSEDDITPQPDYVYKINGKLDPDRCAKLDQAAIRQVRTDALQAIKDR
                     EAEAAAAEAAAESSDSQ"
     gene            8481..8554
                     /locus_tag="PLJ64_05465"
     tRNA            8481..8554
                     /locus_tag="PLJ64_05465"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8515..8517,aa:Arg,seq:tct)
     gene            8552..9031
                     /locus_tag="PLJ64_05470"
     CDS             8552..9031
                     /locus_tag="PLJ64_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92967.1"
                     /translation="MPSSRCEEGDDRDNQDQAAVDEGGGVEAVPVRGEAADQGGDAEG
                     DVADHVGEGEELATFVGMGEGGKYAEGTERRGSEADSGNQGPGKGERGQVEQQADQID
                     RDSGGQPACREADHGHRLAVTEPIDNHGSGPAECRDHDRAGQVGVILDEARCDRRTQ"
     gene            9066..9518
                     /locus_tag="PLJ64_05475"
     CDS             9066..9518
                     /locus_tag="PLJ64_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92968.1"
                     /translation="MIGSSEKNRGLRWAGRPTFGNSDSRAPGFMVEVSGERQTATAAN
                     PSAASRIRKIRVVGQGANWISTPQPSAPIARPASGATPFTTPLRFGSSSGCRSTITAL
                     SVASATPVARPWITLARASIATPPESRNSRRERASKAIAPSSTGRLPR"
     gene            9945..10394
                     /locus_tag="PLJ64_05480"
     CDS             9945..10394
                     /locus_tag="PLJ64_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92969.1"
                     /translation="MSSIEDAIQQLETSASKLKQLSVEESRAIREAVRVATREATAKV
                     KAEYKDKKDKARKEARDAEKSIKDAQSRIQKALGSEKGGSTGGKRAKRGEREAQFVAY
                     VKENPDSKLADIARGIGVQNSAANGLAKKAVAAGKVKKSAQKTYKAA"
     gene            10514..11899
                     /locus_tag="PLJ64_05485"
     CDS             10514..11899
                     /locus_tag="PLJ64_05485"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNC92970.1"
                     /translation="MNVVRRGSLAFLATVAALGIFMSGSASAKLNVMTVQGTPAPGTP
                     AKYNKFKVVKQGPAKAKKILLLEPGTSAGATNFRPLGNALVGQLKGWQVWSIERRENL
                     LEDHSMLEKYLKGDATNEEMLDYYLGWILDGSVQPHFEGKTTAETQYAKKWGLSIVVH
                     DLRKVVNMARKGGRKVVLGGHSLGGSIVTAYATWNFNGTPGAKGLSGLVFIDGAGARE
                     AADLPTAGEAQANLDSLNAESASPFITLVPPLPWAAGVFNAMGSTAALQDPDSRSLME
                     DFPLLPDSLKPPVSATNLAQYAYAIDADTGPENLQLVQSHVGDLADSGDPRGWVNGQL
                     GTAQRAAQVFTEIDGMDGTSWYHPLRLSIDAGAINNGIATPAQQVYGIKTTMGKRVHL
                     PMYSFDTSLGDGRVKRATDALAAQSKVPKSWVRTVVRKETYAHIDPLSATPSKNDFIK
                     TLVPFLKKQVK"
     gene            complement(11963..>12734)
                     /locus_tag="PLJ64_05490"
     CDS             complement(11963..>12734)
                     /locus_tag="PLJ64_05490"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNC92971.1"
                     /translation="VEGTLLGGHIRLFEESGTEVHHHLGVSALSDHAVVSARSAVKVP
                     DDLPFDIAALFGCAVLTGAGAVLNTGAAALPARSVAVFGLGGVGLAALLAAKSQNPET
                     LVAVDTVPAKLELARELGADLTIQAGPDLVDSIREATGGGVDLAIETVGNENVLADAY
                     AATGRGGTTVTAGLPHPSRKLEIPAISLVAEERTLKGSYLGSCVPRRDIPAFIRMYRD
                     GELPVEKLLSHRLTPDQINEGFDRLARGEAVRQIVIFD"
BASE COUNT         2285 a         4163 c         4164 g         2122 t
ORIGIN      
        1 ccggggtagt cacagagcgc tcaggaacgc tggtgttctc gactaccccg ggagatttga
       61 tgaagcttcg cagtcgttgt gtgtccgtcc tcgcaattct ggccggtgcg gcattcgcca
      121 cgctcgccat gatcgggccg gcggccgcgg ccggcaagtc actcgactat gtggcactcg
      181 gcgactccta cagcgccgct tccggggttc tgccgctcga ccccgaagcc ccggtccagt
      241 gcctgcgctc gactcttaac tatccgcatg ttctggctgc aaagctgggc gccgagctga
      301 ccgacgtgac ctgcggcgcg gccgaaacca ggcacttcgg cgagtcccag taccccgggg
      361 tggcgccaca gctcgacgcg ctctcaaagg acaccgacct cgtcacgatg accatcggcg
      421 gcaatgacag cagcgtcttc atcggctcga tcctgagctg tggcacgctc ggggttgcct
      481 cgctgggtca gggcagcccc tgcaaggaca ccttcggcga ccagttcgag gacacgatcc
      541 ggaacaccac ctacccgtca ctggtcgaag ccctgaacgc tgttcacgcg aaggcgccga
      601 aggcccgggt cgcaatcctc ggctacccct ggatcatgcc cgagaccgat ggctgctacc
      661 cgcagatgcc ggtctcgaag ggtgacgttc cctatgtccg ctcgctgcag cggacgctga
      721 acgatgcggt tggacgcgcg gcccgtgaaa ccggttcggt cttcgtcaac ctgaacggcg
      781 tgtcaaacgg gcacgacgcc tgccaggccc cgggaacccg ctgggtcgag ccggcgctgc
      841 ttggcgagaa cccggtgatc gtccacccga acgccctcgg tgaagcgaag atggccgagc
      901 aggccttcaa ggtgctttcg aagtcgggca cgccggcgcc ggccgctccc cgcacgcgga
      961 tcagcaaggt gctgatcagc aacggcaagc ggcgggccac cttccggctt gagtcgaccg
     1021 gctccgctcc gaccttccgg tgccgccttg acggtcaggg tttccggaga tgccggtcac
     1081 cgaagaccta ccgtggcctg aagaccggca gccacacttt ccgggtcgtg gcgatcgacg
     1141 gcaacggcct tgcggacagg accccggccg tcttcaggtt caggattcgc ggctgacttc
     1201 gatccgtttg accgtttcgc aaccgcgcac gcggtcccgg tcgtcaacca gcgcccgatc
     1261 ccggcccgca ccgcaggtga cgagatcccg cgcgccgtcg cggctgaaga tgtcgtcccg
     1321 gccggagccc ccgctgacca ggtcgcgtcc gtagtcagcc tggatgcggt cattgcccgg
     1381 gcccccgaac agccggtccc gctcgggtcg gccgatgcgg ccccagctcg agtagtcgcg
     1441 gtaggggacg ccacaggcgt cctgtttcat ctcctcttcc aggttctcgc agaggaaatc
     1501 gtcgccggcc ccgccgatca caaggtcggt cccggttccg ccggtcaagg tgtcggcgtc
     1561 cgggccgccg tcgaggaggt cgccgagctt gtagccgtcc aggctgtcct tgcccttgcc
     1621 gccgaagagg cagtcgaagc cgtccttgcc tccgtgaatc tcgtcatctc cggtcagccc
     1681 gaggatctgg tccgggttgt gggtgccgaa cagcgaatcc gagtactggc cgccgcgaag
     1741 cagcttcgcg gcaccgccgc ggcaggtgcc ggagggctcg ttgtcaagca ggaccgagac
     1801 ctgaccactg gcccggtcgg ccacggcgag gtcggcgtcg aagtattcat cgaagtcacc
     1861 cgaggtgatc gagaccggat tggagccgac tgggatcgtg tccgtggcgg caaaggttcc
     1921 gttgaattca ccctcgatga tggtcacgtc atccgagccg gcgttggcga cggccaggcg
     1981 ccagcgaccg ttcgccgtca tctggacgga ggtgagggcg accggccggt cgccgaccgg
     2041 gaccctgccc agctcatcca tgctgctgtc agggttcagg cgcaggatcg agacatcatc
     2101 ggatccggaa ttggcgacgg cgaagaggtt ccggccctgc cgggaaggga tcgccgcgat
     2161 cgcgaccggg ctctctccga ccggggccgt gtcggtgact tcccaatggc cgtagttgcc
     2221 ctgcccgatc tccccggtct catcgggatc ctggaagtac cggacgacgc tgatgtcgtc
     2281 agagccccgg ttcaccacga acatggcgat cgcattctcg gagtagatgt cgaccggctg
     2341 atcgccaacc ggcaccgtcg cttcgatcgc gtcggggaag ccgggaacct ggctgaccag
     2401 aatcagggag tcatcggcgg tgtcgacggc cactaccttc acccggccga aaggggccga
     2461 gtcgatgcca agcccgtggt acttcgccag ggccgtgacg ggaccgatgt cgacgccgat
     2521 attgccgctg accaggccgt aacccttgct gctggagtgg gtcacgcgat agaacgagaa
     2581 gctgccgtca tggcgcacta cgccgaccgc gtcaacctcg ctccaggtat cggtgaaggt
     2641 ctggagggaa agaggatcct ccttgaccct gaacctgtgg accggcagca gcggcgtttc
     2701 ctgccggttc tcgaagacct gaatgccggc cggccggcga ttcaggacca gcagccccga
     2761 ctcgctgctg gcctggtcat acggagccac cccgatgggc gttcccgccg tctggatcgg
     2821 cgagttgcgg gaaggcacga aggcgggatc tttggccgcg aaggaggccg gggcgagcag
     2881 cagggtcgcg agggcggcga ggaccgggat gaagttgcgg gggtttggca tctgcttcct
     2941 tacagactgg acgaatcgac tcaccgaaag aaaccccgcg caatggggga gtttcgctct
     3001 ggagccctca gcggaaggcg aaggggatct tgagcatggt gccggggagg tcccggcggg
     3061 agcggtcgag cgcctgctgg agggcgactg tggcctgctt gccgttgatc gggttgccgt
     3121 gccccatcaa caaatgcctc gggttgtagg tgccgagctg cttgcggggc ggggtggcgc
     3181 gcaggccgat gtgaacgccg gcgccggcct cgctcggatt gaacatcctg ctggccccga
     3241 ccgcttcggc cacgaccaag gtcttggtct tcttccacca gagcgcccgt tccttccaga
     3301 tccggttctc gaccacctcg atcgcctcga aaggagtgcc ccggagttcc agcggcaggg
     3361 cgacatggtc gatgcccagc cggtcagcta cctgcgcaca gtcgcggttg tgccggtcaa
     3421 gcaactggaa gaccacggcc ggcttgccga gggtcagggc ccgctcgatc gcggcgtcgt
     3481 catcgaccgg atcaacaagc cagaccttct cgccatcggt gagggcatgg gaagcccggg
     3541 ccatcgcctc gtcctccatg acccagctga tcccgatctc gccgttctcg atgatcctcg
     3601 cgcccacggg cgggagtcta gacccgcagc cgctccgatc ggccgcaccg gggtttaagg
     3661 tatgcgaaat gcggggagtg aagcagagcg tcaattcggg cggggtgctg tggctggcgg
     3721 cgctggtcac ctgcttcgcc ctcctgcccg ccgccaagtc ggaagcgata ccgatcgtga
     3781 aaaacgtcca gctggccgga agcgggggac agtggaaact gctcgtcccc atgaccctcc
     3841 cgctcgaagt ctcggggttc gaagtgacgg ggaaggcagg ggtcgaactc ccgggcgggc
     3901 agtggacgaa gacctggcag acgcgagtcc attcgggatc gctccgggaa gccgaccagc
     3961 gcgacttctt cctccacgtc cacggaattc ccgtttcgag gaagctcgcg cgccggatcc
     4021 tccgggccgg caaggaagct tccgcgaaag tcacggtcag gttcaccggg ccctccgctg
     4081 atccggaaat ccgacccgac gggtctcttg gcctgtcgca gaaaggcatc ccgcgggcgc
     4141 ggcccggggt ctgcgaaaca ctgcccaggg ttctcctcga aggcagcaag atcagatcac
     4201 ggacgctccg tttccccgtt tgcggaaagg atctctcctg ggaactcgtc cagcgacctc
     4261 tcaagggcga ctacctgagc agggcggacg tgttccgcta ctcgcggagg tccggtcagg
     4321 tcggtgccga cgaactggaa ctcgccggct ctttccgggg gcgccaggtg acgacccagc
     4381 ggattcagtt ccgtctcggc aacgtgccgg catccagtgt ctcggtcgcg gcgttcggcg
     4441 actcggtgac cgccggcttc gggtatttcg gggcgaccgg caagccgatg accctcgggc
     4501 aactgctcga ctgcaaaccg ggagctacga ccctgaacga cgcctgctcc tcgaactcct
     4561 acaaccgcaa cagctcggtc gggagcaagc cgaactatct gcaggacttc gggctctcgc
     4621 ggaacatttc ctgggccgcg caatgggcaa acgaatacgg catcaccgaa tacgagaatt
     4681 acgcggtgac cggttccgcg ccggccgact ggctgccgaa cggtcagttc catcagaccc
     4741 tccagaacat cgagtcgcag aacccggact acatcctgat gacgctcggg gccaatccgc
     4801 tgctcgatga cgtgctcttt gacgttgaca ccatgggctg cgctctggag tcggatctct
     4861 tcggcgactt ccgccaatgc gtcctcgacg ccttcgcctc ggtcaacctc gactcgaaca
     4921 tgaaccagat cttcacggac ctggtccaga actcgagttc gcatgtggtg ctgatgggtt
     4981 acccgttcac gattccatcc tcggcactcg cctacagcgc ggcccagctc gagatgatgg
     5041 aatcgctgct caacgaggtg atcgcggacg aggccgaatc ggtctcgagc agccggatca
     5101 ccgtggtgac cccgccccgc ttcaacgtcg gaatcgacat ggagccgctc tacccctcga
     5161 actattcctg ttcgctgctc ggctacaagg tcgatggccc gagcgttcag tcgaccccga
     5221 cccaggatga actggagatc gaccacccgc tctccttctg ttccggaccc tcgttcggga
     5281 agccctgggt gatcagcggc gacaccggga tccacccgag tgccgccggc tacctgcaga
     5341 tgtccagcca gattcccgcg cccgggtcct gaagctcgcg aaccgggaag ggcacccgag
     5401 tagcatcggt tccatgttct tcggtcgcga caaaaccacg atgcccgccc cggaagattc
     5461 actcccgggc cgctccggct acgccttccc ggttccggac aatcacaccg ttctgggaac
     5521 cccgatccag ggacccttcc cggaagggat cgaggtcgcc catttcgcgc tcggctgctt
     5581 ctggggcgcg gaacggcttt tctggaacct cgacggtgtc tacacgaccg cggtcggcta
     5641 ccagaacggg tttacgccct tcccgaccta cgaagaggtc tgctccggga agaccggcca
     5701 cgccgaggcg gtgctggtcg ccttcgatcc ggagaaggtc agctacgagc ggctgctcga
     5761 ggtcttcttc tccgagcacg acccgaccca ggggatgcgt cagggaaatg acgtcggaac
     5821 ccagtaccgc tccgccgtct tcacggtcgg cgaccggcag aaggagatcg ccgagatggc
     5881 ccgcgacacc tatcagcggg cactgaacga gcggggtctg ggcgagatca cgaccgaaat
     5941 cgaagaggcc ggccccttct attacgcgga gggcccgcat cagcagtacc tggacaaggt
     6001 cccgaacggc tattgcggcc tggctggcac cggggtcgca tgccaggtgc cggtggccgg
     6061 cggttcctcc acctgagccg ccggggatcc gccgggaacc gtttcgcccg gccataaatt
     6121 aggattcccg cggtgccaac cctgctcaca ggacggcctt gaccccgtcc ctagcctcat
     6181 acatcgccgg aacgctccag atcgccgtcg tggtcggcgc cgtctggtac ggatcctggt
     6241 ggcttcggcg atggatcgtg cccgaattct cgggtgcgct ggcgcgcctc gccgagctga
     6301 cgatcgcctt cgcgctgatc atcctctcgc tcgagctgat cggttcggtc ggcttgctga
     6361 aggaggtctg gatcaccgtc gcctgcgtcg tgatccccct gatcggcgcc ggcctggcct
     6421 ggaagttcgc gccgcgagac gttgaggaga ttgccgcgcc agcggtcccc acctgggcct
     6481 tcctggtcgc ggtcgcggtg gcgtcctggt gtgtggcgga atgggctttc ccgacccagc
     6541 tcagcctcga ccagggcatg ttcggtggtg acacgacctg gtaccacatg ccggccgcgg
     6601 cgaggttcat tcaggactcc tcgatcatcc cgctccactt caccgacgcg ctgcggctgg
     6661 cggtctggtt ttacccccag aactcggagc tgatgcatgg cgccctgatg ggcgtgatgg
     6721 gcagcgactg gctttccccg gtcttcaaca tgctccccct cgggatcgcc ctgctcgcct
     6781 gctggtgcgt gggccggcct tacggggtag ggcccgccac cctggttgcc ggcgcgatgc
     6841 tcctttcggc cggggtgatg atcgagaccc agcccggcga gggtcgaaac gacatcgtct
     6901 gcttcgcctt cctgatcgcc ttcgtcgcct tcctgatcaa cgggcaccaa aggcgggcgc
     6961 ccagttccgg agcggtggac gaaaagcccg acccgaacgc tcctctgctt gacaagggcc
     7021 cgctgatcct cgccggtatt gcggccggca tcgcgatctc gatcaaggtt tcgatgctgg
     7081 cgccggtcgg cgtgatcttc ctcggcatga tcctgatcag cggcaaggga cgccgggtca
     7141 cgaccgcgct ctgcctcggc atctcgatgt tcatcgtcgg cggctactgg tacgtgcggg
     7201 cgatgctcta caccggcggc aacccggtgc cggccgtcgg ttggggcccg ctccacctgc
     7261 cgcagccgga ccagatgccg ctcgacccgc gcccgcggtt ctcggtcgcg cactacatca
     7321 ccgacccgag catctaccgc tggtggttct ttccccggct cgatgacgcc ttcggggtgc
     7381 tgttcccgct gatcctggtc atgctcgcag cggccgcgct ctggctggtc ttcaagtccc
     7441 gcaacaagat cgtccgggtg atctccgcgg ccgccctgat caccgccttc gtctatgtgt
     7501 tcaccccgct gaccgcggcc ggccaggagt tccagccgcg cggtttcttc accaacacca
     7561 ggtacctgtt gccggggctc cttctggccg cggtgatgct gccgctgatc cgccagttgc
     7621 gtgagccgca ggcgagggcg aagaagaccc tgatcttcct caccgtggtc tacgcgatca
     7681 cggtgctgac ctcgcccaag tggtacccga gcttcctgcc cggcgccatt ttcctcaccc
     7741 tggcgatcgt ctgggccccg gtcggcctga gctggatgaa ggacaacacc agggtgccga
     7801 agtacgtgat cgtgggctgc gtcgccgcga tcgcgatcat cggcgcgatc tggggccgtg
     7861 gtgaagaggt cggttacgcc gacaagcact acacccggac cactctcttc ctgcaggaag
     7921 gcggccccca ggacgccttc gaattcaccc ggaacctcaa ggacaagcgg atcgcgctcg
     7981 ccggttcggg tgagatcttc ttcggccagt acggcttcta cggggtcgat cgctcgaacc
     8041 acgtccagta catcggtcag gagggtgaca acggcaccta ccgtttgatc accaactgtc
     8101 gtgaattcat caacacgatc aacgaaggtg actacgacta cctgatcacc agcgagtaca
     8161 cccaggacgc gccggaagcc gattactggt tcccggtccg ggcctgggtt gacggtgacc
     8221 ccaacgcgga gctcgtcgtc tcggaagacg acatcactcc gcagccggat tacgtctaca
     8281 agatcaacgg caagctcgat ccagaccgct gcgccaagct cgaccaggca gcgatccgcc
     8341 aggtgcggac cgacgcgctc caggcgatca aggatcgtga ggcagaagcc gcggccgcgg
     8401 aagccgccgc agagtcatcc gattcgcagt agccgcttcc gcccggccag cggcgggaat
     8461 ggctagcgtt accttttcgc gcccccgtag ctcagctgga tagagcgccg gccttctaag
     8521 ccggatgtcg caggttcgaa tcctgccggg ggtgccttcg tcccgctgtg aagaaggcga
     8581 tgatcgtgac aatcaggatc aggctgccgt agacgagggc ggtggcgtgg aggccgtacc
     8641 ggtccgaggc gaagccgccg atcagggcgg ggacgctgaa ggcgatgtag ctgaccacgt
     8701 aggcgaaggc gaagagctcg ccacgttcgt cgggatgggg gagggtggca agtacgccga
     8761 aggaaccgag cgccgaggct ccgaagccga ttccggaaat cagggtcccg gcaagggcga
     8821 gcggggccag gtcgagcagc aggccgatca aattgatcgt gattccggcg gccagccagc
     8881 ctgccgcgaa gcggatcacg gtcaccggct cgcggtgacg gagccgatag acaaccacgg
     8941 ctccggtccc gcagagtgcc gtgaccacga tcgcgccggt caggtagggg tcatcctcga
     9001 tgaagctcga tgcgatcgaa ggacccagtg acagatagag cccggccagt gcccagccgg
     9061 cgatcatgat cgggagcagc gagaagaacc ggggcctgag gtgggccggc aggccgacct
     9121 tcggtaacag tgactcgcgg gcgcccggtt tcatggtcga ggtttcgggc gagcgccaga
     9181 cagcgaccgc ggcaaacccc agcgccgcca gcaggatcag gaagatcagg gtggtcggcc
     9241 agggggcgaa ctggatcagc acgccgcagc cgagcgcacc gatcgccagg ccggcgagcg
     9301 gcgccactcc gttcaccacg ccgcttcgct tcgggtcatc ctcgggctgc aggtcgacga
     9361 tcaccgcgct gagcgtggcg agcgccaccc cggtggcgag accctggatt accctggcca
     9421 gggccagcat cgcgactccg ccggaaagca ggaacagcag gagggaaagg gcctcgaagg
     9481 caatcgctcc gagcagcacc gggcgcctcc cgaggtgatc ggagagtgac ccgaagacga
     9541 gcagggagca gagcagcgcg accacgtata cggcgaagac ctcggtcagg accgcgtcgg
     9601 agaagcccca catgtcctgg tagagggagt agagcgggga aggcaccgcc gagatcgcgg
     9661 ggaaggtcac ggcgatcgcc gccatcgccc agaaggcgcg ttcgtggggg atcggattgc
     9721 gggatgaacg gaagcgtgaa ggaggcatca gggcgagtca gggggagaca ggcaaggtac
     9781 caacggcacc tcttgagcaa ccgcgcactt gcctgacatc cggaagacga catttcgtta
     9841 cggaccggaa ttgacatttg aaacccgtcg tttggttata gtcatgaatg ccggtctcat
     9901 gacgatgtct ggcaggcaca tgacaatcgc caacaggaga gactatgtcc agcattgaag
     9961 acgcaattca acaacttgaa acttcagctt cgaagctcaa gcagctttcg gttgaagaaa
    10021 gccgtgcgat tcgcgaagcg gtcagggttg caaccaggga agcaaccgcg aaggtaaagg
    10081 ccgagtacaa ggacaagaag gacaaggccc gcaaggaagc cagggacgcc gagaagtcaa
    10141 tcaaggacgc ccagtccagg atccagaaag ctcttggttc ggaaaagggc gggtcgaccg
    10201 gcggcaagcg cgccaagcgc ggcgagcggg aagcccagtt cgtcgcatac gtgaaggaga
    10261 accccgattc gaagctcgcc gacatcgcgc ggggaatcgg tgtgcagaac agcgccgcca
    10321 acgggctggc caagaaggca gtcgccgccg gcaaggtgaa gaagagcgcc cagaagacct
    10381 acaaggccgc ctgatcactt tcagagatgt tttgaagggc ccgctccggc gggccctttt
    10441 tcgtgtttca gcccttagaa tcgaccggta atgcggctcc cgggagggcc gtgacgagca
    10501 agaggagaga taaatgaacg ttgttcgcag gggatcgctg gcctttctgg caacagtggc
    10561 ggcgctaggc attttcatgt cgggaagcgc ttcggccaag ctgaacgtaa tgaccgttca
    10621 gggcacgccc gcgccgggca caccggcgaa gtacaacaag ttcaaggtcg taaagcaggg
    10681 gccggccaag gcaaagaaga tcctcctcct cgagcccggc acttcggccg gtgccaccaa
    10741 cttccgcccc ctcggtaacg ccctggtcgg tcaactcaag ggctggcagg tctggtcgat
    10801 cgagcgtcgc gagaacctgc tcgaagacca ctcgatgctg gagaagtacc tcaagggtga
    10861 tgcgaccaac gaggagatgc tcgactacta cctcggctgg atcctggacg gctcggttca
    10921 gcctcatttc gagggcaaga ccaccgcgga aacccagtac gcgaagaagt ggggtctgag
    10981 catagtcgtt cacgatcttc gcaaggtcgt gaacatggcc cgcaaaggcg gccgcaaggt
    11041 cgtgcttggc ggccactcgc tgggcggttc gatcgtcacc gcctacgcga cctggaattt
    11101 caacggaacg ccgggcgcca agggactttc cggcctggtc ttcatcgacg gtgccggggc
    11161 ccgcgaggcc gcggacctgc ccacggccgg ggaagcgcag gccaacctcg acagcttgaa
    11221 cgccgaaagc gcctcgccct tcatcacgct ggtgccgccg ctgccctggg cggcaggggt
    11281 cttcaacgcg atgggttcga ccgcggcgct tcaggatccg gattcgcgct cgctgatgga
    11341 ggatttcccg ctgctcccgg attcgctgaa gccgcccgtt tcggcgacca acctggccca
    11401 gtacgcatac gcgatcgacg ccgacaccgg cccggagaat ctgcagctgg tccagagcca
    11461 cgtcggtgac ctcgcggatt ccggtgaccc ccggggctgg gtcaacggcc agctcggcac
    11521 cgcccagcgg gccgcccagg tcttcaccga gatcgacggc atggacggca cctcctggta
    11581 tcacccgctg cggctgagca tcgacgccgg tgcgatcaac aacgggatcg ccaccccggc
    11641 ccagcaggtc tacggaatca agacgaccat gggcaagcgg gttcacctgc cgatgtactc
    11701 gtttgacacc tcgctcggcg acggccgggt caagcgggcg accgatgcgc ttgcggccca
    11761 gtcaaaggtg ccgaagagct gggtgcggac ggtggtccgg aaagagacct acgcccacat
    11821 cgacccgctc agcgccaccc cgtcgaagaa cgacttcatc aagacgctgg tcccgttcct
    11881 gaagaagcag gtcaagtagg ggccgccatc tggattgaag ttatccgcac atagagcgaa
    11941 taacttcaat ccagttgcag cctcagtcga agatgacgat ctggcggacc gcctcgcccc
    12001 gggcgaggcg gtcgaagccc tcgttgatct gatccggtgt gagccggtgg ctgagcagct
    12061 tctcgaccgg caactcaccg tcccggtaca tgcgaatgaa cgcggggatg tcccggcggg
    12121 gcacgcagga gcccaggtac gagcctttca gggtccgctc ctcggcgacc agtgagatcg
    12181 ccgggatctc cagcttgcgg ctggggtggg gcagcccggc ggtgaccgtg gtgccgccgc
    12241 ggccggttgc cgcgtaggca tcggccagca cgttctcgtt gccgaccgtt tcgatcgcga
    12301 ggtcgacgcc gccgccggtc gcttcgcgga tcgaatcgac cagatcgggc ccggcctgaa
    12361 tggtcaggtc cgctcccagc tcgcgggcca gctcgagctt ggccggcacg gtgtcaaccg
    12421 cgaccagggt ttccgggttt tgtgacttcg cggcaagcag cgccgccagg ccgaccccgc
    12481 ccagtccgaa gacggcgacc gatcgggccg gcaaagcggc ggcacccgtg ttgagcaccg
    12541 cgccggcacc ggtcaggacc gcgcagccga aaagggccgc gatgtcaaag ggaaggtcat
    12601 ccggaacctt gaccgccgag cgggccgaga ccacggcgtg gtcggaaagg gcggaaacgc
    12661 cgaggtgatg gtggacctcg gtgccgctct cctcgaacag gcggatgtga ccacccagca
    12721 gggttccctc gacg
//
LOCUS       DAOQKN010000058        12700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_2312_length_12700_cov_5.745670, whole genome shotgun sequence.
ACCESSION   DAOQKN010000058 DAOQKN010000000
VERSION     DAOQKN010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 12700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12700
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2312_length_12700_cov_5.745670"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1683)
                     /locus_tag="PLJ64_05495"
     CDS             complement(<1..1683)
                     /locus_tag="PLJ64_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92972.1"
                     /translation="MNHSSATLRRPVLLAIALVTALTMLAAWSASSRAAVICVPDAGV
                     SPDCQTSFATIQPALDSAKNSGGNDEIRLGTGSFSGDLSYDNTTAGNTLDIIGSGQNP
                     GQTVLNMVDTAGNNEGFHFTSVPGSTIRNLTYRIPIAADGSSDVGLNLNGVTADGIVV
                     DGPTASQVRAIACSTCNLTGSTIDLPIENGTIGVDQTGGPAYVGDSVIKADYGVRHSS
                     PGLTTTVERSRITAHTGASTDGGNLEIFNTLILLDDQPNAVGINLYNPNEGTYAIGAT
                     LDHLTIISGGGQHSIGIRAIADSESSGEGESVDATLTNSVIHGFNGTGEHTLDLLADK
                     GRTVTFHSSYSAYDNARTSNVTGSEGGTVDYELSPGHLDLEGGVSFVDPQSGDYTPSL
                     LGGLLDGGDPIGPLTGITDINGNPRACHGTDEGIIRQDIGAFEARTVVSSEPDDCTYP
                     VTSILAKPPASTTAKTASFTLGTDKNPGTFLCSLDGGPESDCGYTFETPTLSVGDHSL
                     SVRAKDQFGNIDQSPETYSWSVKDSGPGPCETNCVDKTAPKVIGVKAPKQTRA"
     gene            complement(1708..3006)
                     /locus_tag="PLJ64_05500"
     CDS             complement(1708..3006)
                     /locus_tag="PLJ64_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92973.1"
                     /translation="MTLVDGATLKDSAVRLYSSNVNDAVDVYGSGNVVDSTLTAYRGV
                     YQMNSTGTVEIKRSALIPIDGGAVTEGGTMNIRDSVIINLNGGRGVFVDSYRGPAHVL
                     IDGSTILGAPGSTGAEVHARRDQDDNPGTTSFKVNNSIIYGQDFAIVYHEYFAEDTLT
                     VDVDSSAYDSSRVVEQPGAGTPQFTTKDPVELNGVDPMFADPDHGDYSPDAGSPLIDA
                     GQAALPPVGSLAYDRHPRACDGNDDGTIRRDIGAWEYRSDPSDDCTYSKATINGPRGA
                     ATARSRSFTFKASKPGSTYLCSLDGAAFAACTTPYTASGLAVGNHSLKVKAKDSYGNV
                     QTPPTEKSFKVEAADKTAPKVIGVKAPKQTRAATVKVSFKSNEKGSTFKCRLGKGKWK
                     SCKSPWKTPKLRKGRNSISIQATDRAGNRSKVKTVAVKRK"
     gene            complement(3003..3518)
                     /locus_tag="PLJ64_05505"
     CDS             complement(3003..3518)
                     /locus_tag="PLJ64_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92974.1"
                     /translation="MKRALIFMAIGLASIAAFGATQAQANTYCAELPADASCDQNFTG
                     TGTSIQAAVEAAADNPGPDSVKVGPGNFVMPSPVSLSSPGDANKLTLTGSGPSTVLSG
                     DDPQLPDINFSGGTGSSINDLTVSISDDASNEYKVGLALGAGATGSRLTVTFASHLVN
                     GDPSPGKKLLA"
     gene            3590..6136
                     /locus_tag="PLJ64_05510"
     CDS             3590..6136
                     /locus_tag="PLJ64_05510"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNC92975.1"
                     /translation="MDDAAKKKLEETTRWEPGETEPRIFAEWLEGGYFHPEATGTADQ
                     NYSVAIPPPNVTGVLHMGHALNGSMQDALVRMNRMSGRNTLWILGMDHAGIATQTVVE
                     KRLKSEGISRHEIGREAFTERVWEWKEEFGGSIREQYKRLGASVDYERERFTLDDGYA
                     RAVRKVFTSLYEKGYIYRDHYMVNWDPGSQSAISDLEVVNEEVTDHLYSVDYPVEGSD
                     RVLTVATVRPETMMADTAVAVNPKDERYSDLIGQHCVLPLVGRRLPIIADDHVDVEFG
                     TGALKITPGHDVNDFEIGRRHGLEEISVIGEDGRMTAEAGERFEGMTVAEAQVEVVKA
                     LKEEGALSGEEEYVHSVPFSERSGERIEPLISLQWFCQMDELARPAIEAVEDGRVNIA
                     PGQWKRVYLDWMREIRPWCVSRQLWWGHRIPVWYRGNETYVGETEPEGEGWVQEEDVL
                     DTWFSSAIWPFATLGWPDETDELKAFYPTDFLTTAREILFLWVARMIMTGLEFAGDVP
                     FHDVYVHSVIQARDGRRMSKSLGTGIDPLEEIDEHGADALRFGLLAMSSTQDVRYSDP
                     KVQQGRDLATKLWNASRLILLNTEDPADRTVPAAPICLEDRWILSRLETTIETYTRLI
                     GEYDFAHAAQELYSFVFSDLCDWYLEIAKPRIYDGDPEISQVLLHTLERTLALAHPVM
                     PFVTEAIWEYHPYRQGHLVVHPFPVPDSSRISEAVETEVGEAIGVTRQLRGWRDMAGV
                     PPKVVLDAAGDPGSVPEFVPRLGRVNIGAGEGETVATVAGFGILASDELDMEAVTARI
                     GARRGKLEAELKKIEGKLANPRFVENAPAEVVAEEREKVGRLQSELAELG"
     gene            6144..7424
                     /locus_tag="PLJ64_05515"
     CDS             6144..7424
                     /locus_tag="PLJ64_05515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNC92976.1"
                     /translation="MTGPADEAPASPFDSEAFLNSLEPIGWKLGLERMEMLSDELGRP
                     QDSFATIHVVGTNGKSSVSRMIAAICEAHGFRAGCSLSPHLAHWSERVAISGEERPGL
                     FADCVRRAAEAAASVNARLEDEEVTQFELATAAAFLTLAEAGVQVAAIEAGLGGRLDA
                     TNSINSLATVLTSIGLDHTEYLGETEVEIAGEKLAVLRPATTLVLGPVSPAVRSLARE
                     IAAGRDCDLREISDAATTGATGIEGIGGFQRTNFAVAEAAAACFLGEVSRRKSEEAVG
                     GLVIHGRLEMIGQDPPVFADVAHNPAGARALAASLPEVTGDLPVIGVIGVLADKDAPG
                     MLAELAGSLEAAVFTGLPGEALAAWGRPGARAWDPEELQGLAGRLGLEAETARTPAGA
                     LERAREMALEKGGAVIITGSHFLLAPGEGAGFQG"
     gene            7619..8389
                     /locus_tag="PLJ64_05520"
     CDS             7619..8389
                     /locus_tag="PLJ64_05520"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNC92977.1"
                     /translation="MPIAIFGIGNTALNLAVTLAILMLVAIWIALIVFTFTDARRRIA
                     DPFLVGCATAASFFPFVGTLVYTILRPPEFLEDIRERETDITESEVRLRHLKHSSCHK
                     CGSPVESDFIRCPACRSRLKEPCPTCSKPVGLNWKVCPYCETTLIAPKRSSRSSGSKR
                     PKGESSGRPSSSRSGRSERAPEGKEERRRRSSKAPAESGARESRSSSGESRPEDSSRR
                     SGNSSSDQADSSRSPRSARRKISVSDDEKSEPINRGDS"
     gene            8481..8888
                     /gene="ndk"
                     /locus_tag="PLJ64_05525"
     CDS             8481..8888
                     /gene="ndk"
                     /locus_tag="PLJ64_05525"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNC92978.1"
                     /translation="MSQTLILVKPDAFERALTGEVLARFERKGLRIAALKKLTATEQI
                     ANEHYAEHKEKPFFGELVEFITGGPLVAAVLEGPEAVVAARQLIGATNPVEAAPGSIR
                     GDFALEVTFNMVHGSDSDESAAREIAIWFPEGI"
     gene            9309..9872
                     /locus_tag="PLJ64_05530"
     CDS             9309..9872
                     /locus_tag="PLJ64_05530"
                     /inference="COORDINATES: protein motif:HMM:NF037470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QsdR family transcriptional regulator"
                     /protein_id="HNC92979.1"
                     /translation="MGVSEKVRRDDAIRLARRHYLNLERVDMQSLANELGIGRTTLYR
                     WVGDRDSLISEMLSEMVVEVIGTAIGEAEGEGLERTIDAMRRFMLTTADFAPLRHLVQ
                     NEPELGLRVMLAPGSGVSTAITGELTAEIERTRPEWSGEKASELADVLTQIGMAYEWG
                     NIAVRSEPEIDRAIRAMETLVRAADTA"
     gene            9905..10471
                     /locus_tag="PLJ64_05535"
     CDS             9905..10471
                     /locus_tag="PLJ64_05535"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HNC92980.1"
                     /translation="MPACPVILASGSPQRRDLLERIGVEFEVRVSGVEEIVHGDPAEV
                     VLANALLKARSVAETAPGCLVIGCDTDVVLEDHIIGKPDDEVRAREYLAQLSGRKHRV
                     LSGLAIVGPEAEQVRTGVAESAVRFRELEKSEVDRYLASGEWRGRAGGYAIQGRGSSL
                     VAGVDGDIANVIGLPVGLLLDLAPELDL"
     gene            10681..11496
                     /gene="mreC"
                     /locus_tag="PLJ64_05540"
     CDS             10681..11496
                     /gene="mreC"
                     /locus_tag="PLJ64_05540"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNC92981.1"
                     /translation="MLIGLIVLGFVLLTFTFGSGSGGVGGVLGTFFDPVTNAASRALK
                     PARDLINWFDETMDARGERDRLQSELEEARGKAVAGEVALEDNRQLRKMVGLAEAGKI
                     PDAYDPVTVNVTGRSPTVWYTTVSVNAGSSDGIAVNDPVISGDGLVGRISAVSHSGSV
                     VSLISDGSSAVSARVLPGGAQGVVRAKVGDPQTLVLEFLDETKDVHSGDTVVTSGWRG
                     DGLSSLYPRGLPIGEITRAPIDEREAIQSVELRPFADLRNLDWLQVLTGGNRG"
     gene            11493..12086
                     /gene="mreD"
                     /locus_tag="PLJ64_05545"
     CDS             11493..12086
                     /gene="mreD"
                     /locus_tag="PLJ64_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HNC92982.1"
                     /translation="MIITSRTAARVAGLILLFTVLQISFFSQIELFGTSMWILPACAA
                     IFGLLGGSLIGATVGFAFGFLGDGLTDGPLGSACLVFMAVGYLAGIYRERGDFVDRPT
                     VLAVCGIATLGANLALGFYTVIVGFDSSLSGSLVPDLILQAFYGVLLAIPLYALTYRV
                     LRPALIHEPATRRRPVDIERWADELDPPGGNGPEAFR"
     gene            12131..>12700
                     /locus_tag="PLJ64_05550"
     CDS             12131..>12700
                     /locus_tag="PLJ64_05550"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92983.1"
                     /translation="MSRRFAVIGGIGLVLLGILFFRLWSLQIVTGDKYLAEANDNRTR
                     EIRVPAPRGRILDRDGKVLVDNRTSMALQLDPIEIPTTVAGRQELYARIGKVLDHSAD
                     WIRKKVYETKKNDPPGSPITLDQDVDDDIVYYLQEHQAEFPEVQVNRIFVRKYPQGLL
                     AAHILGSIGEVTAEDLEKNPDKDLAAGDTL"
BASE COUNT         2078 a         4077 c         4266 g         2279 t
ORIGIN      
        1 ggccctggtc tgtttcgggg ccttgacccc gatcaccttc ggggcggtct tgtcgacgca
       61 gttggtctcg caaggtcccg gaccggagtc cttgaccgac caggagtagg tctcgggcga
      121 ctggtcgatg tttccgaact ggtcctttgc gcgaacggac aggctgtgat cgccgacgct
      181 gagggtcggg gtctcgaagg tgtagccgca gtcgctttcc gggccgccgt ccagtgagca
      241 gaggaaggtg cccgggttct tgtcggtgcc cagggtgaag gaggccgtct tcgcggtggt
      301 gcttgcggga ggcttggcaa ggatcgaagt caccgggtag gtgcagtcgt cgggctcgga
      361 gctgacaacc gtccgggcct cgaaggcgcc aatgtcctga cggatgatgc cttcgtcggt
      421 cccgtggcag gcacgagggt tgccgttgat gtcggtgatg ccggtaaggg ggccgatcgg
      481 gtcgccgcca tcgaggaggc cgccgagcag ggagggagtg taatcgccac tctgcggatc
      541 gacgaaggaa acgccgcctt cgaggtcgag gtggcccggc gaaagctcgt agtcgaccgt
      601 gccgccctcc gatccggtca cgttcgatgt cctggcgttg tcgtaggccg agtaactcga
      661 atggaaagtg accgtccgtc ctttatccgc caaaagatca agcgtgtgct cgccggttcc
      721 gttgaagccg tggatcaccg agttggtcag ggtggcatcc acactttcgc cttcgccgct
      781 cgactcgctg tcggcgatcg cgcggatgcc gatgctgtgc tggccgcccc cgctgatgat
      841 cgtcaggtgg tcgagggtgg cgccgatcgc ataggtccct tcgttggggt tgtagaggtt
      901 gatcccgacc gcgttcggct gatcgtcgag aaggatcagg gtattgaaga tctcgaggtt
      961 gccaccgtcg gttgaagccc cggtgtgcgc cgtgattcgg cttctttcga cggttgtggt
     1021 tagacccggg gaactgtgcc tgaccccata gtcggctttg atcacggagt cgccgacata
     1081 cgccggtcct ccggtctggt cgacgccgat agtgccgttc tcgatcggga ggtcgatggt
     1141 cgagccggtc aggttgcagg tgctgcatgc gatcgcccgg acctgggagg cggtcgggcc
     1201 atcgacgacg attccgtcgg cggtcacgcc gttgaggttg agaccgacat ccgagctgcc
     1261 atcggcagcg atgggaattc tgtaagtcag gttcctgatc gtcgagccgg ggaccgaagt
     1321 gaagtggaag ccttcgttgt tgccggccgt gtcaaccatg ttcaggacgg tctggccggg
     1381 gttctgcccg gagccgatga tgtcgagcgt gttgccggca gtggtgttgt cgtaggagag
     1441 gtctccggaa aagctgccgg tgcccagtcg gatttcatcg ttgccgccgc tgttcttggc
     1501 tgagtcgagg gctggctgga tggttgcgaa gctcgtctgg caatccgggc tgacccctgc
     1561 gtccgggacg cagatcaccg cggccctgga gctggcgctc caggcggcca gcatggtgag
     1621 cgcagtgacc agggcgattg cgaggagaac gggacgacga agagtggctg aagaatggtt
     1681 cattggaatg gagataggaa cctgtgttta tttgcgcttg acggcgactg tctttacctt
     1741 cgacctgttg cctgcccggt ctgtggcctg aatgctgatc gagttcctgc ccttgcggag
     1801 cttcggggtc ttccagggtg acttgcagct tttccacttg cccttgccga ggcggcactt
     1861 gaaggtcgag cccttctcgt tcgacttgaa gctgaccttg accgtggcgg ccctggtctg
     1921 tttcggggcc ttgaccccga tcaccttcgg ggcggtcttg tcggccgcct cgaccttgaa
     1981 cgacttttcg gtcggaggcg tctggacgtt gccgtaagag tctttcgcct tgaccttcag
     2041 gctgtgattg ccgaccgcca gtcccgaagc cgtgtacgga gtggtgcagg ccgcgaatgc
     2101 ggcgccgtcc agcgagcaga gataggtcga gccgggctta gacgccttga aggtgaagga
     2161 ccggctcctt gcggtcgcgg ccccccgggg gccgttgatg gttgctttcg agtaggtgca
     2221 gtcgtcgctc gggtcggacc ggtactccca ggccccgatg tcccggcgga tggttccgtc
     2281 gtcgtttcca tcgcaggcgc gggggtggcg gtcgtaggca agcgaaccaa caggaggaag
     2341 ggcggcctgg ccggcgtcga tcagcggcga cccggcgtca ggcgagtagt cgccgtggtc
     2401 ggggtcggcg aacatcgggt cgaccccgtt cagttcgacc ggatccttgg tcgtgaactg
     2461 gggcgtgccg gcgccgggtt gttcgaccac cctggaggag tcgtaggccg aggaatccac
     2521 gtcgacggtc agggtgtcct cggcgaagta ctcgtgatag acgatggcga agtcctgccc
     2581 gtagatgatc gagttgttga ccttgaaact ggtggttccg gggttatcgt cttgatcccg
     2641 tcgggcgtgg acctccgctc cggtcgagcc gggtgcgccc aggatcgtcg aaccgtcgat
     2701 cagcacgtga gccgggcctc tgtagctgtc cacgaagacg ccgcgcccgc cgtttagatt
     2761 gatgatcacc gagtcacgaa tgttcatcgt gccgccttcg gtcacggcgc caccgtcgat
     2821 cgggatcagg gccgatctct tgatctccac cgtgccggtt gaattcatct ggtagacccc
     2881 gcggtaggcg gtcagcgtcg aatccaccac gttgccggac ccatacacgt caactgcatc
     2941 gttgacgttg ctgctgtaga gcctgacggc cgagtccttg agggtggccc cgtccaccag
     3001 cgtcacgcca gtagtttttt gccagggctc gggtctccgt tgacgagatg cgaggcaaag
     3061 gtgacggtca gcctgcttcc ggtcgcgccg gcacccagcg caagaccgac cttgtattcg
     3121 ttgcttgcgt cgtcggaaat gctgaccgtg aggtcgttga tgctcgagcc ggtgccgccg
     3181 ctgaaattga tgtcgggaag ctgcgggtcg tcgccggaaa ggaccgtgct gggtccggaa
     3241 ccggtcaggg tcagcttgtt ggcgtccccg gggctcgaga gggagaccgg acttggcatc
     3301 acgaagttgc cggggccgac cttgaccgag tccggccccg ggttgtcggc ggcggcctcg
     3361 accgcggcct ggatcgaggt gcccgttccg gtgaagttct ggtcgcagct cgcgtcagca
     3421 ggaagctcgg cgcagtaggt gtttgcctgc gcctgggttg ccccgaatgc cgcgattgac
     3481 gcaaggccga tcgccatgaa gatgagtgcc cgtttcatca aaccttcccg gtaagacagt
     3541 gaagtaccga acaccctaac ccggtggccc gtcaataaga tcggattcct tggacgacgc
     3601 ggcaaaaaag aagctggaag agaccacccg ctgggagccc ggcgagaccg agccgcggat
     3661 ctttgccgag tggcttgaag gcggctactt ccatcccgag gcgaccggca ccgccgacca
     3721 gaactactcg gtcgcgatcc cgccgccgaa cgtgaccggc gtgctccaca tgggtcacgc
     3781 cctcaacggc tcgatgcagg acgccctggt ccggatgaac cggatgagcg gcaggaacac
     3841 gctctggatc ctcggcatgg accatgcggg catcgcgacc cagacggtgg tcgagaagcg
     3901 gctcaagtcg gaggggatca gtcgccacga gatcggccgc gaagccttca ccgagcgggt
     3961 ctgggagtgg aaggaagagt tcggcggttc gatccgcgag cagtacaagc ggctgggtgc
     4021 ctcggtcgat tacgagcgcg aacgcttcac gctcgatgac ggatacgccc gtgcggtccg
     4081 caaggtcttc acctcgcttt acgagaaggg ctacatctac cgcgaccatt acatggtcaa
     4141 ctgggatccc ggttcgcagt cggcgatctc cgatctcgaa gtcgtaaacg aggaagttac
     4201 ggatcacctc tattcggtcg attacccggt cgagggaagc gaccgggtgc tgaccgtcgc
     4261 caccgtccgt ccggagacga tgatggccga caccgcggtc gccgtgaatc cgaaggacga
     4321 gcgctacagc gacctgatcg gtcagcactg cgttctgccc ctggtcggac gccggctgcc
     4381 gatcattgcc gacgaccacg ttgacgtcga gttcggcacc ggcgcgctca agatcacccc
     4441 cggccacgac gtcaacgact tcgagatcgg ccgccgtcac gggctggaag agatctcggt
     4501 gatcggcgag gacggccgca tgaccgccga agccggcgaa cggttcgaag gcatgaccgt
     4561 ggccgaagcc caggtcgagg tggtcaaggc gctgaaggaa gaaggggccc tgagcgggga
     4621 ggaggagtac gtccattcgg ttcccttctc cgagcgctcc ggcgagcgga tcgagccgct
     4681 gatctcactt cagtggttct gccagatgga cgagctggcc aggccggcga tcgaagcggt
     4741 cgaggacggc cgcgtgaaca tcgcccccgg gcagtggaag cgggtctacc tggactggat
     4801 gcgggagatc cgcccctggt gcgtttcccg ccagctctgg tggggtcacc ggatcccggt
     4861 ctggtaccgc ggcaacgaga cctatgtggg cgagaccgag ccggaaggcg agggctgggt
     4921 ccaggaagag gacgtgctcg acacctggtt ctcctcggcg atctggccct tcgcgactct
     4981 cggctggccg gatgaaaccg acgagctgaa ggccttctac ccgaccgact tcctgaccac
     5041 cgcccgggag atcctcttcc tctgggtcgc ccggatgatc atgaccggcc tcgagttcgc
     5101 cggcgacgtt cccttccatg acgtctatgt ccattcggtg atccaggctc gcgacggccg
     5161 ccgcatgtcg aagagcctcg gcaccggcat cgacccgctc gaggagatcg acgaacacgg
     5221 ggcggacgcc ctgcgcttcg gcctgctcgc gatgtcctcg acccaggatg tccgctactc
     5281 ggacccgaag gtccagcagg gccgcgacct ggcgaccaaa ctctggaacg cctcccgcct
     5341 gatcctgctc aacacggagg accccgccga ccgcacggtc ccggccgcgc cgatctgcct
     5401 ggaggaccgc tggatccttt cccgcctcga gaccacgatc gagacctaca cccggctgat
     5461 cggggaatac gacttcgccc atgcggccca ggagctctac tccttcgtct tctccgatct
     5521 ctgcgactgg tacctggaga tcgccaaacc gcggatctac gacggtgatc cggagatcag
     5581 ccaggtcctg ctccacaccc tggagcgcac cctcgccctg gcccatccgg tcatgccctt
     5641 cgtgaccgaa gcgatctggg agtaccaccc ctaccggcag ggtcatctgg tcgttcatcc
     5701 cttccccgtc ccggattcct cgcggatctc cgaggcggtc gaaaccgagg tcggcgaggc
     5761 gatcggggtg acccggcagc tgcgtggctg gcgcgacatg gccggggtgc cgccgaaggt
     5821 cgtgctcgat gcggccggtg atccgggatc ggttccggaa ttcgtgccac ggctgggccg
     5881 ggtcaacatc ggcgccggtg aaggcgagac ggtcgcaacc gtcgccggct tcggcatcct
     5941 cgcctcggat gaactcgaca tggaggccgt gaccgcgagg atcggcgccc gtcgcggcaa
     6001 gctcgaagcg gagctgaaga agatcgaagg caagctcgcc aacccgcgct tcgttgaaaa
     6061 cgctccggcg gaagtggtcg cggaagaacg cgagaaggtc gggcggcttc agtccgagct
     6121 ggccgaactc ggctgaggcg cccgtgaccg gccccgccga cgaggccccc gccagcccct
     6181 tcgattccga agcgtttctc aactcgctgg aaccgatcgg ctggaaacta gggctggagc
     6241 ggatggagat gctctccgac gagctcggcc gcccgcagga ctcgttcgca acgattcacg
     6301 tggtcggcac caacggcaag tcctcggttt cgcggatgat cgccgcgatc tgcgaggccc
     6361 atggcttccg ggcaggttgc tcgctttcgc cgcacttggc tcactggtcc gagcgggtcg
     6421 ccatttccgg cgaggaacgg ccggggctgt tcgccgattg tgtgcggcgt gcggccgaag
     6481 ccgcggcaag cgtgaacgcc cgtctcgagg atgaggaggt gacccagttt gaactggcga
     6541 ccgcggccgc tttcctgacc ctggccgagg cgggcgtcca ggtcgcggca atcgaggccg
     6601 gtctcggcgg ccggcttgac gcgaccaact cgatcaactc cctggccacc gttctcactt
     6661 cgatcggtct cgaccacacc gagtacctcg gtgaaaccga agtagagatc gcgggggaga
     6721 agctggccgt tctgcggccg gcaacgaccc tggtgctcgg tccggtctcc ccggcggtgc
     6781 ggagcctggc ccgggagatc gccgcgggac gggactgcga cctgagggag atcagcgacg
     6841 ccgcgacgac gggggccacc gggatcgagg gcatcggcgg ctttcagcgg accaacttcg
     6901 cggtcgccga agcggctgcg gcctgtttcc tcggtgaagt ctcccggcgg aagtccgagg
     6961 aggcggtcgg cggcctggtc atccacggcc ggctcgaaat gatcggccag gatccgccgg
     7021 tcttcgccga tgtcgcgcac aatccggccg gggcccgtgc gctggccgcc tcgctgcccg
     7081 aggtgaccgg ggatctgccg gtgatcggcg tgattggcgt gcttgccgac aaggatgccc
     7141 cggggatgct ggccgaactg gccggctcgc ttgaagcggc cgtcttcacc ggtctgcccg
     7201 gggaagccct cgcggcctgg ggcaggcccg gggcacgggc ctgggatccg gaggagcttc
     7261 agggccttgc cgggcggctc gggctcgagg ccgaaacggc caggaccccg gccggggcgc
     7321 tggaaagggc cagggagatg gctttagaga agggcggagc ggtgatcatc accggttcgc
     7381 acttccttct cgcaccgggc gaaggtgccg gcttccaggg ctgaacgacg ggcaaggtct
     7441 ctcagaagcc ggggcggagg gttcggcagc cgccggttat gcgacgatta gccggtgaat
     7501 cgtcagactg gctcccaact gctgtcgatg atggcgctgg tcgctgcggt cgtggcgatc
     7561 gtcattctcg tcttcttcgg cattgggtac ctattcggaa ggatgttcct ctagtcacat
     7621 gcctatcgcc atcttcggaa ttggaaatac cgcgctgaac ctggccgtca ctctggccat
     7681 cctgatgctg gtcgcgatct ggatcgccct catcgtcttc accttcaccg acgcccgtcg
     7741 ccggatcgcc gaccccttcc tggtcggctg cgccaccgcc gcttcgttct tccccttcgt
     7801 cggcacgctc gtctacacga tccttcgtcc gccggagttc ctcgaggaca tccgtgagcg
     7861 cgaaaccgac atcaccgagt ccgaggtgcg gctgcggcac ctcaagcaca gctcctgcca
     7921 caagtgcggc tccccggtcg agagtgactt catccgctgc ccggcctgcc ggtcccggct
     7981 gaaggagccg tgcccgacct gctcgaagcc ggtcggcctg aactggaagg tctgccccta
     8041 ctgcgagacc accctgatcg ctcccaagcg gtcatcgcgc agctccggct cgaagcgccc
     8101 caagggcgaa tcttcgggtc gtccatcatc ttcccgctct ggccgctccg agagggctcc
     8161 cgaagggaag gaagaacgcc gtcgccgctc cagcaaggcc ccggccgagt cgggtgcccg
     8221 cgagagccgg tcttcgtccg gcgaaagccg ccccgaggat tcttcccggc gttcgggcaa
     8281 ctcttcttcc gaccaggccg attcgtcccg ttcgcctcgt tccgcccggc gcaagatcag
     8341 cgtcagcgac gacgagaagt cggaaccgat caaccgcggc gacagctgaa cccctgcgcg
     8401 ggtacgctga cccgccggct cacgccaaac gaaacccgct ggcttcggcc acaaccaaac
     8461 tcacaacgga gtaacaagac atgtcccaga ccctgatcct ggtcaagccc gacgccttcg
     8521 agcgcgccct caccggtgag gttctcgccc gcttcgagcg caagggcctt cgtatcgccg
     8581 ccctcaagaa gctgaccgcg accgagcaga tcgccaacga gcattacgcc gagcacaagg
     8641 agaagccctt cttcggtgaa ctggtcgagt tcatcaccgg tggcccgctg gtcgccgccg
     8701 tcctcgaagg ccccgaagcg gtcgtcgccg cccgccagct gatcggcgcc accaacccgg
     8761 tcgaagccgc gcccggctcg atccgcggcg acttcgccct cgaagtcacc ttcaacatgg
     8821 tccacggctc cgactcggac gagtccgccg cccgcgagat cgcgatctgg ttccccgagg
     8881 gcatctagcc cggagtacta caaccagcct gcagcaccga aagggcggcc ccagggccgt
     8941 cctttcccgt ggctgtccca actcgttgcg aggtgcctat gggagttgtc tatatagaca
     9001 actcccatag gcacctgccc ccgattctgg tctgcaacgg gttctggaag tggctgtgac
     9061 tcccgggtaa ccccgcaaac tttccctttc gatgcccatg ttccaccgca gtgggtcgga
     9121 tttccgtcgc agcagcggga aatccgaccc accggggggt tgcgtgtggg cttggggtcg
     9181 ggtgggcgtt tccgggacac tcagcgagga gcgcggctga atgccgcgcg agggttttgc
     9241 gcgtcccggg aatgcccgcc cgaccccgcg gctcaaccac tgcctcaagg cgggtctagg
     9301 attgctgcgt gggtgtttcc gagaaagttc gtcgtgacga tgcgatccgg ttggcgcggc
     9361 gccactacct caacctcgag cgggtcgaca tgcagtcgct ggccaacgag ttggggattg
     9421 gccgcacgac cctctaccgg tgggtggggg atcgcgactc cctgatttcg gagatgctct
     9481 ccgaaatggt ggtcgaggtg atcggcaccg cgataggcga ggcggagggc gagggccttg
     9541 aacgcacgat cgacgcgatg cgccgattca tgctgacgac cgcggacttc gcgccacttc
     9601 gacacctggt ccagaacgaa cccgaactgg gcctgcgggt gatgctggct ccgggttccg
     9661 gggtttcgac cgcaatcacc ggcgagttga ccgccgagat cgaacggacc cggccggaat
     9721 ggtcgggtga gaaggcaagc gagctggccg acgtcctcac ccagatcggc atggcctacg
     9781 aatggggcaa catcgcggtc cgctccgagc cggagatcga ccgcgcgatc cgggcgatgg
     9841 agaccctggt ccgggccgcc gacacggcct gatcgccggg accgcgatct gcgattctgc
     9901 ttcggtgcca gcctgcccgg taatcctcgc ttccggttca ccccagcgcc gcgacctgct
     9961 cgaacggatc ggcgtcgagt tcgaggttcg ggtctcgggc gtcgaagaga tcgtgcacgg
    10021 cgacccggcg gaggtggtcc tggccaacgc cctgctcaag gccaggtcgg tggccgaaac
    10081 agcgccgggc tgcctggtga tcggttgcga caccgacgtg gtgcttgagg accacatcat
    10141 cggcaaaccg gatgacgagg tccgggcgcg cgaatacctg gcccagcttt ccggccggaa
    10201 acaccgggtc ctgagcgggc tggcgatcgt cggccccgag gcggaacagg tccggaccgg
    10261 ggtggccgaa tcggccgtcc gcttccgtga actggagaag agcgaggttg accgctacct
    10321 ggcgagcggc gagtggcgcg gccgggccgg cggctacgcg atccagggcc ggggatcttc
    10381 cctggtcgcc ggggtcgacg gcgatatcgc caacgtgatc ggcctgccgg ttggcctgct
    10441 actggatttg gctccggaac tggatttatg aaccgctgaa ggcctccggg tagggtggtc
    10501 ggccgctgca atccttcccg cagtcaccag gccgtccggg cggcactctt gccaagctga
    10561 gaaagtcgcg ggttcggagc gattccactc cggatcacga ttggtgcccg ctcttccgcg
    10621 acctctcaat caccaccagc cactcacatg taccgcaaac aagtcagacg tcgccgggcc
    10681 gtgctcatcg gcctcatcgt gctgggcttc gtgctgctca cgttcacctt cggcagtggt
    10741 tcgggcggag tgggaggcgt gctcgggacc ttcttcgacc cggtcaccaa cgccgcctcg
    10801 agggccctga aaccggcccg ggacctgatc aactggttcg acgagacgat ggacgcgcgc
    10861 ggtgagcggg accggctcca gagcgagctc gaggaagccc gcggcaaggc ggtcgccggc
    10921 gaggtcgcac tcgaagacaa ccgccagctc cgcaagatgg tcggcctcgc ggaggccgga
    10981 aagattcccg acgcctacga cccggtgacc gtcaacgtga ccggccgctc gccgaccgtc
    11041 tggtacacga cggtcagcgt caacgccggt tcttccgacg gaatcgcggt caacgatccg
    11101 gtgatcagcg gcgacgggct ggtcgggcgg atcagcgcgg tcagccactc gggttcggtg
    11161 gtcagcctga tctcggacgg gtcgagcgcg gtcagcgcca gggttctgcc cggcggtgcc
    11221 cagggcgtgg tccgggccaa ggtcggggac ccccagactc tggtgctgga atttctcgac
    11281 gaaaccaagg acgtccacag cggtgacacc gtggtcacct ccggctggcg cggcgacggg
    11341 ctctcctcgc tctacccgcg cggcctgccg atcggcgaga tcacgcgggc gccgatcgat
    11401 gagcgcgagg cgatccagtc ggtcgaactg aggcccttcg ccgatctccg caatctggac
    11461 tggctccagg tcctgaccgg aggcaaccgc ggatgatcat cacgagccgc acagccgcga
    11521 gggtggccgg cctgatcctg ctcttcacgg tgcttcagat ctccttcttc tcgcagatcg
    11581 aactgttcgg aacctcgatg tggatcctgc ccgcctgcgc cgcgatcttc ggcctgctgg
    11641 gtggcagcct gatcggggcc acggtcggtt tcgccttcgg cttccttggt gacggactga
    11701 ccgacgggcc gctcggcagc gcctgcctcg tcttcatggc ggtcggctac ctggccggca
    11761 tctaccgcga gcgcggggat ttcgtcgacc ggccgaccgt gctcgcggtc tgcggaatcg
    11821 caaccctggg ggcgaacctg gcgctcggct tctacaccgt gatcgtcggt ttcgattcct
    11881 cgctaagcgg atcgcttgtc cccgacctca tcctgcaggc cttctatggt gttcttctgg
    11941 cgattccgct ctacgcgctg acttaccgtg ttctcagacc agcgctgatt cacgagccgg
    12001 ccacccgccg gcggccggtt gacattgaac ggtgggccga cgagctcgac ccgccgggcg
    12061 gaaacggccc cgaggctttc cgatgagaat tgaacccgta gttcccagac cacgaccgaa
    12121 gcggaacccc gtgtcccgtc ggttcgccgt gatcggcggc atcgggctgg tcctgctggg
    12181 gatcctcttc tttcggctct ggtcgctcca gatcgtgacc ggggacaagt acctcgccga
    12241 ggcaaacgac aaccggaccc gcgagatccg ggtcccggcc ccgcgtgggc ggatcctgga
    12301 ccgggacgga aaggttctgg ttgacaaccg gaccagcatg gcgctccagc tggacccgat
    12361 cgagattccg accaccgtcg cgggccgcca ggagctctac gcgcggatcg gcaaggtgct
    12421 cgatcactct gccgactgga tccggaagaa ggtctacgaa acgaagaaga acgacccgcc
    12481 gggcagcccg atcaccctcg atcaggacgt cgatgacgac atcgtctact acctgcagga
    12541 acaccaggcc gagttccccg aggtccaggt caaccggatt ttcgtccgca agtacccgca
    12601 ggggctgctc gcggcgcaca tcctcggcag catcggcgag gtgaccgccg aggacctcga
    12661 aaagaacccg gacaaggacc tcgccgccgg tgacacgctc
//
LOCUS       DAOQKN010000059        12692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_2315_length_12692_cov_5.879877, whole genome shotgun sequence.
ACCESSION   DAOQKN010000059 DAOQKN010000000
VERSION     DAOQKN010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 12692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12692
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2315_length_12692_cov_5.879877"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..436
                     /locus_tag="PLJ64_05555"
     CDS             35..436
                     /locus_tag="PLJ64_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92984.1"
                     /translation="MRGAQVLEGVRERLEPDGTAAFAISEWIPEGSREVEVLPDVREI
                     GDWVYASRPVEVEDDGDSLTVVRLRQIVGPQGSFEESRDRITLDRIDRKSLADELAEA
                     GLSAVRAIDVPETDRHIATVIVVARHSPRGG"
     gene            453..1187
                     /locus_tag="PLJ64_05560"
     CDS             453..1187
                     /locus_tag="PLJ64_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HNC92985.1"
                     /translation="MKLRLLSLYPDQMNIYADRGNILFLQHRCEWRGIEFEVTRCGPG
                     QRFDPANYDLLYMGGGQDRDQKAVARDLIETKQASMRAAKDGGVAVLAVCGGYQLLGE
                     SYELEGETIAGLGLVDLKTIREPGPRLIGPVSISVNLDGEEKVLAGFENHGGRTRLGQ
                     ASPLGKVIHGHGNNGEDGFEGVREDNLIGTYLHGPLLPKNAWLADHLIATGLEHHHGE
                     RPELEPLEDSLETAAHEAALKAARKG"
     gene            complement(1184..1615)
                     /gene="dut"
                     /locus_tag="PLJ64_05565"
     CDS             complement(1184..1615)
                     /gene="dut"
                     /locus_tag="PLJ64_05565"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HNC92986.1"
                     /translation="MELKVQLLNDSATLPTRAHEGDAGLDLYASEPAHIGPGERWQVA
                     TGIAVEIPEGHAGMVLPRSGLARRHGISLVNAPGLVDAGYRGEVKVLLLNNDPAEVFR
                     INPGDRIAQMVVIPLAAVEPVATDSLSETERGEGGFGSSGR"
     gene            complement(1627..2913)
                     /gene="hflX"
                     /locus_tag="PLJ64_05570"
     CDS             complement(1627..2913)
                     /gene="hflX"
                     /locus_tag="PLJ64_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNC92987.1"
                     /translation="MTESVETSSSRRARQRAMMIAVDTDGQGTLDELGELLRTAGVAT
                     VGEVIQRRSEPDPDRYFGRGKLEEVKAEIKRTGANLVACDDELAPRQERNLEAALEVP
                     VIDRTAIILDIFADHANSAEGKLQVELAQLQYNLARMRGLWTHLERLGAGIGTRGPGE
                     SQIETDRRLARDRISDLRRKLRHVEQNRGVMRSRRQDSSLPQVALAGYTNAGKSTLLN
                     ALTGAGIGVADRLFHTLDPTTRAFEYEGRRYLVTDTVGFIKKLPHQVVEAFKATLEET
                     VLADLILHVVDASAGEDRMQEEMAAADAVLDEIGAGEKPRLLVLNKIDLLDDDQRAEA
                     RIDHPDAVQVSAVSGEGLDLLRARMERAFEETMAPVELLIPYAEGARLHELHEVAGKV
                     DREDRPEGVLVRTRLPLGEMHRFADLATPREAESII"
     gene            2987..3985
                     /locus_tag="PLJ64_05575"
     CDS             2987..3985
                     /locus_tag="PLJ64_05575"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNC92988.1"
                     /translation="MKIFMTGATGFIGGATARHLVEQGHQLTCLVRDRSKAGSLGELG
                     CELVDGDLSSRPRLAEQMKGHDALLHNAALYEVGIPKDRIPALRKANVEGTVNTLEAA
                     LEAGIPRVLYVSTCAVFGNTHGEIATEDFRRPDLDSPGGLEFTSVYEETKYEAHQIAR
                     NLIETRNLPCVIAQPGGVYGPNDHSELGSTIHQFLDGKLPMVPFPEFGTGLTHVEDIA
                     AGLALILDKGEIGESYILNEGNFTMRQILEEVGGIADRKVPKRAMPTVVLKALRPVGP
                     LVGKIMGQPPNLAELIASTDGVTFWADGTKAKTELGWHPREVRTGLRQTLEAEGRL"
     gene            complement(3982..5151)
                     /locus_tag="PLJ64_05580"
     CDS             complement(3982..5151)
                     /locus_tag="PLJ64_05580"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HNC92989.1"
                     /translation="MALTDPSRRLQAMPPYMFAELEKKIADKRAAGIDVISLGIGDPD
                     HPTYPHVVKAMQEAVADRGNQNYPSNRGREEFRRAFSDFYRDRFGVGIDPETEVIPAI
                     GAKECIYNLCFAFLDPGDVALASDPGYPVYTGGPILAGAEAVLLPLKESLGFAPDLEA
                     IDARTAERARLLFLNYPNNPSGAIVPDGFFEQVVDFARENEILVVHDNAYSETTYDGY
                     VAPSFLATPGAKEVGVEVFSLSKGYNMTGWRVGAILGNPDAISTYWRLKTNVDSGLFE
                     AIQLAAVAALDGPSQPLEEMNAIYARRRDLVVDTLRAIGVDVKSPKGTIYVWAPVPKG
                     FTSTSFCEKVLEEAAVVISPGSMYGRSGEGYFRISLTTPDERLSEALERMREHLA"
     gene            complement(5220..6062)
                     /gene="dapF"
                     /locus_tag="PLJ64_05585"
     CDS             complement(5220..6062)
                     /gene="dapF"
                     /locus_tag="PLJ64_05585"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNC92990.1"
                     /translation="MKFEKWQALGNDYVIIEAADLPWEPTQARIQRICDPHFGIGADG
                     VLLISKPQDPKYVADLRIFNPDGSEAELSGNGAREAILYLRHHGWTGEDTFSIMTVAG
                     PVTPTITSDRTCSVEMGRATTTSKDYPDGPADGLGTLASGDRDWKFQHVSIGNPQCAI
                     EAGADLEELDLAKIGPGIENNVLFPNRTNVSFYRVSGSRVRARIFERGVGETLSSGTG
                     ASGAAVAAFLAGAPSPITVELDGGELKVEISDDLEVTLTGWAEPIAAGVLSPEMMSAL
                     ADLG"
     gene            complement(6059..7042)
                     /gene="miaA"
                     /locus_tag="PLJ64_05590"
     CDS             complement(6059..7042)
                     /gene="miaA"
                     /locus_tag="PLJ64_05590"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF013845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNC92991.1"
                     /translation="MAVDPRPGKPPSADPPKAGQPSVLPVIAIFGPTAIGKTGVAIEL
                     AELLRRKGEDPVAINCDSIQVYRGLELISGAASPEDQTRLEHRLLSFVPIDQEFSAGR
                     FGEAARGEIDSALEAGRRPILVGGTGLYLRSALSDLEMRPPVDQELRRQVELEVESRG
                     PAALHAELPPGLSARVHPNDRKRVARLTELSRSGVDPHPDHEGGGELWTSRLRHPTLL
                     VGLVEDDEPLVEKIHRRVGRMAGAGAGAEAERAVANGASRTVRAAIGFDEFQNDDLER
                     VAALHRRYGRRQMTWMRRMEGVQLLDRTGRSDSGVAAEILELAEQVPSRVA"
     gene            complement(7053..8375)
                     /gene="miaB"
                     /locus_tag="PLJ64_05595"
     CDS             complement(7053..8375)
                     /gene="miaB"
                     /locus_tag="PLJ64_05595"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNC92992.1"
                     /translation="MKKFHVTTFGCQMNEHDSERMKGMLTSLGYSESTQRDDADLILF
                     NTCSIRENADNRFISHLGEAKRLKKADPERIVGVGGCWAQSVKDEVFRRFPFVDVAFG
                     PGQIHKLAEFLNSDQLSAQGYFEFEDFAGHLPSHRARQFQGWLQISQGCNCVCSYCIV
                     PSTRGREQSRDPRELLKEAERLAADGVREITLLGQNVNSYGRDLPKESRIRFSDLLGM
                     IDAVDGIDRLRYTSPHPKDMREDVIEAHASLPSLCEHIHLPLQSGSSSILKKMRRTYT
                     RERYMDRVAMIREQVPDCAITTDIIVGFPGETEEDFAETLEVVEEVGYDGAFTFVYSP
                     RRGTLAAEMEDQIAHPVKVERMERLVEAVQRRARERAQRFVGRNMEVLVEGPSRNDPT
                     RIRGRSRHNKAVNFEGTAEAGQMVEVEIGAATSQTLTGTERLLSGIGA"
     gene            complement(8685..8960)
                     /locus_tag="PLJ64_05600"
     CDS             complement(8685..8960)
                     /locus_tag="PLJ64_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92993.1"
                     /translation="MEIKTVFLIFGISLACFAVIITFIGLRSKNFPSKGALIGLLLLA
                     AFLVGGTTTFAVKLSIEEQHEREEGQKGIGEETTDAAAINIIRQDQA"
     gene            complement(9025..10575)
                     /gene="rny"
                     /locus_tag="PLJ64_05605"
     CDS             complement(9025..10575)
                     /gene="rny"
                     /locus_tag="PLJ64_05605"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNC92994.1"
                     /translation="MAFESIIPAWLRNRSPKPEAEGGKETSGPATNGSAPGPASGTGP
                     GAAAPDQPTATSGITPAPDLDSPPPRESLLSAESELRARREEIVRMEERALRDLESAR
                     TRLREADRRSELAEERERRLDRFEQDLEEVREARLAELETVAGMTRDRARQILIREVE
                     EEATRQAGLKVKRIEEEARLDAERRARGILAVAMQRLASTHSIESNTFTIHLPNEEMK
                     GRIIGKDGRNIRALENLTGVDIIIDETPETVVISSFDGVRREVGRLTLEKLIEDGRIH
                     PASCEAAYEEAAAKVDDVMIEAAESALLDARVTGLHPELVRLLGRLRFRTSYSQNVLS
                     HLVECSNLAALMAAELGASVEVARRAALLHDIGKAVSHEVEGTHAAVGARLARRYDEV
                     EAITHAIEAHHNEIEPVTVEAVIVQAADALSGARPGARGDSLEQYVTRLGDLEEIAGR
                     HEGVKKVFAMQAGREIRIMVDPGQVDDEATALISHEIAAAIEREMEYPGQVKITVIRE
                     LRSTRIAR"
     gene            complement(10660..11124)
                     /locus_tag="PLJ64_05610"
     CDS             complement(10660..11124)
                     /locus_tag="PLJ64_05610"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HNC92995.1"
                     /translation="MADELLAKALAAISRKDRTEAEMRAWLAAREAGPEEIDQVIDYL
                     VENLALDDRRFAFGYTSDKRQLAGWGRDRIRENLLRRGIDRYLVEEALNQSAQGGEEV
                     ETEVDRAVRVLGEKGADLSDDRGRQRALGLLARRGYDAEEAYAAIRIAGRAA"
     gene            complement(11153..12289)
                     /gene="recA"
                     /locus_tag="PLJ64_05615"
     CDS             complement(11153..12289)
                     /gene="recA"
                     /locus_tag="PLJ64_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNC92996.1"
                     /translation="MSEKRLNDLQPATEKEAKARVAALEAAQRQIEKDFGAGSLMKLG
                     DQASVNVDAISTGALPLDIALGVGGIPRGRIVEIYGPESSGKTTLVYHIIAEAQKRGG
                     VCAFVDAEHAIDPVYARRIGVNTDELLVSQPDYGEQALEIVDVLTRSGAVDVVAIDSV
                     AALTPRAELEGQMGDVTVGLQARLMSQALRKLAGNLNRANTLCLFTNQIREKIGVSFG
                     SPETQPGGRALKFYSSQRLDIRRIETLKDGTEAVANRVRVKVVKNKVAAPFKQAEFDI
                     EYGLGISREGSLIDLGMEHDLVQKSGSFFSYGETRLGQGRNNSKQFLAENPEIAQEIE
                     DRILANLGISRDGSPIESSAPEGVDAETGEVIETAGGDAELKAA"
BASE COUNT         2065 a         4292 c         4041 g         2294 t
ORIGIN      
        1 cgggcgtcgg gctcgacaac atcgacgcgc aggcgtgcgc ggcgcgcagg ttctcgaggg
       61 cgtccgggag cgccttgaac cggacggcac ggctgccttc gcgatctccg agtggattcc
      121 cgagggctcg cgcgaggtcg aagtgctgcc ggacgtccgc gagatcgggg actgggttta
      181 cgccagccgt ccggtcgagg tcgaggacga tggcgactcg ctgaccgtgg tccgcctgag
      241 gcagatcgtg gggccgcaag gctcgttcga ggaatcccga gaccgcatca ccctggaccg
      301 gatcgaccgg aagtcgctgg ccgatgaact cgccgaagcc ggcctttcgg cggttcgtgc
      361 gatcgacgtg ccggagaccg accgacacat cgcgaccgtg atcgtggtcg cccgtcacag
      421 tccccgaggg ggctgaaccc tagggtttgc gtatgaagct tcgcctgctc agcctctacc
      481 cggaccagat gaacatctac gccgaccggg gcaacatcct ctttctgcag caccgctgcg
      541 agtggcgcgg gatcgagttc gaagttaccc gctgcgggcc gggccagcgc ttcgacccgg
      601 ccaattacga cctgctctac atgggtggag gccaggaccg ggaccagaag gcggtcgcca
      661 gggacctgat cgaaaccaaa caggcttcga tgcgcgcggc caaggatggc ggcgtggccg
      721 tgctcgccgt ctgtggcggc tatcagctcc tgggcgagtc ctatgagctg gaaggcgaaa
      781 cgatcgccgg cctcggcctg gttgacctga aaaccatccg cgaaccgggc ccaaggctga
      841 tcggaccggt ttcgatcagc gtcaaccttg acggtgaaga gaaagtcctg gccgggttcg
      901 agaaccacgg cggaaggacc cgcctcggcc aggcgtcacc gctcgggaag gtcatccacg
      961 gccacgggaa caacggagag gacggtttcg aaggggtccg cgaggacaac ctgatcggca
     1021 cctacctgca cggccccctg ctcccgaaga acgcctggct ggccgatcac ctgatcgcga
     1081 ccgggctcga gcatcaccac ggcgagcggc ccgaactcga gccgctcgaa gattcactcg
     1141 aaacagccgc ccacgaagcg gcgctcaagg cagcccgcaa gggctagcgg ccgctcgagc
     1201 cgaagccgcc ctcgccccgt tcggtctccg acagcgagtc ggtcgcgacc ggttccaccg
     1261 cggcaagcgg gatcacgacc atctgagcga tgcggtcgcc tgggttgatc cggaatacct
     1321 cggccggatc attgttcagc agcagcacct tcacctcacc gcggtagccg gcgtcgacca
     1381 ggcccggcgc gttgaccagg gaaatgccgt gccgacgggc cagacccgaa cggggaagga
     1441 ccatcccggc gtgaccttcc ggaatctcga ccgcgatccc ggtcgccacc tgccagcgtt
     1501 cacccgggcc gatgtgagcc ggctccgagg cgtagaggtc cagcccggcg tccccttcgt
     1561 gggcgcgggt cggcagggtc gcggaatcgt ttagcaactg aaccttgagt tccatcgccg
     1621 gggactctag atgattgatt cggcttctct tggtgtcgcg aggtcggcga agcggtgcat
     1681 ctcgccgagc ggcagtcggg ttctgaccag caccccttcc ggccggtcct cgcggtcgac
     1741 cttgccggcc acttcgtgaa gctcatgaag acgagcgcct tcggcgtatg ggatcaacag
     1801 ctcgaccgga gccatcgtct cttcgaaggc acgctccatc cgggcccgca gcaggtcgag
     1861 gccttccccg ctgaccgccg aaacctggac cgcatcggga tggtcgatcc gcgcctccgc
     1921 ccgctggtca tcgtccagca ggtcgatttt gttcaggacc agcagacggg gcttttcacc
     1981 ggccccgatc tcatcgagca cggcgtcggc ggcagccatt tcctcctgca tccggtcctc
     2041 gcccgccgag gcgtcgacca catgcaggat caggtcggcc agcacggttt cctcgagggt
     2101 tgccttgaag gcttcgacca cctgatgggg cagcttcttg atgaacccca ccgtgtcggt
     2161 gaccaggtac cgccggcctt cgtactcgaa ggcgcgcgtg gtcgggtcga gcgtgtggaa
     2221 cagcctgtcg gccaccccga taccggctcc ggtcagggcg ttcagcaggg ttgacttgcc
     2281 ggcgttggtg tagccggcca atgcgacctg cggcagcgag gaatcctgcc ggcgacttcg
     2341 catcacaccg cgattctgtt cgacgtgccg gagcttccgg cgcaggtcgg agatccggtc
     2401 gcgggccagc cggcggtcgg tctcgatctg ggattcgccc ggccccctgg tgccgattcc
     2461 cgcgccgagc cgctcgaggt gggtccagag gccccgcatc cgggccaggt tgtactgcag
     2521 ctgcgccagc tccacctgca gcttgccttc ggccgaattg gcgtggtcgg cgaagatgtc
     2581 gaggatgatc gcggtacggt cgatcaccgg cacctcgagg gcagcctcaa ggttccgttc
     2641 ctgccgggga gccagttcgt cgtcacaggc gacgaggtta gcgccggtgc gcttgatttc
     2701 cgccttgacc tcttccagct tgccgcgacc gaagtaacgg tccggatccg gttcgctgcg
     2761 gcgctggatc acctcgccaa ccgtggcgac gccggcggtc cggagcagct cgccgagttc
     2821 gtccagggtc ccctgcccgt ccgtatcgac ggcgatcatc atcgcccgct gccgggccct
     2881 gcgactgctt gaggtttcaa ctgactcggt cactgacttc atcctagaac tcgcaggacg
     2941 gcggccaact ccgggccgcc cgcgggctcg cgctacgctc ccctccatga agatcttcat
     3001 gacgggagcg accggtttca tcggcggtgc gacggccagg cacctggtcg agcagggcca
     3061 tcagctgacc tgcctggtcc gggacaggtc gaaggcaggc agcctgggtg aactcggttg
     3121 cgagctggtt gacggtgacc tttcgtcgcg gccccggctg gccgagcaga tgaagggcca
     3181 tgacgccctg ctccacaacg ccgcgctcta tgaggtcggc atccccaagg atcgcatccc
     3241 ggcacttcgc aaggcaaacg tcgaaggcac cgtcaacacc ctggaagcgg ccctcgaagc
     3301 cggcatcccc cgggtcctgt acgtctcgac ctgcgccgtc ttcggcaaca cgcacggcga
     3361 aatcgcgacc gaggacttcc gccggcccga tctcgacagt cccggcggcc tcgagttcac
     3421 ctcggtttac gaggaaacca agtacgaggc tcaccagatc gccaggaacc tgatcgagac
     3481 ccggaacctg ccctgcgtga tcgcccagcc cggcggggtc tacgggccga atgaccactc
     3541 ggagctcggc tcgacgatcc atcagttcct cgacggcaag ctgccgatgg ttcccttccc
     3601 cgagttcggc accggcctga cccatgtcga ggacatcgcg gccggcctgg ccctgatcct
     3661 cgacaaggga gagatcggcg agagttacat cctcaacgaa ggcaacttca cgatgcgcca
     3721 gatcctcgag gaggttggcg ggatcgccga ccgcaaggtt cccaagcggg cgatgccgac
     3781 cgtcgttctc aaggctttga gaccggtcgg acccctggtc ggcaagatca tgggccagcc
     3841 gcccaatctg gccgagctga tcgcctcaac cgacggcgtc accttctggg ccgacgggac
     3901 caaggccaag accgaactgg gctggcaccc gagagaggtc agaaccggcc tccgccagac
     3961 cctcgaagcc gaaggaagac tctaggcgag gtgctcgcgc atccgctcga gggcctcgct
     4021 gaggcgctcg tcgggtgtgg tcagcgagat gcggaagtac ccttcgccgc ttcgcccgta
     4081 catcgaaccg ggtgagatca cgaccgctgc ctcttccaga accttctcgc agaagctggt
     4141 cgaggtgaag cccttcggca ccggggccca gacgtagatc gtgcccttgg ggctcttcac
     4201 gtccacgccg atcgccctca gggtgtcaac caccaggtcg cggcgtcgcg cgtagatcgc
     4261 gttcatctct tccagcggct gggaagggcc gtcgagggcc gccaccgcgg ccagctggat
     4321 cgcctcgaaa agaccggaat cgacgttggt cttgagccgc cagtaggtcg agatcgcatc
     4381 cgggttgccg aggatcgccc cgacccgcca gccggtcatg ttgtagccct tcgaaagcga
     4441 gaagacctcg actccgactt ccttcgcccc gggggtcgcg aggaagctcg gggcgacata
     4501 tccgtcgtag gtcgtctccg agtaggcgtt gtcgtgaacc acgaggatct cgttctcgcg
     4561 ggcgaagtcg acgacctgtt cgaagaagcc gtcgggaacg atcgcgccag aggggttgtt
     4621 cggatagttg aggaagagca gccgggcccg ctcggcggtc cgggcgtcga tcgcttcgag
     4681 atcaggggca aaacccaggc tttccttgag cggcagcagc accgcctcgg cgccggcgag
     4741 gatcggaccc cccgtgtaga ccgggtatcc cgggtcggaa gcgagggcaa cgtcacccgg
     4801 gtcaaggaag gcgaagcaga ggttgtagat gcattccttc gccccgatcg ccgggatcac
     4861 ttcggtttcc gggtcgatcc cgaccccgaa acggtcccgg tagaaatcag agaaggcccg
     4921 ccggaactcc tcccggccgc ggttgctcgg gtagttctgg ttgccccggt ctgcaactgc
     4981 ctcctgcatc gccttgacca catgcgggta ggtcggatga tcgggatcac cgatcccgag
     5041 gctgatcacg tcgattcccg cggcacgctt gtcggcgatt ttcttttcga gctcggcgaa
     5101 catgtacggg ggcatcgcct ggagtcgtcg ggatggatca gtcagcgcca cggtctctcc
     5161 ttgtcggatc ggtttgaatt cgaagtctgg cgggctcgcc cggctgcggg cgggcgggat
     5221 cagccgaggt cggccaatgc gctcatcatt tcaggcgaga gcacaccggc tgcgatcggc
     5281 tccgcccagc cggtcagcgt cacctcgaga tcatcgctga tctctacctt gagttcgccg
     5341 ccgtcgagtt cgaccgtgat cgggctgggg gcgccggcga ggaaggccgc gaccgccgca
     5401 ccggaagctc cggttcccga agaaagagtt tcacccacgc cccgttcgaa aatccgggcg
     5461 cgcacccggg aaccggagac ccgatagaac gagacgttgg tccggttcgg aaagagcacg
     5521 ttgttttcga ttcccgggcc gatcttcgcc aggtcgagtt cctcgaggtc ggccccggct
     5581 tcgatcgcgc attgcgggtt gccgatcgag acgtgctgga acttccagtc ccggtcgccc
     5641 gaagcgagcg tgccaagccc gtccgccggt ccgtccggat aatccttcga agtggtcgtc
     5701 gcccggccca tttcaaccga gcaggtgcga tcggaagtga tcgtcggggt gaccggaccg
     5761 gcgacggtca tgatgctgaa agtgtcctcg ccggtccagc cgtggtgtcg caggtagagg
     5821 atcgcttcac gggctccgtt tcccgaaagc tcggcctcgg acccgtccgg gttgaagatc
     5881 ctcagatcgg cgacgtactt cggatcctgc ggtttcgaga tcagcagcac cccgtcggct
     5941 ccgatcccga aatggggatc gcagattctc tggatccggg cctgggtcgg ctcccagggc
     6001 agatcggcgg cctcgatgat cacgtagtcg ttgccgagcg cttgccactt ttcgaacttc
     6061 atgcgacccg ggaaggtacc tgttcggcaa gttcgaggat ctctgccgcc accccggaat
     6121 cgcttctgcc ggtccggtcg agcagctgaa ccccctccat tcggcgcatc catgtcatct
     6181 ggcgccgtcc gtacctgcgg tggagtgcgg ccacccgctc gagatcatcg ttctggaact
     6241 cgtcgaagcc gatcgccgcc cggaccgtcc gggatgcccc gttggcgacc gcccgttcgg
     6301 cctcggcacc ggctcctgcc ccggccatcc ggccaaccct ccgatggatc ttctcgacca
     6361 gcggctcgtc gtcttcgacc aggccgacca gcagggtcgg gtgtctaagc cggctcgtcc
     6421 acagttcgcc gcctccctcg tgatccgggt gggggtcgac gccggaacgg ctcagttcgg
     6481 tcagccgcgc cacccgcttg cggtcgttgg gatggacccg ggccgaaagc ccgggcggga
     6541 gttcggcatg gagcgctgcc ggcccccggg actcgacttc gagttcgacc tgccggcgaa
     6601 gttcctgatc gaccgggggc cgcatctcca ggtcggatag ggccgagcgc agatagagcc
     6661 cggttccccc gaccaggatc gggcggcgcc ccgcctccag ggccgagtcg atctcgcccc
     6721 gggcggcttc gccgaaccgc ccggcgctga actcctgatc gatcggaacg aaagagagca
     6781 gccggtgctc gagcctggtc tggtcttccg gcgaggcggc gccgctgatc aactccaggc
     6841 cccggtaaac ctgaatcgag tcgcagttga tcgcgaccgg gtcctccccc tttcgccgaa
     6901 gcagttcggc aagttcgatc gccactccgg tcttcccgat cgcggtcggg ccgaagatcg
     6961 cgatcaccgg caggaccgat ggctgcccgg ccttcggcgg atctgccgaa ggcggctttc
     7021 ccggtcgcgg atcaaccgcc atccggctct cctcaggcgc cgatgccgct gagcaggcgc
     7081 tcggtgccgg tcagggtctg tgaggtggcg gcgccgatct ccacttcgac catctggccc
     7141 gcttcggcgg tgccttcgaa gttgaccgcc ttgttgtgac ggctgcggcc acggatccgg
     7201 gtcggatcgt tgcggctcgg gccctcgacc agcacttcca tgttgcggcc gacgaacctc
     7261 tgcgcccgct ccctggcccg gcgctggacc gcctcgacca gccgttccat ccgttcgacc
     7321 ttgaccggat gggcaatctg gtcttccatc tcggcggcca gggtgccgcg acggggcgag
     7381 tacacgaagg tgaaggcgcc gtcgtacccg acctcctcca ccacttcaag ggtctccgcg
     7441 aagtcttcct cggtttcccc ggggaacccg acgatgatgt cggtggtgat cgcgcagtcc
     7501 ggaacctgct cccggatcat cgcgacgcgg tccatgtacc gctcgcgcgt gtaggtgcga
     7561 cgcatcttct tcaggatcga actggacccc gactgaagcg gcaggtggat gtgctcacac
     7621 agcgagggca ggctcgcgtg agcctcgatc acgtcttcgc gcatgtcctt tggatgcgga
     7681 ctggtgtacc tgagccggtc aatcccatcg accgcgtcga tcatgccgag caggtcggaa
     7741 aaccggatcc ggctttcctt cggcaggtcg cggccgtagg agttgacgtt ctggcccagc
     7801 agggtgattt cccgcacgcc atcagccgcc agccgttcgg cttccttgag cagctcccgg
     7861 gggtcgcggc tctgttcccg ccccctggtc gaaggcacga tgcagtacga gcagacgcag
     7921 ttgcagccct gggagatctg cagccagccc tgaaactgcc gggcccggtg cgagggcagg
     7981 tgaccggcga aatcctcgaa ttcgaagtag ccctgggcgc tcagctgatc cgagttgagg
     8041 aattcggcca gcttgtggat ctggccgggg ccgaaagcca cgtcgacgaa ggggaatcgc
     8101 cggaagacct catccttgac cgactgggcc cagcagccac caactccgac gatccgttcc
     8161 ggatccgcct tcttcagccg cttcgcctcg ccgaggtggg aaatgaaacg gttgtccgcg
     8221 ttctcgcgga tcgagcaggt gttgaacagg atcagatccg cgtcatcgcg ctgggtcgat
     8281 tccgagtagc caagcgatgt gagcatgccc ttcatccgtt cggaatcgtg ctcgttcatc
     8341 tggcacccga aggtggtgac gtggaacttc ttcatgggca gttcagatta cagtcggccg
     8401 tcaagccacg cccaaccgac cccacgacta catcaacccg gattcaaggc acggtgccgc
     8461 aggttcgcga cctctccggt accaaacctg cgtcaccccc tgatctgaag gcgcggtcag
     8521 cgggggttaa tcagccgggg accggtgacc ctcccaaagt gccttcgcga ttactgctcc
     8581 gaccatcgag caggcacgcg ttcacgcgac ggtagaggca tctttgggag ggtctccggc
     8641 ccccggcgct gcgcgtcccg ggaagccgag ccggtgcgga aggcttaggc ctggtcctgg
     8701 cggatgatgt tgatggcggc ggcgtcggtg gtttcctcgc cgatgccctt ctggccctct
     8761 tcacgctcat gctgttcttc gatcgagagc ttgaccgcga aggtggtggt gccaccgacg
     8821 aggaacgcag ccagcagcag aagcccgatc agggcgccct tgctggggaa gttcttcgag
     8881 cgcagcccga tgaaggtgat gatcaccgcg aagcaggcga ggctgatgcc gaagatgagg
     8941 aagacggtct tgatttccat cggcgcgaat cctactgatt tcgacggcgt tccggcggca
     9001 tcccgatcag cggtccggag ctccctaccg ggcgatcctg gtcgatcgga gttccctgat
     9061 caccgtgatc ttgacctggc ccggatactc catttccctt tcgattgccg ccgctatttc
     9121 atgcgaaatc agcgccgtcg cctcgtcatc gacctgtccg ggatcaacca tgatcctgat
     9181 ctcccggccc gcctgcatgg cgaagacctt tttcaccccc tcgtggcgac cggcgatctc
     9241 ttccaggtca ccgagccggg tcacgtattg ctcaagggaa tcccccctgg cgccggggcg
     9301 ggcacccgag agagcgtcgg ccgcctgaac gatcacggct tcgaccgtga ccggctcgat
     9361 ttcgttgtgg tgggcttcga tcgcgtgagt gatcgcttcc acctcgtcgt accggcgggc
     9421 caggcgcgcc cccaccgctg cgtgggttcc ctcgacttca tggctcactg ccttgccgat
     9481 gtcgtgaagc agggccgccc gacgggcaac ctcaaccgac gcccccagtt cggccgccat
     9541 cagcgcggcc aggtttgaac actcgaccag gtggctgagc acgttctggc tgtaactggt
     9601 ccggaaccgg aggcgaccga gcagtcgcac gagttctgga tggaggccgg tgacccgggc
     9661 gtccagcagg gccgattccg ccgcttcgat catcacgtca tcgaccttgg cggcagcctc
     9721 ctcgtaggcg gcttcacagg aagcgggatg gatgcggccg tcctcgatca gcttctccag
     9781 ggtcagccgg ccaacctcgc gacgcacccc gtcgaagctc gagatcacca ccgtctccgg
     9841 agtttcgtcg atgatgatgt cgactccggt caggttctcc agcgcccgga tgtttcgtcc
     9901 gtccttgccg atgatccggc ccttcatctc ctcgttcggc aaatggatcg tgaaggtgtt
     9961 cgactcgatc gagtgggtgg aggcgagccg ctgcatcgcc accgcgagga tgccgcgggc
    10021 ccggcgctcg gcatcgagac gggcttcctc ttcgatccgt ttcaccttca gaccggcctg
    10081 gcgggtggct tcttcttcaa cctccctgat caggatctgc cttgcccggt cgcgggtcat
    10141 gccggcgacc gtctcgagtt cggccagccg ggcttcgcgg acttcttcga ggtcctgctc
    10201 gaaccggtcc agcctgcgct cgcgctcctc ggcgagttcc gaccgccggt ctgcctcgcg
    10261 caggcgggtc cgggcagatt cgagatcacg cagagcacgc tcttccatcc ggacgatttc
    10321 ttcccgccgg gcccgcagtt ccgactcggc cgaaagcagg ctttcccttg gcggtggcga
    10381 gtccaggtcg ggggccggcg tgatgcctga tgtcgccgtt ggctgatccg gagccgcagc
    10441 tcccggaccg gttccggatg ccggccctgg cgccgagccg ttggttgccg gcccgctggt
    10501 ctctttcccg ccttcagcct ccggtttcgg agaccggttc cgcagccagg ccgggatgat
    10561 cgactcgaac gccatgcctc cctcaagggt agccgacgct tcaactcccc ttttcgacag
    10621 cccccgcagg caagattcag taccggtccg gtgctccgct caggccgcgc gaccggcgat
    10681 gcggatcgcg gcgtaggcct cctcggcgtc atatccgcgc cgcgcgagca gcccgagggc
    10741 acgctgcctt ccccggtcgt cggagaggtc agcgcccttc tcgccgagca cccgcactgc
    10801 cctgtccact tcggtttcga cctcttcccc gccctgagcg gactggttca aagcctcctc
    10861 gaccaggtaa cggtcgatgc cccgccggag caggttctcc cggatccggt cacggcccca
    10921 gccggcaagc tgtcgcttgt cactggtgta tccgaaagca aaccggcggt cgtcaagcgc
    10981 aaggttctcg accagatagt cgatcacctg gtcgatctct tcgggcccgg cttcgcgcgc
    11041 ggcgagccag gcccgcattt ccgcctcggt ccggtccttg cggcttatcg ccgcgagggc
    11101 tttcgcaagc agctcgtcag ccatcaaccg gacctggccg ggctgaccgg cctcaggccg
    11161 ccttcagttc ggcatcgccc ccggcagtct cgatcacctc accggtctcg gcatccacgc
    11221 cttccggcgc cgaactctcg atcggcgagc cgtcccgcga aatcccgagg ttggcgagga
    11281 tccggtcctc gatctcctgc gcgatctccg ggttctcggc cagaaactgc ttcgagttgt
    11341 tgcgaccctg cccgagccgg gtttctccgt aggagaagaa ggaaccggac ttctggacca
    11401 ggtcatgttc catgccgagg tcgatcaggc tgccctcgcg ggaaattccg aggccgtatt
    11461 cgatgtcgaa ctccgcctgc ttgaacggcg ccgccacctt gttcttgacc accttcacgc
    11521 gaacccggtt ggcgaccgct tcggtcccgt ccttgagggt ctcgatccgg cggatgtcaa
    11581 gccgctgcga ggagtagaac ttgagcgccc ggccgcccgg ctgggtttcc ggggagccga
    11641 aggacacgcc gatcttctcg cggatctggt tggtgaacag gcaaagggtg ttggcccggt
    11701 tcaggttgcc ggccagcttc ctcagtgcct gggacatcag cctggcctgc aggccgaccg
    11761 tcacgtcgcc catctggcct tcaagctcgg cccgcggggt gagtgccgcg accgagtcga
    11821 tggccaccac gtccaccgca ccggaccggg tcagcacgtc gacgatctcc agcgcctgct
    11881 cgccgtagtc cggctgcgag accagcaact cgtcggtgtt cacgccgatc ctccgggcgt
    11941 agaccgggtc gatcgcgtgt tcggcatcga cgaaggcaca gactccgccg cgcttctggg
    12001 cctcggcgat gatgtggtag acgagggtcg tcttgcccga ggattccggc ccgtaaatct
    12061 cgacgatccg gcctcgcgga atgccaccca cgccgagcgc gatgtcgagc ggcagcgcgc
    12121 cggtcgaaat cgcgtccacg ttgaccgaag cctggtcgcc cagcttcatc agcgacccgg
    12181 caccaaagtc cttctcgatc tgccgctggg cagcctcgag ggcagcaact ctggccttgg
    12241 cctccttctc ggtggccggc tgcaggtcat tcagtctctt ttcgctcatc tcattcacct
    12301 cgccgccgcc ctgacgggca gatcgttgat cctctcgtgc ccgaacggac acgaacacaa
    12361 gttcgtttcg ggaacagtaa gtcctgcttc ggacggaact tttggaacac agctcctgga
    12421 ctggaatctg cccgaaaaat cctcaaaagt gtgcaaacca cgcaacaatt ccaacacaaa
    12481 agcgtgccaa aacagtgaca accacagtcg gacggcatcc gagtcaccac ctgcggagcc
    12541 agcccaacca tcattggctt ctgtgggctg gcgggtgtga cttggagggc gggtccgacc
    12601 cgcctgccaa caaagccagc ccatccattt atggatgtcc tgggctggcg tgtgaaattt
    12661 ggagggcggg tcggaccccg cccgactcct gt
//
LOCUS       DAOQKN010000060        12566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_2344_length_12566_cov_6.287027, whole genome shotgun sequence.
ACCESSION   DAOQKN010000060 DAOQKN010000000
VERSION     DAOQKN010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 12566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12566
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2344_length_12566_cov_6.287027"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="PLJ64_05620"
     CDS             complement(<1..275)
                     /locus_tag="PLJ64_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC92997.1"
                     /translation="MGLLEGKNAIVTGSARGIGRATAELFAAHGAKVLINDLDADIAE
                     QTASEIDGETAVFGGDLTKGDACDQLVATAQDAFGSIDIVVNNAGYT"
     gene            452..1909
                     /locus_tag="PLJ64_05625"
     CDS             452..1909
                     /locus_tag="PLJ64_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92998.1"
                     /translation="MKTNRRISKACTGANFVLTLVFVVALFSWFTQVPDAQAANVSFV
                     GDDGNVYRTSPDGTITEQVTTDATAETRYVTPTQKNDGTIVAIKRVSSNAFAYFLNPE
                     NGQVKDTWLLPKTGAGSFAPFNGGAISPDGGMFVYDWHYFDCWTNPCTLNQKVSFISG
                     PGTTNPCLVNCHTYYIRPRWIPGTPYAGFVDTDFRRIWVQKAGSAEPNAWLGFNDPNA
                     GDMHSFDVSSNGLTVLEVSPEGSEAAEFSFWNTGGTPPAGTPSHRCSITAIAQTPAYP
                     RFSPDGSMITWQDRGSIYVAPVPSTTGGGSCNLNPVKVGEGMEPSFGLATLKRDEPTD
                     PTDPTDPTDPTDPTDPTDPTDPTDPTDPSDPSDPSDPTDPGPGTPKVTLKVNPGTAKV
                     KAGRPVTLTAAVTASGANAPTVKVCATVSGKARNVIKPAACRTLRNVARGSTVRTSLV
                     IRTAKKARGSYPVRVAASGPGLTPVNSNNKVQVTK"
     gene            1906..2448
                     /locus_tag="PLJ64_05630"
     CDS             1906..2448
                     /locus_tag="PLJ64_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC92999.1"
                     /translation="MNTRNHSTPEMINRPPESRPGETRLLDGGFRAGLALFTCLFAFW
                     FMFAADDAGAAAYKGKTKGGSSITFALKGNRVTGIRTVVPTLCLETTGGYTSRAGSEL
                     FQPVAAGRIGRTVKSKALQPAAMNVGAKATKNYEVTVNRRGRTVSGKLKLNFSFLIPD
                     LYGARIYICSGVTTFSATPR"
     gene            2507..2899
                     /gene="mscL"
                     /locus_tag="PLJ64_05635"
     CDS             2507..2899
                     /gene="mscL"
                     /locus_tag="PLJ64_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HNC93000.1"
                     /translation="MIQGYREFITRGNVIDLAVAVVIGAAFTALVNSVVADLLTPIIA
                     AIIGEPDFSALSFTIRGSEFTYGNFINALIAFLSITAVIYFFVVLPLNHLKEAEEETT
                     RECPECLSEIPNKATRCAHCAAEVTPTA"
     gene            complement(2902..3423)
                     /locus_tag="PLJ64_05640"
     CDS             complement(2902..3423)
                     /locus_tag="PLJ64_05640"
                     /inference="COORDINATES: protein motif:HMM:NF012417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNC93001.1"
                     /translation="MKVGHRIRWSHPTGRRIRIGLAALALVAVGGAGTAIAISATSDP
                     PAPKYNSLARVVNPIGGKGRALNLARVVIQPGAKIPLHYHQGTQVGYIASGVLTYHVQ
                     SGQAKLMSGAGDAPKLVRLIKAGQTAKVKPGQWLIEQPSDHHRAANNGTKPVVILLSN
                     LLLKDAAPSRLIP"
     gene            complement(3518..4987)
                     /locus_tag="PLJ64_05645"
     CDS             complement(3518..4987)
                     /locus_tag="PLJ64_05645"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNC93002.1"
                     /translation="MTEPVEEVDVVIVGAGISGISMAHQIGLDHPGLSYAVLENRTRI
                     GGTWDLFRYPGIRSDSGMGTFAFSFRPWPGDRHFGNGDQIQQYLEDTAAEYGIDRHIR
                     FGRKVVGADWDSAEARWTVTVAHSAENGSSRGSTSRIRCRFLFGCTGYYRYDKGFSPV
                     FEGEEDFEGELFHAQHWPEGLDPRGRRFVVIGSGATAITVVPALVEAGADVTLLQRSP
                     TYVLSIPTTGGIGGWIRSRFSPRRAAFLLRWFGILMEIVNFNLARRYPNFFRRLFISG
                     ARKQLPPDIERRHLNPRYDPWTQRVCFAPDGDYFAALSGDSARIETGNIERISSDRIH
                     LREGGEIEADVIVKATGLELLLFGDIAMTVDGEPVNPAERLVYKGAMLSGIPNFVFVV
                     GYTNASWTLKSELIARFASRFIGEVDRAGAASGTPVAPEIPMKTVPLLNLDSGYILRA
                     RDRLPLAGDTSPWVLHMNYFSDRRELLGADMHDGALRYS"
     gene            complement(5027..6229)
                     /locus_tag="PLJ64_05650"
     CDS             complement(5027..6229)
                     /locus_tag="PLJ64_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNC93003.1"
                     /translation="MSTETVIKPDHEAGRKHFIFTQEHEDLRESMTDWVKKELHPHRM
                     EWEETKWPDSAMKRAGELGYLGLCFPEEYGGQGGDYYYSLVRAEALSYSGSGGLGMGF
                     AVQTDMVLPPIHLLGTEEQKQKYLAPGITGEKIGALGITEPGAGSDVAGIRTTAILDG
                     DEYVINGSKTFITNAARADFMVLVTKTDPDAGHDGITLMLIDIRDEDGNDLPGFSVAQ
                     NLEKMGMHASDTGELAFEDFRVPADSVLGQVGKGFYHISWELQSERLVAAAGSNASAE
                     RLLETAIEYAGERNAFGRPIGKFQAIRHKIADMATKIEASKQFTYSVAWRVNNGEYPV
                     REISMAKLLSAQMACEVADQTIQVLGGYGYMKEYEVERAYRDIRLNRIGAGTDEVMLE
                     VIGRSYGL"
     gene            6305..7102
                     /locus_tag="PLJ64_05655"
     CDS             6305..7102
                     /locus_tag="PLJ64_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNC93004.1"
                     /translation="MSQATPDFGKIRYEVADHVATITLDDPDTRNALSDQLLGELISA
                     LHLAREDTEVRVVVLASSHETTFSAGGNLAAFGGDANAVQKFEGAELFVESFKLLGQL
                     GKPSIVAARGHVLAGSLGLALACDLIVASEEATFGTPEINVGLFPFMIMALIYRNVPR
                     KKANEMLLLGGRMEAKDAKEAGIVNFVAKKKDFDLVLEDWTTMLARKSPLIMKLGKDA
                     MWRQMDMDLAAALDYLRSQLAIELSSEDAIEGVKAFFEKREPEWKGR"
     gene            7102..8706
                     /locus_tag="PLJ64_05660"
     CDS             7102..8706
                     /locus_tag="PLJ64_05660"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNC93005.1"
                     /translation="MSENENGTEAGQNLLPGTAPLTALTKQLHERRGKARLGGGLEKI
                     EKQHAAGKLTARERIDLLVDEGSFNEIGIHGRPHYSQPAMQSKEAPADGVVTGWGDVD
                     GRPVAIVAYDFTVMAGSMGQTGEIKVTRIREMALQKRMPLVWLLDSAGARIQEAAGSL
                     FAGSGHLFREEVQMSGVVPMVAAMMGPCAAGTAYIPGLSDFVPMVVGQGAMALAGPHL
                     VKAVTGEEIEMEDLGGAKVHCRKSGVGDLEVKDDPECIESIKKYLSYFPSNCEQKPPV
                     TETADPEDRGSEKLLEIIPDSPRTPYNMYEVIEEIVDDGDWFDIKPKFAKSLITCLAR
                     MGGQPVGIVANQPKVLGGVLDVDSADKAARFINLCDAFNIPLLFLQDVPGFLVGSKVE
                     QQGIIRHGAKMLYAVSRATVPKITVLVRKAYGAGYYVMCGRAYEPDLIVAWPGAEISV
                     MGAEGAVNIIGRSAIEAADDPEATREAMLNAVRQQIDPYIAAGNAMIDDIIDPRETRQ
                     TVIRGLRMAKDKHVERPWKKHGVMPV"
     gene            8703..9038
                     /locus_tag="PLJ64_05665"
     CDS             8703..9038
                     /locus_tag="PLJ64_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93006.1"
                     /translation="MSEVIEVTIPHEQVQLNGVIRFDGSVVEIFGFNDVHSVRMHIQQ
                     IEFAEVKFDKGLLAQPRIMFKGKWSNLGYNQAIDPPDQIKPELENFVNAINAAIGNDA
                     ANYPNGETQ"
     gene            9035..9913
                     /locus_tag="PLJ64_05670"
     CDS             9035..9913
                     /locus_tag="PLJ64_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HNC93007.1"
                     /translation="MSKFEDPVIISNSISGVIANRDQCPAIPYTPEEYAAEARRSVDE
                     GASQIHIHARTPDGTPSYEIEDFQAITEAIKAECPEVIINYSTGAIGVPIEKRIAYLE
                     ACRPDVAALNMSSMNYAKYSKRRKDFVFKAVFENSFDTIVEFITAMNDLGIKPEHECF
                     DAGHVANLDPLYDMELIKPPSQISLVMGVTGGIRPTPRNVAVMADQVPGGPEGPNNWQ
                     VIGISRDQWKLLAAAITLGGNVRAGVEDNLYLPDGTMCKSNGELIGKARQMVEDAGRK
                     VATIAEAREMLGLKAA"
     gene            9910..11142
                     /locus_tag="PLJ64_05675"
     CDS             9910..11142
                     /locus_tag="PLJ64_05675"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HNC93008.1"
                     /translation="MSLPLEGIRVLDLTRLLPGGFCSQMLVDFGAEVIKVEDTGPGDY
                     IRYAPPLYESDADPAEATASGLYLSLNRGKRSIRLDLKSEAGREAFYRLLPTADVVLE
                     GFRPGVAAKLGVDYETLSRKNEGLVYCSISGYGQDGPSRDRAGHDINYLGSTGMLAMT
                     GQAGGPPVQPAGQIGDLGGGAMNAAFGILAALIEKGRSGKGQMVDISMTDGAFAWLAM
                     AAGAYFAGGEVPRRGEVMLGGGIVCYLTYEAADGWVSCGALEPKFWCAFCEGTGHEDL
                     IEIQFERPGGEAWEKVAAVFRERTRDEWKSFNDQYDCCIEPILELDEAVESDLLRERG
                     MIVEWEQPGIGPVRQIGSPIKMSRTPAAEPGPAPAIGADTREVLASAGLSAEEIEGLF
                     ESGVAAGPGSGGEMEFRA"
     gene            11139..11852
                     /locus_tag="PLJ64_05680"
     CDS             11139..11852
                     /locus_tag="PLJ64_05680"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNC93009.1"
                     /translation="MTQAAAEEKPDAPAEQLLKMSGLVEASGVPAPTIKHYLREGLLP
                     EPVRTSRNMAWYRPETVERIRLIKRLQEERYLPLKAIRALFEEDPAQAEAMLEVEDRI
                     LERALAGERTRTSATEVRKYGVPKKVLERLAEIGILTPNSRGYSPSDVEIIEAMSRFR
                     AGGYDEQIGFTVYDTLRYKHALEALVKEEVDVLMERLEGEITPEHAVELIEAGAEPLK
                     DLIAALHTKLMVEEVERRR"
     gene            11889..>12566
                     /locus_tag="PLJ64_05685"
     CDS             11889..>12566
                     /locus_tag="PLJ64_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93010.1"
                     /translation="MEIGGVSKGDVLRSLGAAALLLLAALLFAPPAAAALTARDSDPV
                     VLKGSQAGNLAGIDPGRLVAFRWTGSAWDQVPLQVDERKTIDMQSLYPWVTGGANQGY
                     VTNYLVPVNVEVYADPDTRTGADPDATLDPNDEFVFMAFDSGSKAPVDSDPAHVVPRS
                     GVELELDDPLNGDSSFVYLFRSDGNLSPGAGRQYVTYSPSLQNGIGSSYRDNYRYFFP
                     LNTNPPAN"
BASE COUNT         2281 a         4134 c         4097 g         2054 t
ORIGIN      
        1 caggtgtagc cggcgttgtt gaccacgatg tcgatcgagc cgaaagcgtc ctgggcggtc
       61 gcgacgagct ggtcgcaggc atcacctttg gtcaggtcgc cgccgaacac ggcggtttcg
      121 ccatcgatct cggaagcggt ctgctcggcg atgtcggcgt cgaggtcatt gatcagcact
      181 ttggcgccgt gggcggcgaa aagttcggcg gtggcgcggc cgatgccccg ggcagaaccg
      241 gtgacgatcg cgttctttcc ttcaagtagt cccatcgaga gaaacgctcc ttggattggt
      301 tgcaagccgc actatagagc caaaccaccg gccccgaacc tgtgcccccg ggagcctcct
      361 tgcggccccg caacctgccc gcaaagtcgt cgcaaggggc gaattggaac ctgccgtcat
      421 cagcaatccc gattccggag gtcccgccga aatgaaaacc aatcgtcgaa tcagcaaagc
      481 ctgcaccgga gcgaacttcg ttctgaccct cgtcttcgtg gttgcgctct tttcctggtt
      541 cacccaggtt cccgacgcgc aggcggccaa cgtctccttc gtcggcgacg acggcaacgt
      601 ctaccgcacc tcgcccgacg ggacgatcac cgagcaggtg accacggatg ccaccgccga
      661 gacgcgctac gtcactccga cccagaagaa cgacggcacg atcgtggcga tcaagcgggt
      721 cagctcgaac gcgttcgcct acttcctgaa tccggagaac gggcaggtca aggacacctg
      781 gctgctgccg aagaccggag ccggttcctt cgccccgttc aacggcggtg cgatctcgcc
      841 ggacggcggc atgttcgtct acgactggca ttacttcgac tgctggacca acccctgcac
      901 cctcaatcag aaggtttcgt tcatcagcgg tccgggcacg accaacccct gcctggtcaa
      961 ctgccacacc tattacatcc ggccgcgctg gatcccgggt acgccctacg ccggtttcgt
     1021 ggacacggac ttccgccgga tctgggtcca gaaggccggc agcgccgaac cgaacgcctg
     1081 gctcgggttc aatgacccga acgcggggga catgcattcc ttcgatgtct cctcgaacgg
     1141 tctcaccgtg ctggaggtct ccccggaggg atccgaggca gccgagttct ccttctggaa
     1201 caccggtggc accccgccgg ccgggacccc ttcgcaccgc tgctcgatca cggcgatcgc
     1261 ccagaccccg gcctacccgc gcttctcgcc ggacggctcg atgatcacct ggcaggaccg
     1321 tggctcgatc tacgtcgccc cggttccttc gaccaccggc ggcggaagct gcaacctgaa
     1381 cccggtcaag gtcggcgagg gcatggagcc ttccttcggg ctggccaccc tcaagcggga
     1441 cgaaccgacc gacccgaccg atccgaccga ccccacggac ccgaccgacc cgaccgatcc
     1501 cacggatccc acggacccca cggacccgac cgatccgagc gacccttccg acccgtctga
     1561 cccgaccgac cccgggcccg gcacgccgaa ggtcacgctc aaggtgaacc cgggtacggc
     1621 gaaggtcaag gccggccgac cggtgaccct gaccgcggcg gttaccgcca gcggggcgaa
     1681 cgcgccgacc gtcaaggtct gcgccacggt cagcggcaag gcccgcaacg tgatcaagcc
     1741 ggccgcctgc aggacgctcc gcaacgtcgc caggggcagc accgtcagga cttccctggt
     1801 catccgcacc gcgaagaagg cccgtggcag ctacccggtc cgggttgccg cttccggccc
     1861 cggcctcacc ccagtcaact ccaacaacaa ggtccaggtg accaagtgaa cacccgaaac
     1921 cacagcaccc ccgaaatgat caatcgcccg ccggaaagcc gtcccggtga aacccgcctg
     1981 ctcgacggcg gtttccgtgc cggcctggcc ctcttcacct gtctgttcgc cttctggttc
     2041 atgttcgcgg ccgacgatgc cggcgccgcg gcttacaagg gcaagaccaa gggcggcagc
     2101 tcgatcacct tcgccctcaa gggcaaccgg gtgaccggca tccgcaccgt ggtcccgacc
     2161 ctctgcctgg agaccacggg cggatacacc tcccgggccg gcagcgagct gttccagccg
     2221 gtcgcggcgg ggcggatcgg ccggacggtg aagagcaagg cgctccagcc ggccgcgatg
     2281 aacgttggcg ccaaggcgac caagaactac gaggtcacgg tgaacaggcg cggccgcacc
     2341 gtctcgggca agttgaagct caacttctcc ttcctgatcc ccgacctcta cggggcccgg
     2401 atctacatct gctccggagt cacaaccttc agcgccactc cccgctgaac cggccgggcg
     2461 gccccggctt tgcgtcgggg ccgcccggta gtttcggccc gtgaagatga ttcagggcta
     2521 ccgcgagttc atcactcgcg gcaacgtgat cgacctggcc gttgccgtgg tcatcggcgc
     2581 ggcgttcact gccctggtca actcggtcgt cgcggacctg ctgaccccga tcatcgccgc
     2641 gatcatcggc gagccggact tctcggcgct cagcttcacg atcaggggtt ccgagttcac
     2701 ctacggcaac ttcatcaatg ccctgatcgc cttcctctcg atcaccgcgg tgatctactt
     2761 tttcgtggtc ctgccgctca atcacctcaa ggaagcggaa gaggaaacga cccgggaatg
     2821 cccggaatgc ctctcggaga ttccgaacaa ggcaacccgc tgcgcccact gcgcagccga
     2881 ggtaaccccg accgcctgaa ttcaggggat cagcctggag ggggcggcgt ccttgaggag
     2941 gaggttggag agcaggatca ccacgggctt tgtgccgttg ttggcggccc ggtggtggtc
     3001 ggagggctgt tcgatcagcc actggccggg cttgaccttc gcggtctggc cggccttgat
     3061 cagccggacc agcttcggcg cgtctccggc gcctgacatc agcttcgcct ggcccgactg
     3121 gacgtggtag gtgaggacac cggaagcgat gtagccgacc tgggttccct ggtgatagtg
     3181 gagcgggatc tttgcgccgg gctggatcac cacccgggcg aggttgaggg cccggccctt
     3241 gccgccgatc gggttcacga cccgggccag cgagttgtac ttcggggcgg gcggatccga
     3301 ggtggccgag atcgcgatcg cggtgccggc gccgccaacg gcaaccaggg ccaacgcggc
     3361 caggccgatc ctgatcctgc gaccggtcgg gtgactccag cggatcctgt gtccaacctt
     3421 cattgggtga ttcgctccca tgccttgaag ttacggagcc ccaatcctat ttcctgcgaa
     3481 ttattcgtga atttgcaggg aaaaagctgc gaacgactca ggagtacctg agtgcgccgt
     3541 cgtgcatgtc ggcaccgaga agttcacgcc ggtccgagaa gtagttcatg tgaagcaccc
     3601 agggggaggt gtctccggcc agcggaaggc ggtcccgggc ccgcagtatg taaccggaat
     3661 cgaggttgag caaaggcacg gtcttcatcg ggatctccgg agcgaccggc gtgccgcttg
     3721 cggcgcctgc gcgatcgacc tcgccgatga aacggctggc gaaccgggcg atcagctccg
     3781 acttcagggt ccaggaggcg ttcgtgtaac cgaccacgaa gacgaaattg ggaatcccgg
     3841 agagcatcgc tcccttgtag accagtcgtt cggcgggatt gacgggttcg ccgtcgacgg
     3901 tcatcgcgat atcgccgaac agcaggagtt ccagcccggt cgccttgacg atcacgtcgg
     3961 cctcgatctc gccgccctcg cggaggtgga tccggtcgga gctgatccgc tcgatgttcc
     4021 cggtttcgat ccgggcacta tcgccggaaa gggcggcgaa gtaatcgccg tcgggggcga
     4081 aacagactcg ctgggtccag gggtcgtagc ggggattgag atggcgccgc tcgatgtcgg
     4141 gcggcagctg cttgcgggcg ccgctgatga aaagccggcg gaagaagttc ggataacggc
     4201 gggcgagatt gaagttgacg atctccatca ggatcccgaa ccagcggagc aggaaggccg
     4261 cccgccgcgg cgagaaacgg ctacggatcc agcccccgat gccgccggtg gtcgggatcg
     4321 aaagcacgta agtgggagag cgctggagca gggtcacatc ggctccggct tcgaccagag
     4381 ccggcacgac ggtgatcgcg gtcgcgccac ttccgatcac cacgaaccgg cggccgcggg
     4441 ggtcgagtcc ctcgggccag tgctgggcgt ggaagagctc cccttcaaag tcctcttcgc
     4501 cctcgaagac cggcgagaaa cccttgtcgt accggtaata accggtgcag ccaaacagga
     4561 accggcagcg gatccgggag gtggatcccc gggatgaacc attttcggca ctgtgggcga
     4621 ccgtcaccgt ccagcgcgct tcggcgctgt cccagtcggc gccgaccacc ttccggccga
     4681 agcgaatgtg ccggtcgatt ccgtattcgg ccgccgtgtc ctcgaggtac tgctggatct
     4741 ggtccccgtt gccgaagtgg cggtcaccgg gccagggacg aaacgaaaag gcgaaggtgc
     4801 ccatcccgga gtcggaacgg atgcccgggt agcgaaagag atcccaggtc ccgccgatcc
     4861 gcgtgcggtt ctcgagcacg gcgtagctca ggccggggtg gtcgagaccg atctgatggg
     4921 ccatcgagat gccggaaatc ccggcaccca cgatgaccac atcgacttct tcgacaggct
     4981 cggtcactgt ccagagagta cttcccggcc agtgaaccgg ccagtcctac aggccgtagg
     5041 agcgaccaat cacttccagc atcacctcgt cggttccggc accgatccgg ttgaggcgga
     5101 tgtcgcggta cgcccgctcg acttcgtatt ccttcatgta cccgtagccg ccgagcacct
     5161 ggatcgtctg gtcggcgacc tcgcaggcca tctgggcgga gagcagcttg gccatcgaga
     5221 tctcgcggac cgggtactcc ccgttgttga cccgccaggc gaccgagtag gtgaactgct
     5281 tggaagcctc gatcttggtc gccatgtcgg cgatcttgtg gcggatcgcc tggaacttgc
     5341 cgatcggccg gccgaaggcg ttgcgctcgc cggcgtactc gatcgcggtc tcgagcaggc
     5401 gctcggccga ggcattcgag ccggccgcgg cgaccaggcg ctccgactgg agctcccagc
     5461 tgatgtggta gaagcccttg ccgacctgac cgagcacgga atccgccggc acgcggaaat
     5521 cctcgaaggc gagctcgccg gtgtcggaag catgcatgcc catcttttcc aggttctggg
     5581 cgaccgagaa accgggcaga tcgttgccgt cctcgtcgcg gatgtcgatc agcatcaggg
     5641 tgatgccgtc atgaccggcg tcggggtcgg tcttcgtgac cagcaccatg aagtcggccc
     5701 gggcggcgtt ggtgatgaag gtcttggaac cgttgatcac gtactcgtcg ccgtcgagga
     5761 tcgcggtggt gcggatcccg gccacgtcgg agccggcgcc gggctcggtg atgccgagtg
     5821 cgccgatctt ctctccggtg attcccggag ccagatactt ctgcttctgc tcttcggtgc
     5881 cgagcaggtg aatgggcggg aggaccatgt cggtctggac cgcgaagccc atgccgaggc
     5941 caccggagcc cgagtacgaa agtgcctcgg cccgaaccag cgagtagtag taatccccgc
     6001 cctgtccgcc gtactcctcg ggaaagcaga ggccgaggta accgagctcg ccggcccgct
     6061 tcatggccga atcgggccac ttggtctctt cccactccat gcggtggggg tggagctcct
     6121 tcttgaccca gtcggtcatc gactcgcgca ggtcttcgtg ctcctgggtg aagatgaagt
     6181 gcttgcggcc ggcttcatgg tcgggcttga tgacggtttc ggtggacacg cggatctcct
     6241 cggtgaagtt tacgaagtgg acagtagcac ttcctactct ccgctagctg gtatcgtcag
     6301 cggcatgagc caagccactc ctgacttcgg gaagatcagg tacgaggtcg ccgaccatgt
     6361 cgcgacgatc acgctcgacg atcccgatac gcgaaatgcc ctttccgacc agctgctggg
     6421 cgagctgatc tcggccctcc acctggcccg cgaagacacc gaggtacggg tggtcgtgct
     6481 ggcctcctcg catgagacca ccttctcggc cggcggcaac ctggccgcct tcggaggcga
     6541 cgccaacgcc gtccagaagt tcgaaggggc cgagctcttc gtcgagagtt tcaagcttct
     6601 cggtcagctc gggaagccgt cgatcgtcgc cgcccgcggc catgtgctgg ccggctcgct
     6661 gggtcttgcc cttgcctgcg acctgatcgt ggccagcgag gaagcgacct tcggcactcc
     6721 cgagatcaac gtcggcctgt tccccttcat gatcatggcg ctgatctacc gcaacgtgcc
     6781 gcgcaagaag gcgaacgaga tgctgctgct cggcggaagg atggaggcca aggacgcaaa
     6841 ggaggcgggc atcgtcaact tcgtcgccaa gaagaaggac ttcgacctgg tcctcgagga
     6901 ctggaccacg atgctggccc gcaagtcgcc gctgatcatg aagctgggga aggacgcgat
     6961 gtggcggcag atggacatgg acctcgccgc cgcgctcgac tacctccgct cgcaactcgc
     7021 aatcgagctc tccagcgaag acgcaatcga aggcgtcaag gccttcttcg aaaagcgcga
     7081 gccggaatgg aagggacgct gatgagcgag aacgaaaacg ggaccgaggc cgggcagaac
     7141 ctgctgcccg gcacggcgcc gctgaccgcg ctgaccaagc agctccacga gcggcggggc
     7201 aaggcccggc tcggcggcgg cctcgagaag atcgagaagc agcatgcggc aggcaagctg
     7261 accgcccgcg agcgaatcga cctgctggtc gacgagggca gcttcaacga gatcggcatc
     7321 cacggacgcc cgcactactc gcagccggca atgcagtcca aggaggcgcc agccgacgga
     7381 gtggtcaccg gctggggtga cgtcgatggc cggccggtcg cgatcgttgc ctatgacttc
     7441 acggtgatgg ccggctcgat gggccagacc ggcgagatca aggtcaccag gattcgcgag
     7501 atggcactgc agaagcggat gcctctggtc tggctgctcg actcggccgg tgcccggatc
     7561 caggaagcag cgggatcgct cttcgccggt tccggccacc tcttccgcga ggaggtccag
     7621 atgtccggcg tggtgccgat ggttgccgcg atgatgggtc cctgcgccgc cggcacggcc
     7681 tacatccccg gtctttcgga cttcgtgccg atggtggtcg ggcagggcgc gatggccctg
     7741 gccggtccgc accttgtcaa ggccgtcacc ggcgaggaga tcgagatgga ggacctcggc
     7801 ggggccaagg ttcactgccg caagtccggg gtcggagacc tcgaggtgaa ggacgacccg
     7861 gaatgcatcg agtcgatcaa gaagtacctg agctacttcc cgagcaactg cgagcagaag
     7921 cccccggtta ccgaaacggc tgatccggag gaccgcggct cggagaagct cctggagatc
     7981 atccccgatt cgcctcgcac cccgtacaac atgtacgagg tgatcgagga gatcgtcgat
     8041 gacggtgact ggtttgacat caagccgaag ttcgccaagt cgctgatcac ctgcctcgcc
     8101 cggatgggcg gccagccggt cggcatcgtc gccaaccagc ccaaggtact gggcggcgtg
     8161 ctcgacgtcg attccgccga caaggcggcc cgcttcatca acctttgcga cgccttcaac
     8221 atcccgctgc tcttcctgca ggacgtgccc ggcttcctgg tcggctcgaa ggtcgagcag
     8281 caggggatca tccgccacgg ggccaagatg ctctacgcgg tcagccgcgc caccgtgccg
     8341 aagatcaccg tgctggtccg caaggcttac ggcgccggct actacgtgat gtgcggccgg
     8401 gcctacgagc ccgacctgat cgtcgcctgg cccggagccg agatctcggt gatgggggcc
     8461 gagggcgcgg tcaacatcat tggccgctcg gcgatcgagg cggccgatga tccggaggcg
     8521 acccgcgagg cgatgctgaa cgcggtccgc cagcagatcg acccctacat cgcggccggc
     8581 aacgcgatga tcgacgacat catcgacccg cgcgaaacgc ggcagaccgt gatccgcggt
     8641 ctccgcatgg caaaggacaa gcatgtcgag cgtccctgga agaagcacgg ggtaatgccg
     8701 gtatgagcga ggtgatcgag gtcacgattc cccacgagca ggttcagctg aacggcgtga
     8761 tccgcttcga cggctcggtg gtcgagatct tcggcttcaa cgacgtccac tcggtccgga
     8821 tgcacatcca gcagatcgag tttgccgagg tgaaattcga caagggcctg ctggcccagc
     8881 cgcgcatcat gttcaagggc aagtggtcga acctcggcta caaccaggcg atcgatccac
     8941 ccgaccagat caagcccgaa ctcgagaact tcgtcaacgc gatcaacgcc gcgatcggaa
     9001 acgacgccgc gaactacccg aatggagaaa cgcagtgagc aagttcgaag acccggtgat
     9061 catcagcaac tcgatctccg gggtgatcgc caaccgggac cagtgcccgg cgattccgta
     9121 cacccccgag gagtacgcgg ccgaggcgcg ccgctcggtt gacgagggtg ccagccagat
     9181 ccacatccac gcccgcaccc cggacggcac gccctcgtac gagatcgagg acttccaggc
     9241 gatcaccgag gcgatcaagg ccgagtgccc ggaggtgatc atcaactact cgaccggcgc
     9301 gatcggcgtg ccgatcgaga agcggatcgc ctacctcgag gcctgccgcc ccgatgtcgc
     9361 cgcgctcaac atgagctcga tgaactacgc caagtactcg aagcgccgga aggacttcgt
     9421 cttcaaggcg gtcttcgaga actccttcga cacgatcgtc gagttcatca ccgcgatgaa
     9481 cgacctcggc atcaagccgg agcacgagtg ctttgacgcc ggtcacgtcg ccaacctcga
     9541 tccgctctat gacatggaac tgatcaagcc gccctcccag atctccctgg tcatgggtgt
     9601 gaccggcggc atccggccga ccccgcgcaa cgtggcggtc atggccgacc aggtgccggg
     9661 cggccccgag ggcccgaaca actggcaggt gatcggcatt tcccgcgacc agtggaagct
     9721 gctggccgct gcgatcaccc tcggcggcaa tgtgcgggcc ggggtggagg acaacctcta
     9781 cctgcccgac ggcaccatgt gcaagtcaaa cggggagttg atcggcaagg cccggcagat
     9841 ggtcgaggac gccggccgca aggtcgcgac gatcgccgaa gcccgcgaga tgctcggcct
     9901 gaaagcggca tgagtctgcc acttgaagga ataagggtcc tcgacctgac ccggcttctg
     9961 ccgggcggct tctgctcgca gatgctggtc gacttcgggg ccgaggtgat caaggtcgag
    10021 gacaccggcc ccggtgacta catccgctac gccccgccgc tttacgaaag cgatgccgac
    10081 cccgccgagg cgacggcttc cggcctctac ctgtcactca accggggcaa gcgctcgatc
    10141 cgtctcgacc tcaaaagcga ggcgggccgc gaagccttct accgcctcct gccgaccgcc
    10201 gacgtggtgc tcgaaggctt ccggcctggc gtggccgcca agctcggggt cgattacgaa
    10261 accctgagcc gaaagaacga gggcctcgtc tattgctcga tcagcggcta cggccaggac
    10321 ggcccttcac gcgaccgggc cggccatgac atcaactacc tcggttcgac cggcatgctg
    10381 gcgatgaccg gccaggccgg gggcccgccg gtccagccgg ccggccagat cggcgacctc
    10441 ggtggcggcg cgatgaacgc cgccttcggg atcctcgccg cgctgatcga aaagggccga
    10501 agcggcaagg gccagatggt cgacatctcg atgaccgacg gagccttcgc ctggctggcg
    10561 atggcggccg gcgcctactt cgccgggggc gaggtaccac ggcggggaga ggtgatgctg
    10621 ggcggcggca tcgtctgtta cctcacctac gaggcggccg acggctgggt cagctgcggc
    10681 gcgctcgagc ccaagttctg gtgcgccttc tgtgaaggca cgggccacga ggacctgatc
    10741 gagatccagt ttgaacggcc cgggggcgag gcctgggaga aggttgctgc ggtattccgc
    10801 gagcggaccc gggacgagtg gaagtcattc aacgaccagt acgactgctg catcgaaccg
    10861 atcctcgaac tcgatgaagc ggtcgagtcg gatctgctcc gcgaacgcgg aatgatcgtc
    10921 gagtgggaac agccgggcat cggaccggtc aggcagatcg gttcgccgat caagatgagt
    10981 cgcaccccgg ccgcggaacc tggccccgcc ccggcgatcg gtgccgacac ccgcgaggtg
    11041 ctggcctcgg ccggactgag cgcggaggag atcgagggcc tgttcgaatc cggtgtcgcc
    11101 gccgggcccg ggagcggggg agagatggag ttccgggcgt gacccaggcg gcagccgaag
    11161 agaagccgga cgcgccggcc gaacagcttc tgaagatgtc cgggctggtt gaagcctcgg
    11221 gcgtcccggc cccgaccatc aagcattacc tgcgggaggg tctcctgccg gagccggtcc
    11281 ggacttcccg caacatggcc tggtaccggc cggaaacggt cgaacgaatc cgcctgatca
    11341 agcgcctgca ggaggagcgg tacctgccgc tgaaggcgat ccgggcgctc ttcgaggagg
    11401 acccggcgca ggccgaggcg atgctggagg tcgaggaccg gattctcgag cgcgccctgg
    11461 ccggcgagcg gacccgaacc agtgcgaccg aagtcaggaa gtacggcgtg ccgaagaagg
    11521 ttctggagcg actggccgag atcggcatcc tgaccccgaa ctcgcgcggc tactcgccct
    11581 cggacgtgga gatcatcgag gcgatgtcac gcttccgggc cggcggctac gacgaacaga
    11641 tcggcttcac ggtctatgac accctgcgtt acaagcacgc cctcgaagcc ctggtcaagg
    11701 aggaggtcga cgtgctgatg gaacggctcg aaggcgagat cacgccggaa cacgcggtcg
    11761 agctgatcga agccggggcc gaacccctca aggacctgat tgccgccctc cacacgaaat
    11821 tgatggtcga agaggtcgaa cgccgccgct gagcggtttt gcccggaacc ggcgttaccc
    11881 ttcccgtgat ggaaattggg ggagtttcaa agggagatgt gctgcggtcg cttggggcgg
    11941 ccgctctgct gctgctcgca gcgctgcttt tcgctccgcc tgcggctgcg gcgctgaccg
    12001 cccgcgattc cgatccggtc gttttgaaag gcagccaggc cgggaacctg gccgggatcg
    12061 atccggggcg gctcgtcgcc ttccgctgga ccgggtctgc ctgggaccag gtcccgctgc
    12121 aggtcgatga acggaagacg atcgacatgc agagtctcta tccgtgggtc accggtggcg
    12181 ccaaccaggg atacgtgacc aattacctgg tcccggtgaa cgtcgaggtg tacgcggacc
    12241 ccgacacccg gaccggcgcc gaccctgacg ccaccctcga cccgaatgac gagttcgtct
    12301 tcatggcctt cgactcgggg tccaaggcgc cggtcgattc cgatccggcc cacgtggttc
    12361 cccgctcggg ggtggagctc gaactcgacg atcctctgaa cggagactcc tcgttcgtct
    12421 acctgttccg gtccgacggc aacctcagcc ccggtgccgg caggcagtat gtgacctatt
    12481 cgccttccct ccagaacggg atcggctcga gttacaggga caactaccgc tacttcttcc
    12541 cgctcaacac caacccgccg gccaac
//
LOCUS       DAOQKN010000061        12535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_2351_length_12535_cov_5.709054, whole genome shotgun sequence.
ACCESSION   DAOQKN010000061 DAOQKN010000000
VERSION     DAOQKN010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 12535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12535
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2351_length_12535_cov_5.709054"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="PLJ64_05690"
     CDS             <1..244
                     /locus_tag="PLJ64_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93011.1"
                     /translation="NRITPSQRWGIGRVRPHGWRLYFKGGWGSGSGAVDHQIALLRRG
                     RHRIGLSIQTMNNPSHAYGKVTLRGVAARLLRGLPG"
     gene            279..881
                     /locus_tag="PLJ64_05695"
     CDS             279..881
                     /locus_tag="PLJ64_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93012.1"
                     /translation="MTEGNGKFDPEQLGGLDDPVRRYLTRALAPGMTVPDAWSLRMKG
                     SIKVGLRLPFEAGQELDGRSFRWRARVPSNRFPLLEVLDYFDGTGGGMTGRGFGRQLF
                     ADTSADAARSAATRAVMESTMVPATLRPGTGIEWTARSETEIRFRRPAVSLAEEVISG
                     SIPTDARPRSKHRAGARKRTSRAGSSRFSAASTENGNSPE"
     gene            984..2276
                     /gene="obgE"
                     /locus_tag="PLJ64_05700"
     CDS             984..2276
                     /gene="obgE"
                     /locus_tag="PLJ64_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNC93013.1"
                     /translation="MLYDKAKIYVEGGAGGNGSISFRREAHVPRGGPDGGDGGHGGGV
                     VLECDPSRRDLGALRSKHFRAESGHHGEGANKQGARGEDMIIPVPPGTQATSVDGALI
                     DLTEPGQTAVLARGGRGGHGNKRFSTSVRQAPRFAENGTSGESGWVELRLKLLADAGL
                     IGLPNAGKSSLLSRLTRADPKVADYPFTTLEPVLGTIETPDRQVVIADIPGLIEGAAE
                     GAGLGHEFLAHVERCAMLIHVVELAPSDGEPLENYRAVRAELAAHSTGLEKLPELLVL
                     SKSDLWTEADIADQVEEWDRELGESVLGIIPVSSATGAGLERLKESIVRFVPDQVEAP
                     VVTSADGIEVDFEAEHLTYRPKGEGGYSVEEEEEGAFRIHGHGIEVLFERFDTGNEEA
                     LAYLESRLTEIGVMAELKRAGFESGDEIRVGDLEFELY"
     gene            2414..3490
                     /gene="proB"
                     /locus_tag="PLJ64_05705"
     CDS             2414..3490
                     /gene="proB"
                     /locus_tag="PLJ64_05705"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HNC93014.1"
                     /translation="MKLGSSIVSADDGSLREEVLDSVCAQAAALRRAGEPLVMVTSGS
                     IARGIRLLGLQARPRAIDELQAASAVGQGSLFREYESRLETGEAAAAQVLLTASDVAN
                     RTSYVNARQTLRRLLDWGVVPVINENDTTATDEITFGDNDFLAAQVAIMLEARLLVLL
                     TNTDGVFTADPAHDPEARLIDELSSPADLEALEIGDRTSAFGTGGMRSKVAAAGMASG
                     SGIEVRICNGTVDGTLARAAAGEAAGTTFRALKSDAPAFKQWLRYAKPVAGRIVVDEG
                     AARVLRKSGSSLLPVGVTSIEGAFEAGDAVEVVASGTVIGKGIANYSAGEADRIKGMK
                     SVEVLELMPHAAEELIHRDYFVLS"
     gene            complement(3541..5208)
                     /locus_tag="PLJ64_05710"
     CDS             complement(3541..5208)
                     /locus_tag="PLJ64_05710"
                     /inference="COORDINATES: protein motif:HMM:TIGR04534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93015.1"
                     /translation="MALILLGTLALLGTNATQSPAAMPQADLFQDVNPGPSGSNTEAF
                     LPFGNRLWTSANNGKTGYEPWFVGNRKLKLIEDLWPGLDASDPGPGAELNGFYYFPAY
                     DPDHGNELWRFNGNSAELVKDIWPGPEDSTPREFVRVGNRIFFVAHEPTSGRELWQID
                     AGGATLVADINSGTGTSNPSYLTAYLDKVYFSAYTAAHGYELWSSDGTTTAEVGDLRP
                     GSASSDPGSLTVAGGTLFMVANDGTNGYELFKSSGGPATLVADIQPGGNSSSPTSMLP
                     FNNKLIFTANTSVSGSEPWITDGVSVTQIGDLAPGIDSSYASYYSRMNGKVYFVAYLS
                     DPRLYETDGVTASPTTATFPEDASGFYNLAVVGNRLYLSIGTPKTGLEPWVFDGAGAR
                     MIRDILPGPDSSYPTAYAKRGNGVIFAADDGTHGAEPWLLHPDREVKLRPGSSRLGVS
                     KQGFAGLKLTCPANEANGPCISKVTVSTRRPVVFRGKRRKVTLARKSIRVLTGNTGTA
                     RLNFPKPIIRLLLRTPAARRATITVKVHDKAGNKQTVRQGLTLGRPA"
     gene            complement(5214..6890)
                     /locus_tag="PLJ64_05715"
     CDS             complement(5214..6890)
                     /locus_tag="PLJ64_05715"
                     /inference="COORDINATES: protein motif:HMM:TIGR04534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93016.1"
                     /translation="MTLALFAATLVSLLAASPSVGALVPRFALDGSPGPSGSYPFDLT
                     DLNGTLLFSAEIERGNELYATSDGRKIRLVRDLLSGPDDGDPSDLTRFGNFVYFLGYE
                     KNAGYEMWRTNGRSASLFAEPSPGPDSSNIDFLTVADGFLYFSAETPAAGRELWKTDG
                     QATTMIQDLNPGPDGSYPRNLTPFDGQLYFTADTPTEGSELFRTDGNTISLVYDVWPG
                     ASGSEPNDFAVLGSHLYFSAYQPGSSRELWRTDGVTTALWSDINPGAGYGSPGGMLTS
                     GDRLFFSADDGVNGEEPWSTDGLTTSMVADISPGPDDSNPEDMAPFGNGRMIFNAEDP
                     AHGEEPWVADGSSANLLRDIWPGEQDSGSSEFFFRNGTAYFNARDQAHGRELWASNGT
                     GARLVKDILPGTDGSSPRSFTPYRGDVYFSADDGIRGREIWKLVPADRKVTVKGPRKL
                     RFRPSGRADLKLTCPASEATGPCSGQFRLATASKVPFQGKRRSVTLAGGRFRAAPGKS
                     DTVIVRVSNSERRLLKRVAAARNLNVLARVRDGAGNSARVSIPVRAVMPG"
     gene            6902..8278
                     /locus_tag="PLJ64_05720"
     CDS             6902..8278
                     /locus_tag="PLJ64_05720"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HNC93017.1"
                     /translation="MCFQLRASFPRSSASAPTLAPARRAVKRPRSNRYASVMEVTAAD
                     IAADTVRAKSAARAMAAAPTGVKNRALEAIARGLGESVDGILAANASDLADERAQGLS
                     RALTDRLTLTPERVGAMAAGVREVAALDDPVGEVLESRTLENGLELSKIRVPLGVVAV
                     VYEARPNVTIDCAALTIKSGNSILLKGSSYAENTNLALAEVVRSGLAEAGLPEDGVVM
                     SVGGGRESIADLATQDGVVDLLIPRGGEGLKKTLEEVATVPVIFAAAGICHVYVHSDA
                     NLEMARAIAYNAKIQRPGVCNAAETLLVDSAVAAAFLPPALAELSGGGVELVGDESTR
                     ENAGDTPVGEATEVDWDTEYLDLKLAVKVVDGLDEAIEHINRHGSGHSDAIVTDNAQA
                     ADLFAQGVDSAAVYVNASTRFTDGFEFGMGAEIGVSTQKLHARGPVGLKELTTTKYVG
                     RGDGQVRG"
     gene            8280..8885
                     /gene="nadD"
                     /locus_tag="PLJ64_05725"
     CDS             8280..8885
                     /gene="nadD"
                     /locus_tag="PLJ64_05725"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HNC93018.1"
                     /translation="MTRLGVFGSSFNPPTLAHAVLLAEAGWQLALDRIMVVPTGEAWH
                     KDISGAPAAGVRMALAKAAFGGQEGLEVSSLEVDRQGPSYTCDTLEEIHSLNPDSQVF
                     FLSGSDAALGLGEWHRPDRVLELAEFAVAPRSEIARDAVAEVFERLGARDRLEFFAMP
                     RMEFSSTLVRERIASNRPWKHLVPQGVAEMIDNEDLYGSKQ"
     gene            8869..9255
                     /gene="rsfS"
                     /locus_tag="PLJ64_05730"
     CDS             8869..9255
                     /gene="rsfS"
                     /locus_tag="PLJ64_05730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNC93019.1"
                     /translation="MAASSEELSRDIARLLDQKLGEDIVCLDMRELVTYTDYLVICTA
                     RNERQAEAIADEVHLRLKHDNSLLPVNPDRSGDVGWTVLDYLDCVLHIFTEEARQRYD
                     LEDLWHEAPHLDLGLDQSGAKSASAA"
     gene            9281..9353
                     /locus_tag="PLJ64_05735"
     tRNA            9281..9353
                     /locus_tag="PLJ64_05735"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9314..9316,aa:Ala,seq:ggc)
     gene            complement(9497..10006)
                     /locus_tag="PLJ64_05740"
     CDS             complement(9497..10006)
                     /locus_tag="PLJ64_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNC93020.1"
                     /translation="MEAIAPRFSRRLTLVLAAVLLAATFAFTTAAPGADARAGSSTAQ
                     TGVLKGWPTKQRLATEFLRLLKNEDTDGLRRFLDPAFLLQRGDGSYLTKSEYLKDPSE
                     VFAFKVRNIVATHNGDVRVLRFEAKTDQIVNGNPVPGVWIPRLSTFKKTGKVWRLIAH
                     ANFLPPVAT"
     gene            10099..11088
                     /locus_tag="PLJ64_05745"
     CDS             10099..11088
                     /locus_tag="PLJ64_05745"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HNC93021.1"
                     /translation="MSDLRMPAEWAPHEMTLVAWPQREDAWRGTTIEDARDTHAEVVA
                     AIAEFEPVLLVVDPSQAGDARGRVPAENVEIFEAPIDDSWLRDSGPVIVTGEGRRAGI
                     DFTFNSWGEAFLPYDNDAAIAGRILDHLGIEKISSDLVLEGGSIAVDGEGTLVTTGQC
                     LLNPNRNPDLDQAAIEAELASRLDIEMFVWLDQGLIEDEDTDGHVDNICAFIEPGRVL
                     LQTAPEGDPNVPNAQANLARLVDAGLEVVELDLLPRIEGEGDSVVVPYTNFYIANSAL
                     IVPVSGIDPDMDAQALELLGRLYPDREAIGIDGRTLALGGGGIHCITQQIPAA"
     gene            complement(11099..11545)
                     /locus_tag="PLJ64_05750"
     CDS             complement(11099..11545)
                     /locus_tag="PLJ64_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNC93022.1"
                     /translation="MGILEASRVVEIDAPKSECYAIIADLPATPEWQESMISLDVIET
                     NSEGFASLVEIKSDGKVKEVTSLIAFEYQPEEGMSWEQKKGDMKWLKGKWILEELEEG
                     RTRATYWLKADTGRMLGLLIKGPVQDKLKEWLTKDAAEGLKARAES"
     gene            11597..>12535
                     /locus_tag="PLJ64_05755"
     CDS             11597..>12535
                     /locus_tag="PLJ64_05755"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNC93023.1"
                     /translation="MTDLSLNGVHHISCITGDAPGNVEFYAGLLGMRLVKKTVNQDDP
                     SVYHLFYGDEKGSPGFDLTFFEYPGSREGRAGAGMIHRILWRVGSPDSLDYWAARLGQ
                     AGVTTVRSSDEGASFYGIGPTGEGDTLLFQDPEGLTHEILVYDGPDAPLIPGHVDIPA
                     EHAIQGFHGARAFSGNPEVTRQVLGQVLGFTDEGEDHWHLAGEAGDGFEARNGYYFLD
                     EPPAERAIPGAGTVHHIAWASPMDQHDEWREKVAATGLYITPVIDRFYFKALYFREPG
                     GILFEIATIGPGFATDEDPEHLGERLSLPPDFESVRE"
BASE COUNT         2125 a         4015 c         4258 g         2137 t
ORIGIN      
        1 caaccggatc actccgtccc agcgctgggg aatcggtcgg gtgaggccgc acggatggcg
       61 gctctacttc aagggcggct ggggcagcgg cagcggcgcg gtcgaccatc agattgccct
      121 gctccgccgc ggcaggcacc ggatcggact ctcgatccag accatgaaca acccgtccca
      181 cgcatacggc aaggtgaccc tgcgaggagt ggctgcccgg ttgctgcgcg gcctgccggg
      241 ctgactcgcg gcccgtaacc ttccggtcag gtgaggcgat gaccgaggga aacggcaaat
      301 ttgatccgga acaactgggc ggactcgatg atccggtccg ccgatacctg actcgcgccc
      361 tggcgcccgg catgaccgtt ccagatgcct ggagcctccg gatgaagggt tcgatcaagg
      421 tcggtctgcg gttgcccttc gaggccggtc aggaactcga cggcaggtcg ttcaggtggc
      481 gggcccgggt accttcgaac cggttcccgc tcctcgaggt gctcgattac ttcgacggga
      541 cgggcggcgg aatgaccggc cgcgggttcg gccggcaact cttcgccgat acgagcgcgg
      601 acgccgcccg ctcggccgca accagggccg tgatggaatc gacgatggtg ccggccacgc
      661 tgcggccggg caccgggatc gaatggaccg cgcgctcgga aaccgagatc cgcttccggc
      721 gccccgcggt ttcgctcgcc gaggaggtga tctccggatc gatcccgacg gacgcgcgac
      781 cgaggtcgaa gcaccgcgct ggcgcaagga aaaggaccag tcgggcgggc tcctcccgtt
      841 tctctgccgc ttcgacggag aacgggaatt cgccggaatg aaggtgccgg ccaggatgac
      901 cgccggctgg gtcgaaggtg agttcaagcc gttcttctcg gcccggatca cctcggtcga
      961 ggccatctcc gtaccctagg cgggtgctgt acgacaaggc gaagatctac gtggagggcg
     1021 gggctggcgg caacggctcg atctccttcc ggcgcgaggc ccatgttccc cgcggcgggc
     1081 ccgatggcgg cgacggcggc cacggaggcg gggtggtgct cgaatgcgac ccctcccggc
     1141 gagacctggg cgcgctccgt tcgaagcact tccgggccga gagcggtcac cacggcgagg
     1201 gtgccaacaa gcagggcgcc cgcggcgagg acatgatcat cccggtgccg cccggcaccc
     1261 aggcgacttc ggttgacggc gccctgatcg acctgaccga accgggccag accgcggtcc
     1321 tggcgcgggg cgggcgtggc ggccacggca acaagcgctt ttcgacctcg gtccgtcagg
     1381 ctccgcgctt tgccgaaaac gggacctccg gagaatcggg ctgggttgaa ctgagactga
     1441 agctgctggc cgacgccggc ctgatcggcc tgcccaacgc cggcaagagc tcgctgctct
     1501 cccgcctgac cagggccgac cccaaggttg ccgactaccc gttcaccacg ctcgagccgg
     1561 ttctcggaac gatcgaaacc ccggaccgcc aggtcgtgat cgccgacatc ccgggactga
     1621 tcgagggtgc cgccgagggg gccgggctcg gtcacgagtt cctcgcccac gtcgaacgct
     1681 gcgcgatgct gatccacgtc gtcgagcttg ccccctccga cggtgagccg ctggagaact
     1741 accgggcggt acgggccgag cttgccgctc acagcaccgg tctggagaag ctgcccgagc
     1801 tgctggtgct ttcgaagtcc gacctctgga ccgaggccga catcgcggac caggtcgagg
     1861 aatgggatcg cgagctcggc gaatccgtgc tggggatcat tcccgtttca tctgcgaccg
     1921 gcgccggcct ggagcggctg aaggagtcga tcgtccgctt cgtgcctgac caagtcgagg
     1981 cgccggtcgt aacctcggcc gacgggatcg aagtcgactt cgaagccgaa cacctcacct
     2041 accggccgaa gggggagggc ggctactcgg tcgaggagga agaagaaggt gccttccgga
     2101 tccacggcca cggcatcgag gttctcttcg aacgcttcga caccggcaac gaggaagcac
     2161 tcgcctacct cgaatcccgt ctcaccgaaa tcggcgtgat ggccgaactg aaacgggccg
     2221 ggtttgagtc cggagacgag atccgggtcg gagacctcga gttcgagctt tactgactta
     2281 actgcagtcg ggccggtctt cgccggccct ccaatctcca cccgcccctc ccatcaggag
     2341 caaaggatgg ttgggagggg ctctgccggt cgtggcatca gcctctaggg ttggtgggga
     2401 gatgactttg gtggtgaaac ttggatcttc gatcgtttcg gcggatgacg ggagcctgcg
     2461 cgaggaggtg ctcgattccg tctgcgccca ggcggctgct ctgcggcggg ccggggagcc
     2521 gctggtcatg gtcacctccg ggtcgatcgc ccgggggatc cggctgctcg gtctccaggc
     2581 caggccgcgg gcgatcgacg agctgcaggc cgcttccgcg gtcggccagg gctcgctctt
     2641 ccgcgagtac gagtcccggc tcgagacggg ggaggcagcg gccgcccagg tgctgctgac
     2701 cgcatccgac gttgccaacc ggaccagtta cgtgaacgcc cgccagaccc tgcgccggct
     2761 gctcgactgg ggtgtggtcc cggtcataaa cgagaacgac accaccgcca ccgacgagat
     2821 caccttcggg gacaacgact tcctcgccgc ccaggtggcg atcatgcttg aggccaggtt
     2881 gctggttctc ttgaccaata ccgacggcgt cttcaccgcc gatccggccc atgaccccga
     2941 ggccaggctg atcgacgaat tgtcaagccc ggccgacctt gaagcgctgg agatcggcga
     3001 ccgcacctcg gctttcggca ctggcggaat gcgcagcaag gtcgccgcgg ccgggatggc
     3061 cagcggctcc gggatcgaag taaggatctg caacggcacc gttgacggaa cccttgcgcg
     3121 cgctgccgcc ggcgaggcgg cgggcaccac cttccgcgcg ctgaagagcg acgcgccggc
     3181 tttcaagcag tggctcaggt acgccaagcc ggtggccggc aggatcgtcg tcgacgaagg
     3241 cgccgcccgc gtgctccgca agagcgggtc aagcctgctt ccggtcggtg tgacctcgat
     3301 cgaaggtgct ttcgaggccg gggacgcggt tgaggtggtt gcgtccggaa cggtgatcgg
     3361 caagggcatc gccaactact cggccggcga ggccgaccgg atcaagggca tgaagagcgt
     3421 cgaggtgctc gaactgatgc cccacgccgc ggaagagctg atccaccgcg attacttcgt
     3481 cctttcttaa ctgcaagtcc cttcctctca cattcagggt tagaaggaag gggctctgtc
     3541 tcaggcggga cggcctagcg tgagaccctg ccggacggtc tgcttgttgc cggctttgtc
     3601 gtgaaccttg accgtgatcg tggcgcggcg ggcggcgggg gtgcggagca gcagccggat
     3661 tatcggcttc gggaagttga gacgggcggt gccggtgttg ccggtcagaa cccggatcga
     3721 cttgcgggcc agggtcacct tgcgccgctt gccgcggaag acgaccggcc gccgggtcga
     3781 gacggtcacc ttcgagatgc acggtccgtt cgcctcgttt gccgggcagg tcagcttcag
     3841 tccggcgaag ccctgcttcg agacgcccag ccttgaactt cccggcctga gcttcacctc
     3901 ccggtcgggg tggagcagcc agggctcggc cccgtgggtg ccgtcatcgg ccgcgaaaat
     3961 caccccgttg ccccgtttgg cgtacgcggt cggatacgag gaatccggac cgggcaggat
     4021 gtccctgatc atgcgggcgc cggctccgtc gaaaacccag ggttccaacc cggtcttcgg
     4081 agtgccgatc gacaggtaga gccggttgcc gaccacggcc aggttgtaaa agccggaggc
     4141 gtcttccggg aaggtggcgg tcgtgggact ggccgtgaca ccgtcggtct cgtacagccg
     4201 gggatcggaa aggtaggcga caaagtagac cttgccgttc atccggctgt agtagctggc
     4261 gtaggaagaa tcgatccccg gggcaaggtc gccgatctgg gtgacgctga cgccatcggt
     4321 gatccagggt tcggagccgg acacactcgt gttggccgtg aatatgagtt tgttgttgaa
     4381 gggcagcatc gaggttggtg aagatgagtt gccgccgggc tggatgtcgg cgaccagggt
     4441 ggccggcccg ccggagctct tgaagagttc gtagccgttg gtgccgtcgt ttgcgaccat
     4501 gaacagcgtt ccgccggcaa ctgtcaggga acccgggtcc gaggaagcgg aaccggggcg
     4561 gaggtcaccg acttcggccg tggtggttcc gtcgctcgac cacagttcgt atccgtgggc
     4621 ggcggtgtag gccgagaagt aaaccttgtc caggtacgcg gtcaggtaac tggggttcga
     4681 agtgccggtt cccgaattga tgtcagcgac cagggttgct ccgccggcat cgatctgcca
     4741 gagctctcgg ccgctggtcg gctcgtgggc cacaaagaag atgcggtttc cgacccgcac
     4801 gaattcccgg ggggtcgaat cttcggggcc cggccagatg tccttgacca gctccgcgga
     4861 atttccgttg aagcgccaga gctcgttgcc gtgatcggga tcgtaggcgg ggaagtaata
     4921 gaagccgttc agctcggctc ccgggccggg gtcggaggcg tccaggcccg gccagaggtc
     4981 ctcgatcagc ttcagcttcc gattgccgac aaaccacggc tcatagccgg tcttgccatt
     5041 gttggcagag gtccagagcc gattcccgaa cgggagaaac gcctcggtgt tcgagccgct
     5101 ggggccggga ttcacatcct ggaagaggtc ggcctgcggc atcgcggccg gggactgggt
     5161 cgcgttcgtg cccagcaggg cgagcgtgcc gagaagaatg agggccaccc gcttcatccg
     5221 ggcatcaccg ccctgaccgg gatgctgacc cgggccgagt tcccggctcc gtccctgacc
     5281 cgggcgagca cgttcagatt ccgcgcggcg gcaacccgct tcagcaatct ccgttcagag
     5341 ttgctcacgc ggacgatcac cgtgtccgac ttgcccggtg cggccctgaa gcggcccccg
     5401 gccagcgtga ccgaccttcg tttgccctgg aagggaacct tcgaggcggt cgcgagcctg
     5461 aactggccgg agcagggtcc ggtcgcctcg ctggccgggc aggtcagctt caggtcggct
     5521 cgcccgctgg gccggaaacg gagcttgcgg ggccccttca cagtgacctt cctgtcggcc
     5581 ggcaccagct tccagatttc gcgtccgcgg atgccgtcgt cggcgctgaa gtagacgtcg
     5641 ccccggtaag gggtgaagga acggggagag gagccgtcgg ttccgggcag gatgtccttg
     5701 accaggcgcg cgccggtgcc gttgctcgcc cagagctccc ggccgtgagc ctggtcccgg
     5761 gcgttgaaat atgcggtgcc attgcggaag aagaattcgc tcgacccgga gtcctgttcc
     5821 cccggccaga tgtccctgag caggttcgcc gacgatccgt cggcgaccca tggttcttcg
     5881 ccgtgtgccg gatcctcggc gttgaagatc atccggccgt tgccgaaagg tgccatgtct
     5941 tcagggttgg agtcgtcggg tccgggggag atgtcggcaa ccatcgatgt ggtgaggccg
     6001 tccgttgacc acggttcttc gccgtttacg ccgtcatcgg cggagaagaa aagccggtcg
     6061 ccggaagtca acatgccgcc cgggctcccg tatccggcgc cgggatttat gtcggaccag
     6121 agcgccgtgg tcacaccgtc cgtccgccag agttcccggc ttgaccccgg ctggtaggcg
     6181 ctgaagtaga ggtgcgaacc gagcacggcg aaatcgttgg gttcgctgcc gctggctccg
     6241 ggccagacgt cgtagacgag gctgatcgtg ttgccgtcgg tccggaagag ctcgctgcct
     6301 tcggttggcg tatcggccgt gaagtagagc tggccgtcga agggagtcag gttccggggg
     6361 taggagccgt ccggcccggg attcaggtcc tggatcatcg tcgtcgcctg gccgtcggtc
     6421 ttccagagct cccttccggc agccggggtt tcggcggaga agtagaggaa accgtcggcc
     6481 acggtcagga agtcgatgtt cgatgagtcc ggtccggggg aaggctcggc gaacagcgaa
     6541 gcactcctgc cgttggttct ccacatttcg tatccggcgt tcttctcgta gccgaggaag
     6601 tagacgaagt tgccgaatct ggtgaggtcg gaaggatccc cgtcgtccgg tccggaaagc
     6661 aaatcccgga ccagcctgat cttcctgcca tcactggttg cgtacaactc attgccgcgc
     6721 tcgatctcgg ccgaaaacag cagggtgccg ttgaggtcgg tcaggtcgaa cggataagac
     6781 ccgctgggcc cgggactgcc atcaagggca aagcggggaa caagggcgcc caccgagggg
     6841 gaggccgcca gcagcgacac caaagtggcg gcgaacaagg ccagcgtcaa ccggaccttc
     6901 aatctgtttc caactccgtg caagcttccc ccgtagcagt gcgtctgccc cgacgctagc
     6961 gcccgcacgg cgcgcggtca agcggcctcg atcgaacagg tacgcttcag ttatggaagt
     7021 gaccgccgcc gacatagccg ccgatactgt cagagccaaa agcgccgccc gcgccatggc
     7081 cgcggctccg accggggtca agaaccgggc gctcgaggcg atcgcccgtg gtctcggtga
     7141 atcggttgac gggattctcg ccgcgaacgc ttcagatctg gccgacgaaa gggcccaggg
     7201 gctttccagg gcgctgactg accggcttac gctcacgccc gagcgcgtgg gagcgatggc
     7261 cgccggggtg cgggaggtcg ctgccctcga cgacccggtc ggggaagtgc tggaatcgcg
     7321 caccctcgag aacgggcttg agctgagcaa gatcagggtg ccgctcggcg tcgtcgccgt
     7381 ggtttacgag gcgcggccga acgtgaccat cgactgcgct gccctgacca tcaagtcagg
     7441 caactcgatt ctgctcaagg ggtccagcta cgccgagaac acgaacctgg cgctggccga
     7501 agtcgtgcga tcaggcctgg ccgaagccgg cctgcccgag gacggcgtcg tgatgtcggt
     7561 cggaggcggc cgcgagtcga ttgccgacct cgccacccag gatggcgtgg tcgacctgct
     7621 gattccccgc ggcggtgaag ggctgaagaa gacgctcgaa gaggtggcga ccgtaccggt
     7681 gatcttcgct gcggccggga tctgtcatgt ctacgtccac tccgacgcga acctggagat
     7741 ggcccgggcg atcgcctata acgcaaagat ccagcgcccc ggcgtctgca acgcggccga
     7801 gaccctgctg gtggacagcg cagtcgccgc tgccttcctg ccgcccgccc tggccgagct
     7861 ttccggcggc ggagtcgaac tggtcgggga cgaatcaacc cgcgagaacg ccggggacac
     7921 accggtcggc gaagcgaccg aggtcgattg ggacaccgag tacctcgacc tgaaactggc
     7981 cgtgaaggtg gtggacgggc tcgacgaagc gatcgagcac atcaaccgcc acggcagcgg
     8041 acactccgac gcgatcgtca ccgacaacgc ccaggcggcc gatctttttg cccagggtgt
     8101 cgattcggct gccgtctacg tgaacgcttc gacccggttc accgacggat tcgaattcgg
     8161 tatgggagcc gaaatcggcg tttcgaccca gaagcttcac gcccgcgggc cggtcggcct
     8221 caaggaactg accacgacca agtacgtggg ccgcggcgac ggccaggttc gcggctgaag
     8281 tgacccggct cggggttttc ggctcttctt tcaatccgcc gacgctggcc cacgcggtgc
     8341 tgctcgccga agccggctgg cagctcgccc ttgaccggat catggtcgtg ccgaccgggg
     8401 aggcctggca caaggacatt tccggggcgc ccgccgccgg ggtccggatg gccctggcga
     8461 aggcagcctt cggcggccag gaagggcttg aagtttcatc gctcgaagtg gaccggcagg
     8521 gtccgtccta cacatgtgac acccttgagg agatccattc tttgaatccg gacagccagg
     8581 tcttcttcct ctcgggctct gatgccgccc tggggttggg ggagtggcat cggcccgacc
     8641 gcgtgcttga gctggcggaa tttgccgtgg caccgcgttc agagatcgcc cgggacgcgg
     8701 tggcggaggt cttcgagagg ctcggagcgc gagaccggct cgagttcttc gcgatgccga
     8761 ggatggagtt ttcatcgact ttggtccggg agcggatcgc ctcgaaccgg ccctggaaac
     8821 acctcgtacc gcaaggggtg gcggaaatga tcgacaatga ggatctgtat ggcagcaagc
     8881 agtgaagaac tgagccggga tatcgcccgg ctgctggatc aaaagctcgg cgaggacatc
     8941 gtctgtctcg acatgcgcga gctggtcacc tacaccgact acctggtgat ctgcaccgcc
     9001 cgcaacgaac ggcaggccga ggcgattgcc gacgaggtcc acctgcgcct caagcacgac
     9061 aactcgctgc tgccggtcaa tccggaccgc tccggtgacg tcggatggac cgtgctcgac
     9121 tacctcgact gcgtcctcca catcttcacc gaggaagcca ggcagcgcta cgacctcgaa
     9181 gacctctggc acgaagcgcc ccacctcgat ctcggtctcg accagtccgg ggcgaagtcc
     9241 gcctcggccg cctgatcccg gtaaactcct tcgtccctct ggggctatag ctcagctggc
     9301 agagcgcttg catggcatgc aagaggtccc cggttcgatc ccgggtagct ccactcgtaa
     9361 gttctcctcg aagtccccgc gtacggcggg cggtccccac ggagccgttc gcgcggctcc
     9421 acgggcttcc acgcgcaaag gtgccgcaca tccagccccg tgacggggct gattttgcgg
     9481 cacctcggct gttcctctag gtcgcgacgg gcgggaggaa gttggcgtgg gcgatcaggc
     9541 gccagacctt gccggtcttc ttgaaggtcg aaagccgggg gatccaaacc cccggaaccg
     9601 ggttgccgtt gacgatctgg tcggtcttcg cctcgaagcg aagcaccctc acgtcaccgt
     9661 tgtgggtggc gacgatgttg cggaccttga aggcgaagac ttccgaagga tccttgaggt
     9721 attcggactt ggtcaggtaa ctgccgtcac cgcgctggag gaggaaagcg gggtcgagga
     9781 aacgacggag cccgtcggtg tcctcgttct tcagcagtcg cagaaactcg gtcgcgagcc
     9841 gttgcttggt cggccagccc ttcagcacac cggtctgcgc agtcgacgat ccggccctgg
     9901 cgtcggcgcc gggcgcagcg gtcgtgaagg cgaaggtcgc ggccagcaga acggctgcga
     9961 ggacaagtgt cagtcggcgc gagaagcgcg gggcgatggc ttccatgtgt ctccctgggt
    10021 tggtcaagcg aaggtaactg cggcgagcct acccggcggg gtgaaaacag gcaagcgggc
    10081 cgggggtaga ctcgcgccat gagcgatctg cgcatgcccg ccgaatgggc gccccacgaa
    10141 atgaccctgg tcgcctggcc gcagcgggaa gacgcgtggc ggggaacgac gatcgaggac
    10201 gcccgtgaca cccacgcgga agtcgtggcc gcgatcgccg agttcgaacc ggtgttgctg
    10261 gtcgtcgacc cctcccaggc gggcgacgcc cgcgggcggg tgccggcgga aaacgtggag
    10321 atcttcgaag ccccgatcga cgattcctgg ctccgggatt ccgggccggt catcgtcacc
    10381 ggcgaaggcc gccgggccgg aatcgacttc accttcaact cctggggtga agcgtttctc
    10441 ccttacgaca acgacgcggc gatcgccggt cgcatcctcg atcacctggg aatcgagaag
    10501 atcagctccg acctggtcct cgagggcgga tccatcgcgg tcgacgggga ggggacgctg
    10561 gtcacgaccg ggcaatgcct gctcaacccg aaccggaatc ccgatctcga ccaggcggcg
    10621 atcgaagcag aactggcttc cagactcgat atcgagatgt tcgtctggct cgaccagggc
    10681 ctgatcgagg acgaagacac cgacgggcac gtcgacaaca tctgcgcctt catcgaaccc
    10741 ggccgggttc tgctccagac cgcgccggaa ggcgatccga acgtgccgaa cgcccaggcc
    10801 aacctcgccc gactcgtcga cgccggactc gaggtggtcg aactcgatct gctcccgcgg
    10861 atcgaaggcg agggcgactc ggtggtggtg ccgtacacga acttctatat cgccaacagc
    10921 gccctgatcg tcccggtcag cggcatcgac ccggacatgg acgcccaggc gctcgagttg
    10981 ctcggtcgcc tctaccctga tcgggaagcg atcgggatcg acggacgcac cctcgccctc
    11041 ggcgggggcg gcatccactg catcacgcag cagatcccgg ccgcctgatc gcggctgtct
    11101 agctctcggc gcgggccttg aggccttcgg ctgcgtcctt ggtcagccat tccttcagct
    11161 tgtcctggac cggacccttg atcagcaggc ccagcatgcg accggtgtcg gccttgagcc
    11221 agtaggtggc gcgggtcctg ccttcctcga gctcctccag gatccacttg cccttcagcc
    11281 atttcatgtc gcccttcttc tgttcccagc tcatgccttc ctcgggctgg tattcgaagg
    11341 cgatcagcga agtgacttcc ttgaccttgc catcggactt gatttccacg agcgaggcga
    11401 agccctcgga attcgtctcg atgacgtcaa gcgaaatcat cgattcctgc cactcgggag
    11461 tggcgggaag gtcggcaatg atcgcgtagc actcggactt gggggcgtcg atctccacga
    11521 cgcgtgaagc ttcaaggatt cccatgttcg ggatgctatc ccggaccggg gatgccagcc
    11581 aagtacgctt ggcttcatga ccgatctcag cctgaacggc gttcatcaca tcagctgcat
    11641 caccggcgac gcaccgggaa acgtcgagtt ctacgccggc ctgctcggca tgcggctggt
    11701 caagaagacg gtcaatcagg acgacccctc cgtctaccac ctcttctacg gtgacgagaa
    11761 gggctcgccc ggtttcgacc tgaccttttt cgaatacccc ggatcgcgcg aggggcgggc
    11821 gggagccggg atgattcacc gcattctctg gcgggtcggc tcccctgatt cactcgatta
    11881 ctgggccgcg aggctcgggc aggccggggt cacgacggtc cgcagttccg acgaaggcgc
    11941 ctccttctac gggatcggtc cgaccggtga gggcgacacc ctgttgtttc aggacccgga
    12001 agggctgacc cacgagatcc tcgtctacga cggaccggat gcgccgctga tcccgggcca
    12061 cgtagacatc ccggccgagc atgcgatcca gggcttccac ggagcccgcg ccttctccgg
    12121 caaccccgaa gtgaccaggc aggtgctcgg acaggtgctt ggcttcaccg atgaaggcga
    12181 ggatcactgg catctggccg gcgaggcggg cgacggtttc gaggcccgca acggctacta
    12241 cttcctcgac gagccgccgg cggagcgggc gatcccgggc gccggaacgg tgcatcacat
    12301 cgcctgggcc tcgccgatgg accagcacga cgagtggcgg gagaaggtcg ccgcgaccgg
    12361 gctctacatc accccggtga tcgaccgctt ctacttcaag gcgctctact ttcgcgagcc
    12421 gggcggcatc ctcttcgaaa tcgccacgat cggccccggt ttcgccaccg acgaggaccc
    12481 ggagcatctc ggcgagcggc tctcgctgcc ccccgatttc gaatcggtcc gcgag
//
LOCUS       DAOQKN010000062        12453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_2370_length_12453_cov_6.290773, whole genome shotgun sequence.
ACCESSION   DAOQKN010000062 DAOQKN010000000
VERSION     DAOQKN010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 12453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12453
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2370_length_12453_cov_6.290773"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="PLJ64_05760"
     CDS             <1..818
                     /locus_tag="PLJ64_05760"
                     /inference="COORDINATES: protein motif:HMM:NF017013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MOFRL family protein"
                     /protein_id="HNC93024.1"
                     /translation="IASGMPITAINTVRKHVSGIKGGRLAELIAPARIVNLTISDVAG
                     DPQDVLADLTTQDTSTPEQALSILRRFDLIDEVPGSIISHLGSPAALSPDLGGIEIST
                     AMLVNGESACRAMADAAGAIGLRPLLVGTAVEGEAVETGKTIASLARAEPAGTALIGC
                     GGESTVSLGRSEPGLGGPNQEAAIAASAELEGRQVAAVFLDTDGSDGSTRFAGAICDG
                     RTAERARELGLDLESALASHAASEPLRALEDLIDTGPTGTNVNDLFVAVTGPI"
     gene            complement(843..1136)
                     /locus_tag="PLJ64_05765"
     CDS             complement(843..1136)
                     /locus_tag="PLJ64_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93025.1"
                     /translation="MSEPEAGEGDESTEVEQSPVPARGERFGFDLGNPVERESAAFSW
                     LIVVIIAAVAVGAVGKLVSPLAAVILVLILLGIVSVPIFRGLRHGLGSPDDDE"
     gene            complement(1144..1770)
                     /locus_tag="PLJ64_05770"
     CDS             complement(1144..1770)
                     /locus_tag="PLJ64_05770"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HNC93026.1"
                     /translation="MESASIAAHGHVDDHHHGPPEAHQSARIDRTILGILLFILSEVM
                     LFGAFFAAYFFLRVVADPASWPPEGVELPVLIAGINTCILVSSSFTVHYALEAIKHNN
                     HRGLKLGLTTTWLLGATFLFIQINEYIHIGFTPRDGAFGSIFFSLTGLHGAHVFVGLL
                     LLTFANIRAYRGHFGPEQKDHLGVEVPGIYWHFVDIMWIIVFLAVYII"
     gene            complement(1773..2678)
                     /locus_tag="PLJ64_05775"
     CDS             complement(1773..2678)
                     /locus_tag="PLJ64_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93027.1"
                     /translation="MSKPLLIFLNEVAGGRKLLEAAREKAADASYVVVVAPQNQPSVG
                     QLFDPEERAEAARARVDVTLAVLDEFGIEAIGEVLDPEYDLALGDAIRSHRPGEVLLS
                     CTYDFRFGLTRRDLLSWAEITYGDVTNITHIPVRIEDDSISLNVTHTLVVANRTLTSE
                     DLQERLLDRSGDRPHRYTIIAPAADDVSEATVARNLATVMAKLYHADIDATGQPMSPD
                     PFDAVKNAIEHYRVDEILISTLSGEESHWLKEDLIGRVREITEKPVVHHEAGRPPEAV
                     AAVVAEGEAPEAAGAEDSTAEENEA"
     gene            complement(2675..4588)
                     /locus_tag="PLJ64_05780"
     CDS             complement(2675..4588)
                     /locus_tag="PLJ64_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNC93028.1"
                     /translation="MAAAIRQPGWYRAAIGTVLGAAFGFFLVVGLRLLSGLDAFQTEQ
                     TGYPQVIVPLITTPFGFLFGFGAFDYWFRWAAGKPTIPEDHSGHGATRWQDYFTFNTD
                     HKVIGTQYVVTTLFFFLIGGLLALLMRAELAQPGGQIVAANTFNGLFSTHAVVMIFLF
                     IIPIFAGLANYVLPLMIGAPDMAFPRLNALSFWLLPIAGIIFLGSFLAPGGSFDAGWT
                     GYAPLSTGAPLGQSFFNIGVQFAGASSIATALNFLVTIITMRAPGMNLWRMPLLVWAN
                     LSTSLLVVGATPFVAGAQFMVFFDRILGMNFFNFLEGGDVISYQHIFWFYSHPAVYIM
                     MLPGFGIISEIISTHARKPVFGYRLMALALIGIVVLSYSVWAHHMFVAGMFSWLRVPI
                     MITTMLIAVPTGIKVFSWLGTLFYGKIHTYSTAMLFSLAFIITFTVGGISGVMTAMIP
                     LDIHVSDTYFIVAHIHFVLFGGSVFTIFAGIYHWFPKMTGRMYDERLGRIHFWGTLVG
                     TWMTFVPMHWIGMDGMPRRVADYATQYGNWNLLISVSAFVLGAVQLIFLYNMIVSWRF
                     GPKAGNNPWRANSIEWQVSSPPPVFNFDEIPRVVGGPYEYGTPGARHAIMGGEGEGAE
                     VPETQPSTVTAPS"
     gene            complement(4627..5442)
                     /gene="coxB"
                     /locus_tag="PLJ64_05785"
     CDS             complement(4627..5442)
                     /gene="coxB"
                     /locus_tag="PLJ64_05785"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HNC93029.1"
                     /translation="MTRLPILKMVLAVIVLGVVQSVVLLQVDWFGDAASTAAGPIDTL
                     LDVTIVVSSFVFAIVCVALGYALIKWRAKPGDESDGLPIHGNTKLEIVWTIIPIIMVL
                     GLAGYSWAVLDDIEKTDADAIHINAYAQQFAWTFGYPESGNKWSEGELHVPVDRQIII
                     DLQAQDVLHSFWVPEWRIKKDAVPGLETDAIITPDKEGTYQLICAELCGIGHTTMRAI
                     VVVESEAKYQAWLKQQTTEVPDDLLLTTDQALEKQKQQQEQSGGIEGSGVKEQ"
     gene            complement(5496..7046)
                     /locus_tag="PLJ64_05790"
     CDS             complement(5496..7046)
                     /locus_tag="PLJ64_05790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNC93030.1"
                     /translation="MKILIFHGYLLRGTGSNIYNANLARALAGLGHEVHLLCQDREAG
                     SLDWVNAVGTWPDAEGPESIGELQFEQIRQPGGEGSVTVYRPPIGKLLPVYVEDPYEG
                     FEARAFPRLTDQEIDFYVGSNVAAVEAVERQIGGSDAALANHLIMGPVILARAGVRPY
                     AVKNHGSDLEYTIKPNPRFIPWAAEGVESAAAVLVGSHHTAASMWEALPGLGLEEKTG
                     FGPPGVDTQAFAPLKPGEHEGRIAALRNEIANEPPSTTFGRDADEAVAALDAFAAAEG
                     PRVIFVGKLIVSKGVDLIVAAWPLIHRQNPGARLMLVGFGAYEMGLRALVTALTDGDI
                     QSARDIAAIGRGLEGGEEEPLSYLTDFFSDLPAGYEEAGVQAAGSIAFAGRLDHDEVA
                     RVVPAADSMIVPSTFPEAFGMVAAEAAASGVLPVCAEHSGLAEVTATLAEEVPAVESM
                     IGLPLGPGAVAELAGKVNRWLALDPAERLEIGQNIAESADRNWSWQGVAKDVISASAG
                     QVRKVVQS"
     gene            7068..7340
                     /locus_tag="PLJ64_05795"
     CDS             7068..7340
                     /locus_tag="PLJ64_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93031.1"
                     /translation="MSTESVSNEQIYRLRAAASGREAIAPVKDGVVYRDAETGEELEP
                     VAAVLPNTGEGSSLPRTPVNLRVCRRCEQLTPRDLDTCEFCGLPAS"
     gene            complement(7417..8265)
                     /locus_tag="PLJ64_05800"
     CDS             complement(7417..8265)
                     /locus_tag="PLJ64_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93032.1"
                     /translation="MAEPRSHFVSRPALRLGALVAATLGLFACLVLAGAFGDADGKSN
                     RRVVLGATRDKVTPNCGTRSGARACIAEGKVTGFQVFQKGVTGKNFVVPFKKGKVVAW
                     SIQLSNPLKEDSRRFGPAQQPYFNKLFGSPSKARISILRPIKKGARYPPRYKLIRASG
                     TQKLNRYFGSEIKFAIKPLKVIRGDVVALTIPTWAPAFWVPMACSPSDRGTANAARCA
                     DARKANTWRGSRGPGQCAIGTNAFDQPNEALRNSHPQQKVNSIKRYACYYDAGRLLYK
                     ATVVAP"
     gene            complement(8382..9221)
                     /locus_tag="PLJ64_05805"
     CDS             complement(8382..9221)
                     /locus_tag="PLJ64_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93033.1"
                     /translation="MLRSPLTTDGLRVARVFAVLSGLAALILIAFAPHAEAKSTQRIE
                     LGARNINTLQPNCGRDFTRDCTVEGKVTAFQSLSARYPGRNFVVPFNGKLVAWSISLS
                     NPTRVEIDNNGAQLPSFNLIFGSPAQANIAILRQVEKRKKGGPRFKLVRKSPIQILNP
                     YFGTKVTFALDVPLNVYEGNVVALTIPTWAPALWKPKACNQTDFGILDPGRCDQAEKA
                     YTWRGSRVARGEPETCGLSNDGGPNEQLQKTAPQTHLDSVRRYGCYYGGNVLLYSAII
                     VGR"
     gene            complement(9295..9513)
                     /gene="infA"
                     /locus_tag="PLJ64_05810"
     CDS             complement(9295..9513)
                     /gene="infA"
                     /locus_tag="PLJ64_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNC93034.1"
                     /translation="MAKEEKIEMEGEITEALPNTMFRVKLDNDHEVLGHISGKMRRHY
                     IRILPGDRVKIELSPYDLTRGRITYRYK"
     gene            complement(9533..10978)
                     /locus_tag="PLJ64_05815"
     CDS             complement(9533..10978)
                     /locus_tag="PLJ64_05815"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNC93035.1"
                     /translation="MTRVLGLEVLGQRALGAPRVDRVREALAGTPAWLVGGAVRDIAL
                     GRETNDLDLAVTGSPEVPARAIAKALDGIAFELSEEFPAWRAKDRDDAWQVDVAELRA
                     PDIERDLELRDFTVGAVAVSLENGEAIDPLGGLGDLAAGTIRVCGPTSFSDDPLRLMR
                     AARLVAQFGWEIEEATAELARQSAPRAGDPAGERTLAELILLIGARDPLAGLAAMDRI
                     GLFDWLLPEIGDLHDVVQGPNHHLDVYGHTMEVVEGILRIESDLETFTGERAPEVSAY
                     LSRPLADGATRGAALRLGALFHDCAKPQTRSEEGGFVSFRGHDRLGAEQIDSRFRSLR
                     ASRKLSSYIADLTRHHLILGFLVNQMPLDKRQVFAYLDHTDPVSIDVTLLTVADRLAA
                     RGTAEIAGQKMVAGHLELAREMVGHALDWDRHGPPAQFLPGNELAEALGIEPGPELGN
                     LMKELAAARFAGEIEDATEAVEHARAFLEAG"
     gene            complement(10975..12120)
                     /gene="alr"
                     /locus_tag="PLJ64_05820"
     CDS             complement(10975..12120)
                     /gene="alr"
                     /locus_tag="PLJ64_05820"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNC93036.1"
                     /translation="MYERAEARIDLAALRHNVRELKSRLDQRVELCPVLKADAYGHGS
                     LHCVGAVAEAGADRIAFATASEAVLARSVAPDIPMLVVGALAPGEIEMAISAGADVAA
                     WEPGFIATLADQAAASGLVVGVHVKYDTGMGRLGATDPELVLELARTADAHPNLRLAA
                     LWTHFATADEKDTTFRDLQRDRLAELGERARREFPEVKLHAANSAAMIADPACHFDMV
                     RPGVAIYGMDPFGNDPADHNLRPVLSLHSWVASVKDFEPGQSAGYGRTWTADRDTRVA
                     AIPIGYGDGVRRGLSNRGEVLIGGRRYPISGTVSMDNITVDLGPDSAVRVGDEVTLIG
                     SQGDERITAEQVAVPLETINYEVTCGISPRVPRVGREGGSPEVSGSR"
     gene            complement(12120..>12453)
                     /locus_tag="PLJ64_05825"
     CDS             complement(12120..>12453)
                     /locus_tag="PLJ64_05825"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNC93037.1"
                     /translation="KVLGIITDSDLVISNEDSDFHLPHYVNIMGGIVFLESTKHWEER
                     AKKAFAATAADMMTADPITVGPDEPAEHAGRLISERKHNRLPVVDDEGRLVGVVTRVD
                     VLAALTGA"
BASE COUNT         2196 a         3950 c         4208 g         2099 t
ORIGIN      
        1 tgatcgcatc gggcatgccg atcaccgcaa tcaacaccgt ccgcaagcat gtgtcgggga
       61 tcaagggcgg gcgtttggcc gagctcatcg ccccggcccg gatcgtgaat ctgacgatct
      121 cggacgtggc cggggatccg caggacgtcc tggccgatct gaccacccag gacacttcaa
      181 cgcccgaaca ggcgctttcg atcctgcgcc gcttcgatct gatcgatgaa gttcccgggt
      241 cgatcatcag ccacctcggc tccccggccg ccctctcgcc cgatcttggc ggaatcgaga
      301 tcagtaccgc gatgctggtc aacggggaga gcgcctgccg ggcgatggcc gacgcagccg
      361 gcgccatcgg cctgaggccg ctgctcgtcg gcaccgcggt cgagggagaa gccgtggaga
      421 cgggaaagac gatcgccagt ctggcgcggg cggagcccgc cggcaccgcc ctgatcggct
      481 gcggcgggga aagcacggtc tccctgggcc gttcggagcc ggggcttggt ggacccaacc
      541 aggaagccgc gatcgccgct tccgccgagc ttgaaggccg tcaggtcgcc gccgtcttcc
      601 tcgacacgga cggctccgat ggcagcaccc gcttcgcggg cgcgatctgc gatggcagaa
      661 ccgccgaacg cgcccgcgaa ctcggcctcg acctcgagtc cgcactggcc tcacacgccg
      721 cctcggagcc gctgagggcc cttgaggacc tgattgacac cggacccacc ggcaccaacg
      781 tcaacgacct cttcgtcgcg gtcacgggcc cgatctgaac cagccgggct gacggagcgg
      841 cgctactcgt cgtcgtcggg gcttccgagg ccgtgcctga ggccgcggaa gatcggcacg
      901 ctaacgatgc ccaggaggat cagcacgagg atcaccgcgg cgaggggcga gaccagcttg
      961 ccgaccgctc ccaccgctac cgcggcgatg atgaccacga tcagccagct gaatgcggca
     1021 ctctcccgct cgaccggatt gccgagatcg aaaccgaacc gctcaccccg ggcgggcacc
     1081 ggcgactgct ccacctcggt ggattcgtcc ccttccccgg cctcgggctc gctcatggcc
     1141 gggtcagatg atgtagacgg cgaggaacac gatgatccac atgatgtcga cgaagtgcca
     1201 gtagatgcct ggcacctcga caccgaggtg gtccttctgt tccggaccga agtggccgcg
     1261 gtaggcgcga atgttggcga aggtcaagag cagcaggcca acgaagacat gggcgccgtg
     1321 gagcccggtg aggctgaaga agatcgaacc gaaggcaccg tcacgcggcg tgaagccgat
     1381 gtggatgtac tcgttgatct ggatgaagag gaaggtcgcg ccgaggagcc aggtcgtggt
     1441 caggccgagc ttcagaccgc gatggttgtt gtgcttgatc gcctcgagcg cgtaatggac
     1501 cgtgaacgag gaggaaacca ggatgcaggt gttgatgccg gcgatcagga ccgggagctc
     1561 gacgccctcc ggcggccagg aagccggatc ggcgacgacc cgcaggaaga agtaggcggc
     1621 gaagaaggcg ccgaacagca tcacctcgga aaggatgaaa agcaggatgc cgaggatcgt
     1681 gcggtcgatg cgggcgctct gatgcgcctc gggcgggccg tgatggtggt cgtcgacgtg
     1741 accgtgggcg gcgatgcttg cgctttccat ctctatgcct cgttctcttc cgcggttgaa
     1801 tcttcagcgc cagcggcttc gggagcttcg ccctcggcga ccacggcagc gacggcctcc
     1861 ggcgggcggc cggcctcgtg gtggaccacg ggcttctcgg tgatctcgcg cacccggccg
     1921 atcaggtctt ccttcagcca gtgggactct tcaccgctca gggtcgaaat caggatctcg
     1981 tcgacccggt aatgctcgat cgcgttcttc acggcgtcga acgggtccgg gctcatcggc
     2041 tggccggtgg cgtcgatgtc ggcgtggtag agcttggcca tgacggtcgc caggttccgg
     2101 gcaacggtgg cctcggaaac atcgtcggcg gccggagcga tgatcgtgta ccggtgggga
     2161 cggtcgccgg agcggtcgag cagacgctcc tgaaggtcct cggaggtcag ggtccggttg
     2221 gcgacgacca gggtgtgggt tacgttcagg ctgatcgagt cgtcctcgat gcgaaccgga
     2281 atgtgggtga tgttggtgac gtcgccgtag gtgatctcgg cccaggagag caggtcgcgc
     2341 ctggtcaggc cgaaacggaa gtcataggtg caggaaagca gaacctcgcc cgggcgatgg
     2401 ctgcgaatcg cgtcgccgag ggccaggtcg tactcggggt cgagcacttc gccgattgcc
     2461 tcgataccaa actcgtcgag caccgcgagg gtcacgtcaa cccgggcccg ggcggcttcc
     2521 gccctttcct cgggatcgaa gagctgaccg accgaaggct ggttctgcgg ggcgacgacc
     2581 accacgtagg aggcgtcggc cgccttttcg cgggccgcct cgagcagctt gcggccgccg
     2641 gcgacctcgt tcaggaaaat caggaggggc ttgctcatga cggcgcagtc accgtgctgg
     2701 gctgggtttc gggcacttcc gccccttcgc cttcgccgcc catgatcgcg tgacgcgcac
     2761 ccggcgtgcc gtactcgtag ggaccgccga cgacccgcgg gatctcgtcg aagttgaaga
     2821 ccggcggcgg agaggagacc tgccactcga tcgagttcgc gcgccacggg ttgttgcccg
     2881 ccttcgggcc gaaccgccag ctgacgatca tgttgtagag gaagatcagc tggaccgcac
     2941 cgagcacgaa ggccgagacg gaaatcagca ggttccagtt gccgtactgc gtcgcgtaat
     3001 cggcgacacg gcggggcatg ccatccatcc cgatccagtg catcgggacg aaggtcatcc
     3061 aggtaccgac cagggtgccc cagaagtgga tccggccgag tcgctcgtca tacatgcgtc
     3121 cggtcatctt cgggaaccag tgatagatgc cggcgaagat ggtgaagacc gagccgccga
     3181 agagcacaaa atggatgtgg gcgacgatga agtaggtgtc cgagacgtgg atgtcgagcg
     3241 ggatcatggc ggtcatcacg cccgagattc cgccgacggt gaaggtgatg atgaaggcca
     3301 gcgagaacag catcgccgtc gaataggtgt ggatcttccc gtagaagagc gtgcccagcc
     3361 aggagaagac cttgatgccg gtcggtaccg cgatcaacat cgtggtgatc atgatcggca
     3421 ctcgcagcca gctgaacatg ccggcgacga acatgtggtg cgcccagacc gagtaggaga
     3481 gcacgacgat gccgatcagg gcgagggcca tgagccggta gccgaagacc ggctttcggg
     3541 catgggtgga aatgatctcg gaaatgatcc cgaagccggg caacatcatg atgtagacgg
     3601 ctgggtgtga gtagaaccag aagatgtgct ggtacgagat gacgtcgccg ccctcgagga
     3661 agttgaagaa gttcatcccg aggatgcggt cgaagaagac catgaactgg gcaccggcga
     3721 cgaacggggt cgcaccgacg accagcagcg aggtcgagag gttggcccag accagcagcg
     3781 gcatgcgcca gaggttcatg ccaggcgccc gcatggtgat gatcgtgacc aggaagttga
     3841 gtgccgtggc gatcgaggag gcacccgcga actgcacgcc gatgttgaag aaggactggc
     3901 ctaacggggc tccggtcgag agcggcgcgt aaccggtcca gccggcgtcg aaggaaccgc
     3961 ccggggcgag gaaactgccg aggaagatga tgccggcgat cggcagcagc cagaaggaaa
     4021 gcgcgttgag gcgcgggaag gccatgtccg gggcgccgat catcagcggc agcacgtagt
     4081 tggcgagccc ggcgaagatc ggaatgatga agaggaagat catcaccacc gcgtgggtgg
     4141 agaagagacc gttgaaggtg ttggcggcga cgatctggcc gcccggctgg gccagttcgg
     4201 cccgcatcag cagggcgagc aggccaccga tcaggaagaa gaacagggtg gtcaccacgt
     4261 actgggtgcc gattaccttg tggtcggtgt tgaaggtgaa gtagtcctgc cagcgggtgg
     4321 cgccgtggcc ggaatgatct tccgggatgg tcggcttgcc tgccgcccag cggaaccagt
     4381 agtcaaaggc accgaagccg aagaggaaac cgaagggcgt ggtgatcagc gggacgatga
     4441 cctgcgggta gccggtctgc tcggtctgga aggcgtcaag gcccgaaaga aggcgcagtc
     4501 cgaccaccag aaagaacccg aacgcggcac cgagaacggt tccgatcgct gcgcgatacc
     4561 aaccgggttg cctgattgcc gctgccattt acttgacctc catcatcggg ttgtggaacc
     4621 gggccttcac tgttctttga ccccgcttcc ttcaatgccc ccggactgct cctgctgctg
     4681 tttctgcttc tccagcgcct gatcggtggt caggagaaga tcgtcaggca cttcggtggt
     4741 ctgctgcttg agccaggcct ggtacttcgc ttcggactcg acgaccacga tcgcacgcat
     4801 cgtcgtgtgg ccgatgccgc agagctcggc acagatcaac tggtaggtgc cttccttgtc
     4861 cggcgtaatg atcgcgtcgg tttcaagtcc cggcaccgcg tccttcttga tccgccattc
     4921 cggaacccag aaggagtgca gaacgtcctg cgcctgaagg tcgatgatga tctgccggtc
     4981 gaccggaacg tgaagctcgc cctccgacca tttgttgccg ctctccgggt aaccgaaggt
     5041 ccaggcgaac tgctgggcat aggcgttgat gtggatcgcg tccgcatcgg tcttctcgat
     5101 gtcgtcgaga acggcccacg agtagccggc caggccgagc accatgatga tcgggatgat
     5161 cgtccagacg atctcgagct tcgtgttgcc gtggatcggc agtccgtcgc tttcgtcacc
     5221 gggcttggcc cgccacttga tcagggcgta gccgagggcg acgcagacga tcgcgaagac
     5281 aaacgacgag acgacgatgg tgacgtccag cagcgtgtcg atcggaccgg ccgcggtgga
     5341 agctgcatcg ccgaaccagt cgacctggag cagaaccaca gactggacca cgccgaggac
     5401 gattacggcc agcaccattt tcaaaatcgg gagacgagtc atgaggcgac cgccattcta
     5461 ttagagccgg ggacgaatgc tttctgaccc tgcgctcagg actggacgac cttccgcact
     5521 tggcctgctg atgcggaaat cacatccttt gcgaccccct gccagctcca attccgatcg
     5581 gcggactcgg caatgttttg accgatctca agccgctccg ccgggtcgag ggccagccag
     5641 cggttgacct tgccggccag ctcggccacg gctcccggcc cgagtggcag cccgatcatc
     5701 gattccacgg cgggaacttc ctcggccagc gtggcggtga cctcggccag gcccgagtgc
     5761 tcggcgcaga ccggaagcac cccggaagcg gccgcttcgg cggcgaccat gccgaatgcc
     5821 tcggggaagg tcgagggcac gatcatcgag tcggcggccg gcacaaccct ggccacctca
     5881 tcgtgatcca gccggcccgc gaaggcgatt gacccggccg cctggacacc ggcctcctcg
     5941 tatccggccg gcaggtccga gaagaagtcg gtcaggtagg aaaggggttc ctcctccccg
     6001 ccttcgagcc cgcggccgat cgcggcgatg tcgcgggccg actggatgtc cccgtcggtc
     6061 aacgcggtaa ccagggcccg caaacccatc tcgtaggcgc cgaagccgac cagcatcagc
     6121 ctcgccccgg ggttctggcg gtggatcagc ggccaggcgg cgacgatcag gtcgacaccc
     6181 ttggagacga tcagcttgcc gacgaagatc acccgcggcc cttccgcggc cgcgaacgcg
     6241 tccagagcag caaccgcttc gtcggcgtcc cgcccgaacg tggttgacgg cggctcgttt
     6301 gcgatctcgt tcctgagagc cgcgatccgg ccctcatgct cgcccggctt cagcggagcg
     6361 aacgcctgcg tgtcgacgcc gggcgggccg aagccggtct tttcctcgag gccgagaccg
     6421 ggcagcgcct cccacatcga ggccgccgtg tggtgcgaac cgaccaggac cgccgctgcg
     6481 gattccactc cttcggccgc ccaggggatg aatcgggggt tgggcttgat cgtgtactcg
     6541 aggtcggatc cgtggttctt gaccgcgtat ggccgcaccc cggcccgggc gaggatcacc
     6601 ggacccatga tcaggtgatt ggccagggcg gcgtcggatc ccccgatctg ccgctccacc
     6661 gcctccacgg cggccacgtt cgatcccacg tagaagtcga tctcctgatc ggtcagccgg
     6721 gggaaggccc gggcctcgaa cccctcgtac ggatcctcga cataaacggg cagcagtttc
     6781 ccgatcggcg gccggtagac cgtcaccgac ccctcgcccc cgggctggcg gatctgttcg
     6841 aactgaagct cgccgatcga ttccggccct tccgcgtccg gccaggtgcc gaccgcattc
     6901 acccagtcga ggctcccggc ctcgcggtcc tgacagagca ggtggacttc gtggccgagg
     6961 ccggcgagag cccgggccag gttggcgttg tagatgttgc ttccggtgcc gcggagcagg
     7021 tagccgtgaa agatgaggat cttcatgtcc ggctattctc caccttgatg agcaccgaaa
     7081 gcgtctccaa cgaacagatc taccgacttc gtgccgcggc ttccggtcgc gaggccatcg
     7141 cccccgtcaa ggacggcgtc gtctaccggg acgcggaaac cggcgaggaa ctcgaacccg
     7201 tcgccgccgt gctgccgaat accggagagg gctccagcct cccccgcacc ccggtgaacc
     7261 ttcgcgtctg ccgccgctgc gagcagctaa ccccccgcga tctcgacacc tgcgagttct
     7321 gcggcctccc cgcttcctga tttcacgagt ttcatccggg tgcttgtact tagttggcca
     7381 ctgaccaact aagtacaagc acccggatcc tcagtcttag ggagcgacga cagtcgcctt
     7441 gtagagcagg cggccggcgt cgtaatagca ggcgtatcgc ttgatcgaat tgaccttctg
     7501 ctggggatgc gaattcctca gggcttcatt cggctgatcg aaggcattgg tgccaatcgc
     7561 gcattggccg ggtccgcggc tgccccgcca ggtgttggcc ttgcgcgcgt cggcgcagcg
     7621 ggccgcattc gcagtgcccc gatcggaagg actgcatgcc atcggaaccc agaaggccgg
     7681 cgcccaggtc gggatcgtga gggcgacgac gtcaccgcgg atcaccttga gcggcttgat
     7741 cgcgaacttg atctcgctgc cgaagtagcg attcagcttc tgggtgccgc tggccctgat
     7801 caacttgtag cggggcgggt acctggcgcc ctttttgatc ggccggagga tcgagatccg
     7861 tgccttcgag ggtgagccaa agagcttgtt gaagtaaggc tgctgggcag gcccgaaccg
     7921 gcggctgtct tccttgagcg gattcgaaag ctggatcgac caggccacga ctttgccctt
     7981 cttgaaggga accacgaagt tcttgccggt gacccccttc tggaagacct ggaaaccggt
     8041 caccttgcct tccgcgatgc aggcccgtgc gccggacctg gtaccgcagt tcggggtgac
     8101 cttgtccctg gttgccccga gcacgacccg ccggttgctc ttgccatcgg catcgccgaa
     8161 agcgccggca aggacgaggc aggcgaagag acccagagtg gctgcaacga gcgcgccgag
     8221 gcgaagggcc gggcggctca cgaaatgtga acgaggttcg gccatacagg atccaacacc
     8281 gaggctacgc cacaggtgcg gaacttcaag tcagagcggc ggtcgggaaa tcctgcccgg
     8341 acggtccaac ggctcgtagc cggggaaata tccgaccggg gtcagcggcc gacgatgatc
     8401 gccgagtaga gcaggacgtt gccgccgtag tagcagccgt aacggcgaac cgagtcgaga
     8461 tgggtctgcg gagcggtctt ctggagctgc tcgttcgggc cgccgtcgtt gctcagtccg
     8521 caggtttcgg gttcaccccg ggccacgcga ctgccacgcc aggtgtaggc cttctcggcc
     8581 tgatcgcaac gaccggggtc gagaatgccg aaatcggtct ggttgcaggc cttcggcttc
     8641 cagagcgccg gcgcccaggt cgggatcgtg agtgcgacga cgttgccttc gtagacgttg
     8701 agcggaacgt cgagcgcgaa ggtgaccttg gtcccgaagt agggattgag gatctggatc
     8761 gggctcttgc gaaccagctt gaagcgcggg ccacccttct tgcgcttctc gacctgcctg
     8821 aggattgcga tattggcctg tgccggtgaa ccgaagatca ggttgaagga gggaagctgg
     8881 gctccgttgt tgtcgatctc gacccgggtc gggttggaaa gtgaaatcga ccaggcgacc
     8941 agcttgccgt tgaacggcac cacgaagttg cgccccggat accgggcgga aagtgactgg
     9001 aaggcagtca ccttgccttc gaccgtgcag tcgcgagtga agtcccggcc gcagttcggc
     9061 tgaagggtgt tgatgttgcg ggcgccgagt tcgatccgct gcgtggactt ggcctccgca
     9121 tgcggtgcga aggcgatcag gatcagggcg gcaaggccgc tcaggaccgc gaaaacccgg
     9181 gcgacccgaa ggccgtcagt tgtgaggggg gagcgaagca tcgaatactg gaacacggtt
     9241 gggtgcctca atgttgcgct ccgggatctg atcggacccc ggtcgtggcg atcgttactt
     9301 gtagcggtag gtgatgcggc cgcgggtcag gtcatagggc gaaagctcga tcttgacccg
     9361 gtcccccggc aggatgcgga tgtagtggcg acgcatcttg cccgagatgt gaccaaggac
     9421 ctcatgatcg ttgtcgagct tgaccctgaa catcgtattc gggagggctt cggtgatttc
     9481 accctccatt tcgatctttt cttctttagc catgcggaga tgaggctact actcacccgg
     9541 cttcgaggaa ggcacgggca tgctctacag cttcggttgc atcctcgatt tcaccggcaa
     9601 aacgggcggc agccagttct ttcatgagat ttccgagctc cgggcccggc tcaatgccca
     9661 gagcctcggc cagctcgttg ccgggcagaa actgtgccgg cgggccatgt ctgtcccagt
     9721 cgagggcgtg gccgaccatc tcccgggcca gttcgaggtg acccgcgacc atcttctggc
     9781 cggcgatctc ggccgtgccc cgggcagcca ggcggtcggc caccgtgagc aaagtcacat
     9841 cgatcgagac cggatcggtg tggtcgaggt aggcgaagac ctggcgtttg tcgagcggca
     9901 tctggttgac caggaacccg agaatcagat gatgccgggt caggtcggca atgtaggagg
     9961 acagtttccg gctggccctc aggctgcgaa accggctgtc gatctgttcg gccccgagcc
    10021 ggtcgtggcc acggaaggag acgaagccgc cctcctcgct tcgggtttgc ggtttggcgc
    10081 aatcgtggaa gagcgccccg agccggagcg ccgcgccgcg ggtagcgccg tcggccagcg
    10141 gccgggagag gtaagcggaa acctcgggtg cccgctcgcc ggtgaaggtc tcgaggtcgg
    10201 attcgatcct gaggatccct tcgaccactt ccatggtgtg gccgtagacg tcgagatggt
    10261 ggttcggacc ctggaccaca tcgtggaggt caccgatttc cggcagcagc cagtcaaaga
    10321 ggccgatccg gtccatcgca gccagcccgg ccagcggatc gcgggccccg atcaggagga
    10381 tcaactcggc cagggtgcgc tcaccggccg ggtccccggc ccggggggcg gattgccgtg
    10441 ccagctctgc ggtcgcctcc tcgatctccc agccgaactg ggcgaccagc cgggccgccc
    10501 gcatcagccg cagcgggtcg tcgctgaacg atgtcgggcc gcagaccctg atcgtgccgg
    10561 cagcgagatc gccaagtccg ccgagcgggt cgatcgcctc gccgttttcc aggcttacgg
    10621 ccaccgcgcc gaccgtgaag tcgcgcagtt ccagatcccg ctcgatgtcc ggggccctga
    10681 gttcggccac gtccacctgc caggcgtcgt cgcgatcctt ggcccgccag gcgggaaact
    10741 cctccgaaag ctcgaaggcg attccgtcca gagccttggc aatggcccgc gccgggacct
    10801 ccggtgaccc ggtcaccgcc aggtcgagat cgttggtctc ccggccgagt gcgatgtccc
    10861 gcaccgcgcc gccgaccagc caggcggggg taccggccag ggcttcgcga acccggtcga
    10921 cccggggcgc gcccagcgcc cgctggccga gaacttccag accgaggacc ctggtcaccg
    10981 ggaaccgctg acttccgggc tcccgccttc cctcccgacc cggggcaccc gcggcgagat
    11041 cccgcaggtc acctcgtagt tgatcgtttc aagcggaacc gccacctgtt cggcggtgat
    11101 ccgttcatcg ccctggctgc cgatcagggt cacctcgtca ccgaccctga ccgccgagtc
    11161 ggggccgaga tcaacggtga tgttgtccat cgagacggtg ccggagatcg ggtaacggcg
    11221 gccgccgatc aggacctcgc cccggttcga aaggccgcgc cgcaccccgt cgccgtagcc
    11281 gatcgggatc gcggccaccc gggtgtcacg gtcggcggtc caggtccggc cgtagccggc
    11341 gctctggccc ggctcgaagt ccttgaccga ggcgacccag gaatggagcg agagcactgg
    11401 ccgcaggttg tgatcggccg ggtcgttgcc gaacgggtcc atgccgtaaa tcgcgacccc
    11461 gggccggacc atgtcgaagt ggcaggcggg gtcggcgatc atcgcggcgc tgtttgccgc
    11521 atggagtttc acctccggga attcccggcg tgcccgctcc cccagttcgg ccaggcggtc
    11581 ccgctgcagg tcacgaaagg tcgtgtcttt ttcgtcggcc gtggcgaaat gcgtccagag
    11641 cgcggccagc ctgaggttcg gatgcgcgtc ggccgttcgg gccagttcga gcacgagttc
    11701 cgggtcggtg gcgccgagcc ggcccatgcc ggtgtcgtac ttcacgtgaa ccccgacgac
    11761 cagcccggaa gccgccgcct gatcggccag ggtggcgatg aagcccggct cccaggcggc
    11821 gacgtcggcg ccggccgaga tcgccatctc gatttcaccc ggggcgaggg cgccgaccac
    11881 cagcatcggg atgtccggtg cgacgctgcg ggccagcacc gcctcggacg cggtggcgaa
    11941 ggcaatccgg tcggccccgg cctcggccac ggcaccgaca cagtgaagcg agccgtgccc
    12001 gtaggcatcg gccttcagga ccggacaaag ctccaccctc tgatcgaggc gtgacttcag
    12061 ctcccggacg ttgtgccgca gggccgcgag gtcgatccgg gcttccgccc gctcgtacat
    12121 caggccccgg tgagggcggc caggacgtct acccgggtga ccacgccgac cagtcggccc
    12181 tcgtcatcga ctaccggcag gcggttgtgc ttcctctcgg aaatcaggcg accggcgtgc
    12241 tcggccggtt catccgggcc gaccgtgatc gggtcggcgg tcatcatgtc tgcggccgtc
    12301 gcggcaaatg ccttcttcgc ccgctcttcc cagtgcttgg tcgattccag gaagacgatg
    12361 ccgcccatga tgttcacgta atgcggcagg tgaaaatccg aatcctcgtt cgagatgacg
    12421 aggtcggagt cggtgatgat cccgagcacc ttc
//
LOCUS       DAOQKN010000063        12295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_2411_length_12295_cov_5.880637, whole genome shotgun sequence.
ACCESSION   DAOQKN010000063 DAOQKN010000000
VERSION     DAOQKN010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 12295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12295
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2411_length_12295_cov_5.880637"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1339)
                     /locus_tag="PLJ64_05830"
     CDS             complement(<1..1339)
                     /locus_tag="PLJ64_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC93038.1"
                     /translation="MAALAEVSLAIEPGEFIVLAGGSGSGKTSLLRAGCGLIPHFHGG
                     EIAGAARVGGRSILESGPGELADVVGYVAQDPETQIVSTTVAAELDLPLRFRGDHPAE
                     RARAVEEVALALGIPHLLPRTVTTLSGGELQRVALAVALVTRPPLILLDEPTSQLDPV
                     SGDELIWLLRRLNEEWGVAVVLAEHRLERCLPAADRVVAMDEGRIGFDGTPRDFLAWA
                     IANKPELATPSALMFAGSDIEPLPVGVRDARQILRARDSGGDQPPNRWPDAPGKRGRG
                     LDSGSRAGSSVALEAKRLWVELDPGDGPVDVLRGIDLEVQAGERVALMGRNGAGKSTL
                     LRAAAGLIDPVSGRIRAPGGIALLTQNPGDFLVRERVGDELSGPAGEAALGAVGLDVS
                     PDRDPRDLSGGERQRLALAIALAGRMEGEAVPGLVCLDEPTRGMDRGLKGHLTE"
     gene            complement(1429..2319)
                     /locus_tag="PLJ64_05835"
     CDS             complement(1429..2319)
                     /locus_tag="PLJ64_05835"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HNC93039.1"
                     /translation="MKKLSPIAFVPRRETVQSASLAPVALYLGAYLLVCFFSSDPLVL
                     VFATAGAALAGYGCGAGRAVNFSLKLGAFLALTMVIVNALVTSRGATVLARLGDWPLL
                     GQVEVTAEAIAAGMLIGLRVIGTMVVIGVWSACVDPDRILQAVHGIARRSALTATLIS
                     RLVPLAATDYARIGEAAELRGPAASPVSRGAMVHRLLAGSLDRAVDVAATLELRGYGL
                     SAESTRFRPAPSRFDRRFWIGGLLLTLLAFFELLAGSGRFVTYPGISYPVGAEGPLAA
                     LTFLLCGFVTWRRGGRGVRR"
     gene            complement(2328..3296)
                     /locus_tag="PLJ64_05840"
     CDS             complement(2328..3296)
                     /locus_tag="PLJ64_05840"
                     /inference="COORDINATES: protein motif:HMM:NF025833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HNC93040.1"
                     /translation="MKSATESRRSGGFPARAVGMAVALFFGLLLAGCGLGPGEDTGDA
                     SLLVTRDYGSRVLFDNPGLAVNESSNAMRLLDGNAELETRYGGEYVQSVDGLSGDRKD
                     GRSFDWFFAVNGIVAERGSAQFPVTGGDRVWWDYRDWTDAMEVGAVVGSYPAPFSTGY
                     DNRDWGVEIDCLGEETACRMVENQLEGDGVTLKDRGENMRIVVGALPDLLEIPEMKRL
                     NRGPSASGVFAGFGVGPVLEKDGKPAPTLAVEGLRVDGRVGRKYGPDAGLVAALRRAD
                     DPPVWLVTGGTDQAVRMAAAALNPGDLARRYAAVVNDGQVSSLPLP"
     gene            complement(3306..4031)
                     /locus_tag="PLJ64_05845"
     CDS             complement(3306..4031)
                     /locus_tag="PLJ64_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93041.1"
                     /translation="MTNRITSPALGLLLGLTLLLGLAAQPAAAGNRVAANLRVVTWKG
                     QIIFDGKVKTGTAKIKPNDDCLGGHAGPARTVTGPTALGLLYQASLRYKALQPLKISD
                     GDFGFGICGIADVSAKNKEWWVLRRNNKDSMSGAETTRVKAGDTILLYLARTYEDTAP
                     DSLVLNAPTKARKGARVKVRVFSFTGAGKRTPVEGARVFGAKGALTDAQGYTYLTING
                     RTNLVARQGSLIPSNRVSVSIRK"
     regulatory      complement(4079..4262)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            4347..5042
                     /locus_tag="PLJ64_05850"
     CDS             4347..5042
                     /locus_tag="PLJ64_05850"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNC93042.1"
                     /translation="MPVAVGPSWLDPESLIRTFGMLGVLAIVYMESGLMVGFFLPGDS
                     LLFTAGLLSANDVLPDIWVLLVTIPIAAIAGDQTGYWIGRKFGPPLFNRPDSRFFKRE
                     YVDQTAAFFEKHGPRAIILARFVPIVRAFVPVMAGTSHMHYRTFLVYDIVGGILWGAG
                     VTTLGYFLGQIEFVKNNIEFILIGVVAVSVIPVIFEVRKARREAASHQKPEPFENPID
                     EEGEPVKHAESEV"
     gene            5105..6709
                     /locus_tag="PLJ64_05855"
     CDS             5105..6709
                     /locus_tag="PLJ64_05855"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93043.1"
                     /translation="MREIKRCLAVVLALGAVFSVPAASAHQIPAGCLGPKPTVTFSAD
                     TLSQTAVPLRDGDQLTLAAHVDNKGESACNLSGLSIMVRPPSADEVSSDHFLLIDNVP
                     LGAGYDAEIINHGPYVVDLADDVFESQLELSWQATIHNGQADETVTGTGPGTKLTVTR
                     PQVELKVIPDVQSGPAPLTVTTVYLLTNTSPVPTAGTPAPGLVPTGSNGPRDVLADAG
                     CGPLNYLSGDLPADTDPVLDPGETWIFTCLRTYPLPGTYASQPVITGLSTADGRAWPE
                     QPLGAEPPSVTALGPDLVVAKSHQGDLLAGSSGSYELEVTNTGNQPTDGAVTVADQLP
                     AGLTGTAIAGDGWTCTLASLSCSRSDPLGSGNSYPEVTVTVAVSDDPPDNLVNTATVS
                     GGGEPAGAVANNLDEDPTTVRIPSQPTPPSNRIFKVRKVTSRGDGSALIKVAVPGAGV
                     VAADDAGKSDLVQRAWQKSSKARTVSLVVKAGSKLRKALVASGKPRQVRVRISFTAKG
                     SPKATPTTSTVRSISFLISPPQTLAN"
     gene            complement(6706..8097)
                     /locus_tag="PLJ64_05860"
     CDS             complement(6706..8097)
                     /locus_tag="PLJ64_05860"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC93044.1"
                     /translation="MTRRDLGKAALAGAAVAGLGGAAGLAGCAREGGGNGSGASKQEV
                     IDGLPETGIPRPVDYIRTDWSHDPLAFCSYSCLAPTPYGSEIRRMLAEPVGRIAFAGE
                     ATARIPATVHGAIQAGRDAGKYAAGQLRAGARVAVVGAGAAGLSCAGELERLGLKPVV
                     LEASDRVGGRVREADLDGSPVELGASWIHGVDGNPLTELAGEAGVGLHPFRYQLDFPV
                     PGQQALGAGGEHRYWQAVNSFGPRSVRHPDVPLEAILPRRRGPGLEWAIQDEVAQEYG
                     ADPDQLAALATWEGNWLPGGDALLDGPYSQVIGSLSGDAGIRFGFEVRKISQRPGEVR
                     LTGAEDNDEVAADAAVVTVPIGVLKAERIEFDPPLPARTRTAIDGLGAGLLDKVWLAF
                     EEPFWDENAEGFSWIDPGRPGRWGAWVNAVPVTGEPFLLTLSGGRDAHRLSRQSDLEI
                     ISGTTAALNSMFG"
     gene            complement(8185..8257)
                     /locus_tag="PLJ64_05865"
     tRNA            complement(8185..8257)
                     /locus_tag="PLJ64_05865"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8221..8223),aa:Glu,seq:ctc)
     gene            8396..8479
                     /locus_tag="PLJ64_05870"
     tRNA            8396..8479
                     /locus_tag="PLJ64_05870"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8430..8432,aa:Leu,seq:cag)
     gene            complement(8515..10638)
                     /locus_tag="PLJ64_05875"
     CDS             complement(8515..10638)
                     /locus_tag="PLJ64_05875"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HNC93045.1"
                     /translation="MWQGRGWQGLATVIAIAISILVPATANAGEGGFTTGRLGPYQIR
                     FSDSPFGFSISRGGRYLLAGLGHSFERNGVRYATLGYTPQPAKELEPPDLSAAPDEEP
                     STQTSYVATRANGMWFGPDVYEARPRTDSPGGRRMRLTIWLRKDGTLGIRAGMMAGKA
                     GSVYLGFRSPQSEAFHGFGGRREGTDLRGSDIKSWVLDYRYPDPTTAYYSPVPGFISS
                     RGYGLLLKGDRISRWRMASDRPTAWRVSRPGRSMEMTLTSGGQARAMAGISRLTGRHR
                     LPPAWATGTVLSRAIGIAADSGGKYQSRVESDLEHLASADFPISAYAFEGWQALPGSF
                     VKATIRTLKEEGIHPVLYLRSFVSNDTAGTEGPGTFDQAISRKLVATGKDGKPFLLPS
                     PFPGGQAAVIDLTKPAAVRWWRNRIWNLLDLGADGFMNDFGEQVEAGMRFHDGTSAAL
                     MHNRYPALQAKVTRAAVDAWERRHPKRHIFFFQRAGFTGSLSSVKWESAQFPGDETVD
                     WGSDTGLPSIVPDMLNRALTGAVGFTTDIGGYSQFRTEQPFIPPTGEELFTRWAQAAV
                     FTPFFRVHNSGLSGARMPWDFSTDAQSTWKKMVVLHNRSRPLIRRLWKRFLKTGMPMI
                     RPLYLAGGVGPKNPHNDDEWMVGHDLLVAPVLEEGAVTREVLLPSGCWRRSGTGPPVE
                     GARTVEVGAALDELPWFTRCGTSPL"
     gene            10676..11491
                     /locus_tag="PLJ64_05880"
     CDS             10676..11491
                     /locus_tag="PLJ64_05880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNC93046.1"
                     /translation="MKIFSWNVNGIRAVIRKGDFGSFVEKYDPDILCMQETKAEQGQA
                     EIDLPQYEEYWNSAGKKGYSGTAIFTRQKPIAVLNDFAAEISDEYDLHNDSYGDPRAE
                     GRVITAEYDHFFLVTTYVPNAKRDLSRLKLREKQWDPALLHHMKMLDAKKPVLVCGDM
                     NVAHTEDDLANPKANAGEHGFTDEERQGFQNFLDAGFLDTFRLFAEGNGHYSWWSYMS
                     KARERNAGWRIDYFLASERLRQAITGAEILGDVMGSDHCPVSVELDEKKLAKL"
     gene            complement(11558..>12295)
                     /locus_tag="PLJ64_05885"
     CDS             complement(11558..>12295)
                     /locus_tag="PLJ64_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93047.1"
                     /translation="AAIYLAKVNLPTALITDPILIAFNAGYTGSGQPRIKIYGYSKTT
                     NVGILMNGTLKGSVLSMAVPVLSNDSAVKYFDLNFPGGRLDREDIGVHVRGLDPNYVQ
                     ARCASSPLKTNAVFDLGERTYPAGTDVGQTTRVASPETMQSCVGLAGHAKLKAKVVKK
                     PRAVRNGGRGFFRVYVRNYGTATAKGVYLFAPSVKKKVGWIAPGVARTIGIRARVRGR
                     KGHSRTIRFTVKGNGVSASAFARVRVK"
BASE COUNT         2118 a         4243 c         3936 g         1998 t
ORIGIN      
        1 gttcggtcaa gtgtcccttg agcccccggt ccattccgcg ggtcggctcg tcgaggcaga
       61 ccaggccggg caccgcctca ccttccatgc gtccggccag ggcgatcgcc agggccagcc
      121 gctgacgctc gccaccggaa agatcgcgcg gatcccggtc gggtgagaca tccagaccga
      181 ccgcgcccag ggccgcctcc ccggccggac ctgaaagttc gtcgccaacc cgctcgcgga
      241 ccaggaaatc acccgggttc tgggtcagaa gcgcgatccc ccccggtgct ctgatcctgc
      301 ccgagaccgg gtcgatcaag ccggcagcgg cccgcagcaa agtcgacttg ccggccccgt
      361 tccggcccat cagggccacc ctctccccgg cctgtacttc caggtcgatt ccgcgcagca
      421 cgtcgaccgg gccgtcaccg gggtcgagct cgacccagag tcgcttcgct tccagtgcca
      481 ccgaagaacc ggcccgtgaa cccgaatcca gacccctccc acgctttccg ggggcgtctg
      541 gccaccgatt cggtggctga tcgcccccgg aatcgcgggc gcggaggatc tggcgggcat
      601 cgcgaactcc gaccggcagg ggctcgatgt ctgacccggc gaacatcagg gcagaaggcg
      661 tggccagctc gggcttgttc gcgatcgccc aggcgaggaa gtcccgggga gttccgtcga
      721 agccgatgcg gccctcgtcc atggcgacaa cccggtcggc ggcgggcagg cagcgctcga
      781 gccggtgctc ggccagaacc accgccacgc cccactcctc gttcaggcgc ctgagcagcc
      841 agatcagctc atcgccggat accgggtcga gctgcgaggt cggttcatcg agcaggatca
      901 gcggcggccg ggtgaccagg gcgacggcaa gcgcgacccg ctggagctca ccgccggaga
      961 gcgtggtcac ggtgcgaggc agaaggtgcg ggatgccgag cgcgagggca acctcttcaa
     1021 ccgcccgggc ccgttcggcg ggatggtcgc cgcggaagcg aagcggcagg tcgagctcgg
     1081 ccgcgaccgt ggtcgaaacg atctgggtct ccgggtcctg ggcgacgtat ccgaccacgt
     1141 cggccagctc accaggcccg ctctccagga tcgagcggcc gccgacccgg gcggcaccgg
     1201 cgatctcgcc gccgtggaaa tgcgggatca atccgcaacc cgcccgaagc agcgaggtct
     1261 tgcccgatcc cgaaccaccg gccaggacga tgaactcgcc cggctcgatc gccagcgaaa
     1321 cctccgcgag ggcagccacc tccgacccgg agtatcggta tccgagccct tcgaattcga
     1381 gcgccagacc agccctgcgg ccggctgacc gttcggcggt aaccgggttc accggcgcac
     1441 ccccctgcca ccccgccgcc aggtcacgaa tccgcaaagc aggaaggtga gggcggcgag
     1501 cggcccttcg gccccgaccg ggtagctgat cccggggtag gtgacgaaac gaccggatcc
     1561 ggccaggagc tcgaagaagg cgagcagggt caggagcaaa ccgccgatcc agaagcggcg
     1621 atcgaaacgc gaaggggccg gcctgaaccg ggtggactca gccgatagcc cgtaaccgcg
     1681 aagctcgagc gtggctgcca cgtcaactgc ccggtcgagt gaaccggcca gcagccggtg
     1741 caccatcgcc ccccggctga ccgggctggc cgccgggccg cgaagctccg ccgcctcccc
     1801 gatccgggcg taatcggtgg ccgcgagggg aaccaggcgc gagataaggg ttgcggtgag
     1861 agcggagcgg cgggcgatgc cgtggaccgc ctgcaggatc cggtccgggt cgacgcaggc
     1921 cgaccagacg ccgatcacga ccatcgtgcc gatcacccta agaccgatca gcatgcccgc
     1981 agcgatcgcc tccgcggtta cttcgacctg ccccagcagg ggccagtcac cgagccgcgc
     2041 gagaacggtg gctccgcggc tggtgaccag cgcgttgacg atgaccatgg tcagagcgag
     2101 gaaggcaccg agcttcagcg agaagttcac cgcccggccg gctccgcatc cgtaaccggc
     2161 gagcgccgcg ccggccgtgg cgaaaaccag taccaggggg tcggaagaaa agaagcagac
     2221 cagcaggtag gcgccgaggt agagcgcaac cggcgcgagc gaggccgact gaacggtttc
     2281 ccgccggggc acgaaggcga tcggggagag cttcttcatg gccggcctca gggtaagggc
     2341 agggacgaga cctgtccgtc gtttacaacc gccgcgtacc tgcgggcaag gtcgcccggg
     2401 ttgagcgccg cggccgccat ccgaaccgcc tggtccgttc cgccggtgac cagccagacg
     2461 ggcggatcgt cggctcggcg caatgcggcc acgaggccgg catcggggcc gtacttcctt
     2521 cccacgcggc catcgaccct tagtccttcg actgcaaggg tcggagccgg tttgccatct
     2581 ttctcgagaa cgggtccgac gccgaaaccg gcgaagactc cgctggcgga gggtccccgg
     2641 ttgagtcttt tcatttccgg gatctcgagc agatcgggaa gagcgccgac cacaatccgc
     2701 atgttctctc ccctgtcttt cagcgtgaca ccgtcccctt cgagctggtt ctcgaccatc
     2761 cggcaggccg tttcctcacc gaggcagtcg atctcgacgc cccagtcccg gttgtcgtag
     2821 ccggtcgaaa agggggccgg gtaggaaccg accacagccc cgacctccat cgcgtcggtc
     2881 cagtcgcggt aatcccacca gacgcggtcg ccgccggtga ccgggaactg ggccgagccg
     2941 cgttcggcaa cgatgccgtt aacggcgaag aaccagtcga aactgcggcc gtccttccgg
     3001 tcgccggaga gcccgtcgac cgactgaacg tactcgccgc cgtagcgggt ctcgagttcg
     3061 gcgttgccgt caaggagccg cattgcgttg ctcgactcgt tcaccgccag accgggattg
     3121 tcgaacagca cccgggaccc gtagtcgcgg gtgaccagca ggctggcgtc acccgtgtcc
     3181 tcgcctggcc cgagcccgca gccggccagg agcagaccaa aaaacagtgc aaccgccatg
     3241 ccgacggccc gggcaggaaa gcccccggac cgtcggcttt cggttgcgga cttcactcgt
     3301 tccgcttact tccggatcga aaccgagacg cggttcgacg ggatcagact gccctggcgg
     3361 gcgaccagat tcgtcctgcc gttgatggtc aggtaggtgt atccctgcgc gtcggtcaac
     3421 gctccctttg cgccgaagac cctggccccc tcgaccgggg tccgcttgcc ggcaccggtg
     3481 aaggagaaga cccggacctt gaccctggcc cctttccttg ccttggtggg agcgttcagc
     3541 accagtgagt ccggggccgt gtcctcgtag gtcctggcga ggtagagcag gatcgtgtcg
     3601 ccggccttga ccctggtggt ctcggcaccg gacatcgagt ccttgttgtt ccggcggaga
     3661 acccaccact ccttgttttt ggccgagaca tcagcgatac cgcagatgcc gaagccgaag
     3721 tcaccgtccg agatcttcag cggctgaagc gccttgtagc ggagcgatgc ctggtagagc
     3781 aggccaagcg cggtcggacc ggtcacggtg cgagccgggc cggcatggcc gccgaggcaa
     3841 tcgtcgttcg gcttgatctt cgcggtgccg gtcttcacct tgccgtcgaa gatgatctga
     3901 cccttccagg taactacccg gaggttggcc gcgaccctgt ttccagccgc ggcaggctgc
     3961 gcagccaggc caagcagcag ggtcagcccg agcagcaacc cgagggcagg tgaggtaatt
     4021 cgattggtca tgcgttcctt ttgtccggcg gccctacgcg agaccgcttt ttcggtgcgc
     4081 catgtggcgc aaagccggca ggtcttcgga cttggacgtt tgctgggagg cagccttccc
     4141 agaccaatgg tccagtggct ttgtgccttc aaggacgtcc ctaccgctgc gcgtcagttc
     4201 cggactcgga ccggattccc tgaccccgct gaaggggtgc gaccgacttc gcaggcgatg
     4261 atagagactc ctgacgcggc gtgacctgtc aagatgccgg attccgcccc tcgcccatgg
     4321 actttctttc tcatgtcata gacaggttgc cggttgccgt cgggccgagc tggctcgacc
     4381 cggaatcgct gatccgcacc ttcggcatgc tcggcgtgct ggcgatcgtc tacatggaat
     4441 ccgggctgat ggtcggcttc ttcttgcccg gcgattccct gctgttcacg gcgggcctgc
     4501 tctccgccaa cgacgtgctc ccggacatct gggtgctcct ggtcaccatt ccgatcgccg
     4561 cgatcgccgg tgaccagacc ggctactgga tcggccgcaa gttcggaccg ccgctcttca
     4621 accggcccga cagccgcttc ttcaagcgcg agtacgtcga ccagaccgcg gcattcttcg
     4681 aaaagcacgg cccgcgggcg atcatcctcg cccggttcgt cccgatcgtc cgggccttcg
     4741 tgccggtgat ggccggcacc tcccacatgc attaccgcac cttcctggtc tacgacatcg
     4801 tcggcggcat cctctggggc gccggcgtga ccaccctggg ctatttcctc ggccagatcg
     4861 agttcgtcaa gaacaacatt gaattcatcc tgatcggcgt ggtcgccgtt tcggtgatcc
     4921 cggtcatctt cgaagtccgc aaggccaggc gcgaagccgc atcccaccag aagccggaac
     4981 cgttcgagaa cccgatcgat gaagaaggcg aaccggtgaa gcacgccgag tccgaagtct
     5041 gaaccccgtt ttcagccggc cgcggtcagg gtcggtactc ttcaggcaga aggcaagggg
     5101 ttcgatgagg gagataaagc gatgtctggc ggtcgttctg gcactcggcg cagtgttttc
     5161 cgtccctgcg gcaagcgccc accagattcc cgcaggctgt ctcggcccca agccaaccgt
     5221 caccttctcg gctgacaccc tcagccagac ggcagtgcca ctcagggatg gcgatcagct
     5281 caccctcgcg gcccacgtcg acaacaaagg tgagtcagcc tgcaacctga gcggcctctc
     5341 gatcatggtc cggccgccaa gcgccgacga ggtctcgagc gatcacttcc tgctgatcga
     5401 caacgtgccc ctgggtgcag gttatgacgc ggagatcatc aaccacggcc cctatgtcgt
     5461 cgatctcgcc gacgacgtct tcgaatcgca gctcgagctc tcctggcagg ccacgatcca
     5521 caatggccag gcggacgaaa ccgtgaccgg caccggaccc ggcaccaagc tcaccgtgac
     5581 ccggccccag gtcgagctca aggtgatccc cgacgtccag tccggaccgg ccccgctcac
     5641 cgtcaccacc gtctacctcc tgaccaacac gagcccggtc cccacggccg gaacgccggc
     5701 gccgggactt gtgcccaccg gatcgaacgg accacgagat gtcctggccg acgccggttg
     5761 cggacccctc aactaccttt ccggcgacct gccggccgac accgaccccg tgctcgaccc
     5821 gggggaaacc tggatcttca cctgtctcag gacctatccg ctgccgggca cctacgcgag
     5881 ccagcccgtg atcaccggcc taagcacggc cgatggaagg gcctggccgg agcaacccct
     5941 gggggcggag ccaccgtcag tgaccgcgct cgggccggac ctggtggtcg ccaagagcca
     6001 tcagggcgat ctgctggccg ggagttccgg ttcttacgaa ctggaggtca ccaacaccgg
     6061 aaaccagccg accgacgggg ccgtgaccgt cgcggatcag ctgcccgccg ggctgaccgg
     6121 caccgccatt gccggagacg gatggacctg caccctcgct tcgctgagtt gcagccggtc
     6181 ggatccgctc ggttccggca attcgtatcc ggaggtgacc gtgaccgtgg cggtcagcga
     6241 cgacccgcct gacaatctgg tcaacaccgc gaccgtgagc ggaggcggcg aacccgccgg
     6301 agcggtcgcc aacaatctgg atgaagatcc gaccacggtg cgaatcccct cgcagccgac
     6361 ccctccgtcg aacaggatct tcaaggttcg caaggtgacc agtcgcggcg acggttcggc
     6421 gctgatcaag gtcgccgtcc ccggagcagg agtggtggct gccgatgacg ccggaaagtc
     6481 cgacctggtt caacgggcat ggcaaaagag ctccaaagcc cgcactgttt cgctggtcgt
     6541 gaaagccgga agcaagctcc gcaaggctct ggtcgcgagc ggcaagccga ggcaggtccg
     6601 ggtgagaatc agcttcacgg cgaagggcag cccgaaggcc accccgacga cttcgaccgt
     6661 gcgttcgatc tcgttcctga tctcgccccc gcagacgctc gcgaattagc cgaacatcga
     6721 gttgagcgcg gccgtcgtgc cgctgatgat ttcgagatcg ctctgacggg aaagccggtg
     6781 ggcgtccctg ccgccggaga gggtgagcag gaacggctct ccggtgactg gcaccgcgtt
     6841 cacccaggca ccccagcgtc ccggtctccc cgggtcgatc cagctgaaac cttcggcgtt
     6901 ttcatcccag aacggttcct cgaaggccag ccagaccttg tcgagcaaac ccgccccgag
     6961 tccgtcgatc gcggtacggg tccgggccgg cagtggcggg tcgaactcga tcctttccgc
     7021 cttcagtacc ccgatcggaa ccgtgaccac ggccgcgtcg gccgcaactt catcgttgtc
     7081 ctctgccccg gtcagccgga cctcccccgg ccgctggctg atcttccgca cctcgaaccc
     7141 gaatcggatc ccggcgtcac cggacaggct tccgatcacc tgcgagtagg gcccgtccag
     7201 caaggcgtca cctccgggca gccagttgcc ttcccaggtc gcgagcgccg ccagctgatc
     7261 cgggtcggcc ccgtattcct gggcgacctc gtcctggatc gcccactcga gcccgggtcc
     7321 gcgccgccgc ggaaggattg cctcgagcgg aacgtccgga tggcggaccg agcggggacc
     7381 gaaggaattg actgcctgcc agtagcgatg ctcgcccccc gctccgagcg cctgctgccc
     7441 cggcaccggg aaatcgagct ggtacctgaa cgggtgaagc ccgacaccgg cctccccggc
     7501 gagttcggtc agcgggtttc cgtccacgcc gtggatccac gaggcaccaa gttcaaccgg
     7561 cgaaccgtcg aggtcggcct ccctgaccct tcctccgacc cggtccgaag cttcaagcac
     7621 caccggcttc aggccgaggc gttcgagttc accggcgcag gagaggccgg ccgccccggc
     7681 cccgaccacc gccacccgcg ccccggccct gagctgcccg gccgcgtact ttcctgcgtc
     7741 ccttcccgcc tggattgccc cgtgaaccgt ggccggaatc ctcgccgtgg cttccccggc
     7801 aaaggcgatc ctgccgaccg gctcggccag catccttctg atctccgaac cgtagggagt
     7861 cggggcgagg caggagtagg agcagaaggc cagcgggtca tgcgaccagt cggtccggat
     7921 gtagtcgacc ggcctgggga tcccggtttc cggaagaccg tcaatcacct cctgcttcga
     7981 ggctccggat ccgttgccac cgccttcccg ggcacaaccg gccagtccgg ccgctcctcc
     8041 gagcccggcc acagcggcgc ctgcgagggc cgccttgccc aggtcccgtc tggtcaactc
     8101 gaatcccacg ctccgattgt cgccgatgtc atgccgatcc ggtctgaaaa cgggaaaacc
     8161 cgccggcggg cgggctttcg gaggtaccgc tacggggatt cgaaccccgg tttccggact
     8221 gagaatccgg cgtcctagac ccctggacga tagcggcgcg cagcgagcat tctaacaatt
     8281 cggatgctca acggacggtc ggcggggtca cgccggggcc ttgcggagat caagcccgcc
     8341 cggccacagc cggagtgcgc cgcagcgggt caagccggta gagttccgcc tccgcgcggc
     8401 tgtggcggaa ctggtagacg cgcaggcttc aggtgcctgt gttcgcaaga acgtggaggt
     8461 tcgactcctc tcagccgcat tccccggaag gcccgctgct ggccggcttc cggcttagag
     8521 cggactggtg ccacaacggg tgaaccaggg aagctcgtca agggcggcgc ccacctcaac
     8581 cgtcctggcg ccctcgaccg gcgggccggt acctgaccgg cgccagcagc cggatggcag
     8641 cagcacttcc cgggtcaccg cgccctcctc gaggacgggc gcgaccagaa ggtcgtgacc
     8701 gaccatccat tcgtcatcgt tgtgcggatt ctttggtccg accccgccgg ccagatagag
     8761 gggcctgatc atcggcatgc cggttttcag gaaccgcttc cagagccttc tgatcaacgg
     8821 ccggctccgg ttatgcagga ccaccatctt cttccaggtt gactgagcgt cggtcgagaa
     8881 gtcccacggc atccgcgccc cgctcagacc ggaattgtga acccggaaga acggggtgaa
     8941 gacagcggcc tgggcccacc tcgtgaacag ttcttctccg gttggcggga tgaagggttg
     9001 ctcggtccgg aactgggagt agccgccgat gtcggtcgtg aaaccgaccg ccccggttag
     9061 cgcccggttc agcatgtccg gaactatcga aggcagcccc gtgtcgctgc cccagtcaac
     9121 cgtctcgtca cccgggaact gggcgctttc ccacttcacc gaactgagcg agccggtgaa
     9181 tccagcccgc tggaagaaga agatgtgccg tttcggatgc cggcgctccc aggcgtcaac
     9241 ggccgctcgt gtgacttttg cctgcagggc cgggtagcgg ttgtgcatca gcgcggcact
     9301 ggtcccgtcg tgaaatctca tgccggcttc gacctgttcg ccgaagtcat tcatgaaacc
     9361 gtcggcgccg aggtccaaca gattccagat ccggttccgc caccacctca ccgcggccgg
     9421 tttggtcagg tcaatcaccg ccgcctgacc gcccgggaag ggcgagggca aaaggaacgg
     9481 cttcccgtcc ttgccggtcg caaccagctt gcgggaaatc gcctggtcga aggtgccggg
     9541 gccttccgtg cccgcggtgt cattcgaaac gaacgagcgc agataaagca ccgggtggat
     9601 gccttcttcc ttcagtgtcc ggatcgtcgc tttgacgaag ctcccgggca gcgcctgcca
     9661 gccttcgaag gcgtaggccg agatcgggaa atccgcgctg gccagatgtt caaggtcgga
     9721 ctcgacccgc gactggtact tgccaccgga gtccgcggcg atcccgatcg cccgggaaag
     9781 aaccgttccg gtcgcccagg cgggcggcag gcggtgccta ccggtcagcc gggagattcc
     9841 ggccatcgcc cgggcctgac cgcccgaggt cagggtcatt tccatcgacc tgccgggccg
     9901 gctgacccgc caggcggttg gccggtcgct ggccatccgc cagcggctga tccggtcgcc
     9961 tttcagcagc aggccatagc cacgcgatga gatgaatccg ggaaccggag agtagtaggc
    10021 ggtggtcggg tccggatagc ggtagtcgag cacccagctc ttgatgtcgg aaccacgcag
    10081 gtcggttcct tcccggcggc cgccgaaacc gtggaaggcc tcgctttgcg gcgagcggaa
    10141 cccgaggtag acggagcctg ccttgcccgc catcatcccc gcccggattc ccagggttcc
    10201 gtcctttctc agccagatcg tcagccgcat ccgccggccg ccgggcgagt cggtccgtgg
    10261 ccgcgcctcg taaacatccg ggccgaacca cattccattt gccctggtcg ccacgtacga
    10321 ggtctgcgtg gacggctctt cgtcgggcgc cgccgacagg tccggcggtt cgagctcttt
    10381 cgccggttgc ggggtgtatc ccagggttgc gtagcgcacc ccgtttcgct cgaaactgtg
    10441 gcccagcccg gccagcaggt accggccgcc cctgcttatg ctgaagccga atggcgagtc
    10501 ggagaaccgg atctggtacg ggcccaggcg ccccgtcgtg aaaccgccct cgccggcatt
    10561 tgccgtcgcc ggaaccagaa tcgaaatcgc tatcgcgatc accgttgcca ggccctgcca
    10621 gcctctcccc tgccacatgc cccgaatcct acccgccggg tagtctggcg ggccgatgaa
    10681 gatcttctcc tggaacgtca atggcattcg ggccgtcatc cgcaagggtg acttcggctc
    10741 cttcgtcgag aagtacgacc ccgacatcct ctgcatgcag gagaccaagg cggagcaggg
    10801 ccaggccgag atcgacctcc cgcaatacga ggagtactgg aactcggccg ggaagaaggg
    10861 ctactccgga accgcgatct tcacccggca gaagccgatc gcggtcctga acgatttcgc
    10921 cgccgagatc tcggacgagt acgacctgca caacgattca tacggcgacc cgcgggccga
    10981 gggccgggta atcaccgccg aatacgacca cttcttcctg gttacgacct atgtgccgaa
    11041 cgccaagcgg gacctctccc ggctgaagct gcgcgagaag cagtgggatc cggccctgct
    11101 ccatcacatg aagatgctgg acgcgaaaaa gccggtcctg gtctgcggcg acatgaacgt
    11161 cgctcacacc gaggatgacc tggccaaccc gaaggccaat gccggcgagc acggcttcac
    11221 ggacgaggag cggcaggggt tccagaactt tctcgacgcc ggcttcctcg acaccttccg
    11281 cctcttcgcc gaaggcaacg gccattactc ctggtggtcg tacatgtcga aggcccggga
    11341 gcggaacgcc ggctggcgga tcgattactt ccttgcctcc gaacgcctca ggcaggcaat
    11401 caccggggcg gagatcctgg gtgatgtcat gggttccgac cactgcccgg tcagcgtcga
    11461 gctcgatgaa aagaaactgg cgaagctgta gggcagcccg accgtgaaga agggcggccg
    11521 cctaggccgc cgcccttccc gatacgcgtg cgtggcgcta tttcacccgg accctcgcaa
    11581 aggcggaggc ggacacgccg tttcccttga ccgtgaacct gatggtccgg gagtgacctt
    11641 tccggccgcg caccctggcc cggatgccga tcgtcctggc gacgccggga gcgatccagc
    11701 cgaccttctt cttgaccgag ggcgcaaaca ggtaaacgcc ctttgccgtg gccgttccgt
    11761 agttccttac atagacgcgg aagaaccccc tgccgccgtt ccggaccgcg cgcggcttct
    11821 tcacaacttt cgccttgagc ttggcgtgac cggccagacc gacgcagctc tgcatggtct
    11881 cgggggaagc gacccgggtg gtctgaccga cgtcggtgcc agccgggtag gtgcgctcgc
    11941 cgagatcgaa gacggcgttg gtcttcagcg ggctgctggc acacctggcc tggacatagt
    12001 tcgggtcgag accgcgaacg tggacgccga tgtcttcgcg atcaagtctg ccaccgggga
    12061 agttcagatc aaagtacttg acggccgagt cattgctcag caccggaacg gccatgctga
    12121 ggacgctccc cttcagggtg ccgttcataa gaatgccgac gttggtggtc ttcgagtagc
    12181 cgtagatctt gatcctgggc tgaccactgc ccgtgtaacc ggcgttgaaa gcaatcagga
    12241 tggggtcggt gatgagcgct gtcggcaggt tgaccttggc gaggtagatc gccgc
//
LOCUS       DAOQKN010000064        12027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_2476_length_12027_cov_5.800952, whole genome shotgun sequence.
ACCESSION   DAOQKN010000064 DAOQKN010000000
VERSION     DAOQKN010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 12027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12027
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2476_length_12027_cov_5.800952"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PLJ64_05890"
     CDS             <1..307
                     /locus_tag="PLJ64_05890"
                     /inference="COORDINATES: protein motif:HMM:NF015426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93048.1"
                     /translation="IDRFGPLPEDVENLITLQQARIDLGSAGVEQVQFRGGKLTVSGI
                     ELDSEAAAAIRESVPGALYEWRSHELSVRIDNDPAKRLATVSELGRALADVGMLRQS"
     gene            351..1625
                     /locus_tag="PLJ64_05895"
     CDS             351..1625
                     /locus_tag="PLJ64_05895"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNC93049.1"
                     /translation="MSGARKFGLILFGAILVVLFAGIAIAQGVSKPGVESGDIITVQD
                     VPDGKGSITEEDYNRSFEQTWKRGGLQAAPKEGDAQYDQVKEAAINDLLDQAWLAGEA
                     NELGVTATDREIDNELATIKKDQFPTPKAFNDFLKSSGFTMAEVRDRVELQVLSRKIQ
                     DQITKSVTDVPDSEIENFYESSKESFTTPETRDIRLIVTDKKADAEQAISELGDDPTD
                     EEFAKVAKKLSVHSSKTEGGKTVATEGAFPDPAGADIMSAEAGTIEGPIESGKQFYVF
                     RVTKITPEETKPLDDVRDQIKQQLLPTLQQQAMSDFVSDYNSKWKSRTFCASDYTVER
                     CDNFESDGRLESADPKCYEAGAGKNPDLACPAPIELPKPMAPGGNSSPGALLGQTQNL
                     PQRPVQPSDAESDAAGAGGAGALSQLGAAAGQ"
     gene            1658..2308
                     /locus_tag="PLJ64_05900"
     CDS             1658..2308
                     /locus_tag="PLJ64_05900"
                     /inference="COORDINATES: protein motif:HMM:NF015758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HNC93050.1"
                     /translation="MESGELAGALERLDRVTRELREKCPWDREQDARSIVPHTVEEAF
                     ELAEAAREGDDPAMRDELGDVLFQVVFLSLLMEERGEGDLARIANQLTDKLIRRHPHV
                     YPPEQSGRSEGIETADDVRRQWDEIKRDVEGRKSEKDWKKPALPALIYANKVQWKLDP
                     EERPANAGAANPDREQAEAEIGRLLWEAVAEARSRGVDPELALRAEAERHAETLQK"
     gene            2368..3660
                     /gene="eno"
                     /locus_tag="PLJ64_05905"
     CDS             2368..3660
                     /gene="eno"
                     /locus_tag="PLJ64_05905"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNC93051.1"
                     /translation="MSTIEAVHARQILDSRGNPTIEVEVTLENDFTGRAAVPSGASTG
                     EFEAVELRDGGDAYLGKSVNQAVANVNGEIREALLGFDVTDQAGIDKLMIDLDGTENK
                     GRLGANAILGVSLAAAHAAAAMEKRPLYSYLGDLYGESDPSLLPVPMMNVLNGGAHAD
                     NSVDFQEFMVMPAGTSSFSECLQVGTEVFHSLKKILSGHGLATAVGDEGGFAPDLSSN
                     EAALEMLIEGIEAAGYKPGEEVFIALDPATSEIYSDGVYRLEHEDRDLSPQQMADYWQ
                     SAAERFPIVSIEDGMDEEDWDGWKLLTEKLGDSVQLVGDDLFVTNPARLKRGIDLGVG
                     NSILVKVNQIGTLTETLEAIRMAADAGYSAVISHRSGETEDTTIADLAVATGAGQIKT
                     GAPSRSDRVAKYNRLLRIEEDLGERARFGGVSVLKRGN"
     gene            3694..4071
                     /locus_tag="PLJ64_05910"
     CDS             3694..4071
                     /locus_tag="PLJ64_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93052.1"
                     /translation="MSVRAHTYGDARRPPRRRPAPRRNAGKPGAGRIQWDRFGRIVLA
                     IVIVLLFAAMIKPLWNAVHTYRLAGQTKVRLHQAQAEQAMLQRQLRRASSESTLQREA
                     RRLGMIRPEESAWVVNGTKGDSR"
     gene            4097..>5571
                     /locus_tag="PLJ64_05915"
     CDS             4097..>5571
                     /locus_tag="PLJ64_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93053.1"
                     /translation="MSFADFIAGLVHLALVAGFTGFTAFRLRARLLPGWGGAPARLVE
                     AVAGVGLLVLAAELLGLFGWLEILPLEVLLGLIAVSAWLRLVPRRPGTGDSVPPSPAV
                     STGAQVLAFLAAFAVFAQWGAFTSYNLDHGITNFDSVWYHMPFAAEIARTGSVTGFFH
                     PETVFLNWFYPQNSELVHAVAIVLTGRDFVSIFINLGWLALGLLAGWCVGRPYGRPQL
                     TMLGVAVLMATHTLVVREPGTGKNDVMAAALILASVAIMLNRSTSRPDPPGRVSPDWA
                     MAAGGLAIGLAAGTKVTALAPAFLVTCAVIYATIPGRRLRASGVWLGAAFIGGGWWYL
                     RNLFAAGNPMPQITRIGPLDLPAPDRLQVGRSDFSVFHYLTDTAVWKDYFVPGLDQGF
                     GHVWPLLFALVVGGLFLVMFTAHGRLTRTHGAVALLAILAYLVTPLSAAGPEGDPSAF
                     AINLRFLIPALGLAVVLIPLSSWFDRGWRRLALGLILIGLF"
     assembly_gap    5572..5671
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5672..5968
                     /locus_tag="PLJ64_05920"
     CDS             <5672..5968
                     /locus_tag="PLJ64_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93054.1"
                     /translation="GFDAIGTCREFARAVNGARPDYLVTSPYLNFNEYSQPISSPETG
                     WAENDPALEIVEPRTKPPEAEKPVIVWRVTGPMDPALCSRLGPESNFIPGLKGE"
     gene            6004..6330
                     /locus_tag="PLJ64_05925"
     CDS             6004..6330
                     /locus_tag="PLJ64_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93055.1"
                     /translation="MAYPLENALFNWEAGIDRLRELERSGELRGDDVTVPVREELRRR
                     LGATFTATDLADLYGEGTDWALLLPGVDPGLSDVQELIDAAFWLHLQAAGDFAGGRVV
                     VDLELD"
     gene            complement(6412..7029)
                     /locus_tag="PLJ64_05930"
     CDS             complement(6412..7029)
                     /locus_tag="PLJ64_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93056.1"
                     /translation="MARRSLRPQLNQIRAWVRQGRTDAWIAHQLEVSASELREFRRQH
                     DLSPDDDADGTTEVDLRDEIEAEIEAAAAEEEAARKKAEEEASAAEAAKAAEREARGE
                     TDGEDDDDAEGESDSGEETETDGSEAEGRPRRRRRGRRGRRGRGGSNKLEGTFDHGDE
                     GYGLWLDPSIQDVPAYAENWAGKRDVVITVEADQIIIRRAGEEQE"
     gene            7163..7345
                     /gene="csrA"
                     /locus_tag="PLJ64_05935"
     CDS             7163..7345
                     /gene="csrA"
                     /locus_tag="PLJ64_05935"
                     /inference="COORDINATES: protein motif:HMM:NF002469.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HNC93057.1"
                     /translation="MLVLTRKKEQSIIIGENVEIMVLGVSGDKVRLGITAPREVDVFR
                     KEVIDERGSKAETENA"
     gene            complement(7388..7621)
                     /locus_tag="PLJ64_05940"
     CDS             complement(7388..7621)
                     /locus_tag="PLJ64_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93058.1"
                     /translation="MRQSANQFEAAFEQQKILEQRRREQLRNRAVNRSRARRVSRSQQ
                     QGKVRFSVLAVCLTVTVVVVTIVMFEALLFFMS"
     gene            complement(7776..8363)
                     /locus_tag="PLJ64_05945"
     CDS             complement(7776..8363)
                     /locus_tag="PLJ64_05945"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HNC93059.1"
                     /translation="MKQGNIPTPPGQFGNLFPDHSRGYLILFVVILAAFGYWLLNRDD
                     GNKDPGLQGSGSSVLRVDVDRVVDGDTALVWMDGHQQYVRYIGIDTPESVKEGYPIEC
                     YGPEAKQFNEELLDRRSTVKLVFDDELRDRYGRLLAYVYSGRTLLQAELLEGGYATTI
                     EVPPNTSRAGEFSAMESEARDAGRGGWSACPGFGR"
     gene            complement(8372..9292)
                     /locus_tag="PLJ64_05950"
     CDS             complement(8372..9292)
                     /locus_tag="PLJ64_05950"
                     /inference="COORDINATES: protein motif:HMM:NF013916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93060.1"
                     /translation="MEANTQTEQKSLLATLPPGKRARLHRLLFEFGPGNGTMMLLPID
                     QGIEHGPRDFFPNPASKDPEYQFKLAAEAGYSALACQIGMAEKYYPDYAGEVPLILKV
                     NGKTDIPSSAHAFSACNATVEDGVRLGADAIGYTLYVGSPRQDEDLAQLRQVRQDCDR
                     FGMPLVVWSYPRGEAIDAKGGAGSFYAIDYAARMAMEMGADVVKLNYPKIDPEKDKDA
                     QPPYNEMDVTAEEAMRQCVESAGRSLVVLSGGSKTDDETVLGHCKSVMNAGGSGVIFG
                     RNVWQREWSEALEIIAQIKETLLANVKRTP"
     gene            9406..10203
                     /locus_tag="PLJ64_05955"
     CDS             9406..10203
                     /locus_tag="PLJ64_05955"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNC93061.1"
                     /translation="MSETELNVVCKSCGAEVSPYVTECPYCGARLRKRAPDLELGGDG
                     GLEPKQSRREKRREEKARQKAARRSGPRPARAASLGVRPWATISLILIPAVALIVRIA
                     LGNALEDFGGVIVPVQSEWWRILTAPFAYLSVGYLFAVGLALALFGPGIERRFGTVPT
                     FLLLVACGALGVLAASAVADQREVLTVIAGGNGIALGAIGAWFMVARSESRATGDGFE
                     AAGAIVSAAVILLLPVVVPTADFWSGLAGGIVGVLAGFTATRFGSPR"
     gene            10221..10577
                     /locus_tag="PLJ64_05960"
     CDS             10221..10577
                     /locus_tag="PLJ64_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93062.1"
                     /translation="MPEEQISDEQLAAAVERLSDPERFREAEQLVSRVAPGLPAVLVT
                     ALGSGGWFGESHLSETLKAATIPDEDQRLTAIRALLTEETQMGMMVGVAVGWALRQEL
                     ENGDTPGAPAIKNEES"
     gene            10580..11296
                     /locus_tag="PLJ64_05965"
     CDS             10580..11296
                     /locus_tag="PLJ64_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNC93063.1"
                     /translation="MEIRYHGHSCFELSEGDTTLIVDPFLAPNNPIADVTADDVSVTH
                     LALTHGHADHVADAVAVATKNNAKCVAMVEVANWLEMQGVENVSDPNLGGTVRYDDDG
                     LSIKLVQAWHTNTLPGSDERPFSAELGTPIGTPSGLIIEMGGLTIYDAGDTCLFGDME
                     LIGKRHGVDIALLPIGGHYTMDRDDAAYAAGLIGAQRVIPIHYDTFPPIKTDAQAFAQ
                     DIQSKGMEAIVLEPGQTASL"
     gene            complement(11444..>12027)
                     /locus_tag="PLJ64_05970"
     CDS             complement(11444..>12027)
                     /locus_tag="PLJ64_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93064.1"
                     /translation="TVFRSGLFNCAAAVITAGAVEVKEKVSTSPVDTASPTTSKTIAD
                     SNPLQPVHVATPVNHSAPADVELDKASAEAPVTEPVDTTVPSQETDTTAVPVDHPAAE
                     TSAMPTEPEIPANPATSEGGDGLEGHTTYPPDEIVNEGPATVPSEGSTDTSGQIPGTA
                     PSTPVVTDPATPVVPTSPTPAPMPSAPQTAPVE"
BASE COUNT         1999 a         3938 c         3945 g         2045 t
ORIGIN      
        1 gatcgaccgt ttcggtcccc tgccggagga cgtcgagaac ctgatcacgc tccagcaggc
       61 ccggatcgac cttggctcag ccggggtgga gcaggtccag ttccgcggcg gcaagctcac
      121 ggtctccgga atcgagctcg attccgaggc ggcggccgcg atccgcgaat cagtgcccgg
      181 agcgctctac gaatggcgtt cccatgaact ctcggtccga atcgacaacg accccgccaa
      241 acggctcgca acggtgtccg aactgggccg cgcactggcc gatgtgggta tgctccgcca
      301 gtcgtagttt cagcgaccag ccacatcacc tttccaggag gagccactgc atgagcggcg
      361 cacgcaaatt cggtctgatt cttttcggag ccatcttggt ggttctcttt gcgggtatcg
      421 caattgcaca aggcgtcagc aagcccggcg ttgaaagtgg cgacatcatc accgtgcagg
      481 acgtgcccga cgggaagggc agcatcacgg aggaggatta caaccggtcc ttcgaacaga
      541 cctggaagcg cggcggcctg caggcggcac cgaaggaggg cgacgcccag tacgaccagg
      601 tcaaggaagc ggcgatcaac gacctgcttg accaggcctg gctggccggc gaggcaaacg
      661 aactcggcgt gaccgccacc gatcgtgaaa tcgacaacga actcgcgacg atcaagaagg
      721 accagttccc gacgcccaag gcattcaatg acttcctcaa gtccagcggc ttcacgatgg
      781 ccgaagttcg cgaccgggtc gaactccagg tcctgagccg caagatccag gaccagatca
      841 ccaagtccgt caccgatgtc ccggactcgg agatcgagaa cttctacgaa tcctcgaagg
      901 agagcttcac cacgccggag acccgggaca ttcgcctgat cgtcaccgac aagaaggccg
      961 atgccgagca ggcgatcagc gagctgggtg acgaccccac ggacgaggaa ttcgccaagg
     1021 tcgccaagaa gctttcggtc cactcgagca agaccgaggg cggaaagacc gtcgccactg
     1081 agggtgcttt ccccgacccg gccggcgccg acatcatgag cgccgaggca gggacgatcg
     1141 aaggtccgat cgagtccggc aagcagttct acgttttccg cgtcaccaag atcacgccgg
     1201 aagaaaccaa gccgctcgac gatgtccgcg atcagatcaa gcagcagctg ctgccgaccc
     1261 tgcagcagca ggcgatgagc gacttcgtgt ccgactacaa ctcgaagtgg aagtcccgca
     1321 ccttctgtgc gtccgactac acggtcgagc gttgcgacaa cttcgaaagc gacggtcgcc
     1381 tcgagtccgc cgatcccaag tgctacgagg ccggtgccgg caagaacccc gatctggcct
     1441 gcccggcccc gatcgagctg ccgaagccga tggccccggg tggcaactca tcgcccggtg
     1501 ccctgctcgg ccagacccag aacctgccgc agcggccggt ccagccttcc gacgcggagt
     1561 ctgacgcggc cggggccggc ggggcgggcg ctctgagcca gctcggcgcc gcggccggcc
     1621 agtagcaagc cgcggaccgg ccggcaggcg ggtggcaatg gaatccggcg aactcgccgg
     1681 agccctcgag cggctcgacc gggtaacccg ggaactccgc gagaagtgcc cctgggatcg
     1741 cgagcaggat gcccgctcga tcgtccccca tacggtcgag gaggcctttg agctggccga
     1801 ggcggcccgg gagggcgatg acccggcgat gcgggatgaa ctcggggacg tgctcttcca
     1861 ggtcgtgttc ctttccctgc tgatggaaga acggggtgaa ggtgacctgg cccggatcgc
     1921 caatcagctc accgacaagc tgatccgccg gcatccgcat gtctacccgc cggagcagag
     1981 cggcaggagc gaaggcatcg agaccgccga cgacgtccgc cgccagtggg acgagatcaa
     2041 gcgggatgtc gaaggcagga agtccgagaa ggactggaag aagccggccc tgccggccct
     2101 gatctatgcg aacaaggtcc agtggaagct tgacccggag gagcgccccg cgaatgccgg
     2161 tgcggccaac ccggatcgtg aacaggccga agctgaaatc ggacggctgc tctgggaagc
     2221 ggtggccgag gccaggagcc gcggggtgga cccggaactg gctttgaggg cggaggccga
     2281 aaggcacgcc gagactctcc agaagtaggc gcccgaggcc tgggccgggg gcggaggccg
     2341 ggcaacaatc tctagaatcg gcggctaatg agcacgatcg aagccgtcca cgcacgccag
     2401 atcctcgact cccggggcaa ccccacgatc gaggtcgagg tcactctcga aaacgacttc
     2461 accggccgcg ccgccgttcc ctccggcgcc tcgacgggtg agtttgaagc ggtggagctc
     2521 cgcgatggcg gtgatgccta cctcggcaag tcggtcaacc aggcggtggc caacgtcaac
     2581 ggcgagatcc gcgaggccct gctcggcttc gacgttaccg atcaggccgg catcgacaag
     2641 ctgatgatcg acctcgacgg aaccgagaac aagggccggc tcggcgccaa cgcgatcctc
     2701 ggcgtctccc tggccgctgc ccatgccgcc gcggcgatgg agaagcgccc gctttacagc
     2761 tacctcggcg atctctacgg cgagtcggac ccgtcgctcc tgccggtgcc gatgatgaac
     2821 gtcctcaacg gcggcgccca cgccgacaat tcggtcgatt tccaggaatt catggtcatg
     2881 ccggccggca cctcgagctt ctccgaatgc ctgcaggtgg ggaccgaggt gttccactcg
     2941 ctgaagaaga tcctctccgg gcacgggctg gccacggcgg tcggtgacga aggcggcttc
     3001 gccccggacc tctcctcgaa cgaggcggcg ctcgagatgc tgatcgaagg catcgaggca
     3061 gccggttaca agcccggcga agaggtcttc atcgcgctcg acccggccac ctccgagatc
     3121 tactcggatg gcgtctaccg gctcgaacac gaagaccgcg atctcagccc gcagcagatg
     3181 gccgattact ggcagtcggc agcggagcgc ttcccgatcg tctcgatcga ggacggcatg
     3241 gacgaggagg actgggacgg ctggaagctg ctgaccgaaa agctcggcga ttcggtccag
     3301 ctggtcggcg atgacctgtt cgtgaccaac ccggcccgtt tgaagcgcgg aatcgacctg
     3361 ggcgtcggca actcgatcct ggtcaaggtc aaccagatcg gcaccctgac cgaaaccctg
     3421 gaggcgatcc gcatggccgc cgacgccggc tactccgcgg tgatctccca ccgttccggt
     3481 gagaccgagg acaccacgat cgccgatctc gccgtggcga cgggggccgg ccagatcaag
     3541 accggggcgc cctcccggtc ggaccgggtc gccaagtaca accgcctgct ccggattgag
     3601 gaagacctgg gcgaacgggc ccgtttcggc ggcgtatcgg ttctgaagcg agggaattga
     3661 aggaattgac cgtccggcct agaagggtcg gacgtgtcgg tgcgcgcgca tacctatggc
     3721 gatgcccggc ggccgccacg ccgccggcct gcccctcgcc ggaacgccgg caagccgggc
     3781 gcgggccgga tccagtggga ccgcttcggc cgcatcgtgc tggcgatcgt gatcgttctc
     3841 ctgttcgccg cgatgatcaa gccactgtgg aacgccgttc acacctaccg gctggccggg
     3901 caaaccaagg tgcggctgca ccaggcccag gccgagcagg cgatgctgca gcgtcagctc
     3961 cggcgggcca gcagcgagtc cacgctccag cgcgaggcgc ggcgactggg gatgatcagg
     4021 ccggaggagt cggcctgggt cgtgaacggc accaagggcg actcccggta gggagtggcg
     4081 aggccggtgg cctcaattga gtttcgctga cttcatcgcc gggctcgttc acctggccct
     4141 ggtggcgggc ttcaccggtt tcaccgcgtt ccggctcagg gcccgtctgc tgcccgggtg
     4201 gggcggcgca cctgcccgtc tggtcgaggc ggtggccggg gtcggcctgc tggtcctggc
     4261 cgccgagctg ctcggcctct tcggctggct ggaaatcctg cccctggagg tcctgctcgg
     4321 actgatcgcg gtgtcggcat ggctccggct cgttccgcgg cggcccggga ccggtgactc
     4381 ggtcccgcca tccccggcgg tttccaccgg agcccaggtc ctcgccttcc tggcggcctt
     4441 cgccgtcttc gcccagtggg gcgccttcac ctcctacaac ctcgaccacg gcatcaccaa
     4501 cttcgattcg gtctggtacc acatgccgtt cgcggccgag atcgcccgca ccgggtcggt
     4561 gaccggcttc ttccatccgg agaccgtctt cctcaactgg ttctaccccc agaactcgga
     4621 actcgtccac gcggtcgcga tcgtcctgac tggtcgcgac ttcgtctcga tcttcatcaa
     4681 cctcggctgg ctggcgcttg ggctgctggc cggctggtgc gtcggccgcc cctacggccg
     4741 gccgcagctg accatgctcg gggtggccgt gctgatggcc acccacaccc tggtcgtgcg
     4801 ggagccgggc accggcaaga acgacgttat ggcggccgcc ctgattctcg cctccgtcgc
     4861 gatcatgctg aaccgctcga ccagccgtcc ggatccgccg gggcgggtct cgccggactg
     4921 ggcgatggcg gcggggggac tggcgatcgg cctcgccgcg ggaaccaagg tgacggccct
     4981 ggccccggcc ttcctggtca cctgcgcggt gatctatgcg acgatcccgg gccggcgtct
     5041 gcgggccagc ggagtctggc tcggcgccgc cttcattggc ggcggctggt ggtacctgag
     5101 aaacctgttc gcggccggca acccgatgcc gcagatcacc aggatcggcc cgctcgacct
     5161 gccggctccc gaccgcctcc aggtcggtcg ctccgacttc tcggtctttc attacctgac
     5221 cgacaccgcg gtctggaagg actacttcgt gcccggtctc gatcagggct ttggccacgt
     5281 atggccgctc ctctttgccc tggtcgtggg tggtctgttc ctggtcatgt tcaccgccca
     5341 cgggcgtctg acccgcaccc acggcgcggt cgccctgctg gccatcctgg cctacctggt
     5401 cacgccgctg agcgccgctg gaccggaagg cgatcccagc gccttcgcga tcaacctgcg
     5461 cttcctgatt ccggcactgg gcctggcggt ggtcctgatc ccgctttcct cgtggtttga
     5521 ccgcggctgg cgccgccttg ccctgggcct gatcctgatc gggctcttca tnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggcttcgac gcgatcggga cctgccggga
     5701 gttcgcccgg gcggtcaacg gggcccgtcc cgattacctg gtcaccagcc cgtacctcaa
     5761 cttcaacgag tacagccagc cgatctcctc tccggagacg ggctgggctg aaaacgatcc
     5821 cgccctcgaa atcgtcgaac cgcgaacgaa gcctcccgag gcggaaaagc cggtgatcgt
     5881 ctggcgggtg accggcccga tggacccggc gctctgttcc cggctcgggc cggagagcaa
     5941 cttcatcccc gggctcaagg gcgagtgaac cggccgaagc ggccggcctg ccatcatccc
     6001 cgcgtggcct atcccctcga gaacgccctc ttcaactggg aagccggaat cgaccggtta
     6061 cgcgagctcg agcgttccgg agagctgcgc ggtgatgacg tcaccgttcc ggtccgggag
     6121 gaactcaggc ggcggctcgg cgccaccttc accgccaccg acctggccga tctgtacggc
     6181 gagggcaccg actgggcgct gctcctgccc ggcgtcgatc ccgggctttc ggacgtccag
     6241 gagctgatcg acgccgcctt ctggcttcac cttcaggccg ccggagactt cgccggcggc
     6301 cgggtagtag tagacctaga actcgattag ccgccgagcg gagccctccc aaccatcagt
     6361 gaatttgatg ggagggcggg tttcccttgg tcggcaaagc cgaccttttg cttattcctg
     6421 ctcttcgccg gcgcggcgaa tgatgatctg gtcggcttcg accgttatga ccacgtcgcg
     6481 cttgccggcc cagttttccg cgtaggccgg gacatcctgg atcgacgggt cgagccagag
     6541 cccgtatccc tcgtcgccat ggtcaaaggt gccttcaagc ttgttgctgc ctccacgccc
     6601 gcgacggccg cgacggccgc gacggcggcg gcggggtcgg ccttcggctt cggagccgtc
     6661 ggtttccgtt tcctcgccgg aatcagactc tccttcggca tcatcgtcgt cttcgccgtc
     6721 ggtctcacca cgggcctcgc gctcggctgc tttggcagct tcagcggccg aggcttcctc
     6781 ttcggccttc ttgcgggccg cttcttcctc ggcggccgcg gcttcgatct cggcttcgat
     6841 ctcgtcgcgg agatctacct cggtcgtacc gtcggcgtca tcgtcgggcg aaaggtcgtg
     6901 ctggcggcgg aactcacgga gttcggaagc actcacttca agctggtggg cgatccaggc
     6961 gtcggtcctt ccctgacgga cccaggccct aatctggttg agttgggggc gtagtgatcg
     7021 gcgagccata taggttgccg agtctattga aacggcttgg aagtgcgttc tccgggtagg
     7081 ggttgaagaa acgaagtggc ccgtctgtct caatacctat tattaggtat cgaaccgacc
     7141 actcggagtc gaatggagtc acatgctggt actgaccaga aaaaaggaac agagcatcat
     7201 catcggtgaa aacgtggaga tcatggttct cggcgtttcc ggtgacaagg tgcgtctggg
     7261 catcaccgcg ccgcgggaag tcgacgtttt ccgcaaggaa gtcatcgacg agcgcggctc
     7321 caaggcggaa accgaaaacg cctgaacaga caggaacccg ggggctttgg ggagccctcg
     7381 gcttttccta ggacatgaag aagagcagcg cctcgaacat gacgatggtc acgacgacca
     7441 cggtcacggt gaggcagacg gcgagcacgc tgaagcgcac cttgccctgc tgctgggaac
     7501 gcgacaccct gcgggcacgc gaacgattca cagcccggtt gcggagttgc tcccgccggc
     7561 gctgctcgag gatcttctgc tgttcgaagg ctgcttcgaa ctgatttgcg ctttgacgca
     7621 ttctcatagt ggttcggccg ggtgaaagcg aactttcaga aacaggccgt ccgtccaccg
     7681 tagcaaatga ttggccgatc tcaagtcttc gttcacattt ctgtgactga ggtcacagat
     7741 tcctcacaga ccaggcggcc gaaacggcgg ccgttctacc gcccgaagcc cgggcaggcg
     7801 ctccagccac cgcggccggc gtcgcgggct tcgctttcca tcgccgaaaa ttctccggcc
     7861 cgggaggtgt tgggcggaac ttcgatcgtg gtcgcgtagc cgccctccag gagctcggcc
     7921 tgcagcagcg tgcggcccga gtagacatag gccagcagcc ggccataccg atccctcagc
     7981 tcatcgtcga agaccagctt cacggtcgaa cgccggtcaa gcagctcctc gttgaactgc
     8041 ttggcctctg gcccgtagca ctcgatcggg taaccctcct tgactgattc cggggtgtcg
     8101 atcccgatgt agcgaacgta ttgctggtgg ccgtccatcc agaccagggc agtgtcgcca
     8161 tccaccaccc ggtcgacatc gaccctgaga acagaagacc cggagccttg aagccccggg
     8221 tccttgttgc cgtcgtcccg gttcagcagc cagtaaccga aggccgcgag gatgacgacg
     8281 aagagaatca gatacccccg gctgtggtcc gggaagaggt tcccgaactg gccagggggt
     8341 gtcggaatgt tcccctgttt caaacgaccc cttacggagt gcgcttgacg ttggcgagca
     8401 gcgtctcctt gatctgggcg atgatctcca gggcctcgct ccattcgcgc tgccagacgt
     8461 tgcggccgaa gatcacgccg gaaccgccgg cattcatcac cgacttgcag tgaccgagca
     8521 cggtctcgtc gtcggtcttg gatccgccgg agagcacgac cagcgaacgg ccggccgact
     8581 cgacgcactg gcgcatcgcc tcttcggcgg tcacgtccat ctcgttgtac ggaggctgcg
     8641 cgtccttgtc cttctccggg tcgatcttcg ggtagttgag cttgaccacg tcggcgccca
     8701 tctccatcgc catgcgggcc gcgtaatcga tcgcgtagaa ggaaccggcg ccgcccttgg
     8761 cgtcgatcgc ctcaccgcgc gggtaggacc agacgaccag cggcatgccg aagcggtcac
     8821 agtcctggcg gacctggcgc aactgggcga ggtcctcgtc ctgccgcggc gagccgacgt
     8881 agagcgtgta gccgatcgcg tcggcgccga gccggacgcc gtcctcgacg gtcgcgttgc
     8941 aggcggagaa ggcgtgggcg gaggagggaa tgtcggtctt gccgttgacc ttgaggatca
     9001 gcggtacctc gccggcgtag tccgggtagt acttctcggc catcccgatc tggcaggcga
     9061 gggcggagta accggcctcg gcggccagct tgaactggta ctcgggatcc ttcgaggcgg
     9121 ggttcgggaa gaagtccctg ggcccgtgct cgatgccctg gtcgatcggc agcagcatca
     9181 tggttccgtt acccgggccg aactcgaaaa ggagccggtg aagccgggcg cgcttgcccg
     9241 gaggcagcgt cgcgagcagt gacttctgtt cggtctgggt attggcttcc attggcttct
     9301 cggtcctcct gggggattgg aaaaagtctc gacaggcagt atgtcaatgc cggagccgat
     9361 caggcaggca ggttttggcc cgcaggttct atcctcggtg atgcgatgag tgagaccgaa
     9421 ctcaacgtgg tctgcaagtc atgtggggcg gaagtcagcc cctacgtcac cgaatgcccg
     9481 tattgcggcg cccggctgcg caaacgcgcc cctgatctgg agctgggagg agacggcgga
     9541 ctcgagccca aacagtcgcg ccgcgagaag cgccgggaag agaaggcccg gcagaaggca
     9601 gcccgacggt ccgggccccg gccggcccgg gcggcttccc tcggcgtccg gccctgggcc
     9661 accatttccc tgatcctgat ccccgcggtg gccttgatcg tgcggatcgc gctgggcaac
     9721 gcgctcgagg acttcggcgg ggtgatcgtg ccggttcaga gcgagtggtg gcggatcctg
     9781 accgccccgt tcgcctatct gagcgttggc tacctcttcg cggtcggtct cgccctggct
     9841 ctgttcggcc cgggcatcga acgccgcttc gggaccgtgc cgacctttct gctccttgtg
     9901 gcctgcggcg cgctcggagt gctcgccgcg tcggcggtgg ccgatcagag agaggtgctg
     9961 accgtgattg ccggcggcaa cggcatcgcg ctgggcgcga tcggcgcctg gttcatggtc
    10021 gcccgatccg aatcccgcgc caccggggac ggtttcgagg cggccggagc gatcgtctcc
    10081 gccgcggtga tcctgctgct gccggtcgtc gtgcctaccg ccgacttctg gagcggcctg
    10141 gccggcggaa tcgtcggtgt cctggccggt ttcaccgcga cccgtttcgg ctcaccccgc
    10201 tgaccctcta ggctgacccg gtgcccgagg agcagatctc tgacgagcaa ctcgccgccg
    10261 cggtagagcg actgagcgac ccggaacggt ttcgcgaggc cgaacagctg gtctcccggg
    10321 tcgccccggg attgcccgct gtactcgtca cggccctcgg gagcggcggc tggttcggtg
    10381 aatcacatct ctccgagacc ctgaaggcgg ccacgatccc ggatgaagac cagcggctga
    10441 ccgcgatccg ggcactgctc accgaggaaa cccagatggg aatgatggtc ggggtagcgg
    10501 tcggctgggc cctgaggcag gaactggaaa acggtgacac ccccggtgcg ccggcaatca
    10561 agaacgagga gagctgaaca tggaaatccg ctatcacgga cattcgtgct tcgaactgag
    10621 cgaaggtgac acgaccctga tcgtcgatcc cttcctggcc ccgaacaatc cgatcgccga
    10681 cgtgaccgcc gacgatgtct ccgtaaccca tttggccctg actcacggtc acgccgacca
    10741 cgtggccgat gcggtcgcgg tggcgacgaa gaacaacgcc aaatgcgtgg cgatggtcga
    10801 ggtcgccaac tggctggaga tgcagggcgt cgagaacgtc tccgacccca acctcggcgg
    10861 gaccgtccgc tacgacgatg acggcctctc gatcaagctg gtccaggcct ggcacaccaa
    10921 caccctcccc ggctcggacg agcgtccctt cagcgccgag ctcggcaccc cgatcggcac
    10981 gccctccggc ctgatcatcg agatgggtgg cctgaccatc tacgacgccg gcgacacctg
    11041 cctcttcggt gacatggagc tgatcggcaa acgccacggc gtggacatcg ctctgctgcc
    11101 gatcggcggt cactacacaa tggaccgcga cgatgccgcc tatgcggccg ggctgatcgg
    11161 cgcccagcgg gtgatcccga tccactacga cacattccct ccgatcaaga ccgatgccca
    11221 ggcctttgcc caggacatcc agtcgaaggg aatggaagca atcgtcctcg agcccggcca
    11281 gaccgcctct ctctaactgc cccaagacgg agccgggcca tccatttctg gatggccctg
    11341 gcccggcggg ttgagattgg aggcccggcg cagaccgggc cgactgttga tctgatggcc
    11401 ctggcccggc gggttgagat tggaggcccg gcgggccgac tccttattcc accggtgcgg
    11461 tctggggagc gctcggcatc ggggccgggg tcggcgaggt cggcaccacc ggggtggccg
    11521 gatcggtcac caccggggtc gaaggcgcgg tacccgggat ctgccccgag gtgtcggtcg
    11581 aaccttcgct cggcacggtc gccgggcctt cgttcacgat ctcgtcgggc gggtaggtgg
    11641 tgtgcccctc gagaccgtcg ccaccttcag aggttgcggg gttggccggg atctccggtt
    11701 cggtcggcat ggccgaggtc tcggcggccg gatggtcgac cgggacggcc gttgtatcgg
    11761 tttcctggct ggggaccgtg gtatcaaccg gctcggtcac cggcgcctcc gccgaagcct
    11821 tgtcgagctc gacgtcggcc ggcgcggaat ggttcaccgg agtggcgacg tgaaccggct
    11881 ggagcggatt gctgtcagcg atcgtcttcg aggtcgtcgg tgaggcagtg tcaaccgggc
    11941 tggtcgaaac cttttccttg acttccacgg cgccggccgt gatgacggcg gcggcgcagt
    12001 tgaagagtcc gctgcggaag acagtgc
//
LOCUS       DAOQKN010000065        12013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_2481_length_12013_cov_6.933266, whole genome shotgun sequence.
ACCESSION   DAOQKN010000065 DAOQKN010000000
VERSION     DAOQKN010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 12013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12013
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2481_length_12013_cov_6.933266"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..614)
                     /locus_tag="PLJ64_05975"
     CDS             complement(33..614)
                     /locus_tag="PLJ64_05975"
                     /inference="COORDINATES: protein motif:HMM:NF012564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93065.1"
                     /translation="MIATIAKAFSVPEIRRKLLFAAAMLALYRLGAYIPAPGIDVDAV
                     KELESQLTGSNILGFLNLFSGGSLSRLSLFALGIMPYITASIILQLLTVVVPSLERLQ
                     KEGEVGQQKITQYTRYLTVGLAAMQSFAYVFLFNSLSAGGNSVVSNMNLANVLLIVIS
                     LTAGTVLLMWIGELITQRGIGNGISLLIFSIER"
     gene            complement(614..1081)
                     /gene="rplO"
                     /locus_tag="PLJ64_05980"
     CDS             complement(614..1081)
                     /gene="rplO"
                     /locus_tag="PLJ64_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNC93066.1"
                     /translation="MGLHNLSPKPGSRRPKKRIGRGPGSGTGKTSGRGHGGQGQRSGN
                     KRKVNLEGGQTPIHMRMRKLRGPHMKKSMPFENFRTATQPVNLGDLEDRFDAGAEVTP
                     ETLKAAGLATRKDPVKILARGEISKSLTVKAHGFSAAAKEKIEAAGGSCELID"
     gene            complement(1114..1293)
                     /gene="rpmD"
                     /locus_tag="PLJ64_05985"
     CDS             complement(1114..1293)
                     /gene="rpmD"
                     /locus_tag="PLJ64_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HNC93067.1"
                     /translation="MAKLAIKQIRSDINTNPSQRGTLAALGLGKIGRKVERDDSPQLQ
                     GQLKVVSHLVEVEEA"
     gene            complement(1755..2240)
                     /gene="rpsE"
                     /locus_tag="PLJ64_05990"
                     /pseudo
     CDS             complement(1755..2240)
                     /gene="rpsE"
                     /locus_tag="PLJ64_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002063426.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
     gene            complement(2297..2650)
                     /gene="rplR"
                     /locus_tag="PLJ64_05995"
     CDS             complement(2297..2650)
                     /gene="rplR"
                     /locus_tag="PLJ64_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNC93068.1"
                     /translation="MSITTSRQRRQRRRYRVRAKVSGTAQRPRLSVYRSNRGVFAQLI
                     DDVDGRTLAAANWTEPELKKLSGTDQAKKAGELLAERAKKAGVETCVFDRSGYRYHGR
                     VKALAEGAREGGLEF"
     gene            complement(2647..3180)
                     /gene="rplF"
                     /locus_tag="PLJ64_06000"
     CDS             complement(2647..3180)
                     /gene="rplF"
                     /locus_tag="PLJ64_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNC93069.1"
                     /translation="MSRIGKKPIDLPDGVEIDIKPGLITVKGPKGELTQEVSTDMTVK
                     TAEGVISVERPTNRPNHRALHGLTRSLIANMIVGVTDGYTKVLEIQGVGYRAAAKGKN
                     LELALGYSHPVSIEAPEGIEFELPQPTEIIVKGIDKQKVGQVAANIRESRPPEPYKGK
                     GIRYRGEHVARKVGKRV"
     gene            complement(3191..3586)
                     /gene="rpsH"
                     /locus_tag="PLJ64_06005"
     CDS             complement(3191..3586)
                     /gene="rpsH"
                     /locus_tag="PLJ64_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNC93070.1"
                     /translation="MLTDPISDYLTRIRNGLSSGLATVEVPSSKLKLEVSRILTEQGY
                     ISGYEKSAADVGEKITVELKYTKDHQPVITGLERVSRPGRRRYVGHGEVPRVHGGTGT
                     AIITTSHGVMTGHEAKKEGVGGEVLAYVW"
     gene            complement(3591..3776)
                     /locus_tag="PLJ64_06010"
     CDS             complement(3591..3776)
                     /locus_tag="PLJ64_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HNC93071.1"
                     /translation="MAKTSQKVRQQRKAKFSTREYNRCRRCGRPRAYYRKFGICRICL
                     REAAHEGYIPGMTKSSW"
     gene            complement(3986..4555)
                     /gene="rplE"
                     /locus_tag="PLJ64_06015"
                     /pseudo
     CDS             complement(3986..4555)
                     /gene="rplE"
                     /locus_tag="PLJ64_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932832.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
     gene            complement(4564..4890)
                     /gene="rplX"
                     /locus_tag="PLJ64_06020"
     CDS             complement(4564..4890)
                     /gene="rplX"
                     /locus_tag="PLJ64_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNC93072.1"
                     /translation="MRIRTDDTVQVISGKEKGKQGKVLRTDPKAGTVYVEDLNMIKRH
                     QRAQPSPDGRGQGKEGGIIEMEGPIHVSNVALVDPTDNKPTRIGVRITDDGVRQRYSK
                     RTGEAI"
     gene            complement(4890..5258)
                     /gene="rplN"
                     /locus_tag="PLJ64_06025"
     CDS             complement(4890..5258)
                     /gene="rplN"
                     /locus_tag="PLJ64_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNC93073.1"
                     /translation="MIQQESRLRVADNSGARELLCIRVKGGSHRKYGSIGDVITATVK
                     QASPQGNVKKGEVVQAVIVRTKKEIGRQDGTYIAFDENAAVLIDAQNNPRGTRIFGPV
                     ARELRDRDFMKIISLAPEVL"
     gene            complement(5262..5567)
                     /gene="rpsQ"
                     /locus_tag="PLJ64_06030"
     CDS             complement(5262..5567)
                     /gene="rpsQ"
                     /locus_tag="PLJ64_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002514860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNC93074.1"
                     /translation="MKKERTGDGVGTPRAEKTRTGKPKERQGKVVSDKTDKTITVQID
                     TARRHPVYEKIVRQSHKIHAHDERNEAHEGDVVRVIETRKLSRTKHWKLVEVVEKAR"
     gene            complement(5910..6125)
                     /gene="rpmC"
                     /locus_tag="PLJ64_06035"
     CDS             complement(5910..6125)
                     /gene="rpmC"
                     /locus_tag="PLJ64_06035"
                     /inference="COORDINATES: protein motif:HMM:NF013027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNC93075.1"
                     /translation="MKSAEAHSMSDIELVEKLKEVREESFNLRLRHATGELEDTAALG
                     RAKKDLARLLTIAHQRNIDLERESKHK"
     gene            complement(6122..6541)
                     /gene="rplP"
                     /locus_tag="PLJ64_06040"
     CDS             complement(6122..6541)
                     /gene="rplP"
                     /locus_tag="PLJ64_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNC93076.1"
                     /translation="MLMPKRVKHRKVMRGRRKGNSKGGTAVTFGEYGLKSLDRGWITN
                     RQIEAARVAMTRKMKRGGKVWINVFPDKPITKKPAETRMGKGKGNPEAWVAVVKPGRV
                     MFEVSGVPEELAREAMRVAGHKLPVKTKFVQREGAGE"
     gene            complement(6541..7263)
                     /gene="rpsC"
                     /locus_tag="PLJ64_06045"
     CDS             complement(6541..7263)
                     /gene="rpsC"
                     /locus_tag="PLJ64_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009388013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNC93077.1"
                     /translation="MGQKIHPEGFRVGYIHDWKSNWFADKDFSDLLMQDLAIRDHIEN
                     KLAHAGLSDITIERRGEVAVDIHTARPGIVIGKSGSEVDALRKDLHKITGSPVKVNIR
                     EVKRPELDAKLVAQSIAEQLQNRVAFRRAMKRALTSAMRSGAKGVKVQISGRLGGAEM
                     ARTEGYSDGRVPLHTLRADIDYGFVEAKTGTGRIGVKCWINKGEVMPEGFESSRTSDE
                     APMSDRPDRRGGRGDDRRGGRR"
     gene            complement(7268..7651)
                     /gene="rplV"
                     /locus_tag="PLJ64_06050"
     CDS             complement(7268..7651)
                     /gene="rplV"
                     /locus_tag="PLJ64_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003567555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNC93078.1"
                     /translation="MAAKTTRKEEAAEPVLVRATSKYVRVSPRKARLVADQVRGKHID
                     DARSLLQFSPRSVADDISKVIESAAANAEANHDLIGDEMIVAEIRVDEGPTLKRFRPR
                     AMGRATPIHKRTCHISVALTPEDEE"
     gene            complement(7651..7917)
                     /gene="rpsS"
                     /locus_tag="PLJ64_06055"
     CDS             complement(7651..7917)
                     /gene="rpsS"
                     /locus_tag="PLJ64_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNC93079.1"
                     /translation="MGRSTKKGPYVEERLMGRIEAMNEAGNKNMVKTWSRRSTIFPEM
                     VGHTIAVHDGRKHVPVFISESMVGHKLGEFAPTRTFKSHTGGKD"
     gene            complement(7921..8766)
                     /gene="rplB"
                     /locus_tag="PLJ64_06060"
     CDS             complement(7921..8766)
                     /gene="rplB"
                     /locus_tag="PLJ64_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNC93080.1"
                     /translation="MAIRKTKPTSPGRRFATYQLREQITATEPEKSLIEGIKKSGGRN
                     NNGRITSRHRGGGAKRRYRKIDFKRTKDGIPAKVATVEYDPNRSAYIALLHYADGEKA
                     YILAPSQIEVGNQVSSGPGSDIRPGNCLALGEMPVGTTVHNVELKPGQGGKLGRSAGT
                     AIQLVAKEGDMVTLRLPSSEMRMVRAECRATIGTISNAEHQNVKIGKAGRNRHKGKRP
                     QSRGIAMNPVDHPHGGGEAHKTPGGHPVTPWGKPTLGYRTRKKGKQSDRYIVRGRRRG
                     KKKGR"
     gene            complement(8790..9083)
                     /gene="rplW"
                     /locus_tag="PLJ64_06065"
     CDS             complement(8790..9083)
                     /gene="rplW"
                     /locus_tag="PLJ64_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNC93081.1"
                     /translation="MDPRSVIIRPVISEKAYALIAANKYTFRVDDRAHKFEIKDAVEK
                     AFGVEVVKVRTSKVRSKPKRRGLHQGRSRSWKKAIVELAPGDSIELFEGAAVE"
     gene            complement(9083..>9472)
                     /locus_tag="PLJ64_06070"
     CDS             complement(9083..>9472)
                     /locus_tag="PLJ64_06070"
                     /inference="COORDINATES: protein motif:HMM:NF012782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL4 family ribosomal protein"
                     /protein_id="HNC93082.1"
                     /translation="PKPRHYTVKVNRKARRKAMRSALSVHAERGSVAVVKAADFETPS
                     TRDAAAALEKWGAKRSTLVIILGEETGLLKSFRNIPRVSVMEVGATGVVDVIGAASIV
                     VSEAALEVLAAQSTEPDRGTGTEKDGE"
     assembly_gap    9473..9482
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9483..9736)
                     /gene="rplD"
                     /locus_tag="PLJ64_06075"
     CDS             complement(<9483..9736)
                     /gene="rplD"
                     /locus_tag="PLJ64_06075"
                     /inference="COORDINATES: protein motif:HMM:TIGR03953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNC93083.1"
                     /translation="MASAKAPVLGKTTKADLPAGLFDEAFHQSLVHETARADANARRQ
                     GTASTLTRGEVSMTTAKAWRQKGTGRARVGALSVPNRYGGG"
     gene            complement(9729..10217)
                     /gene="rplC"
                     /locus_tag="PLJ64_06080"
     CDS             complement(9729..10217)
                     /gene="rplC"
                     /locus_tag="PLJ64_06080"
                     /inference="COORDINATES: protein motif:HMM:TIGR03625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNC93084.1"
                     /translation="MGHLAKADANPMRVLVEFRDVELPAAGSAGEEGSEGEEAEGDGG
                     EVKLGQQVTVEAFEEGQKVKVAGITIGKGFQGTVKRHNFGRGPVSHGSHNVRAPGSIG
                     ASAWPARVFKGMRLPGQMGGKRRTQLGLEIAGIDADRNLLMIKGSVPGAKNSIVEIRT
                     DG"
     gene            complement(10181..10453)
                     /locus_tag="PLJ64_06085"
     CDS             complement(10181..10453)
                     /locus_tag="PLJ64_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93085.1"
                     /translation="MSAILGKKLGMTQLFREDGEVVPVTVIEAGPCPVTAIRNEERDG
                     YSAIQLAFDEVKESKLSKAEVGHLAKLSRSSARPKWATWPRPTPTR"
     gene            complement(10459..10770)
                     /gene="rpsJ"
                     /locus_tag="PLJ64_06090"
     CDS             complement(10459..10770)
                     /gene="rpsJ"
                     /locus_tag="PLJ64_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNC93086.1"
                     /translation="MAAQKIRIRLKAYDHDAIDRAAREIVDTAERTGATVSGPVPLPT
                     ERNIYCVIRSPFKDKDSREHFEIRTHKRLIDIKQPTPKTVDSLQRLDSLPAGVDIEIR
                     L"
     gene            complement(11076..>12013)
                     /gene="tuf"
                     /locus_tag="PLJ64_06095"
     CDS             complement(11076..>12013)
                     /gene="tuf"
                     /locus_tag="PLJ64_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HNC93087.1"
                     /translation="HADYVKNMITGAAQMDGAILVVSAADGVMPQTREHILLARQVNV
                     PHVVVFLNKVDQVDDEELIELVEEEVRDLLNEYEFDGDNTPIIKGSALKALEGDADFQ
                     ARIVELGEALDSYIPEPERDLDKPFLMPIEDIFSITGRGTVATGRIEQGIVNTGDEVE
                     IVGIHDKTETTTITGVEMFRKILDEGRAGDNVGCLLRGTKREEIERGQVLCKPGSITP
                     HTKFRAEVYCLKKEEGGRHTPFFSGYRPQFYFRTTDVTGVANLPEGTEMVMPGDNVEM
                     TIELIQPIAMDQGLRFAIREGGRTVGSGVVSEIIE"
BASE COUNT         1724 a         3828 c         3928 g         2523 t
ORIGIN      
        1 ttcctgatcg gaccggcggt tccggccgag agttacctct cgatcgagaa gatcaggagt
       61 gaaatgccgt tgccgatccc gcgctgggtg atcagctcgc cgatccacat cagcaggacg
      121 gttcccgcgg tcagcgagat cacgatcagc agaacgttcg cgaggttcat gttcgaaacg
      181 acgctgttgc cgccggccga gagcgagttg aagaggaaga cgtaggcgaa ggactgcatc
      241 gcagccaggc cgacggtcag gtaacgcgtg tactgcgtga tcttctgctg gccgacttcg
      301 ccttccttct ggagacgctc cagcgaaggc acgaccacgg tcaggagctg caggatgatc
      361 gaggccgtga tgtagggcat gatgcccagc gcgaaaagcg aaagcctcga gagcgagccg
      421 cccgaaaaca ggttcaggaa gccgaggatg ttcgatccgg tcagctggct ttcgagttcc
      481 ttgacggcat cgacgtcgat gccgggggcc ggaatgtagg caccgagccg gtacagggcc
      541 agcatggccg ctgcgaagag aagcttccgg cgaatctccg ggacggagaa tgctttcgca
      601 atcgttgcga tcatcagtcg atcagctcgc agcttccgcc ggcggcttca atcttctcct
      661 tggccgcggc cgaaaagccg tgggccttga cggtcaggct ctttgaaatc tcgccgcggg
      721 cgaggatctt gaccggatcc ttgcgggtcg ccagaccggc agccttgagc gtttccgggg
      781 tgacttccgc accggcgtcg aagcgatctt cgagatcgcc aaggttgacc ggctgggtcg
      841 cggtccggaa attttcgaac ggcatcgact tcttcatgtg cgggccgcgc agcttgcgca
      901 tccgcatgtg aatcggggtc tggccacctt cgaggttgac cttccgcttg ttgcccgagc
      961 gctggccctg gccgccgtgt ccgcggccgg aggtcttgcc ggtgcccgaa ccgggtccgc
     1021 ggccgatccg cttcttcgga cggcgcgagc cgggcttggg gctcagattg tgaaggccga
     1081 tttcttcttc gcggctttcc tgcttgtcag ccatcaggcc tcctccacct cgaccaggtg
     1141 ggaaaccacc ttgagctggc cctggagctg gggggaatca tcccgctcga ccttgcggcc
     1201 gatcttgccc aggcccagag cggcaagcgt gccacgctgg ctcgggttcg tgttgatatc
     1261 tgaacgaatc tgcttgatcg cgagcttggc catcaggcgt tgtcctcttc agccttctcg
     1321 gcgtcctcgg ccttttcttc gctcgcctgt acttcttcgg cggcttcaac cacggtctcg
     1381 gcctgcgctt catcggcagc gacctcggcc tctacctcag aggcagcggg accggtcggc
     1441 tcgggggccg gggcgtcggt gccggctcca tcagttgcct cttcggccgg caccttcgcg
     1501 tcagcagtgt cctcggcagc cttctcctcg accgcggcga tgtcagccgg ggtttcggtg
     1561 gtctcggcgg cctctgcagc cggagcttcg tcggcttcgg tcgcggcgtc ctcgacgagg
     1621 gcttcctcgg cggggagttc ggcggcagca gtttcctgat cgctggcagc ctcgggtgcg
     1681 gaaccgttgc cgttgccgtt ggcagcggct tcctcggtgg cttcctcggc cttggcgtcc
     1741 ttgtcgatgc ccaggacctc gctgatcgtg agaccacgca gttcggcgac ctcttccggc
     1801 cggcgcagac ggatgatgcc gtccatggtc gccttgacca ggttgatcgg gttctgggtg
     1861 ccgacggact tggtgaggat gtcgcgcacg ccgcccagtt cgagcacggc acggacgccg
     1921 ccgccggcga taacgccggt tcccgggctg gccgggcgca gcaccacgtg accggcgccg
     1981 tagcggccga tgatcttgtg ggtgatcgtg tccccgtact tggggatccg aaccaggttc
     2041 ttgcgggcgt tctcgacggc cttctggatc gcgagcggaa cctcgcgggc cttgccgtaa
     2101 ccgatgccga ctaccgactc ttcgtcacct actgcaacga gggcagtgaa cgagaagcga
     2161 cgaccaccct tgaccacctt ggccacacgg ttgatctcga tcacacgctc ctgcaggtcg
     2221 agccccttcg ggctgaccat ctcaatgcgg gctgtatcca ttcccttcat ttcagctctt
     2281 caccgtaccg gagttcctag aactccagtc caccctctct cgcaccttcg gccagggcct
     2341 tgaccctgcc gtggtagcgg taaccgcttc ggtcgaagac acaagtctcg acgcctgcct
     2401 tctttgcacg ttctgccagc agttcgccgg ccttcttcgc ctgatcggtg ccgctgagct
     2461 tcttgagctc gggctcggtc cagttcgcgg cagccagcgt cctgccatcg acatcgtcga
     2521 tgagctgggc gaaaacgcca cggttggacc ggtacacgga caggcggggc cgctgggcgg
     2581 ttccggacac cttggcccgg acccggtagc ggcggcgctg ccttctctgg cggctggttg
     2641 tgatgctcat actcgcttgc cgaccttcct ggcgacgtgt tcgccgcggt aacggatgcc
     2701 cttgcccttg taaggctccg gcggacggct ctcgcggatg tttgccgcga cctggccgac
     2761 cttctgcttg tcaatcccct tgacgatgat ctccgtcggc tggggaagtt caaattcgat
     2821 gccctcggga gcctcgatcg agaccggatg cgaataaccg agcgccagtt cgaggttctt
     2881 gcccttggcc gcggcacggt aaccgacgcc ctggatctcc aggaccttgg tgtagccatc
     2941 ggtgacgccg acgatcatgt tggcgatcag gctgcgggtc aggccgtgga gcgcacgatg
     3001 gttggggcgg ttcgtcggtc gctcgaccga gatcacgcct tccgccgtct tcacggtcat
     3061 gtcggtcgag acttcctggg tgagctcgcc cttcgggccc ttgaccgtga tcagtccggg
     3121 cttgatgtcg atctcgaccc catcggggag atcgatcggc ttttttccaa ttcgactcat
     3181 gaattcctcg ttaccagacg taggcgagaa cctcgccgcc gacgccttcc ttcttggctt
     3241 cgtgaccggt catcacaccg tgagaggtgg tgatgatcgc ggtgccggtg ccgccgtgaa
     3301 cccgggggac ctcgccgtgg ccgacgtaac gacggcggcc ggggcgggaa acccgctcga
     3361 gtccggtgat caccggctga tggtccttcg tgtacttgag ctcgaccgtg atcttctcgc
     3421 ccacgtcggc agcgctcttc tcgtagcccg agatgtagcc ctgctcggtg aggatgcggg
     3481 agacctccag cttcagcttc gagctgggca cctcgacggt ggccaggccg gagctgaggc
     3541 cgttgcgaat gcgggtcagg tagtcggaaa tcggatcggt aagcatttga ctaccagctc
     3601 gacttcgtca tgccggggat gtagccctca tgggcggcct cgcgcaggca gatccggcag
     3661 atgccgaact tgcggtagta cgcacggggg cggccgcacc gccggcaccg gttgtactcg
     3721 cgggttgaaa acttcgcctt gcgctgctgg cgcaccttct gtgacgtctt ggccattagg
     3781 cctggtcctc ctgctcttcg gcggctgcgt cttcggcggc atcgtccgat gcctcctcgg
     3841 ccgcgtcatt ttcagtgtct gctgcttctg ccggggcctc ttcttcggcc ggcgcttcgg
     3901 tggagccggc tccatcagtt gcctccgcag ccggctcatc cggggcggtg ctttcttcgg
     3961 caccctcggg gaccggacgg ccttccttgg cgaaaggcat gcccaggccg agcagcagct
     4021 cgaaagcctc gtagtccgtc ggtgcggacg tggtaatcgt gatgtcgaga ccgcgggtct
     4081 cgtcaaccga gtcgtaatcg atctcgggga agatcagctg ttccttgaca cccatggagt
     4141 agttgccgcg accgtcgaat gaggtcgcct tgagtccgcg gaagtcacgg atgcgcggga
     4201 tcgcgatcga gttcagtcga tcgaggaact cccacatccg ggcgccccgg agggtcaccg
     4261 aggcgccgac cggcattccc tcgcgaagct tgaaggacgc gattgacttc ttggcgatcc
     4321 gggtgttcgg catctgaccg gcgatcgtgg cgagctcttc gatcgccttt tcgcgggact
     4381 tggagtcaac cgaggagccg aggcccatgt tcagggtgat cttctgaagg cgcggggccc
     4441 gcatcggagt cgagtagccg aaggtctcca tcagcttcgg gagaacctcg ttgatgtagg
     4501 tgtcccggag ccgcggcggc ggcactgcct gttgtgttgt cgtagctgct tccatgatct
     4561 tctctaaatg gcctcgccgg tgcgcttcga gtagcgctgg cgaacgccgt cgtcggtgat
     4621 gcggacgccg attcgggtcg gcttgttgtc ggtcgggtcg accagggcca cgttcgacac
     4681 gtggatcgga ccctccatct cgatgatccc gccttccttg ccctggcccc ggccgtccgg
     4741 cgagggctgg gcgcgctggt ggcgcttgat catgttgagg tcttcaacgt acacggttcc
     4801 ggccttgggg tcggtcctca gaaccttgcc ctgcttgccc ttctccttgc cgctgattac
     4861 ctgcacggtg tcatcggtgc ggatacgcat cacagcacct ccggggcgag cgagatgatc
     4921 ttcatgaagt cgcggtcacg cagttcgcgg gcgaccggtc cgaagatgcg ggtaccgcgc
     4981 gggttgttct gcgcgtcgat cagcacggca gcgttctcgt cgaaggcgat gtaggtgccg
     5041 tcctggcggc cgatctcctt cttggtccgg acgatcacgg cctggacgac ctcgcccttc
     5101 ttgacgttgc cctgcgggct ggcctgcttg acggtcgcgg tgatcacgtc accgatcgat
     5161 ccgtacttgc ggtgcgagcc acccttgacc cggatgcaga ggagttcacg ggcgccggag
     5221 ttgtcggcga cgcggagtct ggattcctgc tggatcaccg tctacctcgc cttctccacg
     5281 acctcgacca gcttccagtg cttggtcctg gaaagcttgc gggtctcgat cacgcggacc
     5341 acgtcaccct catgggcttc gttccgttcg tcatgcgcgt ggatcttgtg cgactggcgg
     5401 acgatctttt cgtagaccgg gtgacgccgg gcggtgtcga tctggaccgt gatggtcttg
     5461 tcggtcttgt cggaaacgac cttgccctgg cgctccttcg gcttgccggt acgggtcttc
     5521 tcggcccgcg gggtaccgac accgtcaccg gtgcgttcct tcttgatctc ctcgcggcga
     5581 ttgcgacgga tctgcgcctt cttcttccgg gcagcgacgc gatcggactg gcgctgctcg
     5641 cccgaacgtt cggggccggc ctcgccggat tcacgcgact tgagcacgcg gcggcgttcc
     5701 ttccagggga gctcgtccag cggatcgggc tcagcctcgg cggcttcctc ggcgggagcc
     5761 tccggggcgg gagcttcctc ggccggagct tcttcggcag cgggagcctc ttcagccgga
     5821 gcttcggctt ccggggtctc ctcgaccgct tcggccggcg cctctgtgtt gttctcttct
     5881 tctacgtttt cttcttctga catgggttcc tacttgtgct tcgactcgcg ctcgaggtcg
     5941 atattgcgct ggtgggcgat ggtcaggagc cgggcgagat cctttttggc gcggccgaga
     6001 gcggccgtgt cttcaagttc gccggtcgca tgacgcaggc gcaggttgaa ggactcttcg
     6061 cggacttcct tgagcttctc gaccagttcg atgtcactca tgctgtgggc ttcggcggat
     6121 ttcactctcc cgctccttcc cgctggacga acttggtctt gaccgggagc ttgtgaccgg
     6181 cgacgcgcat cgcttcgcgg gccagctcct ccggcacacc ggaaacctcg aacatcacgc
     6241 ggccgggctt gaccacggca acccaggctt ccgggttgcc cttgcccttg cccatgcggg
     6301 tttcggccgg cttcttggtg atcggcttgt ccgggaagac gttgatccag accttgccgc
     6361 cacgcttcat ctttcgggtc atcgcaacac gggcagcttc gatctgacgg ttggtgatcc
     6421 agccgcgatc gagggacttg agcccgtact cgccgaaagt cacggcagtt ccgcccttgg
     6481 aattaccctt tcggcggccc cgcatgacct tgcggtgctt gacacgcttc ggcatcaaca
     6541 tcagcgacgg cctcccctgc gatcgtcgcc acggccgccg cggcggtcgg gacggtcgct
     6601 catcggagcc tcatccgagg tccgcgagga ttcgaagcct tcgggcatga cttcgccctt
     6661 gttgatccag cacttgacgc cgatgcgccc ggtgccggtc ttcgcctcga cgaagccgta
     6721 gtcgatgtcg gcgcgcaggg tgtggagcgg cacgcggccg tccgagtaac cctcggtgcg
     6781 ggccatttcg gcgccaccca ggcgaccgga aatctggacc ttgacgccct tggcgccgga
     6841 gcgcatggcg gaggtgagcg cccgcttcat cgcgcgccgg aaggcgacgc ggttctggag
     6901 ctgctcggcg atcgactggg cgaccagctt ggcgtcgagc tcggggcgct tgacctcgcg
     6961 gatgttcacc ttcaccggcg agccggtgat cttgtgaagg tccttgcgga gcgcgtcgac
     7021 ctcgctgccc gacttgccga tcacgatgcc cggacgggcg gtgtgaatgt ccacggcgac
     7081 ctcgccgcga cgctcgatcg tgatatcgga caggccggcg tgagcgagct tgttctcgat
     7141 gtggtcgcgg atcgccaggt cctgcatcag caggtccgaa aagtccttgt cggcgaacca
     7201 gttggacttc cagtcgtgga tgtagccgac tcggaagcct tcgggatgaa tcttctgtcc
     7261 catgcttcta ttcctcgtct tccggtgtca gcgccacgga gatgtggcag gtgcgcttgt
     7321 ggatcggggt ggcccggccc atcgcccgcg gccggaaccg cttcagggtg ggtccttcgt
     7381 cgacccggat ctcggcgacg atcatctcgt ccccgatcag gtcgtggttg gcctcggcgt
     7441 tggcggcggc cgactcgatc accttggaga tgtcgtcagc aaccgaacgc ggcgaaaact
     7501 gcagcagcga gcgggcgtcg tcgatgtgct tgccacggac ctggtcggcg accaggcggg
     7561 ccttgcgggg tgacacgcgc acgtacttcg aggtggcacg gaccagtacc ggctcggccg
     7621 cctcttcctt cctggtggtc ttggcagcca tcagtcctta ccgccggtgt gggacttgaa
     7681 ggtgcgggtc ggggcgaact cgcccagctt gtgaccgacc atcgattcgg agatgaagac
     7741 aggcacgtgc ttgcgaccgt cgtggacggc gatcgtgtgg ccgaccatct ccgggaagat
     7801 cgtcgaacga cgcgaccagg tcttgaccat gttcttgttg ccggcctcgt tcatggcttc
     7861 gatccggccc ataagccgct cttccacgta cgggcccttc ttggttgacc tacccacggt
     7921 ctacctaccc ttcttctttc cgcgacgacg gccgcgcacg atgtaacggt cggactgctt
     7981 gcccttcttg cgggtgcggt acccgagcgt gggcttgccc cagggagtga ccggatggcc
     8041 accgggggtc ttgtgtgcct cgccaccgcc atgcgggtgg tcgaccgggt tcatggcgat
     8101 gccgcgcgac tgcgggcgct tgcccttgtg ccggttgcgg ccggccttgc cgatcttgac
     8161 gttctgatgc tccgcgttcg aaatcgtgcc gatcgtcgcg cggcattccg cacggaccat
     8221 ccgcatttcc gaggaaggaa ggcgcagggt gaccatgtca ccctccttcg cgaccagctg
     8281 gatcgcggtg ccggcgctgc ggccgagctt gccgccctgg ccgggcttca gctccacgtt
     8341 gtggacggtg gtaccgaccg gcatctcgcc gagggcgagg cagttgccgg gacggatgtc
     8401 cgagcccggg ccggagctga cctggttgcc gacctcgatc tgcgagggcg ccaggatgta
     8461 ggccttctcg ccatccgcgt aatgcagcag ggcgatgtag gcggagcggt tcgggtcgta
     8521 ctcgacggtc gcgaccttgg cggggatccc gtccttggtc cgcttgaagt cgatcttccg
     8581 gtagcggcgc ttggcccctc cgccacggtg gcgggaggtg atgcggccgt tgttgttgcg
     8641 gccacccgac ttcttgatgc cctcgatcag gctcttctcg ggctcggtcg cggtgatctg
     8701 ctcacgcagc tggtacgtcg cgaagcgacg cccggggctc gttggttttg tcttgcgaat
     8761 tgccattact cagttattcc ttcaagccct tactcgaccg ccgcgccttc gaacagctcg
     8821 atgctgtcgc cgggggcgag ttcgacgatt gccttcttcc aggagcggct gcgaccctga
     8881 tgcaggccac gacgcttcgg ctttgaacgg acctttgagg tccggacctt gaccacttcg
     8941 actccgaacg ccttctcgac ggcgtccttg atctcgaact tgtgggcccg gtcgtccacc
     9001 cggaaggtgt acttgttggc cgcgatcagg gcgtaggcct tttccgagat gaccggacgg
     9061 atgatcacgc tgcggggatc catcactcac cgtccttttc ggtcccggtt ccccggtccg
     9121 gttcggtcga ctgggcggcc agaacctcga gggccgcttc ggaaaccacg atcgaagcgg
     9181 cgccgatcac gtcgacgact ccggtcgcgc caacctccat cacggagacc cgggggatgt
     9241 tgcggaagct cttcagcagg ccggtctctt ccccgaggat gatcaccagg gtcgagcgct
     9301 tcgcgcccca cttctcgagg gccgccgcgg cgtccctggt cgaaggggtc tcgaagtcgg
     9361 ccgccttgac cacggcgacg ctgccacgct cggcgtgaac cgagagggcg gagcgcatcg
     9421 ccttgcggcg agccttgcgg ttgaccttga ccgtgtaatg gcgcggcttc ggnnnnnnnn
     9481 nnccgccgcc gtagcggttc ggcacgctga gggcgccgac ccgggcacgg ccggtgccct
     9541 tctggcgcca ggccttggcg gtggtcattg aaacttcgcc gcgggtcagc gtcgaggcag
     9601 tgccctggcg ccgcgcgttg gcgtcggccc gggcggtctc gtggaccagc gactgatgga
     9661 aggcctcgtc gaagaggccg gccggcagat cggccttggt ggttttgccc agaacggggg
     9721 ccttggcgct agccatcggt acggatctcc acaatcgagt tcttggctcc gggaacggag
     9781 cccttgatca tcagcaggtt cctgtcggca tcgatgccgg cgatctccag gccgagctgg
     9841 gtgcggcgct tgccgcccat ctggccgggg aggcgcatgc ccttgaacac gcgggcaggc
     9901 caggcggacg caccgatcga gcccggggca cgaacgttgt gcgaaccatg cgaaacggga
     9961 ccgcggccga agttgtgccg cttgacggtg ccctggaagc ccttgccgat cgtgatgccg
    10021 gcgaccttga ccttctggcc ttcctcgaag gcctcgacgg tgacctgctg gccgagcttg
    10081 acctcgccgc cgtcaccttc ggcttcttcg ccttcggaac cttcttcgcc ggccgagcca
    10141 gcggcgggga gctccacgtc gcggaactcg accagcaccc tcatcgggtt ggcgtcggcc
    10201 ttggccaggt ggcccacttc ggccttgctg agcttcgact cagcttggcc aggtggccca
    10261 cttcggcctt gctcagcttc gactccttga cttcgtcgaa agcgagctga atcgccgagt
    10321 agccgtcgcg ctcctcgttg cggatcgcgg taaccggaca gggtccggct tcaatgactg
    10381 ttacgggcac gacctcaccg tcttctctga acagttgggt catgccaagc ttttttccga
    10441 gaatcgccga catggtgcct acagtcggat ctcgatgtcg acgccggccg ggagcgagtc
    10501 caggcgctgc agcgaatcga cggtcttggg ggtgggctgc ttgatgtcga tcagacgctt
    10561 gtgggtgcgg atctcgaagt gttcccgaga gtccttgtcc ttgaacggcg aacggatcac
    10621 gcagtagatg ttgcgctcgg tcggcagcgg cacgggaccg gaaacggtcg cgccggtgcg
    10681 ctcggccgta tcgacgatct cgcgggcagc gcgatcgatg gcgtcgtgat cgtaggcctt
    10741 gagcctgatc ctgattttct gtgcggcaat agcggccact ttggcttctc ttcttacttt
    10801 cttctgctgg cgcaaatcag ctgtatcggc tgaggcgctg atgtcaaggt cccccgccgc
    10861 ctgcggctca gtccccgccc gtgaggcgga tccagtgccg tggttcgaca atccggggta
    10921 cttcaaaggt cgtatctgtt actggtgctg cgaacttctt tacttcgtta ctgcgttact
    10981 gctgtgctgc ggtacttcag gccctgaggg cgcaaaaacg gggcggaccc gaaaacgttt
    11041 gctgtcttcg ggtccgcccc gggcaaatcg tgtcgctact cgatgatctc cgagacgacg
    11101 ccggagccga ccgtgcggcc accctcgcgg atcgcgaagc ggaggccctg gtccatggcg
    11161 atcggctgga tcagctcgat cgtcatctcg acgttgtcac ccggcatgac catctccgtg
    11221 ccctcgggca ggttggcgac gccggtcacg tcggtggtcc ggaagtagaa ctgcgggcgg
    11281 tagcccgaga agaacggggt gtgacgaccg ccttcttcct tcttcaggca gtaaacctcg
    11341 gcccggaact tggtgtgcgg ggtgatcgag cccggcttgc agagaacctg gccgcgctcg
    11401 atttcctcac gcttggtgcc acggagcagg cagccgacgt tgtcgccagc gcgaccctcg
    11461 tcgaggatct tgcggaacat ctcgacgccg gtgatcgtgg tggtctcggt cttgtcgtga
    11521 ataccgacga tctcgacctc gtcaccggtg ttgacgatgc cctgctcgat acggccggtg
    11581 gcgacggtgc cgcgaccggt gatcgagaag atgtcttcga tcggcatcag gaacggcttg
    11641 tcgaggtcac gctccggctc cgggatgtag gagtccagcg cctcgcccag ctcgacgatc
    11701 ctggcctgga agtcagcgtc gccttcgagg gccttgagag ccgaaccctt gatgatcggg
    11761 gtgttgtcac cgtcgaactc gtactcgttg aggaggtcgc ggacctcttc ctcgaccagc
    11821 tcgatgagct cctcgtcgtc gacctggtcg accttgttga ggaacacgac gacgtgcggc
    11881 acgttgacct ggcgggcaag caggatgtgc tcgcgggtct gcggcatgac accgtcagcg
    11941 gcagaaacga ccaggatcgc gccgtccatc tgggcggcac cggtgatcat gttcttgacg
    12001 tagtcggcgt ggc
//
LOCUS       DAOQKN010000066        11852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_2529_length_11852_cov_5.487921, whole genome shotgun sequence.
ACCESSION   DAOQKN010000066 DAOQKN010000000
VERSION     DAOQKN010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 11852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11852
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2529_length_11852_cov_5.487921"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /locus_tag="PLJ64_06100"
     CDS             <1..999
                     /locus_tag="PLJ64_06100"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNC93088.1"
                     /translation="YPGVERVRFTSSGTEATMDAVHLARGATGRDMILKIEGSYHGHH
                     DAVMVSVAPPLDQIGTREDPVAVPYGEGTPKALTDLTHTVPFNNVDALKAVLENIGDQ
                     VAGMIVEPAMMNINIIPPKDGYLQAVRELTEQYGIKLIFDEVKTGATMSHGGATGYYG
                     VTPDMITLAKATCGGYPGGSIGMSDEVAQLVEDDRVKQYGTFNGNPLVMAAAKAALTE
                     VLTEQAYVELNEKNEYLKGRCQEIIDKYGLPAYTEGIGAKGCVIFSPERLYEYRDYAE
                     YVDAELSTLGWLYHMNNGIFMTPGVEEEWTLSVFHTEQDLDNYANAFEAFAQDVTA"
     gene            complement(1053..1853)
                     /locus_tag="PLJ64_06105"
     CDS             complement(1053..1853)
                     /locus_tag="PLJ64_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93089.1"
                     /translation="MGMEASIFSRFWHDQIETNHQEAIFLLFVGFLLSFAFIRMSTRL
                     MRSPKVPWWPGSIVSEGGLHVHHHVFGIVLMMISGAIAFALDGGGTAFNLCGLAFGVG
                     IGLTIDEFALWLHLEDVYWSEEGRRSIDATVLTALVLALLMFGVRPFEIGSDSIGVLI
                     GSIITALFVFLVVAICFLKERVMHAFVGFFVWPVALYGACRLGKPGSPWAKRRYSERN
                     PSKQKRAEARFRPGRRTDRFKEKFRDLVGGGLIHPETDSPDDGSGTDT"
     gene            1911..3944
                     /locus_tag="PLJ64_06110"
     CDS             1911..3944
                     /locus_tag="PLJ64_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNC93090.1"
                     /translation="MSNRLAEETSPYLLQHKDNPVAWQPWGPGALAEAQEREVPILLS
                     VGYAACHWCHVMERESFEDEKTAAYMNEHFVPVKVDREERPDIDSIYMEAVQSITGQG
                     GWPLTAFLDPQGVPFHGGTYFPPDTSRGMPSFMMVMEAVIKAWDEKRDEILAQSGDVR
                     SRLGAVSLIGAPEGDLDPELPKRRAANLAEQADMDLGGFGPAPKFPPASALDFMLGQG
                     ETAAAGVTLDRMAAGGIFDQLAGGFARYSVDDRWLVPHFEKMLYDNALLARTYLRAWQ
                     TTGRDRYRWVAQKTLDWAMAEMLDPAGGFYASLDADSEGHEGLFYTWTPAEIEEALGQ
                     DAGEAIEAFGVTAEGHLEGRSVLHLPGGAPPDEIDPGLEELTDRLLAARSARIRPDLD
                     DKVLSSWNSLMISALSEAGAVLGRTDYLEAAQRCADFIWNGMRSPQGNLLRTWRQGEA
                     RLNAYLEDHAFLLEALLDLYQACFDPVVFDRARAVAETMIDRFSDPARGGFFTTSHDH
                     EELIARRKDVGDHPIPASSSSAALGLLRLAAMTGESAYRRKAEEVLKLLAPATSRQPA
                     AFGHLLQALAFYHSGGAELAIAWPADADAEADRPGCAVPAAAEPLVAEARRRYRPNLV
                     IAGGPDGSSTPPLMQDRTSLEGQPAAYVCQSFTCQAPVNDPAELGKELDALDQ"
     gene            3928..4425
                     /locus_tag="PLJ64_06115"
     CDS             3928..4425
                     /locus_tag="PLJ64_06115"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HNC93091.1"
                     /translation="MPSTSEIATHYFECVSRQDIPGMVACWKPGSRATFVGMDELNAP
                     EDYDQWFGNLFGCFPDFKLEAQSIVAEGEQASVHWRATGTFNGEGIFEGFRPNGASID
                     IEGVDLVTLEDGKIVHITAILNGLDMARQLGAVPAKGSIADKAMAAAFNTKTATIRKL
                     KSLRN"
     gene            complement(4437..5789)
                     /locus_tag="PLJ64_06120"
     CDS             complement(4437..5789)
                     /locus_tag="PLJ64_06120"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HNC93092.1"
                     /translation="MAVQTNTPEAVFQAVRENDVRFVRLWFTDVLGNLKSFSINSSEL
                     ERAFEQGIGFDGASLTGFNPVEESDMVARPDASTFSLIPWRPGGTDAVARMFCDIQVP
                     GGLAYEGDPRWVLKRALERAADLGFDDFVVAPELEFFYFRSARPEGGVPEVLDEGGYF
                     DLTTLDAGSDVRRETVLALEQMGVHVASTHHEAGPSQHELDLKPAGALATADDVMTSK
                     IVVKEYAVQQGWHATFMPKPIGGANGSGMHLHQSLTRDGANAFYNGEKPGALSPTGKA
                     FIAGQLRHAREIAPLYAQWVNSYKRLVPGFEAPVYLSWSRRNRSSLIRVPQQNGEEPD
                     GAKVEIRCPDPACNPYLCLAGLLQAGLEGIEKGYELPEPMEENLYHLGPDDLAQRGIE
                     QLPATLGEAIELAADSELVLRTLGEHTFRRFIELKRQEWDSYRVQVTPFEVESFLPVL
                     "
     gene            6003..6569
                     /locus_tag="PLJ64_06125"
     CDS             6003..6569
                     /locus_tag="PLJ64_06125"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNC93093.1"
                     /translation="MTVKTEVSRFHIHDELTAPDESAPILKSIQVGGRQVNKLVGVLA
                     GSPAVLRAYTRMRAELQQGELPQATRQRIALAVAEHRGDAYSVVQYAKSARNAGLGLD
                     EVSRARSFTSSDPKEQALLTYLKAALEADGDPPIYLHEEAREAGWDDEEILEALAHVA
                     MGEFQSVMAAAAALPKDQATPRVLPDAA"
     gene            complement(6713..7516)
                     /locus_tag="PLJ64_06130"
     CDS             complement(6713..7516)
                     /locus_tag="PLJ64_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93094.1"
                     /translation="MSTKTFPTVGRRTQAAAAFAGLIALIAGLAFMSGPGNQAGAASG
                     AAAAAGKAVYLGKASAKRIPLCPQNCQSVVIVSGFQAKAGGVGNAYRVPFIGNITRWR
                     LKLGKPTAKDRQFFQDRFGAVPQAAIGVLAKREQNGKIVYKLRRRSAIQNLNRFLGKT
                     ATFNLAAPIKVNKGDYVALIVPTWAPALAVPDACSIVRVNGKDQMKNPSVCRQFNASN
                     SWVASRDRTTCTQKVTMRNSQPQIDVNSLASYGCRFNGALTYGVRVESR"
     gene            complement(7685..8380)
                     /locus_tag="PLJ64_06135"
     CDS             complement(7685..8380)
                     /locus_tag="PLJ64_06135"
                     /inference="COORDINATES: protein motif:HMM:NF015298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlpA-like double-psi beta-barrel
                     domain-containing protein"
                     /protein_id="HNC93095.1"
                     /translation="MERSHRLGKLNIAVVAGILLVLALVVVLAGSGNDKAAASTGGIG
                     LASSKKSQARTSSSLVRGARLGARTLREGMSGPDVKVLNSIVRFKNFLSGAATPTFSR
                     PTTSAVRRFQNSADMASSGVVNKTTARALIGSLHTSEASWYGPGLYGNGTACGKVTLR
                     PTTIGLANKNLPCGTRVLIGYRGKFIIAPVIDRGPYGPGRTWDLTKAAADAVGLTSAG
                     VGRVRHAILSRGY"
     regulatory      complement(8387..8540)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            8781..9174
                     /gene="rnpB"
                     /locus_tag="PLJ64_06140"
     ncRNA           8781..9174
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLJ64_06140"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(9638..10315)
                     /gene="lexA"
                     /locus_tag="PLJ64_06145"
     CDS             complement(9638..10315)
                     /gene="lexA"
                     /locus_tag="PLJ64_06145"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HNC93096.1"
                     /translation="MVDLTKRQREIFDYIGKYTDKMGYPPTVREIGQAVGLTSSSTVH
                     VHLANLEKAGVLRRDPSKPRALEVLVGKAKSAGGSVRDSARDVVDSVATAASNVAAAP
                     SGLPLLGRVSAGPGILAEENIEDYVDVPGMIGGSDGDYVLEVRGESMKNAGIMDGDYV
                     IVHPSPEARDGEIVVALLGDEDATVKRFYKETDHFRLEPENETMSSIITTDAQVMGKV
                     IGVFRKV"
     gene            10519..10602
                     /locus_tag="PLJ64_06150"
     tRNA            10519..10602
                     /locus_tag="PLJ64_06150"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10553..10555,aa:Leu,seq:gag)
     gene            10688..11131
                     /locus_tag="PLJ64_06155"
     CDS             10688..11131
                     /locus_tag="PLJ64_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNC93097.1"
                     /translation="MAIKRMDNVLIVVEDMAAVKDFFIELGMEFDGETTVEGPWVDQT
                     VGLEGVRADITMLRTPDGGCRVELSRFHNPPAVRAEPEDAPSNALGIRRIMFAVDDID
                     EVVERLVSRHEAKLVGEIVRYGDSYRLCFLRGPEGIVVGIAEELG"
     gene            11196..11816
                     /locus_tag="PLJ64_06160"
     CDS             11196..11816
                     /locus_tag="PLJ64_06160"
                     /inference="COORDINATES: protein motif:HMM:NF013152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HNC93098.1"
                     /translation="MRRLLPLILGLALLWAPAQAGAVTAQTARFVAGFEGFVSCPYAD
                     PAGHATIGYGHLLHYGPPTRADRRKWGCLTQAQALRLLRQDLRETEIEVFDRIRGAKV
                     NAPMVTALTSFAFNLGAGYLDFVAKRGSRPATNIGRAIRVGKYRRAARQMLYFDGVIV
                     GGKRYELEGLQIRRRKEYRLMIRGIHQLRACDGACSDGPDGGLGVN"
BASE COUNT         2127 a         3843 c         3912 g         1970 t
ORIGIN      
        1 taccccggtg tcgagcgcgt gcggttcacg tcgtcgggga ccgaagcgac catggacgcc
       61 gtccacctag cccgcggcgc caccggccgc gacatgatcc tcaagatcga gggctcctac
      121 cacggccacc acgacgcggt gatggtctcg gtcgccccgc ccctggacca gatcggcacc
      181 cgcgaggatc cagtcgccgt tccctacggc gaaggcaccc cgaaggcgct gaccgacctc
      241 acccacaccg tccccttcaa caacgttgac gcgctgaagg ccgtgctcga gaacatcggc
      301 gaccaggtcg ccgggatgat cgtcgagccg gcgatgatga acatcaacat catcccgccg
      361 aaggacggtt acctccaggc cgttcgcgag ctgaccgagc agtacgggat caagctgatc
      421 ttcgacgagg tcaagaccgg cgcgaccatg tcacacggcg gcgccaccgg ctactacggc
      481 gtgaccccgg acatgatcac cctcgccaag gcgacgtgcg gcggttaccc gggcggctcg
      541 atcggcatgt cggacgaggt cgcccagctg gtcgaggacg accgcgtgaa gcagtacggc
      601 accttcaacg gcaacccact cgtcatggca gccgcgaagg ccgccctgac cgaggtcctg
      661 accgagcagg cctacgtcga gctgaacgag aagaacgagt acctcaaggg ccgctgccag
      721 gagatcatcg acaagtacgg cctgcctgcg tacaccgagg gcatcggcgc caagggctgc
      781 gtgatcttct cgcccgagcg gctctatgag taccgcgact acgccgagta cgtggacgcc
      841 gagctctcaa ccctgggctg gctctaccac atgaacaacg gcatcttcat gactcccggc
      901 gtcgaggaag agtggaccct ttcggtcttc cacaccgagc aggacctcga caactacgcg
      961 aacgcgttcg aggcgttcgc gcaggacgtc accgcctgat caggcgcggc cggcggtcga
     1021 ttgatcgccg gccggccagc ctccggccgt cttcaggtgt cggtacccga cccgtcgtcc
     1081 ggggagtcgg tttcagggtg gatcagcccg ccgccgacca gatcgcggaa cttctccttg
     1141 aaccggtcgg tgcggcggcc ggggcgaaag cgcgcctcgg cccgcttctg ctttgaggga
     1201 ttcctttccg agtagcggcg tttcgcccac ggcgagccgg gcttgcccag ccggcaggcg
     1261 ccgtaaaggg cgaccggcca gacgaagaac ccgacgaagg catgcatcac cctctccttg
     1321 aggaagcaga tcgcaaccac aagaaagacg aaaagggcgg tgatgatgct gccgatcagg
     1381 acgccgatcg agtccgaacc gatctcgaaa ggtcggaccc cgaacatgag cagcgccagg
     1441 accagcgcag tcaggacagt cgcgtcgatc gagcgacgac cctcttcgga ccagtagacg
     1501 tcctcgaggt gaagccagag cgcgaactcg tcgatggtca gaccgatgcc gaccccgaag
     1561 gcgaggccac agaggttgaa tgcggtgcct cccccgtcga gcgcgaaggc gatcgccccc
     1621 gagatcatca tcaggacgat gccgaagacg tggtgatgaa cgtgtagccc gccctcgctg
     1681 acgatgctgc ccggccacca cggaaccttc ggggatcgca tcaaccgcgt gctcatccgg
     1741 atgaaggcga aggaaagcag gaagcccacg aacagcagga agatcgcttc ctgatggttg
     1801 gtctcgatct ggtcatgcca gaacctgctg aagatcgacg cctccattcc catcgccccc
     1861 caatcctgcc acgaccaacg cccgcatgtc cgaaccccca tcatgtgtca atgagcaacc
     1921 gccttgccga agagaccagc ccgtacctcc tccagcacaa ggacaacccg gtcgcgtggc
     1981 agccctgggg tcccggggcg ctggccgagg cgcaggaaag ggaggtgccg atcctgcttt
     2041 cggtcggata tgccgcctgc cactggtgcc acgtgatgga gcgggaatcc ttcgaggacg
     2101 agaagaccgc ggcctacatg aacgagcact tcgtgccggt caaggtcgac cgggaggagc
     2161 ggccggacat cgactcgatc tacatggagg cggtccagtc gatcaccggt cagggcggct
     2221 ggccgcttac cgccttcctc gacccgcaag gagtgccatt tcacggcggg acctactttc
     2281 cacccgacac ttcccgcggc atgccgagct tcatgatggt gatggaggcg gtgatcaagg
     2341 cctgggacga gaagcgggac gagatcctcg cccagtccgg cgatgttcgc tcgaggctcg
     2401 gggcggtgtc gctgatcggc gccccggaag gagatctcga ccccgaactg ccgaagcgcc
     2461 gggccgcaaa cctggccgag caggcggaca tggacctcgg cggattcggg ccggccccga
     2521 agtttccgcc ggcctccgcg ctcgacttca tgctgggtca gggcgagacg gcagccgccg
     2581 gggtgaccct cgaccggatg gcggccggcg gcatcttcga ccagctcgcc ggcggcttcg
     2641 cccgctactc ggtcgatgac cggtggctgg ttccccactt cgaaaagatg ctttacgaca
     2701 acgccctgct ggcccggacc tatctgaggg cctggcagac aaccggtcgc gaccggtacc
     2761 gctgggtggc ccagaagacg ctcgactggg cgatggccga gatgctcgac ccggccggcg
     2821 gcttctacgc ctcgctcgac gccgattccg agggtcacga gggacttttc tacacctgga
     2881 ccccggccga gatagaggag gcgctcggtc aagacgcggg ggaagcgatc gaagcgttcg
     2941 gggtcacggc cgaaggccac ctcgaaggtc gcagcgtcct ccatctcccg ggtggcgccc
     3001 ctcccgacga gatcgatccg ggtctcgagg aactgaccga ccgactgctc gcggcccgct
     3061 cggcgaggat ccggccggac ctcgacgaca aggtgctcag ttcctggaac tcgctgatga
     3121 tctcggccct gtccgaggcc ggcgcggtgc tcggacggac cgactatctc gaagccgccc
     3181 agcgctgtgc cgacttcatc tggaacggga tgcggagccc gcagggcaac ctgctccgga
     3241 cctggcgtca gggcgaagcg agactgaacg cctacctcga agaccatgcc ttcctgctcg
     3301 aagccctgct cgacctgtac caggcctgct tcgatcccgt cgtcttcgat cgggctaggg
     3361 cggtcgcgga aacgatgatc gatcgcttct cggacccggc gcgtggcggc ttcttcacca
     3421 cctcgcacga ccatgaggag ctgatcgccc gccgcaagga cgtcggagac cacccgatcc
     3481 cggcttcttc ctcctcggcg gcgctcgggt tgctccgcct cgcggccatg accggtgaat
     3541 cggcatatcg acgcaaggcc gaggaggtac taaagctgct ggccccggcc acctcccgcc
     3601 agccggcggc gttcggccat ctgctccagg ccctggcctt ctaccactcg ggaggcgccg
     3661 aactggcgat cgcctggccg gcggatgcgg acgcggaagc ggatagaccg ggttgcgctg
     3721 ttcccgcggc ggcggagccg ctggtggcgg aggcccgccg gcgctaccgt ccgaacctgg
     3781 tgatcgccgg cggaccggat ggatcatcga ccccgccgct gatgcaggac cggacttcac
     3841 tcgaaggcca gccagccgca tatgtttgtc agagcttcac ctgtcaggcg ccagtcaacg
     3901 accccgcgga gcttggaaag gaactcgatg ccctcgacca gtgaaatcgc cacccactac
     3961 ttcgaatgtg tgagccgcca ggacattccc ggcatggtcg cctgctggaa gccgggcagc
     4021 cgcgccacct tcgtcgggat ggacgaactc aacgcccccg aggactacga ccagtggttc
     4081 ggcaacctgt tcggctgctt ccctgacttc aaactggagg cgcagagcat cgtcgccgag
     4141 ggcgagcagg cctcggtcca ctggcgggcg accggcacct tcaacggaga aggcattttc
     4201 gaaggctttc gaccgaacgg ggcgagcatc gacatcgagg gggttgatct cgtcaccctg
     4261 gaggacggga agatcgtcca catcacggcc attctcaacg gcctggacat ggcccgccag
     4321 ctcggcgcgg taccagcaaa gggttccatc gccgacaagg caatggccgc cgccttcaac
     4381 acgaagaccg ccacgatcag gaagctgaag agcctgagga actgaccgga gccgggctaa
     4441 aggaccggca ggaagctttc gacctcgaag ggggttacct ggacgcggta ggaatcccat
     4501 tcctggcgct tcagttcgat gaagcggcgg aaggtgtgtt cgcccagggt gcgcaggacc
     4561 agttccgagt cggcggccag ttcgatcgct tcgccgagtg tggccggcag ctgttcgatg
     4621 ccgcgctggg caaggtcgtc ggggccgagg tggtagaggt tctcctccat cggctcgggc
     4681 agttcgtagc ccttttcgat cccttcgaga ccggcctgaa gcagcccggc gaggcaaagg
     4741 taggggttgc aggcagggtc ggggcagcgg atctcgacct tcgctccgtc cggctcctcg
     4801 ccgttctgct ggggaacccg gatcagcgag gaacggttgc ggcgcgacca ggaaaggtag
     4861 accggggcct cgaagccggg caccagccgc ttgtacgagt tgacccactg cgcgtagagc
     4921 ggcgcgatct cgcgggcgtg ccggagctga ccggcgatga acgccttgcc ggtcggtgaa
     4981 agggcgccgg gcttctcccc gttgtagaag gcgttggcac cgtccctggt cagggactga
     5041 tgaaggtgca tcccggaacc gttggcgcca ccgatcggct tcggcatgaa ggtcgcgtgc
     5101 cagccctgct ggaccgcgta ctccttgacc acgatcttcg aggtcatcac gtcatccgca
     5161 gtggccagcg ccccggccgg cttgaggtcg agttcgtgct gggaaggccc ggcctcgtgg
     5221 tgggtcgaag caacgtggac ccccatctgc tccagcgcca ggaccgtctc gcgccgcacg
     5281 tcggagccgg cgtcaagggt ggtcaggtcg aagtagccgc cctcatcgag aacctctggc
     5341 acaccgcctt ccgggcgggc ggaccggaaa tagaagaact ccagttcggg ggcgaccaca
     5401 aagtcgtcga aacctaggtc cgcggcccgc tccagggccc gcttcagaac ccagcggggg
     5461 tcaccttcgt aggcgagacc gcccggtacc tggatgtcgc agaacatgcg ggcgaccgcg
     5521 tcggtcccgc cgggtcgcca gggaatgagg ctgaaggtcg aagcgtcggg ccgggcgacc
     5581 atgtccgatt cctcgaccgg attgaaacct gtcagcgagg ccccgtcgaa gccgatcccc
     5641 tgctcgaagg cccgttcgag ttcgctcgaa ttgatcgaga agctcttcag gttgcccagc
     5701 acgtcggtga accagaggcg gacaaaacgg acgtcgtttt ccctgactgc ttgaaagacg
     5761 gcttccgggg tatttgtctg tactgccatc ggactccttg gaggggctaa ataagcggct
     5821 tcgagccgga atcgaggtgc ttcaggcgga aacgatacgc gaggggcttg cgtaacgccg
     5881 ccggagtggg tataacttac ttgcggtaag taactagcta aaagtaaagt aatggctcgt
     5941 taaacggacg gtaaaggccg gggacgagcc gtaactcgga gaacactggg aagcgtgaca
     6001 tcgtgaccgt aaagacagaa gtcagccgat tccatatcca cgacgaactg accgctcccg
     6061 acgagagcgc gccaattctc aagagcatcc aggtgggcgg ccgccaggtg aacaagctgg
     6121 tcggcgtgct ggccggctcc cccgccgtgc ttcgcgccta cacccgcatg cgtgcggaac
     6181 tccagcaggg tgagcttccc caggccaccc ggcagcggat cgctctcgcg gtggccgaac
     6241 acaggggtga tgcctacagc gtcgtccagt acgcgaagtc tgcccgcaac gccggcctcg
     6301 gcctcgatga agtgtcgcgg gcccgcagct tcacttcctc ggacccgaag gagcaggcgc
     6361 tgctcaccta cctgaaggcc gcgctcgaag cagacggcga cccgccgatc taccttcacg
     6421 aggaagcccg cgaggccggt tgggatgacg aagagattct cgaagccctg gctcacgtgg
     6481 caatgggtga gttccaaagc gtgatggctg cagccgcagc cctcccgaaa gatcaggcaa
     6541 ctccgcgggt tctgcccgac gccgcctgat cacctccata aaaggaccct cccccggggt
     6601 ccgatcgagg cggcccctct ctctcccctg gccgacctct ccccctaaaa aaaaggacgg
     6661 ccccgatcgg ggccgtcctt tctttcgtac ctctcgaaac tgtgtcgaag cgctagcggc
     6721 tctcgacccg gacgccgtat gtcagggcgc cgttgaaccg gcaaccgtat gacgccaggc
     6781 tgttgacgtc gatctgcggc tgcgaattcc tcatcgtgac tttctgggtg caggtcgtgc
     6841 gatcgcggct ggcgacccag ctgttgctcg cgttgaactg cctgcagacg ctcgggttct
     6901 tcatctggtc cttgccgttc acccggacga tcgagcaggc gtcgggaacg gcgagggccg
     6961 gagcccaggt cgggacgatc agggcgacgt aatcaccctt gttcaccttg atcggggcgg
     7021 ccaggttgaa ggtcgcggtc ttgcccagga accggttcag gttctggatc gccgagcggc
     7081 gacggagctt gtagacgatc ttgccgttct gctcacgctt cgcgagcacg ccgatcgcag
     7141 cctgtggaac ggcgccgaac cggtcctgga aaaactggcg gtccttggcg gtgggcttgc
     7201 cgagcttcag gcgccagcgg gtgatgttgc cgatgaaggg gacccggtaa gcgttcccga
     7261 cgccaccggc cttcgcctgg aaaccactga cgatgaccac ggactggcaa ttctgcgggc
     7321 aaaggggaat gcgcttggcg cttgccttgc cgagatagac cgccttgcca gcggcggcag
     7381 cggcaccgct ggctgcgccg gcctggttgc cggggccgct catgaaagcc aggccggcga
     7441 tcagggcgat caggcccgca aacgcggcgg cggcctgcgt gcggcggccg acggtcggga
     7501 aggtcttcgt tgacatcagt ttcactagct ccttgttgta agtctcgcgg cgcaagtcag
     7561 gtatttggcg cggcgggtca cggtacaaac agtcgaaaaa ccgcgatgga tcggtttttc
     7621 cctgcaaaga aaaggtcgga agggggtttc gccccccgcc gacgcctgat ttccggtgat
     7681 gcggttagta gccgcggctg agaatcgcgt ggcggacccg gccgacgccg gcgctggtca
     7741 ggcctaccgc atcggcagca gccttggtca gatcccaggt gcgtccgggc ccgtagggac
     7801 cacggtcgat gaccggcgcg atgatgaact tgccgcggta gccgatcagc accctggtgc
     7861 cgcaagggag gttcttgttg gcgaggccga tcgtggtcgg acgcagggtg accttgccgc
     7921 aggcggtccc gttgccgtag aggcccgggc cgtaccagga agcttcggaa gtatggagcg
     7981 acccgatcag cgcccgggcg gtcgtcttgt tgacaacccc gctgctggcc atgtcggccg
     8041 agttctggaa gcggcggact gcgctggtgg tggggcgact gaaggtcggg gtggccgcgc
     8101 cggagaggaa gttcttgaag cggacgatgc tgttcagcac cttcacgtcg gggccggaca
     8161 tgccctcgcg aagcgtccgg gcgccgagcc gggctcccct gaccaggctg gaagaagtcc
     8221 gggcctggga cttcttggaa gaagcgaggc cgatgccacc ggtcgaggct gctgccttgt
     8281 cgttgccgct gccggccaga accacgacca gggccagcac gagaagaatc ccggcgacga
     8341 ctgcgatatt cagtttgccc agccgatggc tgcgttccat tccttcttct ctctttcgta
     8401 aggcttgcgg ggttagctgt cgggctcgcg ctgaaagagc agcgctacct ggcggatgcc
     8461 agtgattcgc ccctggaccg atgtcctttg ggtcccccgt tctccgcgat cttgaggcgg
     8521 agattcagca gaaggttcgg gaagattagc aaacactcac gttatgtgtg catttcattt
     8581 gtgtgatgta cgtccgtttc ttgaaaacga ggcgcgagaa ctccgggcgg ggaccggagg
     8641 taccgcctcc tcggcccgaa acgtcgtcgg cgccacctcc gatccccgca aagtggcgcg
     8701 ccgatcagtg ccgcatgccg gatcgacatg ggcgctcccc cactttcagc ccgccgctac
     8761 caggccggcg gtacaattca tctgtgggtc ggacggctgc ggggcgagag aaaacgcttc
     8821 gaggaaagtc cggacaccga agggcaagga cggtgggtaa cgcccacccg gggaaacccg
     8881 cgggaaagtg ccacagaaat acacggccga tggcgcgacc acacgtgagt cgcgatcagg
     8941 caaaggtgaa aaggtgcggt aagagcgcac cggcgtcgtg gtgacacggc ggccaggtaa
     9001 accccgtccg gtgcaagacc aaacaggatc gtcgacccgg cccggcgaga tccaggtagg
     9061 tcgcaggcaa gtccgggatt cgctcagcga accccggcag gcccaccggt ccgatcaacc
     9121 ggtgagatag atggtcgttc aagacaggat ccggcttaca gacccactaa ggaagaggcc
     9181 ccctcggggg ccttttctac ttgcatcaaa gcttccaggt aacgccgctt tgcccaggtc
     9241 ggtggttacg ccgtggggcc gggtgggggt agccgggacg gacgcacgcg cggcattcag
     9301 ccgcgctggt cctggagtgt cccggagacc cccacccgac cccacggcta cacgaacccg
     9361 ggaggtcccc acccgacccc gcggctgcca tggccctccc gacgcgattc gcgagagtcc
     9421 acttttggcc gcatagcgca ctcaactccg atctcgaatc ggcgggccgg atcgaagtcg
     9481 gagttgggtg cgctatttgc cgataactcc cgcctcgaat tcccgggact ccgacgcact
     9541 cccccttcac cagcccccgt ggacttcaag gtgccgcagg tttgcgacct ctgcggttgc
     9601 aaacctgcgg cacctttcag agtcggaccg cccgggccta gacctttcgg aaaactccga
     9661 tcaccttgcc catgacctgg gcatcggtgg tgatgatcga gctcatcgtc tcgttctccg
     9721 gttcgagacg gaaatggtcc gtttccttgt agaagcgctt gaccgtggcg tcttcatcgc
     9781 cgagcagggc gaccacgatc tcgccgtcgc gggcttcagg tgaggggtgg acgatcacgt
     9841 agtcgccgtc catgatgccg gcgttcttca tgctttcgcc gcgaacctca agcacgtagt
     9901 cgccgtcgct gccgccgatc attccgggca cgtcaacgta atcctcgatg ttctcctcgg
     9961 cgaggatgcc gggaccggcg gaaacccgtc cgagcagggg caggccggaa ggcgcggcgg
    10021 ccacgttgct ggctgcggtg gcgacgctgt caaccacgtc acgggcgctg tcccggacgc
    10081 tgccgccggc gctcttcgcc ttgccgacca gcacctcgag ggcccggggc ttgctcgggt
    10141 cacgacgcag gactccggcc ttctcgaggt tggcgagatg cacgtggacg gtcgatgagg
    10201 aagtgaggcc gactgcctgg ccgatttccc ggaccgtggg cgggtaaccc atcttgtccg
    10261 tgtacttgcc tatgtagtcg aatatttcgc gctgccgctt ggtcagatca accacgacgc
    10321 tccttttcgg ctctcggcct tctgttggga agcggtccgg cccggagtgg cgaacctgtg
    10381 ttcgcaatgt acccctcctt gcggacgtat gttcgtgccg gggcgaaaac cgcaacctga
    10441 cgaccagaat gcgaccgaat cgaatccggg tcagcctgcc gccgcctgcc cccttcccct
    10501 agaatccgcg gtcctcttgc ggctgtggcg gaattggtag acgcgcaagc ttgaggggct
    10561 tgtgtccgca aggacgtgga ggttcgactc ctctcagccg catgcatccg gttccggcct
    10621 gccccacggg cgggccggtt ccgtctgcca tggttcgggt ccggcatccg gcaaggagag
    10681 gatcaaagtg gcaataaagc gaatggacaa cgtcctcatc gtggtcgagg acatggcggc
    10741 ggtaaaggac ttcttcatcg aactcggcat ggagttcgac ggggagacga cggtcgaggg
    10801 gccctgggtg gaccagaccg tggggcttga aggcgtccgg gcggacatca ccatgctgcg
    10861 gacgccggat ggaggctgcc gggtggagct ttcgagattt cacaatccgc cggcggtcag
    10921 ggccgaaccg gaagacgcgc cgtcaaacgc cctcgggatc cgccggatca tgtttgcggt
    10981 cgacgacatc gacgaggtgg tcgagcgtct ggtcagccgg catgaagcga agctggtcgg
    11041 tgagatcgtg cgctacgggg acagctaccg gctctgcttc ctgcggggac cggagggaat
    11101 agtggtcggg atcgccgaag aactcggctg aaagcggggt tccgcgcgtc tcctgtgccg
    11161 cgtcgcgggc gcccggactg ctatccattg aggcgatgcg tcgcctcctt cccctcattc
    11221 tgggcctggc cctgctctgg gccccggccc aggcgggcgc cgtcacggcg cagaccgcca
    11281 ggttcgtggc cggcttcgaa gggttcgttt cctgccccta cgccgacccg gccggccacg
    11341 ccacgatcgg ctacgggcac ctgctccatt acgggccgcc gacccgggcc gatcgccgca
    11401 agtggggctg cctgacccag gcgcaggccc tccggctgct gcgccaggac ctgagagaaa
    11461 ccgaaatcga ggtctttgac cggatccggg gagccaaggt gaacgcgccg atggtcacgg
    11521 cgctgacctc gttcgccttc aacctcggag cgggctacct tgacttcgtg gcgaagcggg
    11581 gcagccgtcc ggcgaccaac atcggccgcg cgatccgggt cggcaagtac cggcgcgcgg
    11641 cgcggcagat gctctacttc gacggcgtca tcgtcggcgg caaacggtac gagctcgagg
    11701 gccttcagat caggaggcgc aaggagtacc ggctgatgat tcgcggcatc caccagctga
    11761 gagcctgcga cggcgcctgc agcgacggcc cggacggcgg cctgggcgtc aactgaccgg
    11821 gtccggcagg cgccggctgg tcccgggcag cg
//
LOCUS       DAOQKN010000067        11799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_2545_length_11799_cov_5.872786, whole genome shotgun sequence.
ACCESSION   DAOQKN010000067 DAOQKN010000000
VERSION     DAOQKN010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 11799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11799
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2545_length_11799_cov_5.872786"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /locus_tag="PLJ64_06165"
     CDS             <1..815
                     /locus_tag="PLJ64_06165"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNC93099.1"
                     /translation="IEAFKRMGEVAEAGLPVEALLQLTRVYAQAVRRIADAEVRLFHL
                     FVHEPMIRDGLSEIEMAEELGDLARRTSPTAVPLFQYLHDRYLRYFLEQDVIGHMESD
                     FGGDTNLDQVRTTLCFIDLTGFTRFTEQEGDETAFSVVEQFTEAVEATLPPEAGIVKT
                     IGDEVMVVSPDPASLAEWAVGFLALFEDRPKPRAGIHHGAVVFRDGDYYGSQVNLAHR
                     VVNRALGGEVLITDAVAAAIEDHPDLETDAIGEVDLKGFPVPTPLFLVRARS"
     gene            916..2244
                     /gene="tilS"
                     /locus_tag="PLJ64_06170"
     CDS             916..2244
                     /gene="tilS"
                     /locus_tag="PLJ64_06170"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNC93100.1"
                     /translation="MLVSGGADSVALLLGLHLVLGAGQLVALHVNYGLRPTADDDERL
                     VRRICEQLEVELVVLRAGEAEGNMQAWARKIRLDEAERIRSERGLDWIAVGHNRSDQV
                     ETFIYRLVSSPGARSLLAMPPRSGNLIRPLLTLDRGLIRTMLERVTHSEDPTNDDPAF
                     ARNRIRLEVMPALEQVNCGAELNVVRTRAELAEDEEALMRLAVDAVALSGAAGPGGSR
                     GIPAKSLEDQPPAVRRRMIRHLAEAELDRPVAVSADLASEVLRLAGMPEGGRLDLGGG
                     DSLLAEAGRVRVCPGGGTGHDAVPEPARIDLKAGVAAFGGWEIESSREVEAEVRSAFG
                     DPWAAFLDLDDLLIWLIEKSPGPDDLLLQVRPWRFGDRIEPLGMSGSKSLQDVFTDAL
                     VPASRRQSWPVLVVGEKVIWVPGLVRSRHLLIAGPDRDVISLHARPPFSI"
     gene            2263..2826
                     /gene="hpt"
                     /locus_tag="PLJ64_06175"
     CDS             2263..2826
                     /gene="hpt"
                     /locus_tag="PLJ64_06175"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HNC93101.1"
                     /translation="MAATPPNELVSVGEVLITAEDLQRKVADLGAEVSRDYAGKDLVM
                     VGVLRGAVVFIADLLRHLTVPCEIDFMAVSSYGSSRDSSGVVRILKDLDDSITGRDVL
                     IVEDIVDSGLTLNYLMRNLQARDPASLEVCTLLTKPERRRVDVPIRYTGFEIPNQFVV
                     GYGLDFAQRYRNLDHVALLPEDAVPDR"
     gene            2957..4942
                     /gene="ftsH"
                     /locus_tag="PLJ64_06180"
     CDS             2957..4942
                     /gene="ftsH"
                     /locus_tag="PLJ64_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNC93102.1"
                     /translation="MKRFFKSAAFPILLVVILAFVAQRVISPDSGDKPPTYNEFLTMV
                     DKGEIDSVTVNTKNNSLDVTEKAADGSDSEGEKFETGYPPNTEQSLINQLREGDVSTT
                     VKGTGGSSLLSMLTYILPFLLFFGFWIFLMNQMQGGGSRVMSFGKSKAKKMAVDAPKI
                     TFKDVAGADEAVQELHEIKEFLQTPKKFQSLGARIPKGVLLYGPPGTGKTLLARAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKQNSPCIIFMDEIDAVGRHRGAGMG
                     GGHDEREQTLNQLLVEMDGFEIKDNIILIAATNRPDILDPALLRPGRFDRQVAVDRPD
                     RQGRKKILEVHSRGKPLAAGIDLDALAGQTPGFTGADLSNLINEAALLTARSGKREIT
                     QHELEEGIMRVIAGPEKKTRIMSEKERKITAYHEMGHAIVGHLLPNCDPVHKVSVISR
                     GQALGYTISLPAEDKFLTSRAELSETMAMTLGGRAAEELVFNEVTTGASNDLEKVTAT
                     AKQMVMRFGMSERLGPRVFGHDRGQPFLGRDMGSEPDYSDEIAREIDDEIRRIVENAH
                     QTARDILDERRDDLERISEILLVRETIDAEQFVALLEGASEEEVFPPESESAEEDYPS
                     EPEPVNTPPSGQTPDPQPSPPRPGYAGGMTDIRADQE"
     gene            complement(5007..6410)
                     /locus_tag="PLJ64_06185"
     CDS             complement(5007..6410)
                     /locus_tag="PLJ64_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin monoamine oxidase family protein"
                     /protein_id="HNC93103.1"
                     /translation="MSEARIETEVVVVGAGLAGLSAARELTRRGVEVAVVEARDRVGG
                     RILNESIGDGEIVELGGQWVGPGQDQVLALIQELGLETFPTWCEGRNVFERGGKARTY
                     SGTIPRLNPVSLAEVGMALKRLEKMAAKVDVTQPWAQTDLARWDAETFATWARRNAKT
                     PAARDLLRLAIQAVWAAEPEDVSLLHALFYVQSSGGSLEKMLDAEGGAQDSRVVGGTQ
                     LIAERMAAELGDAVHLGFPVRRVRWQRMPGDIEDAPVEFGPHPVTVEADELTVSARAA
                     VIALPPAVAGRLEYDPIMPALRDGLTQRMVQGTVVKCHAIYDEPFWRADGLSGQATSA
                     DGPVSVTFDNSPPSGTPGVLLAFLEGNAARQATDLPPAERREIVLDCLVRLFGPKAAN
                     PERFVDKAWAADEFARGCYGGYLPPGAWLMYGEGLRAPVGPLHWAGAETATVNAGYMD
                     GAISSGIRAALEITAGN"
     gene            6435..7247
                     /gene="folP"
                     /locus_tag="PLJ64_06190"
     CDS             6435..7247
                     /gene="folP"
                     /locus_tag="PLJ64_06190"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNC93104.1"
                     /translation="MKVMGIVNVTPDSFSDGGEFFDHEKAIEHGLELVRDGADVLDVG
                     GESTRPGAEEVSLEEELWRVIPVVEGLAGAAPVSIDTTKAEVAAAAIDAGAAMVNDVT
                     AFRGDMDMAAVCAGTGVEVVLMHMLGTPRTMQRDPRYRDVVAEVAEFLAVQVGLAETA
                     GVPRERIWIDPGIGFGKTVDHNLSLLRWTGAFARMDLKVLVGPSRKSFIGKLSNDASE
                     SRRLGGTIAACLAAMEGGAEMVRVHDVAEVAQAIRVASAIGSANGEPGGGPE"
     gene            7257..7643
                     /gene="folB"
                     /locus_tag="PLJ64_06195"
     CDS             7257..7643
                     /gene="folB"
                     /locus_tag="PLJ64_06195"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HNC93105.1"
                     /translation="MLQGNDGEVIIRLEGMIAHTNHGVTAAEQEVGQRMVFDLEMEPA
                     ECRATATDEVLDTVDYGEVTAWLIESAMDTRYRTLERLVTVLAEGLLENFRVDAVRLR
                     ATKPVPPVPVTMDGASVEIFRRRDEY"
     gene            7633..8163
                     /gene="folK"
                     /locus_tag="PLJ64_06200"
     CDS             7633..8163
                     /gene="folK"
                     /locus_tag="PLJ64_06200"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNC93106.1"
                     /translation="MSTDRPVYLGLGSNIGDRFGYLRDAIGRLEGKGLTVEAVSSAWE
                     TEPVGEILDQPDFLNAAIRVRSGLEPEELLDLLKQVEAELGRETGLPRHSPRVIDIDL
                     LLMGDLEFESDRLKLPHRETTSRRFVLAPLLELDPELALPDGTRLADAMATLESELEG
                     AEEGQGIRRAGPLLEE"
     gene            complement(8149..9246)
                     /locus_tag="PLJ64_06205"
     CDS             complement(8149..9246)
                     /locus_tag="PLJ64_06205"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNC93107.1"
                     /translation="MFVRWDGQRVTADGRPAKAGPAMALPGMEDAGLGPVRTFSAPEA
                     MGIRFHEVRAKSALNRVPEGPYGFGWTVNPYRGCSHACVYCFARPTHTYLGFNAGRDF
                     DREIVVKVNAPEVVRAELARPSWKRELVALGTNTDPYQWVESRYRLTRGVLEALDEAD
                     TPVSVLTKSPLVLSDLPIFERMNQARPTGVNLSVPTVDEKAWRATEPHTPSPQARLEA
                     VAELRRRGIPSGILIAPIMPGINDRPDQIEPILQAASDADADFVTPIALNLRKETRTV
                     FFDWLEAERPELLPEYGRLFDGRSRLPKIEQERVIAAARGRYRRRRVSERESPPMRPP
                     GVSGPPVSTNDEDPGSGTGRPGTARQASLFE"
     gene            9327..10418
                     /locus_tag="PLJ64_06210"
     CDS             9327..10418
                     /locus_tag="PLJ64_06210"
                     /inference="COORDINATES: protein motif:HMM:NF022470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2855 family protein"
                     /protein_id="HNC93108.1"
                     /translation="MSDSYDFAVRRDDMRETSVLETPGADREIDEGQILLEVDHFSLT
                     ANNVTYGAMGEAMNYWDFYPAEDDSLGRIPAWGYADVVVSKAEGIEEGARVFGYLPMS
                     THAVLEPGRIRESGFTDITGFRSGLPAVYNEYRLITGEKDDERERLVAIFMPLYGTSW
                     LLADWLNEVDFAGASQLVASSASSKTALGLGHALQVDFDNPIETVGLTSPGNLEFTTG
                     TGHWDAVVTYDDLDSIDADTPTIYVDFGGNAKLRHRIHSHFGDRLVASVAVGIADWES
                     MVPEEGDSLPGPQPQFFFAPDRVVKRNADWGQDGLGERLAKSQDRFTDRSREWMEVQT
                     GSGPDAIRESLLALMDGKVPPDRGISVNP"
     gene            complement(10470..11303)
                     /locus_tag="PLJ64_06215"
     CDS             complement(10470..11303)
                     /locus_tag="PLJ64_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HNC93109.1"
                     /translation="MNKWRYLLVSVLAALVAGLTIPAASSAWAPAASATVHPGVQTVT
                     EGAQCTSNFVFQNGSDVYLGQAAHCSGTGEATDTDGCQAGALPIGTAVEVTGASQPGT
                     LAYSSWNSMQAKGTTDASTCAFNDFALVRINPADVAGVNPSVPGYGGPTGLGAPGGLG
                     STVYSWGNSSLRQGIRQLSPKQGIVLSNDASGWSHNVYTLTPGIPGDSGSGFLNNSGQ
                     AFGTLSTLQLAPTVGANGVGDLAKELTWLKANEPAFAGIQLVNGTEPFKPNLVGAILG
                     G"
     gene            complement(11414..>11799)
                     /locus_tag="PLJ64_06220"
     CDS             complement(11414..>11799)
                     /locus_tag="PLJ64_06220"
                     /inference="COORDINATES: protein motif:HMM:NF015818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HNC93110.1"
                     /translation="HGALGSSDRDVIEQVADRADGLAGPVEPPARVHGDLWSGNLLWS
                     EGRPWLIDPAAHGGHRELDLAMLELFGSPGPAFYSAYEEVAPLADGYRERIGFWQLQP
                     LLVHAVLFGGGYGRSAAVTARACLG"
BASE COUNT         1991 a         3897 c         3946 g         1965 t
ORIGIN      
        1 atatcgaagc gttcaagcga atgggggagg tggccgaggc cggtctgccg gtcgaagccc
       61 tgctccagct gaccagggtg tacgcccagg ccgtacgacg gatcgccgac gccgaggtcc
      121 ggctcttcca tctcttcgtc catgagccga tgatccgtga cggcctgagc gagatcgaaa
      181 tggccgagga actcggcgac ctggcccgcc gcacctcgcc caccgcggtg ccgctcttcc
      241 agtacctgca tgaccggtat ctcaggtact tcctcgaaca ggacgtgatc gggcacatgg
      301 agtccgactt cggtggcgac accaacctcg accaggtcag gaccacgctc tgcttcatcg
      361 acctgaccgg gttcacccgg ttcaccgaac aggagggtga cgaaacggcg ttctcggtgg
      421 tcgagcagtt caccgaagcg gtcgaggcga ccctgccgcc cgaggccggg atcgtcaaga
      481 cgatcggcga cgaggtaatg gtggtctcgc ccgaccccgc ttcactggcc gaatgggcgg
      541 tcgggttcct ggccctgttc gaggaccggc cgaagccgcg ggccggcatc catcacggtg
      601 cggtcgtatt ccgggatggc gactactacg gcagccaggt caacctcgcc caccgggtcg
      661 tcaaccgggc ccttggcggc gaggtgctga tcaccgatgc cgtggcggcg gcgatcgagg
      721 atcacccgga cctcgagacc gatgcgatcg gcgaggtcga tctcaagggc ttcccggtcc
      781 cgaccccgct gtttctggtt cgtgcccgct cctgaccccg ggttcgaatc gttccggttc
      841 gggggcaagc ccgcacattt cattggcggc gcgatcaggg aatcgggact ggtatcagaa
      901 ggggatcgcg gtgtcgtgct cgtttcgggc ggggccgatt cggtcgcgct gctgctcggc
      961 ctccatctgg ttctcggcgc cgggcaactg gtcgcactcc acgtgaacta cggcctccgg
     1021 cccaccgccg acgatgacga gcggctggtc cgccggatct gtgagcagtt ggaggtcgaa
     1081 ctggttgtcc tgcgggcggg tgaggcagag ggaaacatgc aggcctgggc ccggaagatt
     1141 cggctggatg aagcggagcg gatccgcagc gagcggggtc tggactggat tgcggtcggc
     1201 cacaaccgct ccgatcaggt cgagaccttc atctaccgcc tcgtctcctc gccgggcgcg
     1261 aggtccctgc tggcgatgcc gccaaggtcg ggaaacctga tcaggccgct cctcacgctc
     1321 gatcgcggcc tgatccggac gatgcttgaa cgcgtcaccc attcggaaga cccgaccaac
     1381 gacgatccgg ccttcgcccg caaccggatc cggctggagg tcatgccggc gctcgagcag
     1441 gtcaactgcg gggcggagct caacgtagtg cggaccaggg ccgagctggc cgaggacgag
     1501 gaagccctga tgcggctggc ggtcgatgcc gtcgctttat ccggcgcggc cggtccggga
     1561 gggagccggg gaattcccgc gaagtcgctt gaggaccagc caccggccgt ccggcgccgc
     1621 atgattcgtc accttgccga agccgaactt gaccggccgg tcgccgtttc ggcggacctg
     1681 gcctctgaag tcctgcgttt ggccggaatg cccgaaggag gcaggctgga cctcggcggc
     1741 ggagactccc tgctggctga agcgggaagg gtacgggtct gcccgggcgg gggaacgggc
     1801 cacgacgcgg tgcccgagcc ggcccggatc gacctcaagg ccggtgttgc cgccttcggt
     1861 ggctgggaga tcgaatcctc aagggaagtc gaggccgagg tccgttccgc gttcggggat
     1921 ccctgggcgg catttctcga tctcgatgac ctgctcatct ggttgatcga gaagtcaccg
     1981 ggtcctgacg accttctgct tcaggtgcga ccgtggcgtt tcggcgaccg catcgagccg
     2041 cttggcatga gcggcagcaa gagcctccag gacgtcttca ccgacgcact cgtgcccgct
     2101 tccagaaggc agagctggcc ggtcctggtg gtcggcgaga aggtcatctg ggtgccgggg
     2161 ctggtccggt cacgccacct cctgatcgca gggccggacc gggatgtcat tagcctccac
     2221 gcccggccgc cgttctccat ctagacctct agactggcgg cgatggctgc gacgcctccg
     2281 aatgaactcg tctcggtcgg cgaggtgctg atcaccgctg aagacctcca gcggaaagtc
     2341 gccgacctcg gggccgaagt cagccgcgat tacgctggca aggacctggt catggtcggg
     2401 gtgctgcgcg gcgcggtggt cttcatcgcc gacctgctcc ggcacctcac cgtgccctgc
     2461 gagatcgact tcatggcggt ctccagctac ggctcgtcgc gtgattcttc aggcgtggtg
     2521 cggatcctca aggacctgga tgactcgatc accggccgtg acgtgctgat cgtcgaggac
     2581 atcgtcgatt cgggcctcac tctcaactac ctgatgcgga acctccaggc ccgcgacccg
     2641 gcgtcactgg aggtgtgcac cctcctgacc aagccggaac gccgccgggt cgacgttccg
     2701 atccgctaca ccggtttcga gatccccaat cagttcgtgg tcggttacgg gctcgatttc
     2761 gcccagcgct accggaatct cgatcatgtg gccttgctac cggaagacgc ggtccccgac
     2821 cgttagcggc ggcggcaaga aatcttcatc cgggaccgct ctcccatgcc ggttgccggg
     2881 ttctccctgc tagtctgaca attcggcctg aaggcaggta gagcctgtcg cgaggtcgtg
     2941 gaaagagacc caagatttga aacggttttt caagagcgcg gcattcccca tcctgctggt
     3001 ggtcatcctg gccttcgtcg cgcagcgcgt gatcagcccg gacagcggcg acaagccgcc
     3061 gacctacaac gagttcctga ccatggtcga caagggcgag atcgactcgg tcacggtcaa
     3121 caccaagaac aattcgctcg acgtgaccga aaaggccgcc gacggctccg actccgaggg
     3181 tgagaagttc gaaaccggct acccgccgaa caccgaacag agcttgatca accagctgcg
     3241 cgagggtgat gtctcgacca ccgtcaaggg caccggcggc agcagcctgc tctcgatgct
     3301 gacctacatc ctgccgttcc tgctcttctt cggcttctgg atcttcctga tgaaccagat
     3361 gcagggcggc ggctcgcggg tcatgagttt cggcaagtca aaggccaaga agatggccgt
     3421 ggacgccccg aagatcacct tcaaggacgt ggccggggcc gacgaggcgg ttcaggagct
     3481 ccacgaaatc aaggaattcc ttcaaacccc gaagaaattc cagtcgctcg gagcccggat
     3541 cccgaagggt gtgctcctct acggacctcc cggcaccggc aagaccctgc tcgcccgcgc
     3601 agtggctggc gaggccggcg ttcccttttt ctcgatttcc ggttcggact tcgtcgagat
     3661 gttcgtcggc gtgggcgcca gccgggtccg cgacctcttc gagcaggcca agcagaactc
     3721 gccctgcatc atcttcatgg acgagatcga cgccgtaggc cgccaccgcg gtgccggcat
     3781 gggcggcggt cacgacgaac gggagcagac cctcaaccag ctgctggtcg agatggacgg
     3841 cttcgagatc aaggacaaca tcatcctgat cgccgccacc aaccggcccg acattctcga
     3901 cccggccctg cttaggcccg gccgcttcga ccgccaggtc gcggttgacc ggcccgaccg
     3961 ccagggccgc aagaagatcc tcgaagtcca ttcccgcggc aagccgctgg ccgccggcat
     4021 cgacctcgat gccctggccg gccagacccc cgggttcacc ggtgccgacc tctccaacct
     4081 gatcaacgag gcggcgctcc tgaccgcccg ttcgggcaag cgcgaaatca cccagcacga
     4141 gctcgaagag ggcatcatgc gcgtgatcgc cggtccggag aagaagaccc ggatcatgtc
     4201 ggagaaggaa cggaagatca ccgcctacca cgaaatgggg cacgcgatcg tcggccactt
     4261 gctgccgaac tgcgacccgg tccacaaggt ctccgtgatc agccgcggcc aggcgctcgg
     4321 ctacacgatt tcgctgccgg ccgaggacaa gttcctgacc tcaagggccg agctgagcga
     4381 gaccatggcg atgaccctgg gtggccgcgc cgccgaggag ctggtcttca acgaggtgac
     4441 gaccggtgcc tcgaacgacc tcgaaaaggt caccgccacc gccaagcaga tggtgatgcg
     4501 cttcggcatg tccgagcgtc tcggcccccg tgtattcggc cacgaccgcg gtcagccctt
     4561 cctcggccgc gacatgggct cggaaccgga ttactcggac gagattgccc gcgagatcga
     4621 tgacgagatc cgccgcatcg tcgagaacgc gcatcagacc gcccgcgaca tccttgacga
     4681 gcgccgcgat gacctcgagc ggatctccga gatcctgctg gtccgcgaga cgatcgacgc
     4741 ggaacagttc gtcgccctgc tcgaaggtgc cagcgaggaa gaggtcttcc cgccggaaag
     4801 cgaatcggcc gaagaggact acccctcgga gccggagccg gtcaacaccc cgccctcggg
     4861 gcagaccccg gatccgcagc cttcaccgcc ccgccccggc tacgccggcg gcatgacgga
     4921 catccgcgcc gaccaggagt gacccaactg gcttgaagtt attcgctcca tgtgcggata
     4981 acttcaaggt agatctgttc cgcgcttcag tttcctgcgg ttatctcgag ggcggcccgg
     5041 attccggagc tgatcgctcc gtccatgtag ccggcattga ccgtcgcggt ctcggcaccg
     5101 gcccagtgga gcggaccgac cggggcgcgg agcccttcgc cgtacatcag ccaggccccg
     5161 ggaggcagat agccgccgta gcaaccgcgc gcgaactcgt cggcggccca cgccttgtcc
     5221 acgaaccgct ccgggttcgc ggcctttggt ccgaacagcc ggaccaggca atcgagcacg
     5281 atctcgcggc gttccgccgg aggtagatcg gtggcttggc gagccgcgtt tccttcgagg
     5341 aaggcgagca gaacaccggg ggtgccggac ggcggggagt tgtcgaaggt gaccgaaacc
     5401 gggccgtcgg cgctggtcgc ctggccggac agcccgtcgg cccgccagaa gggctcgtcg
     5461 tagatcgcat ggcacttgac caccgttccc tgaaccatgc gttgggtcag cccgtcccga
     5521 agggccggca tgatcgggtc gtactcgagc cgtccggcga ccgcgggcgg gagcgcgatc
     5581 accgccgccc gggcggaaac ggtcagctcg tcggcctcga ccgttaccgg atgaggcccg
     5641 aactcgaccg gggcatcctc gatgtcaccc ggcatccgct gccagcgaac ccggcggacc
     5701 ggaaacccga gatggacggc gtcaccgagc tcggccgcca tcctttcggc aatcagctgg
     5761 gtccccccga ccacccgtga gtcctgggcg cccccctcgg cgtccagcat cttttcgagt
     5821 gacccgccgg aggactggac gtagaagagg gcgtggagca gcgagacatc ttccggctcc
     5881 gccgcccaga ccgcctgaat cgcgagtcga agcaggtccc tggctgccgg ggtcttcgca
     5941 ttgcgccgcg cccaggtggc gaaggtctcg gcatcccagc gggccagatc ggtctgggcc
     6001 cagggctgcg tgacgtccac cttcgcggcc atcttttcga gccgcttcag ggccatcccg
     6061 acctcggcca gggaaaccgg attcagcctg gggatcgtgc ccgagtaggt gcgagccttg
     6121 ccgccccgct cgaacacgtt tctgccttcg caccaggtcg ggaaagtctc aagcccgagt
     6181 tcctgaatca acgcgagcac ctggtcctgg cccggcccga cccattggcc acccagctcg
     6241 acgatttcac cgtcgccgat cgattcgttc aggatccggc ccccgacccg gtcccgggct
     6301 tcgaccacgg ccacctcgac gccccgccgc gtcagttcac gggccgccga aaggccggcc
     6361 agcccggcgc ccaccaccac aacctcggtc tcgatcctcg cctcgctcat gcccggcagt
     6421 ctagagtcgc gtaaatgaag gtcatgggca tcgtcaacgt gactccggac tcgttctcgg
     6481 acggaggcga attcttcgat cacgaaaagg cgatcgagca tggcctcgag ctggtcaggg
     6541 acggggcaga cgtgctcgac gtcgggggcg agtccacccg gcccggggcg gaagaggttt
     6601 cactcgagga agagctttgg cgggtgattc ccgtggtcga ggggctggcc ggggcggcgc
     6661 ccgtctcgat cgacacgacc aaggccgagg ttgccgcggc ggcaatcgat gccggggcgg
     6721 ccatggtcaa tgacgtaacg gcattccggg gggacatgga catggccgcg gtctgcgccg
     6781 ggaccggggt ggaggtggtc ctgatgcaca tgctcggaac cccgaggacg atgcagcgcg
     6841 accccaggta ccgggacgtc gtcgccgaag tcgcggagtt cctcgcggtc caggtcggtc
     6901 tggccgagac ggcgggggtt ccacgcgaga ggatctggat cgatcccggc atcggattcg
     6961 gcaagacggt tgatcacaac ctgtccctgc tcaggtggac cggggcgttc gcccgcatgg
     7021 acctgaaggt gctggtcggt ccttcgcgca agtcgttcat cggcaagctc tcgaacgatg
     7081 cgtcggaatc ccgtcggctg ggcggcacga tcgccgcctg cctggccgcg atggagggcg
     7141 gggcagagat ggtccgggtt cacgatgtcg ccgaggtcgc ccaggcgatc cgggtggctt
     7201 cggccatcgg ttccgcgaac ggtgaaccgg gcggcggccc ggagtagatt cccctcatgc
     7261 ttcagggaaa tgacggcgaa gtgatcatcc gcctcgaggg catgatcgcc cacaccaacc
     7321 acggggtgac cgcagccgaa caggaggtcg gccagcgcat ggtcttcgac ctcgagatgg
     7381 aaccggccga atgccgggcc accgccaccg acgaggtact ggacacggtc gactacggcg
     7441 aggtgaccgc ctggctgatc gagtcggcga tggacacccg ctaccgcacc ctcgagaggc
     7501 tggtgacggt gctggccgag gggttgctcg agaatttccg ggttgacgcc gtccggcttc
     7561 gggcgaccaa gcccgtgccg ccggtgccgg tgaccatgga cggcgcctcg gtcgagatct
     7621 tccgtcgtcg cgatgagtac tgatcgtccg gtttatctcg gactcggttc caacatcggc
     7681 gaccggttcg gttacctgcg cgacgcgatc ggccggcttg aaggcaaggg cctgaccgtc
     7741 gaggccgttt cctccgcctg ggaaacggaa ccggtcggcg agatcctcga ccagccggat
     7801 ttcctcaacg ccgcaatccg ggtccgcagc ggtctcgagc cggaagagct gcttgacctg
     7861 ctcaagcagg tcgaagcgga actcgggcgc gagaccggcc tgccaaggca ttcaccacgg
     7921 gtgatcgaca tcgacctgct gctcatgggt gacctcgaat tcgagtccga tcggctgaag
     7981 cttccgcacc gggaaaccac ctcccgccgc ttcgtcctcg ccccgctgct cgagctcgac
     8041 ccggaactcg ccctgccgga cggaacccgc ctggccgacg ccatggccac ccttgaatcg
     8101 gagctcgaag gcgccgaaga gggtcagggg atccgacggg ccggacccct actcgaagag
     8161 tgaggcctgc cgggcggttc cgggccggcc ggttcctgag ccggggtctt cgtcattggt
     8221 cgacaccggt ggtcccgata cacccggcgg gcgcatcggc gggctctccc tttcgctgac
     8281 ccgccgccgg cggtaacggc cccgggcggc cgcgatgacg cgctcctgct cgatcttcgg
     8341 cagccgggag cggccgtcaa agaggcgccc gtattcgggg agcagctccg ggcgctcggc
     8401 ctccagccag tcgaagaaca cggtccgcgt ctccttgcgg aggttgagtg cgatcggggt
     8461 gacaaagtcg gcgtcggcat cgctggccgc ctggagaatc ggttcgatct gatccggacg
     8521 gtcgttgatt ccgggcatga tcggggcgat caggatgccc gaggggatgc cccgccgcct
     8581 caactcggcc acggcttcca gccgggcctg cgggctcggg gtgtgtggtt cggtcgcccg
     8641 ccaggccttt tcgtcaacgg tcggcaccga gaggttcact ccggtcggcc gggcctggtt
     8701 catccgttcg aagatcggca ggtcggagag caccagtggt gacttggtca ggaccgagac
     8761 cggggtgtcg gcctcatcga gcgcttcgag cacgccgcgg gtcagccggt acctgctttc
     8821 gacccattgg taggggtcgg tgttggtgcc gagcgcgacc agctcgcgct tccatgaagg
     8881 tcgcgcgagt tcggcccgga ccacctccgg ggcattgacc ttgaccacga tctcccgatc
     8941 gaagtcccgg cccgcgttga agccgaggta agtgtgggtg gggcgggcga agcagtagac
     9001 gcaggcgtgg ctgcagccgc ggtacgggtt gactgtccag ccgaagccgt aagggccttc
     9061 cggcacccgg ttgagggccg acttggcccg gacttcgtgg aacctgatcc ccatcgcttc
     9121 cggagccgag aaggtgcgta ccggaccgag cccggcgtct tccatccccg gcaaggccat
     9181 tgccgggccg gccttcgccg ggcgcccgtc ggcggttacc cgttgcccgt cccatcgcac
     9241 gaacatatgt tcgcatatcc ggcgacccgg ttcgctgatg caacaccctc tgcaagtgtt
     9301 tcagtcaaat cgatagtctc accgacatgt ccgactctta tgactttgca gtccgccgcg
     9361 acgacatgcg ggaaacctcg gttctcgaga ctccgggagc cgaccgggag atcgacgaag
     9421 gccagatcct gctcgaggtc gaccacttct cgctgaccgc caacaacgtc acctacggtg
     9481 cgatgggcga ggcgatgaac tactgggatt tctatccggc cgaggatgat tcactgggcc
     9541 ggatcccggc ctggggatat gccgacgtgg tggtctcgaa ggccgaggga atcgaggaag
     9601 gtgcccgggt cttcgggtac ctgccgatgt cgactcacgc cgtcctcgag ccgggccgga
     9661 tccgggagtc gggtttcacc gatatcaccg gtttccgctc cgggctcccg gcggtctaca
     9721 acgaataccg cctgatcacc ggggagaagg acgatgaacg ggagcggctg gttgcgatct
     9781 tcatgcctct ctacggaacc tcgtggcttc tggccgactg gctgaatgaa gtcgatttcg
     9841 ccggcgcctc ccagctggtt gcctccagcg cttcaagcaa gacagcgctc ggtctcggcc
     9901 acgcgctgca ggtcgatttc gacaatccga tcgagacggt gggcctgacc tcgcccggca
     9961 atctcgagtt caccacgggc accgggcact gggatgcggt cgtcacctat gacgacctcg
    10021 acagcatcga cgcggacacg ccgaccatct acgtcgattt cggcggcaac gcgaagctca
    10081 ggcaccggat tcacagtcac ttcggtgacc ggctcgtggc cagcgttgcg gtcgggatcg
    10141 ctgactggga atcgatggtt ccggaagaag gcgattctct gcccgggccc cagccgcagt
    10201 tcttcttcgc gcccgaccgg gtcgtcaagc gcaacgccga ctgggggcag gacggcctcg
    10261 gcgagcgcct ggccaaaagc caggaccgtt tcaccgaccg cagccgcgag tggatggaag
    10321 ttcagacggg aagcggaccg gatgcgatcc gggagtccct gctcgccctg atggacggca
    10381 aggtaccccc ggatcgaggc atctcggtca atccctagaa cccgtggcca agacgtcccg
    10441 aggacggagt cttggccacg ggttccagct cagccgccga ggatcgcgcc gaccaggttc
    10501 ggcttgaagg gctcggttcc gttgaccagc tgaatgccgg cgaaggccgg ctcgtttgcc
    10561 ttgagccagg tcagttcctt cgcgaggtca ccgactccgt tcgcgcccac cgtgggtgcg
    10621 agctgcaggg tgctgagcgt gccgaaggcc tgaccgctgt tgttcaggaa gccgctgccg
    10681 gagtcaccgg ggatgccggg ggtcagggtg tagacgttgt ggctccagcc gctggcgtca
    10741 ttgctgagaa cgatgccctg cttcgggctg agctggcgga tgccctggcg gagcgacgag
    10801 ttgccccagg agtagacggt cgagccaagt ccgcccggcg caccaagccc ggtcggtccg
    10861 ccgtagccgg ggaccgaggg gttgacgccg gcgacgtcgg ccggattgat cctgaccagc
    10921 gcgaagtcat tgaaggcgca ggtcgaggcg tcggtcgtgc ctttggcctg catcgaattc
    10981 cacgagctgt aggcgagggt gcccggctgg ctggccccgg tcacctcgac cgcggtgccg
    11041 atcggcaggg cgccggcctg acagccatcg gtgtcggtcg cctcgccggt gccggaacag
    11101 tgagcggcct ggccgaggta gacgtcggac ccgttctgga agacgaagtt cgaggtgcac
    11161 tgggcgccct cggtcacggt ctggacgccg gggtggacgg tcgccgaagc agccggtgcc
    11221 caggccgacg aggccgccgg gatggtcagg ccggccacaa gcgccgcaag gacagaaaca
    11281 agcaggtatc tccatttgtt catgcgagat ctactacccc atcggggtag ccataaacac
    11341 ttcgtttctc ataaagggtc cgtgagatgc ggttggtcgg cgaatatcgc cgaccaactg
    11401 cacttcgatt ccctcagccg agacaggccc gggcggtgac cgcagcgctt cggccgtaac
    11461 caccaccgaa gaggaccgca tgaaccagca gcggctggag ctgccagaag ccgatccgtt
    11521 cccggtatcc gtcggccagg ggcgcgactt cctcgtaggc cgaatagaaa gcgggtccgg
    11581 ggctgccgaa aagctcgagc atcgccaggt cgagttcgcg gtggccgccg tgagcggccg
    11641 ggtcgatcag ccagggccgg ccctcgctcc agagcaggtt gccggaccag aggtcgccgt
    11701 ggacccgggc cgggggttcg accggcccgg ccagaccgtc ggcccggtcg gcgacctgct
    11761 cgatcacatc gcgatccgag gacccgagcg cgccgtgct
//
LOCUS       DAOQKN010000068        11753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_2555_length_11753_cov_5.951701, whole genome shotgun sequence.
ACCESSION   DAOQKN010000068 DAOQKN010000000
VERSION     DAOQKN010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 11753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11753
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2555_length_11753_cov_5.951701"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1444)
                     /locus_tag="PLJ64_06225"
     CDS             complement(<1..1444)
                     /locus_tag="PLJ64_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-aminobutyraldehyde dehydrogenase"
                     /protein_id="HNC93111.1"
                     /translation="MAITAKLRLQNMIDGEFVDPVDGGSEEVINPSTGEVIAESPLSG
                     VEDVNRAVAAAKRAFENGWATTTPRERSDRLLALADAIIENGDELTDLESADAGKPRQ
                     AFIDEELDTTADHIRYFATAARNLEGKAAMEYLEGHTSFIRREPVGPVAQITPWNYPL
                     MMAAWKLGPALATGNTLVLKPAESTPVSTLHTLARICAEVFPPGVTNFIGGHGDPAGS
                     TLVTHPDIKMVSLTGSIGTGKWIAKAAADSLKRVHLELGGKAPVIVFDDADLDAVYET
                     IAGTALFNAGQDCTAATRILAGPKVYDDVVNGLAGEAKGYVMGDLDSAETTLGPVNSA
                     RQLEHVTGFFDRAPSHAQIVTGGKQAEGLPGFFVEPTVVADLRQDDEMVQNEIFGPAM
                     TVQRFTDEEEAIRWANGTKYGLASSVWTRDVGRAMRVTNALRFGAVWVNDHILMAVEM
                     PHGGYNESGYGKDMSMYSLEDYTQIKHVMIN"
     gene            complement(1449..2570)
                     /gene="ald"
                     /locus_tag="PLJ64_06230"
     CDS             complement(1449..2570)
                     /gene="ald"
                     /locus_tag="PLJ64_06230"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HNC93112.1"
                     /translation="MKVGVIKEIKQDEYRVAITPAGVREFVEHGHEVIIEDGAGEGSA
                     IPNTSYEAQGARILPDASSVFEAAEMILHVKELQASEIEMLKPGHLLFTYLHLAPDPE
                     QTAGLMKSGSTCVAYETVEDAEGRLPLLAPMSEIAGRIATQAGAFMLEKPLGGRGVLL
                     GGVPGVAAANVVVIGGGVVGTNAAFIAMGMAADVFILDRSLDRLRELDLHYAREASTV
                     YSTTLAVEELLPKADLVIGAVLVHGARAPWVIKREQLKLMKPGAVLVDVAIDQGGCFE
                     TSRPTTHRDPTYEVDGIIHYCVANMPGAVPITSTQALTNATLPYALALADNGIQAAVQ
                     RDPGLRPGVNVAAGKVTHEGVAEGTGQQYVPVEEALGWV"
     gene            complement(2642..4024)
                     /locus_tag="PLJ64_06235"
     CDS             complement(2642..4024)
                     /locus_tag="PLJ64_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNC93113.1"
                     /translation="MATTTPESTSIQALQEQARRHLWMHFSRMGGYDDQHEIPIIARG
                     DGAYVYDAHGKRYLDGLSGLFCVNAGHGRTELAEAAARQYEELDFFVIWSYAHPRAIE
                     LATKIASMTPGDLNRVFFTSGGGEAVESALKLARNYHRMRGNGQKAKHIAREVAYHGT
                     TLGALSATGITNLRSQFEPLAPGGCHVPNTNIYRWPEDREPIWAADAIEERILFEGPE
                     TVSAVILEPVQNAGGCFVPPDGYFQRVREICDKYDVLLISDEVICAWGRLGHYFGCER
                     FGYQPDIITSAKALTSSYVPMGAMIASDKVAEPFMEGTASFAHGFTFAGHPVGAAVAL
                     ANIEIFEREDLCGHVLAKEGEFRGMLESLRDIPIVGDVRGAGYFQAIELVKDQETKQS
                     FNDEESEKLLRGFLSGALYERGLICRADDRGDPVIQFSPPLICDTEQFEEIHDVLRPV
                     LTEAASMMRN"
     gene            4184..5737
                     /locus_tag="PLJ64_06240"
     CDS             4184..5737
                     /locus_tag="PLJ64_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HNC93114.1"
                     /translation="MESPEVSSRPEPAVPNQGDQPHLKADAISFADGLAIALASTAPA
                     YSLAAVIGSIVVLVGLQAPAALLVAFVPMFFIAAAFYYMNKADQDCGTCFAWVTRAIG
                     PQAGWMTGWAICVTGILVVGSLADVAATSFFDLIGADSLMNSTAAVTGTAVALMIVMT
                     GVCVWGTEISANLQKVLMVVQIVPLILFIVVALFKVWSGDAPAGSVDPSLSWLNPFEI
                     PTGALSGAMLLAVFIYWGWESAVNLNEETEGDVDKPGLAGVISTTLLLITYVGVAFAI
                     VAWGGVSGAGKFDDNAAILATYAKQVLGGTLGLLVLLSVVVSALASTQTTILPSSRTS
                     LSMARAEAMPHALSEISGRFFTPVVSTVTIGVLAILWYLPSKLISENFLFDSLSALSL
                     MIAFYYAMTGYACAFYYRRELFKSVKNLLFVGLAPVIGALLLTYLFIKSLVDWSDPAN
                     SYSGASWFGFAPPAVIGVGFLLLGAMLLLAWRIHQEEPFFKRRREVADPGLLEGKAGA
                     AVALAGPEN"
     gene            complement(5734..7215)
                     /locus_tag="PLJ64_06245"
     CDS             complement(5734..7215)
                     /locus_tag="PLJ64_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93115.1"
                     /translation="MKTSADHKLPRLARCLALLAMLVALACFGGRADAAVTGDNPCPT
                     SASNSLSWMGASVGQDFTIPSLRHQADSSQVPATYPMTVLRPADTVTYPGPRPVVVFQ
                     HGLGGNRCALWWAAQYLAGHGFVTTVHRSLPGNEQTASDAYRSGVDAVRSVIAYLKGL
                     QVSDPNLGPMIDLGRLGLSGHSMGSIISSQLQGDPSLGIKAIVAFDTLRRYVQGDPGA
                     ARIDCQSDPVPATQVTPSVPALSFAMDEPCAYKPDFKPADLKLSGPRWWSEHGVPNMQ
                     LVMKGYPHGGFTDGASADKKLDLAHWVLPWFERWLNGDKSQEQLLAAPIVNGRPVVDL
                     LSSEFLSSACLDGIADSEDLRSWFDAGSPATSGAGRCDSPPGQSPDPAHPAPALTNRK
                     LVKLVRLSSSRGWVRAGGKARLKLRITVKGMGQPVKNLNLLVSSSNRRVRVAGTRKVQ
                     RINPASTRVVVLTVRAGRKARNKVRVKVTVAGRTVGRTLRIRR"
     gene            7548..8672
                     /locus_tag="PLJ64_06250"
     CDS             7548..8672
                     /locus_tag="PLJ64_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC93116.1"
                     /translation="MEGVTKKFGDFTAVSEMDLDIADGEFFTMLGPSGCGKTTTLRMV
                     AGFEQPTTGKVLIDGTDVAGQPAYKRPTNTVFQSYALFPHMTLEENVGFGLKRKKVPK
                     AEIKQRVANELERVGLSREAKRKPNQLSGGQQQRVALARALVNLPKVLLLDEPLGALD
                     LKLRKGLQVQLKQIQRDVGITFVYVTHDQEEALTMSDRIAVMNEGRIEQCADPETVYD
                     RPATTFVAGFIGVSNLMPAEVKGSNEIQVVNGPLVPTPDAAKYSGGEKCFAVVRPEKL
                     TVSLPENAPSDQPGVDGIVESSLYLGTSTQMAVRVTEDVMMTVLVPNASEDARQHLPG
                     GGAKVRLNWLPEHMHLVAESESATAATDPTPTEKVTEGVN"
     gene            8672..9826
                     /locus_tag="PLJ64_06255"
     CDS             8672..9826
                     /locus_tag="PLJ64_06255"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HNC93117.1"
                     /translation="MSESNVKRVRLLALAVLALAVSMVLVACGGGGIDEGSDNSDVTT
                     ATGGDPQGSLKISNWPLYIDGATIKDFQNDTGINTTYTEDVNDNNEFFGKVQQQLAQG
                     DSGGRDIIVVTDWMAEKMYNLGYIQNLDKAKLPTVEKNLIPSLQSPSFDPERKYSVPW
                     QSGMTGLVVRKDLAPDVKSINDLFDPKYKGKVTLLTEMRDTVPLVMAADGVDPKEATT
                     QDWLDAIDKIKENVDNGQIRRFTGNDFVQDLAKGDVVAAIGWSGDAVQAQSDNENIEY
                     VQPEQGCSIWSDNMLIPVGAPNPAAAYKFMNYVYEPENQAQITKYVNYVSPVKGVREI
                     LEKQDPAIANNNLIFPSEQFTKNCFAQVSPPGDEAAQQEVEQAFQDVVTG"
     gene            9893..10759
                     /locus_tag="PLJ64_06260"
     CDS             9893..10759
                     /locus_tag="PLJ64_06260"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC93118.1"
                     /translation="MTIRRRLIPYWLLAPGLIWLVLFFVIPMYYMGELSLKTGSLETG
                     YQLTWAFENYTDSISEYSTQFIRAFWYAGVATFFCLLIGYPLAYAIAFKAGRWKNLML
                     FAVVAPFFTTYLIRTIAWKTILDDSSPVVSFLKTIHLVPQDGRVLATSAAVIAGLTYN
                     FLPFMVLPLFTALERLDQRLLEAGKDLYASATQTFWRITLPLTMPGIIAGILLTFIPA
                     AGDYVNATFLGGTNQAMIGNVIQSKYLVVNDYPDAAALSFTLMAIILVALLFYIKFAG
                     SEALMGEEEETR"
     gene            10756..11604
                     /locus_tag="PLJ64_06265"
     CDS             10756..11604
                     /locus_tag="PLJ64_06265"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC93119.1"
                     /translation="MKAWRWTKDHMVLFVGLLALAYMLIPILVIAVFSFNNPEGRYNF
                     EWVGFTTEYWGKPFAITELTDALLVSIRLAFFTAIGATILGTLIAIALVRYQFFGRRA
                     ANLLIVIPMATPEVVIGASLLSMFLIYGAKLGFGTLLIAHIMFSISFVVIVVRSRLIG
                     FDRRLEEAAADLGANAFTTFRKVTLPLIAPGVISAALLSFALSIDDFVISNFNSGSTV
                     TFPLYIFGANQRGIPVQVNVIATMLFAVTVIAMIAVILQQRRAEKAAGLRPDEIADKK
                     AISLPV"
BASE COUNT         2017 a         3802 c         3793 g         2141 t
ORIGIN      
        1 tattgatcat cacgtgcttg atctgggtgt agtcctcgag ggagtacatg ctcatgtcct
       61 tgccgtagcc ggattcgttg tatcccccgt ggggcatctc gaccgccatc aggatgtggt
      121 cgttcaccca gacggcaccg aagcggagcg cgttggtgac ccgcattgcg cgaccgacat
      181 cacgggtcca gaccgaggag gccaggccgt acttggtgcc gttggcccac ctgatcgctt
      241 cttcctcgtc ggtgaagcgc tgcacggtca tcgccggccc gaagatctcg ttctggacca
      301 tctcgtcgtc ctgccggagg tcggcgacca cggtcggctc gacgaagaac ccgggcagac
      361 cttcggcctg cttgccaccg gtgacgatct gggcatgcga gggcgcgcgg tcgaagaagc
      421 cggtcacgtg ttccagctgc cgggccgagt tgaccgggcc gagcgtggtc tcggcggaat
      481 cgagatcgcc catcacgtag cccttcgcct cgccggcgag gccgttgacc acgtcgtcgt
      541 agaccttggg ccccgccagg atccgggttg ccgcggtgca gtcctgaccg gcgttgaaga
      601 gcgcggtgcc ggcgatcgtc tcgtagaccg cgtcgaggtc ggcgtcgtcg aagacgatca
      661 ccggggcctt gccgccgagt tcgaggtgaa cccgtttgag cgagtcggcc gcggccttcg
      721 cgatccactt gccggtgccg atcgatccgg tcagcgagac catcttgatg tcgggatggg
      781 tgaccagggt tgaaccggcc ggatcaccgt ggccgccgat gaagttggtc acaccgggcg
      841 ggaagacctc ggcgcagatc cgggccagcg tatgcagggt cgagaccggt gtcgactcgg
      901 ccggcttcag gaccagtgtg ttgccggtcg cgagcgccgg accgagcttc caggcggcca
      961 tcatcagtgg gtagttccag ggggtgatct gggcgaccgg accgaccggt tcgcggcgga
     1021 tgaacgaggt gtgaccttcc aggtactcca tcgcggcctt gccttcgagg ttgcgggcgg
     1081 cggtcgcgaa atagcgaatg tggtcggcgg tggtgtcgag ctcctcgtcg atgaaggcct
     1141 ggcgcggctt gccggcatcg gccgattcca gatcggtcag ctcgtcgccg ttctcgatga
     1201 tcgcatcggc aagggcaagc aggcggtcgg agcgctcccg cggggtggtg gtcgcccagc
     1261 cgttctcgaa ggcgcgtttg gcggccgcca ccgcccggtt tacatcctcg accccggaga
     1321 ggggtgactc ggcgatcact tcaccggtcg aggggttgat cacctcctcc gagccgccgt
     1381 caaccgggtc gacgaactcg ccgtcgatca tgttctgaag ccggagcttc gcggttatcg
     1441 ccatgggatc agacccagcc gagtgcttcc tcgaccggta cgtactgctg gccggtgcct
     1501 tcggcgacgc cttcgtgggt gaccttgccg gccgccacgt tgaccccggg ccggaggccc
     1561 ggatcccgct ggaccgccgc ctggatgccg ttgtcggcga gggccagggc gtagggcagg
     1621 gtcgcgttgg tgagggcctg ggtcgaggtg atcggcaccg cgcccggcat gttcgccacg
     1681 cagtagtgga tgatgccgtc gacctcgtag gtcgggtcac ggtgggtggt cggccgggaa
     1741 gtctcgaagc agccaccctg gtcgatcgca acgtcaacca gaaccgcgcc cggcttcatc
     1801 agcttcagct gttcgcgctt gatcacccag ggagcgcggg cgccgtggac cagaacagcg
     1861 ccgatcacga ggtcggcctt gggcaggagc tcttcaaccg cgagggtggt cgagtaaacg
     1921 gtcgaggctt cgcgggcgta gtgcaggtca agttcacgca ggcggtcgag cgaacgatcg
     1981 aggatgaaga cgtccgccgc catgcccatc gcgatgaaag cggcattggt gccgaccacg
     2041 ccgccaccga tgaccacgac gttggccgcc gcgactccgg gcacgccgcc gagcagcacg
     2101 ccccggcccc ccagcggctt ctccagcatg aaggcgccgg cctgggttgc gatccggccg
     2161 gcgatctcgc tcatcggcgc cagcagcggc agccggccct cggcgtcttc gaccgtctca
     2221 tacgcgacgc aggtcgagcc ggacttcatc aggccggcgg tctgctcggg gtcgggggcg
     2281 aggtgcaggt aggtgaagag cagatgcccg ggcttcagca tctcgatctc ggatgcctgc
     2341 agttccttca catgcaggat catctcggcc gcctcgaaga cggaactggc gtcgggcagg
     2401 atccgcgcac cctgcgcctc gtatgaggta ttcgggatgg cgctgccttc gccggcgccg
     2461 tcctcgatga ttacctcgtg gccgtgctcg acgaattcac gcaccccggc cggcgtgatc
     2521 gccacccggt actcgtcctg cttgatctcc ttgatgacgc cgaccttcat ctcttacctc
     2581 gtcctctctg gtggtccgct ggggtgactt ttccgccggg cggggtgaat gggtgaatcg
     2641 atcagttcct catcatggac gcggcctcgg tgaggaccgg cctgaggaca tcatgaattt
     2701 cctcgaactg ctcggtgtcg cagatgagcg gcggcgagaa ctggatcacg gggtctcccc
     2761 ggtcgtcggc ccggcagatc aggccgcgtt cgtagagcgc ccctgaaagg aagccgcgca
     2821 acagcttctc tgactcttcg tcgttgaagc tctgcttcgt ttcctggtcc ttgaccagct
     2881 cgatcgcctg gaagtaaccg gcgccgcgca cgtcaccgac gatcgggatg tcccggagcg
     2941 attccagcat gccgcggaat tcgccctcct ttgccagcac gtggccgcag aggtcctcgc
     3001 gttcgaagat ctcgatgttg gccagggcga ccgccgcgcc gaccgggtgg ccggcgaagg
     3061 tgaagccgtg cgcgaaggag gcggtgccct ccatgaacgg ctcggcgacc ttgtcggagg
     3121 cgatcatcgc gcccatcggc acgtaggagg aggtcagggc cttggccgag gtgatgatgt
     3181 ccggctggta accgaaacgc tcgcagccga agtagtggcc gagccggccc caggcgcaga
     3241 tcacttcgtc ggagatcagc agcacgtcgt acttgtcgca gatttcgcgg acccgctgga
     3301 agtaaccgtc gggcggcacg aagcagccgc cggcgttctg gaccggttcg aggatcaccg
     3361 cggagacggt ctcggggccc tcgaagagga tccgctcctc gatcgcatcg gctgcccaga
     3421 tcggctcccg atcctcgggc cagcggtaga tgttcgtgtt cggcacgtgg catccgcccg
     3481 gagccagcgg ctcgaactgg gaacgaaggt tggtgatgcc ggtcgccgaa agggcgccga
     3541 gagtggtgcc gtggtaggcc acctcgcggg cgatgtgctt ggccttctgg ccgttgcctc
     3601 gcatgcggtg gtagttgcgg gccagcttga gagcggactc gaccgcctcg ccgccgccgc
     3661 tggtgaagaa gacccggttg aggtcgcccg gggtcatcga ggcgatcttc gtggcgagct
     3721 cgatcgcccg gggatgggcg tagctccaga tcacgaagaa gtcgagttcc tcgtactggc
     3781 gggcagccgc ttcggccagt tcggtgcggc cgtggccggc gttcacgcag aacagcccgg
     3841 aaagcccgtc gaggtaccgc ttgccgtggg cgtcgtagac ataggcgccg tcgcccctgg
     3901 cgatgatcgg aatctcatgc tggtcgtcgt aaccgcccat gcgggagaag tgcatccaga
     3961 ggtggcggcg agcctgttcc tgcagagcct ggattgaagt tgattcggga gttgtggtgg
     4021 ccacgcgctc ctccttttcc ggatggtgaa gttgggggtg tggacaaacc ctatgtcggg
     4081 taagtcacac aggcaagtcg acggaacgta aaaagggagc gccgctccgt taccggacgt
     4141 cggagtttcg gcctggcgcc gggtttgcca ctaggtttcc cgcatggaga gtccagaggt
     4201 ttcttctcgt ccggagcctg ctgtccccaa ccagggcgac cagcctcacc tcaaggccga
     4261 cgcgatcagt tttgccgacg gtctcgcaat cgccctcgcc tcgaccgccc cggcctactc
     4321 cctggccgcg gtcatcggct cgatcgtcgt gctggtcggg ctgcaggccc cggccgccct
     4381 gctggtcgcc ttcgtgccga tgttcttcat cgctgccgcc ttctattaca tgaacaaggc
     4441 cgaccaggac tgcggaacct gcttcgcctg ggtgacccgg gcgatcggac cccaggcggg
     4501 ctggatgacc ggctgggcga tctgcgttac cggcatcctc gtggtcggct cgctggccga
     4561 cgtggcggca accagcttct tcgacctgat cggggccgac agcctgatga acagcaccgc
     4621 ggcagtgacc ggcaccgccg tcgccctgat gatcgtgatg accggggtat gcgtctgggg
     4681 gaccgagatt tcggccaacc tccagaaggt tctgatggtc gtgcagatcg tgccgctgat
     4741 cctgttcatc gtcgtcgccc tgttcaaggt ctggagcggc gatgccccgg ccggttcggt
     4801 cgacccgtcg ctttcctggc tgaacccctt cgagatcccg accggggcgc tttccggtgc
     4861 gatgctgctc gcggtgttca tctactgggg ctgggaaagc gcggtgaacc tgaacgagga
     4921 aaccgagggg gacgtcgaca agcccggact ggccggcgtg atctcgacca cgctgctgct
     4981 gatcacctac gtcggggtcg ctttcgcgat cgtcgcctgg ggcggcgtgt ccggggccgg
     5041 gaagttcgat gacaacgccg cgatcctcgc cacctacgcc aagcaggtcc tgggcggaac
     5101 cctcgggctg ctggtcctgc tttcggtggt ggtctcggca ctcgcctcga cccagaccac
     5161 gatcctgccc tcctcccgca cctcgctttc gatggcccgg gcggaagcga tgccccatgc
     5221 gctcagcgag atctcgggcc gcttcttcac cccggtcgtt tccaccgtga cgatcggcgt
     5281 cctggcgatc ctctggtacc tgccctcgaa gctgatcagc gagaacttcc tcttcgattc
     5341 cctttcggcc ctctcgctga tgatcgcctt ctactacgcg atgaccggtt acgcctgtgc
     5401 cttctactac cgccgggagc tcttcaagtc ggtgaagaac ctgctcttcg tcggcctcgc
     5461 cccggtgatc ggagccctgc tgctcaccta cctcttcatc aagtcgctgg tcgactggtc
     5521 cgatccggcc aattcctaca gcggcgcctc ctggttcggc ttcgccccgc cggcggtgat
     5581 cggggtcggc ttcctgctgc tcggcgcgat gctcctgctg gcctggcgga tccatcagga
     5641 agagccgttc ttcaagcggc gccgggaggt cgccgacccg gggctgctgg agggcaaggc
     5701 cggagcggcg gtcgccctgg ccggaccgga aaactagcgc cggatccgca gcgtcctgcc
     5761 gacggttctg ccggccacgg tcaccttgac ccggaccttg ttccgggctt tccttccagc
     5821 cctcacggtc agcacgacca cacgggtgct ggccggattg atccgctgaa ccttcctcgt
     5881 gccggcgacg cgcacgcgcc ggttcgagga gctgaccagc aggttcaggt tcttcaccgg
     5941 ctggcccatg cccttgacgg tgatccgcag tttcaatctg gccttgccgc cggcccggac
     6001 ccagccgcgg gatgaactca gcctgaccag cttgaccagc ttgcgattgg tgagggcggg
     6061 agcgggatgg gcgggatccg gtgactggcc gggcggcgaa tcgcagcgac cggctccact
     6121 cgtggccggc gagccggcgt cgaaccagct ccgcagatcc tcgctgtccg cgatcccgtc
     6181 aagacaggcg gaagaaagga actcgctgct gagcaggtcc acgaccggcc tgccgttgac
     6241 gatcggggcg gcgaggagct gttcctggct cttgtcaccg ttcagccagc gctcgaacca
     6301 gggcaggacc cagtgggcca gatcaagttt cttgtcggcc gaggcgccat cggtgaaccc
     6361 gccgtgcggg taacccttca tgaccagctg catgttcgga accccgtgtt cggaccacca
     6421 acgggggccg ctcagtttca ggtcggccgg tttgaagtcc ggcttgtagg cgcagggctc
     6481 gtccattgcg aagctgagcg ccggaacgct cggggtgacc tgggtggccg ggacgggatc
     6541 actctggcag tcgatccggg ccgcgccggg atccccctgc acgtatcggc ggagggtgtc
     6601 aaaggcgact atcgccttga tgcccaggga gggatccccc tggagctggc tcgaaatgat
     6661 cgatcccatc gaatgcccgg aaagtccaag ccggcccagg tcgatcatcg gcccgaggtt
     6721 cgggtccgag acctggaggc ctttgaggta cgcgatcacc gagcggactg cgtcgacacc
     6781 gctgcggtag gcatcggaag cggtctgctc gttacccggc aacgagcggt gaaccgtggt
     6841 cacgaagccg tgaccggcca ggtactgggc cgcccaccag agcgcacagc ggttgccacc
     6901 gagcccgtgc tgaaagacga ctaccgggcg gggcccggga taggtcaccg tgtcggcggg
     6961 gcggagcacg gtcatcgggt aagtcgccgg aacctggctg gaatcggcct gatggcgaag
     7021 cgaggggatc gtgaagtcct gcccgaccga agctcccatc cacgaaagcg aattcgaggc
     7081 agaagtcggg cagggattgt caccggtcac cgcagcgtca gcccggccgc cgaaacaggc
     7141 aagggcgacg agcatggcca gcagggcgag gcatctcgcc aggcggggaa gcttgtgatc
     7201 ggcagaggtt ttcacgggta ctaatcccaa caccgagacc gccgcgagtt gcgatccggt
     7261 cgcgcctgcg aaagccgtgc atagaggcag tcgcgaagcc gcggatatgg acaatgggca
     7321 cattcgatat gtcccgtttg gacaaaacgt ggttgcggag gccacccgtt ttcgcatacg
     7381 gttcaaccga acttgccgga ggaggcgttc tgaaactccg gttcttgtcc agctgagaga
     7441 ggggttcgat ggaaggctcg ccagatctac cgccgatcga tccggatgcg gatcagaccg
     7501 aggccgtagc cgggaacgcc gcgagatctg ggagcggggg agttcgcttg gagggagtca
     7561 ccaaaaagtt cggtgatttc acggcagtaa gtgagatgga tctcgacatc gccgacggcg
     7621 agttcttcac catgctcggt ccctccggct gcggcaagac gaccaccctg cggatggtcg
     7681 ccggcttcga acagccgacg acaggcaagg tcctgatcga tggcacggat gtcgccggcc
     7741 aaccggccta caagcgcccg accaacaccg tcttccagtc ctacgcgctt tttccccaca
     7801 tgaccctgga ggagaacgtc gggttcggtc tcaagcgcaa gaaggtgccc aaggccgaaa
     7861 tcaagcagcg tgtagcgaac gaactcgaga gggtcggcct ttcccgcgag gcgaagcgca
     7921 aaccgaatca gctctctggc ggccagcagc agcgcgtcgc cctggcccgc gccctggtca
     7981 acctgcccaa ggtgctcttg ctcgacgagc cgcttggcgc tctcgacctc aaactccgca
     8041 aaggcctcca ggtccagctc aagcagatcc agcgcgacgt cggcatcacc ttcgtctacg
     8101 tcacccatga ccaggaagaa gccctgacca tgagtgaccg gatcgcggtc atgaacgagg
     8161 ggcgcatcga acaatgcgca gatccggaga cggtctacga ccgaccggcc accaccttcg
     8221 tggcgggctt cattggcgtt tccaacctga tgcccgccga agtcaaggga tcgaacgaaa
     8281 tccaggtcgt gaacggcccc ttggtcccga cccccgacgc cgcgaagtac tccggtggcg
     8341 agaagtgttt tgcggtagtg cgtccggaga agctgacggt ttcgctccct gagaacgcgc
     8401 catccgacca gcccggcgtc gacggaatcg tcgaaagctc gctttacctc ggcacctcca
     8461 cccagatggc ggtgcgggtc accgaggacg tgatgatgac cgtccttgtc ccgaacgcgt
     8521 ccgaagatgc ccgacagcac ctcccgggcg gcggcgcaaa ggttcgcctc aactggctgc
     8581 ccgagcacat gcacctcgtg gccgagtccg aatcggccac ggccgccacc gacccaacgc
     8641 ccaccgaaaa agtcaccgaa ggagtcaact gatgtccgag agcaatgtga aacgcgttcg
     8701 actgctggcc ctggccgtcc tggccctggc cgtctcgatg gtcctcgtcg cctgcggcgg
     8761 cggaggcatc gacgaaggct ctgacaactc tgacgtgacc accgcaaccg gcggcgatcc
     8821 gcagggatca ctgaagattt cgaactggcc cctctacatc gacggggcca cgatcaagga
     8881 tttccagaat gacaccggca tcaacacgac ttacacagag gatgtcaacg acaacaacga
     8941 gttcttcggc aaggtccagc agcagctcgc acagggcgac tcgggaggcc gcgacatcat
     9001 cgtcgtgacc gactggatgg ccgagaagat gtacaacctc ggttatatcc agaatctcga
     9061 caaggccaag cttcccaccg tcgagaagaa cctgatcccg agcctccaga gtccgtcgtt
     9121 cgaccccgaa cgcaagtaca gcgtgccgtg gcagtccggc atgaccggcc tggtcgtccg
     9181 caaggatctc gctccggacg tgaaatcgat caatgacctc ttcgacccga agtacaaggg
     9241 caaagtcacc ctgctgaccg agatgcggga tacggtgccc ctggtcatgg ccgccgacgg
     9301 ggtcgacccc aaggaggcga ctacccagga ctggctcgac gcgatcgaca agatcaagga
     9361 gaacgtcgac aacgggcaga tccgccgctt caccggcaac gacttcgtcc aggacctggc
     9421 caagggcgat gtggtcgcgg cgatcggctg gtccggtgac gcggtccagg cccagtccga
     9481 caacgagaac atcgagtacg tccagcccga gcagggctgt tcgatctggt ccgacaacat
     9541 gctgatcccg gttggggcgc ccaacccggc cgcagcctac aagttcatga actacgtcta
     9601 cgagcccgaa aaccaggcgc agatcaccaa gtacgtgaac tacgtaagtc cggtcaaggg
     9661 cgtaagggag atccttgaga agcaggatcc tgcgatcgcc aacaacaacc tgatcttccc
     9721 cagtgagcag ttcaccaaga actgcttcgc gcaggtctca ccgcccggcg acgaagccgc
     9781 gcagcaggaa gtcgagcagg ccttccagga cgtggtcaca ggctgacccc acgaaacagc
     9841 acagaaatca ggatgaagaa gccggcggat caatgagaaa gagttccaac tgatgacgat
     9901 ccgccggcgt ctgattcctt actggctgct cgccccgggc ctgatctggc tcgttctctt
     9961 cttcgtgatc cccatgtatt acatggggga gctctcgctg aagaccggct cgctcgaaac
    10021 cggataccag ctcacctggg cgttcgagaa ctacaccgac tcgatcagcg agtactcgac
    10081 ccagttcatc agggccttct ggtacgccgg cgtcgcgact ttcttttgcc ttctgatcgg
    10141 ctacccgctc gcttacgcga tcgccttcaa ggcggggcgc tggaagaacc tgatgctttt
    10201 cgcggtggtg gcacctttct tcaccaccta cctgatccgg accatcgcct ggaagacgat
    10261 ccttgatgac tccagcccgg tagtcagctt cctcaagacg attcacctcg tgcctcagga
    10321 cgggcgggtc ctggccacat cggctgcggt gatcgccggc ctcacctaca acttcctgcc
    10381 cttcatggtc ctgccgctct tcaccgctct cgagcggctc gaccagcggc tgcttgaggc
    10441 cggcaaggac ctgtatgcgt cagccacgca gacgttctgg cggatcaccc ttccactgac
    10501 catgcccggc atcatcgccg gcatcctctt gactttcatc ccggcggccg gtgactacgt
    10561 caacgcgact tttctcggcg gcaccaacca ggcgatgatc ggcaacgtga tccagtcgaa
    10621 gtacctggtg gtcaacgatt atccggatgc ggcggcgctc tccttcacgc tgatggcgat
    10681 catcctcgtc gcactcctgt tctacataaa gttcgccggt tcggaagcgt tgatgggcga
    10741 agaggaggag acacgatgaa agcctggcgc tggaccaagg atcacatggt gctcttcgtc
    10801 gggttgcttg ccctcgcgta catgctgatc ccgatcctcg tcatcgcggt cttctcgttc
    10861 aacaacccgg agggccgtta caacttcgag tgggtcggct tcaccaccga atactggggc
    10921 aagcccttcg cgatcaccga actgaccgac gcactgctgg tcagtatccg gcttgccttc
    10981 ttcaccgcaa tcggtgccac gatcctcggc actctgatcg caatcgccct ggtccggtac
    11041 cagttcttcg ggcgtcgggc ggcgaacctc ctgatcgtga tcccgatggc gacccccgag
    11101 gtcgtgatcg gtgcgtcgct gctctcgatg ttcctgatct atggcgccaa gctcgggttc
    11161 ggcacgctgc tgatcgccca catcatgttc tcgatcagct tcgtggtgat cgtggtgagg
    11221 tcgcgtctga tcggattcga ccgacggctc gaggaagcgg cggcagacct cggggcgaat
    11281 gccttcacga cttttcgcaa ggtgaccctg ccgctgatcg cgccaggggt catctcggcc
    11341 gccctgcttt ccttcgccct ctcgattgac gacttcgtga tctcgaactt caactcgggc
    11401 tccaccgtga ccttcccgct ctacatcttc ggcgccaacc agcgcggcat cccggtccag
    11461 gtcaacgtga tcgcgaccat gctcttcgcc gtcaccgtga tcgcgatgat cgccgtcatc
    11521 ctgcagcagc gccgggccga gaaggcagcg ggcctgcggc cggacgagat cgccgacaag
    11581 aaagccatct ccctccccgt ctgaccctgt tggtgaccac gccgcgggcc cgagtgggcc
    11641 ccggtggtta cgccgtggga tcgggtggtt acgccgcggg ggccggtggt tacgccgcgg
    11701 ggtcgggtgg gggtagccgg gacggacgca cgcgcggcat tcagccgcgc tgg
//
LOCUS       DAOQKN010000069        11701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_2571_length_11701_cov_5.417912, whole genome shotgun sequence.
ACCESSION   DAOQKN010000069 DAOQKN010000000
VERSION     DAOQKN010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 11701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11701
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2571_length_11701_cov_5.417912"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="PLJ64_06270"
     CDS             complement(<1..987)
                     /locus_tag="PLJ64_06270"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNC93120.1"
                     /translation="MARTVILSTARTPFGKMGGGLATVNAAELGGVAIAGALERAGVA
                     PDQVQQVSYGQVIQAGQGQIPSRQAQINGGIPKEVPSETVNKVCASGMRAAGLIDQAI
                     RAGDIEVGVAGGMESMSLAPYLLPGARSGFRMGDVKAVDSMTHDGLTNPFTHLQMIAE
                     ASTVSNEVGISREEMDAWSARSHELAQKAKEAGVLAEEIVPVTVKGRKGDTVVDEDEA
                     IRPGTTAESLSGLRAVGGEDATHTAGNAPGVNDGAGALVLASEEWAEANGKTPMATIV
                     GYGQIGDEYACLAKTPALAAKAALDRLGKTPADVDLWEVNEAFASVTLNTIKM"
     gene            1194..3530
                     /locus_tag="PLJ64_06275"
     CDS             1194..3530
                     /locus_tag="PLJ64_06275"
                     /inference="COORDINATES: protein motif:HMM:TIGR04534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93121.1"
                     /translation="MAGEVSAGPVSTYTTGPVADINPGAGSGISNSVDPEFTAAGNRV
                     FFVADDGVNGKEVWVSDGRPGGSTQMAKDINPAPGVGSNPRDLVTYQGEVYFSADDGA
                     SGRELWKSDGTEAGTVLLKDIQPGTDPSLPGRPVEAGGKLFFAANEGPGPTFRGNELW
                     VTDGTGPGTVMVRDIEADGCLGGSSPRELTEVGGLLYFVANFSSPGCSDSSLGELWKS
                     DGTEAGTERVSDLNGPGDASDPFITSITALNGLAVFAADLPATGQELFASDGTDFGTN
                     LVMDIKPGSGNGVMSRLAAFNGWVYFQGNDSTNRELWRSNVTSTTELFKDVDPAGQSQ
                     PDGFTVANGALWFDAFVTGLSDEPWVSDGTPGGTNLVKDINTSGNSYPEDFTGLGDRT
                     FFLASDTSSTPFTMTNKQLWQSDGTAAGTFARSSIAPGDPIENKVRWLTKAGPRLIFA
                     ADNNDGKGFELWSFADNDPPETSITSGPASNSTTLDVTPEFGFDSDDLPATFECRLFN
                     NNIDPVPPFGACSGPGSTHSPASPLADGAYRFEVRATDESGNVDPTPVKRGFIIDGTA
                     PQTEIDSGPVEGSTITGTSPTFGFSSPGSSGTSFECRLFASDQAPPSFSACSGPGQTH
                     TPAAPLAEGTFTFEVRATDPVGNTDQTPASRTFTVKLETTTDMTVAGAASARKTQKQR
                     GRKVGVAVRVKAGEDLRITLSGKAIDGRRKLGLRKVTGSMKADGSKTIVLRLARKQAN
                     RPLLARIKRTRKVRALVMVTLSDQAGNRKRIRLPVKLR"
     gene            3563..5008
                     /locus_tag="PLJ64_06280"
     CDS             3563..5008
                     /locus_tag="PLJ64_06280"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HNC93122.1"
                     /translation="MKAYTTDVALSDLDADLVAVGLFEGDGLSEPLGSTAGASDASGD
                     FKSKVIVYPGKPERVVIIGLGPKDGFTLEKARVAGAVAQKALKEVKGEALAWMLPDTG
                     DLDPGGVAACLVEAAIFSAFDFDRYKSGSDGKEAPPSLKAVEINTGKDVAAMVKVGEA
                     VGNAGNRARYLQSLPANVATPEYLAGRAREIAEAHEKVTVEVMDRAAIIKAGMGGLEA
                     VSKGGEHVEPRLITLRYTGKGGGETLGLVGKSVTFDTGGISIKPSAGMHEMKMDMSGG
                     AAVLEGLNAIAELEIPLDIIAVLPATENMPSGTALKPGDIITQLNGKTVEVTNTDAEG
                     RLILADALVHCAREGADRLVDLATLTGAVLIGLGSTYAALISNDDDLAAAVEKAGELS
                     GELVWRLPLHDEYKDLTKGEITDLVNASAQRKAGTIYAGSFLEEFTEGKPWAHLDIAG
                     TAWDTGREYWGKGPTGFGVHLLVALAYDLSS"
     gene            complement(5015..5563)
                     /locus_tag="PLJ64_06285"
     CDS             complement(5015..5563)
                     /locus_tag="PLJ64_06285"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNC93123.1"
                     /translation="MKPGPGNARERAAEIAVEKPGISRSLTLDWQDPTASAAEGSKLA
                     GLEYMVAVMDGTIPPAPIAVLMNMTPTEVEEGRAVFFALPGEEHYNPIGVVHGGFMAT
                     LLDSVAGCAVHTTLALGEAYTTLTLEVKNLRALTRDTGSVRAEGEVTYRGRRQATADA
                     KLYAEESGKLLASATSTCLIMA"
     gene            5846..6994
                     /locus_tag="PLJ64_06290"
     CDS             5846..6994
                     /locus_tag="PLJ64_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNC93124.1"
                     /translation="MNFDLTDEQRLLQNTVRDFARNEVVPVAEELDRTKAFPYEIVAK
                     LGDLGLMGIPFPEEYGGSGGDILSYALAVEELARIDSSVAITMAAHISLGTTPIYNWG
                     TDEQKADWMPELTAGRKLAAFGLTEPEAGSDAGNTKTRAELDGDEWVVNGAKQFITNS
                     GTDISACVTITAVTGKDENGRPEISNIIVPNGTPGYTVDPPYRKMGWNASDTHPLTFE
                     DARVPESNLLGPRGQGFRQFLQILDGGRIGVAAMSVGVAQGALDEAIKYAKERQAFGQ
                     SISKFQSIQAKIADISAQLEAARLLTYRAAIEKDRGENFSLTAAQAKLITGRLAVRAT
                     EEAVQIHGGYGFIEEYPVCRFYRDAKILTIGEGTDEVQQMVIARALGC"
     gene            complement(7016..8020)
                     /locus_tag="PLJ64_06295"
     CDS             complement(7016..8020)
                     /locus_tag="PLJ64_06295"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNC93125.1"
                     /translation="MERRTGIREIAREAGVSAATVSLALRGKGRMSEATRDRIKEIAR
                     AHDYHPNAAARSLTRGRTGLIAISMPGTGGAPEVLGSVQYFFRLLGAAAARALELEYG
                     LLVADPRKGAGQTALDGAIVVDPAIDDPVVAACDTAGLPVVTIGRRLASDESGKAGTW
                     VVDNDFLAATGSVLDHLAGQGARSIALMASEPIDSFQQDSIDAYRQWCAGHGVEARVV
                     VSQSPDPKDATVAAGRLFDQGQMPDGVYATIDTLARALLDRAASERVAVPGELRIATC
                     SNGELARTSQPQLTTLDEHPATLGEEAVELLVRAIEDPESAPAFVKVDTELLVRESTA
                     "
     gene            8143..9954
                     /locus_tag="PLJ64_06300"
     CDS             8143..9954
                     /locus_tag="PLJ64_06300"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNC93126.1"
                     /translation="MRTGILKLTLALAGIGLGLAAPVTANAAPAADTVFRNGTVITID
                     RAQPKAQAVAVVGGRIAYVGSDRGAGKWIGKGTRVVNLKGRTMMPGLVDGHSHPLGGG
                     DILDDCDLGNIEATVEDMLDIMRDCDAADPATSNSDWLQVSNWSPVGVLPAGTVVTKA
                     DLDAAFPDRPVYVQGSDFHNSWVNSRALELAGVTKDTPDPATGEFVRDDEGNPTGLLK
                     DTAQDVVKSAIPPKSFKESLADGRRAIKAITAVGITSATDAAADPGTLKVWKGLASKG
                     QMPIRLNSFVVVDAGQGVHGAAAYYKAMEKKYSTERLRVPGVKMFMDGVIEYPAQTAA
                     LIDPYLEKVGDEWVPGSNRGDIYFTQKQANRFVTRFDRMNRRIHVHAIGDRAAKVALN
                     AAAAARKANRSARRDNIAIAHLQLVDPSDYGRFAKLGVYADMQLQWAVSDFWTEDALH
                     PFIGDERYNRLYPARSLVKAGAPFAMGSDWPVDPLNPWNEIMTANTRRNDYGGLLLAN
                     QSIPVATALRAHTLGSARQLGISGKVGSVTVGKDADLIVINRNPLKARPGSIDKTRVL
                     RTIIRGRTVYTPGPGSSALISRAASLSTGEAGHDHGH"
     gene            complement(10039..10683)
                     /locus_tag="PLJ64_06305"
     CDS             complement(10039..10683)
                     /locus_tag="PLJ64_06305"
                     /inference="COORDINATES: protein motif:HMM:NF028169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC93127.1"
                     /translation="MTIHGRRIEIIRALRELQNPRIPDEGRRDLAARDAILDSTVELV
                     LSEGWEALTIEGIANRARVSKQTIYRWWKSKGAVLMDAVLANGFKWPAIPDTGDFEAD
                     LKGGLRSLVGEFSDASFDSLLRALLIGAQEDGKLSAELDRQVRSWITAEFAFLIESAR
                     QEGRIEAHADPELIIELLYGAVFTRWLLRTGSLDFRFADAVAEMTARALTPGRS"
     gene            complement(11044..11445)
                     /locus_tag="PLJ64_06310"
     CDS             complement(11044..11445)
                     /locus_tag="PLJ64_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93128.1"
                     /translation="MSEKSSPQPSLEDIKAWEGYRLDDMTGQGVARVEGLFVDGESGQ
                     PTWVLVKLGRFGKVVPISIRECAAAAGRVWTPYDREVIRNAPAVDPALPLNREQEQQV
                     LEYYGIPETVGRGAELKDRPAGSTTANPITT"
     gene            complement(11438..>11701)
                     /locus_tag="PLJ64_06315"
     CDS             complement(11438..>11701)
                     /locus_tag="PLJ64_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HNC93129.1"
                     /translation="RGEGIEELAAKVAEHRAFIENEGTLAERRARNLRSEVLGLATAR
                     LRRRLEAAIEDDPRVVELLNRVVSRELDPASAAAELLEGEIDE"
BASE COUNT         2075 a         3980 c         3720 g         1926 t
ORIGIN      
        1 catcttgatc gtgttaaggg tgaccgaagc gaaggcctcg ttcacctccc agaggtcgac
       61 gtcggccggg gtcttgccga gcctgtccag cgccgccttt gcggcgagag ccggggtctt
      121 ggcgaggcag gcgtactcgt caccgatctg gccgtaaccg acgatggtcg ccatcggggt
      181 cttgccgttg gcctcggccc actcctcgga ggccagcacg agcgccccgg cgccgtcgtt
      241 cacgcccggg gcgttgccgg cggtgtgggt ggcgtcttcg ccgccgaccg cacgaaggcc
      301 ggagaggctc tcggcggtgg tgccggggcg gatcgcctcg tcttcgtcga ccacggtgtc
      361 gcccttgcgg cccttgaccg tgaccggcac gatctcctcg gccagcactc cggcttcctt
      421 cgccttctgg gccagctcat gcgagcgggc cgaccatgcg tccatctcct cgcggctgat
      481 gcccacttca ttcgaaacgg tcgaggcctc ggcgatcatc tgcaggtgag tgaagggatt
      541 ggtcaggccg tcatgggtca tcgagtcgac cgccttgacg tcacccatgc ggaaaccgga
      601 ccgggcaccg ggcagaaggt acggcgcgag cgacatcgat tccatgccgc cggcgacgcc
      661 aacctcgatg tcaccggcgc gaatcgcctg gtcgatcagg ccggcagccc gcatgccgga
      721 ggcacagacc ttgttcacgg tctcggaagg gacttccttc gggatgccac cgttgatttg
      781 cgcctggcgg gaagggatct ggccctggcc ggcctgaatc acctggccgt agctgacctg
      841 ctggacctgg tccggggcga ctccggcgcg ctcgagggcg ccggcgatcg ccacaccgcc
      901 gagttcggcc gcattcacgg tggcgagtcc accgcccatc ttgccgaagg gggtgcgggc
      961 agtgctcagg atgacggtgc gggccatggt gcctttcagg tctcgagggg aaatcggaaa
     1021 acttcggacg aaacgaagcg gaatatggaa gataccgggt gccccttacc gggggcctct
     1081 gcttcggtta ggcttgactc aatccaggcg aacgggggtt ccgggcagtg gggagtttca
     1141 gactgaggag tgtctcgctt gtctttgtgc tgtttgcgtg gggatccttg ctgatggctg
     1201 gcgaggtcag cgcgggaccg gtctcgacct acacgaccgg gccggtggcg gacataaacc
     1261 cgggtgccgg cagcggtatc tcgaactcgg tcgacccgga attcaccgcg gccggaaacc
     1321 gggtcttctt cgtggccgat gacggggtga acgggaaaga agtctgggtc agcgacggca
     1381 ggccgggcgg aagcacccag atggccaagg acataaaccc ggcccccggg gtcggcagca
     1441 accctcggga tctcgtcacg taccagggcg aggtctactt ctcggccgac gacggagcga
     1501 gcggccggga actctggaaa tcggacggga ccgaggccgg aacggtgctg ctcaaggaca
     1561 tccagcccgg taccgatcca tcgttgccgg gcaggccggt cgaagccggc ggcaaactct
     1621 ttttcgccgc caacgaaggc cccggtccga ccttcagggg gaacgaactc tgggtaaccg
     1681 atggcacggg cccgggaact gtgatggtca gggacatcga ggccgacggc tgtctgggtg
     1741 ggtcgagccc gagagaactg accgaagtcg gcgggcttct ctactttgtc gccaacttct
     1801 cgagtccggg ctgcagcgat tccagcctgg gcgagctctg gaagagcgac ggcaccgagg
     1861 ccggaaccga acgggtctcg gacctgaacg ggccgggtga cgcctcggac ccgttcataa
     1921 cctcgataac cgcactgaac ggactggcgg tattcgccgc tgatctgccg gcaaccggcc
     1981 aggagctttt cgcaagcgac ggcaccgact tcggcaccaa cctggtcatg gacatcaaac
     2041 ccggttcggg gaatggcgtg atgagtcgcc ttgcggcctt caacggctgg gtctacttcc
     2101 agggaaacga ctcgaccaac cgcgaactct ggcgatcaaa cgtgaccagt acaaccgaac
     2161 ttttcaagga tgtcgacccg gccggtcaga gccagcccga cgggttcacc gtggcgaacg
     2221 gggcactgtg gtttgacgcc ttcgtcaccg gactctccga cgagccctgg gtaagcgacg
     2281 gcacccccgg cggtacgaat ctggtcaagg acatcaacac ctcgggcaac tcgtatccgg
     2341 aggacttcac agggctgggg gaccggacct tcttcctggc cagcgacacg tcgagcacgc
     2401 ccttcaccat gaccaacaaa cagctctggc agtcggatgg caccgccgcc gggaccttcg
     2461 ccagaagctc catcgctccc ggggacccga tcgagaacaa ggtcaggtgg ctgaccaaag
     2521 cgggcccgcg gctcatcttc gccgccgaca acaacgacgg caaaggcttc gagctttgga
     2581 gcttcgccga caacgatccc ccggagacgt cgatcacctc ggggccggcg agcaattcga
     2641 ccaccctgga cgtcacgccc gaattcggat tcgattccga cgatctgccg gccactttcg
     2701 agtgccgcct cttcaacaac aacatcgacc ctgtgcctcc cttcggtgcc tgctccgggc
     2761 ctggatcgac tcacagcccg gcgtctccgc tcgccgacgg cgcctatcgc ttcgaagtcc
     2821 gcgcgactga cgaatccggg aacgtcgacc cgaccccggt caagcgaggc ttcatcatcg
     2881 acgggaccgc accccagacc gagattgatt ccggcccggt cgagggatcg accatcaccg
     2941 ggacctcccc caccttcgga ttcagttcgc ccgggtcatc cggcaccagc ttcgaatgcc
     3001 gtctcttcgc ctcggaccag gcgccgccat cgttctcggc ctgttccggc ccggggcaga
     3061 cccacacgcc ggcggccccg cttgccgagg ggactttcac cttcgaggtc cgggccactg
     3121 acccggtcgg aaacaccgac cagacgccgg cctcacgcac cttcacggtc aaactcgaaa
     3181 ccaccaccga catgaccgtg gcgggcgcgg cttcagccag aaagacccag aagcaacgcg
     3241 gcaggaaggt cggggtcgcc gtgagggtca aagccggcga ggacctgcgg atcaccctga
     3301 gcggcaaggc catcgacggt cgcaggaagc tgggcctgag gaaggtgacc ggttccatga
     3361 aagcggacgg ttcgaagacg atcgtcctgc gcctggccag gaaacaggca aaccggccgc
     3421 tgcttgcccg gatcaaacgg acccggaagg tccgggcgct ggtcatggtc acgctgagcg
     3481 accaggccgg gaacaggaag cggatccgcc tcccggtcaa gctccgctga ccgccgcatc
     3541 cgcgggtctt agactccggc gaatgaaggc ttacacgacc gatgtcgccc tgtccgatct
     3601 cgacgccgat ctcgtcgcgg tcggcctctt cgaaggtgac gggctttccg agccgctcgg
     3661 cagcaccgcc ggagcctcgg atgcttccgg tgacttcaag tcgaaggtga tcgtctaccc
     3721 gggcaagccg gagcgggtgg tgatcatcgg cctcggccct aaggacgggt tcacgctgga
     3781 gaaagcccgg gtggccggag cggtcgccca aaaggctttg aaggaagtca agggcgaagc
     3841 actggcctgg atgctgcccg acaccggtga tctcgacccg ggcggggtcg ccgcctgcct
     3901 ggtcgaagcg gcgatcttca gcgcctttga cttcgaccgc tacaagtcgg gaagtgacgg
     3961 caaggaggcc ccgccctcac tgaaggccgt cgagatcaac accggcaagg acgtggccgc
     4021 gatggtcaag gtcggggaag ccgtcggcaa cgcgggaaac cgggcccgct acctgcagag
     4081 cctgccggcc aacgtggcga cccccgagta cctggccggc cgggcccgcg agatcgccga
     4141 agcgcacgag aaggtaacgg tcgaagtaat ggaccgcgcg gcgatcatca aggccggcat
     4201 gggtggcctc gaggccgtct ccaagggcgg cgagcacgtc gagccgcgcc tgatcactct
     4261 ccgttacacc ggcaagggcg ggggcgagac gctcggcctg gtcggcaagt cggtcacctt
     4321 cgacaccggc ggcatctcga tcaagccctc ggccggaatg cacgagatga agatggacat
     4381 gtccggcggc gccgcggtgc tcgagggcct gaacgcgatt gccgagctgg agattccgct
     4441 cgacatcatc gcggtcctcc cggcgaccga gaacatgccg tccggaacgg ccctcaagcc
     4501 gggcgacatc atcacccagc tcaatggcaa gacggtcgag gtgaccaaca ccgacgccga
     4561 gggccggctg atcctggccg atgccctggt ccactgcgcc cgcgaaggcg ccgaccgcct
     4621 ggtcgacctt gcgacgctga ccggcgcggt gctgatcggc ctcggctcga cctacgcggc
     4681 cctgatctcg aatgacgacg acctcgccgc agccgtcgag aaggcgggcg agctgagtgg
     4741 cgaactggtc tggcgcctgc cgctgcacga cgagtacaag gacctgacca agggcgagat
     4801 caccgatctg gtcaacgcct ccgcccagcg caaggccggc acgatctacg ccggatcatt
     4861 cctcgaggag ttcaccgagg gcaagccctg ggcccacctg gacatcgccg gcaccgcctg
     4921 ggacaccggc cgcgagtact ggggcaaggg cccgaccggt ttcggcgttc acctgctggt
     4981 cgccctcgcg tacgacctca gcagctgatc cgggtcacgc catgatcaga caagtcgagg
     5041 tggctgacgc gagcagcttg ccactttcct cggcgtagag cttcgcgtcg gcagtcgcct
     5101 gccgccggcc ccggtaggtg acctcaccct cggcccgcac gctgcccgtg tcgcgggtca
     5161 gcgcacgcag gttcttcact tccagggtga gagtcgtgta ggcctcgccc agcgccagcg
     5221 tcgtgtgaac tgcgcaaccc gcgactgaat cgagcagggt cgccatgaag ccgccgtgaa
     5281 cgacgccgat cgggttgtag tgctcctccc ccggcagggc gaagaacacg gcgcggccct
     5341 cttcaacctc ggtcggtgtc atgttcatga gcaccgcaat cggcgcgggt ggaatggttc
     5401 cgtccatgac cgcgaccatg tattcgaggc cggccagctt gctgccttcc gccgccgagg
     5461 ccgttgggtc ctgccagtcg agggtgagcg aacggctgat cccgggcttc tcaaccgcga
     5521 tctccgccgc ccgctccctc gcgttaccgg ggccgggttt caccgggtcg gcctccagcg
     5581 tcggggtgcg ccctccggcc tgatcttccc gccaaccccg tactgcaggt cggcgaggat
     5641 ctctgcctct gtgaggtcga tcttcagggg cctcatctgg aatctcgttc ggtttctcgg
     5701 attgcgcttc attcttgctc ccctttcggg gtcggttcgg ccctccgtag agggtcgcgt
     5761 ctgcagtcca ccatatacgt gtaagtctga tcataagact tagatgagat atctcacacg
     5821 aggaaggaat tcgtaggatc gcgtcatgaa cttcgacctg accgacgaac agcgtctcct
     5881 ccagaacacc gtccgcgatt tcgcccgtaa tgaagttgtt cccgtcgcgg aggagctcga
     5941 ccgcaccaag gccttcccct acgagatcgt cgccaagctc ggcgacctcg ggctgatggg
     6001 tatccccttc ccggaggaat acgggggcag cggcggcgac attctttcct acgccctcgc
     6061 ggtcgaggaa ctggcccgga tcgactcgtc ggttgcgatc accatggcgg cccacatctc
     6121 gctcggtacc accccgatct acaactgggg gaccgacgag cagaaggccg attggatgcc
     6181 cgagctcacc gccggccgga agctcgccgc cttcggcctg accgaacccg aagccggctc
     6241 cgacgcagga aacaccaaga cccgggccga gctcgacggc gacgaatggg tcgtgaacgg
     6301 ggccaagcag ttcattacaa actccggcac cgacatctcc gcctgcgtca cgatcaccgc
     6361 cgtgaccggc aaggacgaaa acggccggcc cgagatctcg aacatcatcg tgcccaacgg
     6421 cactcccggt tacaccgtcg atcctcccta ccgcaagatg ggctggaacg cctcggacac
     6481 ccaccccctg accttcgagg atgcccgggt tcccgaatcg aacctgctcg gcccgcgcgg
     6541 ccagggtttc aggcagttcc tgcagattct cgacggcggc cggatcggcg tcgccgcgat
     6601 gtcggtcgga gtcgcccagg gtgcgctgga cgaagcgatc aagtacgcga aggaacgcca
     6661 ggctttcggc cagtcgattt ccaagttcca gtcgatccag gcgaaaatcg ccgacatctc
     6721 cgcccagctc gaggccgccc ggttgctgac ctaccgggcc gcgatcgaaa aggaccgcgg
     6781 cgagaacttc tccctgaccg ccgcccaggc caagctgatc accggccgcc tcgccgtgcg
     6841 ggcgaccgag gaggcggttc agatccacgg cggttacggc ttcatcgagg agtatccggt
     6901 ctgccgcttc taccgcgacg cgaagatcct cacgatcggc gaggggaccg acgaggtcca
     6961 gcagatggtg atcgcccggg ccctgggctg ctgaggcaac cgggtcgggc ttcgctcagg
     7021 cggtcgattc cctgaccagg agttcggtgt ccaccttcac gaaggcgggc gccgactccg
     7081 ggtcttcgat cgcgcggacc agcaattcga ccgcttcctc cccgagcgtg gccggatgct
     7141 cgtccagcgt ggtcagctgg ggctgcgagg tgcgggccag ctcgccgttg ctgcaggtgg
     7201 cgatcctgag ctcgccgggc accgcgacgc gttctgaagc cgcccggtca agcagggccc
     7261 gggcgagagt gtcgatcgtc gcgtagaccc cgtccggcat ttgcccctgg tcaaatagcc
     7321 ggcccgccgc gacggttgcg tccttcgggt ccggcgactg cgagacgacg acccgggcct
     7381 cgaccccgtg cccggcgcac cactgccggt aggcatcgat gctgtcctgc tgaaaggagt
     7441 cgatcggctc gctggccatc agggcgatcg accttgcgcc ctggccggcc aggtgatcga
     7501 gcaccgaacc ggtggcggca aggaaatcgt tgtcaaccac ccaggtaccg gcttttccgg
     7561 attcatcgga ggccagccgc ctgccgatcg tgaccacggg caggccggcc gtgtcgcagg
     7621 cggccactac ggggtcatcg atcgccggat cgaccacgat cgcgccatcg agtgcggtct
     7681 ggccggctcc ctttcgggga tcggcaacca gcaacccgta ttcgagctcg agcgcgcgag
     7741 ccgccgcggc gcccagcagc cggaagaagt actggaccga ccccagcact tccggcgccc
     7801 cgccggttcc gggcatggat atcgcgatca gcccggtccg cccccgggtc agtgacctcg
     7861 ctgccgcatt cgggtggtag tcgtgggccc tggcgatctc ctttatccgg tccctggtcg
     7921 cctccgacat ccgtcccttc ccccgcaggg caagtgaaac cgtcgctgcc gaaacccccg
     7981 cctccctggc gatttcacgg attccggtcc ggcgttccat cggcgaagca tagtcaggaa
     8041 cacccacgga accgcgaatc tgcgctacat tggttaagcg cttaatcatc tttgatcaag
     8101 ccaatctcgt caaacagcag caggatggag gcaggaaccc gcatgaggac tggaattctc
     8161 aaattgaccc tcgccctggc cggcatcggc ctcggactgg ccgccccggt aaccgcgaac
     8221 gccgccccgg ccgcggacac ggtcttccgg aacggcaccg tgatcacgat cgaccgggca
     8281 caaccgaagg cccaggccgt cgccgtcgtg ggcggccgca tcgcctatgt cgggtccgac
     8341 cgcggagccg gcaagtggat cggcaaaggc accagggtgg tcaacctcaa gggccggacc
     8401 atgatgccgg gcctggtcga cgggcattcc catccgctcg gcggtggcga catcctcgac
     8461 gactgtgacc tcgggaacat cgaagccacg gtcgaagaca tgctggacat catgcgcgac
     8521 tgtgacgccg ccgatccggc caccagcaac tcggactggc ttcaggtctc caactggagt
     8581 ccggtcggcg tgctccccgc cggcaccgtg gtgaccaaag ccgatctcga cgctgccttc
     8641 ccggaccggc cggtctacgt ccagggttcg gacttccaca actcatgggt gaattcgcgg
     8701 gccctcgagc tggccggcgt gaccaaagac acgcccgacc ccgcgaccgg tgagtttgtc
     8761 cgcgacgacg aaggcaatcc gaccggcctc ctcaaggaca cggcccagga tgtggtcaag
     8821 tcggccattc cgcccaagtc gttcaaggaa tccctcgccg atggccgccg ggcgattaag
     8881 gcaatcaccg cagtcggcat tacctcggcc accgacgccg ccgccgaccc gggcaccctg
     8941 aaggtgtgga agggcctggc cagcaagggc cagatgccga tccgcctcaa ttccttcgtt
     9001 gtggtcgatg ccggccaggg cgtacacgga gccgccgcct actacaaggc gatggagaag
     9061 aagtactcca ccgaacgcct ccgggtgccc ggcgtgaaga tgttcatgga cggagtgatc
     9121 gagtatcccg cccagaccgc cgccctgatc gatccctacc tcgagaaggt cggtgacgaa
     9181 tgggttccgg gcagcaaccg cggcgacatc tacttcacgc agaagcaggc caaccggttc
     9241 gtgacccgct tcgaccggat gaaccggcgg atccacgttc acgcgatcgg cgaccgggcg
     9301 gcaaaggttg ccctgaacgc cgccgccgcg gcccgcaagg ccaatcgttc cgcccgccgg
     9361 gacaacatcg cgatcgccca cctccagctg gtcgacccgt ccgattacgg acgcttcgca
     9421 aagctcggtg tctacgccga catgcagctg cagtgggcgg taagcgactt ctggaccgaa
     9481 gacgccctgc atccgttcat cggagacgag cgctacaacc gcctctaccc cgcccgcagc
     9541 ctggtcaagg caggggcgcc gttcgcgatg ggcagcgact ggccggttga tccgctcaat
     9601 ccctggaacg agatcatgac cgccaacacc cgcaggaatg attacggcgg actgttgctg
     9661 gccaaccagt cgattccggt ggccaccgcc ctgcgggctc acaccctggg ctcggcccgg
     9721 cagctcggca tttccggcaa ggtcggttcg gtcaccgtgg gcaaggacgc ggacctgatc
     9781 gtgatcaatc gcaatccgct caaggccagg ccgggcagca tcgacaagac ccgggtcctc
     9841 aggacgatca tccggggcag gaccgtctac acgccgggcc ccggttcatc cgccctgatc
     9901 tccagggccg ccagcctcag caccggcgaa gccggccacg accacggcca ctaacccgac
     9961 caggtcctga cccgaccagg gcctgagccg accgcggcct gagtcgacca ggtcctgagt
    10021 cgaccagatc cgtttggttc atgaccgccc gggtgtcaaa gcccgggcgg tcatctccgc
    10081 caccgcgtcc gcgaacctga agtcgaggct gccggtccgc agcagccatc gcgtgaacac
    10141 ggccccgtag agcaattcga ttatcagctc cgggtccgcg tgagcttcga ttcgcccctc
    10201 ctggcgggcc gactcgatca agaacgcaaa ttcagcggtg atccaggacc gaacctgccg
    10261 gtcaagttca gcggagagct tcccgtcctc ctgggcgccg atcaacaagg cacgcagcag
    10321 ggaatcgaag ctggcgtcgg agaactcacc gaccaggcta cgcaggcctc ctttgaggtc
    10381 ggcttcgaaa tcacccgtat ccggaatcgc cggccacttg aagccattcg cgagtacggc
    10441 atccatcagc acggcgccct ttgacttcca ccaccggtag atggtttgtt tggatacccg
    10501 tgcccggttg gcgatgcctt cgatcgtcag agcctcccag ccttcggaaa gaacgagctc
    10561 caccgtggag tcgagaatcg cgtcacgggc agcaaggtcg cgtcggcctt cgtcggggat
    10621 ccgcgggttc tgaagttcgc gaagtgccct gatgatctcg attctccgac cgtggattgt
    10681 catcccggac ttcagtaacc gaaacgcaaa actctccccc gtcagaccaa cccgacccga
    10741 cgggacccgg tcggaccgga cccaaccggt cgggcccgca acacatcgaa aaaaacactg
    10801 gcgagacgca acgtggcgtc tcgcggggac ttgacaaaac gtggtgatcg gggttcggca
    10861 gcccgaggat ttgacaactc gtcaaatctc aacgagacgc aacgttgcgt ctcgccacgg
    10921 gatttccggg cctgcctggg gcctcggttg gtttcccgac ctgcctggcc tcgattgctt
    10981 tccgggcctg atgacctcgg ttgtcttccg ggcctgcctg gcctcgattg cttggggttc
    11041 gggtcaggtg gtgatcgggt ttgcggtggt tgagccggcg gggcggtcct tgagttcggc
    11101 tccgcggccc accgtttccg ggattccgta gtactcgagt acctgctgct cctgttcacg
    11161 attgagcggg agggcggggt cgaccgcggg cgcgttgcga atgacttcgc ggtcgtaggg
    11221 agtccagacc cggccggcgg cagccgcgca ttcgcgaatc gagatcggga ccaccttgcc
    11281 gaagcggccc agtttgacca gcacccaggt cggctggccg gactcgccgt cgacgaacag
    11341 accttcgacc cgggccacgc cctggccggt catgtcatcc agccggtaac cctcccaggc
    11401 cttgatatcc tcgagactcg gttgcgggga agacttctca ctcatcgatt tctccttcca
    11461 gcagttcggc cgcggccgac gccgggtcca gttcccggct gaccactcgg ttcaacaact
    11521 ccaccacacg gggatcatct tcgattgccg cttcgagccg ccgacgcaac ctggccgtgg
    11581 ccagccccag cacctccgaa cgcagattgc gggcccgcct ttcggccagc gtcccttcat
    11641 tctcgatgaa ggcgcggtgt tccgccactt ttgcggcaag ttcttcgatt ccctcgccgc
    11701 g
//
LOCUS       DAOQKN010000070        11517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_2627_length_11517_cov_5.755540, whole genome shotgun sequence.
ACCESSION   DAOQKN010000070 DAOQKN010000000
VERSION     DAOQKN010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 11517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11517
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2627_length_11517_cov_5.755540"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1219)
                     /locus_tag="PLJ64_06320"
     CDS             complement(<1..1219)
                     /locus_tag="PLJ64_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93130.1"
                     /translation="MISGARQIVRLAAIFLAMSAALAFGASGAQAASINVNSAFPYIT
                     GNDGWGLSGDASANFLVYEPTVNVYAYKLPYSFGDPIVSNGTGNVEAFTGDWSLTMHN
                     LGTTHILPEGKYRLDMIQDPLLPYKTGGMASIGADAVIYVDRTGPQTSINSVVPNPTA
                     STNANFTFSANDFNAAFSETGSGVASFQCSKDGGAWFTCGNDDAFASIGEITYVAPEG
                     IRNFRVRAIDRLGNIGDPASFDWLVDLTDPEIHLPAISQDANQNVVPAYVLGENPPAS
                     GTCSDPLAGSPAGASGIRSCVVDPVSTSQLGNFSRAASAEDKVGHTASTHGAYRVVPP
                     RYRDLVAQDDPIAWYRLDDPLGSSTLIDSSGHNHNGDAKNGLVLDRAPALACHQVVRP
                     NGCEVAHDIRGRAA"
     gene            complement(1249..1704)
                     /locus_tag="PLJ64_06325"
     CDS             complement(1249..1704)
                     /locus_tag="PLJ64_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtoQ family protein"
                     /protein_id="HNC93131.1"
                     /translation="MSERTWRVYLAGEIHSDWRDRVEAACEGLPVVFTAPVTDHPASD
                     AAGTSTLGDEEKKFWADHKGAGVNSIRTQTFIQEADVVVVRFGDKYRQWNAAFDAGFA
                     SALGKPVITQHDEELDHALKEVDAAARATARTPEQVAAVLRYVITAELA"
     gene            complement(1740..2004)
                     /gene="ffs"
                     /locus_tag="PLJ64_06330"
     ncRNA           complement(1740..2004)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLJ64_06330"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(2037..2870)
                     /locus_tag="PLJ64_06335"
     CDS             complement(2037..2870)
                     /locus_tag="PLJ64_06335"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNC93132.1"
                     /translation="MRRTVALLVVLIALGIVAWAALNVSRLWKTDTHGAELTETSIES
                     KAVPGEHPVSVVVPDGADDGERGMLVFLHGRSGDDGSYTDDEEFFEALAGQGGDAPIV
                     VFPDGGESSYWHDRDTGNWGSYVTDEVIPQMAGKFGADPDRVAIGGISMGGFGALDLA
                     IQNPGMFCAVGGHSPALFLSGGESAPGAFDDAEDFDDNDVLATAAADPDAFSSQPLFL
                     DAGKSDPFVPGVTELANSLEAAGVDATVKLNWPGGHDEDYWNAHWDEYLAFYADALAD
                     C"
     gene            complement(2931..3740)
                     /locus_tag="PLJ64_06340"
     CDS             complement(2931..3740)
                     /locus_tag="PLJ64_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93133.1"
                     /translation="MRGRLKELWGKPGFANVVALVALFIALGGVSYAAVQIPKNSVGT
                     KQLKKNAVNSAKVRNRSLLAADFKKGQLPRGATGAAGVTGSTGATGPAGTTGLTGAAG
                     PTGSAGEAGTTGATGTAGATGPTGPRRERSSHDRRPSTTAALGAVASLGSTTQLFSPL
                     GLSTPVSSSSALQVATRAPGVNVKLDNLSVLFDTPTGGDGSVFRTISIGTVDQAGTVT
                     GILGCSVNGGGKICSNSNEVSVPSGSLLVIRSAVSGTPPATMVHVGYTLGP"
     gene            3829..3900
                     /locus_tag="PLJ64_06345"
     tRNA            3829..3900
                     /locus_tag="PLJ64_06345"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3861..3863,aa:Val,seq:cac)
     gene            4034..4435
                     /locus_tag="PLJ64_06350"
     CDS             4034..4435
                     /locus_tag="PLJ64_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNC93134.1"
                     /translation="MSDSATTVNNLGVAMFTVASQDDAIDFYTGKLGWELRGDQAFGE
                     DGSMRWVEVAPPGSGARLALNPPMRDEPGGSTIGVESVDVLGEHRHLREIGGIDVDEE
                     PMRTPGAPLMFGLRDPDGNNIYVVESPEVRD"
     gene            4564..7017
                     /locus_tag="PLJ64_06355"
     CDS             4564..7017
                     /locus_tag="PLJ64_06355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93135.1"
                     /translation="MRRPRHFRNRFFTSLAMAGLLALAAFSGTATAKVPAGSLDPSFG
                     GNRIPGISSVNFGDFDQASYWVPTAPLPDGSTYLMTDTPFRLFKVGSDGKLDQSFGNS
                     GVERIASTFGNKMIDWREVAAAPDGGAILIGAASAGSDVIGSDVIVVNRLLPDGSQNR
                     NFGPGHPVFFGKERWITGGVDKLGRILLVGYGGEAVMRLSPNGHLDGSFGTEGVARMD
                     LNATDYPHDVLATNDGIYVTFNNRTFRLTPDGKLDKSFGGVGVVRYGGDRISAASDGV
                     MLQTWNGKVRRLSLDGTPDSGYGTSGNGVAWGDATSNSTGAVLPDGSVIFAGDRTPGL
                     ENQLYSLNRLDPEGIADPSFGGDGSVEADWLDGSGTPGLWPGAGGSAVAWGGQPGRID
                     FREVDQAGNLNPDFGIGGVGSIRTGYLPDSNLTDSAQRRDHTYLSLGTIAQGSRYGYG
                     IAVASFGRNGRLETKFGDRGRLVLTQPAARFDPKPKMTLLPSGGAMICAKDGRDSVVW
                     RISRYGHLVKSFGDDGRLTLDFSPRCQDISFDGTGAVISAMDIVEDRRGVDLTRVLPD
                     GQVDRSYGVDGVAQRPEMADLDFYWVYSSSLATDRRGRTLFIASSGDARYIVRYTKNG
                     FPDKSFGIGGKLHYGTDSVSQEKDGQKPMYLPGLTKTNGVVLGRDGSIYLTGLYSKKP
                     FVAKLNSRGFPVRRYGKGGVVILPQANTSQKPYYRKPFPARPYGIGVRGDGSVVVTGI
                     SRPACAENWGCSRPLLVKRVEPNGKVDRAFAKRAFLSLGNRPNAIGNGVYFDGRRTVI
                     AGSMKFASERDNFMLAGFR"
     gene            7088..7543
                     /locus_tag="PLJ64_06360"
     CDS             7088..7543
                     /locus_tag="PLJ64_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93136.1"
                     /translation="MDGVSEDFSGLPEYKTRSERNAERVDRRFGQRRNNPYRGHRTRI
                     ILVGGFAVALVLLALSLVVLFAPTPYLGTNYASIAKSVGAASAHDCRPGGSGWVCTKE
                     VDGSAARYEVKVDWAGCWDGHLIGKPAGSNGAQPEISGCVSLMDHLTAG"
     gene            complement(7530..8198)
                     /locus_tag="PLJ64_06365"
     CDS             complement(7530..8198)
                     /locus_tag="PLJ64_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93137.1"
                     /translation="MAFFAALAAVLLVPSAAEAQTWSGGGITLPGPTTNPNIGKILPL
                     ARREFSRNVVERRGNNVPRYRNGKGRIAPYSISDQWCAAFSTWIWNHAGFKSYLGTSI
                     LWPSYDGTMVGVQVRDLTNWATRNGYFSYRAKPGYLVAYGTTHIGIVEKIDRTTGRAV
                     GSIEGNIGNRVNRVTVPMDRVTGYISPVRLTPIQLDKSAAYADMAVPPAALERAEASG
                     FSQP"
     gene            complement(8328..8957)
                     /gene="pdxH"
                     /locus_tag="PLJ64_06370"
     CDS             complement(8328..8957)
                     /gene="pdxH"
                     /locus_tag="PLJ64_06370"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HNC93138.1"
                     /translation="MSFPSPDRQPLARTDLDPDPIKQFGAWFEAAAEIVALPEAMTLA
                     TVDEAGRPDARMVLLKGHGADGFRFFTNYEGVKAAQLDASGEAALVFFWAPQDRQVRV
                     RGPVRRLDADASDRYFASRDRASQVGAWASPQSQPIPDQREALEELAEAAADRFAEGE
                     VPRPQHWGGFILEPRSIEFWQGRGARLHDRFRYDRQDSGEGWKITRLAP"
     gene            complement(8997..10070)
                     /locus_tag="PLJ64_06375"
     CDS             complement(8997..10070)
                     /locus_tag="PLJ64_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93139.1"
                     /translation="MSILIAWLIPAGAKAATVTYPSGNVSTFATGNGGWTSSTDYGGV
                     CVPAVTCPELSGSYQSTGGAAGGSDGYIRTDSGELTVASLLSTSTHTWRSPAFTYNGV
                     GGAQPEGLHFSVGVNPAVAQLLNLGVDIGISARFTGVSGGGDQIVIDDVTPGTASGWK
                     TLNGQIGPDALEVGKRYRVELSVSVGGLAAVLPSGSIGFDDVSLRATGPSGNGNGNGN
                     GNGNGNGNGTGNGNGNGNGGSTVLPPPKVIPAGKAFLYRNKLYIRVKCPRAFRPRCRI
                     NLVALTRKRHGRKLTPVRRANVRRGHSGRKVLGVRRPFRVKVRKMAKAGKRIVVRQKI
                     RSKRGRKHAIRYQRLRVVIRTRK"
     gene            complement(10276..10797)
                     /locus_tag="PLJ64_06380"
     CDS             complement(10276..10797)
                     /locus_tag="PLJ64_06380"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HNC93140.1"
                     /translation="MESLKGRLLVAAPSLFDFFRRTVVLVVEHTDQGAFGVILNRRAD
                     TEVAELVPDLGPLVEQGAPLWVGGPVGPDSIVVLGDFNHPDQSAGVVEGSIGVVDPDG
                     DADFSRARVFVGHAGWGPGQLEGEVERDSWIVSDFDPDDVFSEGDLWAEVVRRPGGEL
                     AILATMPDDPTLN"
     gene            10904..>11517
                     /gene="rpsD"
                     /locus_tag="PLJ64_06385"
     CDS             10904..>11517
                     /gene="rpsD"
                     /locus_tag="PLJ64_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNC93141.1"
                     /translation="MGRYTGPKEKLSRREGVDLGLKGERALGGKSAMERRPYPPGQHG
                     RGRRRASEFSVRMRAKQRAKAMYGLREKQFAKLFDRANREEGMAGENLMRHLESRLDN
                     VVYRLGLASTRAQARQFVVHQHIQVNGKRLDRPSYEVSAGDEIAVKPGTAIEAMARQS
                     TESAGPTPAWLLADHDGLKGTVNRLPDRSDIAAPIEERLIVEHYS"
BASE COUNT         2045 a         3776 c         3698 g         1998 t
ORIGIN      
        1 aggcagcccg gccacgaatg tcatgagcaa cctcacatcc gttgggtcgg acgacctgat
       61 gacaggcgag ggctggcgca cgatcgagca cgagtccgtt tttcgcgtca ccgttgtggt
      121 tgtgaccgga actgtcgata agggtcgagg acccgagcgg atcatcgagc cggtaccagg
      181 cgatcgggtc atcctgggcg accagatctc tgtacctcgg cggcacgact ctgtaagcgc
      241 cgtgagtgct cgcggtatga ccaaccttgt cctctgccga cgcagcacgg gaaaagttac
      301 cgagctgtga ggtactgacc gggtccacta cgcagctacg aatgcccgaa gcccccgcgg
      361 gcgatccagc gagcgggtcg gagcaggttc ccgaggctgg cgggttttca cccagaacat
      421 aggccggtac gacgttctgg tttgcgtctt gggagatcgc aggcaggtgg atctcggggt
      481 cggtcaggtc aacaagccag tcaaagctcg cagggtcccc gatgttgccc agccggtcga
      541 ttgcccgaac ccggaaattc cggatgccct cgggagccac gtaggtgatt tcgcctattg
      601 aagcgaaagc atcgtcgttg ccgcaagtga accaggcccc gccgtccttg ctgcactgga
      661 agctggcaac gccggaacca gtctcgctga aggctgcgtt gaagtcgtta gcgctaaagg
      721 tgaagttcgc gttggtcgag gcggtcgggt tgggcaccac gctgttgatc gatgtctgag
      781 gacccgtccg gtccacatag atcaccgcgt ccgccccgat tgaagccata ccaccggtct
      841 tgtagggaag tagcggatcc tgaatcatgt cgaggcggta tttgccttcg ggcagaatgt
      901 gggtggtccc gaggttgtgc atggtcagcg accagtcgcc ggtgaacgcc tcgacattgc
      961 ccgtgccgtt cgagacgatc ggatctccaa agctgtaggg caatttgtag gcgtacacgt
     1021 tcacggtggg ctcgtaaacg agaaaattgg cactggcatc gccgctcagg ccccacccgt
     1081 cgttgcctgt gatgtaaggg aaagcggaat tgacgtttat cgacgctgcc tgagcgccgc
     1141 tggcaccaaa agccaaggcc gccgacatgg ccagaaagat ggccgccagc ctcacaattt
     1201 gcctagcccc cgagatcatc tcgcggaagc atagcgaaca gtgtccggtt acgccagttc
     1261 ggcggtgatc acgtaccgga gaacggccgc gacctgctcc ggggtgcggg cggtggcgcg
     1321 ggcggcggcg tccacttcct tgagggcgtg atcgagttcc tcgtcgtgct gggtgattac
     1381 gggctttccc agcgctgaag cgaatccggc gtcgaaggca gcgttccact ggcggtactt
     1441 gtcgccgaag cggacaacga cgacgtcggc ctcctgaatg aaggtctggg tcctgatcga
     1501 gttcaccccg gcacccttgt gatcggccca gaacttcttc tcctcgtctc cgagggtcga
     1561 ggttccggcc gcatccgaag cagggtgatc ggtcaccggg gcggtgaaaa cgaccggtag
     1621 cccctcgcaa gcggcctcga cccgatcccg ccagtcggag tgaatttcgc cggcaaggta
     1681 gacgcgccac gttcgttcag acatgtgccc aacctacccg tcaagggaaa gaaaatggac
     1741 cgtgcaaccg ccttcgaaac gaagtcatcg ggcgtgtccg tcgagttcgg ctcgggttag
     1801 gctcacccgc ggcacccgta gtttccgctt aaggctgctt ccttccggac ctgacctgat
     1861 tcacgggctt gcgtcgcgcg ggacctgacc gtcaacacct ccctcggtgt actgaccccg
     1921 atggccagac cctcagaacg gcattcggcc ttgctaaagc ggattgcaag tgacagggca
     1981 ccgctgactc cccgcctagc acggtccggt tggaatccta gcgccccgcg cagggttcag
     2041 cagtcagcga gggcgtcggc gtagaaagcg aggtactcgt cccagtgggc gttccagtaa
     2101 tcctcatcgt ggccgccggg ccagttcagc ttcacggtgg cgtcgacccc ggccgcttcg
     2161 agcgagttcg ccagttcggt gacgccgggt acgaaggggt cgctcttgcc ggcgtcgagg
     2221 aagaggggtt gcgacgagaa agcatccggg tcggctgcgg cggtagcgag cacgtcgttg
     2281 tcgtcgaagt cctcggcgtc atcgaaggcg ccgggagccg actccccgcc gctcagaaat
     2341 agggccggcg agtgaccgcc gacagcgcag aacatccccg ggttctggat cgccaggtcg
     2401 agcgcaccga aaccgcccat cgagattcca ccgatcgcaa cccggtccgg gtcggccccg
     2461 aacttccctg ccatctgcgg gatcacctcg tcagtgacgt agctgcccca gtttccggtg
     2521 tcccggtcat gccagtagct ggactcgcct ccgtcgggaa agaccacgat cggtgcgtcc
     2581 ccgccctggc ctgccagcgc ctcgaagaac tcctcgtcat ccgtgtagct cccgtcgtca
     2641 ccgctgcgac cgtggaggaa cacgagcatc ccccgctcgc cgtcatcggc accatccggc
     2701 acgaccaccg aaaccgggtg ttcgccaggt accgccttcg actcgatcga ggtctcggtc
     2761 agctcggccc cgtgggtgtc ggtcttccac aagcggctca cgttcaacgc cgcccacgcg
     2821 acgatgccca aggcgatcag gacgacaagc agtgcgaccg ttcggcgcac ggccagcgat
     2881 cgccttctgt ctctgcttct cacggccaag ggccgaaccc tacgaccgat tcaagggccg
     2941 agggtgtacc ccacgtggac cattgtcgct ggcggagtcc ccgaaacggc acttcggatg
     3001 acgagaagtg aaccggacgg aacgctcacc tcgttcgagt tggaacaaat tttcccgcct
     3061 ccgttgaccg aacaaccgag aatccccgtt accgttcccg cctgatcgac cgtgccgatg
     3121 gaaatcgtgc ggaagactga gccgtcgccg ccggttggag tgtcgaacag caccgagagg
     3181 ttgtctagtt tcacattgac gccaggagca cgcgtcgcca cctggagcgc cgacgatgag
     3241 ctcaccggag tcgataatcc aagcgggctg aagagctggg tggtcgatcc gaggcttgcg
     3301 actgcaccga gagcggcggt ggtgctgggg cggcggtcat gcgaactcct ctcccgtcgg
     3361 gggccggtcg ggcccgtggc tcctgccgtt ccggtcgcgc cggtggttcc cgcctcgcct
     3421 gccgatccgg tcggaccggc ggcgccggtc aaccctgtcg taccagctgg accggtggca
     3481 ccagtcgatc cagtgacccc ggccgcgccg gtcgcgcccc ttggcagctg tcccttcttg
     3541 aaatcggccg cgagcaagga acggttcctg accttggccg agttgaccgc gttcttcttc
     3601 agttgcttcg tcccgacgct gttcttcggg atctgaacgg ccgcgtacga aacgccgccc
     3661 agggcgatga acaacgcgac cagggcgacg acattcgcga agcccggctt cccccagagt
     3721 tccttgagtc tcccccgcat tgggaagaac ctatccggct ggcccgggta tttccacctc
     3781 tgagccgcta cccgtctctc aggccgtcca actacaatgc ccgttcccgg gcgcgtagct
     3841 cagcgggaga gcgctcgctt cacacgcgag aggtcgctgg ttcgaaacca gccgtgccca
     3901 tccggtcagc aagcccgacg ggtcgtttgg aagcaacggg accgcggatt tccccgaccc
     3961 cgagcggggc gcctgattcg ccggtacgga tccgggcata ggatcgataa gaccacgacc
     4021 gaccggagga tccatgtcag attcagccac cacagtcaac aacctcggag tcgcgatgtt
     4081 caccgttgcg agccaggatg acgcgattga cttctacacc gggaagctcg gttgggaact
     4141 caggggcgac caggccttcg gcgaggacgg cagcatgcgc tgggtcgagg tcgcaccacc
     4201 cggctccgga gcccggctcg ccctgaaccc cccgatgcgg gatgaacccg gcggcagcac
     4261 gatcggggtc gagtcggttg acgtgctcgg cgagcatcgc cacctgaggg aaatcggcgg
     4321 aatcgacgtg gacgaggaac cgatgcggac ccccggcgcc ccgttgatgt tcggcctgcg
     4381 tgaccccgac ggaaacaaca tctacgtggt cgaatcaccc gaggttcgcg actgaggttc
     4441 cggacgaaag tccggtagaa agccgcaacc gtccgggcgg tactttcagt tcagggatgg
     4501 gttttaggga tgcctgccga tttcggcagc ccgtccagtc cagcccgcca caaggaaatg
     4561 cgtatgagac gcccccgaca cttcaggaac cgtttcttca ccagcctcgc gatggccggc
     4621 ctgctggccc tggccgcatt ttccgggacc gccacggcca aagtccccgc cgggtcgctc
     4681 gacccgtcct ttggcggcaa ccggatcccg ggaatcagca gcgtcaactt cggtgacttc
     4741 gaccaggcca gctactgggt gccgaccgcc cccttgccgg atggttccac ctatctgatg
     4801 actgacacgc cttttcggct tttcaaggtc gggtccgacg ggaagcttga tcagtcattc
     4861 ggcaattccg gcgtggagcg gatcgcttcg acctttggta acaagatgat cgactggcgg
     4921 gaagtcgccg ccgcaccgga cgggggagcg atcctgatcg gtgccgcgag cgccggcagc
     4981 gacgtgatcg gcagcgacgt aatcgttgtc aaccgcctgc ttcccgacgg aagccagaac
     5041 aggaatttcg gcccggggca tcccgtcttt ttcggcaagg aacggtggat caccggtggc
     5101 gtggacaaac ttggccggat cctgctcgtc ggctacggcg gtgaagccgt gatgagactg
     5161 tcgccgaacg gccaccttga cggaagcttc gggaccgaag gggtggcacg catggacctg
     5221 aacgccaccg actaccccca tgacgtcctg gccaccaatg acggcatcta cgtcacgttc
     5281 aacaaccgga ctttccggct cacgccggac ggcaagctcg acaagagctt cggcggggtt
     5341 ggagtggtgc gttacggggg cgaccggatt tccgctgcct cggacggagt gatgctccag
     5401 acctggaacg gcaaggtccg gcgactttcc ctcgacggca cgcccgactc cggatacgga
     5461 acttcgggca acggggtcgc gtggggcgat gccacaagca actcgaccgg cgccgtactc
     5521 cccgacgggt cggtcatctt cgccggcgac cggaccccgg gattggagaa ccagctctac
     5581 tcgctgaacc gactcgatcc ggaaggaatc gccgacccgt ctttcggcgg ggacggctcg
     5641 gtcgaagccg actggctcga cggctccgga acccccggac tctggcccgg cgccggcggg
     5701 agcgcagtcg catggggcgg acaacccggc cggatcgact tccgtgaggt cgaccaggcg
     5761 ggcaacctga accctgactt cggcatcggc ggtgtcggct cgatcaggac cggctacctg
     5821 ccggacagca acctgaccga ctccgcccag cgccgggatc acacctattt gagcctcgga
     5881 accatcgccc agggttcgag atacggctac ggcatcgcgg tcgcctcgtt cgggcgaaac
     5941 ggcaggctgg aaacgaagtt cggtgaccgc ggacgcctgg tgctgaccca acctgcggcc
     6001 cgcttcgatc cgaaaccgaa gatgaccctg ctgccttcgg gcggggcgat gatttgcgcc
     6061 aaggacggcc gggactcggt ggtctggcgg atcagccgtt acgggcatct cgtcaagagc
     6121 tttggtgacg acgggcgcct gaccctggac ttctcgcctc gctgccagga catttcgttt
     6181 gacgggaccg gtgccgtgat ctcggcgatg gacatcgtcg aagaccgccg cggcgttgat
     6241 ctgaccaggg tgttgcctga cggtcaagtc gaccgcagct acggggtaga cggggtcgcc
     6301 cagcgaccgg agatggccga cctcgatttc tactgggtct attcatccag cctcgcaacc
     6361 gaccgcaggg gacggaccct gttcatcgcc tccagcggtg atgcccggta catcgtgcgc
     6421 tacacaaaga acggcttccc cgacaagagc ttcggcatcg gcggcaagct tcactacggc
     6481 accgattctg tatcccagga aaaggacggt cagaagccga tgtatctccc cgggttgacc
     6541 aagaccaacg gcgtggtcct cggccgcgac ggcagcatct acctcaccgg cctctattcg
     6601 aagaagccct tcgtggccaa actcaactca cggggttttc cggtgcgcag gtacggcaag
     6661 ggtggcgtgg tcatcctgcc ccaggcgaac accagccaga agccgtacta ccggaagccc
     6721 ttcccggccc gtccgtatgg catcggggtt cgtggcgacg gaagcgtggt cgtgaccggg
     6781 atcagccgcc cggcctgcgc cgagaactgg ggctgctccc gcccgttgct cgtcaagagg
     6841 gtcgagccga acggaaaggt cgaccgggcc ttcgccaagc gggctttcct gagcctcggc
     6901 aaccggccca acgcgatcgg caacggcgtc tacttcgacg gcaggagaac ggtcatcgcc
     6961 gggtcgatga agttcgcctc ggaaagagac aacttcatgc tggccggctt ccgctgacgg
     7021 cagctttccg ttcattagca gggattttcg gacctggctg aaagtggcgg tctactctgc
     7081 tcgcgcgatg gatggtgtga gcgaagattt cagcgggctg cccgaataca agacccggag
     7141 cgagcggaat gccgaacgcg tcgatcgccg gttcgggcag cgccggaaca acccttatcg
     7201 cggtcaccgg accaggatca tcctggtcgg tggtttcgcg gtcgccctgg tcctgctggc
     7261 cctctcgctg gtcgtcctct tcgcgccgac tccgtacctc ggcaccaact acgcctcgat
     7321 cgccaagtcg gtcggggccg catccgccca cgactgcagg cccggcggaa gcggctgggt
     7381 ctgcaccaaa gaggtcgacg gcagcgcggc ccgttacgag gtaaaggtcg actgggcggg
     7441 ttgctgggat ggccacctga tcggcaagcc cgccggcagc aacggcgcgc aaccggagat
     7501 ctccggctgc gtgtcattga tggatcacct tacggctggc tgaacccgga agcttcggcc
     7561 cgctcgagcg cagccggcgg gaccgccatg tcggcgtagg cggcactctt gtcgagctgg
     7621 atcggggtca gccgcaccgg gctgatgtat ccggtgaccc ggtccatcgg cacggtcacc
     7681 cggtttaccc ggttgccgat gttgccctcg atcgagccga ccgcgcgacc ggtggtccgg
     7741 tcgatcttct cgacgatgcc gatgtgggtg gttccgtaag cgacgaggta gcccggtttc
     7801 gcccggtagg agaagtagcc gttcctggtc gcccagttgg tcaggtcccg gacctggacc
     7861 ccgaccatgg tgccgtcata tgacggccaa aggatgctcg tccctaggta ggacttgaag
     7921 ccggcgtggt tccagatcca ggtcgagaag gcggcgcacc actgatccga gatgctgtac
     7981 ggcgcaatcc tgcccttgcc gtttcggtag cggggaacgt tgttgccgcg gcgttcgacc
     8041 acgttgcggg aaaactcacg ccgggccagc ggcaggatct tgccgatgtt cgggttggtg
     8101 gtcggaccgg gcagcgtgat gcctccgccg gaccaggtct gggcttcagc cgccgatgga
     8161 accagaagga ctgcggcgag ggcggcgaaa aacgccaacc ggacttttaa cggaaaaacg
     8221 gacatcaccc ctgaggcttg caggctaaac aggttcgggg caggttgtcc agccccctgc
     8281 gagatgtcat gcagccggtg accgcgaaag aaccggaccc ttttcgctca gggagccagg
     8341 cgggtgatct tccagccctc tcccgaatcc tggcggtcgt agcgaaaacg gtcgtggagc
     8401 cgggccccgc gaccctgcca gaactcgatc gagcggggct ccaggatgaa acctccccaa
     8461 tgctggggtc gcggtacttc gccttccgcg aaacgatcgg ccgccgcttc ggccagctct
     8521 tcgagcgcct ccctctggtc gggaatcggc tggctctggg gggaagccca ggcgccgacc
     8581 tggctcgccc ggtcccgact cgcgaaatac cggtcagagg catccgcgtc cagccgtcgc
     8641 accgggcccc gcacccggac ctgccggtcc tgcggcgccc agaagaagac cagggcggcc
     8701 tccccgctgg catcgagctg ggctgccttg accccctcgt agttggtgaa gaaccggaat
     8761 ccgtcggctc cgtggccctt gagcagaacc atgcgtgcgt caggtcgccc cgcctcgtca
     8821 accgtggcca gggtcatggc ctccggcagg gccacgatct cagcggcagc ttcgaaccac
     8881 gctccgaact gcttgatcgg atcgggatcg aggtcggtgc gggcgagggg ctgacggtcc
     8941 ggcgagggaa aagacacggg ccaagcctac cggggcccgg cccgtgccat ttgcctctac
     9001 ttgcgggtgc ggatcaccac gcgaaggcgc tggtatcgaa tcgcgtgctt gcggcctcgt
     9061 ttggacctga tcttctgtcg cacgacgatc cgtttcccgg ccttggccat cttccggacc
     9121 ttgacccgga acgggcgcct gacgccgagg accttgcgtc cggaatggcc ccggcggacg
     9181 ttcgcccggc ggaccggggt cagctttcgt ccgtgccgct tcctggtcag ggcgaccagg
     9241 ttgatccggc agcgcggccg gaaagcccgc gggcacttga cccggatgta gagcttgttc
     9301 cggtagagga aggctttccc ggccggaatc accttcggcg ggggcagcac ggtgctgcca
     9361 ccgtttccgt tgccgttgcc attgccggtg ccgttgccgt tcccgtttcc attcccgttg
     9421 ccgttgccat ttccgttgcc ggagggaccg gtggctcgca gcgagacatc gtcgaacccg
     9481 atcgagccag acggcaggac cgcagccagg ccgccgaccg aaaccgacaa ttcgacccgg
     9541 tatcgcttgc cgacctcgag cgcgtcgggc ccgatctggc cgttcagcgt cttccagccg
     9601 gaggcggtgc cgggggtgac atcgtcaatc acgatctgat ctccaccgcc ggaaacgccg
     9661 gtgaacctgg cgctgatgcc gatgtcgaca cccaggttca gcaactgcgc caccgcggga
     9721 ttgaccccga ccgagaagtg cagcccttcg ggctgtgcgc cgccgacccc gttataggtg
     9781 aaggcggggc tccgccaggt gtgggtggag gttgaaagca gggaggcgac ggtaagttcg
     9841 ccggaatcgg tccggatgta cccgtcgctt ccgccggctg cgccaccggt tgactggtag
     9901 ctgccggaaa gctcgggaca ggtcactgcc ggcacacata ctccgccgta gtcggtgctc
     9961 gaggtccagc caccgttgcc ggtggcgaaa gtgctcacgt tcccgctcgg atatgtcacg
    10021 gtggccgcct tcgccccggc cgggatcagc caggcgatca gtatcgacaa cgccgcgatt
    10081 gccgcagcag gcaagagtct gctccgcata atgctcatct cttctctcca ttgagttcgg
    10141 gttggcgatc cgtgccgttc ggcccagggg gatcgcttac cgttcaatcg ggtgacggcg
    10201 ttaccgccac cgccaacgga accactattc ccgaagccac cgcttcgcaa acagggtgtt
    10261 ccggtcctct tgctttcagt tcagagtcgg gtcatccggc atcgtggcga ggatcgccag
    10321 ttcgcccccc ggccggcgaa cgacctcggc ccagagatcg ccttccgaga agacatcatc
    10381 gggatcgaag tcggaaacga tccaggaatc gcgctcgact tcaccttcga gctgtcccgg
    10441 cccccagccg gcgtgtccga cgaagacacg ggcccgggag aagtcggcat caccgtccgg
    10501 gtcaaccacc ccgatgctgc cctcgaccac cccggccgac tggtccggat ggttgaaatc
    10561 cccgagcacg acgatcgagt cgggaccgac cgggccaccg acccagagcg gagccccctg
    10621 ctcgaccagc ggcccgagat cgggaaccag ttcggccacc tcggtgtcgg cgcggcggtt
    10681 gagaatcacc ccgaaggccc cttgatcggt gtgttcgacg accagcacaa cggtgcgccg
    10741 aaagaaatcg aaaaggctcg gtgcggctac cagcaagcgt cccttgagcg actccatgta
    10801 ccgactatag gacgctgttc ggcgtcgagt ggacacggac cggccatggc gctgccgcac
    10861 aaagcgggaa ttcgcccgat ccggtaggct ccttcgccgc actatgggca gatacacagg
    10921 accaaaagaa aagctttcgc gccgtgaggg cgtagatctc ggactcaagg gtgaacgggc
    10981 cctcggcggc aagagcgcca tggagcgtcg cccctacccg cccggccagc atggccgcgg
    11041 ccggcgccgt gcttcggagt tctcggtccg tatgcgtgcc aagcagcgcg ccaaggcgat
    11101 gtacggcctt cgcgagaagc agttcgccaa gctcttcgac cgcgccaacc gtgaagaagg
    11161 catggccggc gagaacctga tgcgtcacct cgagagccgt cttgacaacg tcgtttaccg
    11221 gctcggcctc gcttcgaccc gcgcccaggc ccgccagttc gtcgtccacc agcacatcca
    11281 ggtcaacggc aagcgcctcg accgtccctc gtatgaggtt tcggccggtg acgagattgc
    11341 cgtcaagccc ggtaccgcga tcgaggccat ggcccgccag tccaccgagt cggctggccc
    11401 gaccccggcc tggctcctgg ccgaccacga cggtctgaag ggcacggtca accgcctgcc
    11461 cgaccgcagc gacatcgccg ctccgatcga agagcgcctg atcgtcgagc actactc
//
LOCUS       DAOQKN010000071        11433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_2655_length_11433_cov_5.836351, whole genome shotgun sequence.
ACCESSION   DAOQKN010000071 DAOQKN010000000
VERSION     DAOQKN010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 11433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11433
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2655_length_11433_cov_5.836351"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(421..876)
                     /locus_tag="PLJ64_06390"
     CDS             complement(421..876)
                     /locus_tag="PLJ64_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93142.1"
                     /translation="MSLCFVDPADAIHVISESVGREARRQILGEALRAWVDEIPEEEL
                     EAQYARALADLDQDDLSLLAAWHGSMDLGQPVPNRDFLMGAFGSLGEHRREALKLAAG
                     FRGEEVLEDGLTEELALETVVGWLSPERLLDLANEAVELYVWDRIGSDN"
     gene            1050..4004
                     /locus_tag="PLJ64_06395"
     CDS             1050..4004
                     /locus_tag="PLJ64_06395"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNC93143.1"
                     /translation="MPLTIIKGPPNSGRTELVRERFESRIADDPVLVVPSTDDIFGWE
                     RRLTRGSGAFLGGTVVHFKDLIELILGDGSAARSYEKGSDVACPLRRRALATDAIHAG
                     WPAIAGRLEDQPGLVDSALQVIDEFRAALISPKEFDDPAISDLAGPAAGVFRLYLDGL
                     HEAGLTDLPERSVRATTHSLDSWEGRPVFVAGFDDLTRQQLDLLRRLSGKTDVTIAIT
                     HEQGNEAMAITESLLGRLREIGGTVERETERPAEQADHEGLLVELERSFLDPGKAGTI
                     KPGGALRLIEASGRRGEAEAIGAEIARLVSNGTDPGEIAIAIDAPATNGTALLEVLTE
                     YEIPAMLEAETTAPESAIGQSAINFLLATARNGPADRLFAWLRGPLGLDPDLIDQLEF
                     RAVRSGTESAREVIAAMNPAQRRGLPGLAEGENGDPSEAVRSVVRHGARVLLAERQDS
                     IPASSIATETQMASAISRACDELEGIHGDHLSARILIDALASGSVKTWAVPTAHTVRI
                     GSPYAMRAKRVEYLFIASLQEKDLGGDQSSPLISNEARSALGMPELADQEEQETYLFY
                     SCLAVPTRGLWLSHRVADVHGKSEFPSAMIGEVTRLFEGEGAGLDLIRRSSSDIIFPV
                     ADSPSRHEWAISIAAAGEAVDPLGSDAAKEVVLEVGRSREVEASTSTLGDIDSPAAAA
                     ALAERNVFSATALEAFIECPYRWFFERAMNPVRFGPEPEPLAKGNLVHEVLAALYSSR
                     RATIPNGDDIEDWIAAARTLVDELSGQCDLGGDSARHRIQRRQVSIEIERFLRREAER
                     ETAFRPVDLERMFGFEREDSLPPLEFDGWKLRGKIDRIDQDATGRGIVFDYKSGSSSY
                     KSRAELKREGKVQLPLYLRALEVLWDLDPAAGLYVPIFAAKHQPRGLVSEAASPDLKD
                     LNPVSKDFVEDLPEEIQQDVEQAAEAAWKILHGQIAHDPTECINHFSHAGVPDWNPDV
                     EENFTGATFRWP"
     gene            3995..7582
                     /locus_tag="PLJ64_06400"
     CDS             3995..7582
                     /locus_tag="PLJ64_06400"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNC93144.1"
                     /translation="MALTSEQKTAIDCDDRDILLQAGAGSGKTSTTVNRYERLLTGVR
                     TDGRTSEVLEPSAILVFTFTDKAAGELRERIRRLRESGGREFSMGSIWVGTFHSICAR
                     ILRAHPVAAGVDPAFDVIDDVVAARLKESAYEAGLEQTCEVEGAIELLSRFNQNTLKW
                     GIQAAYERLRAAGEFDPRLPDPPEPRPLETIQLEVLKVALRARGDSSLTASYKTKIGN
                     LIDYLEATDPPDFCFDGLDGVDFYKTRSKKIPELCDAFREAKTELLTRELGPQYWDLL
                     SNLLSNYGTAYDRAKDRRGGLDFEDLQLKTLDLLLRREVIADSYREQFAEIMVDEFQD
                     TNRLQMDLIDKLRGEETTLFTVGDEMQAIYGFRYADVELYRQRRAGISFRLPADAERR
                     AAGERDRVTFEVTTLPLSANFRSQAPVIGAVNELGNQFESLIEGIRTDDEPGGETGGT
                     RHEFEHLRVGWSPPEPGSGPAPPPEPKVEILLTKAKAWQQVDLGPLSPAPTGDQYMTE
                     SRGQHEAEALALARHIRKAVDDGIPPGDIVVLLRTKTRMWLFEAALKQAGLRPYVVGG
                     TGFWETREGIDMRALLATVANPLDDEALAGALAGPACGLSPSALWLLGSRRDHDQPLW
                     PLVSNLADKPDPEISEEDNRRAGEFVATIRRLRAKLAGLPLSAVVQTAVSETGYDLVN
                     LMRDPSGSGLANIRRVAEIASEFEATEGRDLRGLIEWIDDSARLDSEQAVATEDEESD
                     VVRLMTIHKSKGLEFPMVCVADLGLASKADSENVIWISPRNDDPGKFEVGLRLPEPGG
                     PGLDLFAWQTLKDQARTDKTDEELRILHVAMTRAESRLVLSGVTDFEKIPDPTEANPF
                     ATRLTQAYGVEDFPGTIPVPAPDFLVAPAETPAISIEVILNRPDDAGDLAEVTSPESL
                     NRADASTGEPPIGRPVSPGFPEVPLSYSALASYRECPARFFATRVLKLDEPERRHGDA
                     KQPLDPEMEIRKEIDATSFGVAVHELLELLPARRWIRPADAEIDSRLEANGVPGADAG
                     DLAREMLDGFLASELAGEIKGRRAEVETGLLVEVGGVMVRGFADLLVRDPVKPLILDY
                     KTNQLEETSIQELMSKYDLQRDLYALAVSKAAGVEELETAFVFLRAPDQPVRDHYDSK
                     RLSEAENSIAGLIDDIVAGRYLGGPDAGRQPCGDCWACETLGVQKGTSG"
     gene            complement(7561..7893)
                     /gene="xseB"
                     /locus_tag="PLJ64_06405"
     CDS             complement(7561..7893)
                     /gene="xseB"
                     /locus_tag="PLJ64_06405"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNC93145.1"
                     /translation="MSEETPEAPEHSYESATARLDEIIQRLDSGDAQLRETLELCEEA
                     KGLIEYCAGELAAVDQGLKELKLEDLASSLTGPGNGGSEPPVPEPAGPPEPEHEVPPP
                     PDQPDVPF"
     gene            complement(7886..9343)
                     /gene="xseA"
                     /locus_tag="PLJ64_06410"
     CDS             complement(7886..9343)
                     /gene="xseA"
                     /locus_tag="PLJ64_06410"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNC93146.1"
                     /translation="MSEELPEPGNPAAAEDAAPTPVGDYARQLKQDLKRLPEATILGE
                     ITNFSQSRVQIYFELRDERGGVPGTMWKREFDALGLPAGTVKVGSQVVVTGAPDYYEG
                     GKNASPSFSFRAIDIRPFGEGDLIARLAALRKQFQAEGLTEPQKNLPRRLLPRRIGVI
                     TAEGGAARQDLMVGLERRGWKGEVVWGFAPVQDRKAAPQITRILSDMAAFADVDSIVV
                     CRGGGSLTDLWAFCDEDLCRTVVMLAVPVISAVGHERDVTLLDDVAAVRASTPTHAAE
                     AVVGIDIPAARASLARAAGTTRRVSQAAVRDRIAPLAAMAAGPGRAIRNERGKLNQKT
                     REIRAAADRGIQGRRGSAQRVLDQCLLPAASRTNRLIEAAGTRNGARPGALQARVLGR
                     LGFAGRKLETERVALRAHDPQRTLERGYARVEDRKGDPVVDSSGARRAGQVRIRFADD
                     AVDAVVGDTRRRRSRQNEVEQEFEQIRMEGLDEGE"
     gene            9564..10586
                     /locus_tag="PLJ64_06415"
     CDS             9564..10586
                     /locus_tag="PLJ64_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0043199 - sulfate binding [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNC93147.1"
                     /translation="MSFSSSKKTRRSIQALLVLALVAFGAIIAGCGSSDGDSGSKVQL
                     VAYSTPQTAYEEGLEPAFNETPEGDGVEFSNSFGASGDQSRAVEAGQPAGYVNFALET
                     DVTRLVDAGLVSKDWQNNKYKGIVTNSVVSIVTRPGNPEGIESFQDIIDKDVSVVTPN
                     PFTSGGARWNIMAIYGAALHEGKSEQEALDTVKQVLSQAEVQPASARDALQAFVGGAG
                     DVLISYENEAIAAQNAGEDVDYVIPDSTILIEQPAAVTVDAPKSATDFLNYLWSDAGQ
                     KIWAEQGYRPVNKSLVDESVYPTPKDEFTIAEFGGWDKVADEFFDDKTGSIAEIEKEL
                     GVSTEG"
     gene            10651..11415
                     /gene="cysT"
                     /locus_tag="PLJ64_06420"
     CDS             10651..11415
                     /gene="cysT"
                     /locus_tag="PLJ64_06420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HNC93148.1"
                     /translation="MTKGLVVTYLSLIVLIPLAAIAAKAFSAGPAEFWDAIRQPQAIA
                     ALKLTAICSLIVVAINAVMGTLIAWVLVRDEFPGKSIVNSMIDLPFALPTIVASLTLL
                     TLYGNDSFLGIHAAYTKFAVIIALLFVTLPFVVRAVQPLLLSLDRDMESAAESLGAGK
                     VRIFTQIIFPNLLPGIAAGVALAFARALGEFGALVLISGNVPYKTEVASVFIRGQIES
                     DQVVGAAAVSVVLLVFSMCMLAFVNLIQKWGLRHDR"
BASE COUNT         2042 a         3764 c         3696 g         1931 t
ORIGIN      
        1 actggcacct ccggaactga ttcgcaagtc tcgtgggtcg gattttcggc ccgactgcgg
       61 agaatccgac ccacctgggc ggtgcagggc aggtgaaggg gaagttgaag gaacccaggg
      121 tccgggctgg ctccggggcc gaattcgcgt cctggttcag ggcggcaccg tcccccgagt
      181 cacggtcggg aatcgatcga gatgctgttt gtcggcgata tatgccgaca aacagcatct
      241 cgcgaaatgc caacaccacc aaccctgagc gggtcgtgca caggggcctc cagccgttcg
      301 ctggcaagga gacaggccgg aaatgaccgc gatagtacgc gggtacgtga gctgtcgttt
      361 ccggccgccg acgaagtcag cgtgcggctg gtgggtcctg ggcgcgttgc ccgggggctt
      421 ctagttgtcg gagccgattc gatcccagac gtacaactcg accgcctcgt tggcgaggtc
      481 gaggagacgt tccggggaga gccagccgac caccgtttcg agggcgagct cctcggtcag
      541 gccgtcttcg aggacttctt ccccgcggaa gccggccgcc agcttcaggg cctcccggcg
      601 gtgctcgccg agcgaaccga aggcgcccat caggaagtcg cggttgggaa ccggctggcc
      661 caggtccatc gagccgtgcc aggcggcgag cagggagagg tcatcctggt cgaggtcggc
      721 gagggcacgg gcgtactggg cctcgagttc ctcttcgggg atctcgtcca cccatgcacg
      781 cagagcctcc ccgaggatct ggcgacgggc ctcgcggcca accgattccg aaatgacgtg
      841 aatcgcatca gccggatcga cgaaacagag tgacatgttg ggccgcctcg gggttgctta
      901 ctcggacaac cgtgaagata gagatgccac aggacgcaaa aaatgttgca gaacctcgca
      961 atttgcggga gggcaagcgg aaaacgaacc tttccgcaaa gccggcgatg tcagaaaacg
     1021 cccgccgctc ccgaccataa agttgccccg tgcccctgac tatcatcaaa ggccccccga
     1081 actccggccg gaccgaactc gtccgcgagc gcttcgagtc ccggatcgcc gacgatcccg
     1141 tcctcgtcgt gccctccacc gacgacatct tcggctggga gcgacgcctg acccgcggaa
     1201 gcggagcctt cctcggggga acggtggtcc acttcaagga cctgatcgaa ctcatcctcg
     1261 gagatggatc ggcggcacgc agctatgaga agggcagcga cgtcgcctgc ccccttcgtc
     1321 gtcgcgcgct cgctaccgac gccatccacg cgggctggcc tgcgatcgcc ggccggctgg
     1381 aagatcagcc cgggctggtc gactcggcgc ttcaggtaat cgacgagttc cgggccgccc
     1441 tgatttcccc gaaggagttc gatgatcctg ccatttccga tctggccgga cctgctgcgg
     1501 gggtcttccg cctctacctg gacggactgc acgaggccgg actgaccgac ctgcccgaaa
     1561 ggtccgtgcg agccacgact cattcactcg actcctggga gggtcgtccc gtcttcgtcg
     1621 ccggcttcga tgacctgacc cggcagcagc tcgatctcct ccgccgcctc tccggaaaga
     1681 ccgacgtgac gatcgcgatc actcacgagc agggcaacga ggcgatggcg atcaccgaga
     1741 gcctgctggg acgactcagg gagatcgggg ggacggtgga acgggaaacg gaacgtcccg
     1801 cggagcaagc cgatcacgaa ggcctgctgg tcgagctcga gcgaagcttc ctcgaccccg
     1861 ggaaagccgg aacgatcaag cccggtggcg ccctcagact gatcgaggcc tcggggcgcc
     1921 gcggggaagc cgaagcgatc ggcgcggaga tcgcgcgcct ggtcagcaac gggaccgatc
     1981 ccggagagat cgcgatcgcc atcgacgctc cggcgaccaa cgggaccgcg cttctggagg
     2041 tgctcaccga gtacgagatc ccggcgatgc tcgaagccga aacgacggca ccggaatcgg
     2101 cgatcggcca gtccgcgatc aacttcctcc tggccaccgc ccggaacgga cccgccgacc
     2161 ggctgttcgc ctggctgaga ggcccgctcg gtctcgaccc tgacctgatt gaccaactcg
     2221 agttcagggc ggttcgctcg ggcaccgaga gcgcgcggga agtgatcgca gcgatgaacc
     2281 cggctcagcg ccgcgggctg cctggcctcg ccgagggcga gaacggagac ccgtccgagg
     2341 cggtcagatc ggttgtgcga cacggagcaa gggttttgct ggccgagcgg caggattcga
     2401 tccctgcctc ttcgatcgcc accgagaccc agatggcgag tgcgatcagc cgtgcctgcg
     2461 acgagctgga agggattcac ggcgaccatc tctcggcccg gatcctgatt gatgccctgg
     2521 ctagcgggtc ggtcaagacc tgggccgtgc cgaccgcaca cacggtgagg atcggcagcc
     2581 cgtacgcaat gcgggcaaaa agggtcgaat acctgttcat cgcttcgctt caggagaagg
     2641 atctcggtgg cgatcagagc agccccctca tctcgaacga agcccgctcg gcgctgggca
     2701 tgccggagct ggccgatcag gaagagcagg agacctatct cttctattcc tgcctcgcgg
     2761 tcccgactcg cggcctctgg ctctcccatc gggtggccga cgtccacggc aagtctgaat
     2821 ttccctcggc gatgatcggg gaggtgacgc gtctcttcga aggcgaaggg gccggcctcg
     2881 acctgatccg gcgatcgagc tcggacatca tctttcccgt cgccgattca cccagccggc
     2941 acgagtgggc gatctcgatc gccgccgccg gcgaagcagt ggacccgctc ggctcggacg
     3001 cggcgaagga agtcgtcctc gaggtcggac ggtcgcgtga agtcgaagca tccacctcga
     3061 ccctgggtga catcgattcc cctgcggctg ctgccgcgct cgccgaacgg aacgttttct
     3121 cggcgaccgc ccttgaggcg ttcatcgaat gtccctaccg atggttcttc gagcgggcga
     3181 tgaatccggt gcgattcgga ccggagcccg aacccctggc gaagggcaac ctcgtgcacg
     3241 aggttctggc cgctctctac tcctcgcgtc gggcaacgat cccgaatggc gacgacatcg
     3301 aggactggat cgccgcggcg cgaacgctcg tggacgaatt gtccgggcaa tgcgacctgg
     3361 gcggagattc ggcccgacac cggattcagc gccggcaggt ctcgatcgag atcgaacgat
     3421 tcctgcgccg cgaggcggaa cgggaaaccg ctttcaggcc ggtcgatctg gagcggatgt
     3481 tcggcttcga aagggaggat tcgctgccgc cgctcgagtt cgacggctgg aaactgaggg
     3541 ggaagatcga ccggatcgac caggacgcca ccggacgggg gatcgtcttc gactacaagt
     3601 caggctcgtc ttcctacaag tcgcgggccg aactgaaacg cgaaggcaag gtccagctcc
     3661 cgctctacct tcgggccctc gaagtgctct gggacctgga ccctgcggcc ggactctacg
     3721 tgccgatctt cgccgcgaag caccagccgc gaggactggt ttccgaagcg gcaagccccg
     3781 acctgaagga tctgaatccg gtttcgaagg acttcgtgga agatctgccg gaggagatcc
     3841 agcaggatgt cgaacaggct gccgaagccg cctggaagat tctccacggc cagatcgcgc
     3901 acgatccgac cgagtgcatc aatcacttct cgcatgcggg agttcccgac tggaaccccg
     3961 acgtcgaaga gaacttcacc ggagcgacct ttcgatggcc ctgacttcgg aacagaaaac
     4021 ggcgatcgac tgcgacgacc gggacatcct gctgcaggcc ggtgccggtt cgggcaagac
     4081 gagcacgacc gtaaatcgtt acgagcggct gctgaccgga gttcggaccg atggcaggac
     4141 ttccgaggtt ctggagccgt cggcgatcct ggtcttcacc ttcaccgaca aggccgcggg
     4201 ggagcttcgg gagcgcattc gccgcctcag ggaatcgggc ggccgtgaat tttcgatggg
     4261 ttcgatctgg gtcgggacct ttcactcgat ctgtgcccgc atcctccggg cacatccggt
     4321 cgccgccggg gtcgatccgg ctttcgacgt gatcgatgac gtggtcgccg caaggctcaa
     4381 ggagtccgca tacgaggcgg ggcttgagca gacttgcgag gtggaagggg cgatcgagct
     4441 gctctcccgc ttcaatcaga acaccctcaa gtgggggatt caggccgcct acgaacggct
     4501 gcgggcggcc ggggagttcg atcccaggct tccggacccg ccggaacccc gtccgctgga
     4561 gacgattcaa ctggaggtcc tgaaagtggc tctccgcgcg aggggcgatt cgagcctcac
     4621 cgcgtcatac aagacgaaaa tcgggaacct gatcgattac ctcgaagcga ccgacccgcc
     4681 tgacttctgt ttcgacgggc tcgacggggt ggatttctac aagacgaggt ccaagaagat
     4741 tcccgagctc tgtgatgcct tccgggaggc caagacggaa ctgctcaccc gcgagcttgg
     4801 cccgcagtac tgggatctcc tctcgaacct gctttccaac tacggcaccg cctacgaccg
     4861 ggccaaggac cggcgcgggg ggctcgattt cgaggaccta cagctcaaga ctctcgatct
     4921 gctccttagg cgcgaggtga tcgcggattc ctaccgcgag cagttcgccg agatcatggt
     4981 cgacgagttc caggacacga accgcctgca gatggatctg atcgacaagc tccgtggcga
     5041 agagacgacc ctgttcacgg tcggcgacga gatgcaggcg atttacggat tccgctatgc
     5101 cgacgtcgag ctctaccggc agcggcgtgc cggcatctcg ttccggcttc ccgccgatgc
     5161 cgaacgccgc gcggccggtg agcgggaccg ggtcactttc gaggtcacga ctctcccgct
     5221 gagcgcaaac ttccgctccc aggccccggt gatcggtgcg gtcaacgagc tcggcaacca
     5281 gttcgaatca ctaatcgagg gcatcaggac cgacgatgaa ccgggcggag agaccggtgg
     5341 cacccgccac gagttcgagc atctcagggt cggctggtca ccgccggagc cgggctccgg
     5401 gccggccccg ccccccgaac cgaaggtcga gatccttctg accaaagcca aggcctggca
     5461 gcaggttgat ctcggaccgc tctcaccggc gccgaccgga gaccagtaca tgaccgagag
     5521 ccgcgggcag cacgaagccg aagcgctggc cctggcccgt cacattcgca aggccgtaga
     5581 cgacgggata ccgcccggcg acatcgtcgt tctgctcagg accaagacga gaatgtggct
     5641 cttcgaagcc gcgctcaagc aggccggact gaggccctac gtggtcgggg gcacgggttt
     5701 ctgggagacc cgcgaaggca ttgacatgag ggcgttgctg gcgaccgtcg cgaacccgct
     5761 cgatgacgag gcgctggccg gcgctttggc cggtccggcc tgcgggctca gcccgagtgc
     5821 actctggttg ctcggcagcc gtcgcgatca cgatcagccg ctctggccgc tggtctcgaa
     5881 cctggcggac aagccggatc cggagatctc cgaggaagac aaccggcggg ccggcgagtt
     5941 cgtggcgacc atccggcgcc ttcgcgcgaa gctggcgggc ttgccgcttt cagccgtggt
     6001 ccagaccgcg gtttcggaaa ccggctacga cctcgtcaat ctgatgcggg atccgtctgg
     6061 ttccggactg gccaacatcc ggcgggtggc cgagatcgcc agcgagttcg aagcgacaga
     6121 gggcagggat ctgcgggggc tgatcgagtg gatcgacgac agcgcgcgac tggactcgga
     6181 gcaggcggtg gcaaccgaag acgaagagtc ggacgtggta cgcctgatga cgatccacaa
     6241 gtcgaagggt cttgagttcc cgatggtttg cgtcgccgac ctcggtctgg cctcgaaggc
     6301 cgattccgaa aacgtgatct ggatctcgcc acggaacgac gatccgggca agttcgaggt
     6361 cggactgcga ctgcccgaac cgggaggacc cgggcttgat ctgttcgcct ggcaaacgct
     6421 taaggatcaa gccaggacag acaagaccga cgaagaactg cggatcctcc acgtcgccat
     6481 gaccagagcc gagagccggc tcgtgttgag cggcgtcacc gacttcgaga agatccctga
     6541 cccgaccgaa gccaaccctt ttgcgacccg cctgacccag gcctacgggg tcgaagactt
     6601 cccgggcacc atccctgtgc cggccccgga tttcctggtc gcaccggctg aaacccccgc
     6661 gatctcgatc gaggtgattc tgaaccggcc ggacgatgcc ggggatctgg ccgaagtgac
     6721 cagtccggaa agcctgaacc gggcagatgc gtctaccggc gagccgccaa ttggccggcc
     6781 ggtatctccc ggttttccgg aagtcccgct ctcctactcc gcccttgcct cctaccggga
     6841 gtgtcccgcc cggttcttcg ccaccagggt gctgaagctg gacgaaccgg aacgccggca
     6901 cggcgacgcg aagcaaccgc tggatccgga aatggagatc cgcaaggaga tcgatgccac
     6961 ctcattcggg gtggcggtgc acgagctgct ggaactgctg cccgcacgcc gctggatcag
     7021 gccggccgat gccgaaatcg actcgagact ggaggcgaac ggggttccgg gagcagatgc
     7081 cggcgacctg gcccgggaga tgctcgacgg gtttcttgct tccgaactcg ccggggaaat
     7141 caagggcaga agggccgagg ttgaaaccgg tctgctggtc gaggtcgggg gcgtgatggt
     7201 ccgcggcttt gccgatctcc tcgtccgcga cccggtcaaa cccctgatcc tcgactacaa
     7261 gacaaaccag ctcgaggaga cctcgatcca ggagttgatg tcgaagtacg acctccagcg
     7321 ggacctttac gcgttggcag tgtcgaaggc cgccggtgtc gaagaactgg aaaccgcgtt
     7381 cgtctttctg cgagccccgg atcagccggt gcgcgaccac tacgactcaa agcgtctctc
     7441 ggaagccgag aattcgatcg ccggtctcat cgacgacatc gtggccggcc ggtacctggg
     7501 cggaccggac gcgggaagac aaccctgcgg cgattgctgg gcctgcgaaa ccctcggagt
     7561 tcagaagggg acgtcgggct gatccggggg tggcggaacc tcgtgctccg gctcaggcgg
     7621 cccggccggt tcggggaccg gtggctccga gccgccattt ccggggccgg tcagggagct
     7681 ggcgaggtcc tctagcttga gttccttgag gccctggtcg acggcggcga gttctccggc
     7741 gcagtactcg atcaggcctt tggcttcctc acagagttcg agggtttcgc ggagctgggc
     7801 gtcgcccgag tcgaggcgct ggatgatctc gtcgagccgc gcggtggccg actcgtagct
     7861 gtgctcgggc gcttcgggcg tttcttcact caccttcgtc cagtccttcc atcctgatct
     7921 gctcgaactc ctgctcgact tcattctggc gcgaccggcg gcgtctcgtg tcgccgacca
     7981 cggcatcgac cgcgtcgtcg gcgaaacgaa tcctgacctg gccggcccga cgggctccgc
     8041 tggaatcgac taccggatct cccttgcggt cctcgacccg ggcataaccc cgctcgaggg
     8101 tcctttgtgg atcgtgggcc cggagcgcga cccgctcggt ctcgagcttc ctcccggcga
     8161 atccgagccg gcccaggacc ctggcctgga gcgcgccggg ccgggccccg ttccgggtac
     8221 cagcggcctc gatcaggcgg ttggtccggg aagccgccgg gagcaggcac tgatcaagca
     8281 cccgctgggc cgagcctcga cggccttgaa tcccgcggtc ggcggcggcc cggatttccc
     8341 tcgtcttctg gttcagcttt ccgcgctcgt tgcggatcgc ccgccccgga ccggccgcca
     8401 tcgccgcgag cggggcaatc cggtcgcgca ccgctgcctg gctgacccgg cgagtggtac
     8461 ccgccgcccg ggccaggctt gcgcgggcgg ccgggatgtc gatcccgacc acggcttcgg
     8521 ccgcatgggt cggggttgag gcccgaaccg ccgccacgtc gtcgagcagg gtcacgtccc
     8581 gttcgtgacc caccgcggaa atgaccggca ccgcgagcat cacgaccgtg cggcagagat
     8641 cctcgtcgca gaaggcccag aggtcagtca ggctgccgcc gccccggcag acgacgatcg
     8701 agtccacatc cgcgaaagcg gccatgtcac tgaggatcct cgtgatctgc ggagccgcct
     8761 tgcggtcctg gaccggggca aagccccaga ccacctcgcc cttccagccg cgacgctcga
     8821 ggccgaccat cagatcctgc cgggctgccc caccttccgc ggtgatcacc ccgatccgcc
     8881 ggggcagcaa cctgcgcggc aggttcttct gcggctcggt cagcccttcc gcctggaact
     8941 gtttgcgaag cgccgcaaga cgggcgatca ggtccccctc cccgaacgga cggatgtcga
     9001 tggcccggaa actgaaggaa ggcgaagcgt tcttgcctcc ctcgtaataa tcgggcgcgc
     9061 cggtcacgac tacctgcgaa ccgaccttga cggttcccgc tggcaggccg agggcgtcga
     9121 actcccgctt ccacatcgtt cccggcaccc cgccgcgttc gtcgcgcagc tcgaaataga
     9181 tctggacccg tgactggctg aagttcgtga tttcgccgag gatcgtcgct tcggggaggc
     9241 gcttcaggtc ctgcttcagc tggcgggcat agtcgccgac cggggtcggc gcggcatcct
     9301 ctgccgccgc agggttgccc ggttccggaa gctcttcgct catcgctcca gcatgacacc
     9361 gaccgccgac gggcctctgt gacggatcgg cacaggatcg tgcttttggc ttgaaacagg
     9421 ggaaaacccg aagatgccga cggtaaatat gactattacg accagttgac tcgacattct
     9481 ctccggaccc gtgctacctt gcccgtcgtt atttgagttt gtaggtaaag aaagtaacca
     9541 catcaacaag gggggaatcc cgaatgagtt tcagcagttc caagaagacc cggcgcagca
     9601 ttcaggcgct tctcgtcctg gctctcgtag ccttcggcgc gatcatcgcc ggttgcggca
     9661 gcagcgacgg cgacagtggc agcaaggtcc agctggtcgc ctactccacc ccccagaccg
     9721 cctacgaaga aggtctcgag ccggctttca acgagacacc cgaaggtgac ggtgtcgaat
     9781 tctcgaactc gttcggagct tccggcgacc agagccgagc cgtcgaagcc ggccagcccg
     9841 ccgggtacgt caacttcgcg ctcgagaccg acgtgacccg tctggttgat gccggcctcg
     9901 tctcgaagga ctggcagaac aacaagtaca agggcatcgt gaccaactcg gtcgtctcga
     9961 tcgtgacccg tcccggcaac ccggaaggca tcgagtcctt ccaggacatc atcgacaagg
    10021 acgtaagtgt cgtgaccccg aacccgttca cctccggtgg cgcccgctgg aacatcatgg
    10081 ccatctacgg tgccgccctg catgagggca agagcgagca ggaagccctt gacaccgtca
    10141 agcaggtcct ctcgcaggcc gaagtccagc cggccagcgc ccgtgacgcc ctccaggcct
    10201 tcgtcggcgg cgccggtgac gtcctgatct cctacgagaa cgaggcgatc gccgcgcaga
    10261 acgccggcga ggacgtggac tacgtgattc cggattcgac gatcctgatc gagcagccgg
    10321 ccgcagtcac ggtcgatgcc ccgaagtccg cgaccgactt cctcaactat ctctggagcg
    10381 atgccggcca gaagatctgg gccgagcagg gttaccgccc ggtcaacaag agcctggttg
    10441 acgagtccgt ctacccgacc ccgaaggacg agttcacgat cgccgagttc ggtggctggg
    10501 acaaggtcgc cgacgagttc ttcgacgaca agaccggctc gatcgccgag atcgagaagg
    10561 aactgggagt ttccactgaa ggctgaatcc acagccgtag cggcacctcg accccgggcg
    10621 ggcctgcgcc tgcccggggt tgaaaccgcc ttgaccaagg gcctggtcgt cacctacctg
    10681 agcctgatcg tgctgatccc gctggcggcg attgcggcca aggctttctc ggcgggtccg
    10741 gccgagttct gggacgcgat caggcagccg caggcgatcg ccgccctgaa gctgaccgcg
    10801 atctgctccc tgatcgtcgt ggcgatcaac gcggtgatgg gcaccctgat cgcctgggtc
    10861 ctggtccggg acgagttccc cggcaagagc atcgtcaact cgatgatcga cctgcccttc
    10921 gccctgccga ccatcgtcgc cagcctcacc ctgctcacgc tctacggcaa tgacagcttc
    10981 ctcgggattc acgccgccta cacaaagttc gcggtgatca tcgccctgct cttcgtgacc
    11041 cttcccttcg tggtgcgggc cgtccagccg ctgctgctgt cccttgaccg tgacatggag
    11101 tccgccgccg aatcgcttgg cgccggcaag gtccgcatct tcacgcagat catcttcccc
    11161 aacctgctgc ccggcatcgc cgccggcgtc gcactcgcct ttgcccgcgc gctcggtgaa
    11221 ttcggcgccc tcgtgctgat ctcgggcaat gtgccctaca agaccgaggt cgcctcggtc
    11281 ttcatccgcg gccagatcga atccgaccag gtggtcggtg cggccgccgt ctcggtcgtg
    11341 ctcctggtct tctcgatgtg catgctggcc ttcgtcaacc tgatccagaa atggggtctg
    11401 cgtcatgacc gctagtggcc gactctccaa ata
//
LOCUS       DAOQKN010000072        11414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_2663_length_11414_cov_6.080377, whole genome shotgun sequence.
ACCESSION   DAOQKN010000072 DAOQKN010000000
VERSION     DAOQKN010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 11414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11414
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2663_length_11414_cov_6.080377"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(318..974)
                     /locus_tag="PLJ64_06425"
     CDS             complement(318..974)
                     /locus_tag="PLJ64_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93149.1"
                     /translation="MNEDALPSVSSMDALSRLRRFMAPRKFVSSRRELTEAGFDASRI
                     KNWLRHGRLVLLLRGVYSFGRDVGSRESAWRAALLAAGPGSVLNGRSACELWGMITPT
                     GEIPRYIEVAVTAGEARRLRGTSPALSRTWINIVKRQFSPEDISRVNGLEVTSALVAL
                     VDFAAKASPREVRFAFLEGCRLGLVQKRDVIRLRRLMRGRRGVEKLNPLVALWDEIPG
                     "
     gene            complement(1304..2095)
                     /locus_tag="PLJ64_06430"
     CDS             complement(1304..2095)
                     /locus_tag="PLJ64_06430"
                     /inference="COORDINATES: protein motif:HMM:NF025389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4239 domain-containing protein"
                     /protein_id="HNC93150.1"
                     /translation="MSTLLTCLAILVAGCLVTGISILLVRKFVPEHLRGSEERREPVF
                     AFAGVLYALVIGFVLAYALDGYQTAETAAGTEADSVSSLSRAATLFDAESRDRIGHEL
                     ICYSRAVVDDEWPAMEHGRRSELATAATDRLFQSFGKLGRNSADNSALSTSLERVSDL
                     QKARATRLLKSDTFLPTMFWVFMVVGGFMLILFATVLKGREPIRGQVMFILPVSLLLI
                     CSIYLVYVFEQPFRGPNEIKPRAMERALVSVTEFIPDPRADRPCP"
     gene            complement(2095..3351)
                     /locus_tag="PLJ64_06435"
     CDS             complement(2095..3351)
                     /locus_tag="PLJ64_06435"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HNC93151.1"
                     /translation="MKSSVPFRAAVAAVVLLAAVALAIDGCGKDEETETTRTLRIYVS
                     QPLKGDRGGEDMLRAVEIANEDEGGTIGPARIEIVGMNDSDESGNWVASLVRRNARRA
                     AADDSAVAYIGDFDSGATEVAMPILNRAGMLQVSSSATAVKLTKPLPELGEHIRPTGI
                     RTFGRVVPNDNVQAAALALFMDQESVNEVFVVDDGDTYGEGLRFRFEKVARHTGIEIT
                     GGATVTRSIQIGDVAKRVIGSGADALLFAGSNLDLARDLFETVHREDNFIKLFGGDGL
                     SLSSFLGSLGDTGLDTYITAPQLPAGNYARSGESFLRGFQERYGRPAEPMAVFAYEAG
                     HVVIDSIRKGTRGDIATEPIDALRQNTRNAFFDTSERASPLGSYSIDTNGDTTLTFYG
                     AYRVEDGQLVLGRTIDVPPSLLREDE"
     gene            complement(3416..3985)
                     /locus_tag="PLJ64_06440"
     CDS             complement(3416..3985)
                     /locus_tag="PLJ64_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HNC93152.1"
                     /translation="MTDWVFGYGSLVTDLVTSSDENGFEWFWANLGNHRRDWGAGMEN
                     AAADADWKYYIDSRGTRPDIVVAFLDAYPHSGSSINGVVFAVDESRLREIDRREVRYS
                     RVDVTDLVTTAGWGDGRVWTYRGSEKSREVALRGRREDRAFVSMDYAAHCRHGFSVGG
                     EQALHQFQLSTDPLDLPLRELTMVLSDPG"
     gene            complement(3982..4899)
                     /locus_tag="PLJ64_06445"
     CDS             complement(3982..4899)
                     /locus_tag="PLJ64_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93153.1"
                     /translation="MVGEPPRPKQPPGRIGTLARLFHPKALNRKHPHVSDRLRTFMAT
                     LTFLELVLIFVLLIVASTVLLGLTFWWLAPSQLYNSYWDALGSAFFTLIGMPPGYLDP
                     AELSGRFKVAVGAAGLFSLLLPALFLGAIVFRLFVRLRIFVLKERPTLTRKKVRGTEG
                     WYLAIRLYSSTAILIVDIDFKVYLRMKSSNGLLLSNEEIRVTNATWPIADTHVPYTLY
                     LPVDDGDLDANEHLISLQGHDLETCESAIIHIRGASPQLASEVNETHTLKVPLDLSPA
                     DFGGVQVLYDGRGPDWVGWDEFEADRPLQ"
     gene            5123..5695
                     /locus_tag="PLJ64_06450"
     CDS             5123..5695
                     /locus_tag="PLJ64_06450"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNC93154.1"
                     /translation="MNQNSLHPGFTPNVVAIVGNQRTDSRTLRLASGIADGLAAQLGD
                     TPAVAIDLVNLPLENGGFSPGLNDLIRDSRVVIAASPTVMGSYSGLLKIFLDGLPEAA
                     LLDKTGIPLMIARQARHALAADVHLRPALLELGATCPTPSLVALESELASLGADGLVT
                     AWLERSGNWLQLSDGSAAGCSPRRHRHLTT"
     gene            complement(5637..6818)
                     /locus_tag="PLJ64_06455"
     CDS             complement(5637..6818)
                     /locus_tag="PLJ64_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93155.1"
                     /translation="MSNARFSSLSRIHLLGLGLLALITLALLAAPSKSRAADQPFKID
                     FDQSSMQIGLLSDLPLGDLATTGSLDGTIDEEGNVTIPKGNFILPEIGITDPIAIKVF
                     MGVESDATGTYDANTGELVLNAKAGVWFSVDLQALLSTLGTLGVQLPGGSGIIDTILG
                     QVTDLTCGFSPMDVTFTTGETSQGSGAPFSEGPLGPGALTAEWSQLGPFAGKTKILGL
                     IDACQAIKMYAPTLLESALGSSGIDLASLGLGDIDLASLLDNLDNLNLGPSSLTLTRT
                     LDESPDPPAAEPVLKMSVKGPNRKVKAGSKAEFTVKVKNSGQGDATGVRVCLKSSGPV
                     IVQGSRCRTLGKVMAGATQTRHFKVKAKKGKKKSAKISFSLKSSNGDDASASTRLLIR
                     H"
     gene            6947..7879
                     /locus_tag="PLJ64_06460"
     CDS             6947..7879
                     /locus_tag="PLJ64_06460"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HNC93156.1"
                     /translation="MSNAQFEKETDKKGAFVRQTSAFRERVTADGSSGFPAEPGRYHL
                     YVCYACPWASRTIIFRTLKGLEEVISMTAVDPVRDDQGWKFFADDPDPINGYEYLLEA
                     YLRTDPDFSDRVTVPVLWDKETNRAVNNESSEIIRMLTSEFNQWAKNPDLDLYPEPLR
                     AEIDELNDRIYNSINNGVYRAGFATTQEAYEEAFIELFEALDLMDDRLGESRFLTGGE
                     ITEADWRFFVTLIRFDAVYVGHFKCNQRRIADYDNLAGYLRDLYQQPGIRETVNMDHI
                     KRHYYITHPRINPTQIVPMGPVLDLESDPARSHL"
     gene            7902..9026
                     /locus_tag="PLJ64_06465"
     CDS             7902..9026
                     /locus_tag="PLJ64_06465"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNC93157.1"
                     /translation="MSSENSAPRWHWFLPTSGDGRNVTNVIDQLGQTSMSRPADVEYL
                     AEVAKAAERAGFEGVLTPTGSGCEDAWVTCAAVAQHTEKLKFIVAFRPDAIAPAWAAH
                     QSASFQRLTGGRLLLNVVTGGDPVEQKSFGDHLDKEARYRRTGEFLEIVKRSWSGETF
                     DHEGEFFHLEKGGLRLGFGEVDPPRVYFGGASPPALEVAARHADTWLTWGEPVDTVAG
                     RVAPVREKAEAAGREISCGLRIHVISRDTEEEAWLEADRILEAIDPEQVAAAREKFAR
                     MESTAQARMTDLSHSSEGAALDRDALVIGPNLWAGVGLVREGAGTAIVGSHQQVAERL
                     LEYRDIGIDEFILSGYPHLEEALNFGENVRPLLDAATVFA"
     gene            9046..10152
                     /locus_tag="PLJ64_06470"
     CDS             9046..10152
                     /locus_tag="PLJ64_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93158.1"
                     /translation="MRLINNPVRRFDFVQKLEWFLLSAVTMILIIRTQLWLTHYPQLG
                     GGGLHIAHLLYGGLFMVIAIWLGQIYLNRWGRTVQAILGGIGFGFFIDELGKFITEDN
                     NYFFKPAAGIIYLIFIFMFLAIRELSRRQSLDPRSALANALSLLPGTAIGEYRKDEAE
                     RAGRLLDMADQSDPMVGKAREMFRQAEVVPGRPPSRLTGTVNRIHGWIRGLTQRRHFE
                     GIVIAVVIIWALSSLSSVVAIDLDLGGVQDHQGPEVNSGVLGSLESLSALVSVVLVFI
                     GAWAMVRRDHARAYRYFGRALLVSILVTRVFVFIESQFAAVFGLALDLVLFAAISELA
                     SQDEKTRFRFGGLGQADLAEAGSTPEKPSRSQNP"
     gene            10253..>11414
                     /locus_tag="PLJ64_06475"
     CDS             10253..>11414
                     /locus_tag="PLJ64_06475"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HNC93159.1"
                     /translation="MRSIRVYLAALAALIGGLALIGGMATTVSAKPKKLKSAPKVTSK
                     ATYTARGSVGDAYVKNAEEGQTLLLVNANNKVVRTGKADSFGSKVFYDVAPGKTFTVR
                     TPKGKGALGTKPFKVLKPGANPPQSFYQDSSHELHQGLNYVKMRDGTEIAMTVRLPLG
                     KTITDGPFPTYIEYSGYQTAAPHDLLSVFLGGGGSDDLAPASSTAVGSLIGPLLDFAT
                     VSVQMRGSGCSGGAFDLFGWPTTYDGYDAVETVAAQDWVQGKVGMGGISFSGISQLFT
                     AGTRPPHLAAVSPLSVTDDVYYGTGFPGGIFNNGFAFSWITERAEDAEPAPEGGQTWA
                     KIMTTTGDPEVDEPLRSEQKQHCIDNQKMRLQTRNYNHLIETNPYRTPNLFAK"
BASE COUNT         2137 a         3685 c         3604 g         1988 t
ORIGIN      
        1 ctcgagtaca acgaccgcca caacctcgag gacaagaccg tccccagccg caagctcggc
       61 ccgatccccg aagacaagca gcaagacctc ggcctgacca agtccggaaa agtagcccaa
      121 ccggacaact aaccacccaa cctcccgacc tcgggccagg aacccccgcg agatccgtct
      181 ccgggcacga atccccgcga gatcccgttt ggcgccgaat agcgcaccca actaaccggg
      241 ctgcctcacg acctccagca cagttccccg cgagatccct ttttgcgccg aatagcgcac
      301 ccaactccga cttcgatcta acccgggatc tcgtcccaaa gggcgaccag cgggttcagc
      361 ttctccacgc cccttcggcc ccgcatcaac cgtcgcaacc ggatgacatc ccgcttctga
      421 accaggccga gccgacaccc ttcaaggaag gcgaatcgaa cttccctcgg ggaagccttc
      481 gccgcaaaat cgaccagggc cacgagggct gaggtgacct caagcccgtt cacccgactt
      541 atgtcctccg ggctgaactg tcgcttgacg atgttgatcc aggtccggct cagagcagga
      601 gaagttcctc gcaaccgacg agcttcgccg gccgtcaccg ccacctcgat gtagcggggg
      661 atctcaccgg tcggagtgat cattccccac aattcgcagg cacttcggcc gttcagaacc
      721 gagcccggcc ccgccgcgag caaggctgcc ctccaggccg actcccgcga cccgacgtct
      781 cgaccaaacg agtagacccc tcgaagcagc aagaccagac ggccgtgtcg aagccagttc
      841 ttgattcggg aggcgtcgaa tccggcctcc gtgagctcgc ggcgactgga cacgaacttg
      901 cggggggcca tgaagcggcg gaggcggctg agcgcgtcca ttgagctgac cgacggaagg
      961 gcatcctcat tcattgcccg acggtgtcaa agaaaagctc acatgtgttt cgacaggtgt
     1021 gacgattctg caacgtgccg gtgcacggaa cggagtgtgg gcgcgagagc aggcaggttg
     1081 atcgttcagc tcgagatcgg acttgggtgc gctatctggc gaggagtgac gtttggtgga
     1141 acccggatcg ctcgagatct gagttggggg cgctatttgg cgaaaagtga catctcgtga
     1201 gaagcggcat ctcgtgagaa gcgattccgg gcggcgggag gcggccagcg gcgggcggcc
     1261 agctggggga agcgtctggc ggcgccggaa cggcggcgga ggatcagggg caggggcggt
     1321 cggcgcgggg gtcggggatg aattcggtta ccgagaccag ggccctttcc atcgcccggg
     1381 gcttgatttc gttcggtccg cggaagggct gctcgaaaac gtagaccagg tagatcgagc
     1441 agatcaggag cagggagacg ggcaggatga acatgacctg gcctcggatc ggctcacgac
     1501 ccttgagcac ggtggcgaaa aggatcagca tgaagccgcc gaccaccatg aagacccaga
     1561 acatcgtggg caggaaggtg tcggatttca gcagcctggt ggcccgggcc ttttgcaggt
     1621 cgctgacccg ctccagggag gttgaaaggg ccgagttgtc ggcgctgttt cgtcccagct
     1681 tgccgaagga ctgaaagagc cggtcggtgg cggcggtcgc gagctcggaa cggcggccat
     1741 gctccatcgc cggccactcg tcatcgacaa cggcgcggga gtaacagatc agttcgtggc
     1801 cgatccggtc ccggctctcg gcatcgaaaa gagtagccgc gcgggaaagg gaactgaccg
     1861 aatcggcttc ggtgccggct gcggtctcgg cggtctggta gccgtcgagc gcgtaggcga
     1921 ggacgaaacc gatcaccagg gcgtagagca cgccggcgaa agcgaagacg ggctcgcggc
     1981 gctcttcgct gcccctcagg tgttcgggga cgaacttgcg gaccagcagg atcgagatcc
     2041 cggtgaccag gcatcccgcc acgaggatcg ccaggcaggt aagcagggtg ctcatcactc
     2101 gtcctcccga aggagagaag gtggcacgtc gatcgtccgg ccgagcacca gctggccgtc
     2161 ctcgacccgg taggcgccgt agaaggtaag ggtggtgtca ccgttcgtgt cgatcgaata
     2221 gctgccgagc ggcgaggcgc gctcggaggt gtcgaagaag gcgttgcggg tgttctgccg
     2281 gagcgcgtcg atcggttcgg ttgcgatgtc gccgcgggtg cccttgcgaa tcgaatcgat
     2341 cacgacatga cctgcctcat aggcgaagac ggccatcggc tcggccggtc gtccataccg
     2401 ttcctggaaa ccacgcagga aggattcacc gctccgcgcg taattgccgg ccgggagctg
     2461 aggcgccgtg atgtaggtat cgagcccggt gtcgccgagc gatccgagga aggaggaaag
     2521 cgagagaccg tcaccaccga agagcttgat gaagttgtcc tcgcggtgca ccgtctcgaa
     2581 gaggtcacgg gccagatcaa ggttcgaccc ggcgaagagc agggcgtcgg ccccggatcc
     2641 gatgactcgt ttcgcgacgt cgccaatctg gatcgaacgg gtcacggtcg cgccgccggt
     2701 gatctcgatc ccggtgtgcc gggcaacctt ctcgaagcga aaacgcagtc cctcgccgta
     2761 ggtgtcgccg tcatcgacca cgaacacctc gttgacgctc tcctgatcca tgaacagggc
     2821 cagggccgcg gcctggacat tgtcattggg aaccacccgg ccgaaggttc tgatcccggt
     2881 cggccgaata tgttcaccga gttcgggcag gggcttggtc agcttgacgg cggtggcgct
     2941 ggagctgacc tggagcatcc cggcgcggtt gaggatcggc atcgcgacct cggttgcgcc
     3001 ggaatcgaaa tcaccgatgt aggcgacggc ggagtcgtcg gcggcagcgc gccgcgcgtt
     3061 gcggcggacc agcgaagcga cccagttgcc gctctcgtcg gaatcgttca tgccgacgat
     3121 ctcgatccgg gccgggccga tcgtcccgcc ctcgtcctcg ttcgcaatct cgaccgccct
     3181 aagcatgtcc tcgccgccgc ggtcaccctt cagcggctgg cttacataga tccgcagggt
     3241 gcgggtcgtt tcggtctctt cgtccttgcc gcagccgtcg atggccagcg caaccgcggc
     3301 gagcagaact acggccgcga ccgcggcccg gaacgggaca cttgacttca agatgccgga
     3361 ctattccagt tcgtcggccc tcacgttgaa accgaaccgg gctgacgcgc aagagtcaac
     3421 caggatccga aagaaccatc gtgagttcgc ggagtggcag atcgagggga tccgtgctca
     3481 attgaaactg gtgaagtgcc tgctcacctc ccacggagaa tccgtgccgg caatgcgctg
     3541 cataatccat gcttacgaag gcccggtctt ctcgccttcc tcggagtgcg acttcgcgag
     3601 atttctcgct cccccggtag gtccagaccc gaccgtctcc ccaaccggcc gtcgtgacca
     3661 gatcagtcac gtcgacgcgg gagtacctca cttcgcgccg atcaatttcc cggagacgac
     3721 tctcgtctac ggcaaatacg actccgttga tgctggaacc ggagtggggg taagcgtcga
     3781 gaaaggcgac cacgatgtca gggcgtgtgc ctcgagagtc gatgtagtac ttccaatcgg
     3841 catcggcggc agcattctcc atgccggcac cccaatcccg ccggtggttg ccaagatttg
     3901 cccagaacca ctcaaagcca ttctcgtccg aacttgtgac caggtcggtg acgagcgatc
     3961 cgtagccgaa gacccagtcg gtcattgcaa cggtcggtct gcttcgaact cgtcccagcc
     4021 cacccaatcc gggccacggc cgtcgtagag aacctggacc ccgccaaaat ccgccggaga
     4081 gagatccaac ggaaccttca gcgtgtgcgt ttcattcact tccgacgcaa gctgtgggga
     4141 cgcgcctcgg atgtggatga tcgcgctttc gcaggtctct agatcgtgac cctgcagtga
     4201 aatgaggtgc tcgttggcat caagatcgcc gtcgtccacg ggaaggtaaa gggtgtaggg
     4261 gacatgagtg tcggcgatgg gccaggtggc gttggtcacc cggatctctt cgttcgagag
     4321 cagtaatccg tttgagctct tcatccgcaa gtagaccttg aaatcaatat cgacgatcag
     4381 gatcgctgtc gacgagtaaa gacggatcgc cagataccag ccctcggtgc ctctcacttt
     4441 ctttcgggtg agtgttggtc tttccttgag gacgaagatg cgcagcctga cgaaaaggcg
     4501 gaagacaatg gctccgagga aaagggccgg caaaagcaac gagaagagtc cagccgcccc
     4561 tacggcgacc ttgaatcttc cactcaattc tgcagggtcc agatagccgg gtggcatgcc
     4621 gatcagggtg aaaaaggctg atccaagcgc gtcccaataa ctgttgtaga gctggcttgg
     4681 agcaagccac cagaaggtga gtcccagaag gaccgtcgat gcgacgatca acagaacgaa
     4741 gatcagaacg agctccagaa aagtcagagt cgccatgaat gtcctcaagc ggtcggaaac
     4801 atgaggatgc ttgcgattca gggccttcgg atggaaaagg cgggccaggg tcccaattcg
     4861 tcctggcggc tgcttgggcc ttggcggttc gccaaccatt gtccgatcct attcgggaat
     4921 tgagcgggca gactcctgcc cattgctggt cttctggtcg atcatgtagt atcttgattt
     4981 attgactaga caaactcata aatgtctaag tcgactccgc tgggcggtgg gctagacggc
     5041 ccggctggac gcaaggcaag ggggccgggc cgaaaggtcc ggcccccgac cctccttccc
     5101 ccggtcaaga caggacgatc cgatgaacca gaactccctc catcccggat tcacccccaa
     5161 cgtcgtcgcc atcgttggca accagcgtac ggacagccgc accctcaggc tggcctccgg
     5221 catcgccgac gggctggcgg ctcagctcgg cgacaccccg gcggtcgcga tcgacctggt
     5281 caacctgccg ctggaaaacg gcggcttcag cccgggactg aacgacctga tccgggactc
     5341 ccgggtcgtg atcgcggcca gcccgaccgt gatgggcagc tactcgggcc tgctcaagat
     5401 ctttctcgac ggcctgcccg aggcggcgct gctcgacaag accgggatcc ccctgatgat
     5461 cgcgcgccag gcccgtcacg ccctggcggc cgacgtccac ctgcgaccgg ccctgctgga
     5521 acttggcgcg acctgcccga ccccgtcgct ggtcgcgctc gagtcggagc tcgcctcact
     5581 gggagccgac ggtctggtca ccgcctggct ggaacggagc ggaaactggc tgcagctcag
     5641 tgacggatca gcagccgggt gctcgccgag gcgtcatcgc catttgacga cttgagcgag
     5701 aagctgatct tcgcggactt cttcttgccc ttcttcgcct tcaccttgaa atgacgggtc
     5761 tgggtggcac cggccatcac cttgccgagg gtccggcagc ggcttccctg gacgatcacc
     5821 ggcccggaag acttcaggca gacccggacg cccgtcgcat caccttgacc cgagtttttg
     5881 accttgacgg tgaactccgc cttgctgccg gccttgacct tgcggttcgg tcccttgacg
     5941 ctcatcttca gcaccggctc cgccgcgggc gggtccgggc tctcgtcgag tgtccgggtc
     6001 agggtgaggg agctggggcc gagattcagg ttgtcgaggt tgtcgagcag ggaagccagg
     6061 tcgatgtcac cgagcccgag gctggcgaga tcgatgccgg agctgccaag ggcactctcg
     6121 agcagggtgg gtgcgtacat cttgatcgcc tggcaggcgt cgatcagacc gaggatcttg
     6181 gtcttgcctg cgaagggccc gagctgcgac cactcggcgg tcagcgcacc gggcccgagc
     6241 ggcccctcgc tgaacggggc gccggaaccc tgcgaggtct caccggtggt gaaggtgacg
     6301 tccatcggcg agaacccaca ggtcaggtcg gtcacctggc caaggatcgt gtcgatgatt
     6361 cccgaacccc ccggcagctg gactcccagc gtgccgagcg tggagagcag ggcctggagg
     6421 tcgaccgaga accagacccc cgccttggcg ttgaggacta gctcaccggt gttggcatcg
     6481 taggtcccgg tggcgtcgct ctcgacaccc atgaacacct tgatcgcgat cgggtcggtg
     6541 atgccgatct cggggaggat gaagttgccc ttggggatgg tgacgttgcc ctcctcatcg
     6601 atcgtcccat cgagggagcc ggtggtcgcg aggtcgccga gcggcagatc gctgagcagg
     6661 ccgatctgca tgctgctctg gtcgaagtca atcttgaaag gctgatccgc agcccgggac
     6721 ttcgaggggg cagccagcag ggcgagggta atcagggcaa gcaggccgag gccgaggagg
     6781 tggatcctcg aaagactgga aaaacgcgcg ttggacactt cttctccctt gtttccgccc
     6841 gctccctggg catcgacgag ccggaccggt tccggcgctg atcacactat aaacgcgaat
     6901 ccgtcgcccg gcttgttttc cggggtcgat gggtaaagtc ggcaacgtga gcaatgcaca
     6961 gtttgagaag gaaaccgaca aaaaaggcgc gttcgtacgc cagacgagcg ccttcaggga
     7021 gcgggtaacc gccgacggct cttcaggctt tccggcggaa cctggcaggt accacctgta
     7081 cgtctgttac gcctgcccct gggcctcgcg gacgatcatc ttccgcaccc tgaaagggct
     7141 ggaggaagtg atctcgatga ccgccgtcga tccggtccgg gacgatcagg gctggaagtt
     7201 cttcgccgac gatcccgacc cgatcaacgg ctacgagtac ctgctcgagg cctaccttcg
     7261 caccgacccc gacttcagcg accgggttac cgtgcccgtg ctctgggaca aggagaccaa
     7321 ccgcgcggtc aacaacgaga gcagcgagat catccgcatg ctcacctccg agttcaacca
     7381 atgggcgaag aaccccgacc tcgaccttta ccccgaaccg ctccgggcgg aaatcgacga
     7441 actgaacgac cggatctaca actcgatcaa caacggggtc taccgggccg gcttcgccac
     7501 aacgcaggag gcatacgagg aggctttcat cgagctcttc gaggcgcttg atctgatgga
     7561 cgaccggctg ggggagagtc gcttcctgac cggcggcgag atcaccgagg cggactggcg
     7621 cttcttcgtc accctgatcc gctttgacgc cgtctacgtc ggccacttca agtgcaacca
     7681 gcgacggatc gccgactacg acaacctcgc cggctacctg cgtgacctct accagcagcc
     7741 cggcatccgc gagacggtca acatggacca catcaagcgg cactactaca tcacccaccc
     7801 gcggatcaac ccgacccaga tcgtcccgat gggccccgtc ctcgacctcg agagtgaccc
     7861 cgcccgcagc cacctctgat tgtcgagtag gtttatcgac tatgagctct gaaaacagcg
     7921 cgccccgctg gcactggttc cttcccactt cgggggatgg acgcaacgtg accaacgtga
     7981 tcgaccagct cggtcagacc tcgatgtcgc ggccggccga tgtcgaatac ctggccgagg
     8041 ttgccaaggc agccgagcgg gccggcttcg aaggtgtgct gaccccgacc ggttccggtt
     8101 gcgaggacgc ctgggtgacc tgcgccgcgg tcgcccagca caccgagaag ctcaagttca
     8161 tcgtcgcctt ccggccggat gcgatcgcgc cggcctgggc cgcgcaccag tcggcctcct
     8221 tccagcggct gaccggcggc cggctgctgc tcaacgtggt caccggtggc gacccggtcg
     8281 agcagaagtc cttcggcgac cacctcgaca aggaggcccg ttaccgccgc accggcgagt
     8341 tcctcgagat cgtcaagcgc tcctggtcgg gcgaaacctt tgaccacgaa ggcgagttct
     8401 tccacctcga gaagggcggc ctgcgcctcg ggttcggtga ggtcgatccg ccccgggtct
     8461 atttcggcgg ggcctctccg ccggccctcg aggtcgccgc caggcacgcc gacacctggc
     8521 ttacctgggg cgagccggtc gacacggtgg ccgggcgggt cgcgccggtc agggagaagg
     8581 cggaggctgc cggtcgcgag atcagttgcg gactgcgcat tcacgtgatc agccgtgaca
     8641 ccgaggaaga ggcctggctc gaggccgacc gcatcctcga ggcgattgat cccgaacagg
     8701 ttgccgccgc gcgcgagaag ttcgcccgca tggaatcgac cgcccaggcc cggatgaccg
     8761 acctcagcca ctcttccgaa ggagccgcgc tcgaccggga tgccctggtc atcggaccca
     8821 acctctgggc cggggtcggg ctggtccgcg aaggggccgg caccgcgatc gtcggcagcc
     8881 atcagcaggt tgccgagcgc ctgctcgaat accgcgacat cggcatcgac gagttcatcc
     8941 tctccggcta tccgcatctc gaggaagcgc tcaacttcgg cgagaacgtg cgcccgctgc
     9001 tcgacgccgc gaccgtcttc gcctgagcga agctacagtc agcccgtgcg gctgatcaac
     9061 aatccggtca ggcggttcga cttcgtccag aagctcgaat ggttcctgct gtcagcggtg
     9121 acgatgatcc tgatcatccg cacgcagctc tggctgaccc actacccgca gctgggcggc
     9181 ggtggtcttc acatcgccca tctgctctac ggcggcctct tcatggtgat cgcgatctgg
     9241 ctcggccaga tttacctgaa ccgctggggc cggaccgtcc aggcgatcct cggcgggatc
     9301 ggtttcggct tcttcatcga tgaactgggc aagttcatca ccgaggacaa caactacttc
     9361 ttcaagccgg ccgccggaat catctacctg atcttcatct tcatgttcct ggccattcgg
     9421 gagctctccc gccggcagag cctggacccc cggtcggcgc tggccaacgc gctttcgctg
     9481 ctgccgggca ccgcgatcgg ggaataccgg aaggacgagg ccgagcgggc cggcaggctg
     9541 ctggacatgg ccgaccaatc cgatccgatg gtcggcaagg caagggagat gttccgccag
     9601 gcggaagtgg ttccgggccg gccgccttcg cgactgaccg ggaccgtgaa ccgcatccac
     9661 ggctggatcc gcggcctcac gcagcgccgt catttcgagg ggatcgtgat cgcggtggtg
     9721 atcatctggg cgctctcctc gctttcctcg gtggtcgcga tcgatctcga cctgggcggg
     9781 gtccaggacc atcagggacc ggaagtcaac tccggggtcc tcggttcgct cgagtctctc
     9841 tcggccctgg tctcggtcgt cctcgtcttc atcggcgcct gggcgatggt ccggcgcgac
     9901 cacgcccggg cctaccgtta cttcggccgg gccctgctcg tttcgatcct ggtgacccgg
     9961 gtcttcgtct tcatcgagtc ccagttcgcc gcggtcttcg gcctcgccct ggatctggtc
    10021 ctcttcgcgg cgatatccga gcttgccagc caggacgaaa agacccgatt ccgctttggc
    10081 gggctgggtc aggccgatct ggccgaagcc ggttcaacac ctgagaaacc ctcccgttca
    10141 cagaatccct gatttcccgt atagtttccc gggcagggaa tgggagaatg actccaacgg
    10201 gggacatgag gcgcctccag gcaaagtgaa attagggatg gaggaacgca gcatgcgcag
    10261 tattcgggtg tatctggccg ctctggccgc attgatcggg gggcttgccc tgattggcgg
    10321 gatggccacg accgtcagtg ccaagccgaa gaagctcaag tccgcgccga aggtaacttc
    10381 gaaggcgact tacacggctc gcggcagcgt cggtgacgcc tatgtcaaga acgccgagga
    10441 gggccagacc cttctgctgg tcaatgcgaa caacaaggtt gttcgcaccg gcaaggccga
    10501 ttcgttcggc tcgaaggtct tctatgacgt tgctcccggc aagactttca cggtccgcac
    10561 cccgaagggc aagggagcgc tcggcaccaa gccgttcaag gttctgaagc ccggcgccaa
    10621 cccgccccag tccttctacc aggactccag ccacgaactt caccagggcc tcaactacgt
    10681 gaagatgcgc gacggcaccg agatcgcgat gaccgtgcgg ctgccgctcg gcaagaccat
    10741 caccgacggt ccgttcccga cctacatcga gtactcgggc taccagaccg cggccccgca
    10801 tgacctgctc agcgtcttcc tcggcggcgg cggaagcgat gatctcgctc cggccagctc
    10861 gaccgcggtc ggctcactga tcggtccgct gctcgacttc gccacggttt ccgtccagat
    10921 gcgcggttcc ggctgttccg gcggggcctt cgacctcttc ggctggccga ccacctacga
    10981 cggctatgac gcggtcgaga ccgtcgccgc ccaggactgg gtccagggca aggtcggcat
    11041 gggcggcatt tccttctccg gcatttccca gctcttcacc gccggcaccc ggccgcctca
    11101 cctggccgcc gtctcacccc tttcggtgac cgacgacgtc tactacggca ccggtttccc
    11161 gggcggcatc ttcaacaacg gcttcgcgtt cagctggatc accgagcggg ccgaggatgc
    11221 ggaacctgcc cccgagggtg gccagacctg ggccaagatc atgaccacga ccggtgaccc
    11281 ggaagtggac gagcccctgc gcagcgagca gaagcagcac tgcatcgaca accagaagat
    11341 gcggctccag accaggaact acaaccacct gatcgagacg aatccgtacc ggaccccgaa
    11401 cctcttcgcc aagc
//
LOCUS       DAOQKN010000073        11385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_2671_length_11385_cov_5.518447, whole genome shotgun sequence.
ACCESSION   DAOQKN010000073 DAOQKN010000000
VERSION     DAOQKN010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 11385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11385
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2671_length_11385_cov_5.518447"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..408)
                     /locus_tag="PLJ64_06480"
     CDS             complement(1..408)
                     /locus_tag="PLJ64_06480"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNC93160.1"
                     /translation="MAELEGRSLEIVKEGANYAVVAVPTDDGSVQSVIVWAHADDDGN
                     VILNSAEGRAWPQNLRKAGRATVTMMADGNPYEYVSIRGRLIGDTQEGADDDINMLAK
                     KYIDADEYPFRQPGEVRVTFTLEPEKVNYLKQG"
     gene            complement(445..1482)
                     /locus_tag="PLJ64_06485"
     CDS             complement(445..1482)
                     /locus_tag="PLJ64_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC93161.1"
                     /translation="MNDQATQLESTSTGEALVQVKDLNKHFPLSGNSLLGRGKGAVRA
                     VDGVSFELKKGETLGLVGESGCGKSTLARSLLQLLRPSSGSVRFRGVELTEISNRKLR
                     PIRRDMQMIFQDPYTSLNPRKRVGQIIGEPLRIHGLAEGREIERRVREKLDQVGLSGS
                     YYDRYPHEFSGGQRQRIGIARALAPEPSLIVADEPVSALDVSIQGQIVNLMADLQREL
                     ELTYLFVAHDLGVVRQVSDRIAVMYLGKIVEIGPAELVYSKPIHPYTASLLAAVPIPD
                     PRTNAQRERRPLEGDVPSPSDPPSGCRFHTRCPYATEVCEQQEPALIDYGQGHFAACH
                     HPLGRPDPAGE"
     gene            complement(1479..2510)
                     /locus_tag="PLJ64_06490"
     CDS             complement(1479..2510)
                     /locus_tag="PLJ64_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC93162.1"
                     /translation="MSEPLLSVRDLEVSFRTEGGLLQAVGGVSFDLQRGETLSIVGES
                     GCGKSVTAMTLMGLTSGPNTEFKGSVKLDGQELLGASNEVMRPIRGDRMSMVFQNPMT
                     SLNPVHRIGNQIVEQIRAHRDVSKKEAMARAIELLDAVGVPDAAARARAYPHEFSGGM
                     RQRAMIAMALSLEPEILIADEPTTALDVTIQAQILELLKQLNRDFNLTVILITHDLGV
                     VADIADRVGVMYAGRLVETGTLDEIFYDPQHPYTWGLLGSVPRIESTRQARLAQIEGQ
                     PPSLIDPPTGCRFRDRCPHAFDKCGVEPDLVSRVADPSHLDRCWLEVSERPALRQLPN
                     GLIGLASPE"
     gene            complement(2507..3490)
                     /locus_tag="PLJ64_06495"
     CDS             complement(2507..3490)
                     /locus_tag="PLJ64_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC93163.1"
                     /translation="MLAFALRRLGSMVLVLFAVSVLTFLIFNVLPSGDPAVRMAGKQP
                     TETQIEAIREEWGFNEPVYVQYVKTMENLFSGNMISYFNQLNVLEEIKKGIPRTFALA
                     IGAAIIWMAFAVALGVWGAMRAGGISDGLLNFFALVGISMPVFWLGALMNQYLGFELT
                     KKLGFELFPNGGYVEFSQSPWEWFHHLILPWIALSVLFIGFYSRVLRANILETMDEDY
                     VRTARAKGLSERRVMLSHVLRNSMISVVTLWGLDFAVVVGGGAILTETVFNLQGVGQY
                     AADAIGQLDQPPIMAVTMFGAFFVVFFNAVIDIVYAALDPRIRLQDGDTAL"
     gene            complement(3490..4530)
                     /locus_tag="PLJ64_06500"
     CDS             complement(3490..4530)
                     /locus_tag="PLJ64_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC93164.1"
                     /translation="MSGATDRPVSDLQEFSEPPAVTQEGVAGESPWRLAVRRMRKRRF
                     ALFMGAVFVLLVLTMAAAPLWAEHVAHTTPSANHLSDQIEVDGEKVNVVGLDGVPIGP
                     TWQGQFFLGADTNGRDVAVRLLYGGRNSLIIGLGAALITLVLGLVTGLLAGYFRGWTD
                     SILSRIMDVLWAFPVILLGIALGTSLALGGLKLGPVSVSGGSLAIPIFVIAIVYIPYL
                     ARPIRGEVLSLREKEFVEAARAQGMGSFRLITSEILPNVLSTGLVFFPLLVANAILLE
                     AALSFLGAGVQPPEPSWGTMIDEGVARIVTAPALAIVPGMMIVLTVLSLNVFGEAIRD
                     SLDPKAKVRIEH"
     gene            complement(4569..6227)
                     /locus_tag="PLJ64_06505"
     CDS             complement(4569..6227)
                     /locus_tag="PLJ64_06505"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNC93165.1"
                     /translation="MLAVVLISLGLGLAVSACGSSSDEGDSGGSGKEGGEITISQTSQ
                     PDYLDPALSYTVNGWEPMWIVYTPLLTYPHVEGTKGGEVIPGLAEDLPKVSNGGKTYE
                     LKLRKGLKYSDGQPVVASDFTHTIKRVLNLESGGSFFFENIVGADEYIKNGDPEGDIK
                     GITTNDKTGEIKIDLISPDASFSNILATNFAGLVPGDTPFKNLTEDPPPGVGAYVITK
                     SVPNREFVMEKSPNFASFKIPQIPTGHVDKITTEIIKNANQQAQDVLANKLDYMQDPP
                     PADLKPTVEAEASDRYSETTTGSTYYFFMNTRVAPFNDPKVRQAVNYGIDKPGLARIF
                     AGELAPGCSFLPPGMPGYNEAFDTTDCPYGDPTQPPDLEKAKQLIKEAGVEGTKVTVW
                     GNNDDPTDKVTEAYADMLNQMGFDATPKILDGGVYFQTIGNQKTKAQTGFANWFQDFP
                     HPLNFSFLVDGDSIQDTNNQNFGNVDDPSLNKTINELTLETDLPSVADRWEAWDQELV
                     SPPNSYVAPYGHRKLATFVSDRIDFDAIVFHPLYNNDYSTFKLK"
     gene            complement(6384..6956)
                     /locus_tag="PLJ64_06510"
     CDS             complement(6384..6956)
                     /locus_tag="PLJ64_06510"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC93166.1"
                     /translation="MSSSSRPEILRATRTLIAKNGAQVSLAEVASEAGVSRQAVYMHF
                     GSRAGLLVALVRNMDEEAGIRQKLERALLREDPVEAFRQFVKGWLRYAAEIQPVASML
                     FAAREIDPAAAKAWEDRSRELRAGFRAATVRLGKAGMLRDGLSATSAASLSWAICSVP
                     VVEQLALDQEWSKDRVARQLTEAALSAITG"
     gene            6989..7396
                     /locus_tag="PLJ64_06515"
     CDS             6989..7396
                     /locus_tag="PLJ64_06515"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNC93167.1"
                     /translation="MSEHPALSAAREAMAAVSAGDRTAWLDLYDDDAVLEDPVGGSPL
                     DPEGVGLRGRTALEGFWDLVVGPNDFDFEITATHSGGSEAALSATVRGRYPNGAKTSY
                     DGIFIYAIGENGKITSVRAFWDIDAVMSALGAS"
     gene            7474..7848
                     /locus_tag="PLJ64_06520"
     CDS             7474..7848
                     /locus_tag="PLJ64_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HNC93168.1"
                     /translation="MGRDSELVWSSDGGDFRPEQKREAKKAKRRGGRSGAAPPPGQAP
                     AGSTIKVKRETSGRKGKTATTISEIPLGEAETKALAKKLKQLCGVGGTARGSSIELQG
                     DHRDKVIAYLEKEGHRAVKAGG"
     gene            complement(7845..11354)
                     /locus_tag="PLJ64_06525"
     CDS             complement(7845..11354)
                     /locus_tag="PLJ64_06525"
                     /inference="COORDINATES: protein motif:HMM:NF024220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich protein"
                     /protein_id="HNC93169.1"
                     /translation="MTFNYTGAPESWTVPYGVTSATFNLYGAQGRGFGPGSSAQGGRG
                     GWARATIPVTANSVVTFRVGGRGVANGGGYNGGGSGGYGGGGATDIRIGGDALADRVL
                     VAGGGGGGGFSCLNNNVTVIAVGGDGGGVNGWSGNNTGCSPSGSGGTSTHGGSPGGTL
                     GQGGGGGPSGGGGGYYGGGGSLPGYANGGPGGGGSSYGPAGFSTSSGLREGDGTATVT
                     FEVTDTRNLFASAVGPGDGFVSSSPAGIDCGVSEAPGHDDCTEAYGKDQPVTVTANPS
                     PGSVFDQWIGPGPCSGNTNPICSTTLNQVNPGDPKIVSARFKVPPVTRTFQYTGEAVQ
                     WTMPYGASSATIDLYGAQGGGPGGRFIGGKGGRATATIFPAGGTVFWLRVGGAGGAQT
                     GGFNGGGTSPSGPNAYGGGGATDLRTGNDTIADRILVAGGGGGGGQNCDDQSVVATGG
                     SGGGATGGPGDLNFAEGDPPAGCNIYRATGGTQTQKGSFQTGYPPVGGPQNEGGSGSN
                     GGGGGGGYYGGGGTNGPGGGGSSYGPFGYTTESGVREGNGLATITYTLTNTTGLEVGA
                     SGPGDGYVSSEPVGVDCGTTASHNTCYSIFGNGQPVTLTANPDSDSLFNSWGGDCSGT
                     QPTCTVTLDQTRSVTSSFSHGPRKLTVTRPGNGAGGVTSTPAGIDCGTTCEANFPYGT
                     AVTLTPGASTGSSFTGWTGACSGSGACSVTMDQARTVGATFTLQKRTLTLAKFENGSG
                     TVVSSPAGINFPTGATSCLVDGKCVNAQFDYGTEVTLTPTAATGSTFTGWGGACQSFA
                     IDQNCVVTMDQLNTAEAGFVLQKRTLEVAKTGSGTGGVTSATPGIDCGSDCSNDYDYG
                     TEVTLNPTPGPNSLFTGWSGACTGTAACVVTLDQARQVTASFALEKRSLGVTRSGNGS
                     GTVMSQPAGVACGGQCEGIEFDYGTEVTLSANPATSSDFSGWTGDCSGSGSTCIVTLD
                     QARSVEATFTLQKRTLTVETSGTGSGTVGTDPAGPDFDFGTPVTLEAVPANGSTFTGW
                     GGDCSGIVPTCTVDMSDARSVEARFTADPLPDPAPVIGQLRVAPRKVPLKGKKKSAIR
                     RQGPAIRIRLSEASTVRFSLKRKPGKARNFRRKLKAGTSKVRIPAGVRKLLRPGRFQL
                     TAVATDNAGQSSNPRRTGFKVVR"
BASE COUNT         1923 a         3709 c         3703 g         2050 t
ORIGIN      
        1 ctaaccctgc ttgaggtagt tgaccttctc gggttcgagg gtgaaagtga cccggacctc
       61 gcccggctgg cggaacggat actcgtcggc gtcgatgtac ttcttggcca gcatgttgat
      121 gtcgtcgtcg gcgccttcct gggtatcacc gatcagccgg ccgcggatcg acacgtactc
      181 gtaggggttg ccgtcggcca tcatcgtcac ggtcgcccgg cccgccttgc gcaggttctg
      241 cggccaggcc cgaccctcgg cactgttcag gatcacgttg ccgtcgtcgt cggcgtgcgc
      301 ccagacgatc accgactgca ccgagccgtc atcggtcggc acggcgacga ccgcatagtt
      361 cgcaccttcc ttgacgattt cgagcgaacg accttcgagt tctgccactt cgacctcctg
      421 ggttgtttga agacaggacc gaatctactc cccggccggg tccggtcgac cgaggggatg
      481 gtggcaggcg gcgaaatgac cctggccgta gtcgatcagg gccggctcct gttgctcgca
      541 gacttcggtt gcgtaggggc agcgggtgtg gaaacggcaa cccgagggcg ggtccgaggg
      601 gctcggaacg tcaccctcga gcggccggcg ctccctctgt gcgttggttc tcgggtcggg
      661 aatcgggacg gccgccagca gcgaggccgt gtagggatgg atcggtttcg agtagacgag
      721 ttcggccggg ccgatctcga cgatcttgcc cagatacatc acggcgatcc ggtcggacac
      781 ctgccggacc acgcccaggt catgcgccac gaagaggtag gtcagctcca gctcgcgctg
      841 gaggtcggcc atcagattca cgatctgccc ctgaatcgaa acgtcgagcg ccgagaccgg
      901 ttcgtcggcg acgatcaggc tcggttcggg cgcaagggca cgggcaattc cgatgcgctg
      961 acgctggcca cccgagaact cgtgcgggta gcggtcgtag tagctgccgg agagcccgac
     1021 ctggtccagc ttctcccgca cccggcgctc gatctcacgg ccctcggcca gaccgtggat
     1081 acgaagcggc tcaccgatga tctgcccgac ccgcttgcgc ggattcagcg aggtgtaggg
     1141 gtcctggaag atcatctgca tgtcccgccg gatcggccgg agtttccggt tgctgatttc
     1201 cgtcagctcg accccgcgga acctgactga accggagctt ggcctgagca actgcagcag
     1261 cgacctcgcc agggtcgact tcccgcagcc cgactccccg accagaccga gcgtttcacc
     1321 cttcttcagt tcgaagctga ccccgtccac cgcccggacc gcacccttgc cgcggccgag
     1381 cagtgaattt cccgagagcg ggaaatgctt gttcaggtcc ttgacctgca ccagggcctc
     1441 gcccgttgag gtcgattcca gctgggtggc ctgatcattc attcggggct ggccagtccg
     1501 atcagcccgt tcggcagttg ccgcagggcg ggtcgttcgg aaacctcgag ccagcagcgg
     1561 tcgaggtggc tcgggtccgc cacccggctg accaggtccg gttccacccc gcacttgtcg
     1621 aaggcgtggg ggcagcggtc acggaagcgg caaccggtcg gcggatcgat cagggaagga
     1681 ggctggcctt cgatctgggc gagcctcgcc tgccgggtgc tctcgatccg ggggaccgaa
     1741 ccgagcagcc cccaggtgta agggtgctgg gggtcgtaga agatctcgtc gagagtgccg
     1801 gtttcaacca gccggccggc gtacatgacc ccgacccggt cggcgatgtc ggcgacgacg
     1861 ccgaggtcgt gcgtgatcag gatcaccgtc aggttgaaat cccggttgag ctgcttgagc
     1921 agttccagga tctgggcctg gattgtgacg tcgagcgcag tggtcggttc gtcggcgatc
     1981 aggatctccg gttccagcga aagcgccatc gcgatcatcg ccctttgccg catgccgccc
     2041 gagaactcgt gcgggtaggc ccgggcccgg gcggctgcgt ccggcacacc gaccgcgtcg
     2101 agcagctcga tcgccctggc catcgcttcc ttcttgctga cgtcgcgatg ggcccggatc
     2161 tgttcgacga tctggttgcc gatccggtgg accggattca gggaggtcat cgggttctgg
     2221 aagaccattg acatgcggtc gccccggatc ggccgcatca cttcgttgct ggcaccgagc
     2281 agttcctggc cgtcgagctt gaccgagccc ttgaactcgg tgttgggacc gctggtcagg
     2341 cccataaggg tcatcgcggt taccgacttg ccgcagcccg actcgccgac gatcgagagc
     2401 gtctcgccgc gctggagatc gaacgaaacg cccccgacgg cctggagcaa cccgccctcg
     2461 gtgcggaagc tgacctcgag gtcgcgcacg ctgagcagcg gttcactcac agggctgtgt
     2521 ccccgtcctg cagacggata cgcgggtcca gtgccgcgta gacgatgtcg atcacggcgt
     2581 tgaagaagac cacgaagaag gcgccgaaca tggtcaccgc catgatcggc ggctggtcta
     2641 gctgcccgat cgcgtcggct gcatactggc cgacgccctg gaggttgaag acggtttcgg
     2701 tcaggatcgc gccgccaccg accaccaccg cgaagtcaag tccccagagg gttacgaccg
     2761 agatcatcga gttgcggagg acgtggctga gcatcaccct tcgctccgac agccccttcg
     2821 cccgggcggt ccggacgtag tcctcatcca tcgtttcgag gatgttcgcc cgaaggacgc
     2881 gggagtagaa gccgatgaac aggaccgaga gcgcgatcca gggcaggatc agatggtgaa
     2941 accactccca ggggctttgc gagaactcga cgtacccccc attcggaaat aactcgaagc
     3001 cgagtttctt ggtcaactcg aacccaaggt attggttcat cagcgctccg agccagaaca
     3061 ccggcatcga gattccgacc agcgcgaaga agttgagcag cccgtccgat attccgccgg
     3121 cccgcatcgc tccccacacc ccgagcgcga ccgcgaaggc catccagatg atcgcggcgc
     3181 cgatcgcgag ggcgaaggtc ctcgggatgc ccttcttgat ctcctccagc acgttgagct
     3241 ggttgaagta gctgatcatg ttgccgctga acaggttttc catggtcttc acgtactgga
     3301 cgtagaccgg ttcgttgaag ccccactcct ccctgatcgc ctcgatctgg gtttcggtcg
     3361 gctgcttgcc ggccatgcgg accgcggggt cgccgctggg caggacgttg aagatcagga
     3421 aggtcaggac ggacaccgcg aacagcacca ggaccatcga gcccagccgc ctcagagcga
     3481 acgccagcat cagtgttcga tcctcacttt cgccttcggg tcaagcgagt cgcggatcgc
     3541 ttcgccgaag acgttgagcg aaagcacggt gagcacgatc atcatgccgg gcacgatcgc
     3601 cagggcgggc gcggtgacga ttcgcgcgac accctcgtcg atcatggtcc cccaggaggg
     3661 ctcgggtggc tggacaccgg ctccgagaaa ggagagcgcg gcttcgagca ggatcgcgtt
     3721 ggcgaccagg agcggaaaga agacgagccc ggtcgaaagc acgttgggca ggatttcgga
     3781 ggtgatcagg cggaaggagc ccatgccctg ggcccgggcc gcctcgacga actccttctc
     3841 ccgcaggctc agcacctcgc cccggatcgg ccgggccagg taggggatgt agacgatcgc
     3901 gatcacgaag atcggaatcg cgaggctgcc gcccgacacc gacaccggac cgagcttgag
     3961 ccctccgaga gccagcgagg tgcccagggc gatccccagc aggatcaccg ggaaagccca
     4021 gaggacgtcc atgatcctcg aaaggatcga atcggtccag ccccggaaat atccggccag
     4081 gaggccggtc accagaccga ggaccagggt gatcagggcc gctccgaggc cgatgatcag
     4141 cgagttccgg ccaccgtaga gcagccgtac ggccacgtcg cgcccgttgg tgtcggcgcc
     4201 gaggaaaaac tggccttgcc aggtcggccc gatcggcacc ccgtccagcc cgaccacgtt
     4261 taccttctcg ccatcgactt cgatctggtc ggagagatgg ttcgcggagg gcgtggtgtg
     4321 agccacgtgc tcggcccaga gcggagcggc cgccatggtc aggacgagca ggacgaagac
     4381 cgctcccatg aagagagcga accgccgttt ccgcatccgc ctgaccgcca gccgccacgg
     4441 actctcgccg gccaccccct cctgggtgac ggccggcggt tccgagaatt cctggaggtc
     4501 agatacgggc ctgtccgtgg cgccgctcac tcttggtcaa tgactgcttt cgagggcctc
     4561 gggaatagct acttgagctt gaacgtggag tagtcgttgt tgtagagcgg atggaagacg
     4621 atcgcgtcga aatcgatccg gtcggaaacg aaggtcgcca gtttgcgatg tccgtacgga
     4681 gcgacatagc tgttcggcgg cgagacgagc tcctggtccc aggcctccca gcggtcggcg
     4741 accgagggca ggtcggtttc cagggtcagc tcgttgatcg tcttgttcag gctcggatca
     4801 tcgacgttgc cgaagttctg gttgttggtg tcctgaatcg aatccccgtc caccaggaag
     4861 gagaagttga gcggatgcgg gaaatcctgg aaccagttgg cgaagcccgt ctgggctttc
     4921 gtcttctggt tcccgatcgt ctggaagtag acgccgccgt ccaggatctt cggggtcgcg
     4981 tcgaagccca tctggttgag catgtcggcg taggcctcgg tcaccttgtc ggtggggtcg
     5041 tcgttgttgc cccagacggt taccttcgtg ccttcgacac cggcctcctt gatcagctgc
     5101 ttcgccttct cgaggtccgg cggctgtgtc ggatcaccgt aggggcagtc ggtggtatcg
     5161 aatgcctcgt tgtaacccgg catgccgggc ggcaggaagc tgcagccggg ggccagctca
     5221 ccggcgaaga tgcgggccag gccgggcttg tcgatgccgt agttgaccgc ctgacggacc
     5281 ttcggatcgt tgaacggagc cacccgcgtg ttcatgaaga agtagtaggt ggaaccggtg
     5341 gtcgtctccg agtaacggtc tgatgcctcc gcctccacgg tcggcttcag gtcggccggc
     5401 ggcgggtcct gcatgtagtc gagcttgttg gccagcacgt cctgcgcctg ctggttggcg
     5461 ttcttgatga tctcggtcgt gatcttgtcg acgtgaccgg tcgggatctg cggaatcttg
     5521 aagctggcga aattcgggct cttctccatc acgaattcgc ggttcggcac cgacttcgtg
     5581 atcacgtacg caccgacacc gggcggcgga tcctcggtca ggttcttgaa gggtgtgtca
     5641 cctggcacga ggccggcgaa gttggtcgcg agaatgttcg agaacgacgc gtccgggctg
     5701 atcaggtcga tcttgatttc gccggtcttg tcgttggtcg tgatgccctt gatgtccccc
     5761 tcggggtcac cgttcttgat gtattcgtcg gcaccgacga tgttctcgaa gaagaatgaa
     5821 ccgcccgatt cgagattcag gacgcgcttg atcgtgtggg tgaagtcgct ggcaacgacc
     5881 ggctggccgt cggagtactt gagtcccttg cgcagcttga gctcgtaagt cttgccgccg
     5941 ttgctgacct tgggcaggtc ctcggcgagt ccgggaatga cctcgccgcc cttggtgcct
     6001 tcgacgtgcg ggtaagtgag aagcggggtg tagacgatcc acatcggttc ccagccgttg
     6061 accgtgtagg aaagcgccgg gtccaggtag tcgggctgtg aggtctggga gatcgtgatc
     6121 tctccaccct ccttgccgct tcctcccgag tcgccttcgt ccgagctgga cccacaggcg
     6181 gacacagcca acccgagccc cagactgatc agaacgacag cgagcaactt cccaatcaac
     6241 gaatcccgca aatcacgaac caggttcatg gacgaaacgc ctctcctcgt tgtacagacc
     6301 ttgagcacat tggctcctct ctcccccgtc aggctagcgc caaaaccggc gcttcgggag
     6361 aaatccgtct cacccgcctc gattcaaccg gtgatcgcgc tcagcgcggc ttcggtcaac
     6421 tgccttgcga cccggtcctt tgaccattcc tgatcgagtg ccagctgctc gaccaccggc
     6481 accgaacaaa tcgcccagga caggctcgcg gccgaagtcg ccgaaagccc gtcccgcagc
     6541 attcccgcct tccccagtcg caccgtcgcc gcacggaacc ccgcgcgaag ttcccgggaa
     6601 cggtcctccc aggcctttgc cgcggccggg tcgatctccc tcgcggcgaa aagcatcgag
     6661 gcgaccggct ggatctccgc cgcgtacctc agccacccct tcacgaactg gcggaatgcc
     6721 tcgaccgggt cctcccgcag cagcgcccgt tccagctttt gcctgattcc ggcctcctcg
     6781 tccatgttgc gaaccagggc aacgagcaga cccgcacggg acccgaagtg catgtacacc
     6841 gcctggcggg aaactccggc ttcactggca acctcggcga gcgagacctg ggcgccattc
     6901 ttcgcgatca gggtcctggt ggccctgagg atctccggtc gggacgaact tgacatgtga
     6961 caagtttacg gtaactttac acgtgtaaat gtctgaacac ccagcacttt ccgctgcccg
     7021 cgaggccatg gccgccgttt ccgccggaga ccgcactgcc tggctcgacc tctacgacga
     7081 tgatgcggtt ctcgaagatc cggtcggcgg gtctccgctg gacccggagg gggttggcct
     7141 gcgaggccga accgccctcg aagggttctg ggacctcgtg gtcggtccga acgacttcga
     7201 tttcgagatc accgcgacgc attccggagg ctccgaggca gctctctcgg ccaccgtccg
     7261 cggcaggtac cccaacggcg caaagaccag ctatgacggc atcttcatct acgcgatcgg
     7321 tgaaaacgga aagatcactt cggtccgcgc cttctgggac atcgacgccg tgatgagcgc
     7381 gctcggcgct tcctgaccat caacgggtga gctcctggcc ttcaggagga acccgaggtc
     7441 gtccggaagc agaccggtgg gtagggtttc gccatgggtc gtgactcgga actcgtctgg
     7501 tcaagcgacg gcggggattt ccgtccggaa cagaagcggg aagccaaaaa ggcgaaaagg
     7561 cgcggaggca gatccggcgc ggctccaccg cccggccagg cgccggccgg ctcgacgatc
     7621 aaggtgaagc gggaaacctc gggccggaag gggaagaccg ccacgacgat ctcggagatc
     7681 cccctgggcg aagccgagac caaagccctg gcgaagaagc tgaagcagct ttgcggggtc
     7741 ggcgggaccg cgaggggttc cagcatcgaa ctccagggcg accaccgcga caaggtgatc
     7801 gcctacctcg aaaaggaagg tcaccgggcc gtaaaggccg ggggctagcg gacgactttg
     7861 aatccggttc ggcgtgggtt cgagctctga ccggcgttgt cggtcgctac ggcggtgagc
     7921 tggaaccttc ccggcctgag gagcttgcgg accccggccg ggatccggac cttgctggtg
     7981 ccggccttga gcttgcgcct gaagttcctg gccttgcccg gcttgcgttt gaggctgaaa
     8041 cgaacggtcg aggcttcgct tagcctgatc ctgatcgccg gtccctggcg ccggatcgcc
     8101 gacttctttt tgcccttgag cgggaccttt cgcggcgcga ccctgagctg accgatcacc
     8161 ggtgcggggt ccggcaacgg gtcggcggtg aacctggcct cgactgaccg ggcgtctgac
     8221 atatcgaccg tgcaggtcgg cacgattccg gaacagtccc cgccccaccc ggtgaaagtc
     8281 gagccgttcg cgggaaccgc ttccagggtg accggagttc cgaagtcgaa atcggggccg
     8341 gccgggtctg taccgactgt gcccgacccg gtgcctgagg tctcaaccgt cagcgtccgc
     8401 ttctgaaggg tgaacgtggc ctcgacggaa cgggcctggt cgagggtgac gatgcaggtg
     8461 gaacccgatc ccgagcagtc cccggtccat ccgctgaaat cggaactcgt ggccgggttc
     8521 gccgagaggg tcacttcggt gccgtaatcg aactcgatgc cttcgcattg gccaccacag
     8581 gcgactcccg ccggctgcga catgaccgtg cccgaaccgt tgccggaccg ggtcacgccc
     8641 aggctgcgct tctcgagcgc gaaggaggcc gtgacctgac gggcctgatc cagggtgacc
     8701 acgcaagccg cggttccggt gcaggccccg ctccagccgg tgaacagtga gttcggcccg
     8761 ggagtggggt tcaaggtgac ctcggtgccg tagtcgtagt cgttcgaaca gtcgcttccg
     8821 caatcgatgc cgggagtggc cgaagtcact ccgcccgtcc ccgacccggt cttcgcgact
     8881 tccagggtcc gtttctgcag cacgaagccg gcttcggcgg tgttgagctg gtccatggtc
     8941 acgacacagt tctggtcgat cgcaaatgac tggcaggctc cgccccagcc ggtgaaggtc
     9001 gacccggtcg ccgcggtcgg ggtcagcgtt acctcggtgc cgtagtcgaa ctgggcgttc
     9061 acgcatttcc cgtcgacgag gcaggatgtc gctccggtcg ggaagttgat gcccgccggg
     9121 ctcgagacga cggttcccga tccgttctcg aacttcgcca gggtcagggt gcgcttctga
     9181 agggtgaatg tcgctccgac ggtccgggcc tggtccatcg tcaccgagca cgcgcccgag
     9241 ccggaacagg cgccggtcca gccggtgaag ctggaaccgg tcgaggcacc cggcgtcagc
     9301 gtgaccgcgg tgccgtaggg gaagttcgcc tcgcaggtgg ttccgcaatc aatgccggcc
     9361 ggggttgagg tgacgcctcc cgcaccgttg cccggcctgg tgaccgtgag cttccgcggt
     9421 ccgtgagaga aggatgaggt gacgctccgg gtctgatcca gagtgacggt gcaggtcggc
     9481 tgggtgcccg agcagtcccc gccccaactg ttgaagagcg agtcggaatc ggggttggcg
     9541 gtgagggtca ccggctggcc gtttccgaag atcgagtagc aggtgttgtg ggaagcggtg
     9601 gtgccgcagt caacgccgac cggctccgaa ctcacgtagc cgtcacccgg tccgcttgcc
     9661 ccgacctcga gacccgttgt gttggtcaag gtgtaggtga tggtggccag cccgttgcct
     9721 tccctgaccc cggattcggt tgtgtagccg aacggaccgt agctcgaccc gccaccgccg
     9781 ggcccgttgg tgccaccacc accgtagtag ccgccgcctc ctccgcctcc gttggagccg
     9841 ctgccgcctt cgttctgtgg gcccccgacg ggcgggtacc cggtctggaa ggaacccttc
     9901 tgggtctggg tgccaccggt tgccctgtag atgttgcaac cggccggagg atcgccttcg
     9961 gcgaagttca ggtcacccgg acctcccgtg gcgccgcctc cgctgcctcc ggtagcgacg
    10021 acgctctggt catcgcagtt ctggccgccg cctccaccac caccggcgac gaggatccgg
    10081 tccgcgatgg tgtcgttccc ggtcctgaga tcagttgctc cgcctccacc gtaggcgttg
    10141 gggccagacg ggctcgttcc accgccgttg aaacctccgg tttgcgctcc cccggcaccc
    10201 cctacccgaa gccagaagac cgtccctccg gccgggaaga tcgtcgccgt ggcgcggcca
    10261 cccttgccgc cgatgaatcg tccgcccgga cctccaccct gggccccgta caggtcgatc
    10321 gtcgccgacg aagccccgta gggcatcgtc cactgcaccg cttcgccggt gtactggaag
    10381 gtccgggtta cgggcggaac cttgaacctg gccgaaacga tcttgggatc gccgggattg
    10441 acctggttga gggtcgttga acagattgga ttggtattac ccgaacaagg ccctggaccg
    10501 atccactggt caaagaccga ccccggggaa gggttcgcag tgaccgtaac cggctgatcc
    10561 ttgccgtagg cctcggtgca gtcgtcgtgt ccgggcgcct cgcttacccc gcaatcgatc
    10621 ccggcgggag agctggacac aaaaccgtca cccggtccga ccgcggaggc gaacaggttg
    10681 cgggtatcgg ttacctcaaa ggtgaccgtg gcagtcccgt cgccttccct caagccggaa
    10741 ctagtcgaaa agccggccgg gccgtagctg gatccaccac caccggggcc accgtttgca
    10801 taccccggca gggatccgcc gccgccgtag taaccgccgc cgcctccgct tgggccgccg
    10861 ccgcctccct gaccgagggt gcctccggga ctgccgccgt gcgtgctggt cccgccgctt
    10921 ccggacggtg aacaaccggt gttgttcccg gaccagccgt tcacaccacc gccgtcgccg
    10981 cccacggcga ttacggtgac gttgttgttc aggcagctga agcctccgcc cccgcctccg
    11041 ccggccacga gtacacggtc ggccaaggcg tctccgccaa tgcgtatgtc ggtggccccg
    11101 ccgccaccgt agccaccgct gcctcccccg ttgtatccac cgccgttggc tacgccccgg
    11161 ccacccaccc ggaaggtcac caccgagttt gcagtcacgg gtatcgtggc cctggcccag
    11221 ccgcctcgac ctccctgagc agatgatccg ggtccgaaac ctctcccctg ggctccgtac
    11281 agattgaagg tcgccgaggt gacgccgtaa ggaaccgtcc aggactccgg ggcaccggtg
    11341 tagttgaagg tcacggtcgt cgcccctgcc gacgacggca gcacg
//
LOCUS       DAOQKN010000074        11270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_2702_length_11270_cov_5.475078, whole genome shotgun sequence.
ACCESSION   DAOQKN010000074 DAOQKN010000000
VERSION     DAOQKN010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 11270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11270
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2702_length_11270_cov_5.475078"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..1700
                     /locus_tag="PLJ64_06530"
     CDS             108..1700
                     /locus_tag="PLJ64_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNC93170.1"
                     /translation="MAASPPPGTSGRFLGLLVFTTGACTMGAEIAGARLLAPYFGDST
                     IVWANTIAIVLVALSIGYWIGGRLADRHPDLRSLCGTVLCGAILVTLIPFFARPFLGA
                     SVDAFDELSVGVFAGSLAGVLVLLAIPVMIFGAVSPWALRIAMDNSDVEHAGQLAGRL
                     YALSTAGSLVGTLVSAMVTIPLIGTQRTFIVFALLVAIVASIGLRKLWPALIPAAVAC
                     AALVPVGTIKASSDGEVIFEAETPHQYIRVVETDDGERRLELNEGQAIHSIYRPGEYL
                     VGNYWDEHLVLPFVAPPGVTGARQATWATLSAHPKPLKPWPRRIAILGNAAGTVARAY
                     GHFYPGTKIDAVEIDGKVTEVGRKYFGLDNPNMEVHTEDARPWLEAADPVYDVIMVDA
                     YHQPYIPFYMATKEFFELVRDRLRPGGVVLINVGHPENSDALEENLTATLEEALPEVL
                     RDPVTDTNTMLVGAAGPIEPGNIIQATKGKSSPIPPALDNLATDTAIRIRPPLSGGDI
                     WTDDHAPVEWLVDLSLLDYARE"
     gene            1750..2034
                     /locus_tag="PLJ64_06535"
     CDS             1750..2034
                     /locus_tag="PLJ64_06535"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HNC93171.1"
                     /translation="MPKVGVRELRQNLSRHLQRVKAGESLEVTEHGRVVARLSPAGEN
                     LPEAYLALAADHGAQVPSTSLSDLVANRPRRATPAGTVDAMLAEGRAERS"
     gene            2040..2441
                     /locus_tag="PLJ64_06540"
     CDS             2040..2441
                     /locus_tag="PLJ64_06540"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNC93172.1"
                     /translation="MAGVYLDTSALGHLVLDEPDADVIGSALTRFEVVVSSRLLRTEL
                     HRLGLRTGVPSDEIASWLTSLALVPVDEEILSAAESIGPASVATLDAIHLVTAIRLAA
                     SGHISSVMTFDVRLAEGAAQHQLEVIAPVGE"
     gene            2443..2565
                     /locus_tag="PLJ64_06545"
     CDS             2443..2565
                     /locus_tag="PLJ64_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93173.1"
                     /translation="MKRAPIRAWLVTGPPGRLVGFLLDFARALRAHRRAKKAER"
     gene            2562..3134
                     /locus_tag="PLJ64_06550"
     CDS             2562..3134
                     /locus_tag="PLJ64_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HNC93174.1"
                     /translation="MSDEYLLIHFEDGEQLGAWLEENGETHDEVWVEIYKKHTGVKSI
                     AWAEIVDTVLCFGWIDSQSRRIDEDRYRQRLTPRRKRSKWSKINCDKAEALIADGRMR
                     PAGLAEVERAKEDGRWEEAYDSPNTATVPEEFEKALEAAGLTEKFSKLKSQERYSMLH
                     RIQTAKRADTKARLIEKFCGMISRGEQVHP"
     gene            3189..4565
                     /gene="tig"
                     /locus_tag="PLJ64_06555"
     CDS             3189..4565
                     /gene="tig"
                     /locus_tag="PLJ64_06555"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNC93175.1"
                     /translation="MKTTVTELEDSRARVEVEVPADEVSVQVQRAARALAREMRMPGF
                     RKGKAPPSLAIQRLGYDTVFQEAIQEGLPEWYQKSVYASGVVPIGSPDVEITKAPTQE
                     GETVEFKYEVGVRPNAELGEYKGLEVEKAGTEVPDEVIDREIDRMREGMASLDVVDRA
                     AEEGDHVLVDFVGSLDGVEFEGGSANDHTIEIGSGQLIDDFEEQIIGAKAGDEVAVNV
                     NFPEEYGAAELAGQNADFKVTVKEVRVKQMPEADDDFASEASEFDTIEELRADIAEKL
                     GESAGQRIEQDFRVAAVDAAVKNAKVGIPEDILRARGEERWERVERQMAQQGMNPDTY
                     LQMQGKTRDEIIDESLDDADMEIRREAVLVAVADAEGIEVTDEEMAEELEHAASHERT
                     TGAKLLERLKRDGRHEMVIADIKVRKAMDVIADSATATEMSSEETVKKLWGLGDDEED
                     SETEPSSE"
     gene            4686..5270
                     /gene="clpP"
                     /locus_tag="PLJ64_06560"
     CDS             4686..5270
                     /gene="clpP"
                     /locus_tag="PLJ64_06560"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HNC93176.1"
                     /translation="MSPLVPMVVEQTSRGERSFDIYSRLLNERIIFLGTGVNEDIANL
                     VVAQLLHLDAEDPEKDISLYINSPGGSVYAGLAIYDTMQFIKPDVQTICVGIAMSMGA
                     LLLAGGAAGKRMSLPNSKILIHQVSSGFQGQATDIEIHANEIIDTRSRLDKILAKHTG
                     QDLEKITHDTERDYFMSAEEATTYGLIDRVIEHR"
     gene            5427..6119
                     /locus_tag="PLJ64_06565"
     CDS             5427..6119
                     /locus_tag="PLJ64_06565"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNC93177.1"
                     /translation="MTPKEAIPFSVDPIRIHLRFSPKGARHISIRVRKLGSGRTIRRI
                     VTGRLAGARWHHIYWDGLTQRRRLAPAGRYLVVAGPIGGPLKRVGRIRLHSHEFPVDG
                     PHGTRGYIGSFGAPRVDGRTHEGFDITAACGTPLVAVRAGRIIKKAYDPELYGNFIVL
                     KGKGERRTYKYAHLIRPAPVRRGQRVRAGRRLGSVGQTGNAAGTPCHLHLEIRTHGHL
                     LNPEPLVNAWDW"
     gene            complement(6372..7394)
                     /locus_tag="PLJ64_06570"
     CDS             complement(6372..7394)
                     /locus_tag="PLJ64_06570"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde reductase"
                     /protein_id="HNC93178.1"
                     /translation="MTATDGAKGTVLVTGGSGFLGGRAIIDLLERGYDVRTTVRSKSK
                     APRIEENISNEIGHPAGIEFFEADLASDDGWAEAVSGCRYVLHIASPFPPNKPKDPQE
                     LIKPAREGAVRVINAALDAGVERIVMTSSVAAIRGGENPEDRFYNEDDWTDVDAVGAY
                     PQSKTLAEQAAWDLVDQRGERQKLATICPSAILGPVMPDDDSYSLEGIQRLLDGMPAV
                     PEIGFSWVDVRDVSAAHIAAMEKPEAGGQRFIASGPFLWLLEVSEILREKLPELAKKA
                     PTRKAPKFMVRIMAIFDPGARPLIADIGQRNDFDTTRAKEVLGIEARPLEETFVECAE
                     SLAARG"
     gene            7418..8179
                     /locus_tag="PLJ64_06575"
     CDS             7418..8179
                     /locus_tag="PLJ64_06575"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HNC93179.1"
                     /translation="MSRLIDLSAPIEPGPEGAPEVVRIDVEYTPHEEGAKQIQALFGV
                     GPELLRDGEGWAVDTFTLLGTHSSTHVDAPWHYNSTIQGKPAETIDQLPLDWFFRPGF
                     LLDFHERADGEVVEIADIEAELQRTGHEIADRDICLVRTGCDRYLGTLEYMAHGPGVS
                     AAATRWLYDRGVRVMGIDAWGWDRPLWMQAEEAKEMGEAGIFWEAHQADIPYSQIERL
                     ANLDQLPATGFTVACFPLKLVGGSAAPARVVAIID"
     gene            complement(8169..9581)
                     /locus_tag="PLJ64_06580"
     CDS             complement(8169..9581)
                     /locus_tag="PLJ64_06580"
                     /inference="COORDINATES: protein motif:HMM:NF013155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93180.1"
                     /translation="MKSRPFFRLLILGLLALALVPASAGASVAGAAKRLDNLRDQPQR
                     LRQFLLGMPKGTDLHTHLSGAVYAEAMIRWGAEDGKCVDPVTFVSTFPPCTGSQVPLT
                     AALTNTQLWDQILAAWSMREFRPDAFSGHNHFFATFDLFGSAFSGRQGDGLAQVTQRA
                     ASQNVQYVEPLITPQFGATLAIANQVGYIPDFAEMREAMLNAGITNAIPAAGLTASNA
                     LGREFDLLGCATGDPQPGCGVLVHFDVQVLRNQLPAVVFAQLLFGMELMKADPNWAGM
                     NMVQPEDAAYSTSDYNLHMRMVRYLRSVYPDESVTLHAGELVPGIVPPDDLRFHIRQA
                     VNIAGADRIGHGADLRWERNPDQILRSMKSKGTCLEANLTSNQQILRLSGKRHPITDY
                     VRAGVKVTLSTDDEGVSRTDLTAQYVKAVRQHGFGYRRLKQFATDGLRCAFLTAAEKK
                     AALADQKQRFRKFEARFPSR"
     gene            complement(9607..11025)
                     /locus_tag="PLJ64_06585"
     CDS             complement(9607..11025)
                     /locus_tag="PLJ64_06585"
                     /inference="COORDINATES: protein motif:HMM:NF021523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HNC93181.1"
                     /translation="MTSASGTSTLTQDSPRFTGKELRRKASRESHADWEPAPGRDPLK
                     ILLAQGESRVQDLLPIRYGRMSASPFAFYRGGAAIMAADLAHTPATGVRVQACGDAHI
                     SNFGGYAAPDRKLVFDLNDFDETLPAPWEWDVKRMAASAAIAARENGASKKVARRIVL
                     AGMANYRRVMRELAEKSYLDVWYERLDVERMIEFLEEENGTDWGVSLRRDTRKASRKD
                     SSRAQRKLTEGSAEGEPRFASRPPLLVPASELAASLGVNDIDMIWGYLEQLMGQYADT
                     LAADRKYLFEHYEFVDMARKVVGVGSVGTRAWVLLFISRRRKDPLVLQVKEAQASVLA
                     PYAGASKYRLQGRRVVEGQRLTQAASDLFLGWIRAKGPDGNERDFYIRQLWDGKLSID
                     IGSLDELGLTEYVRACGGTLALAHARTGDRVGIADYLGKNDSFDQAIADFSLSYAKQN
                     QEDFKAFTAEIEAGRIDATPGI"
BASE COUNT         1981 a         3677 c         3752 g         1860 t
ORIGIN      
        1 gaaagccggc cacggagacc tgctcgagaa gcggatccgg gccggcggga acggcgcggc
       61 ggcaggcgaa gaggcgatgc tggtcgggga cccgggtccg aatgccgatg gccgctagcc
      121 cgccgccggg gacttccggc cggttcctcg gcctgctcgt cttcaccacc ggcgcctgca
      181 cgatgggtgc cgagatcgcc ggagcccggc tgctcgcgcc ctacttcggt gattcgacca
      241 tcgtctgggc gaacacgatc gcgatcgtcc tggtcgcact ttcgatcggc tactggatcg
      301 ggggcaggct ggccgaccgt cacccggatc tcaggtcgct ttgcgggacg gtgctctgcg
      361 gggcgattct cgtaaccctg atcccgttct tcgcccggcc gtttctcggc gcctcggtcg
      421 atgcttttga cgaactctcg gtcggcgtct tcgccgggtc actggccggc gttctggtcc
      481 tgttggcaat tccggtgatg atcttcggtg cggtctcgcc ctgggcgctc cggatcgcta
      541 tggacaactc ggacgtcgag catgccggcc agctggcggg ccggctctac gcgctttcga
      601 ccgccggctc gctggtcggc accctggttt cggcgatggt caccatcccc ctgatcggca
      661 cccagcggac tttcatcgtc ttcgcgctgc tggtcgcgat cgtcgcatcg atcgggttga
      721 ggaagctctg gccggcgctg attccggccg cggtcgcctg cgccgcactg gtcccggtcg
      781 gaacgatcaa ggcctccagc gacggggagg tcatcttcga agcggagaca ccccatcagt
      841 acattcgggt ggtcgaaacc gatgacggcg agcggcggct cgaactgaac gaggggcagg
      901 cgatccactc gatctatcgg cccggcgagt acctggtcgg caactactgg gacgagcacc
      961 tggtcctgcc cttcgtggcg ccgccggggg tgaccggcgc ccggcaagca acgtgggcga
     1021 ctctgtcggc tcacccgaag ccgttaaagc catggccacg gcggatcgcc atcctcggca
     1081 acgccgccgg cacggtcgcc cgggcctacg gtcacttcta tcccgggacg aagatcgacg
     1141 cggtcgagat cgacggcaag gtgaccgaag tggggcggaa gtacttcggt ctcgacaatc
     1201 cgaacatgga ggttcacacc gaggatgccc gaccgtggct cgaggcggct gacccggtct
     1261 atgacgtgat catggtcgat gcctaccacc agccctacat tcccttctac atggcgacga
     1321 aggaattctt cgaactggtc cgggaccggc tccgcccggg cggggtggtg ctgatcaacg
     1381 tcggccatcc cgaaaacagc gacgccctgg aagagaacct gaccgccacc ctcgaggagg
     1441 ctctgcccga ggtcctccgc gatccggtca ctgacaccaa cacgatgctg gtcggcgcgg
     1501 ccggcccgat cgagccaggc aacatcatcc aggccaccaa gggcaagagc tcacctatcc
     1561 cgccggcgct cgacaacctg gccaccgata ccgcgattcg gatccgcccg cctctgagcg
     1621 ggggagacat ctggaccgat gaccacgccc cggtcgaatg gctggtcgac ctttccctgc
     1681 tggactacgc ccgcgagtga gaaggtcttt tgtggtggat tcagtcaatg tgctacggtg
     1741 tgacgcacaa tgccgaaagt aggagttcga gagctgagac agaacctgag tcgccacctt
     1801 cagcgcgtga aggcgggtga gtccctcgaa gtcaccgagc acgggcgcgt ggtagccagg
     1861 ctttccccgg cgggtgaaaa tttgcctgag gcttatctgg cactggccgc agaccatgga
     1921 gcccaggtgc caagtaccag tttgtcggac ctggtcgcga atcgcccaag gcgagcgacc
     1981 ccggctggca cggttgacgc aatgctggcc gagggtcgcg ccgagcgatc ctgaaaccga
     2041 tggcgggcgt ttatctggac accagcgcgc tcggccattt ggtgcttgac gagcccgatg
     2101 ccgatgtgat cggttcggct ttgacccggt ttgaggttgt cgtctcaagc cgtctccttc
     2161 gtaccgagct tcatcgcctc ggccttcgaa ctggcgtacc aagtgatgag atcgcatcgt
     2221 ggttgaccag cctcgccctg gtgccggtcg acgaagagat cctctcagcc gccgagtcga
     2281 tcgggcccgc gtctgtcgcg acgctcgacg ccatccacct cgtcaccgcg atccggctcg
     2341 cagcctcagg tcacatctcg tcggtgatga cattcgatgt tcgactcgcc gaaggcgcgg
     2401 cacaacatca actcgaagtc atcgccccgg tcggtgagtg agatgaagcg cgcccccatc
     2461 cgagcctggc tggtcaccgg cccacccggc cgcctggtcg gcttcctcct cgacttcgcc
     2521 agggccctgc gcgcccaccg tcgggcgaag aaggccgaac gatgagcgac gagtacctcc
     2581 tgatccactt cgaggacggc gaacagctgg gcgcctggct tgaagagaac ggtgagaccc
     2641 acgacgaggt ctgggtcgag atctacaaga agcacaccgg ggtgaagtcg atcgcctggg
     2701 ccgagatcgt ggacacggtg ctctgtttcg gctggatcga cagtcagagt cgccggatcg
     2761 acgaggaccg gtaccggcag cggctgaccc cgcgacgcaa acgcagcaag tggtcgaaga
     2821 tcaactgcga caaggcggaa gcgctgatcg ccgacgggag gatgcgtccg gccggtctgg
     2881 ccgaggtgga acgggcaaag gaagacggca ggtgggagga ggcctacgac agcccgaaca
     2941 ctgcaaccgt ccccgaggag ttcgagaagg cgctggaagc cgccggcctg accgagaagt
     3001 tctcgaagct gaaatcccag gagcgctact caatgctcca ccggatccag accgcgaaac
     3061 gggcagacac caaggcccgc ctgatcgaaa agttctgcgg catgatcagt cgcggcgagc
     3121 aagtccatcc ctaggcgcgg cggctctgga agcggcggaa agccggtccc cgataccctc
     3181 attcgacctt gaagaccact gttactgaac tagaagattc gagggcacga gtcgaggtcg
     3241 aagttccggc cgacgaagtg tccgtccagg tccagcgggc cgcccgggcg ctggcccgcg
     3301 agatgcgcat gcccggtttc cgcaagggca aggcgccgcc ctcgctggcg atccagcgac
     3361 tcggctacga caccgtcttc caggaggcga tccaggaggg cctgcccgag tggtaccaga
     3421 agagcgttta cgcgtcggga gtggttccga tcggctcgcc ggacgtcgag atcaccaagg
     3481 ctccgaccca ggaaggcgag acggtcgagt tcaagtacga ggtcggcgtt cgcccgaacg
     3541 ccgaactcgg tgaatacaag ggcctcgagg tcgagaaggc cggcaccgag gtgcctgacg
     3601 aggtcatcga ccgcgagatc gaccgcatgc gtgagggcat ggctagcctc gacgtggtcg
     3661 accgtgcggc cgaggagggc gaccacgtcc tcgtcgattt cgtcggctcg ctcgacgggg
     3721 tcgagttcga gggcggctcc gccaacgacc acacgatcga gatcggctcc ggccagctga
     3781 tcgacgactt cgaagagcag atcattggcg ccaaggccgg ggacgaggtc gccgtcaacg
     3841 tgaacttccc cgaggagtac ggggcggccg agctggccgg ccagaacgcc gacttcaagg
     3901 tgaccgtcaa ggaagtccgg gtgaagcaga tgcccgaggc cgatgacgac ttcgcctccg
     3961 aggcttccga gttcgacacg atcgaggagc tccgcgccga catcgccgag aagctgggtg
     4021 aatccgccgg gcagcggatc gagcaggact tccgggtcgc cgcggtcgac gccgcggtga
     4081 agaacgccaa ggtcgggatt cccgaggaca tcctccgggc ccggggcgaa gaacgctggg
     4141 agcgggttga gcggcagatg gcccagcagg gcatgaaccc cgacacctat ctccagatgc
     4201 agggcaagac ccgggacgag atcatcgacg agtcgctcga cgacgccgac atggagatcc
     4261 gccgcgaggc tgtcctggtc gcagtggcgg atgccgaagg gatcgaagtc accgacgagg
     4321 agatggccga ggaactcgag cacgccgcgt cccacgagcg caccaccggc gccaagctgc
     4381 tcgaacgact gaagcgcgac ggccgccacg aaatggtgat cgccgacatc aaggtccgca
     4441 aggccatgga tgtgatcgcc gattcagcga cggccaccga aatgtcctcc gaagagacgg
     4501 tcaaaaagct ctggggcctc ggggacgacg aagaggattc ggaaaccgaa ccctcttcgg
     4561 aataagcaag tcgggcgggg tccgacccgc cctccacgag aaacccgccg gcccacagag
     4621 acatttgacg gatgggccgg ctctggtgga gtcggcccga taactaccct ctataggatg
     4681 gcggcatgag tccacttgtt cccatggtcg tcgagcagac ctctcgcgga gagcgttcgt
     4741 tcgacatcta ttcccggctc ctcaacgagc gcatcatctt cctcggcaca ggcgtgaacg
     4801 aggacatcgc gaacctggtg gtggcccagc tgctccacct ggacgctgag gatcccgaga
     4861 aggacatcag cctctacatc aactcgcccg gcggctccgt ttacgcgggc ctcgcgatct
     4921 acgacacgat gcagttcatc aagcccgacg tccagacgat ctgcgtcggc atcgcgatga
     4981 gcatgggcgc cctgctgctc gccggtggcg ccgcgggcaa gcggatgtcg ctgcccaact
     5041 cgaagatcct gatccaccag gtctcctccg gtttccaggg tcaggccacg gacatcgaaa
     5101 tccacgccaa cgagatcatc gacacccgca gccggctcga caagatcctc gccaagcaca
     5161 ccggccagga cctcgagaag atcacccacg acaccgagcg cgactacttc atgagcgccg
     5221 aagaggccac cacctacggc ctgatcgacc gggtaatcga gcatcggtag aggcgcatcc
     5281 tgaaagggag cctcaagagt ttcaccggag ccgtcgccat cctggcggcg gctctggtct
     5341 ttgcagggca cgcatccgcc acgtcggaac tttcccacgc cggggcgggc gaagggggaa
     5401 gcggattccg gctcctggcc ggtgaggtga cgccgaaaga agcaatcccc ttctcggttg
     5461 acccgatccg gattcatctt cgcttcagcc cgaagggagc ccgtcacatt tcgatcaggg
     5521 tcaggaagct cgggtccggc cggacaatcc gtcgcatcgt caccggtcgg ctcgccggtg
     5581 cgcgctggca tcacatctat tgggacggcc tcacccagcg gcgccggctc gcccccgccg
     5641 gccgttacct ggtggttgcc gggccgatcg gtgggcccct gaagcgggtc gggcggatcc
     5701 gcctgcactc gcacgagttc ccggtcgacg ggccgcacgg cacccgcggc tacatcggca
     5761 gcttcggcgc accccgggtg gacggccgca cccacgaggg ctttgacatt accgccgcct
     5821 gcggtacccc gctggtcgcg gtgcgggcgg gcaggatcat caagaaggca tatgaccccg
     5881 agctctacgg caacttcatc gtgctgaagg gcaaggggga gaggcgcacc tacaagtacg
     5941 cccacctgat ccggccggcc ccggtccgcc gcggccagag ggtgagggcc ggccgccgcc
     6001 tcggctcagt cggccagacc ggaaacgccg ccggaacccc ctgccacctc cacctggaaa
     6061 tccgcaccca cggccacctt ctcaaccccg agcctctcgt gaacgcctgg gattggtaag
     6121 gacgaatgcc cactttgtgc cggtatgtgg cgcaaagtgg aaactcgcgg gtcctggagc
     6181 caagtagtcc gtggaggtgc cgtacaaaca gcaccaccaa ggtcctgaac gtgcggcacc
     6241 tctaatcacg aaccgggttc gtgtagccgt ggggccgggt gagcgtcgtc cgggacactc
     6301 cagggccagc gcggctgaat gccgcgcgtg gtccgtcccg gcgatgctca cccggccccg
     6361 cggcgtaacc actaaccccg ggcggcgagg gattcggcgc actcgacgaa ggtctcttcg
     6421 agcggccggg cctcgattcc cagcacctct ttggcccggg tggtgtcgaa gtcgttgcgc
     6481 tggccgatat cggcgatcag cgggcgggcg cccggatcga agatcgccat gatccggacc
     6541 atgaacttgg gggccttgcg cgtcggggcc ttcttcgcca gctcgggcag cttctcccgc
     6601 aggatctccg agacctcgag cagccagagg aacggcccgg aagcgatgaa acgctgcccg
     6661 ccggcttccg gcttctccat cgcggcgatg tgagcggcgg aaacgtcgcg cacgtcgacc
     6721 cagctgaagc cgatctccgg caccgccggc ataccatcga gcaggcgctg gattccctcg
     6781 agggaatacg aatcgtcatc gggcatgacc gggccgagga tcgcgctggg gcagatcgtc
     6841 gccagcttct ggcgctcgcc gcgctgatcg accaggtccc aggcggcttg ctcggccagc
     6901 gtcttcgact gcgggtaggc gccgaccgca tccacatcgg tccagtcgtc ctcgttgtag
     6961 aagcggtctt cggggttctc gccgccacgg atcgcggcaa ccgaggaagt catcacgatg
     7021 cgctcaaccc cggcgtcgag ggcggcattg atcacccgaa ccgcaccctc acgggcgggc
     7081 ttgatcagct cctgcggatc cttcggtttg ttgggcggga acggcgaggc gatgtggagc
     7141 acgtaccggc aaccggaaac cgcttcggcc cagccgtcgt cggaggcgag gtcagcctcg
     7201 aagaactcga tcccggccgg gtggccgatc tcattcgaga tgttctcctc gattcgcggc
     7261 gccttcgact tgctgcggac cgtggtccgc acgtcgtagc cccgctcgag cagatcgatg
     7321 attgcccggc caccgaggaa gccggagcct ccggtgacca ggaccgtccc cttcgcgcca
     7381 tcagttgcgg tcatgaccgc actctagtct gccttgcatg agcagattga tcgatctatc
     7441 ggctccgatc gagccgggac ccgaaggtgc ccccgaagtc gtccgcattg acgtcgaata
     7501 cacgccgcat gaggaaggtg cgaagcagat ccaggcgctg ttcggggtcg gccccgaact
     7561 gctccgcgac ggcgaaggct gggcggttga caccttcacc ctgctcggca cccacagctc
     7621 gacccacgtc gacgctccgt ggcactacaa ctcgacgatc cagggcaagc cggccgagac
     7681 gatcgaccag cttccgctcg actggttctt tcggcccggg ttcctgctgg acttccacga
     7741 gcgggccgac ggcgaggtgg tcgaaatcgc cgacatcgag gcagaacttc agcggaccgg
     7801 acatgaaatt gcggaccggg acatctgcct ggtccggacc ggctgtgacc ggtacctcgg
     7861 caccctcgaa tacatggcac acggccccgg tgtgagcgcg gccgccacgc gctggctcta
     7921 cgatcgcggc gtccgggtga tgggaatcga cgcctggggc tgggaccggc cgctctggat
     7981 gcaggccgaa gaggcgaagg agatggggga ggccgggatc ttctgggagg cccatcaggc
     8041 cgacattccc tattcccaaa tcgagcggct ggccaatctc gatcagctgc cggcgaccgg
     8101 tttcaccgtg gcctgcttcc cgctcaagct ggtcggcggc agcgccgccc ccgcccgggt
     8161 cgtggcgatc atcgactagg gaagcgcgct tcgaacttcc ggaagcgctg cttctgatcg
     8221 gccagggcgg ctttcttctc ggccgcggtg aggaaggcgc agcgcaggcc gtccgtcgcg
     8281 aactgcttca gccgccggta accgaagccg tgctgccgca ccgctttcac gtactgcgcg
     8341 gtcaggtcgg tgcgggacac gccttcgtcg tcggtcgaga gggtcacctt gactccggcc
     8401 cgcacataat cggtgatcgg gtggcgcttt ccggacagcc ggaggatctg ctggttcgag
     8461 gtcagattcg cttccaggca ggtccccttg ctcttcatgc tgcggaggat ctggtcgggg
     8521 ttacgctccc agcgcaggtc ggcgccgtgg ccgatccggt cggcaccggc gatgttcacc
     8581 gcctgcctga tgtggaagcg gaggtcgtcg ggtggcacga tgcccggcac cagctcgccg
     8641 gcgtggaggg tcacgctctc atccggatag accgacctca ggtacctgac catccgcatg
     8701 tggaggttgt aatccgaagt cgagtaggcg gcgtcctcgg gctggaccat gttcatgccg
     8761 gcccagttcg gatcggcctt catcagttcc atcccgaaga gaagctgggc aaacacaacc
     8821 gccggcagct ggttgcgcag gacctgcacg tcgaagtgga ccagcactcc gcagccgggt
     8881 tgcggatccc cggtcgcaca gccgagcagg tcgaattcgc gaccgagcgc gttcgatgcg
     8941 gtaagcccgg cggccggaat cgcgttggtg atcccggcgt tcagcatcgc ctcgcgcatc
     9001 tcggcgaagt ccgggatgta gccgacctgg tttgcgatcg cgagcgttgc gccgaactgg
     9061 ggcgtgatca acggctcgac gtactggacg ttctgggaag ccgcccgctg ggtcacctgg
     9121 gccaggccgt caccctgccg gccgctgaag gcggaaccga agaggtcgaa ggtcgcgaag
     9181 aagtggttgt gaccggaaaa ggcgtcgggc cggaactccc gcatcgacca ggcggcgagg
     9241 atctggtccc agagctgggt gttggtcagg gccgcggtca gcggcacctg actgccggtg
     9301 cagggcggga aggtcgagac gaaggtgacc gggtcgacgc acttgccgtc ctcggcgccc
     9361 cagcggatca tcgcctccgc gtaaaccgcg ccggagaggt gggtgtggag gtctgtgccc
     9421 ttcggcatcc cgagcaggaa ctgccggagg cgctgcggct ggtcacgcag gttgtccaac
     9481 cgcttcgccg ccccggccac cgaggctccg gcactggccg gaaccagggc gagggccaac
     9541 aggccaagga tcagcagccg gaagaaggga cgggacttca cagcaggatc gtattcgctg
     9601 ccgggatcag atccccgggg tggcgtcgat ccttccggcc tcgatctcgg cggtgaacgc
     9661 cttgaagtct tcctggttct gcttcgcgta gctgaggctg aaatcggcga tcgcctggtc
     9721 gaagctgtcg ttcttcccga ggtagtcggc gatcccgacc cggtcaccgg tccgggcatg
     9781 ggccagggcc agggtgccgc cgcaggcccg gacgtattcc gtcagaccca gctcatcgag
     9841 gctgccgatg tcaatcgaga gctttccgtc ccagagctgg cgaatgtaga agtcccgttc
     9901 gtttccatcc ggtcccttcg cgcggatcca gccgaggaag aggtcgctgg ccgcctgggt
     9961 cagccgctgg ccctcgacta cccggcggcc ctgcaggcgg tacttggaag cgccggcgta
    10021 aggggcgagc acggacgcct gggcttcctt gacctgaagc accagcgggt ccttgcgccg
    10081 gcgggaaatg aagagcagca cccaggcccg ggtgccgacc gagccgaccc cgaccacctt
    10141 gcgggccatg tcgacgaatt cgtagtgctc gaagagatat ttgcggtcgg cggcgagggt
    10201 gtcggcgtac tggcccatca gctgctcgag atatccccag atcatgtcga tgtcgtttac
    10261 gcccagtgac gcggcgagct ccgatgcggg gacgagcagg ggcggccggc tggcgaaccg
    10321 cggctcgccc tccgccgagc cttcggtcag cttgcgctgg gcccggctgc tgtccttccg
    10381 gctggccttc cgggtgtccc ggcggaggct gactccccag tcggttccgt tctcctcttc
    10441 gaggaactcg atcatccgct cgacgtcaag ccgctcgtac cagacatcga gatagctctt
    10501 ttcggcaagt tcacgcatca cccgccggta attggccatc ccggccagga cgatcctgcg
    10561 ggcgaccttc ttgctggcgc cgttctcccg ggcggcaatc gcggcgctgg cggccatccg
    10621 cttgacgtcc cattcccagg gggcggggag ggtctcgtcg aagtcgttca ggtcgaagac
    10681 gagcttgcgg tccggcgcgg cgtagccccc gaagttcgag atgtgggcgt caccgcaggc
    10741 ctggacccgg accccggtgg ccggggtgtg ggccaggtcg gcggccatga tcgcggcgcc
    10801 gccccggtaa aaggcgaagg gggaggccga catgcggccg tacctgatcg gcagaaggtc
    10861 ctggacccgg ctctcgccct gggccagcag gatcttcagg gggtcacggc ccggcgccgg
    10921 ctcccagtcc gcgtggcttt ccctcgaagc cttcctgcgc agttccttcc cggtgaatcg
    10981 aggggagtcc tgggtgaggg tggaggtgcc tgatgcgctc gtcatggcgt tgaggatatt
    11041 ggcgccggtc gtctgtttct cattccaacc ggatgaatcg ccacccggcc cgaatgaacc
    11101 acttttcccg cggggaggta tctggccgat ggcgtgtaaa ggcggtttca cgggtcttcc
    11161 cgaactgaaa tcccaaggag gagcaacaaa tgagcggtcc aagcggtacc ggcagtcacc
    11221 tcgacattgg cggggtgttc gaaaagaccg gagacatcta ctcgaaggcc
//
LOCUS       DAOQKN010000075        11215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_2719_length_11215_cov_5.440771, whole genome shotgun sequence.
ACCESSION   DAOQKN010000075 DAOQKN010000000
VERSION     DAOQKN010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 11215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11215
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2719_length_11215_cov_5.440771"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1399)
                     /locus_tag="PLJ64_06590"
     CDS             complement(<1..1399)
                     /locus_tag="PLJ64_06590"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNC93182.1"
                     /translation="MPSGSEESRLVRAAASGDGSAFASLYETYERPIFNYVLRLLGDR
                     HEAEDATQDAFIRVMAKLPELDLDQFEFAPYLYTAARNAGYDVIAKRKKVDLSGSAPD
                     EAAAPFRGELSDLEMDPERSALASSQEAAVKAANDRLPERQREVLALRELDGLSYDEI
                     ASVMEMKSNAVAQLISRARLGLRKEMRVGAAASVAVASPECERAHGEMACRMDGQVKG
                     FDDEWLELHLAGCSNCRVASEEMAEAGVSYRAWAPVVPAAWLFREVMAKASGINGHDW
                     SGIERPEPADGAGSSDADGEGPETGGASGAVEAGRSSRARRALIAGAGSVLVAAVLAT
                     VLLAEANAPEKVAVVRDSLVRSKFKPEKASAKETKKGKAHARKANRNGQGPGGEQTGE
                     QTGPIDPASTNPSGDGGSGGGGNGPRSNGNTTGGGQNPSGSSGSDPVPTPPEPADPVV
                     PSNPTTPAPTDPTPDP"
     gene            complement(1472..3205)
                     /locus_tag="PLJ64_06595"
     CDS             complement(1472..3205)
                     /locus_tag="PLJ64_06595"
                     /inference="COORDINATES: protein motif:HMM:TIGR04534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93183.1"
                     /translation="MYPEIHRLRFVLAAASAITLLVISGMLMLGVTNADSAMVSHRFT
                     DINPGASSANPYSFTRVGGDLYFTASDGQHGSEIWRLSDGRTEMVADVNPGKHGSEPG
                     YLTAFQGQLYFRAYSEATGVEMWRTDGETTELLYDIAPGDESSEPYSPTVLGDRLIFT
                     ADNGTSGSEPWMFDGNTLALVQDLRAGAVGSYPAGYTALGDYLYFRADNGVLGTELYR
                     TDGNSATLVANISPGASSSGPEDFTRFGDYLYFSATNGVGDEMWRTNGSSTAIVKDLY
                     PGANGSSPTHFTVMGDHLYFRATDGVTGLELWRTDGAVPAANVEPVADILPGAGSSYP
                     GQFAVMGGHLYFQAENGPVGEELWRTAGTGAEMVGDIAPGPEDSNPAGLITFGNSVYF
                     IANDGAHGSEVWSTNGGQPRLVRDILEGSDGSSPNQFTVYGNSIYFSADDGVSGTEIW
                     RVTGDSTVKFKLPSAAVRVDRAGRATLKVRCAASEISGPCRGTVVVKTRSKVKTGGKR
                     RQVVIGKALFTAEPGKAGRVSLKLSPKVRRLVRKSGQARRVSVRVKARDGVGNTSTLT
                     RDARLTGPGAR"
     gene            complement(3359..4711)
                     /locus_tag="PLJ64_06600"
     CDS             complement(3359..4711)
                     /locus_tag="PLJ64_06600"
                     /inference="COORDINATES: protein motif:HMM:TIGR04534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93184.1"
                     /translation="MRPLARIAAVAVALISAGLIAGTAQAAVRAELAADVDPGPSSSR
                     PSKLTAVGDSVYFYADGGPDRQGLWRTNGRLTQLVRPVGDPDLRIYWTEKVAFQGRLY
                     LMGSETGNNYRLWRTVPGGGRIEPVPGWPKELKAGSKPTVANGLLFFRGGDGLHKNEL
                     WKYDGERASLVKDINPGPAGSFPGYITGLGNQVIFNATDGVHGGELWRSDGTASGTRM
                     IGEINPGAKGTNMAWLTRVGDSVYLTADTPGHWLEPWRTDGEQLGLVAAEHEGPEWDN
                     ITSFAGLGGKVYFTANNELWESDDAWPEAEVHPIADDPDFPTTAKNAPVAAGNRIFFA
                     SFAGDWTGTMQSWPSFYDGEKIVTLTDPEEEFDYPWGYPQFFKEFGGRVFFVGGDTLH
                     GTELRVVSGRHYRLFADILEGDSSSRPRGFARSKHFMYFVADDGIHGREIWRLRRTAG
                     "
     gene            complement(4708..6363)
                     /locus_tag="PLJ64_06605"
     CDS             complement(4708..6363)
                     /locus_tag="PLJ64_06605"
                     /inference="COORDINATES: protein motif:HMM:TIGR04534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93185.1"
                     /translation="MRAKMESRFRWKAVLGLAFFACLIMVPPASAGIAARLALDVKPG
                     ASGITSTGFSSYDQLTPIGDQLYFTPYFARYGRELWRTDGRTLEMVRDIDPGPGYGYR
                     SEQKPVEFQGRYYFQANSPGTGDELWRLSPDGSDAELVADIYPGPESGYPRALTVFKG
                     QLYFAAGTPASGQELFRYDGTTVEQFTDIKPGAGDAWAGFGKDTILDGYAYFLADDGV
                     HGMELWRTDGENTELWLDATPGAEGSYFFSLKAAGGRLYFATRTDYGLNTRAWRSDGV
                     TNDVLGNFEIWQTTQVGQRVYLLSGHSLMVVTGPDRQPEYKSLPFSAEVDNLTAIGNS
                     LYFTATDRVHGSEPWRIKDGKLELLGSEAYDDQGSIGWYPGQTGPFVEFDGEAFITMS
                     DSRYGSELWKVQDGKAVLFKDILPGRDSGLPGLYTHFGDEVYFIADDGVHGHELWHLF
                     EDDEVGIVVPAQSPKIRNQATSISLGCPASEQAGPCRGSLKISRKGRTLGRGSFRIGA
                     GHTGKPVVKLGPRVAAGPVSLEVTVRDDAGNSKTVSRKLRLSR"
     gene            complement(6459..7412)
                     /locus_tag="PLJ64_06610"
     CDS             complement(6459..7412)
                     /locus_tag="PLJ64_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNC93186.1"
                     /translation="MVGPTEINPEGAERDDSPLATIKRSGTEFIEDGMTDWAAALTYY
                     GLLSLFPMLIALVSIIGLFADPATTTEKLTEIVSNLGPDTAADTFKGPVEGLTSNRGA
                     SGVTFVIGLVLAIWAASGYIGAFSRASNVIFETREGRPFWKLKPLQLGVTLAMVLILA
                     LLAIGLVMTGPVVRAVAEPLGIGSTAVDVWNYAKWPVMIVLVLTLFAVLFHFSPNVRT
                     GRFRFFTPGAGVALLVWILASAGFAFYVGNFGSYNETYGTLGGFVVLLIWFWISNLAV
                     LFGLELNSEFERSRQISAGVGGAERQLQLDPRAEPKPKKTA"
     gene            complement(7440..8183)
                     /locus_tag="PLJ64_06615"
     CDS             complement(7440..8183)
                     /locus_tag="PLJ64_06615"
                     /inference="COORDINATES: protein motif:HMM:NF016240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HNC93187.1"
                     /translation="MIGPTDVEIRVLGCLIEKQRTTPDQYPLTLNSLRLACNQSTNRE
                     PVVDYDEAEIRKALGELGRRRWIRGASGHTSRSPKYRHLLDQELSLSEDQISVLAVLM
                     LRGEQTPGQLKQRTERMFGFHDLDALQTTLDALSSRELIERLPRRPGQKEDRYRQLLG
                     GSHLEDEAAEQAGHPPFEAETSGGDEPPNREANAPGTPVPAAEPLPVPVDTPETERIE
                     TLERQIAGLNGELAELRGLVDELRRELGV"
     gene            complement(8180..9181)
                     /gene="meaB"
                     /locus_tag="PLJ64_06620"
     CDS             complement(8180..9181)
                     /gene="meaB"
                     /locus_tag="PLJ64_06620"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HNC93188.1"
                     /translation="MGRTSPSVEELKEGIARGDRGALSRAITLAESRRPDDQEHAQEL
                     IADLLPETGEAIRVGITGVPGAGKSTAIEALGLELIRHGHKVAVLTIDPTSARTGGSI
                     LGDKTRMHKLSVEEDVFIRPSPSGGVLGGVARKTREAMLLCEAAGFDVIIVESVGVGQ
                     SEAELAEMVDCLMLLLVPGAGDELQGIKRGIMELADLIVVNKADGDRVALAKRARGDY
                     RHALRMLPPSTPGWETPVQIASAAEGTGLKEIWESVEAHRALLEESGLLHERRRQQTE
                     RWLDSMIEEAVLAAVHSRQGVDATIKQARIDVDAEKITVPQATRKVIEAAGLAGEQK"
     gene            complement(9181..>11215)
                     /gene="scpA"
                     /locus_tag="PLJ64_06625"
     CDS             complement(9181..>11215)
                     /gene="scpA"
                     /locus_tag="PLJ64_06625"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HNC93189.1"
                     /translation="EGIDFLDTKPGFAPYLRGPYPSMYVNKPWTIRQYAGYSTAEESN
                     AFYRRNLAAGQKGLSIAFDLATHRGYDSDHPRVAGDVGKAGVAIDCIYDMRTLFEGIP
                     LEEMSVSMTMNGAVLPILSLFIVAGEEQGVPHDQLTGTIQNDILKEFMVRNTYIYPPK
                     PSMRIVSDIIGYTSQEMPRFNSISISGYHMQEAGASADLELGYTLSDGVEYVKAALES
                     GLDVDKFAPRLSFFWAIGMNFFMEVAKMRAGRLLWANLMRQFNPQNPKSLSLRTHCQT
                     SGWSLAAQDAYNNVVRTSVEAMAATQGHTQSLHTNALDEALGLPTDFSARIARQTQLF
                     LQQESGTTRVIDPWAGSYYVEYLTNELARKALAHMDEIDEYGGMTEAIAAGIPKMRIE
                     ESAARTQARIDSGKQTVVGVNSYRPEQDTWVEVLKVDGDEVRRAQIAKLERLRGERNE
                     DDVRQALEHLTKAADSGEGNLLDLGVKAARVYATAGEISEALEKVYGRHSADIKVIGG
                     VYQGEVGVDTESFADTKRLVERFEEVEGRRPRILVAKMGQDGHDRGQKVIATAFADLG
                     FDVDIGPLFQTPEEVARQAIEADVHVVGVSTLAAGHLTLVPALRKALDELDRQDIAIV
                     AGGVIPPADFDELKELGADLIFPPGTVIGEAATELLLLLGERLDLDIQTGSPS"
BASE COUNT         1756 a         3714 c         3757 g         1988 t
ORIGIN      
        1 gcgggtccgg ggtcggatcg gtcggcgccg gggtggtcgg gttggagggc acgaccgggt
       61 cggctggttc gggaggagtt ggaaccgggt cggatcccga cgaaccggag gggttctgcc
      121 cgccgccggt cgtgttgcca ttcgagcgcg ggccgtttcc gccacctccg ctgccgccgt
      181 cacccgacgg gttggttgag gccgggtcga tcggtccggt ctgttcaccg gtctgctccc
      241 cgcccgggcc ctgaccgttc ctgttggcct tcctggcgtg agccttgccc ttcttcgttt
      301 ccttcgcgct ggccttttcc ggtttgaatt tcgacctgac cagcgagtct cgaaccacgg
      361 ccaccttctc cggcgcgttg gcttcggcca gcaggacggt cgcgaggacg gcagcgacca
      421 gaaccgaacc ggcgccggcg atcaacgccc ttcgcgcacg gctgctccgt ccggcctcga
      481 ccgcgccgga cgcgcctccc gtttccggcc cttcgccatc cgcgtcgcta ctgccggcac
      541 cgtcggccgg ctccggccgc tcgattccgg accagtcgtg gccgttgatc ccgctggcct
      601 tggccatgac ttcgcgaaac agccaggcgg ccgggacgac cggtgcccag gcccggtagg
      661 aaaccccggc ttcggccatc tcttcgctcg caacccggca attcgagcag ccggccagat
      721 ggagttcgag ccactcgtcg tcgaagccct tgacctgacc gtccatccgg catgccattt
      781 cgccgtgggc ccgctcgcat tccggggatg cgaccgcgac ggaggcggcc gcaccgaccc
      841 gcatctcctt gcgcaggccg agccgggccc tggagatcag ctgggcgacc gcgttcgact
      901 tcatctccat gaccgaggcg atctcgtcgt agctcagccc gtccagttcc cgcagggcga
      961 ggacctcgcg ctggcgctcg ggcaagcggt cgttggccgc cttgaccgca gcctcctgcg
     1021 aggaggcgag cgcggagcgc tccgggtcca tctcgagatc cgaaagctcc ccgcggaaag
     1081 gcgccgccgc ctcatccgga gccgacccgg aaagatcgac ctttttacgc ttggcgatca
     1141 cgtcgtaacc ggcgtttctg gcggccgtgt agaggtaggg agcgaactcg aactggtcga
     1201 gatcgagctc cggcagcttg gccatgaccc tgatgaaggc gtcctgggtt gcgtcctcgg
     1261 cctcgtgccg gtcgccgagc aaccgcaaca cgtaattgaa gataggccgt tcgtacgtct
     1321 cgtacaaaga cgcaaacgca gagccgtccc cggaagcggc agccctgacg aggcgtgatt
     1381 cctcggaccc ggagggcatg gtgccaatgt aacaccgctc cgggtccgag ggatcactct
     1441 gggcctccct ggctgccaaa cggccgggcg cctaccgcgc gccgggaccg gtcaggcggg
     1501 cgtccctggt cagcgtcgag gtgttgccga ccccgtcccg ggccttgacc cggacgctca
     1561 ccctgcgagc ctggccggac ttccggacca gccggcggac cttcggcgaa agcttcaggc
     1621 tgacccggcc ggccttgccc ggctccgcgg tgaacagggc cttcccgatc acgacctggc
     1681 gacgcttgcc acccgtcttg accttgctcc tggtcttcac caccacggtt ccgcggcagg
     1741 gtcccgagat ctcggaggcc gcgcaacgga ccttcagggt cgcccgaccg gcccggtcca
     1801 cccggacggc cgcggagggc agcttgaact tgaccgtgct gtcaccggtg acccgccaga
     1861 tctcggtgcc ggaaactccg tcgtcggccg agaagtagat cgagttcccg tagacggtga
     1921 actggttggg actgctgccg tcgctgcctt cgaggatgtc ccggaccagg cgtggctggc
     1981 caccgttggt ggaccagacc tcggatccgt gggctccgtc gttggcgatg aaatagacgc
     2041 tgttaccgaa ggtgatgaga ccggccgggt tcgaatcctc ggggcccggg gcgatgtcac
     2101 cgaccatctc ggcgccggtt cccgccgtgc gccagagttc ctctccgacc gggccgttct
     2161 cggcctgaaa gtagaggtgg ccacccatga ccgcgaactg gccggggtag gagctgccgg
     2221 caccgggcag gatgtccgcc acgggctcga cgttcgcggc gggcacggca ccgtcggtgc
     2281 gccacagttc caggccggtg accccgtcgg tcgcccggaa gtacagatgg tcgcccatca
     2341 ccgtgaagtg cgtcggcgag gatccgttgg ctccggggta gaggtccttg acgatcgccg
     2401 tggaggaccc gttggtccgc cacatctcgt cgccgacccc gttggtcgcc gagaagtaga
     2461 ggtagtcgcc gaagcgggtg aagtcctccg gaccggaact ggaagctccc ggcgagatgt
     2521 tcgcgaccag ggttgccgag ttgccgtcgg tccggtagag ctccgtcccg agcacgccgt
     2581 tgtcggcccg gaagtagagg tagtcaccca gcgccgtgta gccggcgggg tacgaaccga
     2641 ccgcacccgc acggaggtcc tggacgaggg ccagggtgtt gccgtcaaac atccagggct
     2701 cgctgccgga agtgccgttg tcggcggtga agatcagccg gtcgccgagc acggtcgggc
     2761 tgtacggctc ggatgactcg tcaccgggag cgatgtcgta gagcagttcg gtcgtctcgc
     2821 cgtcggttcg ccacatctcg accccggtcg cttcggagta ggcccggaag tagagctggc
     2881 cctggaacgc ggtcaggtag cccggttcgg agccgtgctt gccggggttg acatcggcga
     2941 ccatctcggt cctgccatcg ctcagacgcc agatctcgct gccgtgctgg ccgtcgctgg
     3001 ccgtgaagta gaggtcgccg ccgacccggg tgaagctgta cgggttggcg ctgctggccc
     3061 cgggattgat gtcggtgaag cggtgcgaga ccatcgccga gtcggcgttg gtgacaccca
     3121 gcatcagcat gccagagatg accagcaggg tgatcgccga agcggccgcc agtacgaatc
     3181 gaagccggtg aatttctggg tacatcgtga ttcctctctt ggtgattgcg gcttcgaaaa
     3241 gccctttcat ccgtgtaacg aggaaccggc cggtttctta cgcgggcccg gggaaaactt
     3301 ctgcgaagcc cggtttccgg ggcaggcgag cgacccgccg ggccgggcgc cggggacttt
     3361 agcccgcggt ccggcgcagg cgccagatct cgcggccgtg gatgccgtca tcggcaacga
     3421 agtacatgaa gtgcttcgac cgggcgaacc cgcgcggccg gctgctgctg tccccttcga
     3481 ggatgtccgc gaacagccgg tagtgacggc cgctgacgac ccgcagttcg gtgccgtgga
     3541 gggtgtctcc gccaacgaag aagacccttc ccccgaactc cttgaagaac tgcgggtagc
     3601 cccaggggta gtcgaattct tcttcggggt cggtcagggt gacgatcttc tcgccgtcgt
     3661 agaagctggg ccagctctgc atggttccgg tccagtcccc ggcgaaagag gcgaagaaga
     3721 tccggttccc ggccgcgacc ggggcgttct tggccgtggt cgggaagtcc gggtcgtccg
     3781 cgatcgggtg aacctcagcc tccggccagg cgtcgtcgct ttcccagagt tcattgttcg
     3841 ccgtgaagta gaccttgccg cccaggcccg caaacgaggt gatgttgtcc cattccgggc
     3901 cttcgtgttc ggctgcgacg agtccgagct gctctccgtc ggtgcgccag ggctcgagcc
     3961 agtggccggg cgtgtccgcg gtgagataga ccgaatcccc caccctggtc agccaggcca
     4021 tgttggtgcc cttggcgccc ggattgattt ccccgatcat ccgggtccct gaagcggttc
     4081 cgtcggaacg ccagagctcg ccgccgtgga ccccgtcggt ggcgttgaag atcacctggt
     4141 tgccgaggcc tgtgatgtat ccgggaaagg aaccggcggg gcccggattg atgtccttga
     4201 ccaggctggc cctttcgccg tcgtacttcc agagttcgtt cttgtgcagc ccgtcaccgc
     4261 ccctgaagaa gagcaggccg ttggcgacgg tcggcttaga ccctgccttc aactctttcg
     4321 gccagcccgg aaccggttcg atccttcctc ctcccgggac ggtccgccag agccggtagt
     4381 tattcccggt ttccgatccc atcaggtaaa gccggccctg gaaggcgacc ttctcggtcc
     4441 agtagatccg caggtcggga tcgccgaccg gccggaccag ctgggtcagc ctgccattgg
     4501 tgcgccagag gccctgccgg tccgggccgc cgtcggcgta gaaataaacg gaatcaccga
     4561 ctgcggtcag tttgctcggc cgggagctgc tcgggcccgg gtcgacgtcg gctgcgagtt
     4621 cggcccggac cgcggcctgg gccgttcccg cgatcagacc ggccgagatc agggccacgg
     4681 ccacggccgc aatccgtgcc agcggcctca tcgggagagc ctcagcttgc ggctgaccgt
     4741 tttcgaattc ccggcgtcgt cgcgcacggt gacctcaagc gaaaccggac cggccgcgac
     4801 ccggggcccg agtttcacga ccggcttccc ggtgtgaccc gccccgatcc ggaagcttcc
     4861 cctgccgagg gtcctgccct tgcgcgagat cttcaggctg ccccggcagg gcccggcctg
     4921 ctccgaagcc gggcaaccga ggctgatgct cgtggcttgg ttgcggatct tcggcgactg
     4981 cgccgggacc acgatgccga cctcgtcatc ctcgaaaaga tgccagagct catgcccgtg
     5041 tactccgtcg tcggcgatga aatagacctc gtccccgaag tgggtataga ggccgggcaa
     5101 tccggaatcc cggccgggca ggatgtcctt gaacagcacc gccttgccgt cctgcacctt
     5161 ccagagttca ctcccgtacc gggagtcgga catggtgatg aacgcctcgc cgtcgaactc
     5221 gacgaacggc cccgtctgcc ccgggtacca gccgatcgaa ccctggtcgt catacgcctc
     5281 cgatcccaga agttcgagct tgccgtcctt gatccgccag ggctcggacc cgtggacgcg
     5341 gtcggtggcc gtgaagtaga ggctgttgcc gatcgcggtc aggttgtcca cctcggcgct
     5401 gaaaggaagg ctcttgtact cgggctgacg atcgggtccc gtcaccacca tcaggctgtg
     5461 gcccgagagg aggtagaccc tttggccaac ctgggtcgtc tgccagattt cgaaattgcc
     5521 caggacatca ttggtcacgc cgtccgagcg ccacgcgcgg gtattcagtc cgtagtcggt
     5581 ccgggtcgcg aagtagagcc ggcctcccgc cgccttcagg ctgaagaagt acgaaccctc
     5641 ggctcccggc gtcgcgtcca gccagagctc ggtgttctcg ccgtcggtcc gccagagttc
     5701 catgccgtga acgccgtcgt cggccaggaa gtacgcgtaa ccgtcgagga tcgtgtcctt
     5761 gccgaacccg gcccaggcat ctcccgcgcc gggcttgatg tcggtgaact gctcgacggt
     5821 ggtgccgtcg taacggaaaa gctcctgccc ggaagcgggg gtgccggccg cgaaatagag
     5881 ctggcccttg aagaccgtga gtgcgcgtgg atatccgctc tccggaccgg gatagatgtc
     5941 agcgaccagc tcggcatcgg agccatccgg ggagagtcgc cagagctcgt cgccggtgcc
     6001 gggggaattc gcctgaaagt agtagcggcc ctggaattcg accggtttct gttccgatct
     6061 gtagccatat ccgggtccgg gatcaatgtc ccgaaccatc tccaaagtcc gaccatcggt
     6121 ccgccacagc tcgcggccgt accgggcgaa gtagggggtg aagtagagct ggtcgccgat
     6181 cggcgtcaat tggtcatacg aactgaagcc ggtagacgtg attccactgg cgccgggctt
     6241 cacgtcgagc gccagccttg cggcgattcc ggccgaagcc ggggggacca tgatcaggca
     6301 tgcgaagaag gccaggccga ggacggcctt ccagcgaaat cgtgattcca tctttgccct
     6361 cattggcttc tcctgactgg tcggccgggc cagtggcggc gccgcaccgc ggacgatact
     6421 gctgaaaccc ccgtgcaacc gggaagtttc gactcgcttc aggccgtctt cttcggcttt
     6481 ggttcggccc tcggatcaag ctgaagctgc cgttcggccc cgccgacccc cgccgaaatc
     6541 tggcggcttc gttcgaactc ggagttgagc tccaggccga acagcacggc aaggttggag
     6601 atccagaacc agatcagcag gacgacgaaa ccgcccagcg tgccgtaggt ctcgttgtag
     6661 gagccgaagt tgccgacata gaaggcgaag ccggccgaag ccaggatcca gaccagcagc
     6721 gccaccccgg cccccggcgt gaagaaacga aagcggccgg tccggacgtt tggcgagaag
     6781 tggaagagca cggcgaagag agtcaggacg agcacgatca tcaccggcca cttggcgtag
     6841 ttccagacat cgaccgcggt cgacccgatc ccgagtggtt cggcaaccgc ccgaaccacg
     6901 ggaccggtca tgaccagacc gatcgccagc agcgccagga tcagcaccat cgccagggtg
     6961 accccgagct gaagcggctt caacttccag aacggcctgc cttcacgggt ttcgaagatc
     7021 acgttcgagg cccggctgaa ggccccgatg taaccggagg cggcccagat cgccagaacc
     7081 aggccgatca cgaaggtgac cccggacgca ccgcggttgg aggtgaggcc ttcgaccggg
     7141 cccttgaagg tgtcggccgc ggtgtcgggg cccaggttgg agacgatctc ggtcagcttc
     7201 tcggtcgtgg ttgccggatc ggcgaacagg ccgatgatcg agaccagggc gatcagcatc
     7261 ggaaacagcg aaagcagccc gtaataggtg agcgcggccg cccagtcggt catgccgtcc
     7321 tcgatgaact ccgtaccgct gcgcttgatc gtcgcgagcg gcgagtcgtc gcgctcggct
     7381 ccctccggat tgatctcggt cgggccgacc atggaatacc tgtaccccga aagtggaact
     7441 cagaccccga gttcccgccg gagctcgtca accagtcccc tgagctcggc cagctcgccg
     7501 tttagcccgg cgatctggcg ctccagcgtt tcgatccgct cggtttcagg cgtatcgacc
     7561 gggaccggca gcggttctgc cgccgggacc ggcgttccgg gggcgttcgc ctcccgattc
     7621 ggtggttcat cgcccccgga agtttcagcc tcgaaagggg ggtggccggc ctgctccgcg
     7681 gcttcgtctt cgaggtggct tccgccgagc agctggcggt agcggtcttc cttctggcct
     7741 ggccggcggg gcaggcgctc gatcagctca cgcgaggaaa gcgcgtcgag ggtggtctgc
     7801 agcgcgtcaa ggtcgtggaa accgaacatg cgctcggtcc gctgcttgag ctggcccggg
     7861 gtctgctcgc cgcgcagcat cagcaccgca agcaccgaga tctggtcctc ggatagcgaa
     7921 agctcctggt cgagcaggtg ccggtacttg ggcgagcggc tggtgtggcc cgaggcgccc
     7981 cggatccagc gccggcggcc gagctcgccc agcgccttgc ggatctccgc ctcgtcgtaa
     8041 tcgacgaccg gctcccggtt ggtcgactgg ttgcaggcca gccgcagcga gttcagggtc
     8101 agcgggtact ggtccggggt cgtccgctgc ttctcgatca ggcagccgag cacgcggatc
     8161 tcgacatcgg tcggtccgat catttctgct cccctgcgag accggcggct tcgatcacct
     8221 tgcgggtggc ctgggggacg gtgatcttct cggcgtccac gtcaatgcgg gcctgtttga
     8281 tcgtcgcgtc caccccctgc cgggaatgga cggcggcgag gacggcttcc tcgatcatcg
     8341 aatcgagcca gcgctcggtc tgctggcggc ggcgttcgtg gagcaggccg ctttcctcga
     8401 gcagtgcccg gtgggcctcg accgattccc agatctcctt gagtccggtg ccctcggcgg
     8461 ccgaggcgat ctggaccggg gtctcccagc cgggagtcga gggcggcagc atgcggagcg
     8521 cgtggcggta gtcaccccgg gcccgcttgg ccagcgccac ccggtcaccg tcggccttgt
     8581 tgaccacgat caggtcggcc agttccatga tgccccgctt gatcccctga agctcgtcgc
     8641 cggcgccggg aaccagtagc agcatcaggc agtcgaccat ctccgccagc tccgcctcgg
     8701 actggccgac cccgaccgac tcgacgatga tcacgtcgaa accggccgcc tcgcagagca
     8761 gcatcgcctc acgggtcttg cgggccaccc cgccgagcac cccacccgag ggagaaggac
     8821 ggatgaagac atcctcctcg acgctcagct tgtgcatgcg ggtcttgtca ccgaggatcg
     8881 aaccgccggt gcgggccgag gtcggatcga tcgtgagcac cgcgaccttg tgaccgtggc
     8941 gaatcagctc aagaccgagc gcctcgatcg cggtcgactt gccggccccg ggcacgcccg
     9001 tgatcccgac ccggatcgcc tcaccggttt ccggcagcag gtcggcgatc agctcctggg
     9061 cgtgctcctg gtcatcgggc cggcggcttt cagccagggt gatcgccctt gagagcgccc
     9121 cgcggtcacc gcgggcgatg ccctccttca gctcctcgac cgaagggctg gttctgccca
     9181 ttacgacggg gagccggtct ggatgtccag gtcgagcctc tcgccgagca gcagcagcag
     9241 ctcggtcgct gcttcgccga tcacggtgcc gggcgggaag atcaggtcgg cgccgagttc
     9301 cttcagctcg tcgaaatcgg ccggagggat cacgccgccg gcgacgatcg cgatgtcctg
     9361 ccggtccagc tcgtcgagcg ccttgcgcag ggccgggacc agggtgaggt ggccggcggc
     9421 aagggtgctg accccgacca cgtggacgtc cgcctcgatc gcctggcggg caacctcttc
     9481 cggagtctgg aagagcgggc cgatgtcaac gtcgaagccg aggtccgcga aggcggtggc
     9541 gatcaccttc tggccccggt cgtgaccgtc ctgccccatc ttggcgacca ggatgcgcgg
     9601 ccgtcgccct tcgacttcct cgaaacgctc gaccagtcgc ttggtgtccg cgaaggactc
     9661 ggtatccact ccgacttctc cttggtaaac cccgccgatc accttgatgt cggcggaatg
     9721 gcggccatag accttttcca aagcttcgct gatctcaccg gcggtggcgt agacccgggc
     9781 ggccttgacc ccgagatcga gcagattgcc ttcaccgctg tccgcggctt tggtcagatg
     9841 ctcgagcgcc tggcgcacgt cgtcctcgtt gcgttcgccc cgcagccgtt ccagcttcgc
     9901 aatctgggca cgccggactt cgtcgccgtc gaccttcagc acctcgaccc aggtgtcctg
     9961 ctcgggccgg taggagttga ccccgaccac cgtctgcttg ccggagtcga tccgggcctg
    10021 ggtgcgggcg gccgattcct cgatccgcat cttcgggata ccggcggcga tcgcctcggt
    10081 catgccgccg tactcgtcga tctcgtccat gtgggccagt gccttgcggg ccagctcgtt
    10141 ggtcaggtac tcgacgtagt aggagccggc ccaggggtcg atcacccggg tggtgccgga
    10201 ttcctgctgc aggaagagct gggtctggcg ggcgatccgg gccgagaagt cggtcggcag
    10261 gcccagcgcc tcgtcgagcg cattggtgtg gagcgactgg gtgtgaccct gggtcgcggc
    10321 catcgcctcg acgctggtcc ggaccacgtt gttgtacgcg tcctgggcgg cgaggctcca
    10381 gccggaggtc tggcagtggg tcctgagtga aagtgacttg gggttctgcg ggttgaactg
    10441 gcgcatcagg ttggcccaga gcaggcgccc ggcccgcatc tttgccactt ccatgaagaa
    10501 gttcatcccg atcgcccaga agaaggagag ccgcggcgcg aacttgtcca cgtcgagacc
    10561 cgattcgagg gccgccttca cgtactcgac cccgtccgaa agcgtgtagc ccagctcgag
    10621 gtcggcgctg gccccggctt cctgcatgtg gtaaccggag atcgaaatcg agttgaagcg
    10681 gggcatctcc tgcgaggtgt atccgatgat gtccgagacg atccgcatcg agggcttggg
    10741 cgggtagatg taggtgttcc gcaccatgaa ctctttgagg atgtcgttct ggatggtgcc
    10801 ggtcagctga tcgtggggca cgccctgctc ctcaccggcg acgatgaata gcgagaggat
    10861 cggcagcacc gccccgttca tggtcatcga gaccgacatc tcttcgagcg ggatcccttc
    10921 gaacagggtc cgcatgtcat agatgcagtc aattgccaca ccggccttgc cgacatcgcc
    10981 ggcaacgcgg gggtggtcgg agtcgtagcc gcggtgagtg gccaggtcga aggcgatcga
    11041 aaggcccttc tggccggccg caaggtttcg acggtagaag gcgttcgatt cttcggccgt
    11101 ggagtagccg gcgtactggc ggatcgtcca gggcttgttc acgtacatcg acggatacgg
    11161 gccccggagg tagggcgcaa agcccggctt ggtgtcgagg aaatcgatgc cctcg
//
LOCUS       DAOQKN010000076        11078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_2770_length_11078_cov_5.667241, whole genome shotgun sequence.
ACCESSION   DAOQKN010000076 DAOQKN010000000
VERSION     DAOQKN010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 11078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11078
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2770_length_11078_cov_5.667241"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="PLJ64_06630"
     CDS             <1..640
                     /locus_tag="PLJ64_06630"
                     /inference="COORDINATES: protein motif:HMM:NF018496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HNC93190.1"
                     /translation="VLLIPPAILQWLIGDSALGGLVSAIVNIFATAWLAGGVVRIVQD
                     VEADGQVDYSVGDLLGSVWPRLLAIVGLQIVVGIIIFFGLIFFIIPGIILALVLVVSL
                     PALVVEDGGIFDSMGRSAALTKDNRMRILAVGILMILILVGIAIVAGLLSAITPIIGA
                     LAMLVLGVLLYPYIYMLTTVLYFRLVELKEGGVVAVEETVIVEEDAGPPPAV"
     gene            complement(772..1818)
                     /locus_tag="PLJ64_06635"
     CDS             complement(772..1818)
                     /locus_tag="PLJ64_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93191.1"
                     /translation="MPAAILAFVAFLLLLSAVETPAEAALPEVLSGRTIVQPSPLEKG
                     PGSLDYSHSGWRVSEGGKGYDAWIAFEPTGPRPARAPLAIVTHGYGEFSGYDRLYELI
                     RHTVLKGNVVIFPRWQISDFDPCPGPFNIKACMDSAVTGIHAALEYLRESPNRVQPDT
                     GRTSYYGYSFGGIITANIANQYRFYGLPRPRAIFLDDPHDGEVAGLGEPALDDSLAGI
                     PSDVKFECHSGADGVLGVPRLIEGSCNAVFPKLTHIPARNKALVMTRTDSWGTPVLSS
                     AHGVCASNQGTADAFDWRFCWRDWDALRSCAFQGKDCHLALGTGPRHSWLGRWSDGRP
                     VTRLTIHKATPLAP"
     gene            complement(1903..2580)
                     /locus_tag="PLJ64_06640"
     CDS             complement(1903..2580)
                     /locus_tag="PLJ64_06640"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC93192.1"
                     /translation="MAVQSPHDLIPRVREGVFFEPLQALPRGRHSLSGEEVRAIQQER
                     ILVSMTELMAAHGAPGIGSNEIARHAGVSLAAFYECFDGKEACALAGYDRFIEVLFHA
                     MNAVDVGSRTRPELVCGLIRAYLETLQSDLVVARAYQVEIDSLGASVRKRRRDSLLLF
                     AHHLWGVSSDDSPTGRGPEQLPWSAYIGIIYTTRQLASDALDTEEKPDLIALSDELAI
                     WVTDLFR"
     gene            2733..4721
                     /locus_tag="PLJ64_06645"
     CDS             2733..4721
                     /locus_tag="PLJ64_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93193.1"
                     /translation="MSKQGEGKGEEDPGVREVTPAEAGGITRRGFLARSAATAATAAT
                     VGSLTGAGGASAAGSESRKVPGSFGEVALPDARQVKADYQRMVDFGPRLPGYEEHLEF
                     CNWIEDEFVKAGLELLPTDQIPYRRWRPGKMSLRVGTTDFDPATTYVRVPGTPAEGIT
                     APMMLAGDGPVDGKIVLVEAAVPTPRTAGLFIAMADYWQWRGHTAFEAAAEEFDRFAF
                     SDWPTIKPYADQGAKAVIFISRASRAEVEGNWSPHQAEVQPIPGFVVDRDMGAELKAL
                     AAGQPEATLKLEADWKDTYVPQITGILPGESDEVLIVNSHTDGQNAFEENGAVALVHL
                     ARHFASLRGSRKLKRTLVFIAWPGHMANVSNRPDDRTTLPEQFGWISSHRDLFDRTVA
                     AVTIEHLGATRWSDDPVEGYHSTGMSDFYAIWTTLGAMQDLCKVALKESDLQRHALLR
                     PPIQVTPGVAWHHRGIPHVAGISAPKYLVRVSENGDIDKLDASLASRQTGFYADVVKS
                     LDSADPAALKAGGEGDSSFNSVSDREYPMTRARSGPAREVVLDAGDGGRLAIYIGGRA
                     AGGRDIGVQMAALDHSLERVTVAVYRDDTLLGKSRPTRVDRKVKRVAIHRSGHRADHR
                     NLHRNRSAARFRDGTYTFVVRQDGKLLGQRNVTYQRRR"
     gene            4788..5885
                     /locus_tag="PLJ64_06650"
     CDS             4788..5885
                     /locus_tag="PLJ64_06650"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HNC93194.1"
                     /translation="MKETTIQDLGGTGRARVFLLAAFVALAGMLGMAGSSKAETHTIT
                     VDNGRLTMGFVFLNNKILPADVPGPLDPNISNTSGSGTIAVETSGSTATVAEADFNMP
                     IVWIPDPTAGGAPIPMKFGPKGDLTGTWDAGTGQLSLTGTLQVDVIWGFDEGGAKICR
                     FTAPNTTWTTAPNAISPGVAFNGVGGLSGNGAISAFWDELPAGESINGGDCGKPNAVV
                     RDPGAVWLSSGIAEVPAPPTCQELGKDGLWPDCVDIDWPDPDPVVEITKVTVTKGTVT
                     AGQKAKIKVNVTNSGDLAATPITIKLASSSKQVKVPGQVKLNVPAGKTATASITVTAT
                     KNARGKATITASTSGKSGKAVVTVKKPRKRK"
     gene            complement(5984..6445)
                     /locus_tag="PLJ64_06655"
     CDS             complement(5984..6445)
                     /locus_tag="PLJ64_06655"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC93195.1"
                     /translation="MIEIRPVAPGEFPLFEPLFVLYQEFYEVKDIDRGRNREFFSRFL
                     VGEDGVGSADGWLLGAFDGEEAVGFGCFYRHKSSLSATDVVLMNDLFVTEAARGGGTG
                     RALIEAGVELARESGASHLTWATAPDNHTAQRLYDSTGAHKSTWLEYEIEA"
     gene            6649..7947
                     /gene="clpX"
                     /locus_tag="PLJ64_06660"
     CDS             6649..7947
                     /gene="clpX"
                     /locus_tag="PLJ64_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNC93196.1"
                     /translation="MAGPTDTNEQLLCSFCGKSQRQVKKLIAGPGVYICDECIDLCNE
                     IIDEESVSAAAPAGLDLDRLPRPQEINSVLDEYVVGQETAKRALSVAVYNHYKRVRMA
                     EEGDGDVELQKSNIMLLGPTGCGKTLLAQTLARILNVPFAIADATALTEAGYVGEDVE
                     NILLKLIQAADYDVAKAETGIIYIDEIDKITRKADNPSLTRDVSGEGVQQALLKILEG
                     TTASVPPQGGRKHPHQEFLTIDTTNILFICGGSFSELEKVIERRVDHKGIGFGAAITE
                     QAHDDPGELFEQVLPEDLVEYGLIPEFIGRLPVVAALHQLDQTDLVRILTEPKHALVR
                     QFQRFFEFDDIELVFADDSLEAIADKALARETGARGLRSIIEDALLDVQFELPSREDV
                     SKCVVTRESIEKGQSPMLVTEPSPVADPHDEDLFGEEQTA"
     gene            7973..8767
                     /locus_tag="PLJ64_06665"
     CDS             7973..8767
                     /locus_tag="PLJ64_06665"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNC93197.1"
                     /translation="MGAPPLDRDSSDLQVTWGGHATVLIEIDGVRLVTDPVLRSRVVH
                     LRRQVPDPGTAFIEGIDAVLISHLHFDHLDIPTLKRFDRGVKVIVPGGAGPMIRKHGF
                     REVIELMEGEATEVGPVTVLAVPADHDGRRHPFGMEIDAIGFVVEGSRRILFAGDTDL
                     HPRLGELADSLEVALLPIWGWGHRLGPGHMDPRGAAQAAATLRPKITVPIHWGTYFPI
                     GMKGIRGALLEKPVKAFEQQMEEIAPGLEIEVLRPGESLSRPAASS"
     gene            complement(8724..10784)
                     /locus_tag="PLJ64_06670"
     CDS             complement(8724..10784)
                     /locus_tag="PLJ64_06670"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNC93198.1"
                     /translation="MEGLNEPETRRHPGKLFARILVVVLIDAAALLLIEAVLPGFDMH
                     GRFAALPTALGVGLVNALIWPVLSRFTLKLSVLTLGLWGLFLNAVLIGFALMAMPWVR
                     IEGPFEAIVISFGMAILTSLFSSLFAIDEDSTWYYNVVRTQLRRRGQVIETDEPGIVF
                     LEIDGLAHDILRRAMTNGNAPAMAAWVRDGSHKLEGWETDWSSQTGACQAGILHGNNH
                     DMPAFRWWEKETGRALVTNHPKDAAEMEARQSDGNGLLADDGASRANILSGDAPHSML
                     TMSTVMKRRGKIGQDYAAYFARPYGVIKTFIHTIAEIVRERRDNRRQERTGIEPKIER
                     SRVYAIMRAWATVIQLDLQISGVVGDILAGRPSIYTTFLAYDEVAHHSGIERPESLST
                     LAKVDREIDRIRRVVAEAPRPYHLVVLSDHGQSQGATFLQRYGVTLEEVVEKATGKEA
                     IGSGEEGEQNPRAYITASAEEASHEDGAIGSAATALARRDPNRDDLGLEGGPDEVAGG
                     EGKHEIPELSVMASGCLGLVTFPREPGRLTRERIDELYPGLLDTLVSHPGIGFVMVDS
                     EHGGVALGAEGLNYFEINDVEGVDPLAAFGPNTAAHVLRTHRFSNCPDIMINSTWWEE
                     MDEVAAFEELVGSHGGMGGTQSHPFVLHPVDLEWPEEPVIGAEAVHRILKSWLPAGTK
                     TLRA"
     gene            complement(10843..>11078)
                     /locus_tag="PLJ64_06675"
     CDS             complement(10843..>11078)
                     /locus_tag="PLJ64_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93199.1"
                     /translation="QTRAATVKVRFKSNEKGSTFKCRLNKGKWKSCKSPWKTPRLRKG
                     KNTVSIRAIDKAGNRSKVVTRVIRKVSKAKKRR"
BASE COUNT         1939 a         3584 c         3697 g         1858 t
ORIGIN      
        1 tgtcctcctg atcccgccgg caatcctgca gtggctgatc ggtgacagcg ccctcggcgg
       61 cctcgtttcg gcgatcgtca acatcttcgc caccgcctgg ctggccggcg gggtggtgcg
      121 catcgtccag gacgtcgaag cggacggaca ggtcgactac tcggtcggtg atctcctggg
      181 ttcggtctgg ccgcgcctgc tggcgatcgt cggccttcag atcgtggtcg ggatcatcat
      241 cttcttcggg ctgatcttct tcatcatccc gggcatcatt ctcgccctgg tgctggtcgt
      301 ctcgctgccg gcccttgtgg tcgaagacgg cggaatcttc gattcgatgg gccgcagcgc
      361 cgcactgacc aaggacaacc gcatgcggat cctcgcggtc ggcatcctga tgatcctgat
      421 cctggtcggc atcgcgatcg tggccggcct cctttcggcg atcacgccga tcatcggggc
      481 gctcgcaatg ctcgtgctcg gggtgctgct ctacccgtac atctacatgc tgacgacggt
      541 tctttacttc aggctggttg agctgaagga gggcggcgtg gtcgcggtcg aagagaccgt
      601 gatcgtcgag gaggacgccg gaccgccgcc cgccgtgtag gcggcggcaa acttgcgccg
      661 gtccgggtgg gagcccccgg atcagcgcag tcaacaaaag ggccgcggcc ggtgcgcaga
      721 gcaccagccg cggcccttgt tcttttgaga gagagccccg gaggcccggg ttcagggagc
      781 gagcggcgtc gctttgtgga tggtgagccg ggtgaccggc ctgccatcgc tccatcttcc
      841 gagccagctg tgtcgcggcc cggtgcccag cgcgaggtgg cagtccttgc cctggaaggc
      901 acaggaacgc agggcatccc agtcacgcca gcaaaagcgc cagtcgaagg catcggcggt
      961 tccctgattg gaagcgcaga ccccgtgggc cgaactcaga accggagttc cccatgagtc
     1021 ggttctggtc atgaccaggg ccttgttgcg ggccgggatg tgggtgagct tcgggaagac
     1081 cgcgttgcaa ctgccctcaa tcaggcgagg gacgccgagc acgccgtcgg cgcccgagtg
     1141 gcactcgaac ttgacgtcgg aggggatccc ggcgagggag tcgtcgaggg ccggttcgcc
     1201 cagcccggca acctctccgt cgtgaggatc gtcgaggaat atcgcccggg gccggggcag
     1261 cccgtagaac ctgtactggt tggcgatgtt cgcggtgatt atcccgccga acgagtagcc
     1321 gtagtagctg gtccttccgg tgtccggctg gacccggttc gggctttcac gcaggtactc
     1381 cagggcagcg tgaatcccgg tgactgccga gtccatgcag gccttgatgt tgaaggggcc
     1441 ggggcagggg tcgaagtcac tgatctgcca gcgggggaag atcacgacgt tgcccttgag
     1501 gaccgtgtgc cggatcagtt catagagccg gtcgtagccg ctgaactccc cgtagccgtg
     1561 ggtgacgatc gccagcggcg cccgggcggg cctcggccct gtgggttcaa aggcgatcca
     1621 ggcgtcgtag cctttaccgc cttcactgac ccgccagccg ctatggctgt agtcgagact
     1681 tccgggaccc ttttcgagcg gggagggctg gacgatcgtg cggccggaaa ggacctcggg
     1741 gagagcggct tccgcagggg tttcaaccgc tgacaacagc agcagaaagg cgacaaatgc
     1801 aagtattgcg gcgggcatct ctcctcctcg gtcccttcgg cgtaaagtac catacgccga
     1861 tctcgcgaag gcgggattcc gcttccggcc tgaagccggg gatcaacgga agaggtcggt
     1921 gacccagatc gccagttcgt cgctcagggc gatcagatcc ggcttctctt cggtgtcgag
     1981 ggcatcggag gccagctgcc tcgtggtgta gatgatgccg atgtaggccg accagggcag
     2041 ctgctccggg ccgcgcccgg tcggagaatc gtcgctggag acaccccaga ggtgatgggc
     2101 gaagagcagg agggaatccc ttcggcgctt gcgaacggaa gcgccgagtg aatcgatctc
     2161 gacctggtag gcgcgcgcca ccacgaggtc gctctggaga gtctccaggt acgcccggat
     2221 cagcccgcag accagttcgg gccgggttct gctgccgacg tcaaccgcgt tcatggcgtg
     2281 gaagagcacc tcgatgaagc ggtcgtagcc ggccagggca cacgcttcct tgccgtcgaa
     2341 gcattcgtag aaggcggcca gcgaaacacc ggcgtggcgc gcgatctcgt ttgatccgat
     2401 gcccggtgcg ccatgggcgg ccatcagttc ggtcatcgag acgagaatgc gttcctgctg
     2461 gatcgcgcgg acctcctcgc cgctcaggga atgcctgccg cggggcaggg cctgaagcgg
     2521 ttcgaagaag acaccttcgc gaacgcgggg gatcaaatca tgcggggact ggaccgccat
     2581 tggacggaat atacccatgg ccgggggcgg cgcatccggt ttcggccggc gggaatcctg
     2641 cggcccccga ctccgggacc cggcgttccc ttggtcgttg tcacgtctgc tacgatcggc
     2701 gtagaggatt gttcttcaag gagaggatgg agatgtccaa gcagggtgag gggaagggtg
     2761 aggaggatcc gggagtccgt gaggtgaccc cggccgaggc cggcggcatc acccggcggg
     2821 gcttcctggc ccgttccgca gcgaccgctg cgaccgccgc gacggtggga agcctgaccg
     2881 gcgccggcgg ggcgtccgct gccgggagcg agtcccgcaa ggtacccggg agctttggcg
     2941 aggtagccct gcccgatgca aggcaggtca aggccgacta tcagcggatg gtcgatttcg
     3001 gcccccggct ccccggctac gaagaacatc tcgaattctg caactggatc gaggacgagt
     3061 tcgtaaaggc agggctcgag ctgctgccca ccgaccagat cccctaccgc cgctggcggc
     3121 cgggcaagat gtcgctgcgg gtcggcacca ccgactttga tcccgcgacc acctacgtgc
     3181 gggttcccgg cactccggcg gaggggatca cggcccccat gatgctggca ggcgacggcc
     3241 cggtcgacgg gaagatcgtc cttgtcgagg ccgcggtgcc gaccccgcgc accgccggcc
     3301 tcttcatcgc gatggccgac tactggcagt ggcgtggaca cacggccttc gaagcggcgg
     3361 ccgaggaatt cgaccgtttc gccttctcgg actggcccac gatcaagccc tacgccgacc
     3421 agggcgccaa ggcggtgatc ttcataagcc gggcttcgcg ggccgaagtc gaaggcaact
     3481 ggtcacctca ccaggcggaa gtccagccga ttcccggctt cgtggttgac cgcgacatgg
     3541 gggccgaact gaaggcgctc gccgccggcc agcccgaggc gaccctgaag ctggaagcgg
     3601 actggaagga cacctacgtt ccgcagatca cgggcatcct gccgggcgag agcgacgagg
     3661 tgctgatcgt caactcgcat accgacggcc agaacgcctt cgaggaaaac ggggctgtgg
     3721 ccctcgtcca tctggccagg catttcgctt cgcttcgcgg cagccggaaa ctgaagcgca
     3781 cgctcgtctt catcgcctgg cccggccaca tggccaatgt cagcaatcga ccggatgacc
     3841 ggacaacgct gccggagcag ttcggctgga tcagctcgca ccgggatctc tttgaccgca
     3901 ccgtcgccgc ggtcacgatc gaacacctcg gggccacacg ctggtccgac gacccggtcg
     3961 agggctacca ctcgaccgga atgtccgatt tctacgcgat ctggaccacc ctgggagcca
     4021 tgcaggacct gtgcaaggtc gccctgaagg agtcggatct gcagcgccac gcgttgctca
     4081 ggccaccgat ccaggttacg cccggcgtcg cctggcacca ccgggggatc ccgcacgtgg
     4141 ccgggatctc ggcgcccaag tacctggtca gggtttccga gaacggcgac atcgacaagc
     4201 ttgacgcctc cctggcctcc cggcagaccg gcttctacgc cgacgtggtc aagtcgctgg
     4261 actcggccga ccctgcggcc ctcaaggcgg gtggtgaagg cgactcaagc ttcaacagcg
     4321 tgtccgatcg cgagtatccg atgaccaggg cccgctcggg gccggcacgg gaagtggtgc
     4381 tggacgcggg cgacggcggt cgcctcgcga tctacatcgg aggccgcgct gccggcggcc
     4441 gggacatcgg cgtccagatg gcagcactcg accactcgct cgagcgggtc acggtggcgg
     4501 tctaccgcga cgacaccctc ctcggcaaat ccagaccgac gcgcgtcgac cgcaaagtca
     4561 agcgggtcgc gatccaccgg agcggacacc gggccgacca ccgcaatctt cacaggaacc
     4621 ggagcgcggc cagattccgc gacggcacct acacattcgt cgtccgtcag gacggcaagc
     4681 ttctgggtca gcggaacgtc acctaccagc ggcgccgcta ggtacccgga cgagggaaac
     4741 aggactgcga tgactgcgca gcacatgaac agcaacaagg agaggcaatg aaggagacaa
     4801 ctattcagga cctgggaggg acaggaaggg cgagggtgtt tctgcttgcc gcctttgtcg
     4861 cgctggccgg catgcttggc atggccggtt catcaaaggc cgagacccac acgatcacgg
     4921 ttgacaacgg acgcttgacg atgggtttcg tcttcctcaa caacaagatc ctcccggccg
     4981 acgtgcccgg gccgctggat cccaacatca gcaacacctc gggcagcggc acgatcgccg
     5041 tcgaaaccag tggctcgacc gccacggtcg ccgaggcgga tttcaacatg ccgatcgtct
     5101 ggatcccgga cccgaccgcg ggcggcgctc cgatcccgat gaagttcggt cccaaagggg
     5161 acctgaccgg cacctgggac gccgggacgg gtcagctctc ccttacgggc acactccagg
     5221 ttgacgtgat ctggggattc gacgagggcg gcgcgaagat ctgccggttc accgcgccga
     5281 acacgacctg gaccaccgcc ccgaacgcga tcagcccggg agtcgccttc aacggggtcg
     5341 gcggactttc cggcaacggt gcgatcagcg ccttctggga cgaattgccg gccggcgagt
     5401 cgatcaacgg tggcgactgc ggcaagccca acgccgtcgt gcgtgacccg ggtgcggtct
     5461 ggctttcgag cggcatcgcc gaagtcccgg ctcccccgac ctgccaggaa ctgggcaagg
     5521 acgggctctg gcctgactgc gtcgacatcg attggcccga tcccgatccg gtggtcgaga
     5581 tcaccaaggt gaccgtgacc aagggaactg tcacagccgg ccagaaggca aagatcaagg
     5641 tcaatgtcac caactccggg gatctcgccg cgaccccgat caccatcaag ctcgcttcga
     5701 gctcgaagca ggtcaaggtg cccggtcagg tcaagctgaa cgtcccggcc ggcaagaccg
     5761 cgaccgcgtc gatcaccgtg accgcgacca aaaatgcccg gggcaaggcg acgatcaccg
     5821 ccagtacctc agggaagtcc ggcaaggccg tggtgaccgt aaagaagccg aggaagcgca
     5881 agtaacgcga gcgcttcacc cgacgaaagc gcggagtgag ttgcctttct tcacgagcta
     5941 cggagaaggc gactcgctcc gcgtcttttg cgttcggccg ggctcaggct tcgatctcgt
     6001 attccagcca ggtggacttg tgggctccgg tgctgtcgta gagacgctgg gcggtgtggt
     6061 tgtccggggc agtggcccag gtcaggtgtg aggcgccgga ctcgcgggcg agctcgaccc
     6121 cggcctcgat cagggcccgg ccggtccccc cgccgcgcgc ggcctcggtc acgaaaaggt
     6181 cgttcatcag cacgacgtcg gtagccgaaa gcgagctctt gtgacggtaa aagcagccga
     6241 agccgaccgc ttcctcgccg tcgaaggcac cgagcagcca gccatcggcg gaaccgacgc
     6301 cgtcttcgcc cacgaggaag cgggagaaga actcccggtt cctgccccgg tcgatgtcct
     6361 tgacctcgta gaactcctga tagagcacga agagcggttc gaagagcgga aattccccag
     6421 gagcaactgg tcggatctcg atcatgggat cagcgtaccg gcatgatcag agcccttccg
     6481 gcttccggcg ccggccggag aagatgcagg tgttcttgcc ggggaaggga gcagggtatg
     6541 gtcggagtag aaggtgtcca acgtcgcctg cgaccgcagt tgagaggccg cggcggcgct
     6601 tcttctagag tgggaccaag cgagcccgct cacgaccccg gagggtagat ggcaggtccg
     6661 accgatacca acgagcaact tctctgcagc ttctgcggca aatcgcagcg gcaggtgaag
     6721 aagctgatcg ccggtccggg tgtttacatc tgcgacgagt gcatcgacct ctgcaacgag
     6781 atcatcgacg aggagtcggt ctctgctgct gccccggccg ggctcgacct cgaccgcctg
     6841 ccccgcccgc aggagatcaa ttccgttctc gacgagtacg tggtcggcca ggaaaccgcc
     6901 aagcgggccc tctcggtggc cgtctacaac cactacaaac gggtccgcat ggccgaggag
     6961 ggcgacggcg acgtcgagct gcagaagtca aacatcatgc tgctcggccc gaccggctgc
     7021 ggcaagaccc tgctcgccca gacgctcgcc cggatcctca acgtgccatt cgcgatcgcc
     7081 gatgccaccg ccctgaccga ggccggatac gtcggcgagg acgtcgagaa catcctgcta
     7141 aagctcattc aggcggccga ctacgacgtg gccaaggccg agaccgggat catctacatc
     7201 gacgagatcg acaagatcac ccgcaaggcc gacaacccgt cgctgacccg tgacgtatcc
     7261 ggcgaaggcg tgcagcaggc gctgctgaag atcctcgaag gaaccaccgc ctcggtaccg
     7321 ccgcagggtg gccgcaagca tccgcatcag gaattcctga ccatcgacac gaccaacatc
     7381 ctcttcatct gcggcggctc gttctcggaa ctggagaagg tgatcgagcg acgggtcgac
     7441 cacaagggaa tcggcttcgg cgcggcgatc accgagcagg cccacgatga tcccggcgag
     7501 ctcttcgaac aggtgctgcc cgaggacctg gtcgagtacg gcctgatccc cgagttcatc
     7561 ggccggctgc cggtggtggc ggcgcttcat cagctcgacc agaccgacct ggtccggatc
     7621 ctcaccgagc ccaagcatgc tctggtccgc cagttccagc gcttcttcga gttcgatgac
     7681 atcgagctgg tcttcgccga cgattcactc gaagcgatcg ccgacaaggc tctggcccgc
     7741 gagaccggcg cccgggggct gcgctcgatc atcgaggacg ccctgctcga cgtccagttc
     7801 gaactgccct cccgcgagga cgtgagcaag tgcgtggtga cccgcgaatc gatcgagaag
     7861 ggccagtcgc cgatgctggt gaccgaaccg agcccggtcg cggacccgca cgacgaggac
     7921 ctgttcggcg aagagcagac cgcctgactc ttcgccgcgg gatactccct gcgtgggagc
     7981 gccgcccctt gaccgggatt catccgatct ccaggtgacc tggggcggac acgccacggt
     8041 cctgatcgag atcgacgggg tgcgcctcgt caccgacccg gtgctccgca gccgggtggt
     8101 ccacctgcgc cggcaggtgc ccgaccccgg aacggccttc atcgaaggca tcgacgcggt
     8161 cctgatctca catcttcatt tcgaccatct cgacatcccg accctgaagc gcttcgaccg
     8221 cggggtgaag gtaatcgttc cgggcggcgc cgggccgatg atcagaaagc acggcttccg
     8281 cgaagtgatc gagctcatgg aaggggaagc gaccgaggtc ggcccggtta cggtcctcgc
     8341 agtgcccgcc gaccacgacg gtcgccgcca tcccttcggg atggagatcg atgcgatcgg
     8401 cttcgtggtc gaaggttcgc ggcggatcct gttcgccggt gacaccgacc tgcatccgcg
     8461 gctcggtgaa ctggccgaca gcctggaagt ggccctgctg ccgatctggg gctggggtca
     8521 caggttgggt cccggccaca tggacccgcg cggggcagcg caggcggccg cgaccctgag
     8581 gccaaaaatc accgtgccga tccactgggg cacctacttt ccgatcggca tgaaggggat
     8641 ccgcggggct ctgctggaaa agccggtaaa ggccttcgag cagcagatgg aggagattgc
     8701 ccccggcctt gagatcgagg tgctcaggcc cggagagtct ttgtcccggc cggcagccag
     8761 ctcttgagaa tccggtgaac cgcttcggct ccgatcaccg gttcttccgg ccactcgagg
     8821 tcgaccggat ggagcacgaa gggatggctc tgggtgccgc ccatgccgcc gtgtgagccg
     8881 accagctcct cgaaggcggc gacctcgtcc atctcctccc accaggtgct gttgatcatg
     8941 atgtcggggc agttgctgaa ccggtgggtc cgcaggacat gggcagcggt attcggcccg
     9001 aacgcggcca gggggtcgac cccttcgacg tcgttgatct cgaagtagtt caggccctcg
     9061 gcgccgagcg cgacgccgcc gtgttcggaa tcgaccatca cgaagccgat cccgggatgt
     9121 gacaccaggg tgtcgagcag gccggggtag agctcatcga tccgttcgcg ggtgagccgg
     9181 cccggttcgc ggggaaaggt aaccagcccg aggcaaccgg aagccatcac cgacagttcc
     9241 gggatctcgt gctttccttc acctccggcg acttcatccg gtccgccttc gagcccgagg
     9301 tcgtcgcggt tcgggtcccg ccttgccagc gcggtggcgg cgctgccgat cgcaccgtcc
     9361 tcgtggctcg cctcctcggc actggccgtg atgtaggcgc gggggttctg ttcgccttcc
     9421 tcgccggagc cgatcgcctc cttgccggtc gccttctcga ccacttcctc gagcgtcacg
     9481 ccgtaacgct gaaggaaggt agccccctgg gactggccgt ggtccgagag gacgaccagg
     9541 tggtagggcc gcggggcctc ggcgacgacc cggcggatcc ggtcgatctc acggtcgacc
     9601 ttggccaggg tggagaggga ttcgggacgc tcgatcccgg agtggtgggc tacctcgtcg
     9661 taggcgagga aggtcgtgta aatggagggg cgaccggcga ggatgtcccc gaccacgccc
     9721 gagatctgaa ggtcgagctg gatcaccgtg gcccaggccc gcatgatcgc gtagaccctc
     9781 gagcgttcga tcttcggttc gatcccggtt ctttcctggc gccggttgtc ccgccgctcg
     9841 cggacgatct cggcgatcgt gtggatgaag gtcttgatca ccccgtaggg acgggcgaaa
     9901 taggcggcat agtcctggcc gatcttgccc cggcgtttca tcaccgtgct catggtcagc
     9961 atcgaatgcg gggcatcacc ggagaggatg ttggcccggc tggcgccgtc atcggccaac
    10021 aggccgtttc cgtccgactg ccttgcctcc atctcggccg catccttcgg atggttggtc
    10081 acgagggccc ggccggtttc cttctcccac cagcggaagg cgggcatgtc gtggttgttg
    10141 ccgtggagga tcccggcctg gcaggcgccg gtctgggagg accagtcggt ttcccagcct
    10201 tccagcttgt ggctgccatc ccgcacccag gccgccatcg ccggggcgtt gccgttggtc
    10261 atcgcgcgcc ggagaatgtc gtgtgcgagg ccgtcgatct cgaggaagac gatgccgggc
    10321 tcatcggtct cgatcacctg gccgcggcgc ctgagctggg tgcggaccac gttgtagtac
    10381 caggtgctgt cctcgtcgat cgcgaaaagc gaggagaaga gcgaggtcag gatcgccatc
    10441 ccgaaggaga tcacgattgc ttcgaacggt ccctcgatcc gcacccaggg catcgccatc
    10501 agggcgaacc cgatcagcac cgcgttcagg aaaagtcccc agaggccgag ggtcaggacg
    10561 ctgagcttga gggtgaagcg ggaaaggacc ggccagatca aggcgttgac gaggccgacc
    10621 ccgagtgcgg tgggcagggc ggcaaaacgg ccgtgcatgt cgaagcccgg cagaaccgcc
    10681 tcgatcagca aaagcgcggc cgcgtcgatc aggacgacga ccaggattcg ggcgaacagc
    10741 ttgccggggt gccggcgcgt ttccggctcg ttcagtccct ccattgccgg aactctcgca
    10801 aaccgggcgg tccccggcaa tcaccccgac cggatgaacc cctcagcgcc tctttttcgc
    10861 cttcgaaacc ttcctgatca cccgcgtcac gaccttcgac cggttgccgg ccttgtcaat
    10921 cgcccggatc gacaccgtgt tctttccctt gcggagcctg ggggtcttcc agggtgactt
    10981 gcagcttttc cacttgccct tgttcaggcg gcacttgaag gtcgatccct tctcgttcga
    11041 cttgaagcgg accttgaccg tggcggccct ggtctgct
//
LOCUS       DAOQKN010000077        10993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_2805_length_10993_cov_5.484641, whole genome shotgun sequence.
ACCESSION   DAOQKN010000077 DAOQKN010000000
VERSION     DAOQKN010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 10993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10993
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2805_length_10993_cov_5.484641"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="PLJ64_06680"
     CDS             complement(<1..655)
                     /locus_tag="PLJ64_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNC93200.1"
                     /translation="MRLAVLGHPVSHSRSPVMQNAALAAAGLDGWRYEAIDVAPGDFA
                     ELVRSLPGQGFAGVNVTVPHKEAALAIADQASGAAAAIGAANTLTFEDGLIRADNTDA
                     PGLIDALTGRNGDADRDTASGQGKLAGKRALALGAGGAGRAVVWALAGAGMEVMLWNR
                     THSKAEQLAAELGVEVIPPEQHGAIDPGPFAVIVNASAAGLGGGDGLADLPLDPGGFH
                     "
     gene            complement(655..1923)
                     /gene="mltG"
                     /locus_tag="PLJ64_06685"
     CDS             complement(655..1923)
                     /gene="mltG"
                     /locus_tag="PLJ64_06685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNC93201.1"
                     /translation="MSERWFDDLPEHDPNDPEAVERALRRQERATRRQQESGPAKSRK
                     PALGDRGSGSRRGPGGGRRNAGGRSGRSGGPRKGRIVAAAAGAVGLIIVIWFLFALFQ
                     PFKGDGDGEPFPVKVPAGSSVGEIGDLLAGKGIISNATLFEIRATISGKRGDLNHGVH
                     QFRENMSYSAAIDELSKTTSKRTISITIPEGLSRSETAAVVKAAGLPGDYMAATVAVK
                     GFDPDDYGAQGRAENLEGFLFPATYELKPTADVEDLVAEQLQAFKDNIAGVNMKYAKS
                     KNLTVYDVLTIASMIEREVSVPSERKTVAAVIYNRLKMGEPLGIDATIRFALDNWTEP
                     LTDADLETDSPYNTRTNQGLPPGPIGNPGLASIEAAAHPADTDVWLYVVKPGTCGEHV
                     FSSSLAEHNRNVEKYEEAQAAAGGSPTDCG"
     gene            complement(1920..2339)
                     /gene="ruvX"
                     /locus_tag="PLJ64_06690"
     CDS             complement(1920..2339)
                     /gene="ruvX"
                     /locus_tag="PLJ64_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNC93202.1"
                     /translation="MRVLALDYGTARIGCAISDPTGTLATPIAAIEPPEVAEVARVAG
                     EREAAKIVVGLPVSLSGEDSGQTALTRSFADELGDAVEVPVETWDERFTTRMAAQTRR
                     ETGAGSAEDSIAAAHLLESYLQSAAGRSARDPGAAGQ"
     gene            complement(2340..4967)
                     /gene="alaS"
                     /locus_tag="PLJ64_06695"
     CDS             complement(2340..4967)
                     /gene="alaS"
                     /locus_tag="PLJ64_06695"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNC93203.1"
                     /translation="MKSAEIRETYLSYFEERGHRRMASASLVPPPEDTSTLLTVAGMQ
                     PFKPYFLGRETPPASRLTSSQTCFRTPDIEEVGKTLRHLTCFEMLGNFSFGDYFKEEA
                     IPFGWQLSTEGFGFDPEKIWITVFGGDDELGLGPDEEAIAIWKGLGVPDERIVKLPRS
                     ENFWQGGPTGPCGPCSEMYLDRGLDFGSEDDRPGDDSDRFLEYWNHVFMSYDQSEDGK
                     LTPLPKKNIDTGMGLERMASILQGVESVYDTDLFRPLIELAEEKSGLKYGEDEAVTRA
                     MRVISDHSRGMTFLLAAGVVPSNEDRGYVLRRVMRRAIQRGRALGLEGSWLGDFTDRT
                     IEMMGDAHPQVVAERERIDRWVKAEEESFGRTLDRGTALLEDLVDRALTEKNSWISAD
                     DAFKLHDTFGFPYDLTREMVEERGLSVDDQGFEELMEQQRNQSRSNASGGSEGADRHG
                     RILDFAGQGAESEFVGYEYMRTETGIGSLQAVNGSALAKLEQSPFYAEGGGQVADTGR
                     LIWDGGEVEVADVIRVGEDQVLELKPAAEGEAAWPPAGATVEAVVDREARFRTMRNHT
                     ATHLLHAALRERLGSHVHQAGSSVRPDKLRFDFSHGEAMTPEDITWVEERVNGWIKDG
                     DPVRWMIMEKPEAEKLGAMALFGEKYGDLVRVVEVENVSRELCGGTHVDNTAEIGIFK
                     IVHESSSAANVRRIEAITGPDAIDWFRDRASELNEIGHLLGDARDPVRAARDASERLA
                     AASEGARQAARQQQGQLASDLAGQAEEVGGFSVVLAEVPIGNPKEILQIAKQVQGSSG
                     ATAVLAGADPETGKAGMVALFTSDDASKASARDLIAAAAPKIGGGGGGSDEMAQAGGK
                     NPEGVAEALAAAREYLESL"
     gene            complement(4980..6431)
                     /gene="mnmA"
                     /locus_tag="PLJ64_06700"
     CDS             complement(4980..6431)
                     /gene="mnmA"
                     /locus_tag="PLJ64_06700"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNC93204.1"
                     /translation="MESLLAEYVADRSHESEPAPGSFTGAAGGAACGDLARISLVVEN
                     GVVSRACFGTEGCAATRAACACVCELIEGESVLEAARIGAARIEEELGGLSPARRHAA
                     MLAGDALHRALSAVASSDELISPPVEGRVLVAVSGGVDSAVAALGEKEAGAEVVAVTV
                     KLWADPDTDGTKACCSPEAVLGARSLTHDLGIPHFTLDLEGPFRERVVGEFLRGYAEG
                     RTPNPCVLCNGEVRIAAMVDLADRLGAESLVTGHYARVVEDEGGALIAAGSDESKDQS
                     YMLAALPPEVVARLKFPLADLAKDEVREIAERGGLSVARKPESQDLCFLAGQSKKDFL
                     RRHGGLTDQPGPVVDESGSELGRHPGHHHFTVGQRRGLGVAATEPLYVLGTDAKTNTV
                     RVGSRSRLATDRVRIRNAVMHRDGDRVDRVRLRYHTEPVPARVKAGAGSHEALEIELD
                     RPFDGPAPGQAAVLMSGDVVVGHATIASQRGSK"
     gene            6480..7454
                     /locus_tag="PLJ64_06705"
     CDS             6480..7454
                     /locus_tag="PLJ64_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HNC93205.1"
                     /translation="MSKGWNADDITDQTGRTAVVTGGNSGIGLITARELLRHGARVII
                     GCRNEDKGKAAIEQIASDLGTPGIATGIEVRRLDLASLDSVREFAESVDADLPDGLDL
                     LINNAGVMAPPRLLTEDNFELQFGTNHLGHFALTGRLFDTLKRKQGARIVTISSVAAT
                     MGKLDFDDLQSVEGYNRWRAYGQSKLANLVFAMDLQSLIRQAGLDMASIAAHPGVSNT
                     NLTAAGNDLDSGWLGLLSKPFLALSDLLIAQDAAHGALPTLFAATEPGLTGGEYIGPD
                     GLRQYRGSPTIVAPRRLALDSGTGDRLWKASTELTGVEYDFSPASPAA"
     gene            complement(7472..9205)
                     /locus_tag="PLJ64_06710"
     CDS             complement(7472..9205)
                     /locus_tag="PLJ64_06710"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93206.1"
                     /translation="MTSVLRKAGLTGLLAALALAIALPAAASADTQRFYDNWAVGSGP
                     IGIANDRFGDVYVSSNTPAPFLSRYTAEGEFVASFATGGAILGARDVTVDSAGNVYQV
                     NATNNMVRKFSPDGTLITGWGTSGSGDGQFLDPNALATGPDNRIYVGDFGNDRVQWFT
                     PAGVYLGQFATGPMAGLDVGADGNVFVADSLSDEIRVYDPSGTPLDTVGENGSGAGDI
                     DNVSDVDIGDDGRIYAADTSSGMIRVYAPDGSYQGSLGSPGAGDGELNLPNALTADRA
                     GNVWTTQYGGSPRVSLFAYAPRVIGGTNLDFGYSFIGNPPGVQTVLMQNDNYVLPMYV
                     GTASLTSGVDFGLPAPWFECSGVILLPGHVCSVGVDFHPATPGNKTDTLNLDGGWRQV
                     TLSGTGISGPTGPTGATGSTGPTGSTGSSGATGATGSTGATGPTGTGSTGATGATGPT
                     GPKGPTGATGPKGPSGNNATPRVNRIHTVVRVGAGPVAMVSVHCPKASCTIWQRQGRA
                     RSRGRVIQTRVIGPNRIAAGGTARFRVTVPPRIRNLLTRQRSGTVNVYLAVQADKGNS
                     IRRNVRIGIRR"
     gene            complement(9279..9431)
                     /gene="ssrS"
                     /locus_tag="PLJ64_06715"
     ncRNA           complement(9279..9431)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PLJ64_06715"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(9444..9731)
                     /locus_tag="PLJ64_06720"
     CDS             complement(9444..9731)
                     /locus_tag="PLJ64_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93207.1"
                     /translation="MGFLDRFRDRVKSIGDGSGRIQINDPRFDDWETVHQFEDLATGL
                     AWRDVLREHGIPAELTSDWELDRYGRGDIHLQVPPGMWSDAEELVSGLDLE"
     gene            complement(9748..>10993)
                     /locus_tag="PLJ64_06725"
     CDS             complement(9748..>10993)
                     /locus_tag="PLJ64_06725"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93208.1"
                     /translation="ATIAAIATAPGRGGIGVVRVSGDKLADLARELTGKDPVARQASL
                     CSFLAGDGSAIDRGIVLYFPAPNSFTGEDVIEVNERLIRHVLAEMGVEVEIPFERMCY
                     DEAIGRFGSDKPDLRYGYELTDLTEILRGTEFKAFGGVIEGGGQVKGINFGQRELSRA
                     QLDGLIDDAKELGAKGLVWAFREGDGWRAPIAKFLSEGELKNLNEAMGAEEGDLMLVA
                     ADQPAMVAAILGGLRTRLAERWDLIPADAGNRFCWIVDWPLFEFNEEEDRWDALHHPF
                     TAPKGDFDPENPGEARAEAYDLVWNGVEIGGGSIRINQPELQSQIFRALGISDEEAEE
                     RFGFLLEALRYGAPPHGGIAYGVDRFAALLAGVESIRDVIAFPKTASGGDPLTGAPAP
                     IDDVQMRDVGIELRKKKLPGQG"
BASE COUNT         1707 a         3807 c         3561 g         1918 t
ORIGIN      
        1 ggtggaaccc gcccggatca agcggaaggt cggccaggcc gtcccctccc cccagtcctg
       61 cggcggaagc gttgacgatg acggcgaagg ggccggggtc gatcgcgccg tgctgttccg
      121 gaggaatcac ctccaccccc agttcggccg ccagctgctc cgccttcgaa tgggtgcggt
      181 tccagagcat gacctccatc ccggctccgg ccagcgccca gaccaccgcc cggccggcgc
      241 caccggcccc aagggcgagc gccctttttc cggccagttt cccttgaccg gaagcggtgt
      301 ccctgtcggc gtcaccgttg cggccggtca gcgcatcgat caggccaggg gcgtcggtgt
      361 tgtcggcccg gatcaggccg tcttcgaaag tcagggtgtt ggccgccccg atcgccgccg
      421 cggccccgga agcctggtca gcgatcgcca gcgccgcttc cttgtgagga accgtcacgt
      481 tgacacccgc gaacccctgg ccgggcaggc ttcgaaccag ttccgcgaaa tccccggggg
      541 cgacgtcgat cgcctcgtac cgccacccgt ccagaccggc cgcggccagg gcggcattct
      601 gcatcaccgg agagcgcgag tgcgaaaccg ggtggccgag aaccgcgagg cgcatcatcc
      661 gcagtcggtc ggcgagccgc cggccgccgc ctgggcttcc tcgtacttct cgacgttgcg
      721 gttgtgctcg gccaggctcg aggagaagac atgctcgccg caggtgccgg gcttgaccac
      781 atacagccag acatcggtgt cggccggatg cgcggccgct tcgattgaag cgaggccggg
      841 attcccgatc ggccccggcg gcaggccctg gttggtgcgg gtgttgtacg gcgaatccgt
      901 ttccagatcg gcgtcggtca gcggctcggt ccagttgtcg agcgcgaagc ggatcgtcgc
      961 atcgatgccc agcggctcac ccatcttgag ccggttgtag atcaccgccg cgaccgtctt
     1021 gcgctcgctc ggaacggaaa cctcgcgctc gatcatcgag gcgatggtca gcacgtcgta
     1081 gacggtcagg ttcttcgact tcgcgtactt catgttgacc ccggcgatgt tgtccttgaa
     1141 ggcctggagt tgctcggcga cgagatcctc cacgtcggcg gtcggcttca gctcgtaggt
     1201 cgccgggaag aggaaccctt cgaggttttc ggcccggccc tgtgccccgt agtcatccgg
     1261 gtcgaaaccc tttaccgcca cggtggcggc catgtagtcg ccgggcaggc ctgccgcctt
     1321 gaccacggcg gcggtttcag agcgactgag accctcgggg atcgttatcg agatggtccg
     1381 cttcgaggtg gtcttgctta gctcgtcgat cgccgccgag tacgacatgt tctcgcggaa
     1441 ctgatggacc ccgtggttga gatcgccgcg cttgccggag atcgtcgccc ggatctcgaa
     1501 cagggtcgcg ttggagatga ttcccttccc ggcgagcagg tcaccgatct cgccgacgct
     1561 gctgccggcc ggcaccttga ccgggaatgg ttcgccgtca ccgtcgccct tgaacggctg
     1621 gaagagggca aagaggaacc agatgacgat gatcaggccg accgcgccgg ccgcagcggc
     1681 cacgatccgg cccttgcggg ggccgccgga gcggccggag cggccgcctg cgttccttcg
     1741 gccacccccg ggtccgcgac gggagccaga gccccggtcg ccgagcgccg gcttgcggct
     1801 ctttgccggt ccgctttcct gctggcgccg ggttgcgcgc tcctggcgcc gcagggcccg
     1861 ttcgaccgcc tcggggtcgt tcgggtcatg ctcgggaagg tcgtcgaacc agcgctcgct
     1921 cattgcccgg ctgctcccgg atctcgcgcg ctccggccgg ccgctgactg gaggtaactc
     1981 tcaagcaggt gggccgccgc aatcgaatcc tcggccgagc cggccccggt ttcacggcgg
     2041 gtctgtgccg ccatgcgggt ggtgaaccgc tcgtcccagg tttcaaccgg aacctccacg
     2101 gcgtcgccga gttcgtccgc aaagctcctg gtgagcgcgg tctggcccga atcctcgccg
     2161 gaaagtgaaa ccggcagccc gaccacgatc ttcgctgcct cgcgctcccc ggcgactctt
     2221 gcgacctcgg caacttcggg cggctcgatc gccgcgatcg gggtggcgag cgtgccggtc
     2281 gggtcggaaa ttgcgcagcc gatccgggcg gtgccgtagt cgagcgccag gactctcacc
     2341 tagagcgact ccagatactc gcgggcggcg gccagggcct cggctactcc ctcggggttc
     2401 ttgcccccgg cctgggccat ctcgtcgctg cctccgcccc cgcccccgat tttcggcgcg
     2461 gcggccgcga tcaggtcacg ggctgaagcc ttcgaagcat catcggaggt aaagagggcg
     2521 accatgccgg ccttgcccgt ttccgggtcg gccccggcca ggacggccgt cgcccccgag
     2581 gatccctgaa cctgcttggc gatctgaagg atctctttcg ggttgccgat cggcacttcg
     2641 gccagcacca cgctgaagcc gccgacctct tcggcctgcc cggccagatc ggaggcgagc
     2701 tgaccctgct gctggcgcgc cgcctgcctg gctccttcgc tggccgccgc aagtctttcg
     2761 gaggcatcac gagcggcccg gaccgggtcg cgggcgtcac cgagcagatg cccgatttca
     2821 ttcagttcgg aggcacggtc gcggaaccag tcgatcgcat ccggtccggt gatcgcctcg
     2881 atgcggcgca cgttcgcggc cgaggaactc tcgtggacga tcttgaagat gccgatctcg
     2941 gcggtgttgt cgacgtgggt cccgccgcag agctcgcggg agacgttctc gacctcgacc
     3001 acacggacca ggtcgccgta tttctcgccg aacagcgcca tcgcccccag cttctcggcc
     3061 tcgggctttt ccatgatcat ccagcggacc gggtcgccgt ccttgatcca gccgttgacc
     3121 ctttcctcga cccaggtgat gtcctcgggg gtcatcgcct cgccgtggga gaagtcgaag
     3181 cggagtttgt cgggccggac cgaagagccc gcctggtgga cgtggctgcc gagccgttcg
     3241 cgcagggcag cgtgaagcag gtgggtggcc gtgtgattgc gcatggtccg gaatcgggct
     3301 tcccggtcga ccaccgcttc aaccgtcgcc ccggccggcg gccaggcagc ttcgccttcg
     3361 gcggccggct tcagttccag cacctggtct tcaccaaccc ggatcacgtc ggcgacctcg
     3421 acctcgccac cgtcccagat gagccggccg gtgtcggcga cctggccgcc gccctcggcg
     3481 tagaagggac tctgttcgag cttggcgagg gccgagccgt tgaccgcctg aagcgagccg
     3541 attccggttt cggtccgcat gtactcgtag ccgacgaact ccgattcggc gccctggccg
     3601 gcgaagtcga ggatccgccc gtgacgatcc gccccctcgg agccgccgga ggcgttgctg
     3661 cgggactggt tgcgctgctg ttccatcaac tcttcgaagc cctggtcatc aaccgaaagg
     3721 ccccgctcct cgaccatctc gcgggtcagg tcgtagggga agccgaaagt gtcatggagc
     3781 ttgaaggcgt cgtcggcact gatccacgag ttcttctccg tgagggcccg atcgaccaga
     3841 tcctcgagca gggcggtgcc gcggtcgagg gtgcgaccaa agctctcctc ctcggccttg
     3901 acccagcgat cgatccgctc ccgctccgcc accacctgcg ggtgggcgtc acccatcatc
     3961 tcgatcgtgc ggtcggtgaa gtcgcccagc catgagcctt cgagtccgag cgcccggcca
     4021 cgctggatcg cccgccgcat cacccgccga agcacgtacc cgcggtcttc gttcgagggc
     4081 accaccccgg cggcgaggag gaaggtcatg ccgcggctgt ggtcgctgat cacccgcatc
     4141 gcccttgtaa cggcctcgtc ttcgccgtac ttcaaaccgg acttctcctc ggccagttcg
     4201 atcaggggcc ggaagaggtc ggtgtcatag accgactcca caccctgcag gatcgaggcc
     4261 atgcgctcga ggcccatgcc ggtgtcgatg ttcttcttcg gcagcggggt cagtttgccg
     4321 tcctcggact ggtcgtagct catgaagacg tggttccagt actcgaggaa acggtcgctg
     4381 tcatcgccgg gccggtcgtc ctcggaaccg aagtcgaggc cgcggtccag atacatctcg
     4441 gaacaggggc cgcaggggcc ggtcggcccg ccctgccaga agttctcgga gcggggcagt
     4501 ttcacgatcc gttcgtccgg gaccccgaga cccttccaga tcgcgatcgc ctcctcgtcg
     4561 ggtccgagcc cgagctcgtc gtcaccgccg aagaccgtga tccagatctt ttccgggtcg
     4621 aatccgaacc cttcggttga aagctgccag ccgaagggga tcgcctcttc cttgaagtag
     4681 tcgccgaagc tgaagttgcc cagcatctcg aagcaggtga ggtgcctgag ggtcttgccg
     4741 acttcctcga tgtccggggt gcggaaacag gtctggctcg aggtgagccg cgaggcaggc
     4801 ggggtttccc ggccgaggaa atacggcttg aagggctgca tcccggccac ggtcagcagg
     4861 gtcgaagtgt cctccggcgg cggcaccagc gaggccgacg ccatgcgccg atggccccgc
     4921 tcctcgaagt aggacagata tgtctcccgg atttccgccg acttcatgag acccgcagcc
     4981 tactttgatc cgcgctggct ggcgatggtg gcgtggccga cgaccacgtc gccgctcatc
     5041 agcaccgccg cctggccggg tgccggtccg tcgaagggac ggtcgagttc gatttcgagc
     5101 gcctcgtggc tgcccgcccc cgccttcacc ctggccggaa ccggctccgt gtggtacctc
     5161 agccggaccc ggtcgacccg gtcgccgtcg cggtgcatca ccgcattgcg aatccgcacc
     5221 cggtcggtcg ccaggcgact gcgggagcca accctgaccg tgtttgtctt cgcgtccgtg
     5281 ccgagcacgt aaagcggctc ggtcgccgcg acgccgaggc cgcggcgctg gccgaccgtg
     5341 aagtggtgat ggccggggtg ccggccgagt tcggagccgc tttcgtccac gaccgggccc
     5401 ggctggtcgg tcaggccgcc gtgccgccgc aggaagtcct tcttgctctg gccggcgagg
     5461 aagcagaggt cctggctctc cggcttcctg gctaccgaca accctccccg ctcggcgatc
     5521 tcgcggacct cgtccttggc gagatcggcc agcggaaact tcagccgcgc cacgacttcc
     5581 ggcggcaggg cggccagcat gtagctctgg tccttggact cgtcactgcc ggccgcgatc
     5641 agggctccgc cttcgtcctc gacgacccgg gcgtagtgac cggtgaccag gctttcggcg
     5701 ccgagccggt cggccaggtc gaccatcgcc gcgatcctca cctcgccatt gcagagcacg
     5761 caggggttcg gggttctccc ttcggcgtag ccgcgcagga attcgccgac cacccgctcg
     5821 cggaacgggc cttccaggtc gagagtgaag tgcgggatgc cgagatcgtg agtcagagag
     5881 cgggcgccga gcaccgcttc gggcgagcag caggccttgg tgccgtccgt gtccgggtcg
     5941 gcccagagct tcacggtcac cgcgacgact tcggccccgg cctccttctc gccaagtgcg
     6001 gccacggcgc tgtcaacgcc acccgagacc gcgaccagca cccggccctc gaccggtggt
     6061 gaaatcagct cgtccgagga agcgacggcc gaaagcgccc ggtgaagcgc gtcgccggcc
     6121 agcatcgccg cgtgacgccg ggccgggctg agcccgccga gctcctcctc gatccgggcc
     6181 gccccgatcc gggccgcctc cagcacgctt tcaccctcga tcagctcaca aacgcaggca
     6241 caggcggccc tggtcgcggc gcaaccctcg gtcccgaaac aggcgcgcga aaccacgccg
     6301 ttttcgacca ccagggaaat ccgggcgaga tcaccgcagg cggcgccgcc ggcggccccg
     6361 gtgaacgatc cgggggccgg ttccgactca tgcgagcggt cggcgacata ttcggcgagc
     6421 agcgactcca tcccctagag gttaaatggt cgattccccc agcggatacg ctgccccgca
     6481 tgagcaaagg ctggaacgca gatgacatca ccgatcagac gggtcgcacc gcggtcgtca
     6541 ccgggggcaa cagtggcatc gggctgatca ccgcccgtga actgctgcgg cacggagcgc
     6601 gggtgatcat cggctgccga aatgaagaca agggcaaggc ggcgatcgag cagatcgcct
     6661 ccgacctcgg cacgccaggt atcgccaccg ggatcgaagt gcgacggctc gacctcgcct
     6721 ccctggattc ggtccgcgag ttcgccgagt ctgtcgacgc cgacctgccc gacggacttg
     6781 acctcctgat caacaacgcc ggcgtcatgg ctccgccccg gctgctcacg gaagacaatt
     6841 tcgagctcca gttcggcacc aaccacctcg gccacttcgc cctgaccggc cggctgtttg
     6901 acacgctgaa gcgcaaacaa ggggcaagga tcgtgacgat cagttccgtg gcggccacca
     6961 tgggcaagct cgatttcgat gacctgcagt cggtcgaggg ctacaaccgc tggcgggcct
     7021 acggccagtc gaagcttgcc aatctggtct tcgcgatgga cctgcagtcg ctgatccgcc
     7081 aggccggtct cgatatggcg agcatcgccg cccaccccgg tgtctcgaac accaacctga
     7141 ccgcggccgg caatgacctc gactcgggct ggctcggact tctctcaaag ccgttcctcg
     7201 ctctttccga tctgctgatc gcccaggatg ccgcacacgg cgccctgccc accctctttg
     7261 ccgcgaccga gcccggtttg acaggcggcg agtacatcgg cccggacggt ctgcgccagt
     7321 accgcggttc acccaccatc gtggccccgc gcaggctcgc gctcgattcc gggacaggcg
     7381 atcgcctgtg gaaggcgtca accgaactga ccggggtcga atacgacttc tccccggcca
     7441 gtccggctgc ctgaatcccg gcctctcgct gctaccgcct gattccgatc cggacgttcc
     7501 tccggatcga gttgcccttg tcggcctgta ccgccaggta gacgttgacg gttcccgacc
     7561 gctggcgggt cagcaggttc cggatccggg gcggaaccgt gacccggaaa cgggccgtgc
     7621 cacctgcggc gatccggttc ggcccgatta ccctggtctg aatgacgcgg ccgcgggaac
     7681 gggcccggcc ctgtcgctgc cagatcgtgc aggaggcctt cggacaatgg acgctgacca
     7741 tcgctaccgg accggcgccg acccggacga ccgtgtgaat acggttcacc cggggagtgg
     7801 cgttgttgcc acttgggccc ttggggccgg tggctccggt tggccctttc ggcccggtcg
     7861 gtcccgtcgc gccggttgcg ccggtgctac cggttccggt agggccggtt gctccggtcg
     7921 aacccgtggc gccagtcgcg cccgaactgc cggttgagcc agtaggcccg gttgagccgg
     7981 tcgcgccagt aggcccggtt ggcccgctga tcccggtccc gctcagggtg acctgccgcc
     8041 agccgccgtc caggttgagt gtgtcggtct tgtttcccgg tgtggccgga tgaaagtcga
     8101 ccccgacgct gcacacatgg cccgggagca agatcacgcc agagcactcg aaccagggtg
     8161 ccgggagtcc gaagtcgacc ccggatgtca ggctcgccgt cccgacatac atcggcagga
     8221 cgtaattgtc gttctgcatc agcaccgtct ggactccggg agggttaccg atgaaggaat
     8281 acccgaagtc gaggttcgta cctccgatca cccgcggcgc gtatgcgaac aggctgaccc
     8341 ggggcgatcc gccgtactgc gtcgtccaga cattgccggc acggtccgcg gtaagtgcgt
     8401 tgggaaggtt caactctccg tcgccagccc ctggggatcc gagcgagccc tgataggacc
     8461 cgtccggcgc atagaccctg atcatgccgc tggaagtgtc ggcggcgtag atgcgaccgt
     8521 cgtcgccgat gtcgacgtcg ctcacattgt cgatgtcacc cgcgcccgag ccgttttctc
     8581 caacggtgtc gagcggagtt ccggacgggt cgtagacccg gatttcatcg ctcagcgaat
     8641 cggccacgaa tacgtttcca tcggctccga catcgaggcc ggccatgggg cccgtggcga
     8701 attgcccgag gtacacacct gcgggcgtga accactggac tcgatcgttg ccaaagtccc
     8761 ccacatagat cctgttgtcc ggtcccgtgg ccagggcgtt cggatcgaga aactggccgt
     8821 cacccgatcc gctggttccc caccccgtga tcagggttcc gtccggggag aacttcctga
     8881 ccatgttgtt ggtggcattc acctggtaga cgtttccggc cgaatctact gtcacgtccc
     8941 ttgctccgag aatggccccg cccgtcgcaa acgaggcgac gaactcgcct tcggccgtgt
     9001 atcgggagag aaacggcgcc ggagtgttgc tggaaacgta gacatccccg aaccggtcgt
     9061 ttgcgatccc gatcggaccc gacccgactg cccagttgtc gtagaagcgc tgggtgtcag
     9121 ccgaagctgc tgccggcaaa gcgattgcca gtgcaagggc ggccagcaat ccggtcagcc
     9181 ctgccttccg tagaaccgag gtcatttccc ctcctcttct tgatgtctgc cgtccaccct
     9241 attcgcggtg gcggtcaagt tcaggcgaag atagaaaaag aggctccaac ctgccgttgc
     9301 cgcctccggc attgatccct ttataaaaag gtgggtgcct atcttcgtcc cgcgggggac
     9361 atcgaaatca gctcccgtgc cgttgatgtt gggtcaattt acgagacggc tgtcgaacag
     9421 gttcgagcct caaccccaac ctatcactcc aggtcaagcc cggacacgag ttcttcggca
     9481 tcggaccaca ttccgggtgg cacctggagg tggatgtctc cccggccgta gcgatcgagc
     9541 tcccagtcgg aggtcagctc ggccgggatg ccgtgttcgc gcaggacatc ccgccaggcc
     9601 aggcccgtgg ccagatcctc gaactggtgg acggtctccc agtcgtcgaa acgagggtcg
     9661 ttgatctgga tccggcccga gccatccccg atcgacttca ccctgtccct gaagcgatcg
     9721 agaaaaccca tcgcgcgacc ccgctgccta accctgcccg ggaagtttct tcttgcgaag
     9781 ctcgatcccg acgtcgcgca tctggacgtc gtcgatcggg gccggagcgc cggtcagcgg
     9841 atcgccgccg gaagcggtct tggggaaggc gatcacgtca cggatcgatt cgaccccggc
     9901 cagcagcgcc gcgaagcgat cgaccccgta ggcgatgccg ccgtgaggcg gtgccccgta
     9961 tcggagggcc tcgagcagaa agccgaatcg ttcctcggct tcctcatcgg agatacccag
    10021 ggccctgaag atctgtgact ggagttccgg ctggttgatg cggatcgaac cgccgccgat
    10081 ctcgaccccg ttccagacca ggtcataggc ctcggccctg gcctcgcccg ggttttccgg
    10141 atcgaaatcg cccttcggcg cggtgaaggg atggtgcagg gcatcccagc ggtcttcttc
    10201 ctcgttgaat tcgaaaagcg gccagtcgac gatccagcag aaccggttgc cggcatcggc
    10261 cgggatcagg tcccagcgct cggcaagccg ggtgcgaagg ccgccgagga tggccgcgac
    10321 catggccggc tggtcggcgg cgaccagcat caggtcgcct tcttcggcac ccatcgcctc
    10381 gttcaggttc ttcagttcgc cttcagagag gaacttcgcg atcggggccc gccagccgtc
    10441 gccctcacgg aaggcccaga ccaggccctt ggccccgagc tccttggcgt cgtcgatcag
    10501 gccgtcgagc tgggcccggg aaagctcgcg ctggccgaag ttgatgccct tgacctgccc
    10561 tccgccttcg atcacgccgc cgaaggcctt gaattcggtg ccgcggagga tttcggtcag
    10621 gtcggtcagt tcgtagccat agcgaaggtc cggtttgtcg gagccgaagc ggccgattgc
    10681 ctcgtcgtaa cacatgcgct cgaaggggat ctccacttcg acccccatct cggccagcac
    10741 atgccggatc aggcgctcgt tgacctcgat cacgtcttcg ccggtaaagg aatttggcgc
    10801 cggaaagtaa agcacgatgc cgcgatcgat cgcgctgccg tcgccagcca ggaaggaaca
    10861 caggctggcc tgccgggcca ccgggtcctt cccggtcaac tcccgcgcca gatccgccag
    10921 cttgtcgcct gaaacgcgaa caaccccgat gccgccgcgc ccgggcgccg tcgcgatggc
    10981 cgcgatcgtc gcc
//
LOCUS       DAOQKN010000078        10947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_2818_length_10947_cov_6.289570, whole genome shotgun sequence.
ACCESSION   DAOQKN010000078 DAOQKN010000000
VERSION     DAOQKN010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 10947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10947
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2818_length_10947_cov_6.289570"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..512)
                     /locus_tag="PLJ64_06730"
     CDS             complement(90..512)
                     /locus_tag="PLJ64_06730"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNC93209.1"
                     /translation="MSKTAREVMTGGAECIGESESITRAATRMAELDVGSMPICGEDD
                     RLKGMLTDRDIVVKVIAEGKDPDEVTAGEFGQGKPVTVGADDSVEVAIETMKEHAVRR
                     LPVIDGHELIGIVSQGDLACEVSEKAAGDLVEAISSAP"
     gene            694..909
                     /locus_tag="PLJ64_06735"
     CDS             694..909
                     /locus_tag="PLJ64_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93210.1"
                     /translation="MVKVGIAIGLVFLVAALSVFGSPFFAVLLLVPAGILAFAFLGGA
                     VVAEETGPEDSGKEPGPPKTSEAGQQN"
     gene            982..1692
                     /locus_tag="PLJ64_06740"
     CDS             982..1692
                     /locus_tag="PLJ64_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93211.1"
                     /translation="MIEAIGWGAVAASSLVLGVLLAFARPWSDRLVGSVLAFGAGALI
                     SAVSFSLAGEGLDIGSPPSVGAGLAVGALAYYLASRFIDRPRPGERADSSASGRDLAL
                     GSFLDGIPEQLVLGIGIVSGSGISLALLMAIFVSNLPEGIGSAADMERAGASRAYVLR
                     LFILIAIACAIAAAVGYLLADFISNELQACLDGFAAGALLVMMIDSMIPEAREKAGNV
                     AGLLTVVGFAAATALSGA"
     gene            complement(1695..2156)
                     /locus_tag="PLJ64_06745"
     CDS             complement(1695..2156)
                     /locus_tag="PLJ64_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93212.1"
                     /translation="MKFRETAIKALVLIGAVATCLAFTVPATSARSSKPPVFWIDLSG
                     TAQQHPDFVFFSANAGSQVNHLKWTGWGKNRAVGRGHYYVTSPPIPGEKNPEGAARIV
                     VWKPVKCVPDFGNREGKTIHIYKHARMLRPVSGGGRKWIDISAFAGAATCR"
     gene            complement(2257..2559)
                     /locus_tag="PLJ64_06750"
     CDS             complement(2257..2559)
                     /locus_tag="PLJ64_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002530052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HNC93213.1"
                     /translation="MADSNGNTHGYVHDKERYLARLKRIEGQARGIHRMIEEDKYCID
                     ILTQISALTSALEGVAIGLLDEHMKHCVVDAAREGGPEAEAKIKEASDAIARLVRS"
     gene            complement(2549..4912)
                     /locus_tag="PLJ64_06755"
     CDS             complement(2549..4912)
                     /locus_tag="PLJ64_06755"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNC93214.1"
                     /translation="MSATRTETGESPDPITRRELDFEISGMTCASCAARIERNLNRLD
                     GIDASVNYATEKARVKVPEGLAPEEIIAEVEKTGYSAALPPDSNQDGTGRDHAGHSGG
                     HDHGGIAGPETDREIEVLRQRLIGSILLAVPVIAMSMFEALQFEYWQWASLVLAAPVV
                     VWAAWPFHRATLMNLRHGATTMDTLVTIGISAAFFWSLYALFFGTAGEPGMTHPFEIT
                     VAPSDGASNIYLEVAAGVTMFLIAGRYFELRSKRRASSAMNALLEMGAKEAFVIRDGE
                     EVAVAIGDLAVGDEFVVRPGEKVATDGVVVSGNSAIDESMLTGEPVPKEVGAGDEVAG
                     ATVNAGGRLTVRATRVGADTQLARMAKMVEDAQSGKAPVQRLADRISAYFVPAVILIA
                     AATLGAWLGAGFPADAAFTAAVAVLIIACPCALGLATPTALLVGTGRGARMGILIKGP
                     EVLESTRQIDAVVLDKTGTVTEGRMELVEVITAEGVDRRELLAIAGALENSSEHPIAK
                     AIARVAAEETGKLPQPEGFASVAGRGVQGVVDGHAVLVGRESLLNDWSQHLPPDLAEA
                     KSSREGEGRTVVIASWDGAPRGLVVVADTVKPTSPEAVSELLELGLEPVLLTGDNEAA
                     ARAVAKEVGIDRVIAEVLPGEKVEVIKRLQGEGKVVAMVGDGVNDAPALAQADLGLAM
                     GTGTDVAIEAADITLVRGDLRSVGEAIRLSRRTLGTIKVNLFWAFAYNVAAIPLAAFG
                     LLNPMLAGAAMAFSSVFVVGNSLRLRSFGRGREEPAGNIEKGTGNGR"
     gene            complement(4909..5832)
                     /locus_tag="PLJ64_06760"
     CDS             complement(4909..5832)
                     /locus_tag="PLJ64_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93215.1"
                     /translation="MSTPLRLLLYGLSVLAVFAVSFVAGKALIPEDTVSDWTRQADAS
                     SAGHESMAGDHDEAHVGGLSVEQDGYTISPVSSPSVAGQPGELSFRILDPEGKPLTGY
                     SSNHEKQLHLITVRLDGDRFRHVHPRLDLASGTWSIPWSWPAGGSYRVFADFVPQGSS
                     PDQALTLSRSVEVGGEFMPEPKPLSRTDSTGGFKATLRGELEAGSSSELTAHLTRGGR
                     PVTDLEPYLGAFGHLVALREGDLGYLHVHAEDGTEGNPVQGHEHAHSPETAGPDIEFA
                     AEAPTPGRYLLYLDFKVDGRVHTAEFVLEAK"
     gene            complement(5865..6443)
                     /locus_tag="PLJ64_06765"
     CDS             complement(5865..6443)
                     /locus_tag="PLJ64_06765"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNC93216.1"
                     /translation="MSRDFRELPPDLPVPVDDGAADHLEGMAFPELSLRSTSGGEVSL
                     PSAFGERAVLFTYPRTGTPGEPLLPGWDDIPGARGCTPQSCAYRENLAGFRSAGLAVF
                     GLSAQTAEAHAEFHARESIPYELISDPGLKLAETLRLPTFAISSLTLYRRLTMIIEAG
                     QIAKVFYPVFPPDQDAATVLAWLAGDDLAETS"
     gene            complement(6440..6649)
                     /locus_tag="PLJ64_06770"
     CDS             complement(6440..6649)
                     /locus_tag="PLJ64_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93217.1"
                     /translation="MAEEPVSKNKDGSRIGSDEYVTLALCIIVPIIGLLFALYYRQQG
                     EKWAGRAMVVGVVALVVWLCLVFLI"
     gene            complement(6689..7381)
                     /locus_tag="PLJ64_06775"
     CDS             complement(6689..7381)
                     /locus_tag="PLJ64_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNC93218.1"
                     /translation="MDSESQALPTLRQQGDVYLLDLGDGENRFNPDRNSVIEELLAEV
                     EAAPAPRALVTVASGKVWSNGLDLEWFQAHPDRVQEALDQTERLFAAFLGAGIPTVAA
                     IQGHCFAGGAMLALAHDYLIMREDRGYLCLPEADLGLPFTPGMNSLLLDRLPRRTAHE
                     VMVTGRRYGGTDAAAAGIVDQVASEDLLVPAAIERAAGQAPKNPETLRAIKRRIYERT
                     IELLETSHPLGS"
     gene            7461..8141
                     /locus_tag="PLJ64_06780"
     CDS             7461..8141
                     /locus_tag="PLJ64_06780"
                     /inference="COORDINATES: protein motif:HMM:NF022589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAP family protein"
                     /protein_id="HNC93219.1"
                     /translation="MGEAVGQTLPLAVGVAISPIGIIASVVMLTSGRGAGNGLAFLVG
                     WFIGLAVACWVLLQLNLGADPASGGHPADWTSYAKIILGLLLLVVAHRQRGKKTAPGE
                     TPELPKWMDKADSIKPLRATGLAFLLGAVNPKNLILTMAAATAIGQVDAGSADQLTSV
                     LVYALIASVGAAVPVGIYFLMGDRADSMLARLKESMARNNGVIMAVICVVIAAKLIGD
                     GITTLSLF"
     gene            complement(8161..8844)
                     /locus_tag="PLJ64_06785"
     CDS             complement(8161..8844)
                     /locus_tag="PLJ64_06785"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNC93220.1"
                     /translation="MFDSDFDPRDRGRFEGRCGNPGFGPAAGDGQPGFGPETDSGRRR
                     YRHGPGRGGPHGFGGGHHGRGPGRPGRGRRARRGDIRQAILLLLEEEPRNGYGLMQEV
                     EERSGGAWRPSPGSVYPALSQLEDEGLVEMAESEGRKDFSLTGAGREWVEEHREEIGS
                     PWDTEGRGTPRELGSLRKSTQALAMAARQLAHTGTPAQLEEARKLVDEARRKLYGILA
                     QSEEEQEQS"
     gene            complement(8949..10889)
                     /locus_tag="PLJ64_06790"
     CDS             complement(8949..10889)
                     /locus_tag="PLJ64_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93221.1"
                     /translation="MAAIVSLPLALALLTLSSAGIAPADAAGAGSVAARELAEKYVPV
                     MMIRKQGENLCDTKVEQYQVMTVDALFGNPAVKLMRNNGKQDSSLITRAPTLRDIRNR
                     GEEAYIDLPGDPLGETCVYAKDFDRMRREGRAPVAVYAHIAREKGHSGLALQYWFYWY
                     FNQFNDLHESDWEGMQITFDANTPAGALKQEPHEMIVFQHAGGERANWDDAKVEKEGS
                     HPVVYPAAGSHATFYQSAIYPQNGSHGSGVGCDNTSEPLTELRPVPVLLPERPTDRGE
                     FAWLSFDGRWGQKEKSFNNGPTGPQTKTQWDEPFTWMEAQRWSSPRMPGGGLVGPEAV
                     NAFCGVIESVTGVMNLQQSDPLAAYLIAAAFILIVFFVFGYSKWRPAGADRLEQKRAY
                     GQIMNASFRLYWRHLSAFAVLGAIAIPIVGGTQALGGWLGDMSGGDGLLQTLSDLVNS
                     FGRPAAQALISAFLIVFIRDLVQGQTATVRTALDGTRERFWRVVIAKMLAIAGVTLLA
                     LTVIGIPFAVRFLVNWTFVQQEVIFTDLSIRDSFRASSDLVRGHWWRTVRTMVPLLLL
                     LTVLGPLLGLFLIFTPLPLLLVNLIGSVVFAIAIPFTTAGTTLLYFDLRARQQEHGVV
                     PRRSWAVWRPATFGRPVGQPTA"
BASE COUNT         1771 a         3667 c         3538 g         1971 t
ORIGIN      
        1 tcgagctaaa gcagaagctg cccggctgac ccgggagagg gagtcaagga ttggggcgcc
       61 gcccttgcca cggcgacgcc ccgggcgggt cagggtgcgg aggaaatcgc ttccacgaga
      121 tccccggccg ccttctcgga gacctcacag gcgaggtcac cctgactcac gattccgatc
      181 agttcatgtc cgtcgatcac cgggagccgc cttaccgcgt gctccttcat ggtctcgatc
      241 gcgacttcaa ccgagtcgtc ggctccgacg gtgacgggct taccctgacc aaactcgccg
      301 gcggtcacct cgtcgggatc ctttccttcc gcgatcacct tcacgacgat gtcgcggtcg
      361 gtcagcattc ccttcaggcg gtcgtcttcg ccgcaaatcg gcatcgatcc cacatcaagt
      421 tcggccatcc gcgtggcggc gcgagtgatg ctctccgact cgccgatgca ttcggcaccg
      481 ccggtcatta cttcccgtgc tgtcttgctc atctcctgcc tcctgtctgg gtggtttgcc
      541 cctccgccga ccgctgtggc ggagttcaac tagaggcgta cccccagccg gagcgtccaa
      601 actcaaagat tgggagatcc ggtgtcgggt acctgccgaa gcgttcccga aagttggccg
      661 gtcgcgattt cgcggcgggc cggaaaggcg tcgatggtca aagttggcat cgctattgga
      721 ctcgttttcc tcgtcgcggc tctctccgta ttcggatcgc cgttcttcgc agtcctgctt
      781 ctggtcccgg ccggcattct cgcctttgcc ttcctgggcg gagcggtagt ggccgaggag
      841 accggtcccg aagattccgg gaaagagccg ggtccgccga agacctccga ggcaggccag
      901 cagaactgac cccgggcccg ggttcggtat ccgccgcacc cgtctagagt ccccgccgat
      961 ggccgtggcg gagactttcc gatgatcgag gcgatcggct ggggtgccgt tgccgcgtct
     1021 tctctggttc tcggcgttct gctcgcgttc gcgcggccct ggtccgaccg cctggtcggg
     1081 tccgtgcttg cctttggcgc tggtgcgctg atcagcgcgg tcagtttcag cctggccggc
     1141 gagggcctgg acatcggcag cccgccatcg gtcggcgccg gtctcgcggt tggagcgctt
     1201 gcctactacc ttgcgagccg tttcatcgac cggccccggc ccggcgaaag ggccgattcg
     1261 tcagccagcg gccgggacct tgccctcggg tccttcctcg acgggattcc ggaacagctg
     1321 gtgctgggta tcggcatcgt ttccggatcc gggatcagcc tggccctgct gatggcgatc
     1381 ttcgtttcaa acctgccgga gggaatcggt tccgccgccg acatggagcg ggcgggtgcc
     1441 tcgcgggcgt acgtactccg gctgttcatc ctgatcgcga ttgcctgcgc gatcgctgcg
     1501 gcggtcggtt acctgctcgc cgatttcatc tcgaacgaac tccaggcctg cctggatggt
     1561 ttcgccgccg gcgccctgct ggtgatgatg atcgactcga tgattcccga agcaagagaa
     1621 aaggccggca atgttgccgg ccttctcacg gttgtcggtt ttgccgcagc gactgcgctg
     1681 agtggcgcct gaacctacct gcaggtggcc gcgccggcga aggcggagat gtcgatccac
     1741 ttgcgccctc ccccggaaac gggtcgcagc atcctcgcgt gcttatagat gtgaatcgtc
     1801 ttgccctcgc gattgccgaa atccgggacg cacttgaccg gcttccagac gacgatccgc
     1861 gccgcgcctt ccgggttctt ttcaccgggg atcggcggcg aggtcacgta gtagtggccg
     1921 cggccgaccg cgcggttctt gccccagccg gtccacttga ggtgattgac ctgggagccg
     1981 gcattcgccg agaagaaaac gaagtcggga tgctgctggg cggttccgga gaggtcgatc
     2041 cagaagaccg gaggcttgga ggacctggcc gaggtggccg gaacggtaaa cgcgagacag
     2101 gttgcgacgg caccgatcag caccagcgcc ttgattgcgg tctccctgaa cttcattgaa
     2161 taccccctgt tgattggtcg gagcccgtga tgatatccgc tgctgcgccc ggcctcaatt
     2221 tcgaagcagt gaggcaaggg ccggtcgcga ccgatttcac gacctgacca ggcgggcgat
     2281 cgcgtcggag gcttccttga tcttcgcctc ggcttccggg cctccctccc tcgccgcgtc
     2341 aaccacgcag tgcttcatgt gctcgtcgag caggccgatc gccactcctt ccagggccga
     2401 ggtaagcgcc gagatctggg tgaggatgtc gatgcagtac ttgtcctcct cgatcatccg
     2461 gtggatgccg cgagcctggc cttcgattcg tttcaggcga gccagatacc gctccttgtc
     2521 gtgaacgtac ccgtgggtgt ttccgttgct atcggccatt gcccgttcct ttctcgatgt
     2581 tgccggccgg ttcctccctg ccccgcccga acgatctcag ccgcaggctg ttacccacca
     2641 cgaagacgct ggagaaggcc atcgccgccc cggccagcat cgggttgagc aggccgaagg
     2701 ccgccagcgg gattgccgcc acgttgtagg cgaaagccca gaagaggttc accttgatcg
     2761 tgccgagagt acgccgggaa agccggatcg cctcgcccac gctcctcaga tccccacgga
     2821 ccagggttat gtcagccgcc tctatcgcca cgtcggtgcc ggtgcccatc gcgagcccga
     2881 ggtcggcctg ggcgagcgcg ggcgcgtcgt tgacgccgtc gccgaccatc gcgacgactt
     2941 tgccttcacc ctgaaggcgc ttgatcactt cgaccttctc gccgggcaga acctcggcga
     3001 tcacccggtc gatcccgacc tccttcgcca ctgcgcgggc cgcggcctcg ttgtcaccgg
     3061 tcagcagcac cggttcaagg ccgagttcga gaagttcact caccgcttcg gggctggtcg
     3121 gcttgaccgt gtcggctacg accacaagtc cgcgtggtgc gccgtcccag ctggcgatca
     3181 caacggtccg gccctcgcct tccctcgatg actttgcttc ggccaggtcc ggcgggagat
     3241 gctgggacca gtcgttgagc agcgattcgc gaccgaccag cacggcgtga ccgtcgacca
     3301 cgccctggac tccccgtccg gcgacgctgg cgaagccctc cggctgcggc agctttcccg
     3361 tctcttcagc tgccacccgg gcgatcgcct tcgcgatcgg gtgctcggag gagttctcga
     3421 gggctccggc gatcgccagc agttcgcggc ggtcgacccc ttcggccgtg atgacctcga
     3481 ccaattccat tcgtccttcg gtcacggttc cggtcttgtc caggacgacg gcgtcgatct
     3541 gcctggtcga ttcgagaacc tccggcccct tgatcaggat gcccatgcgg gctccgcggc
     3601 cggtgccgac cagaagtgca gtcggggtgg ccagaccaag ggcgcagggg caggcgatga
     3661 tcagcaccgc gaccgccgcc gtgaacgcgg cgtcagcggg gaaaccggcc ccgagccagg
     3721 ctcccagcgt agccgccgcg atcaggatca ccgccggcac gaagtaggcc gagatccggt
     3781 cggccagtcg ctgcaccggt gccttgcccg actgggcatc ctcgaccatc ttcgccatgc
     3841 gggccagctg ggtgtcggcg ccgaccctgg tcgccctgac cgtcagtcgg ccgcccgcgt
     3901 ttacggtcgc tccggcgact tcatcgccgg cgccaacctc tttcgggacg ggctcgccgg
     3961 tcagcatcga ctcgtcgatc gccgagttgc ccgacaccac cacaccgtcc gtcgccacct
     4021 tctcgccggg acgaaccacg aactcgtcac cgaccgccag gtccccgatc gcgaccgcaa
     4081 cttcctcccc gtccctgatc acgaaggctt ccttcgcgcc catctcgagc agggcgttca
     4141 tggccgagct cgccctccgc ttcgagcgca gctcgaagta gcgtccggcg atcaggaaca
     4201 tggtcacgcc cgccgcgacc tcgaggtaga tgttcgaggc gccgtcggaa ggagcgacgg
     4261 tgatctcgaa gggatgggtc atgcccggct cgcccgcggt accgaagaag agcgcgtaga
     4321 gcgaccagaa gaaagcggcg ctgattccga tcgtcaccag ggtgtccatg gtggtcgccc
     4381 cgtggcgcag gttcatgagg gtggcccggt gaaacggcca ggccgcccag acgacgacgg
     4441 gcgcggcgag gacgagcgag gcccactgcc agtattcgaa ctgaagcgcc tcgaacatcg
     4501 acatcgcgat caccgggact gccagcagga tcgagccaat cagcctttgg cggaggacct
     4561 cgatttcgcg atcggtttcc ggtcccgcga taccgccgtg atcgtgaccg ccggaatgac
     4621 cggcatggtc acgcccggtc ccgtcctgat tggaatccgg cggcagtgcg gccgagtagc
     4681 cggtcttctc cacctcggcg atgatttctt cgggcgccag gccctcgggc actttcaccc
     4741 gggccttctc ggtcgcgtag ttgacgctgg cgtcgattcc gtccagcctg ttcaggttcc
     4801 tctcgatgcg cgcggcgcag gaggcgcagg tcatcccgct gatctcgaaa tctagttccc
     4861 gccgggtgat cgggtcgggg ctttcgccgg tctctgtccg cgtggcactc attttgcctc
     4921 cagtacgaat tccgccgtgt gtacccggcc atccaccttg aaatcgaggt agagcaggta
     4981 acggccggga gtcggcgctt cggccgcgaa ctcgatgtcg ggtccggcgg tttccgggct
     5041 gtgggcatgt tcgtggccct gaaccggatt gccctcggtc ccatcttccg cgtgaacatg
     5101 caggtaaccg aggtcacctt cgcgcagggc aaccaggtga ccgaaggcgc cgaggtacgg
     5161 ctccagatcg gttaccggcc ggccgccgcg ggtcaggtgt gcggtcaact cgctgctcga
     5221 gccggcttcg agctcgccgc gaagggttgc cttgaagccc ccggtcgagt cggtccggct
     5281 gagcggcttc ggctcgggca tgaattcgcc gcccacttcg acgctgcggc tcagggtcag
     5341 agcctgatcc ggggaggatc cctgcggcac gaagtcggcg aagacccggt aggaccctcc
     5401 ggccggccat gaccagggga tcgaccacgt gccggaggcc aggtcgagtc tggggtgcac
     5461 gtgcctgaac cggtcaccgt caaggcgaac cgtaatcagg tgaagctgct tctcgtgatt
     5521 gctcgagtat ccggtcaggg gtttcccttc cgggtccagg atccggaagg agagctcacc
     5581 cggctggccg gccacagagg gggaactgac cggggaaatc gtgtagccgt cctgctcgac
     5641 cgagagcccg ccgacgtggg cttcgtcgtg gtcccccgcc atggattcat gcccggccga
     5701 gctggcatcg gcctgccggg tccagtcgga aaccgtgtct tccggaatca gggccttgcc
     5761 ggcgacgaag gaaactgcga atacggccag gaccgaaagc ccgtagagaa gaagtcgaag
     5821 cggggtgctc atgtcgaaca ccctataccc ctatggggta tatgtcaaga ggtctccgcc
     5881 aggtcgtccc cggccagcca ggcgagcacc gtggcagcgt cctgatccgg cgggaagacg
     5941 ggataaaaga ccttcgcaat ttgtcccgct tcgatgatca tggtcagccg ccggtacagg
     6001 gtgaggctgg aaatggcaaa ggtcggcagc ctcagcgtct cggccagttt cagtccggga
     6061 tcactgatca gctcgtaggg aatcgattcc cgcgcgtgga actcggcatg ggcttcggcg
     6121 gtctgggcac tcaacccgaa gacggccagc ccggccgacc ggaaaccggc caggttttca
     6181 cggtaggcgc aggactgagg cgtacaaccc ctggcgccgg gaatgtcatc ccagcccggc
     6241 agaagcggct cccccggggt gccggtccgc gggtaagtga agagaacggc ccgctcgccg
     6301 aaggccgagg gcaggctcac ttccccgccg ctcgtggacc tcaggctgag ctccgggaac
     6361 gccatcccct cgaggtggtc cgcggccccg tcatcgacgg gaaccggcag gtcgggcggg
     6421 agctctctga agtcgcggct caaatcagga agaccaggca gagccagacg accagggcga
     6481 cgacgccgac gaccatcgct cggccggccc atttctcgcc ctgctggcgg tagtagaggg
     6541 cgaagagcaa cccgatgatc ggcacgatga tgcagagcgc cagcgtgacg tattcatcgg
     6601 agccgatcct gctgccgtcc ttgttcttgc ttaccggttc ttcggccatg ggcgaaccct
     6661 acccgggcag atcgcgcggg cgtcccggtc aggacccgag gggatgcgaa gtttcgagca
     6721 gctcgatcgt tcgctcgtag atccggcgtt tgatcgccct gagggtttcc gggttcttcg
     6781 gtgcctggcc ggccgccctt tcgatcgccg ccgggaccag cagatcctcg gaagccacct
     6841 ggtcgacgat tccggcagcg gcagcgtcgg tcccgccgta ccgcctcccc gtgaccatca
     6901 cctcgtgcgc ggtccggcgg ggaaggcggt cgagcagcag cgagttcatg cccggagtga
     6961 aaggcaggcc aaggtcggct tcgggcaggc atagataccc ccggtcttcc cgcatgatca
     7021 ggtagtcgtg ggcgagagca agcatcgccc cgcccgcgaa gcagtggccc tggatcgctg
     7081 ccacggtcgg gatcccggca ccgaggaagg cggcgaacag tctctcggtc tgatcgaggg
     7141 cttcctggac ccgatccggg tgcgcctgga accactcgag atcgaggccg ttggaccaga
     7201 cctttccact cgccacggtg accagcgccc gcggcgcagg tgccgcttcg acctccgcga
     7261 gcagctcctc gatcacggag ttccgatccg ggttgaaccg attctctccg tcgccgagat
     7321 cgagcagata aacgtcgccc tgttgcctca gggtcggcaa agcctgactc tctgaatcca
     7381 tcgcgctccc ttcttgctgg tttccgccga gcctacaggc gaaagccgcg agctccgatc
     7441 gggcgggtta tcctgtggcc gtgggagagg cggttggaca gacacttccc ctggccgtcg
     7501 gcgtggcgat cagcccgatc ggcatcatcg cctcggtggt gatgctgact tccggccgcg
     7561 gcgccggcaa cggtctcgca ttcctggtcg gctggtttat cggcctggcg gtcgcctgct
     7621 gggtcctgct ccagctcaac ctgggggccg accctgcctc ggggggccat cccgccgact
     7681 ggaccagcta cgcgaagatc attctcggcc tgttgctgct ggtcgtcgcc catcgccaga
     7741 gagggaagaa aacggctcca ggcgaaaccc cggagctgcc gaagtggatg gacaaggccg
     7801 attcgatcaa gccgcttcgt gcgaccggcc tcgcctttct gctcggcgcg gtcaatccca
     7861 agaacctgat cctgaccatg gccgccgcta ccgcgatcgg gcaggtggac gccggtagtg
     7921 ccgaccagct gacttcggta ctggtctacg ccttgatcgc cagcgtcggg gcggccgtcc
     7981 cggtcgggat ctacttcctg atgggtgacc gggccgattc gatgctggcc aggctcaagg
     8041 agagcatggc ccgcaacaac ggcgtgatca tggccgtgat ctgcgtggtg atcgccgcca
     8101 aactgatcgg cgacggaatc accacgcttt cactcttcta gatcgccggc ccgcggaagc
     8161 tcaggactgc tcctgctcct cttcgctctg ggcgaggatc ccgtagagct ttcgccgggc
     8221 ttcatcgacg agctttcgcg cttcctcgag ctgggccggt gtgccggtat gggccagttg
     8281 cctggcggcc atcgccagcg cctgggtcga cttcctcagg gaaccgagtt cccggggagt
     8341 cccgcggcct tcggtatccc agggagaacc gatctcctcg cgatgctcct cgacccattc
     8401 tctgccggcg ccggtcaggg agaagtcctt gcggccttcg gattcggcca tctcgaccag
     8461 gccttcgtcc tcgagctggg agagcgccgg gtagaccgag ccggggctcg gacgccaggc
     8521 accgccgctt cgctcttcga cctcctgcat caggccgtag ccattgcggg gctcctcttc
     8581 gagcaacagc aggatcgcct gccggatgtc acctcgccgg gccctcctgc cgcgtccggg
     8641 gcgaccaggc ccccggccat ggtggccgcc gccgaaaccg tggggtccgc cccttcccgg
     8701 cccgtgccgg tagcggcgcc ggcccgaatc cgtctccggg ccgaaacccg gctggccgtc
     8761 accggccgcg ggcccgaagc cgggattgcc gcagcgaccc tcgaatcgcc cgcggtcgcg
     8821 cgggtcgaaa tctgaatcaa acatctgaaa aacctttcgt ttcgaatatc tcacgataca
     8881 taacgatata tcgtgatact caaaatgtca agaggcggta ccgccggttg gatgccccgg
     8941 cgcaggcttc aggcggtggg ctgcccgacc ggccggccga aagtcgccgg tcgccagacg
     9001 gcccaggagc ggcgcggcac caccccgtgt tcctgctggc gggctcgcag atcgaagtag
     9061 agcagggtcg tgccggcggt cgtgaacgga atcgcgatcg caaagaccac cgatccgatc
     9121 aggttgacca gaagcagcgg caagggagtg aagatcagaa agagaccaag cagcggtccc
     9181 agcacggtca gcagcaagag cagcgggacc atggtccgga ccgttcgcca ccagtgcccg
     9241 cggaccagat cgctgctggc ccggaaggag tcacggatcg acaggtcggt gaagatcacc
     9301 tcctgctgga cgaaggtcca gttgacgagg aagcggaccg cgaagggaat tccgatcacg
     9361 gtcagagcaa gcagggtcac tccggcaatc gcgagcatct ttgcgatcac gacgcgccag
     9421 aaccgttccc gggtgccgtc gagcgccgtc ctcacggtcg cggtctgccc ctgaaccagg
     9481 tcacggatga acacgatcag aaaggccgag atcagcgcct gggccgccgg ccggccaaaa
     9541 ctgttgacca ggtcggagag cgtctgcagc aacccgtccc cgccggacat gtcccccagc
     9601 cacccgccga gggcctgggt gccgccaacg atcgggatcg cgatcgcccc cagcaccgca
     9661 aaggccgaca ggtggcgcca gtacagcctg aagctcgcgt tcatgatctg tccgtaggcg
     9721 cgcttttgct caaggcgatc ggcacccgcg ggacgccatt tcgagtaccc gaacacgaag
     9781 aagacgatca ggatgaaagc cgcggctatc aggtaggccg cgagcggatc ggactgctgc
     9841 agattcatca cgccggtcac gctctcgatc accccgcaga aggcgttcac cgcttccgga
     9901 ccgaccagcc ctccgccggg catccggggg ctcgaccagc gctgcgcttc catccaggtg
     9961 aagggctcat cccactgggt cttggtctgc gggccggtcg ggccgttgtt gaagctcttc
    10021 tccttctggc cccagcgccc gtcaaaggaa agccacgcga attcaccgcg gtcggtgggc
    10081 cgctccggca agagcaccgg gaccggcctg agctcggtca gcggctccga ggtgttgtcg
    10141 cagcccactc ccgatccatg cgagccgttc tggggataga tcgccgactg gtagaaggtc
    10201 gcatgggaac cggccgccgg gtagacgacc gggtggctgc cttccttttc gaccttggcg
    10261 tcatcccagt tcgcccgctc tcccccggcg tgctggaaga cgatcatctc gtgaggctcc
    10321 tgcttcagcg ccccggccgg tgtgttcgca tcaaaggtga tctgcatgcc ctcccagtcg
    10381 ctttcatgca gatcgttgaa ctggttgaag taccagtaga accagtactg gagtgccagg
    10441 ccggaatgtc ccttttcgcg ggcgatgtgg gcgtagaccg cgaccggtgc ccggccttca
    10501 cgccgcatcc ggtcgaagtc cttcgcatac acgcaggtct ctccgagcgg gtcgccgggc
    10561 aggtcgatgt aggcctcctc gccccggttc ctgatgtctc tcagggtcgg tgccctggtg
    10621 atcaggctgg aatcctgctt gccgttgttc cgcatcagct tcacagcggg gttgccgaac
    10681 aacgcgtcga ccgtcatcac ctggtactgc tcgaccttgg tgtcgcagag gttctcgccc
    10741 tgcttgcgga tcatcatcac cgggacgtac ttctcggcca gctcgcgggc cgcgacggac
    10801 ccggctccgg cagcgtcggc cggagcgatc ccggcagatg aaagcgtcag gagggcaagg
    10861 gccaggggca gcgaaacgat tgcggccaag atgattggag tgtcgaggca gtcgatccac
    10921 cgccatcgtc cgcggtagac gcggacc
//
LOCUS       DAOQKN010000079        10779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_2885_length_10779_cov_5.468762, whole genome shotgun sequence.
ACCESSION   DAOQKN010000079 DAOQKN010000000
VERSION     DAOQKN010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 10779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10779
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2885_length_10779_cov_5.468762"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="PLJ64_06795"
     CDS             complement(<1..685)
                     /locus_tag="PLJ64_06795"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HNC93222.1"
                     /translation="MTRKLVLLFAAFAAIGLIGAGSAAAAPPPLGISCTVAGDNSLQC
                     GSTSPRSTAETWDGTPIDVNIALPDPAEFGTGPYPLVMMFHGYGQSKLPFAQMAHYTD
                     KGYAAFTMSDRGMAESCGSVASVAADPDGCDGQYIHMLDDRYEVRDAQYFAGELVDEG
                     WVDPARIAATGGSYGGGMSMALAALKDRTMLPNGFLVPWKSPAGTPISLAVATPLVPW
                     TDLAYSLSPN"
     gene            830..1678
                     /locus_tag="PLJ64_06800"
     CDS             830..1678
                     /locus_tag="PLJ64_06800"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNC93223.1"
                     /translation="MPAVVLALLSSVSYGFSDYLGGLKSRNIPVLTVLLVSQAAALVA
                     ITATLALAVGDIPGNRYLAYGVLAGIAEVVGLSALYHGLSVGKMSIVAAVAATAPVVP
                     VIASVISGDAPGALQWFGIVIAIGGVSLLALGSGGENEPHARKAPSVSIIFGLLTAAG
                     LGCFLLAMDHSAEGSAQWALITARATSVGIFTLAFLAIRPEGELKLQQWGWLILIGLL
                     VMVADSFYAVATVKGVLSVVAVLSSLYPVVTILLARFHLREHLTRHQIAGIAVVLVGA
                     AALSVG"
     gene            complement(1718..2575)
                     /locus_tag="PLJ64_06805"
     CDS             complement(1718..2575)
                     /locus_tag="PLJ64_06805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC93224.1"
                     /translation="MGIPGALRNVGGSSKDLAARQTRPPAAIALPAVFAVLFLILPLA
                     AVVIRLPWGSVGEILTRSDTVEALALSLGTASVATVLCVIFGVPLAWWLDWLGRSPRS
                     LVYRLTRTLVLVPLVLPPVVGGVALLMLLGRKGVIGGPINDLTGITLPFTTPAVVIAQ
                     AFVALPFLVLTVEGALRSIDRSLDEVALDLGATPTRSFLLVILPIVRPAVFSGAVLCF
                     ARALGEFGATITFAGNLPGVTRTMPIATYTTMQTDPDAALTLSLILLAVAVVILVAMR
                     DRWTRGAFG"
     gene            complement(2554..3324)
                     /gene="modA"
                     /locus_tag="PLJ64_06810"
     CDS             complement(2554..3324)
                     /gene="modA"
                     /locus_tag="PLJ64_06810"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNC93225.1"
                     /translation="MAGRSLLAILATLAVAVALPACGSSDDSGSGDKEDLTVFAASSL
                     TEAFTNLGKQYEAAHEGAVVKFNFGSSSDLAAQIDQGAPADVFASADEPNMQKVVDSD
                     MADGEPQDFASNVLEIAVPRDNPGKVTGLDDFAEKDLKLAVCDPEAPCGNAATEVFGN
                     AGVDASIDSYEPDVKSVLTKVELGEVDAGLVYHSDVLAAKDRIDSIAIPEQDQFINVY
                     PIVVVKGAGNAQGGQDFIDLVLGDDGQNELAKWGFRAP"
     gene            complement(3326..3721)
                     /locus_tag="PLJ64_06815"
     CDS             complement(3326..3721)
                     /locus_tag="PLJ64_06815"
                     /inference="COORDINATES: protein motif:HMM:NF015424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HNC93226.1"
                     /translation="MSANEFKVAEAAALLGVSDDTIRRWAADGRLTLREGKGGVKVVD
                     GRELARLASESTLEAEGGATFSVRNRMHGIVTAVKTDAVMAQVDIQAGPFRLVSLISS
                     EAVEQLGIKVGKAVFATAKATNVGVEIAS"
     gene            complement(3802..6123)
                     /locus_tag="PLJ64_06820"
     CDS             complement(3802..6123)
                     /locus_tag="PLJ64_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HNC93227.1"
                     /translation="MSERPPEQDFNDDGIKVGPPKKVAAGLPSVAFAMGPMLRNAGPR
                     KTLRSSFTMNHKRGFDCPSCAWISEDRPPAIDFCENGMKSLNSEIAPVTVPREFWAGN
                     SLAGMRDKSEFWLGRQGRITEPVYRPKGSGHYEVIGWEKAFDLIADRLRSLDSPDQAV
                     FYTSGRIMNEPAFLYQLFAREYGTNNLPDCSNMCHEATGAGMGWSIGVGKSTVSYSDF
                     EKADLIMVMGQNPGTNHPRMLNALEGAKRKGARVVGINPLPEAALLRYRNPQKPRGLI
                     GKGTVISDQFVQIRSGGDQHLLRAIGKRVLMAEEAAPGTVIDHEFIEQHCEGFEAYRE
                     AALALDDGEVLAATGLSAGEIDELADRYIRSKATIITWALGLTQQKSAVETITEIMNL
                     LFLKGNIGRPGAGASPIRGHSNVQGDRTMGVWEQMGDWFLDAIRDEFGFEAPREPGHD
                     TVRTADAMERGEVAFFMSMAGNFVAAVSDSTRAEAGMERVGMTVHVSTKLNRSHVTCG
                     EESLILPVLGRAERDEQASGSQVVTAEDSVCRITASKGNLDPVAEGLISDVSVVSRLA
                     RKVIPDSKVDWAAFEEDYDRIRDSISRVVPGFEDFNRKLEQEGTFILPHGPRDSRTFP
                     TPSGKARFATAEVPVLEVPAGRLLLNTVRAHDQHNTAILGLNDRYRGIRKGRFVIFVS
                     PEDLAELEIEDGQTVDVFSERTGEEDRVLRGYRAVAYPTKRGCAAMYFPEANELVHRS
                     AVDRRSNCPAYKDVIIRIEPGTNRVAGGRPVAN"
     gene            complement(6120..7010)
                     /gene="fdhD"
                     /locus_tag="PLJ64_06825"
     CDS             complement(6120..7010)
                     /gene="fdhD"
                     /locus_tag="PLJ64_06825"
                     /inference="COORDINATES: protein motif:HMM:NF001943.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HNC93228.1"
                     /translation="MGRVVSERMVTRFRSGSRPSRRRDRLAGEEPLEIRVNGTPLAVT
                     MRTPGSDYELAAGFLVSEGVISKAADFGGATYCRGGSGPGAANEYNVLDVLLGPGVEP
                     PSPELTRNFYTTSSCGICGKSGIGEIGVDSAFDITGFEGEVELELLLSLPDRLRGSQE
                     VFDETGGLHAAAIFDAASGEMLVSREDVGRHNAVDKVIGWALMEGKLPLTGCVLQVSG
                     RAGFELVQKAKMAGLPVFSAVSAPSTLAVELASASGITLAGFVRGDSLVAYSRPDRIR
                     SVGETPGTAGPDQLPRAVAP"
     gene            7057..8304
                     /locus_tag="PLJ64_06830"
     CDS             7057..8304
                     /locus_tag="PLJ64_06830"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNC93229.1"
                     /translation="MITVAEHLNRILAMVPVLEVEKAGLAEARGRFLASPVTAAIPVP
                     PWTNSAMDGYAVRFDDLAGASQQSPVTLRVVGDLPAGTGEDPAIGPGEAVRIMTGAPV
                     PAAADTVIPQEMTDGGLEEVRIEEQLPRGRHIRREGEDRAQGDQVSPAGIELSPESLA
                     AMASAGIGEVIVARRPRVAVISTGDELVAPGEPVLRGRIPDSNGLLVTMLAADAGALA
                     EADHAGDGPGQLEAALGRNGGCDALVLTGGVSVGAFDPVKALFEGDESVRFDRVAMQP
                     GKPQAFGRLGESGPLVFGLPGNPVSAWVSFQMFVRPALRKMQGAARLFPEAVPARATE
                     DWKTPQGRMQVIPVRISDENERKVLPAATGGSGSHLIASLAAADGYALVEPEVEKIKA
                     GDLVPTVRLRDPEQFNHPQRRQP"
     gene            8301..8771
                     /gene="moaC"
                     /locus_tag="PLJ64_06835"
     CDS             8301..8771
                     /gene="moaC"
                     /locus_tag="PLJ64_06835"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HNC93230.1"
                     /translation="MTFTHLDESGQARMVDVTAKQPTVRSATASGFVRCPPAVIAALR
                     DEDLPKGDALAVARVAGIQGAKKCAELLPLAHVIGVHGAAVSFETGDEGVSVSATVRT
                     ADRTGVEMEALTAVSVAALTLIDMFKGIDKGVTIESVRLEEKTGGKSGTWRRKA"
     gene            8771..9352
                     /locus_tag="PLJ64_06840"
     CDS             8771..9352
                     /locus_tag="PLJ64_06840"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HNC93231.1"
                     /translation="MPEPATAVVLAGGRGSRLGGAEKGEIRLDGRRLIDIVVAAARES
                     GCERIVIAGDIESEGAITIREDPPFGGPAAGLAAALPAIEDDWILLLACDLPHARLLI
                     DLLTGAFREVGPEVDGLIALRNNHPQWLAGFYRRRSIEDALGRAGDPNDISMKSLFGG
                     LKLREVQDPEGLSRDLDTPEDLEEFAKKGEVEK"
     gene            9349..9627
                     /locus_tag="PLJ64_06845"
     CDS             9349..9627
                     /locus_tag="PLJ64_06845"
                     /inference="COORDINATES: protein motif:HMM:NF040429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6457 domain-containing protein"
                     /protein_id="HNC93232.1"
                     /translation="MNQENPKHLPPEALDNWLEKVSGLLGIEEEIDVGAVLDLTRDVA
                     HDVARPAAPLTTFALGLALGRLPADQFEPKFAELQAALTRLAAEQQEA"
     gene            9633..9869
                     /locus_tag="PLJ64_06850"
     CDS             9633..9869
                     /locus_tag="PLJ64_06850"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNC93233.1"
                     /translation="MSVRFFAGAAEAFGATETQVEGADTVAGLIESLAGADERLRMVL
                     DQCSFFVDGEHSTALEKPLPEDGRIDVLPPFAGG"
     gene            complement(9888..>10779)
                     /gene="moaA"
                     /locus_tag="PLJ64_06855"
     CDS             complement(9888..>10779)
                     /gene="moaA"
                     /locus_tag="PLJ64_06855"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HNC93234.1"
                     /translation="VPWIPKQSVLTPDEIERIVRVAAAEGIETVRLTGGEPLISPNLV
                     EIVRRIAAIEGPNGPIEVAMTTNGIRLPDFIPGLEEAGLARLNISIDTLDPDRFAELT
                     RRDRLIDVLAGIEAARRSKLRPLKLNAVVMRGVNEDEVVDLVEFAIEHDAQMRFIEHM
                     PLDIGHTWDREEMVPREEILAALGKRFRLEPLGGRGGAPAEVFGIDGGPFTVGVIASV
                     SAPFCAACDRLRLTADGQLRNCLFAREELDLKGALRSGASDEGIRDILRAGVAAKRAG
                     HGIDSPDFTQPDRGMNAIGG"
BASE COUNT         1785 a         3598 c         3570 g         1826 t
ORIGIN      
        1 cgttgggcga gagtgaatag gcgaggtcgg tccaggggac cagcggagtc gccaccgcca
       61 ggctgatcgg cgtgccggct ggactcttcc agggcacgag gaaaccgttt ggcagcatcg
      121 tgcggtcctt cagcgcggcc agcgccatcg acatgccacc gccgtacgaa ccgccggttg
      181 cggcgatcct ggctggatcg acccagccct cgtcgaccag ctcccccgca aagtactggg
      241 cgtcccgcac ttcataacgg tcgtccagca tgtggatgta ctggccgtcg cagccatccg
      301 gatcggctgc gaccgaggcg acgctgccgc aggactcggc catgccgcgg tcgctcatcg
      361 tgaaagccgc gtaacccttg tccgtgtagt gggccatctg ggcgaaagga agcttgctct
      421 ggccgtagcc gtggaacatc atcaccagcg ggtagggccc ggtgccgaat tcggcggggt
      481 cgggcagggc aatgttcacg tcaatcggcg ttccgtccca ggtctccgcc gtgctgcgcg
      541 ggctggtgct gccgcattgg agcgagttgt ccccggcaac cgtgcacgag atgccgagcg
      601 gcggcggtgc cgcggcagcc gaaccggctc caatcaggcc gatcgctgcg aacgcggcga
      661 aaagcagaac aagcttcctg gtcatcgggt ctcctcccgt caatggagga gcccctcgct
      721 tcctccacta cccaaactta tagcaggcag tcctgcattt atctaccagg gtgaatgcaa
      781 gaacagacgc gacggcatcc cgggggcacc accacatagg ctgcccggaa tgccagcggt
      841 cgtgctcgcc cttctctcca gcgtttccta cggtttttcc gactatctcg gcggactcaa
      901 gagccgcaac atcccggtgc tcacggtgct gctggtctca caggctgccg ccctggtcgc
      961 gatcacggcg accctcgcgc tggcggtcgg tgacatcccc ggcaaccggt acctcgccta
     1021 cggggtgctg gccgggatcg cggaggtggt tggtctgtct gccctctacc acgggctttc
     1081 ggtcgggaag atgagcatcg tcgccgcggt cgctgcgacc gcgccggtgg tcccggtcat
     1141 cgcttcggtg atcagcggcg acgctccggg tgcgctgcag tggttcggaa tcgtgatcgc
     1201 gatcggcggc gtaagcctgc tcgcgctcgg cagtggtggt gaaaacgagc cccatgcccg
     1261 caaggcaccg tcggtcagca tcatcttcgg cctgctcacc gcggccgggc tgggctgctt
     1321 ccttttggcg atggatcact cggccgaggg cagtgcccaa tgggcactta tcaccgcccg
     1381 cgcaacctcg gtcggcatct tcaccctcgc cttcctggcg atccggccgg aaggtgaact
     1441 gaagcttcag caatggggct ggctgatttt gatcggactg ctggtcatgg ttgccgactc
     1501 cttttacgcc gtggccacgg tcaagggcgt gctcagcgtg gtcgcggtcc tgagctcgct
     1561 ttatccggtg gtcaccatcc tgctggcccg ctttcacctg cgcgagcatc tcacccgcca
     1621 ccagatcgcc ggcatcgccg tggtgctggt cggcgcggct gccctctcgg tcggctagcc
     1681 ccgaccaggc ggcttcaacc cgcggcggac cggtcgatca gccgaaggcg cctctggtcc
     1741 agcggtcgcg catcgcgacg aggatcacca ccgcgacggc gagcagaatc aacgaaaggg
     1801 tgagggccgc gtcggggtcg gtctgcatgg tggtgtaggt cgcgatcggc atggtccggg
     1861 tgacaccggg gagatttccg gcgaaggtga tcgtcgcgcc gaactcgccg agggccctgg
     1921 cgaagcagag cacggccccg gaaaagacgg ccggtcgaac gatcggcagg atgaccagca
     1981 agaaggaccg ggtcggtgtc gccccgaggt ccagcgccac ttcatcgagg ctgcgatcga
     2041 tcgaccgcag cgccccttcc accgtgagca ccaggaacgg cagggccacg aatgcctggg
     2101 cgatcacgac cgcgggagtc gtgaaaggca gggtgatgcc ggtcaggtcg ttgatcgggc
     2161 cgccgatcac cccctttcgc ccgagcagca tcagcaaggc caccccgccg accaccgggg
     2221 gcagcaccag cgggaccagg acgagagtcc tggtaaggcg ataaaccagg ctgcggggtg
     2281 agcggccgag ccagtcgagc caccaggcca ggggtactcc gaagatcacg cagagaacgg
     2341 tcgcgaccga cgccgtgccg agcgaaaggg ccagcgcctc gacggtgtcg cttcgggtga
     2401 ggatctcccc gaccgaaccc cagggcaacc tgatgaccac cgccgccagg ggcaggatca
     2461 aaaagagaac cgcgaaaacc gccggcagcg cgatcgcggc cggcggcctg gtctggcggg
     2521 ccgccaggtc cttcgaggac ccgccgacgt ttctcagggc gcccggaatc cccacttcgc
     2581 cagttcgttc tgaccgtcgt cgccgagcac gaggtcgatg aagtcctggc cgccctgggc
     2641 gttgccggca cccttgacca cgacgatcgg gtagacgttt atgaactggt cctgctcggg
     2701 aatcgcgatc gagtcgatcc tgtctttggc cgcgaggaca tccgagtgat agacgagccc
     2761 ggcatcgacc tcaccgagtt ctaccttggt cagcaccgat ttcacgtcgg gctcgtaact
     2821 gtcaatcgaa gcgtcgactc cggcgttccc gaagacctcc gtggcggcgt ttccgcaggg
     2881 agcttcgggg tcgcagaccg ccagtttcag gtccttttcg gcgaagtcat cgagaccggt
     2941 caccttgccg ggattgtccc tggggaccgc gatctcgagg acgttgctgg caaagtcctg
     3001 cggctcgccg tcggccatgt ccgaatccac gaccttctgc atgttcggct cgtcggccga
     3061 ggcgaagacg tcggccggcg cgccctggtc gatctgggcg gcgaggtccg agctcgaccc
     3121 gaagttgaac ttgaccaccg cgccctcatg cgcggcttcg tactgcttgc cgaggttggt
     3181 gaaggcttcg gtcagcgagg atgccgcgaa gacggtcaga tcctccttgt caccggaacc
     3241 cgaatcatcc gaactgccgc aggccggaag cgctaccgca acggctagcg tcgcgaggat
     3301 cgccagaagg ctccttccgg ccaatttacg acgcaatttc cactcccacg ttggttgctt
     3361 ttgcagttgc gaacacggcc ttgccgacct tgatgccgag ctgctcgact gcttcgctgc
     3421 tgatcagcga aacgagccgg aagggaccgg cctgaatgtc gacctgagcc attactgcgt
     3481 cggtcttgac cgcggtcacg atcccgtgca tgcggttgcg aaccgaaaac gtcgcaccgc
     3541 cttcggcctc cagcgttgat tccgaggcca gccgggcaag ttcgcggcca tcgacgacct
     3601 tgaccccgcc cttcccctcc cggagggtca accggccgtc tgcggcccag cggcggatcg
     3661 tgtcatcgct tactccgagc agggcagccg cctcggcgac cttgaattcg ttggctgaca
     3721 tgcggccaca ctatcaacac ctgattgcgg ccaaataccc aaatataagc cgtattcgcg
     3781 gcttgtagtg aggttttgtt cctagtttgc gaccgggcga ccaccggcaa cccggttcgt
     3841 gccaggttcg atccggatga tcacgtcctt gtaagccggg cagttgctcc tccggtcgac
     3901 cgcggagcgg tggaccagtt cgttggcttc gggaaagtac atcgccgcgc aaccccgctt
     3961 cgtcgggtag gcgaccgccc ggtagccgcg caggacccga tcctcctccc cggtccgttc
     4021 gctgaagacg tcgaccgtct ggccgtcctc gatctccagc tcggccaggt cctccggact
     4081 gacgaagatc acgaagcgcc ccttgcggat tccgcggtaa cggtcgttga gaccgaggat
     4141 cgcggtgttg tgctggtcat gggcccggac cgtgttcagc agcaggcggc cggcgggcac
     4201 ctccagtacc ggcacttcag cggtcgcgaa gcgcgccttc ccggaggggg tcgggaaggt
     4261 ccggctgtca cgagggccat ggggcaggat gaaggtccct tcctgttcaa gcttccggtt
     4321 gaaatcctcg aaccccggaa ccacccggct gatcgagtcg cggatccggt catagtcctc
     4381 ttcaaaggcg gcccagtcga ccttcgaatc ggggatcacc ttgcgggcca gccgggaaac
     4441 caccgacaca tccgaaatca gcccttcggc gaccggatcg aggttgccct tgctggcggt
     4501 gatccggcag accgaatcct cagccgtaac gacctggctg ccgctcgcct gttcgtcccg
     4561 ttccgccctg ccgagcaccg ggagaatcag cgactcctcg ccgcaggtga catgggaacg
     4621 gttcagcttg gttgagacgt ggaccgtcat tccgacccgc tccatccctg cttcggcccg
     4681 ggttgagtcg gacaccgcgg ccacgaaatt tccggccatg ctcatgaaga aggcgacttc
     4741 ccccctttcc atggcgtcag cggtgcgcac ggtgtcgtgt ccgggctccc gcggcgcttc
     4801 gaagccaaac tcgtcccgga tcgcgtcgag gaaccagtca cccatctgtt cccagacccc
     4861 catcgtgcgg tcaccctgaa cgttgctatg cccgcggatc ggcgatgccc cggcgccggg
     4921 ccggccgatg ttgcccttga ggaagagcag gttcatgatc tcggtgatcg tctcgaccgc
     4981 ggacttctgc tgggtgagcc ccagggccca ggtgatgatc gtcgccttcg agcggatgta
     5041 gcggtcggcc agctcgtcaa tttccccggc actcagaccg gtcgcggcga gcacctcccc
     5101 gtcatcgagg gcgagcgccg cctcgcggta ggcctcaaag ccttcacagt gctgctcgat
     5161 gaattcgtga tcgatcacgg tgccgggggc ggcctcttcg gccatcagta cccgcttgcc
     5221 gatcgcccgg agcagatgct ggtccccgcc gctccggatc tgcacgaact gatcggagat
     5281 cacggtgccc ttgccgatca gcccgcgggg tttttgcgga ttccggtacc ggagcaaggc
     5341 ggcctccggc agcggattga tcccgacgac ccgggcgccc ttccgcttgg cgccctcgag
     5401 ggcgttgagc atccgcggat ggttggtgcc cgggttctgg cccatgacca tgatcaggtc
     5461 ggccttttcg aagtccgaat agctgaccgt cgacttgccg acgccgatcg accaacccat
     5521 cccggccccg gtcgcttcgt ggcacatgtt cgaacagtcg ggcaggttgt tggtcccgta
     5581 ttcgcgggca aatagctggt agaggaacgc cggttcgttc atgatccggc cgctggtgta
     5641 gaagaccgcc tgatcgggcg aatccagcga acgaaggcgg tcggcgatca gatcaaaggc
     5701 cttctcccag ccgatcacct cgtaatggcc ggagcccttc ggacggtaga cgggttcggt
     5761 gatccggccc tggcggccaa gccagaactc ggacttgtcg cgcatcccgg ccagcgagtt
     5821 cccggcccag aattcccggg gcacggtcac cggggcgatc tcggaattga gggacttcat
     5881 gccgttttcg cagaagtcga tcgccggtgg cctgtcctcg ctgatccagg cgcagctggg
     5941 gcaatcgaag ccgcgcttgt ggttcatggt gaaggaggaa cgaagggtct tgcgcggccc
     6001 ggcgttcctc agcatcgggc ccatcgcgaa agcgacggag ggcaacccgg cggcgacctt
     6061 cttgggcggg ccgaccttga tcccgtcgtc gttgaagtcc tgctccggcg gccgttcgct
     6121 caaggcgcga ccgcccgggg aagctggtcc ggcccggcgg tgcccggggt ttctccaacc
     6181 gagcggattc ggtcgggccg gctgtaggcg accagtgaat cgccccggac gaagccggcc
     6241 agggttatcc ccgaagccga ggccagctcc accgcgaggg ttgacggtgc ggaaacggcc
     6301 gagaagaccg gcaaaccggc catcttcgcc ttttggacca gttcgaagcc ggcccggccg
     6361 gagacctgga gcacgcagcc ggtcagtggc aacttccctt ccatcagcgc ccagccgatc
     6421 accttgtcga ccgcgttgtg acggccaaca tcctcccggg aaaccagcat ttcgccgctg
     6481 gctgcgtcaa agatggcagc agcgtgaagg ccgcccgtct catcgaagac ctcctggctt
     6541 ccccgcagcc ggtccggcag gctcagcagg agttcaagct cgacttcgcc ctcgaacccg
     6601 gtgatatcga aggccgaatc gactccgatc tcgccaatcc cggacttgcc gcagatgccg
     6661 caggaactcg tcgtgtagaa gttcctggtc agctcgggag aaggtggctc caccccggga
     6721 ccgagcagca cgtcgagcac gttgtattcg ttcgccgccc ccggacccga accgccacgg
     6781 caataggtgg cgccaccgaa atcggccgcc ttcgagatca ccccttccga aacgagaaag
     6841 ccggccgcca gttcgtagtc gctgccgggc gtcctcatgg tcaccgccag cggcgtgccg
     6901 ttgacccgga tctcaagcgg ctcctcgccg gccagtctgt cccggcggcg acttggcctt
     6961 gaaccggagc gaaaccgggt gaccatgcgc tctgaaacga ctcggcccat tgggtgatca
     7021 tagatgccac cccgctccgt caatagcatc tgccccgtga tcacagtcgc cgaacatctc
     7081 aaccggatcc tcgccatggt gcccgtgctc gaagtcgaga aggcgggtct ggcggaagcg
     7141 cgcggccgtt ttctggccag ccccgtcacc gcggcgattc cggtgccgcc atggaccaat
     7201 tcggcgatgg atggatacgc cgtgcgcttc gacgatctgg ccggagcgag ccaacagtca
     7261 ccggtgactt tgcgggtggt cggcgacctc ccggccggca ccggcgagga cccggcgatc
     7321 ggtcccggtg aagcggtgcg gatcatgacc ggcgcaccgg tcccggccgc ggcagacacg
     7381 gtcatccccc aggagatgac cgacggcggc ctcgaggagg tccggatcga ggaacagctg
     7441 cccaggggcc gccacatccg ccgggagggc gaggaccggg cccagggtga tcaggtctcg
     7501 ccggccggaa tcgagctttc gccggaatct ctggccgcca tggcctcggc cggcatcggc
     7561 gaggtgatcg tcgcccgcag gccacgcgtg gcggtgatca gcacgggcga tgaacttgtc
     7621 gctcccggcg aaccggtttt gcgcggccgg atcccggact cgaatggcct gcttgtgacc
     7681 atgctggctg ccgacgccgg tgccctggcc gaggccgacc acgccggcga cgggcccggt
     7741 caactcgagg cggcgcttgg ccggaacggt ggctgcgacg cattggtcct gaccggcggg
     7801 gtcagtgtcg gagccttcga cccggtcaag gcactcttcg aaggagacga atcggtccgc
     7861 ttcgaccggg ttgcgatgca gcccggcaaa ccgcaggcct tcggccgact gggtgaaagc
     7921 ggaccgctcg tcttcgggct gcccggcaac ccggtcagcg cctgggtttc ctttcagatg
     7981 ttcgtccgtc ccgccctgcg aaagatgcag ggggcggccc ggctctttcc cgaggcggtc
     8041 ccggcccggg caacggagga ctggaagacc ccgcaaggcc ggatgcaggt gattccggtg
     8101 cggatctccg acgagaacga gcgaaaggtg ctgccggctg cgaccggtgg ctccggctcc
     8161 cacctgatcg ccagcctggc ggcggccgac ggttacgccc tggtcgagcc cgaggtcgag
     8221 aagatcaagg ccggcgatct cgtaccgacc gtccgcctgc gtgacccgga gcagttcaac
     8281 cacccccaga ggagacaacc ttgaccttca cccacctaga cgaaagcggc caggcccgga
     8341 tggtcgacgt gaccgccaag cagcctaccg tgcggtcggc cacggcttca ggtttcgtgc
     8401 gctgcccgcc agcggtgatt gcggccctgc gcgatgagga cttgccgaag ggtgatgcgc
     8461 tggccgtggc ccgggtggcc ggtatccagg gggccaagaa atgcgccgag ctcctgccgc
     8521 tggcccacgt gatcggcgtg cacggtgccg cggtgtcatt cgagaccggg gacgaagggg
     8581 tgagcgtcag cgcgacggtg cgcaccgccg accggaccgg agtcgagatg gaggccctga
     8641 ccgcggtctc ggttgccgcc ctcaccctga tcgacatgtt caaggggatc gacaagggcg
     8701 tgacgatcga atcggttcgc ctcgaagaga agaccggcgg caaatccggc acctggcggc
     8761 ggaaggcctg attcccgaac ccgccactgc ggtcgtcctc gccggcggcc ggggctcccg
     8821 gctcggcggc gccgagaagg gcgaaatcag actggacggc cggcgcctga tcgacatcgt
     8881 cgtggctgcc gcccgcgaaa gcggctgcga aaggatcgtc atcgccggcg acatcgaatc
     8941 cgaaggggcg atcacgatcc gtgaggatcc gcccttcggc gggccggcgg ccgggctcgc
     9001 ggctgccttg ccggcaatcg aggacgactg gatcctcctg ctggcctgcg acctccctca
     9061 tgcccggctt ctgatcgacc tgctgaccgg cgccttccgc gaggtcggcc cggaagtcga
     9121 cgggctcatc gcgctgcgga acaaccatcc tcagtggctg gccggctttt atcgacgccg
     9181 ctcgatcgaa gacgcgctgg gccgggcggg tgacccgaac gatatttcga tgaaatccct
     9241 tttcggaggt ctgaagctac gagaggtcca ggatccggaa ggattgtccc gggaccttga
     9301 caccccggaa gatcttgaag agttcgcaaa gaaaggcgag gtggagaaat gaaccaggag
     9361 aatccgaagc atctgccgcc ggaggccctc gacaactggt tggagaaggt ttccgggctg
     9421 ctcgggatcg aggaggaaat tgacgtcggg gccgtgctcg acctgaccag ggatgtagcc
     9481 catgacgtgg cccggcccgc cgcgccgctc accaccttcg cgctcggcct ggcgctgggc
     9541 cggctgccgg ccgaccagtt cgagccgaaa ttcgccgagc tgcaggctgc cctgacccgg
     9601 ctcgcggcgg aacagcagga agcttgaccc gggtgtcggt caggttcttt gccggggcgg
     9661 ccgaagcttt cggtgctacc gaaacgcagg tcgaaggggc cgacaccgtg gccgggttga
     9721 tcgagtcact ggccggggcc gacgaacggt tgcggatggt cctcgaccag tgctcgttct
     9781 tcgtcgacgg tgaacacagc accgcgctcg agaagccgct tccggaagac ggccgaatcg
     9841 acgtgctgcc gcccttcgcc ggcggctgag cgggcaaccg ggcggcttca gccgccgatc
     9901 gcgttcatgc ctcgatccgg ctgggtgaag tccggactgt cgatgccgtg gccggcccgc
     9961 ttggcggcaa ccccggcccg caggatgtcg cggatcccct cgtcggaagc accgctgcgc
    10021 agggctcctt tcaggtcgag ctcctcgcgg gcgaagaggc agttgcgcag ctgaccgtcg
    10081 gcggtcagcc gcagccggtc acaggccgcg cagaaggggg cggagaccga agcgatcacg
    10141 ccgaccgtga aggggccacc gtcgataccg aagacctcgg ccggcgcccc gccgcggccg
    10201 cccagtggct caagccggaa acgcttcccc agcgcggcca ggatctcctc gcgcggaacc
    10261 atctcttcac ggtcccaggt gtggccgatg tccagcggca tgtgctcgat gaagcgcatc
    10321 tgcgcgtcgt gctcgatcgc gaattcgacc aggtcaacga cctcgtcctc gttcactccg
    10381 cgcatcacga cggcgttcag tttcagcggc cggagtttcg agcggcgggc ggcttcgatc
    10441 ccggcgagca cgtcgatcag ccggtcgcgc cgggtcaact cggcgaagcg atccgggtcg
    10501 agcgtgtcga tgctgatgtt gagccgggcc aggcccgcct cctcgagacc ggggatgaag
    10561 tcgggcaggc ggatgccgtt ggtggtcatc gccacttcga tcgggccgtt cgggccctcg
    10621 atggccgcga tccgccggac tatctcgacc aggttcggac tgatcagggg ctcgccgccg
    10681 gtcagcctga ccgtttcgat tccctccgcg gcggcaaccc ggacgatccg ctcgatttcg
    10741 tccggggtga gaaccgactg cttggggatc cagggcacg
//
LOCUS       DAOQKN010000080        10226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_3094_length_10226_cov_4.450890, whole genome shotgun sequence.
ACCESSION   DAOQKN010000080 DAOQKN010000000
VERSION     DAOQKN010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 10226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10226
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3094_length_10226_cov_4.450890"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1632)
                     /locus_tag="PLJ64_06860"
     CDS             complement(<1..1632)
                     /locus_tag="PLJ64_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93235.1"
                     /translation="MPEPREKSANKASAGLPATLVLTLSVTVGIFLLLLAPVYLLLPT
                     TPMPEPLPPQHQNAETLLFGLLILAGVATVLLLPRRLDQWAAAHSPAAADLLAACAAG
                     GLGLLVVLARLAHHLAGRNLLVVLGLSIIWWLVMVAIFAKGGEGLRALAAGPGRGRIL
                     LLAATAAVAVGLLAPVTFAHLNGVPLLVCLGLAGLGTWLWGRVRLPVLRGWKGVACDL
                     LLIALVLLAVPDIPIYRVGDLASQPVDSYLAEVMVFHGNLYLGAANAILHGSHLLVDE
                     VSQYGVGSIYLLAALFELIPIDYGTLSLIDAVLSAVVFAAGYLILRMAGTGRLLGLGA
                     MAVTVIVLAWGLTFPIGGIVQNGAIRFGLLPVSLVFFRIASIRLAGGGRAGLARVSGW
                     LAWAVVGLSAIWALEALLYTTATLTGLVLAEAMTRPPGSRRRWILGRVVRALIAWASF
                     HLAFAIYILIAAGQLPDWGMYISYLRDFLVGKVGDLTYDFEPWSPGLLVGFGYLTSAT
                     ATALAIWRRRDWFDANQVAFIALAGLSLYGLIQFSY"
     gene            1687..2964
                     /locus_tag="PLJ64_06865"
     CDS             1687..2964
                     /locus_tag="PLJ64_06865"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNC93236.1"
                     /translation="MRDSTPRPGDRRLEESEREAGQVLPPPPGHPRFPLLDPMRAIAA
                     ISVMLVHVAIFSGGFGPWYKWVLAHLEIGVPFFFLLSGFLLYRPMLAARVSGLPAQRI
                     RDYARNRFFRIFPLFWFILIVTAIVPGMYGAFTGNWWVYFGLLQNLPVYSPDGECVTN
                     PFRCGIPPSWTLGIEVMFYFLLPFFAAGMTMIQNRFRSTPSDGFFSRWVKIEFAALLL
                     FSAISFVIQSSSFNGGVDQWLFFSPIGRAWWFALGMVLAVISVRTAETGRDVALSRWG
                     REHGGWCWLAAIGLYLFGTYVLFSHGPTLAAPVGDLNEYLLQYFFFGVISFLALMPAV
                     FGQIGSGVATRVLAHPVPVRLGLISYGIFLWHYPVLVALNDLDATSWATSAQFPVMAI
                     ATFVLTVALSLITYYLLERPLMRWSRSRSKKRA"
     assembly_gap    2967..3066
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3158..3940)
                     /locus_tag="PLJ64_06870"
     CDS             complement(3158..3940)
                     /locus_tag="PLJ64_06870"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase E"
                     /protein_id="HNC93237.1"
                     /translation="MKGRILVMGGHEFDRLDGNEAIVEHVISLTGMKAPKICLLPTAS
                     GDPEDQISRFRRSFGSRGCEVSDISLFRLGANPIDVSAHLMKQDAIYVGGGSLVNLVA
                     VWRPHGIAGLIEECLERGVMVVGQSAGAMCWFEAGITSSSGRPEPAEGLGVLKGSLCV
                     HYHRDPERRTRYLAEVGKSMSAGYGADDQTGLLFTDGELTEAFTAREGAGVWKVTPAA
                     KGGVEEPVEAVDIRPKSKPDTPGDPVEDFRNLSKWRQSATIR"
     gene            complement(3975..4745)
                     /locus_tag="PLJ64_06875"
     CDS             complement(3975..4745)
                     /locus_tag="PLJ64_06875"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase E"
                     /protein_id="HNC93238.1"
                     /translation="MGTRRQIVAFGGGGFSMESGNPLLDDFVVQATGLKKPRVCFMPT
                     ASGDADHYLVRFYNAFRGRGEASHISLFRRERGAESIRDHLLSQDLIYVGGGSVISML
                     GVWRAHGIDRILREAWERGIVLCGLSAGSLCWFEECVTTYQKETRVTIGLGLLPWSNQ
                     VHFELGSTAHKAFEEKLKEGLRPAYAATDGAAFHFIDTELAQVVASRPEARAYRIELK
                     GDHPKTTQLATRYLGSPDQVPLVEESPIPWASTPIEAA"
     gene            5026..6150
                     /locus_tag="PLJ64_06880"
     CDS             5026..6150
                     /locus_tag="PLJ64_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93239.1"
                     /translation="MPTHSPTLASQKSRQLGLLLLALAAILASFAATAPGSKASPVSL
                     TFDNGRISMGFFQDRTVLPADSSFPSPDLPTPQRTDIQLNGDLTDGNISIPAATNTGI
                     QFPYMNVKHPLQPDLKIPFTFRLNQDGLTGTFNQETGAMTLEGKLDIIVITGTGTNFP
                     LPDSLDDVGVPPLGLFARCRYDNVPVKLSTAKSDPFNGQPFADGFGTNGALAASWKSL
                     TPSVSENGGTCGDLNQLTTSIGGLWLSNAVAEPKPQPEGPKPSCKTDLSLCPEQKYTL
                     IDGVQLKPGRKTVKPGQTVTLTVKVHNSGNKTARNLKVKVKSTNKSFKVPKKVTLTVP
                     AGRSAKRKFKVRIKGNAKGWGRITAVNNGWPGHTYLKVRP"
     gene            6209..7468
                     /locus_tag="PLJ64_06885"
     CDS             6209..7468
                     /locus_tag="PLJ64_06885"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNC93240.1"
                     /translation="MRESVTRPEHHLSDPGKEARARSGIPIVPAFDGYRAWAIMAIVM
                     LHMTAISGVAGPGVENAFTRLIWGALGNAVELLFIVSGFVVFLPTVARRGDFGSIASF
                     AIRRGARLLPAYWMILLISLPVIAVFDPPYSALPGLRDLLLNFSALTVPVGLFVPGIS
                     IGFGLDPPLWTLSVEVGFYLVLPFVASIFFRKPVAGLLVSLAVTAAWTIAFDHVSEVT
                     DLIATAPDFGELVRLKLSSELQLPAWAYSFGLGMFGAWVWVRISDRQGRGEAETPVPG
                     SGPAPAALIALVSGLALIPLVWVIGGDYSNTRASLLLSLLFSTLVAIFMVSLSLAPRS
                     WQTPFAAQPVRNLGDISYGIYLSHMVITYTIAGELTLPQDGNLKSLLIWTAVVVPLSV
                     IYGYLSARFLEQPIRRWARKYGRRVPD"
     gene            7571..9139
                     /locus_tag="PLJ64_06890"
     CDS             7571..9139
                     /locus_tag="PLJ64_06890"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNC93241.1"
                     /translation="MRKKNPETHEEKLEYLRELRDAAINSASSEAVDKHHAKGKLTAR
                     ERIEKLLDPGSFEELDTFVRHRTYDFEMNKRRPWGDAVVTGHGTIDGRKVFVFSQDFT
                     VFGGSLGEVMAEKMCKVMDLAAKVGAPVIGLNDSGGARIQEGVVSLGAYGDVFARNVS
                     SSGVIPQISVIMGPCAGGAVYSPAMTDFIFMVKETSHMFITGPEVIKTVTGEEVEFEE
                     LGGAMTHNSKSGVAHFAADDEESCLEDVRYLMSFLPLNNMEQPPHSIPGDDPDREDPE
                     LDTFVPDDPNKPYDMRKVVAKIVDDEEFFEVHEHFAKNIICGFSRLDGHPVGIVGNQP
                     AFKAGVLDIESSEKAARFVRTCDAFNIPIITFTDVPGFMPGTDQEWNGIIRHGAKLLY
                     AYTEATVPKLTVVTRKAYGGAYDVMASKHMLADFNFAWPTAEIAVMGAEGAVNIIYRH
                     DIKNSPTPDERRATLIADYKAHFANPYAAAERGYIDDVIEPSQTRPKLIKALRTLETK
                     RVAQPRRKHGNIPL"
     gene            complement(9136..9846)
                     /locus_tag="PLJ64_06895"
     CDS             complement(9136..9846)
                     /locus_tag="PLJ64_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93242.1"
                     /translation="MFTREADQSRYQYPGAVFLLMILAGAFDGYRPGRNWLTGLASLA
                     AVSVACSLGTLSEGRKILIDISVTPKASLTAMDIAGEEAIPGYQVRLTPYLPIFMSQQ
                     RFREVEERYGRAGWSEEQIADQSDEVRRLVDAELVAALGIVPRPGRAGGSSGCRTVKL
                     NPAGSEIASVPAGTLSIAPARSDAALALGRFADQATFVIGTAKAGRSTSVDLPAGRSS
                     QPWRVRASGTGKALVCVS"
     gene            9839..>10226
                     /locus_tag="PLJ64_06900"
     CDS             9839..>10226
                     /locus_tag="PLJ64_06900"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNC93243.1"
                     /translation="MNIPGAWETSQGLGVKIGVIDAGISSTQNLLGSNFNNGDSNVGR
                     TITTGYTWGTSAFSTCTHGTSMSGLAVGPRNNLNASTGVAYKSSLHFIRGCEDVVLDK
                     SSERTGVKNALVQMGDNSTIKIISMSI"
BASE COUNT         1785 a         3547 c         3129 g         1665 t
ORIGIN      
        1 gtagctgaac tggatcaggc cgtagaggct gaggccggcc agcgcgatga aggcgacctg
       61 gttggcgtca aaccagtcgc ggcggcgcca gatcgcaagc gccgtcgcgg tcgccgaggt
      121 caggtagccg aagccgacca gcaaccccgg tgaccagggt tcgaagtcat aggtcaaatc
      181 accgaccttg ccgaccagga agtcccgcag atacgagatg tacatgcccc agtccggcaa
      241 ctggccggcc gcgatcagga tgtagatcgc gaaggcgagg tgaaacgagg cccaggcgat
      301 cagggcgcgg acgactctcc ccagaatcca gcggcgacgg ctccccggcg gccgggtcat
      361 cgcctcggcc agaaccagcc cggtcagggt ggcggtcgtg taaagcagcg cctccagggc
      421 ccagatcgcc gaaagcccga ccaccgccca ggcaagccag cccgagaccc gggccagacc
      481 ggctcgcccc ccgccggcca gccggatcga ggcgatccgg aagaagacca gcgacacggg
      541 aaggaggccg aaccggatcg ccccgttctg gacgatcccg ccgatcggga aggtgagacc
      601 ccaggcgagc acgatcacgg tcaccgccat cgctccgagc ccgagcaggc gaccggtccc
      661 ggccatccgc aggatcaggt aaccggccgc gaagacgacc gccgaaagca ccgcgtcgat
      721 caggctcagg gttccgtagt cgatcgggat caactcgaag agggcggcca gcaggtagat
      781 cgaaccgacc ccgtactggg aaacctcgtc gaccagcagg tggctgccgt ggaggatcgc
      841 gttggccgcc ccgaggtaga ggttgccgtg gaagaccatc acctcggcca gatatgaatc
      901 gaccggctgg gaggcgaggt ctccgacccg gtagatcggg atgtccggca ccgccaggag
      961 caccagggcg atcaacagca ggtcacaggc gactcccttc cagccccgga ggaccggcag
     1021 gcggacccgc ccccacagcc aggtgccgag cccggccagg cccaggcaga ccagcagcgg
     1081 caccccgttc aggtgggcga aggtgaccgg cgccagcaac ccgacggcca ccgcggcggt
     1141 cgcggcaagc agcaggatcc ggccgcggcc agggccggcg gccagagcgc gcaggccctc
     1201 gccccccttg gcgaatatcg ccaccatgac cagccaccag atgatcgaga ggccgagaac
     1261 caccagcagg ttcctgccgg ccaggtgatg ggccagccgg gccagcacga ccaggaggcc
     1321 gagacctccg gccgcgcagg cggccagcag gtcggccgcg gccggcgagt gggcggcagc
     1381 ccactgatca agcctgcggg gaaggaggag caccgtcgcc accccggcca ggatcagcaa
     1441 gccgaacagc agggtctcgg cattctggtg ctgcggcggc agcggctccg gcatcggggt
     1501 ggtcggcagc aggagataga ccggcgcaag cagaagcagg aagatcccga ccgtgaccga
     1561 gagggtcagg accagggtcg cgggcagccc ggctgacgcc ttgttggcgg acttttccct
     1621 cggctcgggc accgggcgat cctacctgcg tcaaccggtt ctccccggtc tatagtccct
     1681 gcccgaatgc gagattcgac cccgcggccg ggcgacagaa ggcttgagga gtcggagcgc
     1741 gaggccgggc aggttctgcc gcccccgccc ggccaccccc gtttcccgct gctcgacccg
     1801 atgcgcgcga tcgccgcgat ctcggtgatg ctggtgcatg tcgccatctt cagcggaggt
     1861 ttcggcccct ggtacaagtg ggtgctcgcc cacctggaaa tcggcgttcc cttcttcttc
     1921 ctgctctcgg gcttcctcct ctaccgcccg atgctggccg cccgggtctc cgggcttccg
     1981 gcccagcgaa tccgcgacta cgcccgcaac cgcttcttcc ggatctttcc cctcttctgg
     2041 ttcatcctga tcgtgaccgc gatcgtgccc gggatgtacg gcgccttcac cggcaactgg
     2101 tgggtctact tcgggctgct gcagaacctt cccgtgtatt cccccgacgg cgaatgcgtg
     2161 accaacccct tccgctgcgg catcccgcct tcctggacgc tcgggatcga agtcatgttc
     2221 tacttcctgc tgcccttctt cgcggccggc atgacgatga tccagaaccg cttccgcagc
     2281 actccctcgg acgggttctt ttcccgctgg gtgaagatcg agttcgcggc gctgctcctc
     2341 ttctcggcaa tctcgttcgt gatccagtcc tccagcttca acggcggggt cgaccagtgg
     2401 ctcttcttct ccccgatcgg ccgggcctgg tggttcgccc ttggcatggt cctcgccgtg
     2461 atctcggtgc ggacggcgga aaccggccgg gacgtggcgc tctcgcgctg ggggcgtgaa
     2521 cacggtggct ggtgctggct cgccgcaatc ggcctctacc tcttcggcac ctatgtcctg
     2581 ttcagtcacg ggccgaccct ggccgccccg gtcggggacc tgaacgagta tctcctccag
     2641 tacttcttct tcggcgtgat ctcgttcctc gccttgatgc cggccgtctt cggccagatc
     2701 ggcagcgggg tggcgaccag ggttctcgcc catcccgtcc cggtgcgcct cgggctgatc
     2761 tcctacggga tcttcctctg gcattacccg gtgctggtcg cactcaacga tctcgacgcg
     2821 accagctggg ccacctccgc gcagttcccg gtcatggcga tcgcgacctt cgtcctgacc
     2881 gtcgcgctct ccctgatcac ctattacctg ctcgagcgcc cgctaatgcg ctggtcccgc
     2941 agccggtcaa agaagcgcgc ctgaccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnntcga gcgcccgcta atgcgctggt cccgcagccg gtcaaagaag cgcgcctgac
     3121 cgacggacac ccggaacgtg gcgccgtctt cacgggttca tctgatggtt gccgactggc
     3181 gccacttact tagattgcgg aagtcttcga ccgggtcgcc gggggtgtcc ggcttcgact
     3241 tcggccggat gtcgaccgct tcgaccggct cttcgacccc gcccttcgcg gccggggtca
     3301 ccttccacac cccggctccc tcccgggctg tgaaggcctc ggtcagttca ccgtcagtga
     3361 agagcaggcc ggtctggtcg tcggcaccgt aaccggccga catcgacttg ccgacctcgg
     3421 ccaggtagcg ggtccgccgt tccggatccc ggtggtaatg aacgcagagg ctgcccttca
     3481 gcacaccgag accctccgcc ggctcgggcc gccccgagga cgaggtgatg ccggcctcga
     3541 accagcacat cgcgccggca ctctggccca ccaccatcac gccgcgctcg aggcattctt
     3601 cgatcagccc ggcgatccca tgtggcctcc agaccgcgac caggttgacc aggctgcccc
     3661 cgccgacgta gatcgcgtcc tgtttcatca ggtgcgcgga aacatcgatc ggattcgcac
     3721 cgagccggaa cagcgagatg tcactgacct cgcaaccgcg actgccgaag gaacggcgaa
     3781 agcgggagat ctggtcttcc gggtcccccg atgcggtcgg cagcaggcag atcttcggcg
     3841 ctttcatccc ggtcagcgag atgacgtgct cgacgatcgc ctcgttgcca tcgagacggt
     3901 cgaactcgtg accacccatg accaggatcc ggcccttcat cgcagaaccc gcttgcggtt
     3961 tcggttgcct cgcatcaggc cgcttcgatc ggggttgacg cccagggaat cggtgactcc
     4021 tcgaccagcg gcacctggtc gggtgagccg aggtacctcg tcgcgagctg ggttgtcttg
     4081 gggtgatcgc ccttgagttc gatccggtag gcccgggcct cgggccggga tgccacgacc
     4141 tgcgcgagct cggtgtcgat gaagtggaag gcggcaccgt cggtcgccgc gtatgccgga
     4201 cgcaggcctt ccttcaactt ctcctcgaac gccttgtggg cagtcgagcc cagttcgaag
     4261 tgaacctggt tcgaccaggg cagcagaccg aggccgatcg tcaccctggt ttccttctgg
     4321 taggtggtca cgcattcctc gaaccagcag agcgagccgg ctgaaagccc gcagaggacg
     4381 atgccgcgct cccaggcctc gcggagaatc cggtctatgc cgtgggcgcg ccagaccccg
     4441 agcatgctga tcacgctgcc accgccgacg tagatcaggt cctgggagag caggtggtca
     4501 cggatcgatt ccgccccgcg ctcccgtcgg aagagtgaga tgtgcgaggc ctcgccgcgg
     4561 ccccgaaagg cgttgtagaa gcggaccagg taatggtcgg cgtcgccgga agcggtcggc
     4621 atgaagcaga cccggggctt cttcaggccg gtcgcctgca ccacgaagtc atcaagcagg
     4681 ggattgccgg attccatcga gaacccgcca ccaccaaacg ccacgatctg tctccgcgtt
     4741 cccatggaga aatacttccc cctccccgcc agctcccatt tggccaccgg gccagactct
     4801 gaacacagtc gtttgtccaa ccgcggatgt agccgtccca tccattcctt gtttctctgg
     4861 gacggcgggg ttcactggga gggcgggtcc gaccccgccc gacttgcagg tactcgtggg
     4921 gtccccctgt ttgaaaaccg ctcgatttgg gcaatgaaag cgacatcagg cgcgcgcaaa
     4981 ccgggcgcaa aagggaacct cgtcactcga aggagagcac ttcagatgcc cacccactca
     5041 ccgactctcg cgagtcagaa atcaaggcag ctcggactgc tgctgctcgc cctcgccgcc
     5101 atactcgcct cgttcgcggc cacggccccc ggctccaagg cctcaccggt ctcgctgacc
     5161 ttcgacaacg ggcggatcag catgggcttc ttccaggacc gcaccgtcct cccggccgac
     5221 agctccttcc cctcgcccga ccttcccacc ccgcagcgga ccgacatcca gctgaacggc
     5281 gacctgaccg acgggaacat ctcgatcccg gcggcgacca acaccggcat ccagtttccg
     5341 tacatgaacg tgaagcaccc cctgcagccg gacctgaaga tccccttcac cttccgcctc
     5401 aaccaggacg gcctgaccgg caccttcaac caagagaccg gtgcgatgac cctcgaagga
     5461 aagctcgaca tcatcgtgat caccggcacg ggaacgaact tcccgctgcc cgactcgctg
     5521 gatgacgtcg gcgtgccacc gctcggattg ttcgcccgct gccgctacga caacgtgccg
     5581 gtcaagctct cgaccgccaa gagcgacccg ttcaacggcc agccctttgc cgacggattc
     5641 ggaaccaacg gtgccctcgc agcctcctgg aaatcgctca ccccctcggt ttcggagaac
     5701 ggcggtacct gcggtgacct gaaccagctc accacctcga tcggcggcct ctggctttcc
     5761 aacgccgtgg ccgaaccgaa gccgcagccg gaaggcccga agccgtcatg caagaccgac
     5821 ctcagcctct gcccggagca gaagtacacc ctgatcgacg gggtccagct caagcccggc
     5881 cgcaagacgg taaagcccgg acagacggtg accctgaccg tcaaggtgca taactcgggc
     5941 aacaagacgg cccggaatct gaaggtgaag gtcaagtcga ccaacaagtc cttcaaggtg
     6001 ccgaagaagg tcactctcac cgtgccggcg ggcaggtccg ccaaacggaa gttcaaggtc
     6061 cggatcaaag gcaacgcgaa gggctggggc cggatcaccg cggtcaacaa cggctggccc
     6121 ggtcacacat acctgaaggt caggccgtag ccggctgagg gcccggccgg agtcgcatcc
     6181 gggctgaacc gggcctaaac tcccccgggt gagggagtcg gtaaccaggc cggaacatca
     6241 cctttccgac ccgggcaagg aggctcgcgc ccgatccggc atcccgattg ttccggcctt
     6301 cgacgggtac cgggcctggg cgatcatggc gatcgtcatg ctgcacatga cggcgatttc
     6361 cggggtcgcc gggcccggag tcgagaatgc cttcacccgg ctcatctggg gagccctcgg
     6421 caacgcggtc gaactgctct tcatcgtcag cgggttcgtc gtcttcctgc ctaccgtcgc
     6481 ccggcgggga gacttcggca gcatcgcttc ctttgcgatc cggaggggag cccgcctgct
     6541 gcccgcctac tggatgatcc tgctgatctc gctgccggtg atcgcggtct tcgaccctcc
     6601 ctattcggcc cttcccggac tgcgggacct gctgctgaac ttctccgcgc tgaccgtccc
     6661 ggtcggtctg ttcgttcccg ggatctcgat tggattcggc ctcgacccgc cgctctggac
     6721 cttgtcggta gaggtcggct tctacctggt tctgccgttc gtcgcgagca tcttcttccg
     6781 gaagccggtc gccggactcc tggtttccct ggcggtaacc gccgcctgga cgatcgcctt
     6841 cgatcacgtc agcgaggtga cggacctgat cgccaccgcc cccgacttcg gtgaactggt
     6901 caggctcaaa ctctcatccg agctgcagct gccggcctgg gcctactcgt tcgggctggg
     6961 catgttcggg gcctgggtct gggtccggat cagcgacagg cagggaaggg gcgaagcgga
     7021 aaccccggtc ccggggtcgg gccccgctcc ggccgccctc atcgccctgg tctcggggct
     7081 tgccctgatt cccctggtct gggtgatcgg cggcgactac tcgaacacga gggcctcgct
     7141 cctgctttcg ctgctcttct ccaccctcgt cgcgatcttc atggtttcgc tttcgctcgc
     7201 gccccggagc tggcagaccc cgtttgcggc acagccggtt cgcaacctgg gtgacatcag
     7261 ctacggcatc tatctgagcc acatggtgat cacctacacg atcgccggcg aactgaccct
     7321 gccccaggac ggcaatctga agagcctcct gatctggacc gccgtggtgg tcccgctctc
     7381 ggtcatctac ggctaccttt cggcccgctt tctggagcaa ccgatccgcc gctgggcccg
     7441 caaatacggg cgtcgcgtcc ctgactagac gaagccggcc catccgttcc cggattctgc
     7501 gggacggcgg gttgagcatg gaggccgggc tggaccccgc ccgactgcag atgaataaac
     7561 tgcctcggcc atgcgtaaga agaatcccga gacccacgaa gagaagcttg agtacctgcg
     7621 cgagctgcgt gacgccgcaa tcaattccgc ttccagcgaa gccgtagaca agcaccatgc
     7681 caagggcaag ctgaccgccc gcgagcggat cgagaagctg ctcgaccccg gatcgttcga
     7741 agagctcgac accttcgtcc ggcatcggac ctacgacttc gagatgaaca agcgccggcc
     7801 ctggggagac gcggtggtca ccggccacgg cacgatcgac ggtcgcaagg tcttcgtctt
     7861 ctcccaggac ttcacggttt tcggcggctc gctcggcgag gtcatggccg agaagatgtg
     7921 caaggtgatg gacctggccg ccaaggtcgg cgccccggtg atcggcctga acgactcggg
     7981 tggcgcccgg atccaggaag gcgtcgtttc actcggcgcc tacggtgacg tcttcgcccg
     8041 caatgtttcc agctccgggg tgatccccca gatcagcgtg atcatgggcc cctgtgccgg
     8101 tggcgccgtg tactcgccgg cgatgaccga cttcatcttc atggtcaagg agacctcgca
     8161 catgttcatt accggtcccg aggtgatcaa gaccgtgacc ggcgaggaag tcgagttcga
     8221 agagctcggc ggcgcgatga cccacaactc gaagtccggt gtcgcccatt tcgcggccga
     8281 cgacgaggag agctgcctcg aggacgtgcg ctacctgatg agcttcctgc cgctcaacaa
     8341 catggagcag ccgccccact cgattccagg cgatgacccc gaccgcgagg acccggagct
     8401 cgacaccttc gtccccgacg acccgaacaa gccgtacgac atgcgcaagg tcgtggcgaa
     8461 gatcgtcgat gacgaagagt tcttcgaggt ccacgagcac ttcgccaaga acatcatctg
     8521 cggcttctcg cgcctcgacg gccacccggt cggcatcgtc ggcaaccagc ccgccttcaa
     8581 ggccggcgtg ctcgacatcg aatcctccga gaaggcggcc cgtttcgtcc gtacctgcga
     8641 cgccttcaac atcccgatca tcaccttcac cgacgtgccc ggcttcatgc ccggcaccga
     8701 ccaggagtgg aacgggatca tccgccacgg agccaagctg ctctacgcct acaccgaggc
     8761 gaccgtgccg aagctgaccg tcgtgacccg caaggcctac ggcggcgcct atgacgtcat
     8821 ggcctccaag cacatgctgg ccgacttcaa cttcgcctgg ccgaccgcgg aaatcgcggt
     8881 gatgggcgcg gaaggggcgg tcaacatcat ctatcggcac gacatcaaga actcgccgac
     8941 cccggacgag cgtcgcgcca ccctgattgc cgattacaag gcccacttcg cgaacccgta
     9001 cgcagcggcc gagcgcggct acatcgatga cgtgatcgag ccgagccaga cccggccgaa
     9061 gctgatcaag gcgctcagga cccttgaaac gaagcgcgtc gcccaaccgc gccgcaagca
     9121 cggcaacatc ccgctctagc tgacacagac cagggccttg ccggtgccgc tggcccggac
     9181 ccgccagggc tgactcgacc ggccggccgg cagatcgacc gaggttgacc ggccggcctt
     9241 tgccgtccca atcacaaagg tcgcctggtc ggcaaaccgg ccgagggcca aagcggcatc
     9301 cgaccgggcg ggggcgatgc tcaaggtccc ggcgggaacc gaggcgattt cgcttcctgc
     9361 cgggttcagc ttcacggtcc ggcagcccga actccccccg gctcggccgg gccggggcac
     9421 gattcccaat gccgccacta gctcggcatc gaccaatcgc cggacctcgt cgctctggtc
     9481 cgcgatctgt tcttcactcc agccggcgcg accgtagcgc tcctccactt cccggaaccg
     9541 ctgctggctc atgaagatcg gcaggtacgg ggtcaggcga acctgatacc cgggaatggc
     9601 ctcctcgccg gcgatgtcca tcgcggtcag cgaagccttc ggggtaaccg aaatatcgat
     9661 caggatcttc cgtccttcgg aaagggtgcc gaggctgcat gcgaccgaga ccgcggccag
     9721 cgaagccagg ccggtcagcc agttccgccc cggccggtac ccgtcgaagg ctccggccag
     9781 gatcatcagc aagaagaccg cccccgggta ctggtaccgg ctctggtcgg cctcccgcgt
     9841 gaacatcccc ggcgcctggg aaacctcgca gggcttgggc gttaaaattg gggtgataga
     9901 tgcgggcatc agttccacgc aaaacctgct gggcagcaat ttcaacaatg gcgactccaa
     9961 cgtgggccgc accatcacca cgggctacac atggggcacc agcgcattca gcacctgcac
    10021 ccacggcacg agcatgagcg gccttgccgt cggcccccgc aacaacctga acgcctcgac
    10081 gggcgtggcc tataagtcga gcctgcactt catccggggc tgcgaggacg tcgtgctgga
    10141 caagtccagc gagcgcacgg gcgtgaagaa tgccctcgtg caaatgggcg acaacagcac
    10201 catcaagatc atcagcatgt ccatcg
//
LOCUS       DAOQKN010000081         9970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_3187_length_9970_cov_5.628744, whole genome shotgun sequence.
ACCESSION   DAOQKN010000081 DAOQKN010000000
VERSION     DAOQKN010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 9970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9970
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3187_length_9970_cov_5.628744"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="PLJ64_06905"
     CDS             complement(<1..735)
                     /locus_tag="PLJ64_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNC93244.1"
                     /translation="MAIQIIDRALRMGEGRKFKAYEKRVESINRIEPEMKELEDSELL
                     EEANALRERAKNGESLDDLLPESFALTREAAFRAIGQRHYDVQLIGGMVVHDGAIAEM
                     KTGEGKTLTSTLAIVLNALGGKSVHVVTVNDYLSKRDALWMKPIYDMLGVSVGWIQEN
                     DDPAARREKYACDVTYGTNSEFGFDYLRDNMAASLEQKVQRTHDFAIVDEVDNILIDE
                     ARTPLIISGAPEEAAQTYYTFARLAKQ"
     gene            complement(879..1247)
                     /gene="raiA"
                     /locus_tag="PLJ64_06910"
     CDS             complement(879..1247)
                     /gene="raiA"
                     /locus_tag="PLJ64_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNC93245.1"
                     /translation="MRIEIRGRNVEVNDDMREQVTQRFERLGEQVSSLARLEVVFSEE
                     QNPAISDKFVAEATLHLKGVTLHAHEASPEMTHTIHELAEDMRRQVKRHREKRRKRRE
                     TRKLVNRLQGRETGGAQPGL"
     gene            complement(1327..1968)
                     /locus_tag="PLJ64_06915"
     CDS             complement(1327..1968)
                     /locus_tag="PLJ64_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93246.1"
                     /translation="MDLDRIAGMFVPSRCPLCRGAIDPDLLVCGECMRELNRSPVLRG
                     EPPEGVDRIVSCADHDGAPRDLLAAFKFRHLTTLGGLIAGYMADAAGQPGEQSLLVPV
                     PPASLRTWFRGFDPVAMLTARLAGLLDIPEPPVPVLARRGSGRQRGRGRAGRLSDPPD
                     IRPTEAAGRQVGGRTVLLVDDVMTTGATLSTAAGALLLAGAGSVSALTFTRRL"
     gene            complement(2021..3037)
                     /locus_tag="PLJ64_06920"
     CDS             complement(2021..3037)
                     /locus_tag="PLJ64_06920"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HNC93247.1"
                     /translation="MKAMVLAAGLGTRLRPVTRAVPKPMVPVLNRPVMEHIIRLLARH
                     GFTEVIANIHWYPDVIRDHFGDGSGFGIELSYSFEEELLGTAGGVRNASAFFDDDFLV
                     ISGDAMTDIDLSAMREFHRSHGGMATLATRRVDDTSEFGVVITDDEGRIQGFQEKPDP
                     DEALSDLANCGIYMFNREIFDYFPGPGESKLMAPGHPDDFADWAYDVFPAMLDAGIAF
                     YAHETDAYWNDIGTIEELVQSNFDGLFGDVDLGLPEKQPLPEVWVAGEVEGLDGAEID
                     GPVLIGDGARIGEGAQLVGPLVLGEGCSIGAGAGVREAVLLSRSSLEKGAFLAGGLLG
                     PTRD"
     gene            complement(3037..4023)
                     /locus_tag="PLJ64_06925"
     CDS             complement(3037..4023)
                     /locus_tag="PLJ64_06925"
                     /inference="COORDINATES: protein motif:HMM:NF021916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HNC93248.1"
                     /translation="MRPSMLEDVLDIPNHIRDALWRVDSAQLKARPSAGFVVCGMGGS
                     AIGAELARGVLDNRLTGPMVVSRSYGLPKWVTADWAVLASSYSGDTEETISSFKDAGE
                     AGTHRWVAGTGGELGARARDAGIGVVGLPGFFQPRMTVGYMTVVATYIAHLAGVAPDI
                     RRELEQAADFLDGCKADMEPLARALASQIGDTPVVVHGASLTTAPARRWANQFNENAK
                     QLAFSAEIPEANHNLMEAWSKGSGGLGAVFLTDSGQSARERRRMKLTAESIEGSGAQV
                     FEVETLGETRSERLFWSVMFGDLVSVEVASLRGVDPLPVDEIQNFKRRLGNT"
     gene            complement(4004..4975)
                     /locus_tag="PLJ64_06930"
     CDS             complement(4004..4975)
                     /locus_tag="PLJ64_06930"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HNC93249.1"
                     /translation="MVLVGGKGTRLRPLTYETPKPALTLVDRPFLNYMTEWLGSHGVD
                     EVILACGFLPDELKKVLGEGEPGGPKLRYLVEEETLGTAGAIRFALDHLDERFFALNG
                     DVLADLDLTALWNQHEETGAQATLGLYEVEDSSSYGLVDIDSDGQVLKFSEKDPTRTG
                     TGLINAGTYVLEREAIAAIEPGREVSIETEFYPSITGKGLYARPLEGYWMDFGTRQRY
                     FDASWDILEGRIHTEVEKHSDGVFIAAGAEVDPTATIGPRAVIGAGAKVASGARVTGS
                     VVLEGSEIGADAVIENSIIGPGVNVAARGTVGNEVLGKNEIGETVNA"
     gene            complement(4991..6295)
                     /locus_tag="PLJ64_06935"
     CDS             complement(4991..6295)
                     /locus_tag="PLJ64_06935"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNC93250.1"
                     /translation="MNSEEKTPVGVIGVGWVGLVTAACFAELGHPVIARDVVQEKVDA
                     LSRGEITIHEPGLAELLERNRDRLTFTTDMDELLNGARLLFVCVDTPPTYSGDADLSR
                     VRSVVEDLSADGDHVLIMKSTVPAGTGASIRRELPGLAYVSCPEFLKEGTAVEDFMHP
                     DRVVVGSEPEDSAAAEEVADLYRPLGGEFVMTDTSSAEMIKLASNAYLATRISFINEI
                     ANVCEEVGADVSEVARGMGLDERIGPHFLRPGIGFGGSCFPKDVNALKLLAGNSGYHF
                     QLLNSVIEVNELQKRRVVSKLTKHLGPLVGKRIALLGLAFKPDTDDMREASSLVLSAR
                     LRGEGAEVVAYDPVAMSNARSLLPGVEMSDTAQEALAGSDAAVLVTEWSEFKEIDWTE
                     AADRMRRPLLIDGRNFLDAGQLRSAGFLYEGIGKAGTGDESG"
     gene            complement(6301..8427)
                     /locus_tag="PLJ64_06940"
     CDS             complement(6301..8427)
                     /locus_tag="PLJ64_06940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HNC93251.1"
                     /translation="MTEEQSEPEEREEPPAEEPVVEEPPAEEPVAGTPEPDPGEYADP
                     PTEELFAFEARVEEGEVPDLDPVFRAALLESSESALGEQPPSSEPPAGTGESAAAPEA
                     DQQPTTGEEQNTGEQPLTGEEPYTGEEPVTGTSEFEPPTDTGITAEQTAVLQATARSE
                     VHDAIEYSRRTGNAWDPPRPPTGGDFDKPAKRPRYWWRFLLATLLIVFSFATATQASV
                     MYFVNSIVEDLHKPNQKPLPPGILNADDGPQTIAIIGSDVRTGGGAASGDPGRSDTTL
                     LVRLDPDTNQIAMLSIPRDLYVDIPGYGYDKFNAAFSYGGTELTLKTLKSITGLDINH
                     YINVDFQGFADVIDAIHCVYVDVDRHYFNDNSQGGEEYATIDIEAGYQKLCGSDALDF
                     ARYRHTDSDLVRAARQQDLMGEVRNRLSFGELLSQRDELLKAFVDNTTTDIGETGDSK
                     DVFSLLKLLLDSRGAAVTEVTFPSTFTMRDGVSYVEATPDEIQAAVDKFLGFEDQPGP
                     VGTLDQGDGANNPARKEAQQKQQKKAARERQKALDESTTHVPSKDEDQLVDASSSGLS
                     VAQELDSEITRTDFPVYYPKRLPSGALYADGESRVYHTRDPDKNVYGAYRMVMEVQLD
                     DGLHYFGLQGLRGWTDPPILDAPHDEVEMDDRTFQVYPEGDRIKLIAWTDGDNVYWIS
                     NSLLLGLDNEQMLGMARSTRAFTPDK"
     gene            complement(8432..9580)
                     /locus_tag="PLJ64_06945"
     CDS             complement(8432..9580)
                     /locus_tag="PLJ64_06945"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNC93252.1"
                     /translation="MDELLALAEQLIGYDTSSEDGVHEAAGFIKGWLDSRGVETEQGV
                     CRGLPVITASHGDPADPTVILHGHIDVVPAGAGQFEARVEGDRLIGRGTYDMKAALAA
                     MMLTLPTEAEPLPGLRVVLGIVSDEESEEAENRGSNFLVREPDAAGPDRNGGTAATGG
                     MGLSGDFAITGEPTDMHVGVAAKGVLGLRLTVAGRAAHGSTPWLGENAVLLAVDMFRG
                     VESLPFARESSELFDRPSINLGRIWGGDALNKVPDSCAIDVDIRYLPHQDPAQIRDQI
                     AGLGPARMETIMELPPVSGGGTDSPWIQALTAAASRHHEGLSLSVGRDGASDAVSFIR
                     AGIPAVEFGPAGAGHHGPDEWVSIPSLIAYRSALKDFLKRAAKSAGRG"
BASE COUNT         1677 a         3242 c         3210 g         1841 t
ORIGIN      
        1 ctgcttggcc aggcgggcga aggtgtagta ggtctgagcc gcctcttccg gggcaccgga
       61 gatgatcagc ggggtccggg cctcgtcgat caggatgttg tcgacctcgt cgacgatcgc
      121 aaagtcgtgg gtgcgctgga ccttctgctc gagcgaggca gccatgttgt cgcgcaggta
      181 gtcgaaaccg aactcggagt tggttccgta ggtgacgtcg caggcgtact tctcgcgccg
      241 cgcggcggga tcgtcgttct cctggatcca gccgaccgac acgccgagca tgtcgtagat
      301 cggcttcatc cagagcgcgt cgcgtttgga gaggtagtcg ttgacggtga ccacgtgaac
      361 cgacttgccg ccgagggcgt tgagcacgat ggccagggtg ctggtcaggg tcttgccctc
      421 accggtcttc atttcggcga ttgcgccgtc gtggaccacc atgccgccga tcaactggac
      481 gtcgtagtgg cgctggccga tcgcccggaa ggcagcctcg cgggtcaggg caaacgactc
      541 cggaagcagg tcgtcgagcg actctccgtt cttcgccctt tccctgagcg cgttggcttc
      601 ttcaagcagc tcgctgtctt cgagctcctt catttccggc tcgatccggt tgatcgactc
      661 gacccgcttt tcgtatgcct tgaacttgcg cccctccccc atcctgagcg cgcggtcgat
      721 tatctgaatg gccatgcgac aaggttagcc gctcggcttc accgccgact aaaggcggag
      781 cccggctgca agacgaaggg gccggatatc ggcccctccg cactgactgg attgcttccg
      841 gtcccgggcc gttggccctg accggcacct ttctcggcct agaggccggg ctgcgcgcct
      901 ccggtctcac gtccctggag gcgattgaca agcttccggg tttccctccg cttccttctc
      961 ttctcccgat gccgtttcac ctggcgccgc atgtcctcgg cgagttcgtg gatggtgtgg
     1021 gtcatctccg ggctcgcctc atgggcatga agggtgactc ccttcaggtg aagcgttgct
     1081 tcggccacga acttgtcgga aatggccggg ttctgctcct cggagaaaac tacttccagg
     1141 cgggcgagcg acgaaacctg ttcaccgagc cgctcgaacc gctgggtcac ctgctcgcgc
     1201 atgtcgtcgt tgacttcgac gttgcgacca cgaatctcga ttcgcatggg acctcctggc
     1261 gggttgtgtt gctttggccg atcgtagccg ggtaacagga tgatggcaag ggcaaagtgg
     1321 cgaatttcac agccggcggg tgaaggtcag ggccgaaacc gacccggcac cggccagcag
     1381 cagcgccccc gccgcggtcg agagggttgc cccggttgtc atcacgtcat cgaccagcag
     1441 gaccgtccgg ccgccgacct ggcgaccggc agcttcggtc ggcctgatgt cgggcgggtc
     1501 ggagagccgg ccggcccggc cacggccgcg ctggcgaccg gaaccccggc gggccagcac
     1561 cggaaccggg ggttcgggaa tgtcgagcag gccggcaagc cgggcggtca gcatcgcgac
     1621 cggatcgaag ccgcgaaacc aggtccggag gctggccgga ggcacgggga caaggagcga
     1681 ctgctcgccc ggctgcccgg cggcatcggc catgtacccg gcgatcaacc cgcccagggt
     1741 cgtgagatgc ctgaacttga aggccgccag caggtccctg ggagccccgt catggtcggc
     1801 gcaggagacg atccggtcaa ccccctccgg tggctcaccc cgcagcaccg gtgagcggtt
     1861 cagctcgcgc atgcattcgc cgcagaccag caaatcgggg tcgatcgcgc cccggcagag
     1921 cgggcaacgc gaaggaacga acatcccggc aatccggtca aggtccatcc cccaaccatc
     1981 cccgaaaaaa cctcacaagt ggctcaacaa ctgcaacgat tcagtcacga gtgggaccca
     2041 acaggccgcc ggccaggaag gctccctttt ccagggatga gcggctgagc aggactgctt
     2101 cccgcacccc ggcccccgcc ccgatcgagc aaccctcgcc aaggaccaga ggcccgacca
     2161 gctgggcgcc ctcgccgatc cgggccccgt ccccgatcag gaccggcccg tcgatctcgg
     2221 cgccatcgag tccctcgact tcgcccgcga cccagacctc cggcaggggc tgcttttcgg
     2281 gcagccccag gtcaacgtcg ccaaagagtc cgtcgaagtt gctctgcacg agctcttcga
     2341 tcgtgccgat gtcattccag tacgcgtcgg tctcgtgcgc gtagaaggcg atcccggcgt
     2401 ccagcatcgc cgggaagacg tcataggccc agtcggcaaa atcgtcggga tggcccgggg
     2461 ccattagctt gctttcaccg ggaccgggaa agtagtcgaa gatctcgcgg ttgaacatgt
     2521 agatgccgca gttcgcgagg tcggagagag cctcgtcggg gtccggcttc tcctggaagc
     2581 cctggattcg cccttcatcg tcggtgatca cgacgccaaa ctcggaagtg tcatcgaccc
     2641 gtcgggtcgc gagagtcgcc atgccgccgt gtgaccggtg gaactcacgc atcgccgaaa
     2701 ggtcgatgtc ggtcatcgca tcaccggaga tgaccaggaa gtcatcgtcg aagaaggccg
     2761 aggcgttgcg tacgccgccg gcggtgccga gcagttcttc ctcgaagctg taactgagtt
     2821 cgatgccgaa acccgagccg tcgccgaagt ggtcccggat cacgtccggg taccagtgga
     2881 tgttggcgat gacttcggtg aagccgtgac gggcgaggag ccggatgatg tgctccatga
     2941 ccggccggtt caggaccggg accatcggct tgggcactgc ccgggtgact ggcctgagcc
     3001 tcgtgcccag tccggcggcc agcaccattg ctttcatcag gtattgccca ggcgccgttt
     3061 gaagttctgg atttcgtcga ccggcaaagg atcgactccg cgcagggagg caacctcgac
     3121 cgagaccagg tcaccgaaca tcaccgacca gaacaggcgt tccgatcgtg tctcgccaag
     3181 ggtctccacc tcgaagacct gggcgcccga cccctcgatc gattcggcgg tgagcttcat
     3241 ccgccgccgt tcgcgggccg actggcccga atcggtgagg aatacggctc ccagccctcc
     3301 ggaccccttc gaccacgctt ccatcaggtt gtggtttgcc tcggggatct cggccgagaa
     3361 tgcgagctgt ttggcgtttt cgttgaactg gttggcccag cgcctggccg gggccgttgt
     3421 caggcttgct ccatgcacga caacgggagt gtcccctatt tgggaggcaa gtgccctcgc
     3481 cagcggttcc atgtccgcct tgcagccgtc gaggaaatcg gccgcctgct cgagttcgcg
     3541 ccggatgtcg ggggccacgc cggccagatg ggcgatgtag gtggcgacga cggtcatgta
     3601 tccgaccgtc atccggggct ggaagaagcc gggcaggccg accacgccga tgccggcgtc
     3661 acgggcccgg gcgcccagtt cgccgccggt gcctgcaacc cagcgatggg tgcccgcttc
     3721 accggcgtcc ttgaacgagg agatcgtttc ttccgtgtcg cccgagtaac tcgaagcgag
     3781 cacggcccag tcggcggtca cccatttggg cagaccgtaa gagcgcgaaa cgaccatcgg
     3841 gccggtcagg cgattgtcga ggaccccgcg ggcaagctcg gcgccaatcg ccgatccgcc
     3901 catcccgcag accacgaagc cggctgaggg tcgagccttg agctgtgccg aatcaacccg
     3961 ccagagggca tcacgaatgt ggttggggat gtcaagtacg tcctcaagca ttgacggtct
     4021 caccgatttc attcttgcca aggacttcat ttcctaccgt accccttgcc gcaacattta
     4081 caccaggtcc gatgatcgag ttttcgatca ctgcgtcggc accgatttcg cttccttcga
     4141 ggacgaccga gccggtcaca cgggcaccgg aagccacttt ggcgcctgct cctataaccg
     4201 ccctggggcc gatagttgcg gttggatcga cctcggcgcc ggcggcgatg aagaccccgt
     4261 cgctgtgctt ttcaacctcg gtgtggatgc ggccttcgag gatgtcccag ctggcgtcga
     4321 agtagcgctg cctggtcccg aagtccatcc agtagccctc cagcggccgg gcgtagagac
     4381 ccttgccggt gatcgaaggg tagaactcgg tctcgatcga gacctcccgg cccggttcga
     4441 tcgcggcgat cgcctcccgc tccaggacat aggtgccggc gttgatcagc ccggtgccgg
     4501 ttcgcgtggg atccttctcg ctgaacttca gcacctggcc gtcggagtcg atgtcaacga
     4561 ggccgtagct gctcgaatcc tcgacctcgt agagccccag cgtcgcctgg gcgccggtct
     4621 cttcgtgctg gttccagagg gcggtcaggt cgagatcggc aagcacgtcg ccgttgaggg
     4681 cgaagaaacg ctcgtcaagg tggtcgagag cgaaacggat cgcccccgcg gtgccgaggg
     4741 tttcctcctc gaccagatac ctgagcttcg gcccgcccgg ttctccttca ccgaggacct
     4801 tcttcaattc atccggcaag aagccgcagg caaggatgac ttcgtcgacc ccgtggctgc
     4861 ccagccactc ggtcatgtag ttcagaaacg gccggtcgac cagggtcagg gccggcttcg
     4921 gcgtctcgta agtcaggggt cggagtcggg ttcccttacc gccaaccagt accaatgcct
     4981 gcaatttcgt ttacccgctt tcgtcaccgg tcccagcctt gccgattcct tcgtaaagga
     5041 acccggcgga gcggagttgg ccggcatcaa ggaagtttcg tccgtctatg agcaatggcc
     5101 tccgcatccg atcggccgcc tcagtccaat cgatctcctt gaattccgac cactcggtaa
     5161 ccagtaccgc ggcgtccgaa cctgcaagtg cctcctgggc tgtgtcactc atttcgaccc
     5221 ccggcaggag actccttgca ttactcatcg ctacggggtc atatgcaaca acttccgcac
     5281 cttcgcctct caggcgagcg gacaaaacga ggctcgatgc ttccctcatg tcgtcggtat
     5341 cgggtttgaa agcgaggccg agaagggcga tccgcttgcc gaccagcggc cccaggtgtt
     5401 tcgtcagttt gctcacgacc cgtcgtttct ggagttcgtt gacttcgatc accgagttga
     5461 gcagctggaa gtggtagccc gagttaccgg ccagtaattt cagggcattg acgtccttgg
     5521 gaaagcagga accgccgaag ccgatcccgg gacgcaggaa atgtgggccg atccgctcgt
     5581 ccagacccat cccccgtgcg acctcgctga cgtcggcacc gacctcttcg cagacgttcg
     5641 cgatctcgtt gatgaaggag atgcgggtcg ccaggtaggc attcgaggcg agcttgatca
     5701 tctcggcgct cgaggtgtcg gtcatcacga actcgccacc aagcgggcgg tagaggtcgg
     5761 cgacttcttc cgctgcggcg ctgtcctccg gctcggatcc gaccacgacc cggtcagggt
     5821 gcatgaagtc ctcgaccgcc gtgccttcct tcaggaactc cgggcaggac acgtaggcca
     5881 gccccggaag ctcgcgccgg atcgaggccc cggtgccggc cggcaccgtg ctcttcatga
     5941 tcagcacgtg atccccgtcg gcgctgaggt cctcgaccac cgagcgaacc cgggaaaggt
     6001 cggcgtcacc ggagtaggtc ggcggagtgt ccacacagac gaagaggagg cgggcgccgt
     6061 tcagcagttc gtccatgtcg gtcgtgaagg tcaggcggtc gcggttcctc tccaggagct
     6121 cggccaggcc gggctcgtgg atcgtgatct cgccgcggct tagcgcgtcg accttttcct
     6181 gaaccacgtc ccgggcgatt accgggtggc cgagttcagc gaagcatgca gcggtgacga
     6241 ggccgaccca gccgaccccg atgacgccaa ccggcgtctt ttcttctgag ttcatttcac
     6301 tcacttgtcg ggggtgaatg cccgggtcga ccgggccatg ccaagcatct gctcgttgtc
     6361 gagaccgagg agcagcgaat tggagatcca gtacacgttg tcgccgtcgg tccaggcgat
     6421 caacttgatg cggtcgcctt ccgggtaaac ctggaaggtc cggtcgtcca tctcgacctc
     6481 gtcgtgaggc gcatcgagaa tgggagggtc ggtccagcca cgcaggccct gcaggccgaa
     6541 gtaatgcaga ccgtcgtcga gctggacctc catcaccatg cggtaggcgc catagacgtt
     6601 cttgtccgga tcacgggtgt ggtagacccg gctctcgccg tcggcgtaga gggcgccgga
     6661 gggcagccgc ttcgggtagt agaccgggaa gtcggtgcgg gtgatctcgc tgtcgagctc
     6721 ctgtgccacc gagagcccgc tgctggaggc gtcaaccagc tggtcctcat ccttgctcgg
     6781 gacatgcgtg gttgactcgt ccagcgcttt ctgacgctcc ctggcggcct tcttctgttg
     6841 cttttgctgc gcttccttgc gggctgggtt attggccccg tcgccctggt cgagagtgcc
     6901 gaccggtccg ggctgatcct cgaaaccgag gaacttgtca accgcagcct gaatttcgtc
     6961 cggggtggct tcgacatagg agacgccgtc ccgcatcgtg aacgtcgagg ggaaggtcac
     7021 ctctgtcacg gcggcgcccc gtgaatcgag caggagcttc agcagcgaaa agacatcctt
     7081 gctgtcaccg gtctcgccga tgtccgtggt cgtgttgtcg acgaaggcct tgagcagctc
     7141 gtcgcgctgg ctcagcagct caccgaagct gagccggttg cgcacctcgc ccatcaggtc
     7201 ctgctggcgg gccgcccgga ccaggtcgga gtcggtgtgc cggtaacggg cgaagtcgag
     7261 cgcatcggag ccacaaagct tctggtagcc ggcctcgatg tcgatcgtcg cgtactcctc
     7321 gccgccctgg ctgttgtcgt tgaagtaatg gcggtcaacg tcgacgtaga cgcaatggat
     7381 cgcgtcgatc acgtcggcga atccctggaa gtcgacattg atgtagtggt tgatgtccag
     7441 accggtgatc gacttgagcg tcttcagcgt cagctccgta ccgccgtagc tgaaggcggc
     7501 attgaacttg tcgtacccgt atccggggat gtcgacatag aggtcgcggg ggatcgaaag
     7561 catcgcgatc tgattggtgt cggggtcgag ccggaccagc agcgtcgtat cggaacggcc
     7621 ggggtcgccg ctggccgcgc cgccgccggt gcggacgtcg gaaccgatga tcgcgatcgt
     7681 ctggggcccg tcgtcagcgt tcagaatgcc gggcggaagt ggtttctgat ttggcttgtg
     7741 gagatcctcg acgatgctgt tgacgaaata catcaccgac gcctgcgttg ccgtggcgaa
     7801 ggaaaagacg atcaggaggg tcgccaggag gaagcgccac cagtagcggg ggcgcttggc
     7861 cggcttgtcg aagtcacctc ccgtcggcgg ccggggcgga tcccaggcat tcccggtgcg
     7921 acgggagtat tcgatcgcgt catggacctc gctgcgagcc gtcgcctgga ggaccgcggt
     7981 ctgctcggcg gtgatcccgg tgtcggtggg cggttcgaac tcgctggtgc cggtcaccgg
     8041 ttcctcgccc gtgtacggct cttcgccggt cagcggctgc tcaccggtgt tttgctcctc
     8101 gccggtggtc ggttgctgat cggcctccgg ggcggctgcc gattccccgg tgccggccgg
     8161 cggttcggat gaaggcggct gttcgccgag cgcgctctcc gagctttcga gcagggccgc
     8221 ccggaagacc ggatcgagat cgggcacctc gccctcttcg acccgcgcct cgaaggcgaa
     8281 gagctcctcg gtcggagggt cggcatattc gcccgggtcc ggctcgggag tcccggcaac
     8341 cggctcttcg gctggcggct cttcgacaac cggttcttcc gctggcggct cttcccgctc
     8401 ttcgggttcg ctttgctcct cggtcatcgg atcaccccct cccggctgat ttcgcggcgc
     8461 gcttcagaaa gtccttcagg gccgaccggt aggcgatcag cgaggggatt gaaacccatt
     8521 cgtcggggcc gtggtgacca gctcccgccg ggccgaattc gacggccgga atcccggcgc
     8581 ggatgaacga aacggcatcg gaggctccat cccggccgac cgaaagggac aggccctcgt
     8641 gatgccttga ggccgctgcg gtcagagcct ggatccaggg cgagtcggta ccgccgccgc
     8701 tgaccggcgg cagttccatg atcgtctcca tccgggcagg gccgagcccc gcgatctgat
     8761 cgcgaatctg tgccgggtcc tggtgtggca ggtagcggat gtctacgtct atcgcgcaag
     8821 aatcgggaac cttgttgagc gcgtcgccgc cccaaatccg gccaagattt atcgaggggc
     8881 ggtcgaagag ctcggagctt tctctcgcaa agggtagtga ttcgaccccc cggaacatgt
     8941 ccacggcaag caaaacggcg ttttcgccca gccagggcgt cgaaccatgg gccgcccggc
     9001 cggccacggt caggcggaga ccgaggactc ccttggccgc cactccgaca tgcatgtcgg
     9061 tcggttcgcc ggtgatcgcg aagtccccgc tcaggcccat gccgccagtg gccgccgtcc
     9121 cgccgtttcg gtccgggccg gcggcatccg gctcacggac gagaaagttg ctgccacggt
     9181 tctcggcttc ttccgattcc tcgtccgaga cgatgccaag cacgacccgg agccccggca
     9241 acggctcggc ctcggtcggc agggtcagca tcatcgcggc gagcgcggcc ttcatgtcat
     9301 aggtgccgcg gccgatcagg cgatccccct cgacccgggc ttcaaactgc ccggctccgg
     9361 ccggaaccac atcaatgtga ccgtgaagga tcacggtcgg gtcggccgga tcgccatgcg
     9421 atgcagtgat caccggcagc ccgcgacaga ccccctgctc ggtctccacg ccccggctgt
     9481 cgagccagcc cttgatgaaa ccggcagcct cgtgcacccc gtcttccgaa gaggtgtcat
     9541 aaccgatcaa ctgttcggcc agggcgagca gttcgtccat cgaggaatca taagtcggcg
     9601 ggcttcaccc cgcctccccc gtcaacccgc catcccacag aagcaaccaa agtgtcgtgc
     9661 ctcctaatcg acataaggtg ccgcaaattc agccccgtga tggtgctgaa tatgcggcac
     9721 ctccgggcct gaaagtgcgg cacctccggg tctgaacggg caggcgagga gcgaagggtg
     9781 cggggccccg ggaaatcgag gagggagtta aggccacata gcgcacccaa ctccgacttc
     9841 gatccaggcc accaggccga gatcggactt gagtgcgcta tgtggcaaaa actggaccct
     9901 cacgattcgg gccaccccag gcccaggcaa cccgcaggcc agcaccgccc ccgtagcagc
     9961 aggccagcac
//
LOCUS       DAOQKN010000082         9693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_3325_length_9693_cov_5.460365, whole genome shotgun sequence.
ACCESSION   DAOQKN010000082 DAOQKN010000000
VERSION     DAOQKN010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 9693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9693
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3325_length_9693_cov_5.460365"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="PLJ64_06950"
     CDS             complement(<1..835)
                     /locus_tag="PLJ64_06950"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HNC93253.1"
                     /translation="MSIHAESRPLETPLPGGKEGATVTVEPMVTGTFSSPPILHDSPK
                     GKAGTLAILRSTRKEREPTPIPCFLIRHPSAGLILVDTGLHPSIASDPASNLGKFTTW
                     LMRPHQEPGQDVLSQLREKGINPADIRMVIFTHLHYDHASAISEFPEATLIVNDVEWK
                     YAATVRSPDFKGYRRQQFDYAFDFRTISFDSDKVSSYASFGRTLDLFGDGSIRLASTP
                     GHSPGHQSLICRLGDRDLVISGDAIYEESKLEAGANLPGTMFDEHNYKRSLSELKRFH
                     ET"
     gene            886..1734
                     /locus_tag="PLJ64_06955"
     CDS             886..1734
                     /locus_tag="PLJ64_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNC93254.1"
                     /translation="MGENVRSEIFREDLLEGKVVVLSGAGTGLGREATLEMIRLGATV
                     VGCGRRAEPLEETAKLAGNLPGAFEHDTLDIRDEEGVDSFFDGVMERHGRIDTLVNNA
                     GGQFLTPAEAITPKGFRTVIELNVTGTWLMTHAAATKAFIPQGEGAVISVTLSPHNGM
                     PGMVHSGAARAAVENMMKTLSIEWARFGIRTCAIAAGQFATDTFVNKYPKEIVDVVHR
                     TIPMGRVGESEEMGWLIAFLASGAGAFHSGSVITLDGARDNWFGPWPPPSITGESGTP
                     PAEERR"
     gene            1895..2644
                     /locus_tag="PLJ64_06960"
     CDS             1895..2644
                     /locus_tag="PLJ64_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93255.1"
                     /translation="MASASATAAARPHVVTVRAPQKMAQGSAYVVATVIRNPAAKIAR
                     GSIRITLSTNRGSGPWLLGRREVAGVAPRGRKSVLAGARIKRSTGTGKYFVVSCFRGG
                     WGSTCGATSVRVVPIATGPPGKDGTTGATGSTGATGGTGPIGPIGPTGNTGSTGPTGP
                     TGVTGFTGPTGATGATGATGVTGVTGPIGPTGATGLTGTTGSTGLTGPTGATGLTGST
                     GLTGPTGSTGLTGSTGLTGSTGFTGPTGPVG"
     gene            2675..4510
                     /locus_tag="PLJ64_06965"
     CDS             2675..4510
                     /locus_tag="PLJ64_06965"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNC93256.1"
                     /translation="MRVRRLAVMGLLAALAGCLVAAPAQAAPQPKMTAVKAPPKLAVG
                     GRYKVTSIVTNRSGSRVRGPITLTLSKDRKPGAVKLGSRRATASGKGRSRIVTRATLK
                     KSIAVGRYFLVSCFGFPGGSTCAARRVTVIKYVNGARSGGDSLFPEIGNGGYDALHYE
                     ISLDYDPLTNQFNPGTYTKMTARATEYLRQFSLDFEGPEISRIEVNRRRSNFIRRGTK
                     LIITPRIRLDAGRRFTVKVEYSGYVNHHVDPDESLEGWVRACRSGDPGPDNPDCFGSF
                     TVSEPIGAQTWFPNNNVPSDKATFTTTTAVPTEAGADEWTALGTGEFVSKQEGTDGKT
                     TWKWRESLPTSTYLTTGSVCRCVYDVASATDSSNNHTFPLYNAYDNSATPTQVDAINT
                     QLGLQEDIINEFSALFGPYPLKSGGVFAGRTSGIGYVLENQGKIHFPFPGIGNLTLAH
                     EYVHQWFGDAVGPASWSQIWFNEGWAQWGEWFHESPTTPADAFTTEYESTENPERWDI
                     PPGTLNDDPSLLFDEFSTYIRPGMMLEGYRQIVGDAKFSALAKAIQTDYAYSTIDADQ
                     FIATALEVADFGPVQEAALNDYFQQWLFLAGKPSLTPGNFPPEPL"
     gene            4649..9055
                     /locus_tag="PLJ64_06970"
     CDS             4649..9055
                     /locus_tag="PLJ64_06970"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNC93257.1"
                     /translation="MNRFAGTALLVLTILLGAVTTASAARDFWNPASSDSSQQFPLLP
                     PPLDGNVQRDDTPNDPGYDRAEPDDEDGATTTNIYDERFDLFGFPSSHTALTLYREGP
                     NALKKQISGFNAAGAWKISRGSPEVAIAVLDTGIKWDREGIRLQVRLNRNELPQPQTA
                     GGSSCAYDCNGDGAFNVADYNDDPRISHTAGPHGSAQIDAEDLITVFSDGTDSDANGY
                     VDDIAGWDFFDNDNNPYDASSYFAASGHGSGRMTEAVERGDDGQGEIGVCPGCQILPI
                     RTWDTFVADGNTVGMGMLYATDAGAKVIVAANGSIYHSAFAEAASRYAYENGVVQTYS
                     GDDLNTANHNYPANYGHAMLIQGVVTDTQGLGTDVGPELRSALCGDLLSPLGVCLGTE
                     LPVGTYFRGANTTQFGGKSSIAMEGATGSENTGKAGGAAGLVISAALQHGITLRPDET
                     RAILEQTAERVTGSALNPLSNVAGLGTADPGADPAAAREDQWTSHFGWGRVNLGEAVR
                     VASDPAATPPEAMIDSPDWYAPINSDSIELTGLARARAATGTSFSWRLEWGPGQAPTA
                     WNEVASGTGSGSGITDLGSLDMNEVRDAMASYDAPADSGGPTFSTSGANPHDHEFTVR
                     LVVTGSGIATPGIDRRVFTLADDPDLRDGFPKRLGTGGEAPVRYADLDQDGVQELIVP
                     LMDGTVHAFRPDGSELPGWPVETRLMRNADGHAAADGFAALAGETPPREPPRGPAVAD
                     IDGDGAPEVVTTAGIRVYAWESSGEEVTGFPVTSDLGKCGAALESQPLSHPKCGFIAS
                     PAIARLQGPDAAPDIVVPGLDGYLYAFSGQGDAVPGYPVRLVDPDIPAGEQMLAESIN
                     SPAVGDLNGDGRDDAVVATNETYDAQPPDFESISGGVVGSALTDILANAAGGSERVYA
                     VDSRGNGFEGNGGSPYLDGWPIKLNGAIQSTLPLIGPGQDPAITMVGGEPRVVASATG
                     SATIGIYKPDGTVALNVEQAVPGAASVADGAIGTGAVNLFESASLGKLTPGATNPSIV
                     KYGLGLPGILNLLLVGQNLPYNHLIGAYDTATGLSAPSFPRVTEDFQFLSSSTIANLN
                     GSQLSNQVLAGTGLGLINAYDGLTGLNVDGFPKVTGGWLFAPPTVSDDGRMAAITREG
                     YLFEWSLPDIPACQTEWPSFRHDPQQSGNYDRDGSSPGGLGDVAEEGGTLGFTATGDD
                     GECGVADHYEVVTSDAPVTGESFAAAQPVTGAPAPAAAGTGQSFVLPETARYLAIRAV
                     DEAGNVGPMSVVDRGDTGPTGPTGPTDPTGPTGPTDPTGPTGPTDPTGPTGPTDPTGP
                     TGPTSPTGPTGPTSPTGPTGPTGPTGPSGPDRKPPKNVKPGLAFLYDGRLYLRVHCPP
                     RFRPACRVVAKVVTKRKRGRAMTRSLRVVVRSGHWRRAALEVRPKFKARLDRLSRIKR
                     RTVVLKMKVRARRITRASHRTVIYHRLRVRRP"
     gene            complement(9109..>9693)
                     /locus_tag="PLJ64_06975"
     CDS             complement(9109..>9693)
                     /locus_tag="PLJ64_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNC93258.1"
                     /translation="VVNNAGYTWDGVAHKMSDDQFQAMLDIHTIVPFRVARALAPHWR
                     EAAKAEAAEGNEYFRKLINISSISGTMGNAGQVNYSAGKSAVVGMTKTLAKEWGQFKI
                     NVNAVAFGFVETRLTAAKEGDAETIKAPDGEEIQLGIPEQMRAMASMIIPLGRPASPE
                     EAAGPVLFLGSNLSDYVHGQVLNVTGGQFGGMYT"
BASE COUNT         1701 a         3269 c         3203 g         1520 t
ORIGIN      
        1 aggtctcgtg gaagcgcttc agctcgctga ggctgcgctt gtagttgtgc tcgtcgaaca
       61 tcgtgcccgg caggttcgct ccggcttcga gcttgctttc ttcatagatc gcgtcgccgc
      121 tgatcaccag atcccggtcc cccaggcggc agatcagcga ctggtggccg ggtgagtgac
      181 ccggagtcga ggccaggcgg atcgacccgt ccccgaaaag gtcgagggtg cggccgaagc
      241 ttgcgtacga gctgaccttg tcggaatcga aggagatcgt ccggaagtcg aaggcgtaat
      301 cgaactgctg gcggcggtaa cccttgaagt ccgggctgcg gacggtcgcc gcatacttcc
      361 actcgacgtc attgacgatc agggtcgctt ccgggaattc ggagatcgcc gaggcgtggt
      421 catagtgaag gtgagtgaag atcaccatcc ggatgtcggc ggggttgatg cccttctctc
      481 gcagctggga gagaacgtcc tgaccaggct cctgatgagg gcgcatcaac caggtcgtga
      541 acttgccgag gttggaagcc gggtcggaag cgatcgacgg gtgaagtccc gtgtcgacca
      601 ggatcagtcc ggcggaaggg tggcgaatca ggaaacaggg aatcggggtc ggttcccgtt
      661 ccttgcgggt cgatcggagg atggccagag tccccgcctt gcctttcggg ctgtcgtgaa
      721 ggatcggcgg gctgctgaag gtgcccgtca ccatcggctc gaccgttacg gtcgcgccct
      781 ccttcccgcc gggaagcggc gtttcgagcg gtctcgactc agcgtggatg ctcacctgcc
      841 ggaccttacc gccggacggc tcagggatag gattcgcggc agccaatggg agaaaacgta
      901 cgctcagaga ttttccgtga agacctgctc gagggcaagg tggtcgtgct ctccggagcc
      961 ggaaccggcc tcggccgtga agccaccctg gaaatgatcc ggctgggggc gaccgtggtc
     1021 ggttgcggcc gacgagcgga gccgcttgaa gagaccgcga agctggccgg gaatctgccg
     1081 ggcgccttcg aacacgacac ccttgacatc cgcgacgagg aaggagtcga ttccttcttt
     1141 gacggtgtga tggagcgtca cggccggatc gacaccctgg tcaacaacgc cggcggccag
     1201 ttcctgaccc cggccgaggc gatcaccccc aagggctttc gcacggtgat cgagctgaac
     1261 gtgaccggca cctggctgat gacccacgcc gccgcgacca aggccttcat tccccagggg
     1321 gaaggtgcgg tgatcagcgt caccctgtct ccccacaatg gcatgcccgg catggttcac
     1381 tcgggggccg cccgggccgc ggtggagaac atgatgaaga cgctttcgat cgaatgggcg
     1441 agattcggca tcaggacctg cgcgatcgct gccggtcagt tcgccaccga caccttcgtg
     1501 aacaagtacc cgaaggagat cgtcgatgtg gttcaccgga ccatcccgat gggacgggtt
     1561 ggggaatccg aggagatggg ctggctgatc gccttcctcg cctccggtgc cggtgccttc
     1621 cactccgggt cggtgatcac cctggacggc gcccgcgaca actggttcgg gccctggccg
     1681 ccgccctcga taaccggcga gagcggcacc ccgccggccg aagaacgccg ctaacgctcc
     1741 cgaatcgccc ggaaccgatg tggccggcgt tatgctgatc acgaaggctg gcagaggcgc
     1801 gggaaggagc tggacgatgg acttcaacga agcacggacc aggaggccga tcgggtggtc
     1861 tgttgtgctg acgctcctgt tgctgttcgg agcgatggcc tccgcctctg cgacggctgc
     1921 ggccagacca cacgtggtca cggtccgggc accacagaag atggcccagg gaagtgccta
     1981 tgtcgtggcc acggtgattc gcaacccggc cgcgaagatc gcccggggtt cgatcaggat
     2041 cacgctctcg acgaatcgcg gttcgggccc ttggctgctt ggccgccgcg aggtggccgg
     2101 ggtcgcgccg agaggaagaa aatccgttct ggccggggcc cggatcaagc gctcgaccgg
     2161 gaccgggaag tacttcgtcg tttcctgttt ccgcggtgga tggggtagca cctgtggcgc
     2221 gacatccgtc cgggtcgtgc cgatcgccac cggcccgccc ggcaaggacg ggacgaccgg
     2281 cgccaccgga agtaccgggg cgacgggcgg caccgggccg atcggaccga tcgggccgac
     2341 cgggaacacc ggctcgactg ggcccaccgg gccgaccggg gtgacgggct tcaccggacc
     2401 gaccggggcg acgggggcca ccggggcgac aggcgtcacg ggtgtcaccg gaccgatcgg
     2461 accgaccggc gccaccgggt tgaccggtac gaccggctcg acgggtctga ccggaccgac
     2521 cggggcaacc gggctgacgg gctcgaccgg tctgaccgga ccgaccggct cgaccgggtt
     2581 gacgggctcg accgggttga cgggctcgac cgggtttacc gggcccacag ggccggtcgg
     2641 ctagatgtcc gggccggacc cggaaggcag aaggatgagg gtccgcaggc tggcagtcat
     2701 ggggttactg gccgcactgg ctggctgcct tgtggccgcg ccggcccagg ccgcaccgca
     2761 gccgaaaatg acggcggtaa aggcaccgcc gaagctggcg gtgggcggcc ggtacaaggt
     2821 gacctcgatc gtgaccaacc gttccggatc cagggtccgg ggaccgatca ctctgaccct
     2881 ttcgaaagac cggaagccgg gcgccgtcaa gcttggatcc cgcagggcga ccgcgagcgg
     2941 gaaaggcagg agccggatcg tcacgcgcgc caccctgaag aagtcgatcg ccgtcggcag
     3001 gtacttcctc gtctcctgtt tcgggttccc ggggggatcc acctgcgccg ccaggcgggt
     3061 caccgtcatc aagtacgtaa atggcgcccg cagcggcggg gattcgctct ttcccgagat
     3121 cggcaacggc ggctatgacg cgctccacta cgagatcagc ctcgactatg accccttgac
     3181 caaccagttc aacccgggca cctacacgaa gatgaccgcc cgggccacgg aatacctgcg
     3241 gcagttcagc ctcgacttcg agggccccga gatctcccgg atcgaagtca accgaaggcg
     3301 ctccaacttc atccggcggg gcacgaaact gatcatcacc ccgcggatcc gcctggacgc
     3361 cggccgcagg ttcacggtca aggtcgagta ctcgggttac gtcaaccatc acgtcgaccc
     3421 cgacgaatca ctcgaaggct gggttcgcgc ctgtcgctcg ggggatccgg gtcccgacaa
     3481 cccggactgt ttcggctcct tcacggtgag cgagccgatc ggcgcccaga cctggttccc
     3541 gaacaacaac gtcccgtctg acaaggcgac cttcaccacc acgaccgcgg taccgacgga
     3601 agccggcgcc gacgaatgga ccgcgctggg gacgggggaa ttcgtctcga agcaggaagg
     3661 aaccgacggc aagacgacct ggaagtggcg agaatccctg ccgacctcga cctacctgac
     3721 caccggctcc gtctgtcgct gcgtctatga cgtcgcctcg gcgaccgaca gctcgaataa
     3781 ccacaccttc ccgctctaca acgcctacga caactcggcc acgccgaccc aggtcgatgc
     3841 gatcaacacc cagctcggat tgcaggaaga catcatcaac gagttctccg ccctgttcgg
     3901 gccctacccg ctgaagtccg gaggagtctt cgccggccgg acttccggaa tcggctacgt
     3961 gctggaaaac cagggcaaga ttcacttccc gttccccggg atcggcaacc tgaccctggc
     4021 ccacgaatac gtccaccagt ggttcggcga tgcggtcggc cccgccagct ggagccagat
     4081 ctggttcaac gagggctggg cccagtgggg cgagtggttc cacgagtcac cgaccacacc
     4141 ggccgacgcc ttcacaaccg agtacgaatc gaccgagaat ccggagcggt gggacatccc
     4201 gcccggaacc ctgaatgacg atccgtccct gctgttcgac gaattctcga cctacatccg
     4261 gccgggaatg atgctcgagg gctatcgcca gatcgtcggt gatgcgaagt tctccgcgct
     4321 cgccaaagcc attcagacgg actacgcata ctcgacgatc gacgcggacc agttcatcgc
     4381 cactgccctc gaggtcgccg acttcgggcc ggtacaggaa gcggccctga acgactactt
     4441 ccagcagtgg ctgtttctcg cgggcaaacc ttcactgact ccgggcaact tcccgccgga
     4501 acctctttag gcgccgttcg cgggtcgccg gccagacccg cctccttccg caccgggttt
     4561 acggaaggaa gggctccggc ctgtttgaga accggcggat ctgcacataa aggttccagt
     4621 actcgtctac caaaccggga gcttgggagt gaacagattc gccggcacag ccctgctggt
     4681 cctgaccatc ctgctgggag cggtaaccac tgcctcggcc gcccgtgact tctggaaccc
     4741 cgcatcgagt gacagctcgc agcagttccc gcttctgccg ccgccgctcg acggcaacgt
     4801 gcagcgcgat gacaccccca atgacccggg ctacgaccgc gccgagccgg acgatgagga
     4861 cggcgcgacg accacgaaca tctacgacga gcgcttcgat ctcttcggct ttccttcctc
     4921 ccacacggcg ctgactctct accgcgaagg gccgaacgcg ttgaagaagc agatctccgg
     4981 cttcaacgcg gccggcgcct ggaagatcag tcgcggcagc cccgaggttg cgatcgcggt
     5041 cctcgacacc ggcatcaagt gggaccggga gggaatccgg cttcaggtcc ggctgaatcg
     5101 caacgagctt ccccagcccc agactgcggg cggttcgagc tgtgcctacg actgcaacgg
     5161 cgacggggcc ttcaacgtcg ccgactacaa cgacgatccc cggatttcgc acaccgccgg
     5221 ccctcacggc tcggcccaga tcgacgccga agacctgatc accgtcttct cggacggaac
     5281 ggattcggat gcgaacggat acgtcgatga catcgcgggc tgggacttct tcgacaacga
     5341 caacaacccc tacgacgcgt cgagctactt tgcggcgtcc ggtcacggct ccggccggat
     5401 gaccgaagcg gtcgagcggg gcgacgacgg ccagggggag atcggggtat gtccgggctg
     5461 ccagatcctg ccgattcgta cctgggacac cttcgtcgcc gacggaaaca cggtcggcat
     5521 gggcatgctc tacgcgaccg acgcaggcgc gaaagtgatc gtggccgcca acggttcgat
     5581 ctaccactcg gccttcgccg aagccgcttc aaggtacgcc tacgaaaacg gcgtggtcca
     5641 gacctactcc ggtgacgacc tcaacaccgc caaccacaac taccccgcca actacgggca
     5701 tgcgatgttg atccagggcg tcgtcacgga cacccagggt ctcggcaccg acgtcgggcc
     5761 ggagctccgc agcgcgctct gcggcgatct gctctcgccg ctgggcgtct gcctgggtac
     5821 cgagcttccg gtcgggacgt acttccgcgg ggcgaacacc acccagttcg gtggcaagag
     5881 ttcgatcgcg atggaaggcg cgaccggttc ggaaaacacc ggcaaggccg gtggtgcggc
     5941 agggctcgtg atcagcgccg ccctccagca cgggatcacc ctgagaccgg acgagaccag
     6001 ggcgatcctg gaacagaccg ccgaacgggt gaccggttcc gccctcaacc cgctcagcaa
     6061 cgtggccggg ctggggacgg cggaccccgg cgccgacccg gcggcagccc gcgaagacca
     6121 gtggacctcc cacttcggct ggggccgcgt caacctgggc gaggcggtca gggtcgcgtc
     6181 cgatcctgcc gcgactccgc ccgaggcaat gatcgattcc cccgactggt acgcgccgat
     6241 caactcggat tcgatcgaac tgaccggcct cgcccgcgcc cgggcagcga ccggaaccag
     6301 tttcagctgg cgcctcgaat ggggtcccgg ccaggcgccg accgcctgga acgaagtcgc
     6361 ctccggtacc ggcagcggct cgggaatcac cgaccttggc agtctcgaca tgaacgaggt
     6421 gcgggatgcc atggcgagct acgacgcacc ggctgattcc ggcggcccga ccttctcgac
     6481 ttccggcgcg aacccgcacg accacgagtt cacggtccgg ctggtggtga ccgggtccgg
     6541 gatcgcaacc cccggcatcg accgtcgcgt gttcaccctg gccgatgacc cggacctgcg
     6601 tgacggcttt ccgaagcggc tcgggaccgg cggcgaagca ccggtccgtt acgccgacct
     6661 cgaccaggac ggggttcagg aactgatcgt gccgctgatg gacggcacgg tccacgcgtt
     6721 ccgaccggac ggatccgagc tgccgggctg gccggttgag acgcggctga tgcgaaacgc
     6781 cgatggtcac gccgccgccg acggtttcgc ggccctggcg ggcgagacgc cgccccggga
     6841 gcctccccgc ggcccggcgg tggcagacat cgatggcgac ggcgcgccgg aagtggtgac
     6901 gacggccgga atcagggtct acgcctggga atccagcggt gaagaagtca ccgggttccc
     6961 ggtcacgtcg gacctcggca agtgcggtgc cgcgctcgaa agccaaccgc tttcgcatcc
     7021 caagtgcgga ttcatcgcca gccccgcgat cgcgcgactt cagggtccgg acgcggcgcc
     7081 cgacatcgtc gtccccgggc tcgacggtta cctctacgcg tttagcggcc agggcgacgc
     7141 cgttccgggg tacccggtgc gtctggtcga ccccgacatc ccggcgggcg agcagatgct
     7201 ggccgaatcg atcaactcgc ccgcggtcgg tgacctgaac ggcgacggtc gcgacgatgc
     7261 cgtggtcgca accaacgaga cctatgacgc ccagccgccg gatttcgaga gcatctccgg
     7321 cggcgtggtc gggtcggccc tcaccgacat cctcgccaac gcggcgggag gttccgagcg
     7381 ggtctacgcc gttgactcac gcggcaacgg ttttgaaggc aacggcggaa gcccctatct
     7441 ggacggctgg ccgatcaagc tgaacggcgc gatccagtcc accctgcccc tgatcgggcc
     7501 gggacaggat ccggcgatca cgatggtggg cggtgaaccg cgggtcgtcg cgtcggccac
     7561 gggttcggcc acgatcggca tctacaagcc ggacggcacc gtggccctga acgtcgagca
     7621 ggcggtcccg ggagcggctt cggtggccga cggggcgatc ggaacgggtg cggtcaatct
     7681 cttcgaatcg gcttcgctcg ggaagctcac gccgggcgcc acgaatccga gcatcgtcaa
     7741 gtacggtctc ggcctgccgg gcatcctgaa cctgctgctg gtcggccaga acctgcccta
     7801 caaccatctg atcggggcct acgacacggc gaccggcctt tcggccccgt ccttcccccg
     7861 ggttaccgag gacttccagt tcctttcctc ctcgacgatc gccaacctga acggcagcca
     7921 gctttccaac caggtgctgg ccgggaccgg gctcggcctg atcaacgcct acgacggact
     7981 gaccggcctc aacgtcgatg gcttcccgaa ggtgaccggc ggctggctct ttgctcctcc
     8041 gaccgtctcc gacgacggca ggatggccgc catcacccgc gagggctatc tcttcgagtg
     8101 gtccctgccg gacatcccgg cctgccagac cgagtggcca tccttccgtc acgacccgca
     8161 gcagagtgga aactacgacc gtgacggctc aagccccggc gggctcggtg acgtcgccga
     8221 ggaaggcgga acgctcggct tcaccgcgac cggagacgac ggcgagtgcg gggtcgccga
     8281 tcactacgag gtggtcacct ccgacgctcc ggtcaccggt gaatccttcg cggcggccca
     8341 accggtcacc ggagctccgg ccccggcggc ggccgggacc gggcagtcct tcgtcctgcc
     8401 cgagaccgcc agatatttgg cgatccgggc ggtcgatgaa gccggcaacg tcggtccgat
     8461 gtcggtggtc gaccgcggtg ataccgggcc gaccggacca accggtccca ccgacccgac
     8521 cggaccgact ggcccgaccg atcccacggg acccaccgga ccgaccgatc cgaccggtcc
     8581 gacgggccca accgacccga ccggtccaac cgggccgacc agtcccaccg gtccgacggg
     8641 cccgacttcc cccacaggac ctacggggcc gaccgggcca accggtccgt caggtccgga
     8701 ccgcaagccc ccgaagaacg tcaagcccgg gctggccttc ctctacgacg gccggctcta
     8761 cctgagggtt cactgcccgc cgcgcttcag gcccgcctgc cgggtcgtgg cgaaggtggt
     8821 cacgaagcgg aagcggggca gggcgatgac gcgttcgctg agggtggtgg tccgatccgg
     8881 tcactggagg cgggcggccc tcgaggtcag gccgaaattc aaagcgcgcc tcgaccgcct
     8941 ctcgaggatc aagcgccgga cagtcgtgct gaaaatgaag gtccgggcaa ggcggatcac
     9001 ccgggccagc caccggaccg tcatctacca ccgtctccgc gtccgcaggc catgaaaaag
     9061 gagcccggtt tcccgggccc ccttggcaac tcgaaagcct gaggttcctc aggtatacat
     9121 gccgccgaac tggccgccgg tcacgttgag gacctggccg tgaacgtagt cggagaggtt
     9181 cgagccgagg aagaggaccg gtccggccgc ttcctcgggt gaggccgggc gacccagcgg
     9241 gatgatcatc gaggccatgg cccgcatctg ctccgggatg ccgagctgga tttcctcgcc
     9301 gtcgggcgcc ttgatcgtct cggcgtcgcc ttccttggcg gcggtcaggc gggtctcgac
     9361 gaagccgaaa gcaaccgcgt tgacgttgat cttgaactgg ccccattcct tggccagggt
     9421 cttggtcatg ccgaccacgg ccgacttgcc ggcgctgtag ttgacctggc cggcgttgcc
     9481 catcgtgccc gagatcgaac tgatgttgat cagcttgcgg aagtactcgt tgccttcggc
     9541 ggcttcggcc ttggccgctt cacgccaatg cggtgccagg gcacgggcga cccggaaggg
     9601 gacgatcgtg tggatgtcga gcatcgcctg aaactggtca tcagacatct tgtgggcgac
     9661 tccgtcccag gtgtagccgg cgttgttgac cac
//
LOCUS       DAOQKN010000083         9680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_3331_length_9680_cov_5.745766, whole genome shotgun sequence.
ACCESSION   DAOQKN010000083 DAOQKN010000000
VERSION     DAOQKN010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 9680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9680
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3331_length_9680_cov_5.745766"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PLJ64_06980"
     CDS             <1..206
                     /locus_tag="PLJ64_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93259.1"
                     /translation="GPQAWKVAINKFDARGNQSRFVRSYVLRGNQIAFLYYGPLAVGK
                     RAYVVIASPNGNKVKLLKLKRSQ"
     gene            complement(291..1886)
                     /gene="purH"
                     /locus_tag="PLJ64_06985"
     CDS             complement(291..1886)
                     /gene="purH"
                     /locus_tag="PLJ64_06985"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNC93260.1"
                     /translation="MDNMDPTNGPVKVRRALISVSDKTGLADFAKGLADLGVEILSTG
                     GTARALRDAGLTVIDVSEFTGQREIMDGRVKTLHPKIYAGLLARRSDPAHMEVLTEEA
                     IPPIDLVCVNLYPFEQTIAREDVGPQEAIENIDIGGPTMIRAAAKNHEDVVVLVKPEC
                     YDAVIEEMNQTEGEVSASTRHWLANEAFAQTARYDAAISAWFSESYEAFPEHRVLAFE
                     KQLDLSYGENPHQRAALYSQVGLDSNVLTGMSKHHGKALSFNNVLDLDACRKLLADLE
                     GPACVIVKHNNPCGVALGDSGLDAYERARDCDPVSAFGGVIAINQVIDVPLAEALHKN
                     FVEVLVAPGYEPEAMEILQQKENIRIFTDEAPPFPDSREKDIKRVRGGILIQDRDGEV
                     EQREMMEVVTKAQPSEQAWIDMLFAWTVCRHVKSNAIVFAKDGVTLGIGAGQMSRVDS
                     SRIAIEKCRDAHGDEADDLLAGCAMASDAFFPFADGPMVGIEAGATSVIQPGGSKRDG
                     EVIEACDETETAMVFTRHRHFRH"
     gene            complement(1931..2569)
                     /gene="purN"
                     /locus_tag="PLJ64_06990"
     CDS             complement(1931..2569)
                     /gene="purN"
                     /locus_tag="PLJ64_06990"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HNC93261.1"
                     /translation="MTPSSPFRVVVLASGGGTNLQAVLDQLHGRGEVEVVGVGSDKPA
                     AYALERATAAKVPTAVFPRDEYEDREARDAAMADWIESTGAELVALAGYMQLVSASFV
                     ERFRGQIVNVHPALLPSFPGLDAIGQALEQGVKVTGVTVHFVDEGTDTGPVIIQRAVE
                     VPANWDREELEVAIHAIEHEIYPEAIGMIARGEVKIADDEPRKVIVTQTRGK"
     gene            2682..3092
                     /locus_tag="PLJ64_06995"
     CDS             2682..3092
                     /locus_tag="PLJ64_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93262.1"
                     /translation="MILSLLLVAAVCPSPAEAIQYFGKKPGKWEAEIEIRNGFIRSMH
                     LKLPSNCRDQERPPGLDLEINRDSKLRLRRYGGFYRESVTRWSKSVLKGRVIGNRVQG
                     SLWFADLGDYVECWTGTGEKPSWVRFAARAVPPK"
     gene            complement(3245..4690)
                     /gene="purF"
                     /locus_tag="PLJ64_07000"
     CDS             complement(3245..4690)
                     /gene="purF"
                     /locus_tag="PLJ64_07000"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNC93263.1"
                     /translation="MTERIDLDREGPRDECGVFGVYAPGHDVSRLAYFSLYALQHRGQ
                     ESAGIATCEGGHITTMRDGGLVSQVFDEDKLRALTGDMAIGHVRYSTTGGGSWENAQP
                     IWRDDGRELALAHNGNLTNAVEIYNRLRRKGLEFRGTTDSEIIAALISSDYGRHIEDA
                     VANVMPQLEGAYSTVVMTKHAIVAFRDPFGIRPLSLGQLPDGSYVFASESCAFDIIGA
                     KLVREVSPGEMISLSERGLESRQVIKSERTAMCIFEHIYFSRPDTKLEGKRLQEVRGR
                     MGEILWREAPAEADLVIAVPDSGTPAARGYARASGLPQDDGLIKNRYVARTFIQPGQE
                     LRKHGLRMKFNPLPEIISGQRVVVVDDSIVRGNTTRQIVGMLRDAGAKEVHLRISAPP
                     IRYGCNYGVDMSAREEMVAHERTIEQIAEHIGADSLAYLSMEGVYEAVGTPAEVHCDA
                     CFTGNYPLGDDPEEADGKFDLEQIAVIPATR"
     gene            4848..5864
                     /locus_tag="PLJ64_07005"
     CDS             4848..5864
                     /locus_tag="PLJ64_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93264.1"
                     /translation="MAVMRGQRGLGLLVVGAILASALVLLPATSPAATKKQKQCQRAI
                     ATVFTFKKGVPKKKRRKAVKIICQTMTVGKQGPKGAKGDPGARGATGATGPKGATGSA
                     NGVTGPVGPTGATGQKGATGAIGLPGITGATGATGFQGLIGPTGPIGLPGLTGATGAT
                     GLIGEIGPTGPTGLTGLIGPTGPTGLTGAVPILISDIPSFSAQAMSDVINPITNWNED
                     YDSTNSFNPVTGVFTAPTTGSYLIEPDITTGPASEVIAGGGQPPLVAVNVNNVDVDVQ
                     AFPLFNVDINLILALDVPLQTAQASPTTFQQLDAGDQVSIQIIKQNGVVYDTYGDLMI
                     TRLP"
     gene            complement(5867..7738)
                     /gene="nhaA"
                     /locus_tag="PLJ64_07010"
     CDS             complement(5867..7738)
                     /gene="nhaA"
                     /locus_tag="PLJ64_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HNC93265.1"
                     /translation="MKPLHDPDTTESRRDLAAQVAKPMRQFLATESGSAGLLLLAGVA
                     ALIWSNAPFSDSYFSFWSTELSVKVGDLSIAHDLRYWIDEALMAMFFFVIGMEVRREM
                     AMGELTDRKRVLIAVMGGIGGMIFPALIYLAINSGSGHEHGWGMVIATDTAFVLGALA
                     IVGPKFPTQLRVFLLTLAIVDDIVAITVIGLFYSEALDYTAIAICVAALFLIWLYPRI
                     GVWRSGIYFATVGVAVIAAMESGFHATLAGMIAGLLVAAYPPQPKKVEKAARRVRAFR
                     QSPLPEVARSANLSVQQALSPNERFQTVFHPFTSYVVVPLFALANAGVDLGGGVLGDA
                     LGSTLTWGVVAGLVLGKTLGITTGVVTAIRTRIGEMPVGIGKGQTVGGAALSGIGFTV
                     SLLIARLAFDDQQTQDHAVVGIFLASIISVALAWTIFKLAAVFRGEVSAGLPRKLSLP
                     FDPDRDHFLGSPDAPFVLVEYGDYECPFCGRATGMVRELRERMGDQLLYVYRHLALVD
                     VHPHAELAAEAAEAAGEQGKFWEMHVELYDHQGDLDIEDLIGYAAKLDMDVDRFVEDV
                     QAGRLARRVQQDAASGDASGAHGTPTFFVNGVRHVGAYDAVSLAAELRAAEPVPAGA"
     gene            complement(7865..>9680)
                     /gene="purL"
                     /locus_tag="PLJ64_07015"
     CDS             complement(7865..>9680)
                     /gene="purL"
                     /locus_tag="PLJ64_07015"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HNC93266.1"
                     /translation="ERTHYLVDGVVRGIGHYGNSMGIPTVGGEIYFEPPYEQNPLVNA
                     MCVGLARTDEMVRAAAAGAGNKVVLFGSLTGRDGIGGASVLASAELDEGDDAKRPTVQ
                     IGDPFEESKLLECCQELLAEDLLVSLQDLGAAGLTSSAGEMASAGGTGIDIDVGLVPL
                     READMEPFEIMVSESQERMLAVVEPGKVEQVLALCTKWQTASAVIGEVTDSGTFRILK
                     DGETVGEIRVEDLVDGCPLYDLDPGDPGEWTYPNRETIPADASASDIALALLASPSIA
                     SKRWAFEQYDSIVGSRTAHRAGAADAAVLNIPESGRAIAVAIDGNGRRVACDPYQGTI
                     EATLECAANLAVVGAEPLGVTNCLNFGNPEKPTVAWQLGRSIDGLAEACRALEAPVVG
                     GNVSLYNETPQGPIYPTPVVGMVGELPDASRVAGEPWREGDAIALVGPFDPSLAGSEL
                     EKQRGQLGKGLPEFEIAPVRQAIELVREAVRSGSIRTATDVSDGGIATALAELAIGSG
                     VGCDAHLTALLAKRDCSAEDALFGEGPGGFILAGEEAGLEKLGGRGVEIEVIGEVRGN
                     EVIVTASDRRVEILVSEAEAAFESLEERIESDQAGITH"
BASE COUNT         1644 a         3164 c         3269 g         1603 t
ORIGIN      
        1 ccggtcccca agcctggaag gtggcgatca acaagttcga cgcccgtggc aaccagtccc
       61 gattcgtccg cagctacgtc ctgcgcggca accagatcgc cttcctctac tacggtccgc
      121 tcgcagtcgg caagcgcgcc tacgtcgtga tcgcctcccc caacggcaac aaagtcaagc
      181 tcctgaagct caagaggagt caataacaga gcccctccca accatccttt tcttcgagtg
      241 ggaggggcgg gttgagatgg gagcgcgggc aaagacccgc gcgactgcag ttagtgacgg
      301 aaatgtctgt ggcgagtgaa gaccatcgcg gtctccgttt cgtcgcaggc ctcgatcact
      361 tcgccgtcgc gctttgagcc gccgggctga atgaccgagg tcgccccggc ttcgatgccg
      421 accatcgggc cgtcggcgaa ggggaagaag gcgtcggagg ccatcgcgca accggccagc
      481 aggtcgtcgg cctcatcgcc atgggcgtcg cggcacttct cgatcgcgat ccggctcgaa
      541 tcgacccggc tcatctgacc ggctccgatc ccgagggtca ccccgtcctt cgcgaagacg
      601 atcgcattcg acttgacgtg gcgacagacg gtccaggcga agagcatgtc gatccaggcc
      661 tgctcggaag gctgcgcctt ggtgaccact tccatcatct cccgctgttc gacctccccg
      721 tcgcggtcct ggatcaggat gccgccgcgg acccgcttga tgtccttttc gcgtgagtcg
      781 gggaagggcg gggcctcgtc ggtgaagatg cggatgttct ccttctgctg gaggatctcc
      841 atcgcttccg gctcgtaacc aggggcgacc agtacctcga cgaagttctt gtgaagggcc
      901 tcggccagcg gcacgtcgat cacctggttg atcgcgatca ccccgccgaa ggcggagacc
      961 gggtcgcagt cgcgggcccg ttcgtaggcg tcgagcccgg agtcacccag ggcgaccccg
     1021 caggggttgt tgtgcttgac gatcacgcag gccgggcctt cgaggtcggc cagcagcttg
     1081 cggcaagcgt cgaggtcgag cacgttgttg aaggagagcg ccttgccgtg gtgcttcgac
     1141 atcccggtca gcacgttgct gtcaaggccg acctgcgagt agagcgcggc ccgctggtgc
     1201 gggttctcgc cgtaggaaag gtcgagctgc ttctcgaagg ccagcacgcg gtgctcgggg
     1261 aaggcttcgt aggactcgga aaaccaggcc gagatcgcgg cgtcgtaacg ggcggtctgg
     1321 gcgaaggctt cattggcgag ccagtgccgg gtcgaggcgg agacctcgcc ttcggtctgg
     1381 ttcatctcct cgatcaccgc gtcgtaacac tcgggcttga ccagcaccac cacgtcctcg
     1441 tggttcttgg ccgcggcgcg gatcatggtc gggccgccga tgtcgatgtt ctcgatcgcc
     1501 tcctgcgggc cgacgtcctc gcgcgcgatc gtctgctcga aggggtagag gttgacgcag
     1561 accaggtcga tcggcgggat cgcctcctcg gtcaggacct ccatgtgggc cgggtccgag
     1621 cggcgggcga gcaatccggc gtagatcttc gggtggagag tcttgacccg gccgtccatg
     1681 atttcgcgct ggccggtgaa ctcggaaacg tcgatcacgg tcagcccggc gtcgcgcagg
     1741 gcccgggcgg tgccgccggt ggagaggatc tcgaccccga gatcggccaa ccccttggcg
     1801 aagtcggcaa gcccggtctt gtccgagacc gagatcaggg cgcgcctgac ctttaccggt
     1861 ccgttggtcg gatccatgtt gtccatatca accctcttgt tcgtttcgaa gcgcccgggg
     1921 acggccggcg tcacttccca cgtgtttgcg tgacgatcac cttgcgaggt tcgtcatcgg
     1981 cgatcttaac ttcgccccgg gcaatcatgc cgatcgcctc ggggtagatc tcatgctcga
     2041 tcgcgtggat cgccacttcg agttcctcac ggtcccaatt tgcgggaact tccaccgccc
     2101 gctggatgat caccggcccg gtgtcggtgc cttcgtcgac gaagtggacg gtcaccccgg
     2161 tcaccttaac gccctgctcg agcgcctgcc cgatcgcgtc aagtccgggg aaggagggaa
     2221 gcagagccgg gtggacgttg acgatctgcc cccggaaccg ctcgacgaag gacgccgaga
     2281 ccagctgcat gtatccggcc agggcaacca gttcggcccc ggtcgactcg atccagtcgg
     2341 ccatcgccgc atcccgggcc tcccggtcct cgtactcgtc ccggggaaag accgcggtcg
     2401 gcaccttcgc cgcggtcgcc cgttccagcg cataggccgc cggcttgtcg gaaccaaccc
     2461 cgaccacctc gacctcgccc cgcccgtgca gctggtcaag aacagcctga aggttggtcc
     2521 ccccaccgga ggccagcacc acaacgcgaa acggagaaga cggggtcacg gcgccatctt
     2581 ccctgatctc gcccgatgcc acccgttagg ttgaagccat gggattcacg cagggaaccc
     2641 acagaaacac gaggcggacg gtttggctgg ccgttgcggg attgattctg tcccttctgc
     2701 tggtcgccgc ggtttgtcct tccccggcgg aagccatcca gtacttcggc aagaagcccg
     2761 gcaagtggga agccgagatc gagatccgga acggtttcat ccggtccatg cacctgaagc
     2821 ttccgtcgaa ctgccgcgac caggagcgcc cgccgggcct ggacctggag atcaaccggg
     2881 attcgaagct ccggctccgc cggtacggcg gcttctaccg cgagtcggtg acgcgctgga
     2941 gcaagtcggt cctgaagggc cgggtgatcg gcaacagggt ccagggatcc ctctggtttg
     3001 cggacctcgg tgattacgtc gaatgctgga ccggaaccgg ggagaagccc tcctgggtca
     3061 ggttcgccgc ccgggccgtc cccccgaaat aggccggccg ccccccgggt ctctacgaag
     3121 cgggatcaat cgagaaggca aaagtggcca tatagcgctc acttctgcct tctcgcttac
     3181 ccgcagcagc ctgggaggag gtcgctccga tcccccgggg cggttcagcg ggtggcgcgg
     3241 cggttcagcg ggtggcgggg atgacggcga tctgttcgag gtcgaacttg ccgtcggctt
     3301 cttcgggatc gtcgccgagc gggtagttgc cggtgaagca ggcgtcgcag tggacctcgg
     3361 ccggggtgcc gaccgcttcg tagacgcctt ccatcgagag gtaggcgagc gagtcggcgc
     3421 cgatgtgctc ggcgatttgc tcgatcgtgc gctcgtgggc gaccatctcc tcgcgcgccg
     3481 acatgtcaac cccgtaattg cagccgtagc gaatcggcgg ggcggagatg cggaggtgga
     3541 cctccttggc gccggcgtca cggagcatgc cgacgatctg gcgggtggtg ttgccgcgga
     3601 cgatcgagtc gtcaaccacc accacgcgct ggccggagat gatctccggc agcgggttga
     3661 acttcatgcg caggccgtgc ttgcggagct cctggcccgg ctggatgaat gtgcgggcga
     3721 catagcggtt cttgatcaga ccgtcatcct gcggcaggcc ggaggcccgg gcgtaaccgc
     3781 gcgcggccgg ggtgccggag tcgggcaccg cgatgaccag gtctgcctcg gccggggcct
     3841 cgcgccagag gatctcgccc atccggccgc ggacctcctg caggcgcttg ccttccagct
     3901 tggtgtcggg ccgggagaag tagatgtgct cgaagatgca catcgcggtg cgctccgact
     3961 tgatcacctg gcgcgactcg aggccgcgtt cgctcaggga gatcatctcg ccgggactga
     4021 cctcgcggac cagcttggcg ccgatgatgt cgaaggcaca actctcggag gcgaagacat
     4081 acgagccgtc gggaagctgg ccgagtgaaa gcgggcggat gccgaagggg tcacggaagg
     4141 cgacgatcgc gtgcttggtc atcaccacgg tcgagtaggc accttcgagc tggggcatca
     4201 cgttggcaac cgcatcctcg atgtggcggc cgtagtcgct ggaaatgagg gcggcgatga
     4261 tttccgagtc ggtcgtgccg cggaactcga gtcccttgcg acgcaggcgg ttgtagatct
     4321 cgaccgcgtt ggtcaggttg ccgttgtggg cgagggcgag ttcgcgaccg tcgtcacgcc
     4381 agatcggctg ggcgttttcc catgagccgc cgccggtggt cgagtaccgg acgtggccga
     4441 tcgccatgtc accggtcagg gcccgcagct tgtcctcgtc gaagacctgg gagaccaggc
     4501 cgccgtcccg catggtcgtt atgtgaccgc cttcgcaggt cgcgatcccg gccgattcct
     4561 ggccacggtg ctgaagcgcg tacaggctga agtacgccaa acgggacacg tcgtgtccgg
     4621 gggcatacac gccgaagaca ccacactcgt cgcgtggtcc ctcgcggtcc aggtcgatcc
     4681 gctcagtcac aggtcaggcc accaagaatg gaaaaggaca aaggtgcagg cagtctgcct
     4741 gcattggcag attagcagcg ccccccggtt tgcttgccga ttccccacaa aacagccgca
     4801 aatcgcgggt ttggcagccg tcgagccgtt cgatgtagag tggatgcgtg gccgtgatgc
     4861 ggggacagcg gggactgggt ctgttggtgg tcggagcgat tctcgcctcg gcgctggtcc
     4921 tcttgcccgc aacctccccg gcggcgacca agaaacagaa gcaatgccag cgggcgatcg
     4981 cgaccgtctt caccttcaag aagggcgtgc cgaagaagaa gcgccggaag gccgtgaaga
     5041 tcatctgcca gacgatgacg gtcggcaagc agggaccgaa gggcgcgaag ggtgatcccg
     5101 gggccagggg cgccaccgga gcgaccgggc ctaaaggggc aaccggatcg gcgaacggtg
     5161 tgaccggacc cgtcgggccg accggagcga cgggtcagaa gggtgcgacc ggggcgatcg
     5221 gtttgccggg aatcaccggg gcgaccggcg ccaccggctt tcagggattg atcgggccga
     5281 ccggaccgat cgggctgccc ggactgaccg gggcaaccgg cgcgactggt ttgatcggtg
     5341 agatcgggcc gaccgggccc accggactga ccggtctcat cgggccgacc gggccgaccg
     5401 gcctgacggg cgccgtgccg atcctcatct cggacatccc gtctttctcg gcccaggcga
     5461 tgtccgacgt gatcaacccg atcaccaact ggaacgagga ttacgactcg acgaattcct
     5521 tcaaccctgt cacgggggtg tttaccgccc cgacgaccgg ctcgtacctg atcgaaccgg
     5581 acatcacgac cggaccggcg tcagaggtga ttgccggcgg cggccagcca cccctggttg
     5641 ccgtcaacgt gaacaacgtc gacgtggacg tccaggcgtt ccccctgttc aacgtcgaca
     5701 tcaacctgat tctcgccctg gacgttcccc tccagacagc acaggcatcg ccgaccacct
     5761 tccagcagct cgacgcgggg gatcaggtct cgatccagat catcaagcag aacggcgtgg
     5821 tgtacgacac ctacggcgac ctgatgatca ccaggctgcc gtaacttcag gccccggccg
     5881 gaacgggttc ggcggcgcgg agctccgcgg cgaggctgac cgcgtcatac gcgccgacgt
     5941 gacggacccc gttgacgaag aaggtcgggg tgccgtgggc acccgaggcg tcaccagagg
     6001 cggcgtcctg ctggacccga cgggcgaggc gcccggcctg gacatcctcg acgaagcggt
     6061 cgacgtccat gtccagcttc gcggcgtatc cgatcaggtc ttcgatgtcg aggtcgccct
     6121 gatggtcgta gagctcgaca tgcatttccc agaacttccc ctgctccccg gcggcctcgg
     6181 ctgcctcggc cgcgagttcg gcgtgcgggt gcacgtcaac cagcgccagg tggcggtaaa
     6241 cgtagagcag ctggtcgccc atccgctcgc gaagttcccg gaccatcccg gttgcccggc
     6301 cgcagaatgg acattcgtaa tcgccgtact cgaccagcac aaagggcgcg tcgggactgc
     6361 cgaggaaatg gtcgcgatcc ggatcgaaag gcaggctgag cttgcgaggc aggccggcac
     6421 tgacctcgcc gcggaagacc gccgcgagct tgaagatcgt ccaggccagg gcgacggaga
     6481 tgatcgaggc gaggaagatg ccgaccaccg catgatcctg ggtctgctgg tcatcgaagg
     6541 cgagccgggc gatcagcagg gaaaccgtga agccgatccc ggagagggcc gcgccgccga
     6601 ccgtctggcc tttaccgatc ccgaccggca tctcgccgat cctggtcctg atggccgtca
     6661 ccacaccggt cgtgatgccg agcgtcttgc ccagcacgag accggccacc acgccccagg
     6721 tcagggtcga accgagcgca tcgccgagca ccccgccgcc gaggtccacc ccggcgttgg
     6781 ccagcgcgaa gagcggcacc accacgtaac tcgtgaaggg gtgaaagacg gtctggaacc
     6841 gttcgtttgg cgagagcgcc tgctgaacgc tgaggttggc cgagcgggcc acctcgggca
     6901 ggggcgactg gcggaaggcc cggacccgcc gggcggcctt ctcgaccttc ttcggctggg
     6961 gcgggtaagc ggcgaccagc agcccggcga tcatcccggc cagggtcgcg tgaaagcccg
     7021 attccatggc cgcgatcacc gccaccccga cggtcgcgaa gtagatcccc gatcgccaga
     7081 ccccgatccg cgggtagagc cagatcagaa agagcgcggc gacgcagatc gcgatcgcgg
     7141 tgtagtccag cgcctccgaa tagaagaggc cgatcacggt gatcgcgacg atgtcatcga
     7201 cgatcgccag ggtgagcagg aagacccgca gctgggtcgg gaacttcgga ccgacgatcg
     7261 ccagcgcccc gagcacgaag gcggtgtcgg tggcgatcac catgccccag ccgtgctcgt
     7321 ggccggaacc ggagttgatc gccaggtaga tcagggcggg aaagatcatc ccgccgatac
     7381 cgcccatcac ggcgatcaga acccgcttgc ggtcggtcag ctcgcccatc gccatttccc
     7441 gccggacctc catgccgatc acgaagaaga acatcgccat cagcgcctcg tcgatccagt
     7501 acctcaggtc gtgtgcgatc gagaggtcgc cgaccttgac cgagagttcc gtgctccaga
     7561 agctgaagta cgagtcgctg aaaggtgcgt tcgaccagat cagcgcggcg acgccggcca
     7621 gcagcagcag cccggcgctg ccggattcgg tcgccaggaa ctggcgcatc ggcttcgcga
     7681 cctgggccgc aagatcgcgc ctgctctcgg ttgtatcggg atcgtgaagg ggcttcacca
     7741 ggtccctgtt gaacctaccg ggtcagcatg ttggtctcac cagcggtcgg agcccgttca
     7801 tccgtcattt ctttctgtga accggcgggt tttacttgga ggcggagcgc agctcaccga
     7861 ctacttaatg cgtgatgccc gcctgatctg actcgatgcg ctcctcgagg gattcaaagg
     7921 cggcttcggc ttcgctgacg aggatttcca cgcggcggtc ggaggcggtg acgatcacct
     7981 cgttgccccg gacctcaccg atcacctcga tctcgacgcc gcggccacca agcttctcca
     8041 gcccggcttc ctcgccggcg aggatgaagc ccccggggcc ctcgccgaag agggcatcct
     8101 ccgccgaaca gtcgcgcttg gcgagcaatg cggtcaggtg ggcgtcgcag ccgactccgg
     8161 agccgatcgc caattcggcc agggcggtgg cgataccgcc gtcggaaaca tcggtggcgg
     8221 tgcggatcga accggaacgg accgcttccc ggaccagttc gatcgcctgc cttaccgggg
     8281 cgatctcgaa ctccggcagg cccttgccga gctggccgcg ctgcttctcg agctcggaac
     8341 cggccagcga ggggtcgaac gggccgacca gggcgatcgc atcaccctcg cgccagggct
     8401 caccggccac gcgggacgcg tcgggcagct cgccgaccat gccgaccacc ggggtcgggt
     8461 agatcgggcc ctgcggagtc tcgttgtaga gcgagacgtt gccgccgacc acgggcgcct
     8521 cgagcgcgcg gcaggcctcg gccaggccgt cgatcgagcg accgagctgc caggcgacgg
     8581 tcggcttctc cggattgccg aagttgaggc agttggtgac gccgagcggc tcggccccga
     8641 ccacggcgag gttggccgcg cattcgagcg tggcttcgat cgtgccctga taggggtcgc
     8701 aggcgacccg gcggccgttg ccgtcgatcg cgaccgcgat cgcccggccg gactcgggaa
     8761 tgttgaggac ggcggcgtcg gcggcaccgg cccggtgggc ggtgcgcgaa ccgacgatcg
     8821 agtcgtactg ctcgaaggcc cagcgcttgg aggcgatcga aggcgaggcg agcagggcca
     8881 gcgcgatgtc actggccgag gcatcggcag ggatggtttc gcggttggga taggtccact
     8941 cgcccgggtc ccctgggtcg aggtcataga gcgggcagcc gtcgaccagg tcctcgaccc
     9001 ggatctcacc caccgtttcg ccatctttca ggatgcggaa ggtgccggaa tcggtgacct
     9061 cgccgatcac cgcggaggcg gtctgccact tggtgcagag cgcgagcacc tgctcgacct
     9121 tgcccggctc gaccacggcc agcatccgtt cctgtgattc ggaaaccatg atctcgaagg
     9181 gctccatgtc ggcttcccgg agcggaacca gtccgacgtc gatatcgatc ccggtccctc
     9241 cggcggaagc catctcgccg gccgaggagg tgagcccggc ggcaccgagg tcctgaaggg
     9301 aaacgagcag gtcttcggcc agaagttcct ggcagcactc gagcagcttc gactcctcga
     9361 aggggtcgcc gatctggacg gtcgggcgct ttgcgtcatc gccttcatcg agttcggccg
     9421 aggccagcac cgaggcgccg ccgatgccgt cgcgaccggt cagcgaaccg aagagcacga
     9481 ccttgttgcc ggccccggcc gcggccgccc ggaccatctc gtcggtgcgg gcaaggccga
     9541 cacacatcgc gttgaccagc gggttctgtt cgtagggtgg ctcgaagtag atctcgccgc
     9601 cgaccgtcgg gatgcccatc gaattgccgt agtggccgat cccgcggacc acgccgtcga
     9661 ccaggtaatg ggtccgttcg
//
LOCUS       DAOQKN010000084         9633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_3358_length_9633_cov_5.571100, whole genome shotgun sequence.
ACCESSION   DAOQKN010000084 DAOQKN010000000
VERSION     DAOQKN010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 9633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9633
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3358_length_9633_cov_5.571100"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="PLJ64_07020"
     CDS             <1..705
                     /locus_tag="PLJ64_07020"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNC93267.1"
                     /translation="YLPTSDERFRLALLFSFAAITGGWWLAGPVVGFGLAVAAPMLAG
                     WLISRGRRRYRRSVEKSLPEVSRAIADCLSAGHSPRGALVAATASLEGPVRSEFQTMR
                     YELEAGAPTTDAIRNLATRFDSPRVDAFATALISQRLAGGDLAALLRRFAEGAAERDR
                     VAEDARSATAQARFTGYLVVAMPLGAALFTELLRPGFFASILGSVPALVLVGLSLLLQ
                     LAGFILISRLARIGVT"
     gene            702..1574
                     /locus_tag="PLJ64_07025"
     CDS             702..1574
                     /locus_tag="PLJ64_07025"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNC93268.1"
                     /translation="MSLLPALIGVAAIILGALGLREITREGGGGSGDGPTAAARPAGQ
                     IADGRGIRSGRSGGGDSGEFPKLGDRIRRAGLEAVLAPRSVLLAKGCCAAVGMLFGSL
                     FTGVLPGRLAPVILIAVAAGGFILPDFMLERTASRRHRGMVAALPDVLDLLAVSVQTG
                     RGLGGCLLDLASSGRGPLIAEMSRAGDDMAWGSGQAAALEGLRQRVGGPEITSFVSTL
                     ERSRRLGSPLADQLRRQSATLRQDQRRTIEEQAARAAPKIQLVIALILVPSVLLLIVA
                     ALAANADSLINVGY"
     gene            complement(1568..3238)
                     /locus_tag="PLJ64_07030"
     CDS             complement(1568..3238)
                     /locus_tag="PLJ64_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93269.1"
                     /translation="MNPAKGVRPQAILALASVASVALNMWIGRGITFSTDELVWFMLS
                     PDLDLSGAFHPYVGHLILTSKALYKLIFETIGPDYRVIRLAAAMVLVGVVWILYRLLR
                     RYCGEWPALAISLVVLFFSGDPAHVFHGNGITVLGSVGLGLFALLMLSEPATRRSVLA
                     CLALCLGVATYSQTLPFLVGTTVFLIASKRIRDLWVPAIPFLGYFAWWIWARDLPGSS
                     ESSLEPERIFMLPVWGFRATGAVAESLIHLPDGVASGIQTTIGLLIAAGLAALLVWLL
                     GKGRANPLFFAGAATLLTMWALVIVVPVEDRNFDSSRYMYPFLLASVITFGSLIRPGK
                     PGRTLVWVAGIAVAALCLNGVVEQARHNDQQRAGVTVLLRSGLAGVEAVGPTDPSLTA
                     DDLATQEEDFFLNLPFRAVDDLEIPSLAAYTAAVDQYGRLGFTGSEIGENGPGSALIA
                     DRSAYRVSGIGSPRPLPEGARDLPCRRPAYLLPGPRGVAVPPGLFELRNPSGHPAALR
                     VFRFSHAGTPAGRVPARSAALVGGPQNHGNAGLRMRSDLTLTVCHLAQ"
     gene            complement(3235..4149)
                     /locus_tag="PLJ64_07035"
     CDS             complement(3235..4149)
                     /locus_tag="PLJ64_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93270.1"
                     /translation="MSEEAKPGGTFEGASAEGEVAVTHANQLRSAKFREMTRKKSTWA
                     WIIGLSVLAGLIFAGLGFPAMGGAAFVIVFLGSLLVVFMIADSRAEDAFYDYYCETHG
                     LTRSTSPEIGELTPLLRMGDKSNTDEMFSGELSPGMDGNLVLYTYTEVHRDSDGDETE
                     TDYPFTIIHVEMPEIVQHMTDLQVHRAGFKLLDGIQDKFLGNMQRVTLESEAFAKRFE
                     VFVNDSQDPIWTRRLFSPSFIVWLTESPPDSFAFELENGHLVAYVSRHMDSTESLENV
                     TRVGTYVANRLLQEVAESSPKAARETGS"
     gene            complement(4146..5036)
                     /locus_tag="PLJ64_07040"
     CDS             complement(4146..5036)
                     /locus_tag="PLJ64_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93271.1"
                     /translation="MTEISDANQLRGAKFKELMSKAKTWIIIVVCSVIAAAAGMIVGP
                     AIAGGAFVLVFLIGIVITFMIADSRAATAFYEAYAQARGLTHQSGGILGGSTPLLRKG
                     DRTRVDQQFNGMLSEGIEGTLALYTYTVTSTDSKGNETSTDYPFTVIMVNMPGTVQHL
                     TDLRVQRQSGLKLFEKFEDAFRGDFGRVTLESEAMRDRYEIFVRKEQDPVWVRRLFSP
                     SFIVWLTDSPPKKFAFELENGWLCAYVPKHRDDTEGFDEMIRLGTHVAGRLEEEVGQS
                     SPAEAHHDSPEVKIEVEEEK"
     gene            5249..5686
                     /gene="mraZ"
                     /locus_tag="PLJ64_07045"
     CDS             5249..5686
                     /gene="mraZ"
                     /locus_tag="PLJ64_07045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HNC93272.1"
                     /translation="MAFRGQHEHSLDSKDRLTVPRKFRAALDEGYVLAKGPDACLWLY
                     STKDFDAMEERYVAPHSPFGADARNLRRMINASADEGNLDAAGRIRIPDNLKELAGIE
                     GACTVVGAGDYIEIWNEGTWAKLSQDLLSQFGDVAENLAGSTE"
     gene            5683..6729
                     /gene="rsmH"
                     /locus_tag="PLJ64_07050"
     CDS             5683..6729
                     /gene="rsmH"
                     /locus_tag="PLJ64_07050"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNC93273.1"
                     /translation="MTAAARLAPIGAKVSDSRQSHNPFDRPEQGPRGHLPVLAGELVD
                     LIGPQLGETVIDCTFGAGGHARLVAERIGAEGTLIAIDRDPVAQAHFQAFETEAACRT
                     RFVAAEFSQALKYLQNEGIRASALYMDLGMSSLQVDAAERGFSYSHDAPLDMRMDPRQ
                     ELTAEVIVNTWSEQQLAQMLRELGEERYAGGISRQIVARRPLTSTSDLVEAIKAGMPA
                     AARFGSGHPARRSFQALRIAVNGELEAVDAALPIAWDSLEIGGRMAVISFHSLEDRRI
                     KRFFNDLATGCTCPPELPVCVCGHEPEAEVLTRRPISAGEEEVGMNPRARSARLRGAL
                     KLREANENPAGEVR"
     gene            6729..7214
                     /locus_tag="PLJ64_07055"
     CDS             6729..7214
                     /locus_tag="PLJ64_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93274.1"
                     /translation="MGAPARTIPVLPARVPSRRTVPHRRPATRRRPGSRQHSGLRLIG
                     RSAHAVTHFPESGLVRTAATGRRWIFVIGALLLAIVAINVVTVSYGAMASKLETQIES
                     IERQNAILKSSQTAALAMPRVQAQAEAEGMYLPEPDAIHYRSFQNSDYAAAAERLAAG
                     G"
     gene            7214..8875
                     /locus_tag="PLJ64_07060"
     CDS             7214..8875
                     /locus_tag="PLJ64_07060"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNC93275.1"
                     /translation="MRLQRPERRIGLLFAVSLVLLLGLFARAFLLQVVQGSSLASQAK
                     GQQEDIIEVPGLRGSILDRNGQPLAGSEAGATIYATPYQIKDPPEEARQVAKALDADP
                     GEVLDAITQPGGFSYVDRKVDLKKAEAVDKLGLEGIGQVPDTLRVRPQGDIASQVIGA
                     VSDEGEGLTGIEAGQDGVLKGVNGERRLVKDALGDPISLDNVRDAEDGQPVQLTLDSN
                     IQAEAEKVLAGIGETSNPINASAIVMDAQSSDVLAMANWPPVDLDNLSQAKPEDLLNT
                     GTSYTYEPGSTFKAITVASALETGTVTPSSTFTLAPSIQVFDRVIEESHPRGTVTLDV
                     GDILAQSSNVGAVTIGLEMGATKFSRWIGRFGFGKPTGIDYPGEEQGIVLPYSDWSGS
                     TIGNVPIGQGLAVTPIQMVQAYGALANGGILRPPRLIEKIGGEEVPEQKGHRIIGPEV
                     ASEVRDMLKGVLAPGGTASEVSVPGYSLAGKTGTAQVATKYGYSDTKYVASFVGLAPA
                     DNPRIVVSIMVNQPQGAHTGGEVAAPGFGELAAFALPYLGVPTDQ"
     gene            8984..>9633
                     /locus_tag="PLJ64_07065"
     CDS             8984..>9633
                     /locus_tag="PLJ64_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNC93276.1"
                     /translation="MRLDQLARDLASSDLVGDPGTDISSLQFDNRKVTQGALFFCVRG
                     EKSDGHDFAPAAVEAGAAALVCEKRLDLAVPQLVVGDTRASMAPIAACFNGYPTRDMK
                     LAGITGTNGKTTTAFLLRDILEYAGLPSGLMGTVKQVVGGVEEEVVRTTPEAIDLQAS
                     FRRMLEAGDEACVMEVSSHAMVMHRADSIDFDLAVFTNLTQDHLDFHSDMEDYFQA"
BASE COUNT         1639 a         3115 c         3285 g         1594 t
ORIGIN      
        1 tacctgccga cttccgacga acgcttccgg ctggccctgc tgttcagctt cgcggcgatc
       61 accggcggtt ggtggctggc cggcccggtg gtcggcttcg gcctggcggt tgcggcgccg
      121 atgctggccg gctggctgat ctcccggggg cgccggcgct accggcggtc ggttgagaaa
      181 tccctgccgg aggtctcccg ggcgatcgcg gattgcctca gcgccggtca ttcaccgcgc
      241 ggcgccctgg tcgccgcgac cgcctcgctg gagggtccgg ttcggagcga gttccagacg
      301 atgaggtacg aactcgaggc cggggcgcca accacggacg cgatccgcaa tctcgccacc
      361 cggtttgact cgccgcgggt agatgccttc gccaccgccc tgatcagcca gcgactggcc
      421 ggcggtgacc tggcggcgct ccttcgccgc ttcgccgagg gagccgcgga gcgcgaccgg
      481 gtggcggagg atgcccgatc ggctaccgcc caggcccgct tcaccggcta tctggtggtg
      541 gcgatgccgc tcggagcggc acttttcacc gagttgctca gaccgggctt cttcgcctcg
      601 atcctcggtt cggtgccggc cctggtcctg gtcgggcttt cgctcctcct gcagctcgcg
      661 ggattcatcc tgatctcgag gctcgcgcgg atcggtgtga catgagcctg ctgccggccc
      721 tgatcggggt tgccgcgatc atcctcggag cgctcggact ccgggagatc acgcgggaag
      781 ggggaggagg ttccggtgac ggaccgaccg cggctgcccg gccggccggg cagatcgcgg
      841 acgggcgtgg catccggagc ggccggtccg ggggaggcga ttcgggggaa ttccccaaac
      901 tgggcgaccg catccgccgg gcgggactcg aggcggtact ggcgccgcgg tcggtgctgc
      961 tggccaaggg ttgctgcgcc gcggtcggga tgcttttcgg ttcgcttttc accggcgtgc
     1021 tgccaggcag gctggctccg gtcatcctga tcgcggttgc cgccggcggt ttcatcctgc
     1081 ccgacttcat gctggagcga acggccagcc gccggcaccg gggcatggtc gccgcgttgc
     1141 ccgacgtgct cgacctgctg gcggtctcgg tccagaccgg tcgcggtctc ggcggttgcc
     1201 tgctcgacct ggccagctcc ggccgcgggc ccctgatcgc cgagatgtcc cgggccgggg
     1261 acgacatggc gtggggtagc ggccaggcgg cggcactgga aggtctcagg cagcgggtcg
     1321 gcggtcccga gatcacctcg tttgtgtcga ccctggaacg ctcgcgccgg ctcggctcgc
     1381 cgctggccga ccagctgcga aggcagtcgg cgaccctcag gcaggatcag cggcggacga
     1441 tcgaggaaca ggccgcccgg gccgcgccga agatccagct ggtgatcgcc ctgattctgg
     1501 tcccgtcggt cctgcttctg atcgtcgctg cgctcgccgc caacgccgac agcctgatca
     1561 acgttggcta ctgagccagg tggcagacgg tcagcgtcag gtcgcttctc atccgcaacc
     1621 cggcgttgcc atggttctgc gggccgccaa ccagagctgc cgaacgggcc ggtacccggc
     1681 cggccggggt gccggcgtgg ctgaaacgga agacgcggag agcggccggg tggccggaag
     1741 ggttgcggag ttcaaaaagg ccaggcggga ccgcgacccc tcgtggtccg gggagaaggt
     1801 aggccgggcg tcggcaaggc aggtccctgg caccttcggg cagggggcgc ggagatccga
     1861 tgccggagac ccggtaagcg gaccggtcgg cgatcagggc ggagccgggg ccgttctcgc
     1921 cgatctctga tccggtgaaa cccagccggc cgtactgatc gacggctgcg gtgtaggcgg
     1981 ccagcgaggg gatctccaga tcgtcgaccg cccggaaggg gaggttgagg aagaagtcct
     2041 cttcctgggt ggcgaggtca tcggcggtca gcgaggggtc ggtggggccg accgcttcca
     2101 cccccgccag tccggagcga agcaggacgg tcaccccggc acgctgctgg tcattgtggc
     2161 gagcctgttc gaccacgccg ttgaggcaga gcgcggcaac cgcgattccg gccacccaga
     2221 ccagggtgcg gcccggcttc ccgggcctga tcaggctgcc gaaggtgatc acgcttgcca
     2281 gcaggaacgg atacatgtag cgggagctgt cgaagttgcg atcctcgacc ggaaccacga
     2341 tgaccagcgc ccacatggtc agaagcgtcg ccgcccccgc gaagaacagg ggattggccc
     2401 ggcccttccc gagcagccag accagcagcg cggccagacc ggcggcgatc agcaggccga
     2461 tcgtcgtctg tattccggag gcgaccccgt ctggcaggtg aatcaggctc tcggctacgg
     2521 cgccggtggc ccggaagccc cagaccggca gcatgaagat ccgttccggt tccagtgagc
     2581 tctccgagga accgggcaag tcccgggccc agatccacca ggcgaaatat ccgaggaacg
     2641 ggatcgccgg aacccaaagg tcccggatcc gtttcgaggc aatcaggaaa acggtggttc
     2701 cgaccaggaa gggcagcgtc tgcgaatagg tggcgacacc tagacaaagg gcgaggcagg
     2761 ccagcaccga ccgccgggtc gccggttcgc tcagcatcag cagggcgaag aggcccaagc
     2821 cgacagaacc gagcacggtg attccgttgc cgtggaagac atgggcaggg tcgccgctga
     2881 aaaaaaggac gaccaggctg atcgcgaggg ccggccactc gccgcagtag cggcggagca
     2941 gccggtaaag aatccagacg accccgacca gaaccatggc cgcggccagg cggatcaccc
     3001 ggtagtccgg cccgatcgtc tcgaagatca gtttgtagag ggctttcgag gtgaggatca
     3061 ggtggccgac gtaggggtgg aaggcgccgg aaaggtcgag atcgggactg agcatgaacc
     3121 agaccagctc gtcggtgctg aaggtgatcc cccggccgat ccacatgttg agggcgacgc
     3181 tggccaccga ggccagcgcc aggatggcct ggggccggac cccctttgcc gggttcacga
     3241 cccggtttcg cgcgcggcct tcggcgagga ctcggcgact tcctggagca accggttggc
     3301 cacgtaggtg ccgacccggg tcacgttctc caagctttcc gtgctgtcca tgtgcctcga
     3361 gacgtaggcg accagatggc cgttctcgag ttcgaaggcg aaactgtcgg gcgggctttc
     3421 ggtcagccag acgatgaagc tgggggagaa gaggcgccgg gtccagatcg ggtcctggct
     3481 gtcgttgacg aacacctcga accgcttggc aaaggcttcg ctttcgaggg tcacccgctg
     3541 catgttgccg aggaacttgt cctggatccc gtcgaggagc ttgaagcccg cccggtggac
     3601 ctgaaggtcg gtcatgtgct ggacgatctc cggcatctcg acgtggatga tcgtgaaggg
     3661 gtagtcggtt tccgtttcgt caccgtccga atcgcgatgg acctcggtgt aggtatagag
     3721 cacgaggttg ccgtccatcc cgggcgaaag ctcgccggag aacatctcgt ccgtgtttga
     3781 cttgtcaccc atgcggagca gcggggtcag ttcgccgatc tcgggcgagg tgctccgggt
     3841 caggccgtgc gtttcgcagt agtaatcgta gaaggcgtct tcggcccggc tgtcggcgat
     3901 catgaagacc accagcaggg agccgaggaa gacgatgacg aaggcggcgc cccccatcgc
     3961 cgggaagccg agcccggcga agatcagtcc ggcgaggacc gaaagcccga tgatccaggc
     4021 ccaggtcgac ttcttccggg tcatctccct gaacttggcc gagcggagct ggttggcgtg
     4081 ggtgaccgcc acctcgcctt cggcgctcgc accttcaaag gtgccgccag gcttcgcttc
     4141 ctcgctcact tctcttcttc cacttcgatc ttgacttcgg gcgagtcgtg atgcgcttcg
     4201 gcaggtgagc tctggccgac ttcctcttcg aggcggcccg ccacatgggt accgagcctg
     4261 atcatctcgt cgaagccttc ggtgtcatcg cgatgcttgg gcacataggc gcagagccag
     4321 ccgttctcca gttcgaaggc gaacttcttc ggcgggctgt cggtcagcca gacgatgaag
     4381 ctgggcgaga acagacgccg gacccagacc ggatcctgtt ccttcctgac gaagatctcg
     4441 tagcggtccc gcatcgcctc gctttccagg gtgacccggc cgaagtcgcc gcggaaggcg
     4501 tcctcgaact tctcgaacag cttcagaccc gattgcctct gcaccctgag gtcggtgagg
     4561 tgctgaacgg tgcccggcat gttgaccatg atcacggtga aggggtagtc ggtcgatgtc
     4621 tcgttgccct tcgagtcggt gctggtaacc gtgtaggtgt agagggcgag ggttccctcg
     4681 atcccctcgg agagcatgcc gttgaactgc tggtcgaccc gggtccggtc acctttccgc
     4741 agaagcgggg tggagccgcc gaggatgccg ccggactgat gggtcagtcc gcgtgcctgg
     4801 gcgtaggcct cgtagaacgc ggtggcagcc cggctgtcgg cgatcatgaa agtgatcacg
     4861 atcccgatca ggaagacgag aacgaaggcg ccgccggcga tcgccgggcc gacgatcatc
     4921 ccggcggcag cggcgatcac gctgcagacg acgatgatga tccaggtctt cgccttgctc
     4981 atcaactcct tgaacttggc gccgcgcagc tgattcgcat cggagatctc ggtcatatcg
     5041 ggaggttatt tcatgaccgg gcggtgcaat cgccgttgca acttttggaa gaaagtgggg
     5101 ggttgtgttg caaagtgggg caaagtgggt tacattgcca tcgagttgaa gccaagggcc
     5161 ggggtgatgg agctcctccc gctctcacca ccccggccca actctttccc ggtcaaccca
     5221 cggcgaggca aagaaaggac gaggagcggt ggcgttcaga ggccagcacg aacacagcct
     5281 cgattccaag gaccgcctca cggtgccccg caagttccgt gccgctctcg acgagggata
     5341 cgttctcgcc aaaggcccgg atgcctgcct ctggctctac tcgaccaaag acttcgacgc
     5401 gatggaagag cgctacgtcg ctccccacag cccgttcggg gccgacgcgc gcaaccttcg
     5461 ccggatgatc aacgccagcg cagacgaggg caatctcgat gcggccggcc gcatccgcat
     5521 tccggacaac ctcaaggagc tggccggaat cgagggcgcc tgcaccgtgg tcggcgccgg
     5581 tgactacatc gagatctgga acgaagggac ctgggccaaa ctgagccagg acctgctcag
     5641 ccagttcggc gacgtggccg agaacctcgc cgggagcacg gagtgacagc cgccgcgcgg
     5701 ctcgccccga tcggagccaa ggtgagcgac tcgcgccaga gccacaaccc tttcgaccgg
     5761 cccgaacagg ggccgagggg gcacctgccg gtactggccg gggagctggt cgacctgatc
     5821 ggcccgcagc tcggcgagac ggtgatcgac tgcacattcg gggccggcgg tcacgcccgc
     5881 ctggtcgccg agcggatcgg agccgaaggt accctgatcg cgatcgaccg tgacccggtc
     5941 gcccaggctc acttccaggc gttcgagacc gaggccgcct gccggacgag attcgtcgcc
     6001 gccgagttca gccaggctct gaaatacctg caaaatgaag gtattcgcgc ttcggcgctg
     6061 tacatggatc ttgggatgtc ttcacttcag gtggacgccg ccgagcgtgg cttctcctac
     6121 tcacatgacg cgcccctcga catgcggatg gatccgcgac aggaattgac cgccgaagtg
     6181 atcgtcaaca cttggtccga gcagcagctc gcccagatgc tgcgtgaact cggcgaggag
     6241 cgatacgccg gcgggatcag ccgccagatc gtcgcccggc ggccgctgac cagcacttcg
     6301 gacctggttg aggcgatcaa ggccgggatg ccggccgcgg cccgtttcgg ttccggccat
     6361 ccggcccgcc gcagcttcca ggcgcttcgg atcgcggtca acggtgaact cgaagcagtc
     6421 gatgcggccc tgccgatcgc ctgggacagc ctcgagattg gcggtcgcat ggccgtcatc
     6481 tcgttccact cgctcgaaga ccgccggatc aagcgcttct tcaacgatct cgcgaccggc
     6541 tgcacctgcc cgccggagct gccggtctgc gtctgcgggc atgagccgga ggccgaggtg
     6601 ctcacccgcc ggccgatttc cgccggggag gaagaagtcg ggatgaaccc gagggcccgc
     6661 tcggcccgcc tgaggggggc cctgaagctg cgtgaagcga atgagaatcc cgccggggag
     6721 gttcgctgat gggcgccccg gctcgcacca tcccggtcct tcccgctcgc gttccctccc
     6781 ggcgcacggt ccctcaccgt cggcccgcca cccggcgccg gcccggcagc cggcagcact
     6841 ccggcctgcg cctgattggc aggagcgccc atgcggtcac tcatttcccc gagtcggggc
     6901 tggttcgcac cgccgccacc ggccgacgct ggatcttcgt gatcggcgcg ctgctactgg
     6961 cgatcgtcgc gatcaacgtg gtcaccgtca gctacggcgc gatggccagc aagctcgaaa
     7021 cccagatcga atcgatcgag cggcaaaacg ccatcctgaa gtcgtcgcag acggctgctc
     7081 tggcgatgcc ccgcgttcag gcccaggctg aagccgaagg catgtacctg ccagagcccg
     7141 acgcgattca ctaccgctcc ttccagaact ccgactatgc ggcggccgcc gagcgactgg
     7201 cggccggggg ctgatgcgac tgcaacggcc cgaacggcgg atcggcctgc tcttcgcggt
     7261 cagtctggtt cttctgctcg ggctcttcgc ccgggctttt ctgcttcagg tggttcaggg
     7321 ttcctcgctc gcatcccagg cgaaaggcca gcaggaagac atcatcgagg tgcccggtct
     7381 gcgcggctcg atcctcgacc gcaacggcca gccgctggcc gggtccgaag ccggcgccac
     7441 gatttacgcc accccgtacc agatcaagga tccgccggag gaggccaggc aggtcgcgaa
     7501 ggcgctcgac gccgatccgg gcgaggtgct tgatgcgatc acccagcccg gcgggttctc
     7561 atacgtcgat cgcaaggtgg acctgaaaaa ggccgaagcg gtcgacaagc tgggcctcga
     7621 agggatcgga caggttccgg acaccctgcg ggtccggccc cagggtgaca tcgcctccca
     7681 ggtgatcggg gcggtcagcg acgaaggcga gggactgacc ggaatcgaag ccggacagga
     7741 cggcgtgctc aagggcgtaa acggggagcg gcggctggtc aaggacgcac tcggcgaccc
     7801 gatcagcctc gacaacgtcc gcgacgccga ggacggccag ccggttcagc tgacgctgga
     7861 ctcgaacatc caggccgagg ccgagaaggt gctggccggg atcggggaga ccagcaaccc
     7921 gatcaatgcc tcggcgatcg ttatggacgc gcagagttcc gacgtgctgg cgatggccaa
     7981 ctggcccccg gttgacctcg acaatctcag tcaggcgaaa cccgaagacc tgctcaacac
     8041 cggcacctcg tacacctacg agcccggctc gactttcaag gcgatcacgg tcgcctcggc
     8101 gcttgagacc ggaactgtca ccccgtccag cactttcaca cttgccccct cgatccaggt
     8161 gtttgaccgg gtgatcgaag agagccatcc gcgaggaacg gtgaccctcg atgtcggtga
     8221 catcctcgcc cagtcctcga acgtgggggc ggtgacgatc gggctcgaga tgggggcgac
     8281 gaagttcagc cgctggatcg gccgctttgg tttcggcaag ccgaccggga tcgactaccc
     8341 cggcgaggaa caggggattg tccttcctta cagcgactgg tccggctcga cgatcggcaa
     8401 cgtgccgatc ggccagggcc tggcggtcac cccgatccag atggtccagg cctacggggc
     8461 tctcgccaac ggcggcattc tccggccgcc gcggctgatc gaaaagattg gtggcgagga
     8521 agttccggaa cagaagggcc accggatcat cgggcccgag gtcgcttccg aggtgaggga
     8581 catgctgaag ggggtgctcg ctccgggcgg caccgcctcc gaagtatcgg tgccgggcta
     8641 cagtctcgcg ggcaagaccg gtaccgccca ggtcgcgacc aaatacggct actcggacac
     8701 gaagtatgtg gcctcgttcg tcggcctcgc gccggccgac aatccgcgca tagtggtgtc
     8761 gatcatggtc aaccagccgc agggcgccca caccggcggc gaggttgcgg cccccggttt
     8821 cggtgaactg gccgcattcg cgctgccata tctcggcgtt ccgaccgatc agtgaaattc
     8881 ccggcggagc ctcttccttc cgtccgtgaa tgtgagagga aggggcgggt ttcttttgga
     8941 gggcgggtcg gaacccgcct gaccaggttc taaactcaac gccatgcggc tggaccaact
     9001 cgcccgggat ctggcctcgt ccgacctcgt cggcgacccg ggcaccgaca tatcctcgct
     9061 ccaattcgac aatcggaaag tgactcaggg agcgcttttc ttctgtgttc gcggcgaaaa
     9121 gagcgatgga cacgatttcg cgccggccgc ggttgaagcg ggcgccgcag cgctggtctg
     9181 cgaaaaaagg ctcgatttgg cggtgccgca acttgtggtc ggcgacaccc gcgcttcaat
     9241 ggcaccgatc gcggcctgtt tcaacggcta cccgacccgg gatatgaaac tggccgggat
     9301 caccgggacc aacggcaaga ccacgaccgc tttcctgctg cgggacatcc tcgaatatgc
     9361 cggtctgccc agcggcctga tgggcacggt taagcaggtg gtcggcgggg tcgaggaaga
     9421 ggtggtccgg acgacgcccg aggcgatcga cctccaggcc tctttccggc gcatgctcga
     9481 ggccggggac gaagcatgcg tgatggaggt ttcctctcac gcgatggtca tgcaccgggc
     9541 tgactcgatc gatttcgacc ttgcggtctt caccaacctg acccaggatc acctcgattt
     9601 ccacagcgac atggaggatt acttccaggc caa
//
LOCUS       DAOQKN010000085         9448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_3450_length_9448_cov_5.966890, whole genome shotgun sequence.
ACCESSION   DAOQKN010000085 DAOQKN010000000
VERSION     DAOQKN010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 9448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9448
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3450_length_9448_cov_5.966890"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="PLJ64_07070"
     CDS             complement(<1..869)
                     /locus_tag="PLJ64_07070"
                     /inference="COORDINATES: protein motif:HMM:NF021214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HNC93277.1"
                     /translation="MNLLPTPPLAGISPTVVAMLELIPFTIAVVIYWHRAMTLSWDGR
                     PVPLWRQISFGFGLFLAASMLFNPWGYLAEELVIAHMVEHLLIGDIASLFIVLGLTRS
                     LLQPVLAIPFFNRLQVLTSPVVAFPLWAINMFFWHIPAMYDGAYGGAALHGVEHASFL
                     LFGCLMWMPVFGPLPTPKWFGPGWKVVYVIIVRFTAAILGNVLMWTQVTLYHNYAAGQ
                     AKWGLTAVQDQSTAGVIMMVEGTFLILGVLAWTFFEAAKQSMQKQELIDLAFDRGVDI
                     DEERIERAVKAGHG"
     gene            907..2808
                     /locus_tag="PLJ64_07075"
     CDS             907..2808
                     /locus_tag="PLJ64_07075"
                     /inference="COORDINATES: protein motif:HMM:NF012926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HNC93278.1"
                     /translation="MTPGAGHGHFPRIGSYGFLSDCHTCALVAPDGSVEWFCAPRFDS
                     PSLFGSILDRSAGHFRFGPWDENAPLSRRYEPGTLVMETTWCTETGWVVVRDALSISS
                     WAEQGGPRELMTGHETDRCLVRLVKCIDGEVDMEMVCRPRFKYGTEAAVFSDQGLGQA
                     IAEGAGERLAIATDLNLTLEQGQATGRLKMKHGDSAFVCLSWGEGIGPHPRSYPEATE
                     RIESSRDLWRDWLAQGTFPDHPWRIHLQRSALVLKGLTYTPSGAMLAAATTSLPETPG
                     GERNWDYRYSWIRDSTFALWSMHSLGFEQEARDFMRFVRDRAMESGGDLQIMYGIGGE
                     HDLTEHTLDHLSGYGGAKPVRTGNGAFDQRQNDVWGALLDSIYVHRKALGPGAINDET
                     RDLVRRLVENALEAWPKPDQGIWEARGEPRHYVSSKLMIWVAVERASRLAAEFGVKEN
                     ADRLRAKAAEIKEEIIEKGCRDGVFRQHYETDALDASVLLMPLVRFLPPDDPRIRDTV
                     LAIRDGLDDRGLILRYITDETDDGLHGEEGTFLICSFWMVAALSEIGEKSAAKKLCER
                     LLAASGDLDLYAEELEASSNRHLGNFPQAFTHLALINAVSHVIADERRVDSAPGTPTA
                     VFTEIREMD"
     gene            2853..3548
                     /locus_tag="PLJ64_07080"
     CDS             2853..3548
                     /locus_tag="PLJ64_07080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNC93279.1"
                     /translation="MRSNVARVRERMAAACERAGREPDSVRLLLATKTVEPARIRVAI
                     EAGETLVAENRVQEVRPKFEALEDLRYERHFIGHLQSNKINALVPYISCLETLDRLSL
                     ARKLQNRLAGEGETLEVLVQVNTSGEPTKAGLDPDEVEPFIRTIAGLDALKVRGLMTI
                     GLNAEPEVARPSLASLREIAGRIREENIEGVAMEELSMGMTGDLEVAIEEGSTIVRVG
                     SAIFGSRPPLQSP"
     gene            complement(3643..3777)
                     /locus_tag="PLJ64_07085"
     CDS             complement(3643..3777)
                     /locus_tag="PLJ64_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93280.1"
                     /translation="MLIVIGIVLALLVNWALGIICIVIGLVLLIWPAVTGRGSSGSRV
                     "
     gene            complement(3849..4370)
                     /locus_tag="PLJ64_07090"
     CDS             complement(3849..4370)
                     /locus_tag="PLJ64_07090"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC93281.1"
                     /translation="MVGANEAVTENLLCLRPGAATAGDYEGVFAEPAVQRWLRPPPMP
                     PFAAADFERMARRDRAHWDRHGYGPWAVREREGGAFVGRGGLAWATVAGERQVELPWA
                     VSPALQNRGYATEMALAAIETAREAGLHRVVSLALPDNLASRRVMEKAGLQYVAEIEH
                     VGLPHLLFELDLG"
     gene            complement(4378..4836)
                     /locus_tag="PLJ64_07095"
     CDS             complement(4378..4836)
                     /locus_tag="PLJ64_07095"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNC93282.1"
                     /translation="MSQNDEVIVRFYEAFAAGDADTMAGCYHERVHFHDPVFEDLNGP
                     EVMKMWRMLLGRSDDLEITLGEHQAEGDQGSGHWLAVYTFSQTGRVVHNEIDATFRFE
                     DGLIIDHRDSFDFWKWTRMALGVPGTLLGWSPIVQGKVRKQSKLLLAGAG"
     gene            complement(4871..5932)
                     /locus_tag="PLJ64_07100"
     CDS             complement(4871..5932)
                     /locus_tag="PLJ64_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNC93283.1"
                     /translation="MSTPLALMISLLLLAGNAFFVGAEFALISARRSVIELRADEGGW
                     AARITLHAMERVALMMAGAQLGITLCTLGLGALAEPAIAHALEHPFEGLGIPGSLVHP
                     AALVVALFLVGGFHVVLGEMVPKNIALAGPDRSALALGPPIAAVVKVLHPIIWVLNKT
                     ANGVLRLLGVTPSDEVTSAFTRDEVAGLVEESRREGMLDADETRLLTGALTFEERDAS
                     VVLLPIETMVTLPITVTPAEVEEAAGRTGFSRFPVTRDDEMIGYLHLKDALEVKEKHR
                     NRPIAESWVRPLPTVRLTDQLREVLEVMQSSGAHLARVVVRGARGRGKTVGVVALEDV
                     LEELIGEVRDAAQQIESES"
     gene            complement(5929..7284)
                     /locus_tag="PLJ64_07105"
     CDS             complement(5929..7284)
                     /locus_tag="PLJ64_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNC93284.1"
                     /translation="MIELLLVLVSVALVVACGAFVAAEFSFVTVDRSRVDREADEGDR
                     RARGVRQGLRTLSTQLSAAQVGITVTNLLIGYLAQPSVASLIDAPLESLGVSAGAVDG
                     VALTVAFILANAVTMIFGELVPKNLAIANAMGTAKAVQGFQRGFTRIIHPLVRVTNGI
                     ANWILRRVGIEPQEELASARSPEELTSLVRRSAEKGTLEEPTAELLEKSLEFGELRAD
                     DVMTPRVKVKTLAPDQPVIAVIEAARAGGFSRFPVISEGSEIVEGIVHVKHAVAVPHE
                     DRDDVPIRDVMADPVLVPSTIELDPLLAELRKVGLQVAIVVDEFGSFDGIVTLEDLIE
                     EIVGEVRDEHDRDEDPVREIAQGIWAVSGLMRPDEVEDQIGVRLPEDEDYETVAGLIG
                     FELGRVPGRGDSVRVEATNEEKEPLLADLYVLRMDGLRVDRVRLVVSKPQPEEDGEGE
                     K"
     gene            7469..8941
                     /locus_tag="PLJ64_07110"
     CDS             7469..8941
                     /locus_tag="PLJ64_07110"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNC93285.1"
                     /translation="MASLLRRLLFAVLAATFLISSGASAAAPEPVVDGNQIVDSATGD
                     QFVPHGVNFPGYEYACKQGWNYGEEGTRPAEIASTVAGIASWNINTVRLPLNQDCWLG
                     DDGLPAGPGRTVAGYRQSVEDFVNALNAAGIVAIVDIHWSGPDGVVADGLRPMPDNRS
                     PAFWSSVATTFKDNPSVIFDLFNEPHSRWNADDTKVFGLSWDCWANGGCMAPAERDLA
                     KVSGLSWYRTVGMKKLTEVVRATGATQPILLSGTDYANDLRGWLANAPEDDQLIAGFH
                     NYLEQRCRTETCWDREVAPVAEHVPVLAAEFGQNDCGPPGHMNRFMDWADDHLIGYLA
                     WAWWSLPDEGCHNFALVSNLDGTPLAPAGTALNSHLASLPRVLPQPVKRVSPGLVIKR
                     AQWKRPTLRLRVAIKPNASAAITVQIRLARDSHARRAPRTNRRLITKKLRTGFGFVDL
                     HTAIPTGLKPVLVTVSYPGDKRLLPKTVKRKPGSVSKLTR"
     gene            8969..>9448
                     /locus_tag="PLJ64_07115"
     CDS             8969..>9448
                     /locus_tag="PLJ64_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015575233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNC93286.1"
                     /translation="MTVRQTIEIHGQPVTYHKMGEGPPVLLVHGITSSSRTWKSVMPR
                     LAETHTVIAPDLLGHGRSAKPQGDYSLGAYASGMRDLLVALDVEKVNVVGHSLGGGVA
                     MQFAYQFPDRISRLVLVDTGGIGREVNPALRAAALPGAEFVLPLLFTPTLHDAGLKVR
                     "
BASE COUNT         1666 a         3218 c         3052 g         1512 t
ORIGIN      
        1 ccgtggccgg ccttgaccgc cctttcgatc cgctcctcgt cgatgtccac cccgcggtcg
       61 aaggcgaggt cgatcagctc ctgcttctgc atcgactgct ttgccgcctc gaagaaggtc
      121 caggcgagga ccccgaggat gaggaaggtc ccttcgacca tcatgatcac gccggccgtg
      181 ctctggtcct ggaccgcggt gaggccccat ttggcctgac cggcggcgta gttgtggtag
      241 agcgtgacct gggtccacat cagcacgttg ccgaggatcg ccgcggtgaa cctgacgatg
      301 atcacgtaga cgaccttcca gccggggccg aaccacttcg gggtcggcag gggcccgaag
      361 acgggcatcc acatcaggca tccgaagagc agaaacgagg catgttcgac accgtggagc
      421 gccgctccgc cgtaggcccc gtcatacatc gccgggatgt gccagaagaa catgttgatc
      481 gcccagagcg gaaaggcgac caccggactg gtcaggacct gcagccggtt gaagaagggg
      541 atcgcaagca ccggctggag cagggaacgg gtcaggccga gcacgatgaa gaggctggcg
      601 atgtcaccga tcagcaggtg ctcgaccatg tgggcgatga ccagttcctc ggccaggtag
      661 ccccaggggt tgaacagcat cgaggcggcg aggaagagac cgaagccgaa ggagatctgg
      721 cgccaaagcg ggaccggtcg cccgtcccag gaaagggtca tcgcccggtg ccagtaaatg
      781 accaccgcga tcgtgaacgg gatcagctcc agcattgcca cgaccgtcgg gctgatcccg
      841 gcaaggggag gtgtcggcaa cagattcacg gcctttcagc gtaccggcgc tggtaatttc
      901 accacgatga ctcccggtgc cggccacggt cactttccga gaattggaag ctatggcttt
      961 ctctccgatt gccacacctg cgccctcgtg gcgccggacg gctcggtcga atggttctgc
     1021 gccccgcgtt ttgactcgcc cagcctcttc ggatcgatcc tcgaccggtc cgccggccat
     1081 ttccggttcg gaccgtggga cgaaaatgct ccgctcagcc ggcgctacga gccgggcacc
     1141 ctggtcatgg agaccacctg gtgcaccgag accggctggg tcgtggtccg cgacgcactc
     1201 tcgatctcgt cctgggccga gcagggcggg ccacgggagc tgatgaccgg ccacgagacc
     1261 gaccgctgcc tggttcggct ggtcaagtgc atcgacggtg aagtcgacat ggagatggtc
     1321 tgccgaccgc ggttcaaata cggcaccgag gccgccgtct tctccgacca ggggctcggt
     1381 caggcgatcg ccgaaggcgc cggcgagcgg cttgcgatcg ccaccgacct caacctgacc
     1441 ctggagcagg ggcaggcgac cggacggctg aagatgaagc atggcgacag cgccttcgtc
     1501 tgcctcagct ggggcgaggg catcggcccc catccgcgca gctacccgga agccacggaa
     1561 aggatcgagt cctcacgcga cctctggcgg gactggcttg cccagggcac ctttcccgac
     1621 catccctggc ggatccacct ccagcgttct gccctggttc tgaagggact cacctacacc
     1681 ccgtccggcg cgatgctggc ggcggccacc acttcactgc cggaaacccc cggcggcgaa
     1741 cggaactggg actaccgcta ctcctggatc cgtgattcca ccttcgcgct ctggtcgatg
     1801 cactcgctgg gattcgaaca ggaagcccgg gacttcatgc gattcgtgcg cgaccgggcg
     1861 atggagtccg gaggcgacct gcagatcatg tacgggatcg gcggcgagca cgacctgacc
     1921 gagcacacgc tcgaccatct gtccggatac ggcggtgcga agccggtccg gaccggaaat
     1981 ggcgccttcg accagcgtca gaacgatgtc tggggcgccc tgctcgattc gatctacgtt
     2041 catcgcaagg cgctcgggcc cggagcgatc aatgacgaga cccgggacct ggtcaggcgg
     2101 ctggtcgaga acgcgctcga ggcctggcca aaaccggatc agggcatctg ggaagcccgc
     2161 ggcgaaccca ggcactacgt ctcgtcgaaa ctgatgatct gggtggcagt ggagcgggcc
     2221 tcgcgccttg ccgccgagtt cggggtcaag gagaacgccg accggctgcg cgcgaaggcg
     2281 gccgaaatca aggaagagat catcgaaaag ggctgcaggg acggcgtctt ccgccagcat
     2341 tacgagaccg acgcgctcga cgcctcggtc ctgctgatgc ccctggtccg cttcctgccc
     2401 ccggatgacc cgcggatccg cgacacggtc ctggcgatcc gggacgggct cgacgatcgg
     2461 ggcctgatcc tccgctacat caccgatgaa accgacgacg ggctccacgg cgaagaagga
     2521 accttcctga tctgctcctt ctggatggtc gcggcgctct ccgagatcgg cgagaagtcc
     2581 gccgcgaaga agctctgcga acggttgttg gccgcctccg gcgatctcga cctctacgcc
     2641 gaggagctgg aggcttccag caaccgccac ctcggaaact ttccccaggc tttcacgcat
     2701 ctggccctga tcaacgccgt gtcccacgtg atcgccgacg aacggcgtgt cgattcggct
     2761 ccgggtaccc cgaccgccgt cttcaccgag atccgggaga tggactgatc gaactcgacc
     2821 cgggaagcgt cgatccggct gatgccgagc tgatccgctc gaacgtcgcc cgggtccgcg
     2881 agaggatggc ggccgcctgc gagcgggccg ggcgcgagcc ggattcggtg cgcctgctgc
     2941 tggccacgaa gacggtcgag ccggcacgga tccgcgtggc gatcgaagcc ggcgagaccc
     3001 tggtcgccga gaaccgggtc caggaggtcc gacccaagtt cgaagcactt gaagaccttc
     3061 ggtacgagcg ccacttcatc ggccacctcc agtccaacaa gatcaacgcg ctggtccctt
     3121 acatcagctg cctggagacc ctggaccggc tctcgctggc ccgcaaactc cagaaccggc
     3181 tcgcggggga aggcgagacc ctggaggtgc tggtccaggt caacacctcg ggcgagccga
     3241 ccaaggccgg gctggatccg gatgaggtcg aaccgttcat tcgtacgatc gccggactcg
     3301 acgcgctgaa ggtccgcggt ttgatgacga tcggcctcaa tgccgagccg gaggtggccc
     3361 gcccttcgct ggccagcctg cgggagatcg ccggccggat ccgcgaagag aacatcgaag
     3421 gcgtggcgat ggaagaactc tcgatgggca tgaccggcga cctcgaagtg gcgatcgagg
     3481 agggctcgac catcgtccgg gtgggcagcg caatcttcgg ctcccgcccc ccgcttcaat
     3541 caccctgagg ttcagcgacc gacgaatgcg aagggccccg gcttcccggg gcccttcggt
     3601 caaaatcggc tgtgcctgat cggccgggcg ctacccggcg agtcagaccc ttgagccact
     3661 gcttcctctt cctgtgaccg cgggccagat caacaggacc agcccgatca cgatgcagat
     3721 gatgcccagc gcccagttga ccagcagggc caggacaatt ccgatgacta tgagcaacag
     3781 tgctagtggc atgcctggga ggtacccgga acggcgcccg gcaaacccgc gggccactcg
     3841 aatgcgggct acccgaggtc cagctcgaag agcaggtgcg gcaggccgac gtgctcgatc
     3901 tcggcgacgt actggagccc ggccttttcc ataactcttc gcgaggcaag gttgtccggc
     3961 agggccaggg agacgacccg gtggagaccg gcttcgcgag ccgtttcgat tgccgccagg
     4021 gccatctccg ttgcgtagcc gcggttctgc agcgcgggac tcaccgccca gggcagctcg
     4081 acctgacgct cgccggccac ggtcgcccag gccagtccgc cacgaccgac gaaggcgcct
     4141 ccctcccgtt cccgcaccgc ccagggcccg taaccgtgcc tgtcccagtg agcccggtcc
     4201 cggcgggcca tgcgttcgaa atccgccgcc gcgaacggag gcatcggggg cggcctgagc
     4261 catcgctgga ccgccggctc ggcgaagaca ccttcgtaat ccccggcggt ggctgctccc
     4321 ggccgcaggc agagcaggtt ctcggttacc gcttcgttgg ccccgaccaa accagcctca
     4381 gccggcgccg gcgaggagca gcttcgactg cttgcggacc ttgccctgga cgatcggact
     4441 ccagcccagc aacgttccgg gcacaccgag tgccatccgg gtccacttcc agaagtcgaa
     4501 gctgtcccga tggtcgatga tcaggccatc ctcgaaccgg aaggtggcgt cgatctcgtt
     4561 gtggaccacc cttccggtct ggctgaaggt gtagacggcc aaccagtgtc cggaaccctg
     4621 gtcaccttct gcctgatgct cgccgagcgt gatctccagg tcatccgagc gacccagcag
     4681 catccgccac atcttcatca cttcgggccc gttcagatcc tcaaagacgg ggtcgtgaaa
     4741 gtggactcgc tcgtgatagc agccggccat cgtgtccgcg tcaccagcgg cgaaggcttc
     4801 atagaagcgg acgatcacct cgtcgttctg gctcatgcgc cgatcctatc cagcccggga
     4861 ggtgccgcgg tcaggattcg gactcgatct gctgtgccgc gtcccggacc tcgccgatca
     4921 gctcttcgag cacgtcctcg agggcgacca caccgaccgt cttgccgcgg ccacgggcgc
     4981 cccgcaccac gacccgggcg aggtgcgcgc cggagctctg catcacttcg agcacttcgc
     5041 gcagctgatc ggtgagccgc acggtcggca gggggcgaac ccaggattcg gcgatcggcc
     5101 ggttgcggtg cttctccttg acctcaaggg catccttgag gtggaggtaa ccgatcatct
     5161 cgtcgtcgcg ggtgaccggg aagcgggaga acccggtccg gccggccgcc tcctcgacct
     5221 cggccggagt gacggtgatc ggcagggtca ccatggtttc gatcgggagc agaaccacgc
     5281 tggcatcccg ttcttcaaag gtcagggccc cggtcagcag ccgggtttcg tcggcgtcga
     5341 gcattccttc acgccgtgac tcctcgacca gcccggcgac ttcatcccgg gtgaaggcgc
     5401 tggtcacctc atcggaagga gtcacgccca gcagccgcag gaccccgttc gcggtcttgt
     5461 tgagcaccca gatgatcgga tgaagcacct tgaccaccgc ggcgatcggc gggccgagcg
     5521 ccaacgcgga acggtccggc ccggccaggg cgatgttctt cgggaccatc tcgccgagca
     5581 ccacatggaa gccgccgacc agaaacaggg cgaccaccag ggccgccgga tggaccagtg
     5641 acccggggat gcccaggccc tcgaaggggt gctcgagcgc gtgcgcgatc gccggctcgg
     5701 ccagggcgcc cagcccgagc gtgcagaggg tgatgccgag ctgggcgccg gccatcatca
     5761 gggctacccg ttccatcgcg tgaagggtga tccgcgccgc ccagccacct tcgtcggcgc
     5821 ggagctcgat caccgaccgg cgggcggaga tcagcgcgaa ctcggccccg acgaagaagg
     5881 cattgccggc cagcagcaag agagaaatca tcagggccag cggggtgctc acttctcgcc
     5941 ctccccatcc tcttccggct gaggcttcga gaccacgagc cgcacacggt cgaccctgag
     6001 cccgtccatc cgcagcacgt agaggtcggc cagaagcggc tccttctcct cgttggtcgc
     6061 ttcgaccctg accgagtcgc cgcggccggg cacccggccg agttcgaagc cgatcagccc
     6121 ggcgacggtt tcgtaatcct cgtcttcagg gagcctcacc ccgatctggt cttcgacctc
     6181 gtccggccgc atcagcccgg aaaccgccca gattccctgc gcgatttcac gcaccggatc
     6241 ctcgtcccgg tcatgctcgt cacgaacctc gccgacgatc tcctcgatca ggtcttcaag
     6301 cgtgacgatg ccgtcgaagc tgccgaactc gtcgaccacg atcgcgacct gcaggccgac
     6361 cttgcgcagt tcggccagca gcgggtcaag ctcgattgtc gaggggacca ggaccggatc
     6421 ggccatcacg tcgcggatcg gcacatcgtc gcggtcttca tgggggaccg cgaccgcgtg
     6481 tttgacatgg acgattccct cgacgatctc ggatccttcg ctgatcaccg ggaaacggga
     6541 aaagccaccg gcccgggccg cctcgattac cgcgatcacc ggttgatcgg gagccagggt
     6601 cttgaccttg acccgcggag tcatcacgtc gtctgccctg agctcgccga actcgagtga
     6661 cttctcgagc aactcggcgg tcggctcctc cagcgtcccc ttttcagccg agcggcggac
     6721 cagcgaggtc agctcctccg gtgaccgggc cgaggccagt tcttcctggg gctcgatccc
     6781 aacccggcgg aggatccagt tggcgatgcc gttggtcacc ctgaccagcg ggtggatgat
     6841 ccgcgtgaat ccgcgctgga aaccctggac cgccttggcc gtccccatcg cgttggcgat
     6901 cgcgaggttc ttgggcacca gctcgccgaa gatcatggtg acggcgttgg cgaggatgaa
     6961 ggcgaccgtc aaagcgacac cgtcaacggc gccggcagaa accccgagcg actccagcgg
     7021 ggcgtcgatc agcgaagcga ccgagggctg ggcgaggtaa ccgatgagca ggttggtgac
     7081 cgtgatcccg acctgggcgg ccgaaagctg ggttgaaagg gtccgcagcc cctgccggac
     7141 tccgcgggcc cggcggtcac cttcatcggc ctcgcggtca acccgggacc ggtccacggt
     7201 gacgaaggag aattcggcag cgacgaaggc tccgcaggcc acgacgaggg caacggaaac
     7261 gagtacgagc agcagctcga tcatcgaggc agcgctcccg gcacccggct caggaagcgc
     7321 ctgggactag aaccggggtc gtcgtccatc caggtttccg cgagacttca tccggttcga
     7381 agattacccc tcacgggcag atgcatcggt ccgatacccg ctccgatccc gctgatcgcg
     7441 ggtttactac cccagatacc ctttgtcagt ggcatccctc ctcagacgcc tgctttttgc
     7501 cgtgctcgcg gccacattcc tgatctcgtc gggggcgtcg gcggctgcgc cggaaccggt
     7561 ggttgacggc aaccagatcg tcgattcggc gaccggggat cagttcgtcc cgcacggggt
     7621 caattttccc ggctacgagt acgcctgcaa gcagggctgg aactacgggg aggaaggcac
     7681 ccgcccggcc gagatcgcgt cgaccgtggc cgggatcgca tcttggaaca tcaacaccgt
     7741 ccggctcccg ctgaaccagg actgctggct cggcgatgac gggctgcccg ccgggccggg
     7801 ccgcaccgtg gccggatacc gccagtcggt cgaggatttc gtcaacgccc tcaacgcggc
     7861 cggcatcgtc gcgatcgtcg acatccactg gtccgggccg gacggcgtcg tggccgacgg
     7921 cctgcgcccg atgcccgaca accgctcgcc cgccttctgg agttcggtcg caacgacttt
     7981 caaggacaac ccgtcggtca tcttcgacct cttcaacgag ccccattcgc gctggaacgc
     8041 cgatgacacc aaggtcttcg gcctgagctg ggactgctgg gcgaacggcg gctgcatggc
     8101 tccggccgag cgcgacctgg ccaaggtcag cgggctttcc tggtaccgca cggtcggcat
     8161 gaagaagctg accgaggtgg tcagggcgac cggagcgacc cagccgatcc tgctttccgg
     8221 gaccgattac gcgaatgacc tgcgtggctg gctggccaac gcgccggaag atgatcagct
     8281 gatcgccggt ttccacaact atctcgagca gcgctgccgg accgagacct gctgggaccg
     8341 cgaagttgct cccgtcgccg agcacgttcc ggtcctggcc gccgagttcg gtcagaacga
     8401 ttgcggaccg cccggccaca tgaaccgctt catggactgg gcagacgacc acctgatcgg
     8461 atacctggcc tgggcctggt ggtcgcttcc cgacgagggt tgccacaact tcgccctggt
     8521 ctccaacctg gacggcaccc cgctcgcccc ggcggggact gcgctgaaca gccatctcgc
     8581 cagccttcca cgggtgctgc cgcaaccggt caagcgggtc tcccccgggc tggtgatcaa
     8641 gcgggcgcag tggaagcgac ccaccctgcg gctccgggtc gcgatcaagc cgaatgcctc
     8701 ggcggcgatc acggtgcaaa tccggctggc ccgcgattcg cacgcacgcc gcgcgccccg
     8761 cacgaaccgg cgattgatca ccaaaaagct gaggaccggg ttcggcttcg tcgaccttca
     8821 caccgcgatt ccgaccgggc tcaaaccggt cctggtcacc gtttcctacc ccggtgacaa
     8881 gcggctgctg ccaaagacgg tgaagcgcaa acccggttct gtgtccaagc tcacccgtta
     8941 gaaagccgaa aagcgggaaa atagggacat gacggtacgc cagacgatcg agatccacgg
     9001 ccagccggtc acctaccaca agatgggcga aggcccgccg gtcctgctgg tccacggaat
     9061 cacctccagt tcgcgaacct ggaaatcggt gatgccgcgg ctggccgaga cgcacacggt
     9121 gatcgcgccg gacctgctcg ggcacggccg ctcggccaag ccccagggtg actactcgct
     9181 cggcgcctac gccagcggga tgcgtgacct gctggtcgcg cttgatgtgg agaaggtcaa
     9241 cgtggtcggc cattcactcg gcggcggcgt cgccatgcag ttcgcctacc agttccccga
     9301 tcgcatatcg cggctggtcc tggttgacac cggcgggatc ggccgggagg tcaatccggc
     9361 cctcagggcc gccgccctgc ccggagccga gttcgtcctg ccactgctct tcacgcccac
     9421 cctgcacgat gccgggctca aggtgagg
//
LOCUS       DAOQKN010000086         9416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_3464_length_9416_cov_5.612328, whole genome shotgun sequence.
ACCESSION   DAOQKN010000086 DAOQKN010000000
VERSION     DAOQKN010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 9416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9416
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3464_length_9416_cov_5.612328"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1218
                     /locus_tag="PLJ64_07120"
     CDS             <1..1218
                     /locus_tag="PLJ64_07120"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNC93287.1"
                     /translation="NGIVLPGFVNAHTHLTEGLITGMGETAVLWEWFERVVNPAGLRI
                     TRDEVALGTRLKAVEMLESGITTVNDMSCHRNLGSLASLGAVDGLAATGMRGVVSFGA
                     EDMYDGAPGADVFMAEHEALADRIAPEELIDFRIGVGTVLGMSDELFGRTIKAAGENG
                     WAVHTHLAEVREEITESTLLHGGSTIRHADRTGLLDHEVIAGHCIWCSETDIGLLASN
                     DVAVAHNPVANMILASGVCPVPRLLREGVRVGIGTDGAASNDSQDMFGAIKSAAVLQK
                     VHHLQAVAMTAPQVMEMATIGGAKALGKEKEIGSLEVGKRADVVLLDGNGPELAVLHD
                     PFQQIVYGATPRSVSEVWVDGRLRVTGAETVGVDRTALASEAREAARDLAIRGEMKGE
                     SAYVGALDFEEGK"
     gene            1222..2856
                     /locus_tag="PLJ64_07125"
     CDS             1222..2856
                     /locus_tag="PLJ64_07125"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNC93288.1"
                     /translation="MSETSDVVLIGAGHNGLVCGSYLARAGLSVTVLESSHTPGGCIY
                     TEDQPSGNRLELGAYEHGGIRGSGVAGDLELESKYGLVFHERDEFLFAPCDDGTGLPF
                     WNSLEQTVDALAGLLGSEEAERYRAFSKWSTAAMGVLGQTENGPPPSLRSLASIADLT
                     LGAEGARLMQALFAPATAVTEAALQDDRLRGPLDHWAAHSQQPPQAPGTGAGALFLAA
                     SHGQPAIRPAGGSRGTVDALVRCLEAAGGELIVNAPAAKVETAGGRATAVIAGGERYE
                     ASKAVISAIDARRLFLGLMDESQVPAPLLAEVRRIHFSQHNVSELKVDAVIGEVPRVN
                     GPDGMEKAFMLSANTGTDIARAFSSLGLGQLPERPPVMIAFPSTLESGWAPEGQGVVW
                     LSTFVPWTPAQGEWDQALLEEASELTWKTAEKALGWEMTAVEKKLTGPLQWVERHGNP
                     GANPNHIEMSIDQLMAMRPSVNLSGYSTPIEGLFLTGAGTHPGGGITGMPGRNAAGVI
                     LEQLGLARRKRGEKLKAQAALIKDALRATRELRKNA"
     gene            2853..>3801
                     /locus_tag="PLJ64_07130"
     CDS             2853..>3801
                     /locus_tag="PLJ64_07130"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNC93289.1"
                     /translation="MSKTGMDGSAGDPYAVADQFTSYQESALIGTAVEVGLPNLLYGS
                     PHNLEGITTALGTDPRGTLGLLSGLVTFGLVQREGETFSLTANGELLADEHPDSVAMI
                     ARKEWFFYKLWAEMPAAVRTGHTQTGAWRERLENDPDQAHDFLRALDDLCRLYGGDLP
                     ELAGLPQGPPARRLLDVGGGAGSHSANLVKAVEGLKATVLDLPGAEPVLRERHPELDF
                     VAGDIDLPRFGRPEGEQWDYVLVSNILHDRPAAENERIIREAAGLLAPGGSLVIYEWV
                     IDEGRTSPPGVASFTPMMVIENEGGWTWTESEIGEWIRSA"
     assembly_gap    3802..3901
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3902..4822)
                     /locus_tag="PLJ64_07135"
     CDS             complement(<3902..4822)
                     /locus_tag="PLJ64_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93290.1"
                     /translation="MADVRNYAKLTRRYAKFSLAGVLALLALVVLVIGGKPESAQAAP
                     VACPKFRVMNNDRIGKVSFPAGYYSVSLLNGNKLTCAQSTKLFQEFLQDWDGKLRSPW
                     RVYMSGNKRVFRAGNTDKGFTATPSGSSGGGGGGNSGRSCPSYFTVLHNDSIGSFKVP
                     RGKYRFTLIDPNKITCAQAAKRFQEFLLDFDGKLPYPWKLNPILGIFYNSLHGRIGFK
                     VNKAYGPSPSPKPDPSYGRCPATFRVLHNDRIGALSLPRGPYYIYVVNGLSCSSASNY
                     FRQFLNRTDGRLPAPWRLNVAKAKFNTGHGG"
     gene            4912..6666
                     /locus_tag="PLJ64_07140"
     CDS             4912..6666
                     /locus_tag="PLJ64_07140"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein DipZ"
                     /protein_id="HNC93291.1"
                     /translation="MALLILFGFIAGAATAVSPCVLPVLPVALSAGATGGRRRPAGIV
                     AGLTASFTFATVALVYVIDALGLPDDLIRNIAIAVLIGFGIVLLLPSVSARFEGWVSQ
                     FTARFTPQNSGDGFWSGTVVGASLGLVYAPCAGPILAGVITVSASQSFTVERFAIAFA
                     YALGSALVLYLLMLGGRRLITPLTKRVGWFQAAMGIVMIVFGIGMAAQWDLKFQNWIA
                     DDLPAIVVNPTKDLESSDATSSRLADLRAGPQSASAAKIAAGSAVVQQEARAASDLPV
                     IGKAPEFQGTQEWFNTPGDKPLTMAGLRGQVVLIDFWTYTCINCIRTQPWLNAMEERY
                     GDKGLTIVGVHTPEFPFEKDAGNVEDAIGRAGIKYPVVQDNDYTVWNSYGNQYWPAEY
                     FVDADGNVRYAHFGEGDYSHKEEVIRELLAEAGHDPGAGQTGATGMKAAKVLLTPESY
                     LGSARADRFENGTILPGVHDFGPLKEPKLDWLAYGGKWSISPEDARSDGGQIAVHFRA
                     RRVYLVLSSPGKARSAGIELDGKPVSAAAAGDDVKSGQVEVTSQRLYSLIDLPKPGEH
                     VLTVSPEAGISGYAFTFG"
     gene            6732..6805
                     /locus_tag="PLJ64_07145"
     tRNA            6732..6805
                     /locus_tag="PLJ64_07145"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6766..6768,aa:Arg,seq:acg)
     gene            complement(6960..7274)
                     /locus_tag="PLJ64_07150"
     CDS             complement(6960..7274)
                     /locus_tag="PLJ64_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histone"
                     /protein_id="HNC93292.1"
                     /translation="MTQTKAERSAAAKKAAATRKRNEAKAEAAASDRGKKAAATRQGK
                     AAKQSADQAKRSAGQAKQTAAEAKDSTGSAVGSLANAAKSAGDAALKAGKSVASRVRS
                     GF"
     gene            complement(7328..>9416)
                     /locus_tag="PLJ64_07155"
     CDS             complement(7328..>9416)
                     /locus_tag="PLJ64_07155"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93293.1"
                     /translation="GNYGGASRGGDGGGLVGTSGTSPTAGAPGTQTEGGAGTWPGSLG
                     QGGGGGGFGSGATRGGGGGGYYGGSTNDQEGGGGSGYGPSGTFFETGVRDGNGQAILT
                     YTTVRYALGVDILGAGQGHVSSSPAGIDCGYSGRIDCGENFAEATQVTLTATAEPGSI
                     FTGWSGACSGSSPTCNVSMTEARDVHSTFEQARTLSVTKSGTGAGSLTSNPAGIDCGS
                     ACSDDAAEFALGSSVTLTASPDIGSSFSFWSGGGCSGTSSTCTVTMSQARSVEATFTA
                     SELALTVVRKGNGAGTVTSQPAGISCGNTCNANYIYGTSVTLTASPGSDSTFGGWSGD
                     GCSGTSTMCTVSTDQARSVEATFALQKRTLTVSKAGNGSGLVTSGPAGIDCGSDCAAD
                     FDLGSSVTLVPDPAPGSDFVGWSGACTGSGAACIVTIDQARSVQAEFSLEKRTLTVTK
                     PGNGLGTVTSEPAGIVCGEDCTDEYDFGSSDTLTVTPAASSTFTGWQGACEGSGPECT
                     VTMDQARSAQALFTLRKHQLTVTTSGSGSGTVTSSPAGISCGSGCSADYDDGTPVILV
                     AAPAAGSTFTGWSGTCSGTGVLCTVTMTEARSVSAAFASIPPGTTITRTPKKKIRTKA
                     KRLTVRFAFRSSQAGSTFECRIDRRPFTSCRSPLRLKVKRGRHEFQVRARNAAGVADP
                     TPASYRFRILKKR"
BASE COUNT         1492 a         3106 c         3176 g         1542 t
ORIGIN      
        1 aacggcatcg tcctgcccgg cttcgtcaat gcccacaccc atctgaccga gggcctgatc
       61 acgggcatgg gcgagaccgc ggtgctctgg gaatggttcg agcgggtggt caaccccgcc
      121 ggcctgcgga tcacccgcga cgaggtcgcg ctgggcaccc gcctgaaggc ggtcgagatg
      181 ctggaatccg gcatcaccac ggtcaacgac atgagttgcc accgcaatct gggctcgttg
      241 gcatcgctcg gtgcggtcga cgggctcgcc gcgacgggca tgcgcggagt ggtcagcttc
      301 ggggccgagg acatgtacga cggggcgccg ggcgccgacg tcttcatggc cgagcacgaa
      361 gcgctcgccg accggatcgc cccggaagag ctgattgact tccggatcgg ggtcggcacc
      421 gtgctcggca tgagcgacga gctcttcgga cggacgatca aggccgccgg cgagaacggc
      481 tgggcggtgc acacccacct ggccgaggtg cgggaggaga tcaccgagtc gacgctgctc
      541 catggcgggt cgaccatccg gcacgccgac cggaccgggc tgctcgacca cgaggtgatc
      601 gccggccact gcatctggtg ctcggagacc gacatcggcc tgctcgcctc caacgacgtc
      661 gccgtggctc acaacccggt cgccaacatg atcctcgcct ccggagtctg cccggtcccc
      721 cggctgctcc gcgaaggggt gcgggtcggg atcggcaccg acggtgccgc cagcaacgac
      781 tcccaggaca tgttcggggc gatcaagtcc gccgccgtgc tgcagaaggt ccatcatctc
      841 caggcggtcg cgatgaccgc gccgcaggtg atggaaatgg cgacgatcgg cggtgcgaag
      901 gcactcggca aggaaaagga gatcggcagc ctggaggtcg gcaaacgggc cgacgtggtg
      961 ctgctcgacg gcaacgggcc cgagctggcc gtgctgcacg atcctttcca gcagatcgtc
     1021 tatggcgcta ctccgcgcag cgtctccgag gtctgggtcg acggcaggct ccgggtaacc
     1081 ggggcggaaa ccgtcggcgt ggaccgaacg gccctagcct cggaagcccg ggaagctgcc
     1141 cgggacctgg cgattcgcgg cgagatgaag ggcgagtcgg cctacgtcgg cgcgctcgat
     1201 tttgaagaag ggaagtaagc gatgtcggaa accagtgacg tcgtcctgat cggggccggc
     1261 cacaacgggc tggtctgtgg ttcctacctg gcccgggccg ggctctcggt caccgtgctc
     1321 gagtcgtccc atacgccggg cggctgcatc tacaccgagg accagccttc cggaaaccgt
     1381 ctcgagctcg gcgcctacga gcacggcggc atccgcggca gcggcgtcgc cggggacctc
     1441 gagctcgaat cgaagtacgg gctggtcttc cacgagcgcg acgagttcct cttcgctccc
     1501 tgtgacgatg ggaccggcct gcccttctgg aactccctcg agcagacggt cgatgccctc
     1561 gccgggttgc tcggcagtga agaggccgag cgttaccgcg ccttctcgaa atggtcgacc
     1621 gccgcgatgg gggtgctcgg ccagaccgag aacggcccgc cgccgagcct gcgttcgctc
     1681 gcttcgatcg ccgacctgac cctcggggcc gagggtgccc gcctgatgca ggccctcttt
     1741 gcccccgcca ccgcggtcac cgaagcggcc cttcaggatg accgcctgcg tggtccgctc
     1801 gatcactggg ccgcccattc acagcagccg ccgcaggctc ccggcaccgg cgccggcgcc
     1861 ctcttcctcg ccgcctcaca cggccagccg gcgatccgcc ccgccggagg ctcaagggga
     1921 accgtcgatg cgctggtccg ctgcctggaa gctgccggcg gcgagctgat cgtgaacgcg
     1981 ccggccgcga aggtcgagac cgccggcggc cgggccaccg cggtgattgc gggcggcgag
     2041 cgctacgaag cgagcaaggc ggtcatctcg gcgatcgacg cccgccgtct tttcctcggc
     2101 ctgatggacg agagtcaggt gccggcaccg ctgctggccg aggtgcgccg gattcacttc
     2161 agccagcaca acgtttccga actcaaggtc gatgcggtga tcggcgaggt cccgcgggtc
     2221 aacggtcctg acggaatgga gaaggccttc atgctctccg ccaacaccgg caccgacatc
     2281 gccagggctt tctccagcct cggtctcggc caactgcccg aacggccacc ggtgatgatc
     2341 gccttccctt cgaccctgga atccggctgg gccccggaag gtcagggcgt ggtctggctt
     2401 tcaaccttcg tgccctggac ccccgcgcag ggcgagtggg accaggccct cctggaggag
     2461 gcctccgagc tgacctggaa gacggcagag aaagcgctcg gctgggagat gaccgcggtc
     2521 gagaagaaac tgaccgggcc gctccagtgg gtcgagcggc acggcaaccc gggcgccaat
     2581 cccaatcaca tcgaaatgag catcgaccag ttgatggcga tgcggccctc ggtcaacctt
     2641 tccggctaca gcaccccgat cgaaggtctc ttcctgaccg gggccggtac ccacccgggc
     2701 ggcgggatca ccggcatgcc ggggaggaac gcggccgggg tgatcctcga gcagcttggc
     2761 ctcgccaggc ggaagcgtgg tgaaaagctg aaggcccagg cggccctgat caaagacgcg
     2821 ctgcgcgcaa ccagagaact gaggaagaac gcatgagcaa aaccgggatg gacggcagcg
     2881 cgggcgaccc ctatgcggtc gccgaccagt tcacctccta ccaggagtcg gccctgatcg
     2941 ggaccgcggt cgaggtcggc ctgccgaacc ttctttacgg ctcaccccac aacctggagg
     3001 ggatcacgac cgcgctcgga accgacccgc gcggcacctt gggtctgctt tccgggctgg
     3061 tcaccttcgg cctcgtccag cgtgaaggcg agaccttcag cctgaccgcg aacggcgagc
     3121 tcctggccga cgagcacccc gactcggtcg ccatgatcgc cagaaaagaa tggttcttct
     3181 acaagctctg ggccgagatg ccggccgcgg tgcggaccgg ccacacccag accggcgcct
     3241 ggcgtgagcg actcgagaac gaccccgatc aggcccacga tttcctccgc gccctcgatg
     3301 acctctgccg cctctacggc ggcgatctgc cggagctggc cggcctgccg cagggcccgc
     3361 ccgcccgccg cctgctcgat gtcggcggcg gtgccggttc gcattcggcc aacctggtca
     3421 aggcggtcga aggcctcaag gcaacggttc tcgacttgcc gggggccgag ccggtcctca
     3481 gggaacgcca tccggaactc gacttcgtcg ccggtgacat cgacctgccc cgtttcggcc
     3541 ggccggaggg ggagcagtgg gactacgtgc tcgtctcgaa catcctgcac gaccgtccgg
     3601 cggcggagaa cgagcgcatc atccgggaag cggccgggct gctcgccccg ggcggctcac
     3661 tggtcatcta cgaatgggtg atcgacgagg gccgtacctc gccacccggc gtcgcctcgt
     3721 tcaccccgat gatggtgatc gaaaacgaag gcggctggac ctggaccgaa tccgaaatag
     3781 gcgagtggat ccggtcagcc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 ngccgccgtg gccggtgttg aacttggcct tggccacgtt gagacgccac ggggcgggca
     3961 ggcggccgtc ggtccggttg aggaactggc ggaagtagtt cgaggcgctg gagcagctca
     4021 gtccgttgac cacgtagatg tagtacgggc cgcgcggcag tgagagtgcg ccgatccggt
     4081 cgttgtgaag gacccggaag gtggcggggc agcgaccgta ggaggggtcg ggcttcgggc
     4141 tcggcgaggg gccgtaggcc ttgttcacct tgaagccgat ccggccgtgg aggctgttgt
     4201 agaagatgcc gaggatcggg ttgagcttcc acgggtaggg gagcttgccg tcgaagtcga
     4261 gcaggaactc ctggaagcgc ttggccgcct gggcgcaggt gatcttgttc gggtcgatca
     4321 gcgtgaagcg gtacttgcct cgtggcacct tgaaactgcc gatcgagtca ttgtgcagca
     4381 ccgtgaagta gctggggcag gagcggcccg agttgccgcc gcctccaccg ccgctggagc
     4441 cggagggagt cgcggtgaag cccttgtcgg tgttgccggc ccggaagacc cgcttgttac
     4501 cgctcatgta gacacgccag gggctgcgca gcttgccatc ccagtcctgc aggaactcct
     4561 ggaaaagttt ggtcgactgg gcgcaggtca gcttgttgcc gttcagcagg ctgaccgagt
     4621 agtaaccggc cgggaatgaa accttgccga tccggtcgtt gttcatgacc cggaacttgg
     4681 ggcaggcaac cggggctgcc tgggcgctct cgggcttgcc tccgatcacg aggaccacga
     4741 gggccagcag ggcaagcact ccggcgaggg agaacttggc gtatcgacgg gtcagtttcg
     4801 cgtaattccg tacatcggcc atatgaagaa gaaccctact tccgcagtca agttccggcc
     4861 atcccggaac tatttgtgat ccaaagccgc tccgtttgcg aatcttcagg catggccctg
     4921 ctgattttgt tcgggttcat cgccggggcg gcgaccgctg tctcgccctg cgtgctgcca
     4981 gtgctgccgg tagccctctc ggccggggcg acgggcggca ggcgccggcc ggccgggatc
     5041 gtggccgggc tcaccgcctc cttcaccttc gccacggtcg ccctggtcta cgtgatcgat
     5101 gccctcggcc tgcccgatga cctgatccgc aacatcgcga tagcggtcct gatcggtttc
     5161 gggatcgtgc ttctgctgcc ctccgtctcg gcccgcttcg aaggctgggt cagccagttc
     5221 actgcgcgct tcacaccgca gaactcgggt gacggcttct ggtcgggcac ggtggttggc
     5281 gccagcctcg gtctcgtcta cgccccttgc gccggcccga tcctcgccgg cgtgattacc
     5341 gtctcggcct cgcaatcgtt caccgtcgag cgattcgcga tcgccttcgc ttatgcgctc
     5401 gggtcggccc tcgtgcttta tctcctgatg ctcggtggtc gtcggctgat cacgccgttg
     5461 acgaaacggg tcggctggtt ccaggcggcg atggggatcg tgatgatcgt cttcgggatc
     5521 ggcatggccg cccagtggga cctcaagttc cagaattgga tcgccgacga cctgccggcc
     5581 attgtggtca acccgaccaa ggacctcgag tcgagtgatg ccacttcgag ccggctggcc
     5641 gacctgaggg ccggccccca gtcggcctcc gccgcgaaga tcgccgccgg gtcggcggtg
     5701 gtgcagcagg aagcccgggc cgcctcggat ctcccggtga ttggcaaggc tccggagttc
     5761 cagggcaccc aggagtggtt caacacgccg ggcgacaagc cgctgaccat ggccgggttg
     5821 cgaggccagg tcgtgctgat cgacttctgg acctacacct gcatcaactg catccgcacc
     5881 cagccctggc tcaacgcgat ggaggagcgt tacggggaca agggcctgac catcgtcggg
     5941 gtccacaccc ccgagttccc tttcgaaaag gacgccggca acgtcgagga tgcgatcggg
     6001 cgggcaggca tcaagtaccc ggtggtccag gacaacgact acacggtctg gaactcctac
     6061 ggcaaccagt actggccggc cgagtatttc gtcgacgccg acggcaacgt ccgctacgcc
     6121 cacttcggcg agggcgacta cagccacaag gaagaagtga tccgcgaact actggccgag
     6181 gccggtcacg atcccggcgc agggcagacg ggcgcgaccg gcatgaaggc ggccaaggtc
     6241 ctcctgaccc cggagagcta tctcggttcg gcccgggccg accgcttcga gaacggaacg
     6301 atcctgcccg gcgttcacga cttcgggccc ctgaaggagc cgaagctcga ctggctcgcc
     6361 tacggcggca agtggagcat cagcccggag gacgcacggt ccgacggcgg ccagatcgcg
     6421 gtccacttcc gggccaggcg ggtctatctg gtcctttcct caccgggcaa ggcccgcagc
     6481 gccgggatcg aactggacgg caagccggtc agtgccgcgg cggccggaga tgacgtgaaa
     6541 agcggtcagg tcgaggtcac gagccagagg ctctacagcc tgatcgacct gccgaaaccg
     6601 ggcgagcacg tgctgacggt cagcccggaa gcgggcatca gcggctacgc cttcaccttc
     6661 ggctgagcca gcaggagtcc cgaacgctcg caacctcgta gaatcgccga tccggggtgt
     6721 ttccccaatc cgcgcccgta gctcagctgg atagagcgtt ggtctacgaa accaaaggtc
     6781 acaggttcga atcctgtcgg gcgcgcttgc tggcggggca gccttcgcgc taccagccgc
     6841 ctgttcccgg gtcgcctttg atcgggccgg tgaagccgca ggttcgtgaa caggttcgga
     6901 ccctcgttca cctgggcttt gacgccccag gcgttggcga gggcccgggc cccggggatc
     6961 tagaagccgc tgcggacgcg ggaagcgacc gacttgccgg ccttgagggc ggcgtcaccg
     7021 gccgacttgg ccgcgttggc gaggctgccg acggccgatc cggtcgaatc tttggcctcg
     7081 gcggctgtct gcttcgcctg cccggccgac cgcttggcct gatcggcgga ctgcttggct
     7141 gccttgccct gacgggtagc ggcggccttc ttgccacggt cgctggcggc cgcttcggcc
     7201 ttggcttcgt ttcgcttcct tgtggcagct gccttctttg cggcggcgct gcgttcagct
     7261 ttggtctgag tcatgacact ccggtacccg gaccaaaaac cttcaatcgt gaggaatcga
     7321 ctccgggtta ccgcttcttc aaaatcctga agcggtaact ggccggggtc gggtcggcta
     7381 ctccggctgc gttgcgggcg cgaacctgga actcgtgcct gcctcgcttg accttgagcc
     7441 ggagcgggga gcggcaggaa gtgaacggcc ggcggtcgat cctgcattcg aaggtcgagc
     7501 cggcctggct ggacctgaag gcgaacctca ccgtcaatcg cttcgccttc gtccggatct
     7561 tcttcttcgg ggttctcgtg atcgtggtcc cgggcgggat cgaggcgaaa gcggcgctga
     7621 cggaacgagc ctcggtcatc gtcacggtgc agagaacgcc ggtaccggag caggttccgc
     7681 tccagcccgt gaaggtcgaa ccggcggcgg gggcggcgac caggatcacg ggcgtaccgt
     7741 cgtcgtagtc cgcgctgcag ccggatccgc acgagatccc ggccggcgac gaggtgacgg
     7801 tgccactccc ggagccggac gtggtcaccg tgagttggtg cttgcgaagc gtgaacaacg
     7861 cctgcgcgct tcgggcctga tccatcgtca cggtgcattc cggcccactt ccttcgcatg
     7921 ctccctgcca accagtgaag gtggagctgg cggccggcgt cacggtcaag gtgtccgaac
     7981 tcccgaagtc gtactcgtcg gtgcagtcct caccgcagac gatcccggcc ggttcggagg
     8041 tgacggtgcc gaggccgttg cctggtttgg tgaccgtgag ggtccgcttc tcgagcgaga
     8101 actccgcctg gacgctgcgc gcctggtcga tcgtcacgat gcacgccgcg ccgctgccgg
     8161 tgcaggcgcc gctccagccc acgaagtcgg aaccgggcgc cgggtcgggc acgagcgtga
     8221 cggaacttcc gaggtcgaag tccgccgcgc agtcggatcc gcagtcgatg ccggccggtc
     8281 cggaggtgac cagccccgag ccgttgcccg ccttgctcac cgtcagcgtt cgtttctgaa
     8341 gtgcgaaggt cgcctcgacg ctccgggcct gatcggtgct cacggtgcac atggtcgacg
     8401 tacccgaaca gccatcaccg ctccaacctc cgaacgtcga gtcactccct ggcgacgccg
     8461 tgagagtcac cgaagtcccg taaatgtagt tggcgttgca ggtgttgcca cagctgatcc
     8521 cggccggctg cgaggtgacg gtgccggcgc cgttgccctt cctgaccacg gtcagtgcga
     8581 gttcgctcgc ggtgaaggtc gcctcgacgc tgcgggcctg gctcatcgtc accgtgcagg
     8641 tcgaagaggt tccggagcag ccgccgccac tccagaagga aaagctggaa ccgatgtccg
     8701 gactggcggt caaggtgacc gagctgccca gggcgaactc ggctgcgtcg tcgctgcagg
     8761 cagagccaca atcgattccg gcagggttgg aggtgaggct gcccgcgccg gtgcctgact
     8821 tggtcaccga gagagtgcgg gcctgttcga aagtcgaatg gacgtcgcgg gcctcggtca
     8881 tgctcacgtt gcaggtgggc gaacttcccg aacaggcgcc gctccagccg gtgaagatcg
     8941 agccgggctc ggccgtcgcc gtcagcgtca cctgcgtcgc ctccgcgaag ttttcgccgc
     9001 agtcgatgcg accgctgtag ccgcaatcga tgccggcggg cgaagaactt acgtgcccct
     9061 gcccggcacc gaggatgtcc acgcccagcg cgtacctgac cgtggtgtag gtgaggatcg
     9121 cctggccgtt gccgtcgcga acgccggtct cgaagaaagt gccggacggc ccgtacccgg
     9181 agccaccgcc gccctcctgg tcattggtgc tgccgccgta atagccaccg ccaccaccgc
     9241 cgcgcgtcgc ccccgaaccg aatcctcctc cgccgccgcc ctggccgagc gaccccggcc
     9301 aggtgccggc tccgccttcg gtctgggtgc cgggggcacc ggcggtcggt gaggtgccgc
     9361 tcgtgccgac gagtccgccg ccatcgccgc cgcgagaggc accgccgtaa tttccg
//
LOCUS       DAOQKN010000087         9413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_3466_length_9413_cov_6.053430, whole genome shotgun sequence.
ACCESSION   DAOQKN010000087 DAOQKN010000000
VERSION     DAOQKN010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 9413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9413
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3466_length_9413_cov_6.053430"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="PLJ64_07160"
     CDS             <1..187
                     /locus_tag="PLJ64_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93294.1"
                     /translation="SVLGPIHGFFFVVLIGLCAYGSLEKWWGWWFPILTLVTGGAIGS
                     LIGDLVVRRQLRERAAA"
     gene            184..2004
                     /locus_tag="PLJ64_07165"
     CDS             184..2004
                     /locus_tag="PLJ64_07165"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HNC93295.1"
                     /translation="MSDSGTALAEVTWNLDDLLEGDHADPEAAVTALLDEAEKASEKF
                     AAEYEGKVSELDGPGLIVAMTKLADISDRAGRALNYAHLRFAADTADPANGALLQMGS
                     ERATRIQTQLLFFDLEWAALDDEVAEALLATPGLEFCEHHLRNERKYRDHLLSAPEEK
                     IVTELSVTGAGAWSRLFDELTSAIRVNLPGGEEPVSLDIALSGLHNPDREVRKATSEA
                     VTESLEPGLRTRAYVFNTLLQNKATMDRLREYPHWLATRNLSNEASDESVQALVQAVK
                     GRYDLARSWYRTKAKLLEVDRLADYDRMAAIATDDVTIEWEEGRQIVQSAFDSLAPQA
                     GEVVGRFFEDRWIDAPPAPSKRGGAFSASTVPSVHPYVMLNYTDRRRDVLTLAHELGH
                     GLHQALAAKQGIFHQDTPLTVAETASVFAEELVFGRLLAAEKDPASRLGLLSESVEGQ
                     IATIFRQIAMNQFEDAVHTARREEGELSVERFGELWAATQEDLLGDSVEVTDGYRSWW
                     SYVPHFIGSPGYVYAYAYGQLLALSVYRLYEEQGEAIVPGYLEMLTAGGSRSPEQLGA
                     LVGVDLSDPSFWDNGLDLVAGQIDAAQDAAEEVLRARSQG"
     gene            2118..2669
                     /locus_tag="PLJ64_07170"
     CDS             2118..2669
                     /locus_tag="PLJ64_07170"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC93296.1"
                     /translation="MPEQSRKPRVSQEARRAEVIDAALIEFGRCGYAGTSTSMIANRA
                     GIRQPYIYALFENKRALFLACHEVLNDRIRETFRNAALPEDSPYERVRKMGLAYLGLL
                     HDDDRVRCHLQVFAAAGSDDLKEPIREGFNRLFEDVLEMSEASRPEVARFFATGMLLN
                     AMAALDEPFEMIRYLEVTPEDEL"
     gene            2666..3388
                     /locus_tag="PLJ64_07175"
     CDS             2666..3388
                     /locus_tag="PLJ64_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93297.1"
                     /translation="MTGPHPRLTALLVTGILLFPVAAGAKYQKTQPVGWGPRTSKAAA
                     KLVKRNGYEPRPINYRDNHRVPGEKLLRAWRKRSDMPYKRFVDGRFKGTTDEIIQWAS
                     LKWGIDTDTMRAAAVVESWWDMDVRGDNGDSFGLYQVRRPYHCWGKCKIARYFTAFNA
                     DYYGAIIRSYYDGKQTWLNTVSGNGKKYRAGDLEGSMGAWYSGRWWGPPERPIEPYLE
                     KIARQKADRTWRHADFIAYRQP"
     gene            complement(3450..5507)
                     /locus_tag="PLJ64_07180"
     CDS             complement(3450..5507)
                     /locus_tag="PLJ64_07180"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HNC93298.1"
                     /translation="MGALATFAFACLLMASPSLASAGWTSPATVSGPGTSVSQTTVAG
                     GSDGTSWVVWKRDVGGFNLIQGTRVQIDGTQGPIVTLSPVGQNANNPVIASRADGSAV
                     VGWTNESGTSDFIQSVSIAADGTVGAVTDRSVNGPAGQDASDLAIAVGADGTAGLAWL
                     RFDGTEPVVQAVRVASDGSSGTVHNLSAASAAPGPPDVGAAPPTTAGDPYFYRVMWPQ
                     GSAATSQVANADVMANDTVTTTVLLYTNASGADCADPFDIQIAYGSDGAMNAFWVCYK
                     KTIDFNTFNTFYNWSVQWLRTAKGVPLSTGGAASATNASPTIYLSPYPISGVVAAESF
                     TGQPAIAWIHDLDGGGQRVETWRIMINVLNGVPLPVGQGWANTTTEATAVGSPAIAVN
                     SNNTGIAGGVELGALPGQNTTNFTRFSNSSYDPVTPSGGFTYSEDPGYVLTDGGKTLA
                     AFTGITGPVGSAMVMTFEDPGISVDPDSFNFGHADIGTKRSVSISIRSTGQTTNTVTG
                     ITLGGANADRYAMSGADDCIREMTPNTNCRFTVTFTPGSTASQTAQISVTSDGGNEVA
                     NLTGTGLNRTRNSITAKPGHAARRKGKVVRIRVKASNLGGVSSNNTRICVNLRKRALK
                     LAGNRCRNLGTLGVGATRNLNYRIRVTWRAQRGVKLPVTFVLRSNNTVVRQAVVQLRR
                     KGR"
     gene            complement(5647..8277)
                     /locus_tag="PLJ64_07185"
     CDS             complement(5647..8277)
                     /locus_tag="PLJ64_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HNC93299.1"
                     /translation="MFERFTERARQVVVLAQEEARTLKHNYIGTEHILLGLLREEEGL
                     AARVLESLEITVERVRAQVVRIVGSGEEVTSGQIPFTPRAKKVLELALREALSLGHNY
                     IGTEHILLGLVRENEGVAARILLDFDADSDKIRNEVIRMLSGPGGRRPGQSGSGSSSG
                     SSQGEGSGKKSSKLLDQFGRNLTKLAEDGKLDPVVGRETEIERIMQILSRRTKNNPVL
                     LGEPGVGKTAVVEGLAARIIKGEVPEMLRGKQIYTLDLAALVAGSKYRGEFEERLKKV
                     MKEIASRGDIVLFIDEIHNLVGAGAAEGAIDAASILKPALARGEIQTIGATTLDEYRK
                     YLERDSALERRFQQIRVEQPTPEQTVKILEGLRERYEQHHKVQISDDALEAAAELADR
                     YISDRFLPDKAIDLIDEAASRMRIKSMTAPPVYRDLEEEIETARREKEAAIEAQEFEK
                     AANLRDTERQLTTRKRQLEEEWAEGEEGERPVLGEEEIADIVSMWTGIPVFKLTEAET
                     KKLIQMESELHKRVIGQEAAIEAVSKAIRRSRAGIKDPKRPAGSFVFLGPSGVGKTEL
                     ARTLAEFLFGDEESMVRIDMSEYMEKHAVSRLVGSPPGYVGYDEGGQLTEAVRRKPYS
                     VLLLDEIEKAHPDVFNILLQILEDGRLTDSQGRTVDFRNAIIIMTSNIGASEIARNQG
                     IGFTVSDDTGITYDDMKGRIMSELRKVFRPEFLNRIDEVIVFHKLSREEVREIIELML
                     ARVRNQVAEHELMLELDNGAKDFLADKGWDPSMGARPLRRAIQRYIEDPLADEVLKAG
                     GGDSVAGSLVTVTRDESGDDDDKPLKLKITKPRKPRKKKEDGQEKVGVGAGSTDSPSS
                     EPPAVKPDVADGPAAESAAD"
     gene            8527..9153
                     /locus_tag="PLJ64_07190"
     CDS             8527..9153
                     /locus_tag="PLJ64_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93300.1"
                     /translation="MREHKRGVEFRWLPILAAALVSLALVIPMLAPTPSGAAYPSSFR
                     RHQEQYWVWYAPRSWIASSGKYDLNISSATGTLWNKYGYSAVVCYNPAAWFKYLRNNY
                     RDTAGAGFGIYSKPLSRTHFTRIGRIKQLPKARYGPAYFRQKLLWTGRRKGSGQVIRG
                     EMIMDIFAIDSFGNCAQRFQTRGAPARGNAASIRLLRLVQSTITQRNL"
     gene            9187..>9413
                     /locus_tag="PLJ64_07195"
     CDS             9187..>9413
                     /locus_tag="PLJ64_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93301.1"
                     /translation="MKRSSVVTFALALATVAALLAPASPASARDEVHRCTNWSWTGPS
                     SWDAVCSKQGISISSPDGRRGIDWGFSNIFCV"
BASE COUNT         1565 a         3061 c         3088 g         1699 t
ORIGIN      
        1 ctcggtgctc gggccgatcc acggcttctt cttcgtggtc ctgatcgggc tctgcgccta
       61 cgggtcactc gagaagtggt ggggctggtg gttcccgatc ctgaccctgg tcaccggcgg
      121 agcgatcggc tccctgatcg gagacctggt cgttcggcgc cagctcaggg aaagggcggc
      181 agcatgagcg attccggcac cgccctggcc gaagtcacct ggaacctcga cgacctgctc
      241 gaaggcgacc acgccgaccc ggaggcagcg gtcacggcgc tgctcgacga agcggagaag
      301 gcttcggaga agttcgcagc cgagtacgaa ggcaaggtct ccgagctgga cggtcccggc
      361 ctgatcgtcg ccatgaccaa actggccgac atctccgacc gggccggccg ggcgctcaac
      421 tacgcccatc tgcgcttcgc ggccgacacc gccgatccgg ccaacggcgc cctgctccag
      481 atgggctcgg agcgggcaac ccggatccag acccagctgc tcttcttcga cctcgagtgg
      541 gccgccctcg atgacgaggt ggccgaagcg ctgctggcca caccgggtct cgagttctgc
      601 gagcaccacc tgagaaacga gcgcaagtac cgcgaccatc tgctgagcgc cccggaagaa
      661 aagatcgtga ccgaactctc ggtcacgggc gccggagcct ggtcccggct cttcgacgag
      721 ctcacctcgg cgatccgggt caacctgccc ggtggagagg aaccggtcag cctcgacatc
      781 gccctttcgg gacttcacaa ccccgaccgt gaagtccgca aggcgacctc cgaggcggtg
      841 accgagtcgc tggaacccgg actaaggacg cgggcctacg tcttcaatac cctgcttcag
      901 aacaaggcga ccatggaccg gctgcgtgag tatccgcatt ggctggccac acgcaacctc
      961 agcaacgaag cttcggacga atcggtccag gccctggtcc aggcggtgaa gggccgctac
     1021 gacctcgccc gttcctggta ccggaccaag gcgaaactgc tggaagtcga tcgtctcgcc
     1081 gactacgacc gcatggcggc gatcgccacc gatgacgtga cgatcgagtg ggaggaaggc
     1141 cgccagatcg tccagagcgc cttcgattcg cttgccccgc aggccggtga ggtcgtggga
     1201 cgcttcttcg aggaccgctg gattgacgcg ccgccggccc cgagcaagcg aggcggcgcc
     1261 ttctcggctt cgacggtgcc ttcggttcat ccctacgtga tgctgaacta caccgaccgg
     1321 cgccgggacg tgctcacgct ggcccacgag ctcggccacg gccttcacca ggcgctggcg
     1381 gcaaagcagg gcatcttcca ccaggacacg ccgctgaccg tggccgaaac tgcttcggtc
     1441 ttcgccgagg aactggtctt cggccggttg ctggcggccg agaaggatcc ggcttcccgg
     1501 ctcggcctgc tttccgaatc ggtcgaaggc cagatcgcga cgatcttccg gcagatcgcg
     1561 atgaaccagt tcgaggacgc ggtccacacc gcccgccgcg aggaaggcga gctttcggtt
     1621 gagcggttcg gcgagctctg ggccgcgacc caggaggatc tgctcggaga ttccgtcgaa
     1681 gtgaccgacg gctaccgctc ctggtggtcg tatgtgcccc acttcatcgg gtcccccggt
     1741 tacgtctacg cctacgccta cggccagctg ctcgcgctct cggtctaccg cctctacgag
     1801 gaacagggcg aagcgatcgt gcccggttat ctggagatgc tgaccgccgg cggctcccgt
     1861 tccccagagc aactcggggc gctggtcggg gttgatctca gtgacccctc gttctgggac
     1921 aacggattgg atctggtggc aggacagatc gacgcagcgc aggacgcagc agaagaagtg
     1981 cttcgggccc gaagccaggg ttgatcgaca tctgacccgt cttttgcacc ggcgctcttt
     2041 ccggcggacc ggccgaaggg tgcgaaaaca ttggatccgg gtaaaggacg gttacccggt
     2101 tgaccgaagg gccagggatg ccagagcaat cgagaaaacc gagggtttca caggaagctc
     2161 ggcgagccga ggtcatcgac gcggccctga tcgagttcgg gcgctgcggc tacgccggca
     2221 cttcaacctc gatgattgcc aaccgggccg gaatccgcca gccgtacatc tacgccctgt
     2281 tcgagaacaa gcgggccctc tttctcgcct gtcacgaagt cctcaacgac cggatccggg
     2341 aaactttccg gaacgcggcc ctgcccgagg acagtcccta tgaacgagtc cggaagatgg
     2401 gactcgccta tctcggcctg cttcacgatg acgaccgggt ccgctgccac ctccaggtct
     2461 ttgccgcggc cggcagcgac gacctgaagg aaccgatccg ggaagggttc aaccggctct
     2521 ttgaagacgt gctcgagatg agcgaggcga gccggcccga agtggcccgc ttcttcgcga
     2581 ccggcatgct gctcaacgcg atggctgccc tggacgaacc gttcgagatg atccgctatc
     2641 tggaagtcac cccggaggac gaactttgac cggccctcac cccagactca cggccctgct
     2701 ggtcaccggg atcctgctct tcccggtcgc ggccggagcg aaataccaga agacccagcc
     2761 ggtcgggtgg ggtccgcgaa ccagcaaggc agccgcgaag ctggtcaagc gcaacggcta
     2821 cgagccgcgc ccgatcaact accgggacaa tcaccgggta ccaggcgaaa agctgctccg
     2881 cgcctggcgc aagcgcagcg acatgccgta caagcgtttc gtcgacggcc gcttcaaggg
     2941 cacgaccgac gagatcatcc agtgggcctc cctcaagtgg ggcattgata ccgacacgat
     3001 gcgcgccgcc gcggtggtcg aatcctggtg ggacatggac gtgcgcggcg acaacggaga
     3061 ttcattcggc ctctatcagg tgcgccggcc ctaccactgc tggggcaaat gcaagatcgc
     3121 ccgatacttc accgccttca acgccgacta ctacggcgcg atcatccgtt cctactacga
     3181 cggcaagcag acctggctga acaccgtttc cggcaacggc aagaagtacc gggccgggga
     3241 tctcgagggc agcatgggcg cctggtactc gggacgctgg tggggcccgc cggaacgccc
     3301 gatcgagcca tacctcgaga agatcgcccg gcagaaggcc gaccggacct ggcgccacgc
     3361 ggacttcatc gcctaccggc agccctaggg cctgccggaa atgaacctgc cacccgcggc
     3421 aggttccgaa tccgccaggg agtttttgcc tagcggccct tgcggcgaag ctgaaccacg
     3481 gcctgccgca ccacggtgtt gttcgagcgc aggacgaagg tgacgggaag cttgacgcca
     3541 cgctgggcgc gccaggtcac ccggatccgg taattgagat ttctggtcgc cccgaccccg
     3601 agcgttccca ggttgcggca gcggttgccg gccagcttca gagcccgctt gcggaggttc
     3661 acgcaaatcc tcgtgttgtt cgacgatacc ccgcccaggt tcgaggcctt gaccctgatc
     3721 cggacgacct tgcccttgcg tcgggcggcg tggccgggct ttgccgtgat cgagttccgg
     3781 gtccggttga ggccggtgcc ggtcaggttg gcgacctcgt ttccgccgtc cgaggtaacc
     3841 gaaatctgtg cggtctggct cgcggtcgaa ccgggcgtga aagtcaccgt gaagcggcaa
     3901 ttggtgttgg gggtcatttc cctgatgcag tcgtcggctc cgctcatcgc gtaacgatcg
     3961 gcgttggctc cgcccagggt gatcccggtg accgtgttgg tggtctgccc cgtgctgcgg
     4021 atcgagatcg agacgctccg cttcgtaccg atgtcggcat ggccgaagtt gaagctgtcc
     4081 ggatccacgc tgatcccggg gtcttcgaag gtcatcacca tggccgaccc gaccggaccg
     4141 gtgattccgg tgaaggcggc cagtgtcttg ccgccgtcgg tcagtacgta acccggatct
     4201 tcgctgtagg tgaagccacc ggagggcgtc accgggtcat aggaactgtt cgagaaccgg
     4261 gtgaaattgg tggtgttctg ccccggcagc gctcccagct cgacgccacc cgcgatgccg
     4321 gtgttattcg agttgacggc gatcgccggg gagccgaccg cggtcgcttc ggtggtcgtg
     4381 ttggcccagc cctggccgac cggaagcgga accccgttca gcacgttgat catgatccgc
     4441 caggtctcga cccgctggcc gccgccgtca aggtcgtgga tccaggcgat ggccggctgc
     4501 ccggtgaagg actcggccgc gacgacgcca ctgatcgggt acgggctcag atagatggtc
     4561 ggtgaggcgt tggtggctga agccgcccct cccgtgctga gtggcacccc cttggccgta
     4621 cgcagccact ggaccgacca gttgtagaag gtgttgaagg tgttgaaatc gatcgtcttc
     4681 ttgtaacaga cccagaaggc gttcatggct ccgtcggagc cgtaggcgat ctgaatgtcg
     4741 aagggatcgg cacagtccgc cccgctcgca ttcgtgtaca ggaggacggt ggtggtgacc
     4801 gtgtcattgg ccatgacgtc ggcgttggcg acctggctgg tcgccgccga gccctggggc
     4861 cacatgaccc ggtagaagta tgggtcgccc gccgtggtcg gaggggcggc gccgacatcg
     4921 ggcggaccgg gcgcagccga ggcggcggac aggttgtgaa ccgtcccgga actgccgtcg
     4981 gacgcgaccc tgacggcctg gacgaccggc tcggtgccgt caaagcggag ccaggcaaga
     5041 ccggcggtgc cgtcggcgcc gacggcgatc gcgaggtcgc tggcatcctg gccggccggc
     5101 ccgttgaccg accggtcggt gaccgccccg acggtgccgt cggcggcgat cgaaaccgac
     5161 tggatgaaat cggaagtgcc gctctcgttg gtccatccga ccaccgccga gccgtcagcc
     5221 cggctggcga taacgggatt gttggcgttc tggccgaccg gagagagggt gacgatcgga
     5281 ccctgggtgc catcgatctg gactcgggtg ccctggatca ggttgaaacc gcccacgtca
     5341 cgcttccaga cgacccagga agtgccgtcg ctgccaccgg ccaccgtggt ctggctgacg
     5401 ctggtcccgg gcccggaaac cgtggccggc gaagtccagc cggcgctcgc gagcgaaggc
     5461 gaagccatca gcaggcaggc aaaggcgaaa gtcgccagcg cgcccataat cgatccggtt
     5521 tttgcacggc cataccgcaa gcgaaccctc cgtcccagtt gctgtcgggt cagtctagct
     5581 gtaggaacac cggaaacaat caagtgacgc ggtcggtgag gtgagaagcg gcggaagcga
     5641 cgctgctcag tcggcagccg actcggcggc gggaccgtcg gccacgtcag gcttgaccgc
     5701 cggaggctcg ctcgaggggg aatcggtgga accggccccg acaccgacct tctcctgacc
     5761 gtcttccttc ttcttgcgag gcttccgcgg cttggtgatc ttcagcttga gcggcttgtc
     5821 gtcatcgtcg cccgactcgt cgcgggttac cgtgaccagt gacccggcca ccgaatcgcc
     5881 gccgcccgcc ttcagcacct cgtcggccag cggatcctcg atgtagcgct ggatcgcccg
     5941 gcggagcggt cgggcgccca tcgacgggtc ccagcccttg tcggcgagga agtccttggc
     6001 gccgttgtcg agctcgagca tcagctcgtg ctcggccacc tggttcctga cccttgccag
     6061 catcagctcg atgatctcgc gcacttcctc gcgactcagc ttgtggaaga cgatcacttc
     6121 gtcgatccgg ttgaggaact ccggccggaa gaccttccgg agctcgctca tgatgcgacc
     6181 cttcatgtcg tcgtaggtga tgccggtgtc gtcggagacc gtgaagccga tgccctggtt
     6241 gcgggcgatc tcggaagccc cgatgttcga ggtcatgatg atgatcgcgt tgcggaagtc
     6301 cacggtgcgg ccctgcgaat cggtcaggcg gccgtcttcc aggatctgga gcaggatgtt
     6361 gaagacatcc gggtgggcct tctcgatctc gtcgagcagc agcaccgagt acggcttgcg
     6421 gcgcacggcc tcggtcagct ggccgccctc gtcgtagccg acgtagccgg ggggcgagcc
     6481 aaccagacgg ctgaccgcat gcttctccat gtactcggac atgtcgatcc ggaccattga
     6541 ttcctcgtcg ccgaagagga actcggccag ggtcctcgcg agctccgtct tgccgacgcc
     6601 cgatgggccg aggaagacga atgagccggc cggacgcttc gggtccttga tgccggcccg
     6661 cgagcgacgg atcgccttcg agacggcttc gatcgcggct tcctgaccga tcacgcgctt
     6721 gtgcagctcg ctctccatct ggatcagctt cttggtctcg gcctcggtca gcttgaagac
     6781 cgggatgccg gtccacatcg agacgatgtc ggcgatctct tcctcgccga gcaccgggcg
     6841 ctcgccctcc tcgccttcgg cccactcctc ttcgagctgg cgcttgcggg tggtgagctg
     6901 gcgttcggtg tcacgcaggt tggcggcctt ctcgaattcc tgggcctcga tcgcggcttc
     6961 cttctcgcgc cgggcggtct cgatctcttc ttcgagatcg cggtagaccg gtggagcggt
     7021 catcgacttg atccgcatcc gcgaggcggc ttcgtcaatc aggtcgatcg ccttgtccgg
     7081 caggaaacgg tcggagatgt aacggtcggc cagctcggcc gcggcttcca gggcgtcatc
     7141 ggagatctgg accttgtggt gctgctcgta gcgctcgcgc aggccttcga ggatcttcac
     7201 ggtctgttcc ggggtcggct gttcgacccg gatctgctgg aagcggcgct ccagggcaga
     7261 gtcgcgttcg aggtacttgc ggtactcgtc gagggtggtc gcgccgatcg tctggatctc
     7321 gccacgggcc agggccggct tgaggatcga ggccgcgtca atggcgccct cggcggcgcc
     7381 ggcaccgacc agattgtgga tctcgtcgat gaagaggacg atgtcacccc gggaggcgat
     7441 ttccttcatc actttcttga gccgttcctc gaactcgccg cggtacttgg agccggcgac
     7501 cagtgcggcc aggtccaggg tgtagatctg cttgccgcgc agcatctccg ggacctcgcc
     7561 cttgatgatg cgggcggcca gaccctcgac cacggcggtc ttgccgaccc cgggctcacc
     7621 cagcaggacc gggttgttct tggtgcggcg ggaaaggatc tgcatgatcc gctcgatctc
     7681 ggtctcgcga ccgacgaccg gatccagctt gccgtcctcg gccagcttgg tcaggttgcg
     7741 gccgaactgg tcgagcagct tcgaggactt cttgccggaa ccctcgccct ggcttgagcc
     7801 ggaggaggaa ccgctgccac tctggccggg ccggcggccg ccggggccgg aaagcatgcg
     7861 gatgacctcg ttgcggatct tgtccgagtc ggcgtcgaag tcgagcagga tgcgggccgc
     7921 gacaccttcg ttctcacgga ccagtccaag caggatgtgc tcggtgccga tgtagttgtg
     7981 gccgaggctg agcgcctcgc gcagggccag ctccagcacc ttcttcgccc gcggcgtgaa
     8041 ggggatctgg ccggatgtga cttcttcacc ggagccgaca atccggacca cctgtgcccg
     8101 gacgcgctcg acggtgattt caagtgattc caggacccgc gcggcgagac cttcttcctc
     8161 gcgaagcagc ccgagcagga tgtgctcggt gccgatgtag ttgtgcttga gcgttctcgc
     8221 ttcttcctga gcgaggacga ctacctgacg tgcgcgttct gtgaatctct cgaacaagtt
     8281 tcttcttcta cctcaaacct cgatggttgc ctgagtatcg gtgattggtc ggtcggtttt
     8341 tctggtcggt cttgcgagtc ggcaaagtgc cggctgccct atccctgttt cagtcgtgaa
     8401 acaccttggg atctgacctc cggtcttggg ggatcgagcg aataagccag tataacgagg
     8461 cgcctcctcg ggctggatcg gcaatgcggg gttgatcggg gcatcttgcg gcgttatggt
     8521 cggctcatga gggagcacaa gcggggagtt gagttccggt ggctgccgat ccttgccgcg
     8581 gccctggttt ccctcgcgct ggtgatcccg atgctggcac ccacaccttc cggggccgcc
     8641 tacccgtcca gcttccggcg ccatcaggag cagtactggg tctggtacgc cccccggagc
     8701 tggatcgctt cctccggcaa atacgacctc aacatcagct cggcgaccgg caccctctgg
     8761 aacaaatacg gatacagcgc cgtcgtctgt tacaaccccg ccgcctggtt caagtacctg
     8821 cgcaacaact accgggatac cgccggggcc ggtttcggca tctactcgaa gccgctgagc
     8881 cgcacccatt tcacgcggat cggcaggatc aagcagttgc cgaaggccag gtacggcccc
     8941 gcctatttcc ggcagaagct gctctggacc ggccgccgga agggcagcgg ccaggtgatc
     9001 aggggcgaga tgatcatgga catcttcgcg atcgactctt tcggaaactg cgcccagcga
     9061 ttccagaccc gcggcgcccc ggcccgtgga aatgcggcaa gcatcaggct gctgcgcctg
     9121 gttcagtcga cgatcaccca gcgaaacctg tgaggcaacg ctgccggtga ggttaggctt
     9181 gcgaacatga agcgctcgtc cgtcgtcact ttcgcccttg cccttgcgac cgttgccgcg
     9241 ctgctggcgc cggcctcccc cgcctcggcc cgggacgagg tccaccgctg caccaactgg
     9301 tcctggaccg ggccgtcgag ttgggacgcg gtctgcagca agcagggcat cagcatctcc
     9361 tcgccggacg ggcgccgcgg aatcgactgg ggcttcagca acatcttctg tgt
//
LOCUS       DAOQKN010000088         9306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_3537_length_9306_cov_5.364069, whole genome shotgun sequence.
ACCESSION   DAOQKN010000088 DAOQKN010000000
VERSION     DAOQKN010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 9306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9306
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3537_length_9306_cov_5.364069"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PLJ64_07200"
     CDS             <1..242
                     /locus_tag="PLJ64_07200"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HNC93302.1"
                     /translation="YVDFFFDGERGVEITGEVHPGRSAHGSGCTHSSALAAGLALGLG
                     PLEAARKARELASRAVAGSLDGIGSGPGPVNALGL"
     gene            333..2021
                     /gene="ilvD"
                     /locus_tag="PLJ64_07205"
     CDS             333..2021
                     /gene="ilvD"
                     /locus_tag="PLJ64_07205"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNC93303.1"
                     /translation="MTGNDLPANLPRPRSAAVFDGPDRAAARAYMKGIGLSDEDLSKP
                     TIGIANTWTEAMPCNYGLRDLAAYVKEGVKAAGGTPMEFNTVAVSDGITMGTQGMKSS
                     LVSREMVADSIELMARGYQFDAIIALAACDKTIPGAVMGVARIDIPSIVLYGGSVKPG
                     RYKGKDVSILEVFEAVGAHIAGRISDEELTEIENVASPGYGACGGQFTANTMACAFEA
                     LGISPAFSAMVPAEDEQKTVVAEKVGELILQVLADDLKPSRIITRKSLENAIAAVCAS
                     GGSTNGVLHLLAIAREAGVELDIDVFEEVSRRTPLLADLKPGGRYVATDLYRAGGVPL
                     ILRNLKQGGLLHEDEITVTGRSIGEEVANAEEEPGQDVVLPVDAPLKAQGGLAILRGN
                     ICPEGAVVKVAGTERRHHAGPARVFEREEDCFAAVKRGEIEKGDVIVIRNEGPSGGPG
                     MREMLQVTAALVGEGMGEHVALLTDGRFSGATRGLMIGHVAPEAVKGGPIGAIHDGDE
                     ITVDIDARRLEVDLTPEQIDERLKGYSPPTPLYDRGVMAKYAATVSSASMGAVT"
     gene            2072..3280
                     /locus_tag="PLJ64_07210"
     CDS             2072..3280
                     /locus_tag="PLJ64_07210"
                     /inference="COORDINATES: protein motif:HMM:NF022732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HNC93304.1"
                     /translation="MREWSRAIRAIVGIALAGMLIGIASQPAAAAEQKFTWLCKPGRA
                     NDPCQQSLRTETVSKDETLGVRTPRTLTRLRNKVDCFYVYPTVSFQTGPNADLTEDPQ
                     VDAIAGQQASRFTPGCRMFAPIYRQFTVPAILGSEITEQVNDTAYSGVKAAWNEYLGK
                     YNKGRGIVLIGHSQGTGHLKRLIRETFDRNPKLRQRLVSAVLIGGNVTVKKGSRTGGS
                     FRKVPSCARATELGCVIAFSSFLEDPPPADSLFGRVGGALSENVDPATHEVMCVNPAA
                     LDGSKGRLKPLFNTETFPGAYGALLPDLTGYDSPWVSFPDLYTAKCEKADGASWLNVH
                     DVSDESDTRPRVGETLGRTWGTHLAELNLTGGNLASVVATQEKAYLKKLGQARKAGAK
                     KNSKQREGHR"
     gene            3365..4720
                     /locus_tag="PLJ64_07215"
     CDS             3365..4720
                     /locus_tag="PLJ64_07215"
                     /inference="COORDINATES: protein motif:HMM:NF022732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HNC93305.1"
                     /translation="MSRVAGRTWLAVAFLAASFAFIAAVMPSFAKAEDTVWICKPGLA
                     DDLCAGSISTKAGNPAQTIGFDRPDNPPVDCFYVYPTVSAQETPNANLDKDPEVKRVV
                     VQQARMFSRVCDVYAPMYRQDTSPGVYTERTEVAYQSALSGWKDYLQHYNKGRGVILI
                     GHSQGSATLARLIDEEIDPDPKLRKKLVGAILPGGNIYVKKGQLAGGMYENVPACSEV
                     GEYGCLVAYSMFNGTPADNPPFGDVGSGYWAYDMPRPSREQYEVVCTNPAELSGKTLL
                     TSLANMDYLLEPPAGGVETANWNAYPDAVTASCDHEGTKHWLNVAFTEDADPFIKGMI
                     PIVASGNNWHVPEVNVAENNLVEIAQLQTDSYTAEQNRIAKLQAKIKSLQKQLAKAQR
                     NAGLQQKKANKFARQARKAKGKQKEALNRKAGSARRKAASERKRVRNLKQRIAALKKQ
                     I"
     gene            complement(4829..5785)
                     /locus_tag="PLJ64_07220"
     CDS             complement(4829..5785)
                     /locus_tag="PLJ64_07220"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNC93306.1"
                     /translation="MTTEAPDNDGLLTETPPPAPVHDDYGVSDGRWLKIDWSERIRQV
                     TVDSALGQTPVSYVDQGEGAPILLVHGLGGSWRNWLENIPFLADSHRVVALDLPGFGL
                     SPMPTEEISMSAYGDLVLRFADAVGLSPETFLIGHSMGGFISTEAVTSAPERFSRLSL
                     VSAAGISFATISGPRKSLTAAIVKGMIPVAASRAERNLYRKRLRTASFAGVIAHPSMI
                     GNEILWELGTYAALRSPALFAAAFALGGYDNRPSLAEIEIPTQIVWGQQDRLVPVGAA
                     YSYARRIEHAELSLVDDCGHMVQLERPARFNGEISRFAGQTD"
     gene            5838..6965
                     /locus_tag="PLJ64_07225"
     CDS             5838..6965
                     /locus_tag="PLJ64_07225"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNC93307.1"
                     /translation="MADDRTGARRVLVTGLSTYWGGRLASALESNPEIEAIIGVDSRE
                     PTRELTRTEYVKVGVEHRLIEKIVRAARIDTVIDARLMVDSLRGPDGLRPEQIHENNV
                     IGTLNILAACAAGDSPVRKLVFKSSAHWYGCAQDDPAYFTEDMSRRYEPETRVERDVV
                     EAESAVEEFRGQRPKVTVSVLRFANVLGAEVDTSHVQFFALPAVPMIAGFDPRYQFVD
                     EVDVVHALEHVAVRDIPGIFNVAGDGILTLSEVISLLGKRPLPVIPPWGTSLAMAGYR
                     RLGFSLPPEMVTQLRFGRGIDNRAFKATGFHFRFTSRETVLHLRDRLRLDPVAARKEA
                     GYSYEPAVEDFLRHSPHVDRSRKDLGREDAALFDPSPPVQD"
     gene            7115..8110
                     /locus_tag="PLJ64_07230"
     CDS             7115..8110
                     /locus_tag="PLJ64_07230"
                     /inference="COORDINATES: protein motif:HMM:NF023650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNC93308.1"
                     /translation="MLGGAVAAYAYDGAQKDKIADGVTVAGVDLSGMNREEATQALTN
                     QVLAPQRKPVTVTFKKEKFQLPAKQLKIHANVQAAINQAFDESRDGSLPSRVFREITG
                     AEVQASIPVNVGYSQKAVNRFVKKVADGIVKEPVDASVSAGPDSLSVVKAENGYKLRD
                     NKLTDQLTSLLDSGRGSRTIVAKVNVTKPAVTTAEVAEKYPTYLTLDRSNFTLTLWKN
                     LEKVKSYTVAVGQVGLETPAGLYSIQDKQVDPVWTVPNSAWAGDMAGQVVPGGVPENP
                     LKARWMGIFDGAGIHGTDETYSLGSAASHGCVRMSVPDVIDLYDRVDVGTPIYIG"
     gene            8082..9002
                     /locus_tag="PLJ64_07235"
     CDS             8082..9002
                     /locus_tag="PLJ64_07235"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HNC93309.1"
                     /translation="MSALPSTSASCPAWGEALELFRRDLLAAGTAPRTLNAYEKDLGQ
                     LRAWADGSGLGVDKVTHKDLRRYAASLSAAGRAPATVARKLAAIRGFYGFLLRTGRVG
                     QNPADLVSAPRRKKKLPTVLTMEQMSSMLDAVPVETPLDIRDRAMFELTYSCGLRSEE
                     VLTLGLDSIDFDDEVIRVLGKGSKHRLVPVGEPARQAIELYLARARPKLATDPAERTL
                     FLSRNGRPLSASDLSRRLAVRMRDAAAAGGVSPHALRHSFATHLLEGGADLRSIQELL
                     GHASISTTQVYTHLDAARLRDAYTGSHPRA"
     gene            9057..>9306
                     /locus_tag="PLJ64_07240"
     CDS             9057..>9306
                     /locus_tag="PLJ64_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase-like protein"
                     /protein_id="HNC93310.1"
                     /translation="MKLIVNVDGGARGNPGPAAIGIVVRDGEGNVLQDLGETIGEATN
                     NVAEYRALIRGIGLARELGATELQIHGDSELVVKQMLGQ"
BASE COUNT         1736 a         3032 c         3071 g         1467 t
ORIGIN      
        1 agtacgtcga tttcttcttc gacggggaac gcggggtgga gatcaccggc gaggttcatc
       61 ccggccgctc cgcccatggc tccggttgca cccattcctc ggccctcgcc gccggcctcg
      121 cgctggggct cggtcccctc gaagccgctc gcaaggcccg cgaactcgcc tcaagggcgg
      181 tggccggaag cctggacggg atcggttcgg gtcccggccc ggtcaacgca ctcggcctgt
      241 gaaacaacca gcctgaggag cctgggttga agttatccgc acatgtgacg aataacttca
      301 aggcaggtgg aaggcggctc taggatggga acatgaccgg gaacgacctg cctgcgaatc
      361 tgccccgtcc tcgatcggct gcggtcttcg atggtcccga ccgggcggcc gcccgggcct
      421 acatgaaggg catcggactg agtgacgaag acctcagcaa gccgaccatc ggaatcgcca
      481 acacatggac cgaggcgatg ccctgcaact acggcctgag ggacctcgcg gcctatgtca
      541 aagaaggtgt gaaggcggcc gggggcaccc cgatggagtt caacacggtc gccgtctccg
      601 acggcatcac gatgggcacc cagggaatga agagttccct ggtcagtcgc gagatggtcg
      661 ccgactcgat cgagctgatg gcccgcggct accagttcga cgcgatcatc gcgctcgccg
      721 cctgcgacaa gacgatcccc ggcgccgtga tgggtgtggc ccggatcgac atcccctcga
      781 tcgtcctcta cggcggctcg gtcaagcccg gcagatacaa gggcaaggac gtcagcatcc
      841 tcgaggtctt cgaggcggtc ggtgcccaca tcgccggcag gatcagcgac gaggaactga
      901 ccgagatcga gaacgtggcc agccccggct acggcgcctg cggcggccaa ttcacggcca
      961 acacgatggc ctgcgccttc gaggcactgg ggatctcgcc ggccttctcg gcgatggtcc
     1021 cggccgagga cgaacagaag accgtggtcg ccgaaaaagt cggggagctg atcctgcagg
     1081 tcctggccga tgatctgaag ccgagccgga tcatcacgcg caagtcgctg gaaaacgcga
     1141 tcgccgcggt ctgcgcttcc ggcgggtcga ccaacggggt tcttcatctg ctggcgatcg
     1201 cccgtgaagc cggggtggag ctggacatcg acgtcttcga ggaggtctcc cgccgcaccc
     1261 ccctgctggc cgacctgaag cccggcgggc ggtacgtcgc gaccgacctc taccgggccg
     1321 gcggggtgcc gctgatcctc agaaacctca agcagggcgg cctgctgcat gaagacgaga
     1381 tcacggtgac cgggcgcagc atcggcgagg aggtcgccaa cgccgaagag gagccgggcc
     1441 aggacgtggt tttgccagtc gatgccccgc tcaaggccca gggagggctg gccatcctgc
     1501 gcggcaacat ctgccccgaa ggggcggtgg tcaaggtggc gggaacagaa cgccgccatc
     1561 acgccggtcc cgcccgggtc ttcgaacgcg aggaggactg cttcgccgcg gtcaaacggg
     1621 gggagatcga gaagggcgac gtgatcgtga tcagaaatga agggccatcg ggcggaccgg
     1681 ggatgcgcga gatgctccag gtcaccgctg ccctggtcgg cgaggggatg ggcgagcacg
     1741 tcgccctgct gaccgacgga cgcttctccg gggcaacccg gggcctgatg atcggccatg
     1801 tcgcaccgga agccgtgaag ggcgggccga tcggggcgat ccacgacggc gacgagatca
     1861 ctgtcgacat cgacgcgcgg cgcctcgaag tggacctcac ccctgaacag atcgacgagc
     1921 ggctgaaggg ctactcaccg ccgacgcccc tctatgatcg cggggtcatg gcgaagtacg
     1981 ctgcgaccgt gagttccgcc tcgatgggcg cggtcacgta ggagatgaag acgagggggc
     2041 ctgggggctc ccgggaggag aggacagttt catgagggag tggagcaggg ccatccgggc
     2101 gatagtcggc atcgcattgg cagggatgtt gatcggcatc gcttcgcaac ccgccgcggc
     2161 ggcggagcag aagttcacct ggctctgcaa accgggaagg gcgaacgatc cctgccagca
     2221 gagcctccgc acggagacgg tctccaagga cgaaaccctc ggagttcgca cgccccggac
     2281 cctgacccgc ctgaggaaca aggtcgactg cttctatgtc tacccgacgg tgagcttcca
     2341 gaccggtccc aacgccgacc tcaccgagga cccgcaggtt gacgcgatcg ccggacagca
     2401 ggcgagccgg ttcacgcccg gttgccgcat gttcgccccg atctaccgcc agttcacggt
     2461 gccggcaatc ctcggttccg agatcaccga gcaggtgaat gacacggcct actccggggt
     2521 caaggccgcc tggaacgagt atctggggaa atacaacaag ggccggggca tcgtcctgat
     2581 cggccactcc cagggcaccg gtcacctcaa gcggttgatc agggaaacct ttgaccgcaa
     2641 cccgaagctc cgccagcgcc tggtctccgc ggtcctgatc ggcggcaacg tcaccgtgaa
     2701 gaagggaagc cgcaccggtg gcagcttccg caaggtgccg tcatgcgcga gggcaaccga
     2761 actcggttgc gtgatcgcct tctcgagttt cctggaggat ccgccgccgg ccgattccct
     2821 ctttggcagg gtcggagggg ccctctcgga gaacgtcgac ccggccaccc acgaggtgat
     2881 gtgcgtgaac ccggccgcgc tcgacgggtc gaagggcagg ctcaaacccc tgttcaacac
     2941 cgagaccttc cccggcgcct acggggcatt gctgcccgac ctgaccggct acgacagccc
     3001 ctgggtcagt ttccccgacc tctacacggc gaagtgcgag aaggccgatg gtgccagctg
     3061 gctgaacgtt cacgatgtct ccgacgagtc agacacccgc ccccgggtcg gggagaccct
     3121 gggccggacc tggggcacgc acctggccga gttgaacctg accggcggca atctggccag
     3181 tgtcgttgcg acccaggaga aggcctacct gaagaagctc gggcaggccc gcaaggccgg
     3241 ggcgaagaag aattcgaaac agagagaggg tcatcgctag cggccggacc gaaccggcca
     3301 gcctgcagcg gtgttatcga tgatgcatag tccagaagtc aatttccccg ggaggagtct
     3361 gatcgtgtcg agagttgctg gtcgtacctg gcttgccgtg gcctttttgg ccgcgtcatt
     3421 cgccttcatt gccgcagtga tgccgtcttt cgcgaaagcc gaagacacag tctggatctg
     3481 caagcccgga ctggccgatg atctctgcgc cggctccatc tcgaccaagg ccggcaatcc
     3541 ggcccagacg atcggcttcg atcgtcccga caatcctccg gtggactgct tctacgtcta
     3601 cccgacggtc agcgcgcagg agactcccaa cgcgaacctg gacaaggacc ccgaagtcaa
     3661 gcgggtcgtg gtgcagcagg cccgcatgtt ctcccgggtc tgcgacgtct acgcgccgat
     3721 gtaccggcag gacacttcgc cgggcgtcta caccgagcgg acagaggtcg cctaccagag
     3781 cgctctttcc ggctggaagg attacctcca gcactacaac aagggccgcg gcgtgatcct
     3841 gatcggccac tcgcagggct cggcgaccct ggctcgcctg atcgacgagg agatcgaccc
     3901 cgatccgaag ctgcgcaaga agctggtcgg agcgatcctg cccggcggca acatctacgt
     3961 gaagaagggc cagctggccg gtggcatgta cgagaacgtg ccggcctgct cggaagtcgg
     4021 cgagtacggc tgcctggtcg cctactcgat gttcaacggc accccggccg acaacccgcc
     4081 tttcggcgat gtcggctccg gctactgggc ctatgacatg ccccggcctt cccgcgaaca
     4141 gtacgaggtg gtctgcacta atccggctga attgagcggc aagaccctgc tcacctcgct
     4201 ggccaacatg gactacctgc tcgagccgcc ggccggcggc gtcgagacgg ccaactggaa
     4261 cgcctacccg gatgcggtca ccgcctcctg tgaccacgaa ggcaccaaac actggctgaa
     4321 cgtcgccttc accgaagacg ccgatccctt catcaaggga atgatcccga tcgtcgccag
     4381 cggcaacaac tggcatgtgc cggaagtcaa cgtcgccgag aacaacctgg tcgagatcgc
     4441 ccagctccag accgacagct acacggccga gcagaaccgg atcgcgaagc tacaggccaa
     4501 gatcaagtcg ctccagaagc agctggccaa ggcccagagg aacgccgggc ttcagcagaa
     4561 gaaggcgaac aagttcgcca ggcaggcccg caaggcgaag ggcaagcaga aagaggccct
     4621 gaaccgcaag gccggctcag cccgccggaa ggcggcttcc gagcgcaagc gggtcaggaa
     4681 cctcaagcag cggatcgccg ctctgaagaa gcagatctag gcggattcgg cgggatcggc
     4741 cggtttgccc gaggcgaacc ggcctgagaa acggtgctca gatcagtcgg tttgccctgc
     4801 aaaccggccc tgagaaacgg tgctcagatc agtcggtttg ccctgcaaac cgactgatct
     4861 cgccgttgaa gcgggcaggc cgctcgagct ggaccatgtg gccgcaatcg tcgaccagcg
     4921 aaagttccgc gtgctcgatc cggcgtgcgt acgaataggc ggcgccgacc gggacgagcc
     4981 ggtcctgctg gccccagacg atctgggtcg ggatttcgat ctcggcgagg ctgggccggt
     5041 tgtcgtatcc accgagggcg aaggcggcgg cgaaaagcgc cggcgagcga agtgcggcgt
     5101 aggtcccgag ctcccagagg atttcgttgc cgatcatgct cgggtgggcg atgaccccgg
     5161 cgaatgacgc ggttctgagg cgcttgcggt agaggttccg ttcggccctg ctcgcggcaa
     5221 ccgggatcat ccccttgacg atcgccgcgg tcagggactt gcgggggccc gaaatggtgg
     5281 cgaagctgat cccggccgcc gagaccaggc tcaggcggga gaagcgttca ggtgcgctgg
     5341 taaccgcttc ggtcgagatg aagccgccca tcgaatgacc gatcaggaag gtctcgggag
     5401 aaagcccgac ggcatcggcg aagcggagga cgagatcgcc gtaagcggac atcgaaatct
     5461 cttccgtcgg catcgggctg aggccgaagc ccggcagatc cagggcgacg acgcggtggc
     5521 tgtcggccag gaaggggatg ttctccagcc agttgcgcca gctgcccccg agtccgtgga
     5581 ccagaaggat cggcgcgccc tcgccctgat ccacgtagct gaccggcgtc tggccgagcg
     5641 cggagtcgac ggtcacctgc cggatccgtt cggaccagtc gatcttcagc cagcgtccgt
     5701 ccgagacgcc atagtcgtcg tggaccgggg ccgggggagg agtctcggtc agcaggccgt
     5761 cattgtcggg tgcctcggtc gtcacccgac cgactttata tgcccgccga ccccgcctcg
     5821 cctgccacaa tggccgggtg gcggatgacc gcacaggcgc acgccgggtc ctggtgacgg
     5881 gactctcgac ctactggggc gggaggctgg cctctgccct ggaatcaaat cccgaaatcg
     5941 aggcgatcat cggggtcgac tcccgcgagc cgacccggga actgacccgg accgagtacg
     6001 tgaaagtcgg ggtggaacac cgcctgatcg agaagatagt ccgggcagcg cggatcgaca
     6061 ccgttatcga cgcccgcctg atggtcgatt cgctgcgcgg tccggatggc ctccggcccg
     6121 agcagattca cgagaacaac gtgatcggca cgctcaacat cctcgccgcc tgcgcggccg
     6181 gtgattctcc ggtcaggaag ctggtgttca agagctccgc ccactggtac ggctgcgccc
     6241 aggacgaccc ggcctatttc accgaggaca tgtcccgccg gtacgagccg gaaaccaggg
     6301 tcgagcggga cgtggtcgag gccgagtcgg cggtcgagga gtttcgcggg cagcgaccga
     6361 aggtcacggt ctcggtgttg cgcttcgcga acgtgctcgg agccgaggtc gacacttcac
     6421 acgtccagtt cttcgccctg ccggcggtgc cgatgatcgc cgggttcgac ccgcgctacc
     6481 agttcgtcga tgaagtcgac gtggttcatg cgctcgaaca cgtggcggtg cgggacatcc
     6541 ccgggatctt caacgtggcc ggcgacggca tcctgaccct ttccgaggtg atctcgctgc
     6601 tcggcaaacg gcctctgccg gtgattccgc cctggggcac ttcgctggcg atggccggtt
     6661 accgccggct cggcttctcc ctgcccccgg agatggtcac gcagcttcga ttcggacggg
     6721 gaatcgacaa tcgcgccttc aaggccacgg gcttccattt ccgcttcact tccagagaga
     6781 ccgtgctcca tctgcgggac cggttgcgtc tcgatccggt agctgcccgg aaggaagccg
     6841 gctacagcta cgagccggcg gtcgaggatt tcctgcgcca cagcccccat gtggaccgct
     6901 cccgcaagga tctgggccgc gaagacgcgg cgcttttcga cccctcgccg ccagtccagg
     6961 actgaatttc cgctgcaaag gctgtttccg cccggttaga gggttttgcc cgcccgccgt
     7021 ggtatctaca attcggagag gaattaacta gccagagagc gggattgaag tggggcggaa
     7081 gactcaggta gccatagtcg caatcgttgt cctgttgctg ggcggcgcag tcgctgccta
     7141 cgcatatgac ggcgcacaga aggacaagat cgccgacggc gtgaccgtcg ccggggtcga
     7201 cctcagcggc atgaaccgtg aggaagccac ccaggcgctg accaatcagg tgctggcgcc
     7261 gcaacgcaag ccggtcaccg tcaccttcaa gaaggaaaag ttccagcttc ccgccaagca
     7321 gctgaagatt cacgccaacg ttcaggcggc gatcaatcag gccttcgacg agagccggga
     7381 cggttcgctg ccctcaaggg tcttccgcga gataaccggc gccgaggttc aggccagcat
     7441 tccggtcaac gtcggctatt cgcagaaggc ggtgaacagg ttcgtcaaga aggtcgccga
     7501 cgggatcgtc aaggaaccgg tcgacgcttc ggtttcggcc ggcccggatt cgctttcggt
     7561 ggtcaaggcc gagaacggct acaagctgcg ggacaacaaa ctcaccgatc agctgaccag
     7621 cctgcttgac agcggtcgtg gcagccggac gatcgtggcc aaggtcaacg tgaccaagcc
     7681 ggccgtcacc accgccgaag tcgccgagaa gtatccgacc tatctgaccc tggaccggag
     7741 caacttcacc ctgaccctct ggaagaacct cgaaaaggtc aagagctaca cggtcgcggt
     7801 cggccaggtc ggcctcgaga ctcccgccgg gctctactca atccaggaca aacaggtcga
     7861 tcccgtctgg acggtgccga actcggcctg ggccggagac atggccggtc aggtcgttcc
     7921 cggcggggtt cccgaaaacc ccctgaaggc tcgatggatg gggattttcg atggcgccgg
     7981 catccacggc acggatgaaa cctactcgct cggttcggcc gcctcgcatg gttgcgtgcg
     8041 gatgtcggtc ccggacgtga tcgaccttta cgaccgggtc gatgtcggca ctcccatcta
     8101 catcggctag ctgccccgcc tggggcgagg ctctcgagct cttccggcgc gatcttctcg
     8161 cggcgggtac ggcaccgcga accctgaacg cctacgaaaa ggacctcggc cagctgcggg
     8221 cctgggccga tgggtcaggc ctgggggtag acaaggtcac ccacaaggat cttcgccgct
     8281 acgccgcctc gctttcggcg gcgggccgcg ccccggccac ggtggctcgc aaactggccg
     8341 cgatccgcgg cttttacggc ttcctcctca ggaccggaag ggttggccag aacccggccg
     8401 acctggtttc cgcaccccgc cggaagaaga agctgccgac cgttctgacg atggaacaga
     8461 tgtcctcgat gctggacgcg gtaccggtcg aaaccccgct tgacatcagg gaccgggcga
     8521 tgttcgaact cacctactcc tgcggtctgc gaagcgaaga ggtactgacc ctgggcctcg
     8581 actcgatcga tttcgacgac gaagtgatcc gggtgctcgg caaggggtcc aagcaccggc
     8641 tggtcccggt cggcgagccg gcccggcagg cgatcgagct ctacctcgcc agggcccggc
     8701 cgaaactggc caccgacccg gccgagagga ccctgttcct ttcgcgaaac ggccgacccc
     8761 tttcggcctc cgacctttcc cgtcgcctgg cggtccgcat gcgggatgcc gccgcggccg
     8821 gcggggtttc gccccatgcc ctcaggcatt ccttcgccac ccacctgctc gaaggcgggg
     8881 cggacctgcg ctcgattcag gaactgctcg gccacgcctc gatctcgacc acacaggtct
     8941 acacccacct cgacgcggcc cgcctgcggg acgcgtacac cggcagccat ccccgcgcct
     9001 gaaggggcgc ttcagccggg agtcgaccgc ccggcgcagg caatagattc cctgccatga
     9061 agctgatcgt caacgtggac ggcggcgccc ggggcaatcc gggcccggct gcgatcggaa
     9121 tagtcgttcg ggacggtgag ggcaacgtgc tccaggatct cggcgagacg atcggcgaag
     9181 ccacaaacaa cgtggccgag tacagggccc tgatcagggg catcggcctg gcccgggaac
     9241 tcggcgcgac cgaacttcag atccacggag attcggaact ggtcgtcaag cagatgctcg
     9301 gtcagt
//
LOCUS       DAOQKN010000089         9290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_3549_length_9290_cov_5.762534, whole genome shotgun sequence.
ACCESSION   DAOQKN010000089 DAOQKN010000000
VERSION     DAOQKN010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 9290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9290
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3549_length_9290_cov_5.762534"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1639
                     /locus_tag="PLJ64_07245"
     CDS             <1..1639
                     /locus_tag="PLJ64_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93311.1"
                     /translation="IAQSGDTWSLHLLAPQFLSWFYPQNSEVIHSLGMMAFGTDLLSI
                     FLNLGWFAACLLAAWCIGRPYGAAPISMAGVALILGSTAMADQAGEARNDIVGTFFLL
                     AGLAVMVNAAAGGRKITLGPTLVVALAAGLAMGTKVNFIPAALVLLAGTVVLAEPSRR
                     LRNGLAGSGALLLGGGYWYLRNLTHAGNPLPWINHIGPLSLPGPEQDVGGRDAGSVWG
                     YLTDTDVVRHWFLPGFGDGFGSGWILLGVLAITGLVLCLDRNSGPARRTGAAVGLALV
                     LAWLVAPTSASGPAGEPNGFVSGLRYLAPALAVCLALLGSGVGQRGGRARWLVAGLLL
                     ILAPVTIAFGDGWDAKEWLVAIVAATVFWVVCLIALGVWRVRSKQHRLSTRSSRKLFG
                     WTAGATIAFLVLCGFGVQRYYFNHRYASPDFTIPALSESYVWANGQSGVKIGTNATRQ
                     YPLWGEDLDNEVQFIGVKQPHAGFVEAGNCRDFIAAANQGDYEYLVLTLDREGQKLLV
                     PRELTWVSRDPAVKNVIDADGGAVLALDGPLSPRSCR"
     gene            1668..3461
                     /gene="lepA"
                     /locus_tag="PLJ64_07250"
     CDS             1668..3461
                     /gene="lepA"
                     /locus_tag="PLJ64_07250"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006303521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNC93312.1"
                     /translation="MELIRNFSIIAHIDHGKSTLADRILELTKAVDPLKMQAQVLDSM
                     DLERERGITIKAQAVRVEFMASDGVTYHLHLIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAAQGVEAQTVANTYLAIENGLELIPVLNKVDLPGAEPERVAAEVSDLIGVDPDDILR
                     ISAKTGEGVIEVLEAVVDRIPPPEGDPDAPARALIFDSEFDQYRGVIAYVRLMDGRFR
                     KHGKVLAMQNGTEAELDDIGFFRPVMTPVDGIDTGEVGYVITGIKDVSKLRVGDTLTN
                     LENPAAEALAGYREVKPMVFCGLFPIDTDRYEDLRDALDKMGLNDAALSWEPETSEAL
                     GFGFRCGFLGLLHMEIVRERLEREYGLELMATSPTVRYEVHTTNGEMTEISSPNQMPD
                     PGSIERIEEPYIRATVITPTEYVGPVMELCQSRRGTHVDMDYLSPTRVQIRYDLPLGE
                     IVLDFFDVLKSSTRGYASLDYEPIGLRPSDLVKVDVLLAGDRVDALSIITHKEFAYNQ
                     GKDLVARLRKTIPRQQFDIPVQAAIGANILARETVKAVRKDVTAKLYGGDVTRKNKLL
                     DKQKKGKKRMKNVGRVEVPQEAFLAVLELDE"
     gene            3586..4032
                     /locus_tag="PLJ64_07255"
     CDS             3586..4032
                     /locus_tag="PLJ64_07255"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HNC93313.1"
                     /translation="MALKTDAVGKSWPAYEYEVGREKIREYAQVIGLDNPVHFDAEAA
                     RAAGYRGIVAPPMFVVVYAAGAMGPVLFDPDVEMNFAAMVHGGQEFEWGEPVISGDLI
                     TTTTTCRDIYPKDGKGFYVFETESKNQNGDLVCKATWTNIVRGIEE"
     gene            4029..4424
                     /locus_tag="PLJ64_07260"
     CDS             4029..4424
                     /locus_tag="PLJ64_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HNC93314.1"
                     /translation="MSAKEKGEKIAGLKVTPDKFLPHRYAGASGDFNPIHIDKEFAQM
                     VGLPSTILHGLYVMGLVAKAATEEAGGDPRALKRLSVQFRGMSMPETEIEVSGEVIEA
                     DGADVTFDLIAAQGENQVIRNATADVTYS"
     gene            4470..5495
                     /gene="hrcA"
                     /locus_tag="PLJ64_07265"
     CDS             4470..5495
                     /gene="hrcA"
                     /locus_tag="PLJ64_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HNC93315.1"
                     /translation="MLSERQELILGLVVDAYRETGRPVGSKAIAGDSRLEWGASTVRA
                     ELAALEAGGYLTHPHTSAGRVPTDLGYRQYVESLKSRAVTRGAPAATLDLSDLRREVD
                     GAMQEATSALAQITDLLALVVAPPLGTARIHRVEALLLQPEVVMVIVITSTGDVTRRA
                     FTFASPVDPGLVEWASSYLNESLSGIDLGARRIASKLADPALGPLEANFIEQLAPALV
                     DLEQGLEGTLYVDGASRLLDQRRAADLPRIDRLMTTLEEKATVLRMMRSAIDERSVFL
                     WIGAENPQPELQDVTVIGANYGLGHRNLGSVGVVGPTRMDYEKAIASVSEAARELSRY
                     FEFVYDA"
     gene            5492..6607
                     /gene="dnaJ"
                     /locus_tag="PLJ64_07270"
     CDS             5492..6607
                     /gene="dnaJ"
                     /locus_tag="PLJ64_07270"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNC93316.1"
                     /translation="MSEKRDYYEILGVSREATDAEIKKAFRKVARELHPDVNAHDPEA
                     EEKFKEAAEAYEVLSDAERRGTYDQFGHDGLRSGGYQSQAQGFGSIDDLFSAFFGGGF
                     GASRGPAPGADVGVRTEIELGDVLDGTTREVEFDAVTSCEHCHGNGAEPGTPIRTCDR
                     CGGSGELRSVANTAFGQMVRATPCDRCHGDGRIPEEPCKVCSGLGRKHTAKKHRIDIP
                     AGIESGQRIRISGAGHAGETGAPPGDLYVEVQVAEREGMVRDGQDLITVVPVDATAAM
                     IGDTLEVETLDGEDEIELAPGTQPGAETRLKGKGLPQLGSSRRRGDHRFIFNVIIPAN
                     LSEQQQKLAADLDGTLTDENREPKESGIFSRMRRAFG"
     gene            6604..7452
                     /locus_tag="PLJ64_07275"
     CDS             6604..7452
                     /locus_tag="PLJ64_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNC93317.1"
                     /translation="MIRLAVRCQPEQAELVLAELTVLAPGGVEEVAADGYVEYAIYGA
                     EGELPDLGEEEKVVIAGGTVEVEATEVADDWADRWRTFHKPLLVGDPDRGPAVWIRPP
                     WEPERADLAEVVVDPGQAFGTGAHPTTRLCIEALIEIAGRGDPFGPLTDLGCGSGVLA
                     IAAGKLGWRPLEGCDNEVAAVEAARENARVNGVEAGFDRIDLKSGLPALAPTTVANLT
                     APLLEIVANGLSAENLPETLICSGLLTTEYDRVLAAFGPHGLVETGRGAIGDWGSLVF
                     AREGSR"
     gene            7449..8264
                     /gene="murI"
                     /locus_tag="PLJ64_07280"
     CDS             7449..8264
                     /gene="murI"
                     /locus_tag="PLJ64_07280"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNC93318.1"
                     /translation="MTRSTLPIGVFDSGVGGLTVLHECLISLPAEDFLYLGDSGHFPY
                     GTKSAEELRDRAQVIATGLLERGVKMLVIACNSATAAAGDEIRQLGAEAGVPVVTVVG
                     PEAEIAATISGNGKVGVLATPATVASGAYRRALDSLGRPLTVTEVAAPDLAPIIQQGP
                     TFDQGVLDTVRSYCEPLREAGVDTLILGCTHYPLVAPMLQRMLGRDVSLVTAGHAIAA
                     AIQRELASRDELTTDRIEGDYRFLVTGDVDSFVEAGTRFLQLPLAGRAEKVEL"
     gene            8261..8623
                     /locus_tag="PLJ64_07285"
     CDS             8261..8623
                     /locus_tag="PLJ64_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93319.1"
                     /translation="MNQDDQQNSRNTATARFAGWLAVGVLFMVGCIAPISFFDWIPAA
                     LLAGFLIWRGVRSPRYFFAFAAGIGITLVGLGIVNGTYRIMLLYGSVVIAGGIVFYAA
                     FGPRTSHAEPPGGKGSQS"
     gene            8681..9112
                     /locus_tag="PLJ64_07290"
     CDS             8681..9112
                     /locus_tag="PLJ64_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HNC93320.1"
                     /translation="MSAADQIREDAQAALKDGDRKRASALRMIQDVLQQENKLGKGDE
                     ISALQREHKKRLEAAKAFADAGRYEQAEDERFEAELIEAYLPAQLSDEELADLARLAI
                     AETGATEQKHMGQVMGLLKERVAGRADGKRVSTEVRSQLNG"
BASE COUNT         1605 a         3139 c         3073 g         1473 t
ORIGIN      
        1 catcgcccag agcggggaca cctggtcgct ccatctgctc gccccgcagt tcctcagctg
       61 gttttacccc cagaactcgg aagtcatcca ctcgctcggg atgatggcct tcggcaccga
      121 cctgctctcg atctttctca acctgggctg gttcgccgcc tgcctgctcg cggcctggtg
      181 catcgggcgg ccctacggcg cagccccgat ctcgatggcg ggggttgcgc tgatcctcgg
      241 ttcgaccgct atggccgacc aggccgggga ggcgagaaac gacatcgtcg gaaccttctt
      301 cctgctggcc ggtctggcgg taatggtcaa cgccgcggcc ggagggcgca agatcaccct
      361 cggtccgacc ctcgtggtcg ctctggcggc gggcctcgcg atgggcacca aggtcaactt
      421 cattccggcc gccctggtcc tgctcgccgg aacggtggtt ctggccgagc cgtcgcgccg
      481 cctgagaaac ggccttgcgg gcagcggggc gctgctgctc ggcgggggct actggtacct
      541 gcgcaacctg actcacgccg gcaatccgct gccctggatc aaccacatcg gcccgctttc
      601 gctgcccggg cccgaacagg acgtgggcgg gcgcgacgcc ggctcggtct gggggtatct
      661 gaccgacacc gatgtggtcc gccactggtt cctgcccggc ttcggtgacg gcttcggctc
      721 cggctggatt ctgctcggag tgctggcgat caccggactg gtgctctgcc tcgatcggaa
      781 ttccggcccg gcccgccgca ccggggccgc ggtcggcctc gccctggtcc tggcctggct
      841 ggtcgccccg acctccgcct ccggccccgc cggggaaccg aacggattcg tctccggact
      901 ccggtacctg gccccggcgc tggcggtctg cctagcgctg ctcggcagcg gcgtcggtca
      961 gcgcggcggc cgggcccgct ggctcgtggc cggtctgctg ctcatcctgg ccccggtgac
     1021 gatcgccttc ggtgatggct gggacgccaa ggaatggctg gttgcgatcg tggcggcgac
     1081 ggtcttctgg gttgtctgtc tgatcgccct cggggtctgg cgggtccggt cgaaacagca
     1141 cagactctcg acccgctctt cccggaaact tttcggctgg accgccggcg cgacgatcgc
     1201 attcctcgtc ctctgcgggt tcggggtcca gcgctattac ttcaaccacc gctacgcctc
     1261 gccggatttc acgatcccgg ccctctccga gtcatatgtc tgggcgaacg gccagagcgg
     1321 agtgaagatc ggcaccaacg caacccgcca gtacccgctt tggggcgagg acctcgacaa
     1381 cgaggtccag ttcatcggcg tcaagcagcc ccacgccggc ttcgtcgaag ccgggaactg
     1441 tcgcgacttc atcgccgccg ccaaccaggg cgattacgag tacctggtcc tcaccctcga
     1501 ccgcgaaggc cagaagctgc tcgtgccccg ggagctgacc tgggtttctc gggatcccgc
     1561 ggtcaaaaac gtgatcgacg ccgacggagg ggcggtactc gccctcgacg ggccactctc
     1621 gccgcgatcc tgccgatagt cccctgcggc atactgctct gtccctgatg gaactgattc
     1681 gcaacttctc gatcatcgcc cacatcgacc atggcaagtc gaccctggcc gaccgcatcc
     1741 tcgaactgac caaggccgtg gacccgctga aaatgcaggc ccaggttctg gactcgatgg
     1801 atctcgagcg tgagcgcgga ataaccatca aggcccaggc ggtgcgggtc gagttcatgg
     1861 cctccgacgg cgtcacctac cacctgcacc tgatcgacac tcccggccac gtcgacttct
     1921 catacgaggt ctcgcgcagc ctcgccgcct gcgagggtgc gctcctggtc gtcgatgctg
     1981 cccagggggt cgaggcccag accgtcgcca acacctacct ggcgatcgag aacgggctcg
     2041 agctcattcc cgtcctgaac aaggttgacc tgcccggcgc cgagccggaa cgggtcgcgg
     2101 cagaagtctc ggacctgatc ggcgtcgatc ccgacgacat ccttcgcatc tccgccaaga
     2161 ccggcgaggg cgtgatcgaa gtgctcgaag cggtggtcga ccgcatcccg ccgccggaag
     2221 gcgaccccga tgccccggcc cgggccctga tcttcgattc cgaattcgac cagtaccgcg
     2281 gcgtgatcgc ctacgtgcgg ttgatggacg ggcgcttccg caagcacggg aaggtgctgg
     2341 cgatgcagaa cggcaccgag gccgaactgg atgacatcgg cttctttcgc ccggtcatga
     2401 ccccggtcga cgggatcgac accggcgagg tcggctacgt gatcaccggc atcaaggacg
     2461 tttcgaagct gcgggtcggt gacacgctga ccaacctcga gaaccccgca gccgaggcgc
     2521 tggccggcta ccgcgaggtc aagccgatgg tcttctgcgg ccttttcccg atcgacaccg
     2581 accgctacga ggacctgcgg gacgcactcg acaagatggg cctgaacgac gccgcccttt
     2641 cctgggagcc cgagacttcc gaggcgctgg gattcggctt ccgctgcggg ttcctcgggc
     2701 tgcttcacat ggagatcgtg cgcgagaggc tggagcgcga atacggcctc gagctgatgg
     2761 cgaccagccc gaccgtgcgc tacgaggtcc acacgaccaa cggcgagatg accgagatct
     2821 ccagtccgaa ccagatgccc gaccccggtt cgatcgaacg gatcgaagag ccctacatcc
     2881 gggccaccgt catcaccccg accgagtacg tgggcccggt gatggagctc tgccagtcgc
     2941 ggcgcggtac ccatgtggac atggactacc tcagcccgac ccgggtccag atccgttacg
     3001 acctgccgct cggcgagatc gtgctcgact tcttcgacgt gctcaagtcc tcgacccggg
     3061 gatacgcctc gctcgactac gaaccgatcg ggctccgccc ctcggacctg gtcaaggttg
     3121 acgtgctgct ggccggcgac cgggtcgacg cgctctcgat catcacccac aaggaattcg
     3181 cctacaacca gggcaaggac ctggtcgcga ggctccgcaa gacgatcccc cgccagcagt
     3241 tcgacatccc ggtccaggcc gcgatcgggg cgaacatcct cgcccgcgaa acagtcaagg
     3301 cggtccgcaa ggacgtcacc gcgaagctct acggcggtga cgtaacccgg aagaacaagc
     3361 tgctcgacaa gcagaagaag ggaaagaagc ggatgaagaa cgtcggccgg gtcgaagtcc
     3421 cccaggaagc cttcctcgca gtcctcgagc tcgacgagta acccgccgcg tcccgggcgg
     3481 cgtcccggcc cgcccccccg ggcggccccg cgagatccca ctttgtgccg cataccggca
     3541 caaaccgggc actcggtgaa cccgggccgg gtgtaagttt ccggcatggc gctgaagacc
     3601 gatgccgttg gcaagagctg gccggcctac gagtacgagg taggccggga aaagatccgc
     3661 gaatacgcgc aggtgatcgg gctcgacaac ccggtccact tcgacgccga agctgccagg
     3721 gcggcgggat accgcgggat agtggccccg ccgatgttcg tcgtcgtgta tgcggccggg
     3781 gcgatgggtc cggtgctatt cgaccccgac gtggagatga acttcgccgc gatggtccac
     3841 ggcggccagg aattcgaatg gggggaaccg gtgatctccg gcgacctgat cacaaccacg
     3901 accacctgca gggacatcta cccgaaggac ggcaagggtt tctacgtctt cgagaccgag
     3961 tcgaaaaacc agaacggtga cctcgtctgc aaggccacct ggaccaacat cgtcaggggg
     4021 attgaagaat gagcgcgaag gaaaagggcg agaagatcgc cggcctcaag gtcactccgg
     4081 acaagtttct gccgcaccgc tacgccgggg catccgggga cttcaacccg atccacatcg
     4141 acaaggaatt cgcccagatg gtcggcctgc cctcgaccat cctgcatggc ctctacgtga
     4201 tgggactggt cgccaaggcg gcaaccgaag aagccggcgg agacccgcgg gcactgaagc
     4261 ggctttcggt ccagttccgc ggtatgagca tgccggaaac ggagatcgag gtctcgggcg
     4321 aagtgatcga ggccgacggc gccgacgtca cctttgacct gatcgcggcc cagggcgaga
     4381 accaggtgat ccggaacgcc accgccgacg tcacctactc gtaggacggc tcgcaggtcg
     4441 cggaaaacgc ggcttcatac aatgaagtga tgctctccga gcgacaggaa ctgattcttg
     4501 ggctggttgt ggatgcttac cgggaaaccg ggcgtccggt ggggtcgaag gcgattgccg
     4561 gggattcccg gctggagtgg ggtgcctcga cggtcagggc ggagcttgcc gcgcttgagg
     4621 cgggcggcta tctgacccat ccccacactt cggccgggcg ggttccgacc gacctcggct
     4681 accggcagta cgtcgaatcg ctgaagtccc gcgcggtaac ccgcggtgcg cccgcggcca
     4741 ccctcgacct cagcgacctg cgtcgcgagg tcgacggggc gatgcaggaa gcgacctcgg
     4801 ccctggccca gatcaccgac ctgctcgccc tggtcgtggc tccgccactg gggacggccc
     4861 ggatccaccg ggtcgaagcc ctgctgctgc agcccgaggt ggtgatggtg atcgtgatca
     4921 catcgaccgg tgacgtcacc cggcgggcct tcaccttcgc ctcgccagtc gatcccggtc
     4981 tggtcgagtg ggcctccagc tacctgaacg agtcgctctc cggcatcgat ctcggggctc
     5041 gccggatcgc ctcgaaactt gccgatccgg cgctcggtcc gcttgaggcc aatttcatcg
     5101 agcagcttgc gccggccctg gtcgacctcg aacagggtct cgagggcacg ctctacgtcg
     5161 atggtgcctc ccggctgctc gatcagcgca gggctgccga cctgccccgg atcgaccgcc
     5221 tgatgaccac actggaggag aaggcgaccg tgctcaggat gatgcgctcg gcaatcgacg
     5281 agcgctccgt cttcctctgg atcggggccg agaatcccca gcccgaactt caggacgtga
     5341 ccgtgatcgg cgccaattac gggctcggcc accggaacct gggctcggtc ggggtggtcg
     5401 gcccgacccg catggattac gagaaggcga tcgccagcgt cagtgaagcc gcccgcgagc
     5461 tttcccgcta cttcgaattc gtctacgacg catgagcgaa aagcgcgatt actacgagat
     5521 tctcggggta agccgggaag cgaccgacgc ggagatcaag aaggcattcc gcaaggtcgc
     5581 ccgggaactc cacccggacg tgaacgctca cgatcccgag gccgaagaga agttcaagga
     5641 ggcggccgag gcctacgagg tgctctccga cgccgagcgg cggggaacct acgaccagtt
     5701 cggtcacgac gggcttcgct ccggtggcta ccagtcgcag gcccaggggt tcggttcgat
     5761 cgacgacctg ttcagcgcct tcttcggcgg cggcttcgga gcatcgcggg gtccggcgcc
     5821 gggtgccgac gtcggggtcc gaaccgagat cgaactcggg gacgtgctcg acggcacgac
     5881 ccgcgaagtc gaattcgatg cggtcacgag ctgcgagcac tgccatggca acggggccga
     5941 accgggaacc ccgatcagga cctgcgaccg ctgcggcggc agcggggaac tgaggagcgt
     6001 cgccaacacc gccttcggcc agatggtccg ggccaccccc tgcgaccgct gccacggtga
     6061 cggccggatc cccgaggaac cctgcaaggt ctgctccggg ctgggccgca agcacaccgc
     6121 caaaaaacac cggattgaca tccccgccgg tatcgagtcc ggccagcgga tccgcatctc
     6181 cggtgccggg cacgccggtg aaaccggggc gccgcccggg gatctctatg tcgaggttca
     6241 ggtcgccgaa cgcgaaggaa tggtccgcga cggccaggac ctgatcaccg tggtaccggt
     6301 cgacgccacg gcggcgatga tcggcgacac gcttgaagtc gagacgctcg atggcgaaga
     6361 cgagatcgaa ctcgcccccg gcacccagcc cggggcggaa acgcggctca agggcaaggg
     6421 gctgccgcaa ctcggatcct cacggcgtcg cggtgatcac cgcttcatct tcaacgtgat
     6481 catcccggcc aacctcagcg agcagcagca gaaactggcc gccgacctgg atgggacgct
     6541 gaccgacgag aaccgcgaac cgaaggaatc gggcatcttc tcgcgcatgc gcagggcttt
     6601 cggttgatcc gtcttgccgt ccggtgccag ccggaacagg ccgaactcgt actggccgaa
     6661 ctcaccgttc tcgccccggg cggggtggaa gaggtcgccg ctgacggata cgtggaatac
     6721 gcgatctacg gcgccgaagg cgagctgccc gacctcggcg aggaggagaa ggtagtcatc
     6781 gccggcggca cggtggaagt cgaggcgacc gaggtcgccg atgactgggc cgatcgctgg
     6841 cgcaccttcc acaagccgct gctggtcggt gacccggacc ggggtcccgc ggtctggatc
     6901 cggccgccct gggaaccgga aagggccgac ctggccgagg tcgtggtcga cccgggccag
     6961 gccttcggca ccggagccca tccgaccacc cggctctgca tcgaggccct gatcgagatt
     7021 gccggccggg gtgatccgtt cggtcccctg accgacctcg gctgcggatc gggcgtactg
     7081 gcgatcgcgg ccggcaagct gggctggcga ccgctcgagg gctgcgacaa cgaggtcgcc
     7141 gcggtcgagg ccgcccgtga gaacgcccgg gtcaacgggg tcgaggccgg cttcgatcgg
     7201 attgacctca agtccgggct cccggccctg gcgcccacca cggtcgccaa cctgaccgcg
     7261 ccactgctgg aaatcgtcgc gaacggactg agcgcggaga acctgccgga aaccctgatc
     7321 tgctccgggc tcctgaccac cgaatacgac cgggtcctgg ccgccttcgg accccatggc
     7381 ctggtcgaga ccggccgcgg cgcgatcggc gactggggct cgctggtctt cgcccgggaa
     7441 ggttcgcggt gacccgctcg accctgccga tcggcgtgtt cgactcaggg gtaggaggct
     7501 tgaccgtgct ccatgagtgc ctgatctccc tgccggccga ggatttcctc tacctaggtg
     7561 acagcggtca cttcccttac gggacgaaga gcgccgaaga gctgcgcgat cgcgcccagg
     7621 tgatcgccac cgggctgctg gaacgcgggg tgaagatgct ggtcatcgcc tgcaactcgg
     7681 ccaccgccgc ggccggcgac gagattcgtc agctcggggc tgaagccggg gtgccggtgg
     7741 tgaccgtggt cggtccggaa gcggagatcg ccgccacgat cagcggcaac ggaaaagtgg
     7801 gcgtgctcgc gacgccggcg accgtcgcca gtggcgccta ccggcgggca ctcgattccc
     7861 tgggtcgacc gctgaccgtg accgaggtcg cggcacctga tctggcaccg atcatccagc
     7921 aaggcccgac ctttgaccag ggggttctcg acacagtccg cagctactgt gaaccgctcc
     7981 gtgaagcggg tgtcgacacc ttgatcctcg gctgtaccca ctacccgctg gttgctccga
     8041 tgctgcagcg catgctcggc cgggatgtca gcctggtcac ggccggccac gcgatcgccg
     8101 cggcaatcca gcgggaactg gcaagccgcg acgagttgac caccgaccgg atcgaaggcg
     8161 actaccgttt cctggtcacc ggtgacgttg attccttcgt cgaagccgga acccgattcc
     8221 tgcaactgcc gctggccgga cgggccgaga aggtggaact ttgaatcagg acgatcagca
     8281 aaacagccgc aacacggcaa ccgccaggtt cgccggatgg ctggcggtcg gggttctctt
     8341 catggttggc tgcatcgccc cgatcagctt cttcgactgg attccggcgg cgctgctggc
     8401 cggtttcctg atctggcggg gagttcgctc gccccgatac ttctttgcct tcgcggccgg
     8461 gatcgggatc accctggtcg gcctcgggat cgtcaacggg acctaccgca tcatgctgct
     8521 ctacgggtcg gtcgtgatcg ccgggggaat cgtcttctac gccgccttcg gcccccgtac
     8581 ttcgcatgcg gagccgcccg gcggcaaggg aagccagagc tgaaccgctt cttcacaggc
     8641 ggtgagcggg ttcggctgcc cgggcaggta cgctagtccc atgtcagccg ccgaccagat
     8701 tcgcgaagac gcacaggccg ccctcaagga cggcgaccgc aagcgtgcct cggcgcttcg
     8761 aatgatccag gacgtcctcc agcaggaaaa caagctgggc aagggcgatg agatcagcgc
     8821 cctgcagcgc gagcacaaga agcggctgga ggcagccaag gcattcgccg acgccggccg
     8881 ttacgaacag gccgaggacg aacgcttcga ggccgagctg atcgaggctt acctgccggc
     8941 ccagctgagc gacgaggaac tggccgacct tgcgaggctg gcgatcgccg aaaccggcgc
     9001 gaccgagcag aagcacatgg gccaggtgat gggtctcctg aaggaaaggg tcgccggccg
     9061 cgccgacggc aagcgggtca gcacggaggt gcgcagccag ctcaatggct gaccttatgg
     9121 cgcgtaggac tctcaccctg gacaacgatg ttgcggccga cctggccggt gccgaggatt
     9181 ccgtcctccg cgcactccgg gacagcacca cgagccgcgt gcacctgcgc ggcaaccagc
     9241 tcaccctcga aggtgatgac gcggagaacg acaaggcggc ccgcctggtc
//
LOCUS       DAOQKN010000090         9247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_3571_length_9247_cov_4.828764, whole genome shotgun sequence.
ACCESSION   DAOQKN010000090 DAOQKN010000000
VERSION     DAOQKN010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 9247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9247
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3571_length_9247_cov_4.828764"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            203..502
                     /locus_tag="PLJ64_07295"
     CDS             203..502
                     /locus_tag="PLJ64_07295"
                     /inference="COORDINATES: protein motif:HMM:NF025590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4333 domain-containing protein"
                     /protein_id="HNC93321.1"
                     /translation="MLVRPLFLLAGPFLAAMILAGCGAGDVIDDQKTEIAVRFDVQDA
                     TGEPVRSVTCPSGVPVSVGTRFTCHVVAKSGDDAVAELEVTSDDGDLRMLSLIAP"
     gene            684..2489
                     /locus_tag="PLJ64_07300"
     CDS             684..2489
                     /locus_tag="PLJ64_07300"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HNC93322.1"
                     /translation="MSETTQVTAPTKNARLLAWVDEIAELTQPDQVYWCDGSAEEYNA
                     LAGRLVEAGTFEKLSDAKRPNSYLALSDPGDVARVEDRTFICSEKEEDAGPTNNWRDP
                     EEMRATLKPFFEGSMKGRTMYVVPFSMGPLGSNIAHIGVQLTDSAYVAASMRIMTRMG
                     KGALDVLGDGEFVPCVHSVGMPLEEGQEDVPWPCNAENKYIVHYPETREIWSYGSGYG
                     GNALLGKKCFALRIASVMARDEGWMAEHMLILKLTSPEGEVKYVTGAFPSACGKTNLA
                     MLIPTMEGWTVETVGDDIAWMKFDDEGRLRAVNPEAGFFGVAPGTGADSNPNAMATIV
                     DNTVFTNCAKTDDGDIWWEGIGPAPEHLIDWHGNDWAPDSETPAAHPNARFAVPAAQC
                     PTIASEWEDPAGVPIDAFLFGGRRASVVPLVSESFDWNHGVYLAATMSSETTAAAAGA
                     VGKLRFDPMAMLPFCGYNMADYFGHWLDIGQTEGAKLPKIFYVNWFRKDEDGKFIWPG
                     FGDNSRVLEWVFNRCDDRGETVETSIGLLPKVEELDLEGVDVTPEQMEVLLTVDDEAV
                     KAQLPQIEEHLDKFGDSLPAEIRNQFEALKGRLGA"
     gene            2503..3222
                     /locus_tag="PLJ64_07305"
     CDS             2503..3222
                     /locus_tag="PLJ64_07305"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: protein motif:HMM:NF006599.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HNC93323.1"
                     /translation="MGLLGVIGGSGLYGLSASGRPKAVSTPWGPASGPTVIHEVGGGV
                     AFIARHGADHSIAPHRVNYRANIHALREAGCDRVLAVSSVGGIAPACQPGKLVVPDQL
                     IDYTWGREMTFQGEGDAVRHFDFTDPYSPDWRGKVTGALSELEIEFVDGGTYAAVQGP
                     RFETAAEIRRLAADGCHIVGMTGMPEAILAREAGLDYAAVCPVGNLAAGIATDELSMD
                     DVIGAVGTGMERVTSLITNLA"
     gene            3431..4792
                     /locus_tag="PLJ64_07310"
     CDS             3431..4792
                     /locus_tag="PLJ64_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HNC93324.1"
                     /translation="MSEDKKFLLPESAIPTGWVNIMADAPGEPLPPLSPATGQPAGPD
                     DLSAIFPMSLIEQEMSPEPVVEIPDEVRETYKLWRPTPLIRATRLEKALDTPAHIYYK
                     YEGTSPAGSHKLNSAVAQAYYNREAGTRKLVTETGAGQWGSALSLACQWFGIECEVFM
                     VGVSYDQKPYRRAMIETWGASVHRSPSDLTEAGRSQAAHESGSLGIAISEAVEVAAGS
                     EDANYALGSVLNHVCLHQTVIGQEAIAAMEMAGEYPDVVVGCVGGGSNFAGLINPFLR
                     ENIRNGKTTRFLGAEPAACPTLTRGKYAYDFGDTVGMTPLMPMYTLGHDFVPAPVHAG
                     GLRYHGDAPSLCAFVHEGFVEATAVPQGAAFEAALLFARTEGIIPGPEPAHAIRAVIE
                     EAEAAKQAGEERVILLGLSGHGHFDMSAYQAYLSGELEDLELDQEELDAAIERLPEAP
                     AIA"
     gene            4847..6082
                     /locus_tag="PLJ64_07315"
     CDS             4847..6082
                     /locus_tag="PLJ64_07315"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC93325.1"
                     /translation="MLQPGNSTASRSRLLSGGMILAALAAVLTAFAVLAAPARSADPY
                     TVESNIPYAPANAQAGSNLLDVYVPKADSAKPRPVFVWIHGGGWFTGDKAGGTMEYKA
                     KTLVDNGFIFVSVNYRLSPDLAGPKALSKGRLIFPAHYLDAARALGWVNKHIGQYGGN
                     PKKMVIGGDSAGGQISSLLATKPAFLKARGMSTRQIKGVLALDAVGYDVARMMTPAYR
                     RINSGFQRMMFNAFGTPAEEKRNHRWAAASPINFAGRTDPPIFFVVPTTSPDRWVDAH
                     VMARKLGQRLGLVSHRVKTEHAGVVPLLGNPSGDMGVTAPLMRFAHAAVNPVRYTPTV
                     RGTRIVRAGRDGYGTFRLKFSSKPAARALICQVDRGVQSVCPVAMKSPPGLHRLRVWA
                     YGNTGRAAGLTTVKLRVIR"
     gene            6079..6942
                     /locus_tag="PLJ64_07320"
     CDS             6079..6942
                     /locus_tag="PLJ64_07320"
                     /inference="COORDINATES: protein motif:HMM:NF039218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH hydrolase domain-containing protein"
                     /protein_id="HNC93326.1"
                     /translation="MSRSIRVLLLVAVAGLFALSGIATQASARTYEPSFAAVSTDKGP
                     VFREGCLIYTKRATSPPCRYGEVGSSRKVTVLGDSHALQWTPPLINLARERGWELTML
                     LHANCTAALVNIDPYCNRWRHNALKRIRREKPTLVFVASNTAPNTFVMKNGRRLGRAA
                     SEPLFRQGMYETLLKLRKYGAQVTVMRDLPMSKAFLPSVCVSENVDQPGRCTFPARRP
                     LSQAYDFAAAKRLKAVQIIDPLPKVCPGGKCRAVHGNVLKYRDRGHISATYARLLTNW
                     LNGQLQNPFKA"
     gene            7019..8221
                     /locus_tag="PLJ64_07325"
     CDS             7019..8221
                     /locus_tag="PLJ64_07325"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC93327.1"
                     /translation="MAGLMLVAVPAAAEPARVITDINYNADEVGPAQSTSNSLDLYLP
                     ATDDGFEGLRPVVVWVHGGAWMTGDKSNRMTDKVNLFNSLGYIVASLNYRLSPDISEG
                     SFDGTWDPTRVRSPDHIADVAEGIAWVSNNVRHYGGDPDRLVLAGHSAGAHLVSLAGT
                     SPAWIKGRRVSPKQIIGVVSLDTDTFDVAASADPANPANSSQQLALIWNAFGTPDEEA
                     VEARWERSSPLAFADPADPPFLLVTQGEKVLRMAANDEMATALGQDPAQSVVGVPLDH
                     EGINEALGSITDGTVETARVSQFVRKVAEGARPAGVRMVRRPARTIVVKRRGALRPGR
                     QVRFGFVGTGRASGFQCRIDRQPFTRCRSPRDYRLKPGKHVFRVRALYPSGRPGNERT
                     VTFRIVRR"
     gene            complement(8313..>9247)
                     /locus_tag="PLJ64_07330"
     CDS             complement(8313..>9247)
                     /locus_tag="PLJ64_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HNC93328.1"
                     /translation="MAAKVPDMAKQADILLGNLEDAVPVDNKEAARAGLVKVAKEADL
                     GGTPLWTRINSLESPWVLDDLTTLVTEVGNKIDVIMVPKVEGPWDIHYVDQLLAQLEA
                     KGEVTRPILIHAILETAQGVTNVEDIAAASPRMQGMSFGPADLAASRRMKTTRVGGGH
                     PGYRSMEDPDAGNPEAARVTAQQDPWHYSIARMVDACTANDILPFYGPFGDIKDLEGC
                     ETQFRSAFLLGCVGAWSLHPAQIAVAKKVFSPPVDEVIFAKKVIEAIPDGRGVHMIDG
                     KMQDDATWKQCKVMVGLAEMLAEKDPELKEQYGF"
BASE COUNT         1569 a         3074 c         2990 g         1614 t
ORIGIN      
        1 cggcgatctt ccgtgcctct tcgggggttt ggcagaagcc gtacttggtg accggaattc
       61 cggccagctc gacgatcttt cttgcctcgt attcgtagaa gatcattgcg gcgaaaatct
      121 attccagaac cgggcgaccg gttattccga ttgagtcgct aggaatcggc ggcgcgaccg
      181 gacgctatgt agtgtgcggc ccgtgttggt ccgtcctctc ttcctgctcg ccggcccgtt
      241 cctggccgcc atgatccttg ccggctgcgg agccggtgac gtgatcgacg atcagaagac
      301 cgagatcgcg gtccggttcg acgtgcagga cgccaccggg gagccggtgc gctcagtgac
      361 ctgcccgtcc ggggtgccgg tctcggtcgg cacccgcttc acctgtcacg tggtggcgaa
      421 gtcgggcgat gatgccgtgg ccgaactcga agtcacctcc gatgacggcg atttgcggat
      481 gctgtcactc atcgccccct gaattggcca aaccagtggt ggtaaccggc ggagtcgtct
      541 gtgaatcatt gtcggtagtt ctccgcagac cctcgctggt cagaccgcaa ttcgcactga
      601 tctccgtcac aattccgtaa ccggctaaat ccgcgccgcc ctctccttcg caggcgcgtt
      661 gaccaaggag ttgaagagcg ccagtgagtg aaacgaccca ggtgaccgcc ccgaccaaga
      721 acgccaggct gcttgcctgg gtggatgaga tcgccgagct gacccagccc gatcaggtct
      781 actggtgtga cggttccgcc gaggagtaca acgccctcgc cgggcgcctg gtggaagccg
      841 ggaccttcga aaagctctct gacgcaaagc gccccaactc gtacctcgcc ctttcggatc
      901 ccggtgacgt cgcccgggtc gaggaccgaa ccttcatttg ttcggagaag gaagaagacg
      961 ccgggccgac caacaactgg cgcgaccccg aggagatgcg ggccaccctg aagccgttct
     1021 tcgagggctc gatgaagggc cgcaccatgt acgtggtgcc gttctcgatg ggcccgctcg
     1081 gctcgaacat cgcccacatc ggtgttcagc tcaccgactc ggcctacgtc gccgcgtcga
     1141 tgcggatcat gacccggatg ggcaagggcg cgctcgacgt gcttggtgat ggcgagttcg
     1201 ttccctgcgt ccactcggtc ggcatgccgc tcgaggaagg ccaggaagac gtgccgtggc
     1261 cctgcaacgc cgagaacaag tacatcgtcc actacccgga gacccgggag atctggtcat
     1321 acggttccgg ctacggcggc aacgcgctgc tcggcaagaa gtgtttcgcg ctgcggatcg
     1381 cctcggtgat ggcccgcgac gagggctgga tggccgagca catgctgatt ctcaagctga
     1441 ccagccccga aggcgaggtc aagtacgtga ccggtgcctt cccctcggcc tgcggcaaga
     1501 ccaatctcgc gatgctgatt ccgacgatgg agggctggac cgtggagacg gtcggcgacg
     1561 acatcgcctg gatgaagttc gatgacgagg gccgcctgcg cgccgtcaat cccgaggccg
     1621 gcttcttcgg ggtcgccccc ggcaccggtg ccgactccaa cccgaacgca atggcgacca
     1681 tcgtcgacaa cacggtcttc accaactgcg cgaagacgga cgatggcgat atctggtggg
     1741 agggcatcgg ccccgctccc gagcacctga tcgactggca cggcaatgac tgggcccctg
     1801 attcggagac cccggcggcc cacccgaacg cccgcttcgc cgtccctgcc gcgcagtgcc
     1861 ccaccatcgc ctccgagtgg gaggatccgg ccggcgtgcc gatcgacgcc ttcctctttg
     1921 gtggccgccg ggccagcgtg gtgccgctcg tttccgagtc ctttgactgg aatcacggcg
     1981 tttacctcgc cgcaaccatg tcttccgaga ccacggcagc tgctgccggc gcggtcggca
     2041 agctgcgctt cgacccgatg gcgatgctgc ccttctgcgg gtacaacatg gccgactact
     2101 tcggccactg gctcgacatc ggccagaccg agggggcgaa gctgccgaag atcttctatg
     2161 tcaactggtt ccgcaaggac gaagacggca agttcatctg gcctggattc ggcgacaaca
     2221 gccgcgtgct cgagtgggtc ttcaaccgct gcgacgaccg gggcgagacg gtcgagacct
     2281 cgatcgggct gctgcctaag gtcgaggaac tcgatctcga aggcgtcgac gtgaccccgg
     2341 aacagatgga agtgctgctg accgtggacg acgaagcggt caaggcacag ttgccccaga
     2401 tcgaagagca tctggacaag ttcggcgatt cactgccggc cgagatccgg aatcagttcg
     2461 aagccctcaa gggccggctc ggagcctgac ggcagaactg ccgtgggcct gctcggggtc
     2521 atcggcggca gcggtctcta cggcctgagc gcctcaggcc ggcccaaggc tgtttcaacc
     2581 ccgtggggcc cggcatcggg ccccacggtc attcacgagg tcggaggcgg ggttgcgttc
     2641 atcgcccgcc acggggccga tcattcgatc gccccgcatc gggtcaatta ccgggccaac
     2701 atccacgccc tgcgtgaggc cggctgcgac cgggtcctcg ccgtttccag cgtcggcggg
     2761 atcgctccgg cctgtcagcc gggaaagctt gtcgtccccg atcagctgat cgattacacc
     2821 tggggtcgcg agatgacctt ccagggcgag ggcgatgcgg tccgccattt cgatttcacc
     2881 gatccctatt cacccgattg gcgcgggaag gtcaccgggg cgttgagcga gctggagatc
     2941 gaattcgtcg acggcggcac ttacgccgcg gtccagggac cgcggtttga aaccgccgcg
     3001 gaaatccggc gcctcgctgc cgacggctgc cacatcgtcg gcatgaccgg catgcccgaa
     3061 gccatcctcg cccgcgaggc cggcctcgat tacgccgctg tctgtccggt tggaaacctc
     3121 gcggccggca tcgccaccga cgaactcagc atggacgacg tgatcggcgc cgtcggcacc
     3181 ggcatggaac gggtcaccag cctgatcaca aatctcgcct gaccagctct ggcggtgacg
     3241 cccgcggctg ctccaaaccg ggagatgcct cgcttcgcgc gtctttgaac tccaaggtgc
     3301 cgcaggtttg ctaccggaga ggtcgcaaac ctgcggcacc gtcgtatcgg aggggtttca
     3361 gttggaaggc ggggcgaacc ccgcccgacc tgcttatgtg ggaatgccgg acagccgtaa
     3421 aatgtccgtc atgagtgagg acaagaagtt tctgctgcct gagtctgcga ttcctaccgg
     3481 ctgggtcaac atcatggccg acgctccggg cgagcccttg ccgccgctca gcccggccac
     3541 cggccagccg gccgggcccg atgacctctc ggcgatcttc ccgatgagcc tgatcgaaca
     3601 ggagatgtca ccggagccgg tggtcgagat tcccgatgaa gtgagggaga cttacaagct
     3661 ctggcggccg accccgctga tccgggcgac ccgcctcgag aaggctctcg acacccccgc
     3721 tcacatctat tacaagtacg aaggcacttc cccggccggc tcccacaagc tgaactcggc
     3781 cgtcgctcag gcctattaca accgggaagc cggcacccgg aaactggtta ccgaaaccgg
     3841 ggcggggcag tggggctcgg ccctctcgct cgcctgccag tggttcggga tcgaatgcga
     3901 agtgttcatg gtcggggttt cctacgacca gaagccctat cgccgggcga tgatcgaaac
     3961 ctggggtgcc tcggttcacc gcagcccctc ggacctgacc gaagcgggcc gttcccaggc
     4021 agctcacgag agcggcagcc tcggcatcgc gatctcggaa gcggtcgagg tcgcggccgg
     4081 ctccgaggac gccaactatg ccctgggttc cgtcctcaat cacgtctgcc tccaccagac
     4141 cgtgatcggc caggaagcga tcgccgcgat ggagatggcc ggcgaatacc cggatgtggt
     4201 ggttggctgc gtcggtggcg gctcgaactt cgccggcctg atcaacccct tcctgcgcga
     4261 gaacatccga aacggcaaga cgacccgctt cctcggcgcg gaaccggccg cctgcccgac
     4321 cctgacccgg ggcaagtacg cctatgactt cggtgacacg gtaggcatga ctccgctgat
     4381 gccgatgtac acgctcggtc atgacttcgt gcccgcaccc gttcacgcgg gaggcctcag
     4441 gtatcacggt gatgccccca gcctctgcgc cttcgttcac gagggtttcg tcgaggcgac
     4501 cgccgttccc cagggtgcgg ccttcgaggc ggcgctgctc ttcgcccgca ccgaagggat
     4561 cattcccggc ccggaaccgg cccatgcgat ccgcgccgtc atcgaggaag ccgaagcggc
     4621 gaagcaggcc ggtgaggagc gggtgatcct gctcgggctt tccggccacg gtcatttcga
     4681 catgtccgcc tatcaggcgt acctgtcggg agagcttgag gatctcgaac tcgatcagga
     4741 ggaactggac gccgcgatcg agcgcctgcc ggaggcaccg gcgatcgcct gaaatttccc
     4801 gggaccgggc gcacccccga ccggtaaagg ttgttaacta ccccgaatgc tccagccggg
     4861 gaactccaca gccagtcgat cgcgcctgct ttccggaggc atgatcctcg ccgctctggc
     4921 ggccgttttg actgctttcg cggtcctggc ggcgcctgcg aggtcggccg atccctacac
     4981 ggtcgaatcc aacattccct acgcgcccgc caacgcccag gctggctcca acctgcttga
     5041 cgtctacgtc ccgaaggccg attcggccaa accgcggccg gtcttcgtct ggattcacgg
     5101 tggcggctgg ttcaccggtg acaaggccgg tggaaccatg gaatacaagg cgaagaccct
     5161 ggttgacaac ggattcatct tcgtttcggt caactaccgg ctttcccctg atctggccgg
     5221 cccgaaggcg ctctcgaagg gccgtctgat cttcccggct cattacctgg acgctgcccg
     5281 cgcgctgggc tgggtcaaca aacacatcgg ccagtacggc ggaaatccga agaagatggt
     5341 gatcggcggt gattcggccg gcggacagat ctcgagcctg ctggcgacca agcccgcgtt
     5401 cctcaaggcg cgggggatgt ccacccgcca gatcaagggc gtgctcgcgc tcgatgcggt
     5461 cggctacgac gttgcgcgga tgatgacgcc ggcctaccgg aggatcaact cggggttcca
     5521 gcggatgatg ttcaacgctt tcggaactcc cgcggaggaa aagaggaatc accgttgggc
     5581 ggccgcttcg ccgatcaact tcgccggccg gactgatccg ccgatctttt tcgtggtccc
     5641 gaccaccagc ccggaccgct gggtggatgc ccacgtgatg gccagaaagc tgggccagcg
     5701 actgggcctc gtctcccacc gggtcaagac cgaacatgcc ggcgtcgtgc cgctgctcgg
     5761 caatccgtcc ggtgacatgg gagtcaccgc accgctgatg cggttcgccc acgcggcggt
     5821 caacccggtc cgctacaccc cgaccgtccg cggtacgcgg atcgtgaggg ccggacggga
     5881 cggatacgga accttccggc tgaagttcag ctcgaagccg gccgcccggg ccctcatatg
     5941 ccaggtcgac cgcggcgtcc agtcggtatg tccggtcgcg atgaagtcgc cgcccggtct
     6001 tcaccggttg cgggtctggg cctatggcaa caccggtcgc gctgccggtc tgacgacggt
     6061 caagctgcgg gtgatccggt gagccgttca atccgtgttc tgctgcttgt ggccgtggcc
     6121 ggccttttcg cgctttccgg gatcgccacg caggcttcgg cccgcaccta cgaaccctcg
     6181 tttgccgcgg taagtaccga caagggcccg gtcttccgcg agggttgtct gatctacaca
     6241 aagcgggcca cctcgccgcc ctgccgttac ggcgaggtcg gttccagccg caaggtgacg
     6301 gtcctcggcg actcgcatgc cctgcagtgg actccaccgc tgatcaacct cgccagggaa
     6361 cggggctggg agctgaccat gctgcttcat gccaactgca ctgccgcatt ggtcaatatc
     6421 gatccctact gcaaccgctg gcgtcacaac gcactgaagc ggatcaggcg ggagaagccg
     6481 accctggttt tcgtcgcgag caacaccgcc cccaacacct tcgtgatgaa gaacggcagg
     6541 cggctgggcc gggcagccag cgaacccctt ttccggcagg ggatgtacga gaccctgctg
     6601 aagctgcgca agtacggagc gcaggtgacg gtgatgcgtg acctgcccat gtcaaaggcc
     6661 ttcctgccct cggtctgcgt ttcagagaac gtcgaccagc ccggacgatg caccttcccg
     6721 gcccgccggc ccctttccca ggcctacgat ttcgccgctg cgaaacggct caaggcggtg
     6781 cagatcatcg atccgctgcc caaggtctgc cccggaggca agtgccgtgc cgtccacggg
     6841 aacgtcctca agtaccgcga ccgcggccac atcagcgcca cctacgcccg cctgctgacc
     6901 aactggctga acggtcagct tcagaatccg ttcaaggcct gaggccgctc cgttggcccc
     6961 ggagccgggt tttccgggta tgaccggtcg gatcacagcc gcaatctgtt cgctcgcgat
     7021 ggcgggtctg atgctggtcg cggttccggc cgcagccgaa ccggcaaggg taatcaccga
     7081 catcaactac aacgccgacg aggtggggcc ggcgcaatcg acctcgaaca gtctcgatct
     7141 ctacctgccg gccaccgacg acggcttcga aggtctgcgt ccggtcgtgg tctgggttca
     7201 cggcggggcc tggatgaccg gggacaagtc aaaccggatg accgacaagg tgaacctgtt
     7261 caactcgctc ggctacatcg tggccagcct caactaccgg ctctcgcccg acatatcgga
     7321 gggcagcttt gacggaacct gggacccgac cagggtccgc tcgccggatc acatcgccga
     7381 cgttgccgaa gggatcgcct gggtgtcgaa caacgtccgc cactacggcg gcgaccccga
     7441 ccggctggtc ctggccgggc attcggcggg agcccatctg gtttccctgg ccgggacgag
     7501 cccggcctgg atcaagggcc gccgggtctc accgaagcag atcatcgggg tcgtttcact
     7561 ggacaccgac accttcgacg tggccgcttc ggcggacccc gccaatccgg ccaattcaag
     7621 ccagcagctc gccttgatct ggaatgcctt cggcacaccg gatgaagaag cggtcgaggc
     7681 acgctgggaa cgctcctcgc cactcgcctt cgccgatccc gctgacccgc ccttcctgct
     7741 ggtaacccag ggggaaaagg tcctgcgaat ggccgccaac gacgagatgg cgaccgccct
     7801 cggccaggac cccgcccaat cggtggtcgg ggtgccgctc gaccacgagg ggatcaacga
     7861 ggcgctgggt tcgatcaccg acgggaccgt cgaaaccgcc cgggtgagcc agtttgtccg
     7921 gaaggtcgcg gaaggcgcca ggccggccgg ggtcaggatg gtccggcgcc cggccaggac
     7981 gatcgtggtc aagcgccgcg gggccctaag gcccgggcgc caggtcaggt tcggcttcgt
     8041 cggcaccggc cgggccagcg gcttccagtg ccggatcgac cgccagccgt ttactcgctg
     8101 ccgcagccct agggactacc gcctgaaacc cggcaaacac gtcttccggg tgcgagcgct
     8161 ctacccgagc ggaaggccgg gcaacgagcg gacagtgacc ttccgcatcg tgcgccgata
     8221 ggcggagccc ctcccaaccg tcattttctt cgagtgggag gggcgggttc gaattgaagc
     8281 gccggcgaag accggcgcga ctgcagttaa gttcagaagc cgtattgctc cttgagctcc
     8341 ggatccttct cggccagcat ctcggccagg ccgaccatca ccttgcactg cttccaggtg
     8401 gcgtcatcct gcatcttgcc gtcgatcatg tgaacaccgc ggccgtccgg gatcgcctcg
     8461 atcaccttct tggcgaagat cacttcgtcg accggcggcg agaagacctt cttcgcgacc
     8521 gcgatctggg ccgggtggag cgaccaggcg ccgacgcagc cgagcaggaa ggccgagcgg
     8581 aactgggttt cgcagccttc gaggtccttg atgtcaccga atgggccgta gaacgggagg
     8641 atgtcattgg ccgtgcaggc atcgaccatg cgggcgatcg agtaatgcca cggatcctgc
     8701 tgggcggtga cgcgggccgc ctcggggttc ccggcgtccg ggtcctccat cgagcggtag
     8761 cccgggtggc cgccgccgac ccgggtggtc ttcatgcggc gggaggcggc gaggtcggcc
     8821 ggtccgaagc tcatgccctg catgcggggg ctggccgcgg cgatgtcctc gacgttggtc
     8881 acgccctggg cggtctcgag gatggcgtgg atcaggatcg gccgggtcac ttcgcccttg
     8941 gcctcgagct gggcgagcag ctggtcgacg taatggatgt cccatggacc ttcgaccttc
     9001 gggaccatga tcacgtcgat cttgttgccg acttcggtga ccagggtggt caggtcatcg
     9061 aggacccagg ggctttcgag cgagttgatg cgggtccaga gcggggtgcc gccgaggtcg
     9121 gcctctttgg cgaccttgac caggccggcg cgggcggctt ccttgttgtc gaccgggacc
     9181 gcgtcctcga ggttgccgag caggatgtcg gcctgcttgg ccatgtcagg caccttcgcc
     9241 gccatct
//
LOCUS       DAOQKN010000091         9202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_3597_length_9202_cov_6.706242, whole genome shotgun sequence.
ACCESSION   DAOQKN010000091 DAOQKN010000000
VERSION     DAOQKN010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 9202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9202
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3597_length_9202_cov_6.706242"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="PLJ64_07335"
     CDS             complement(<1..519)
                     /locus_tag="PLJ64_07335"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936082.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNC93329.1"
                     /translation="MSESNGNNTGRIEEIQGVVVDVLFPDKLPEIYSAVEIQVKASEG
                     REDQNLICEVQQHLGDDRVRTVAMDATDGLRRGDLVVDTGGPITVPVGDVTLGRIFNL
                     LGEPIDGGDALPADVERWPIHRPSPEATDLTPNQEILETGIKVVDLLAPYVKGGKIGL
                     FGGAGVGKTVLIM"
     gene            complement(554..1474)
                     /locus_tag="PLJ64_07340"
     CDS             complement(554..1474)
                     /locus_tag="PLJ64_07340"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HNC93330.1"
                     /translation="MANRKEVTNQITSVKNIRQITRAMEMVAAARLRRAETRIQHLRP
                     YAQAMRRMTQRAADQAGAVTQVPILEQRETVKRVALLLVTGDRGLAGAFNANILRDGL
                     NRRDELKAEGIETVFYAVGRRGDGALTFRKAEPKQSWTGFTDRPAFSDAREIGETLAS
                     AYVDGEVDRVEVIYNRYVSPLTQHVHRLILLPVQEAEVIGEGTEDEGGEEEGGELAEA
                     HARSSWEYEPEAEQLLATVIPDYVNTSLYRNLLESAASELGSRMTAMRSAAENAETMI
                     DDLTLEMNRVRQAEITQEILEVVGGAEALG"
     gene            complement(1519..3159)
                     /gene="atpA"
                     /locus_tag="PLJ64_07345"
     CDS             complement(1519..3159)
                     /gene="atpA"
                     /locus_tag="PLJ64_07345"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNC93331.1"
                     /translation="MQIKPDEIASILRQRIEGIDPGSADLTEVGTVLSVADGIARIHG
                     LDNCMALEMLDLPHGVTGLALNLEADNVGAVLFGEWDKIVEGDTVKRTGRLLEIPVGE
                     EFLGRMVDPLGRPLDGKGDVKSTETRPAEFKAPGVISRQPVREPMQTGIKAIDGMIPI
                     GRGQRELIIGDRQTGKTAVTIDSIINNKDSGVISVYVAIGQRMSTIVQVREVLEAAGA
                     MDNTIIVAAPADEAAPIKFIAPYAGTAMAEYFLYKGGHALCIYDDLTKHAYAYRQMSL
                     LLRRPPGREAYPGDVFYLHSRLLERAVKLSDAEGGGSLTSLPIIETQANDVSAYIPTN
                     VISITDGQIFLESDLFNSGVRPAINVGISVSRVGGDAQTKAMKKVAGKMKGELSQYRD
                     LAAFAQFGSELDAETQRTLARGERLVELLKQNERDALSVADQVASIYSGTGGYLDLIK
                     TERVQDFLVDLLARLHSDNKALLDRINETGQFPEEDEKELGDAIGNFVDDFGPDFDEN
                     GEALAEGESVRVDSAFAEEPDSAGKEEVIENEEAPSAA"
     gene            complement(3177..3710)
                     /gene="atpH"
                     /locus_tag="PLJ64_07350"
     CDS             complement(3177..3710)
                     /gene="atpH"
                     /locus_tag="PLJ64_07350"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HNC93332.1"
                     /translation="MEELARVYAESLFGVAREKGNLDEVQAELAQFSDALEGNRELAV
                     FFFSPYFSSKEKLDGLNKAVEGTTPEFRNFLELLIEKHRMPAIFRIRRDFDDLWKKEN
                     DRIEVTVTSAVELDPAVSREVADAVEKQTGKQVDLTSRVDEGIIGGLVLQVGNMVLDS
                     SIRNNLEKLRQSVAQAA"
     gene            complement(3710..4300)
                     /gene="atpF"
                     /locus_tag="PLJ64_07355"
     CDS             complement(3710..4300)
                     /gene="atpF"
                     /locus_tag="PLJ64_07355"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNC93333.1"
                     /translation="MEPVLSHIATVLPLAAEAAEEEGGSFLVTPGLGLMIWTLVLFGF
                     TFWVLKKFAFPLIGEALDKRAATIRESIEAAEKQKEESDKLLADYRQRLTEAREQADE
                     IVTKARKAAETTKSEASAEGQAKREELVDAARKDIEAEKTKALDDIRKEVANLTVLAT
                     ERVTRKSLDDAAHKELVEDALREVDFSALAGEERNN"
     gene            complement(4308..4577)
                     /gene="atpE"
                     /locus_tag="PLJ64_07360"
     CDS             complement(4308..4577)
                     /gene="atpE"
                     /locus_tag="PLJ64_07360"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HNC93334.1"
                     /translation="MDPSSIVPLAEVTGDVTDAGKAIALGVGAGLGSIGAGIGIGFIF
                     GKEIESVARQPELASELQSIRWLGFALTEAVAFYTFIFALIAFFL"
     gene            complement(4596..5555)
                     /gene="atpB"
                     /locus_tag="PLJ64_07365"
     CDS             complement(4596..5555)
                     /gene="atpB"
                     /locus_tag="PLJ64_07365"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNC93335.1"
                     /translation="MSEVATHEQMPDTKTGFSTKAKVFFGIGIWLAVAIAFGLIFGSA
                     GKNEEFKPQNEFKLDPWINIKLGGIDLSINRAVLYLFLASMLTITVMVWIARKMQQKP
                     NRIQTAIEVAYDVTKNDITGNNIDDERLAKKWFPFLASLFFFIFFSNMLGYLPLPTNT
                     HESINIFGLEIPAFALYAATANIAIPLVLTLIVWVSYHVEGIRAKGFIKYLASWLPSG
                     LEDMNPFGKAAIFLIEVVSHFVRLISLSVRLFANILAGHLLLLFMGGGLAVLLGIAAL
                     GVLTYPMAFAFYVFEVGIVATLQAFIFSILTAIYIGGATAESH"
     gene            complement(5552..5989)
                     /locus_tag="PLJ64_07370"
     CDS             complement(5552..5989)
                     /locus_tag="PLJ64_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93336.1"
                     /translation="MTTYAAKTSLEGEMGNRSNSLIANLDLLVLVLALPVFVIAGFDL
                     AGYAVAAVVWSAQRLIQVWAERQARHKLAAGERTRAMGFVASTSLARPWLMSAVVLVA
                     CIVDREAGLYAAALLVILFTVNLAARGLAFLLGKDEKPEEAAR"
     gene            complement(6101..7186)
                     /locus_tag="PLJ64_07375"
     CDS             complement(6101..7186)
                     /locus_tag="PLJ64_07375"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNC93337.1"
                     /translation="MVEGIDAVWAFLTAALVTLLLVRPAERLARRVDAIDEPGPRSQH
                     TSPTPRMSGLAILIGIQVAGWIWLPISTETLSILLGACVVSLVGALDDMFDLPAGLKL
                     LGQLGAATIPVVGGVYPANLTVPFFGGFELGWVAYPLTVLGIALVMNIVNLIDGIDGL
                     AAGFCAIAAAAMAIIAVSLQRYEAGVLAAMTAGGAIGFLRYGFPPASSFMGDTGSNLL
                     GYMIAVASVQGALKTNAVIAMVFPLVILAVPLFDTAFVVAKRLKYRQPIFQADRWHFH
                     HRMANIGFSQRRTLLYLYGWTLVLALLALALRFVPYSDDRGNFDPLWTTIMAVVLLAA
                     LAVTVYLAFVLEIIKRPRWRDEQNGED"
     assembly_gap    7300..7399
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7467..8474)
                     /locus_tag="PLJ64_07380"
     CDS             complement(7467..8474)
                     /locus_tag="PLJ64_07380"
                     /inference="COORDINATES: protein motif:HMM:NF015940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF354 domain-containing protein"
                     /protein_id="HNC93338.1"
                     /translation="MRVWFDCTAAAHPVVLRPVIERFQARGDEILVTARENGETVGNL
                     ERLGIPHLVIGRHGGAGRLGKALALGSRSARLARPVWKFRPDLAVAHGSVDLAVLSQT
                     FFIPSAQMQDYEFAGLQRKIAWRVAKRVLVPDSIPVERLVKAGARENKLVRFPGLKED
                     YYLAGFEPDPTVPEALGIDLERVLVIVRPPPETAAYHADNPLYEKVIDRLAGADGVSA
                     VIIPRTEGQRERARARNSTSLIVPDRAVDAQSLIAYADLVVSAGGTMNREAVALGTPV
                     YTTFAGRMGGVDEALIADGSLKLLESAEQLELVKRKDQTGPKNPRDPDFLVQGMLEAL
                     A"
     gene            complement(8521..>9202)
                     /locus_tag="PLJ64_07385"
     CDS             complement(8521..>9202)
                     /locus_tag="PLJ64_07385"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936093.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNC93339.1"
                     /translation="DSVGAKLMVDMAHFAGLVAAGEHPNPVPYADVVTTTIHKTLGGP
                     RSGMILCTEELKADVNRAVFPGQQGGPLMHVIAAKAVALGIASTDVFAERQRRTVENA
                     RALAAGLIDGGVDVLTDGTDVHLVLVDLTSTGLDGQTGEDRLDQVGITVNRNAIPFDE
                     RPPMNPSGLRIGTPALSTRGFTPEDMTEIARIISTALGPDFESERDSLAASSQALMDK
                     YPLYPGLV"
BASE COUNT         1553 a         2852 c         3038 g         1659 t
ORIGIN      
        1 catgatcaga acggtcttgc cgacgccggc gccgccgaac aggccgatct tgccgccctt
       61 cacgtacggg gcgaggaggt cgacgacctt gatcccggtt tcgaggatct cctggttcgg
      121 ggtcaggtcg gtcgcttccg gggacggacg gtggatcggc cagcgctcga cgtcggccgg
      181 cagcgcgtcg ccgccgtcga tcggctcgcc aaggaggttg aagatgcggc ccagcgtgac
      241 gtcgccgacc ggaacggtga tcggtccgcc ggtgtcgacc acgaggtcgc cgcgacgcag
      301 accgtcggtg gcgtccatgg ccacggtgcg tacccggtcg tcaccgaggt gctgctggac
      361 ttcgcagatc aggttctggt cttcgcggcc ctcgctggcc ttgacctgga tctcgacggc
      421 cgagtagatc tcgggcagtt tgtcggggaa gagcacgtcg accacgacgc cctgaatttc
      481 ctcgattcgt ccggtgttgt tgccgttgct ttctgacata tctccagttc cagtttcagt
      541 cggtagcgtc gcgttaaccc agcgcctcgg cgccgcccac gacttccaga atttcctgtg
      601 tgatctcggc ctgacggacc cggttcatct ccagggtcag gtcgtcgatc attgtttccg
      661 cgttctcggc cgcgctgcgc atggccgtca tccgggaccc gagttcggat gctgccgact
      721 cgagaaggtt gcggtaaagc gaagtgttca cgtaatcggg gatgaccgtt gcgagcagtt
      781 gctcggcttc cggctcgtat tcccaggagg agcgggcgtg ggcttccgcg agctcgccgc
      841 cctcttcttc cccgccttcg tcttcggttc cttcaccgat gacttcggcc tcctggaccg
      901 gcagcaggat caggcgatgg acgtgctgtg tgaggggcga gacatagcgg ttgtagatga
      961 cctcgacccg gtcgacctcg ccgtcgacgt aggccgaggc gagcgtctcg ccgatctcgc
     1021 gggcgtccga gaaggccgga cggtcggtga aaccggtcca ggactgcttc ggctccgcct
     1081 tgcggaaggt gagggcaccg tcgccacgac ggcccaccgc gtagaagacc gtctcgattc
     1141 cttcggcctt cagctcgtcg cggcggttga gcccgtcccg gaggatgttg gcgttgaagg
     1201 caccggccag accacggtca ccggtgacca ggagcagggc gacgcgcttg acggtctccc
     1261 gctgctcgag gatcggcacc tgggtgaccg ccccggcctg gtcggccgca cgctgcgtca
     1321 tgcgccgcat cgcctgtgcg taggggcgca gatgctggat ccgcgtctcc gcccggcgca
     1381 gccgcgcggc agccaccatc tccatcgcgc gcgtgatctg gcggatgttc ttgacgctgg
     1441 tgatctggtt tgtgacttct ttgcggtttg ccatctagtg cttccgtatt gccgtgaggc
     1501 ggaaaggtcc gtttccgcct aggcggcgga gggagcctcc tcgttctcga tgacttcttc
     1561 cttgccggcc gagtcgggct cttcggcgaa ggccgaatca acccgtaccg actcgccctc
     1621 ggcaagcgct tcgccgttct cgtcgaaatc gggtccgaag tcgtcgacga agttgccgat
     1681 cgcgtcgccc agttcctttt cgtcttcttc cgggaactgg ccggtctcgt tgatccggtc
     1741 gagcagggcc ttgttgtccg agtggagccg ggccagcagg tcgaccagga agtcctggac
     1801 gcgctcggtc ttgatcaggt cgaggtaacc accggtgccg gagtagatcg aagcgacctg
     1861 gtcggcgacc gaaagggcat cgcgctcgtt ctgcttgagc agctcgacca gacgctcgcc
     1921 gcgggccagc gtgcgctggg tttccgcgtc gagttcggag ccgaactggg cgaaggcggc
     1981 gaggtcgcgg tactgcgaca gctcgccctt catcttgccg gcgaccttct tcatcgcctt
     2041 ggtctgggcg tcaccaccga cccgcgacac cgagatgccg acgttgatgg cggggcggac
     2101 gcccgagttg aagaggtccg actcgaggaa gatctggccg tcggtgatcg agatgacgtt
     2161 ggtcgggatg taggccgaca cgtcattggc ctgggtctcg atgatcggca gcgaggtcag
     2221 cgatccgccg ccctccgcgt cgctcagctt gaccgcccgt tcgagcaggc gcgagtgcag
     2281 gtagaagacg tcgccggggt acgcctcgcg tcccgggggg cgccggagca gcagcgacat
     2341 ctgacggtac gcgtaggcgt gcttggtcag gtcgtcgtag atgcagagag cgtggccgcc
     2401 cttgtagagg aagtactcgg ccattgccgt gccggcgtag ggcgcgatga acttgatcgg
     2461 ggccgcctcg tcagcgggag cggcgacgat gatggtgttg tccatcgccc cggccgcctc
     2521 gagcacctcg cggacctgga cgatcgtcga catccgctgg ccgatcgcga cgtagaccga
     2581 gatcactccc gagtccttgt tgttgatgat cgagtcgatc gtgacggcgg tcttgccggt
     2641 ctggcggtcg ccaatgatca gctcgcgctg gccacggccg atcgggatca tgccgtcgat
     2701 cgccttgatg ccggtctgca tcggctcacg cacgggctgg cggctgataa cgccgggcgc
     2761 cttgaactcg gccggtcggg tctcggtcga cttgacgtca cccttgccgt cgagcggacg
     2821 gccgagcggg tcgaccatgc ggccgaggaa ttcctcgccg accgggatct cgagcaggcg
     2881 gccggtgcgc ttcaccgtgt cgccttcgac gatcttgtcc cactcgccga agagcacggc
     2941 gccgacgttg tcggcttcga ggttcagcgc gagtccggtt accccgtggg gcaggtcgag
     3001 catctccagc gccatgcagt tgtcgagccc gtgaatacgg gcgataccgt cagcgaccga
     3061 gagcacggtg ccgacctcgg tcaggtcggc gctaccgggg tcgatgccct cgatgcgctg
     3121 ccgcagaatg ctggcaatct cgtctggctt gatctgcatc tcggtccaaa tcctccttag
     3181 gccgcctgag ccacgctctg gcggagtttc tccaagttgt tgcgaatgct gctgtcgaga
     3241 accatgttcc cgacctgaag tacgagtccg ccgatgatcc cttcatcgac gcggctggtc
     3301 agatcgacct gcttgccggt ctgcttttca accgcgtcgg cgacttcccg ggacacggcc
     3361 ggatcgagct cgaccgcact ggtcacggtc acttcgatcc ggtcgttttc cttcttccag
     3421 aggtcatcga aatcgcgccg gatccggaaa atcgccggca tccggtgctt ttcgatgagc
     3481 aactcgagga agttgcggaa ttcgggcgtg gtcccctcga ccgccttgtt caggccgtcg
     3541 agcttctcct tcgaggagaa gtagggactg aagaagaaga ccgcgagctc gcggtttcct
     3601 tccagggcat cgctgaactg tgcaagctcg gcctgcactt cgtccaggtt gcccttctcc
     3661 ctggctacgc caaagaggga ctcggcatat acccgagcca gttcttccat cagttgttcc
     3721 gttcctcgcc ggccagggcg gagaagtcga cttcgcgcag ggcgtcctcg accagttcct
     3781 tgtgggcggc atcgtcgagc gacttgcggg tgacccgctc ggtcgcgagg acggtcaggt
     3841 tggcgacttc cttgcgaatg tcgtccaggg ccttggtctt ctcggcctcg atgtccttgc
     3901 gggcggcgtc gaccagctct tcgcgcttgg cctgcccttc cgcacttgct tcggacttgg
     3961 tcgtctcggc ggccttgcgg gccttggtga cgatttcgtc ggcctgctca cgggcttcgg
     4021 tgagtcgctg acggtaatcg gccagcagct tgtccgactc ttccttctgc ttttcggcag
     4081 cctcgatgct ctcgcggatc gttgccgcgc gcttgtcgag ggcttcgccg atcagcggga
     4141 aggcgaactt cttcagcacc cagaaggtga agccgaagag caccagggtc cagatcatca
     4201 gacccagccc cggcgtcacc aggaagctgc cgccctcttc ctcggccgct tcggctgcca
     4261 gaggcagcac ggtcgcgata tgtgagagaa caggctccac ggtcggccta caggaagaac
     4321 gcgatcagcg cgaagatgaa ggtgtagaag gcgacggcct cggtcagagc gaagccgagc
     4381 cagcggatcg actggagctc ggaagcgagc tcgggctgac gggcaaccga ctcgatctcc
     4441 ttgccgaaga tgaagccgat gccgatgccg gcgccgatcg aaccgagtcc ggcgcctacg
     4501 ccgagagcga tcgccttgcc ggcgtcggtg acgtcaccgg tgacttcggc gaggggcacg
     4561 atggaactgg gatccattac tgggactcct tttggttagt gactttcggc ggttgctccg
     4621 ccgatgtaga tagcggtcag gatggaaaag atgaatgcct gcagggtggc cacgatgccg
     4681 acctcgaaca cgtagaaggc gaaagccatc gggtaggtca ggacgccgag agcggcgata
     4741 ccgagcagga ctgccaggcc gccgcccatg aagagcagaa gcaggtggcc ggcgaggatg
     4801 ttggcgaaaa gtcggaccga cagcgagatc aggcggacga agtgggagac cacttcgatc
     4861 aggaagatcg ccgccttgcc gaaggggttc atgtcctcca ggccggaggg cagccagctg
     4921 gccaggtact tgatgaagcc cttggcccgg atgccttcga cgtggtacga gacccagacg
     4981 atcagggtca ggaccagcgg gatcgcgatg ttcgcggtgg ccgcatacag ggcaaaggcg
     5041 ggaatctcga gcccgaagat gttgatcgac tcatgcgtat tggtcggcag cggcaggtag
     5101 ccgagcatgt tcgagaagaa gatgaagaag aaaagcgagg cgaggaacgg gaaccacttc
     5161 ttcgcgagcc gctcgtcatc gatgttgttg ccggtgatgt cgttcttggt gacgtcgtag
     5221 gccacttcga tcgcggtctg gatccggttc ggcttctgct gcatcttgcg ggcgatccag
     5281 accatcacgg tgatggtcag catcgaagcg aggaacaggt agaggaccgc ccggttgatc
     5341 gagaggtcga tgcccccgag cttgatgttg atccagggat cgagcttgaa ctcgttctgc
     5401 ggcttgaact cctcgttctt gccggcggaa ccgaagatca ggccgaaggc gatcgcaacg
     5461 gcgagccaga tcccaatgcc gaagaaaacc ttggccttgg tgctgaagcc cgttttcgta
     5521 tcgggcatct gctcgtgggt tgcgacttcg ctcatcgggc agcttcctcc ggcttttcgt
     5581 ctttgccgag caggaaagcc agcccgcgtg cggcgaggtt cacggtaaag agaatgacca
     5641 gcagggccgc ggcgtacaga ccggcctcgc ggtcgacgat gcaggcgacc agcacgaccg
     5701 cggacatcag ccagggccgg gccagcgagg tcgaagcgac gaaccccatc gcccgggtgc
     5761 gctccccggc ggccagcttg tgcctggcct gccgttcggc ccagacctga atcagacgct
     5821 gggcggacca gacgacggcg gctaccgcgt agccggccag atcgaatccc gcaatgacga
     5881 aaacgggaag cgcgagaacc agaaccagaa ggtccagatt ggcgatcaag gagttgctgc
     5941 ggttacccat ttccccctcg agggatgtct ttgcggcata cgtcgtcaag ggacggcgac
     6001 tatatccaag aaaggcgggt cggcgcggtg cggtccggca aagtgacagg tgccactaac
     6061 cgtttatcca agccgaaaac cagacttcgt actttcttga tcaatcttct ccgttttgct
     6121 cgtcgcgcca gcggggtcgc ttgatgattt cgagcacaaa ggcgaggtaa acggttaccg
     6181 ccagcgcagc cagcaggacg accgccatga tcgtggtcca gagcgggtcg aaattgcccc
     6241 ggtcatcgct gtaggggacg aagcgcaggg ccagggcgag cagggccagg accagggtcc
     6301 agccgtagag atagagcagg gtgcgccgct gggagaaacc gatgttcgcc atccggtggt
     6361 ggaagtgcca gcggtccgcc tggaagatcg gctggcggta cttgagccgc ttggcgacca
     6421 cgaaggcggt gtcgaagagc ggcaccgcca ggatgaccag gggaaatacc atggcgatca
     6481 ccgcgttggt cttgagcgct ccctgcaccg aagccacggc gatcatgtac ccgagcaggt
     6541 tcgagccggt atcccccatg aaactggaag ccggcgggaa cccgtaccgc aggaatccga
     6601 tcgcaccgcc ggcggtcatg gcggcgagga cacccgcctc ataacgctga agcgagaccg
     6661 cgatgatcgc cattgccgcg gccgcgatcg cacagaagcc ggcggcgaga ccgtcgatgc
     6721 cgtcgatcag attgacgatg ttcatgacca gggcgatgcc cagcacggtc agcgggtagg
     6781 cgacccaccc cagctcgaag ccgccgaaga acggaacagt caggttggcc ggatagaccc
     6841 cgccgaccac cgggatcgtc gccgccccca gctggcccag cagcttgagc ccggccggca
     6901 ggtcgaacat gtcatccagt gccccgacca gggagacgac gcaggccccg agcaggatgc
     6961 tcagcgtctc cgtgctgatc ggcagccaga tccagcctgc cacctggatt ccgatcagga
     7021 tcgccagtcc ggacatgcgc ggggtcgggc tagtgtgctg gctgcgcggt cccggttcgt
     7081 cgatcgcatc gacccgccgg gccagccgct cggccggcct caccagaagg agagtgacca
     7141 gagccgccgt gaggaaggcc cagacggcat cgatcccttc aaccatgtcg ggaacaataa
     7201 atggcgatcc ggtcttcgcc ggatcgcaac agaaacccgc caacccaagg ctttgggttg
     7261 aagttatccg cacatggagc gaataacttc aacccaggtn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnc caaggctttg ggttgaagtt atccgcacat ggagcgaata
     7441 acttcaaccc aggtgccgac ctgctcctag gcgagggctt cgagcattcc ctggacgagg
     7501 aagtcagggt ctctcgggtt cttcgggccg gtttggtctt ttcgcttgac cagttcgagt
     7561 tgttcggcgc tttcgagaag cttcaggctg ccgtcggcga tcagggcctc gtcgacgccg
     7621 cccatccttc cggcgaaggt cgtatagacc ggggtgccga gggcgaccgc ctcgcggttc
     7681 atggtgccgc cggcgctgac caccaggtcg gcgtaggcaa tcaggctctg ggcatcgact
     7741 gcccggtcgg gaacgatcag gctggtcgaa ttcctggccc tggcccgctc ccgctggcct
     7801 tcggtgcggg gaatgattac cgcgctcacc ccgtccgcgc ccgccaggcg gtcgatcacc
     7861 ttctcgtaga ggggattgtc cgcgtggtag gcggcagtct cgggcggcgg ccgcacgatc
     7921 actaggaccc gctcaaggtc gatcccgagc gcttccggga cggtcggatc gggttcgaaa
     7981 ccggccaggt aatagtcctc cttgaggccg ggaaaacgga ccagcttgtt ctcccgggct
     8041 cccgccttga ccaggcgctc gaccgggatc gaatccggaa ccaggacccg cttcgccacc
     8101 cgccaggcga tcttccgctg caggccggcg aactcgtagt cctgcatctg ggccgaggga
     8161 atgaagaaag tctgcgagag gacggccaga tccacggaac cgtgggccac ggccaggtcg
     8221 ggccggaact tccagaccgg ccgggccagc cgggccgacc gcgaaccaag ggccagggcc
     8281 ttgccgagcc tgccggcgcc gccatgcctg ccgatcacca gatgcggaat gccgagccgc
     8341 tcgaggttgc cgaccgtctc cccgttctca cgggcggtga ccaggatctc gtcaccgcga
     8401 gcctggaaac gttcgatcac cggccggagc accacggggt gggcggcggc ggtgcagtcg
     8461 aaccagaccc tcatcggtgg atccccatcg gcttcggtcg ccaggggcag tcgctccgga
     8521 tcagaccagc cccgggtaca gggggtactt gtccatcagc gcctgtgaac ttgccgccag
     8581 cgaatcccgc tccgactcga agtcggggcc gagcgcggtg ctgatgatcc gggcgatctc
     8641 ggtcatgtcc tccggcgtga agccgcgggt cgagagggcc ggagttccga tcctgaggcc
     8701 ggaaggattc atcggcggcc gctcgtcaaa ggggatggcg ttgcggttga ccgtgatccc
     8761 gacctggtcg aggcgatcct ccccggtctg gccgtcgagc ccggtcgagg tcaggtcgac
     8821 cagcacgagg tgaacgtcgg tgccgtcggt caggacgtcg accccgccgt cgatcagccc
     8881 ggcggcaagc gccctggcgt tctcgacggt gcgacgctgg cgctcggcga agacgtcggt
     8941 cgaggcgatg cccagagcca cggccttcgc cgcgatcaca tgcatcagcg ggccgccctg
     9001 ctggccgggg aatacggcgc ggttcacgtc ggccttgagt tcttcggtgc agaggatcat
     9061 gccgctgcgg gggccgccga gagtcttgtg aatcgtggtc gtgaccacgt cggcgtacgg
     9121 caccggattc ggatgctcac cggccgcgac gaggccggcg aaatgtgcca tgtccaccat
     9181 cagcttcgcc ccgaccgaat cg
//
LOCUS       DAOQKN010000092         9107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_3643_length_9107_cov_5.942996, whole genome shotgun sequence.
ACCESSION   DAOQKN010000092 DAOQKN010000000
VERSION     DAOQKN010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 9107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9107
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3643_length_9107_cov_5.942996"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2342)
                     /locus_tag="PLJ64_07390"
     CDS             complement(<1..2342)
                     /locus_tag="PLJ64_07390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HNC93340.1"
                     /translation="MTTRRDAPAATVAVLFFTLIFGSLTWFAASSSEAATTPVPCAPG
                     SASCFAAAEGKAAPAATDVPTSPTTTPVQTPPDTGGGDQTGSDGGAVSTPGTGGVVPG
                     ETGGSGDGNGVTDPDGSTGEITPGGSEPDFVQQDPGLTGGAGPDSSVDTDPMTSDVLT
                     VPNFVIDQFEIPPFLLPIYQACGSEYGIPWYVLASINRTETSFGTSVATSSAGANGWM
                     AFMPASWAEWGVDANGDKVRDPYNPVDAICSAANYLKYFGYEQSPYDAIFAYNHADWY
                     VQIILRYAKIYSQIPPEVISALTGLTEGARFPVAAADASYEGEVSTEAAKKEGTASQD
                     IQSSSDRQTIAITASGGSPVIAVNDGTITDVNQETGTVVLEDAYGNRYSYAGLGSVST
                     VHPVPQPEPVSSLVSEESDSGSAPAGDASTGDGSSGQPDPEAEMGEEKAAAPTPAEKE
                     AFGSNPQAENQGSAEPTKQQQQDISEVLVDPDAAQAAAAAAATDPATSDDSNPAVKAA
                     DDRRAEQDSIRQVPAEADRNTEDSRGRVYASPLRPSNQHRATTDGQSIQNEIRQAEGV
                     GIEGKPGDYLVYDGSKSGIYRFDPETTELRELKKGSRVIAGTVLGRLAETPVASVNFS
                     IKPGGEDTPQIDPKPFLDGWKLLGETNIYSARGKDRFSQRLGVGGILLLSKSALQKRV
                     LNDPKMAFTQQCDRDYIAAGSIDRRLLATMEFITAHGYSLTISSMLCGRETSITTSGN
                     ISNHTHGGAMDIAIINGEVVTPGSQGPGSLTDQVARLVLTL"
     gene            2482..2823
                     /locus_tag="PLJ64_07395"
     CDS             2482..2823
                     /locus_tag="PLJ64_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93341.1"
                     /translation="MSAENDANEGIRDRVQSKGEKAAADLAQALIGSPAFGQAVSAAV
                     AARDRAAEAQRAAMGAMGVSSKDEEERLERRVRVLSDRLEETEDRLDAALDQIRELRA
                     ELKSSGSRKKT"
     gene            complement(2825..3526)
                     /locus_tag="PLJ64_07400"
     CDS             complement(2825..3526)
                     /locus_tag="PLJ64_07400"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93342.1"
                     /translation="MPVKMNPIEPAAGDAILVGDPRRAFALAQELTAQPKMSHQARGL
                     WGYKGLTDDGLRLTIQSTGVGGPSAVPVIGDLASQGVSRMIRLGTCLAIEPGLAAGTL
                     LLVSRAVVADGTSRVLGAGQDFVRPDQGLLGALGGLGREATISSHDLVQRYEPEGTGR
                     SDPGPALARDLQTAATLAMCRRLEIPAAALLIVAEDTAGNRLDEEAMWDLFRSAGKAV
                     LSRLARLEPNPQVEV"
     gene            3569..3880
                     /locus_tag="PLJ64_07405"
     CDS             3569..3880
                     /locus_tag="PLJ64_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93343.1"
                     /translation="MAESGSEKSGGRGVPDGLKEAIEGAFAATGKTRAQAQDLTEKTR
                     NRAQGLVDEVTRRGSDARGTLDGLRLVSREEMRQLEERIDEISSRLAELERKSKAQPE
                     G"
     gene            3932..4948
                     /locus_tag="PLJ64_07410"
     CDS             3932..4948
                     /locus_tag="PLJ64_07410"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNC93344.1"
                     /translation="MSATKRIVIAGVSTRWGSELARTLQRRPGVGEIIGIDSAAPTSD
                     LGRTEFVEADIRNPVISRILPSLEPDVVVHCGIVWYPEKGRPSRALHDINVIGTLQLL
                     AACEKTSGLQALIARGSAAIYGSAPGAPAFFTEDMARRLPLVTRFQRDIGELEGYFDN
                     FARRRPEITCCMLRFGVEVGPGLDTPFNRYLNLPVVPTRMGFDPRLQLLHVDDATGAL
                     AAATLNPVRGAVNVAPDGAISLSRLLRLCGKSAVGVPPVLVGVINRRIGNYLGSADLY
                     RDGELLLRYGRGCDNRRLREEVGFELAFDSEGTARDFARTSQGIQALFPSPHPGSPVR
                     SVSR"
     gene            4945..5820
                     /locus_tag="PLJ64_07415"
     CDS             4945..5820
                     /locus_tag="PLJ64_07415"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNC93345.1"
                     /translation="MNRPAAPTPEAVLEWAGTVQSTSDLILRRLAGEYHEDAWGFDEE
                     FTEAVFPLFEFLYSTWWRVRVEGAANVPGHGRALLVANHSGAIFPFDGAMIATAIQKE
                     HPLPRWVKAMVLNWAFDLPFVSYILRRLGGVAANPANLGRLLEEDQPVMVFPEGQKGF
                     GKSFADRYRLQRFGRGGFVEVALETGSPIVPVAVVGAEEIYPKLGESKPLARAIGSPY
                     VPVTPTFPWLGPLGLLPLPSRWRIEFLEPIDLSGYGPGSSEDRSLVFDLSEQVREQIQ
                     EALYRGLVKRGPAFL"
     gene            6115..7593
                     /locus_tag="PLJ64_07420"
     CDS             6115..7593
                     /locus_tag="PLJ64_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNC93346.1"
                     /translation="MSVKRIGIASLLAMFAALVVPGMASAAPVTQELFDAKSAELNGT
                     FIALACVLVLLMQCGFMFLEIGFSRMKNAGAGVMKIFVNFSVCTLAFWAVGAAIIGYG
                     NDFIGTHGFFYHFGSMVDDGSGTGTLVAVDSSAVMYMLYSFAFCAVSLAIVWGTTLER
                     IKFAPYIIYAAVFGAVIYPMVGHSVWGGGLLTDIGGKSVMDFAGSSVVHLTGAVGGFV
                     ALLLLGARKGKYAADGSPRAIPGHSMPLVGLGIIILWIGWFGFNGGSTLGTDLSFFGE
                     VMWNTQIAAAAGVIGAMLFTYMKTKSLDVGMAGNGAIAGLVAITAPCGFVETWTAPII
                     GFIAGGVVVIGVLTIEKYLDDPIGALSAHGLAGIWGTLAVGIFASPRLTISAPGIWYG
                     IFGDADFGATLGQLGVQALAVVATFTFVFAASYLVFGIIKATIGLRVTEAEEDAGLDI
                     ASHGMYGYPEAFIPQPELPVGEYTPSMAGTPVASSAESPSQA"
     gene            7609..7965
                     /locus_tag="PLJ64_07425"
     CDS             7609..7965
                     /locus_tag="PLJ64_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNC93347.1"
                     /translation="MKKIEAFIRHEAFEPIRTDLLEKGIPSMSILEAKGSGRQKGIVE
                     TYRGSTVTVNVRPKLKIEIVVADSEKDLVVETILRHARSGEIGDGKIFVIPVEESIRI
                     RTGERGDEVTQVHLDD"
     gene            8083..>9107
                     /locus_tag="PLJ64_07430"
     CDS             8083..>9107
                     /locus_tag="PLJ64_07430"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HNC93348.1"
                     /translation="MADRSRIGVLVTGGTVGSRSEPLSGREKLVRLSESDRQLDVFSG
                     PNVTAVLEKAGISPVLRQPVNMLSENMTPEHWVIMGREIERLIEEEDPDGILVLHGTD
                     TMSFTSTALSFMLAGLKVPVVLTGSNRPPNEPDTDADTNIADALIALQHLSPGVYLSF
                     AGRPNAKSRVHDGVSVRKARGSGPAFISAGRVHVAEVSWGTFSWTLKSRANPDEVTEG
                     PDSSRIAADDRVMRIDLHPGINLESMADMLIRDGFRGAVMTLYPALTGPFEPERSSVP
                     RFAHRLAGHGIVLALTMNAQPVGVQNSYESKLLLEETGAVLLPVIPELALVKLMWALG
                     WSSDPDEV"
BASE COUNT         1469 a         2944 c         3056 g         1638 t
ORIGIN      
        1 agcgtcagaa ccagacgggc cacctggtcg gtgagtgaac cggggccctg gctgcccgga
       61 gtgacgacct cgccgttgat gatcgcgatg tccatcgcgc caccgtgggt gtggttggaa
      121 atgttgcccg aggtggtgat cgaggtttcg cggccacaga gcatcgaact gatggtcagc
      181 gagtagccat gggcggtgat gaattccatg gtcgccagca ggcggcggtc gatcgagccg
      241 gcggcaatgt aatcgcggtc gcattgctgg gtgaatgcca tcttcgggtc gttcagcacc
      301 cgcttctgga gggccgactt cgaaagcagg aggatgccgc cgacaccgag ccgttgcgag
      361 aagcggtcct tgccgcgggc gctgtagata ttggtttcgc cgagcagctt ccagccgtcg
      421 aggaagggct tgggatcgat ctgcggggtg tcttcgccgc ccggcttgat cgagaagttg
      481 accgaggcga ccggcgtttc ggccaggcgg ccgagcacgg tgccggcgat cacccgcgag
      541 cccttcttca gttcccgaag ttcggtggtt tcagggtcga accggtagat gccggacttg
      601 ctgccgtcat agaccaggta gtcgccgggc ttgccctcga ttccgacgcc ttcggcctgg
      661 cggatctcgt tctggatcga ctggccgtcg gtcgtcgcgc ggtgctggtt gctgggacga
      721 agcgggctgg cgtagacgcg gccgcggctg tcctcggtat tgcggtcagc ctcggccggc
      781 acctggcgga tgctgtcctg ctccgcgcgc cggtcgtcgg cggctttgac cgcggggttg
      841 gaatcgtcgc tcgtcgcggg gtcggtcgcg gctgcggcgg cggccgcctg ggcggcgtcg
      901 ggatcgacca gcacctcgct gatgtcctgc tgctgctgtt tggtcggttc ggccgaaccc
      961 tgattctcag cctgagggtt agatccgaag gcttcctttt cggccggggt gggagcggct
     1021 gccttttcct cgcccatctc cgcttcgggg tcgggctgcc cggatgagcc gtcaccggtg
     1081 gaggcatctc cggccggggc cgatcccgaa tccgattctt ccgaaaccag cgaggagacc
     1141 ggttcgggct ggggtaccgg gtggacggtt gaaaccgaac cgaggccggc gtaggagtac
     1201 cggttgccgt aggcgtcctc gagcacgacc gtgccggttt cctgattgac gtcggtgatc
     1261 gtgccgtcgt tgaccgcgat cacgggcgag ccacccgagg cggtgatcgc gatggtctgg
     1321 cgatcggacg aggactggat gtcctgggag gcggtgcctt ccttcttcgc ggcttcggtc
     1381 gatacctcgc cttcgtagga agcgtcagcg gcggcgaccg ggaagcgggc cccttcggtc
     1441 agaccggtca gggcgctgat cacctcgggc gggatctgcg agtaaatctt cgcgtaccgc
     1501 aggatgatct ggacgtacca gtcggcgtgg ttgtaggcga agatcgcgtc gtacgggctc
     1561 tgctcgtagc cgaagtactt gaggtagttg gcggccgagc agattgcgtc gaccgggttg
     1621 taggggtccc ggaccttgtc cccgttggcg tccacgcccc actcggccca ggaggcgggc
     1681 atgaaggcca tccagccatt tgctccggcc gaggaggtgg ccacgctcgt gccgaagctg
     1741 gtctcggtcc ggttgatcga ggcgagcacg taccagggga ttccgtactc gctgccgcag
     1801 gcctggtaga tcgggaggag gaagggcgga atctcgaact ggtcgatcac gaagttggga
     1861 accgtgagga catccgaggt catcgggtcg gtgtcaacgc tgctgtcggg cccggcgccg
     1921 ccggtcaggc cggggtcctg ctgtacgaag tcgggttcgc tgccgccggg ggtgatctcg
     1981 ccggtgcttc cgtcggggtc ggtcaccccg ttgccgtcgc cgctgccgcc ggtctcgccg
     2041 ggcaccactc cgccggtgcc gggtgtgctc acggcgccgc cgtcgctgcc cgtctgatca
     2101 ccgccgccgg tgtccggggg ggtctggacc ggtgtggtcg tgggggaggt cggaacgtcg
     2161 gtggcggccg gtgccgcctt gccctcggcc gccgcgaagc agctggccga tcccggagcg
     2221 cagggaaccg gagtcgtggc ggcttcggaa gaggacgccg caaaccaagt cagggagccg
     2281 aaaatgagcg tgaaaaagag gaccgctact gtggcggccg gcgcatcgcg gcgtgtagtc
     2341 attgaaccct ggtccacctt tggcttccca actaaaacag acacggccga cgccctcccg
     2401 tgacgcggca gaggaaccgt atcgcaacgg ctttcgccgg tacctcgaca cttcctgcga
     2461 agcgcccttt aagatcgtgt catgtcagct gaaaacgatg ccaacgaggg aatcagggac
     2521 cgggtccagt cgaagggcga gaaggccgcg gccgacctcg cccaggcgct gatcggaagt
     2581 cccgcattcg gtcaggcggt ctccgctgcg gtggccgcga gggatcgcgc cgccgaagca
     2641 cagcgggcgg cgatgggtgc catgggcgtc tcttcgaaag acgaggaaga gcggctcgaa
     2701 cggcgggtcc gggtcctttc tgaccgtctc gaagagaccg aagaccggct cgacgcggca
     2761 ctcgaccaga ttcgggagct gcgagcggag ctgaaatcgt caggaagccg gaaaaagacc
     2821 tgacctaaac ctcaacttga gggttaggtt ctagccttgc caatcggctg agcaccgcct
     2881 tgccggcgct ccggaacaga tcccacatcg cttcctcgtc gagccggttc cccgccgtgt
     2941 cctctgccac gatcagcagc gccgcggccg ggatttccag ccggcggcac atcgcaagcg
     3001 tggcggcggt ctgcaggtcc ctggcgagcg ccggtcccgg atcggaccgg ccggttccct
     3061 ccggttcgta acgctgaacc aggtcgtggc tcgagatcgt cgcttcgcgc ccgaggccgc
     3121 cgagcgcgcc gagcagacct tgatcgggcc gcacgaagtc ctggccggcg ccgagcaccc
     3181 gggaagtccc gtcggcaacc acggctcgac tcaccaggag cagcgttccg gcggccagtc
     3241 cgggctcgat cgcaaggcag gtgccgagcc tgatcatccg gctcaccccc tgggaagcga
     3301 ggtcgccgat caccggaacg gcgctcgggc cgccgacgcc ggttgactgg atggtgagcc
     3361 ggaggccgtc atcggtcagg cctttgtagc cccaaagccc gcgggcctgg tggctcatct
     3421 tcggctgtgc ggtcaactcc tgggcgaggg cgaaggcgcg acgggggtcg ccgaccagga
     3481 tcgcgtcacc ggcggcgggc tcgatcgggt tcatctttac gggcatcgga ttctccggcg
     3541 gggcggtctt tcgtcgtaaa ctagccgcat ggctgaaagc ggatcggaga agtcaggcgg
     3601 ccgcggcgtt cccgacggtc tcaaagaagc gatcgagggt gcgttcgccg cgaccggcaa
     3661 gacccgggcc caggcccagg atctcaccga gaagacgaga aaccgggccc aggggctggt
     3721 cgacgaggtg accaggcgcg gatccgatgc ccgggggacg ctcgacgggc ttcgcctggt
     3781 cagccgcgag gagatgcgcc agctggagga gagaatcgac gagatttcca gtcggctagc
     3841 cgaattggag cgtaagtcaa aggctcaacc tgagggttag agttagtcga tttggccccg
     3901 tttttggcca gatcccggtc cggagttcaa attgagtgcg acaaaacgga tagtcatcgc
     3961 cggagtttca acccgctggg ggtcggaact ggcgcggacg ctgcagcgcc ggcccggggt
     4021 cggcgagatc atcggcatcg attcagccgc gcccacttcc gatctcggcc ggaccgagtt
     4081 cgtcgaggcg gacatccgca acccggtcat atcccgcatc cttccctcgc tggaaccgga
     4141 cgtggtggtt cattgcggga tcgtctggta cccggaaaag ggccggccct ccagggcact
     4201 ccatgacatc aacgtgatcg gcaccctgca gctgctcgcc gcgtgcgaaa agacttccgg
     4261 tctccaggcc ctgatcgccc ggggttcagc cgcgatctac ggttccgccc ccggcgcccc
     4321 ggccttcttc accgaggaca tggcccgccg cctgcccctg gtcacccgct tccagcgcga
     4381 catcggcgag ctcgagggct atttcgacaa tttcgccaga aggcgccccg agatcacctg
     4441 ctgcatgctc cgcttcgggg tcgaggttgg ccccggcctc gacaccccct tcaaccgtta
     4501 cctgaacctg ccggtggtgc ccacgcgcat gggtttcgac ccccggctgc agctgcttca
     4561 cgtcgacgat gcgactgggg cgctggccgc ggcgaccctg aacccggtgc ggggagcggt
     4621 caacgtcgcc cccgacggcg cgatctcgct cagccggctg ctgcggcttt gcggaaagag
     4681 cgcggtcggc gtccccccgg tattggtcgg cgtgatcaac cgccggatcg gcaactacct
     4741 cggcagcgcc gatctctacc gcgacggcga gctcctgctc cgttacggcc gcggctgcga
     4801 caaccgcagg ctgcgggagg aggtcgggtt cgagctcgcc ttcgactcgg agggaacagc
     4861 ccgcgacttc gcccgcacct cgcagggcat ccaggccctc tttccgtcac cccatccggg
     4921 ttccccggtc aggagcgttt ccaggtgaac cgccccgccg ccccgacccc ggaggccgtg
     4981 ctcgagtggg ccggaacggt ccagtccacc tcggatctga tcctccgccg gctggccggc
     5041 gagtaccacg aggatgcctg gggcttcgac gaagagttca ccgaagcggt cttcccgctc
     5101 ttcgaattcc tctactcgac ctggtggcgg gtccgggtcg aaggggcggc caacgtgccg
     5161 gggcacggcc gggccctgct ggtcgccaac cattccggcg ccatcttccc ctttgacggc
     5221 gcgatgatcg ccaccgcgat ccagaaggag caccccttgc cccgctgggt caaggcgatg
     5281 gtgctcaact gggccttcga cctgcccttt gtcagttaca tcctgcgccg gctcggcggg
     5341 gtggccgcca acccggcaaa cctcggacgg ctgctcgagg aggatcagcc ggtgatggtc
     5401 ttccccgaag gccagaaggg cttcggaaag tcattcgccg accgttaccg gctgcagcgc
     5461 ttcggccgcg gcgggttcgt cgaggttgcg ctcgagaccg ggtcgccgat cgtcccggtc
     5521 gcggtggtcg gggccgagga gatctatccg aaactcggcg agtcaaagcc gctggcgcga
     5581 gcgatcggtt ctccctacgt gccggttacc ccgaccttcc cctggctggg accgctcggc
     5641 ctgctgcctt tgccctcgcg ctggcggatc gagttcctcg agccgatcga cctctccggg
     5701 tacggaccgg gttcgagcga ggaccgctcg ctcgtattcg atctctccga gcaggtgcgg
     5761 gagcagatcc aggaagccct gtatcgcggc ctggtcaaac gcggccccgc attcctctag
     5821 cccgcggccg tgaagtccgg gggccggcgg cccagttgcc tggccgggtg tcccggcgga
     5881 caaaacggcc gccgatccag tcgcccgagt ccgctcacag acccgaatct caactacgac
     5941 tcggctctgt agagccattt accttcgatt taacaatcga acaaccggga tggggtcatt
     6001 tcctccgagt ggccaaaaag cgctccccct cccgtttcta aattggcccg gtcagctaaa
     6061 cgctatttgc aaccggtggg tctgggcccg gcggaaacag gaggaatgac tcgtatgagt
     6121 gtcaagagaa tcggcatagc ctcgcttctg gcgatgtttg ccgccctggt ggtgccaggg
     6181 atggcgagtg cggcgccggt cacacaggaa ctgttcgacg ccaagtcggc cgagttgaac
     6241 ggaaccttca tcgcgctcgc ctgcgtgctc gtgctgctca tgcagtgcgg cttcatgttc
     6301 ctggagatcg gtttctcccg aatgaagaac gcaggcgccg gagtgatgaa gatcttcgtc
     6361 aacttctcgg tctgcaccct cgccttctgg gcggtcgggg ccgcaatcat cggttacggc
     6421 aacgacttca tcggaaccca cgggttcttc taccacttcg gttcgatggt cgatgacggc
     6481 agcggcacag gcacgctggt cgccgtcgat tcgagcgcgg tcatgtacat gctgtacagc
     6541 ttcgcgttct gcgcggtctc gctcgccatc gtctggggca ccaccctgga gcgcatcaag
     6601 ttcgctccgt acatcatcta cgccgcagtc ttcggagccg tgatctaccc gatggtcggt
     6661 cactcggtct ggggcggcgg cctgctcacc gacatcggtg gcaagtcggt gatggacttc
     6721 gccggttcct cggtggtcca cctcaccgga gcggtcggcg gattcgtcgc gttgctcctg
     6781 ctgggtgcca gaaagggcaa gtacgcggcc gacggatcac cccgtgcgat ccccggacac
     6841 tcgatgccgc tggtcggcct cgggatcatc atcctgtgga tcggctggtt cggattcaac
     6901 ggcggctcga ccctgggcac cgatctctcc ttcttcggcg aggtcatgtg gaacacccag
     6961 atcgctgctg cggcaggcgt gatcggcgcg atgctcttca cctacatgaa gaccaagtcg
     7021 cttgacgtcg gcatggccgg caacggcgcg atcgccggcc tcgtcgcgat caccgcgccc
     7081 tgcggtttcg tcgagacctg gacggccccg atcatcggct tcatcgccgg tggcgtcgtg
     7141 gtcatcggcg tcctgacgat cgagaagtac ctcgacgatc cgatcggtgc cctctcggcc
     7201 cacggtctgg ccggcatctg gggaaccctc gcggtcggca tcttcgcctc gccgcgactg
     7261 accatctccg cgccgggcat ctggtacgga atcttcggtg acgccgactt cggcgcgacc
     7321 ctgggtcagc tcggggttca ggcgctggcc gtcgttgcga cgttcacatt cgtgttcgcc
     7381 gcttcgtacc ttgtgttcgg aatcatcaag gcaaccatcg gcctccgtgt cacggaagcg
     7441 gaagaggatg ccggtctcga catcgcctcg cacggcatgt acgggtaccc ggaagccttc
     7501 atcccgcagc cggagctgcc ggttggtgaa tacaccccgt cgatggcagg gacaccggtc
     7561 gcatcgtcgg ccgagagtcc cagccaggcc taggaaggag atgctcagat gaaaaagatc
     7621 gaagcattca ttcgccacga ggcgttcgag ccgatccgga cggacctcct cgaaaaagga
     7681 atcccgtcaa tgtcgattct cgaagccaag ggctcgggac ggcagaaggg gatcgtcgag
     7741 acctaccggg gatcgacagt cacggtcaac gtccggccga agctcaagat cgagatcgtc
     7801 gtggccgatt ccgaaaaaga cctcgtcgtc gaaacgatcc tgcgtcacgc tcgatccgga
     7861 gagatcggag acggcaagat cttcgtaatc ccggtcgagg agtcgatcag gatcaggacc
     7921 ggcgagcgag gagacgaagt aacccaggtc catctcgacg actagcgatg ggctgacacg
     7981 gatcgggcgg cggggcctcg tgccccgccg cccttgtccg ttgtcgggac tgatttgact
     8041 acagccgcca accgcattcc ggccgccaga tacgctggct ccgtggccga ccggagcaga
     8101 atcggagtcc tggtgaccgg gggtaccgtg ggctcccgct cggagcccct ttcgggccgg
     8161 gaaaagctgg tcaggctcag cgagtccgac cggcagcttg atgtgttctc cggtcccaac
     8221 gtgaccgccg ttctcgaaaa ggcgggcatt tcaccggttc tccggcagcc ggtcaacatg
     8281 ttgtccgaga acatgacgcc cgaacactgg gtgatcatgg gccgggagat cgagcgcctg
     8341 atcgaggaag aagatccgga cgggatcctc gtcctgcatg gcaccgacac gatgtccttc
     8401 acctcgacgg cactctcatt catgctcgcc ggcctcaagg tcccggtggt gctgaccggg
     8461 tcaaaccggc cgccgaacga gccggatacc gacgccgaca ccaacatcgc ggacgccctg
     8521 atcgcgcttc agcatctctc gcccggggtc tacctctcct ttgccggccg gccgaatgca
     8581 aagagccggg tgcacgacgg ggtgtccgtc cgcaaggcca gaggctccgg gcccgccttc
     8641 atctcggcgg gaagggtcca cgttgccgag gtcagctggg gaaccttcag ctggaccctg
     8701 aagagccgtg cgaacccgga cgaggtcacc gagggtccag attcttcccg gatcgccgcc
     8761 gacgaccgcg tgatgagaat cgatctccac ccggggatca atctcgaatc gatggccgac
     8821 atgttgatcc gcgacggctt ccgcggcgcg gtgatgaccc tttacccggc tctgaccggg
     8881 cctttcgaac cggaacgctc ctcggtgccg cggtttgccc atcggcttgc cgggcacggg
     8941 atcgtgctcg ccctgaccat gaacgcccag ccggtcgggg tccagaattc ctacgaatcc
     9001 aaacttctgc tcgaggagac cggggccgtg cttctgccgg tgatccccga gctcgccctg
     9061 gtcaagctga tgtgggctct cggctggagc agtgacccgg acgaggt
//
LOCUS       DAOQKN010000093         8831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_3805_length_8831_cov_5.892662, whole genome shotgun sequence.
ACCESSION   DAOQKN010000093 DAOQKN010000000
VERSION     DAOQKN010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 8831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8831
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3805_length_8831_cov_5.892662"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="PLJ64_07435"
     CDS             <1..440
                     /locus_tag="PLJ64_07435"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNC93349.1"
                     /translation="RLALAAEPETEVLTDLARRVSPRIGRTGRDALTDARRQLDDYFG
                     GELRTFDLPLDWRLTRGFRREGLHETAAIPYGATASYAQMARQAGSPRAVRAAGSALA
                     TNPLPIVVPCHRVLRSDGAVGSYLGGSPMKESLLKMERERTAA"
     gene            437..1294
                     /locus_tag="PLJ64_07440"
     CDS             437..1294
                     /locus_tag="PLJ64_07440"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HNC93350.1"
                     /translation="MSWSLRVDANGPLDTAAIFSTLASHEIAGLTETRPDRLTHRRVL
                     EFEGRPVILTVWFDGSGVTIEGETGDGPWPGVEPLVRHWFDLDTDIEAVDRHLGVHPQ
                     FKNQVRARPGIRVTRYPELAESAILTVLGQQISLAAARTFAGRLVAAYGEEVDGLFAF
                     PDPARIAEEPVERLRSTIGLTGARARTISGVATLFADRRPAAGSGSDSGLLEGLNEIP
                     GIGPWTISCVALRGLDSADELPASDAVLRRALGKASLAETLEMAEAWRPFRSYATVRL
                     WAESVPLAG"
     gene            complement(1306..1623)
                     /locus_tag="PLJ64_07445"
     CDS             complement(1306..1623)
                     /locus_tag="PLJ64_07445"
                     /inference="COORDINATES: protein motif:HMM:NF019139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HNC93351.1"
                     /translation="MKKSVAIGIAGVTAVVVAAWRQVQRDNTLATDAGRAPVPARPPA
                     VKNDAAPSTPAPSSSPDALFTPSVSASSTKAELYEVATELGIEGRSKMNKTELLEAIR
                     TAS"
     gene            1724..2404
                     /locus_tag="PLJ64_07450"
     CDS             1724..2404
                     /locus_tag="PLJ64_07450"
                     /inference="COORDINATES: protein motif:HMM:NF024929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HNC93352.1"
                     /translation="MKISRSSLIAAAVLVTMAVAAPAGAPADPAPRAGEGFQGRIFRI
                     DRDLRERMVGSSWHRGCPVRLADLRLLRLSYRDFDGDVRRGYLVVHETAAPRMVKAFG
                     RLFNAGYRIRRMRLVDAYGADDHRSMNADNTSAFNCREVAGQPGVWSRHAYGRAIDIN
                     PVENPYVSGSHVSPARGRPFADRYPRRKGMISAGGRVVRIFAAIGWGWGGDWAGAKDY
                     QHFSASGD"
     gene            2488..3075
                     /locus_tag="PLJ64_07455"
     CDS             2488..3075
                     /locus_tag="PLJ64_07455"
                     /inference="COORDINATES: protein motif:HMM:NF020905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1989 domain-containing protein"
                     /protein_id="HNC93353.1"
                     /translation="MAMAEITTEVPHGTARRFDLRTGDLIRIETLDGAQGGDFSFPGF
                     DQALTRNKLGWKRFGRPWLVYDAQAGDSLFDVDGEATFELIALEGEGVADIMYPGCWD
                     EVYEDRRPGCQDLISAALTIPRRDLAGMLSFFIGYSADNSAYRGLAGIDLKPGDHLTF
                     RALRDVSCAVSACPDRDIPGWRVGALFVSVTEAGK"
     gene            3072..3935
                     /locus_tag="PLJ64_07460"
     CDS             3072..3935
                     /locus_tag="PLJ64_07460"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNC93354.1"
                     /translation="MNGAGSFRLAVAQPAIDREAPESSRVAEAGAMIREAGRGEADLI
                     LFPESFPGPITAGLEYDAAPDLGAACRDSGVAACWGRVEPIGEGRFVTVVRLTDRTGG
                     EAGRYVRSHPATGDVHPVLSGVPMAPGPEPGIFELDGLRLGIVVCSELWNPELCRVLA
                     LRGAEVLLAPAGGGFHSVAENWQLMARARAIENEMFVALTQQLFDGESGSALIAGPEG
                     VLSAGTGPGLVIADLDLERARWLRSQDDSMQEPKPFSSLPGLLRARRPELYGELGAPQ
                     EGLYDFEAASS"
     gene            complement(3908..5410)
                     /locus_tag="PLJ64_07465"
     CDS             complement(3908..5410)
                     /locus_tag="PLJ64_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HNC93355.1"
                     /translation="MTASITSRPGESSGNRSAIWTLIITGLALFMTSLDNLVVGVALP
                     SIRADLGGTIESLEWTVNAYTLAFAVLLITGAALGDRFGRRRMFAIGVGLFTAASAAS
                     ALAPTLDALVAARALQGAGAAIVVPLTLTLVSGAFPAEKRGAALGIWGGISGLGVALG
                     PLFGGAVVEGISWHWIFWLNVPVGLALIPLALRRLPESTGPDKALDLPGLGLVGTGLF
                     GLTFGIIRGESLGWTSTTVLASLVTGALLIGAFIVWELRAREPMLPMRFFRSRGFSAT
                     NGLSFAMFFGTFGAIFLLSQFFQVAQGLGPLDAGIRTLPWTLMPMFVAPLAGFLSDRI
                     GTRPLIFTGLVLQAGGLAWITAISSPVTAFGDFVTPFMMAGTGMALVFPTAAATVVAS
                     VREEETGKASGATNAIREIGGVMGVAVLSSIFAANGGFTSPQAFTDGVVAAMPVAVAV
                     LAVGAVLALLIPGKDAVGDDAAKTDRDAARTGEPRPSLRPEPQLLAASKS"
     gene            complement(5624..6259)
                     /locus_tag="PLJ64_07470"
     CDS             complement(5624..6259)
                     /locus_tag="PLJ64_07470"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC93356.1"
                     /translation="MATTSDKIAASPGTGTARTRSTGSRSLSTAEERREELITAAIPV
                     FAEKGYHAAPTTEIAKKAGISQAYLFRLFPTKEELFVAAVERSTGRMVETLAAVASEA
                     EKNGIEPGDAMGLAYGDLVSSDRELLLMQLHAQAVSPAIPAVREAMRKCFANLYALGS
                     ERTGGNGEEMKNWFAHGMLINVMTAINAEEVDEPWAVALTDFEGDCFPRES"
     gene            complement(6341..6574)
                     /locus_tag="PLJ64_07475"
     CDS             complement(6341..6574)
                     /locus_tag="PLJ64_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93357.1"
                     /translation="MGDLVRRKWHLMLIGLIAGAVIVGLVIGPLVVKVFGTVAGVYLI
                     LFLILRRRKGPDGKINPWYASNGRQKGGGIFPF"
     gene            complement(6804..>8831)
                     /locus_tag="PLJ64_07480"
     CDS             complement(6804..>8831)
                     /locus_tag="PLJ64_07480"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HNC93358.1"
                     /translation="SDEFMQAVADDAEWNLLARTDGSVTKTMSARDLMNQIADAAWQC
                     ADPGVQYDTIINKWHTCPNSGRINASNPCSEYMHVDDSACNLASINLMKFRREDGSFD
                     VDGFCAVVDTVFLAQEIIVSPSSYPTEEIGKNARAFRQLGLGYANLGALLMSDGMPYD
                     SDEGRNVAAAITSLMTGRAYRRSAEVAAAMGPYDAYELNREPHNNVMRMHRDAAYEIS
                     GEGVHEELLNAAREEWDVAVATGDEHGYRNAQATVLAPTGTISFLMDCDTTGIEPDFS
                     LVKFKELVGGGNMTIVNKSVPLALRTLEYSESEISQIEAYINEHGTIIGAPALKEEHL
                     PVFDVAVGERAISHTGHIDMMAATQPFLSGAISKTVNMPNSATVEDIADAYTRGWEGG
                     LKALAIYRDGSKTAQALRTDAQKDKEKEAEAEAAPNEPVRHRMPKTRDSVTHKFSVGG
                     HEGYITAGKYTDGSVGEIFLTDIGKEGSTMRGMMNAFATAISLGLQYGVPLEVFVSKF
                     SYMRFDPEGITGNPDIPFAKSMPDYIMRWLASQFIDDPDFLEEQGIMTQEVRNRKEAE
                     NAGINLANGSGNGSPAGNGGNGNGNGGNGNGGSAPSASTESAAPVADTGGSAVPAGTA
                     LTDDVPVVPARLHFTPGAAELGPACDQCGGMMQRTGACYTCSSCGNNTGCG"
BASE COUNT         1402 a         2875 c         3044 g         1510 t
ORIGIN      
        1 ggcgcctcgc cctcgccgcg gaaccggaaa ccgaagttct cacggacctc gcccggcggg
       61 tttcgcccag gatcggacgc accggtcgcg atgccctgac cgacgcccgc cggcaactcg
      121 acgactattt cggaggagag ctcagaacct tcgacctgcc gctcgactgg cggttgaccc
      181 ggggcttccg gcgcgagggg ctccacgaaa cggcagcgat tccctacggt gccacggcaa
      241 gctatgccca gatggccagg caggctggaa gcccgcgggc ggtcagggcg gcaggctcgg
      301 ccctcgccac aaaccccctg ccgatcgtcg ttccctgcca tcgcgtgctg cgctcggacg
      361 gggcggtcgg ttcctatctc ggtggcagcc cgatgaaaga gtcgctgctg aagatggagc
      421 gcgaacggac cgccgcttga gctggtcgct tcgagtcgac gccaacgggc cactggatac
      481 cgccgccatc ttctcgaccc tcgcgagtca cgagatcgcc ggcctgaccg agaccagacc
      541 ggaccggctc acccaccgcc gggtcctgga attcgagggc aggcccgtga tcctgaccgt
      601 ctggttcgat ggttcgggag tcacgatcga aggggaaacc ggcgacgggc cgtggcccgg
      661 ggtcgagccg ctggtcaggc actggtttga tctcgacacc gatatcgaag ccgtcgatcg
      721 gcatctcggc gttcatccgc aattcaagaa ccaggtccgg gctcgtccgg ggatccgggt
      781 gacccggtat ccggagcttg ccgaatcggc gatcctgacc gtgctcggcc agcagatttc
      841 gctggccgca gcgcggacct tcgccggccg gctggtcgcc gcctacgggg aggaagtcga
      901 cgggctgttc gcctttcccg atcccgcccg aatcgcggaa gagccggtcg agcggctgag
      961 gtcaacgatc ggactcacgg gagccagggc gagaacgatc agcggggtcg cgaccctgtt
     1021 cgccgaccgt cggcccgccg ccggatccgg cagcgacagc ggactgctcg aggggttgaa
     1081 cgaaatcccc ggaatcgggc cgtggacgat ctcctgtgtc gccctgcgcg ggctcgactc
     1141 ggccgacgag ctgccggcgt ccgacgcagt cctccgtcgc gcgctcggga aggcgtccct
     1201 ggcggaaacc ctggaaatgg cggaagcctg gcgaccgttt cgcagttacg cgacggtccg
     1261 gctctgggct gaatcagttc cgcttgccgg ctaggcggcg gaccctcagg aagcggtgcg
     1321 gatcgcttcg agcagctcgg tcttgttcat cttcgagcgg ccctcgatgc ccagttccgt
     1381 tgcgacctcg taaagctcgg ccttggtcga gctggcgctg accgacgggg taaagagggc
     1441 gtcgggggag ctgctcgggg ccggggtgga cggagccgca tcgtttttga ccgccggggg
     1501 ccgggcgggc accggagctc gaccggcatc ggtggcaaga gtgttgtccc gctggacctg
     1561 acgccaggcg gcgacaacga ccgcggtcac gccggcaatg ccgatcgcga cggacttctt
     1621 catgacttcg accatttgag actggaaggg aagcaattga tcacaggcag gactctaccg
     1681 acgaagcggt ttggacttct ggacgattgt ctagtctcga atcgtgaaga tttcccgatc
     1741 gagcctgatc gctgcggccg tcctggtgac gatggccgtt gcggcgcctg cgggtgcccc
     1801 cgccgatccg gcgccccggg cgggggaagg gttccagggc aggatcttca ggatcgaccg
     1861 ggatctgaga gaacgcatgg tcggttcctc ctggcatcgg ggatgcccgg tccggcttgc
     1921 cgacctgcgg ctgctcaggc tgagctaccg ggatttcgac ggcgacgtcc ggcgtggata
     1981 cctggtcgtc cacgagacgg ccgcgccccg catggtcaag gcttttggcc gtctcttcaa
     2041 cgccggctac cggatccgtc gcatgcgcct ggtcgatgcc tacggcgccg atgaccaccg
     2101 ctcgatgaac gccgacaaca cctcggcttt caactgccgc gaggtcgccg gccagcccgg
     2161 ggtctggtcc cggcatgcct acggccgggc gatcgacatc aatccggtcg agaacccgta
     2221 tgtgtcaggg agccacgttt ctccggccag gggccggcct ttcgctgacc gctacccccg
     2281 ccgcaaggga atgatcagtg ccggcggcag ggtcgtccgg atcttcgccg cgatcggctg
     2341 gggatggggc ggcgactggg ccggcgccaa ggattaccag cacttctccg cctccggcga
     2401 ctagggctaa aatcccttta caccgtcgca ctgagttcgt aagcaccgct tcgataaagg
     2461 agtgcgaccc gactgaaagc gggtgcgatg gcaatggcgg agatcacaac cgaagtgcct
     2521 cacgggaccg cccggaggtt cgatttgcgg accggggatc tgatccggat cgagaccctg
     2581 gacggcgcgc agggaggcga cttcagcttc cccggtttcg atcaggccct gacccggaac
     2641 aagctcggct ggaagcggtt tggccggccc tggctggtct acgacgccca ggccggtgat
     2701 tcgctcttcg acgtcgacgg cgaggcgacc ttcgaactga tcgccctcga aggggaggga
     2761 gtcgccgaca tcatgtatcc gggttgctgg gacgaggtct acgaggaccg gcgaccgggt
     2821 tgccaggacc tgatctcggc ggcgctcacg attccccggc gggacctggc cggcatgctc
     2881 tccttcttca tcggctactc ggccgacaac tccgcttatc ggggtctggc cggcatcgac
     2941 ctgaagcccg gcgaccacct gaccttccgg gcgctgcgcg acgtcagctg cgcggtttcg
     3001 gcctgccccg accgggacat ccccggctgg agggtcggcg ccctgttcgt atcggtcacc
     3061 gaggccggca agtgaacggg gcgggcagct tccgcctggc ggtcgcccag ccggcgatcg
     3121 accgcgaagc tcccgaatcc tcccgggtgg cagaggctgg ggcgatgatc cgggaagccg
     3181 gtcgtggcga ggctgacctg atcctcttcc cggagagctt ccccggaccg atcaccgccg
     3241 gcctcgagta cgacgcggcc cccgatctcg gggctgcctg ccgtgactcc ggcgtcgcgg
     3301 cctgctgggg ccgggtcgag ccgatcggcg agggccgttt tgtcaccgtg gtgcggctca
     3361 ccgaccggac cggaggcgag gccggccgct acgttcgcag ccatcccgca accggtgacg
     3421 tccatccggt cctttccggg gtgccgatgg cgccgggccc ggagccgggc atcttcgagc
     3481 tggacggact caggctcggg atcgtggtct gttcagaact ctggaatccg gagctctgcc
     3541 gcgtcctggc cctgcgcggg gcggaggtac tgctcgcccc ggccggcggc ggcttccatt
     3601 ccgtggccga gaactggcag ctgatggcga gggcccgggc gatcgagaac gagatgttcg
     3661 tcgccctcac ccagcagctc tttgacggcg agtccggttc ggccctgatc gccgggccgg
     3721 agggtgtgct gagcgccgga acgggaccgg gcctcgtgat tgcggacctc gatctcgagc
     3781 gggcccgctg gctgcgaagc caggacgact cgatgcagga accgaagccg ttcagctccc
     3841 tgccggggct gctgagagcc cgacgccccg agctctatgg tgagctcggg gcgccacagg
     3901 aggggctcta cgacttcgag gccgccagca gctgaggctc ggggcgaagc gacgggcggg
     3961 gctcgcccgt cctggccgcg tcgcgatcgg tcttcgccgc gtcatcaccg accgcgtcct
     4021 tgccggggat cagcagggcc agcacggcac cgaccgccag cacggccacg gcgaccggca
     4081 ttgccgccac gaccccgtcg gtgaaggcct gggggctggt gaagccgccg ttggctgcga
     4141 agatcgacga gagcacggca actcccatga ccccgccgat ttcgcgaatc gcgttggtcg
     4201 ctcccgaggc cttgccggtt tcctcttccc ggaccgaggc gaccaccgtc gcggcggcgg
     4261 tggggaagac cagcgccatg ccggtgccgg ccatcatgaa gggggtcacg aaatcaccga
     4321 aggcggtgac cgggctggag atggccgtga tccaggcgag accgccggcc tgaagcacga
     4381 ggccggtgaa gatcagcggc cgggtgccga tccggtcgct gaggaagccg gccagcggcg
     4441 ccacgaacat cggcatcagg gtccagggga gggtcctgat cccggcgtcg aggggcccga
     4501 ggccctgcgc gacctggaag aactggctga gcaggaagat cgccccgaag gttccgaaga
     4561 acatcgcgaa cgaaagtccg ttggtggcgc tgaacccgcg cgaacggaag aaccgcatcg
     4621 gcagcatcgg ctcccgggcc ctcagctccc agacgatgaa ggcaccgatc aggagggccc
     4681 cggtgaccag cgaggcgagc accgtggtgc tggtccagcc gagcgactcg ccgcggatga
     4741 tcccgaaggt caggccgaag agcccggtgc cgaccagacc cagcccgggc aggtcgaggg
     4801 ccttgtcggg gccggtcgac tcgggcagcc gacgcagggc cagggggatc agggcgaggc
     4861 cgaccggcac gttcagccag aagatccagt gccaggagat gccttcgacg acggcaccgc
     4921 cgaagagcgg tccgagcgcg acgcccagtc cgctgatgcc gccccagatc ccgagtgccg
     4981 ccccccgctt ttcggccggg aaggctcccg agaccagggt cagggtgagc ggcacgacga
     5041 tcgccgcccc ggcaccctgc agtgcccgtg ccgcgaccag ggcatcgagc gtgggggcca
     5101 gcgccgaggc ggcactggcc gcggtgaaga gtcccacccc gatcgcgaac atgcggcggc
     5161 ggccgaagcg gtcaccgagt gcggcgccgg tgatcagcag aactgcgaag gccagggtgt
     5221 aggcgttcac ggtccattcg agcgattcga tcgtgccacc gaggtcagcc cggatcgagg
     5281 gcaacgcgac cccgacgacc aggttgtcga gggaggtcat gaagagcgcc agcccggtga
     5341 tgatgagcgt ccagatggcg ctcctgtttc cggagctctc gcccggtctg cttgtgatgc
     5401 tggccgtcat gacggcctcc ttcccggagc cgaagccccg tttgattcgt ttggtcggtc
     5461 gggcccctgc gagcccgttt cggttacttc ctgtgtggtg tgtgactggt tactcacttt
     5521 gatttcctga taatgtgtga ttgctcactc actaatcggg tctgaaaaag cggcctcctg
     5581 gccgcgtcgg ttcagtcctc ccgctcgtgc ggtcggctgc cggtcagctc tcgcgcggga
     5641 agcagtcccc ttcgaagtcg gtcagggcca cggcccaggg ttcgtccacc tcttcggcat
     5701 tgatcgcggt catcacgttg atcagcatgc cgtgggcgaa ccagttcttc atctcttcgc
     5761 cgttgccgcc ggtgcgctcg gagccgagcg cgtagaggtt ggcgaaacac ttgcgcatcg
     5821 cctcccgcac ggccgggatg gcgggcgaaa cggcctgggc gtgaagctgc atcaggagca
     5881 actcgcggtc gctgctgacc aggtcaccgt aggcgaggcc catggcatcg ccgggttcga
     5941 ttccgttctt ctcggcctcc gaagccacgg cggcgagagt ctcgaccatg cgcccggtcg
     6001 accgttcgac cgcggccacg aagagttcct ccttggtcgg gaagagccgg aaaaggtagg
     6061 cctgcgaaat gccggccttc ttcgcgatct cggtggtcgg ggcggcgtgg taacccttct
     6121 cggcaaagac cggaatcgcc gccgtaatca gttcctcacg gcgctcttca gcggtcgaaa
     6181 gcgagcggga tcccgtcgag cgggtcctgg ccgtcccggt ccccggggac gctgcgatct
     6241 tgtccgaagt cgttgccatc aggtgtgagt atgcactcac tcacaccatt tgtcaagtcg
     6301 acggcctgaa ttcactgcta aagccacctg acatccgacc tcagaaagga aagatgccgc
     6361 cgcctttctg gcggccgttg ctggcgtacc agggattgat cttcccgtcg ggtcccttgc
     6421 ggcgcctgag aatcagaaag aggatcaggt agacgccggc gacggtgccg aacaccttga
     6481 ccacgagcgg cccgattacc agtccgacga tcaccgcccc cgcaatcaag ccgatcaaca
     6541 tcaggtgcca cttcctccgg accagatctc ccatatcgcg atcttaatcc cggatcccgg
     6601 caaccggggc caagtggccg aggtgggtat gaaattcgtt catatagaca cttttcatac
     6661 ccacctcaaa atgggatccg ggcgactgac ctggcgagga cggaaagcca cgcgtattgc
     6721 atatgaatcc gggtctgccc aggaaaaagg ccccggatct ctccggggcc ctttcgaaac
     6781 gagttgaact caacctgggt ggttcatcca catccggtgt tgttgccgca ggacgagcag
     6841 gtgtagcagg cgccggttcg ctgcatcatg cctccgcact ggtcacaggc cgggcccagc
     6901 tctgctgccc cgggagtgaa gtggagcctg gccgggacca ccgggacgtc atcggtgagc
     6961 gccgtgccgg ccgggaccgc ggagccgccg gtatcggcga ccggggccgc cgactcggtt
     7021 gatgcggacg gggccgagcc accgttgccg ttgccgccgt tgccattccc gttgccaccg
     7081 ttgccggccg ggctgccgtt gccggagccg ttggccaggt tgatgccggc attctcggct
     7141 tccttgcggt tgcggacctc ctgggtcatg atcccctgct cctcgaggaa gtcgggatcg
     7201 tcgatgaact gggaggcgag ccagcgcatg atgtagtccg gcatcgactt ggcgaagggg
     7261 atgtccgggt tgccggtgat cccttccggg tcgaaccgca tgtagctgaa cttgctcacg
     7321 aatacctcga gcggcacccc gtactggagg ccgagcgaga tcgcggtcgc gaaggcattc
     7381 atcatgcccc gcatggtcga gccttccttg ccgatgtcgg tcaggaagat ctcgccgacc
     7441 gagccgtcgg tgtacttgcc ggccgtgatg taaccctcgt ggccgccgac cgagaacttg
     7501 tgggtgaccg agtcgcgggt ctttggcatc cggtgacgga ccggctcgtt cggggccgcc
     7561 tcggcttccg cttccttctc cttgtccttc tgggcgtcgg tgcggagcgc ctgggcggtc
     7621 ttggaaccgt cacggtagat cgcgagggct ttgaggccgc cttcccagcc gcgagtgtag
     7681 gcgtcggcga tgtcctcgac ggtcgccgag ttcggcatgt tgaccgtctt cgagatcgcg
     7741 ccggagagga acggctgggt cgccgccatc atgtcgatgt ggccggtgtg gctgatcgcc
     7801 cgctcgccga ccgcgacgtc gaagaccggc agatgctctt ccttcagggc cggggcgccg
     7861 atgatcgtgc cgtgctcgtt gatgtaggcc tcgatctgcg agatctcgga ttccgagtac
     7921 tcgagcgtgc gaagggccag cggcacggac ttgttgacga tggtcatgtt gccgccgccg
     7981 accagttcct tgaacttgac cagcgagaag tccggctcga tgccggtcgt gtcgcagtcc
     8041 atcaggaagc tgatcgtgcc ggtcggggcc agcaccgtcg cctgggcgtt gcggtagccg
     8101 tgctcatcgc cggtcgcgac cgcgacgtcc cactcctcgc gggccgcgtt cagcagctcc
     8161 tcgtggacgc cttcgccgct gatctcgtag gccgcatcgc ggtgcatgcg catcacgttg
     8221 ttgtgcggct cgcggttcag ctcgtaggcg tcgtaggggc ccatcgccgc ggcaacctcg
     8281 gccgaacggc ggtaggcgcg accggtcatc agcgaggtga tcgcggcggc gacgttgcga
     8341 ccctcatccg agtcgtaggg catgccgtcc gacatcagca gggcgccgag gttggcgtaa
     8401 ccgaggccga gctggcggaa ggcccgggcg ttcttgccga tttcctcggt cgggtagctc
     8461 gaggggctga cgatgatctc ctgggcgagg aagacggtgt cgaccacggc gcagaaaccg
     8521 tcgacgtcaa agctgccgtc ctcgcgacgg aacttcatca ggttgatcga agcgagattg
     8581 caggccgagt catcgacatg catgtactcc gagcaggggt tcgaggcgtt gatccggccc
     8641 gagttcgggc aggtgtgcca cttgttgatg atcgtgtcgt actggacgcc cggatcggcg
     8701 cactgccagg cggcgtcggc gatctggttc atcaggtcgc gggccgacat ggtcttggtg
     8761 accgagccgt cggtgcgggc gagcaggttc cactcggcgt cgtcggcgac cgcctgcatg
     8821 aactcgtcgg a
//
LOCUS       DAOQKN010000094         8802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_3826_length_8802_cov_5.622156, whole genome shotgun sequence.
ACCESSION   DAOQKN010000094 DAOQKN010000000
VERSION     DAOQKN010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 8802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8802
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3826_length_8802_cov_5.622156"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1324)
                     /locus_tag="PLJ64_07485"
     CDS             complement(<1..1324)
                     /locus_tag="PLJ64_07485"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNC93359.1"
                     /translation="MPLAGLVDEGQESPWLESGSEPFAPVSEDIRCDVAVVGGGIVGV
                     STAYRLARAGCSVVLVEARRLAAGVTGHSSAKLSALQGLAYSAIADRNGGEAASAYAE
                     LNARGIRFVEETTAALGIDCGLTHRPAVSYAETDEGRENLALEMDAALAAGLAVTDED
                     LAPLPFPVAATLTLENQAHFDPVKWVRGLAGALTELGGRVHETSRVTGVDGLRGNRKV
                     KMENGVTIEAERVVLATHVPILDRAAFYARVKPLTSFAVAGQIEGPLPQGLYLSVDGP
                     TRSISPLPGGGGTRSLLVGGESHRPGTEDSAVSVARLRQFMRSRFEVRSVDCAWGAHD
                     MMSFDRLPIVGRLLPFDSRILTATGFSKWGLAAGAGSSELLADQLLGEDNPVAATFDP
                     VRLRLSSVPTLVRHNLESGAMLLADRINRRRPRRRLQPGEGMVVGDGVG"
     gene            complement(1366..1998)
                     /locus_tag="PLJ64_07490"
     CDS             complement(1366..1998)
                     /locus_tag="PLJ64_07490"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNC93360.1"
                     /translation="MAEALIIDIDGTLVDSNYQHVIAWQEALREHGLDAEAWKIHRYV
                     GKGGDKMIESVAGEEAERKAGDAVRESEGEIFQRLIDQVRPLEGAGEFVRKVKDLGFI
                     VVLSSSAKDHEVDHYLDQLEVRELIDGHTSSADVDQTKPDPDLIEAALEKAGTRDALM
                     VGDSIWDVEAARRSGIDCLAVLTGGFSKAELAAAGAIEVRPSLADLEIPD"
     gene            complement(2003..2482)
                     /locus_tag="PLJ64_07495"
     CDS             complement(2003..2482)
                     /locus_tag="PLJ64_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93361.1"
                     /translation="MAEISTVEEKLAEVTGLAQAAQGAIEKVAPMIDDESVSESLEKM
                     KQEAVETEERCTSAIEKRDGRKTAILDKARETKQEATEMMSTYLGDDSDGLDGLEFMT
                     MAEAGEVGHWEILGELSQRAGESELEELTGWALPIQERHLAEVRKGSLTLASQEDID"
     gene            complement(2488..2931)
                     /locus_tag="PLJ64_07500"
     CDS             complement(2488..2931)
                     /locus_tag="PLJ64_07500"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HNC93362.1"
                     /translation="MIVFLVVDMISRWDFDGADRAADRAGDAVEAINRLRRRAGENLK
                     VVYANDMDGGFHGSRETAFALAMSGRHPELVSPLEPRPDEDFIHKGQHSAFYGTPLAH
                     LLDVCDVETIWLAGQATEQCILYTALDAHVRGSGYYRSQSTPYWR"
     gene            complement(2958..3224)
                     /locus_tag="PLJ64_07505"
     CDS             complement(2958..3224)
                     /locus_tag="PLJ64_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HNC93363.1"
                     /translation="MPKGSDHGPSVKDDKLYEELREDGASKEKAARIANAKAGGENVS
                     KKGGESEDYEDRTVEELQDRAAEIGIEGRSDMNKKELISALRNH"
     gene            3344..3814
                     /locus_tag="PLJ64_07510"
     CDS             3344..3814
                     /locus_tag="PLJ64_07510"
                     /inference="COORDINATES: protein motif:HMM:NF039409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6328 family protein"
                     /protein_id="HNC93364.1"
                     /translation="MGSSPRDEENWQRLDRNLDELLQELRIALPGVQVLFAFLLAVPF
                     QQRFGDVTDFQQSVYFATLMLTGASTFLLIAPTAFHRVTFRQQQKERLVRLANRLAIA
                     GLGCLGLAVIGAVTLVTDFLYGAAPTALAAGAFLVIATVLWVVIPVRVRTRHRD"
     gene            complement(3829..4389)
                     /locus_tag="PLJ64_07515"
     CDS             complement(3829..4389)
                     /locus_tag="PLJ64_07515"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HNC93365.1"
                     /translation="MSQEQMNQKLKDKRVAILVANEGVEEIELKGPLQALRDAGAEVD
                     LVAPEEGQVQAFNHLDKGSPFTVDRTVSEVSSDEYAALVLPGGVANPDNLRTHEDAVA
                     FAGSFFEAGKPVGAICHAPWTLVEAGVLEGRTLTSWPSLKTDIENAGATWVDEEVHVD
                     GEFVTSRRPDDLESFCHQVVETIAAA"
     gene            4521..5114
                     /locus_tag="PLJ64_07520"
     CDS             4521..5114
                     /locus_tag="PLJ64_07520"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNC93366.1"
                     /translation="MKALTINCTLKASPATSNTEALATVVADEIQQAGVETDLVRAVD
                     HNILPGVSNDEGEGDGWPAVLDRVRAAQILIIATPTWLGQPSSVAKRVLERMDALISE
                     TDDEGVPFAYGRVAGVVVTGNEDGAHHVISEISGSLIDIGYTIPGQAWTYWNRGPGPG
                     DDYLSNEAGHDWSHETGKTAARNLLTAARSLEGAWSG"
     gene            complement(5119..6036)
                     /locus_tag="PLJ64_07525"
     CDS             complement(5119..6036)
                     /locus_tag="PLJ64_07525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HNC93367.1"
                     /translation="MPRPLWKGTISFGLVSVPVEMWSAIRDRNIRFREIHVEDGSPVR
                     HVRYCAEEGKPVDPSEVGRAYELDDGRLVRITDEELEGLEPEKTQTIEIESFAPRAQV
                     EPLRFDRPYNLIPGNQTVGTIRAYRLLVEAMAESDLLALGKFVMRGREYLAAIRVREN
                     VLTLSTMHFPAEIRSTEDVPGPAKKADKEAVKDAVAVIEERSVEWDPGKYPNEHRERL
                     LAVIRKKDKGRKIKAPAPVAEERPTGAPDLMAALKKTLAESRSKRRKPRDLARLSKEE
                     LYEMASERGLKGRSSMNKQQLLKALRANR"
     gene            complement(6036..6815)
                     /locus_tag="PLJ64_07530"
     CDS             complement(6036..6815)
                     /locus_tag="PLJ64_07530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HNC93368.1"
                     /translation="MALRSIWNGTISFGLVKVPIKLYSATEPKGIAFREIHVKDNSLL
                     QHRRVCKAEGREVDRSEIAKGYEVRPDQYVVLESDEVKAAAGERPKTIEIEEFVDVEA
                     IDPFYFNKSYFMGVRDVDEPYALLVAALRDSGKAGIGRFTFHNREYLVALHAEEDRLV
                     LHTLRYAEEIIEPGDLELPDGGKKPTRKEVELARRLVEGLTEDFDPGEYQDEYRASVM
                     DLIERKAEGKKPKKKRARKREETGDLSKALEKSLAAQGSRS"
     gene            6908..7816
                     /gene="ligD"
                     /locus_tag="PLJ64_07535"
     CDS             6908..7816
                     /gene="ligD"
                     /locus_tag="PLJ64_07535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HNC93369.1"
                     /translation="MKARLGDPGKGVAWNFEVKWDGYRAIAFCSGGLKLQGRRLNEIG
                     ADFPEIKPLGSDSRARGLVLDGELVVVDEDGRPDFQLMQSRRERQLEAHFMIFDLLWA
                     DGTDLRPMPYLERRARLEALGLTGPTWSVPERLAGELNEVLEATAALGLEGVVAKDPD
                     SPYVSGRRTNYWLKVKHVKRQEFVIGGWLPGKGHRSGSLGSLLVGYYDGNSELRLAGR
                     VGTGMGDELLAELFATLSASARVDSPFVAEDAASIPRGAGWCEPELVIEVRFTEWTRE
                     GRLRNPVFVGFRPDKSPREVVREEPA"
     gene            7813..8769
                     /gene="ligD"
                     /locus_tag="PLJ64_07540"
     CDS             7813..8769
                     /gene="ligD"
                     /locus_tag="PLJ64_07540"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HNC93370.1"
                     /translation="MTFGWKRSERLTSAVTGGPGKASIEIDGRELRLTNLDRVLYPET
                     GFTKGDLIDYYAAVAPLLLPHLRDRPVTMRRFPDGVESEGFWEKHCPDHRPGWVKTAS
                     IESSSSSKGRVDYCLVNDVATLVWMANLGCIELHVSLARARRRKTPDFMVFDLDPGPP
                     ADILDCAETALLIHDTLAGQDLVSLAKVSGSKGIQVYVPLNGRASYDRTGGFAHSLAR
                     AFEQELPDQIVSRMTKSLRKGKILIDWSQNSEHKTTVSVYSMRARSTPSVSMPVGWER
                     LEQALDRSDDSLLRFGPAEVLRRISGTDPFGAILELKQKLPG"
BASE COUNT         1383 a         2964 c         2749 g         1706 t
ORIGIN      
        1 ggccgacgcc gtcgccaacc accatgccct cgcccggctg caggcggcgc cggggccggc
       61 ggcggttgat ccggtcggcc agcagcatgg ctccggattc gagattgtgc cggaccaggg
      121 tcggcaccga actcagccgt aaacggacgg ggtcgaaggt tgccgcgacc gggttgtctt
      181 cccccagcaa ttgatctgcg agcagctcgg aactgcctgc gcccgcggcg agaccccatt
      241 tggagaatcc ggtcgcggtg aggattctcg agtcgaaggg cagcagccgc ccgacgatcg
      301 gcaggcggtc gaagctcatc atgtcgtggg cgccccaggc acagtccacg gagcggacct
      361 cgaaccttga cctcatgaac tggcgcaggc gggcaacgct cacggcactg tcctcggtgc
      421 ccggacgatg gctctctccg ccgaccagca acgagcgcgt acctcccccg ccgggcaggg
      481 ggctgatcga gcgggtcggg ccgtcgacac tcaggtagag accctgcggg agcggcccct
      541 cgatctggcc ggcgaccgcg aaggaggtca ggggcttgac ccgggcgtag aaggcggcgc
      601 ggtcgagaat cggtacgtgt gtggcaagca ccacccgttc ggcctcgatc gtcacgccgt
      661 tttccatttt gaccttgcgg ttgccccgca atccgtcgac cccggtcacc cggcttgttt
      721 catgaacccg gccgccgagc tcggtcaggg cgccggcgag acccctgacc catttgaccg
      781 ggtcgaagtg ggcctggttt tcgagggtga gcgtcgcggc aaccggaaac ggcaagggcg
      841 ccagatcctc gtcggtcacc gcgaggccgg cggccagcgc cgcgtccatc tccagggcca
      901 ggttctcccg tccttcatcg gtttcggcgt acgaaaccgc gggccggtgg gtgagcccgc
      961 agtcgattcc cagtgccgcg gtcgtctcct cgacgaagcg gatgccccgg gcgttcagtt
     1021 cggcataggc ggaggccgct tcgcccccgt tcctgtcagc gattgccgag taggccagac
     1081 cctgaagcgc gctcagcttg gccgacgagt gaccggtcac accggcggcg agccgtcgcg
     1141 cctcgaccag gaccaccgag catcccgccc gggcgagccg gtaagccgtc gagaccccga
     1201 cgattccgcc acccaccacc gccacgtcgc agcggatgtc ttcgctgacc ggggcaaacg
     1261 gctccgaccc gctttccagc cagggcgatt cctggccctc gtccaccagg cccgcgagcg
     1321 gcaccggctc tttacttcct gtgccttcgg gcgccatggc gttggtcagt ccgggatttc
     1381 cagatcggcc agggacggcc ttacctcgat tgccccggcc gccgcgagtt ccgccttcga
     1441 gaagccgccc gtcaagacgg cgaggcagtc gatccccgag cgccgggccg cctccacgtc
     1501 ccagatcgaa tcgccaacca tcagggcgtc acgggtcccc gccttctcca gggccgcttc
     1561 gatcaggtcc ggatcgggct tggtctggtc cacgtcggcg gaactggtgt gcccgtcgat
     1621 caattccctg acctcgagct ggtcgaggta atggtcgacc tcgtggtcct tggccgaact
     1681 cgaaagcacg acgatgaagc cgaggtcctt cacctttcgc acgaattcgc cggcgccttc
     1741 cagcggcctg acctgatcga tcagccgctg gaagatctct ccttcacttt cgcggaccgc
     1801 gtcgccggct ttccgctcgg cttcctcgcc ggcaacggat tcgatcatct tgtcgccacc
     1861 cttgcccacg tagcgatgga tcttccacgc ttcggcgtcg agcccgtgct ctctcagggc
     1921 ttcctgccag gcgatcacgt gctggtagtt ggagtcgacc agggtgccgt cgatgtcgat
     1981 gatcagagct tctgccatga agtcagtcga tgtcttcctg gctggcaagg gtcagcgagc
     2041 ccttccttac ttcggccagg tgacgctcct ggatcggcag cgcccatccc gtgagttcct
     2101 caagttcgga ttctccggcc cgctgactga gttcacccag gatctcccag tggcccacct
     2161 cgccggcctc ggccatggtc atgaactcga gtccgtcgag cccgtccgag tcgtcaccga
     2221 ggtaggtcga catcatctcg gtcgcttcct gcttggtttc gcgggccttg tcgagaatcg
     2281 cggtcttgcg accgtcccgc ttctcgatcg cgctggtgca ccgctcctcg gtctcgaccg
     2341 cttcctgctt catcttctcc aggctctccg acaccgattc gtcgtcgatc atcggtgcga
     2401 ccttctcgat cgctccctgg gctgcctggg ccaggccagt gacctcggcg agcttctcct
     2461 cgaccgtcga tatttctgcc atttcgatta cctccagtaa ggggttgact ggctgcggta
     2521 gtacccgctg cccctgacgt gggcgtccag tgccgtgtag aggatgcact gctcggtcgc
     2581 ctggccggcc agccagatcg tttccacgtc gcagacgtcg agcaggtgtg ccagcggcgt
     2641 gccgtagaag gccgagtgct gacccttgtg gatgaagtcc tcatcgggac ggggttcgag
     2701 gggtgaaacc agttccgggt gccggcccga catcgccagg gcaaaagctg tttcccggga
     2761 tccgtggaag cccccgtcca tgtcgtttgc gtaaacgacc ttcaggttct ccccggcacg
     2821 gcggcgcagc cggttgatcg cttcgactgc gtcaccggcc cgatccgccg cccggtccgc
     2881 accgtcgaaa tcccagcgag agatcatgtc gacgacgagg aatacgatca tcgcgctggc
     2941 cgtccggccg tcaggggtca gtgattgcgc agggcgctga tcagttcctt cttgttcatg
     3001 tcggagcggc cttctatgcc gatctcggcg gcccggtcct gcagttcctc gacggtgcga
     3061 tcctcgtaat cctcggattc gccgcctttc ttgcttacgt tctctccgcc cgccttcgcg
     3121 ttggcgatcc gggccgcttt ttccttgctg gcgccgtctt cgcggagttc ttcgtagagc
     3181 ttgtcgtcct tgacgctcgg tccgtggtct gatcctttcg gcatttcgag tctcctttgc
     3241 gttcgaattt gccagtcaac cttggctacc gtttctggta cccgacggtt gcgcgtcaaa
     3301 accagtccgg ttcgagtcga ggccgtccgg gtacctcggg ggtatgggat cgagcccgcg
     3361 cgatgaggag aattggcaac gccttgaccg gaacctggac gagttgctcc aggaactcag
     3421 gatcgccctg cccggagtcc aggtcctctt cgcgtttctg ctggcggttc cgttccagca
     3481 gcgtttcggc gatgtgaccg attttcagca gtcggtctat ttcgcgaccc tgatgctgac
     3541 gggggcctcg acctttctgt tgatcgcccc gaccgcgttt caccgcgtga catttcgcca
     3601 gcagcagaag gaacgcctcg tcagacttgc caatcgcctg gccattgcag ggctcggctg
     3661 cctcggtctg gccgtgatcg gcgcggtgac gctggttacc gactttctct acggcgcggc
     3721 ccccacggcc ctcgcggcag gagccttcct ggtgatcgcg accgtgttgt gggtggtcat
     3781 cccggtccgg gttcgcaccc ggcaccggga ctgacccgcg gacggagctc aggcggcggc
     3841 gatcgtctct acgacctggt gacagaacga ttcgaggtcg tccgggcgcc ggctggtgac
     3901 gaactcgccg tcgacatgga cctcctcgtc gacccaggtg gcgccggcgt tttcgatgtc
     3961 cgtcttcagg cttggccacg aggtcagcgt tcggccttcc aggacgccgg cctcgaccag
     4021 ggtccagggg gcatggcaga tcgctccgac cggcttgccc gcctcgaaga aggaaccggc
     4081 aaaggcgacc gcgtcttcgt gggttctcag gttgtccggg tttgccacgc cacccgggag
     4141 cacgagggct gcgtactcgt cactcgaaac ctcggaaacg gtccggtcca cggtgaaggg
     4201 acttcccttg tcgaggtgat tgaaggcctg gacctgaccc tcctcgggtg cgaccaggtc
     4261 aacttccgct ccggcgtccc tcagcgcctg cagcggcccc ttcagttcga tttcctcgac
     4321 gccctcgttg gccacgagga tcgccacacg cttgtccttc agcttctgat tcatctgttc
     4381 ctggctcatc gtttctcctc ttgtgggttc aattaccggt tacccgttcg gcccccggtc
     4441 aaccctggat ccgcgtccgg acccgccctg attctggtgg cggcacgttt tggccttccc
     4501 ggcaggggta ccggccggga atgaaagcgt tgaccatcaa ctgcaccctg aaggccagtc
     4561 cggccacctc aaacaccgaa gccctggcaa ccgtggtcgc cgacgaaatc cagcaggccg
     4621 gggtcgagac cgacctggtc cgggcggtgg accacaacat ccttcccgga gtcagcaacg
     4681 atgaaggcga aggtgacggc tggcctgcag tgctcgaccg ggtccgcgcg gcgcagatcc
     4741 tgatcatcgc gacgccgacc tggctcggtc agccttcgag cgtcgccaag cgggtgctgg
     4801 aacgaatgga cgcgctgatc agcgagaccg acgatgaagg cgttccgttt gcctacggcc
     4861 gggtcgccgg cgtggtggtg accgggaatg aagacggcgc gcatcatgtg atcagcgaga
     4921 tctccggcag cctgatcgac atcggctaca cgattcccgg tcaggcatgg acctactgga
     4981 accggggtcc cggaccgggt gatgactacc tttcgaatga agccgggcac gactggtccc
     5041 acgagaccgg gaagactgca gccaggaacc tgctcaccgc cgcccgttcc ctcgaaggag
     5101 cctggtcggg ctgaccttct agcggttggc gcggagcgcc ttgagcagct gctgcttgtt
     5161 catcgaggag cggcccttga ggcctcgttc ggaggccatc tcgtaaagct cctcctttga
     5221 gagccgggcg aggtcgcgcg gcttcctgcg cttgctgcgg gattccgcca gggtcttctt
     5281 cagggcggcc atcagatccg gggcaccggt cggccgctct tcggcgacgg gagcgggagc
     5341 cttgatcttc cggcccttgt ccttcttcct gatcactgcc agcagccgct cacgatgttc
     5401 gttcggatac ttgccgggat cccattcgac ggagcgttcc tcgatcaccg cgacggcgtc
     5461 cttgaccgcc tccttgtccg ctttcttcgc cggtcccggc acgtcctccg tagatcgaat
     5521 ctcggccggg aaatgcatcg tcgagagcgt gagaacattt tcccggaccc ggatcgcggc
     5581 caggtactcc cgtccccgca tcacgaactt gcccaacgca agcaggtcgc tttctgccat
     5641 cgcctcgacc aggagacggt acgcacggat cgtgcccacg gtctggttgc cgggaatcag
     5701 gttgtacggc cggtcaaagc ggagcggttc gacctgggcg cgcggggcga aggattcgat
     5761 ctcgatggtc tgtgtcttct ccggttcgag tccctccagt tcctcgtcgg tgatccggac
     5821 caggcggccg tcatcgagtt cgtaggcacg accgacttcc gagggatcga ccggtttgcc
     5881 ttcttcggcg cagtagcgca cgtgcctgac cggcgaaccg tcttccacgt ggatttcacg
     5941 gaacctgatg ttgcggtccc ggatcgccga ccacatctcg accggcacgc tgaccaggcc
     6001 gaacgaaatc gtccccttcc agagtggtcg cggcatcagg aacgactccc ctgcgcggcg
     6061 aggctctttt cgagcgcctt ggaaaggtcc ccggtctcct cacgcttgcg ggcccgcttc
     6121 ttcttcggct tcttgccctc ggccttccgc tcgatcagat ccatcacgga cgcgcggtac
     6181 tcgtcctggt actcgccggg gtcgaagtcc tcggtcagtc cctcgaccag ccggcgcgcc
     6241 agctcgactt ccttgcgggt cggcttcttg ccgccgtccg gcagttcgag gtcacccggc
     6301 tcgatgatct cctcggcgta cctcagtgtg tggagcacca gcctgtcctc ctcggcgtga
     6361 agggcgacca ggtactcgcg gttgtggaag gtaaaccggc cgatccctgc cttgccgctg
     6421 tcccggagtg ccgcgacaag cagcgcgtaa ggctcgtcca catcccgcac acccatgaag
     6481 taggacttgt tgaagtagaa gggatcgatc gcctcgacat cgacgaactc ctcgatctcg
     6541 atcgtcttcg gccgctcgcc ggccgccgcc tttacctcat cactctccag cacgacgtac
     6601 tggtcggggc gcacctcata gcccttggcg atctcgcttc ggtcaacttc cctgccctcc
     6661 gccttgcaga cccggcggtg ctgcagcagc gaattgtcct tcacgtgaat ctcgcgaaag
     6721 gcgattccct tcggttcggt cgcagagtac agtttgatcg ggaccttgac cagcccgaag
     6781 gagatggttc cgttccagat tgaccgcaac gccatgcggt gagcgtaccc gcgaaccgct
     6841 tcatccacac catcgcgggt attcggtaga catgatgcaa gcgatgttgc ccggtccaat
     6901 cgaacccatg aaagcccggc tcggcgatcc cggcaagggg gtcgcctgga actttgaagt
     6961 caagtgggac ggttaccggg ccatcgcctt ctgttccgga ggcctgaaac tccagggccg
     7021 gaggctcaac gagatcggcg ccgactttcc cgaaatcaag ccgctcggtt ccgactcccg
     7081 cgcccgcggc ctggtcctcg acggcgagct ggtggtagtt gacgaggatg gccggccgga
     7141 cttccagttg atgcagtccc gacgcgagcg tcaactcgaa gcgcatttca tgatcttcga
     7201 cctgctctgg gccgacggca ccgacctcag gccgatgccc tacctcgaac gccgcgcgcg
     7261 gctggaggcc ctcggtctca cgggcccgac ctggtcggtc ccggaacggc tcgccggaga
     7321 actgaacgag gtgctcgaag ccacggccgc actcgggctc gaaggggttg tcgccaagga
     7381 tcccgacagc ccctatgtgt cgggccgacg gacgaactac tggctcaagg tcaagcacgt
     7441 caaacggcag gaattcgtga ttggcggctg gctgcccggc aagggtcacc gttccggttc
     7501 gctgggttcg ctcctggtcg gctactacga cggcaactcc gaactccggc tggccggcag
     7561 ggtcggcacc ggaatgggtg acgaactgct ggccgaactc ttcgcgaccc tttctgcttc
     7621 ggcccgggtc gactccccgt tcgtcgcgga agacgctgcc tcgatacccc ggggcgccgg
     7681 ctggtgtgaa ccggaactgg tgatcgaggt caggttcacc gagtggaccc gcgaaggacg
     7741 actgcggaat ccggtcttcg tcgggttcag gccggacaag tcgcctcggg aagttgtccg
     7801 ggaggagccg gcatgacctt cggctggaag cgcagcgaac gactcacctc ggcggtcacc
     7861 gggggcccgg gcaaggcatc gatcgagatc gacggcaggg aactcaggct gacgaatctc
     7921 gaccgggtgc tctacccgga gaccggtttc acaaagggcg acctgatcga ctactacgcc
     7981 gccgtggccc ccttgctcct cccccatctc cgcgaccgtc ccgtcaccat gcgtaggttc
     8041 cccgacgggg tcgaaagcga aggcttctgg gagaagcatt gtcccgacca tcgccccggc
     8101 tgggtcaaga ctgcctcgat cgagagcagc tcatcctcga aggggcgggt tgactactgc
     8161 ctggtcaacg acgtcgccac cctcgtctgg atggcgaacc tgggatgcat cgaactccat
     8221 gtttcactgg cccgggccag gcgccggaag acgccggact tcatggtctt cgacctcgac
     8281 ccgggcccgc cggctgacat cctcgattgt gccgagactg ccttgctgat ccacgacacg
     8341 ctcgccggcc aggatctcgt ctcgctggcg aaggtctcgg gctcaaaggg aatccaggtt
     8401 tacgttccgc tcaacgggcg ggccagctac gaccggaccg gaggtttcgc tcattcactg
     8461 gcccgggcct tcgaacagga attgccggac cagatcgttt cgcggatgac gaaaagcctg
     8521 cgcaagggaa agatcctgat cgactggagc cagaactccg aacacaagac caccgtctcg
     8581 gtgtactcca tgcgggccag gagcaccccg tcggtttcga tgcccgtcgg ctgggagcga
     8641 ctcgagcagg cgctcgaccg ctcggacgac tcgctgctcc gcttcgggcc cgcggaagtg
     8701 cttcgcagga taagcggcac cgatccgttc ggcgccatcc tcgagctgaa gcagaagctg
     8761 cccggctgac ccgggagagg gagtcaagga ttggggcgcc gc
//
LOCUS       DAOQKN010000095         8791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_3830_length_8791_cov_5.559638, whole genome shotgun sequence.
ACCESSION   DAOQKN010000095 DAOQKN010000000
VERSION     DAOQKN010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 8791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8791
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3830_length_8791_cov_5.559638"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..1236
                     /locus_tag="PLJ64_07545"
     CDS             88..1236
                     /locus_tag="PLJ64_07545"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNC93371.1"
                     /translation="MAKRATKRGDERTDLTGSRDVLICGASFAGLMVARQLAGTGADV
                     LVVDRYEIGERQTSACAIPTAWLEELGLIESEKQRFDSVLIHTAHGSIRLPVPWSFST
                     FDYPSFCEMLWAQCDAEFETAKVNGRAKTPEGDHVIDTDRGEITAPLVVDSLGWKRVL
                     AAGAHYQPPNQPLSRGLEVHPPGGNVDLEVFINRRYVPAGYGWSFPADDEVRVGVGSF
                     DPHFPVRPTTDLIADDLNVEKSAYQGNWIPHKLRPATGDGIFFAGDSAGHCFPLTAEG
                     IRPALYFGAALGMELRDVVEGRKSREEALTCYHDFSAGHQWAFDWLLRAQKLLPKVHP
                     RLVKPTLAASFKAGGSGDYAFNKYLDIAPPEFAALATRALKENSPVPT"
     gene            complement(1233..2744)
                     /locus_tag="PLJ64_07550"
     CDS             complement(1233..2744)
                     /locus_tag="PLJ64_07550"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102662 - malonate-semialdehyde
                     dehydrogenase (acetylating, NAD+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HNC93372.1"
                     /translation="MTVSTETETTTLQNYVGGKWVEAGGAELHDVVNPANGEVIARVP
                     FSTASHLDEAVRAARKALPAWRGVSTIARARMLFTLREKLEARKDEIARCVVTEMGKT
                     LPDATAEVGRMIEMVEAATAIPTTMQGKVLEDVARGVDAETIRQPVGVCGIIAPFNFP
                     AMVPFWFLPFAIACGNTIVLKPSEQVPMTQQKVFGIIDELDLPDGVVNLVNGSREVVE
                     GMLDHPDIDAISFVGSAPVAQLVYERAAQTGKRVQALGGAKNHMVICPDAGVEPTVNG
                     LIGSGFGAAGQRCMAGSVIVTVGSGHDSVVPKLVEETKKLHVGNGLDENIDVGPVISC
                     DARDRIDGWVAKAIEEGAEVLVDGRTPLPDAPEGSSYVGPTILDNVKPTDSIIENEVF
                     GPVIAIVNVDTLEEAIEVVNASPFGNGVSIFTESASAVRKFRHDVEVGMVGVNIGVAA
                     PVAFFPFSGWKASFLGDLHAHGPDGVEFFTRKKTITSRYFSGGHESGSYFVER"
     gene            complement(2863..3291)
                     /locus_tag="PLJ64_07555"
     CDS             complement(2863..3291)
                     /locus_tag="PLJ64_07555"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HNC93373.1"
                     /translation="MVLLVLALGAGVAACGSDSDSGSGEGSEASAVEGVTWQAMNLVT
                     QGAATSLPKGVKPPTLEINDGQASIFAGCNSGSGEATVNEATIDFGPIALTRKSCEQV
                     QNQIEFLVTQVLQGEVKYEVNSDGNLILEKGTDSIVYTKG"
     gene            3357..4643
                     /locus_tag="PLJ64_07560"
     CDS             3357..4643
                     /locus_tag="PLJ64_07560"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC93374.1"
                     /translation="MTSTPEQVQRVYLTLMLGNTLAASFIWGINTIFLLDAGLSNLEA
                     FAANAFFTAGMVIFEIPTGVVADTIGRRVSYMAGTLTLMATTLLYVMLWEINGPFWAW
                     AVISLLLGLGFTFFSGAVDAWLVDALDETGFTGDLERVFGRGMTVTGIGMLAGSVIGG
                     YVAQVTSLGVPFVMRAVVLLAMFFFAAWAMKDLGFKPHKAESTFGEMKSITRNSIDYG
                     WRVPSVKWIMLAAPFTAGVGIYAFYALQPYLLELVGDPHAYGIAGITAALVAGSQIIG
                     GFLAPRIRRRFRLRTSALLLAVSVGAVTLALIGVIEQFWVAVGLIATWAMLSSAATPI
                     RQSYINGMIPSRQRATILSFDSMLGSTGGVVVQPALGRVADTSGYATSYLYGAMITVF
                     ALPFILLSRHQHPPADRATGTPEAEGTVEAGPESDR"
     gene            complement(4647..5048)
                     /locus_tag="PLJ64_07565"
     CDS             complement(4647..5048)
                     /locus_tag="PLJ64_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HNC93375.1"
                     /translation="MLFGQQHVDRYQATGGEEGHDWQGTHTLLLTTTGRKSGEKRTTP
                     LIYDPVGDAYAIVASKGGADEPPAWYLNLQADPTVEVQVKADKFTARARTASEEEKAG
                     IWPQMVKQWPAYDDYQAATDRPIPIVLLERA"
     gene            complement(5073..5915)
                     /locus_tag="PLJ64_07570"
     CDS             complement(5073..5915)
                     /locus_tag="PLJ64_07570"
                     /inference="COORDINATES: protein motif:HMM:NF015438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HNC93376.1"
                     /translation="MAGTVSWISIAPVKGMRIQSLNEVELTCDGVPGDRAFFLADEDG
                     AMVNGKRFGSLMEIIPAHDRESGRLSLEFPDGATVEGEVGLGEPEEVTFFGQPERARP
                     VTGDFSDAVSRHTGARLRLMAHPEGRPGVDRGKWGGVTLLGAASLESLREAGRDFNCA
                     EPSSEALPGGTASDPDPGPIDGRRFRMTFGVEGTRPYEEDDWTGREIRIGEALVRVAA
                     HAGRCAVTTRDPDTGVTDLKTLHFLKHSRDGVDSFEPLPFGVYGEVLEPGPVRIGDPV
                     GLAG"
     gene            complement(5939..6901)
                     /locus_tag="PLJ64_07575"
     CDS             complement(5939..6901)
                     /locus_tag="PLJ64_07575"
                     /inference="COORDINATES: protein motif:HMM:NF016535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNC93377.1"
                     /translation="MSDSLVVLLMVLVGVQFAAQAPVNGGLGRHTGGLVAALISFLVG
                     NVALLAIVAVAGQLGNFSSLGEVPLWQLAGGLIGATYVALAATTISRIGAGVIAAVTV
                     TGQLACSVVVDHFGWFGIEVHDVTGPRILGLVLLLAGAMAMLYSREEAGGGGASRAGR
                     QTGHGIGSMASGAENRERLVICAVIFLASLAVGIQHPLNSELANSIGDLPAGLTNFTV
                     GTILLALLVIVTGQAKALLGAREARPQYFLGGLIGLIVVVTSLSAVTLIGAAGLTAAL
                     VTGQMIGSLLLDRFGAFGLTRIPIDRVRLAAAAALLIGTFLATG"
     gene            complement(6921..>8791)
                     /locus_tag="PLJ64_07580"
     CDS             complement(6921..>8791)
                     /locus_tag="PLJ64_07580"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HNC93378.1"
                     /translation="MDFEVETGLDVDELMAQTPDREGLRAFMTEFELRAAMKRLEEAL
                     EDEEIPGVSEEAPAPAEVEVSKGGVGDLDEGPIAVELYGGWAATDGRKVVGGEATAEQ
                     LAGMVQGKPLIAHDIKAVGGQRVGLLAALEATEQGPVISHDTLVAAYLLEPSRRNYHL
                     DELAVQAGLAPSGIDGDDAQMSLIADEQDSGSIAPEIAATVWNLARVQRPKLEEAGLL
                     DLLEQVEQPLIRVLAAMERIGLKLDRERVAEMAAGLEDRIIELETEIHGLAGHEFTIG
                     SPQQVGQVLFEELGLTRKRKGKTGFSTDARVLAQIRQEHAIVALIEQWRELTKLKNTY
                     LEPLPAAIDPATGRVHTTFMQVAAPTGRISSIQPNLQSIPIRSEVGRPLRGCFVAEPG
                     NLLTSLDYSQVELRVLAHVADEEVLKGFFRAGEDVHSATAAQVFEIPPEEVDEAQRSK
                     AKMVNFGIVYGLTGFGLADRLNIPRSEGEEFVKRYHERFPAVTAFREQVVEEATETGF
                     VKTLLGRRRPLPELRSNQAQVRAQGVRLAMNTVVQGTAADIIKIAMIRSHQALLDEGL
                     SSRLVLQIHDELLFEGPAEEAEAVTELARREMVGAFDLDPPLEVDAGSGESWLAAK"
BASE COUNT         1421 a         3027 c         2790 g         1553 t
ORIGIN      
        1 tgttcaaccc gcccctccca ttcgaacaaa ctgatggttg ggaggggctc tgacttctgt
       61 gccccggttt tcattaccct ccaccggatg gcaaaacggg cgacaaagcg gggggacgaa
      121 cgaaccgatc tgaccggatc gcgcgacgtt ctcatctgtg gagccagttt tgccggactg
      181 atggtggccc gccagctggc cggtaccggc gcggacgtcc tggtcgtcga tcgctacgag
      241 atcggcgagc gccagacctc cgcctgtgcg atcccgactg cctggctgga ggagctgggg
      301 ctgatcgaat ccgagaagca gcgatttgac tcggtcctca tccacaccgc ccacggttcg
      361 atccgcttgc cggtcccctg gtccttctcc acctttgact acccgagctt ttgcgaaatg
      421 ctctgggccc agtgcgacgc cgagttcgaa accgccaagg tcaacggcag ggccaagacc
      481 cctgagggag accatgtgat cgacaccgat cggggcgaaa taaccgctcc gctggtggtc
      541 gattcgctcg gctggaagcg ggttctggct gccggcgccc actatcagcc gcccaaccag
      601 ccgctttctc gcggcctgga ggtccacccg ccgggtggca acgttgacct cgaagttttc
      661 atcaaccggc gctatgtccc ggccggttat ggctggtctt tcccggccga cgacgaagtc
      721 cgggtcgggg tcggttcctt cgatcctcac ttcccggtcc gcccgaccac cgacttgatc
      781 gccgacgacc tcaacgtcga aaagtcggca taccagggca actggatccc ccacaaactc
      841 cggcccgcga ccggtgacgg catcttcttc gccggggatt cggccggcca ctgcttcccc
      901 ctgaccgccg agggcatccg ccccgccctc tatttcggtg ccgcgctcgg catggaactt
      961 cgcgatgtag tggaaggccg caagtcacgc gaagaggccc tgacctgcta ccacgacttc
     1021 agcgccggcc accagtgggc ctttgactgg ctcctcaggg cccagaagct cctgccgaag
     1081 gtccaccccc gcctggtcaa accgaccctc gcggcctctt tcaaggccgg cggctcgggt
     1141 gactacgcct tcaacaagta cctcgacatc gccccaccgg aattcgccgc cctcgccaca
     1201 agggccctca aagaaaactc gcccgtaccc acctagcgtt ctacgaagta ggagccggat
     1261 tcgtggccgc ccgagaaata gcggctcgtg atcgtcttct tacgggtgaa gaactcgact
     1321 ccgtcggggc cgtgggcgtg gaggtcaccg aggaaggatg ccttccagcc cgagaagggg
     1381 aagaaggcaa ccggagcagc cacgccgatg ttgacgccga ccatgccgac ctcgacgtcg
     1441 tggcggaact tgcggaccgc cgaggcggat tcggtgaaga tcgaaacgcc gttgccgaag
     1501 ggactggcgt tgacgacctc gatcgcctcc tcgagagtgt cgacgttgac gatcgcgatg
     1561 accgggccga agacctcgtt ctcgatgatc gaatcggtcg gtttgacgtt gtcgaggatt
     1621 gtcggcccca cgtaggacga gccctcgggg gcatcgggca gcggcgtgcg cccgtcgacc
     1681 aggacctccg ccccttcctc gatcgccttg gcgacccagc cgtcgatgcg gtcgcgagcg
     1741 tcgcaggaaa tgaccgggcc gacgtcgatg ttctcgtcga gcccgttgcc gacatggagc
     1801 ttcttcgttt cctcgaccag cttcggaacc acgctgtcat ggcccgagcc cacggtgacg
     1861 atcaccgagc cggccatgca gcgctgaccg gcggcgccga agccggagcc gatcagtccg
     1921 ttgacggtcg gttccacccc cgcgtcgggg cagatcacca tgtggttctt ggccccgccg
     1981 agagcctgga cgcgcttgcc ggtctgggcc gcgcgctcgt agacgagctg ggcgaccggg
     2041 gccgatccaa cgaaggagat cgcgtcgatg tcggggtgat cgagcattcc ctcgacgacc
     2101 tcgcggctgc cgtttacgag gttgaccacc ccgtccggaa ggtcgagctc gtcgatgatt
     2161 ccgaagacct tctgctgggt catcggcacc tgctccgaag gcttcagcac gatcgtgttg
     2221 ccgcaggcga tcgcgaaggg caaaaaccag aacggcacca tggccgggaa attgaaggga
     2281 gcgatgatgc cgcagacccc gaccggctgg cggattgttt ccgcgtccac gccgcgggcc
     2341 acgtcctcga gcaccttgcc ctgcatcgtg gtcgggatcg cggtggccgc ctcgaccatc
     2401 tcgatcatcc ggccgacctc ggcggtggca tcgggcaggg tcttgcccat ctcggtgacc
     2461 acgcagcgag cgatttcgtc cttgcgcgcc tcgagcttct cgcgcagggt gaagagcatc
     2521 cgggcccggg cgatcgtgct tacgccgcgc cacgccggaa gagccttccg ggcggcgcga
     2581 accgcttcgt cgaggtgact ggcggtcgag aagggaaccc gggcgatgac ctctccgttg
     2641 gccggattga ccacgtcgtg aagctcggcc ccgccggctt cgacccactt gccgccaacg
     2701 taattctgaa gcgtggtggt ttcggtctcg gtgctgacgg tcatacggcc tcctcgggga
     2761 aaacgggtct ttctcaggct aacgcccgga gaccagaccg aaagtggaca taccgtgaag
     2821 tctcaacgcc aaactggacg ccacgtccag aacctggccg ggtcagccct tcgtgtagac
     2881 gatgctgtcg gtgccctttt ccaggatcag gttgccgtcg ctgttgacct cgtacttgac
     2941 ctcgccctga agcacctggg tgaccaggaa ctcgatctgg ttctggacct gttcgcagga
     3001 cttcctggtc agggcgatcg gcccgaagtc gatcgtggcc tcgtttaccg tcgcttcgcc
     3061 cgagccgctg ttgcagcccg cgaagatgct ggcctggccg tcgttgatct caagggtcgg
     3121 aggcttgact cccttgggca ggctggtcgc ggcgccctgg gttacgaggt tcatcgcctg
     3181 ccaggtcacg ccctcgaccg cggaagcctc ggagccctcg cccgaccctg aatcggagtc
     3241 cgacccgcag gccgctaccc cggcacccag cgccagaacc aacagaacca ccagaaatct
     3301 cctcaccttg gtcatgactg aagggtatat ccgatcgtga tgggtacggt cccaacgtga
     3361 cctccacgcc ggagcaggtt cagcgggtct acctgaccct gatgctgggc aacacgcttg
     3421 ctgcctcgtt catctggggg ataaacacga tcttccttct ggatgccggg ctttccaacc
     3481 tggaggcctt cgccgccaac gccttcttca cggccggcat ggtgatcttc gagattccga
     3541 ccggggtcgt cgccgacacg atcggccgcc gggtctccta catggccggg accctgaccc
     3601 tgatggccac cacgctgctc tacgtgatgc tctgggagat caacggaccc ttctgggcct
     3661 gggcggtcat atcactgctg ctcgggctcg gtttcacttt cttctccggc gccgtcgatg
     3721 cctggctggt tgacgcactg gacgaaacgg ggttcacggg agacctggaa cgggtctttg
     3781 gccggggcat gaccgtgacc ggaatcggga tgctggccgg ctcggtgatc ggcggctacg
     3841 tggcccaggt gaccagtctg ggggtgccgt tcgtgatgag agcggtcgtt ctgctggcga
     3901 tgttcttttt tgccgcatgg gcgatgaagg acctcggttt caagccgcac aaggccgaaa
     3961 gcaccttcgg cgaaatgaag tcgatcaccc ggaattcgat cgattacggc tggcgggtcc
     4021 cttcggtcaa gtggatcatg cttgcggctc ccttcaccgc cggggtcggg atttatgcct
     4081 tctacgcgct gcagccatac ctgctggagc tggtcggaga cccgcatgcc tacggcatcg
     4141 ccggcatcac cgctgcgctg gtagcgggct cccagatcat cggcggcttc ctcgccccga
     4201 ggatccgccg ccgtttcagg ttgcggacat ccgcgctgct cctggcggtc tctgtcggtg
     4261 cggtgacgct cgccctgatc ggggtgatcg agcagttctg ggtcgcggtc ggactgatcg
     4321 ccacctgggc catgctctcg tcggcggcga ccccgatccg gcagtcctac atcaacggga
     4381 tgatcccctc ccgccagcgg gcgacgatcc tttccttcga ctcgatgctg ggatcgaccg
     4441 gcggggtcgt ggtccaaccc gcgctcggcc gggtcgccga cacctccggg tacgcgacct
     4501 cctacctcta cggcgcgatg atcaccgtct ttgcccttcc gttcatcctc ctgtcccggc
     4561 accagcaccc gccggccgac cgcgcgaccg gaactccgga ggcggaaggc acggtcgagg
     4621 ccgggcccga atctgatcgg tgaggttcag gccctttcga gcaggacgat cgggatcggc
     4681 cggtcggttg ccgcctggta atcgtcgtag gccggccact gtttgaccat ctgcggccag
     4741 atcccggcct tttcttcctc gctcgcggtc ctcgcccggg ccgtgaactt gtccgccttc
     4801 acctggacct cgaccgtcgg gtcggcctgc aggttcaggt accaggccgg cggttcgtcc
     4861 gccccgccct tcgaggcgac gatcgcgtag gcatccccga ccgggtcgta gatcagcggc
     4921 gtggtccgct tctcaccgga ttttcgcccc gtggtggtga gcagcagggt gtgagtgccc
     4981 tgccagtcat gaccctcttc accgccggtg gcctgataac gatcaacgtg ctgctgtccg
     5041 aacaacatga tcccaaccta accgcggacc cctcacccgg caagaccgac cgggtcaccg
     5101 atccgcaccg gccccggttc cagaacctcg ccatagacgc cgaagggcag cggctcaaac
     5161 gaatcgaccc cgtcacgcga gtgcttgagg aaatgcaggg tcttcaagtc ggtcaccccg
     5221 gtgtcggggt cgcgggtggt taccgcgcaa cggccggcgt gggccgcgac cctgaccagg
     5281 gcttcgccga tccgaatttc ccggccggtc cagtcgtcct cctcataagg cctggttcct
     5341 tcgacgccaa aggtcatgcg gaagcgccgc ccgtcgatcg gccccgggtc gggatcgctc
     5401 gcagttccgc ccggcagcgc ttcgcttgaa ggctccgcgc agttgaaatc ccgccccgcc
     5461 tcgcgcaggc tctccagcga agccgcaccg agcagggtca cgccgcccca tttgccgcga
     5521 tccaccccgg gccgcccttc cgggtgggcc atcagccgca gcctggcccc agtgtgcctc
     5581 gagaccgcgt cggagaaatc accggtgacc ggccgggccc gctcgggctg gccgaagaaa
     5641 gtgacctcct cgggctcacc gagcccgacc tcgccctcaa cggtcgcgcc gtcgggaaac
     5701 tcaaggctca gcctgcccga ttcccggtcg tgtgcgggga tgatttccat gagcgaaccg
     5761 aagcgcttgc cgttgaccat cgcgccgtct tcatcggcga ggaagaaggc ccggtcgccg
     5821 gggactccgt cgcaggtcaa ctcgacctcg ttcagcgact ggatgcgcat ccccttgacc
     5881 ggggcgatcg atatccagga gacggttccg gccacgcccg gatcctctca tgcccggatc
     5941 agccggtggc gaggaaggtg ccgatcagca gggccgccgc cgcggccagc cggacgcggt
     6001 cgatcgggat gcgggtcagg ccgaaggcgc caaaccggtc gagcagcagc gagccgatca
     6061 tctggccggt gaccagggcc gcggtcaggc cggccgcacc gatcaaggtc acggctgaaa
     6121 gcgaagtgac gaccacgatc aggccgatca ggccgccaag gaagtactgc ggccgggcct
     6181 cgcgggcccc gagcagcgcc tttgcctgcc cggtgacgat caccagcaac gccagcagga
     6241 tcgtcccgac cgtgaagttg gtcagcccgg cgggcagatc gccgatcgaa ttcgccagct
     6301 cggagttgag cggatgctgg attccgaccg ccagggaggc gaggaagatc accgcgcaga
     6361 tgaccagacg ctcgcggttc tcggcgccgc tggccatcga cccgatcccg tgacccgtct
     6421 gccggccggc ccgggaagcc cctccgccgc ctgcctcctc ccggctgtag agcatcgcca
     6481 ttgccccggc caggagcagc acgagaccga ggattcgcgg cccggtcacg tcgtggactt
     6541 cgatcccgaa ccacccgaag tgatcgacca ccaccgaaca ggcgagttga ccggtaaccg
     6601 tgaccgcggc gattacgccg gcaccgatcc gcgagatcgt ggtcgccgcg agggccacat
     6661 aggtggcacc gatgagcccg ccggccagct gccagagcgg tacctcaccg agggaagaga
     6721 agttgccgag ctggccggcg accgcgacga tggccagcag ggccacgttg ccgaccagga
     6781 aggagatcag agccgcgacc agcccgccag tgtggcggcc gagcccgccg ttgaccgggg
     6841 cctgggcggc aaactggacc ccgaccagga ccatcagcag gacgaccagg gaatcgctca
     6901 tgccggaatc cgggctgccg ctacttggcc gcgagccagc tctcgccgga gccggcgtcg
     6961 acctccagcg gcgggtcaag gtcgaaggcg ccgaccattt cccgccgggc cagttcggtg
     7021 accgcttcgg cttcctccgc cgggccttcg aaaagcagtt cgtcgtggat ctgcagcacg
     7081 aggcggctcg agagaccttc gtcgagaagc gcctggtggc tgcggatcat cgcgatcttg
     7141 ataatgtcgg ccgcggtgcc ctgcaccacg gtgttcatcg ccagccgcac cccctgggcc
     7201 cggacctggg cctggtttga acgcaactcc ggcaacggtc gccgccggcc gagcagggtc
     7261 ttgacgaaac cggtctcggt cgcctcctcc accacctgct cccggaaggc ggtgaccgcc
     7321 gggaaccttt cgtggtagcg cttgacgaat tcctcccctt ccgagcgggg gatgttcagg
     7381 cggtcggcca ggccgaagcc ggtcaggccg tagacgatgc cgaagttgac catctttgcc
     7441 ttcgaccgct gcgcctcgtc gacctcttcc gggggaatct cgaagacctg ggccgcggtc
     7501 gccgagtgga catcctcccc ggcccggaag aatcccttca gcacttcctc gtcggcgaca
     7561 tgggccagca ccctgagctc cacctggctg tagtcgagcg aggtgagcag gttgcccggc
     7621 tcggcgacga agcagccccg gagcgggcgg ccgacctcgg aacggatcgg gatgctctgc
     7681 agattgggct ggatcgagga gatccgccca gtcggggcgg cgacctgcat gaaggtcgtg
     7741 tgaacgcgcc cggtcgccgg gtcgattgcc gccggcagcg gctccaggta ggtgttcttg
     7801 agcttggtca gctcgcgcca ctgctcgatc agggcgacga tcgcgtgttc ctgccggatc
     7861 tgggccagca cccttgcgtc ggtcgagaaa ccggtcttgc ccttccgctt gcgggtcagg
     7921 cccagctcct cgaagagcac ctggcccacc tgctgcggcg agccgatcgt gaactcgtgg
     7981 ccggccagtc cgtggatctc ggtttccagc tcgattatcc ggtcctcgag accggcggcc
     8041 atttcggcga cccgttcacg atcgagtttc agcccgatcc gctccatggc cgcgaggacc
     8101 cggatcaagg gctgttccac ctgctccagc aaatcgagca ggccggcctc ctcgagcttc
     8161 ggcctctgga cccgggccag gttccacacc gtggcggcga tctccggggc gatcgatccg
     8221 gagtcctgtt cgtcggcgat cagcgacatc tgggcgtcgt caccgtcgat gccggaaggt
     8281 gccagaccgg cctgcaccgc gagttcgtcg aggtggtagt tccgcctcga gggttcgagc
     8341 agataggcgg cgaccagtgt gtcgtgactg atcaccggcc cctgttcggt cgcttcgagc
     8401 gccgccagca acccaacccg ctggcccccg actgccttga tgtcatgcgc tatcaacggc
     8461 ttcccctgga ccatgccggc cagttgttcg gcggtcgcct cgccgccaac gactttccgg
     8521 ccgtcggtcg ccgcccatcc gccgtagagc tcgaccgcga tcggcccctc gtcgaggtct
     8581 ccgacaccgc ctttcgagac ttcgacttcg gccggggccg gagcttcctc ggaaacgccg
     8641 gggatctctt cgtcctccag ggcttcttcg agccgtttca tcgccgccct gagctcaaac
     8701 tcggtcatga aggcgcgaag gccctcccgg tccggggtct gggccatcag ctcatcgacg
     8761 tccagccccg tttcaacttc gaaatccatc g
//
LOCUS       DAOQKN010000096         8682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_3880_length_8682_cov_4.974730, whole genome shotgun sequence.
ACCESSION   DAOQKN010000096 DAOQKN010000000
VERSION     DAOQKN010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 8682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8682
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3880_length_8682_cov_4.974730"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1342)
                     /locus_tag="PLJ64_07585"
     CDS             complement(<1..1342)
                     /locus_tag="PLJ64_07585"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HNC93379.1"
                     /translation="MKFRLMFGLFAAASLFALPNQAGAEISMNPMPGNPLTDTGQGGM
                     VRPVDLNDDGLDDLAIGSATAATGGVRIRIAGADGAWSPKPTLLAGSPVGDIVPADFD
                     QDGNEDLLVQISFPGLSANDQYRILSGHGDGTFAAPTSVAVPSGKVVISLPTGHNILP
                     TDIDGDGHLDLFIGLTRGQIAVARGHGDGSFDPLGVSASFPDTPTADSEGIGNVVAGD
                     YNADGRTDYAFTLLGNSAGTSTQANSGFYVMYGQGGASFSEVTRIGMADPQGPITSAF
                     SDDLNGDGSDDLIIAAVSPETTSTRFILYPGGNGGFATLPTTLFSLPVITPRVSGADF
                     NRDGNLDLAWVERADSGSPRANNLVLATGNGSGGFTRLSGAFDLNFGATQDGVSTASP
                     GDFNGDGSPDFAASFYSSDCAVNACGVSILMNRAVIGPATSSVDFGTVLQGTATA"
     gene            complement(1417..2604)
                     /locus_tag="PLJ64_07590"
     CDS             complement(1417..2604)
                     /locus_tag="PLJ64_07590"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HNC93380.1"
                     /translation="MSRGHLAAVLVTLTCLLAIPASASAVEATADGNSVQLSDTPLEA
                     AYTFDPGQVAFGDVPVGSTASQLITLTNTGGSPALIRGVFAGKVGTDWLSFGISQDTC
                     SPVPPGITRELPAGQSCSYLLEFTPSQRGGYAEITGVVTEGEVHFIPVGGNGTEPAGE
                     FDTGSFDFGSSPVGPSSNRPKHTFNLSSTGLDPVTVQSLSIGGPDQDAFELADPAVCS
                     APIPVNEKCQITVTFDPESGSPGPRAATLTALTTGGVVEVALTGNATAPLTYRAKMQI
                     KFPKKAVVGRSARFKVTLTNTGTGPIAGAALEYKARQGKSGKAVVSKSVKVPALQSGR
                     KTAMTVTVKLPKNRLSRGKGKGLLVNFELVHQGLTLAARRGTTRLDFGQAKSRRPRDV
                     SDT"
     gene            2760..3251
                     /locus_tag="PLJ64_07595"
     CDS             2760..3251
                     /locus_tag="PLJ64_07595"
                     /inference="COORDINATES: protein motif:HMM:NF012479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNC93381.1"
                     /translation="MSDSESPVVLTGTMNRLNGTEQDLGEYLGQVTLVVNTASKCGLT
                     PQFEALQAVYSDKKDEGFTVLGFPANDFMEQEPGSADEIAEFCQINYGVEFPMFEKST
                     VVGDEANPLFAELAAQSEQPEWNFAKYLIDREGRLVRKFPAPTTPDDPEVIEAIEAEL
                     ASE"
     gene            complement(3261..4109)
                     /locus_tag="PLJ64_07600"
     CDS             complement(3261..4109)
                     /locus_tag="PLJ64_07600"
                     /inference="COORDINATES: protein motif:HMM:NF025252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2470 domain-containing protein"
                     /protein_id="HNC93382.1"
                     /translation="MSTETKNERDLKAVIGVHDADPDTAVPDVAPIAFGGNRRSAAEE
                     SRTILANATHGTLGSLSEDGTPWASLVTFGVMDDGSPVFCLSTLAEHARNLENSRQAS
                     LMVSDPFHTGEILAGGRVTLAGKVKRSDEHPDETSARAAHLEAVPSAAMYADFNDFAF
                     WILEVDRVRWVGGYGRMDSASAEEYSQAEPDPVQPQVPDAIAHLNADHPDALLLMARA
                     FTKYQDATEAKCERADRYGLDLQLTTPEGDKAARVSFTDRIDKPGGLRAATVDLARRS
                     RQKLGS"
     gene            4278..5186
                     /locus_tag="PLJ64_07605"
     CDS             4278..5186
                     /locus_tag="PLJ64_07605"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNC93383.1"
                     /translation="MTTAAARPQFRFSRIFGALLLLAAVLAGFAFVTPQADAAKQKRV
                     VAISPFAAEAIVQMGAKKNLVAVGQTIGASDHHNKLIRGIPKLKLSHPNGPNLEVMAK
                     LKPDVVFSSERWAAGTKALNRLGIRVVYADPEKLGQVKPSVSKIGRVLGKGRQAAALN
                     RRISKSFSKSTRPSTKHPKVLVVLGIGNTAMAFLHNSWGGQLVDKAQANLVTGGAKAD
                     GGFARLSDEVVVAQNPDIIIVVPHGNSDDIDQVYDYIMNNDAWQSTTAVEKGKVFVSI
                     HNELLQAGTNVGSVISGLRTKWLKNW"
     gene            5267..6223
                     /locus_tag="PLJ64_07610"
     CDS             5267..6223
                     /locus_tag="PLJ64_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNC93384.1"
                     /translation="MLLALVAAACASLAYGAVHIPLGEVIDTLLGQKVPEASRQIIEV
                     LRLPRTVEAIVVGAALGVAGACLQGALNNPLASPDVIGVTAGAGFGAMLVLLFSPASV
                     ALLPISALAFGLIAAAMVFLIGFTGRGGGNITRIILAGIAIAALFSAATAGLLSAYPD
                     RVPNAVFFLVGGLTSSDGWDTLETIWPYLAVGFVIAAFMIRPLDRLMLGDDVAASLGS
                     RPRLIRLTAAFAAALLASAAAAIAGLLAFLGLVIPHLVRLLGGTRNHTFVIPVSALAG
                     AALLTAADTVARTIAAPIEMPVGPFLVVLGVPLFLWLLRKAV"
     gene            6223..7047
                     /locus_tag="PLJ64_07615"
     CDS             6223..7047
                     /locus_tag="PLJ64_07615"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC93385.1"
                     /translation="MAPGRRTAAFERKAPLLEAKEVQVTIGGEEILESASLDLRRGEL
                     LAIVGPNGAGKSTFARAFCGLRRPQGGEVRWMGVPLTELRGRKLAGVRAYVPQRAQVP
                     TGVTVQAAVNIGRSTHLKPLQGLSGEDREAVRESIARARLEGLEDRDLSTLSGGEMQR
                     VQIAVALAQGAPVLVADEPTAQLDLGATAAISKMLRELVSGGMGVVLIVHDLALAAAI
                     ADRVVVMSHGRTIATGTPEAVLTGQLISEVWGVQAELVHREGRSALHVEWLDQHQT"
     gene            7143..8438
                     /locus_tag="PLJ64_07620"
     CDS             7143..8438
                     /locus_tag="PLJ64_07620"
                     /inference="COORDINATES: protein motif:HMM:NF016129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HtaA domain-containing protein"
                     /protein_id="HNC93386.1"
                     /translation="MSSSLSAARRTMAALTVLATAILLLIGLNPGQAAAVETTKGPIV
                     FSMTSGKAGKVSAISPASVVKRFGKKGAKVSTAAKNGSFGEKVASGLQAAGGLRLANG
                     KRSVALTGLKLTIDRKLAVVRGNVAGKKNIVVFTAAGKGTLSADPGSVKFTGGKLRFP
                     ASVANLVRNRLKLKTAPTGPIGTLFVNAFLDTTVPVDPCELDPDAEGCPVIDPYLTEC
                     NVAATSKATGSLPAAAPLPTLTNPQAITGLTTVGWGIKSSFRNYMVSPAAAGSIHARD
                     GATATGSAPAYSGFDWTFVDGQYADNGTPNDPSDDQAILNLSGTVVLCATGHSFRVAI
                     SDPTIVVDGANSRIIADVDSNLTGVWTPAQRIDVATLNLAGGTTSLLSGTLGTKWENV
                     PAAMTENGGKAICGVTEPQCPYSTGTAMDPVTVEARAGI"
BASE COUNT         1442 a         2853 c         2907 g         1480 t
ORIGIN      
        1 cggccgtcgc cgttccctgc agcacggtgc cgaaatcgac cgacgaggtg gccgggccga
       61 tcaccgcccg gttcatcagg atcgaaactc cgcatgcgtt caccgcacag tccgagctgt
      121 agaagctggc cgcgaaatcg ggcgatccgt caccgttgaa gtcgcccggg ctggcggtgg
      181 aaacaccgtc ctgggtcgca ccgaagttga ggtcgaaggc gccggaaagc cgggtgaacc
      241 cgccgctgcc gttgccggtg gcgaggacca ggttgtttgc tcgcgggctg ccgctgtcgg
      301 ctcgctcgac ccaggccagg tccagattcc cgtcccggtt gaagtcggcg ccggagactc
      361 ggggtgtgat gaccggcagt gagaagagcg ttgtcggcag ggtggcgaat ccgccgttgc
      421 cgccggggta gaggatgaaa cgggtcgagg tggtttcggg cgacacggcc gcgatgatca
      481 ggtcgtcgct gccgtcaccg ttcaggtcat ccgagaaggc actggtgatc ggcccctgcg
      541 gatcggccat gccgatcctg gtcacttcgc tgaaactggc gccaccctgt ccgtacatca
      601 cgtagaagcc cgaattggcc tgggtcgagg tgcccgcaga gttgccgagc agggtgaatg
      661 cgtaatcggt ccggccgtcc gcgttgtaat ccccggcgac gacgttgccg atgccctccg
      721 agtcagccgt gggggtgtca ggaaagcttg ccgaaacgcc gagcgggtcg aaactgccgt
      781 cgccgtggcc gcgggcgacc gcgatctggc cgcgagtcag gccgatgaac agatccaggt
      841 ggccgtcacc gtcgatatcg gtcgggagaa tgttgtgacc cgtgggcagg gagatgacga
      901 ccttgccgga cggcacggca acgctggtcg gcgcggcgaa agtgccgtcg ccgtgaccgg
      961 aaaggatccg gtactgatca ttggccgaca ggccggggaa gctgatctga accagaaggt
     1021 cctcattgcc gtcttggtca aaatcagccg gaacgatgtc accgaccggt gagccggcca
     1081 gcagggtcgg tttcgggctc caggcgccgt cggcgccggc aatccggatg cggaccccgc
     1141 ctgtggcggc ggtggcagat ccaatcgcca ggtcgtccag gccatcgtcg ttgaggtcga
     1201 ccggccggac cattcccccc tgcccggtgt cggtcagggg attccccggc atcgggttca
     1261 tcgagatctc ggcccccgcc tgattcggca aggcaaaaag cgatgcggcc gcgaacagcc
     1321 cgaacatcag acgaaacttc atggtgttct ccctcaacct tctggttggc tttcccaagc
     1381 aggagtgatt gtgacggacc ttgcggtcgc gggcttctag gtgtcggaga cgtcccgcgg
     1441 ccggcggctc ttggcctgac cgaaatcgag gcgggtcgtg cctcttcggg cggccagggt
     1501 cagaccctgg tgaaccagct cgaagttgac cagcagtccc ttccccttgc cgcgggagag
     1561 ccggtttttc ggcagcttga ctgtgaccgt catcgcggtc ttgcggccgc tctgcaaggc
     1621 agggaccttc acggatttgc tgaccacggc cttgccgctc ttgccctgcc tggccttgta
     1681 ctcgagtgcg gcgccggcga tcggcccggt gccggtgttg gtcagagtca ccttgaatct
     1741 ggccgacctg ccgaccacag ccttcttcgg gaacttgatc tgcatcttcg ccctgtaggt
     1801 gaggggcgcg gtggcgttgc cggtcagggc gacctcgacc acgccacccg tggtcagggc
     1861 ggtcagggtg gcggcgcggg gaccgggact gccggactcc ggatcgaagg tgaccgtgat
     1921 ctggcatttc tcgttgaccg ggatcggggc cgagcagacg gcgggatcgg ccagctcgaa
     1981 ggcgtcctgg tccggccccc cgatggaaag cgactggacg gtgaccgggt cgaggccggt
     2041 cgaggaaagg ttgaaggtgt gtttggggcg gttcgacgag gggccgaccg gagagctgcc
     2101 gaagtcgaag cttccggtgt cgaactcgcc ggccggctcg gtgccgttgc ccccgaccgg
     2161 aatgaagtga acttcgcctt cggtcacgac cccggtgatc tcggcatatc cgccccgctg
     2221 tgagggagtg aattcgagca ggtaggaaca ggactggccg gcgggaagct cgcgggtgat
     2281 tccgggcggg acggggctgc atgtgtcctg ggaaatgccg aatgaaagcc agtcagtgcc
     2341 cacttttccg gcaaagaccc ccctgatcag ggccgggctc ccaccggtat tggtcagagt
     2401 gatcaactgt gacgcggtgg agccgaccgg aacgtcgccg aaggcgacct ggccggggtc
     2461 gaatgtgtag gccgcctcca ggggggtgtc ggagagctga acggagttgc cgtcggccgt
     2521 ggcctctacc gccgaagcgg atgccgggat ggcgagcagg caggtcaacg tcaccagaac
     2581 tgcggccaga tgaccccttg acatccctta cccccaattg ctcgttacct gaaaggaaga
     2641 tcccctctgc cgaccatatt cctttttccg gtccggcgag ccggcctagt tcttcccggt
     2701 tatctgggaa ggatggaaag gctgaaagtt gccggtgcgg agcgggtacg atcccgctca
     2761 tgagcgatag cgagagcccg gtcgtcctga ccggcaccat gaaccggctg aacggcaccg
     2821 aacaggatct gggggagtac ctgggtcagg tgaccctggt cgtcaatacc gccagcaagt
     2881 gcgggctgac cccgcagttc gaggccctgc aggcggtcta ttccgacaag aaggacgagg
     2941 ggttcacggt gctcggtttc ccggccaacg acttcatgga gcaggaaccc ggcagtgccg
     3001 acgagatcgc cgagttctgc cagatcaact acggggtcga gttcccgatg ttcgagaagt
     3061 cgaccgtggt cggggacgag gcgaacccgc tctttgccga actggccgca cagtccgaac
     3121 agccggaatg gaacttcgcc aagtacctga tcgaccgcga aggccggctg gtcaggaagt
     3181 ttccggcacc gaccaccccg gacgacccgg aagtgatcga ggcgatcgaa gccgagctgg
     3241 cctccgaata gggggccggc ctagctgccg agcttctggc gcgagcgccg cgcgaggtcg
     3301 acggtcgccg cgcgcaggcc gccgggcttg tcgatccggt cggtgaaact gacccgggcc
     3361 gccttgtcgc cctcgggagt ggtgagctga aggtccagtc cgtagcggtc ggcccgttcg
     3421 cacttcgcct cggtcgcgtc ctggtatttg gtgaaggccc tggccatcag caggagcgca
     3481 tcggggtgat cggcgttcag atgggcgatc gcatcgggca cctggggctg gaccggatcc
     3541 ggctcggcct ggctgtactc ctcggccgac gctgaatcca tgcggccgta gccaccgacc
     3601 cagcgcaccc ggtcgacctc gaggatccag aaggcgaagt cattgaagtc cgcgtacatg
     3661 gcggcggaag ggacggcttc gaggtgtgcg gcccgggccg aagtttcgtc ggggtgctcg
     3721 tccgaacgct tgaccttgcc agccagggtt acccggccac cggccaggat ctcgcccgtg
     3781 tgaaacgggt cgctgaccat cagcgaagcc tgccggctgt tctcgaggtt gcgggcgtgc
     3841 tcggccaggg tcgagaggca gaagaccggg ctgccgtcat ccatgacccc gaaggtgacc
     3901 agcgaagccc agggggtgcc gtcctcgctg agcgatccga gcgtgccgtg agttgcgttg
     3961 gcgaggatcg ttcgcgactc ctcggcggcg ctgcggcggt tgcctccgaa ggcgatcggg
     4021 gcgacgtccg gaaccgcggt gtccgggtca gcgtcgtgaa ctccgatgac ggccttcagg
     4081 tcacgctcgt tcttggtttc ggtcgacacc gctgctcgct ttcggttcag gttgaggggt
     4141 tggggaggac ggacgggaag aagttagggt accctattcc taccgcagcg gcgggattca
     4201 cggctggtcc aataccttcg cctccgcgct gtgttccaag gttacttgcc tacccctcga
     4261 aggagtccag cccgcagatg acgaccgccg ccgcacgccc ccagtttcga ttcagccgga
     4321 ttttcggggc gctcctgctg ctcgctgccg tcctcgccgg attcgccttc gtcactccgc
     4381 aggctgacgc cgccaaacag aaacgggtcg tggcgatcag ccccttcgcc gccgaggcaa
     4441 tcgttcagat gggcgcgaag aagaacctgg tcgcggtcgg ccagacgatc ggcgcctccg
     4501 atcaccacaa caaactgatc cgcggcatcc cgaagctcaa actgagccac ccgaacgggc
     4561 cgaacctcga ggtgatggcg aagctgaagc ccgacgtcgt cttcagttcg gagcgctggg
     4621 ctgccggcac caaggctctg aaccggctgg gcatccgggt ggtctatgcg gacccggaaa
     4681 agctcggcca ggtcaagccg tccgtgagca agatcggccg ggttctcggc aagggcaggc
     4741 aggcggccgc cctgaaccgg cggatcagca agagcttcag caagtcaacc cggccctcga
     4801 ccaaacaccc gaaggtgctg gtcgtgctcg gcatcggcaa caccgcgatg gccttcctcc
     4861 acaactcgtg gggcgggcag ctggttgaca aggcccaggc caacctggtc accggcgggg
     4921 cgaaggctga cggaggcttt gcccggctct ccgacgaggt ggtcgtggcc cagaacccgg
     4981 acatcatcat cgtggtgcct cacggcaaca gcgatgacat cgatcaggtc tacgactaca
     5041 taatgaacaa cgatgcctgg cagtccacga ccgcggtcga gaaggggaag gtcttcgttt
     5101 cgatccacaa cgagctgctc caggccggca ccaatgtcgg ttcagtgatc agcgggctgc
     5161 ggaccaagtg gctgaaaaac tggtgaacgg ccggcccgtc cggtcctgat cccgaaaccg
     5221 ggggagtgag ctgacttcag ccagaccgat attcgtcgcg gccggaatgc tcctcgccct
     5281 ggtcgcggcg gcctgcgctt cgcttgcgta tggcgcggtc cacatcccgc tcggcgaggt
     5341 gatcgacacc ctgctgggcc agaaggtccc cgaggccagc cggcagatca tcgaagtgct
     5401 gcggttgccc cgcacggtcg aggcgatcgt ggtcggggcg gcgctcggcg tggccggggc
     5461 ctgcctgcag ggagcactca acaacccgct cgcctcgccg gacgtgatcg gggtcaccgc
     5521 cggagcgggt ttcggcgcga tgctggtcct cctgttcagc ccggccagcg tggcgctgct
     5581 gccgatcagc gcccttgcct tcggcctgat cgccgccgcc atggttttcc tgatcggctt
     5641 caccgggcgc ggcggcggca acatcacccg gatcatcctc gccggcatcg cgatcgccgc
     5701 cctgttctcg gcggcgaccg cggggcttct gtcggcctat ccggaccggg tgccgaacgc
     5761 cgtcttcttt ctggtcggcg gcctgacctc ctcggacggc tgggacacgc tcgagacgat
     5821 ctggccctac ctggcggtgg gcttcgtgat cgccgccttc atgatccggc cgctggaccg
     5881 gctgatgctc ggcgatgacg ttgccgcctc gctcggctcc cggccccgcc tgatccgcct
     5941 caccgccgcc ttcgccgccg ccctgctcgc ctccgccgct gccgcgatcg ccggcctgct
     6001 cgccttcctc ggcctggtca tcccgcatct ggtccgtctg ctcggcggga ccaggaacca
     6061 caccttcgtg atcccggttt cggccctggc cggggcggcc cttttgaccg cggcagacac
     6121 cgttgcccgg accatcgccg caccgatcga gatgccggtc ggtcccttcc tcgtggtgct
     6181 gggcgtgccc ctcttcctct ggctcttgag gaaggcggtc tgatggcccc cggcagaaga
     6241 accgccgcct tcgagcgcaa ggcaccgctg ctcgaggcga aagaggtcca ggtcacgatc
     6301 ggcggcgagg agatcctcga atcggccagc ctggatctcc ggcggggcga gctgctcgcg
     6361 atcgtcgggc ccaacggcgc cggcaagtcg accttcgccc gcgccttctg cgggctgcgg
     6421 cggccgcagg ggggagaggt gcgctggatg ggggtgccgt tgaccgaact tcgcggccgc
     6481 aagctcgccg gggtccgggc ctatgtgccg cagcgggccc aggtccccac cggggtcacc
     6541 gtgcaggctg ccgtcaacat cggccgctcg acccatctca agccgctcca gggcctttcc
     6601 ggagaggatc gcgaagcggt cagagagtcg atcgcccggg cccggctgga aggcttggag
     6661 gaccgtgacc tcagcaccct ttccgggggc gagatgcagc gggtccagat cgcggtcgct
     6721 ctggcccagg gtgccccggt actggtcgcc gacgaaccga ccgcccagct tgacctcggg
     6781 gctacggccg cgatctcgaa gatgctccgc gaactggtct ccggcggcat gggcgtggtg
     6841 ctgatcgttc atgacctcgc cctggcggcg gcgatcgccg accgggttgt ggtcatgtct
     6901 cacggaagga cgatcgcaac tggcacccct gaagcggtgt taacgggtca attgatcagc
     6961 gaagtatggg gagtgcaggc cgaactggtt caccgcgaag gccgctccgc gctccacgtc
     7021 gaatggctgg atcagcacca gacatgacta atccgatcct gttagtgtga attcataagg
     7081 caaccctaat tcgttttcca gtttccaccc ctcagcgaag tttccaagga gattttcaac
     7141 acatgagttc gtccctctca gccgctcgcc gcaccatggc tgccctcacc gtcctcgcca
     7201 cggcaatcct gctcctgatc ggtctcaacc ctggccaggc cgccgcggtc gagaccacca
     7261 agggcccgat tgtcttctcc atgacctccg gcaaggccgg caaggtctcg gccatttcac
     7321 cggccagtgt cgtaaagcgg ttcggcaaaa agggcgcgaa ggtcagtacc gccgcgaaga
     7381 acggcagctt cggtgaaaag gtcgcgagcg gcctccaggc ggccggcggt ctccgcctcg
     7441 ccaacggcaa gcggagcgtg gcgctcaccg gcctcaagct gacgatcgac aggaaactgg
     7501 ccgtcgtccg cggcaacgtc gcgggcaaaa agaacattgt ggtcttcacc gcggccggaa
     7561 aaggcaccct gagcgccgac ccgggcagcg tcaagttcac tggcggcaag ctcaggttcc
     7621 ccgccagcgt tgccaacctc gtcaggaaca ggctcaaact gaagacggcg ccgacgggac
     7681 cgatcggcac gctcttcgtg aacgccttcc tggacacgac cgtcccggtc gacccctgtg
     7741 agctcgaccc ggacgccgag ggttgcccgg tgatcgatcc gtacctgacc gaatgcaacg
     7801 tggcggcgac cagcaaggcg accggttcgc tccccgctgc cgccccgctg ccgaccctga
     7861 ccaacccaca ggccatcacc ggtctgacca cggtcggctg gggcatcaag tcgagcttcc
     7921 gcaactacat ggtcagcccg gccgcggcag gcagcatcca cgccagggac ggtgccaccg
     7981 ccaccggctc ggccccggct tactccggct ttgactggac tttcgtggac ggccagtacg
     8041 ccgacaacgg caccccgaac gatccgtcgg atgaccaggc gatcctcaac ctgagcggaa
     8101 ccgtcgtgct ctgcgcgacc ggccacagct tccgcgtcgc gatctccgat ccgacgatcg
     8161 tcgtcgacgg ggcgaattcg cggatcatcg ccgatgtcga ttcgaacctg accggggtct
     8221 ggactccggc ccagcggatc gacgtcgcca cgctgaacct ggccggcggc acgacctcac
     8281 tgctcagcgg caccctgggc accaagtggg agaacgttcc cgccgcgatg accgaaaacg
     8341 gcggcaaggc gatttgcggc gtcaccgaac cgcaatgccc ctacagcacc ggcaccgcga
     8401 tggacccggt tacggtcgag gccagggccg gcatctaacc ttcgggaact tcgctcgatt
     8461 cgctggagac cgacaaggga ccgattctga gtcgcaggac ctcatattcc gtcactgatg
     8521 tgacctccac gagggcggcc ctgtacgggt tcgccctcgt cgcggttctg ctcgcggttt
     8581 tcgtcggctt cggttcccga ccggcgttgg ccggggccgc gaccctcgag ccctgcgagg
     8641 cgaccaatcc gaccgggccg atcgtcgaac tcgacggcca gc
//
LOCUS       DAOQKN010000097         8648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_3909_length_8648_cov_6.030723, whole genome shotgun sequence.
ACCESSION   DAOQKN010000097 DAOQKN010000000
VERSION     DAOQKN010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 8648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8648
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3909_length_8648_cov_6.030723"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1727)
                     /gene="acnA"
                     /locus_tag="PLJ64_07625"
     CDS             complement(<1..1727)
                     /gene="acnA"
                     /locus_tag="PLJ64_07625"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HNC93387.1"
                     /translation="MSSNSKDSFSSKDTLETGGRSYEIFRLDALQKKFDVARLPYSIK
                     VLLENVLRLEDGVSVSAADVEAIASWDAKAEPSDEIPFQPARVLMQDFTGVPAVVDLA
                     AMRDAMADIGGEVSAINPLVDVDLVIDHSVQVDAFGNGMAFEINAEKEFERNLERYEF
                     LKWGQGSFNNFRVVPPATGICHQVNLEYIAQVVYSRENDGVLQAYPDTLVGTDSHTTM
                     VNGLGVLGWGVGGIEAEAAMLGQPVSMLLPQVIGFKLHGKLPAAATATDLVLTVTEML
                     RERGVVGKFVEFYGPGLSNLPLADRATLGNMSPEFGSTCAIFPPDAETLKYLELTGRP
                     TETIELVDAYARLQGTFHTADSGDPVFSDTLELELDSVVPSIAGPKRPQDRVSLSDAK
                     PAFLEALCEFDEDASNQLSAYDEALEESFPASDPPGEDHVTSDLGKPAGAPTRASTRV
                     AEQRCSEDMIGVTLEDGTSFDLDHGRVVIAAITSCTNTSNPYVMVGAGLLARNALAKG
                     LSRKPWVKTSLAPGSTVVTDYLDNAGLTQYLDQLQFNLVGYGCTTCIGNSGPLAPEIS
                     AAIDEKDLVV"
     gene            complement(1753..2553)
                     /locus_tag="PLJ64_07630"
     CDS             complement(1753..2553)
                     /locus_tag="PLJ64_07630"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC93388.1"
                     /translation="MDIAGKKVLLTGATGGIGRRIASTLAAAGAEVIVSGRSAEALTE
                     LVDSLPGSGHSVLAADLGLVGAAQELANQAGAVDMLVANAALPSTGRITDLTDEQVAR
                     MLRINLESPILLAQALLPGMLERGQGKLVMIGSLAGKAGSPRSSIYNATKFGLRGFAF
                     GLAADLAGSPVSITLVAPGFVREAGMFADSGADAPPGLGTTTPERVAEAVLKAADSKK
                     IEIAVAPLVQRTMAHIGLISPHVSHRVQSGPAAHKAADSLVAGQADKR"
     regulatory      2865..3010
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     assembly_gap    3227..3236
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3237..3651
                     /locus_tag="PLJ64_07635"
     CDS             <3237..3651
                     /locus_tag="PLJ64_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93389.1"
                     /translation="KKARLIRGIAYAPDNAPAAVKRVIAAANRIRLKPYIYGGGHTLS
                     RRLQAGYDCSGSVSYALRAGGFISYPMPSGSFTNWKQPGAGKWITTYANGGHMYMIVA
                     GLSFDTSNMSSSGGNRWSTSIRSSRGFSVRHPGGF"
     gene            3811..4782
                     /locus_tag="PLJ64_07640"
     CDS             3811..4782
                     /locus_tag="PLJ64_07640"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNC93390.1"
                     /translation="MEKRKLGSEGLEVSAIGLGCMGMSEFYGEGDEAESIATIHRALE
                     VGVNFLDTADMYGPWTNEELVGKAISDRRDQVVLATKFGIRRGDDPRDRHVDGRPEYV
                     RESIDGSLKRLGVDHVDLYYQHRVDKTVPIEETVGAMAELVEAGKVRYLGLSEASAES
                     IRKANSVHPISALQTEFSLFERGASDKVIPTLRELGIGFVPYSPLGRGFLTGRFRSGA
                     DFGSGDFREHDPRMSQENMAANMAIVEAIEDLASRHDVSPAQMAIAWVLAQGDDFVPI
                     PGTKKVSRLEENAAAAEVSLSDEELASLDALPRPQGDRYANMASIDE"
     gene            4775..5965
                     /locus_tag="PLJ64_07645"
     CDS             4775..5965
                     /locus_tag="PLJ64_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNC93391.1"
                     /translation="MSENPAGAAVDAGPGIPPPFTREHEELRETVRRFVENEIAPHVD
                     EWEDARLFPRELFDRCGELGFLGLKFPEEYGGQGGSYLHDAIWVEELSRRGASGGVAA
                     GINAHASIAMPPVFKFGDEWQRRQWLVPGIRGEKIGALGITEPGAGSDVASIRTFARK
                     VDGGYVVNGSKTFITNGVRADFLVCAVKTTEEGGHNGISFLVIDTDSPGYEVVTKLEK
                     LGWHASDTGEISFTDVEVPEEHLLGDENEGFRLIMANFAWERLLMAIGAVGAMDALVD
                     ASITYANQRQAFGRNIGSFQAIRHKIAEMATTAAAAKTLTYDTLRRFDEGEPVIREVS
                     MTKLMTQRALCEVADEALQIHGGYGYMKEYGIERALRDARLGPIGGGTDEIMKDIIGK
                     TFGL"
     gene            6095..7126
                     /locus_tag="PLJ64_07650"
     CDS             6095..7126
                     /locus_tag="PLJ64_07650"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HNC93392.1"
                     /translation="MPAGAPAAAKGSADRGASASVVGGSNASAASFPWQVLITANDEE
                     FCGGVLIHPKIVLTAAHCLVDDNWNYYEDLEGVTFRAFTGRTRLNSGGEELDWKVARA
                     DPDYDPDTNAYDWGFISLNSPASAPTLKLAGPNERSLWEPGRAATVSGFGDLSDGGQG
                     ATILQKAKIPLLADSGCSRYGASFHSSTQLCAGYLRGGRDACQGDSGGPLSVTADHGE
                     RRLVGLVSTGRGCAEAGFPGIYSKVAGAVNSDRIQQEVKFIETEDDFGAAYTGINVIG
                     SGARPLGCSAAVKSGKTAAQKLKSAQRRLKAARRSGRANRIEAARRGVERARHKLGRS
                     RVKSRKACT"
     gene            7123..8643
                     /locus_tag="PLJ64_07655"
     CDS             7123..8643
                     /locus_tag="PLJ64_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93393.1"
                     /translation="MNFPIRNLMTKSLAGALALAGLAAILLAPSASAAPIANAGEAPS
                     LLDFSGKAFKATPVAGASKAPQNPFMAKNQKSNIHNDTWMTGTYTWPGPLGRNLVTNS
                     GSAANGVCSTIAFDSHGDIVTVCVSTIAAPQARIVDPDTLEVIDTYDLPNADPVGNTP
                     AYQNFSAGGYFYLDNQDRLVIPTRNNHIYVLGQTPDGSQFQLVKDYDVTSVVDPATER
                     MNSALPDFQGDIWFVVKQTGEVGTVDRKTGQVRYLRLGVGIQNSFTVDRNAVYVVTSK
                     RIYRMWKNGNGKPYVVWSAKYRNTGVQKPGQADAGTGTTPDVMSGGYVSMVDNANPEN
                     IVVYRTAAKLHGKKRKVCEVPVFGKDASATENSLITTGRSMIVENNYGYTNIFDPGAG
                     AVVTRAGFARVDIRKDGKGCKKIWTNKTERAPSVVPKMSAKTGLIYSYTRPPDPSGAQ
                     GYYWSAIDYRTGKTVWNKYAGSGFLYNNNYSGLGLGPDGTAYLGVIGGIISLKDGS"
BASE COUNT         1569 a         2835 c         2828 g         1406 t
ORIGIN      
        1 accaccaggt ccttctcgtc gatcgccgcc gagatctcgg gggctagcgg tccggagttg
       61 ccgatgcagg tggtgcagcc gtaaccgaca aggttgaact ggagctggtc caggtactgg
      121 gtcaggccgg cattgtcgag gtagtcggtg accacggtcg agccgggagc cagcgaggtc
      181 ttcacccagg gcttgcggga aagacccttg gccagggcat tcctggccag caggccggcc
      241 ccgaccatca catacgggtt cgaggtgttg gtgcaggaag tgatcgcggc gatcacgacg
      301 cgaccgtggt cgaggtcgaa ggaggtgccg tcctcgagcg tgactccgat catgtcttcg
      361 ctgcaacgct gctcggcaac cctggtgctg gctctggtcg gcgcgccggc cggcttgccg
      421 agatcactcg tgacatggtc ttcacccggg ggatcggaag cggggaagga ttcctccagc
      481 gcctcgtcat aggcgctgag ctggttcgag gcgtcctcgt cgaattcgca gagcgcctcg
      541 agaaaggcag gcttggcgtc cgagagcgag acccggtcct gcggccgctt cgggccggcg
      601 atcgatggca ccaccgagtc gagttcgagc tcgagcgtgt cggagaagac gggatccccg
      661 gaatcggcgg tgtggaaggt gccctgcagg cgggcgtagg catcgaccag ctcgatcgtc
      721 tccgtcggcc ggccggtcag ctccaggtac ttcagcgttt cggcgtcggg cgggaagatc
      781 gcgcaggtcg agccaaactc cggcgacatg ttgccgaggg tcgcccggtc ggccagcggc
      841 aggttcgaaa ggcccgggcc gtagaactcg acgaacttgc cgaccacccc gcgctcgcgg
      901 agcatctcgg tcacggtcag gaccaggtcg gtggcggtcg cggcggccgg cagcttgccg
      961 tgaagcttga agccgattac ctgcggcagc agcatcgaaa cgggctggcc gagcatcgcc
     1021 gcctcggctt cgatgccgcc gaccccccag cccagcacgc caaggccgtt gaccatggtg
     1081 gtgtgcgagt cggtgccgac cagggtgtcc ggataggcct gaaggacacc gtcgttctcg
     1141 cggctgtaga cgacctgggc gatgtattcg aggttgacct gatggcagat gccggtcgcc
     1201 ggcgggacca cgcggaagtt gttgaaggag ccctggcccc acttgaggaa ctcgtaacgc
     1261 tcgaggttcc gctcgaactc cttctcggcg ttgatctcga aggccatgcc gttgccgaag
     1321 gcatcgacct ggaccgagtg gtcgatcacc aggtcgacgt caaccagcgg gttgatcgcg
     1381 gaaacctcgc cgccgatgtc ggccatcgcg tcacgcatcg cggccaggtc gaccacggcc
     1441 ggcaccccgg tgaagtcctg catcaggacc cgggcgggct ggaagggaat ctcgtccgag
     1501 ggctcggcct tcgcatccca cgaggcgatc gcctcgacgt cggcggcgct gaccgagaca
     1561 ccgtcctcaa gccgcagcac gttctcgagc agaaccttga tcgagtaggg caggcgggcg
     1621 acgtcgaact tcttctgcag ggcgtcgaga cggaagatct cgtagctcct gccgcccgtt
     1681 tcgagtgtgt ccttgcttga aaacgagtct ttggaattgc tgctcatggt tctcttctct
     1741 gtctttagtg ggtcaacgtt tgtcggcctg gccggcgacc agcgagtcgg cggccttgtg
     1801 ggcggccggg cccgactgga cccggtggga gacgtgcggg ctgatcagcc cgatgtgggc
     1861 catggtccgc tggaccaggg gtgcgacggc gatctcgatc ttcttcgagt cggcggcctt
     1921 aagcactgct tcggccaccc tttcgggggt ggtcgtgccg aggccgggcg gggcgtcggc
     1981 tccggagtcc gcgaacatgc ccgcctcgcg gacgaagccg ggagcgacca gggtgatgct
     2041 gaccggggaa ccggccaggt ccgcggcgag cccgaaggcg aagccgcgca aaccgaactt
     2101 ggtcgcgtta tagatggagg agcgggggct gcccgccttg ccggccaggg agccgatcat
     2161 caccagcttg ccctggcccc gttccagcat ccccggcaac agggcctggg cgagcaggat
     2221 cggtgattcg aggttgatcc gcagcatgcg ggcaacctgt tcgtcggtca ggtcggtgat
     2281 tcgaccggtg ctgggcagag ccgcattggc gaccagcatg tcaaccgctc cggcctgatt
     2341 ggcaagttcc tgcgcggcac caacgagtcc gaggtcggcc gccagcaccg agtggccgct
     2401 gccgggcagg ctgtcgacca gctcggtgag cgcctcggcc gaacggccgg acacgatcac
     2461 ctccgccccg gccgccgcca gggtgctcgc gatcctgcgc ccgatacctc cggtcgcacc
     2521 ggtcagaagc accttcttgc ccgcgatatc caccagtcaa acctagccga cacaaggagt
     2581 cgggcggggt ccaacccgcc ctcccagaga aacccgcccc gcccactcga agcaaagaat
     2641 ggttgggcgg gactccagcg gaagtgcctc cagggtgatc aggcctgaag cttcaccgaa
     2701 cggagccggc ccatccgttc ccgggtgtcc tgggccggcg gcttgagctt ggaggcgggg
     2761 cgaagctcgc cgactactta tgtatggaaa ttgcagggat gaacaaaggg atcggggggt
     2821 ttgttagcga atgacatgca gatcgtctat gatcccgcaa gttcataacc gagacgccga
     2881 atcccgactc actctgtgac cgggatcggg ggaaccaagt ttctggggcg aacccactga
     2941 ttcaggtggg ggcgcggctc ctaaagcgcc agcccgacag ctaaccccgt aggccgacca
     3001 aggagttcgg tatcaacctg tttcacaaaa atcagacgtc agttgctttg aatacttccc
     3061 gggcttccgc cgttgccgcc gcatttgccg caattgcctt catcgccttc ttctcctttg
     3121 ccggccacgc ccaggccgcc ggctgcaagg acgcctcgag tggcgggctc accctcgccc
     3181 cggccgactg caaggccccc accgccctgt gcgacgtggc ctcgggnnnn nnnnnncaag
     3241 aaggcccggc tgatccgggg catcgcctac gccccggaca acgctcccgc cgccgtcaag
     3301 cgggtcatcg ccgccgccaa ccggattcgc ctcaagccgt acatctacgg tggcggtcac
     3361 accctcagcc gcaggctgca ggccggctat gactgctccg gctcggtcag ctacgcgctc
     3421 cgcgccggcg gcttcatcag ctacccgatg ccttccggca gcttcaccaa ctggaagcag
     3481 cccggtgccg gcaagtggat caccacctac gccaatggcg gtcacatgta catgatcgtg
     3541 gctggcctct cgttcgatac ttcaaacatg agctcctccg gcggcaatcg ctggagcacc
     3601 tcgatccggt catccagggg attcagcgtc aggcaccccg gcggcttcta gacctgccga
     3661 cacagcagaa tcgcgaaacg ccccgcaagt ccggggcgtt tcttttgcat caacccgttc
     3721 ccttcccgcc cggcgtgcca cgacggggtc gaccggccat gattccgggg acgagggggc
     3781 cggggagcgc gccggttcta tgatcgacag atggagaaac gcaagctggg cagcgaaggg
     3841 ctcgaggttt ccgcaatcgg gctgggctgc atgggcatgt ccgagttcta cggtgaaggc
     3901 gacgaagcgg aatcgatcgc gaccatccac cgggccctgg aggtcggagt caatttcctc
     3961 gacaccgccg acatgtacgg gccctggacc aatgaggaac tggtcggcaa ggcgatctcc
     4021 gaccggcgcg atcaggtggt gctggcgacc aagttcggga tccgccgggg agacgacccc
     4081 cgcgaccggc atgtcgacgg ccggcccgag tacgtgcggg aatcgatcga cggctcgctg
     4141 aagcggctcg gggtcgatca cgtcgacctc tattaccagc accgggtcga caagaccgtg
     4201 ccgatcgagg agacggtcgg agcgatggca gagctggtcg aggcgggcaa ggtccgctat
     4261 ctgggacttt ccgaagcctc ggccgaaagc atccggaagg ccaattcggt ccatccgatc
     4321 tcggcgctcc agaccgagtt ctcgctcttc gagcggggcg cgagcgacaa ggtgatcccg
     4381 accctgaggg aactcgggat cggcttcgtt ccctactccc ccctgggccg cggctttctg
     4441 accgggcggt tccgttccgg tgccgacttc ggcagcggtg acttccgtga gcacgacccg
     4501 cggatgagcc aggagaacat ggccgcgaac atggcgatcg tggaggcgat cgaggatctc
     4561 gccagccgtc acgatgtctc ccccgcccag atggcgatcg cctgggtcct cgcccagggc
     4621 gacgatttcg tgccgatccc cggcaccaag aaggtcagcc gcctcgagga gaacgcggct
     4681 gcggcggagg tcagcctgag tgatgaggag ctggcctcgc tggatgccct gccgcggccg
     4741 cagggtgatc gctacgccaa catggcttcg atcgatgagt gaaaacccgg ccggggcggc
     4801 ggttgatgcg ggacccggca tcccgccgcc cttcacccgg gaacatgagg aactccgcga
     4861 gaccgtacgg cgtttcgtcg aaaacgagat cgccccccat gtcgacgagt gggaggacgc
     4921 ccggcttttc ccccgcgagc tcttcgaccg ctgcggcgag ctcggctttc tcggtctgaa
     4981 gttccccgag gagtacggcg gccagggcgg cagctacctg cacgacgcga tctgggtcga
     5041 ggagctttcc cggcgcggcg cttccggagg agtcgccgcc gggatcaatg cgcacgcctc
     5101 gatcgccatg cccccggtct tcaagttcgg ggatgaatgg cagcgccggc aatggctggt
     5161 ccccgggatc cgcggcgaga agatcggtgc cctcgggatc accgaacccg gcgccggttc
     5221 cgacgtggcc tcgatccgca ccttcgcgcg gaaggtggat ggcggctacg tggtcaacgg
     5281 atcgaagacc ttcatcacca acggggtccg ggccgacttt ctggtctgcg ccgtcaagac
     5341 caccgaagag ggtggccaca acggcatctc gttcctggtg atcgacaccg actcacccgg
     5401 gtacgaggtg gtgaccaagc tggagaagct cggctggcat gcctcggaca ccggcgagat
     5461 ctccttcacc gacgtggagg ttccggaaga acatctgctg ggtgacgaga acgagggctt
     5521 ccgcctgatc atggccaact tcgcctggga gcggctgttg atggcgatcg gcgccgtggg
     5581 ggcgatggac gccctggtcg atgcttcgat cacctatgcg aaccagcgcc aggccttcgg
     5641 ccggaacatc ggttccttcc aggcgatccg gcacaagatc gccgaaatgg cgaccacggc
     5701 ggccgccgca aagactctca cctacgacac gctgcgccgc tttgacgaag gcgaaccggt
     5761 gatccgtgaa gtctcgatga ccaagctgat gacccagcgg gcgctctgcg aagtggccga
     5821 cgaggccctc cagattcacg gtggctacgg ctacatgaag gaatacggga tcgaaagggc
     5881 tttgcgggat gcccggctcg gcccgatcgg cggcggtacc gacgaaatca tgaaggacat
     5941 catcggcaag acctttggcc tgtagccggg ttgttatctg tagtccccga gcgggacctc
     6001 gcacctgaac caccgaccaa gcggagaacc cctgaaaacc ggaacgaaac tggctgctct
     6061 ccttgcgctt tcgctgacgc ttgcgggact caccctgccc gcgggtgccc cggccgccgc
     6121 gaagggctcg gctgaccggg gagcctcggc gtccgtcgtc ggcggcagca acgccagcgc
     6181 ggccagcttc ccctggcagg tcctgatcac cgccaacgac gaagagttct gcggcggggt
     6241 gctgatccat ccgaagatcg tcctcaccgc cgcccactgt ctggtcgatg acaactggaa
     6301 ctactacgaa gacctggaag gggtcacctt ccgggccttc accggtcgca cccgtctgaa
     6361 ctcgggcggc gaggagctcg actggaaggt cgcccgggcc gacccggatt acgaccccga
     6421 caccaacgcc tacgactggg gcttcatctc gctgaacagc cccgcctccg ccccgaccct
     6481 gaagctggcc ggaccgaacg agcgttcact ctgggagccc ggccgggccg cgacggtctc
     6541 cggcttcggc gacctttcgg acggcggtca gggtgccacg atcctccaga aggcgaagat
     6601 cccgctgctg gccgactccg gatgttcccg gtacggggcg agtttccact cttccacgca
     6661 gctctgcgcc ggctacctgc gaggcggcag ggacgcctgc cagggcgaca gcggagggcc
     6721 gctgagcgtc accgcggatc acggcgagcg caggctggtc ggcctggtca gcaccggacg
     6781 gggttgtgcc gaggccggct tcccgggcat ctactcgaag gtcgccggag ccgtgaacag
     6841 cgaccgcatc cagcaggaag tcaagttcat cgaaaccgag gatgatttcg gcgccgccta
     6901 caccgggatc aacgtgatcg gctccggtgc caggccgctc gggtgttcgg ccgcggtcaa
     6961 atcaggaaaa acagccgcgc agaagctaaa gtcagctcag cgaaggctca aggccgctcg
     7021 caggtcgggg agggcaaacc gaatcgaagc tgcgcggcgg ggtgttgaaa gagccaggca
     7081 caagctcggc aggagccggg tgaaatcgag aaaggcatgc acttgaactt cccgatccgc
     7141 aatctgatga cgaagtcgct ggccggcgcc ctggccctgg ccggactggc cgcgatcctc
     7201 cttgcccctt ccgcatcggc cgcgccgatc gccaacgcgg gtgaagcccc ctcgctgctc
     7261 gacttttccg gcaaggcctt caaggcgacc ccggtcgccg gcgcgtcgaa ggcgccgcag
     7321 aacccgttca tggcgaagaa ccagaagagc aacatccaca acgacacctg gatgaccggc
     7381 acctacacct ggcccggccc gctcggccgg aacctggtca ccaactcggg ttccgccgcg
     7441 aacggcgtct gctcgaccat cgccttcgac agccacggcg acatcgtcac ggtctgcgtt
     7501 tccacgattg ccgcaccaca ggcccggatc gtcgacccgg acacgcttga ggtgatcgat
     7561 acctacgacc ttccgaatgc ggacccggtc ggaaacaccc cggcgtacca gaacttttcg
     7621 gccggcggct acttctatct cgacaaccag gaccggctgg tcattccgac ccgcaacaat
     7681 cacatctacg tgctcggcca gacccccgac ggaagccagt tccagctcgt aaaggactac
     7741 gacgtgacct cggtcgtcga ccccgcaacc gaacgcatga actcggccct gcccgatttc
     7801 cagggtgaca tctggttcgt cgtcaagcag acgggcgagg tcggcacggt cgatcgcaag
     7861 accggccagg tcaggtacct caggctcggc gtggggatcc agaactcgtt caccgtcgac
     7921 cggaacgcgg tctatgtagt gaccagcaag cggatctacc ggatgtggaa gaacggcaac
     7981 ggcaagccct atgtggtctg gagcgcgaag taccggaaca ccggcgtcca gaagcccgga
     8041 caggccgacg ccggcaccgg aacgactccc gacgtgatga gcggcggcta cgtttcgatg
     8101 gtcgacaacg ccaatccgga gaacatcgtg gtttaccgga ccgcagcgaa actccacggc
     8161 aagaagcgga aggtctgcga ggtcccggtc ttcggcaagg acgccagcgc caccgagaac
     8221 tcgctgatca ccaccggccg ctcgatgatc gtcgagaaca actacggcta cacgaacatc
     8281 ttcgatcccg gagccggtgc cgtggtgacc agggccggct ttgcccgggt cgacattcgc
     8341 aaggacggca agggctgcaa gaagatctgg accaacaaga ccgagcgggc gccttcagtg
     8401 gttccgaaga tgtcagcgaa gaccggcctg atctactcct acacccgtcc gcctgatccg
     8461 agcggcgccc agggttacta ctggtcggcg atcgactatc ggaccggcaa gacggtctgg
     8521 aacaagtacg cgggctccgg cttcctctac aacaacaatt actcgggtct cggtctcggc
     8581 cccgacggca ccgcctacct cggagtgatc ggcggcatca tctccctcaa ggacggcagc
     8641 tagcccga
//
LOCUS       DAOQKN010000098         8634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_3919_length_8634_cov_5.927264, whole genome shotgun sequence.
ACCESSION   DAOQKN010000098 DAOQKN010000000
VERSION     DAOQKN010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 8634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8634
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3919_length_8634_cov_5.927264"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="PLJ64_07660"
     CDS             complement(<1..309)
                     /locus_tag="PLJ64_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNC93394.1"
                     /translation="MTPYYDLLNKPCGGTFDDIVDSIGHTPLVELKTLSPKEDVRIFA
                     KLESANPTGSVKDRVARSMMQKAEEEGAIAPGQTILEPTSGNTGISLAMICRRKGYPL
                     K"
     gene            445..1305
                     /locus_tag="PLJ64_07665"
     CDS             445..1305
                     /locus_tag="PLJ64_07665"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNC93395.1"
                     /translation="MKKSRLIALLSALMILAAMVVVVGCGSSDDDSSSSDNGSTAASG
                     DFSSELINEGTLTVGTDTPYPPFEFGDAPDYNGFDVNLVDEIANRLGLETKWVDTSFD
                     TIFTDVAQGKFDMVASASTITPDRQKTVNFSDPYYSAQQALLVPEGSDISSVEDLDGK
                     TVGAQNGTTGKDFAENETNASRVQGYPNGPAAIEAVKNGQVEATIIDQPVAQDAIDKG
                     QTGFEVATTIPTGELYGFAFAKDTPELLAAVNKTLAEMKDDGSLDKIYQKYFSINAPE
                     EVVNGTTTNP"
     gene            1387..2127
                     /locus_tag="PLJ64_07670"
     CDS             1387..2127
                     /locus_tag="PLJ64_07670"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HNC93396.1"
                     /translation="MGEFFQTYFDFSIMGDNFNAVLEGFWWTIKLSLIGGAFALAWGL
                     ILSVIRQLPGKALAPVRWLAIAYIDCLRGIPMLLTLLLIYGGLGTLQSTGTIPEWLGI
                     PEFFGWPSSFWYGVMALTLTYGAYMAEVYRAGIESIPRGQMEAARSLGMSHGQAMRFV
                     IVPQAIRRVIPPLLNDYIALMKDTSLVSVISLAEVVHVGQDVYSTTFNPSALTLGAIM
                     FLIVTLPLARLVDWQIKRQDEKFQRGLA"
     gene            2175..2909
                     /locus_tag="PLJ64_07675"
     CDS             2175..2909
                     /locus_tag="PLJ64_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002183294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HNC93397.1"
                     /translation="MLKVSNLKKSYGKLEVLRDIDLDIKKGEVICVLGPSGSGKSTML
                     RCLNLLESPTGGTIELKGDIVFDGTKPKNLDDLRRETGMVFQSFNLFPNMSAKENVSI
                     AQVRVRGRKKADADKRSIEQLTKVGLAEKVDEYPDRLSGGQQQRVAIARALAMDPEVM
                     LFDEVTSALDPELVKGVLDVMRELADSGMTMICVTHEIGFAKEVADRVIFMDGGYIVE
                     QGPPKDVLDAPREERTKKFLGLVLDH"
     gene            2909..>3408
                     /locus_tag="PLJ64_07680"
     CDS             2909..>3408
                     /locus_tag="PLJ64_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93398.1"
                     /translation="MEAGGIETPGEKTVVLSGRAYEFGHMDTMISGASIRVREFPELA
                     AVTDESGDFRLEVPDQALVTPYIESGSGTNIRRPREGEPFEVETHWNEIDLQTFQTDG
                     RDLFNVNFQTPPDFEFEALKALLQVPSGEDGRPLKSVIVTTACARNVRGVDYDTFWLR
                     TPHGVAG"
     assembly_gap    3409..3423
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3424..3731
                     /locus_tag="PLJ64_07685"
     CDS             <3424..3731
                     /locus_tag="PLJ64_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93399.1"
                     /translation="AEPALPDPVYFNEQVIPDSSLTETSIDGGIIWPVVETGTYTITT
                     TSPTAGFASFTATCAPGRVVNANPPWGAFELVGAGEEPDGGPEFPAGCRVGAVPSAT"
     gene            3815..4963
                     /locus_tag="PLJ64_07690"
     CDS             3815..4963
                     /locus_tag="PLJ64_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93400.1"
                     /translation="MRRTVGVLAAFGLAVMMLFGAQSASAAGKTVTISGKAFIFNYMD
                     TPIPGATIKVREFPKLSAVTNDQGDYELTVPDDANVTPYIEPGGPVNLTRYNKDGSLK
                     GILDNTHWNTIDLQTFHTRGQDLENANFQTPRDAEYNGLKALLAIPSSPDGRPAQCAI
                     VTTSSARNVRGVDYLTYWNNTPHGVAGATSIEYPALDGPVYFNDSVIPDATKTESSGD
                     GGIIWPIVPTGTYRIVTTSPDTQFASFLATCKPGRVINANPPWGAYELSPGEKPLGAS
                     NVAAKLVSAKAVRKSKTRRQLSLVLNSGENIDATVTLAAGGMTALRTLPLKPGNKTLT
                     FNLGAKVKAKQAKVTVKLTDASGVSFTTVKKVNVPKIIKKKNGKGKRK"
     gene            complement(4976..5785)
                     /locus_tag="PLJ64_07695"
     CDS             complement(4976..5785)
                     /locus_tag="PLJ64_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93401.1"
                     /translation="MTWIETTSLTFTARHEEGDRSYAEELLDRLEDLSLKLEDRFEVV
                     PGDISIVIHPSPVWLTAAHPFLPFVRYAAAPAGRRYLAGWPMATEVHVLGEKAMEKRA
                     AGEASAEALRGTAERLYTQIVLAANNERIPPPWTPMRFLRYLNWAWLVEGSAQHFARQ
                     VDFFRPAVIRRLREGSRPSFPPSRRDAIILGGTIFDLLEDYRGGGACELLIGRLRRDG
                     PVANLEVAFDLRIREVEEMWREYLRDLARPQPDSTSLPSPEELLGPIQPEP"
     gene            complement(5908..6540)
                     /locus_tag="PLJ64_07700"
     CDS             complement(5908..6540)
                     /locus_tag="PLJ64_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1802 family protein"
                     /protein_id="HNC93402.1"
                     /translation="MPIAFKEWAVTVRALAEGEQLLTLRKGGIREENKHFELEHDRFF
                     LYPTFDHQHNDLVRASHHPELDRALEEGVWPDEEPPARALLQDGGIPQPDRVRIRAWA
                     EVAEDITIEDPRVVDALSPFYIWTTDYAHKRLSWKPRHPLHLVLLRVHRIPRPVTVRV
                     REEYHGCRSWVEIDRDLPFEGTPVMSDEEFEFATNEIRSICAGDREPALV"
     gene            complement(6720..>8634)
                     /locus_tag="PLJ64_07705"
     CDS             complement(6720..>8634)
                     /locus_tag="PLJ64_07705"
                     /inference="COORDINATES: protein motif:HMM:NF014071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HNC93403.1"
                     /translation="DDLERMARDLHEAEETRDQAILGVDVGGTFTDAVLITGDRIFTG
                     KSPSTPGDQSLGVIEATRQVLDKAGIKADRIGHFAHGMTVATNALLESKGARTAFVAT
                     RGFTDLIEIGRQDRASLYRLQAAHPAPLVPPERRVGVAERNGPEGALEELTEAEAGRV
                     AEAVAGLDVESVAVCLLHSYRFPENEQRIGEAIRQRCGEDLHVSLSHDVVGTFREFER
                     AATTEIDAALSPLLAGYLERLARRSDEEGLPRPEIMQSSGGVAGLEEVADHATVAILS
                     GPAGGAAAASLIAEQSGEKNLLCFDMGGTSCDVCVVEGGRVHEAASKKIAGRPIALPM
                     VDIDTVGAGGGSIAWRDSGGALRVGPESAGARPGPACYGHGGTRPTVTDANLVLGRLN
                     AGEELAGGIVLDLDLARQAIETLAGQLGIPTDRCAEGIIRVANAEMVRALRVVTVQRG
                     LDPRRFTLLAFGGAGPLHAAGVAEELGISTVLVPPTGGVFSALGLAAADRRRDEVQTV
                     LAAEESLDRQRLLDLIADADETGWDLRYRGQSFELTVSDDSADPARLRELFEAAHEER
                     YGYRDPASPIELVTVRRRWVVPGPGFAPAPPSETVLSGPATIDLGEATLHLPVGWNAE
                     GEGGGLLRMFREL"
BASE COUNT         1555 a         2802 c         2832 g         1430 t
ORIGIN      
        1 cttgagcggg tagcccttgc ggcggcagat catcgccagc gagatgccgg tgttgccgga
       61 ggtcggttcc agaatggtct ggccgggggc gatcgcgccc tcctcctcgg ccttctgcat
      121 catcgaccgg gcgacccggt ccttgaccga gccggtcgga ttggcggact cgagcttggc
      181 gaagatccgc acgtcttcct tcggcgaaag cgtcttcagt tcgaccagcg gggtgtgacc
      241 gatcgagtcg acgatgtcgt cgaaggtgcc cccgcagggc ttgttgagaa ggtcgtagta
      301 aggagtcatc ttgtgggccc gctggacggg ggtacttcat aaagtgtagt cacttggacg
      361 ccggtcggaa gatcttcccg gaaatcgtga aatactggcc gggttgcgcg tccagcaaac
      421 aattccctcc tggaggaacc tggcatgaag aaatcccgat tgatcgcgct gctttcggcg
      481 ctgatgatcc tcgcggcgat ggttgtcgtg gttggctgcg gtagcagcga cgatgacagc
      541 tcgagcagtg acaatggcag caccgctgcc agtggcgact tttcgtccga actgatcaac
      601 gaaggcacgc tgactgtcgg aaccgacacc ccgtacccgc ccttcgagtt cggcgatgcg
      661 cctgactaca acggcttcga cgtgaatctc gttgacgaga tcgccaatcg tctcggcctc
      721 gaaaccaagt gggtcgacac ctcgttcgac acgatcttca ccgatgttgc ccagggcaag
      781 ttcgacatgg ttgcttcggc ttccacgatc acgcccgacc ggcagaagac ggtgaacttc
      841 tcggacccgt actactcggc ccagcaggcg ctgctcgtgc ccgagggttc ggacatcagt
      901 tcggttgagg atctcgacgg caagactgtc ggcgcccaga acggcaccac cggcaaggac
      961 ttcgccgaga acgagaccaa cgccagccgt gttcagggtt acccgaacgg tccggccgcg
     1021 atcgaagccg tgaagaacgg ccaggtcgaa gcgaccatca tcgaccagcc ggttgcgcag
     1081 gacgcgatcg acaagggtca gaccgggttc gaggtggcaa cgaccatccc gaccggtgag
     1141 ctctacggct tcgccttcgc gaaggacacg ccggagctgc tggcagcggt caacaagacg
     1201 ctcgcggaga tgaaggatga tggctctctg gacaagatct accagaagta cttcagcatc
     1261 aacgcgcccg aagaagtagt gaacggaacc acgaccaacc cgtagttccg aagggaatcg
     1321 acagaagggg cgtccccgag acgccccttc tgtccggttc tcgaacaagt gacgggggag
     1381 tatcagatgg gtgagttttt tcagacatat ttcgacttct ccatcatggg ggacaacttc
     1441 aacgctgtcc tcgaaggttt ctggtggacg atcaagcttt ccctgatcgg cggagccttc
     1501 gccctcgcgt ggggcctgat cctctccgtc attcgccagc tgccgggcaa ggctctggcc
     1561 ccggtcaggt ggctcgcgat cgcttacatc gactgcctcc gcggcatccc gatgctgctt
     1621 acgctgcttt tgatttacgg cggtctcggc accctgcaat caaccggcac gatcccggag
     1681 tggctgggaa tccccgagtt cttcggctgg cccagctcgt tctggtacgg cgtcatggcg
     1741 ctgaccctca cctacggcgc ctacatggcg gaggtctacc gcgccggcat cgaatcgatc
     1801 ccgcgcggcc agatggaagc cgcccgctcg ctgggtatga gtcacggcca ggcgatgcgg
     1861 ttcgtgatcg ttccccaggc gatccgccgg gtgatcccgc cgctcctcaa cgattacatc
     1921 gccctgatga aggacacctc gctggtcagc gtgatcagcc tcgccgaggt cgttcacgtc
     1981 gggcaggatg tctattcgac gactttcaat ccctccgccc tcaccctggg cgcgatcatg
     2041 ttcctgatcg tcaccctgcc gctggcccgc ctggttgact ggcagatcaa gcgccaggac
     2101 gagaagttcc agcggggtct cgcatgagcg acgaaacggt gtccctgagc gaggatgtag
     2161 cccgcgggga aacgatcctc aaggtcagca acctgaagaa gagctacggc aagctcgagg
     2221 tgctgcggga catcgacctc gacatcaaga agggcgaggt catctgcgtg ctcggtccga
     2281 gcggctccgg caagagcacc atgctgcgtt gcctgaacct gctcgaatca ccgaccggcg
     2341 gcacgatcga gctgaagggc gacatcgtct tcgacgggac caaaccgaag aacctcgatg
     2401 acctgcgacg cgagaccggc atggtcttcc agtccttcaa cctcttcccg aacatgtcgg
     2461 cgaaggagaa cgtctcgatc gcccaggtcc gggtgcgcgg gcgcaagaag gccgacgccg
     2521 acaagcgctc gatcgagcag ctgaccaagg tcggcctggc cgaaaaggtt gacgagtatc
     2581 ccgaccggct ttccggcggc cagcagcagc gggtcgcgat cgcgcgggcc ctcgccatgg
     2641 acccggaagt gatgctcttt gacgaggtaa ccagcgcgct cgaccccgag ctggtcaagg
     2701 gcgtgctcga cgtgatgcgc gagctggccg actccggcat gacgatgatc tgcgtgaccc
     2761 atgagatcgg tttcgccaag gaggtggccg accgggtgat cttcatggac ggcggctaca
     2821 tcgtcgagca gggtccgccg aaggacgtgc tcgacgcccc cagggaagag cggaccaaga
     2881 agttcctcgg gctggtgctc gaccactgat ggaggcgggc ggaatcgaaa cgcccgggga
     2941 aaagacggtc gtcctgagcg gccgggccta cgagttcggt cacatggaca cgatgatctc
     3001 gggggcgagc atccgggtcc gcgagttccc ggaactggcc gccgtgaccg acgagtccgg
     3061 cgacttccgg ctcgaggtgc cggaccaggc cctggtgacc ccatacatcg aatccggcag
     3121 cggcaccaac atccggcgcc cccgggaggg cgagcccttc gaagtcgaga cccactggaa
     3181 cgagatcgac ctgcagacct tccagaccga cggccgggac ctgttcaacg tgaacttcca
     3241 gaccccaccc gacttcgagt tcgaggcgct gaaggcattg cttcaggttc cctccgggga
     3301 ggacggccgg ccgctcaagt cggtgatcgt gaccaccgcc tgcgcccgca acgtgcgcgg
     3361 cgtcgattac gacaccttct ggctgagaac gccccacggt gtggcgggnn nnnnnnnnnn
     3421 nnnccgccga gccggcccta ccggatccgg tctatttcaa cgaacaggtg atccccgatt
     3481 caagcctcac cgagacctca atcgacggcg gaatcatctg gccggtggtc gaaaccggca
     3541 cctacacgat caccaccacc tccccgaccg ccggcttcgc cagcttcacg gcaacctgcg
     3601 cccccggccg ggtcgtcaac gcgaacccgc cctggggtgc cttcgagctg gttggggccg
     3661 gtgaggaacc ggacggcggg cctgaattcc cggctgggtg ccgggtggga gcggtcccgt
     3721 ccgcaacttg atgtggtcca acctgttgta gattccggaa ccacactcat taccgtccgg
     3781 gacgacccgg gcaggagaaa gcaggagatt gatgttgagg agaactgttg gcgtgctggc
     3841 cgctttcggg ctggccgtga tgatgctgtt tggcgcccag tcggcgtcgg cggcgggcaa
     3901 gaccgtgacg atcagcggca aggcttttat tttcaactac atggacacgc cgatcccggg
     3961 ggccacgatc aaggtccgcg agtttcccaa gctttctgcg gtcaccaacg atcagggtga
     4021 ctacgaactg acggttcccg acgacgccaa cgtcactccg tacatcgagc ccggcggccc
     4081 ggtgaatctg acccgctaca acaaggacgg ctcgctcaag ggcatcctcg acaacaccca
     4141 ctggaacacg attgacctcc agaccttcca cacccgcggc caggatctcg agaacgcgaa
     4201 cttccagacg cctcgcgacg ccgaatacaa cggcctcaag gcgctgcttg cgattccttc
     4261 ttcccctgat ggccgcccgg cccagtgcgc gatcgtcacc acctcgtctg cccgaaacgt
     4321 gcggggcgtc gactacctga cctactggaa caacactccg catggagtgg ccggtgccac
     4381 ctcgatcgag tacccggccc tcgacggccc tgtctacttc aacgactcgg tcatccccga
     4441 cgcgaccaag accgagtctt ccggagacgg cggcatcatc tggccgattg tcccgaccgg
     4501 cacctaccgg atcgtcacca cctcgcccga cacccagttc gccagcttcc tggcgacctg
     4561 caagccgggc cgcgtgatca acgccaaccc gccgtggggc gcctacgagc tcagccccgg
     4621 cgagaagccg ctcggcgcca gcaacgtggc cgccaagctc gtttcggcca aggctgtccg
     4681 caagagcaag accaggcgtc agctctccct ggtcttgaat tccggtgaga acatcgacgc
     4741 caccgtgacg cttgcggccg gcggcatgac cgcactgcgg acgctgcccc tgaagccggg
     4801 caacaagacg ctgaccttca acctcggcgc aaaggtcaag gcgaagcagg cgaaggtcac
     4861 cgtcaagctg accgacgctt ccggcgtctc gttcaccacc gtcaagaagg tgaacgttcc
     4921 gaagatcatc aagaagaaga acggaaaggg caagcgcaag tagcgctcgc cccggtcagg
     4981 gctccggctg gatcggaccg agcagctcct ccggcgaggg cagcgaggtc gaatccggct
     5041 ggggcctggc caggtccctg aggtattcgc gccacatctc ctcgacctcc ctgatccgga
     5101 ggtcgaaggc aacctcgagg ttggcgaccg ggccgtcgcg gcgcaggcgg ccgatcagaa
     5161 gctcacaggc gccgccgccg cggtagtcct cgagcaggtc gaagatcgtg cccccgagga
     5221 tgatcgcgtc acggcgggag ggcgggaaac tgggacggga gccctcgcgc aaccggcgga
     5281 tcaccgcggg gcggaagaag tcaacctgac gggcgaaatg ctgggcgctg ccctcgacca
     5341 gccaggccca gttcaggtag cggaggaagc gcatcggggt ccagggcggc gggatccgct
     5401 cgttgttggc ggccagcacg atctgggtgt agagccgctc ggcggtgccc cgcagggcct
     5461 cggccgaggc ctcgccggcc gcccgttttt ccatcgcctt ctcgcccagc acgtggacct
     5521 cggtcgccat cggccagccg gccaggtagc ggcggccggc cggggcggcg gcgtaacgga
     5581 cgaagggcag gaagggatgg gcggcggtca gccagaccgg cgaagggtgg atgacgatcg
     5641 agatgtcgcc cggcaccacc tcgaagcggt cttcgagctt gaggctcagg tcctccagcc
     5701 ggtcgagcag ctcctcggcg taggagcggt ccccctcctc gtgccgggcg gtgaaggtga
     5761 gggatgtggt ctcgatccac gtcactggcc cagcctattg caatgaaaaa gacccgccgg
     5821 agcgggcctt cttcatgaat ctcgaaaatc gagaaggcta aagggagcgc tatatggcca
     5881 ctattgcctt ctcgattgcc gggttgccta gaccaaggcg ggttcgcggt cgccggcgca
     5941 gatcgaacgg atctcgttgg tggcgaactc gaactcctcg tccgacatga ccggggtgcc
     6001 ctcgaagggg agatcacggt cgatctcgac ccaggagcgg cagccgtggt actcctcacg
     6061 gacccggacg gtgaccggac gcgggatgcg gtgaacccgc agcagaacca ggtgcagcgg
     6121 atggcgcggc ttccagctca ggcgcttgtg ggcgtagtcg gtggtccaga tgtagaacgg
     6181 ggacagcgcg tcgacgaccc gcgggtcctc gatcgtgatg tcttcggcta cttccgccca
     6241 ggcacggatg cggacgcggt cgggctgagg gatcccgccg tcctggagaa gcgcacgggc
     6301 cggcggctcc tcgtcgggcc agacgccctc ctcgagggca cggtccagct ccgggtgatg
     6361 ggaggcgcgt accaggtcgt tgtgctggtg atcgaatgtg gggtagagga agaagcggtc
     6421 gtgctcgagc tcgaagtgct tgttctcttc gcggattccg cccttgcgga gcgtgagaag
     6481 ctgttcacct tcggcgaggg ctcgcacggt aactgcccat tccttgaacg caattggcat
     6541 aggtattcag tcgggggtag aggttgctac ggataagtgg tacagcagac acctgtacgg
     6601 ggcgagagga ggcatgactg gctgccgcgg gccccgtaag gagttggcac tctacaggtc
     6661 gatccggcga aattcaagtc ctcgtgccaa aaattacgta ctaacccgta ttcacgtact
     6721 caaagttcac gaaacatgcg gagcaggcct ccgccttcgc cctcggcatt ccagccgacc
     6781 ggcaggtgca gagtcgcttc accaaggtcg atcgttgccg gtccggagag gaccgtttcg
     6841 gagggcgggg ccggagcgaa accgggaccg ggcacgaccc agcgccggcg gaccgtgacc
     6901 agctcgatcg gcgatgccgg gtcccggtac ccgtagcgct cctcgtgggc cgcctcgaag
     6961 agttcgcgga ggcgggccgg gtcggccgag tcgtcggaga cggtgagttc gaaggactgt
     7021 ccccggtagc ggagatccca gccggtctcg tcggcgtcag cgatcagatc gagcaggcgc
     7081 tggcggtcga gcgactcctc ggcggcgagc acggtctgaa cttcatcccg gcgacgatcg
     7141 gcggcggcca ggccgagggc gctgaagacg cccccggttg gaggcaccag gacagttgaa
     7201 atgcccagct cttcggcgac cccggccgca tgaagcggcc cggcgccacc gaaggccagc
     7261 agggtgaacc gccggggatc aagcccgcgc tgaacggtca cgacgcgaag ggcgcgaacc
     7321 atttccgcgt tggcgacccg gatgatcccc tcggcgcacc ggtcggtagg aatcccgagc
     7381 tgaccggcca gggtttcgat cgcctgccgg gccaggtcaa ggtcgaggac gatgccgccg
     7441 gccagctctt cgccggcgtt cagccggccg agaacgagat ttgcgtcggt cacggtcggc
     7501 cgggtgccgc cgtgcccgta acaggccggt cccggacggg ctccggcgga ttcggggccg
     7561 acccggagtg ctccgccgct gtcgcgccag gcgatcgaac cgccaccggc gccgaccgtg
     7621 tcgatatcga ccatgggcag ggcgattggc cggccggcga tcttcttcga ggctgcctcg
     7681 tggaccctgc ctccctcgac cacgcagaca tcacaggagg tgccgcccat gtcgaaacag
     7741 agcaggttct tctcgccgga ctgttcggcg atcagcgacg cggccgccgc ccctccggcc
     7801 ggtcccgaaa ggatcgccac cgtcgcgtgg tcagcgacct cctccagccc ggcgacaccg
     7861 ccgcttgact gcatgatctc cggtctcggc aggccctctt catcggagcg gcgggcgagg
     7921 cgttcgagat agcccgccag caggggggag agggcggcgt cgatttcagt ggtcgcagcc
     7981 cgttcgaatt cccggaaggt gccgaccacg tcatgcgaga gcgagacatg gagatcctcc
     8041 ccgcagcgtt gccggatcgc ctcgccgatc cgctgctcgt tctccgggaa gcggtacgag
     8101 tgaagcaggc agaccgcgac cgattcgacg tccagcccgg caaccgcttc ggccacccgg
     8161 cctgcttccg cttcggtcag ctcctcgagc gctccctcgg gcccgttccg ctcggccacc
     8221 ccgacccggc gctcgggcgg gaccagcggg gcagggtgag cggcctggag ccggtagagc
     8281 gaggcgcggt cctggcggcc gatctcgatc aggtcggtga acccgcgggt cgcgacgaag
     8341 gccgtcctgg cgcccttcga ttcgagcagg gcgttggtcg ccacggtcat tccgtgggcg
     8401 aagtgcccga tccggtccgc cttgatgccc gccttgtcaa ggacctgcct ggttgcctcg
     8461 atcaccccga gcgactggtc cccgggcgtc gagggtgact tgccggtgaa gattcgatcg
     8521 cccgtgatca ggacggcgtc ggtgaaggtg ccgccgacgt cgaccccgag gatcgcctgg
     8581 tcgcgggttt cctcggcctc gtgcaggtcg cgcgccatcc tctccaggtc gtcc
//
LOCUS       DAOQKN010000099         8554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_3968_length_8554_cov_6.485940, whole genome shotgun sequence.
ACCESSION   DAOQKN010000099 DAOQKN010000000
VERSION     DAOQKN010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 8554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8554
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3968_length_8554_cov_6.485940"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..596)
                     /locus_tag="PLJ64_07710"
     CDS             complement(117..596)
                     /locus_tag="PLJ64_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93404.1"
                     /translation="MSAAYTAAGLAVIALNLLAAVVGAVSWQRNVPSVAFWYLLRAAQ
                     IATGFFVLFACVVYATGYRASDELHYLYVFLPVAVSFLAELMRGASASQELGDRLSPR
                     EPKTSQELGELFAGLDPDRQQTIGLAIIRRETAVMTLASFVIAFLIWRALDTTAGMF"
     gene            749..1129
                     /locus_tag="PLJ64_07715"
     CDS             749..1129
                     /locus_tag="PLJ64_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNC93405.1"
                     /translation="MLHQYLPVLVFGALGIIVGGAFATLNLLLGPKKVKGDESSAAVR
                     QLDPYECGLPSEISKTFRFGVSFYLVAMLFILFDIEVVFLYPVGAILKSTDSVFVLAE
                     VILFVALLFVALIYVWRKGALDWR"
     gene            1216..1830
                     /locus_tag="PLJ64_07720"
     CDS             1216..1830
                     /locus_tag="PLJ64_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HNC93406.1"
                     /translation="MLRGDLTGEDLDKYVEERVVTTTLEKVLNQARAHSVFPATFGLA
                     CCAIEMMSTVSPRYDIARFGAEAFRASPRQADLLILSGRVSIKMAPVVRRIYDQMLEP
                     KWAISMGACSSSGGMFQNYAVVQGADKFMPVDVHVPGCPPRPEALLYGFTKLQQKIMG
                     NPDPGWRSRYNAHGTEEWARGGPASRPSEEALEAYAKAREVLGA"
     gene            1823..2482
                     /locus_tag="PLJ64_07725"
     CDS             1823..2482
                     /locus_tag="PLJ64_07725"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNC93407.1"
                     /translation="MPDAPGLELILAEINREHPGAVLDTSYDREQAALIIDPSKVIEV
                     LTWLKQTPGQEYTFLSSVHGADHFPLTPRFAVHYELLNRERYERLRVKAVIPDPGAAP
                     TEVSVVAPGSSDAGAVDSDSGERGSSMEMPETEGRSTVLDGETLPVVPSVVELFPTAD
                     FQEREVHDFFGIVFEGHPDLRRILMPEDYVGWPQRRDFPVGGEPVIFTRDEVTNPGWW
                     Q"
     gene            2482..3804
                     /locus_tag="PLJ64_07730"
     CDS             2482..3804
                     /locus_tag="PLJ64_07730"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HNC93408.1"
                     /translation="MARVEDTLHDREIRQAGAIGEAGTLRDEEITVSDFTTELAERIP
                     GVEFELEPEQEMLTVNMGPHHPATHGVLRLVVDLEGEVVKDLKPVIGYVHTGIEKSCE
                     TMQYWKVIPFVERMDYLSYFFNMQAYVGAVEKLIGLEVPRRAQWLRVLHMELNRIHSH
                     LVWLGTSALDLGAMGVYFYCFRDRDRVLDTFEMSTGQRMHTRYFQIGGVAEDIPAGFE
                     QKVRKTIEQLKTGIGQYEALLDKNEIFLIRTKDVGVVSRERLVELGVTGPLLRAAGDP
                     WDLRKEGGYLAYPELEFDVPVGTVGDGYDRYRVRLTEMRESIKIIEQILDGLPEGPWI
                     ADDRKYALPPRAELSTSMESLIHHFKLVTEGFRVEPGEVYNPIESPRGELGCFVMADG
                     SSKPARVHFREPSFVNLQALADMTIGEYVADLIQTLAMLDPILGGIDR"
     gene            3801..4556
                     /locus_tag="PLJ64_07735"
     CDS             3801..4556
                     /locus_tag="PLJ64_07735"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNC93409.1"
                     /translation="MTPVMPSDFDPRDAEPEDFVSPGLPVDRANTTGRLPVGDPEQIP
                     DPAEVEVPEDLREQIQAIVDKYPDRDHKGSPKSAAIPVLWIIQRRYGWCTPEGIVQAA
                     CVMQVTPAYLESVASFYDLLRTSPQGSHQVLVCTNLACWMRGGDELLDSFCEKTGADR
                     EAAGHGGSLSEDGEILVSGFECLGACDLAPMASVDERYFGPLENEDAATIVDQLESGE
                     EVLPEKALDKRGLAGGVADSTDPRVTGGKGPNQ"
     gene            4553..5962
                     /gene="nuoF"
                     /locus_tag="PLJ64_07740"
     CDS             4553..5962
                     /gene="nuoF"
                     /locus_tag="PLJ64_07740"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNC93410.1"
                     /translation="MSVAETRVLLRHAEDPKMATLEGYESYGGYATLKKALNEIDPEW
                     IVTELEESGLRGRGGAGFSMGKKVSFLPRTEDAKYLCCNADESEPGAFKDRELMQRNP
                     HQLIEGIIISSFGANIGHAFIFIRGEYDDQADILDRAVDEAYEAGYLGTNILGSGFNL
                     ELVVHRGAGAYICGEETALLDALEGKRGNPRLKPPFPAVQGLYGGPTLINNVETLSNV
                     SHIVANGADWFKSFGTEQSPGTKVVSVSGCVKRPGNYEVELGVTLRDLIYGLAGGPLD
                     GHEITAFYPGGSSSPVLTPAELDLPYSFEAMAEAGSMLGSGSIIVADDSVSIPRMALR
                     TAHFYRHESCGKCTPCREGTNWTEKMLERVCAGEATPMDLDIIESVQHNIMGHCLCVL
                     GDAMAMPVSSMVNKFRDEFEETIAFEAARAWDGSLDSSASPGRDVPSGDEPGPGGESP
                     EKIRRERLAGRAIPDGSGR"
     gene            5962..>8554
                     /gene="nuoG"
                     /locus_tag="PLJ64_07745"
     CDS             5962..>8554
                     /gene="nuoG"
                     /locus_tag="PLJ64_07745"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HNC93411.1"
                     /translation="MPAPKKNLITLTVDGREIEAIEGTMLVDAAKHGDIEIPVFCYEP
                     KLGAPVGACRMCLVEIEGIPKLQTACSTPVRDGMVVHTRTDQVKEAQNAVVEFLLVNH
                     PLDCPVCDKGGECPLQDISMGWGPDRSRVVDTKRHFEKPVPLSPLIAIDRERCILCYR
                     CARFSQEVSEDGQLQLLDRGAESYIGTFDDRPYIAPFQGNITELCPVGALTSYTYRFQ
                     ARPWDIEQGGSVCTLCPSQCNVSFTIRDEQVKRVLDRENDQIDDGWLCDRGRYGFQAM
                     YSQDRVMNPLRYIDGHPAPARWSDALEAASIGLAAAEGKVAALVGDASNEEAFILQDL
                     IRNGLGSADIDSRPAPTVGRDQRIRLDDPATQARVVDIDHADVVLVLGNDPIHSAPAF
                     DLRVRKAVRRNGTALAVATERPSALDGGASEVERIKPGDAAGYLANLLDQVKSGSTDG
                     IAGLLKDSGHTVVIWGERIGRGENGPVAIESLLELAGVLDLGGQEESGLIEIPDVANA
                     RGLREVGCLPDAGPGLSETEVGRDVEEIKAGLISGEVRAVILYGVDPLRDFPDTEGWR
                     QALESADFVIHFTMASTDTTEFADVVFPLESHAEKDGTITHTEGRLQRVRPAAARPGD
                     IRGGWHVLAELSAALGHETNIHAVPEALRAITEVVPFYAGLTDAEIGGRGIRWQDRSE
                     SNGWAESVGSTPDRTSPGVSGVPPTDSTTETEAVVVGGSDGTPGEVRPAEPPTSAAPT
                     DGLTLGTYRDLWANSTTDLSPALSYLIPAQRLEVSVADAERLGLSKGDEVSVKSGDTE
                     VTAFVAIRAALPEGTCFLMEGTQQGNANVFATGKPAVVEIGKASPKLEVVAGNGNGLD
                     NLVTGNGA"
BASE COUNT         1504 a         2872 c         2730 g         1448 t
ORIGIN      
        1 cctgcctcgt gtcaggacgc cggcaccagg tgagtgtggc cgcggcgtgg gtgccgacac
       61 gaggcgagtg cctccactgg cgtctgcctc gtttcaggat gcttcgaggg aggggtttag
      121 aacattccgg cggtggtatc cagggcgcgc cagatgagga aggcgatcac gaaactggcg
      181 agagtcatca ctgcggtttc gcggcggatg atcgccaggc cgatcgtctg ctggcggtcc
      241 gggtcgagtc cggcgaagag ctcgccgagc tcctggctgg tcttcggttc ccgcgggctg
      301 agccggtccc caagttcctg gctggcggat gcgccacgca tcagctcggc caggaacgag
      361 accgcgaccg gcaggaacac gtagaggtag tggagctcgt cgcttgcccg gtagcccgtc
      421 gcgtagacga cgcaggcgaa gaggacgaag aagccggtgg cgatctgggc ggcccgcagc
      481 aggtaccaga aggcgaccga tggaacgttc ctttgccagg agacagcccc gacaaccgcg
      541 gcgagcaggt tgagcgcaat cacggcgaga cccgcagcgg tataggcggc agacactgct
      601 aatcccgccc catatggtcg ggattccgct tttttatgcg aaaaatcact atgtaacgac
      661 ttgatacaaa gtcggggcgc ggaggtgtat aacccataga cagcggacac cttagttccc
      721 tgacgaaccg accacccacc tcccaccaat gctccatcag tacctcccag tcctcgtctt
      781 cggcgccctc ggcatcatcg tcggtggtgc tttcgcgacc ctgaacctgc tgctggggcc
      841 gaagaaggtg aagggggacg agtccagcgc tgcggtccgc cagctggatc cctacgagtg
      901 cggcctgcct tcggagatct cgaagacctt ccgcttcggg gtcagcttct acctggtggc
      961 gatgctcttc atcctgttcg acatcgaagt cgtcttcctc tacccggtgg gggcgatcct
     1021 caagtcgacg gactcggtct tcgtcctggc cgaagtgatc ctctttgtgg ccctgctctt
     1081 cgtggcactg atctacgtct ggcggaaggg agccctggat tggagataga acgccagaag
     1141 cttcgggttc acgcccccct cgagcagccc aagtcaccgg aagagctccg cgcccgccag
     1201 ctcaaggccc gcgacatgct gcgcggcgac ctgaccggcg aagacctcga caagtacgtc
     1261 gaggagagag tcgtcacgac caccctcgag aaggtcctca accaggcccg tgcccattcg
     1321 gtcttccccg cgaccttcgg tctcgcctgc tgcgcgatcg agatgatgtc gaccgtttca
     1381 ccgcgctatg acatcgcccg tttcggtgcc gaagccttcc gggcctcgcc ccgccaggct
     1441 gacctgttga tcctttccgg ccgggtctcg atcaagatgg cgccggtcgt ccgccgcatc
     1501 tacgaccaga tgctggaacc gaagtgggcg atctcgatgg gcgcctgctc ctcctccggc
     1561 ggaatgttcc agaattacgc ggtggttcag ggagccgaca aattcatgcc ggtcgacgtc
     1621 cacgtgcccg gctgcccgcc ccggcccgag gcactgcttt acgggttcac caagcttcag
     1681 cagaagatca tgggcaatcc cgatccgggc tggcgcagtc gctacaacgc ccacggaacc
     1741 gaggagtggg cccgcggcgg cccggcttca cgaccttcgg aggaagcact cgaggcctac
     1801 gcgaaggccc gggaggtcct cggtgcctga cgcgcccgga ctggaactca tcctcgccga
     1861 gatcaaccgc gaacatcccg gggccgtgct cgacacgagc tacgaccggg agcaggcggc
     1921 gttgatcatc gacccctcga aggtgatcga ggtcctgacc tggctcaagc agacgccggg
     1981 ccaggagtac accttcctca gctccgtcca cggcgccgac catttccccc tcaccccgcg
     2041 cttcgcggtc cactacgaac tcctcaaccg cgaacgctac gaacgcctcc gcgtgaaggc
     2101 cgtcatcccg gacccggggg ccgcgcccac tgaggtctcg gttgtcgcgc ccgggagcag
     2161 cgacgcgggg gcggtcgatt ccgattccgg tgaacggggc tcatcgatgg agatgccaga
     2221 aacggaaggt cgctcgacgg tcctcgatgg agagactctc ccggtcgtac cgtccgtggt
     2281 tgagctcttc ccgacggccg atttccagga gcgggaggtc catgacttct tcggcatcgt
     2341 cttcgaaggc caccccgacc tgcgccggat cctgatgccc gaggactacg tcggctggcc
     2401 gcagcgccgg gactttccgg tcggtggcga gccggtgatc ttcacccgtg acgaagtgac
     2461 caacccgggc tggtggcagt gatggcgcgc gtcgaagaca cacttcacga ccgtgaaatc
     2521 cggcaggccg gggcgatcgg cgaggccggc accctgcgcg acgaagagat cacggtctcc
     2581 gacttcacga ccgaactggc cgagcggatc cccggagtcg agtttgaact ggaacccgag
     2641 caggagatgc tcacggtcaa catgggcccg catcaccccg cgacccacgg cgtgctgcgg
     2701 ctcgtggtcg atctcgaggg cgaggtggtc aaggacctga agccggtgat cggctacgtc
     2761 cacaccggca tcgagaagtc ctgcgagacc atgcagtact ggaaggtcat ccccttcgtc
     2821 gagcggatgg actacctctc ctacttcttc aacatgcagg cctacgtggg ggcggtcgag
     2881 aagctgatcg gcctcgaggt tccccgccgc gcgcaatggc tgcgggtgct ccacatggag
     2941 ctgaaccgga ttcactcgca tctggtctgg ctcggcacct cggctctcga cctcggtgcg
     3001 atgggcgtct acttctattg cttccgtgac cgcgaccggg tacttgacac cttcgagatg
     3061 tcgaccggcc agcgcatgca tacccgctac ttccagatcg gcggggtagc cgaggacatc
     3121 ccggccggtt tcgagcagaa ggtgcgaaag acgatcgaac agctgaagac cggcatcggc
     3181 cagtacgaag cgctactcga caagaacgag atcttcctga tccggaccaa ggacgtcggc
     3241 gtcgtctcgc gcgagcgact ggtcgagctg ggcgtgaccg ggccgctgct gcgcgctgcc
     3301 ggcgacccct gggacctccg caaggaaggc ggctacctcg cctatccgga actcgaattc
     3361 gacgtgccgg tcggcacggt cggtgatggc tacgaccggt accgggtccg cctgaccgag
     3421 atgcgcgagt cgatcaagat catcgagcag atcctcgacg ggttgcccga gggcccctgg
     3481 atcgccgatg accgcaagta cgcgctgccg ccccgggccg agctttcgac ctcgatggaa
     3541 tcgctgatcc accacttcaa gctggtcacc gaaggcttcc gggtcgagcc gggcgaggtc
     3601 tacaacccga ttgaatcgcc ccgcggcgaa ctgggctgct tcgtgatggc cgacggctcg
     3661 tcgaaacccg cccgggtcca tttccgcgag ccttccttcg tcaaccttca ggccctggcc
     3721 gacatgacga tcggcgaata cgtggccgac ctgatccaga ccctggccat gctcgacccg
     3781 atcctgggag gtatcgaccg atgacccccg tgatgccgtc ggatttcgac ccccgcgacg
     3841 ccgagccgga ggatttcgtc tcacccggcc tgccggtcga tcgggcgaac accaccggac
     3901 gcctcccggt cggtgatccg gaacagattc cggacccggc cgaagtcgag gtgccggagg
     3961 acctgcgcga gcaaattcag gcgattgtcg acaagtaccc ggaccgggac cacaagggct
     4021 ccccgaagtc ggctgcgatc ccggtgctct ggatcatcca gcgccgttac ggctggtgca
     4081 ccccggaggg gatcgtccag gccgcctgcg tgatgcaggt gaccccggcc tacctcgaat
     4141 cggtcgcctc cttttacgac cttctgagga ccagccccca ggggagccat caggttctcg
     4201 tctgcaccaa cctcgcctgc tggatgcggg gcggcgatga gctgctcgac tccttctgcg
     4261 agaagaccgg ggcggaccgc gaagcggccg gccacggagg gtccctctcc gaagatggcg
     4321 agatcctggt ctccgggttc gaatgcctcg gtgcctgcga cctcgccccg atggcctcgg
     4381 ttgacgagcg ctacttcgga cctttggaga acgaggacgc ggccaccatc gtcgatcagc
     4441 tcgaatccgg tgaggaagtc cttccggaga aggcgctgga caaacgcggc ctggccggcg
     4501 gcgtcgccga ttcgaccgac ccccgggtga ccggcggaaa ggggccgaat cagtgagcgt
     4561 cgctgaaacc agagtgctgc tccgccacgc cgaagacccg aagatggcga ccctcgaggg
     4621 ttacgagtcc tacggcggct acgcgaccct gaagaaggcg ctgaacgaga tcgaccccga
     4681 gtggatcgtg accgagctcg aggaatcggg cctgcgcggt cgcggcggtg ccggattctc
     4741 aatgggcaag aaggtgtcct tcctgccgcg tacagaggat gccaagtacc tctgctgcaa
     4801 cgccgacgaa tccgagccgg gggccttcaa ggatcgcgag ctgatgcagc gaaacccgca
     4861 tcagctgatc gaaggcatca tcatctccag tttcggggcc aacatcggcc acgctttcat
     4921 cttcatccgc ggcgagtacg acgatcaggc cgacatcctc gatcgggcgg tcgatgaggc
     4981 ctatgaggcg ggttacctcg ggaccaacat cctcggttcg ggcttcaacc tcgagctggt
     5041 cgtccaccgc ggtgccggcg cctacatctg cggcgaggag accgccctgc tcgacgcgct
     5101 ggaaggcaag cgcggcaacc cgcgcctcaa gcctcccttc cctgcggtcc agggcctcta
     5161 cggcgggccg accctgatca acaacgtcga gaccctcagc aacgtgagcc acatcgtcgc
     5221 caacggcgcc gactggttca agagcttcgg caccgagcag tcacccggca ccaaggtcgt
     5281 ttcggtttcc ggctgcgtca aacgacccgg caactacgag gtcgagctcg gcgtcaccct
     5341 gcgcgacctg atctacggcc tggccggcgg cccgctcgac ggccacgaga tcaccgcctt
     5401 ctacccggga ggttcctcct caccggtcct gaccccggcc gaacttgacc tgccctattc
     5461 cttcgaggcg atggccgagg ccgggtcgat gctcggctcc gggtcgatca tcgtcgccga
     5521 cgactcggtc tcgattccga ggatggcgct ccggaccgct cacttctacc gtcacgaatc
     5581 ctgcggcaag tgcacgccct gccgcgaggg caccaactgg accgagaaga tgctcgaacg
     5641 ggtctgtgcc ggcgaggcca ccccgatgga cctcgacatc atcgaatcgg tccagcacaa
     5701 catcatgggc cattgtctct gtgtcctcgg tgacgccatg gcgatgccgg tcagctcgat
     5761 ggtcaacaag ttccgcgacg aattcgagga gacgatcgca ttcgaagccg cgcgagcctg
     5821 ggacggttcc ctggactcgt ctgcttctcc cggtcgggat gttccgtccg gcgatgagcc
     5881 cgggcctggt ggcgagagtc ccgagaagat ccggcgggag cggttggctg gccgcgcgat
     5941 tccggacggg agcggtcgct gatgccggct ccgaagaaga atctgatcac ccttacggtc
     6001 gatggccggg agatcgaggc gatcgaaggc acgatgctgg tcgatgcggc caagcacggt
     6061 gacatcgaga tcccggtctt ctgttacgag ccgaagctcg gcgcgccggt cggcgcctgc
     6121 cgcatgtgcc tggtcgagat cgagggcatc ccgaaactcc agactgcctg ctcgacaccg
     6181 gtccgcgacg gcatggtcgt tcacacccgg accgaccagg tcaaggaagc ccagaacgcg
     6241 gtcgtcgagt tcctgctggt caaccatccg ctcgactgtc cggtctgtga caagggcgga
     6301 gagtgcccgc tccaggacat ctcgatgggc tggggccccg accgcagccg tgtggttgac
     6361 accaagcgac atttcgagaa accggtcccg ctctcaccac tgatcgcgat cgaccgtgaa
     6421 cgctgcatcc tctgttaccg ctgcgcccgt ttcagccagg aagtttccga ggacggccag
     6481 ctgcaactgc tcgaccgggg cgccgagtcc tacatcggca ccttcgatga ccggccgtac
     6541 atcgcgccct tccagggcaa catcaccgag ctctgcccgg tcggcgccct gacctcctac
     6601 acctaccgtt tccaggcccg gccgtgggac atcgagcagg gcggctcggt ctgcaccctc
     6661 tgcccgagcc aatgcaacgt cagcttcacg atccgcgacg agcaggtgaa gcgcgtgctc
     6721 gatcgtgaaa acgaccagat cgacgacggc tggctctgtg accgtggccg ctacggcttc
     6781 caggcgatgt attcgcagga ccgggtcatg aacccgctcc gttacatcga cggccacccg
     6841 gccccggccc ggtggagcga cgccctcgaa gcggcttcga tcggtctcgc cgccgccgaa
     6901 ggcaaggttg ccgccctggt cggagacgct tccaacgaag aggctttcat cctgcaggac
     6961 ctgatccgga acggcctcgg ctccgctgac atcgattcac ggccggctcc cacggtcggc
     7021 cgcgaccagc ggatccgcct cgatgaccct gccacccagg cccgggtcgt tgacatcgac
     7081 cacgccgatg tggtgctggt actgggcaac gacccgatcc actcggctcc ggcctttgac
     7141 ctgcgggttc gcaaggcggt ccgtcgcaac ggcaccgcgc tggcggtcgc caccgagcgg
     7201 ccttccgccc tcgacggcgg cgcctccgaa gtcgagcgca tcaagccggg tgacgcggcc
     7261 gggtaccttg cgaacctgct cgaccaggtc aagtcgggtt ccaccgacgg catcgccggc
     7321 ctgctcaagg attccggcca cacggtcgtg atctggggtg agcggatcgg ccgcggtgaa
     7381 aacggcccgg tcgcgatcga gtcgctgctc gagctggccg gcgtcctcga cctcggtggt
     7441 caggaggaat cgggcctgat cgagattccc gatgtcgcca acgcccgcgg tctgcgcgag
     7501 gtcggctgcc ttccggacgc gggccccggc ctctcggaaa ccgaagtcgg tcgtgacgtc
     7561 gaggaaatca aggcgggcct gatctcgggc gaggttcgcg cggtcatcct ttacggagtt
     7621 gacccgctcc gcgacttccc cgacaccgag ggctggcgcc aggccctcga atccgccgac
     7681 ttcgtgatcc acttcacgat ggcttcgacc gacacgaccg agttcgcgga tgtggtcttc
     7741 ccgctcgaat cgcacgccga gaaggacggc acgatcaccc acaccgaagg ccgtctccag
     7801 cgggtccgtc ccgccgccgc ccgccccggg gacatccgcg gcggctggca tgtcctcgcc
     7861 gagctctccg ccgcactggg tcacgaaacg aatattcacg cagtccccga ggcccttcgc
     7921 gctatcactg aagttgtccc gttttacgcg ggactcaccg atgccgaaat cggcggccgc
     7981 ggaatcaggt ggcaggaccg ttccgaatca aatggttggg cggagtccgt ggggagcacc
     8041 ccggaccgga cttcgccggg agtttccggg gtacccccca cggactccac caccgagacc
     8101 gaggcggtcg tcgttggggg ttccgatgga actcccggcg aagtccggcc agcggaaccc
     8161 ccaacgtcgg ccgcccccac cgacgggttg acccttggta cttatcgcga tctgtgggct
     8221 aacagcacta cggacctgag ccccgccttg agttacttga ttccggctca gaggctggaa
     8281 gtgtcagttg cggatgccga gcgcctgggg ctgtccaagg gtgatgaggt ttccgtgaag
     8341 tcgggggata ccgaggtgac ggcgttcgtg gcgattcggg cggcgctgcc tgaaggcacc
     8401 tgcttcctga tggaggggac ccagcagggc aacgcgaacg tatttgccac cggaaagccc
     8461 gcagtcgttg aaatcggcaa ggcctctccg aagctggaag tcgtggccgg caacgggaac
     8521 gggctcgaca atctcgtcac cggcaacggc gccg
//
LOCUS       DAOQKN010000100         8519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_3988_length_8519_cov_5.755671, whole genome shotgun sequence.
ACCESSION   DAOQKN010000100 DAOQKN010000000
VERSION     DAOQKN010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 8519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8519
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3988_length_8519_cov_5.755671"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1995
                     /locus_tag="PLJ64_07750"
     CDS             <1..1995
                     /locus_tag="PLJ64_07750"
                     /inference="COORDINATES: protein motif:HMM:NF040071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6351 family protein"
                     /protein_id="HNC93412.1"
                     /translation="VADPAALKTAYEMGQLNEGSAGYASTPAIDNRQYWIETVPNGHQ
                     IIHALSMQQRLEESTGAPVPHFIWTSLYGPGDGDGNDDPTVPNNVTWGILGDWVDAIQ
                     ADTSDTDPQAKAVAHAPAAATDRCFNDDGSVLAIEPQTLNSGICGSTFPSHETPRMIA
                     GGPIANDVLKCALKPVDPSDYDGKLDAGQLAELESIFPTGVCDWEEPGVEQTYNTRTW
                     RAWPTGQADNSAPSTTISSSPRPSNAPATVDFEFSSSETGSQFECRLDDGGNTAGYED
                     CASPMRYGPLGDDLYTFSVRATDAAGNTGGPATVKFRTGGPAPVLAISPAVFAFGPIN
                     VGGELGEPGEFTLSNPGSDPVNLDDGTLTGPDAGSFKLLPNGSTCYFLEPAGVLPPGG
                     SCTLVAIFDPESEGPKTASLEVTSDSVTSPDQLSLSGTGLPELLPGASVTPGTLAFGN
                     RQAGTDSPARTITLTSTGSGDLEIGNLIRLGADASQFAVTGNTCSGRSMASGDSCQVQ
                     VVFSPVGTGARSATLTIPTDASGADTSVDLTGTATAPPVVHRNSRVQIGGSLPRLRGS
                     RQLRIPVRCFTTNMEFCRGRLRVKARGRALGLRHNRLVQVATDTYELRAGQRTVRVRI
                     GNRARRRVLRRGSLPVRVIGTSRQGNGSFRSVNAGRRLRR"
     gene            2054..3550
                     /locus_tag="PLJ64_07755"
     CDS             2054..3550
                     /locus_tag="PLJ64_07755"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNC93413.1"
                     /translation="MRRTGGILAAILIVGAIAAVPASGANAKPRAVVPSTSTIFGYIK
                     DLTSFGYRRTGSKASLKAATYVRDSFRRFGLSEVHFQTARSYAWKVSDSSLSVDGIKR
                     DSFPIAHAFMGGEQTGTFGTGPAGITAPIVDVGNGSASTFASKDVSGKVVMFDLRFLG
                     LPADVFKAISKFTYDPDNTVQPGEVIPQPYISNIVDVVQRAMDGGAVGVVGVLADYFD
                     SKYYFNEEYRKLQMDIPGMWVTRSEGDAIRAQLSVSPDAEAKLVMNGEREAAPARNVI
                     GFLPGQSRTTLLISSHHDAVWDGAVEDGSGTSEVLALARYFGALKKKYRPMSLMFATM
                     DSHFTGYQGHAAFLKKYVENAPEAERPRVNFAIEHIARHGYVDENGKLKMTNLPEIAG
                     ILENVGEKPLNVIRKVIPKYGLDRTVVLPATFFGVSLPTDASGAYQEAVPVVSLISGP
                     IYLYDRQDTLDKVYKPHLRPMALAYKEMIARMSRMDPEDLPGDPPYKP"
     gene            3528..4796
                     /locus_tag="PLJ64_07760"
     CDS             3528..4796
                     /locus_tag="PLJ64_07760"
                     /inference="COORDINATES: protein motif:HMM:NF015541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HNC93414.1"
                     /translation="MTRLTSRSRITRSRIRVFCFGLAAALLLVLVPGGAAAKPARLPK
                     APAGKNFYSPPAKLIKGKPGTAIWIRKTTGLARPPLAGRVLTIAYRSTSIRDRATVMS
                     GTIALPKGEPPKGGWPIVTFGHVTTGSADDCAPSRVTAASTELERMVRSNEFVGYLLG
                     HGVAVARPDFEGIGTPGRHPYMIGRSLGRSMIDIIRAGRQLDIGLGRRWAAAGHSEGG
                     QAALFTAKIGPRRAPGLSFRGVSAFAPASHVSDFVELARSFDTSSPLTDGFSGLGALI
                     LEGAAIAEPSLWQEYRDGGLSAEALELMPDTQRLCLADLSGTGSFGAMPPGALEGPDG
                     PQVLPEVYAVLDRNDPRTLSLPGVPVRLDQGSKDLVVWQSQTAEVADVLESKGAEVSY
                     EVWPGADHTDITDLDYAASASADWLIDHLR"
     gene            complement(4988..5335)
                     /locus_tag="PLJ64_07765"
     CDS             complement(4988..5335)
                     /locus_tag="PLJ64_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNC93415.1"
                     /translation="MGQHFLKPLFDRVVIKELEPDRMRNSGLVVPPGTSEPPPQHGIV
                     LAVGQGIDWWEAAGVRMPVRPGDHVVFPAQAGNWVEVDEERLLVCRVTELLGVLENAR
                     DPETPELGGEVLD"
     gene            5424..5999
                     /locus_tag="PLJ64_07770"
     CDS             5424..5999
                     /locus_tag="PLJ64_07770"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNC93416.1"
                     /translation="MDDDLTRGDGEDEGLSRRPDPEDLDPTRIKLRPVAVDHVGDLRW
                     IHERPEVSRWWDEPGEDFPWDEPDSTRLTIFVDGQIGGMIQYWEEDEPKYRHAGIDLF
                     IDPALHNKGIGTTAVREVARYLFEARGHHRLEIDPAAENLAAIRVYEKVGFKPVGLTR
                     RAERDADGGGWHHSLLMDMLSTELLPAEQED"
     gene            complement(6018..8297)
                     /locus_tag="PLJ64_07775"
     CDS             complement(6018..8297)
                     /locus_tag="PLJ64_07775"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HNC93417.1"
                     /translation="MGLCAGGVREGCRTVVAALALLAFTVALAGPARADETVFGGELT
                     CVTQPGGVRQCAGPGGPDTNAPADTVPSFDGTPIDINFALPDKSRFGPPYPLAMYFHG
                     FGGEKEPFGGYLKRFTDRGIAALGMTERGFKESCGSEVAIEALDADTPGACDEGFIHL
                     MDSRYEVRDAQYFAGILADEGLIHPQKIGVVGGSYGGGKALALATLKNRVMLPDGSLV
                     PWKSPAGKSMAIAVAAPIVPWSDMAYALAPNGGTLDYASSSPYRKPYGVMKSGIVNGL
                     FATGENFSGSYGAPVDPLFDVIGWKDELAAGEPYGNDPLIATAVGELTRFHSAGYIDD
                     SVTPAPMFIAQGLTDDIFPVDEAIRYYNRIKASHPDARIGLYLADIGHPRALLATQGR
                     PADIQIADARVEEWFAHYLLGDGPEPETTVVARSQVCPYEEPSGGPFTADTWAKLAPG
                     EVRISDPGRHVIEATSGDDGVAAGFISILPGCSQMPDTAEPGTWSRDFPAPGGDGYTV
                     AGSTTVIADISSPNGGESQIAARLLEVTGGQERLIGRALYRPARSGRQVFQLHANVYA
                     IGPDAHLRLQLLPRDGMTGPSAAASYGRPSSDQRDITISDIEVRVPVAEHPGEAGGQV
                     RQPLRKVLPAGRSLAAQFRPTGAARPTGAAQVDRLRIAGRRLSLRVRCRADTDRCLSG
                     RLVIRGYRRGGPVDRGLIASHRLPRMVTGHGRTYRLPLQRKLLERLRRLDNEKVRVRV
                     TVTVPGSREESTLLIFLAG"
BASE COUNT         1439 a         2907 c         2822 g         1351 t
ORIGIN      
        1 gttgccgatc ccgctgcgct gaagaccgcc tacgagatgg gccagctgaa cgagggctcg
       61 gccggatacg cctcgactcc cgccatcgac aaccggcagt actggatcga aacggtgccc
      121 aacggccacc agatcatcca cgcgctctcg atgcagcaaa ggctggaaga gtcgaccggt
      181 gcgccggttc cgcatttcat ctggaccagc ctctacggcc cgggggacgg cgacggaaat
      241 gacgacccga ccgtgccgaa caacgtgacc tgggggatcc tcggcgactg ggttgacgcg
      301 attcaggccg acacctcgga caccgacccc caggccaagg ctgtcgccca tgccccggcc
      361 gccgccaccg accgttgctt caacgatgac ggcagcgtgc tggcgatcga accccagacc
      421 ctgaactcgg gcatctgcgg gtcaaccttc cccagccatg aaacgccaag gatgatcgcc
      481 ggcggtccga tcgcgaacga cgtcctcaaa tgcgcgctga agccggtcga cccgtcggat
      541 tacgacggca agctcgacgc cggccagctc gcagagctcg agagcatctt cccgaccggg
      601 gtctgtgact gggaagaacc cggggtcgag cagacctaca acacccggac ctggcgggcc
      661 tggccgaccg gacaggccga caacagcgct ccctcaacca cgatctcttc gtcgccgcgg
      721 ccatcgaatg ccccggccac agtcgacttc gagttctcgt caagcgagac cggttcgcag
      781 ttcgagtgcc gcctggatga cggcggcaat accgccggct acgaagactg cgcctcaccg
      841 atgcgttacg gcccgctcgg ggacgacctc tacaccttct ctgtccgggc gacggacgcc
      901 gccggaaaca ccggcggtcc ggccacggtg aagttccgca ccggtggccc ggctccggtg
      961 ctcgccatca gcccggccgt gttcgccttc ggcccgatca acgtcggcgg tgagctggga
     1021 gagccgggtg aattcacgct cagcaacccg ggaagcgatc ccgtcaacct agatgacggg
     1081 accctcaccg gacccgacgc cggttccttc aagctcctgc ccaacggcag cacctgctac
     1141 ttcctcgagc cggccggggt tctcccgccg ggaggttcct gcacgctcgt ggcgatcttc
     1201 gaccccgaaa gtgaagggcc gaagaccgcc agcctcgagg tcacctccga ttcggtcacc
     1261 tcccccgacc agttgtccct gtcgggaacc ggccttcccg agctgcttcc cggtgcctcg
     1321 gtcacgcccg ggacgctggc cttcgggaac cggcaggcgg gcaccgactc tccggcccgg
     1381 acgatcaccc tgaccagcac cggcagcggc gatctcgaga tcggcaacct gatcaggctc
     1441 ggcgccgacg ccagtcagtt cgcggtcacc ggcaacacct gctcgggccg ctccatggcg
     1501 agcggtgatt cctgccaggt ccaggtcgtc ttctcgccgg tcgggaccgg ggcgaggagc
     1561 gccaccctga ccattccgac cgacgcctcc ggggccgaca cctcagttga cctgaccgga
     1621 accgcaacgg caccgcctgt ggttcaccgc aacagccgcg tccagatcgg tggcagcctg
     1681 ccgcgcctgc gtgggtccag acagttgagg atcccggtcc ggtgcttcac gaccaacatg
     1741 gaattctgcc gcggaaggtt gcgggtgaag gccaggggcc gtgcgctggg gctgcgtcat
     1801 aaccggctgg tccaggtcgc gaccgataca tacgagctgc gggcaggtca gcggacggtc
     1861 cgggtgagga tcggcaaccg ggcccggcgc cgggtgctcc gccggggcag cctgccggtg
     1921 cgggtgatcg gtacttcgcg ccagggcaac ggcagtttcc ggtccgtgaa cgccggccgt
     1981 cgcctgaggc gctgaaactc gaacctcccg gttgaccggc ggggtctatc cttgtcggca
     2041 tggaggaggg agcatgaggc gaaccggagg gattcttgcc gcgatcctga tcgtcggggc
     2101 aatcgctgcc gtgccggcat cgggagcgaa cgcaaagccc agggcggtgg tgccttcgac
     2161 cagcacgatc ttcggctaca tcaaggacct gacttccttc gggtaccggc gcaccggttc
     2221 gaaagcgagc ctcaaggccg cgacctatgt gcgggacagt ttccgacgat tcggtctttc
     2281 tgaagtccac ttccagaccg cccgctccta cgcctggaag gtcagcgact cctccctttc
     2341 ggtggacggg atcaagcggg acagcttccc gatcgcccac gccttcatgg gtggcgagca
     2401 gaccggaacc ttcggcaccg gcccggccgg gatcactgct ccgatcgtcg atgtcggcaa
     2461 cggttccgcg tccaccttcg cgagcaagga cgtgagcggc aaggtggtca tgttcgacct
     2521 ccggttcctc ggattgccgg ccgatgtctt caaggcgatc tccaagttca cctacgaccc
     2581 ggacaacacc gtccagccgg gcgaagtgat cccgcagccc tacatttcga acatcgtcga
     2641 cgtggtccag cgggccatgg acggtggcgc ggtgggcgtg gtcggggtgc tggccgatta
     2701 cttcgactcg aagtactact tcaacgagga ataccgcaag ctgcagatgg acatcccggg
     2761 catgtgggtg accaggtcgg aaggggatgc gatccgcgcc cagctttcgg tttctcccga
     2821 cgccgaggca aagctcgtca tgaacggcga gcgcgaggcg gccccggccc gcaacgtgat
     2881 cggcttcctg cccgggcaga gcaggacgac cctgctgatc tcttcgcatc acgacgcggt
     2941 ttgggacggc gcggttgaag acggctcggg cacctcggag gtactcgcgc tggcccgcta
     3001 cttcggggcg ctgaagaaga agtaccggcc catgtccctc atgttcgcga ccatggattc
     3061 ccatttcacc ggctatcagg gccacgccgc gttcctcaag aagtacgtcg agaacgcgcc
     3121 ggaagcggaa cggccgaggg tcaatttcgc gatcgagcac attgcccgcc acggatacgt
     3181 ggacgagaac ggcaagctga agatgaccaa cctgccggag atcgccggca tcctcgaaaa
     3241 cgtcggcgag aagcctctga acgtgatcag gaaggtgatc cccaagtacg ggcttgaccg
     3301 cacggtggtc ctgccggcaa ccttcttcgg ggtgtcgctg ccgaccgacg cttccggcgc
     3361 ttaccaggag gcggtcccgg tggtcagcct gatcagcgga ccgatctacc tctatgacag
     3421 gcaggacaca ctggacaagg tctacaagcc gcatctgcgc ccgatggctc tcgcctacaa
     3481 ggagatgatt gcccgcatga gccgtatgga cccggaggat ctgcccggtg acccgcctta
     3541 caagccgtag ccggatcacc cgcagccgta tccgcgtttt ttgcttcggc ctggccgcag
     3601 ccctgctgct ggtcctggtc ccgggcggtg cggcagcgaa gcccgccagg ctgcccaagg
     3661 ccccggccgg aaagaacttc tacagcccgc ccgccaagct gatcaagggc aagccgggaa
     3721 cggcgatctg gattcgcaag accaccggcc tggcccggcc gccgctggcc gggcgggtgc
     3781 tgaccatcgc ctatcgatcc acatcgatcc gggacagggc cacagtgatg tcaggcacga
     3841 tcgccctgcc gaaaggcgag ccaccgaagg gcggctggcc aatcgtcacc tttggccacg
     3901 tcacgaccgg ttccgccgat gactgcgccc cgagccgggt cacggccgcc agcacggaac
     3961 tcgaacggat ggtccgcagc aacgaattcg tcggctacct gctcgggcac ggagtggcgg
     4021 tggcccggcc ggacttcgag ggcatcggaa ctcccggccg gcatccctac atgatcggcc
     4081 gctcgctggg ccggtcgatg attgacatca tccgggccgg tcgccagctg gacatcggcc
     4141 tgggcaggcg ctgggccgcg gccggccatt ccgagggtgg gcaggcagcg ctgttcacgg
     4201 cgaagatcgg cccccggcgc gcgcccgggc tttccttccg cggtgtctcc gccttcgcac
     4261 cggccagcca cgtcagtgac ttcgtcgagc tggcccgctc ctttgacacc tcctcgcccc
     4321 tgaccgacgg cttctccggc ctcggggcac tgatcctcga aggcgccgcg atcgccgagc
     4381 cgtcgttgtg gcaggagtac cgcgatggcg gcctcagcgc cgaagcgctg gagctgatgc
     4441 ccgacaccca gcgactttgc ctggctgacc tcagcggaac cggttcgttc ggtgcaatgc
     4501 cccccggtgc gctcgaggga ccggacgggc cgcaggtgct gcccgaggtt tacgcggtcc
     4561 tcgatcgcaa tgacccccgt accctgagcc tgcccggggt tccggtccgg ctcgaccagg
     4621 gatcaaagga cctggtcgtc tggcagtcac aaactgcgga ggtcgcggac gttcttgagt
     4681 caaagggggc ggaggtcagt tacgaagtct ggcccggtgc cgatcacacc gacatcaccg
     4741 atctcgacta tgcggcgtcc gcctctgccg actggctgat cgaccacctt cgttgaggcc
     4801 tcaaccaatc gcgaacttct ttgtttgcgg gaccgaaact cccgaaccct tatccggtta
     4861 taagagcggt tgtctgacct ctggaaagac agccgggttc caaacgggag ggaaccaaca
     4921 agcagggagg cgctgagccc gatccggggg ggatcgggct caagcgtttc tctgctctcc
     4981 cgtgggttca gtcgaggact tcgccgccga gctccggggt ttccggatcc ctggcgttct
     5041 ccagaacacc cagcagctcg gtgacccggc agaccagcag ccgttcttcg tcaacctcga
     5101 cccagttgcc ggcctgggcc ggaaagacga catggtcacc ggggcggacc ggcatcctca
     5161 ccccggccgc ttcccaccag tcgatgccct gaccgaccgc cagcacgatc ccgtgctggg
     5221 gtggcggctc ggaggtgccc ggcggaacga ccagcccgga gttccgcatc cggtccggct
     5281 cgagttcctt gatcaccacc cggtcgaaga gcggcttcag aaaatgctga cccatgaccc
     5341 aagcctacgg gctgccggaa attcgggttg cgaagcgggc aggcccggtt cccagaggcc
     5401 aataccggta gcctgcggcc atcatggatg acgaccttac ccgcggggac ggggaggacg
     5461 aagggctctc gcgccgcccg gacccggagg acctcgaccc gacgagaatc aagctcaggc
     5521 cggttgccgt ggaccatgtc ggtgacttgc gctggattca cgaacgcccc gaggtctcca
     5581 ggtggtggga cgagccgggc gaggactttc cctgggatga acccgattcg acccggttga
     5641 cgattttcgt tgacggccag atcggcggga tgatccagta ctgggaagag gacgagccca
     5701 agtaccggca cgccggcatc gacctcttca tcgatccggc gctgcacaac aagggcatcg
     5761 gcaccaccgc ggtccgggag gttgccaggt acctgttcga ggcccggggc caccaccggc
     5821 tcgagatcga cccggcggcg gagaacctcg cggcgatcag ggtgtacgag aaggtcggct
     5881 tcaagccggt cggcctgacc cggcgggccg agcgggatgc cgacggcggg ggctggcacc
     5941 acagcctgct gatggacatg ctctcgaccg agctgctgcc ggccgaacag gaagactgac
     6001 cgccggcgcc tctggcgcta cccggccagg aagatgagca gggtgctctc ctcccggctg
     6061 cccggcacgg tcacggtcac cctcaccctg accttctcgt tgtccaggcg gcgaagccgt
     6121 tcgagcagct tccgctggag cgggagccgg taggtcctgc cgtggccggt caccatccgc
     6181 ggcagccggt ggctggcgat cagaccgcgg tcgaccgggc cgccacggcg gtaaccgcgg
     6241 atcaccagcc ggccggacag gcagcgatcc gtgtcggcac ggcagcgcac cctcaagctc
     6301 agtcgacggc cggcgattcg cagccgatcg acctgcgccg caccggttgg cctggccgcc
     6361 ccggtcggcc ggaactgggc ggccaggctg cggccggccg gcaggacctt ccgcagcggc
     6421 tgcctgacct ggccccctgc ctccccggga tgctcggcga ccgggacccg gacctcgatg
     6481 tcggagatcg tgatgtcccg ctggtcgctc gaaggccgcc cgtaggaagc cgccgcgctg
     6541 gggccggtca tcccgtccct gggcaggagt tgcagccgga gatgcgcgtc cgggccaatc
     6601 gcgtaaacgt tcgcgtggag ctggaacacc tgccggccgg agcgggccgg acggtagagc
     6661 gcccttccga tcaagcgctc ctggcctccg gtcacttcca ggagccgggc cgcgatctgt
     6721 gactcaccgc cgttcggaga ggagatgtcg gcgatcaccg tggtcgagcc ggcgaccgtg
     6781 tagccatccc cgccgggggc cgggaagtcc cgggaccagg tacccggttc cgcggtgtcc
     6841 ggcatctgcg agcagccggg caggatgctg atgaaaccgg cggcgacacc atcgtctccc
     6901 gaagtggcct cgatcacatg gcggcccggg tccgagatgc gcacctcgcc gggggccagc
     6961 ttcgcccagg tgtcggcggt gaagggtccg cccgagggtt cctcgtacgg gcagacctgg
     7021 gagcgggcga cgaccgtggt ctccggctcg ggtccgtcac cgagcaggta gtgggcgaac
     7081 cattcctcga ccctggcgtc cgcgatctgg atgtctgcgg gcctgccctg ggtggccagc
     7141 agggcccggg gatgaccgat gtcggcgagg tagagcccga tcctcgcgtc ggggtgcgag
     7201 gccttgatcc ggttgtagta gcggattgcc tcgtctaccg ggaagatgtc gtcggtcagt
     7261 ccctgggcga tgaacatcgg ggccggggtc accgaatcgt cgatgtagcc ggccgaatgg
     7321 aatctggtca actcgccgac cgcggtggcg atcagcgggt cgttcccgta gggctcgccc
     7381 gcagccagct cgtccttcca gccgatcacg tcaaagagcg gatcgaccgg ggctccgtaa
     7441 ctgccgctga agttctcgcc ggtagcgaac aagccgttga cgattccgga cttcatcacc
     7501 ccgtaaggct tgcggtaggg agagctgctc gcgtagtcga gggtgccgcc gttcggagcc
     7561 agggcatagg ccatgtcgga ccagggcacg atcggggcgg ccaccgcgat cgccatcgac
     7621 ttgccggccg ggctcttcca cggcacgagg ctgccgtccg gcagcatgac ccggttcttc
     7681 agcgtggcca gcgccagcgc cttgcctccg ccgtaggacc cgccgaccac cccgatcttc
     7741 tgcggatgga tcaggccctc gtcggccaga atcccggcga agtactgggc atcgcgaact
     7801 tcgtagcggc tgtccatcag gtggatgaag ccctcgtcgc aggccccggg cgtgtcggca
     7861 tcgagcgctt cgatcgcgac ctcgctgccg caggattcct tgaatcccct ttcggtcatg
     7921 ccgagtgcgg cgatcccgcg gtccgtgaat cgtttgaggt agccgccgaa gggctccttc
     7981 tcgccgccga aaccgtggaa gtacatggcc agcggatagg gcgggccgaa cctggacttg
     8041 tcgggcaggg cgaagttgat gtcgatcggg gtcccgtcaa agctcgggac cgtgtcagcc
     8101 ggcgcgttgg tgtccggccc gccgggaccg gcgcattggc gcactccgcc gggctgggtc
     8161 acgcaggtca gctctccccc gaacacggtt tcgtcggccc gggcggggcc ggccagcgcc
     8221 accgtgaagg caagcagcgc cagggcggca acaaccgtcc ggcatccctc ccgcactcct
     8281 cccgcgcaca ggcccatgcc gcaattctag acctgaggga agggcattca ggtgtacttg
     8341 ggtaatcatt gggtatcccg actgaggatt ttgtcgatgc gtcccccaag aaagcccacc
     8401 agaagacgcg gtcccgaccc tgcccccgag gacacgcagg agatgttcgc cgcagggcag
     8461 gtcgtgcccg gggaacccgc cggggacgag gaaaccaggg agtacgaaac cgtcgcgac
//
LOCUS       DAOQKN010000101         8518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_3989_length_8518_cov_6.289968, whole genome shotgun sequence.
ACCESSION   DAOQKN010000101 DAOQKN010000000
VERSION     DAOQKN010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 8518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8518
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3989_length_8518_cov_6.289968"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1109
                     /locus_tag="PLJ64_07780"
     CDS             <1..1109
                     /locus_tag="PLJ64_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HNC93418.1"
                     /translation="KDLVVCSVLSGNRNFEGRISQDVKANYLASPPLVVAYALAGRMD
                     IDIQKDPLGNDADGNPVYLDDIWPDPAEVAQVVGDSIRREMFEKNYGDVFKGDENWQK
                     VSVPEGDRYTWPDSTYVRKPSFFEDMPADPPGLMPIEGARVLARLGDSITTDHISPAG
                     AIKKDSPAGAWLIEQGVGPADFNSYGSRRGNHEVMVRGTFANIRLRNQLVEKSGGFTR
                     HFPDGEETSIYEASSQYQADGIPLVVLAGKEYGSGSSRDWAAKGTRLLGVRAVIAESY
                     ERIHRSNLIGMGVVPLQYPDGESTDSLGLTGEEVITIGDLKGGEAKTVEVTAEREGAD
                     PVTFTATVRLDTPNEIRYINNDGILQTVLRDLNA"
     gene            complement(1131..3083)
                     /locus_tag="PLJ64_07785"
     CDS             complement(1131..3083)
                     /locus_tag="PLJ64_07785"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HNC93419.1"
                     /translation="MPVSVSLEGQPYSASWPDSASFTARLKVEAGSQGADFGFQLDPT
                     GWGLEGVGFGSVFRDGQAVLDGPGELTSNPIGSGGPSGLPGYCSRGGQVPNFDYRLKL
                     LPDTSTVVEIPVSLNASPLEGMNSDLRIKYYASSPTGISPTIDAPVQVGGKTGVLVRF
                     DTGSSGQSPLTANNREPIRLSGSTAPSLPNTEILIKATPFAASQPVPPVRSISVTTDA
                     EGNFSREVVFDRSAFWRLTAHPMVDSSHDDEPSCGPTLVTTVDTYGDLPAEAGLSASA
                     PVSWPDQKDFEYRLSLTAGDHGADFNFEFQYSDWGLSGVSGTPFSIGEASLEGPGSLT
                     TVSTSVADPLPTACWRGGFGSVFNTYNLKLDPNQETVVTVPATLLAPRLAGMKPEIGA
                     VLSAGDGEPQELTAPVTITGEEGIRIIARVAGATGSVAALAPGLPFRLVGRTVPTVKN
                     RLIRLRVEPRIWEFGRLSPAPVAHRIAVVRTDGNGRFRSQPVTLGREAQWVLTSRLLN
                     AGEFDNRPNCNGTLDSAGSAPTTGELIGRSYRSVAVRGTKLKKPLKISFATGPTMRRD
                     ENGKQIEGPLLLAYVDCNGMGGEYKVRDGRLTWFSVYSTAMYCPGSKDGWLLGLLGRG
                     ARARTNGSLLTLSRGNQRLVLRETGQ"
     gene            complement(3672..7991)
                     /locus_tag="PLJ64_07790"
     CDS             complement(3672..7991)
                     /locus_tag="PLJ64_07790"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HNC93420.1"
                     /translation="MAEATAVEVTMPEMGESVTEGTVLEWHVAVGDTIEEGETLVEVS
                     TDKVDAEVPAPVTGTVTELRAEVDDEIAVGAVLAVIDPDAGGSGPSTTVAAGEGDADL
                     GDSATPKSSSAPNGSAAADDSTSSNGASADPIEVTMPEMGESVTEGTVLEWHVAVGDT
                     VEEGQTLVEVSTDKVDAEVPAPTAGEITELLVEPDQEIPIGAALCKMIPGAGSGGAGN
                     GSAPAAAEGAAPAAPGATDAGDGRATPVAKRVAAAKGLTLGQIAGSGPGGKVTKADVL
                     AAGDGSGAVANREEKALRGPAGMLAKAMDESRSIPTATSFRTVAVDTLDAKRKGLNGA
                     LAERGMKVSFTHLVAWAIVRAARKWPVMARHFEEKDGKLFSVSDGQVNLGIAVDVEKK
                     DGSRSLMVPAIKGADQLDFPAFHAYYEDLITKTRENRLTPDDFAGTNITLTNPGGIGT
                     VASVPRLMSGQGTIVAAGSIAYPPEWSHADQEKIEQLGVSKVMTLTSTYDHRIIQGAE
                     SGNFLKTLDGLLAGGDEFYELVADDLGVDRTVLTAAQAKAKQAPPLSAAAAPAASAIA
                     EAPSHELLQAVQAATSLLKAYRTHGHLAAHLDPLGAKPKGDPALDPETVHLTPELMAQ
                     VPASILRIGVPGETLLEALPRMKAAYTGSIGYQFEHISSHSQRVWLREMVETGAHRKA
                     LEQDEQKRLLGRLIDVFEFERFLEKAYLGQKMFSIEGLDSVVTMLDELTTLAARGGAK
                     EVVLGMAHRGRLSVLAHTVRRPAEAIFAEFEGGKRIEDVKAVAAIPHGGTGDVKYHYG
                     HEGVAEHHDGSKIKVHLYPNPSHLEFVNPVITGATRYSQDVIDGSELTHDPASAVPVV
                     LHGDAAFPGQGVVAETFNLQSLEGYSVGGTIHIIENNQIGFTTDPNEGRSTPYAADMA
                     KGFNVPIIHVNADDVEACSQAMRLAMAYRERWNRDIVIDVIGYRRFGHNETDEPAYTQ
                     PLMAAEIKKHKPVSEIYTEQLIEQKVVTADEVGAAAEKRRASLKAALDELRKKMDTGV
                     YEDPTVTQVGTGELDRSASPPVQTAVTADKLRDLNAALLEVPASFTIHRKLRKPLGRR
                     IEALEEGGVEFAHAESLALASLITEGRHVRMSGQDTERGTFSQRHLVLHDEKTGIKYA
                     PIQHLKDATAPFELHNSPLSEIACLGFEYGYASSNPDSLVIWEAQFGDFANSAQVIID
                     SFIASGEAKWGMTSRLTMLLPHGHEGAGPEHSSARMERFLQLAAEGSMRVANPSTAAQ
                     YFHLLRRQALIKKARPLVVFTPKGLLRQTAATATLEQLTGEHFHFILDDPKATERREK
                     VERLVLCSGRIYHDIDAAASREEAEKVAVARVELLYPFPREQLAELFKAYPNLKEVVW
                     VQEEPKNMGAWATMWRRLPPIMPEGVNLEYIGRPERSSPSEGYSVAHIREQERIVLTA
                     LTP"
     gene            8147..>8518
                     /locus_tag="PLJ64_07795"
     CDS             8147..>8518
                     /locus_tag="PLJ64_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNC93421.1"
                     /translation="MDYFITGGTGFIGRYLIERLLKRDGTVYVLVREGSRGRLEEMRS
                     GWGEEADRVVPVIGDLSQPNLGLDADKIAELKGKIDAFYHLAAIYDMEANEEAIRIGN
                     IEGTINAVDLANQLEVGCFNHA"
BASE COUNT         1401 a         2690 c         2896 g         1531 t
ORIGIN      
        1 agaaggacct ggtggtctgc tcggtccttt ccggcaaccg caatttcgag ggccggatca
       61 gccaggacgt caaggcgaac tacctcgcct cgccgccact cgtggtggcg tacgcactgg
      121 ccggccggat ggacatcgac atccagaagg acccgctggg caacgacgcc gacggcaatc
      181 cggtttacct cgacgacatc tggccagacc cggccgaggt cgctcaggtg gtcggcgatt
      241 cgatccgccg cgagatgttc gagaagaact acggcgatgt cttcaagggc gatgagaact
      301 ggcagaaagt ctcggtgccc gagggtgacc gctacacatg gccggattcg acctatgtcc
      361 gcaagccctc cttcttcgag gacatgccgg ccgacccgcc cggccttatg ccgatcgagg
      421 gagcccgggt gctcgccagg ctgggcgact cgatcaccac cgatcacatt tcgcccgccg
      481 gagcgatcaa gaaggacagc ccggccggtg cctggctgat cgagcaggga gtcggcccgg
      541 ccgacttcaa ctcatacggc tcacggcgcg gcaaccacga ggtgatggtg cgtggcacct
      601 tcgccaacat ccgcctccgc aaccagctgg tcgagaagtc cggcggcttc acccgccact
      661 tcccggacgg cgaggagact tcgatctacg aggcttccag ccagtaccag gccgacggca
      721 tcccgctggt cgtcctggcc ggcaaggagt acgggtcggg ttcatcccgc gactgggccg
      781 ccaaggggac ccgcctgctc ggggtccgcg cggtcatcgc cgaaagctac gagcggatcc
      841 accgctcgaa cctgatcggc atgggtgtgg ttccgctcca gtacccggac ggggaatcaa
      901 ccgattcgct cgggctgacc ggcgaagagg tgatcacgat cggtgacctg aagggcggcg
      961 aagcgaagac ggtcgaagtg accgccgagc gggaaggagc cgatccggtc accttcaccg
     1021 cgacggtccg cctcgacacc cccaatgaaa tccgctacat caacaacgac gggatcctgc
     1081 agaccgtcct gagggacctg aacgcctgat ccggaaagat ctgcgccggc ctattggccg
     1141 gtttcacgca ggaccagccg ctgatttccc cggctcagag tcaggaggga accgtttgtc
     1201 cgtgcccggg cgcccctgcc cagcagaccg agcagccagc cgtccttcga gccggggcag
     1261 tacatcgcgg tcgaatagac gctgaaccag gtcagcctgc cgtccctgac cttgtactcg
     1321 ccccccattc cgttgcagtc gacgtaggca agcaacagcg gcccttctat ctgtttgccg
     1381 ttctcgtcgc ggcgcatggt cggaccggtc gcgaagctga tcttcagggg tttcttcagc
     1441 ttggttcccc tgactgccac cgatctgtag gagcggccga tcagttcccc ggtcgtcggt
     1501 gcgctccccg ccgagtccag ggttccgttg cagttcggcc tgttgtcgaa ctccccggcg
     1561 ttgaggagcc ggctggtcag gacccactgg gcttcccggc cgagcgtgac cggctggctc
     1621 cggaaccggc cgttgccgtc ggtgcggacg acggcgatcc gatgcgcgac gggtgcgggg
     1681 ctcagcctcc cgaattccca gatccgaggc tcgacccgca gccggatcag ccggttcttt
     1741 acggtcggca cggtgcggcc gaccagtcga aatggaagcc cgggggcgag cgcggcgacg
     1801 gacccggtcg ctccggcaac cctggcgatt atgcggatcc cttcctcgcc ggtgatcgtg
     1861 accggcgcgg tcagttcctg cggctcaccg tccccggccg aaagcacggc cccgatctcc
     1921 ggcttcatgc cggccaggcg gggagccagc agggtggccg gaacggtgac cacggtctcc
     1981 tggttcgggt cgagtttgag gttgtacgtg ttgaacacgc tgccgaagcc accccgccag
     2041 caggcggtcg gcaaaggatc ggcgacggat gtcgaaacgg tggtcaggga accggggccc
     2101 tcgagcgagg cctccccgat cgaaaacgga gttccgctta ccccgctcag gccccagtcc
     2161 gaatattgaa actcgaagtt gaaatcagcc ccgtgatcac cggcagtgag agatagccgg
     2221 tactcgaagt ccttctggtc gggccacgaa accggggcag aagcgctcag gccggcctcg
     2281 gcaggcaggt caccgtaggt gtcaaccgtg gtcacgaggg tcgggccgca actcggttca
     2341 tcgtcgtggg aggagtcaac catcgggtgg gcggtgaggc gccagaacgc gctgcggtcg
     2401 aagacgacct ccctgctgaa gttgccctcc gcgtcggtgg tgacgctgat cgagcggacc
     2461 ggcggaactg gctgcgatgc cgcgaagggc gtcgccttga tcaggatttc ggtgttcggc
     2521 agggacgggg cggtggatcc gctgagccgg atcggctccc ggttgttggc cgtgagcgga
     2581 ctctggccgc tgctcccggt gtcaaacctg accagcactc cggtcttgcc cccgacctga
     2641 accggcgcgt cgatcgtcgg ggaaatgccg gtcggcgagc tggcgtagta cttgatccgc
     2701 aggtccgagt tcatgccttc cagcggcgaa gcattgaggc tgaccgggat ctcgaccacc
     2761 gtcgaggtat cgggaagaag tttcagccgg tagtcgaagt tgggcacctg gccgccccgg
     2821 ctgcagtatc cgggcaaccc ggagggccca ccggagccga tcggattcga ggtcagctca
     2881 cccggtccgt cgagaaccgc ctgcccgtcc cggaacacgc ttccgaagcc gacgccttca
     2941 aggccccagc cggtcgggtc gagctgaaac ccgaagtcag ctccctgcga accggcttca
     3001 accttcaacc gggcagtgaa ggaagccgaa tccggccaac tggccgagta gggctgaccc
     3061 tcaagcgaaa cagaaacagg caccgttccc gcctgggcgg caaccggaag gacaccggcc
     3121 gcgagaagag cggcaaggaa aacaaaaaac cccctgaaag tcctcatgac aaaaaagagc
     3181 ataaacggga agccggtaac tggcgacaaa gttcagggtc tggtcagtgg tgccaaccag
     3241 cggagctggc ccatccgtca gtgaatgtga gtgggccggc gggtcaaaaa gaaagggccg
     3301 gcgtagaccg gcccggccac ctaaaaagtg gcgggcgagg gtcgcgggag gcagggggtc
     3361 cgcgcgaccc tcaccacgcc tgcgcgtcgg tagccaggga gggatgccga cgcgaacgct
     3421 tccgggggta aaccgccgga agcgggcttg gaaaatttcg actggtggca acccctccgg
     3481 ggcgctggtc cgatcctgct ccttcccttt tctccctact gcgactgcgg acctgcaacg
     3541 cagtttatac acctattgac gatgtgcgtc aacgaggaac gaatacgcag tccggtctta
     3601 gccggaatgc ggcggaaacc cgccgccccg gagagtcaaa tcgtcaccga ggcggccccg
     3661 gccgaggtgg ctcagggagt gagagcggtc aggactatgc gttcctgctc gcggatgtgg
     3721 gccaccgagt agccctccga cgggctggag cgttccggcc ggccgatgta ctcgaggttc
     3781 acgccctcgg gcatgatcgg cggcagtcgc ctccacatcg tcgcccaggc gcccatgttc
     3841 ttcggttctt cctggaccca gaccacttcc ttcaggttcg ggtaggcctt gaacagttcg
     3901 gccagctgct cgcgggggaa ggggtagagc aactcgaccc tggccacggc caccttttcg
     3961 gcttcctcgc gggaagcggc cgcgtcgatg tcgtggtaga tccggcccga gcagagcacc
     4021 agcctttcga ccttttcccg gcgttcggtg gctttggggt cgtcgaggat gaagtggaag
     4081 tgctcgccgg tcagctgctc gagcgtggcg gtggccgcgg tctggcggag caggcccttc
     4141 ggggtgaaga cgaccagtgg cctggccttc ttgatcagcg cctggcgcct gagcaggtgg
     4201 aagtactgcg ccgcggtgga cggattggcg acccgcatcg atccttcggc agccagctgg
     4261 aggaaccgtt ccatgcgggc gctcgagtgc tccgggccgg ctccctcgtg gccgtgcggc
     4321 agcagcatcg tgaggcgtga ggtcatgccc cacttcgctt cgcccgaggc gatgaagctg
     4381 tcgatgatga cctgggccga gttggcgaag tcaccgaact gggcttccca gatgaccagc
     4441 gagtcggggt tcgaagaggc gtagccgtac tcgaatccga ggcaggcgat ctccgagagg
     4501 gggctgttgt gaagctcgaa cggcgcggtc gcatccttca ggtgctggat cggcgcgtac
     4561 ttgatgccgg tcttctcgtc gtgcagcacc aggtgccgct gcgagaaggt gccccgttcg
     4621 gtgtcctggc cggacatccg cacgtgcctg ccttcggtga tcagcgaggc gagggcaagc
     4681 gattcggcat gggcaaactc aacgccgcct tcttcgagtg cttcgatccg gcggcccagc
     4741 ggcttgcgca gcttgcggtg aatggtgaag ctggccggaa cctcgagcag cgccgcgttc
     4801 aggtcccgca gcttgtcggc ggtgacggcg gtctggaccg gcgggctggc ggagcggtcg
     4861 agttcgccgg tgccgacctg ggtgacggtc ggatcctcgt agaccccggt gtccatcttc
     4921 ttgcgcagct cgtcgagagc cgcttttagc gaggcgcggc gtttctccgc ggcggcgccg
     4981 acttcgtcgg cggtgaccac tttctgttcg atcagttgtt cggtatagat ctcggaaacc
     5041 ggcttgtgct tcttgatttc cgcggccatc agcggctggg tgtaggccgg ctcgtcggtt
     5101 tcgttgtggc cgaagcggcg gtatccgatc acgtcgatca cgatgtcgcg gttccagcgt
     5161 tcgcggtagg ccatcgccag ccgcatcgcc tgcgagcagg cttcgacgtc atcggcgttg
     5221 acgtggatga tcggcacgtt gaagcccttt gccatgtcag ccgcatacgg ggtggagcgg
     5281 ccttcgttgg ggtcggtggt gaagccgatc tggttgttct cgatgatgtg gatggtgccg
     5341 ccgaccgagt agccctccag cgactggagg ttgaaggtct cggcgaccac accctgaccg
     5401 gggaaggccg catcgccgtg gagtacgacc ggaaccgcac tggccgggtc atgggtgagt
     5461 tcactgccgt cgatcacgtc ctgcgagtaa cgggtcgcgc cggtgatgac cgggttgacg
     5521 aactcgaggt ggctcggatt cgggtagagg tgaaccttga tcttgctgcc gtcgtgatgc
     5581 tcggccacgc cttcgtggcc gtagtggtac ttgacgtcac cggtgccgcc gtgagggatc
     5641 gcggccacgg ccttcacgtc ctcgatccgc ttgccgcctt cgaactcggc gaagatcgct
     5701 tcggccggtc tgcgcacggt atgggcgagc accgagagcc ggccccggtg ggccatgccg
     5761 agcacgactt ccttggctcc gccgcgggcg gcgagggtgg tcagttcgtc gagcatggtg
     5821 acgaccgagt cgaggccctc gatcgagaac atcttctggc cgaggtaggc cttctcgagg
     5881 aaacgctcga attcgaagac gtcgatcagg cggccgagca ggcgcttctg ctcatcctgt
     5941 tcgagcgcct tgcggtgggc tccggtttcg accatctcgc gcagccagac ccgctggctg
     6001 tgggaagaga tgtgctcgaa ctggtagccg atcgagcccg tgtaggcggc cttcatccgc
     6061 ggcagcgctt cgagcagggt ttcgccgggc acgccgatgc gaaggatcga tgccgggacc
     6121 tgggccatca gctcgggggt gaggtgcacg gtctccgggt ccagcgcggg atcgcccttg
     6181 ggtttggccc cgagcgggtc gaggtgggcg gcaaggtgac cgtgggtgcg gtaggccttg
     6241 agcagcgagg tcgctgcctg aaccgcctgg agcagttcgt ggctgggcgc ttcggcaatt
     6301 gcgctggcgg ccggcgcggc agcggccgag agcggaggtg cctgcttcgc cttcgcctgg
     6361 gcggcggtaa gtacggtgcg atcgactccc aggtcgtcag caaccagttc gtagaactcg
     6421 tcaccgccgg ccagcaggcc gtcgagggtc ttgaggaagt tgccggactc ggccccctgg
     6481 attatccggt ggtcgtaagt cgaggtcagg gtcatcacct tcgagacgcc gagttgctcg
     6541 atcttctcct gatcggcatg tgaccactcc ggcgggtagg cgatcgagcc ggcggcgacg
     6601 atcgttccct ggccgctcat cagccgcggc accgaggcga cggtgccgat cccgcccggg
     6661 ttggtcaggg tgatgttggt gccggcgaag tcgtccgggg tcagccggtt ttcacgggtc
     6721 ttggtgatca ggtcttcgta gtaggcgtgg aaggccggga agtcgagctg gtcggcaccc
     6781 ttgatcgccg ggaccatcag gctgcgggag ccatccttct tctcgacgtc gaccgcgatg
     6841 ccgaggttga cctggccgtc ggagaccgag aagagcttgc cgtccttctc ctcgaagtga
     6901 cgggccatga ccggccactt ccgggctgcc cggacgatgg cccaggcgac caggtgggtg
     6961 aaggaaacct tcatgccgcg ttcggcgagg gcaccgttga gccccttccg cttggcgtcc
     7021 agggtgtcga ctgcgacggt cctgaacgag gtcgcggtag ggatcgagcg gctctcgtcc
     7081 atcgccttgg ccagcatccc ggccgggccg cgaagtgcct tctcttcgcg gttggcgacg
     7141 gcgccgctac catcacctgc cgcgagcaca tcggccttgg tcaccttgcc accggggccg
     7201 ctgcccgcga tctggcccag ggtgaggccc ttggcggccg cgacgcgttt ggcgaccggg
     7261 gtggcgcggc cgtcgcctgc gtcggttgcg cccggggctg cgggcgcagc gccttcggcc
     7321 gcggccggag cggaaccatt gccggctcca ccggagcctg cgcccggaat catcttgcag
     7381 agtgccgcgc cgatcgggat ttcctgatcg ggctcgacca gaagctcggt gatctcgccg
     7441 gcggtcgggg cgggtacttc ggcgtcaacc ttgtcggtgg aaacctcgac cagggtctgg
     7501 ccttcttcaa ccgtgtctcc gaccgcgaca tgccattcga gcacggtccc ttcggtcacc
     7561 gattcgccca tctcgggcat ggtcacttcg atcgggtccg cggaagctcc gttgcttgag
     7621 gtcgaatcgt ctgcggcagc gctgccgttg ggggcacttg aagacttggg agtggcgctg
     7681 tcgccaaggt cggcgtcgcc ttctcctgcg gcgacggtcg tcgaagggcc cgagccgcct
     7741 gcatcggggt cgatcacggc gaggactgca cccaccgcaa tctcgtcatc cacttctgca
     7801 cgcaactcgg tcaccgtgcc ggtgaccggg gcgggcacct cggcgtcgac cttgtcggtc
     7861 gagacctcta cgagagtttc gccctcctcg atggtgtcgc cgaccgcgac gtgccactcg
     7921 agcacggtcc cttcggtcac cgactcaccc atctcgggca tcgtcacttc aacagcggtt
     7981 gcttcggcca tgtgttccaa gtgtatgtat ttgggcaagc cgcgtttcgg acatttggac
     8041 aagctgggtc aagaaatcca aaccggaatc gcttcgcctc gttttgacgg tggttgtagt
     8101 gtccccgcta gtccgctaat cgcgtcgatt gctcggagga ggagctgtgg attacttcat
     8161 caccggaggc accggtttca tcggtcgcta cctgattgaa aggttgctca agcgggatgg
     8221 aaccgtgtac gtcctggtgc gggaaggctc ccgcggtcga ctcgaggaga tgcgctccgg
     8281 ctggggcgag gaagccgacc gtgtggtccc ggtcatcggc gacctcagcc agccaaatct
     8341 cggcctcgac gccgacaaga tcgccgagct gaagggcaag atcgacgcct tctaccacct
     8401 tgccgcgatt tacgacatgg aagccaacga ggaagcgatc cggatcggca acatcgaggg
     8461 aacgatcaac gcggtcgacc tcgccaacca gcttgaggtc ggctgcttca accacgcc
//
LOCUS       DAOQKN010000102         8486 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_4016_length_8486_cov_5.469339, whole genome shotgun sequence.
ACCESSION   DAOQKN010000102 DAOQKN010000000
VERSION     DAOQKN010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 8486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8486
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4016_length_8486_cov_5.469339"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="PLJ64_07800"
     CDS             <1..370
                     /locus_tag="PLJ64_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93422.1"
                     /translation="PLAGEHNVLNALAASAIALALDVPLPTLKAGLEGAEHVKGRLVL
                     SGESTAARMSRIARAVLHDLPLLSLDEMLARVDAVSVEDVSALASELYAEERLSAASV
                     GRDEDRFRKAIQPVSEALAA"
     gene            367..1044
                     /locus_tag="PLJ64_07805"
     CDS             367..1044
                     /locus_tag="PLJ64_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HNC93423.1"
                     /translation="MIRVGVAGAAGRMGATVCGAVEEADGLELTGRADPALDTGLSAI
                     LPDADVVVEFTTPDTSLANSIACLEAGVHVVVGATGFDLDALRDAAENSGTGANCFVA
                     PNFAIGAVLLMELAQKAARHMPECEIIELHHDRKLDAPSGTAKRTEELITAAGGNVHQ
                     PIHSVRLPGLVAHQEVLFGGTGQTLSIRHDSIDRSSFMPGVVLAVRKVGQLEDRFTVG
                     LEKILDD"
     gene            1037..2491
                     /locus_tag="PLJ64_07810"
     CDS             1037..2491
                     /locus_tag="PLJ64_07810"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNC93424.1"
                     /translation="MTDVVALDATAQAELVAKGEVSPAELVAGAIERAEKVNPELNAI
                     CSPLFEQATEAAAGELPDGPFRGVPFLFKDLGAGLAGQPMHMGNRVLKEIGMTMPYDT
                     HLGSRFRDAGLVTLGRTATPELGILPTTEPVLNDGPTKNPWDTTRSSGGSSGGSAAAV
                     AAGIVPIAHASDGGGSIRIPASECGLVGLKATRARISQAPVMGDSMSGLTTELAVAHS
                     VRDIATMLDWVHGPAPGDPYGCPVPARPYSEEVTAGTEPLKIALLTESLTGDSLQPAV
                     VNAARETARKLEDLGHEVTEFDLPAAGEPDALYQTFVTRWAAGMSQTADTLSLIIGRP
                     LTSEDVEPLTWALIEKGRTEGGAAYLSAVAQHQLIGRLVAGVMLDGQFDLIMTPTLAT
                     VPPKLGHFDQNGPDPMDAMLKAREIATFTGIFNATGQPAISVPMEMSEEGLPIGIQFV
                     APMWGEHVLIQLAGELERAHPWIGRRAPGVLDAG"
     gene            2502..3371
                     /gene="dapA"
                     /locus_tag="PLJ64_07815"
     CDS             2502..3371
                     /gene="dapA"
                     /locus_tag="PLJ64_07815"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNC93425.1"
                     /translation="MSEIRGVITALATAFRPDGELDIDSTRKLAAYLVENGSHGLVVA
                     GSTGESSTLEDDEKIDLTKAVVEEVGPEVTVIVGSGSNDTRHSVELTKMAAEAGADAA
                     LIVTPYYVRPNRAGIRRHFEAVAEGSPGFPIVAYNIPSRTGINMPPEFLAELAQIDEV
                     KAVKQANDDDLGPIEGLDVLAGNDGTFLPTLKFGGAGGILVASHLDNGRMRTIWDLWQ
                     EGRHDEAEALDNQLQPLYEAMGVVVNPIPVKAALAARGLLPPTMRLPLVEPTDQERDA
                     VEELLAEAGVPAA"
     gene            3368..5032
                     /locus_tag="PLJ64_07820"
     CDS             3368..5032
                     /locus_tag="PLJ64_07820"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HNC93426.1"
                     /translation="MSQDRIKLLPLGGLGEIGKNMMVIEYRGSIVVVDTGLMFPTPEM
                     LGIDLVLPDFEYLRDRADDIEAIILTHGHEDHVGALPYALREIGFPKVIYGGMLTVGM
                     VRSKLDEHKLGSAPLTELPAGQKVSAGPFEIELVHLSHSIPDMRGVIIGTDLGKIFMT
                     GDYKFDQTPVDGRPADVARLAALGDEGLLLLCGDSTNADRPGVSPSESSVGPALYDTF
                     SRCKGRIIVTSFASNIHRVQQVIDAAERLDRKVALVGRSMRKNFNIASNLGMANAPEG
                     LLIQPREIEDFPDERVVVISTGSQGEPFSALRRMANNDHRDVELHSGDTVVFSATPVP
                     GNERAVNETIDRIYELGATVITPKDAPIHASGHGYREEIKMMLNLTRPKYVMPVHGDF
                     QRIRLHSELAESVGVPAENIFRNHNGQALLLGPDGAELTEETAGGVIYVDGVELSDPN
                     DATLRDRRTISADGIVIVVATIAEDDGEVVSDPEVIARGVPGLDEDAEFLDDLHDLIE
                     DSLDRAAEAKVRDHDLVQQDLHDAVAGFIHKRLKRRPMVVPVVVEV"
     gene            complement(5603..7093)
                     /locus_tag="PLJ64_07825"
     CDS             complement(5603..7093)
                     /locus_tag="PLJ64_07825"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNC93427.1"
                     /translation="MRRSRKWPPHWPIQWKLAAVSAGITFVILVAFGLAVGQITTNQL
                     RDNYAADTEAKANELAAEIRRRNLVAYLVTPGYIGDASGLSNLLASVNGAADVTTLAN
                     KTRFQLPNTHDLGDPSTTSGISTYDGWQVATAVVYQSEDIPTPVALVRFGRPMERLDA
                     SVGRIWISILAGALGATLLAALGGVILSRRAMRPISTLTSAAGQIARTRDPDVTLAEP
                     EGDDEVAELTRTFNDMLHELSIARVEREQSLKRQREFVADASHELRTPLTSVLANLEL
                     LDTSLRRPGSGNGDRDFEIESVESALRSSERMTRLVADLQLLAKADSERYRDLVPCDL
                     SEIAANVAAELRPLSDNHTIALDTPEAAVVEGNGDDLHRVILNLIDNAIRHTPAGSTI
                     TVTTRLDRDIAEIRVEDDGPGVPKEMWPTIFDRFVRHDGRGDRKKGKGTGLGLAIVRV
                     IARSHGGSARVGTSPEGGASFVVRIPAIKPAEKTPETASEKPQGNR"
     gene            complement(7128..7820)
                     /locus_tag="PLJ64_07830"
     CDS             complement(7128..7820)
                     /locus_tag="PLJ64_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC93428.1"
                     /translation="MVDEEQTGGRILVIEDDEEIVDVLRRTLRAEGYEVRATEDGGGG
                     LATASEFMPDLVVLDLGLPDMDGLEVCTRLREGSSELPILMLTARDGADNRVTGLDSG
                     ADDYLEKPYNREELLARIRALLRRHPPRGSAMLTVGDLKLNPDSQEAMRGDREVELTK
                     REFELLEYLMRNQRLVISRERLLEEVWGYDPLDETNTIDVFISNLRRKLEEGGEPRIL
                     HTKRGAGYVIKA"
     gene            7886..>8486
                     /locus_tag="PLJ64_07835"
     CDS             7886..>8486
                     /locus_tag="PLJ64_07835"
                     /inference="COORDINATES: protein motif:HMM:NF024882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HNC93429.1"
                     /translation="MFVRSVRRGSDRRPSRGASARGAKAEKPAREPLRQHHLDLIGLG
                     CVLLGIYLVFVLYFGWNGGRLGSGISDGLKYLFGLGAYLAPVALFAAGAIAIFRPAIP
                     KTRPLRTGAVLLAAGLLLAFAGGTFGIAGGRPERIAYFDPGWFPDHGGVIGESFYWAF
                     ASALQPFGAHLVAAFAILAGILLLTGTTVAAVLGRSGKAI"
BASE COUNT         1410 a         2772 c         2829 g         1475 t
ORIGIN      
        1 gcccctggcg ggcgagcaca atgtcctcaa cgccctcgcg gcgagcgcca tcgcgctggc
       61 cctggacgtg ccgctgccga cgctcaaggc cgggctggag ggtgccgaac acgtcaaggg
      121 ccgtctggtg ctctctggtg aatcgaccgc ggcccggatg tcgcggatcg cgcgcgccgt
      181 acttcacgac ctgcctctgc tcagtcttga cgagatgctg gccagggtcg atgctgtgag
      241 cgtggaggac gtttcagctt tggccagcga gctttacgcc gaggagcgac tttcggccgc
      301 atcggtaggt cgcgacgagg accgtttccg caaggcgatc cagccagtca gcgaggcgct
      361 cgcggcatga tccgggtggg agtcgccggg gccgccggcc gcatgggagc caccgtctgc
      421 ggcgcggtcg aggaagccga cgggctggaa ctgaccggcc gggccgatcc ggcgctggac
      481 accggactgt cggcgatcct gccggacgcc gacgtggtgg tcgagttcac gaccccggac
      541 acttcgcttg cgaattcgat cgcctgtctc gaggccggcg ttcacgtggt ggtcggggca
      601 accggctttg atctcgacgc gctccgggac gccgccgaga attccggcac tggcgccaac
      661 tgcttcgtcg ccccgaattt cgcgatcggt gccgtgctgc tgatggaact cgcccagaag
      721 gcggcaaggc acatgcccga atgcgagatc atcgagctgc atcacgatcg caagctcgac
      781 gcgccgtcgg gcacggcgaa gcggaccgag gaactgatca cggcggccgg cggcaacgtc
      841 caccagccga tccactcggt caggttgccg ggtctggtcg cccaccagga agtccttttc
      901 ggcggaacgg ggcaaacgct ctcgatccgt cacgacagca tcgaccgctc ctcgttcatg
      961 cccggagtgg tgctcgcggt acgcaaggta ggtcaactcg aagaccggtt cacggtcgga
     1021 ctggagaaga ttctggatga ctgatgtcgt agctcttgac gcaaccgcgc aggccgagct
     1081 ggtcgccaag ggagaggttt cgccggccga actggtcgcc ggcgcgatcg agcgggcgga
     1141 gaaggtcaat ccggaactga acgcgatctg ctcgccgctt ttcgagcagg cgaccgaagc
     1201 ggcggccggc gagctgcccg acgggccctt caggggggtt ccgttcctgt tcaaggatct
     1261 cggcgccggg ctggccggcc agccgatgca catgggcaac cgggtactga aggaaatcgg
     1321 catgaccatg ccctacgaca cgcacctcgg atcccgtttt cgcgatgccg gcctcgtcac
     1381 gctgggccgg accgctacgc cggagcttgg catcctgccg accacggaac cggtcctcaa
     1441 cgacgggccg accaagaacc cctgggacac gacgcgctct tccggcggtt cctcgggcgg
     1501 ctccgcggcg gcggtcgcgg cgggcatcgt gccgattgcc cacgcttccg acggcggcgg
     1561 gtcgatccgc atcccggcca gcgaatgcgg tcttgttggt ctcaaggcaa cccgggcccg
     1621 gatctcgcag gccccggtca tgggggactc gatgtccggc ctgaccaccg aactggcggt
     1681 tgcgcactcc gtgcgtgaca tcgccacgat gctggactgg gtccacgggc ccgcgccggg
     1741 tgacccctac ggctgcccgg tcccggcccg gccctactcg gaagaggtaa ccgccggtac
     1801 cgagccgctc aagatcgcgc tcctgaccga gtcgctcacc ggtgacagcc tgcagccggc
     1861 cgtggtcaac gcggcaaggg aaacggcgcg aaagctcgag gacctcggcc atgaagtgac
     1921 cgaattcgat ctgcccgcgg cgggcgagcc ggacgcgctc taccagacct tcgtgacccg
     1981 ctgggccgcc ggcatgagtc agaccgccga cacgctcagc ctgatcatcg gccggccgct
     2041 gaccagcgag gatgtcgaac ctctgacctg ggccctgatc gagaagggcc ggaccgaggg
     2101 cggggcggcg tacctttccg cggtcgccca gcatcaactg atcggtcgcc tcgtggccgg
     2161 ggtgatgctg gacggtcagt tcgacctgat catgaccccg accctggcca cggtgccgcc
     2221 caagctcggt catttcgatc agaacggacc cgacccgatg gatgcgatgc tgaaggcccg
     2281 tgagatcgcc accttcaccg gcatcttcaa cgcgaccggc cagccggcga tttcggtgcc
     2341 gatggagatg agcgaggaag gtctgccgat cgggatccag ttcgtggcgc cgatgtgggg
     2401 cgagcacgtg ctgatccagc tcgccggcga gctcgagcgg gcccatccct ggatcggccg
     2461 ccgggcgccg ggagttctcg acgccggata gggtgccttt tgtgagtgag atccgaggtg
     2521 taataacggc attggccacc gctttccggc ccgacggcga gctcgatatc gactcgaccc
     2581 ggaagctggc cgcctatctc gtcgagaacg gcagccacgg cctggtcgtg gccggttcaa
     2641 ccggcgagtc gtcaaccctg gaagacgacg agaagatcga tctcacgaaa gcggtagtgg
     2701 aagaggtcgg gccggaggtg acggtgatcg tcggttccgg ttcgaatgac acccgtcact
     2761 cggtcgagct gaccaagatg gcggcggaag ccggcgccga cgccgcgctg atcgtgacgc
     2821 cgtactacgt gcggccgaac cgggcaggaa ttcgccggca cttcgaagcc gtcgctgaag
     2881 gcagccccgg ctttccgatc gtcgcttaca acatcccttc ccgcaccggg atcaacatgc
     2941 ctcccgaatt ccttgccgaa ctggcgcaga tcgacgaagt caaggcggtc aaacaggcca
     3001 atgacgacga tctcgggccg atcgaggggc tcgacgtgct ggccggcaac gacggcacct
     3061 ttcttccgac actgaagttt ggcggggcgg gcggcatcct cgttgcctca cacctcgaca
     3121 acggccgcat gcgaacgatc tgggatctct ggcaggaggg ccgtcacgac gaggccgagg
     3181 ccctcgacaa ccagcttcag cccctttacg aggcgatggg cgtcgtcgtc aacccgattc
     3241 cggtcaaggc cgcgctcgcc gcccgcggcc tgcttcctcc gaccatgcgc ctgccgctgg
     3301 tcgagccgac cgatcaggaa cgtgacgccg tcgaagagct cctcgccgag gccggtgtgc
     3361 ccgccgcatg agccaggacc ggatcaagct gcttcccctc ggcggactgg gggaaatcgg
     3421 caagaacatg atggtgatcg agtaccgcgg ctcgatcgtg gtggtcgaca ccggtctgat
     3481 gttcccgacc ccggagatgc tcggcatcga cctcgtcctg ccggatttcg aatacctgcg
     3541 cgaccgggcc gatgacatcg aggcgatcat cctcactcac ggccacgagg accatgtcgg
     3601 cgcgctcccg tacgcgctgc gggagatcgg ctttcccaag gtcatctacg gcggcatgct
     3661 cacggtggga atggtccgtt cgaaactgga cgagcacaag cttggcagcg ccccgctgac
     3721 cgaactgccg gccggccaga aagtcagcgc cgggcccttc gagattgaac tggttcacct
     3781 ctcgcattcg atccccgaca tgcgcggggt gatcatcggc accgatctcg gcaagatctt
     3841 catgaccggc gactacaagt tcgaccagac cccggtcgat ggccgcccgg ccgacgtggc
     3901 ccgccttgcg gcgcttggag acgaaggcct gctcctgctc tgcggcgatt cgaccaacgc
     3961 cgaccggccc ggcgtatcgc cttctgaatc cagtgtcggc ccggccctct atgacacctt
     4021 ctcgcgctgc aaggggcgga tcatcgtcac ctcgttcgcg tccaacatcc accgcgttca
     4081 gcaggtgatt gatgccgccg agcggctcga ccgcaaagtc gccctggttg gccgttcgat
     4141 gcgcaagaac ttcaacatcg cctccaatct cggaatggcg aacgcccccg aggggctgct
     4201 gatccagccg cgggagatcg aggattttcc cgacgagcgg gttgtggtga tctcgaccgg
     4261 ctcccagggc gagcccttct cggccctgcg gcgcatggcc aacaacgacc accgggacgt
     4321 ggaactccac tcgggggaca cggtcgtctt ctccgcgacc ccggtgcccg gcaacgaacg
     4381 ggccgtgaac gagacgatcg accgcatcta cgaactcgga gcgaccgtga tcacgcccaa
     4441 ggacgccccg atccacgctt ccggccacgg ataccgggaa gagatcaaga tgatgctgaa
     4501 cctgacccgg ccgaagtacg tgatgccggt tcacggtgac ttccagcgga tccgcctcca
     4561 ctcggagctg gccgaatcgg ttggcgtgcc ggccgagaac atcttccgca accacaacgg
     4621 ccaggccttg ctgctcggcc cggacggcgc agagctgacc gaggaaaccg ccggcggcgt
     4681 gatctacgtc gacggcgtcg aactttccga ccccaacgac gccaccctgc gcgaccgccg
     4741 cacgatctcc gccgacggca tcgtgattgt ggtcgccacg atcgccgagg acgacggcga
     4801 ggtcgtttcc gacccggaag tgatcgcccg cggcgtcccc gggctcgacg aggacgcgga
     4861 gttcctcgac gacctccacg acctgatcga ggattcactc gaccgcgctg cggaagcgaa
     4921 ggtaagggac cacgacctgg tgcagcagga tctccacgac gccgtcgccg gcttcatcca
     4981 caagcgcctc aagcgccgcc cgatggtggt accggtagta gtcgaagtct gaggcccgca
     5041 cggcgcctgg agcgcggcgc cgggaggtgc cgcagaaaaa gccccgtcac ggggctgaac
     5101 gtgcggcacc ttcctgctct ctatggagcc gaatgaagcg tggcctgaac ccgccgctta
     5161 aaacagagag accgctgggc ctgggggagg gagtggctca acggtctgct ctgaagaagc
     5221 gaggttgctg gatgtctggg ggagaatgcc caacaacctc ttgtgtctcg tccaaaagca
     5281 acgatacgga cgcccagtaa gaaatcccta aacccgaagg aaggatcttc ttaaggttcg
     5341 tggacctcga ccagagtccg atcatccatc cttttttcgt gatcggacgg gttgaactgg
     5401 gagggcgggt cggacccccg cccgacttgc agtcaagccg ttaaacaaca agaccgccgg
     5461 gcctggggga gggaggtggc tcggcggtct gtgttgaaga agcgaagccg ccggggcagt
     5521 ctgggggaga tccccggcgg cttcatgtgt ctcgtccgcg aagaaagata cggagaggtc
     5581 ataaataggc cctaaagctt cgttaacggt ttccttgagg tttttcggag gctgtttctg
     5641 gggttttttc ggccggtttg atggccggaa tcctgaccac gaatgaggct ccgccctcgg
     5701 gtgaagtgcc gacccgggcc gaaccgccgt ggcttcgggc gatcaccctg acgattgcca
     5761 gtccgagtcc ggtgcctttc cccttcttgc ggtcgccgcg tccgtcatgc cgtacgaagc
     5821 ggtcgaatat cgtcggccac atttccttcg ggacgcccgg accgtcgtcc tcgacccgga
     5881 tctcggcgat atcgcggtcg agcctggtcg tcaccgtgat cgtgctcccg gcgggggtat
     5941 gccggatcgc gttgtcgatc aggttgagga tgacccggtg gaggtcgtca ccgttgcctt
     6001 cgaccaccgc cgcctccggc gtgtcgagcg cgatcgtgtg gttatcgctc agcggcctta
     6061 gctcggctgc cacgttggcg gcgatctccg aaaggtcgca gggcaccagg tcccggtatc
     6121 tctccgagtc ggccttggcg agcagctgga ggtcggctac gagccgggtc attcgctctg
     6181 atgagcgcag cgccgattcg accgattcga tctcgaaatc ccggtcgccg ttgcctgaac
     6241 ccggccggcg gagcgaagtg tcgagcagct ccaggttggc gagcacgctg gtcaggggag
     6301 tgcgaagctc gtgtgaggcg tcggcgacga attcgcgttg ccgcttcagc gattgctcgc
     6361 gttcgacccg ggcgatcgaa agctcgtgaa gcatgtcgtt gaaggtccgg gtgagctcgg
     6421 ccacctcgtc gtcgccttcc ggctcggcca gggtcacgtc ggggtcgcgg gtgcgggcga
     6481 tctggccggc cgcggaggtc agcgtggaga tcgggcgcat cgcccggcgc gagaggatca
     6541 cgccgccgag cgccgccagc agcgtggcac ccagggcacc ggccaggatc gatatccaga
     6601 tcctcccgac cgacgcgtcg aggcgctcca tcggccggcc gaaacggacc agggcgaccg
     6661 gggtcggaat gtcctcggac tgatagacga cggcggtggc aacctgccag ccgtcgtagg
     6721 tggagatccc cgaagtggtc gaagggtcac cgagatcatg cgtgttcggc agctggaagc
     6781 gggtcttgtt tgccagcgtc gtcacgtcgg ccgctccgtt caccgaggcg agcaggttgc
     6841 tgagcccgct ggcatcgccg atgtagccgg gggtgaccag gtaggcgacc aggttccggc
     6901 gccggatctc ggcggccagt tcattcgcct tggcttcggt gtcggcggcg tagttgtcgc
     6961 gcagctggtt ggtggtgatc tgcccgaccg cgaggccgaa ggcgaccagg atgacaaagg
     7021 tgattccggc cgagacggca gccagtttcc attggatcgg ccaatggggc ggccacttgc
     7081 gcgaccgtcg cattcgatcc aggagggtgc cttcggcccg ggccatctca ggccttgatt
     7141 acgtagccgg ctccgcgctt ggtgtgaagg atgcggggtt ccccgccttc ctcgagcttg
     7201 cgacggaggt tggagatgaa gacgtcaatc gtgttcgtct catcgagcgg gtcgtatccc
     7261 cagacctctt ccagcagacg ttctcgcgag atgaccaggc gctggttgcg catcaggtac
     7321 tcgagcagtt cgaactcgcg tttggtcagc tcgacttccc ggtcaccgcg catcgcttcc
     7381 tgggaatccg ggttcagctt caggtcgccg accgtgagca tcgccgagcc acggggcgga
     7441 tgtcgcctca gcagtgcccg gatgcgggcc agcagctctt cccggttgta gggcttctcg
     7501 agatagtcgt cggcgccgct gtctaacccg gtgacccggt tgtcggcgcc gtcccgggcg
     7561 gtgagcatca ggatcggcag ctcgctgctg ccttccctga gccgggtgca gacctcgagc
     7621 ccgtccatgt ccggcaggcc aaggtccagc acgaccaggt ccggcatgaa ttcggaagcg
     7681 gtcgcaaggc cgccgccacc gtcctcggtc gcccggactt cgtatccctc ggctctcagc
     7741 gtgcgcctga ggacatcgac gatttcctcg tcgtcctcga tgacgagtat gcgcccgccg
     7801 gtctgctcct catccaccat ggccgggatt ctaagcgagg tcgattacag gaagggtaaa
     7861 cgcgcgtttc gttgaaagga aagccatgtt tgtacgttca gtccggcgcg gttcagaccg
     7921 gcgcccgtcg cgtggcgcct cggcgcgcgg agccaaagcc gagaagcccg ctcgtgagcc
     7981 cctgcgccag catcacctgg acctgatcgg actgggctgt gtgctgctcg ggatctacct
     8041 cgttttcgtt ctctacttcg gctggaacgg cggacggctc ggttcgggaa tcagcgacgg
     8101 gctgaagtac ctcttcggtc tcggcgcgta cctggctccg gtcgcgctct ttgccgccgg
     8161 agcgatcgcg atcttccgcc cggccatccc gaagacccgt ccgctcagga ccggggcagt
     8221 gctgcttgct gcgggccttc tgcttgcctt cgccgggggt accttcggga tcgccggtgg
     8281 ccgtcccgag cggatcgcct actttgaccc gggctggttc ccggaccacg gcggggtcat
     8341 cggcgagagc ttctactggg ctttcgccag tgcgctccag cccttcggtg cccatctggt
     8401 ggcggcgttc gcgatcctcg ccgggattct gctgctgacc gggaccacgg ttgcggccgt
     8461 tctcgggcga agcggaaagg cgatca
//
LOCUS       DAOQKN010000103         8483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_4019_length_8483_cov_5.415876, whole genome shotgun sequence.
ACCESSION   DAOQKN010000103 DAOQKN010000000
VERSION     DAOQKN010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 8483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8483
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4019_length_8483_cov_5.415876"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..1597
                     /locus_tag="PLJ64_07840"
     CDS             92..1597
                     /locus_tag="PLJ64_07840"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNC93430.1"
                     /translation="MSRDHRKPSRYNQQFFRQWGGPGPLFFGLLALIIVLLGLYLAFT
                     KSIPFTSPGYQVKATFHNAVNIAIKSPVRIAGVNVGKVTDMEQKGDNTVVTFSVNDDG
                     RPIREDASAQIRPRLFLEGNWFIDLDPGTPDSSEMPDEGMIPVSHTGTSVQVGDLLRT
                     LQTPERANIQRLLESLGTGLNSKPTAEQDLTFEPFVQGLSGGEALNLAYRQGEVAARG
                     TAIVNAATLGTGPNDLGNLLRGLARVTGAVNERGDDLKGFVTNFATFTGALAAESENL
                     GLTIQKLGPTLVVARRALTSLNASLPALRGFAIASRPGLNELPRTIRVTRPFLRQLQQ
                     LLTEPELAGLAKVIQRSTPSAARSAAATLKLLPQLRNFGLCIADTVSPTGEQVISDGA
                     FSNGQKAARELFYAAVSTTGSSNGLDGNGSFARIQGGGGPISVKMSNPQESSANANML
                     YGRLQSAPLGTTPLAPGTPPIVSDQLCSEQELPDLNGPMAGPGSPSPVAYP"
     gene            1594..2907
                     /locus_tag="PLJ64_07845"
     CDS             1594..2907
                     /locus_tag="PLJ64_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNC93431.1"
                     /translation="MKRAIKTHFKDFLAITGLIVLALAVLLFILSNQKAALPSWIPWL
                     GQDFYSINVEMETAQAVTPGQGQAAVIAGINVGKVGASTLDNGMAKVRLDIEPEYKDL
                     IHPDAQFLLRPKTGLSDMVVEIDPGTKGPTPPEGTTFPVSQSMSNVQMDQILASLDRD
                     TQDYLVLLLNGAGEGLHDKGPELSQALRRFGPFATYTARLNGELEKRRKYIAKGVHSF
                     SEVAGALGDNADVVADFISASKENLGNYAAQEAALREALREFPATLDAGKVNLAKSDR
                     LSQLTRPTLLGLIPGARNLKSSLQSVQRLAIDTTPLVRDDIRPFTREVQPVFKELAKT
                     SQAGEKTTPKLQATFTQLNTLLNTLAYNPPGDSEGFLFYAPWASHNFNASVAFQDGLG
                     LVRRSVNMLTCSTSQSAAAVFGANVQLETAYRLNQLPPPTAICTP"
     gene            2934..4469
                     /locus_tag="PLJ64_07850"
     CDS             2934..4469
                     /locus_tag="PLJ64_07850"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNC93432.1"
                     /translation="MIAIAGFALSCFALLLFVWVAFGGPTPLAARGYEVKLPLTQVGQ
                     LAEQSEVDISGVEIGRVKSIDLGEGDEEGSAIVTLNLEPEYAPIPKDTRAILRAKSLL
                     GEAYIELTPGDKRDGMLDDGGELPPAQVAKSVQLDEIFRTFDEKTRQAFMQGAIDNSI
                     AISGRGATLNQTLGILPGTVTSLTDVLQVLNEQDQDVSKLIKNTGVVFDALSKRQGEL
                     SGMIRNTDTVFKTLADRDQELKDFFRVFPTFLRESRATQERLGDFSDFATPIVRKLVP
                     VAKQLSPTFKASANLAPVSERLYTNLKPVIRKAPRAFPSLRAFLDEDAPTLLGRLPDY
                     LAEFNPFLETGSLYRKEIAGLIGNAASATQPSISGGAHYVRAGVRLGPETYTSYPGTG
                     PSNGRVNPYTAPGGNLDFSSSGGMKVFSSANCTGSVNATIVPWSGLTPVQQAQFGANL
                     RYGFSAAQQEDWYTRAITYLMAGQNQTNNVPASACNQQAPFTPIGDESQPPTQYLHVF
                     REP"
     gene            4712..5818
                     /locus_tag="PLJ64_07855"
     CDS             4712..5818
                     /locus_tag="PLJ64_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93433.1"
                     /translation="MKKLVAIVVALIASLSMAASAQAANETVEGTTNISAINGPLYQT
                     KAVPVNLMIRAEVTTPAESPKVNPLKNSKITFPAGVSFNPNNSKTPACTDNMLSVTSN
                     LADPSGVVASCEDSVVGTGTSAIVLAKVNLPETLIEDPILVLFNAGTDSKGQAKVKIY
                     GYSKTTNVGILMHGTLVKGVLDVAVPVLSDDSAVKYYQFDLPGPGLDRDDINVHTEGK
                     DPNYVHGLCPASGVLKTNSEFILGERSYPSGTDTTPETTVDSPETTQNCTGQVGAPKL
                     SASVTGPKSVKSGAKGTFKVKVTNKGTGVAKSVSVTGTGGAKGSTKNINPDTAKTVTV
                     KAKVTGRKGSKKTLTFTAKSGKSSAKATIKVTVK"
     gene            5994..7037
                     /locus_tag="PLJ64_07860"
     CDS             5994..7037
                     /locus_tag="PLJ64_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93434.1"
                     /translation="MEATPGPGGLYREVARPIGASLTVTVTVPSESPKVTPIKVANVT
                     FPEDMDFFPNKEKTPPCPDSALNSQSNLAAGIAAIVDLCPKSVIGTGSSVVKLAKNNK
                     DSFANLTDPRLVIFNAGRNGKGRPKIVIYGYSKSVNAGLLMHGTLASDGQLRIEIGVM
                     PFDSAVSEFTLGIPGEDLEVPDDSAPGGSRTVKGLDRAYLRGKCSTGTWLATGKFVLG
                     ERSDPSGVPVGEETLLSSNPFELPCEGKAGHAKLAIRAIKGPRKIKARGKAGYVLTIS
                     NPGTATATNLRLGATGAVTGTGKTGSLGPGESSKVRLPVVAGSRKGKGKIRFRVSANG
                     SVRKESVRKILVG"
     gene            7262..7927
                     /locus_tag="PLJ64_07865"
     CDS             7262..7927
                     /locus_tag="PLJ64_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93435.1"
                     /translation="MQLSATVNTPASSLKVNPLKRAIIQFPKGLTFHPNNRKTPVCSD
                     KALSETSNLAAGPAGIVELCPKSVIGTGTAKIYLAKVHEDSALVSDPQLVIFNAGVAK
                     GGLAKMKIYAYSRTTNVGILMHGTVSKKGIINVFIPVLSNDSATASFILAIPGPGIKD
                     GGKTIKGRDANYARIRCASGKWVTRGTFTLGERSYPAGTPTGPSTTVKAKPSTVKCHG
                     ARG"
     gene            8056..>8483
                     /locus_tag="PLJ64_07870"
     CDS             8056..>8483
                     /locus_tag="PLJ64_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93436.1"
                     /translation="MGGDSLNGRGHMSHSKKVAGTFSALLVLALPLAGTAFAASETIT
                     LTTTTTPLSGKLFKEVRVPVNSSLSVEVHTPDDAPKVNPLKRSVMKFPTDLTYNPNNK
                     VTPVCTDSQLSDQSNLAAGVAGVVGLCPDSVVGTGTAAIYL"
BASE COUNT         1678 a         2835 c         2525 g         1445 t
ORIGIN      
        1 gggtcaacgc gcttcaggtc ggaaacctcg acggcgatca aggtctggtc actcaggacc
       61 agacccaggc tcagctgaac tggcggacca aatgagtcgc gaccaccgca agccaagccg
      121 ctacaaccag cagttcttcc gccagtgggg tggtcccggc ccgcttttct tcggcctgct
      181 cgcgctgatc atcgtgctgc tcggcctgta tctcgccttc accaagtcga ttccgttcac
      241 aagccccggc taccaggtca aggcgacctt ccacaacgcg gtgaacatcg cgatcaagtc
      301 cccggtccgc atcgccgggg tgaacgtcgg caaggtgacc gacatggaac agaagggtga
      361 caacaccgtg gtcaccttct ccgtcaacga tgatggccgg ccgatccgcg aggacgcttc
      421 ggcccagatc cgtccccgtc tcttcctcga aggtaactgg ttcatcgacc tcgatcccgg
      481 caccccggat tcatcggaaa tgccggacga gggcatgatc ccggtcagcc acacgggcac
      541 cagcgtccag gtcggcgacc tgttgcggac cctccagact cccgagcgcg ccaacatcca
      601 gcgcctgctg gagagcctcg gcaccggcct caactcgaag ccgaccgccg agcaggacct
      661 gaccttcgag cccttcgtcc agggcctgag cggcggtgaa gcgctgaacc ttgcctaccg
      721 ccaaggtgaa gtggctgccc gcggcacagc gatcgtcaac gctgccaccc ttggcaccgg
      781 cccgaatgac ctcggcaacc tgttgcgcgg cctggcccgg gtcactggtg cggtcaacga
      841 acggggcgat gacctcaagg gcttcgtgac caacttcgcg accttcaccg gcgccctcgc
      901 ggccgagtcc gagaacctcg gcctgaccat ccagaagctc ggcccgaccc tggttgtggc
      961 ccgccgcgcc ctgaccagcc tcaacgccag cctgccggcc ctgcgtggat tcgcgatcgc
     1021 ttcgcgtccc ggtctgaacg agctgccgcg gaccattcgg gtgaccaggc ccttcctccg
     1081 tcagctccag caacttctga cggaacccga attggccggc ctcgcgaagg tgattcagcg
     1141 ctcgactccg tccgcggcgc gctcggctgc ggcaacgctg aagctgttgc cgcagctgcg
     1201 caatttcgga ctctgcatcg ccgacacggt cagcccgacc ggcgaacagg taattagcga
     1261 tggcgccttc agcaatggtc agaaggcggc ccgggaactg ttctacgcgg cggtcagtac
     1321 gaccggcagc tcgaacggac tcgacggcaa cggctcgttc gcccggattc agggtggcgg
     1381 tggaccgatc tcggtcaaga tgtccaatcc gcaggaatcc tcggcgaacg cgaacatgct
     1441 ctacggccgc ctgcagtcgg ccccgctggg cactacgccg ctggcgcccg gcactccgcc
     1501 tatcgtgagc gaccaactct gcagcgagca ggaactcccc gacctcaacg ggccgatggc
     1561 cggcccggga tccccaagtc cggtggctta cccgtgaagc gcgcgataaa gacccacttc
     1621 aaggatttcc ttgcgatcac cggattgatc gtgctggcgc ttgccgtgct gctcttcatc
     1681 ctttccaacc agaaagcggc gctgccgtcc tggatcccct ggctgggcca ggacttctac
     1741 tcgatcaacg tcgagatgga gaccgctcag gcggtgaccc cgggccaggg ccaggccgcc
     1801 gtgatcgccg gcatcaacgt cggcaaggtg ggtgcctcga ccctcgacaa cggcatggcg
     1861 aaagttcggc tcgacatcga gcccgagtac aaggacctga ttcatccgga cgcccagttc
     1921 ctgctcaggc ccaagaccgg tctgagcgac atggtggttg agatcgaccc cggcaccaag
     1981 ggtccgactc cgcccgaagg gaccaccttc ccggtttcgc agagcatgtc caacgtccag
     2041 atggaccaga tcctcgcttc gctggaccgc gacacccagg actacctggt gctgctcttg
     2101 aacggcgccg gcgaggggct gcatgacaag ggccccgagc tttcccaggc gctccgccgc
     2161 ttcggtccct tcgcgactta cacggcccgt ctcaacggcg agctcgagaa gcgccgcaag
     2221 tacatcgcca agggcgtcca ctctttcagc gaggtggccg gggcactcgg tgacaacgcc
     2281 gatgtggttg ccgacttcat ctcagcctcg aaggaaaacc tgggcaacta cgccgcccag
     2341 gaagccgccc tgcgggaggc gctccgcgag ttcccggcga cccttgacgc cggcaaggtc
     2401 aacctggcca agtccgaccg cctttcgcag ctgaccaggc cgaccctgct cgggttgatc
     2461 cccggcgccc ggaacctcaa gtcctcgctc cagtcggtcc agcgcctggc aatcgacacg
     2521 actccgctgg tccgggatga catccggccc ttcacccgcg aggtccagcc ggtcttcaag
     2581 gagctggcca agacgagcca ggcaggggag aagaccaccc cgaagctgca ggccaccttc
     2641 acccagctga acacgctgct caacacgctc gcctacaacc cgcccggtga ctccgagggc
     2701 ttcctctttt acgcgccctg ggccagccac aacttcaacg catcggtggc cttccaggac
     2761 ggcctcggcc tggttcgccg cagcgtcaac atgttgacct gttcgacctc ccagtcggct
     2821 gcggccgtct tcggggccaa cgtccagctt gagactgcct accgactgaa ccagttgccg
     2881 cccccgaccg caatctgcac cccatgaaca aacaggcccc ttcagtcgga cagatgatcg
     2941 ccatcgcggg ctttgcgctc tcgtgtttcg cgctgctcct tttcgtctgg gtggccttcg
     3001 gcggcccgac acctctggcg gcacggggct atgaagtcaa gctgccgctg acccaggtcg
     3061 gccagctggc cgagcagtcc gaggtcgaca tctccggagt cgagattggc cgggtcaagt
     3121 cgatcgacct cggtgaaggc gatgaagagg gctcggcgat tgtgaccctg aacctggagc
     3181 cggaatacgc gccgattccg aaggacacaa gggcgatcct gcgggccaag agtctgctcg
     3241 gcgaggccta cattgaattg accccaggcg acaagcggga cggcatgctc gatgacggtg
     3301 gcgaactgcc gccggcccag gtcgcaaagt cggtgcagct cgacgagatt ttccgcacat
     3361 ttgacgagaa gaccaggcag gcattcatgc agggagcgat cgacaactcg atcgcgatca
     3421 gcgggcgcgg cgcgaccctg aaccagacgc tcggcatcct gccgggcacg gtgacctcgc
     3481 tgaccgacgt gctccaggtc ctgaacgaac aggaccagga tgtgtcgaag ctgatcaaga
     3541 acaccggcgt ggtcttcgat gcgctgagca aacggcaagg cgagctcagc ggcatgattc
     3601 gcaacaccga caccgtcttc aagaccctgg ccgaccggga ccaggaattg aaggacttct
     3661 tccgggtctt cccgaccttc ctgcgcgagt cccgggccac ccaggagcgg ctcggcgact
     3721 tctcagattt tgccaccccg atcgtccgca aactggtacc tgtcgccaag cagctttcgc
     3781 ctaccttcaa ggcctcggcc aacctggctc cggtcagtga acggctctac acgaacctga
     3841 agccggtgat ccgcaaggcg ccgcgggcgt tcccgtcgct aagggccttc ctcgacgagg
     3901 atgccccgac gctgctcggc aggctgcccg actacctggc cgagttcaat ccgttccttg
     3961 aaaccggcag tctctaccgc aaggaaatcg cgggactgat cggcaacgca gcatcggcaa
     4021 cccagccctc catttccggc ggtgcgcact acgtccgggc cggcgtccgt cttgggccgg
     4081 agacctacac ctcttacccg gggaccggcc catcgaacgg ccgggtcaac ccgtacacgg
     4141 ccccgggcgg gaaccttgac ttttcctcca gcggtggcat gaaggtcttc tcttccgcca
     4201 actgcaccgg ctcggtcaac gcgaccatcg tgccctggag cggccttacc ccggtccagc
     4261 aggcccagtt cggggccaac ctgaggtacg gcttcagtgc cgcccagcaa gaggactggt
     4321 acacgcgggc catcacctat ctgatggccg gccagaacca gaccaacaac gtgccggcat
     4381 ccgcctgcaa ccagcaggct ccgttcacgc cgatcggcga cgagtcccag ccgccgaccc
     4441 agtacctgca cgttttccgc gagccgtaaa tccgggcagg gtttttactt caggccaggc
     4501 aattggccgg attccgctct tggtccggat ttggctccct aagcgacaaa ttggcgatcc
     4561 gttgcatcat cattgcgacc gatctccaaa agcaccgcaa ctcagacgcc atattcgggt
     4621 tcgtgttcat gtaagctgcg accggttagg gagtgggagc ggctgacaag tcgcaaccac
     4681 gtgtaatcca caggagagaa gagagaccca gatgaagaag cttgtagcca tagtcgtggc
     4741 gcttattgcg tcactttcga tggcggcctc cgcccaggcg gcgaacgaga ccgtcgaagg
     4801 caccaccaac atcagcgcga tcaatggtcc gctgtaccag accaaggcag tgccggtgaa
     4861 cctgatgatc cgtgccgaag tcaccactcc ggccgaatca cccaaggtga acccgctgaa
     4921 gaactcgaag atcaccttcc ccgcgggcgt gagcttcaac ccgaacaaca gcaagacccc
     4981 ggcctgcacc gacaacatgc tcagcgtcac gtcgaacctg gccgatccgt cgggtgtcgt
     5041 cgcgtcctgc gaggactccg ttgtcggcac cggtacctcg gcgattgtcc ttgccaaggt
     5101 caacctgccg gagaccctga tcgaggaccc gatcctggtc ctcttcaacg ccggcactga
     5161 cagcaagggc caggccaagg tcaagatcta cggttactcg aagaccacca acgtcggcat
     5221 cctgatgcac ggcacgctgg tcaagggtgt tctcgacgtt gccgtccccg ttctctcgga
     5281 cgactcggcc gtcaagtact accagttcga cctcccgggc ccgggcctgg accgcgatga
     5341 catcaatgtc cacaccgagg gcaaggaccc gaactacgtc cacggcctct gcccggcgtc
     5401 gggtgtcctg aagacgaact ccgagttcat cctcggcgag cgctcgtacc cgagcggcac
     5461 cgacaccact cccgagacca ccgtcgactc acctgagacc acgcagaact gcaccggtca
     5521 ggtcggcgct ccgaagctct ccgcttcggt cactggtccg aagtcggtca agagtggagc
     5581 caagggcacg ttcaaggtca aggtcaccaa caagggcact ggtgttgcca agtcggtcag
     5641 cgtgaccggc accggcggcg ccaagggctc gaccaagaac atcaaccctg acaccgccaa
     5701 gaccgtcacg gtcaaggcga aggtcaccgg tcgcaagggc agcaagaaga cgctgacctt
     5761 cacggccaag agcggtaagt cctccgccaa ggccaccatc aaggtgaccg tcaagtagga
     5821 ctctcctact ggcagaagtt cgcaagaagg gcggccctcc ggggccgccc ttttgctttc
     5881 ctctcgcgat gggtttaaag atgagaaagc tgatctatcc cctggttctg gcagcggcct
     5941 ggctggggat ggcggccgcg gctgacgctg ccaacgagag catcggactc tcgatggagg
     6001 ccactcccgg tccgggaggg ctgtaccgtg aagtcgcccg accgatcggg gccagtctca
     6061 cggtcactgt cacggtgccg tcagagagcc cgaaggtgac acccatcaag gtggccaacg
     6121 tgaccttccc cgaggacatg gacttcttcc cgaataagga gaagaccccg ccctgtcccg
     6181 acagcgccct gaactctcag agcaacctgg ccgctggcat cgctgcgatt gtcgatctct
     6241 gtccgaaatc ggtcattggc accggttcct ctgtggtcaa gctggccaag aacaacaaag
     6301 attcgttcgc caacctcact gacccccgtc tggtcatctt caacgccggt cggaacggca
     6361 aaggccgacc gaagatcgtc atctacggct attcaaagtc ggtcaacgcc ggactgctga
     6421 tgcacggcac cctggccagc gatggccagc tcagaatcga gatcggcgta atgccgtttg
     6481 actctgcggt cagcgaattc acgcttggta ttcccggcga agacctggag gttcctgatg
     6541 attctgcccc cggaggaagc aggaccgtca aagggcttga tcgggcttac ctgaggggca
     6601 agtgttcaac cggcacctgg ctggcaaccg gaaagtttgt gctgggagag aggtccgatc
     6661 cgagcggcgt gccggtcggc gaagaaacgc tgctctcttc caaccccttc gagcttccct
     6721 gtgagggaaa ggccgggcac gcgaagctgg cgataagggc gatcaaagga ccgaggaaga
     6781 tcaaagcgcg cggcaaagcg ggatatgtcc tcacgattag caacccgggc acggccaccg
     6841 caaccaacct gcggctcggc gccaccggag cggtcaccgg aaccgggaag acaggctcac
     6901 tcggaccagg agagtcgagc aaagtccgac ttcctgtggt ggcgggatcc cgcaagggca
     6961 aagggaaaat ccggttccgg gtatctgcga acggctcagt tcgtaaagaa agtgttagga
     7021 aaattctcgt cgggtagata ggagcatcga aggaaatgcc ctcgtgggcg tttccgctta
     7081 aggaggctct gtgtttaacc tgaagcgaat cgtcgggctg gtctgtctgg cttgcgtgcc
     7141 ggcacttgtg ggtgtcgtgg gcgctggtcc cgcgtcggcc gcagacgaaa cgatcaacct
     7201 gaggaccgtc gtggtacctg cacacgggaa agtattcaag cgccgcagcg tgccggcaaa
     7261 aatgcagctc tcggcaacgg tcaacacccc ggcttcttcg ctcaaggtca atccgctgaa
     7321 gcgggcgatc attcagtttc ccaaggggct gaccttccat ccgaacaacc ggaagactcc
     7381 ggtctgttcc gacaaggccc tcagcgagac ctcaaacctt gccgccgggc cagccggaat
     7441 tgtcgagctc tgcccgaagt cggtgatcgg caccggtacc gccaagatct acctggccaa
     7501 ggtgcacgag gattcagcgc tggtctcgga tccgcagctg gttatcttca atgccggcgt
     7561 tgccaagggc ggcctggcca agatgaagat ctacgcctac tcgaggacca ccaatgtcgg
     7621 catcctgatg cacggcacgg taagcaagaa gggcatcatc aacgtcttca tcccggtgct
     7681 cagcaacgac tcggcgactg ccagcttcat tctggccatc ccgggccccg gaatcaagga
     7741 tggtggcaag accatcaagg gtcgtgacgc caactacgcc cggatccgtt gtgcatctgg
     7801 caagtgggtg acccgaggta ccttcaccct gggtgagcgg tcctacccgg cgggcacccc
     7861 gaccggcccc tcgaccacgg tcaaggccaa gccttcaacc gtcaagtgtc acggcgctcg
     7921 aggctagggc agttgagcct ctgaggaggc agatcaattg ctgaaacggg ccggggactg
     7981 gcaacagtct ccggcccgtt tgtcgttcgc tgccaatcat cccccggcag gctgtaaagt
     8041 ccaagatgag tcgtcatggg aggcgattca ctgaacggga ggggtcacat gtcgcactcg
     8101 aagaaagtgg cgggaacatt cagcgccctg ctggttctgg cgctgccttt ggccggcacc
     8161 gccttcgcgg caagcgagac gatcaccctg accacgacca cgaccccgct ctccggcaag
     8221 cttttcaagg aggttcgggt accggtcaat tcctcgctct cggtcgaggt ccacaccccg
     8281 gatgatgccc cgaaggtgaa cccgctgaag cggtcggtga tgaagttccc gaccgatctg
     8341 acctacaacc cgaacaacaa ggtcaccccg gtctgcacgg acagccagtt gagcgaccag
     8401 tccaacctgg cggctggagt ggccggggta gtcggccttt gccccgattc ggtggtcggg
     8461 accgggacgg cggcgatcta cct
//
LOCUS       DAOQKN010000104         8469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_4035_length_8469_cov_5.506537, whole genome shotgun sequence.
ACCESSION   DAOQKN010000104 DAOQKN010000000
VERSION     DAOQKN010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 8469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8469
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4035_length_8469_cov_5.506537"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="PLJ64_07875"
     CDS             <1..889
                     /locus_tag="PLJ64_07875"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNC93437.1"
                     /translation="TPELAIGFMDAHGIARQVLSVSDPGVDFLAGEAAVKLARECNDY
                     LAGVVASHPDRFSGLAVVSGNDPEAALSEIARCLERPGFAGLGLLSSYDGSYLGDPAF
                     DPLLTELDQRHAWVMVHPTAVPTTDKPDLTIPDFMAEYPFDTTRAFLNLLVHNAFARF
                     PGIRWHFAHGGGTIPMLAARMDVAAAHGKVLGPLLGLPEQSADLGPESAREALAGCWY
                     DTALIASEGGLQAVSAMTGTDHILFGCDWPFAGLAYPPEGDPQPAIGTLFGPDDQEAI
                     YRANAEKLLAGLPQQTESA"
     gene            complement(982..1587)
                     /locus_tag="PLJ64_07880"
     CDS             complement(982..1587)
                     /locus_tag="PLJ64_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93438.1"
                     /translation="MSNLVTRIKQFRPATLIAMLALFVAIGGTATAASGLINGKKIKN
                     NTVTGKKLKNRTITKNKLAPATVKALKGQQGPKGDTGQPGKDGVVSPTYQEFGSTNII
                     DGNELAIGTVNVPAGKYMVTATANTFALGSGRTECFVTTNNGGGSSETSSWNSTAANV
                     RNALPVVYVTDTDTVTSITLGCGIDDTNGSVGGSMIVTPVQ"
     gene            complement(1712..2110)
                     /locus_tag="PLJ64_07885"
     CDS             complement(1712..2110)
                     /locus_tag="PLJ64_07885"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNC93439.1"
                     /translation="MGERSRIGSGAVWEERFGYSRAVKAGHLIFVSGTTAADPTGGPP
                     IGGDDIAAQARECFRRIEVALGELGSGLGEVVRTRMFVTDIGLSEAVGLVHGEIFGRQ
                     APAATMVEVSALISPDLLVEIEVDAVSSSD"
     gene            2156..2521
                     /locus_tag="PLJ64_07890"
     CDS             2156..2521
                     /locus_tag="PLJ64_07890"
                     /inference="COORDINATES: protein motif:HMM:NF014687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HNC93440.1"
                     /translation="MPTSEENDKARTNFAAARTTLAGERTQLAWVRTGLASIAVGIGL
                     ARVVPLLDSGAIAWPYEALGVAYCAFGLGLIFFGVQRGKLSAEHHVEKPTVPAGGTFL
                     AAGGLILGIATILVVALAR"
     gene            complement(2538..3446)
                     /locus_tag="PLJ64_07895"
     CDS             complement(2538..3446)
                     /locus_tag="PLJ64_07895"
                     /inference="COORDINATES: protein motif:HMM:NF006128.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HNC93441.1"
                     /translation="MKLETMVCETGEGQGHIVLNRPERGNGLTPELIRELAETVERLD
                     LDPEVRVIVLSGNGSGFCGGYDLVASAEKMLEGEFGSEDAPAGSPLDPMVQAANHDPS
                     KTWDPMVDYAGMSRNVRAFMSLFHCTKPVICKVHGFCVAGGTDLALCSDLLVIADDAK
                     IGYPPARVWGSPTTSLWAYRLGAQRAKRLLFTGDSLTGREAAEAGLAIESHPREKLDE
                     AVDALAARIAMVPLNQLQMMKLLVNQSLYAQGLHQTQLIGTVFDGITRHTPEGFAFQQ
                     RAAEAGFKQAVRERDEPFGDFGLKPD"
     gene            3506..5209
                     /locus_tag="PLJ64_07900"
     CDS             3506..5209
                     /locus_tag="PLJ64_07900"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HNC93442.1"
                     /translation="MELSLVAVGGVVSVVAVALLSRRVGIAAPLLLVLAGAAIGALPR
                     VSATDIEPQWILGGVLPPLLYSAAINMPTTDFRRNLKPITGLAVLLVVVSTGIAGVLF
                     HTLMPEIGWPAAFALAAVLSPTDAVAATAVGRRLGLPSRLLVILEGEGLVNDVTALVL
                     LRSAVAAIGAGVTVDGVIGDFIVAVVGAIGIGLLVGIVNVRIRQRISDPVMNTAISFV
                     VPFLAYIPAEDFGFSGIFAVVVCGLVTSDLAARYLRAEDRLTEETNWQTLAFLMEGFI
                     FLLMGISLEDVLSYAGNNGFSIEQAVLYGLIVAVLLIVVRVMFVVPLVAMIKRDDQRA
                     ADAKPRVEDFKQKLERDELPDDMLRHGTERVEHRIAAVSADIEFRISQSLGWRAGAVL
                     AWSGMRGAVTVAAAQTLPLDTPYRAELVLIAYVGAAAMLLLQGSTLPWVINRFRVQGD
                     DPERIRNEYGDMINEISAAGLEELDRQAEGGRFSPEMIERVKRDSLIREGGARRSSED
                     PDRDRQQYIDLRLAVLGAERDRMLHHRRVGTYSAEVIDRTQRILDLEEARLQQVSGEP
                     R"
     gene            complement(5273..6745)
                     /locus_tag="PLJ64_07905"
     CDS             complement(5273..6745)
                     /locus_tag="PLJ64_07905"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HNC93443.1"
                     /translation="MAELKQLTSLDAQFLAIETDRNYGHVGGLAILDPTTAPGGKLTI
                     EDLRQLIQDRLHLIAPFTQRLVSVPLSIDWPYWTQDEHFDIGYHCREIALPEPGSHEQ
                     LLEQVGRIYSRRLDRSRPLWEIYLIHGLEGGKVALLSKTHHAAIDGMSGAEILSALYD
                     LGPEPRKVDPPATAPDRSAPDGKDLLMMWVKGIPRSPLNLVDRLTTVVPHVDMAVAAF
                     GLPGSRRISRVMSRVRIRFGAEEEHVIERPPVKAPKGPYGAALSPHRVFGLGQVSLDD
                     VKTVKNAFGVKVNDVVICLVAGAVREHLTFTGELPEEPLLAMIPVSVRTEEERGTFGN
                     KVSAMIVPLPTHIDDPAERLEFIHSVMGAAKDSHDALPAEALRDLTDFVPPALHARAA
                     RVVTTVAGRFTRPPWNVIISNVPGPPIDLYFAGAKLEAMYPLSIISDGIGLNVTIMSY
                     RNSIDIGITADREQTPEVQTLVDGMQSELELLLAAARAAA"
     gene            complement(6747..7496)
                     /locus_tag="PLJ64_07910"
     CDS             complement(6747..7496)
                     /locus_tag="PLJ64_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93444.1"
                     /translation="MNDGANWLADTATAVADRLGLPHNRLFDEWASTLDPSRWPEPPE
                     GDGRPAMLIPGFLAGDPSLSNMARWLKRGDWNMTRSGINWNVNCTQATIEGVEARLRA
                     SVEASGKRALLVGQSRGGAMGKVIAVRNPDLVETLVTLGSPLNDHFNIHPRTWFGIGI
                     IGGLGELGFPGFLSRDCATGDCCKETRIAYGRPFPKDVRFISFYSRADTIVRYQACLH
                     QAAEHVEIESSHLGMGLDSEVWVRMSEELSR"
     gene            complement(7626..>8469)
                     /locus_tag="PLJ64_07915"
     CDS             complement(7626..>8469)
                     /locus_tag="PLJ64_07915"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HNC93445.1"
                     /translation="PAANNGCVSLKPTTGRVPDEGHVPTVPPPVNAWNHAGPLARRVE
                     DLALALGVLDGSGEADLPGVEGIRCRVFTGNRSMIVSPEVKSAVRRAAGALGSAGMEV
                     EQSPALPIQRMTMLYSHVLRKHALPEINAELGGGERFNWLAEVRRTLTGRARISAEAL
                     SVYAYIAYGGVLKPGPGDADRKLERLRRQVIETVGDGVLVCPLLLVRPARHGATWLPF
                     TQIPMAVPFNITGLPVAMVPVYWTANGLPLSVQVVAGPGKDELALAAARELEERLGGW
                     KPID"
BASE COUNT         1339 a         2780 c         2799 g         1551 t
ORIGIN      
        1 gacccccgaa cttgcgatcg gcttcatgga cgcccacgga atcgcgcgac aggtcctttc
       61 ggtttccgat cctggcgtcg acttcctcgc cggcgaggcg gcggtgaagc tggcccgcga
      121 atgcaacgac tacctcgccg gcgtggtcgc ctcgcatccc gatcgcttca gcggcctggc
      181 agtcgtatcc ggaaatgatc cggaagcggc gctttccgaa atcgcccggt gcctcgagag
      241 acccggattt gcaggcctcg ggctgctctc gagctacgac ggcagctatc tcggtgatcc
      301 cgcctttgac ccgctcctga ccgaactgga ccagcggcat gcctgggtca tggtccaccc
      361 gaccgctgtc cccaccacgg acaaacccga cctgaccatc cccgacttca tggccgagta
      421 cccgttcgac accacgcggg ccttcctcaa ccttctggtc cacaacgcgt ttgcccgctt
      481 tcccggcatt cgctggcact ttgcccatgg cggaggcacc atcccgatgc tcgccgcccg
      541 catggacgtg gccgcagctc atggcaaggt gctcggcccg ctcctcgggc tgccggagca
      601 gtcagccgat ctcggtcccg aaagcgcccg ggaggcgctc gccggatgct ggtatgacac
      661 ggcgttgatc gcttcggaag gcggacttca ggcggttagc gcgatgaccg gcaccgatca
      721 cattctcttc ggctgcgact ggccctttgc cggtctcgcc tatccgcccg agggtgaccc
      781 ccagccggcg atcggcaccc tgttcggccc ggacgatcaa gaggcgatct accgggccaa
      841 tgcggaaaag ctgctggccg gccttccgca gcagaccgaa tcagcctagg gcgcgagggc
      901 cagggcgacg acgatgcacg aacgccgccc ccgttcccgg gggcggcgcg tgtagctcta
      961 tggcttccag ccgaaggttc gttactggac cggggtgacg atcatcgaac cgccgaccga
     1021 gccgttggtg tcatcgatgc cgcagccaag ggtgatcgag gtgaccgtgt cggtgtcggt
     1081 gacgtaaacg accggcaggg cgttgcggac gttcgcggca gtcgagttcc agctcgaggt
     1141 ttcgctgctg ccgccgccgt tgttggtcgt gacgaagcat tcggtccggc cgctgccgag
     1201 ggcgaaggta ttcgccgttg ccgtgaccat gtacttgccg gccgggacgt tgacggtccc
     1261 gatcgcgagc tcgtttccgt cgatgatgtt ggtgctgccg aactcctggt aggtcgggct
     1321 gacgacgccg tccttgccgg gctggccggt atcgcccttc gggccctgct gccccttgag
     1381 ggccttgacc gtggccggag cgagcttgtt cttggtgatc gtcctgttct tcagtttctt
     1441 gccggtgacc gtgttgttct tgatcttctt gccgttgatc aggccggaag ccgcggttgc
     1501 ggtgccgccg atggcgacga agagggccag catggcgatc agggtggccg ggcgaaactg
     1561 cttgatgcgg gtgacgaggt tgctcattgt tgcctttcgt tgtgcttcgg ttgattggcg
     1621 cttctgattg gtcaactggc gagcagccaa ttacttacgc ggatttcgaa aatcttttcc
     1681 ggaaccccga tatttcgctt agcagagcgg ttcagtccga ggaagaaact gcgtcaacct
     1741 cgatctccac gagcaggtcg gggctgatca gggccgagac ctcgaccatg gtcgccgccg
     1801 gtgcctgcct gccgaagatc tctccgtgga ccagcccgac cgcttcgctc aggccgatgt
     1861 cagtcacaaa catgcgggtt cgaacgactt cgccgaggcc cgagccgagc tccccgagcg
     1921 cgacttcgat ccggcgaaag cactcccggg cctgggcggc gatgtcatcg cctccgatcg
     1981 ggggtcctcc cgtcggatcc gccgcggtgg ttcccgagac gaagatcagg tgcccggcct
     2041 tgacggcccg cgaatagccg aatctttctt cccagacggc gccggagccg attctcgatc
     2101 tctcccccat gtccggttca gggtatccgc gcggtggccg gattaccatt gcgtcatgcc
     2161 cacctccgaa gagaacgaca aggcccggac aaacttcgcc gccgcacgca cgacgctggc
     2221 cggtgagcga acccagctgg catgggtgcg gacgggactc gcctcgatcg cggtcggcat
     2281 cggattggcg agagtcgtcc cgctactcga ttccggagcg atcgcctggc cctatgaagc
     2341 cctgggcgtt gcctactgcg ccttcggcct gggcctgatc ttcttcggcg tccagcgcgg
     2401 caagctctcg gccgaacacc atgtcgagaa gccgaccgtg ccggctggcg gaacgttcct
     2461 cgccgcggga ggactcatcc tcgggatcgc aacgatcctc gtggtcgcgc tcgcccgctg
     2521 agccggacca gcccggctca gtccggtttc aggccgaagt ccccgaatgg ttcgtcgcgt
     2581 tcgcgaaccg cctgtttgaa gccggcttcg gccgcccttt gctggaaggc aaagccctcc
     2641 ggggtgtggc gggtgatgcc gtcaaagacc gtgccgatca actgggtctg gtggaggccc
     2701 tgggcgtaga gcgactggtt gacgaggagc ttcatcatct gcagctggtt caggggaacc
     2761 attgcgatcc gggcggccag cgcgtcgact gcctcgtcga gcttctcacg cgggtgcgac
     2821 tcgattgcca gaccggcttc tgccgcttcc cggccggtaa ggctgtcacc ggtgaagagc
     2881 agccgcttgg cccgttgcgc cccgagcctg taagcccaga gcgaagtggt cggagaaccc
     2941 cagacccggg ccggcgggta gccgatcttg gcgtcgtcgg cgatcaccag aaggtcgctg
     3001 cacagggcca ggtcggtgcc accggcgacg cagaagccgt gaaccttgca gatcaccggt
     3061 ttggtgcagt gaaacagcga catgaaggcc cgcacgttcc ggctcatgcc ggcgtagtcg
     3121 accattggat cccaggtctt cgacgggtca tggttggccg cctgcaccat cggatcgagc
     3181 ggtgagccgg cgggcgcgtc ctcggagccg aattcacctt ccagcatctt ctcggcgctt
     3241 gccacaaggt cgtatccccc gcagaacccg ctgccgttgc cggagagaac gattacccgg
     3301 acctcggggt cgaggtcgag acgctccacc gtttccgcca gttcgcggat cagctctggc
     3361 gtcagtccat tgccgcgctc cggacggttc aggacgatgt gtccctgacc ctcccctgtc
     3421 tcgcagacca tcgtctccag cttcatgagg cgatgctaac cgggccttga gccggaggtt
     3481 agtttcgcgg ttaggcttcg ctgccgtgga gctctccctg gtcgcagtcg gcggtgtcgt
     3541 cagcgtggtc gcggtagcac tgctttcccg ccgcgtcggg atcgccgcac cgcttctgct
     3601 ggtgctggcc ggagccgcca tcggagccct cccccgggtt tcggcgaccg acatcgagcc
     3661 gcagtggatc ctcggcggag tgctgcctcc gttgctctac tcggcggcga tcaacatgcc
     3721 gaccacggac ttccggcgga acctgaaacc gatcaccggg ttggccgtgt tgctggtcgt
     3781 cgtctcgacc gggatcgccg gggttctgtt ccacaccctg atgccggaaa tcggctggcc
     3841 ggcagctttc gccctggccg cggtcctgag cccgaccgat gcggtggccg ccacggcggt
     3901 cggccggcgc ctcggcctgc cctcccggct tctggtcatc ctcgaaggcg aggggctggt
     3961 caatgacgta accgccctgg ttctgctgcg cagcgccgtc gccgcgatcg gcgccggggt
     4021 gacggttgac ggtgtgattg gagacttcat cgtggccgtg gtcggtgcga tcggcatcgg
     4081 cctgctggtc gggatcgtca acgtgcggat ccggcaacgg atctcggacc cggtgatgaa
     4141 tacggcgatc tccttcgtcg tccccttcct cgcttacatc ccggccgagg acttcggctt
     4201 ttcgggcatc ttcgcggtgg tggtctgcgg cctcgtgacc agcgacctgg ccgcgcggta
     4261 cctgcgggcg gaagaccgtt tgactgaaga gaccaactgg cagacgctgg ccttcctgat
     4321 ggagggcttc atcttcctgc tgatgggcat cagcctcgag gacgttctct cctatgcggg
     4381 caacaacggg ttcagcattg agcaggccgt tctctacggg ttgatcgtcg cggtgctttt
     4441 gatcgtggtc cgggtcatgt tcgtcgtgcc cctggtggcg atgatcaaac gagacgacca
     4501 acgggccgcg gacgcaaaac cacgggttga ggacttcaaa cagaagcttg aacgagacga
     4561 actgccggac gacatgctcc ggcatggaac cgaaagggtc gagcaccgga tcgccgccgt
     4621 ctcggccgac atcgagttcc gcatctctca gtcgctcggc tggcgggccg gtgccgttct
     4681 cgcctggtcg ggcatgcgcg gcgcggtgac cgtagcggcc gcccagaccc tgccgctcga
     4741 caccccgtac cgggccgaac tggtcctgat cgcctatgtc ggcgcggcgg cgatgctgct
     4801 gctccagggt tcgaccttgc cctgggtgat caaccgcttc cgggtccagg gcgatgatcc
     4861 cgaacggatc cgcaacgagt acggggacat gatcaacgag atctccgccg ccggtcttga
     4921 ggagctcgat cggcaggccg agggcggccg gttcagcccc gagatgatcg aacgggtcaa
     4981 acgcgacagc ctgatccgtg agggcggcgc gaggcgttcc tcggaggatc ctgaccggga
     5041 ccggcagcag tacatcgacc tgcgtctggc agtgctcggg gccgagcgcg accggatgct
     5101 gcatcaccgg cgggtcggca cctacagcgc cgaggtgatc gacaggaccc agcgcatcct
     5161 cgacctcgag gaggcccggc tccagcaggt ctcgggcgag ccgcgctaac tccgttcctg
     5221 cccagcccgt ttcactcgtt gccccgcgcc atcggcccga acggggcggg tctcaggcgg
     5281 cggcgcgggc tgcggccagc agcagctcga gttccgactg catgccgtcg accagggtct
     5341 ggacctcggg ggtctgctca cggtcggcgg tgatcccgat gtcgatcgaa ttccggtagc
     5401 tcatgatcgt cacgttcaac ccgatcccgt ccgaaatgat cgagagcgga tacatggcct
     5461 cgagcttcgc cccggcgaag tagaggtcga tcgggggtcc ggggacattc gagatgatca
     5521 cgttccaggg gggccgggtg aagcgcccgg ccacggtggt gacgacccgg gcggcccggg
     5581 cgtgaagtgc cggcggcacg aagtcggtga ggtcacgcag ggcctcggcc ggaagggcat
     5641 cgtgactgtc cttggcggca cccatgaccg aatgaatgaa ctcgagccgc tcggcgggat
     5701 catcgatgtg ggttggcagc ggcacgatca tcgccgagac cttgttgccg aaggtgccgc
     5761 gctcttcctc ggtccggacc gagacgggga tcatcgccag cagcggttcc tccggcagct
     5821 cgcccgtgaa ggtgagatgc tcgcggaccg cccccgcgac caggcagatc accacgtcgt
     5881 tgaccttgac tccgaacgcg ttcttgacgg tcttcacgtc atcgagggaa acctgtccca
     5941 ggccgaaaac gcggtgcggc gaaagtgccg cgccataagg acccttcggc gcctttaccg
     6001 gcggcctttc gatcacatgc tcctcttcgg ctccgaagcg gatcctcacc cgggacatga
     6061 cccggcttat ccgccggctg cccgggaggc cgaaggccgc aaccgccatg tccacgtgcg
     6121 gcaccacggt ggtcagccgg tcaaccaggt tgagcggcga gcggggaatc cccttcaccc
     6181 acatcatcag caggtccttg ccgtccggag cggaccggtc cggagcggtc gccggcgggt
     6241 ccaccttccg cggctcggga ccgaggtcgt aaagggcgga gaggatttcg gcgccggaca
     6301 tgccgtcgat cgccgcgtga tgggtcttcg aaagcagcgc gaccttgccg ccctcgagcc
     6361 cgtggatcaa atagatctcc cagagtggac gggaacgatc gagtcgccgc gaatagatgc
     6421 ggccgacctg ctccagcagc tgctcgtgac tgcccggttc cgggagcgcg atctcccggc
     6481 agtggtaacc gatgtcgaag tgttcatcct gggtccagta cggccagtcg atgctcaacg
     6541 gaaccgagac cagccgctgg gtgaacggcg cgatcaggtg cagccggtcc tgaatcagct
     6601 gcctcagatc ttcgatcgtc agcttgccgc cgggagccgt ggtggggtcg agaatcgcga
     6661 ggccgccgac gtggccgtag ttccgatcgg tttcgatcgc cagaaactgg gcgtcaagtg
     6721 aggtgagctg cttcagctcg gccatctcag cggctgagtt cctcgctcat gcggacccag
     6781 acttccgagt cgaggcccat cccgaggtgg ctgctctcga tctcgacgtg ctcggcggcc
     6841 tgatggagac aggcctggta acgcacgatg gtgtcggcgc gcgagtaaaa ggagatgaag
     6901 cggacatcct tggggaacgg ccggccgtag gcgatccggg tttccttgca gcagtccccc
     6961 gtggcacagt cacggctgag gaaaccgggg aaaccgagtt caccgagtcc gccgatgatg
     7021 ccgatcccga accaggtgcg ggggtgaatg ttgaagtgat cgttcagcgg ggagcccagg
     7081 gtcaccaggg tctcgaccag gtcggggttg cggaccgcga tcaccttgcc catcgctccg
     7141 ccccggctct ggcccaccag caaggcccgt ttgccgctgg cttcgaccga cgcccggagt
     7201 cgcgcctcga cgccttcgat cgtggcctgg gtgcagttga cgttccagtt gatgccgctc
     7261 ctggtcatgt tccagtcgcc gcgtttcagc cagcgggcca tgttcgaaag gctcgggtca
     7321 ccggcgagga aaccggggat cagcatcgcc ggccggccgt caccctcggg aggttcgggc
     7381 caccgggatg gatcgagggt cgatgcccat tcgtcaaaaa gccggttgtg cggaagtccc
     7441 agccggtcgg cgaccgcggt ggcggtgtca gccagccagt ttgcgccgtc gttcacggac
     7501 ggcagcgttg gaacctttcc aattgatgga aaccctccca gtggcaattc catgtgatga
     7561 atgttactcc ccggcgcagc ggaccagcca tgtgttgcgc cccggaggcc gggttgcaga
     7621 ctggctcagt cgatcggttt ccagccgccg agccgctcct ccagctccct cgcggcagcc
     7681 agggccagtt cgtccttgcc cgggccggcc accacctgaa ccgaaagtgg aagaccgttc
     7741 gcggtccagt acaccggcac catcgccacc ggcaacccgg tgatgttgaa agggaccgcc
     7801 atcggaatct gggtgaatgg caaccaggtc gccccgtgtc gggccggtcg gaccagcaaa
     7861 agcggacaga cgagcacacc gtccccgacc gtctcgatca cctgccggcg cagccgttcc
     7921 agcttccggt cggcatcacc cggaccgggt ttgagaacgc cgccgtaggc gatgtatgcg
     7981 tagaccgaga gtgcctcggc cgagatcctt gcccggccgg tgagggtgcg tcgaacttcc
     8041 gcgagccagt tgaaccgctc gcctcccccg agttcggcgt tgatctcggg caatgcgtgc
     8101 tttctgagca catgcgagta gagcatggtc atcctctgga tcggcaaagc cgggctctgc
     8161 tcgacttcca ttccggccga tccgagtgcg ccagccgcac gccggaccgc ggatttcact
     8221 tcggggctga caatcatcga gcggttgccg gtgaagaccc ggcagcggat cccttccacc
     8281 ccgggcaggt ctgcttcgcc gctgccgtcg agcacaccca gcgcgagggc gaggtcttcg
     8341 acccggcggg ccagtggtcc cgcatggttc caggcattca ccggtggcgg cacggtcggc
     8401 acgtggcctt cgtcgggcac ccggccggtg gtcggcttca gggaaacgca gccgttgttc
     8461 gcggcgggg
//
LOCUS       DAOQKN010000105         8453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_4048_length_8453_cov_4.333413, whole genome shotgun sequence.
ACCESSION   DAOQKN010000105 DAOQKN010000000
VERSION     DAOQKN010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 8453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8453
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4048_length_8453_cov_4.333413"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..784)
                     /gene="hisA"
                     /locus_tag="PLJ64_07920"
     CDS             complement(59..784)
                     /gene="hisA"
                     /locus_tag="PLJ64_07920"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HNC93446.1"
                     /translation="MILYPAIDILGGKAVRLLKGEYDQETRYFDDPADAARQWADGGA
                     EYIHVVDLDGAKTGEPVNLGRIEKIAAAVNCPIEVGGGIRDLESAGRILDAGAARVVV
                     GTAAMRDREFLDALIAERGAEQVVVGIDARGGQVSLAGWTESSGVDVADAAAELNRRG
                     VVRFLFTPIEVDGTMEGPNIPELIRVAGATDASVIASGGVGNLTHLEDLARRAPENVE
                     GVIVGKALFEGKFTVEEGIAALK"
     gene            complement(781..1401)
                     /gene="hisH"
                     /locus_tag="PLJ64_07925"
     CDS             complement(781..1401)
                     /gene="hisH"
                     /locus_tag="PLJ64_07925"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HNC93447.1"
                     /translation="MAAEPKIAILDYGMGNLRSVEKAFQKVGANAVVTADETEIASAD
                     GLVLPGVGAFPRAMERIGELGLDRMLHDAVGAEKPVLGICLGLQLLFQSSIEQGGSEG
                     LGLLEGDVVQVPAGGRKVPHIGWAEVTWEKPDELTNGLRPGEPFYFVHSFVVQPTESE
                     LLGTASYGSRFACVAGKGKIWGVQFHPEKSSSAGLRMLTNFREICA"
     gene            complement(1423..2013)
                     /gene="hisB"
                     /locus_tag="PLJ64_07930"
     CDS             complement(1423..2013)
                     /gene="hisB"
                     /locus_tag="PLJ64_07930"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HNC93448.1"
                     /translation="MSRQAKIERKTKETGIELSLDLDGSGYRNETGVGFFDHMLDLLA
                     RHANLGLEVKATGDLETGSHHTVEDVGIVLGQAIDQALGDRAGIRRYGSAVVPMDEAC
                     AECALDISGRALSVFRGEIPVTSIANYETELTEEFFRAIAGGAKMTLHIDIRYGSNVH
                     HMIEAAFKAFARALRVAVSIDPDEKGVPSTKGTLNG"
     gene            complement(2010..3299)
                     /gene="hisD"
                     /locus_tag="PLJ64_07935"
     CDS             complement(2010..3299)
                     /gene="hisD"
                     /locus_tag="PLJ64_07935"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNC93449.1"
                     /translation="MISRRFEWAEPERLAGQIEDYLSHEARSVDVRGIAAEVAERGDA
                     ALLDLTAKFDAPEAESLNLRVGQDDALAALGNLAPQVRSALETAIANVRAVAAAQLGP
                     GEKAVTLPEGHTVTVGEVPVGAAGIYAPGGRASYPSTVVMGCVTARVAGVDRVVLVAP
                     PGPDGSIDPTTLAAAALCEVDEIYAVGGAQAIFALARGTETIRPVDVIAGPGNAWVQA
                     AKREVFGEVAIDSLAGPSDLTVVVDSATNLHWAALDLCAQAEHGEESPLVAIATEPGV
                     IERLEAEVDAVAADNPTVKDCHLALVEAPSAADAIELVNLIAPEHLELMSEGSAALAG
                     AVRTAGCVFTGEETGTAFGDYVAGSNHVLPTDGTGRIFGPLSPATFRRATARVSLDAE
                     SARKLAGPLDALARAEGLPVHGLSATARANDPDQETE"
     gene            complement(3296..3964)
                     /gene="hisG"
                     /locus_tag="PLJ64_07940"
     CDS             complement(3296..3964)
                     /gene="hisG"
                     /locus_tag="PLJ64_07940"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNC93450.1"
                     /translation="MFDELRKPGGKLKLAVPRGALLDEVLDLLEKVGIETAAVRGDSR
                     SLVFDGGELTVITMRPSDVPTYVSAGAADLGITGKDVLLEQGDRPVYELVDLGIGPCR
                     MVLATQKGDVAPSEHQRRLGMMKIATKYKRIAEEYFAEQGRQVELVEVKGSIELAPLV
                     GLADGIVDLVATGRTLADNNLEVREEILSSTGRLVANRVSYRIRREEVDGLAQRLREV
                     VGES"
     gene            complement(3957..4976)
                     /gene="hisZ"
                     /locus_tag="PLJ64_07945"
     CDS             complement(3957..4976)
                     /gene="hisZ"
                     /locus_tag="PLJ64_07945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HNC93451.1"
                     /translation="MIRPIPPGTRDVLPDEMRELRAIQSALLGTFDAAGFSEVRTPTL
                     EYADALELGDGRSADSAYRFFDELGEMLALRTDMTIPIARLVADRFRDLDPPHRLCYF
                     ANAYRAVTPQRGELREFGQAGVELIGVPGPEGTAEVVELLDRSLTAVGLDEAVIGLGE
                     DGLWTALLQDAGAGPETIELSSELLVSHDLVGVERALAESDELDPEAIGQAATLIQLR
                     GGAEVLEAARGLGGQGFAVVLERLEENFRAITDRVSSRSVLLDLGMLREIGYYSGSTI
                     EVYDETVGDVIGGGGRYDDLMNRFGLDLPAAGFTLYTERIHKAQLERRGVGPRAATGE
                     RDSDV"
     gene            complement(4989..6275)
                     /gene="murA"
                     /locus_tag="PLJ64_07950"
     CDS             complement(4989..6275)
                     /gene="murA"
                     /locus_tag="PLJ64_07950"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNC93452.1"
                     /translation="MQKFVIQGGVPLSGEITAAGNKNAALPILAACLLTEEVVTIANV
                     PRIRDTEAQLALLESIGVEVEWVEENTVRLCAREVENKALDEDLSAAIRASFLLAGPL
                     LARFGSVEMPPPGGDFIGRRRLDAHLDAFRDLGARVGGKRWIELQAPPSGLKAAKIFM
                     DEPSVMGTENALMAAALTEGTTVISNAASEPHVQDLALLLDQMGAKIEGIGSNVMTVH
                     GQKKLGGTEFRVSPDHIEIASFMALAAATGGKLRIKEVKASDLMMIRRQFRRLGLQSV
                     IEGTDLIVPPRQELMIQPDMGGAIPKIEDGPWPAFPADLTSIAVALATKAHGEILIFE
                     KMFENRLFFVDKLVAMGAKITLCDPHRVIISGPTDLHGERVDSPDIRAGMAMLIAALA
                     AEGKSEIGNIRQIDRGYENIDDRLRSLGAHIERVDE"
     gene            6366..7613
                     /locus_tag="PLJ64_07955"
     CDS             6366..7613
                     /locus_tag="PLJ64_07955"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNC93453.1"
                     /translation="MAQASTGKRRGVGSRIGWPLKIFVGILLVLLVLLILNAFALNNE
                     TKSASVNVPGAQLVETTAGKLQVLDTGTPSGSMSAGQSLPIVLIHGSAGAINWWDDLI
                     PLLKPYHRVIAIDLLGYGGSDKPDSDYSIETQANLVAQALGKLNISRAVLVGHSPGGS
                     VVTSVAEQSPDLVSGLVLIDSGPDSSYGGLSGAAKASRTPILGQALWRLAPDSMIKKN
                     VSQGFAPGYDVPDKYVQDVRNMTYSAYRKSSTGVEDYTDGKSLPDRLAELGKPLLVIF
                     GEEDQIYDAREALSAYAAVPGAKTHLILNSGHSPQIEAPAQTARAIENFTRTITRSES
                     AASGKAAVAQARKEAAESKKESGRKSNRQSDQAQQNAGNKSGQAKQKAAQLKRQASEK
                     ARKARQEALQAQKEAAGSKVESP"
     gene            7798..8328
                     /locus_tag="PLJ64_07960"
     CDS             7798..8328
                     /locus_tag="PLJ64_07960"
                     /inference="COORDINATES: protein motif:HMM:NF012344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93454.1"
                     /translation="MEVTFGGFQIAVLAAFLFVALSLVAIFAVVARQARRNVEFAEVT
                     DTGYRLRRYWFAFLCVLLGSGVVLSLFFLPYPDNAKSGATVNVSGGLFYWSMSPDTFK
                     AGSDVTFDVTSSDVNHGLGVYDPDGVLVGSVQAMPGYQNQLDLTLDEPGTYMLACLEY
                     CGYKHHEMMREFEVTP"
BASE COUNT         1409 a         2894 c         2675 g         1475 t
ORIGIN      
        1 cccttgacga cgcggccgtt gtccacgtcg aggcagggga tgattctctt gaggtcgctc
       61 atttcagcgc cgcgatcccc tcttcgaccg tgaacttgcc ttcgaacagg gccttgccga
      121 cgatcactcc ctcgacgttt tccggcgccc gtcgagccag atcctcgagg tgggtcaggt
      181 tgccgacccc gccggacgcg atcaccgaag cgtcggtcgc tcccgccacc cggatcagtt
      241 cggggatgtt cggaccttcc atggtgccat cgacctcgat cggggtgaac aggaacctga
      301 ccacaccacg gcggttcagc tcggccgccg cgtcggccac gtccaccccg ctcgactcgg
      361 tccagccggc cagcgagacc tgcccgcccc gggcatcgat cccgaccacg acctgctccg
      421 ccccgcgttc tgcgatcaag gcatcgagga actcgcggtc gcgcatcgcg gcggtgccga
      481 ccacgacccg ggcggctccg gcgtcaagaa tgcggccggc cgactcgagg tcgcgaatcc
      541 cgccgccaac ctctatcgga cagttgaccg ctgccgcgat cttttcgatc cggcccaggt
      601 tgaccggctc gccggtcttc gcgccgtcga gatcgaccac gtggatgtac tcggccccgc
      661 cgtcggccca ttgccgggcg gcatcggcag gatcgtcgaa atagcgggtc tcctggtcgt
      721 actcgccctt caggaggcga accgccttgc cacccagaat gtcgatcgcc gggtagagga
      781 tcacgcgcag atctccctga agttcgtgag catgcggaga ccggccgaac tcgacttctc
      841 gggatggaac tgaacccccc agatctttcc cttgccggcc acgcaggcga agcggctgcc
      901 gtaggaggcg gtgccgagaa gttcggattc ggtcggttgg acgacgaagg aatgcacgaa
      961 gtagaagggt tcgccgggcc tgagtccgtt ggtcagttcg tcgggctttt cccaggtcac
     1021 ctcggcccag ccgatgtgag gcaccttgcg gccgccggcc ggcacctgga ccacgtcacc
     1081 ctcaagcagc ccgagacctt cggacccgcc ctgctcgatt gaagactgga agagcaactg
     1141 caggccgaga cagataccga gcaccggctt ttcggccccg accgcatcgt gaagcatccg
     1201 gtccagccca agctcgccga tccgctccat cgccctggga aaggcgccga ccccgggcag
     1261 cacgaggcca tcggccgagg cgatctccgt ttcatccgcg gttacgaccg cgttcgcgcc
     1321 gaccttctgg aaggcctttt cgaccgaacg gaggttgccc atcccgtaat cgaggatggc
     1381 gattttcggc tcagcggcca cggccagacc ccttgtcgcg gctcaaccgt tcagcgtgcc
     1441 cttggtgctg ggcacgccct tctcatcagg gtcgatcgaa accgcgaccc gcagcgcgcg
     1501 ggcgaaggcc ttgaaggccg cttcgatcat gtgatgcacg ttcgagccgt aacggatgtc
     1561 gatgtggagg gtcatcttcg ccccgccggc gatcgcccgg aagaactcct cggtcagttc
     1621 ggtctcgtaa ttggcaatcg aggtgaccgg aatttccccc cggaaaaccg aaagggcccg
     1681 gccggagatg tccagcgcac attcggcgca agcctcgtcc atcggcacca cggcgctgcc
     1741 gtagcggcgg attcccgccc ggtcaccgag ggcctggtcg atcgcctggc cgagcacgat
     1801 gccgacgtct tcgaccgtat ggtgggagcc ggtttccaga tccccggtcg ccttgacctc
     1861 gagaccgagg ttggcgtggc gggcgagcag gtccagcatg tgatcgaaga aaccgacccc
     1921 ggtttcattc ctgtacccgg agccgtcgag atcgagactc agctcgatgc cggtctcctt
     1981 cgtcttccgt tcgattttcg cctgtctgct cattctgttt cctgatctgg atcgttcgcc
     2041 cgggccgtcg ccgagagccc gtggaccggc agcccctccg cccgggccag ggcgtccagc
     2101 gggccggcca gtttgcgggc cgattcggcg tccaagctta cccgcgccgt tgcccgtctg
     2161 aaggtggcgg gcgaaagcgg tccgaaaatc ctgccggtgc cgtcggtggg cagcacgtgg
     2221 ttggagccgg ccacgtaatc gccgaaggcg gtaccggtct cttcgccggt aaacacgcag
     2281 ccggcggtgc gaacggcccc ggccagggcg gccgagccct cgctcattag ctcgaggtgt
     2341 tcgggcgcga tcagattgac caactcgatc gcgtccgcgg ccgaaggcgc ttcgaccagg
     2401 gcgagatggc aatccttgac ggtcgggttg tccgccgcaa cggcgtcgac ctcggcttcc
     2461 aggcgctcga tcacacctgg ttcggtggct atcgcgacca gcgggctctc ctctccgtgc
     2521 tcggcctggg cacagagatc gagcgccgcc cagtgcaggt tggtcgccga atccaccacg
     2581 acggtcagat cggatggtcc ggccagcgag tcgatcgcga cctcgccgaa cacctcccgt
     2641 tttgccgcct ggacccaggc gttgcccggt ccggcgatca cgtcgaccgg gcggatcgtc
     2701 tcggttccgc gggcgagcgc gaagatcgcc tgggcgccgc cgaccgcgta gatctcgtcg
     2761 acttcgcaaa gtgccgccgc ggccagggtc gtcggatcaa tcgaaccgtc cggcccgggc
     2821 ggggcgacca gcacaacccg gtccaccccg gccacccgcg cggtcacgca gcccatgacc
     2881 accgtcgagg gataggacgc acgcccgccc ggggcataga tgcccgcggc cccgaccgga
     2941 acctcgccga ccgtgacggt gtggccttcc ggcagggtca ctgccttttc gccgggcccg
     3001 agctgagccg cagcgaccgc cctgacgttg gcgatcgccg tttccaaagc gctgcggact
     3061 tgcggggcga ggttccccaa cgcggccagg gcatcgtcct gaccaacccg gaggttcagc
     3121 gattccgctt ccggcgcgtc gaacttcgcg gtcagatcga gcagggcggc gtcgccgcgt
     3181 tctgccacct cggccgcgat cccccgcacg tcgacgctgc gggcttcatg gctgaggtaa
     3241 tcctcgatct gtccggccag ccgctcgggt tcggcccact cgaatcggcg ggaaatcatg
     3301 attctccgac aacttcgcgc agccgctgcg ccagcccgtc cacttcctcg cggcggatcc
     3361 ggtacgagac ccggttcgcg accaggcggc cggtgctgct caggatctcc tcccggacct
     3421 cgaggttgtt gtcggccagg gtgcgaccgg tcgcgaccag gtcgacgatg ccgtcggcca
     3481 ggccgactag cggagcgagt tcgatcgaac ccttgacttc gaccagttca acctgccgtc
     3541 cctgctcggc gaagtactcc tcggcaatcc gcttgtactt ggtagcgatc ttcatcatgc
     3601 ccagacggcg ctgatgctcg gaaggggcaa cgtcaccctt ctgggtcgcc agaaccatgc
     3661 ggcagggtcc gatcccgaga tcgaccagtt cgtagacggg ccggtcaccc tgttcgagca
     3721 gcacgtcctt gccggtgatg cccaggtcgg cggcgccggc ggaaacgtag gtgggcacgt
     3781 ccgacgggcg catcgtgatc acagtcagct cgcctccgtc gaagaccagc gagcgggaat
     3841 cgccgcgcac ggcggcggtc tcgatcccga ccttctcgag caggtcgagc acttcgtcca
     3901 gcaacgcgcc tctgggcacc gccagcttga gctttccgcc gggcttgcga agctcgtcaa
     3961 acatcactgt cccgttcccc ggttgccgct cgcgggccga cgccccttcg ctccagctgg
     4021 gccttgtgga tccgctccgt atagagggtg aatccggccg ccggcaaatc gagaccgaac
     4081 cggttcatca ggtcgtcgta gcggccgccg cctccgatca cgtcaccgac cgtctcgtca
     4141 taaacctcga tcgtcgagcc gctgtagtag ccgatctcgc gcagcatgcc cagatcgagc
     4201 agcacgctgc gactgctgac ccggtccgtg atcgccctga agttttcttc caggcgttcc
     4261 agcacaacgg caaaaccctg accgccgaga ccgcgggccg cttccaggac ttcggctccg
     4321 ccccgaagct ggatcagggt ggccgcctgt ccgattgctt ccgggtccag ttcgtcggat
     4381 tcggccaggg cccgttcgac accgaccagg tcgtgagaga ccagcagctc ggagctgagt
     4441 tcgatcgtct ccggccccgc cccggcgtcc tgcagcagcg cggtccagag tccgtcctcg
     4501 ccgagaccga tcacggcctc gtccagtcct actgccgtca gcgaacggtc gagcaactcg
     4561 accacctcgg ccgtgccctc cgggcccggc acaccgatca gttccacccc ggcctggccg
     4621 aactcacgca gctcgccccg ctgcggggtg accgcgcggt aggcgttggc gaagtagcag
     4681 aggcgatgcg gcgggtcgag atcgcggaag cggtcagcga ccaggcgggc gatcgggatc
     4741 gtcatgtccg tcctgagcgc cagcatctcg cccagttcgt cgaagaagcg gtaggccgaa
     4801 tcggccgaac ggccgtcgcc gagctccagg gcatccgcat attcgagggt cggggtccgg
     4861 acctcgctga atcccgccgc gtcaaaggtt ccaagcaggg ccgactggat ggcgcgaagt
     4921 tcgcgcatct cgtccgggag cacgtcccgg gttcctgggg ggatgggacg gatcaacctt
     4981 gtccttccct actcgtctac acgctcgata tgggcgccga gcgaacgcag ccgatcgtcg
     5041 atgttctcgt aaccgcggtc gatctggcgg atgtttccga tctccgactt gccttccgcg
     5101 gcgagcgccg cgatcagcat cgccatcccg gcccggatgt ccgggctgtc gaccctttcg
     5161 ccgtgcaggt cggtcgggcc gctgatgatc acccggtgcg ggtcgcaaag cgtgatcttg
     5221 gcccccatcg cgaccagctt gtcgacgaag aagagccggt tctcgaacat cttctcgaag
     5281 atcaggatct cgccgtgagc cttggtcgcc agggcgacgg cgatcgaggt caggtcggcc
     5341 gggaaggcgg gccaggggcc gtcttcgatc ttcggaattg ccccgcccat gtcgggctgg
     5401 atcatcagct cctgacgggg cggcacgatc aggtcggtgc cttcgatcac cgactgcagt
     5461 ccgaggcggc ggaactggcg gcggatcatc atcaggtcgg aagccttgac ttccttgatt
     5521 cgcagcttgc caccggttgc cgcggccagg gccatgaaac tggcaatttc gatgtgatcg
     5581 ggagacaccc ggaactcggt cccgcccagc ttcttctggc cgtgcacagt catcacgttc
     5641 gagccgatcc cctcgatctt cgcccccatc tgatcgagca acagggccag atcctgaacg
     5701 tggggctcgg aggcggcgtt gctgatcacg gtggtgccct cggtcagggc ggctgccatc
     5761 agggcgtttt cggtgcccat cacggagggc tcgtccatga agattttggc ggccttcagg
     5821 cccgaaggcg gcgcctggag ctcgatccag cgcttgccgc cgacccgggc tccgaggtcg
     5881 cggaaggcgt caaggtgggc atcgaggcgg cggcggccga tgaagtcgcc gccgggcggc
     5941 ggcatctcaa ccgagccaaa ccgggccagg agcggaccgg ccagaaggaa ggaagcgcgg
     6001 attgcggccg agaggtcttc gtccagggcc ttgttctcga cctccctggc gcagagccgg
     6061 accgtgttct cctcgaccca ctcgacctcg accccgatcg actcgagcag cgccagctgg
     6121 gcctcggtgt cccggatccg gggtacgttc gcaatcgtca cgacctcctc ggtgaggagg
     6181 catgcggcga ggatcggcag cgcggcgttc ttgttgcctg cggccgttat ctcgccggag
     6241 agcggaaccc cgccctgtat tacgaacttc tgcatctgtt tttgtacccc gcggtttgac
     6301 ggttttcgtg tgaccggacg gggacattgc agcccgaccg aagcgggtct agggtaacac
     6361 cggtcatggc acaagcttcc actggcaaga ggcggggcgt cggctcccgg atcggctggc
     6421 ccctcaagat cttcgtgggg atcctcctcg tgctgctggt cctgctcatt ttgaacgcat
     6481 tcgcgctcaa caacgagacc aaatcagcga gcgtgaacgt gcccggagcc caactggtcg
     6541 aaaccacggc cggcaagctc caggtgctgg atacgggcac accatccggg tcgatgtccg
     6601 ccggccagag cctgccgatc gtcctgattc acggttcggc cggggcgatc aactggtggg
     6661 atgacctgat tcccctgctc aagccgtatc accgggtcat cgcgatcgac ctgctcggct
     6721 acggcggctc ggacaaaccg gattccgact actcgatcga gacccaggcg aatctggtcg
     6781 cccaagccct gggcaagctc aacataagcc gggccgtgct ggtcggccat tcgcctggtg
     6841 gcagtgtggt cacctcggtc gccgagcagt cccccgacct tgtttccgga ctggtcctga
     6901 tcgacagtgg tcccgacagc tcctacggcg ggctttccgg agcggcgaag gcctcccgca
     6961 cgccgatact gggtcaggcc ctgtggcgac tcgcccccga ctcgatgatc aagaagaacg
     7021 tttcacaggg gttcgcgccc ggttacgacg tgcccgacaa gtacgtccag gacgtccgca
     7081 acatgaccta ctccgcgtac aggaaatcct caaccggagt cgaggattac accgacggga
     7141 agtcgctccc cgaccggctg gccgaactcg gcaaaccgtt gctggtcatc ttcggcgagg
     7201 aagaccagat ctacgacgcc cgcgaagccc tcagcgccta cgccgccgtt ccgggcgcaa
     7261 agacccacct gatcctgaac tccggccact cgccccagat cgaggccccg gcccagaccg
     7321 ccagggcaat cgagaacttc acccggacca tcaccaggag cgaatcggca gcgtccggga
     7381 aggcggcggt cgcccaggcc aggaaggaag ccgccgagtc gaagaaggaa tccggacgga
     7441 agtcaaaccg gcaatccgac caggcccaac agaacgccgg gaacaagtcc ggacaggcaa
     7501 agcagaaggc cgcgcagctc aagcggcagg ccagcgagaa ggcccggaag gcaaggcagg
     7561 aagccctgca ggcccagaag gaagcagccg ggtccaaggt cgagtcaccc tgaccgtccg
     7621 gctttcgccg gcgctcctcc ccgtttccgc ctacgccggc ctcgacggtc gtcaaccgct
     7681 gctttccggc cacgaagccg gaaatcatgc gggaaactac tgattttcgc tgcttttcag
     7741 ccggtaaggt tcgcgccgtt caagccggat ttccctgcct gagctaaagg agcgaacatg
     7801 gaagtcacgt tcgggggttt tcagattgca gtgcttgccg cgtttctttt cgtggccctg
     7861 tctctggtcg ccattttcgc ggtggtcgcc cggcaggccc ggcgaaacgt cgaattcgcg
     7921 gaggtcacgg acaccggata ccggcttcgc cgctactggt ttgccttcct ttgcgtgctc
     7981 ctcggctcgg gcgtagtcct ctccctcttc ttcctgccct atccggacaa cgccaagagc
     8041 ggcgcgaccg tcaacgtgag cgggggactc ttctactggt cgatgagtcc ggacaccttc
     8101 aaggccggca gcgatgtcac tttcgacgtt acctcgagcg acgtaaacca cgggctcggt
     8161 gtctatgacc ccgatggggt cctggtcggc agcgtccagg ccatgccggg ttaccagaac
     8221 cagcttgacc tgaccctcga cgagcccggc acctacatgc tggcctgcct cgaatactgc
     8281 ggctacaaac atcacgagat gatgcgggag ttcgaggtca cgccgtgaac gagatggccg
     8341 cggctgaagt gctccatcca ctcggagagc aactgagtcc gaggtcttcg aaaccccaga
     8401 atctcgtcgg ctggctcttc ggtggaaccg ggttggcgct gttcgcgctg atg
//
LOCUS       DAOQKN010000106         8353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_4130_length_8353_cov_6.169077, whole genome shotgun sequence.
ACCESSION   DAOQKN010000106 DAOQKN010000000
VERSION     DAOQKN010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 8353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8353
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4130_length_8353_cov_6.169077"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..1694
                     /locus_tag="PLJ64_07965"
     CDS             63..1694
                     /locus_tag="PLJ64_07965"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNC93455.1"
                     /translation="MGKKLVLAYVDSLRTDMLKQTIEESRAPTFRRLLKRGTLIEDCV
                     SSFPSVTPVASAEMVTGAQADRHWISGMNWFHRAEQRYVEYGSSLEATRAFGLFRSLH
                     DLVYNMNLSHLSPSVETVFETLDDADIRTASTPFLIYRGRHRHEVNIAGITGKAIEAG
                     LLKFDYGTWGPRELFYGDLYTSMETKCKPSSIPGKRDMYSACCGAELISRDAFDFMLL
                     AFPDNDNYSHRHGPLASVRSIERADYCMAEVIRAAGGVEPFLQDHALILLADHAHTAV
                     EHRYDIIEWMSRKWKVLPPSSEEPAEAELAVSPTSRAAHIYLLAEQDNPIRHRRVREH
                     LVGLEGTELVSWTERDGQPVVRSTRPGHLEPGNVAVVERDGRRLDFFPLSSGANGDGQ
                     GEVPQKQPRDGAKRAAPSPGEGRETVTDRRGRQWSISGNIEALGASVEEGRIETPEYP
                     DGLGRLWSALVAPHGGDIIVSAAPGYECVDWGGAAHVPGGSHGSLRKEDSEGPLLFVG
                     CGPDSPDPETAARHRSRPQWGLQDVAPIIREFFGT"
     gene            complement(1727..2527)
                     /locus_tag="PLJ64_07970"
     CDS             complement(1727..2527)
                     /locus_tag="PLJ64_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HNC93456.1"
                     /translation="MKLLFIGDVVGSPGRKGLASLMPRLREEHSPDLIVVNGENSAGG
                     LGITEKTAKEIFACGADVITLGNHTYRQRESWDYLDRAERVIRPANYRRANPGKGHCV
                     VQVNPADGSPARRVAVINLIGKIGLDAARSPFDEADLILNDLEKSGGADTVVVDFHAE
                     VTSEKVAMGWYLDGRVAAVFGTHTHVPTADGRVLPGGTAFISDVGMTGARDSVLGVKK
                     EQAIEKMRTDMPIRFESATDDVWVMGAAVEIDENGLATGFKQILEPSS"
     gene            2563..3303
                     /locus_tag="PLJ64_07975"
     CDS             2563..3303
                     /locus_tag="PLJ64_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC93457.1"
                     /translation="MQTPLTGTGPALAARGVSKSFDGRKALRGVDFEVGTGEVVALIG
                     PNGAGKTTLLSILAGIAEPDSGEVTGNGVAWVPQQAALYRRLGVRENLLLFARLQHDA
                     GQTGEAPEQMVTRMLELTGLGDRAGDQVGTLSGGNQQRVNIAIGLMAGPSVLLLDEPS
                     VGLDPAQRERLWGFVLGLVGEGTAVAFSTHDIQEAERYAERIVVVAEGEGIFSGTADA
                     LRQAVEVEGGPAGDLETSFLAYLRGRGH"
     gene            3303..4646
                     /locus_tag="PLJ64_07980"
     CDS             3303..4646
                     /locus_tag="PLJ64_07980"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC93458.1"
                     /translation="MRWLLLKDLQILKRSPLQLTLLILYPIVIALLVGFAMTRDQDQT
                     KVALYNAMPAGEPLGFGGEEVDGNEIRDQLCGRVDCVEADSTADAKKMVESGEATAAV
                     IVPEDLGDKIRSLASLNPESPEIQIIVNGSDAVESRVTDDRIEAMLGEANLLLAKRLS
                     EGAKEYLDLILQGGEFDLLGQQIEVLGLKRSEQILKQIEPKLPPGTDREQLGRAVRFA
                     HLATDNLDLAGPLLTAITEPIKAEKVEVGDDAPSLDTFAIGVTVAFALMFVTVLLVAG
                     SLALEKEENAFTRLTAGLVSRGQILVSKVGLGTVVGFVVALILLTGLGLFVGIEWGRA
                     PYWLLALIFGAAAFSAGGAALGAAAREVRAATLAAVMICLPVALLSLVPDDAVGPVIE
                     TAINLVSAIFPFRPTLDAITGGLEISGPTLWLPLLHLVILTAAYGALARLALRRF"
     gene            4762..5739
                     /gene="hemB"
                     /locus_tag="PLJ64_07985"
     CDS             4762..5739
                     /gene="hemB"
                     /locus_tag="PLJ64_07985"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HNC93459.1"
                     /translation="MSFPSTRLRRLRRTPALRGLVRETGLSAADLIQPLFVTAGTDLR
                     EPVESMPGVNRFSIAALVEEAGEIQAAGIPAVLVFGVPSEKDEAGSGAYDDEGVVQMA
                     VRALKESHPDLIVITDVCLCEYTDHGQCGFVRDGQVDNDVTVELLARTAISHAEAGAD
                     IVAPSDMMDGRIGSIRYQLDEEGHPDTAILSYAAKYASAFYGPFREAAESTPAFGDRR
                     GYQMDPANSDEAVREAKLDLEEGADALMVKPAGHYLDIVRRVKDETGAPVAAYQVSGE
                     YSAIRAAGANGWIDERAAALESLVSIRRAGADFIVTYFAREAAAWLAES"
     gene            5765..7192
                     /gene="hemL"
                     /locus_tag="PLJ64_07990"
     CDS             5765..7192
                     /gene="hemL"
                     /locus_tag="PLJ64_07990"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HNC93460.1"
                     /translation="MSGLSRGAPDKVTIKVRGDGSPLAETGSEVEYREEEKAKAAAAQ
                     AAAGRAGNEELFERARRVLPGGVNSPVRAMGQVGIDPVFIESARGAEVLDRQGRRYLD
                     WVSSWGPMILGHADPEVLAAIEEAAGRGTSFGAATELEVELAEEVAERMPSVEMIRMT
                     SSGTEAAMTAARLARAATGREVLVKFAGAYHGHSDGLLAEAGSGVATHGIPSGPGIPE
                     AQAAGTVVIPWNDREAVAEALEKHKVAALFAEPVAANMGVVPPEEGYLEFLRQATARA
                     GAMLVFDEVITGFRVARGGAQELFGIVPDLTVMGKIIGGGLPAAAVGGSRELMERLAP
                     TGDVYQAGTLSGNPLAVSAGLATLRRLDDQAYSRLGVLTDRLVTGLKDLADEHGLTVA
                     SAPGLVTLFFSALPVKDFEDAKATDRERHAAFYRAMLERGIWLPPSQFEACFVSLAHD
                     EIMIDRTLMAAAEALAATDPRRAVV"
     gene            7189..7767
                     /locus_tag="PLJ64_07995"
     CDS             7189..7767
                     /locus_tag="PLJ64_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93461.1"
                     /translation="MSPSSALDRLADRVREEGSPLTVREPGQASAFGELAAAGPRTSE
                     RAGDYAFVVEAVREGYLCHYGESRILEMPDPDLALLAGDLFYAIGIRGLAELDDLEST
                     GILSDLIRVAAELEAAGRPDLAETLWVAQIVALACGKDDQLSSATAALEAGQTDAARA
                     VREWSIETAAANGLGREFDLAQTAIDSRPSNF"
     gene            7820..>8353
                     /locus_tag="PLJ64_08000"
     CDS             7820..>8353
                     /locus_tag="PLJ64_08000"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HNC93462.1"
                     /translation="MKLPPSEPRGYFEGESMTRRHVFTVASQAIGGVAAAAVVLPVVG
                     FAVAPLFERGEERWEAVGPLSDFNAETYKQVVITETPGIGEAGKTSAYVRTGAEKYNE
                     EPNDVIAVSTRCAHLGCPVRFVQAAQNFICPCHGGVYDFEGKRIGGPPVRPLDRFQTR
                     VRGGQVELGPRYSVTNQL"
BASE COUNT         1339 a         2841 c         2850 g         1323 t
ORIGIN      
        1 gccagcaggc cgaccgcaag cacgccggtc tccgcgtcct ccgctagccg cccggttccg
       61 ccatgggaaa aaagctggtc ctcgcctatg tcgattcgct tcgaaccgac atgctcaaac
      121 agaccatcga ggagagccgc gcgcccacct tcaggcgcct gctgaagcgc ggcaccctga
      181 tcgaggactg cgtttcatcc ttcccctcgg taacccccgt ggcttcggcc gagatggtca
      241 ccggggccca ggcggaccga cactggatca gcggcatgaa ctggtttcac cgggccgagc
      301 agcgatacgt ggagtacggg tcatcgctcg aggcgacccg ggccttcggc ctgttccgct
      361 cgctgcatga cctcgtctac aacatgaacc tgagccatct cagcccctcg gtcgaaacgg
      421 ttttcgaaac cctcgatgac gccgacatcc ggaccgcgtc cacgccgttt ctgatctacc
      481 gcggccgcca tcgccacgag gtgaacatcg ccgggatcac cggcaaggcg atcgaggccg
      541 ggctgctcaa gttcgattac ggcacctggg ggccgcggga actcttctac ggcgacctct
      601 acacctcgat ggaaacgaag tgcaagccga gctcgatccc cggcaagcgt gacatgtact
      661 cggcctgctg cggggccgag ctgatctccc gggacgcctt cgacttcatg ctgctggcct
      721 tccccgacaa cgacaactac tcccaccggc acggcccgct ggcctcggtc cgctcgatcg
      781 aaagagccga ctactgcatg gccgaggtga tccgcgcggc cggcggggtc gagcctttcc
      841 tccaggacca cgccctgatc ctgctggccg accatgccca caccgcggtc gagcaccgtt
      901 acgacatcat cgagtggatg agccgcaagt ggaaggttct gccgccttcc tccgaggagc
      961 cggccgaggc cgagctggcg gtcagcccga cttcccgcgc cgcccacata tatctgctgg
     1021 ccgaacagga caacccgatc cggcaccggc gggttcgcga gcatctggtc ggcctcgaag
     1081 ggaccgaact ggtcagctgg accgaaaggg acggccagcc ggtcgttcgc tcgacccgcc
     1141 ccggccacct cgaaccgggc aacgtcgcgg tcgtggaaag ggacggtcgc cggctcgact
     1201 tcttccccct gagttcgggg gcaaatggcg acgggcaggg agaggtgccg caaaaacagc
     1261 cccgtgacgg ggcaaaacgt gcggcacctt ccccgggtga gggccgtgaa acggtgaccg
     1321 accggcgcgg gcgacagtgg tcgatcagcg gcaacatcga ggctctcggg gcctcggtcg
     1381 aggagggccg gatcgagacg cccgaatacc cggacgggct cggccggctc tggtcagccc
     1441 tcgtcgcccc ccatggcgga gacatcatcg tctccgccgc gcccggctac gaatgcgtcg
     1501 actggggcgg agccgcccac gtgcccggcg gcagccacgg ttcgctccgc aaggaggatt
     1561 ccgaaggccc gctgctcttc gtcggctgcg gccccgactc accggacccg gaaaccgccg
     1621 cccgccaccg gtcccgaccc caatggggcc tccaggacgt tgccccgata atcagggaat
     1681 tcttcggcac ctgagaggag tcgggcaggg tccgacccgc cctccgctag gacgagggtt
     1741 cgaggatttg cttgaacccg gttgcgaggc cgttttcgtc gatctcgacc gcggcgccca
     1801 ttacccagac atcatcggtg gcggattcga agcggatcgg catgtcggtt cgcatcttct
     1861 cgatcgcctg ttccttcttc actccgagga ccgaatcgcg ggcgccggtc atgccgacgt
     1921 ccgagatgaa ggccgtgcct ccgggaagca cccgcccgtc ggcggtcggc acgtgggtgt
     1981 gggtgccgaa cacggcggcg acccggccgt ccagatacca gcccatcgcc accttttcgc
     2041 tggtcacctc ggcgtggaag tcaacgacca ccgtgtcggc tccgccgctc ttctcgaggt
     2101 cgttcaggat caggtcggcc tcgtcgaaag gtgaccgggc ggcgtcgagg ccgatcttgc
     2161 cgatcaggtt gatcaccgcg acccgtcggg ccggcgagcc gtcggccggg ttcacctgga
     2221 ccacgcagtg gcccttgccg ggattggccc gccggtagtt ggcggggcgg atcacccgct
     2281 cggcccggtc caggtaatcc cagctttcgc gctggcggta ggtgtgattg ccaagcgtga
     2341 tcacgtcggc gccgcaggcg aagatctcct ttgcggtctt ctcggtgatt ccgaggccgc
     2401 cggccgagtt ctcgccgttg accacgatca ggtccggcga atgttcttcg cgcagtcgcg
     2461 gcatcagcga agccagcccc ttgcggccgg gactcccgac gacatcaccg atgaagagca
     2521 gcttcacgct tgcgagccta aaccttgggt aggttcaacc tcgtgcagac accgctcaca
     2581 ggcacaggcc ccgccctcgc ggcgcggggg gtcagcaagt ccttcgatgg acggaaggcg
     2641 ctcaggggag tcgacttcga ggtcgggacc ggcgaggtcg tggcgctgat cgggccgaac
     2701 ggcgccggca agacgacgct gctttcgatc ctggcaggca tcgccgaacc cgactcgggc
     2761 gaggtgaccg gaaacggtgt cgcctgggtc ccgcagcagg cggcgctata ccggcggctc
     2821 ggcgtgcgcg agaacctgct gctgttcgcc cgcctccagc acgatgcggg gcagaccggc
     2881 gaggccccgg agcagatggt cacccggatg ctcgagctaa ccgggctcgg tgaccgcgcc
     2941 ggcgaccagg tcgggactct ctccggcggc aaccagcagc gggtcaacat cgcgatcggc
     3001 ctgatggccg gcccctcggt gctgctgctc gacgagccca gcgtcgggtt ggacccggcc
     3061 cagcgggagc ggctctgggg gttcgttctc ggcctggtcg gcgagggcac cgccgtcgcc
     3121 ttctcgaccc atgacatcca ggaagccgag cgctacgcgg agcggatcgt ggtggtggcc
     3181 gaaggcgagg gcatcttcag cggcaccgcc gacgcgctcc gccaggcggt ggaggtcgag
     3241 ggcggaccgg ccggcgacct cgaaacgtcc ttcctcgcct acctccgggg gcgcggtcac
     3301 tgatgcgctg gctgctcctc aaggacctgc agatcctcaa gcgttcgccg cttcagctca
     3361 ccctgctgat cctctatccg atagtgatcg ccctgctggt cggcttcgcg atgacccgcg
     3421 accaggacca gaccaaggtc gccctctaca acgcgatgcc ggccggcgag ccgctcggct
     3481 tcggcgggga ggaggtcgac ggcaacgaga tccgcgacca gctctgcggg agggtcgatt
     3541 gcgttgaagc cgacagcacc gcggacgcga agaagatggt cgagagcggc gaggccaccg
     3601 ctgcggtgat cgtgccggaa gacctcggcg acaagatccg ctccctcgcc tcgctcaacc
     3661 ccgagtcacc ggagatccag atcatcgtca acggctccga cgcggtcgaa agccgggtca
     3721 ccgacgaccg gatcgaagcg atgctcggcg aggcgaacct gctgctggcc aagcgcctct
     3781 ccgaaggcgc gaaggagtat ctcgacctga tcctccaggg cggcgagttc gacctgctcg
     3841 gccagcagat cgaggttctc gggctgaaga ggagcgagca gatcctcaag cagatcgagc
     3901 cgaagctgcc gccgggaacc gacagggagc agctcggccg ggcggtccgc ttcgcccacc
     3961 tggcgaccga caacctggac ctggccggtc cgcttttgac cgcgatcacc gaaccgatca
     4021 aagccgagaa ggtcgaggtc ggtgatgacg cgccctcgct ggacaccttc gcaatcgggg
     4081 tgaccgtcgc cttcgcgctg atgttcgtga ccgtcctgct ggtcgccgga tcgctggcgc
     4141 tcgaaaagga ggagaacgcc ttcacccggc tcaccgccgg tctggtcagc cgcggccaga
     4201 tcctcgtgag caaggtcggt ctgggaaccg tggtcggctt cgtggtggcc ctgattctgc
     4261 tgaccgggct ggggctgttc gtcgggatcg agtgggggag agcgccctac tggctgctcg
     4321 ccctgatctt cggcgcggcg gctttctcgg ctggcggcgc ggcgctgggt gccgcggccc
     4381 gggaggtgcg ggcggcgacc ctggctgcgg tgatgatctg cctgccggtt gctctgctct
     4441 ccctggttcc cgacgacgcg gtcggcccgg tgatcgaaac cgcgatcaac ctggtcagcg
     4501 cgatcttccc cttccgccca accctcgacg cgatcaccgg cggcctcgag atctccggtc
     4561 caaccctctg gctccccctc ctccacctgg tcatcctcac cgccgcctac ggagcgctgg
     4621 cccgcctcgc cctacgccgt ttttgactta actgccagtc gggcggggtc cgacccgccc
     4681 tccacgagga acccgcccct cccattcgag acaactgatg gttgggaggg gctcagcctg
     4741 aagtacttct agactggact tatgagcttt ccgagtaccc gcctgcgccg tctgcgccgc
     4801 accccggccc tccgcgggct ggttcgtgaa accggcctgt cggcggccga cctgatccag
     4861 ccgctcttcg tcacggccgg caccgacctg cgcgaaccgg tcgagtcgat gccgggggtc
     4921 aaccggttct cgatcgccgc cctggttgag gaggcggggg agattcaggc ggcggggatt
     4981 ccggcggtgc tggtcttcgg ggttccctcg gagaaggacg aggcgggcag cggggcctac
     5041 gacgacgagg gcgtggtcca gatggcggtg cgggccctga aggagagcca cccggacctg
     5101 atcgtgatca ccgacgtctg cctttgcgaa tacacggatc acggccagtg cggatttgtc
     5161 cgcgacgggc aggtcgacaa cgacgtgacg gtcgagttgc tggcgcggac cgcaatttcc
     5221 catgcggagg ccggggccga catcgtcgcg ccgagcgaca tgatggacgg ccggatcggg
     5281 tcgatccgct accagctcga cgaggaaggc caccccgaca cggcgatcct ctcctacgcc
     5341 gccaagtacg cgtcggcgtt ctacggaccg ttccgcgagg cggccgaatc aacccccgct
     5401 ttcggtgatc gtcgcggcta ccagatggac ccggccaact cggacgaagc ggtccgcgag
     5461 gcgaagctcg acctcgagga gggcgccgac gcgctgatgg tcaaacccgc cggccactac
     5521 ctcgacatcg tccgccgggt caaggacgag accggcgccc cggtcgcggc ctatcaggtg
     5581 tccggcgagt actcggcgat cagggcggcc ggggccaacg gctggatcga cgagcgggcc
     5641 gcggcccttg aatcactggt ctcgatccgg cgggccggag cagatttcat cgtcacctac
     5701 ttcgccaggg aagcagccgc gtggctggct gaatcgtgag gagtttcatt gtgaggagaa
     5761 gcggatgtca ggtctgagcc ggggtgcccc cgacaaggtc acgatcaagg tgcgcggcga
     5821 cggcagcccg ctggccgaga ccggttccga ggtcgaatac cgggaagagg agaaggcgaa
     5881 agccgcggcg gcacaggcgg ctgccggacg ggccggcaac gaggagctat tcgagcgggc
     5941 ccgccgggtc ctgccgggag gggtgaattc accggtccgg gcgatgggcc aggtcgggat
     6001 cgaccccgtc ttcatcgaat ccgccagagg cgcggaagtg ctcgaccggc aaggccgccg
     6061 ctatctcgac tgggtgagtt cctgggggcc gatgatcctc ggccacgccg accccgaggt
     6121 cctggcggcg atcgaggagg cggccggacg gggaacgagc tttggcgcgg caaccgagct
     6181 cgaggtggaa ctggccgaag aggtggcaga gcggatgcct tcggtcgaga tgatccgcat
     6241 gaccagttcg ggaaccgagg ccgcgatgac ggcggcccgg ctggcccggg cggccacggg
     6301 tcgcgaggtg ctggtcaagt tcgccggcgc ctaccacggc cactccgacg ggttgctggc
     6361 ggaagctggc tcgggggtcg cgacccacgg cattccttcc ggccccggaa ttcccgaagc
     6421 ccaggcagcc ggcaccgtgg tcatcccctg gaatgaccgc gaggcggtgg ccgaggccct
     6481 cgagaagcac aaggtggcgg cgcttttcgc cgaacccgtg gcggccaaca tgggagtcgt
     6541 gcctccggag gagggctacc tcgaattcct gcggcaggcg accgcccggg ccggggcgat
     6601 gctggtcttc gatgaggtga tcaccgggtt ccgggtggcc cgcggcggcg cccaggagct
     6661 gttcgggatc gttcccgacc tgaccgtgat gggcaagatc atcggcggtg gcctgcctgc
     6721 cgccgcggtc ggcggctcgc gcgagctgat ggagaggctc gccccgaccg gtgacgtcta
     6781 ccaggcgggc accctttccg gaaacccgtt ggcggtgtcc gcgggactgg ccaccctgcg
     6841 acgccttgac gaccaggctt acagccggct cggcgtcctg accgaccggc tggtcacggg
     6901 cctgaaagac ctcgccgacg agcatggcct caccgtggcc tccgcgccgg gactggtcac
     6961 gctcttcttc tcggccctgc cggtcaagga cttcgaggat gcgaaggcga ccgaccgtga
     7021 acggcacgcc gccttctacc gggcgatgct ggaacgcggg atctggctgc cgccgtcaca
     7081 gttcgaggcc tgtttcgtct cgctcgccca cgacgagatc atgatcgacc gcacgctgat
     7141 ggcagccgcg gaagccctgg ctgccaccga tcctcgcagg gccgtcgttt gagtccgtcg
     7201 agtgccctcg accggctcgc cgaccgggta cgcgaggaag gatcacccct gacggtcagg
     7261 gagcccgggc aggcttcggc cttcggtgag ctcgcggccg cgggcccccg aacctcggaa
     7321 cgggccggcg attacgcctt cgtggtcgag gcggttcgcg agggttacct ctgccactac
     7381 ggcgagtcgc gcatcctcga gatgcccgac ccggatctcg cgctcctggc cggagatctc
     7441 ttctatgcga tcggcattcg cgggctcgcc gaactcgacg acctcgaatc cacaggaatc
     7501 ctgtcggatt tgatccgggt cgcggccgag cttgaggcgg ccggccggcc ggacctggcg
     7561 gagaccttgt gggtagccca gattgtcgca ttggcatgcg gaaaagacga ccagctgagt
     7621 tccgcgacgg cggccctgga ggcgggtcag acggacgccg ccagggcggt cagggagtgg
     7681 tcaattgaaa cagcggctgc caacgggctg gggcgggaat tcgacttggc gcagactgca
     7741 atagactccc gcccgtcgaa tttctagaag cgtgtccgaa ccgttgaaca acaattccga
     7801 gtcgggggaa gagccccgcg tgaaactgcc cccgagtgag ccccgtggtt acttcgaggg
     7861 cgagtcgatg acccgtcggc atgtcttcac cgtcgccagc caggcgatcg gtggggtggc
     7921 cgcggcggcc gtcgtgctgc ccgtggtcgg gttcgcggtc gcgccgctct tcgagcgcgg
     7981 cgaggaacgc tgggaagcgg ttggcccgct cagtgacttc aacgccgaga cctacaagca
     8041 ggtcgtgatc accgagaccc cgggcatcgg cgaggccggc aagacctccg cctacgtccg
     8101 caccggcgcc gagaagtaca acgaggagcc gaacgacgtg atcgccgtct cgacccgttg
     8161 cgcccacctc ggctgcccgg tccgtttcgt ccaggccgcc cagaacttca tctgcccctg
     8221 tcacggtggc gtctacgact tcgagggcaa gcgcatcggc ggcccgccgg tccgtccgct
     8281 cgatcgtttc cagacccggg tccgtggcgg ccaggtcgag ctcggtcccc gctactcggt
     8341 gaccaaccag ctc
//
LOCUS       DAOQKN010000107         8306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_4157_length_8306_cov_5.670828, whole genome shotgun sequence.
ACCESSION   DAOQKN010000107 DAOQKN010000000
VERSION     DAOQKN010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 8306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8306
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4157_length_8306_cov_5.670828"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..1761)
                     /locus_tag="PLJ64_08005"
     CDS             complement(121..1761)
                     /locus_tag="PLJ64_08005"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNC93463.1"
                     /translation="MPALTRFRGRRYGFALAALLGAALVLLLVAPLDRSLAARPNFVV
                     IQTDDQNAATVRARLRGKVGGFKPVMPHAVRQILNRGTEFRNYYASSPVCSPSRASLL
                     SGQYPWNNGVESNSGPNGGWNGWVNNPVYRQNLVTTLHRSGYRTAHFGKFTNGYFDTT
                     HGSVERKVPPGWTDWFTTSYEVGTHFYGYRLNVNGRATRPIGSLHYRQYGPGIDPRKC
                     SLRKLEDFWPLGDCNYSGDVFTAAAVREIRRENRRPFYIQIDYQAPHGDVAAPRGPQP
                     ASREIGTARRTPLPRPPSFNEADFSDKPALIRDLAPDPMGPGAIRRLTFSYRRYLESL
                     RSVDDGVGAIMRALRQSGKLGRTYVFLLSDHGLFLGEHRYDWGKFMPYEDSASPLMAV
                     RGPGVRRGRVSREVVGNIDVPVTIMRLARARPGYAVDGRSLRPYWRHPGRQTNRAMAI
                     AIDSRQDDGSASASAHAPALRYRGFRAGRYKYIRYDQGGEELYDLARDPNEMNNRAGA
                     PAYSGVLAWMRARLNRVTECQAGVCRAPLPEPPRPSRS"
     gene            complement(1767..2453)
                     /locus_tag="PLJ64_08010"
     CDS             complement(1767..2453)
                     /locus_tag="PLJ64_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNC93464.1"
                     /translation="MPLPSLKNIEWVTTDCYGDLIDWEKGISEACLKEAEKDGFSFDP
                     GPFMERFFEVQAEIMAGSYELYAEVLRRTIIKVAGEIGWEVEPSRAQFLPDSVAYWMP
                     FREANAAMDRLAKKYNIGIISNIDDKMLGISRRHLRTDLDLVVTAQQVRSYKPDDTHF
                     KECARRIGGKKGWLHIGSNYDTDVAPLLKMKVPVIWVNRHGEKLAGRKKPSLEVKTFR
                     DAVKKLGAGG"
     gene            2562..3722
                     /gene="sucC"
                     /locus_tag="PLJ64_08015"
     CDS             2562..3722
                     /gene="sucC"
                     /locus_tag="PLJ64_08015"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HNC93465.1"
                     /translation="MDLLEYQGKELFARHGLPVPKGVHAKTADAAVDASEALGYPVAV
                     KAQVLIGGRGKAGGIKIAKDGTEAHEYAGEIIGMDIVGPHGEGPFKVHELWVEAGSEI
                     ESEFYASVILDRSAKKLMVMLSRMGGMDVEAIAASDPEALVKHHVDPLGGLGEDEAKQ
                     IAAQAKIPAEVADQVAAVLVQLVEVARAEDATLIEVNPLIVNADGKVVLLDAKVTIDG
                     NAMFRHPELEELPESADQDPQEKMAKERGLTYVSLDGNIGILGNGAGLCMSTLDVVAQ
                     AGGRPANFLDAGGGSKAEAIIDALEVITSNPEVTAILFNIFGGITRCDEVARGIIEAT
                     NKIGLTLPLVVRLDGTNSEEGLALLAEAGLPNLHHEATMLGAAKKVVELAGN"
     gene            3732..4607
                     /gene="sucD"
                     /locus_tag="PLJ64_08020"
     CDS             3732..4607
                     /gene="sucD"
                     /locus_tag="PLJ64_08020"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNC93466.1"
                     /translation="MSIIIDNDTKLVVSGLTGREGSFHGLRNKAYGTRLVAGVTPGKG
                     GQDVEGTPVFNTIEESVSEAGANTAMIFVPAKFAAGSINEAIDSGVDTVIAITEGIPV
                     MDMLPTYWKAREKGVRLIGPNCPGVLSPGKANVGIIPAQFFDQGRIGVVSKSGTLTYQ
                     IGNELKQAGEGNSTIVGIGGDPIIGSDFIDILTLFDADPETDLIVMVGEIGGDAEERA
                     AEFIAAEISKPVVAYIAGFTAPPGKQMGHAGAIISGSSGTAKAKQEALEAKGVRVGES
                     PTEAARIAVETLRSM"
     gene            4674..6335
                     /locus_tag="PLJ64_08025"
     CDS             4674..6335
                     /locus_tag="PLJ64_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93467.1"
                     /translation="MSPFRQRIDRLRQGENSPRAPWLVLAGAMALASGFILYYGRDGW
                     FSIDEAQWISDSPQLDLGGALSPHVGHLVFIPRLVYKLMLETVGIEYIAYRILTVLAI
                     LLCTALFFTWARRRLPDFVALAFSLPILLFAYDPLHLIAGNGFTVMLALSFGIAALLA
                     WDREDRTGDIWTFAFLVLGGLTYTVALPFSVAIAFAAVLDRRRDRLWVGAVPIVLYLA
                     WRTLGDVGATDTAAGGGDLLNILLLPAWSFGGISGVLASWSGLSYDFIRSGTMQPGTG
                     IGPALAVVAIAALGWRIATRGAGTKLWTVIAVAIALFAAQTIAVGGIERYPEMPRYMY
                     PGLIVVFLVVVEAASGFKWSRAAFIGLWLITVISLMTSFSLLRDQSEVREWKATQTRV
                     EVTAVGLLDQTPNPPPVIRQRRRMLTDDFNPVTGSAYGALGYDPATLKDRDPDIGNAV
                     DRFLFNALKIGFQPVTGSFSRADCRPLEAIGGRYRMWVPRGGAVVIGKQDAKVWIGRF
                     GTGRKLRAGRVGPNSPQLLKLRYDGEPTPWFLKSKAPGVSVCPID"
     gene            6383..7453
                     /locus_tag="PLJ64_08030"
     CDS             6383..7453
                     /locus_tag="PLJ64_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNC93468.1"
                     /translation="MPDTISFARGAPSPDLIPVEAVRVAAAKALEDDWQKALSYGVGI
                     GHPGLCAWIADRHGIDPSQVMTTNGSLEAGAMLFRHLLSPGDRVVVEQPSYDRTLLLL
                     EQIGVVFVPIALEEDGIDVDALEKALELGPIKLAHIIPNFHNPAGCTLSEEKRRRLVA
                     LAAEHDFVIFEDDPYRELPFGEDPPPTMLSMDTADKVIHASSFSKTVSPGVRVGYLAG
                     PEHEIARLAKIANENYISPNMLSGSLVLELCQSGVLDRNIAEVKVALQERRDALVEAL
                     GKHIPEATFVLPGGGYFLWADLAEDRDTVQIAAKAKEAGAPFIPGVDFMLDAGQRSLR
                     FSFASVPPEHMDEGVSRIASCL"
     gene            7581..7811
                     /locus_tag="PLJ64_08035"
     CDS             7581..7811
                     /locus_tag="PLJ64_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93469.1"
                     /translation="MSKMVQIRDVPDEIHALLVERAASEGRSLSEMLREEVATLARRP
                     SRAEVLKRIRARGRIETPETGAEAIRRLREDD"
     gene            7808..8194
                     /locus_tag="PLJ64_08040"
     CDS             7808..8194
                     /locus_tag="PLJ64_08040"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNC93470.1"
                     /translation="MIVVDASVILDLLRLAEGSTSTEIALVSGEPLAAPDHMLVETTR
                     VLRRWQLGGAFDAETASEMVIQALDLGIRSYSCGDLIPRAWELRNQFTLDDALYIALA
                     EALPARLLTSDQKLASQASRFVEIYE"
BASE COUNT         1399 a         2793 c         2708 g         1406 t
ORIGIN      
        1 cggattcgaa agcaccgcca cccttgatca ggccttcaag tactaccggg tggtcggcct
       61 ctcggccggt ggcaaggtgc tcggccgctc ggaactcaac cggctcggcc ggctgaccga
      121 ctagctccgc gacggccggg gcggctccgg caggggtgcc cggcagacac cggcctggca
      181 ttcggtgacc cggttcaggc gggcccgcat ccaggcgagc acgccggaat aggccggtgc
      241 cccggcccgg ttgttcatct cgttcgggtc ccgggcgagg tcatagagct cctcgccgcc
      301 ctggtcgtaa cggatgtact tgtaacggcc ggcccggaag ccgcggtagc ggagggcggg
      361 agcatgcgcg gaggcggagg cgctgccgtc atcctggcgg gagtcgatcg cgatcgccat
      421 ggcccggttg gtctggcggc ccggatgccg ccagtacggg cgaagcgagc ggccgtccac
      481 cgcatagccc ggccgggccc gggccaggcg catgatcgtc accgggacgt cgatgttgcc
      541 gaccacttcc cggctgaccc ggccgcgcct cactccgggt ccgcgcaccg ccatcagcgg
      601 cgaagcggaa tcctcatagg gcatgaactt gccccagtcg tagcggtgct cgccgaggaa
      661 gaggccgtgg tcggagagca ggaagacgta ggtacgcccc agctttccgc tctgccgcag
      721 cgcccgcatg atcgctccca ccccgtcatc gaccgagcgc aacgattcga ggtagcggcg
      781 gtaggaaaag gtcagtcgcc gaatcgcgcc ggggcccatc ggatcggggg caagatcccg
      841 gatcagcgcc ggcttgtcgc tgaaatcagc ctcgttgaat gagggcggac gcggcaacgg
      901 ggtccgccgg gcggtgccga tctcgcggct ggccggttgg ggtccacggg gcgcggcaac
      961 gtcaccgtgg ggtgcctggt aatcaatctg gatgtagaag ggccggcggt tctcacgcct
     1021 gatctcccgc accgcggcgg cggtgaagac gtccccgctg tagttgcagt cgccaagcgg
     1081 ccagaaatcc tcgagtttcc ggagcgagca tttccggggg tcgatccccg ggccgtattg
     1141 ccggtaatga aggcttccga tcggccgggt ggcgcgaccg ttgacgttca gccggtagcc
     1201 gtagaaatgc gtcccgactt catatgaggt cgtgaaccag tcggtccagc ccggcgggac
     1261 cttcctttcg acgctgccgt gggtcgtgtc gaagtagccg ttggtgaact tgccgaagtg
     1321 ggcggtgcgg taaccggagc gatgcagggt ggtgaccagg ttctggcggt agaccgggtt
     1381 gttcacccag ccgttccagc cgccgttcgg gcccgagttg gactcgaccc cgttgttcca
     1441 cgggtactgg ccgctcagca gcgaggcccg ggaagggctg cagaccggcg agctcgcgta
     1501 gtagttgcgg aactcggtgc cgcggttgag gatctgccgg accgcatggg gcatgaccgg
     1561 cttgaagccg ccgaccttgc cgcgcaacct ggcccggacc gtggccgcgt tctggtcatc
     1621 ggtctggatc accacgaagt tcggcctggc cgcaagcgag cggtcgagcg gggcgaccag
     1681 cagcagcacc agcgcagcac ccagcagggc cgccagagcg aacccgtacc gcctcccccg
     1741 gaatcgggtg agggcgggca agttggtcag cctccggcgc ccagcttctt gacggcgtcg
     1801 cggaaggtct tgacctccag cgagggcttc ttgcgtccgg ccagcttttc gccgtgccgg
     1861 ttgacccaga tcaccgggac cttcatcttc agcagggggg ccacgtcggt gtcgtagttc
     1921 gaaccgatgt ggagccagcc cttcttgccg ccgatccggc gggcacattc cttgaagtgg
     1981 gtgtcatcgg gcttgtagct gcgaacctgc tgcgcggtaa cgaccaggtc gaggtcggtc
     2041 ctcaggtgac gccgcgagat gccgagcatc ttgtcgtcga tgttgctgat gatgccgatg
     2101 ttgtacttct tggccagccg gtccatcgcc gcgttggcct cgcggaacgg catccagtag
     2161 gcgacactgt cgggcagaaa ctgcgcccgt gaaggctcga cttcccagcc gatctccccg
     2221 gcaaccttga tgatcgtgcg cctcagcacc tcggcgtaga gctcgtagga gccggccatg
     2281 atctcagcct gcacctcgaa gaaccgctcc atgaacgggc cgggatcgaa gctgaatccg
     2341 tccttttcgg cttccttcag acaggcctcg gagatgccct tctcccagtc gatcaggtct
     2401 ccgtagcagt cagtggttac ccactcgatg ttcttcagtg atggcagcgg catgcgggaa
     2461 aggcattatg tcgaatagag tccatcgtcg gcttcgacgt ggcccaaagt accttcggcc
     2521 atgccttccg gagccctcca actcccaact cctcaaaggt catggatctc ctcgaatatc
     2581 agggaaaaga actgttcgcc cgccacgggc ttcccgtccc caagggcgtg cacgcgaaaa
     2641 ccgccgacgc tgccgttgac gcttccgaag ccctcggcta cccggtcgcc gtcaaggccc
     2701 aggtactgat cggcggccgc ggcaaggccg gcggcatcaa gatcgccaag gacggaaccg
     2761 aagcccacga gtacgccggt gagatcatcg gcatggacat cgtcggcccc cacggcgaag
     2821 gcccgttcaa ggtccatgaa ctctgggtcg aggccggttc agagatcgaa tccgagttct
     2881 acgcgtcggt catcctcgac cgctcggcca agaagctgat ggtcatgctt tcccgcatgg
     2941 gcggcatgga cgtcgaggcg atcgccgcct ccgatccgga ggctctggtc aaacaccatg
     3001 tcgacccgct tggcggcctg ggtgaagacg aggcaaagca gatcgccgcc caggcgaaga
     3061 tcccggccga ggtcgcggat caggtcgctg cggtgctggt ccagctggtc gaggtcgccc
     3121 gggccgagga cgcgaccctg atcgaggtca acccgctgat cgtcaacgcc gacggcaagg
     3181 tcgtgctgct cgacgccaag gtgacgatcg acggcaacgc gatgttccgc cacccggaac
     3241 tcgaggagct tcccgagtcc gccgaccagg atccgcagga gaagatggcc aaggaacgcg
     3301 gcctcaccta tgtgtcgctg gacgggaaca tcggcatcct cggaaacggc gccgggctct
     3361 gcatgagcac gctggacgtg gtcgcccagg ccggcggccg ccccgccaac ttccttgatg
     3421 ccggcggcgg atcgaaggcc gaggcgatca tcgacgcact cgaggtgatc acctcgaacc
     3481 ccgaggtcac ggcgatcctc ttcaacatct tcggcgggat cacccgctgc gacgaggtcg
     3541 cccgcggcat catcgaggcc accaacaaga tcgggctcac cctgcccctg gtggtccgcc
     3601 tcgacggcac caactccgag gaaggcctgg ccctgctggc cgaagccggc ctccccaacc
     3661 tgcaccacga agccaccatg ctcggcgcgg ccaaaaaggt cgtcgagctg gccgggaact
     3721 gaggatcagc aatgagcatc atcattgaca acgacacgaa actggtcgtc tccggcctga
     3781 ccggccgcga gggcagcttc cacggcctcc gcaacaaggc ctacggcacc cggctggtcg
     3841 ccggtgtcac ccccggcaag ggcggccagg acgtcgaagg cacccccgtt ttcaacacga
     3901 tcgaggaatc ggtcagcgag gccggcgcca acaccgcgat gatcttcgtc ccggcgaagt
     3961 tcgccgccgg ttcgatcaac gaggcgatcg actccggggt tgacacggtg atcgcgatca
     4021 ccgagggcat cccggtgatg gacatgctgc cgacctactg gaaggcccgc gagaagggcg
     4081 tccgcctgat cgggccgaac tgccccggcg tgctttcgcc aggcaaggcc aacgtcggca
     4141 tcatcccggc ccagttcttc gaccagggca ggatcggcgt ggtctccaag tcgggcaccc
     4201 tgacctacca gatcggcaac gagctgaagc aggcgggcga gggcaactcg accatcgtcg
     4261 gcatcggcgg tgacccgatc atcggctccg acttcatcga catcctcacc ctcttcgacg
     4321 ccgaccccga gaccgacctg atcgtgatgg tcggcgagat cggcggcgac gccgaggagc
     4381 gggcagcgga gttcatcgcg gccgagatca gcaagcccgt cgtggcttac atcgccggct
     4441 tcaccgcccc tcccggcaag cagatgggtc acgcgggcgc gatcatctcc ggctcctccg
     4501 gcacggccaa ggcaaagcag gaggcgctcg aagcgaaggg cgtgcgggtc ggcgaaagcc
     4561 cgaccgaggc cgcccggatc gcggtcgaaa ccctgcgatc gatgtagttt tccgcggcgg
     4621 ggccttacac acggcgttta catccggaag ccgaaacccg cgaaaatacc ccggtgtctc
     4681 cgtttcgcca gagaatcgat cgcctgagac agggcgagaa cagcccccgc gcgccctggt
     4741 tggtgctcgc gggtgcgatg gccctggcga gcgggttcat cctctactac gggcgtgacg
     4801 gctggttctc gatcgacgag gcccagtgga tctccgacag cccgcaactc gacctcggcg
     4861 gggcgctttc cccccacgtc gggcatctgg tcttcattcc gcggctggtc tacaagctga
     4921 tgctggagac ggtgggcatc gagtacatcg cctaccgcat cctcaccgtg ctggcgatcc
     4981 tgctctgcac ggccctcttc ttcacctggg cccggcgccg gctccccgac ttcgtcgccc
     5041 tggccttcag cctgccgatc ctgctcttcg cctatgaccc gctccacctg atcgccggca
     5101 acggtttcac ggtgatgctc gccctctcct tcgggatcgc cgcgctcctg gcatgggacc
     5161 gcgaagaccg aaccggggac atctggacct tcgccttcct ggttctggga ggtttgacct
     5221 acacggtcgc cctgcccttc tcggtggcga tcgcgttcgc cgcggttctc gaccgccgcc
     5281 gggaccggct ctgggtggga gcggtgccga tcgtcctcta tctcgcctgg cggaccctgg
     5341 gtgatgtcgg tgccaccgac accgccgcgg gcgggggcga cctgctcaac atcctgctgc
     5401 tgccggcctg gagcttcggc ggcatctccg gtgtgctcgc ttcctggtcc ggcctgagtt
     5461 atgacttcat tcgctccggg accatgcagc cgggaaccgg gatcggcccc gccctcgcgg
     5521 tggtggcgat cgcggcgctc ggctggcgga tcgccacccg cggagcgggc acgaagctct
     5581 ggacggtgat cgccgtcgca atcgcgctct tcgccgccca gacgatcgcg gtcggcggga
     5641 tcgagcgcta cccggagatg ccgcgctaca tgtatcccgg cctgatcgtg gtgttcctgg
     5701 tcgtggtcga ggcggccagc ggcttcaagt ggtcacgggc cgcgttcatc gggctctggc
     5761 tgatcacggt catcagcctg atgacctcgt tctcgctgct tcgcgaccag tccgaggtca
     5821 gggagtggaa ggcgacgcag accagggtcg aggtgacggc ggtcggcctg ctggaccaga
     5881 ccccgaaccc gcccccggtc atccggcagc ggcggcgcat gctgaccgat gacttcaacc
     5941 cggtgaccgg ttccgcctac ggcgccctcg gctacgaccc ggcgaccctg aaggaccggg
     6001 atccggacat cggcaacgcc gtcgaccgct tcctcttcaa cgcgctgaag atcggtttcc
     6061 agccggtgac cgggtccttc tcgcgggcgg attgcaggcc gctggaggcg attggcggcc
     6121 ggtaccggat gtgggtgccg cggggcggcg ccgtggtgat cggcaagcag gacgcgaaag
     6181 tctggatcgg ccggttcggc accgggcgga agctcagggc cggcagggtc gggccgaatt
     6241 cgccgcagct cctcaaactt cgctacgacg gggaaccgac tccctggttc ctgaagagca
     6301 aggcccccgg cgtctcggtc tgtccgattg actaacatcg cctctcccgc ccagcgaccg
     6361 ctccgagagc gagagtgacg aaatgcctga cacgatcagt tttgcccgag gtgcccccag
     6421 tcccgacctg attccggtcg aggcagtccg cgttgccgcg gcgaaggcgc tcgaggacga
     6481 ctggcagaag gcgctctcct acggggtcgg gatcggccat cccgggcttt gcgcctggat
     6541 cgccgaccgt cacggcatcg acccctccca ggtgatgacc accaacggtt cgctggaggc
     6601 cggtgcgatg ctcttccggc acctgctttc acccggtgac cgggtcgtgg tcgagcaacc
     6661 gagttacgac cgcaccctgc tcctgctcga gcagatcggg gtcgtcttcg tgccgatcgc
     6721 gctcgaggaa gacggaatcg acgtcgatgc gctcgagaag gccctcgaac tgggcccgat
     6781 caagctcgcc cacatcatcc ccaacttcca caacccggcc ggctgcaccc tctccgaaga
     6841 gaagcgccgc cggctggtcg cccttgccgc cgagcatgac ttcgtgatct tcgaggacga
     6901 cccctaccgc gagctgccct tcggcgagga ccccccgccg accatgctct cgatggacac
     6961 ggccgacaag gtcattcacg cctcatcctt ctcgaagacg gtctccccgg gcgtccgggt
     7021 cggctacctg gccggtcccg aacacgagat cgcccgtttg gcgaagatcg ccaacgagaa
     7081 ctacatctcg cccaacatgc tttccggttc gctggttctc gagctctgcc agtcgggtgt
     7141 tctggaccgg aacatcgcgg aagtgaaggt cgctctccag gagcgacgcg acgccctggt
     7201 cgaggccctc ggaaagcaca tccccgaagc caccttcgtc ctgcctggcg gcggctactt
     7261 cctctgggcc gacctggccg aggaccgtga cacggtccag atcgccgcca aggccaaaga
     7321 ggccggagcc ccgttcatcc ccggggtcga cttcatgctc gacgccggcc agcgcagcct
     7381 ccgcttctcc ttcgcctcgg tccccccgga gcacatggac gaaggcgtct cccgcatcgc
     7441 ctcctgcctg taggcacatt tcacgcacca acaggcatcc ggcccgagag gtgccgcaca
     7501 tccaggaccg taatgggcca aaacctgcgg cacctccgat tcattcccgg cgcatgcgaa
     7561 tcgcatgcta gccttggctc atgagcaaga tggttcagat tcgcgatgtg ccggacgaga
     7621 tccacgcgct cctggttgaa agggccgcca gtgagggtcg atccctttcc gagatgctgc
     7681 gggaggaagt tgccaccctg gcaaggcgac ccagtcgcgc agaggtgttg aaacggatca
     7741 gggcaagagg aaggatcgaa accccggaaa ccggggcgga agccatccga aggctgcggg
     7801 aagacgattg atagtcgtcg atgcgagtgt gatcctcgac ctgcttcgtc tggcggaggg
     7861 ctccacttcg accgaaattg cgctcgtttc cggagagccc ctcgccgcgc ccgatcacat
     7921 gctggtcgaa acaacgcggg tgcttcggcg ctggcagctg ggcggcgcat tcgacgcgga
     7981 gaccgcgagc gagatggtga tccaggcgct cgatcttggc atcaggagtt attcatgtgg
     8041 cgacctgatc cccagggcct gggagttgcg aaaccagttc accctggatg acgccctgta
     8101 tatcgccctt gcggaggcat tgccggcgag gctcctcacc tccgatcaaa agctggccag
     8161 ccaggcgtcc cggttcgttg agatctacga gtaggtctat gccctagcgg ccgtagcggt
     8221 cgtctgagag gacgagctgg tcggagacct atcggggaag aatccgcaca accgcaagtg
     8281 agctctcggc gggttctgat gctgcc
//
LOCUS       DAOQKN010000108         8283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_4177_length_8283_cov_5.468887, whole genome shotgun sequence.
ACCESSION   DAOQKN010000108 DAOQKN010000000
VERSION     DAOQKN010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 8283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8283
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4177_length_8283_cov_5.468887"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="PLJ64_08045"
     CDS             <1..765
                     /locus_tag="PLJ64_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNC93471.1"
                     /translation="LIEGGHEVEASTVGSVTNAIQSSRRPAEVLDDVVWHNRNTRVVP
                     KTVNQKVYVDSIRENTVTFGIGPAGTGKTFLAVAMAAAALIEGEVQRIILTRPAVEAG
                     ESLGFLPGDLQAKVDPYLRPLFDAIYDMLDPDRVNGYFDRGVIEVAPLAFMRGRTLND
                     AFVILDEAQNTTPEQMKMFLTRLGFNSKMVVTGDVTQIDLPRDSKSGLVVVREILEGI
                     EDVRFVRFGGKDVVRHRLVQRIVAAYGEHAERENGK"
     gene            762..1160
                     /gene="ybeY"
                     /locus_tag="PLJ64_08050"
     CDS             762..1160
                     /gene="ybeY"
                     /locus_tag="PLJ64_08050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNC93472.1"
                     /translation="MSLDLDDVPARFRPAVTATLEAAGVGNGHLAVEVVDEARIRKLN
                     RDHRGKNKPTDVLSFPVDGEEGPGPVELGDVVICPEFCVDEVEAVVHGVLHLCGYDHE
                     TDQGEMLDLQDRVVSGLGIEPKMTVEADDE"
     gene            1153..2061
                     /gene="era"
                     /locus_tag="PLJ64_08055"
     CDS             1153..2061
                     /gene="era"
                     /locus_tag="PLJ64_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNC93473.1"
                     /translation="MSEGSRSGFVGLAGRPNVGKSTLVNAIVGAKVAISSVRPQTTRR
                     AIRGVATDLEAGTQLVLVDLPGVQRPRDELTSRMQKTVEGELAGADVALLVINGEEGV
                     GPGDRFIAETLLGARAKAGGLPIICAVNKADRLGRNIVPVLVAAAELAGVDEVFPVSA
                     LTGQGVPELASYLASIVPEGPYLYPPEDHTDQPRELLLAELIRGQALIRTRDEIPHSV
                     EVRVTGIEQREDGLWLVDAEIWVESDSQKRILIGKKGFKVGEIGTGARKDIEGELGGK
                     VHLDLQVKVKRKWRRDEDMLDRLGID"
     gene            complement(2316..3266)
                     /locus_tag="PLJ64_08060"
     CDS             complement(2316..3266)
                     /locus_tag="PLJ64_08060"
                     /inference="COORDINATES: protein motif:HMM:NF016143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HNC93474.1"
                     /translation="MKWKRGRSGNRNVIDIRGAKRGGGGGGGGFGGIGGMGGGGMQLP
                     GGKGKIGGLVGVIIVIIVIGTQVLGGGGSGGGTGFDMGSMFPGMGAVPGAENPSPIPA
                     GEDPDADLKDFSAYVFTDAQQVWRKAFTRDGRRWQDAQLVLYSGAVSTGGCGNATSAV
                     GPFYCPADNRVYLDLSFYKDMKNQLGADGDFAWAYVIAHEMGHHVQNMGGISDEVSRE
                     KREQSESRNEISVRNELQADCLAGVWASTVFDQLEAGDLDEAIGASEAVGDDRLQSRG
                     GGSVNPDSFTHGTSAQRHRWFKTGFESGDPASCDTFSGDI"
     gene            3337..3768
                     /locus_tag="PLJ64_08065"
     CDS             3337..3768
                     /locus_tag="PLJ64_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93475.1"
                     /translation="MLRKVAICALIGSATLVSACGGSDDSTSSEDSQAITELVAKLNQ
                     ATQEKDASAFCLIMQPSAVEQTFHDIDRCVSETTPILKAAGDQGVLKIDTIEVNGDIA
                     AVTFQGGTSREAKFVKEDGQWYVPLSQSEITTNQGSNGSEG"
     gene            3768..4385
                     /gene="hisIE"
                     /locus_tag="PLJ64_08070"
     CDS             3768..4385
                     /gene="hisIE"
                     /locus_tag="PLJ64_08070"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HNC93476.1"
                     /translation="MPEVEVKFDRDGLVPCIAQNAETGEVLTLAYMNEESLRLTRESG
                     LVTYFSRSRQKIWQKGEESGNVQKVRQLRIDCDGDAIVALVEPSGPACHTGERTCFFR
                     DLETGANPEPVSGEALSVLARTIDSRLAEKPEGSYVVKLLEDPAFAGEKVMEEAEEVS
                     RAVREETDDRVANEAADVIFHLAALIRSRGITLDDVGEVLNGRRA"
     gene            4369..5895
                     /gene="trpE"
                     /locus_tag="PLJ64_08075"
     CDS             4369..5895
                     /gene="trpE"
                     /locus_tag="PLJ64_08075"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HNC93477.1"
                     /translation="MAVAPDLASRLTPDLETARALVPESNVVPVALRYTDDVETPVSA
                     LLKLRQGGPCFLLESAERGQVGRYSFVGVGPHRVIRWVAGELKEFPGEAMSGEGAPIA
                     TFPAADPYDGVKEYLSRYRVAETPGMPPFAGGAVGLFGYDLVRAIEPLGDPNPDPLGL
                     PDLALMITDVLLAFDHLHNELTIISCAWADEHENFDQAWEAAAERIGAVRESLRGPVP
                     DAGETGAVEAPEFTSNVTRDEFEATVARIVEYIHAGDAFQVVPSQRFSADQKAEALSI
                     YRGLRSINPSPYMYFFEFGDFQIAGASPEPLLKVNGDRVEIRPIAGTSPRGEDEEEDR
                     RLAEKMISDPKERSEHVMLVDLARNDIGREAGYGTVEVEELMVVETYSHVMHIVSRVS
                     GRLPEGRGALDVLRSALPAGTLSGAPKVRAMQIIDEMEKVKRCSYGGAVGYLSWNGDL
                     DTAIHIRTALIKDGQVHIQAGGGTVADAKPEYEYEESVNKAKAMFRAVEIACENPDWA
                     "
     gene            5895..6512
                     /locus_tag="PLJ64_08080"
     CDS             5895..6512
                     /locus_tag="PLJ64_08080"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HNC93478.1"
                     /translation="MRVLVIDNYDSFTYNLVQYLGELGAEVETVRNDVRTVDELLAEG
                     HDRLVVSPGPCTPDDAGISLEVIPAFAEAGVPVLGVCLGHQAMVQTFGGTVIQGEPIH
                     GKSAEIDHDGKSIYDGLPHPLTVGRYHSLIAGDVPDSLEVTSTFGDIVMGVRHRELPA
                     EGVQFHPESVLTPYGKRMIATFIGVELPLEQAAAGIPYNEGKPVA"
     gene            6505..7524
                     /gene="trpD"
                     /locus_tag="PLJ64_08085"
     CDS             6505..7524
                     /gene="trpD"
                     /locus_tag="PLJ64_08085"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HNC93479.1"
                     /translation="MPNDILTRAIDAAASGQHLTTDHASAVLTEIMEGRSDEVQTAAF
                     LIALRTKGETVPELIGLARTMRSLAEPVEVSGDLVDTAGTGGGPSTFNVSTTAALVAA
                     GAGCPVAKHGNRSSTSLSGSADLLEALGVDIEITPEKVAASIEETGFGFMFAPRHHKA
                     MAHVVPVRKALAVRTIFNFLGPLTNPAGARRQLMGVSDRRYQESIAEALAGLGTERAL
                     VVSGDDGIDEISITGPTRIIEVVDGGTSEWFVSPGDFELERAELGDVAGGTPDENAAA
                     TRAVLEGEKGPRRDLVVLNAAAAILVGGKAGDFAAGVVAAQEAIDSGAAAAVLARLVS
                     FTGQS"
     gene            7541..>8283
                     /gene="trpC"
                     /locus_tag="PLJ64_08090"
     CDS             7541..>8283
                     /gene="trpC"
                     /locus_tag="PLJ64_08090"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HNC93480.1"
                     /translation="MGILEQLISAASQGVKARSQQVPLADLEARLGERDHDRPFQEAL
                     TRPGMSLICEYKRKSPSAGEIAPGVELPDQVKAYEAGGAAALSVLTDETHFDGRLDDL
                     AAARAACSLPILRKDFVVDQYQLYEAAAGGADAVLLIAAVLEDDRLRDFQLKAAELDL
                     DCLVEVHDEEELERAVESGAEVIGINNRNLDTGVVDIQTTYDLITDVPTGTTVVSESG
                     ISNREELVELERVGVDAALIGESLMRSENP"
BASE COUNT         1490 a         2715 c         2738 g         1340 t
ORIGIN      
        1 ctgatcgaag gcggtcatga ggttgaagcc tccaccgtag gttcggtcac caacgcgatc
       61 cagtcttccc gccgcccggc cgaggtgctc gatgacgtgg tctggcacaa ccgcaatacg
      121 cgggtggtgc cgaaaaccgt caaccagaag gtctacgttg attcgattcg cgagaacacg
      181 gtcaccttcg gcatcggacc ggccggcacg ggcaagacat tcctcgcggt ggcgatggca
      241 gccgccgccc tgatcgaggg cgaggtccag cggatcatcc tgacccgacc ggcggtcgag
      301 gcgggggaaa gcctcggttt cctgccgggt gaccttcagg ccaaggtgga cccttacctg
      361 cggccgctct tcgacgccat ctacgacatg ctcgacccgg accgggtcaa cggctacttc
      421 gaccgaggcg tgatcgaggt cgccccgctc gccttcatgc gcggccgcac cctgaatgac
      481 gccttcgtga tcctcgacga ggcacagaac accacccccg aacagatgaa gatgttcctc
      541 acccggctcg gcttcaactc gaagatggtg gtgaccggtg acgtcaccca gatcgacctg
      601 cctcgtgaca gcaagtccgg gctggtcgtc gtgcgggaga tcctcgaggg gatcgaagac
      661 gtcaggttcg tgcgcttcgg cggcaaggac gtggtccggc accgtctggt ccagcggatc
      721 gtcgccgcat acggcgaaca cgccgaacgc gagaacggca agtgagcctt gaccttgatg
      781 acgtgccggc ccgtttccgg ccggccgtga ccgcgaccct cgaagcggca ggggtcggga
      841 atggccacct ggcggtggag gtcgtggatg aagccaggat ccggaaactg aaccgggacc
      901 accgcggcaa gaacaaaccg accgatgtcc tttccttccc ggtcgacggg gaagagggtc
      961 ccggcccggt tgagctgggg gacgtggtca tctgccccga attctgcgtc gacgaggtgg
     1021 aagcggtcgt ccacggagtg ctccacctct gcggttacga ccacgaaacc gatcaaggcg
     1081 agatgctcga cctccaggac cgcgtcgttt ccgggctggg gatcgaaccg aagatgaccg
     1141 tcgaggctga cgatgagtga aggcagccgc tcgggtttcg tcggactggc cggaaggccg
     1201 aacgtcggca agtcgaccct ggtcaacgcg atcgtcgggg ccaaggtcgc gatctcctcg
     1261 gtccggcccc agaccacgcg ccgggcgatc cggggagtgg cgaccgacct cgaggccggt
     1321 acccagctgg ttctggtcga tctgcccggg gtccagcggc cccgggacga gttgaccagc
     1381 cgcatgcaga agacggtcga aggcgaactg gccggggccg acgtcgccct gctggtgatc
     1441 aatggcgagg aaggcgtcgg gccgggcgac cggttcatcg ccgaaacgct gctcggggcc
     1501 cgggccaaag ccggcggctt gccaatcatc tgcgccgtca acaaggccga ccggctcggc
     1561 cggaacatcg tgccggtcct ggtcgccgcc gcggaactcg ccggtgtcga cgaggttttc
     1621 ccggtcagtg ccctgaccgg ccagggcgtg ccggaactcg cctcgtacct ggcctcgatc
     1681 gtccccgagg ggccttacct ctacccgccg gaggatcaca ccgatcagcc ccgggaactg
     1741 cttctggccg aactgatccg gggccaggcc ctgatccgta cccgggacga gattccccat
     1801 tcggttgagg tgcgggtgac cgggatcgag cagcgcgaag acggcctctg gctggtcgac
     1861 gccgagatct gggttgaatc ggactcccag aagcggatcc tgatcggcaa gaagggcttc
     1921 aaggtcggag agatcggcac cggggcccgc aaggacatcg aaggggaact cggcggcaag
     1981 gtccacctcg acctccaggt caaggtcaag cgaaagtggc gccgcgacga agacatgctg
     2041 gaccgcctcg gcattgatta gcccgggtgg cgccaggaac caccagccgc tgaagtgggg
     2101 tggtgttggc ttttcgagat cccgttttct gccatatagc gcacccaact ccgatctcga
     2161 tcgatatccg gccggatccg ggataggcgc cgcaaaaata ggcccatcaa gggcctgaat
     2221 gtgcggcacc tcccgaaacc agagcctgaa cccggccctg gaggtacggc ggggaagctc
     2281 ccccaccccg cggcgtaacc accgggcgaa tcaggctaga tgtcgcccga gaacgtgtcg
     2341 cagctggccg gatctcccga ctcgaagccg gtcttgaacc atctgtggcg ctgggcggag
     2401 gttccgtggg tgaacgagtc ggggttgacg ctgccgccgc cgcgggactg gaggcggtcg
     2461 tcaccgaccg cttcggaggc gccgatcgcc tcgtccaggt cgccggcctc gagctggtcg
     2521 aagacggttg acgcccagac tccggcgagg cagtcggcct gaagctcgtt acggaccgag
     2581 atctcgttgc gtgactcgga ttgctcacgc ttctcacggg acacctcgtc ggagatgccg
     2641 cccatgttct ggacgtgatg ccccatctcg tgagcgatca cgtaggccca ggcgaagtcg
     2701 ccgtcggccc cgagctggtt cttcatgtcc ttgtagaagc tcagatccag gtagacccgg
     2761 ttgtcggccg ggcagtagaa ggggccgacc gccgaagtgg cgttgccgca gccaccggtg
     2821 ctgaccgcgc ccgagtagag caccagctgc gcgtcctgcc agcgccggcc gtcccgggtg
     2881 aaggccttgc gccagacctg ctgggcgtcg gtgaagacgt aggccgagaa atccttgagg
     2941 tcggcatccg ggtcctcgcc ggcggggatc ggggagggat tctccgcgcc gggcacggcg
     3001 cccattcccg ggaacatcga tcccatgtcg aagccggtgc cgccaccgga gccgcccccg
     3061 ccgagaacct gggtgccgat cacgatgatc acgatgatca cgccgaccag gccgccgatc
     3121 ttgcccttgc cgcccggcag ctgcataccg ccaccgccca tgccaccgat gccgccgaag
     3181 ccaccaccgc cgcctccgcc gcccctcttc gcgccgcgga tatcgatcac gttccggttg
     3241 cctgaacggc ctcgcttcca cttcatggcc tgaaccctag acggcaagcc ggcatcgtgc
     3301 gaccggatcc cggtcaggct ttcgtaggat tgggccatgc tgcgaaaagt cgcaatctgc
     3361 gccctgatcg gatcggcaac gctggtctcc gcctgcggcg gttccgatga ctcgacttcg
     3421 agcgaggaca gtcaggcgat caccgaactc gtcgccaagc tgaatcaggc gacccaggaa
     3481 aaggatgcct cggccttctg cctgatcatg cagcccagtg cggtcgagca gacctttcac
     3541 gacatcgacc gttgcgtaag tgaaacgacc ccgatcctca aggcggccgg tgaccagggt
     3601 gtcctcaaga tcgacacgat cgaagtgaat ggcgacatcg ccgcggtcac gttccagggc
     3661 ggcaccagcc gcgaagcgaa gttcgtcaag gaagacggcc agtggtacgt gccgctcagc
     3721 cagagtgaga tcacgaccaa ccagggctcg aacggaagcg aaggctgatg ccggaagtcg
     3781 aagtgaagtt tgaccgggac gggctggtcc cctgcatcgc ccagaacgcg gagacgggcg
     3841 aggtgctgac cctcgcctac atgaacgagg aatccctgag gctgacccgc gagtccggtc
     3901 tggttaccta cttcagccgt tcgcgccaga agatctggca gaagggcgag gaatccggca
     3961 acgtgcagaa agtccgccaa ctccggatcg actgcgacgg cgacgcgatc gtcgccctgg
     4021 tcgaaccgtc cggccccgcc tgccacaccg gtgagcgcac ctgctttttc cgcgacctcg
     4081 aaaccggcgc gaaccccgaa ccggtcagtg gcgaagccct ttcggtgctg gcccgaacga
     4141 ttgattcccg cctcgccgag aagccggagg gaagctacgt ggtgaagctg ctcgaggacc
     4201 ccgccttcgc cggcgagaag gtgatggagg aagccgaaga ggtgagccgg gccgtccgtg
     4261 aggaaaccga cgaccgggtg gccaacgagg cggccgacgt gatctttcac ctggcggcgc
     4321 tgatccgatc gcgcggcatc accctcgatg acgtggggga ggttctgaat ggccgtcgcg
     4381 cctgacctcg cttcgcggct caccccggat cttgagaccg cccgggcgct ggttccggaa
     4441 tcaaacgtgg tgccggtcgc cctgcggtac acggacgatg tcgagacacc ggtttcagcc
     4501 ctgctcaagc tgaggcaggg tggcccctgc ttcctgctcg aatcggccga gcgcggtcag
     4561 gtcgggcgct actcgttcgt cggggtcggt ccccatcggg tgatccgctg ggtggccggc
     4621 gagttgaagg agttcccggg cgaggcgatg tccggcgagg gcgcgccgat cgcgacgttt
     4681 ccggccgcgg acccctatga cggggtcaag gaatacctga gccggtaccg ggtcgccgag
     4741 accccgggaa tgccgccatt tgccggtggc gcggtcggcc tcttcggcta tgacctggtc
     4801 cgcgcgatcg aacccctcgg tgatccgaac ccggacccgc tgggccttcc cgatctcgcc
     4861 ctgatgatca ccgacgtcct gctcgccttc gaccatctcc acaacgagtt gaccatcatc
     4921 agctgcgcct gggccgacga gcatgagaac ttcgaccagg cctgggaagc agccgccgaa
     4981 cggatcgggg cggtcaggga aagcctgcgc ggaccggtgc ccgacgcggg cgagaccggc
     5041 gcggttgaag ctcccgagtt cacttccaac gtgacgcggg acgagttcga ggcaacggtc
     5101 gcccggatcg tcgagtacat ccacgccggc gacgccttcc aggtggtgcc ctcacagagg
     5161 ttcagcgccg atcagaaggc cgaagccctc tcgatatacc gggggcttcg atcgatcaac
     5221 ccctcgccgt acatgtactt cttcgagttc ggtgatttcc agatcgccgg agcctcaccc
     5281 gagccgttgc tcaaggtgaa cggagaccgc gtcgagatcc ggcctatcgc cggcacctcg
     5341 ccgcgcgggg aggacgagga agaggatcgc cgcctcgccg agaagatgat ctccgacccg
     5401 aaggagcgat ccgagcacgt gatgctggtt gacctcgccc gcaacgacat cggccgcgaa
     5461 gccgggtacg gcacggtcga ggtggaagag ctgatggtgg tcgagaccta ttcgcatgtg
     5521 atgcacatcg tcagccgggt ctcgggcagg ctgccggagg gccgcggcgc cctcgacgtg
     5581 ctccgttccg ccctccccgc cggcactctt tccggcgcgc cgaaggtccg ggcgatgcag
     5641 atcatcgacg agatggagaa ggtcaaacgc tgctcctacg gcggcgccgt cggctacctc
     5701 tcctggaacg gtgacctcga caccgcaatc cacattcgca ccgccctgat caaggacggc
     5761 caggttcaca tccaggccgg gggcggcacg gtcgccgacg cgaagcccga gtacgagtac
     5821 gaagagtcgg tcaacaaggc gaaggcgatg ttccgcgcgg tcgagatcgc ctgcgaaaac
     5881 ccggactggg cctgatgcga gtactggtaa tcgacaacta cgactccttc acctacaacc
     5941 tggtccagta cctcggtgag ctcggggcgg aagtggaaac ggttcgcaat gatgtccgga
     6001 cggtcgacga gctgctggcc gaaggccacg accggctggt cgtatcaccg ggtccctgca
     6061 ccccggatga cgccggaatc tcgctcgaag tgattccagc cttcgccgag gccggcgttc
     6121 ccgtgctcgg ggtctgcctc ggccaccagg cgatggtgca gaccttcggg ggcacggtca
     6181 tccagggcga gccgatccac ggcaaaagcg ccgaaatcga tcacgacggc aagtcgatct
     6241 atgacgggct gccccacccg ctcacggtcg gtcgctacca ctcgctgatc gccggcgatg
     6301 ttcccgactc gctcgaggtg acttcgacct ttggagacat cgtgatgggc gtccgccaca
     6361 gggaacttcc ggccgaaggg gtccagtttc atcccgaatc ggtcctgacg ccctacggga
     6421 agcggatgat cgccaccttc atcggagtcg agcttccgct ggagcaggcc gccgccggaa
     6481 ttccgtacaa cgaagggaaa ccagttgcct aatgacatcc tgaccagggc gatcgacgcg
     6541 gctgcttcag gccagcacct gacgacagac catgcctcgg ctgtgctcac cgagatcatg
     6601 gaaggccgct ccgacgaggt gcagaccgct gccttcctga tcgccctcag aaccaagggc
     6661 gagactgttc ccgagttgat cgggctggcc cggaccatgc ggtccctggc cgagccggtc
     6721 gaggtttccg gtgatctggt ggacacggcc ggaaccggag gaggcccctc gactttcaac
     6781 gtctccacga ccgccgccct ggttgcggcc ggggccggct gtccggtggc caagcacggc
     6841 aatcgttcca gcaccagcct cagcggttcg gctgacctgc tcgaggcact tggcgtcgac
     6901 atcgagatca cgccggaaaa ggtggcggca tcgatcgagg agaccggctt cggtttcatg
     6961 ttcgcgccca gacaccacaa ggcgatggcg catgtggtgc cggtccgcaa ggcgctggcg
     7021 gtgcgaacga tcttcaactt cctgggacca ctgaccaatc ctgccggtgc caggcgccag
     7081 ttgatgggcg tctcggatcg ccgctaccag gagtcgatcg ccgaggcgct ggccggtctc
     7141 ggcaccgaac gggctctggt cgtttccggc gacgacggca tcgacgagat ctcgatcacc
     7201 ggccccaccc ggatcatcga ggtcgttgac ggcggcacca gcgagtggtt tgtctcaccc
     7261 ggcgatttcg aacttgagcg ggccgagctc ggtgatgtcg cgggcggcac cccggatgaa
     7321 aacgcggccg ccacgcgggc agtgctggaa ggtgaaaagg gtccgaggcg tgacctcgtg
     7381 gtcctcaacg cggcggcggc gatcctggtt ggcggcaagg ccggtgattt cgccgccggc
     7441 gtggttgcgg cacaggaagc gatcgactcc ggggcggccg ctgccgtttt ggcccggctg
     7501 gtcagtttca ccggtcagtc ctagtaggct ctgaagcgaa ttgggaatac tcgagcaact
     7561 gatttcggcc gcgagccagg gcgtcaaggc ccgcagccag caggtgcccc ttgctgacct
     7621 ggaggcaagg ttgggcgagc gtgaccatga ccgcccgttc caggaagcgc tgacccgtcc
     7681 cggcatgtcg ctgatctgcg agtacaagcg caagtcaccg agcgccgggg agatcgcccc
     7741 cggggtcgag cttcccgatc aggtcaaggc ctatgaggcc ggtggcgccg ccgctctgtc
     7801 cgtgctgacc gacgagaccc acttcgacgg ccggctcgat gatctcgccg cggcccgcgc
     7861 cgcctgcagc ctgccgatcc tccgcaagga cttcgtcgtc gaccagtacc agctctacga
     7921 agccgcggcc ggcggcgccg acgcggtcct gctgatcgct gccgtgctcg aagacgatcg
     7981 cctgcgcgat ttccagctca aagcggccga gctcgatctc gattgcctgg tcgaggtcca
     8041 tgacgaggaa gagctcgaac gggcggttga atccggggcc gaggtgatcg gaatcaacaa
     8101 ccgcaacctc gacaccggag tggtcgacat ccagaccacc tacgacctga tcaccgatgt
     8161 tccgaccgga accacggtgg tttccgaatc cgggatctcc aatcgcgagg aactggtcga
     8221 actcgaaagg gtcggtgtcg acgccgccct gatcggcgag tccctcatgc gctcggagaa
     8281 ccc
//
LOCUS       DAOQKN010000109         8275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_4184_length_8275_cov_3.941241, whole genome shotgun sequence.
ACCESSION   DAOQKN010000109 DAOQKN010000000
VERSION     DAOQKN010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 8275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8275
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4184_length_8275_cov_3.941241"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="PLJ64_08095"
     CDS             <1..654
                     /locus_tag="PLJ64_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572566.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNC93481.1"
                     /translation="SRLEGADGILIPGGFGERGVEGKIEAARYARENKIPYLGICLGM
                     QIAVIEFARHIAGMDGANSAEFDPETPFPVVDLLPEQKEVSDMGGTMRLGADPVKLHE
                     GTRALEIFGEPVIYKRHRHRYEVNNLLRTRLEDQGLICSGTSPDERLVEIIELDDHPF
                     YVASQFHPEFNSRPNRPEPLFREFMKAVTKVAADRPEELQDVDAEGRPATGVETQEA"
     gene            657..1187
                     /locus_tag="PLJ64_08100"
     CDS             657..1187
                     /locus_tag="PLJ64_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93482.1"
                     /translation="MPFLFWILVALIGAGIHLARHKDQRGAAEVSKVLLMYWLALAMG
                     LGSIFGAAFHVFDAKETAEQIGFVPWNPTGTGAFQFENAMGDLAIGVAALLCLWIRDA
                     KFWLAVIIVATIQFWGDAYGHIHQMMEYDNHDPDNTGFVLWLDIANPVVVILLYLIAF
                     QPPLKDNGNPPASSVH"
     gene            1247..2377
                     /locus_tag="PLJ64_08105"
     CDS             1247..2377
                     /locus_tag="PLJ64_08105"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNC93483.1"
                     /translation="MSRFVRLCETASPTGEERAIADLVRSELELLGLEVHEDDAAGPA
                     GAAAGNLLTRIPGRPREDGTDPGYLMFCAHLDTVPHDDRIEVVLDEHGVYRSAGETIL
                     GADNKAAVAVLIEMAARSTVDAPPVGLELLFTVAEEQGLRGAAAFDQSQLRSTVGFVL
                     DHATDIGEVITAAPTHIGIRAEFHGVEAHAGLIPESGRSAIAAAAGAIAEMKLGRLDM
                     ETTANVGKIAGGTSGNVIPGFCALTGEARSVDPVRVTEVIGEMTDAMIWAASESGIEI
                     DVATERHFAGYRVEDDSRALDLAESALASRGHQPQRITTGGGSDANVFRERGMDCLLL
                     ANGTYANHTSDENVPRENLSEMLEICLAIANCAGDDPARVEA"
     gene            2377..3378
                     /locus_tag="PLJ64_08110"
     CDS             2377..3378
                     /locus_tag="PLJ64_08110"
                     /inference="COORDINATES: protein motif:HMM:NF024384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HNC93484.1"
                     /translation="MLKLRRATVVSVEPLEVRLEVSDQPRPAWADESMVGPCEAGDEV
                     IVNVEALDLGLGSGGFDVIHVNLTRGLSGEGSATEHVMKLNYTSLQHPVGTIEREEGT
                     GRAPVVPVLAIGLHGHLAPAAWAAAEAGAEAIGYIQTGGGALPGSLSRDVSELLDRGL
                     LAGHVTAGPAYGGGGEAISLVGAIDAASSLGWGGVIAGPGPGILGSGTAFGHGGMAAL
                     DVIHASLALGAPVLLSPRLSAGDPRPRHRGLSHHSRSVLDLALGPVEFPVPAGFLDLI
                     PEGTPGAKSVEVNLEGYGESGLPQKTMGRDLEEDRPFFAGPLAAGTRLGQLIAEPAT"
     gene            3468..4433
                     /gene="xerD"
                     /locus_tag="PLJ64_08115"
     CDS             3468..4433
                     /gene="xerD"
                     /locus_tag="PLJ64_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HNC93485.1"
                     /translation="MPPATVTSVPAPEVLEGARFEGLLLEFLAYLELERGLSRNTLNA
                     YRTDLLQYGDYLRAKGIDPLEIDAAGMSEYVADLIEGKGSGHRLAASTVHRKTAALRS
                     FYKHLRLEDLVGDDPTAGLSTPRRSKKLPNVLNQAEVTRLLAVPAGDSPQAYRDRAIL
                     EVMYACGLRASEVIDLEMTDVDTYEGMLRARGKGSKERIVPLGRQAIYALEGYLLSGR
                     PDLVKGRSEKALFLNFRGRRLTRQGLYKIVQGHAKSAGLEDRMSPHTLRHSFATHLLA
                     GGCDLRAVQEMLGHADVATTQMYTHLTGQQLRDVYYAAHPRAQAN"
     gene            4595..5482
                     /gene="mutM"
                     /locus_tag="PLJ64_08120"
     CDS             4595..5482
                     /gene="mutM"
                     /locus_tag="PLJ64_08120"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HNC93486.1"
                     /translation="MPELPEVETIRRQLEPDLNGRVIETVEVLDPYFTKPDLPGNFER
                     AVNGREIVDVGRRGKYLLLELDDGLTVVIHLRMTGSLVLLAPDGSENPGDARIYEADR
                     SERHLKARFTLDDGGELRFSDPRRFGRGFVANGEDLETYFASRLGVEPLSDDFTVEAV
                     AAMSAGRTGPLKSFLLDQKGVVGVGNIYADEALFRAGLHPLSPGGSMKPEHHLALRDG
                     VVEALQRGLDLGGASIDDYRDAKGEKGGMQKEFLVHRREGESCVTCGGEIRRIVVSGR
                     STYFCPTCQKRLRRKSKRK"
     gene            5529..6269
                     /gene="recO"
                     /locus_tag="PLJ64_08125"
     CDS             5529..6269
                     /gene="recO"
                     /locus_tag="PLJ64_08125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNC93487.1"
                     /translation="MSRTFKTEAVVLRTIRYGEADRILHLYTREHGRLGAIAKGVRKP
                     RSRFGGRLEPFFRLNLVLHRSRGDLHTVTSAETIEGYPELRASGRGITAAADACGGVL
                     RLLDGIEEPNEPAFNLLCRYLELIDADPEGPAASAAGMVTFRLKLALAAGFAPELSGC
                     SRCGATDHMTAFSGAAGGVVCPTCEAGSFPFTEEAHTFMVSSLGMPMAEAPAGSPAAV
                     RQAGRAIGETLEHHAHVKLPRPEERLAA"
     gene            6266..7300
                     /locus_tag="PLJ64_08130"
     CDS             6266..7300
                     /locus_tag="PLJ64_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HNC93488.1"
                     /translation="MNGSNRKGDGPDSEFELMTRQREEKYLGPRATRSYPADRPEPEP
                     DSQWRTPFQRDRDRIVHSKSFRRLMHKTQVFIAPEGDHYRTRLTHTLEATGIARTVAR
                     ALRLNEDLTEAIGLAHDLGHPPFGHIGEQALDEASRSHGGQGFKHNENSLRVVDVLER
                     EGRGLNLNQPVREGILKHTGPVKSESLEGQIVRLVDRVAYINHDIDDALRAGILSSED
                     LPNEEIAMLGPTGASRIDSLVKDIVNQSRDAGEIVQSEHMGMAMLRLRKFMFDNVYLG
                     GEARKDHDRVHGVIRALFEHYLERIEEVPVLDPGADPIQRVTDLLAGMTDRYCIAIFT
                     SIYVPEGSRF"
     gene            7320..>8275
                     /gene="dnaG"
                     /locus_tag="PLJ64_08135"
     CDS             7320..>8275
                     /gene="dnaG"
                     /locus_tag="PLJ64_08135"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNC93489.1"
                     /translation="MAKITDDTKEQVRAAADMVEIVSAHTDLKRAGGGRYMGLCPFHE
                     ERSPSFSVNNNDKLYHCFGCGVGGDIFDFVMSIENVDFPTALEQLADRYGVELKREDE
                     DPRAAQRRQEMARMTELMDRAGAFYSNFFQTADEAGKAREYLAGRGLGDEILTQFGVG
                     YAPSAWDTLLVRSQRAGFKVNELERAGLAQKGRNGGYYDRFRERITFPIRDARGRAVG
                     FGARAMRDEQGAKYINTAENALFHKSEILYGIDRARPSMAKADRAILVEGYTDVIALH
                     QAGLTEAAGIMGTALTKPQVAQLSAIVNTVVLALDADAADQKA"
BASE COUNT         1516 a         2594 c         2725 g         1440 t
ORIGIN      
        1 tcgaggctgg agggggccga cgggatcctg atcccgggcg gattcggcga gcgcggcgtg
       61 gaaggcaaaa tcgaggctgc ccgatacgca cgcgagaaca agatccccta cctgggcatc
      121 tgtctcggaa tgcagatcgc ggtgatcgag tttgcccggc atatcgccgg catggacggc
      181 gccaactcgg ccgagttcga tccggaaact cccttcccgg tggtcgacct gctgccggaa
      241 cagaaggagg tctcggacat gggcgggacg atgaggctgg gggcggatcc ggtcaagctc
      301 cacgagggca cccgggccct ggagatattc ggcgaacctg tgatctacaa gcgccaccgt
      361 catcgctacg aggtcaacaa cctccttcgc acccgtcttg aggaccaggg gttgatttgc
      421 tccggcacct cgccggacga gcgtctggtc gagattatcg aactcgacga ccatcctttt
      481 tacgttgcct cacagtttca ccccgagttc aactcacgtc cgaaccggcc ggaacccctt
      541 ttccgggagt tcatgaaggc ggtaaccaaa gtcgctgcgg atcgcccgga agagcttcag
      601 gatgtggacg ccgagggtcg cccggcgacc ggagtcgaga cccaggaggc ttaggcatgc
      661 cgttcctgtt ctggattctc gtcgccctga ttggcgccgg gattcacctc gcgcgccaca
      721 aggaccagcg gggagctgcc gaagtctcga aggtgttgct gatgtactgg cttgccctgg
      781 cgatgggcct tggttccatc ttcggcgctg ccttccatgt cttcgacgcg aaagagactg
      841 ccgagcagat cggtttcgtg ccttggaacc cgaccggcac cggagccttc cagttcgaga
      901 atgcgatggg ggatctcgcg atcggggtcg ccgccctgct ctgcctgtgg attcgtgatg
      961 cgaagttctg gctggccgtg atcatcgtcg ccacgatcca gttctggggt gacgcctacg
     1021 gtcacatcca ccagatgatg gagtacgaca accacgaccc cgacaacacc ggcttcgtgc
     1081 tctggctcga catcgcaaat ccggtggttg tgatcctgct gtatctgatc gccttccagc
     1141 ccccgctaaa ggacaacgga aacccgccgg cgagcagcgt tcactgacgg gctggccggc
     1201 ttcggtcccg gttgctgcgt tcgcgtcgtg agatgattcg gctcgagtga gccgttttgt
     1261 ccgactctgc gaaacagcca gcccgaccgg ggaagagcgg gcgattgccg atctggttcg
     1321 cagcgaactc gaactgctcg gactcgaggt tcatgaagat gatgcggccg gtccggccgg
     1381 tgccgctgcc ggcaacctgt tgacccggat tcccggacgg ccgcgtgagg acgggaccga
     1441 ccccggctac ctgatgttct gcgcccacct cgacacggtg ccacatgacg atcggatcga
     1501 ggtcgtgcta gatgaacacg gggtgtaccg ctcggctggg gaaacgattc tcggcgccga
     1561 caacaaggcg gcggtcgcgg tgctgatcga gatggccgcc cggtccacgg tcgatgcgcc
     1621 gccggtcggt ctcgaattgc ttttcacggt ggccgaggag cagggtctgc ggggggccgc
     1681 cgcgttcgac cagtcgcaac ttcggtccac ggtcggcttc gtcctcgatc acgccaccga
     1741 tatcggggaa gtgatcaccg cagcaccgac acatatcggt atccgggccg agtttcacgg
     1801 ggtcgaggca catgcggggc tgatccccga atcgggacgg tccgcgatcg cggcggcggc
     1861 cggggcaatc gctgagatga aactcggtcg cctcgacatg gaaacgaccg ccaacgtcgg
     1921 aaagatcgcc ggtggcacct cgggcaacgt gattccaggt ttctgcgccc tgactggcga
     1981 agcccgatcg gttgacccgg tgcgggtcac cgaggtgatc ggagagatga ccgacgcaat
     2041 gatctgggcc gcctccgaga gcgggattga aatcgatgtg gcgacggaga ggcacttcgc
     2101 cggctaccgg gtcgaggatg attcaagggc tttggacctg gctgaatccg cgcttgccag
     2161 tcgcggccat caaccgcagc ggatcacgac cggtggcggc tcggacgcga acgtgttccg
     2221 ggagcgcggc atggactgcc tgctgctcgc caacggcaca tacgccaacc acacctctga
     2281 cgagaacgtt ccccgtgaga acctttccga aatgctggag atctgcctgg ccatagcgaa
     2341 ctgcgcgggt gacgatcctg cccgggtgga agcctgatgc tgaagctgcg ccgggccact
     2401 gtggtttccg tagagccact tgaggtcagg ctcgaagtct ctgaccagcc tcgcccggcc
     2461 tgggccgatg aatcgatggt cggtccctgt gaggccgggg acgaggtgat cgtcaacgtc
     2521 gaggcacttg atctcggatt gggatctggt ggattcgacg tgatccacgt gaatctgacc
     2581 cggggcctga gcggagaagg ttcggccacc gagcacgtga tgaagctgaa ctacacctcg
     2641 ctccagcatc cggtcggcac gatcgagcgc gaggaaggga ccgggcgggc cccggtggtt
     2701 ccggttttgg cgatcggtct gcatggtcac ctggctccgg cggcctgggc ggctgcggag
     2761 gccggggcgg aagcgatcgg gtacatccag accggcggcg gggcgctgcc cgggtccctt
     2821 tcgcgggacg tgtcggaact gctcgaccgg ggccttctgg ccgggcatgt gaccgccgga
     2881 ccggcctacg gcgggggagg agaggcaatc tccctggtcg gggcgatcga cgccgcctcc
     2941 tcgctcggat ggggcggggt gatagccggg ccggggcccg ggatcctcgg ctccggtacc
     3001 gctttcggcc atggcggaat ggcggcgctt gacgtgattc acgccagtct ggcactggga
     3061 gctccagtgc tgctttcacc gagactctcg gcgggtgacc cgcggccgcg acatcggggg
     3121 ctaagccacc acagccggtc cgttctggat ctcgctctcg gtccggtgga gtttccggtc
     3181 ccggccgggt tcctcgacct gattcccgag gggactcccg gagcgaagtc ggtcgaagtc
     3241 aatcttgagg ggtatggcga gtcgggactg ccccaaaaaa cgatgggccg cgatctggaa
     3301 gaagacaggc ccttcttcgc cggtccgctc gccgctggca cccgactcgg ccagctgatc
     3361 gccgaaccgg caacctgatc gccgcctgcc cgcgattcgc ctggttacag ggtcggaggt
     3421 ttcgttgcgg ggcaagggat gaagcgggtg ggggagaaag cgctttcatg cccccggcaa
     3481 ccgtcacgtc cgtccctgct ccggaagtac tcgaaggcgc ccgtttcgaa ggcctgctgc
     3541 tcgagttcct cgcctatctt gaactggagc ggggtctttc gcgcaacacc ctgaatgcct
     3601 accgcaccga cctgctgcag tacggtgact atctgcgggc aaaagggatc gatccgcttg
     3661 agatcgacgc agccggaatg tccgaatacg tggccgacct gatcgagggc aaaggttccg
     3721 gccaccgact cgctgcttcg accgttcatc gcaagaccgc cgccctgcgt tcgttttaca
     3781 agcatctgcg actcgaggat ctggtcgggg acgacccgac cgccgggctg agcacacctc
     3841 gtcgctcgaa gaagctgccc aatgtcctca accaggccga ggtgacacga cttctcgcgg
     3901 ttccggcagg agattctccg caggcttacc gggatcgggc gatcctcgaa gttatgtacg
     3961 cctgcggact gcgggcctcc gaggtgatcg acctcgagat gaccgacgtc gacacctacg
     4021 aggggatgct gcgggcgagg ggcaagggca gcaaggaaag aattgtgccg cttggtcgac
     4081 aggcgatcta cgctctggag gggtacctcc tttcgggtcg gccggatctg gtcaaggggc
     4141 gatccgaaaa ggcgcttttt ctcaactttc ggggccgccg cctgaccagg cagggtctct
     4201 acaagatcgt gcaggggcat gcgaaatcgg ctggcctcga agaccgaatg agccctcaca
     4261 ccctgcgcca ctccttcgcc acccacctgc tcgccggtgg ctgtgacctg agggcggtac
     4321 aggagatgct cggccacgcc gatgttgcca cgacccagat gtacacccac ttgaccggcc
     4381 agcagctccg cgacgtctat tacgccgccc accccagagc ccaggctaac tgagaagcca
     4441 aatccccggt gggtcggatt tccggtccac acgccgagaa tccgacccac ctggtttacg
     4501 aaccactcac cggcgcggta gtgggtcgga aaaccggtgc ctgtgccgga aatccgaccc
     4561 actgccgagg ggagggctct gatacaaccg gcgggtgccc gagcttcctg aagtcgagac
     4621 cattcgtcgc cagcttgagc ctgatctgaa cgggcgggtg atcgaaacgg ttgaggtgct
     4681 cgacccctac ttcaccaagc ccgatctgcc cgggaacttc gagcgggccg tgaacggacg
     4741 ggagatcgtg gatgtggggc ggcgcggcaa atacctgctg ctcgaactcg acgacggctt
     4801 gaccgtggtc atccatctgc gaatgaccgg ctccctggtc ttgctggcac cggacggcag
     4861 cgagaacccg ggtgacgcca ggatctacga ggccgaccgg agcgagaggc acctcaaggc
     4921 ccgcttcacc ctggatgacg ggggcgagct tcgcttttcg gatccgcgac gcttcggccg
     4981 gggcttcgtg gcgaatggcg aggacctgga gacatatttc gcatcccggc ttggagtcga
     5041 accgctttcc gacgatttca cggtcgaagc cgtggcggcg atgtccgcag gccggaccgg
     5101 accgctgaag tccttcctgc tcgatcagaa gggcgtggtc ggagtcggca acatctacgc
     5161 cgacgaggcc ctgttccggg ccggactcca tccgctctcg ccgggcggct cgatgaaacc
     5221 ggaacaccac ctggccctcc gggacggagt ggtcgaggcg ctccagcgcg gtctggacct
     5281 cggcggcgcc tcgatcgatg actaccggga cgccaagggc gagaagggcg gaatgcagaa
     5341 ggagttcctg gtccaccgcc gcgagggcga gagttgcgtg acctgtggcg gtgagatccg
     5401 gcgcatcgtg gtcagcggcc gctcgactta tttctgtccg acttgccaga aaaggctccg
     5461 acggaagtcg aagcgcaaat agacgtcagt gggcgtccgg tccgggcgac atcattaacc
     5521 ggttggacat gtcgcgaacc ttcaagacag aagcagtggt gctcaggacg atcaggtatg
     5581 gcgaggccga ccgcatcctc cacctttaca cgcgcgagca cggtcgactt ggtgccattg
     5641 ccaagggcgt ccgcaaaccg aggtccaggt tcggtggccg cctcgagccg ttcttccggc
     5701 tcaacctcgt ccttcaccgc agccggggtg atctccatac cgtgacttcg gccgagacga
     5761 tcgagggcta tcccgagctg agggcgagcg gccgggggat caccgctgcg gcggacgcct
     5821 gcgggggagt gctgcgtctg cttgacggga tcgaggaacc gaacgaaccg gccttcaacc
     5881 tgctttgccg ctatctggaa ctgatcgatg ccgaccccga aggtccagct gcctcggccg
     5941 ccggcatggt taccttccgc ctgaaactgg ctttggccgc cggcttcgcc ccggaacttt
     6001 ccggctgctc gcgctgcggg gcgaccgatc acatgaccgc tttttcgggt gccgcgggcg
     6061 gggtcgtctg cccgacctgc gaggccggtt catttccctt caccgaggag gcgcatacct
     6121 tcatggtcag ctcgcttggc atgccaatgg ccgaagcgcc ggctggcagt cctgccgccg
     6181 tgcgccaggc cggccgggcg attggcgaga ccctggagca tcacgctcat gtgaagctcc
     6241 cgaggccgga agagaggctg gccgcttgaa cggatctaac cgaaaaggtg acggtccgga
     6301 cagcgagttc gagctgatga cccggcagcg tgaggaaaag tatctcggtc cgcgggcgac
     6361 ccgctcctac ccggccgacc ggcctgaacc ggagccggac agccagtggc ggacgccttt
     6421 ccagcgcgac cgtgaccgca tcgtccacag caagtcgttc cggcggctga tgcacaagac
     6481 ccaggttttc atcgcgcccg aaggcgacca ctatcggacc cggctcaccc acactctcga
     6541 ggcaaccggg atcgcccgca cagtggcccg ggccctgaga ctgaacgagg atctgaccga
     6601 ggcgatcggc ctggcgcatg accttggtca cccacccttc ggtcacatcg gcgaacaggc
     6661 actcgatgaa gcgagccgga gccacggcgg gcagggtttc aaacacaacg agaactcgct
     6721 gcgggtggtc gacgtgctcg aacgcgaagg ccgaggactg aacctgaacc agccggtgcg
     6781 cgagggaatt ctgaagcaca ctggaccggt caagagcgag tcccttgaag gccagatcgt
     6841 tcggctggtc gatcgtgtcg cctacatcaa ccacgacatc gacgacgctc tgcgggccgg
     6901 gatcctcagt tctgaagacc tcccgaacga ggagattgcg atgctcggcc cgaccggcgc
     6961 cagccggatc gattccctgg tcaaagacat cgtcaaccag tcccgggacg ccggcgaaat
     7021 tgttcagagc gagcacatgg gcatggcgat gctgcggctg cgcaagttca tgttcgacaa
     7081 cgtctatctc ggcggcgagg cccgcaagga tcacgaccgg gttcacgggg taatccgggc
     7141 cctcttcgaa cactatctgg agcggatcga ggaggtgccg gtgcttgatc caggggccga
     7201 tccgatccag cgggtcaccg acctgttggc cggaatgacc gaccgctact gcatcgccat
     7261 cttcacgtcg atctacgtgc cggaaggttc ccggttctga gttcgtagac tcgaccgcga
     7321 tggcgaagat caccgatgac accaaggagc aggttcgtgc cgccgcggac atggtggaaa
     7381 tcgtctccgc ccacacggac ctgaaaagag ccggcggcgg tcgctacatg gggctatgcc
     7441 cctttcacga agaacggtcg ccgtcgtttt cggtcaacaa caacgacaag ctctaccact
     7501 gcttcggttg cggagtcgga ggcgacattt tcgacttcgt gatgagcatc gagaacgtcg
     7561 actttccgac cgcgctcgag caactggccg accgctacgg agtcgagctc aaacgggaag
     7621 acgaggatcc gcgagcggca cagcgccgcc aagagatggc ccgaatgacc gaactgatgg
     7681 accgggccgg cgccttctac tcgaactttt tccaaaccgc agacgaggcc gggaaggcca
     7741 gggaatacct ggcgggtcgc ggacttggcg acgaaatcct cacacagttc ggggttggct
     7801 acgcgcccag cgcctgggac acccttctgg tcaggagtca gcgggccggc ttcaaggtga
     7861 atgaactcga acgggctggc ctagcccaga aaggaagaaa cggcggctat tacgaccgtt
     7921 ttcgcgaaag gatcaccttc ccgatccggg acgcccgcgg ccgggcggtc ggcttcggcg
     7981 cccgggcgat gcgtgacgag cagggcgcca agtacatcaa cactgccgag aacgcgctct
     8041 tccacaagag cgagatcctc tacggaattg atcgtgcccg tccgtcgatg gccaaggcgg
     8101 accgggcgat cctggtcgag ggctacaccg atgtgatcgc tctccatcag gccggattga
     8161 ccgaagcagc tggaatcatg ggcacggcgc tgaccaagcc gcaggtggcc caactctcgg
     8221 cgatcgtcaa cacggtcgtg ctcgctctcg acgcggatgc tgccgaccag aaggc
//
LOCUS       DAOQKN010000110         8262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_4194_length_8262_cov_5.120019, whole genome shotgun sequence.
ACCESSION   DAOQKN010000110 DAOQKN010000000
VERSION     DAOQKN010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 8262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8262
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4194_length_8262_cov_5.120019"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PLJ64_08140"
     CDS             complement(<1..214)
                     /locus_tag="PLJ64_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93490.1"
                     /translation="MTDTYIQSNQPEGVPALELTGERTLPDVPEENYWYRRHLVVYEW
                     IAERVRGLKVADLACGEGKAHDRFHLM"
     gene            246..1163
                     /locus_tag="PLJ64_08145"
     CDS             246..1163
                     /locus_tag="PLJ64_08145"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC93491.1"
                     /translation="MPASPTATPEHIKDVNTRYHDAAADSYDAKWGIDFGDIGQHQVA
                     LKLKKALGELPRTLGDTLEIGAGTGYFSLNLATQGLIESLTATDISPGMLKSLARTAG
                     SLNFPVETVVTEAEVLPFEDESFDTVIGHAVLHHIPDLDQAFSEFFRVLRPGGRIVFC
                     GEPSRYGDRLAAVPKRTGNLVAPAWRKLVGADTLEHTEEEKADDEHSLEPEVDVHAFV
                     PADLRAIPPRSGFVETRVSGEELVANVWGWGMRSMESTATPDSIPLRWRNFAFRSYIA
                     FQKVDNNLLEPHLPAELFYNLLLSARKPA"
     gene            complement(1347..2537)
                     /locus_tag="PLJ64_08150"
     CDS             complement(1347..2537)
                     /locus_tag="PLJ64_08150"
                     /inference="COORDINATES: protein motif:HMM:NF028011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate cyclase regulatory domain-containing
                     protein"
                     /protein_id="HNC93492.1"
                     /translation="MADYPDPDDYPSPDLRPFMAADVATRELPDFEAEGLLEGLEGDD
                     RAARIKLLTELYMQDGVELSTLKEEADANRLALLPSELVLGRARDKYTPAEIAELAGM
                     SVNDFQNLIAAVGLPRPKPDQRTFDDADLTAARRLKRFQEAGIPKENLLAVTRQVGSS
                     AARIAQSHRDLVASDIIEPGADEYEAAVQLRQASLQLLPLAEQAVDYALRSHFLDQLR
                     NQAMAMASPGFFDPKESTEVSICFADLVGFTRLGERSELEKVGTVAKLLESVAIEVTN
                     PEVRLVKTIGDAAMLVSEDGAALFDAAIRFIEVGNEAGEDLPALRVGVARGEAIKQVG
                     DWFGPPVNLASRITEAARPDSVLADKKAVDAAGEEEFSYSWVGQHKFKGLDRPVKLFR
                     VRRK"
     gene            complement(2530..4011)
                     /locus_tag="PLJ64_08155"
     CDS             complement(2530..4011)
                     /locus_tag="PLJ64_08155"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HNC93493.1"
                     /translation="MDGRGVVAAGHPLSAEAGARILREGGNAVDAAVAAALASFSLES
                     ALTGLGAGGFMIVGGPGIETAVIDFFVAAPGRGGHELTEELLPVPVHFDESTTQVFNA
                     GAASCGVPGTAAGLALALERYGSVPMAELTGPAAALAREGAPLNEQQAYVLRILEPIY
                     TATPEARAIYAPEGKILGQGDVFRYPDLGDALDRFGAEGAGALCSGEVPRLVEEYIVS
                     RGGTLSVADFEAYEPIVRAPVGARYRGRELLTNPPPSAGGILVAYSLALLERMEGSGP
                     EEIARAMAAANEARDDDFALSLGQEGFARRFLDDRLGSTTHLAAIDSDGLCASVTCSN
                     GTGSGMVVPGTGFHLNNMLGEEDLNPRGFHQSAPGERISSMMSPTLILEQGMVRAGLG
                     SAGSNRIRSAVLQTTLNLLAGVDPQAAVDAPRIHLEAGVLQAEPGVDEEALGRIEAAG
                     QEVFRWNQRNLFFGGVQIVTRDPETGRLDGAGDPRRGGAVAYG"
     gene            complement(4015..5517)
                     /gene="ftsZ"
                     /locus_tag="PLJ64_08160"
     CDS             complement(4015..5517)
                     /gene="ftsZ"
                     /locus_tag="PLJ64_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNC93494.1"
                     /translation="MREGPLADLFRSTSDEPSQDPPEFEPRTADDTGGPQGDPVEPAA
                     AASTPAPEPEVHVEPDPDAAGRFGRRDPLEGRQEEPAPATPAEPAPRAEDPGPLSAPD
                     PAAETSNVPDAQDRLKRIFSEAPQDRDDGPRYGRQEPSSTPGAGVPHNPVIRVIGVGG
                     GGVNAINRMIEAQIPGVEFMAVNTDLQSLQLCMADLTVHIGGDETRGLGAGADPSLGF
                     RAAFDEQDRIKRLLKGSDMVFIAAGAGGGTGTGAAPVIARLARDIGALTVGIVTKPFT
                     FEGSRRQKQADQGIEALAGEVDTLIVIPNDRLLEVLDQSTPMVDAFQVADDVLRQGVQ
                     GISDLVTLPSVINLDFADVRSIMSDAGRALLGIGMGSGPDRALIAAEKATSSPLLETP
                     MDGAKSILLSVTGGPDLSLIEVSEAARAVGEAAHPDANIIFGANIDDDLDDQVWITVV
                     ATRFDGKGAPARRVERSERDRRDRGGSTARPGRGESLPAVGGSDIPEFLS"
     gene            complement(5528..6664)
                     /locus_tag="PLJ64_08165"
     CDS             complement(5528..6664)
                     /locus_tag="PLJ64_08165"
                     /inference="COORDINATES: protein motif:HMM:NF012369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC93495.1"
                     /translation="MRILSRKNQEREKQGPEVETVPEVESAVPEPVAPAATATVEPNP
                     GPVEAAATPAEPATPPELAAPAEPATPAEPAAVPVEEELAEVEEAAAAEETEIRLKAA
                     DLLAPSRRDLSDHLKPVQPEQPVDMAEVISFANQKGGVAKTTSTLNLAVALSELGNRV
                     LCIDLDPQGNLTMSQGIDPDKCEKSMYDVLVDDLPISEVIAHREIDIAVASIDLAGAE
                     IAMSTKIGRERSLEKALKEVHSDYDYICIDTPPSLGLLTINALTASSKVIVPVQCEYL
                     SMRGLVQLQSTLKMIQENLNPNVHIEGILPTMFDSRTLHAREAVFILEENFGDLVFKS
                     RIRKAVKFAEAPVRGASVLKYDPESRAASYYRNLAKEVLSDGSS"
     gene            6719..7651
                     /locus_tag="PLJ64_08170"
     CDS             6719..7651
                     /locus_tag="PLJ64_08170"
                     /inference="COORDINATES: protein motif:HMM:NF014433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HNC93496.1"
                     /translation="MNAFIPDFLDKRLLFVTGKGGVGKTTVAMALGIRAAMEGKRTIV
                     CEVAATENASRLFAQSEVGFREVELANDLWAISIDPDESMREYVLLQLKVKAMRDLLF
                     RSRMFTYLAAATPGLNELVTIGKIWELAQFDRKVKHGRKYDLVIVDAPATGHGISFLQ
                     TPRTFASIARVGPIHSQALQLEALITDHEQTGTVIVSLPEEMPVNETARLESELSGDI
                     GIAIDRVVMNALYPETFPKGDVKRLGKLKKSDDAEVRAAGRAGASQATRAAAQREQLA
                     RLEEMVETEVTTLPFIFKPELDLKAARQLAEEIT"
     gene            7651..>8262
                     /locus_tag="PLJ64_08175"
     CDS             7651..>8262
                     /locus_tag="PLJ64_08175"
                     /inference="COORDINATES: protein motif:HMM:NF014433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HNC93497.1"
                     /translation="MASVEQIIDGREIVICAGSGGVGKTTTSAAIALGMAARGHKVCV
                     LTIDPAKRLADSLGLKELGNEASKVNPKLLKEQGVDPENGGELWAMMLDAKETFDALV
                     ARHAEDAEARDRVLNNRIYQQISGALAGSQEYMAMEKLFELHSEGRFDLLVLDPPPSR
                     NALDFLDAPQRLTQFIEGKSLQIFIRPTGLAARVASRGTGVALS"
BASE COUNT         1389 a         2775 c         2690 g         1408 t
ORIGIN      
        1 gcatcaggtg aaaacgatca tgcgctttac cctcgccgca ggccagatcg gcgaccttca
       61 ggccccggac ccgctccgcg atccactcgt agacgaccag gtgccgccgg taccagtagt
      121 tctcctccgg cacgtccggc agggtccgtt caccggtcaa ttcgagggcc ggcactcctt
      181 cgggctggtt gctctggatg tacgtgtcag tcaaggaaac cgattcttcc ctatctttct
      241 ccgccatgcc cgcttccccg accgcaacgc cggaacacat caaggacgta aatacccgct
      301 accacgacgc ggcggctgac tcctacgacg ccaagtgggg gatcgacttc ggcgatatcg
      361 ggcagcacca ggtcgccctg aagctgaaga aggcgctcgg cgaattgccg aggacgctcg
      421 gggacaccct cgaaatcggc gccggcaccg ggtacttctc gctcaacctg gcgacccagg
      481 ggctgatcga aagcctcacc gcgaccgaca tttcgccggg catgctgaag tccctggccc
      541 gcaccgccgg gtccctgaac ttcccggtcg aaaccgtggt caccgaggcg gaggtgctcc
      601 ccttcgagga cgaaagcttt gacacggtga tcggtcacgc cgtgctccat cacattcccg
      661 acctcgacca agccttctcc gagttcttcc gggtgctgcg cccgggcggc cggatcgtct
      721 tctgcggcga gccttcccgc tacggagacc ggctggccgc agttcccaag cgaaccggaa
      781 acctcgtcgc cccggcctgg cgcaagctgg tcggcgccga caccctcgaa cacaccgagg
      841 aggagaaagc cgacgatgaa cacagtctcg agcccgaggt cgatgttcac gccttcgtcc
      901 cggccgacct gcgggcgatc ccgccgaggt ccggtttcgt cgagaccagg gtgagcggcg
      961 aggaactggt cgccaatgtc tggggctggg ggatgcgttc gatggaatcc accgccacgc
     1021 ccgattcgat cccgctgcgc tggcgaaact tcgccttcag gagctacatc gccttccaga
     1081 aggttgacaa caacctcctc gaaccccacc tcccggccga gctcttctac aacctcctgc
     1141 tttcggcgcg caaacccgcc tagccggtcg ctctaccgcc tcgcggggtc ggatcttgcc
     1201 ctcgcttcat gaggtgccgc acgtttagac ccttgatggt cctgtttttg cggcaccttt
     1261 gccgggcttt ggccgggtac ttgagctgcc gtttccggcc gttgacgaag tgcggcgtcg
     1321 tcctggtgtt tctggcgccg cccgggctac ttgcggcgta cgcgaaatag cttgacgggg
     1381 cggtcgagcc ccttgaactt gtgctggccg acccaggaat aggagaactc ctcctcgccg
     1441 gcggcgtcga ccgccttctt gtcggcgagc acgctgtcgg gccgggcggc ttcagtgatc
     1501 cggctggcca ggttcaccgg cgggccgaac cagtcaccga cctgcttgat cgcctcgccc
     1561 cgggcgacgc cgacccgcag ggcaggcagg tcctcgccgg cctcgttgcc gacctcgatg
     1621 aaccggatcg ccgcgtcaaa gagagcggcg ccgtcctcgg aaacgagcat cgcggcatca
     1681 ccgatcgtct tgaccaggcg gacctccggg ttggtcacct cgatcgccac cgactcgagc
     1741 agcttggcca cggttccgac cttctccagt tcggaacgct cgccgagccg ggtgaagccg
     1801 accaggtcgg cgaagcagat cgaaacctcg gtgctctcct tcggatcgaa gaagccgggg
     1861 ctggccatcg ccatcgcctg gtttctgagc tggtcgagga aatgtgaacg cagggcgtag
     1921 tcgaccgcct gctcggccag ggggagaagc tgcagcgagg cctggcgcag ctggactgcc
     1981 gcctcgtact cgtcggcgcc cggctcgatg atgtcggacg cgaccaggtc ccggtgcgac
     2041 tgggcgatcc gcgcggcaga gctgccgacc tgacgggtca ctgcgagcag gttctccttc
     2101 gggatgccgg cctcctgaaa gcgtttgagg cgccgcgccg cggtcaggtc ggcatcgtcg
     2161 aaggttcgct ggtccggctt gggtcgcggc aggccgaccg cggcaatcag gttctggaaa
     2221 tcgttgaccg acattccggc cagttcggca atttcggccg gggtgtactt gtcacgggca
     2281 cggcccagga ccagctccga gggaagcagg gccaggcggt tcgcgtccgc ttcctctttc
     2341 agggtgctca gctccacccc gtcctgcatg tagagctcgg tcagcagctt gatccgggcg
     2401 gcccggtcgt caccctccag tccttcgagc agcccctccg cttcgaaatc gggcaattcg
     2461 cgggtcgcca cgtcggccgc catgaagggc cgcaggtcgg gcgaagggta gtcgtccggg
     2521 tccgggtaat cagccatagg ccaccgcccc gccgcggcgc gggtcaccgg cgccgtcaag
     2581 ccggccggtt tcggggtcgc gggtcacgat ctgaacgccg ccgaaaaaca ggttgcgctg
     2641 attccagcgg aacacttcct gcccggccgc ctcgatccgg ccgagcgcct cttcatcgac
     2701 acccggctcg gcctgcagca cacccgcctc gaggtggatc cggggcgcat caaccgcagc
     2761 ctggggatca accccggcaa gcaggttcag ggtcgtctgc agcaccgccg aacggatccg
     2821 gttggacccg gcgctgccga gcccggcccg caccatgcct tgctcgagga tcagggtggg
     2881 tgacatcatc gaactgatcc tctcaccggg agcggactgg tggaagcccc tcgggttcag
     2941 atcttcctcg ccgagcatgt tgttgaggtg aaacccggtg ccgggcacga ccattccgga
     3001 cccggtcccg ttcgaacagg taaccgaggc gcagagacca tcggaatcga tcgccgccag
     3061 atgagtggtc gaaccgaggc ggtcgtccag aaacctgcgg gcgaagccct cctgaccaag
     3121 gctgagtgcg aagtcatcgt cccgggcttc gttggccgca gccatcgccc tggcgatttc
     3181 ctccgggcca cttccttcca tccgctcgag cagcgcgagc gagtaggcaa cgagaatccc
     3241 gccggccgaa ggcggcggat tggtcagcag ttcgcggccc cggtatcgcg ctccgaccgg
     3301 tgcccggacg atcggctcgt aggcctcgaa atcagccacc gagagggtgc ccccgcggga
     3361 gacgatgtat tcctcgacca gccggggaac ctcgcccgag cagagcgccc ccgccccctc
     3421 ggccccgaag cggtcgagcg cgtccccgag gtcgggatag cggaagacat ccccctggcc
     3481 gaggatcttt ccctcgggcg catagatcgc gcgggcttcc ggggtcgccg tgtagatcgg
     3541 ctcgaggatt ctcagcacat aggcctgttg ctcattgagc ggggcgcctt cccgggcaag
     3601 cgccgctgcg gggccggtca gctccgccat cggcaccgac ccgtaccgct ccagggcgag
     3661 agcgagaccg gccgccgtgc ccggaacccc gcaggaagca gccccggcat tgaagacctg
     3721 ggtggtcgac tcgtcgaagt gaaccggcac cggaagcaac tcctcggtca gttcgtgacc
     3781 gccccggccc ggcgcggcga cgaagaaatc gatcacggcg gtttcgattc cgggaccgcc
     3841 gacgatcatg aaaccaccgg cgccgagccc ggtcaacgca ctttcgagcg agaacgaggc
     3901 gagggcagcc gctaccgcgg cgtcgaccgc gttgccgcct tcacgcagga ttctggcgcc
     3961 ggcctcggcc gacagcgggt ggccggcggc aacgacaccg cgcccgtcca cagctcagga
     4021 gaggaattcg ggaatgtcgc tacccccgac tgccggcagc gattcgccac gaccgggccg
     4081 agcggtcgaa ccgccgcgat cccgccggtc ccgttcgctg cgctcgaccc gccgggcagg
     4141 agcacccttg ccgtcgaacc gggtcgccac gacggtgatc cagacctggt cgtcgagatc
     4201 gtcatcgatg ttcgcgccga agatgatgtt ggcgtcggga tgggctgcct caccgaccgc
     4261 acgggccgcc tcggaaacct cgatcagcga aaggtcgggg ccgccggtga ccgagagcag
     4321 gattgacttg gcgccatcca tcggggtttc cagcagaggt gagctggttg ccttctcggc
     4381 cgcgatcagc gcccggtcgg ggccgctgcc catgccgatt ccgagcagcg cccgaccggc
     4441 gtcggacatg atcgaccgca cgtcggcgaa gtcgaggttg atcaccgagg gcagcgtgac
     4501 caggtcggaa atgccctgaa caccctgccg cagcacgtcg tcggcaacct ggaaggcgtc
     4561 gaccatcggg gtcgactggt cgagcacctc gagcagccgg tcgttcggaa tcacgatcag
     4621 cgtgtcgact tcaccggcga gggcttcgat cccctggtcg gcctgcttct ggcgacggct
     4681 gccctcgaag gtaaacggct tggtcacgat cccgacggta agcgcaccga tgtcgcgggc
     4741 cagccgggca atgaccggcg cggcaccggt tccggtgcca ccaccggcgc cggccgcgat
     4801 gaagaccatg tcggagccct tgagcagccg cttgatccgg tcctgctcgt caaaggctgc
     4861 ccggaaaccg agcgaagggt ccgctccggc accgagtccg cgggtctcgt cgccaccgat
     4921 gtggacggtc aggtccgcca tgcagagctg gagcgactga aggtcggtgt tgaccgccat
     4981 gaactcgaca cccgggatct gggcctcgat catgcggttg atcgcgttga cgccaccccc
     5041 gccgactccg atcacccgga tcaccgggtt gtgtggaact ccggctccgg gagtcgagga
     5101 aggttcctga cggccgtagc ggggtccgtc gtcgcgatcc tgcggagcct cggagaaaat
     5161 ccgcttcagt cggtcctggg cgtcgggcac attggaagtt tcagcagccg ggtcgggggc
     5221 cgaaagcggg cccggatcct ccgcccgcgg ggcaggctcg gccggggtgg cgggagccgg
     5281 ttcttcctgt cggccctcca gcgggtcgcg gcgaccgaag cggccggcgg cgtcgggatc
     5341 gggctcgacg tgaacctcgg gctcaggtgc gggcgtcgag gctgcggctg cgggctcaac
     5401 cgggtcgccc tgcggtccgc cggtgtcgtc ggcggtacga ggttcgaatt cgggtggatc
     5461 ctgggagggt tcgtcggagg tagagcggaa caggtccgcc agcggacctt cacgcatact
     5521 cgctcgctta cgacgagcca tcactcaaga cctccttcgc cagattccgg tagtacgacg
     5581 ccgcacgact ctcgggatcg tacttcagga cactagcgcc tcgtaccggc gcctcggcga
     5641 acttcacggc cttgcggatt cgggacttga agacgaggtc cccgaaattc tcctcgagga
     5701 tgaacacggc ttcccgcgcg tgaagggtgc gggagtcgaa catggtcggc aggatgcctt
     5761 cgatgtggac gttcgggttc aggttctcct gaatcatctt cagggtgctc tgaagctgga
     5821 cgaggccgcg catcgagagg tactcgcact gaaccgggac gatcaccttg ctcgaagccg
     5881 tcagcgcgtt gatggtcagg agaccaaggc tcggtggggt atcgatgcag atgtagtcgt
     5941 agtccgagtg gacttccttg agcgccttct cgagcgaacg ttcccgcccg atcttggtgc
     6001 tcatggcgat ctcggcgccg gccaggtcga tcgaggcgac agcgatgtcg atctcccggt
     6061 gggcgatcac ttcggagatc ggcaggtcgt cgaccagcac gtcgtacatg ctcttttcgc
     6121 atttgtccgg gtcgattccc tggctcatgg tcaggttgcc ctgcgggtca aggtcgatgc
     6181 agaggacccg gttgccgagc tcggagaggg ccaccgccag gttcagggtg gaggtcgtct
     6241 tggcgacgcc acccttctgg ttggcgaagc tgatcacctc ggccatgtcg accggctgtt
     6301 ccggctgaac cggcttcagg tggtcgctca ggtcgcggcg gctcggggcg agcagatcgg
     6361 cggccttgag ccggatctcg gtctcttcgg cagccgcggc ttcctcgacc tcagccagtt
     6421 cctcctcgac cggcacggcg gctggctcgg cgggagtggc aggctcggca ggggcggcca
     6481 actcaggggg agtggccggc tcggcaggag tagcagccgc ttcgaccggc ccggggttcg
     6541 gctcaactgt ggccgtcgcc gcgggtgcga ccggttccgg aacagccgac tcgacttccg
     6601 gcacggtctc gacctcgggt ccctgctttt cacgttcctg gttcttgcgg ctcaagattc
     6661 gcatttgggc cgttattcgc atgtgaaagc cggtttcctc cccacaccct caatccggtt
     6721 gaatgccttc attcccgact ttctcgacaa acgcctgctc ttcgtcaccg gaaagggcgg
     6781 cgtgggcaaa accacggtcg ccatggctct cggtatccgc gccgcgatgg aaggcaagag
     6841 gaccatcgtc tgcgaggttg cggcgaccga aaacgcctcc cgtctttttg ctcagtccga
     6901 ggtcggcttc cgtgaagtcg aactggccaa cgacctctgg gcaatctcga ttgacccgga
     6961 cgagtcgatg cgcgagtacg tgctgctgca gctgaaagtc aaggcaatga gggacctgct
     7021 ttttcgcagc cgcatgttta cctaccttgc ggccgccacc cctggcctga acgagttggt
     7081 cacgatcggc aagatctggg agcttgccca gttcgaccgc aaggtcaaac acggccgcaa
     7141 atacgacctg gtcatcgttg acgcacccgc caccgggcac ggcatcagct tccttcagac
     7201 cccccgcacc ttcgcctcga tcgcccgggt cggcccgatc cacagccagg ccctgcagct
     7261 ggaggcgctg atcaccgacc atgaacagac cggcacggtg atcgtctcct tgccggagga
     7321 gatgccggta aacgaaaccg cccggctcga atcggagctt tccggtgaca tcggcatcgc
     7381 gatcgaccgt gtcgtgatga acgcgcttta cccggaaacc ttcccgaagg gcgatgtgaa
     7441 acggctgggc aagctgaaaa agagcgatga cgccgaggtc cgtgccgcgg gcagggccgg
     7501 cgcctcccag gccacccggg ccgcggccca gcgcgaacag ctcgcccggc tggaggaaat
     7561 ggtcgagacc gaggtcacca ccctcccctt catcttcaag ccggagctcg acctcaaggc
     7621 ggcccgccag ctggccgagg aaatcacctg atggcctctg tcgagcagat aatcgatggc
     7681 cgcgagatcg tgatctgcgc cggttcgggc ggtgtcggca agacgaccac atcggccgca
     7741 atcgcgcttg gaatggcggc tcgcggccac aaggtctgcg tgctcacgat cgatccggcc
     7801 aagcggctgg ccgattcact cggcctgaag gaactgggca acgaggccag caaggtgaac
     7861 ccgaagctgc tgaaagaaca gggcgtagat cccgaaaacg gcggtgaact ctgggcgatg
     7921 atgctcgacg caaaggagac cttcgacgcc ctggtcgctc gccacgccga agacgccgaa
     7981 gcccgcgacc gggtgctgaa taaccggatc taccagcaga tctccggagc cctggccggc
     8041 tcgcaggaat acatggcgat ggaaaagctc ttcgaacttc attcagaggg ccgtttcgat
     8101 ttgctggtcc tcgacccccc gccgagccgc aatgcccttg acttcctcga cgcgccacaa
     8161 cgcctgaccc agttcatcga aggcaagtcg ctgcagatct tcatccggcc gaccgggctt
     8221 gccgcccggg tcgccagccg aggcaccggg gtggcacttt cg
//
LOCUS       DAOQKN010000111         8222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_4220_length_8222_cov_5.057671, whole genome shotgun sequence.
ACCESSION   DAOQKN010000111 DAOQKN010000000
VERSION     DAOQKN010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 8222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8222
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4220_length_8222_cov_5.057671"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="PLJ64_08180"
     CDS             <1..868
                     /locus_tag="PLJ64_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93498.1"
                     /translation="AIWRRRDWFDANQVAFIALAGLSLYGLIQFSYFDNRSLATILPF
                     LCFPLLLSATIWLDLIQRDPGAGPALRRGAVAVALSLAAVTVSTVWPDARERSTDTAL
                     AYALPGGRSLADGLERLGDMPPLTGGADEGERLLRKYMPGESESAVATRYDLDLNILI
                     RADRANRLGIADANEASWVPGPHVPVMEEAVDSLASGDRMLVDQGALRQFSLLQQDPE
                     ALPDEFTTDDGQTVIQLMALARIGRRFDLKTVARGSDGLEVVELVPRGSGDELAGGKD
                     PRPVQDQAESGP"
     gene            complement(800..1564)
                     /locus_tag="PLJ64_08185"
     CDS             complement(800..1564)
                     /locus_tag="PLJ64_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNC93499.1"
                     /translation="MSEEATEETNRPKVIVVMPARQAAATLKATFDEIPRDAVDEVIL
                     VDDSSTDETVDLARQLEINVVWHPHQVGYGGNQKTCYLEALQRGADVVVMLHPDGQYE
                     PGLIPSMIEPIVADQADLVLGSRLLVEGWALSAGMPRWKFIANRFLTTIQNRIMGTRL
                     SEAHTGYRAYSRELLLTVPFLRNSLDFVFDSELLYQASYFGFRIREVPARCRYFDEMS
                     SVGFRTGVVYGLKTLWAGFRLILHRARILPSRKFIA"
     gene            complement(1557..2480)
                     /locus_tag="PLJ64_08190"
     CDS             complement(1557..2480)
                     /locus_tag="PLJ64_08190"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC93500.1"
                     /translation="MTRLKVDWTGAAATECVWCGRPLDARARRLTGRTICGSCGTGTT
                     DPLPDPESLDAAYGDWYWPSSGKRFGLVGDRMLRRSRASMAARIDEVAPPGPVLDVGA
                     GEGTLIDALKARGREARGLERLPSREDIVDLSLPEVEQDDFAAIIFWHSLEHLPDSGP
                     VMQAAAERLRPGGVAFVAVPDLSSPQARFFGDRWIHLDLPRHLVHLTTASLTAGMEAN
                     GLEVTATSRTRGGQNLIGWLDGFVAMLPGGLNLYQALRRKQARRIPLNPVQRLLSITA
                     GVLLAPVALVCAAVEVACRRSGTIYVEGRRV"
     gene            complement(2494..3261)
                     /locus_tag="PLJ64_08195"
     CDS             complement(2494..3261)
                     /locus_tag="PLJ64_08195"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC93501.1"
                     /translation="MGAKITGERVMTPEGGFNPTWQRHVAAYKLAEPFFGPGQLLDLG
                     CGVGHSFDLLAPRKTIGVDIDPGALEGQERETVVADMRRIPFEDGSFDSVLSVHSVEH
                     VPDPEKVVAEVSRVLRPEGVAVFVTPNRLTFGRPDEIIDPFHFIEFDAAQLEALCRAG
                     FREVETSGIFGTSRYMSIHDEERDRLDRLLGRDPLRLRRLVPVSAKQRLYDFMLRRSR
                     SLDDPRAEQITADDFRLESEGLDHCLDVVAVCRGPRQ"
     gene            3317..4903
                     /locus_tag="PLJ64_08200"
     CDS             3317..4903
                     /locus_tag="PLJ64_08200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNC93502.1"
                     /translation="MDGAEGQLGRMPWILVGVLTLTGLLFRVIVFGESGFGDELSTLW
                     IVRNNDLAGVVSIVHTDAEITPPLYFLLAKITSVFGPTISSVRLPSLLAGVGSLPLVY
                     AAGRRLIGSRGALAATLLATVSPFLVYFSAQARNYAVMIFLLLLATLLILEATAEGGR
                     WWHWAGWSLVAALALFSQYTAGFVILAQVLWVFWFFPAQRLRMAGFAVLAGLLFMPWI
                     TGLRADLDSPTADIMQALQGSSFTAKREAVEQLFFHWIEASPPTFFHRPDMMLATAGA
                     AVGLVAAAWRLAGGRLNLPQGERARGVWLAVAMVASVFICTLVLLALGTDIFGARNLA
                     PAWVGMPFLFGSVFVAAGRRAGWVFLILMIAGFMVSTIRLMDPNRSTINYSRVADQLD
                     DSGEKGTILDASIASPAPLTSLDAYLETDLPVFKMTAIEDRPDYIKQLFATYDAQKIL
                     DRAFSGAGPVRVVTADDFRAPREITSGGAAGGYEIQVNGQTAVIPPGWEPVSSHRYPG
                     LEPLTVTVFERSGQTGKSDR"
     gene            4900..6474
                     /locus_tag="PLJ64_08205"
     CDS             4900..6474
                     /locus_tag="PLJ64_08205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNC93503.1"
                     /translation="MNERIEKWLGAGRRFPVLIGLLAVAGFGLRMIQMGNPLFGDELS
                     TLWIVGNNGFFDSVSLVSSNAEITPPLYFMLSWLTTQLGSAPELVRIPALVAGTLTIP
                     MSALLGYRTVSRTTGLLAGLIVAISPYMTYFSANGRAYSLMVFFLLGSVLAMLAAIRT
                     GRTRWWVLFAVCAALSMYSHYMGGFVLAAALLWLLVLHPPQRKPALLATFCAAIAYLP
                     WIPSMLADNDSPTKEVMEHLQGDGFDQKRTQVEQWLVGQPLIEPGAVPGKLAMFLFAA
                     GLAVGLAGVVLKLVRDRDLMVGGKSVLPGLSLVALMTLSVPVAQLLLLLLGTDLFGAR
                     NLAPAWAGLPVLIGALISLPAFSLAAVATALTVGAFLIGAVRMTDPDLASTGYGGAAR
                     FIEANAGPGDVVMDSSHFTPVPLTELDTELSQDWPEYRVNLPIGDPPFLPVLAQVVPP
                     DEQIAEAFGKAAREDGKVFAVTWNPPLTDASKDGLYAPEGATRGIPKGWHLTETRTWD
                     GYRPVTVNVFEQSPSQ"
     gene            complement(6526..7302)
                     /locus_tag="PLJ64_08210"
     CDS             complement(6526..7302)
                     /locus_tag="PLJ64_08210"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNC93504.1"
                     /translation="MGEREAYTGRPRRYLPAGLLMWSGKSVSVVLMTYNEKDSIRHVI
                     EDFEATGVVDEVLVVNNNAARGTAEEVEATGARQVFETDQGYGHAIRRGLTEATGDLV
                     VLAEPDGTFLATDIIKLLAYSDECEVVLGTRTTNELIWEGANMDWFLKWGNWAVAKMV
                     ELIFNTSHLSDIGCTYRLLSRDVARKFAAEMQVGGSHAGPEMLMLTIRDGIRFVEIPV
                     NYLPRVGKSSVTGSRITAFFLGLTMIRLILGFRFGRRSVI"
     gene            7330..>8222
                     /locus_tag="PLJ64_08215"
     CDS             7330..>8222
                     /locus_tag="PLJ64_08215"
                     /inference="COORDINATES: protein motif:HMM:NF025089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC93505.1"
                     /translation="MIAIGSAITEEDIFDAYAGPGIERIREPDTELLIYPSAGSIFRS
                     YNVLLDKAKEIEGLEALVLIHQDAEIVDPDFFTKIRHGLSDPEVALVGCAGATGVRSI
                     AWWEGAVTWASYTHRYQEFGGGEIPAICWVYEDTPSFAGTGEVDSIDGFVIAFSPWAI
                     HNLRFDETIGGKLHGYDFDISMQVKEAGKKIVTEDLRVIHHHSLELISEMEGWVDTHM
                     QLSRKWQHLLSDNGGGEPDWEERARRAEAELSATRLMAGAGEIIWEARTQELERELNE
                     VKNSTSWKITRPLRRLRGSRE"
BASE COUNT         1302 a         2765 c         2752 g         1403 t
ORIGIN      
        1 tgcgatctgg cgccgccgcg actggtttga cgccaaccag gttgccttca tcgcgctggc
       61 cggcctcagc ctctacggcc tgatccagtt cagctacttc gacaatcgtt cgctggcgac
      121 gatcctcccc ttcctctgct ttccgctgct tctctcggcg acgatctggc tggatctgat
      181 ccagcgcgat cccggagccg ggccggcgct tcggcggggt gcggtcgcgg ttgccttgag
      241 cctcgcggcg gtcaccgtgt caaccgtctg gccggacgcc agggagcggt ccaccgatac
      301 cgctctcgcc tatgccctgc cgggaggccg cagcctcgcc gacggcctgg agcggctcgg
      361 tgacatgccg ccccttaccg gcggcgccga tgaaggagaa cggctgctcc ggaagtacat
      421 gcccggcgag agcgagagtg cggtggcgac ccgctacgac ctggacctga acatcctgat
      481 ccgggccgat cgggcaaacc ggctgggcat cgccgacgca aacgaggcga gctgggtgcc
      541 gggaccgcat gttccggtca tggaggaagc ggtcgactcg cttgcgtccg gagaccggat
      601 gctggtcgac cagggcgcac tccgccagtt cagcctgctt cagcaggacc cggaagcgtt
      661 gccggacgag ttcaccaccg atgacggcca gaccgtgatc cagctgatgg cattggcgcg
      721 gatcggccgg cgcttcgatt tgaagacggt tgccaggggc agcgacggac tcgaggtggt
      781 cgagctggtg ccccggggtt caggcgatga acttgcggga gggaaggatc cgcgcccggt
      841 gcaggatcag gcggaatccg gcccataggg tcttcagccc atagaccacc ccggtcctga
      901 aaccgaccga ggacatctcg tcgaaatagc ggcagcgggc cggaacctcg cggatgcgga
      961 acccgaagta ggaggcctga taaagcagct cggaatcgaa cacgaagtca agcgagttgc
     1021 gcaggaacgg aaccgtgagc agcagctcgc gggagtatgc gcggtaaccg gtgtgggcct
     1081 cggagaggcg ggtgcccatg atccggttct ggatcgtggt gaggaagcgg ttggcgatga
     1141 acttccagcg cggcatgccg gcggaaaggg cccagccctc gacgagcagg cgcgaaccga
     1201 gcacgaggtc ggcctggtcg gcgacgatcg gttcgatcat gctggggatg agccccggtt
     1261 cgtactggcc gtcggggtgg agcatcacga ccacgtcggc gccacgctgc agcgcctcga
     1321 ggtagcaggt cttctggttg cctccgtagc cgacctgatg gggatgccag accacgttga
     1381 tctcgagctg gcgggccagg tcgacggtct cgtcggtcga ggagtcatcg accaggatca
     1441 cctcgtcgac cgcgtcccgg ggaatctcgt cgaaggtggc cttcagggtg gcggcggcct
     1501 gccgggccgg catgaccacg atgaccttcg gacggttggt ttcctcggtt gcttcttcag
     1561 acacggcgac cctcgacgta gatggttccc gatcgccggc aggcgacctc gaccgcggcg
     1621 cagactaacg ccaccggggc caggagcacg ccggcggtga tcgaaagcag gcgctggacc
     1681 ggattgaggg ggatccgcct cgcctgtttg cggcgcagcg cctggtagag gttgagcccg
     1741 cccggcagca tcgcgacgaa gccgtccagc cagccgatca ggttctggcc gccgcgggtg
     1801 cgggaggtgg cagttacctc aagtccgttc gcctccatcc ccgcggtcag cgaggccgtg
     1861 gtcaggtgga ccaggtgcct gggcaggtcg aggtggatcc agcggtcgcc gaagaaccgg
     1921 gcctgggggc tggaaaggtc gggcaccgcc acgaaggcga ccccgccggg cctgagccgt
     1981 tcggcggcgg cctgcatcac cggccccgag tcgggcaggt gctccagtga atgccagaag
     2041 atgatcgccg cgaaatcgtc ctgctcgacc tccggcagcg agaggtcgac gatgtcctcg
     2101 cgggagggca gccgctcaag gcctcgggct tcccggcccc ttgccttgag ggcgtcgatc
     2161 agcgtgcctt cgccggcgcc cacgtcaagg accggcccgg gcggcgccac ctcatcgatc
     2221 cgggctgcca tcgaggcgcg cgaccggcgc agcatgcggt ccccgaccag gccaaagcgt
     2281 ttgcccgaac tgggccagta ccagtcgccg taggcggcat cgagcgattc cgggtccggc
     2341 aaggggtcgg tggtcccggt tccgcacgag ccgcagatcg tccggccggt cagccgccgg
     2401 gcccgggcgt ccagaggccg gccgcaccag acgcattcgg ttgccgcggc gccggtccag
     2461 tccaccttca gccgggtcac gcgcctgccc ccgtcactgg cggggacccc ggcagaccgc
     2521 gaccacgtcg aggcagtgat cgagaccttc cgattcgagc cggaagtcat cggcggtgat
     2581 ctgctcggcc cgggggtcat cgaggctgcg cgagcggcgc aacatgaagt catagaggcg
     2641 ctgcttggca ctgaccggaa ccaggcggcg caaacgcaag ggatcccggc cgagcagccg
     2701 gtcgaggcgg tcgcgttcct cgtcgtggat cgacatgtac cggctggtgc cgaagatgcc
     2761 gctggtttcg acctcgcgga acccggcccg gcagagtgcc tccagctggg ccgcatcgaa
     2821 ctcgatgaag tggaacgggt cgatgatctc gtccggccgg ccgaaggtga gccggttggg
     2881 agtcacgaaa accgcgacgc cttccggccg cagcacccgg gagacctcgg ccacgacctt
     2941 ctccggatcc ggcacgtgct cgaccgaatg gaccgaaagg accgagtcga aactgccgtc
     3001 ctcgaaggga atccggcgca tgtcggcgac cacggtctcg cgctcctggc cttccagggc
     3061 gccggggtcg atgtcgactc cgatcgtttt ccgcggggcc agcaggtcga agctgtgccc
     3121 gaccccgcag cccaggtcaa gcagctggcc ggggccgaag aagggttcgg ccagcttgta
     3181 ggcggcgacg tgccgctgcc aggtcggatt gaaccctccc tcgggcgtca tgaccctttc
     3241 cccggtgatc ttggcgccca ttggggcgaa tcttgacagg aaggacgatc aggcccgtag
     3301 taacctgcgc cctcctgtgg acggagcgga agggcagctc gggcgtatgc cctggatcct
     3361 cgtgggggtc ctcaccctca ccggtctcct gttccgcgtg atcgtgttcg gcgagtcggg
     3421 tttcggcgat gagctatcga ccctctggat cgtccgcaac aacgatctgg ccggggtcgt
     3481 ctcgatcgtt cacaccgacg ccgaaatcac cccgccgctc tacttcctgc tggccaagat
     3541 cacctcggtt ttcggtccga ccatctcctc ggtcaggttg ccgtcactcc tggcgggggt
     3601 cggaagcctg ccgctcgtct acgccgccgg gcgccgcctg atcggcagcc ggggtgccct
     3661 cgccgcaacc ctgctcgcca ccgtctcgcc cttcctcgtc tacttctcgg cccaggcccg
     3721 caactacgcg gtgatgatct tcctgctgct gctcgccacc ctgctgatcc tcgaggcgac
     3781 cgcggaaggc gggcgctggt ggcattgggc cggctggtcg ctggtcgccg ccctggcgct
     3841 cttcagccag tacacggcgg gcttcgtgat tctcgcccag gtcctctggg tgttctggtt
     3901 cttccccgcc cagcggctgc gaatggccgg ctttgcggtt ctggccggcc tgctgttcat
     3961 gccctggatc accgggcttc gggccgatct cgattcaccg accgccgaca tcatgcaggc
     4021 cctgcagggg tcgtcgttta ccgccaagcg ggaagcggtc gagcagctct tcttccactg
     4081 gatcgaggcc agccccccga ccttcttcca ccggccggac atgatgctcg ccacggccgg
     4141 ggcggcggtc gggctggtcg ctgcggcctg gcgcctggct ggtggccgac tgaatctgcc
     4201 gcagggcgaa agggcgcgcg gagtctggct ggcggtggcg atggtggcct cggtcttcat
     4261 ctgcactctg gtcctgctcg ccctcggaac cgacatcttc ggagcccgca acctggctcc
     4321 ggcctgggtc ggcatgccat tcctcttcgg ttcggtcttc gtcgccgccg gccgccgggc
     4381 cggctgggtc tttttgatcc tgatgatcgc cgggttcatg gtctcgacca ttcgcctgat
     4441 ggacccgaac cgctcgacga tcaattattc ccgggtcgcc gaccagctcg acgactcggg
     4501 tgagaagggg acgatcctcg acgcctcgat cgccagcccc gcgcccctga cctcgctcga
     4561 cgcctatctg gaaaccgacc tgccggtctt caagatgacc gcgatcgagg accgccccga
     4621 ttacatcaag cagctcttcg ccacctacga cgcccagaag atcctcgacc gtgccttcag
     4681 cggcgcgggt ccggtgcggg tggtgaccgc cgatgacttc cgggcgcccc gggagatcac
     4741 gagcggcggg gcggctggcg gatacgaaat ccaggtcaac ggacagacag ccgttatccc
     4801 gcccggctgg gaacccgtct ccagccaccg gtacccgggc ctggaaccgc tcaccgtcac
     4861 ggtcttcgag cgaagcggcc aaaccggaaa gagcgaccgg tgaacgagcg gatcgagaaa
     4921 tggctgggag cgggccggcg atttccggtc ctgatcggcc tgctggccgt ggccggtttc
     4981 ggcctgcgga tgatccagat gggcaacccc cttttcggcg acgagctttc caccctctgg
     5041 atcgtcggga acaacggctt ctttgactcg gtaagcctgg tttcgagcaa tgccgagatc
     5101 accccgccgc tctatttcat gttgagctgg ctgaccaccc agctcggttc ggcgccggaa
     5161 ctggtccgga tcccggccct ggttgcgggc accctgacga ttccgatgtc ggccctgctc
     5221 ggttaccgca cggtcagccg caccaccggc ctgctcgccg gtttgatcgt cgcgatcagc
     5281 ccgtacatga cctacttctc ggccaacggc cgggcctact cgctgatggt cttcttcctg
     5341 ctcggctcgg tcctggcgat gctggcggcg atccggaccg gccgcacgcg ctggtgggtg
     5401 ctcttcgcgg tctgcgcggc gctctcgatg tactcgcatt acatgggcgg tttcgtgctc
     5461 gccgcggcgc tcctgtggct gctggtcctt cacccgccgc aaaggaagcc ggccctgctg
     5521 gccacgttct gcgccgcgat cgcctacctc ccctggatcc cgagcatgct ggccgacaac
     5581 gattccccga ccaaggaggt gatggagcat ctccagggtg acggcttcga ccagaagcgc
     5641 acccaggtcg agcagtggct ggtcggccag ccgctgatcg agccgggagc cgtacccggc
     5701 aagctggcga tgttcctctt cgcggccggg cttgcggtcg ggctggccgg cgtcgtcctg
     5761 aaactggttc gtgaccggga cctgatggtc ggggggaagt cggtcctgcc cgggctcagc
     5821 ctggttgccc tgatgaccct ttcggttccg gtcgcgcagc tgctcctgct tctgctcggc
     5881 accgatcttt tcggcgcccg caacctcgcc ccggcctggg ccgggctgcc ggtcttgatc
     5941 ggggccctga tcagcctgcc cgccttctcg ctggccgcgg tagcgacggc attgacggtc
     6001 ggcgccttcc tgatcggggc ggtgcggatg accgacccgg acctggccag caccggttac
     6061 ggcggcgcgg cccgtttcat cgaggccaac gccgggccgg gggatgtggt gatggactcg
     6121 agccatttca ccccggtgcc gctgaccgag ctcgacaccg agctgtcaca ggactggccc
     6181 gagtaccgcg tgaacctgcc gatcggcgac ccgccgttcc tgccggtcct ggcccaggta
     6241 gtgccgccgg acgagcagat cgccgaggcc ttcggcaagg cagcccggga ggatggaaag
     6301 gtcttcgccg tcacctggaa cccgcccctg accgatgcca gcaaggacgg gctttacgcg
     6361 cccgaaggtg cgacccgggg cattccgaag ggctggcacc tgaccgaaac caggacctgg
     6421 gatggctatc gcccggtcac ggtgaacgtc ttcgagcagt cgccttcgca ataaggagaa
     6481 ggagccggac tggcgcgcaa ccgcagggcc gaacccggcg gccggtcaga tcacggaacg
     6541 gcgaccgaag cggaagccca ggatcaatcg gatcatggtc aggccgagga agaaggcggt
     6601 gatgcggctg ccggtcaccg aggacttgcc gacccgcggc aggtagttga ccgggatctc
     6661 gacgaagcgg atcccgtcgc ggatggtcag catcagcatc tcggggccgg catgggagcc
     6721 gccgacctgc atctcggccg cgaacttgcg ggccacatcc cggctcagca gccggtaggt
     6781 gcagccgatg tcactgaggt ggctggtgtt gaagatcagc tcgaccatct tcgccacggc
     6841 ccagttgccc cacttgagga accagtccat gttggcccct tcccagatca gttcgttggt
     6901 ggtgcgggtc ccgagcacga cctcgcattc atccgaatag gcgagcagct tgatgatgtc
     6961 cgtggcgagg aaggtgccgt ccggttcggc caggacgacc aggtcaccgg tcgcctcggt
     7021 gagcccgcga cggatcgcgt ggccgtaccc ctgatcggtc tcgaagacct gccgggcacc
     7081 ggtcgcctcg acttcctcgg ccgtgcccct ggcggcgttg ttgttcacca ccagcacctc
     7141 gtcgaccacc ccggtcgcct cgaagtcctc gatcacatgc cggatcgagt ccttctcgtt
     7201 gtaggtcata aggaccacgc tgactgattt cccgctccac ataagaagcc cggccggaag
     7261 gtaccggcgg gggcggccag tataggcttc gcgctcgccc atgactgagc ccacgcaggg
     7321 gagaaaccaa tgatcgccat cggcagcgcg atcaccgaag aggacatctt cgacgcctat
     7381 gccggtcccg gcatcgagcg cattcgcgag ccggacaccg aactgctgat ctacccgtcg
     7441 gccggatcga tcttccgcag ctacaacgtg ttgcttgaca aggcgaagga aatcgagggt
     7501 ctcgaggcgc tggtcctgat ccaccaggac gccgaaatcg tcgatcccga cttctttacg
     7561 aagatccgcc acgggctgag cgaccccgag gtcgctctgg tcggctgcgc cggtgcgacc
     7621 ggggtccggt cgatcgcctg gtgggagggt gcggtcacct gggcctccta cacccaccgc
     7681 taccaggagt tcggcggcgg cgagatcccg gcgatctgct gggtgtacga agacacgcct
     7741 tccttcgccg gcaccggtga ggtcgattca atcgacggtt tcgtgatcgc cttctcaccc
     7801 tgggccattc acaacctccg cttcgacgag acgatcggcg gcaagcttca cggttacgac
     7861 ttcgacatct cgatgcaggt aaaggaagcc ggcaagaaga tcgtcaccga ggacctgcgg
     7921 gtcattcacc accactcact cgagttgatc tccgagatgg agggctgggt ggacacccac
     7981 atgcagcttt cgcgcaagtg gcagcacctg ctctccgaca atggtggcgg cgagcccgac
     8041 tgggaggagc gagcccgccg ggccgaggcc gagctttccg cgacccgcct gatggccggc
     8101 gccggagaga tcatctggga ggcccgtacc caggaactcg aacgggaact gaacgaggtc
     8161 aagaacagca cctcgtggaa gatcacccgc cccctccgcc gcctccgcgg cagccgcgaa
     8221 aa
//
LOCUS       DAOQKN010000112         8219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_4222_length_8219_cov_7.438633, whole genome shotgun sequence.
ACCESSION   DAOQKN010000112 DAOQKN010000000
VERSION     DAOQKN010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 8219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8219
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4222_length_8219_cov_7.438633"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2130)
                     /locus_tag="PLJ64_08220"
     CDS             complement(<1..2130)
                     /locus_tag="PLJ64_08220"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HNC93506.1"
                     /translation="MNFAACRPSRASLPVFACLLIAAFVLPAFLPAPAEARFGQWLNF
                     GSSYGVPSGRIGIQFTRDVGLDESKATGNRPGGRVGFLSGGVWYHATRLADTVALPDP
                     SILNTNDPAGRQIRIEVRDIGNETMRLSAEVLGSGPEPVDSIGIGFGLKDSERLLGFG
                     ERSDHVNQRGNEVESYVGEGPYQETDYPIIKNTVPSWGVRERSDATYFPMPWFISTRG
                     YGVLAGNAETSRFRLGTESKGEWSFEADARRMTLRFFAGPRPAGVLRRMTRAIGRQPK
                     PRAPWIFGPWFQTGHQNTSPNELDYVETLRDADAPVSAVETHMRYMPCGSDLGQEAAE
                     KARVAGLHAGGLAAITYTREAICASYSGPYDQAFSQGAFIRHQDGTPYTFNSFVGSGV
                     TQLGMLDFTNPAAQPIYAGILDRAYTAGYDGWMEDYGEYAPPDSVSFDGIPGKRLHNL
                     HPVFYHRGGLRYSNSKKRPIVSFVRSGFTGSARYSQIVWGGDPTTGWGFDGLQSQVTE
                     ALTMGLSGVSNWGSDIGGFFSFSPQKLDAELLARWIEFGAFSGVMRSKAEGIGASMDS
                     RPQIWEPETLPVWRRYAKLRTQLYPYLAAANATYRRTGMPVMRHLALTNPTDRRATGI
                     DDQYMFGPSLLVSPVLAPGTGARAVYLPKGKWVDFWQAVSYDEADGSFNLGRAKLLGG
                     GHEVKASAPLEEIPVMAKAGTLLPLLPA"
     gene            2172..2666
                     /locus_tag="PLJ64_08225"
     CDS             2172..2666
                     /locus_tag="PLJ64_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93507.1"
                     /translation="MSSLKGVSAVLALLVLGLFAYGCGGDDGATQEQLDAAKAEGAKE
                     ARQQARLNQLQGQVKQLQKQSKQSSNGNSGKTGGQGDGYYQDAGSDGGPVSTCATGVQ
                     VGPNTSCSFAMNVAGEYGSNPGASTISAYSPVTGEYYTMSCGSWSGGGTVCTGGNGAA
                     VYMP"
     gene            2663..3637
                     /locus_tag="PLJ64_08230"
     CDS             2663..3637
                     /locus_tag="PLJ64_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93508.1"
                     /translation="MTGLRRFALAGLCLGLLLAGCGGSDDQATIKPAPDRSARISQLE
                     QRKQNLELQLKAQRKKEAAEAEAKTAEVGGDADANALLTGLPGEAGLVVGAPGGGGPD
                     FSGGSLSSGDAWSTIKVPIAERVLDDFGGPDGISPNQAANIASAITLSDNDAAASLFT
                     DLEGKHGGLEGASAAVGEMLRQAGDRETIISTVGRDGFSTYGQTDWSLENQFRYMAAL
                     AGGCIGNAGNRAYLLDQMARVGGSDTFGLGAAGFPARWKGGWGPGTDGKYLVRQMGVM
                     VVNGKQVVVSMAAIPDDGTFESGASMLSAIARSVASRLAGRVSAPTGC"
     gene            3687..4136
                     /locus_tag="PLJ64_08235"
     CDS             3687..4136
                     /locus_tag="PLJ64_08235"
                     /inference="COORDINATES: protein motif:HMM:NF017936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNC93509.1"
                     /translation="MDRETVIRELRLEPLPKEGGMYRRKFKDDNSSSIYFLIEPDSPT
                     NLHRLPWPELFHFYAGAPARLHLLGPEPGEARHPVLGIGLEEGERPQMLVPAGTWQAA
                     ETTGAWTLLGTTMAPPFDWDRFELGKRERLLKYWPDQREVIERHLAT"
     gene            complement(4146..5645)
                     /locus_tag="PLJ64_08240"
     CDS             complement(4146..5645)
                     /locus_tag="PLJ64_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNC93510.1"
                     /translation="MNPNLATLLTESAEKFPERVAIRLDETEINYAALDQVSKLVAGL
                     LQSKGVEAGDRVGIMLPNVPHFPMVYYGILRMGARVVPMNVLLTAREIKHYLGNSGAK
                     LVVAWEDFAPAAIEAAEEVGAEVISINPTNVADLIQGIEPYEGVAEVEPDDTAVILYT
                     SGTTGQPKGAELTHSNIRSNIESIGDLFTPTEEDVFFGGLPLFHVFGQTVAMNASIKT
                     GAELTLLPRFDPAKALEIIQRDKVTIFQGVPTMYAAMLQVPNRADYDISSLRLCVSGG
                     AALPVEIIRAFEAEFNTPILEGYGLSETSPVACFGRVDMERKAGTIGTPIRDVELRLV
                     DEEGNVLGVGDVGELQIKGPNVMKGYWQMPEATEKAIDADGWFASGDMATVDEDGYYS
                     IVDRKKEMILRGGYNVYPREVEEVLYEHPAVAEAAVMGVPHDELGEEIVAIIAFKEGE
                     SATEDELRDHAKEGVAAYKYPRHILIVDELPKGPTGKILKREIDLSQVG"
     gene            5728..6477
                     /locus_tag="PLJ64_08245"
     CDS             5728..6477
                     /locus_tag="PLJ64_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC93511.1"
                     /translation="MSFSEKVVLITGGANGFGRATGARLAGEGAHVVLADIEAEAGQE
                     AASEIGAEFIHCDVRDPEASVAAVDFAVEKFGGLDVAFLNAGISTGCGLADTFDLELY
                     RRAMQINLDGVVFGINAAVPAMRRRGGGSIVATASLAGLTAVPFDPIYAANKHGVVGL
                     VRSAGPVYALESIRINGICPGFADTRIIDGFKENLENEGVPIITVEAVVDGIVDLMAS
                     DESGVCHFVQAGMDPQEFRFRNIPGPRAAEA"
     gene            complement(6503..6955)
                     /locus_tag="PLJ64_08250"
     CDS             complement(6503..6955)
                     /locus_tag="PLJ64_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93512.1"
                     /translation="MKTVSTKPGAVQIDLLRNAIAFVSTNWDEDLAAALDGREGMSKL
                     RALASWSLQTNNYDDAIRRLWIEALAGTAPEATEEIAAAKEQAQSEYQFVLAVTKEAG
                     QDKQLADALFLAFRGYYLSSIEDPEHWPAERAQEAVLKVLDMLDKRAR"
     gene            complement(6952..8168)
                     /locus_tag="PLJ64_08255"
     CDS             complement(join(6952..7880,7880..8168))
                     /locus_tag="PLJ64_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612098.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HNC93513.1"
                     /translation="MSRSKRYPREFRERSVALVLEHQGEHRSQWACIQSVAAKSGMTP
                     ETLRRWVRQAEVDSGGRAGVSTDERKRIRELERENRELRRANEILKAASAFFGAGARP
                     ASTEEMTAFIEEHREKFGIEPICQIMDFAPSTYYAALKRRENPSARDLRDQELIPEIR
                     RVHRENLGVYGARKTWLQLKREGIMVPRCQVERLMREEGLQGVVRGRKAPRTTFPGGQ
                     AKRALDLVDRKFEAQAPNRLWVADFTYVFTWEKLCFAAFVIDVFSRRIVGWRVADSMR
                     TELVLDAFEMALWSRGHEDLPVGEGLVVHSDAGSQYTSFAFTRRLIDAGVDASIGSVG
                     DAYDNALAESTIGLYKTELINKDGPWRTRSDVELATLTWVDWFNNRRLHGACGDLPPI
                     EFEQQHQAASITQ"
     misc_feature    complement(7774..7890)
                     /locus_tag="PLJ64_08255"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
BASE COUNT         1395 a         2625 c         2729 g         1470 t
ORIGIN      
        1 ggccggcagc agcggcagca gcgttcccgc cttcgccatc acggggatct cctccagcgg
       61 ggcgctggcc ttgacctcgt ggccgccgcc gagcagcttc gcccggccga ggttgaagct
      121 gccgtcggcc tcgtcgtagg agaccgcctg ccagaagtcg acccacttgc ccttcggcag
      181 gtagaccgca cgggcgccgg tgccgggggc gaggaccggc gagacgagca ggctgggccc
      241 gaacatgtac tggtcgtcga tcccggtcgc ccgtcggtcg gtcgggttgg tcagcgcaag
      301 gtggcgcatg accggcatcc cggtccggcg gtaggtcgcg ttggcggccg ccaggtacgg
      361 gtagagctga gtgcgaagct tggcgtagcg gcgccagacg ggcagcgttt ccggctccca
      421 gatctgcggc cgggagtcca tcgaagcgcc gatgccttcc gccttcgagc gcatcactcc
      481 ggagaaggcc ccgaactcga tccagcgggc gagcagttcg gcgtcgagtt tctgcggcga
      541 gaagctgaag aagccgccga tgtcggagcc ccagttggag acgcccgaaa ggcccattgt
      601 cagggcttcc gtgacctgcg actggagccc gtcgaagccc cagcctgtgg tcgggtcgcc
      661 gccccagacg atctgcgagt agcgggccga gccggtgaag ccggaacgca cgaagctcac
      721 gatcggccgc ttcttcgagt tcgagtagcg caggccgccc cggtggtaga agaccgggtg
      781 gaggttgtgc aggcgcttgc cggggatccc gtcgaaggaa accgagtccg gcggggcgta
      841 ctcgccgtaa tcctccatcc agccgtcgta gccggcggtg taggcgcgat cgaggatgcc
      901 ggcgtaaatc ggctgggcgg ccggattggt gaagtcgagc atgccgagct gggtcacccc
      961 gctgccgacg aaggaattga aggtgtaggg ggtgccgtcc tggtggcgga tgaaggcgcc
     1021 ctgcgagaag gcctgatcgt acggcccgga gtaggaggcg cagatcgcct cgcgggtata
     1081 ggtgatcgcg gccaggccac cggcatgcag tccggcgaca cgggccttct cggccgcttc
     1141 ctgaccgagg tcggaaccgc agggcatgta gcgcatgtgt gtctcgaccg cggagaccgg
     1201 ggcatcggcg tcgcgcaggg tctcgacgta atcgagttcg ttgggtgaag tgttctggtg
     1261 gccggtctgg aaccaggggc cgaagatcca gggtgccctc ggcttaggct ggcggccgat
     1321 cgccctggtc atgcgccgca gcaccccggc cggtcggggt ccggcaaaga agcgcagggt
     1381 catccggcgg gcatcggcct cgaaggacca ttcgcccttg ctctcggttc cgagccggaa
     1441 gcggctggtt tcggcattgc cggcgagcac gccgtaaccg cgggtggaga tgaaccaggg
     1501 catcgggaag taggtcgcgt cggagcgctc acgcactccc caggacggga ccgtgttctt
     1561 gatgatcggg taatcggttt cctggtaggg gccctcgccc acgtagcttt cgacctcgtt
     1621 gccgcgctgg ttcacgtggt cggagcgctc gccgaacccg agcagccgct cgctgtcctt
     1681 caggccgaac ccgatcccga tcgagtcgac cggttccggg ccgctgccga gaacttcggc
     1741 gctcagccgc atcgtctcgt tgccgatgtc gcgcacttcg atcctgatct ggcggccggc
     1801 cgggtcgttg gtattcagga tcgacgggtc gggcagggcg acggtatcgg ccagccgcgt
     1861 ggcgtggtac cagacaccgc ccgaaagaaa gccaacccgg ccgcccgggc gattgccggt
     1921 cgccttcgat tcatcgaggc cgacgtcacg ggtgaactgg atcccgatcc tgccgctcgg
     1981 caccccgtag gaggagccga aattgagcca ctggccgaac ctggcttcgg ccggcgcggg
     2041 caggaaggcg ggcaggacga aagcggcgat cagcaggcag gcgaatacgg gcagggaggc
     2101 gcgtgacgga cggcaggcag caaaattcat gagttcttcc agaacactac gaccagggct
     2161 aagcttcggt catgtccagt ctcaagggag tatccgccgt cctcgccttg ctcgttctcg
     2221 gcctctttgc atacggatgc ggtggtgacg acggggcaac ccaggagcag ctcgatgcgg
     2281 cgaaggccga gggcgccaag gaagcccgcc agcaggccag gctgaaccag cttcagggcc
     2341 aggtcaagca gctgcagaaa cagtcgaagc agagctcgaa cggcaactcc ggcaagacag
     2401 gcggccaggg cgacggctac taccaggacg ccggttcgga cggtggcccg gtttcgacct
     2461 gcgcgaccgg ggtgcaggtc ggcccgaaca cttcctgttc cttcgcgatg aacgtggccg
     2521 gtgagtacgg cagcaacccc ggtgcttcta cgatcagcgc ctacagcccg gtgaccggcg
     2581 agtactacac gatgagctgc ggctcctgga gcggcggtgg caccgtctgc accggtggaa
     2641 acggcgctgc cgtctacatg ccttgaccgg cctgcggcgg ttcgcgctgg ccgggctctg
     2701 ccttggtctg ctcctggccg gttgcggcgg atcggatgac caggcgacga tcaagcccgc
     2761 ccctgaccgc agcgcccgga tttcgcagct tgagcagcgc aaacagaacc tggagcttca
     2821 gctgaaggcg cagcgaaaga aggaggcggc ggaagccgag gcgaagaccg ccgaggtggg
     2881 cggggacgct gacgcgaacg ccctgctgac cggcctgccc ggcgaggcgg gcttggtcgt
     2941 cggcgctccc gggggtggcg ggcccgactt cagtggaggc tcgctttcca gcggcgatgc
     3001 ctggtcgacg atcaaggtgc cgatcgccga gagggtcctc gatgatttcg gcgggccgga
     3061 cgggatcagc ccgaatcagg cggccaacat cgcgagcgcg atcaccctct ccgacaatga
     3121 cgcggccgcc tcgctcttta ccgacctgga aggaaaacac ggcggcctcg aaggggcctc
     3181 ggcggcagtc ggggaaatgc tccggcaggc cggtgaccgg gaaaccatca tttcgacggt
     3241 gggccgggac ggtttctcga cctatggcca gaccgactgg tcgctggaaa accagttccg
     3301 gtacatggcg gcactggccg gaggctgcat cggcaacgcg ggcaaccgcg cctacctgct
     3361 ggaccagatg gcccgggtcg gcggcagcga caccttcggg ctcggcgcag ccggcttccc
     3421 cgcgaggtgg aagggaggct ggggcccggg taccgacggc aaatacctgg ttcgccagat
     3481 gggcgtgatg gtcgtgaacg gcaaacaggt cgtggtcagc atggccgcga ttccggatga
     3541 tggaaccttc gagtccggcg cgtcgatgct gagcgcgatc gcgcgatccg tcgcctcgcg
     3601 cctggccggc cgcgtttccg ccccgactgg ctgctgactg tctgccagcc ggggcaaacc
     3661 ggagcaaact ggcgtatcgt ccgggtatgg atcgcgaaac ggtaatccgc gagctgaggc
     3721 tcgagccgct gcccaaagag ggcggcatgt accggcgcaa gttcaaggac gacaactcca
     3781 gctcgatcta ctttctgatc gaaccggatt caccgaccaa ccttcaccgg ctcccttggc
     3841 ctgagctctt tcacttttac gccggcgccc cggcccggct gcatctgctc ggtcccgagc
     3901 ccggagaagc gagacacccg gtgctcggca tcgggctcga agagggcgag cgtccgcaga
     3961 tgctcgtgcc cgccggaacc tggcaggcgg ccgagacgac cggcgcctgg accctgctcg
     4021 gcacgaccat ggcgccgccc ttcgactggg accgcttcga actaggcaag cgcgagcgcc
     4081 tgctcaagta ctggcccgat cagcgcgaag tgatcgaacg ccacctcgcc acctgagcac
     4141 caggctcagc caacctggct gagatcgatt tcgcgcttca ggatcttgcc ggtggggccc
     4201 ttggggagct cgtcgacgat caggatgtgg cgcgggtact tgtaggcggc gacgccttcc
     4261 ttcgcgtggt cgcgcagctc gtcttcggtc gccgactcgc cctccttgaa ggcgatgatc
     4321 gcgacgatct cctcaccgag ttcatcgtgg gggacgccca tcacggcggc ctcggccacg
     4381 gccgggtgct cgtacagcac ctcttcgacc tcgcggggat agacgttgta accgccgcgc
     4441 aggatcatct ccttcttgcg gtcgacgatc gagtagtaac cgtcctcgtc gacggtcgcc
     4501 atgtcgcccg aagcgaacca gccgtcggca tcgatcgctt tctcggtcgc ttccggcatc
     4561 tgccagtagc ccttcatcac gttcggaccc ttgatctgaa gctcaccgac atcgccgact
     4621 ccgagcacat tgccctcctc gtcgaccagc cgcagttcca catccctgat cggggtgccg
     4681 atcgtgccgg cctttcgctc catgtcgacc cggccgaagc aggcgaccgg agaagtctcg
     4741 gagaggccgt aaccctcgag gatcggcgtg ttgaattcgg cctcgaaggc gcggatgatc
     4801 tcgaccggaa gggcggcacc gccggaaacg cagaggcgga gcgagctgat gtcgtaatcg
     4861 gcccggttcg gcacctggag catcgccgcg tacatggtcg gcaccccctg aaagatcgtc
     4921 accttgtcac gctggatgat ctccagagcc ttggccgggt cgaacctcgg cagcagggtg
     4981 agctcggccc cggtcttgat cgaggcattc atggcgacgg tctgaccgaa gacatggaag
     5041 agcggcaggc cgccgaagaa gacgtcctcc tcggtcgggg tgaagaggtc gccgatcgac
     5101 tcgatgttcg agcggatgtt cgagtgcgtg agctcggcgc ccttgggctg gccggtggtg
     5161 ccggaggtgt agagaatgac cgcggtgtca tcgggctcga cctcggccac gccttcatag
     5221 ggttcgatcc cctggatcag gtcggccacg ttggtcgggt tgatcgagat cacctcggcc
     5281 ccgacttcct cggcggcttc gatcgcggcc ggtgcgaagt cttcccaggc gaccacgagt
     5341 ttcgctccgg agttgccgag gtaatgcttg atttcacggg cggtcagcag cacgttcatc
     5401 ggaacgacgc gcgcgcccat ccgcaggatc ccgtaataga ccatcgggaa atggggaacg
     5461 ttggggagca tgatcccgac ccggtcgccg gcttcgacgc ccttggactg gagcaggccg
     5521 gcaaccagct tgctcacctg atcaagcgct gcatagttga tctcggtttc gtcgaggcgg
     5581 atcgcgacgc gctcggggaa cttctctgcg gactcggtca gcagcgtggc cagattcgga
     5641 ttcaactcgg tacctctccc gggtagttca acagcgggac aaaaccgcaa tttccggcct
     5701 gccctcttag atggcatgct acccggcatg agcttttcgg agaaggtggt actgatcact
     5761 ggcggcgcca acgggttcgg ccgggcgacg ggggcgcgac tcgcgggcga aggggcccat
     5821 gtggtgctcg ccgacatcga ggctgaagcg ggtcaggagg cggcctccga gatcggggcc
     5881 gagttcatcc attgcgatgt ccgggacccg gaagctagcg tcgccgcggt cgacttcgcg
     5941 gtcgagaagt tcggcgggct ggacgtggcc tttctgaacg ccggcatctc gaccggttgc
     6001 gggctggccg acaccttcga tctcgagctt taccgccggg caatgcagat caacctcgac
     6061 ggagtggtct tcgggatcaa cgccgcggtg ccggcgatgc gccggcgggg tggcggttcg
     6121 atcgtcgcca ccgcctcgct ggccggactg accgcagtgc ccttcgaccc gatctacgcg
     6181 gccaacaagc acggcgtggt cggccttgtc cgctccgccg gtccggttta cgccctggag
     6241 agcatccgca tcaacggcat ctgccccggc tttgccgaca cccggatcat cgatggcttc
     6301 aaggagaacc tcgaaaacga aggggtgccg atcatcacgg ttgaggccgt ggtcgacggg
     6361 atcgtcgatc tgatggcctc cgatgaaagc ggcgtctgcc acttcgtcca ggccggcatg
     6421 gacccccagg agttccgctt ccgcaacatc cccggcccga gggccgccga ggcctgagcc
     6481 ggcctccgct tcagccgcgt gactaccggg cgcgtttgtc cagcatgtcc aggactttga
     6541 gcaccgcctc ctgcgctctc tcggcaggcc agtgttccgg gtcttctatc gatgagaggt
     6601 aataccccct gaacgcaagg aaaagcgcat cggccaattg cttgtcttgt cccgcttctt
     6661 ttgtcaccgc taggacgaat tggtactcgc tttgcgcctg ttccttcgca gcggcgattt
     6721 cctcggttgc ttccggggcg gtgccggcga gagcctctat ccatagccgg cggatcgcgt
     6781 cgtcgtagtt gttggtctga agcgaccagc ttgcgagagc tctgagcttg gacatgcctt
     6841 cgcgtccatc gagagccgcg gcaagatctt catcccagtt ggttgacacg aacgcaatcg
     6901 cgtttctcag caggtcgatt tgaaccgccc cgggttttgt ggagacggtt ttcattgggt
     6961 gattgatgct gcttggtgtt gctgttcgaa ctctatgggt ggcaggtctc cgcaggctcc
     7021 atggagcctg cggttgttga accagtcaac ccaggtcagg gttgccagtt caacgtctga
     7081 gcgggtccgc cagggtccgt ccttgttgat cagctcggtt ttgtagagac cgatggtgga
     7141 ttccgccagc gcgttgtcgt aggcgtcccc gaccgatccg attgaagcgt cgacgccggc
     7201 atcgatgagc cggcgagtga acgcaaacga tgtgtattgg ctaccggcgt cggagtgaac
     7261 cacgagtccc tcgccgaccg gaaggtcttc gtggcctctg gaccagagcg ccatctcgaa
     7321 cgcatcgaga accagttcgg ttcgcattga gtcagcgacc ctccagccaa cgatccggcg
     7381 ggaaaacacg tcgatcacga acgccgcaaa gcaaagcttc tcccacgtga acacgtaggt
     7441 gaaatcagcg acccagagcc ggttcggtgc ctgggcttcg aacttccggt caaccagatc
     7501 cagggcccgt ttagcctgcc ctccaggaaa ggttgttctc ggggctttcc ggcccctgac
     7561 cacgccttga agaccttcct cgcgcatcag gcgttccacc tggcagcgcg gcaccatgat
     7621 gccctcccgt ttcagctgaa gccaggtttt ccttgccccg tagacaccaa ggttttcccg
     7681 gtggaccctc cggatctccg ggatcaactc ctggtcacga agatctctcg ctgacgggtt
     7741 ctctcttcgt ttgagtgccg catagtaggt ggacggggcg aaatccatga tctggcagat
     7801 cggctcgatc ccgaacttct cgcgatgctc ctcgatgaac gcggtcatct cttcggtgga
     7861 tgcgggtcga gctcccgcgc gaaaaaagcc gaagcggcct tcaggatctc gtttgctctc
     7921 ctgagctccc ggttttccct ttcgagttca cgaatgcgtt ttcgttcatc ggtgctgacc
     7981 ccggcccggc caccggaatc aacctccgcc tgcctgaccc aacgcctcaa cgtctccggg
     8041 gtcatccccg acttcgccgc gaccgactga atacacgccc actgcgagcg atgctcgccc
     8101 tggtgctcga gcaccagggc aacagaacgc tcccggaact cccgcggata cctcttcgac
     8161 ctagacattt gaccgatcct ctcaaagcaa ttggcctcca acaaacccgg ggcggttca
//
LOCUS       DAOQKN010000113         8093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_4327_length_8093_cov_5.522020, whole genome shotgun sequence.
ACCESSION   DAOQKN010000113 DAOQKN010000000
VERSION     DAOQKN010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 8093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8093
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4327_length_8093_cov_5.522020"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1416
                     /locus_tag="PLJ64_08260"
     CDS             <1..1416
                     /locus_tag="PLJ64_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNC93514.1"
                     /translation="PVKRVIEGEPEEGGDDRGLPYAGDGPMTGSGRFDGMNNRAAYDE
                     IVEWLKLEGRGDASVNFRLRDWLVSRQRYWGAPIPIVYCDECGMVPVPEEQLPVRLPE
                     IDDYAPKGKSPLAAATHWVATTCPKCGAAAERETDTMDTFVDSSWYFLRYLDAQNSEA
                     AWDREAADFWMPVDQYIGGVEHAILHLMYARFFAKALKDMGHIGVVEPFANLFTQGMI
                     TRDGAKMSKSKGNTVSPSEIVARHGADAARTYVCFMGPPERSGDWTDEGVEGVHRFLA
                     RLWRLGEELAAAGQSGPLALPDGLDGEAREVAAKAHWAIDKATGDFNRGFQFNTVIAA
                     VMELVNEIYLVKGNLLAAGGDGAEAVRFATGTAASLIQPFAPHLAAEVFERVTGQRVW
                     EAAWPEADPDLLKSDTVTIVVQVGGKVRDRVEVADGAPEEEILATAKAAENVARHLEG
                     MTIVKEIVVPGKLVNLVVKPA"
     gene            1520..2161
                     /locus_tag="PLJ64_08265"
     CDS             1520..2161
                     /locus_tag="PLJ64_08265"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNC93515.1"
                     /translation="MPQINRQMLVYAVVGGVLLLVGFNSIRGEKSESASFGGDPAGGG
                     SRAESGADGGSGSGGFEVSGSSRKLIVDVAGAVRRPGVYRFSQGARVIDAIERAGGTT
                     RGAFVGAINRAATLSDGQQVIVPSATEAGAAGGTVSTGGTVSGTEAASAPVSLGTATQ
                     EQLEEIDGIGPVTAEKILEFRDAQGGLGSIDELDQISGIGPVTMESLRAALTP"
     gene            2158..4479
                     /locus_tag="PLJ64_08270"
     CDS             2158..4479
                     /locus_tag="PLJ64_08270"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HNC93516.1"
                     /translation="MNTAADWRLAAAIGGAAGLAASPFLGSGGSWLKPALAAALAIGA
                     FSHRGRLSGVGLLLVTLAAAIAGLLLGGARIDAIDAGALRAPAGATGLSGFVDSPPSH
                     SQGISRFDFETGEGKVLVETSSPTDGLLPGRGLEVSGELGPSPDWMAADLERRGIAMV
                     LRADRLRPDGLARGGVTGLIDRLRENAFEALAVAVPEQEAALSRGFVLGDDGEVDERT
                     TEDFRNSGLSHLLAVSGQNVVLLALLAIPFLSLIGARPRQRLLVIAGLILIYIPLAGG
                     GPSIQRAGVMGLAGLAALAATRAPSRAYALAIAALVTLGLNPRATGDIGWQLSFVAVI
                     GIMLLARPLQLRLEPVVGSEGWRRSLAEGIAVTLAATIVTAPLIAFHFERLPVGTLAA
                     NLVAMPAVAPAMWLGMISVAVGQLSPVLAAPFNLAGSLFLGWIAQVAEWFGRPEWAVI
                     EVSPGSALNLALISVALVLVIAAVLRFWRPGQAPVRPGRWLPAAALVLACLILLLPGL
                     TGSGRRHLDAPPPGGARIEILDVGQGDAILIRPSGADPILIDGGPPGGGISGALESAG
                     VSHLAAAILTHPHLDHYGGLLDLFGLIPVDRLLYDRAPAELLALAEDSGAELDRVNES
                     EEIRDGDLSLEILWPPAQGPTGEAAGVASGDQSAANENSIVTLLRWRGFRMLLTGDAE
                     EEAVPLDPGPIDVLKVAHHGSEDAGLPALLDESSPRLAVISVGEDNRYGHPAADALRE
                     LSADGVETLRTDRDGTVSIVLGAGPGYRVETGR"
     gene            4515..5474
                     /locus_tag="PLJ64_08275"
     CDS             4515..5474
                     /locus_tag="PLJ64_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93517.1"
                     /translation="MKPAYLIHGSDSAKIDQARTRLRQRAESESGAGGLEVFEPADGK
                     GSPDADALAASIGSLSLMPGRRYLLADRIEKWGKNQSATVAEALLAAPDETTVVLIAR
                     GKVPAGIAEAVKKVGGEDLSYEAPEGARLPVHLIESAAARGFELTPEAARFMIARMGE
                     SLPRLGNELDRLALWAGPDGLVDVEDLEEMVTDTSETSNFALGDAVISGDRREVIAVA
                     ERLLAQGVSAGSTVFSTSTSIRRAHKALVRLEAGEDPSRLERELGVPPFVARRLISSL
                     TGTTVESMREAAIAFAQLEIWTRGGAEYPDDLALDLALLAATA"
     gene            complement(5477..5815)
                     /gene="rpsT"
                     /locus_tag="PLJ64_08280"
     CDS             complement(5477..5815)
                     /gene="rpsT"
                     /locus_tag="PLJ64_08280"
                     /inference="COORDINATES: protein motif:HMM:NF013787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNC93518.1"
                     /translation="MACSSSLAYSQRPLVGSNTPASTPQIMANIASQKKRILRTERER
                     VENRRLTSAIKTYFNRLEKAADEGDADSVQAEHRALVSRIDKAVQKGAIHRNNGARKK
                     ARAARIASGS"
     gene            5803..7677
                     /gene="murJ"
                     /locus_tag="PLJ64_08285"
     CDS             5803..7677
                     /gene="murJ"
                     /locus_tag="PLJ64_08285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNC93519.1"
                     /translation="MNTPSDPQLDPFEAAARAAEEAIRNTSEKATRVMRQPVRNPDPV
                     PEPVREREDALVETASGLAEEGGAIAPVPVIEGTELDPEALAPTPAPEVKAPAPEPDG
                     ALKPEKPGRLARSTAFFSIATAASRVAGLVREIVAAGYFGISGPMSAFTVAFQVPNLI
                     RALFADAALQPAFVPIFTEQIEKGKYREAFRMASTLLLLVTMILGAITAFFILAAGLV
                     MPIFAPGFSAATEDLMIVLSQILFPILILLGVTGIVVGILNSYDRFGAFAISPFFWNI
                     TIIAVVIIGVPLFPEDKQIYAYAIGVVAGTLVQLLIPTFDLRNTPFQFTLKVDFRDPN
                     VRRVLIMMIPVTLSLGLINFNLLINSFFGSLVSDEAPAAIDKAFRIYQLPQGIFSVAI
                     ATVLFPTLARFANRKDFDSLRSTLAKGMRQIIFVLLPASAAILVLSEPVIRLVYQRGE
                     FNAAETEVVATALFWFAFSLPTNGMYLLLSRTFFGLQKPWIPTWIAGANLAVTAVGSF
                     LLYKPFGVAGIVAATAIATTVSVAIQTVMLRTRLAGIEFGRFLSSALRILIASAVLAG
                     VSYEIWDLLDEALGRGLAGQIISLGAGLGLGGISYAAVCQLLKVPELDQVAALLRRRS
                     "
     gene            7674..>8093
                     /locus_tag="PLJ64_08290"
     CDS             7674..>8093
                     /locus_tag="PLJ64_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93520.1"
                     /translation="MSFPAYLVGCLASLAGLAAVLVIAVVLGRCLLPGWRGPVAWVGA
                     AILAVASAIAPALALGTFGLLTGWSWLLLTLLLAAALWRWQERLPAARARPALISEPA
                     SPGLTMVGTAVAAGAFGAFVAGIAIRLGTGMTGFDSTW"
BASE COUNT         1190 a         2723 c         2802 g         1378 t
ORIGIN      
        1 ccggtgaagc gggtgatcga gggcgagccg gaggaggggg gagacgaccg gggcctgccc
       61 tatgcgggcg acggtccgat gaccggctcc ggccgtttcg acggcatgaa caaccgggcg
      121 gcctacgacg agatcgtcga gtggctgaag ctcgaaggcc ggggcgacgc ctcggtcaac
      181 ttccgcctgc gtgactggct cgtgtcgcgg cagcggtact ggggcgcgcc gatcccgatc
      241 gtctactgcg atgaatgcgg catggtcccg gtgcccgaag agcagttgcc ggtccggttg
      301 ccggagatcg acgactacgc gccgaagggc aagagtcccc tggccgcggc gacgcactgg
      361 gtcgccacca cctgcccgaa gtgcggggcc gccgccgagc gcgaaaccga caccatggac
      421 accttcgtcg attcgtcctg gtacttcctg cgctatctgg acgcgcagaa ctccgaggcc
      481 gcctgggatc gggaagccgc ggatttctgg atgccggtcg accagtacat cggcggagtc
      541 gagcacgcga tcctccacct gatgtacgcc cgcttcttcg ccaaggcgct caaggacatg
      601 gggcacatcg gtgtggtcga gcccttcgcc aacctcttca cgcaggggat gatcactcgc
      661 gacggtgcca agatgtcgaa gtcgaagggc aacacggtca gtccttccga gatcgtcgcc
      721 cgccacgggg ccgacgccgc ccggacctac gtctgcttca tgggcccgcc ggagcgttcc
      781 ggcgactgga ccgacgaagg ggtggagggc gtccaccgtt tcctggccag gctctggcga
      841 ctcggcgagg agctggctgc cgccggccag tccgggccac tggcgttgcc ggacgggctc
      901 gacggcgagg cccgcgaggt tgcggccaag gcccactggg cgatcgacaa ggcgaccggt
      961 gacttcaacc gcggcttcca gttcaacacg gtcatcgccg ccgtgatgga gctggtaaac
     1021 gagatctacc tggtcaaggg gaacctgctc gcggccggcg gcgacggagc ggaggcagtg
     1081 cgtttcgcga ccgggaccgc agcctccctg atccagccct tcgcgcccca tctggccgcc
     1141 gaagtcttcg aacgagtgac cggccagagg gtctgggaag ctgcctggcc cgaagccgat
     1201 ccggacctgc tcaagtccga cacggtgacc atcgtcgtcc aggtcggagg caaggtgcgc
     1261 gaccgggtcg aggtcgccga cggtgccccc gaagaagaga tcctcgcgac ggccaaagcc
     1321 gccgaaaacg tcgcccgcca cctcgaagga atgaccatcg tcaaggagat cgtcgtcccc
     1381 ggcaagctgg tcaacctggt cgtcaaaccg gcctgaagta gcccgaaacc gtcggagtga
     1441 gccggaaacg gggctccacg ttactgaatc gttgcacctg tagagccacg tgtgaggttt
     1501 ttcgtggcag tttcggggca tgccgcaaat caaccgacag atgctcgttt acgccgtggt
     1561 cggcggcgtg ctgctgctgg tcggcttcaa ctcgatccgg ggggagaagt ccgagtcggc
     1621 cagcttcggc ggggatcccg cgggtggcgg gtccagggcg gaatcgggag cggatggcgg
     1681 ctccggaagc ggcggcttcg aggtcagtgg cagtagccgc aaactgatcg tcgacgtggc
     1741 cggggcggtg cggagacccg gggtctaccg gttctcccag ggtgcccggg tgatcgacgc
     1801 gatcgagcgg gccggtggca ccacacgcgg ggccttcgtc ggggcgatca accgggcggc
     1861 gaccctgagc gacggccagc aggtgatcgt gccctcagcg acggaggccg gcgcagccgg
     1921 tggaacggtt tcgaccggcg ggaccgtttc cggaaccgaa gcagctagcg ccccggtcag
     1981 cctcggaacg gcgacccagg agcagctcga ggagatcgac gggatcgggc cggtgaccgc
     2041 ggagaagatc ctcgagtttc gggatgcaca gggtgggctc ggatcgatcg acgagctcga
     2101 ccagatcagc ggcatcgggc cggtaaccat ggagtcgctc cgggcggccc tcacgccgtg
     2161 aacaccgccg ccgactggcg gctcgcggcg gcgatcggcg gcgcggcggg gctggccgcc
     2221 tcgccctttc tgggatcggg cggcagttgg ttgaagcccg cccttgcggc ggcactcgca
     2281 atcggtgcgt tctctcaccg ggggcggttg agcggagtcg gcctgctgct ggtcactctg
     2341 gccgcggcaa tcgccggact cctgctgggc ggggcacgga tcgatgcgat cgacgccggt
     2401 gccctgaggg cgccggccgg cgcgaccgga ctctccgggt ttgtcgattc gccgccttct
     2461 cactcgcagg ggatctcccg cttcgatttc gagaccggtg aaggcaaggt gctggtcgaa
     2521 acctcctcac cgaccgacgg cctcctgccc ggccggggcc tcgaggtcag cggagaactc
     2581 ggcccgtccc ccgactggat ggcggccgac ctcgagcggc gcggcatcgc catggttctg
     2641 cgagccgacc ggctgcggcc ggacggactc gcacggggcg gcgtgaccgg tctgatcgac
     2701 cggttgcggg agaacgcctt cgaggcgctg gcggtcgcgg tgccggagca ggaggcggca
     2761 ctctcgcggg ggttcgtgct tggtgacgac ggcgaggtcg acgagcggac gaccgaggat
     2821 ttccgcaact cggggttgag tcacctgctc gcggtgtccg gccagaacgt cgtgctcctc
     2881 gccctgctgg cgattccgtt cctctccctg atcggcgccc gcccccggca gcggctcctg
     2941 gtgatcgcgg ggctcatcct gatctacatc ccgctggccg gtggcgggcc gtcgatccag
     3001 cgggcaggag tgatggggct ggccggtctc gccgcgctgg cagccaccag ggccccatcg
     3061 cgggcctatg cgctggcgat tgccgccctg gtcacgctcg gactgaatcc gagggcgacc
     3121 ggagacatcg gctggcagct cagctttgtt gcggtgatcg ggatcatgtt gctcgcccgg
     3181 ccgcttcagt tgcggctgga accggtggtc ggttcggaag gctggcggcg cagcctggcc
     3241 gaggggatcg cggtaaccct ggcggcgacg atcgttacgg cgccgctgat cgccttccac
     3301 ttcgagcggc tgccggtcgg gacgctcgcc gcaaacctgg tggccatgcc ggcggtcgct
     3361 ccggccatgt ggctgggcat gatttcggtc gcggtcgggc agctcagccc ggtgctcgcg
     3421 gcgccgttca atctggccgg ttccctcttc ctcggctgga tcgcccaggt agccgagtgg
     3481 ttcggacggc cggaatgggc ggtgatcgag gtgagccccg gcagtgcctt gaacctggcc
     3541 ctgatctcgg tggcgctggt tctggtcatc gcggccgtcc tgcgcttctg gcggccgggg
     3601 caggcgccgg tccggcccgg ccgctggctt cccgcggcgg cgctggtcct cgcttgcctg
     3661 attctgctcc tgccgggcct gaccggaagc gggcgccggc atcttgacgc gccgccgccg
     3721 ggcggtgcca gaatcgaaat cctcgacgtg ggtcagggag acgcgatcct gatccgaccc
     3781 tccggcgccg acccgattct gatcgatggc ggtccgcccg gcggaggcat ctccggggct
     3841 ctcgaatcag ccggggtaag ccacctcgcc gcggcgatcc tgacccaccc gcatctcgac
     3901 cactacggcg ggctgcttga tctctttggc ctgatcccgg tcgaccggct gctctacgac
     3961 cgcgcaccgg cggaactgct cgccctggcg gaagattccg gcgccgagct cgaccgggtg
     4021 aacgagtcgg aggaaatcag agacggtgac ctgagccttg agatcctctg gccacccgct
     4081 cagggaccta ccggcgaagc tgccggcgtg gcctccggcg accaatccgc ggccaacgag
     4141 aactcgatcg tcaccctgct ccgctggcgg ggctttcgca tgctgctgac cggcgacgcc
     4201 gaggaggaag cggtgccgct cgatcccggg ccgatcgacg tgctgaaggt tgcccatcac
     4261 ggcagcgagg atgcgggact gccggcgctc ctcgatgaat cctcgccccg gctcgccgtg
     4321 atctcggtcg gcgaggacaa ccgctacggc cacccggccg cggacgcgct gcgtgaactt
     4381 tccgcggacg gcgtcgagac cctgcgcacc gaccgggacg gcaccgtgtc gatcgtactc
     4441 ggcgccggcc cgggctaccg ggtggaaacg gggcggtgag ggttgaacgc cgggtcatcc
     4501 gggacaatga aggggtgaag cccgcctacc tcatccatgg cagcgacagc gccaagatcg
     4561 accaggcccg cacccggttg cggcaacggg ccgagtccga gagcggtgcc ggtggtctgg
     4621 aggtcttcga accggccgac ggcaagggaa gccccgacgc cgatgcgctt gccgcctcga
     4681 tcggttcgct ctcgctgatg ccgggccggc gatacctgct cgccgaccgg atcgagaaat
     4741 ggggcaagaa ccagtccgcg acggtcgccg aggcgctgct ggccgcgccc gacgagacga
     4801 cggtggtact tatcgcgcgg ggcaaggttc cggccgggat cgcggaagcc gtgaagaagg
     4861 tcgggggcga ggatctttcc tatgaagctc cggaaggcgc ccggctgccg gtgcacctga
     4921 tcgagagtgc agcggcccgg ggattcgagc tgaccccgga ggccgcccgc ttcatgatcg
     4981 cccggatggg ggagagcctg ccccggctcg ggaacgaact tgaccggctg gcgctctggg
     5041 ccggccccga cgggctggtc gacgtcgagg acctcgaaga gatggtcacc gacacatccg
     5101 aaaccagcaa cttcgctctc ggcgatgcgg taatcagcgg ggaccggcgg gaagtgatcg
     5161 ccgtcgccga gcgcctgctc gcccagggtg tgagtgcggg cagcaccgtc ttctcgactt
     5221 cgacctcgat ccgccgggct cacaaggcac tggtcaggct ggaagccggg gaggatccgt
     5281 cacgactcga acgggaactc ggggtgccgc cgtttgtcgc cagacggctg atcagctcgt
     5341 tgaccggaac cacggtcgaa tcgatgcggg aggcggcgat tgccttcgca caactggaga
     5401 tctggacccg tggcggcgcc gaatacccgg acgacctcgc cctcgacctc gccctcctgg
     5461 ccgccaccgc gtgaagctag gagccggagg cgatacgggc agcgcgcgct tttttcctcg
     5521 cgccgttgtt gcggtggatc gcgcccttct gaaccgcctt gtctatccgc gagaccagag
     5581 cgcgatgctc ggcctggacg gagtcggcgt cgccttcatc ggcggccttc tcgaggcggt
     5641 tgaagtaagt cttgatcgca gaggtaagcc gccggttttc gacgcgctcg cgctcggtgc
     5701 gaaggattct ctttttctgt gaggctatgt tggccatgat ttgtggtgtg cttgcgggcg
     5761 tattgctgcc gacgagcggc cgctgactat acgccaaaga gcttgaacac gccatccgac
     5821 ccccaactcg acccctttga agctgccgcc cgcgcggctg aagaggcgat ccgcaacacc
     5881 tcggagaagg ccacccgggt gatgcggcag ccggtgagga atccggatcc cgtccctgaa
     5941 ccggtgcggg aaagggaaga cgcactggtt gaaaccgcat ccgggctggc cgaggagggg
     6001 ggagccattg ccccggtccc ggtgatcgag ggaacggaac tcgaccccga ggccctggct
     6061 ccgacgccgg caccggaggt gaaggcgccc gcccccgaac cggacggggc cctcaaaccg
     6121 gaaaagccgg gacgcctggc ccgctcgacc gccttcttct cgatcgccac cgccgcctcc
     6181 cgggtggccg ggctggttcg cgaaatcgtc gccgcagggt atttcggcat ctcgggaccg
     6241 atgtcggcct tcaccgtcgc cttccaggtt ccgaatctga tccgggcact tttcgccgat
     6301 gccgcgctgc agcccgcctt cgtcccgatc ttcaccgagc agatcgagaa gggcaagtac
     6361 cgggaggcct tccgcatggc ctcgaccctg ctcctgctgg tgaccatgat cctcggcgcg
     6421 atcaccgcct tcttcatcct cgccgccggc ctggtcatgc cgatcttcgc tcccggcttc
     6481 tcggccgcca ccgaagacct gatgatcgtg ctttcgcaga tcctgttccc gatcctgatc
     6541 ctgctcgggg tgaccgggat cgtggtcggc atcctcaaca gctacgaccg cttcggcgcc
     6601 ttcgcgatat cgccgttctt ctggaacatc acgatcatcg cggtggtcat catcggggtg
     6661 ccgctcttcc ccgaggacaa gcagatctac gcgtacgcga tcggtgtcgt ggcgggcacc
     6721 ctggtccagc tcctgatccc caccttcgac ctccggaaca cgcccttcca gttcaccctc
     6781 aaggtcgatt tcagggatcc gaacgtaaga cgggtgctga tcatgatgat cccggtgacg
     6841 ctcagcctcg gcctgatcaa cttcaacctg ctgatcaaca gcttcttcgg ctcgctggtc
     6901 agtgacgagg ccccggcggc aatcgacaag gccttccgga tttatcagct cccgcagggc
     6961 atcttctcgg tggcgattgc gacggtcctc ttcccgaccc tggcccgttt tgccaaccgc
     7021 aaggacttcg actcgctgcg gagcacactg gccaagggca tgcggcagat catcttcgtg
     7081 ctcctgccgg ccagcgccgc gatcctcgtg ctctccgaac cggtgatccg cctcgtctac
     7141 cagcgaggcg agttcaacgc agccgagacc gaggtcgttg cgaccgcgct cttctggttc
     7201 gccttctcgc tgccgaccaa cggcatgtac ctgctgctca gccgaacctt cttcgggctc
     7261 cagaagccct ggatcccgac ctggattgcg ggcgccaatc tcgccgtgac cgcagtcggt
     7321 tccttcctgc tctacaagcc tttcggggtg gccgggatcg tggcggcgac cgcgatcgcc
     7381 acaaccgttt cggtggcgat ccagacggtc atgcttcgca cccgtttggc cgggatcgag
     7441 ttcggccggt tcctctcgtc ggccctgcga atactgattg cctcggccgt gctggccggg
     7501 gtctcctacg agatctggga cctgctcgac gaggcccttg gtcgcgggct cgccggccag
     7561 atcatttcgc tgggcgccgg gctcgggctg gggggcatct cctatgccgc ggtctgccag
     7621 ctgctgaagg tccctgaact cgatcaggtc gctgcccttc tgcggcgccg gtcgtgagct
     7681 tccccgcgta cctggtcggc tgcctcgcca gtctcgccgg gctggccgcc gtactggtta
     7741 tcgcggtcgt cctcggccgg tgcctgctgc ccggctggcg cgggcccgtg gcctgggtcg
     7801 gggcggcgat cctggcggtg gcttcggcga tcgctccggc cctggccctc ggcaccttcg
     7861 ggctgctgac cggctggagc tggctgctcc tgaccctcct tctggcggcg gcgctctggc
     7921 gctggcagga gcggctgcct gcggcccggg cccggccggc cctgatttcc gaacctgctt
     7981 ctcccggatt gaccatggtc ggtaccgccg tcgcggccgg cgccttcggc gccttcgtgg
     8041 ccggcatcgc gatccggctg ggcaccggca tgaccggatt cgattcgacc tgg
//
LOCUS       DAOQKN010000114         8034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_4364_length_8034_cov_6.134729, whole genome shotgun sequence.
ACCESSION   DAOQKN010000114 DAOQKN010000000
VERSION     DAOQKN010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 8034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8034
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4364_length_8034_cov_6.134729"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            312..965
                     /locus_tag="PLJ64_08295"
     CDS             312..965
                     /locus_tag="PLJ64_08295"
                     /inference="COORDINATES: protein motif:HMM:NF025691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HNC93521.1"
                     /translation="MTDTPTPTPGGEEEIVVSEVSESVVVPGGYPVDVDAQLLPEYSR
                     FMPLIKWLILLPHYIVLFFLGIGAMFVAFIAFFATLFTAKYPEGMWNYMVGVHRWALR
                     VMAYNFLITDNYPPFTLQETDEDTIQLVAEYPERVSRWRPFFAGLICIPYAIVASLIY
                     MVASICSFFAFFTILFTKKIPAGLFNVIRNGFTWNLRAGFYSYWMSTEYPPFEWDED"
     gene            complement(1548..2225)
                     /locus_tag="PLJ64_08300"
     CDS             complement(1548..2225)
                     /locus_tag="PLJ64_08300"
                     /inference="COORDINATES: protein motif:HMM:NF025691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HNC93522.1"
                     /translation="MYPVTYEADYLRERNRLTTFFRLIVAIPWLIVAYIYVIAQMVVV
                     FIAWFAILFVGRFPEGMYNFVGGILRFTQRVNGFMYLQTDAWPPFGIGDDDSYPIRVK
                     FHPPAARQSRMKDLFRLILAIPLLVLSYAMSYALMAVALISWLTIVFRGYQPAAIHNA
                     IAYLMGWSTRASAYIYLMRDEYPPVGDETPVEVDPPAGALPASDAAPLDPAPVSEEQP
                     PPPPPAQ"
     gene            2368..3498
                     /gene="serC"
                     /locus_tag="PLJ64_08305"
     CDS             2368..3498
                     /gene="serC"
                     /locus_tag="PLJ64_08305"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HNC93523.1"
                     /translation="MTDFAKIPDDLKPADGRFGCGPSKVRPEALESLAERSDLMGTSH
                     RQKPVKDLVGRVRSGLGELFSIPDGYEIALGNGGATAFWDAAAAWLVRDRALHLVYGE
                     FSRKFSQVTAGAPFLSDPVLIEAEPGSAPAPESRDGVDVIGWAQNETSTGVMVPVERP
                     AGADDALVLIDATSGAGGLPVALEETDVYYFAPQKAFGSDGGLWLAALSPAAIARIEE
                     LDGTSGRWQPPFLSLKTALDNSRKDQTYNTPALATLYLLAEQLDWMLAGGGLEFCVGR
                     TRASSSHLYGWAEASDFATPFVTDPALRSMVVGTIDFDDSIDAAAVAATLRANGIVDV
                     EPYRKLGRNQLRIAMFPAVDPADVEALTACVDWVIGNAGVTA"
     gene            3551..5143
                     /gene="serA"
                     /locus_tag="PLJ64_08310"
     CDS             3551..5143
                     /gene="serA"
                     /locus_tag="PLJ64_08310"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HNC93524.1"
                     /translation="MSERAKVLVKEKIADSGVEQLKENFDVEIGVDMDDAELAERIGE
                     FDAILIRSATKLTPELIEKADNMKVIGRAGTGVDNVDIPAATKRGIIVANAPESNSVA
                     AAEHTMALALALFRNVPQAHSALVDGRWDRAKYKGAELYGKTLGVIGFGRIGQLVAKF
                     AQGFEMEVLAFDKFVSHERFRDLGVEGVDSAEEIYRRADLITIHLPKTPETVDWIDAE
                     AIARFKPGMRIVNCARGELVNLDALIDGLESGQLAGAALDVFPEEPFTEHPIFERDDV
                     VVTPHLGASTAEAQDRAGTDTAEQVTAALTGGVVTNAVNIPAVSPAAMEAVAPYVPLC
                     ETLGKVAVGLTPGSIDRVEVEFRGGIAENDTRLLGIAVQAGLLSGHTEEAVNLVNAPQ
                     MAEERGIEVIEIKDTTADEFTDLVRVTIQSGADVVTVGGTTVGPKNAPYLVSIWGQGF
                     YLPFAEHMAVFRYADQPGMIGKVGSLFGEEGVNIGSAAVGAEADSDNAVMVVTSDAPV
                     SAQTIETIKAVDGFSAGYAIEL"
     gene            5175..6203
                     /locus_tag="PLJ64_08315"
     CDS             5175..6203
                     /locus_tag="PLJ64_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNC93525.1"
                     /translation="MRAVVLTGHGGPDDLKVQERPDPPAGPGEVRVAVKAAGVNFADV
                     MATRGLYPDAPKPPCVIGYEFAGEVESVGPGVEGVATGDQVFGMTMFGGYAELVTVPS
                     DDVFPIPDGLSLEQAAALPVNYATALAALVVMGGLSEGQRVLIHAAAGGVGIAATQIA
                     RQAGAEIFGTASAAKHEVIRDQGVEHPIDYRNLDFEQEVRRLTGGEGVDLIIDATGPT
                     NFRKDYRLLRPGGKMVMYGASEVTSGKGMISRQSLSALVRMPLVATPWWKSFGVMNEN
                     KGVFGLNLLDWWKDEGLVRLGALLEAGLERGDFTPVVDRSFGFEEAGDAHRFIADRQN
                     VGKVLLEP"
     gene            6405..7988
                     /locus_tag="PLJ64_08320"
     CDS             6405..7988
                     /locus_tag="PLJ64_08320"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNC93526.1"
                     /translation="MSSQDTDVNRVQIFDTTLRDGEQSPGISLNANEKIEIAQQLARL
                     GVDVIEAGFPIASPGDFEAVQRIAREVHGPTICGLARAQAPDIDAAWNAIRDSEQPRI
                     HTFISTSDIHIQHQMRNTREDVLKGARAAVAQARSYCEDVEFSPMDATRSDVEFTAEV
                     CAIAVEEGATVVNIPDTVGYTTPAEYTKFFEDLYRFAPSLKEVRLSVHCHDDLGLAVA
                     NSYAGVLAGARQVECAINGIGERAGNCSLEEIAMLIKVREDVHGLHTNINSKELARAS
                     RMVSRLTGYVVQPNKAVVGRNAFAHESGIHQDGVLKERETFEIMDATEVGFDSNQLVL
                     GKHSGRHALKDALEQLGFVVDGEALNQAFKRFKDVADKKKQVTALDLEAIVSDEMRER
                     PQSHELEWFEAEAGSERKPVGRVRIKHPSGEVTSGESTGDGPVEAVFAAILAATGAEA
                     ELREYHVGAVTGGEDALGQVTVVLKNENGTTSGQGVSTDIIEASARAYVRALGNLLEG
                     SAIRQAEEATSEAVKDHAP"
BASE COUNT         1406 a         2573 c         2650 g         1405 t
ORIGIN      
        1 tgacagctca cgtgcccgcg cactatcgcg gtcatttccg gcctgtctcc tcgccagcga
       61 acggctggag ggctctgacc tccgcgtccc ggctttcctt cttggcagcg aacggctggc
      121 ggcccctgaa ctttgcgctt tcaaagtctc gttctcgcca gcgaacggct ggagggctct
      181 gacctccgcg tccctggggc ttcgtgcccg gattcgttgg gattttcggg gcgtttacag
      241 gagaacgccg cttcagcgag taggtttccc gtggccccgg tcccggggga agacctttag
      301 aaggagtcgt gatgactgat acaccaaccc ccacacctgg cggcgaagaa gagatcgtcg
      361 tttccgaggt gagcgagtcc gtcgtcgtcc cgggtggcta cccggtggac gtggacgccc
      421 agcttctgcc cgagtactcg cggttcatgc cgctgatcaa gtggctgatc ctgctgcccc
      481 actacatcgt gctcttcttc ctcgggatcg gcgccatgtt cgtggccttc atcgccttct
      541 tcgccaccct gtttacggcg aagtacccgg aaggcatgtg gaactacatg gtcggcgttc
      601 accgttgggc cctgcgagtg atggcctaca acttcctgat caccgacaac tacccgccgt
      661 tcacgctcca ggaaaccgac gaggacacga ttcagcttgt ggccgaatac ccggagcggg
      721 tctcgcgctg gcgtcccttc ttcgccgggc tgatctgcat tccgtacgcg atcgtcgcct
      781 cgctgatcta catggttgcg agcatctgct ccttcttcgc tttcttcacg atcctcttca
      841 ccaagaagat ccccgctggc ctcttcaacg tgatccgcaa cggattcacc tggaatctcc
      901 gggccggctt ctactcctac tggatgtcca cggagtaccc gcccttcgag tgggacgagg
      961 actgaccctc gccgctccag cggtaatcag ttacaccgaa agggcggcct ccgggccgcc
     1021 ctttcttgtg gttgaagggt ctggcccggg cggcgcccgg aaccaccagg acgacgctga
     1081 ccttcgtcaa cggccggaaa cggcagctca agtacccacg tacgatcgcg gccatttgcg
     1141 gccttattcc tcagcagccc cgccctggag gcccctggca tccacccgct gaagttgggt
     1201 ggcgttggca tgtcgcgaaa ggcagttttc tgccgtatac cggcagaaac ttgcctctcg
     1261 ccgtcttccc gaccccgttc cggggctggt gcctatggat ttcgttcata tagacgaaat
     1321 ccatacccgc ctcccgaaac cggggcctga aaccggccct ggaggtgcca cgggacgagg
     1381 gaagccccgc gatttccccg tcacctgccc tgttccacca actgggtcgg aatctcggca
     1441 gtcgggccgg aaatccgacc ccgcgtcctc caaaccaggg tggtgcaaag aaaacgggcg
     1501 gctctgaggc cgcccgtttt cggatttctg tcgcctctcg gcccgtgtta ctgggccggg
     1561 ggaggaggcg ggggctgctc ctcggacacc ggggcgggat cgagcggggc ggcgtcgctg
     1621 gcgggaagag cgccggcggg cggatcgacc tcgaccgggg tctcgtcacc gaccggcggg
     1681 tactcgtcgc gcatcaggta gatgtacgcg gaagcccggg ttgaccagcc catcaggtag
     1741 gcaatcgcgt tgtggatcgc cgccggctgg taaccgcgga aaacgatggt caaccaggag
     1801 atcagcgcga cggccatcag ggcgtagctc atggcgtagg agagaacgag cagcgggatc
     1861 gccaggatga gccggaacag gtccttcatg cggctttgcc ttgcggcggg gggatggaac
     1921 ttgacccgga tcgggtacga gtcgtcgtcg ccgatgccga agggcggcca ggcgtcggtc
     1981 tgcaggtaca tgaagccgtt gacccgttgc gtgaagcgga gaatcccgcc cacgaagttg
     2041 tacatcccct ccgggaaccg cccgacgaag aggatcgcga accaggcgat gaaaaccacc
     2101 accatctggg cgatcacgta gatgtaggcc acgatcagcc acggaatcgc gacgatcagg
     2161 cggaagaagg tggtgagccg gttccgttct cgcagatagt cggcttcata tgtgacggga
     2221 tacaagattc tctccttggg ttcgggggga ctggtcgaac tttcattcag actatccgtc
     2281 ctcccggacc tcgtgaagat gcatttgcga caggccgtcc aggggcggga atctgtcgag
     2341 ctcggcgact caatatcctc acccgagatg actgatttcg ccaagatccc ggatgacctg
     2401 aagcccgccg acggccgctt cggctgtggc ccctcgaagg tgcgacccga agcccttgag
     2461 tcgctggccg agcgatcgga cctgatgggt acctcccatc ggcagaagcc ggtcaaggat
     2521 ctggtcgggc gcgtccgcag cggcctcgga gagctctttt cgattcccga tggttatgaa
     2581 atcgccctcg gaaacggcgg ggcaaccgca ttctgggatg ccgcggcggc gtggctggtc
     2641 cgggaccggg cgctccacct ggtctatggc gagttttccc gcaagttctc gcaggtcacg
     2701 gccggggccc cgtttttgtc cgaccccgtc ctgatcgaag ccgagccggg cagcgccccc
     2761 gcgcccgaaa gccgtgacgg cgtcgacgtg atcggctggg cccagaacga gacctcgacc
     2821 ggagtcatgg ttcccgtcga gcgtcccgcc ggcgccgacg acgccctggt tctgatcgac
     2881 gcgacctccg gtgccggtgg ccttccggtc gccctcgagg agaccgacgt ctactacttc
     2941 gccccccaga aggcgttcgg ttccgacggc ggtctctggc tggccgcgct gagccctgcc
     3001 gccattgccc ggatcgagga gctggacggt acatccggcc gctggcagcc gcccttcctg
     3061 tcgctgaaga cggcgctcga taactcccgc aaggatcaga cttacaacac ccccgcgctt
     3121 gccacccttt acctgctggc cgagcagctc gactggatgc tcgcgggcgg gggcctcgaa
     3181 ttctgcgtcg ggcggacccg cgcttcgtcc tcgcaccttt acgggtgggc cgaggccagt
     3241 gatttcgcga ctcccttcgt gaccgacccg gcgctgcggt caatggtcgt cggaacgatc
     3301 gatttcgacg actcgatcga cgcggccgcg gtcgcggcga cgctgcgggc caacggcatc
     3361 gttgacgtcg agccctaccg caagctcggc cggaaccagt tgcggatcgc gatgttcccg
     3421 gcggtcgacc ccgctgacgt cgaggcgctc accgcctgcg tcgactgggt gatcgggaac
     3481 gccggggtca ccgcgtgaag agacttgacc cacaccggtt ctcccggacc ggccaaccag
     3541 gaggattgat ttgagcgagc gagccaaggt actcgtcaag gagaagatcg ccgacagcgg
     3601 cgtcgagcag ctgaaggaga acttcgacgt cgagatcggc gtcgacatgg atgacgcgga
     3661 actggccgaa cggatcggcg agttcgacgc gatcctgatc aggtcggcca ccaagctcac
     3721 tcccgagttg atcgagaagg ccgacaacat gaaggtgatc ggccgggccg gcaccggggt
     3781 cgacaacgtc gacatcccgg ccgcgaccaa gcggggcatc atcgttgcca acgcccccga
     3841 atcgaattcg gtcgcggcgg ccgagcacac gatggctctc gccctggccc tgttccgcaa
     3901 cgtgccgcag gcccattccg ccctcgttga cggccgctgg gaccgggcaa agtacaaggg
     3961 cgccgagctc tacggcaaga ccctgggcgt gatcggcttc ggccggatcg gccagttggt
     4021 cgccaagttc gcgcagggat tcgagatgga ggttctcgcc ttcgacaagt tcgtctccca
     4081 cgagcggttt cgcgatctcg gcgtcgaggg ggtcgactcg gccgaggaga tctaccgccg
     4141 tgcggacctg atcacgatcc acctgccgaa gaccccggag accgtcgact ggattgacgc
     4201 cgaggcgatc gccaggttca agcccggcat gcggatcgtc aattgtgccc gcggtgagct
     4261 ggtcaatctc gatgcgctga tcgacggcct cgaatcgggt cagctggccg gggccgccct
     4321 ggacgtgttc ccggaggagc cgttcaccga gcatccgatc ttcgagcggg atgacgtggt
     4381 cgtgacgccg catctcggcg cttcgaccgc cgaggcccag gaccgggccg gtaccgacac
     4441 ggccgagcag gtgaccgccg ccctgaccgg cggggtggtg accaacgcgg tcaacattcc
     4501 ggctgtgagt ccggccgcga tggaagcggt tgcgccctat gtgccgctct gcgagacgct
     4561 cggaaaggtc gcggtcggcc tgaccccggg ttcgattgac cgggtcgagg tcgagttccg
     4621 cggcggcatc gccgagaacg acacccggct gctcgggatc gcggttcagg ccggtcttct
     4681 ttccggccac accgaggagg cagtgaacct ggtcaacgct ccgcagatgg ccgaggaacg
     4741 cggcatcgag gtgatcgaga tcaaggacac cacggccgac gagttcacgg acctggtccg
     4801 ggtgacgatc cagtcgggag ccgatgtcgt aaccgtcgga ggaaccacgg tgggcccgaa
     4861 gaacgcgcct tatctcgttt cgatctgggg tcagggcttc tacctgccgt tcgccgagca
     4921 catggccgtc ttccgctacg ccgaccagcc ggggatgatc gggaaggtcg gatcactgtt
     4981 cggcgaagaa ggcgtcaaca tcggttcggc cgcggtcggt gcggaggcag acagcgacaa
     5041 cgcggtgatg gtcgtgacct ccgatgcccc ggtttcggcg caaacgatcg aaacgatcaa
     5101 ggcagtcgac ggtttctcgg ccggatacgc gatcgagctc tgatcaaccc ggcttccgcg
     5161 gataggctgg agccatgaga gctgtcgtcc tgaccggaca cgggggaccg gatgacctga
     5221 aggttcagga gcgtccggac ccgccggccg gtccgggcga ggtgcgggtc gcggtcaagg
     5281 cggccggagt caacttcgcg gacgtgatgg caacccgggg tctttacccg gacgcgccca
     5341 agccgccctg cgtgatcgga tacgagttcg ccggggaggt cgaatcggtc gggcccggcg
     5401 ttgaaggggt cgcaaccgga gaccaggtat tcggcatgac catgttcggc ggatacgcag
     5461 aactggtcac cgtcccgtcc gatgacgtct ttccgattcc cgacggtttg tcgctggagc
     5521 aggcggcggc gctgccggtc aactacgcga ccgccctcgc cgctctcgtc gtgatgggcg
     5581 gcctgagcga gggccagcgg gtgctgattc acgcggcggc cggaggcgtc gggatcgccg
     5641 cgacccagat cgcccgccag gccggagccg agatcttcgg caccgcctct gccgccaaac
     5701 acgaggtgat ccgggaccag ggagtggagc atccgattga ttaccgcaac ctcgacttcg
     5761 aacaggaggt ccggcggctg accgggggag agggcgtcga tctgatcatc gacgccaccg
     5821 gcccgaccaa cttccgcaag gattaccggc tgctgcggcc cggcggcaag atggtgatgt
     5881 acggggcctc cgaagtgacc tccggcaagg ggatgatcag ccggcagtcg ctctcggctc
     5941 tggtcaggat gccgctcgtg gccacgccct ggtggaagag cttcggggtg atgaacgaga
     6001 acaagggcgt cttcggtctc aacctgctcg actggtggaa ggacgagggc ctggtcaggc
     6061 tgggggccct gctcgaggct ggactggagc ggggagactt caccccggtg gtcgaccgct
     6121 ccttcggttt tgaggaagcc ggtgatgctc accgcttcat cgccgaccgc cagaacgtcg
     6181 gcaaggttct gctcgagcct tgaaacctgc cagtttcctt gccagggccg gttccttgaa
     6241 gtcgccccgc tccttgcgct acgcttgacc ctcggtatga atcaccgttt caacagcccg
     6301 gcccttcttc tccttctccc cctctggggg gactgatcgc cgggcggcgc gatagccgcg
     6361 agctgaaagc aagattcgag ttacttagct agaaggagaa ggacatgtcc agtcaggaca
     6421 cagacgtaaa ccgcgttcag atatttgaca cgaccctgag agacggggag cagtctccgg
     6481 gaatctcgtt gaacgcgaac gagaagatcg agatcgccca gcagctggcg cggctcggcg
     6541 tagacgtgat cgaagccggc ttcccgatcg cttcgcccgg tgacttcgag gcggtccagc
     6601 ggatcgcccg tgaggttcac ggcccgacca tctgtggcct ggctcgcgcc caggccccgg
     6661 acatcgacgc cgcctggaac gcgatccgcg attccgagca gccccggatc cacaccttca
     6721 tttccacctc tgacatccac atccagcatc agatgcggaa caccagggag gacgtgctga
     6781 agggagcccg ggccgccgtc gcccaggcca ggtcctactg tgaggatgtc gaattctcgc
     6841 cgatggatgc gacccgcagt gatgtcgaat tcacggccga ggtctgtgcg atcgcggtcg
     6901 aggagggtgc caccgtggtc aacattcccg acacggtcgg ttacaccacg cccgccgagt
     6961 acacgaagtt cttcgaggac ctctaccgct tcgccccttc gctgaaggaa gtgaggctgt
     7021 cggtccactg ccacgacgat ctgggcctcg cggtcgccaa ctcgtatgcc ggcgtgctgg
     7081 ccggcgcccg ccaggttgaa tgtgcgatca acgggatcgg cgagcgagcc ggcaactgct
     7141 cgctggaaga gatcgcgatg ctgatcaagg tgcgcgagga cgttcacggc ctgcacacca
     7201 acatcaactc gaaagaactg gcccgggcca gccggatggt ttcccgcctg accggctacg
     7261 tggtgcagcc gaacaaggcc gtggttggca ggaacgcctt cgcccatgag tccggcatcc
     7321 atcaggacgg cgtgctgaag gaacgcgaaa ccttcgagat catggacgcg accgaggtcg
     7381 gtttcgactc gaaccagctg gtgctgggca agcattccgg ccggcatgcg ctgaaggacg
     7441 cgctggaaca gctgggtttc gtggttgacg gcgaggccct gaaccaggct ttcaagcggt
     7501 tcaaggacgt ggccgacaag aagaagcagg tcacagcgct ggatctggag gcgatcgttt
     7561 ccgacgagat gcgcgaacgg ccccagtccc atgagctgga atggttcgaa gccgaggccg
     7621 gcagcgaacg caagccggtc ggccgggtgc ggatcaagca tccctcgggc gaggtcacct
     7681 cgggcgaaag caccggtgac ggtccggtcg aggcggtctt cgcggcgatc ctggctgcca
     7741 ccggcgccga agcggagttg cgcgagtacc acgtcggtgc ggtgaccggt ggcgaagacg
     7801 ctctgggcca ggtgaccgtg gtgctcaaga acgagaacgg caccacatcc gggcaggggg
     7861 tttcaaccga catcatcgaa gcctcggccc gggcctatgt ccgcgcgctc ggcaacctgc
     7921 tggaaggcag cgccatccgc caggccgagg aagccacctc ggaagccgtc aaggaccacg
     7981 ccccctagtt cgtgccccgc ggtttcgccg cgggaccggt ggttgcgccg cggg
//
LOCUS       DAOQKN010000115         7977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_4408_length_7977_cov_4.879955, whole genome shotgun sequence.
ACCESSION   DAOQKN010000115 DAOQKN010000000
VERSION     DAOQKN010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 7977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7977
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4408_length_7977_cov_4.879955"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="PLJ64_08325"
     CDS             complement(<1..473)
                     /locus_tag="PLJ64_08325"
                     /inference="COORDINATES: protein motif:HMM:NF013492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNC93527.1"
                     /translation="MASEDETATLSDEAAAYAEGMERAEEALAEAGADGAAGEEVEVE
                     PEGGEEPVRHYDEETLRRREAALARIVQYGDPMLKSRSSEVTDFDQALTDEIERMFEL
                     MKDGLGVGLAAPQAGKLRRLLVFQTNADAEPQELVNPDIEWLSDETEVAAEGCLS"
     gene            complement(557..2746)
                     /gene="priA"
                     /locus_tag="PLJ64_08330"
     CDS             complement(557..2746)
                     /gene="priA"
                     /locus_tag="PLJ64_08330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNC93528.1"
                     /translation="MSIARVEPLTTARALRGPFDYLIPERLEGIGAGTVLEVPFGRRR
                     ITGVVVEVAETSEVPPGKLAEPIRVLGSGTNPDLIELGRWIASEYCSTPARGLGLVLP
                     PGTGTGGERTRFRVEKLNSITEAGREALDGGARLGSKQKLALQQLLEGDLNGRQLAAR
                     AGIDSATLKRLEERGLIERQQVHVRRRPDGAGVGSAATSAPDLTGAQAAAVARLVANL
                     DQPSAGEGVLLNGVTGSGKTEVYLAVVEEVLARGRTAIVLVPEIGLTPQAVGRFQARL
                     GDRVAVLHSALTAGQRFDEWQRLKAGDATVCVGPRSAVFAPLENLGLIVIDEEHDPSY
                     KQEGDPRYDAREVARKRALDTGSLLVLGTATPRPESWDRLVRIDLPDRVDGQAMPPVE
                     LVDMREADPRQGPIHRRTWEALAEVREKGEKAIVMINRRGFAPWLTCQTCGHHWGCPN
                     CDVSLIVHRESDQLICHHCNHAEPLPRRCPDCGGTTLSQTGAGTQRIERLLAEELAPM
                     PVFRLDADTSAGPGGHATILSAFDQEESAILVGTQMVAKGHDFPEVTLAAILDADATL
                     RFPDFRAEERTFSLVTQLAGRSGRSRTGGKVIVQTLAPGSPSISHAATHDSAGFLAGE
                     LDRRRELGYPPFSHLIRIQLAAEAEPEVERAANLVAARLDDALPAGAEMLGPAPMFRA
                     RNRYRRRLLVRSTTRAESIAAVHSTIEELLADKSLRKITLAIDVDPQ"
     gene            2834..3340
                     /locus_tag="PLJ64_08335"
     CDS             2834..3340
                     /locus_tag="PLJ64_08335"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HNC93529.1"
                     /translation="MAFQPSPGEQIIFQGHPSWRSILAFYLKGLLIGLIVAVIAKLLG
                     EGFATVFIIVLIALGLSVLIGFIKRMATVYTITNRRLNIKRGIFARDIQETRLERVQN
                     VSYNQTVFQRMLRVGDVDFDTAATDQDNLFCFHGVADPQEVVTEVDKATSLGASGSHG
                     LGEPSGQY"
     gene            3430..5472
                     /gene="tkt"
                     /locus_tag="PLJ64_08340"
     CDS             3430..5472
                     /gene="tkt"
                     /locus_tag="PLJ64_08340"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNC93530.1"
                     /translation="MTDSLASQSPELLAINTIRTLSIDAIDKANSGHPGTAMALAPLA
                     YKIWQDYLRYDPADPHWPNRDRFVLSAGHASMLIYSVLHLAGVKKVEHDGTISDRLSV
                     TLEDIENFRQLGSSTPGHPESHLTTGVETTTGPLGQGVATSVGMAIAGLWKRAAFGEE
                     LFDYDVYTICGDGDLMEGVSQEAASLAGHQQLHNLCWIYDSNRITIDGSTDLAFTEDV
                     EARFVANGWNVHHVNDANDLDEIGVALDSFEAEKEKPTFILIHSHIGYGSPNKVDTAD
                     VHGNPLGAEETKLTKEVYGWPVDAHFLVPDGVYDTWNNGVGKRGAELSAAWKDRFDGA
                     PEDTKNLIETMLSGGLPDGWDADIPAFEAGEQVATRSAAQKTLQAIAPKVPWLISGSA
                     DLTGSASSGLKEEVSGVFDPENRSGNMLHLGVREHESAASSNGMALSSLRPVWSTYLI
                     FSDYARPAIRLSALMEIPVVHWLTHDSIGLGEDGPTHQPVEQLASLRAMPNLDVIRPA
                     DAAEVAEAWKVIMPRTEHPTALVLTRQKVPVIDRSKFADASGLAKGGYVIADCDGEPE
                     LILIATGSEVHIALEAYETLTIEGVKVRVVSLPSWHIFENQDEAYRDSVLPPSVTRRI
                     SIEQASTFGWERYIGEEGVAIGMTTFGASAPFKDLQKYFGFTPDRVVEEAKNLLGS"
     gene            5477..7108
                     /gene="pgi"
                     /locus_tag="PLJ64_08345"
     CDS             5477..7108
                     /gene="pgi"
                     /locus_tag="PLJ64_08345"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF001211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNC93531.1"
                     /translation="MSELRESEAFKTLESHRNDIGDRHLRDLFAEDPSRGTDLVAEAC
                     GLYFDYSKNRITRETIGLLAALARDRRMPERRDAMFRGEHINVSEDRAVLHVALRMPR
                     SRSLVVDGVDVVREVHEVLDRMAAFSESVRSGEWKGQTGKPIRRIINIGIGGSDLGPV
                     VAYEALKDYSRRDLEFRFVSNVDGTDFYEATLDADPAETLFIVSSKTFTTLETMTNAH
                     TARQWLLDGLGGDESAIARHFVAVSTNAEGVSEFGIDTDNMFGFWEWVGGRYSMDSAI
                     GLSTMLAIGPDRFSEMLDGFHAMDEHFLETNLETNLPALHGLLTVWYRNFFDAETHAV
                     FPYSEYLHRFPAYLQQLTMESNGKGVTLDGTRVDYGTGAIFWGEPGTNGQHSFYQLVH
                     QGTELIPADFIGFNHALNPLGNHQDLLVSNVFAQTEALAFGKTVEQLQAEGVDERLIP
                     HKTFLGNRPTSTIMVDRLTPKSLGSLVALYEHSVFTQGAIWGIDSFDQWGVELGKALA
                     QAIIPELQSEEPPPLDHDSSTAELIRRYREANGRR"
     gene            7176..7889
                     /locus_tag="PLJ64_08350"
     CDS             7176..7889
                     /locus_tag="PLJ64_08350"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNC93532.1"
                     /translation="MTASVEAPIRAVIFDLDGTLLTTGGAGAVAWDKAFQEVFGKSVD
                     IAKVTESGMTDHDVAFAALRTVLDREPSHQDIDELTPVYVRELPEAVASSEKYRIEPG
                     IVELLERLASEGIPLGMTTGNIEPAARIKMERGDLNRFFAFGGYGSDSPNRAELTLHA
                     IKRGVVTCATGSFPNGRPQVLSKDDFIAVGDTPRDVSAAHEAGIRIVSVATGLFDLET
                     LKAAGPEWALPTVEEGFPV"
BASE COUNT         1342 a         2768 c         2519 g         1348 t
ORIGIN      
        1 atgctcaggc aaccttcggc cgcaacctcg gtctcgtcgg aaagccattc gatgtcgggg
       61 ttgacgagtt cctgcggctc cgcgtcggca ttggtctgga agacgagcaa ccggcgcagc
      121 ttgccggcct gcggtgccgc cagcccgacc ccgagaccgt ccttcatcaa ctcgaacatc
      181 cgctcgatct cgtcggtcag ggcctggtcg aagtcggtca cctccgagga gcggctcttc
      241 agcatcgggt cgccgtactg gacgattcgg gccagcgcag cctcgcgccg gcgaagcgtc
      301 tcttcgtcgt agtgacggac cggctcttcg ccgccctccg gctcgacttc cacttcttcc
      361 ccggcagccc catcggcgcc ggcctcggcc agcgcctctt cggcccgctc catgccctcg
      421 gcatatgcgg ccgcttcgtc gctcagggtg gcggtttcat cttcggaagc catcgtcgcc
      481 gagtctaccg acgcccggaa tcgcgcagga gccgtggaaa cccccgtcaa ggggcgagcc
      541 tgcctgctgc gcctgatcac tggggatcga cgtcgattgc cagcgtgatc ttcctcaggg
      601 atttgtcggc gagcagttcc tcgattgtgc tgtggactgc cgcgatcgat tcggctctgg
      661 tcgttgagcg gaccaggagg cggcggcggt agcggtttcg tgcccggaac atcggggccg
      721 ggccgagcat ttccgctccg gcggggaggg cgtcgtcgag gcgggcggca accaggttcg
      781 cggcccgctc cacttccggc tcggcttcgg ccgcgagctg gatgcggatc agatgggaaa
      841 acggcggata gcccagttcg cggcgtcgat cgagctcgcc ggccagaaag ccggcggaat
      901 catgggtcgc ggcgtggctt atcgaggggc tgcccggagc cagcgtctga acgatcacct
      961 tccccccggt ccggctgcgg ccgctgcggc cggccagctg ggtgaccagc gaaaaggttc
     1021 tctcctcggc ccggaagtcg gggaagcgga gggtggcatc ggcgtcgagg atcgcggcga
     1081 gggtcacttc ggggaagtcg tgccccttgg cgaccatctg ggtgccgacc agaatcgcac
     1141 tctcctcctg gtcgaaggca ctgaggatcg tcgcgtggcc acccggaccg gcgctggtgt
     1201 cggcgtcaag gcgaaagacc ggcatcggag ccagctcctc ggccagcagc cgttcgatcc
     1261 gctgggtccc ggccccggtc tgcgagagcg tcgtcccgcc gcagtcgggg cagcgccggg
     1321 gcagcggttc ggcgtgattg cagtggtggc agatcagctg gtccgactcc cgatggacga
     1381 tcagcgagac atcacagttc gggcagcccc aatggtgccc gcaggtctgg caggtaagcc
     1441 agggcgcaaa gccccggcgg ttgatcatca cgatcgcctt ctcgcccttc tccctcacct
     1501 cggccagggc ttcccaggtg cggcggtgga tcggaccctg ccgagggtcc gcctcccgca
     1561 tgtcgaccag ctcgaccggc ggcatcgcct ggccgtcgac ccggtccggc aggtcgatcc
     1621 ggaccaggcg atcccagctc tcgggccggg gggtggcggt gccgagcact agcagggaac
     1681 ccgtgtcgag cgcccgcttg cgggccacct cgcgggcgtc gtaacggggg tcgccttcct
     1741 gcttgtaaga gggatcgtgt tcctcgtcga tcacgatcag gccaaggttc tccagcggag
     1801 cgaagacggc cgaccgcggg ccgacgcaga cggtggcgtc gcccgccttc aggcgctgcc
     1861 actcgtcaaa gcgctggccg gcggtgaggg cggagtgaag gaccgcgacc cggtcaccga
     1921 gccgggcctg gaagcggcct acggcctgcg gggtcaggcc gatttccggc accagcacga
     1981 tcgcggtccg gccccgggcc agaacctcct cgaccacggc gaggtagacc tcggtcttgc
     2041 cggaaccggt caccccgttc agcaacactc cctccccggc ggaaggttga tcaaggtttg
     2101 cgaccagcct ggcgaccgcg gccgcctgtg ccccggtcag gtcgggggcg ctggtcgccg
     2161 cgctgccgac cccggccccg tcgggccgtc gccgcacgtg aacctgctgc ctctcaatca
     2221 ggccccgttc ctcaaggcgc ttgagggtcg cggaatctat gcccgcccgg gcggccagct
     2281 ggcggccgtt caggtcaccc tcgagcagtt gctgaagggc cagcttctgt ttggagccga
     2341 ggcgggcgcc accgtccagt gcttcccggc ccgcctcggt gatcgagttc agtttctcga
     2401 cccggaagcg ggtccgttca ccgccggtac cggtgccggg cggcagcacc aggccgagtc
     2461 cgcgggccgg ggtcgagcag tactcggagg cgatccagcg gccgagctcg atcagatccg
     2521 gattggtgcc ggatcccagg acccggatcg gctcggccag cttcccgggc ggcacctcgg
     2581 aggtctccgc cacctccacc accacaccgg tgatgcggcg ccggccgaaa ggcacttcca
     2641 gcaccgtgcc cgcgccgatc ccctccagcc tttccggaat caggtagtcg aaggggcccc
     2701 gcagagcgcg ggccgtcgtc agcggctcga ccctggcgat ggacaccggt cgaggttacg
     2761 cgggcgaacg gacgagcgac gccagaccgg ccggctttca cacatatccg ctccggtcga
     2821 atatgctcgc gccgtggctt ttcaacccag tcccggcgag cagattattt tccagggcca
     2881 cccgtcctgg cgttcgattc tcgccttcta cctgaagggc cttttgatcg gcctgatcgt
     2941 cgcggtcatc gcgaaattgc tcggcgaggg tttcgcaacg gtcttcatca tcgtcctgat
     3001 cgcgctcgga ctttccgtgc tgatcggttt catcaagcgg atggcgaccg tctacacgat
     3061 caccaaccgc cggttgaaca tcaagcgggg catcttcgcc cgcgacatcc aggagacccg
     3121 gctcgagcgg gtccagaacg tcagctacaa ccagaccgtg ttccagcgga tgctgagggt
     3181 cggcgacgtc gacttcgaca ccgccgcaac cgaccaggac aacctgttct gctttcacgg
     3241 ggtcgccgat ccgcaggaag tggtgaccga ggtggacaaa gcgacctcgc tcggcgccag
     3301 cggttcgcac gggctcggcg agcccagcgg ccagtactga gcgcagcgcc gacgcgcccc
     3361 cggcgcattc acgcaaaatc cgcatttcct gttctctggc ctgaagcggt tttccgtagg
     3421 attgcgggcg tgaccgactc cctcgcttcc cagtctcccg agctcctggc aatcaacacc
     3481 atccggacgc tttcgatcga tgcgatcgac aaggccaact caggccaccc cggcaccgcg
     3541 atggccctgg ccccgctggc ctacaagatc tggcaggact acctccgcta cgacccggcc
     3601 gatccgcact ggccgaaccg tgaccgcttc gtgctttcgg ccggtcacgc ttcgatgctg
     3661 atctactcgg tcctgcacct cgccggagtc aagaaggtcg agcacgacgg cacgatttcc
     3721 gatcgccttt cggtcaccct cgaggacatc gagaacttcc gccagctcgg ctcctccacc
     3781 cccggtcacc cggagtcgca cctgaccacc ggcgtcgaga cgaccaccgg cccgctcggc
     3841 cagggtgtcg ccacctcggt cggcatggcg atcgccgggc tctggaagcg tgccgcattc
     3901 ggtgaagagc ttttcgacta cgacgtctac acgatctgtg gtgacggcga tttgatggag
     3961 ggtgtctcgc aggaggccgc ctccctggcc ggtcaccagc agctccacaa cctctgctgg
     4021 atctatgact cgaaccggat cacgatcgac ggttcgaccg atctggcctt caccgaggac
     4081 gtcgaggcgc gcttcgtcgc caacggctgg aacgtccacc acgtaaatga cgccaatgac
     4141 ctcgacgaga tcggcgtcgc cctcgacagc ttcgaggccg agaaggaaaa gccgaccttc
     4201 atcctgatcc acagccacat cggttacggg tcgccgaaca aggtggacac cgccgacgtt
     4261 cacggcaacc cgctcggggc cgaggagacg aagctgacca aggaggtcta cggctggccg
     4321 gtcgatgccc acttcctggt tcccgatggt gtctacgaca cctggaacaa cggcgtcggc
     4381 aagcgcggag ccgagctttc ggccgcctgg aaggatcgct tcgacggcgc tcctgaagac
     4441 acgaagaacc tgatcgagac catgctttcc ggtggtctgc ccgacggctg ggacgccgac
     4501 atccccgcct ttgaagccgg cgagcaggtc gcgacccgct cggccgcgca gaagacgctg
     4561 caggcgatcg caccgaaggt gccgtggctg atcagtggct cggccgacct gaccggttca
     4621 gcctccagcg gcctgaagga agaggtctcc ggggtcttcg atcccgaaaa ccgctccggc
     4681 aacatgcttc acctcggggt ccgcgagcat gaatccgccg cctcctcgaa cggcatggcg
     4741 ctctcctcgc ttaggccggt ctggtcgacc tacctgatct tctccgacta cgcccgcccg
     4801 gcaatccgcc tctcggccct gatggagatc ccggtcgtcc actggctgac tcacgattcg
     4861 atcggcctcg gcgaggacgg cccgacccat cagccggtcg agcagctcgc ctcgctccgg
     4921 gcgatgccga acctggacgt gatccgcccg gccgacgcgg ccgaggtcgc cgaagcctgg
     4981 aaggtgatca tgccgcgcac cgaacatccc accgccctgg tcctgacccg ccagaaggtg
     5041 ccggtgatcg accgcagcaa gttcgccgac gcttccgggc tggcgaaggg cgggtacgtg
     5101 atcgccgact gtgacggcga gccggagctg atcctgatcg cgactggctc cgaggtccac
     5161 atcgcactcg aggcatacga aaccctgacc atcgagggcg tcaaggtccg ggtcgtttcg
     5221 ctgccttcct ggcacatctt cgaaaaccag gacgaggcgt accgggattc ggtccttccg
     5281 ccttcggtca cccggcggat ctcgatcgaa caggcttcga ccttcggctg ggagcgctac
     5341 atcggcgagg aaggcgtggc gatcggcatg accaccttcg gcgcgtcggc cccgttcaag
     5401 gacctccaga aatacttcgg cttcaccccg gaccgggtgg tcgaagaagc gaagaacctg
     5461 ctcgggagct gaaccggtga gcgagctccg ggagtcagaa gccttcaaga ccctcgagag
     5521 ccaccgcaac gacataggcg accgtcacct gagggatctc ttcgccgagg atccctcccg
     5581 cgggaccgat ctggtcgccg aggcctgcgg tctctacttc gactactcga agaaccggat
     5641 cacccgtgag acgatcggcc tgctcgctgc cctcgcccgg gaccgccgca tgccggagcg
     5701 tcgcgacgcg atgttccggg gcgagcacat caacgtctcc gaggaccggg cggtgctgca
     5761 tgtggcgctg cggatgccgc gcagccgctc gctggtcgtc gacggggtcg acgtggtgcg
     5821 ggaagtccac gaggtcctcg accgcatggc cgccttctcc gagtcggttc gttcgggcga
     5881 atggaagggc cagaccggca aaccgatccg gcggatcatc aacatcggca tcggcggatc
     5941 tgacctcggc ccggtggtgg catacgaggc gctcaaggac tattcccgcc gtgacctcga
     6001 gttccgtttc gtttctaacg tggacggcac tgacttctac gaggcgaccc ttgacgccga
     6061 cccggccgag acgctcttca tcgtttcctc gaaaaccttc acgaccttgg agacgatgac
     6121 caacgcccac accgcccgcc agtggctgct cgacggcctc ggcggtgacg agtcggccat
     6181 tgccaggcac ttcgtcgcgg tctcgaccaa cgccgaagga gtctcggagt tcgggatcga
     6241 caccgacaac atgttcggat tctgggaatg ggtcgggggc cgctactcga tggattcggc
     6301 gatcggcctc tcgaccatgc tggcgatcgg ccccgatcgt ttcagcgaga tgctcgacgg
     6361 tttccacgcg atggacgagc acttcctcga aacaaatctc gagaccaacc tgccggccct
     6421 ccacggcctg ctcacggtct ggtaccggaa cttcttcgat gccgagaccc acgccgtctt
     6481 cccctacagc gagtatctgc accgcttccc cgcctacctc cagcagctga ccatggagtc
     6541 gaacggcaag ggcgtgactc tcgacgggac ccgggtcgat tacggcaccg gcgcgatctt
     6601 ctggggtgag ccggggacca acggccagca ttccttttac cagctggtcc accagggaac
     6661 cgagctgatc ccggccgact tcatcggttt caaccatgcc ctcaacccgc tcggcaacca
     6721 tcaggacctg ctggtctcca acgtcttcgc ccagaccgag gcgttggcct tcggcaagac
     6781 cgtcgagcag cttcaggccg aaggcgtcga cgagcgcctg atcccccaca agaccttcct
     6841 cggcaaccgg ccgacctcga cgatcatggt tgaccgcctg accccgaagt cgctcggttc
     6901 cctggtcgcc ctctacgagc attccgtctt cacccagggt gcgatctggg gcatcgactc
     6961 cttcgaccag tggggggtcg aactgggcaa ggccctggcc caggcgatca tccccgaact
     7021 tcagtccgaa gagccaccgc cgcttgatca cgacagttcg accgcggagc tgatccgtcg
     7081 ctaccgcgag gcaaacggcc gccgatgaaa tcgagaaggc aaaagtggcc atatagcgct
     7141 ccattcagcc ttctcgttgc cggggacggc tgcccatgac cgcctcggtc gaggccccga
     7201 tccgcgcggt cattttcgac ctcgacggca ccctgctgac caccggcggg gccggcgcgg
     7261 tcgcctggga caaggccttt caggaagtgt tcggcaagtc ggtcgacatc gccaaggtga
     7321 ccgagtcggg catgaccgac cacgatgtcg ccttcgctgc ccttcgcacc gtgctcgacc
     7381 gcgagccaag ccatcaggac atcgacgaac tgaccccggt ctatgtccgc gagctcccgg
     7441 aggcggtcgc cagttcggag aagtaccgga tcgaaccggg aatcgtcgaa ctgctggaaa
     7501 ggcttgcgtc cgagggaatc ccgctcggca tgaccaccgg aaacatcgag ccggcagcca
     7561 ggatcaagat ggagcgaggt gacctcaacc gcttcttcgc cttcggcggc tacgggtccg
     7621 attcacccaa ccgggccgaa ctcaccctcc acgcgatcaa gcgcggcgtg gtgacctgcg
     7681 ccaccggcag cttccccaac ggccggcccc aggtcctctc caaggacgat ttcatcgcgg
     7741 tcggcgacac cccccgcgac gtctccgccg cccacgaagc cggcatccgc atagtcagcg
     7801 tcgccaccgg cctcttcgac ctggaaacac tgaaagcagc aggacccgag tgggccctgc
     7861 cgacggtcga agaaggcttc cctgtctgag tgcaattggg gcgggttcga cccgctcaag
     7921 atcgagatgg cagaagtcag cggatatgac tgacttctgc cttctcgatt cgaaacg
//
LOCUS       DAOQKN010000116         7929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_4447_length_7929_cov_5.904115, whole genome shotgun sequence.
ACCESSION   DAOQKN010000116 DAOQKN010000000
VERSION     DAOQKN010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 7929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7929
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4447_length_7929_cov_5.904115"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="PLJ64_08355"
     CDS             <1..210
                     /locus_tag="PLJ64_08355"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936107.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNC93533.1"
                     /translation="TLNTIKMLDIDPEKMNVNGGAVALGHPIGASGARVIGALVRELQ
                     RRGGGLGCAAICSGGGQGDAILVEV"
     gene            176..1549
                     /locus_tag="PLJ64_08360"
     CDS             176..1549
                     /locus_tag="PLJ64_08360"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HNC93534.1"
                     /translation="MVRVTPSSSKSEVERKFLVPEVPDGLSGPSGVSIEQGYLAIDDD
                     SEVRLRRAGEALTLTVKSGHGESREETEVEVDQATFDDLWPESEGRRVEKLRRRIDLD
                     QGLVAELDIYSGDLDGTSVVEVEFESEAAARAFQPPAWFGRELTGDREWANQSLAVRG
                     RPSKRDEFRLRPGEGPATGVCRVIAARAAQAAAAVRQAGEAEDSATPVHEARKSLKKV
                     RSGLRLLRSVIPDDERTKVNETCRGAASRLSGARDAEVKIATLNSVTGPNPSPDGAGD
                     WLGELEEEAEGHRGELNPGSLAEVHDAIDGVRRTFLGRDPAAGPETVAGNAGRGYRRG
                     RKAMKRAKESGEAEDFHDWRKRSKDLRYQLEILGPRLPEEFDQLRKEATDLADRLGDL
                     HDLDVLAGDLESRDLEPEDKPALAELIGRARDRQVEACLDLGAVVYEMKPGRLTDLIR
                     EALSDEA"
     gene            complement(1533..2726)
                     /locus_tag="PLJ64_08365"
     CDS             complement(1533..2726)
                     /locus_tag="PLJ64_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93535.1"
                     /translation="MAENEGERPREMRKTLIVMLLWLVAGITPALASPPPVSDWVQTN
                     RVQFSGTDGFFRSQGVASDGQSLFFSWNLGLSRTAINDTSNVLADNTTEAIPDDLAES
                     NHNHIGDIDVAWGWIYAPIEDGPAYAEPWVVIYDADTLEPTGQRFLLPASLQRDGVPW
                     IAVDQVRGVAYSMEWKDTGKLFVYSLSDFSLLRTVTLSEPVPRIQGAKIYRGNLYASR
                     DNGSEKSIVAIDPETGTVTHLFDRNLGEDYEAEGIAFVRRSSGTMMVATGIREGSQGY
                     TEMRIYRIGGDTTAPALSGVSFKAKRVRPGSKVTLLATSSEPVTALVRWLRCIGPAAR
                     PCSRTRVFGSNDRIQITEGSNQVSLSTALLTPGIWNLRITPSDRADIVGRTRRVGLTV
                     LRPRR"
     gene            2744..3514
                     /locus_tag="PLJ64_08370"
     CDS             2744..3514
                     /locus_tag="PLJ64_08370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HNC93536.1"
                     /translation="MVQAAFFDLDKTLMAGSSGMQFAKAAQERGLISRAQILRWGRDH
                     IRYRIKGATDDETGEILKVARETLSGVSYNEISRMWPEVLAGILPRIYPEVLAEVHRH
                     QDEGRLTYIVSAAGSDMVESLAQVLGMDGGIGTRYVVEDGIYTGALDGPFVYGRGKVK
                     AIRWLSELREQTGEEPFDLEGSWAYSDSASDLPMMELVGNAVAVNPDAPLLEKARREG
                     WTVMRFDRLGRNLAIGGTVAGALLVGGIGGWLSKRRAG"
     gene            3600..5237
                     /locus_tag="PLJ64_08375"
     CDS             3600..5237
                     /locus_tag="PLJ64_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNC93537.1"
                     /translation="MEPTSTSSSSDAARKAAEAADNEFTTMSGKPIKARYGPEDLSGD
                     ADVKIGEPGQYPFTRGPYESMYRGRNWTMRQFAGFGTVEETNERFHYLLDHGQTGLST
                     AFDMPTLMGYDSDHARSLGEVGREGVAVDTIDDMEILFQGIPLGEVSTSMTINAPAAI
                     LLAFYVLVGEQQGVASEKLSGTIQTDILKEYIAQKEWCFPVEPAMRLVTDMVEYCTDH
                     MPRWHPISISGYHIREAGATAAQELAFTLKDGFTYVERAIERGIDVDAFAPRLSFFFN
                     AHIDFFEEIAKYRAARRIWARELKETYGAKKPESMRLRFHTQTAGVSLTAQQPLNNIV
                     RTAIEALAGVLGGTQSLHTNSFDEALALPTEEAVRVALRTQQIIADETGVTNTPDPLG
                     GSYFVESLTDELEADAYDYFRRIDELGGMVEAVRQNFPQREIGEAAFDFQRRVDDGEF
                     TIVGVNQYVTEEEEVPLLHIDPALETKQVERLAATRAKRDSAEVESALTALKDAAAGE
                     ANLMYPILDAARVHTTEGEMIEAMQQVFGTYTETPVF"
     gene            5261..5692
                     /locus_tag="PLJ64_08380"
     CDS             5261..5692
                     /locus_tag="PLJ64_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HNC93538.1"
                     /translation="MSAATAEPKTDRKIRVVVAKPGLDGHDRGAKIIARALRDSGMEV
                     IYTGLHQTPEQIVETVIQEDADAIGVSILSGAHMTLVPKILELLEAEGIDDVLITVGG
                     TIPNKDIDALKEMGVSGVFTPGAPTDDIVNFIRTGVSRREL"
     gene            5689..6792
                     /locus_tag="PLJ64_08385"
     CDS             5689..6792
                     /locus_tag="PLJ64_08385"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HNC93539.1"
                     /translation="MIETFTLRDVGPKDGLEQAEMVAGRITDFIGKAEQSLVLALYDI
                     RLPDPVGETVAQAFRDAAARGVKVRLAYNDVPEDPDAGNFLPAVNPPPETNPEILARL
                     PIETKPIKGIPDLMHHKYMVRDEKAVWTGSSNWSVYSWTRQENVLGIIDHGEVAREYL
                     TDFEELWQKHKVERSGFGDPNPIRVGEATVRVWFTPGHGKALAQRISSKLGSARRRIR
                     IASPVLTSGPILSTLAEIANRPGLDIAGVLDEPQTDRVYEQWATTGSKWKIPLLTQVV
                     ETIPFSGKRSVPYGTGTVRDFMHAKVTVADDTVFFGSFNLSRSGEANAENMVEIEDAG
                     IAEKMAKFIDETRALYPRSTVPDRRGPRKGERT"
     gene            6792..7772
                     /locus_tag="PLJ64_08390"
     CDS             6792..7772
                     /locus_tag="PLJ64_08390"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC93540.1"
                     /translation="MLPLRGKAAEALDGAMDFMVVPGFSRIGYEVRRRMFDWQMPDLA
                     GRSVMVTGATSGLGEAAAIDLARCGAIVHIVARNPDKAEETRAEIQSAAPGAPVSVHL
                     CDLSLTTDIRRFTDAFLESGDRLDVLINNAGVMPAEREYTDEGFELTFATNLIGPYLL
                     TELLLPRLREGDNPRVITMSSGGMYSSGFSFRDPQLAEKDYKPTSFYAHTKRGEVMIT
                     DAWQRREPEGGVTFCSMHPGWAVTPGMSAALPGFHKVTGPILRDSHEGVDTAIWLAGA
                     TPAEAPGGTFYEDRRPRTKQRMPGTAGSSEDGERLLELLAGITSGSDAEG"
BASE COUNT         1428 a         2609 c         2637 g         1255 t
ORIGIN      
        1 accctgaaca cgatcaagat gcttgacatc gaccccgaaa agatgaacgt caacggcggc
       61 gccgtcgccc tcggccatcc gatcggcgct tccggcgccc gggtgatcgg cgccctcgtt
      121 cgcgagctgc agcgccgcgg cggaggcctt ggctgcgccg cgatctgctc cggcggtggt
      181 cagggtgacg ccatcctcgt cgaagtctga ggtagagcgg aagttcctcg ttcctgaagt
      241 gccggacggg ctttcgggcc cgtccggagt ttcgatcgag cagggctacc tggcgatcga
      301 cgatgattcc gaagtccggt tgaggcgggc cggcgaggcg ctgaccctga ccgtcaagag
      361 cggtcacggc gagtcgcggg aggaaaccga ggtcgaggtg gaccaggcca ccttcgacga
      421 cctctggccg gaatcggaag gcagacgggt cgagaagctc cggcgccgga tcgacctcga
      481 ccagggcctg gtcgccgagc tggacatcta ctcgggtgat ctcgacggga cctcggtggt
      541 cgaagtcgag ttcgaatcgg aagcggccgc acgggccttt caaccgcccg cctggtttgg
      601 ccgggagttg accggtgacc gtgagtgggc aaaccagtcg ctggcagttc gcggccggcc
      661 ctcgaagcgg gacgagttca gattgcggcc gggggaaggt ccagcgaccg gggtctgccg
      721 ggtgatcgcg gcccgggccg cccaggccgc ggcggcggtg cgccaggcgg gcgaggccga
      781 ggattccgcg acgcccgtcc acgaggcacg aaagagcctg aagaaagtcc gttccggcct
      841 gcgactgctg cgaagcgtga tccccgatga cgagcggacg aaggtcaacg agacctgccg
      901 cggagcagcc agccggcttt ccggtgcccg cgacgccgag gtcaagattg ccacgctgaa
      961 ttcggtgacc ggcccgaacc cgtcgccgga cggagccggg gactggctag gcgaactcga
     1021 agaagaagcg gaaggccacc gcggcgagct caacccggga agcctggctg aagttcatga
     1081 cgcgatcgac ggcgtccggc gcaccttcct tggccgggat ccggcggctg gcccggagac
     1141 ggtcgccggg aacgccggtc gtggttaccg gcgcgggcga aaggcgatga agcgggcgaa
     1201 agagagcggc gaggcggaag acttccacga ctggcgaaaa cggtcgaagg acctgaggta
     1261 tcaactcgag atactcgggc cccggttgcc ggaagagttt gaccagttgc gaaaagaagc
     1321 aacggacctg gccgaccggc tcggtgacct gcatgacctc gacgtgctcg ccggggatct
     1381 ggagagccgg gacctggaac cggaagacaa gcctgcgctg gccgaactga tcggtcgcgc
     1441 ccgggaccgg caggtcgaag cctgcctcga cctcggtgcc gtggtctacg aaatgaagcc
     1501 cggccggctc accgacctga tccgggaagc gctcagcgac gaggcctgag cacggtcagc
     1561 ccgaccctcc gcgtccggcc gacgatgtcc gcccggtcac tgggggtgat ccggaggttc
     1621 cagatgccgg gcgtgagcag cgcggtcgag agcgacacct ggttgctccc ctcggtgatc
     1681 tgaatccggt cgttcgaacc gaagacccgg gtcctcgagc agggcctggc cgccggaccg
     1741 atgcacctga gccacctgac cagcgcggtg accggctcgg aagaggtggc gagcagggtc
     1801 actttcgagc ccggtctgac cctttttgcc ttgaagctga ccccgctcag tgccggggcg
     1861 gtcgtgtcgc cgccgatccg gtagatccgc atctcggtgt agccctgtga gccttcacgg
     1921 atgccggtgg ccaccatcat cgtgccggag gaccggcgga cgaaggcgat gccctcggcc
     1981 tcgtagtctt cgccaaggtt gcggtcgaag aggtgcgtga cggtcccggt ctccgggtcg
     2041 atcgcgacaa tcgacttctc gctgccgttg tcacgcgatg cgtagaggtt gccgcggtag
     2101 atcttcgctc cctggatgcg gggcaccggc tcgctcaggg tcacggtgcg gagcaggctg
     2161 aagtcactca ggctgtagac gaagagcttg ccggtgtctt tccactccat cgagtaggcg
     2221 acgccacgga cctggtccac ggcgatccag gggacgccgt cacgctggag cgaggcgggc
     2281 aacaggaaac gctggccggt cggttcaagg gtgtcggcgt cgtagatcac cacccagggt
     2341 tcggcgtagg cggggccatc ctcgatcggg gcgtagatcc agccccaggc cacgtcgatg
     2401 tcgccgatgt ggttgtggtt gctttcggcc agatcgtcgg ggatcgcctc ggtggtgttg
     2461 tcggccagca cgttcgaggt gtcgttgatc gcggttcggc tgaggccgag gttccaggag
     2521 aagaagagcg actgcccgtc gctggccacg ccctgcgagc ggaagaagcc gtcggtgccg
     2581 ctgaactgga cccggttggt ctgaacccag tcggaaaccg ggggcgggct ggccagggca
     2641 ggcgttatgc cggccacgag ccacaacagc atcacgatca gcgtctttct catctcccgc
     2701 ggcctttctc cctcgttttc agccatcgct aacgtatccg ttcatggtcc aggcagcatt
     2761 tttcgacctc gacaagaccc tgatggcggg ctcctccggc atgcagttcg ccaaggcggc
     2821 tcaggagaga gggctgattt cgagggccca gatcctgcgc tggggccggg atcacatccg
     2881 ttaccggatc aagggcgcca ccgacgacga aaccggcgag atcctcaagg tcgcccggga
     2941 aacgctttcc ggggtttcct acaacgagat ttcccggatg tggccggaag tgctggccgg
     3001 catcctgccc cggatctacc ccgaggtact ggccgaggtt caccggcatc aggacgaagg
     3061 cagactcacc tacatcgtca gcgcggcagg atcggacatg gtcgagtcgc tggcccaggt
     3121 gctgggcatg gacggcggca tcggcacccg atacgtggtc gaggacggca tctacaccgg
     3181 ggcgctcgac ggtcccttcg tctacggccg gggcaaggtc aaggcgatcc gctggctttc
     3241 ggagctccgc gaacagaccg gcgaggaacc gttcgacctc gaaggctcct gggcctactc
     3301 cgactcggcc tccgacctgc cgatgatgga gctggtcggc aacgcggtcg cggtcaaccc
     3361 ggatgccccg ctgctcgaga aggcccgccg ggaaggctgg acggtgatgc gcttcgatcg
     3421 actcggccgc aacctcgcga taggcggcac cgtcgccggc gccctcctag tcggcgggat
     3481 cggcggctgg ctttcgaaaa gacgggcggg ctgaggaacc cgcctctcgt ggatatccac
     3541 tagtggatcg aggggctccg ctgccggtgg tccgaactgc cttaacatgg acctcgccta
     3601 tggagccgac ttccacatcc agttcttcgg acgctgcccg caaggcggcc gaggccgctg
     3661 acaacgagtt cacgaccatg tcgggcaagc cgatcaaggc ccggtacggt cccgaggatc
     3721 tgagcggcga cgccgacgtc aagatcggcg agccgggcca gtaccccttc acccggggtc
     3781 cttacgaatc gatgtaccgg ggtcgcaact ggacgatgcg ccagttcgcc ggctttggca
     3841 ccgtcgagga aaccaacgag cgcttccatt acctgctcga tcacggccag accgggcttt
     3901 cgaccgcctt cgacatgccg accctgatgg gctacgactc cgaccacgcg cgatcgctgg
     3961 gcgaggtcgg ccgcgaaggc gtcgccgtcg acacgatcga tgacatggag atcctcttcc
     4021 agggcatccc gctcggcgag gtctcgacct cgatgacgat caacgccccg gcggcgatcc
     4081 tgctcgcctt ctacgtgctg gtcggcgagc agcagggcgt cgcctcggag aagctctcgg
     4141 gcacgatcca gaccgacatc ctcaaggagt acatcgccca gaaggagtgg tgcttcccgg
     4201 tcgagccggc gatgcgcctg gtcaccgaca tggtcgagta ctgcaccgac cacatgccgc
     4261 gctggcatcc gatctcgatc tccgggtacc acatccgcga ggccggggct accgccgcgc
     4321 aggaactcgc cttcaccctc aaggacgggt tcacctacgt cgaacgggcg atcgagcgcg
     4381 gcatcgacgt cgacgccttc gcgcccagac tttcgttctt cttcaacgcc cacatcgact
     4441 tcttcgagga aatcgccaag taccgcgccg cccgccggat ctgggcccgt gagctcaagg
     4501 agacctacgg agcgaagaag cccgagtcga tgcggcttcg cttccacacg cagaccgccg
     4561 gggtctcgct gaccgcacag cagccgctga acaacatcgt ccggaccgcg atcgaggccc
     4621 tggccggggt gctcggcggc acccagtcgc ttcacaccaa ctccttcgac gaggccctcg
     4681 ccctgccgac cgaggaagcg gtccgggtcg cgctgcggac ccagcagatc atcgccgacg
     4741 aaaccggagt caccaacacg cccgatccgc tcggcggttc gtacttcgtc gagtcgctga
     4801 ccgacgagct ggaagccgac gcctacgact acttccgccg gatcgacgaa ctgggcggca
     4861 tggtcgaagc ggtccgccag aacttccccc agcgcgagat cggtgaagcg gccttcgact
     4921 tccagcgccg ggtggacgac ggcgagttca cgatcgtcgg ggtcaatcag tacgtgaccg
     4981 aggaagagga ggttcccctc cttcacatcg atcccgccct cgagaccaag caggtcgagc
     5041 gtctcgccgc gaccagggca aagcgcgact cggctgaagt cgagtccgcc ctgacggccc
     5101 tcaaggatgc cgcggcaggc gaggccaacc tgatgtaccc gatcctcgat gccgcccggg
     5161 tccacacgac cgaaggcgag atgatcgagg cgatgcagca ggtattcggc acctacacgg
     5221 agactccggt cttctagatc cgaaagcagg ttttcagctg atgagcgccg ccactgccga
     5281 accgaagacc gaccgcaaga tccgcgtggt cgtggccaag cccggcctcg acggccacga
     5341 ccggggagcc aagatcatcg cccgcgccct gcgcgactcc ggcatggagg tcatctacac
     5401 cggccttcac cagaccccgg agcagatcgt ggaaacggtg attcaggagg acgccgacgc
     5461 gatcggtgtc tcgatcctct ccggtgccca catgaccctg gtcccgaaga tcctcgaact
     5521 gctcgaagcc gaaggcatcg acgacgtcct gatcacggtc ggcggcacga tcccgaacaa
     5581 ggacatcgac gcgctcaagg agatgggcgt ctcgggagtc ttcactcccg gcgccccgac
     5641 cgacgacatc gtcaacttca tccgcaccgg cgtcagccgc cgcgagctgt gatcgaaacc
     5701 ttcaccctcc gtgacgttgg tccgaaggac gggctggagc aggcggagat ggtggccggg
     5761 aggatcaccg acttcatcgg caaggccgag cagagcctgg tcctcgccct ttatgacatc
     5821 cggctgccgg acccggtcgg ggagaccgtg gcacaggcct tccgggatgc cgcggcccgg
     5881 ggagtcaagg tgcgactcgc ctacaacgac gtgccggaag atcccgatgc cggcaatttc
     5941 ctgccggccg tgaacccgcc gccggagacc aatccggaga tcctggccag actgccgatc
     6001 gaaacgaagc cgatcaaggg catccccgac ctgatgcacc acaagtacat ggtgcgggac
     6061 gagaaggcgg tctggaccgg ctcctcaaac tggtcggtct actcctggac ccggcaggag
     6121 aacgtgttgg ggatcatcga tcacggcgag gtcgcccgcg aatacctgac cgacttcgag
     6181 gaactctggc aaaagcacaa ggtcgagcga tccggctttg gtgatcccaa tccgatcagg
     6241 gtgggggagg ccacggtccg ggtctggttc acccccggtc acggcaaggc gcttgcccaa
     6301 cggatctcgt cgaagctcgg ttcggcccgc cgccggatcc ggatcgcctc gccggtcctg
     6361 acttccggcc cgatcctcag caccctggcc gagatcgcga accggcccgg actggacatc
     6421 gccggggtgc tcgacgaacc acagacggac cgcgtctatg aacagtgggc gaccaccggc
     6481 tctaagtgga agattccgct gctcacccag gtggtggaaa cgattccgtt cagcggcaag
     6541 cggtcggttc cctacggcac cggcaccgtc cgcgacttca tgcacgccaa ggtcaccgtc
     6601 gccgacgaca ccgttttctt cggttccttc aacctttccc gctcggggga agccaacgcc
     6661 gagaacatgg tggaaatcga ggatgcgggt atcgccgaga agatggcgaa attcatcgac
     6721 gagaccaggg cgctctaccc gcgcagcacc gtgccggacc ggcgcggccc gcgcaagggc
     6781 gagcgaacct gatgctcccg ctccggggca aggccgccga ggccctcgac ggagccatgg
     6841 acttcatggt ggtgccgggc ttcagccgga tcggctacga agtccgccgt cgcatgttcg
     6901 actggcagat gccggacctg gccggccgca gcgtgatggt taccggggca acctccggcc
     6961 tcggtgaagc cgccgcgatc gacctcgccc gttgcggggc gatcgtccac atcgtggctc
     7021 gcaatccgga caaggccgaa gaaacccggg cggaaatcca gtccgccgct cccggggccc
     7081 cggtcagcgt tcatctctgc gatctctcgc tgaccacgga cattcggcgt ttcaccgacg
     7141 cgttcctcga atcaggcgac cggcttgacg tgctgatcaa caatgccggg gtgatgccgg
     7201 cggagcgtga atacaccgat gagggattcg agctgacttt cgccaccaac ctgattggtc
     7261 cgtacctgct caccgagctt ctgctgcccc ggctgaggga aggcgacaac ccgcgggtga
     7321 tcacgatgag ttccggcggc atgtactcga gcgggttcag ctttcgcgac ccgcaactgg
     7381 cggagaagga ctacaagcca accagcttct atgcccacac caaacggggc gaggtcatga
     7441 tcaccgacgc ctggcagcga cgcgaacccg aaggcggggt gaccttctgc tcgatgcatc
     7501 cgggctgggc ggtcacaccg ggcatgagcg ccgcactgcc cggcttccac aaggtgaccg
     7561 gtccgatcct gcgggattcg catgaaggtg tcgacacggc aatctggctc gccggcgcga
     7621 ctccggcaga ggcgccgggc ggaaccttct acgaggatcg ccggccccgg accaagcagc
     7681 gaatgccggg gactgccggc tcctccgaag atggcgaacg gctgcttgag ctactggctg
     7741 gtataaccag tggctccgat gccgaggggt gatcattgaa accgggccgg ttcccgcccg
     7801 cgcagctgtc gattttgtgg ctggcggccc tggggctgtc cttcttcgcc ctgccgctgc
     7861 ccggccattc agcgaccgcc agggcggcgg gggaatgcac gctcatcccg ggcggcacga
     7921 tcggcgacc
//
LOCUS       DAOQKN010000117         7883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_4491_length_7883_cov_5.333674, whole genome shotgun sequence.
ACCESSION   DAOQKN010000117 DAOQKN010000000
VERSION     DAOQKN010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 7883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7883
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4491_length_7883_cov_5.333674"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /gene="dprA"
                     /locus_tag="PLJ64_08395"
     CDS             complement(<1..1025)
                     /gene="dprA"
                     /locus_tag="PLJ64_08395"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNC93541.1"
                     /translation="MSGRRRLGGAGVDARSACPECLRRSWLIARLGAWIQRVVDDRAG
                     SRTPELLRLDSETLVAAVAAKQADKILAWNASLTEGEMVAAIENAGCWACCRHHESFP
                     AGLNDGADSPVALIGRGGGLRLDEIELGNSVTVVGARKATGYGLEVAASIGREVAAAG
                     LNVISGLALGIDGAVHRGALERGPTVAVLGCGADRPYPASHSRLYRQVVESGLVISEV
                     PPGADAWRWSFPARNRIMAALSAMTVVVEAAWRSGSLITAEMASDAGRDVGAVPGPVT
                     AGAAAGTNELLANGAALIRGGQDVLDRMLGAGAGQPLFGPAIEDFEVELLEAVEAGCR
                     TVDEAAL"
     gene            complement(1022..2512)
                     /locus_tag="PLJ64_08400"
     CDS             complement(1022..2512)
                     /locus_tag="PLJ64_08400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNC93542.1"
                     /translation="MLAETTSFTLDGIAARMIRVEVDVHRGLPNFQIVGLPDAAVREA
                     RERVRAATVNCGFDFPLQRITASLAPADLRKAGPAMDLALATALLGAAGDLKIEPLAG
                     WSLVGELALDGSVRPVRGALAMAEEAREQGRRGVIVPTANGPEAALAEGITVLTIDNF
                     GELASLQRGEIPGRLAIPAVPGGSDEMPDLADLRGQGHLRAAVEISAAGGHGLLMIGP
                     PGAGKSLAARRLPSVLPPLAGEEVLEVARIASACGIGDGRISSTRPFRAPHHTVSAAG
                     LVGGGSPPRPGEITLAHRGVLFLDELCEFNRPALEALREPLESGSVTIARARGRQTLP
                     CRFTLVAAANPCPCGRGEEDPRCRCSEMSARRYRAALSGALVDRIDLVVAVTQPSAEA
                     LAGPPGEESPEVRKRVILARELMAERLGDGRTNSEAEPDEVANFKVTAAAAGLLAEAH
                     RSWQLSGRAHGRILRVARTVADLAGSTAIGEQHMATAIQFRRRSAQ"
     gene            2676..3050
                     /locus_tag="PLJ64_08405"
     CDS             2676..3050
                     /locus_tag="PLJ64_08405"
                     /inference="COORDINATES: protein motif:HMM:NF009154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNC93543.1"
                     /translation="MDDRRATGRSAEDICARRLRERGWRILARNWRIRPGELDLIALA
                     GSTLVIVEVKAHHQGLRRGPVHPVLAVGPNKQRRIRRLASAWIAGHGRSIGFREVRFD
                     VVGITFDREGRLAAYDHLEDAF"
     gene            complement(3088..3750)
                     /locus_tag="PLJ64_08410"
     CDS             complement(3088..3750)
                     /locus_tag="PLJ64_08410"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNC93544.1"
                     /translation="MGSSTARTPAAEQAPGELFSHDLELGASAVAGADEAGRGCLAGP
                     IVAAAVLFDRECLESQGGGRLAEIRDSKKLTAKARERLYPEVLDCAVKTVVVMRSARY
                     IDANGLHVSNMECLARAIEGLGSGPEAVTLVDGFALKGCEVPHRRLVKGDSTSAAIAA
                     ASILAKVTRDRCMARAGLAYDGYGFEAHKGYASPAHRDAIRLLGPSPIHRLSFASEAY
                     QA"
     gene            complement(3740..4321)
                     /gene="lepB"
                     /locus_tag="PLJ64_08415"
     CDS             complement(3740..4321)
                     /gene="lepB"
                     /locus_tag="PLJ64_08415"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNC93545.1"
                     /translation="MVVIVALALGLALLIQAFLIKPYQIPSGSMEPTLDINQRILVNR
                     FVYHFTDPKPGDVVVFHPPAGVDRGGLEECGAPHQQDQPCPQPTSDKSSETFIKRIVA
                     GPGDTVSIEKGLPVVNGEVVEPDGYEINPCGPAGTGCNMPKEITVPEGHFFMMGDNRG
                     HSDDSRFWGPLPEEWLIGKAFATYWPPNRIGGL"
     gene            complement(4738..5079)
                     /gene="rplS"
                     /locus_tag="PLJ64_08420"
                     /pseudo
     CDS             complement(4738..5079)
                     /gene="rplS"
                     /locus_tag="PLJ64_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
     gene            complement(5148..5807)
                     /gene="trmD"
                     /locus_tag="PLJ64_08425"
     CDS             complement(5148..5807)
                     /gene="trmD"
                     /locus_tag="PLJ64_08425"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNC93546.1"
                     /translation="MNLDIFTLFPEAFDWFRQQRPVRKAIEHGAELNLRDYRDWTPLK
                     GGRVDDAPYGGGAGMVLRVDVVDAALRGVYGDGERPRTVVLSPAGRVLDDGLVTELAA
                     EPGLALLCGRYEGFDHRVHEHLADDEISIGRYVLAGGELPAMVLADTIMRRQPGALGD
                     EESAIEESWSEALEGLPEYPHYTRPPEYRGWGIPDVLISGDHAKVREWRLEQSRKRGA
                     E"
     gene            complement(5851..6081)
                     /locus_tag="PLJ64_08430"
     CDS             complement(5851..6081)
                     /locus_tag="PLJ64_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HNC93547.1"
                     /translation="MQEMLLYITRALVEDPDAVEVEELEEDGDLVFEITVGEDDLGRV
                     IGRGGRVANAIRTIAKAAAVREERRVLVDILD"
     gene            complement(6096..6350)
                     /gene="rpsP"
                     /locus_tag="PLJ64_08435"
     CDS             complement(6096..6350)
                     /gene="rpsP"
                     /locus_tag="PLJ64_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNC93548.1"
                     /translation="MAVRIRLRRVGSKKNPIWRIVVADKRAPRDGRTIETIGQYNAQT
                     DPSTIVIDEERANYWLEKGAQPSERVAGLLRIKGIKATGS"
     gene            complement(6407..7771)
                     /gene="ffh"
                     /locus_tag="PLJ64_08440"
     CDS             complement(6407..7771)
                     /gene="ffh"
                     /locus_tag="PLJ64_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNC93549.1"
                     /translation="MFDQLSDRLQATLSDVRSRGKLTEADVDKAMREIRLALLEADVN
                     FKVVKAFTAKVKERCLGADVLGSLNPGQQVVKIVNEELTDLMGGAGRDLVFSNKGITV
                     ILMAGLQGSGKTTATAKLAMLLKKEGKSVALAACDVYRPAAIKQLETVGKRAGAVVYQ
                     QGTDADPVEIAAWALEKAKEEGRDVLIVDTAGRLHIDSELMDELARIRKRVKPHNVLL
                     VVDAMTGQDAVGVAESFSEAAEFDGVIISKLDGDARGGAALSVKAVTGKPIIFASTGE
                     KLEDFEHFHPDRMSSRILGMGDVLSLIEKAEEQVSEEEAEELQRKMSEGQFTLEDFLK
                     QMRQIRKMGPIGNLLGMMPGMGAMKNLKDVDVDDGELDRVEAIILSMTPHERAMPQII
                     KGSRRRRIAEGSGTKIQQVNNLVKQFDQMKKLMKHMANGQMPDQNQMAKMMGQTQRPK
                     IRRR"
BASE COUNT         1209 a         2745 c         2540 g         1389 t
ORIGIN      
        1 atcagggctg cttcatcaac ggttcgacaa ccggcctcca ccgcttccag caactcgact
       61 tcaaaatctt cgatcgccgg accgaacagg ggctggcctg cccccgcccc gagcatccgg
      121 tcgagcacgt cctgaccgcc acggatcagg gcggccccgt tggcgagcag ttcgttggtc
      181 cctgccgcgg ctccggcggt cacgggaccg ggcaccgccc ccacgtcccg gcccgcatca
      241 ctggccattt cggccgtgat cagcgagcca gaacgccagg ccgcttcaac caccacggtc
      301 atcgccgaga gcgctgccat gatccggttc cgggccggga agctccagcg ccaggcatca
      361 gccccgggcg gaacctcgga gatcaccaga ccactttcca ccacctgccg gtagagccgc
      421 gaatgtgagg ccgggtaggg ccggtctgcc ccgcagccga gcaccgccac ggtcggtccc
      481 cgctccagcg ccccgcgatg aacggcaccg tcgatgccca gcgccagccc gctgatcacg
      541 ttcagccccg cggccgccac ttcgcggccg atcgacgccg ctacctcaag cccgtagccg
      601 gtcgccttgc gggctccgac cacggtgacc gaattcccga gttcgatttc gtcgaggcga
      661 agcccgccgc cccggccgat cagggccacc ggcgaatcgg ccccgtcgtt gagaccggcg
      721 ggaaagctct cgtgatgcct gcagcaggcc cagcagccgg cgttctcgat cgcggccacc
      781 atctcgcctt cggtcaaaga tgcgttccag gcaaggatct tgtcggcctg cttggcggcc
      841 accgccgcga ccagcgtttc gctgtccagc cgcagcagtt ccggagtcct gcttcccgcc
      901 cggtcgtcaa ccaccctctg gatccaggcg ccgagccttg cgatcagcca gcttcgccgg
      961 agacattccg gacaggccga gcgggcgtca acccccgccc cgccaagtct tctcctcccg
     1021 ctcattgggc ggacctcctt cggaactgga ttgccgttgc catgtgctgc tcgccgatcg
     1081 cggtcgaacc ggccagatcc gcgaccgttc tcgcgacccg caggatcctg ccgtgagccc
     1141 ggccgctcag ctgccaggag cggtgtgcct ccgccagcag tccggcggcg gccgcggtga
     1201 ccttgaagtt cgccacctcg tccggctcgg cttccgagtt ggtgcgtcca tcccccagtc
     1261 tttccgccat cagctcgcgg gcaaggatca cccgcttcct gacctcgggc gattcttcac
     1321 ccggcggtcc ggccagggcc tcggccgaag gctgagtcac cgcgaccacg agatcgatcc
     1381 ggtccaccag cgctccgctg agtgccgccc ggtagcgacg ggccgacatt tcgctgcacc
     1441 ggcaccgtgg atcttcttca ccgcgaccgc aagggcacgg gttcgcagcc gcgaccagag
     1501 tgaaccggca gggcagggtc tgccggcccc tggccctggc gatcgtcacc gagcccgatt
     1561 cgaggggttc gcgaagcgct tcgagcgcgg gccggttgaa ctcgcagagt tcatcgagga
     1621 agaggacgcc gcgatgggca agggtgatct cgccgggccg tggcgggctt cccccgccaa
     1681 ccaggcccgc ggccgagacc gtgtgatggg gcgcccggaa gggcctggtc gagctgatcc
     1741 ggccatcacc gatcccgcag gcgctggcga tccttgcgac ctcgagaacc tcttcgccag
     1801 ccagtggcgg cagcaccgag ggcaggcgcc gggccgccag cgacttcccc gcgcccggcg
     1861 ggccgatcat cagcagcccg tgaccgccgg ccgccgagat ttcgaccgcg gctcgcagat
     1921 gcccctggcc acgcaggtcg gcaaggtcgg gcatctcgtc cgagcctccc ggcacggcgg
     1981 ggatcgccag tcttcccggg atctcccctc gttggaggct ggcgagctca ccgaaattgt
     2041 cgatcgtcag gaccgtgatc ccctccgcca gtgcggcctc gggaccgttg gcggtcggga
     2101 cgatcactcc ccgtcggccc tgctcgcgag cttcctcggc catcgccaaa gcgccccgga
     2161 ccgggcgaac cgaaccgtcc agggcgagtt caccaaccag tgaccagccc gccagcggtt
     2221 cgatcttgag atcacctgca gcgccgagca gggccgtcgc aagggcaaga tccatcgccg
     2281 gcccggcctt cctcaggtct gccggcgcga ggctggcggt gatccgctgc aggggaaaat
     2341 cgaagccgca gttgaccgtg gcggcccgga ccctctcgcg tgcttcccga accgccgcgt
     2401 cgggaaggcc gacgatctgg aagttcggca gaccgcggtg cacgtcgacc tccacccgga
     2461 tcatccgggc ggcgatcccg tcgagggtga atgaggtggt ctcggccagc atggggccaa
     2521 gctaggccga attcactcac atgtggctcg acgggtgcaa ccattccgca cgcctccgtg
     2581 aattccggac ggcaatgctc acacccgtgt gaattcactg caacaaaaga ccgttttcag
     2641 ccgcagatcg ccggtcccgg ccctactttc gaaggatgga cgaccgacga gccacaggac
     2701 gaagcgccga agacatctgc gcccgcagac tccgggaacg gggctggcgg atcctggccc
     2761 gcaactggcg aatccggccg ggcgagctcg acctgatcgc ccttgccggt tccaccctgg
     2821 tcatcgtcga ggtcaaggcc caccatcagg ggctgaggcg ggggccggtc catccggtcc
     2881 tggcggtggg ccccaacaag cagaggcgaa tcaggcggct ggcatcggcc tggattgccg
     2941 gccacgggcg aagcatcggg ttccgcgaag tccgcttcga cgtggtcgga atcacctttg
     3001 accgcgaagg caggctggcc gcctacgacc accttgaaga cgcattctga ggtgaagcgg
     3061 cgagccccgg ggcgatcgcg ctgccggtca ggcctgatac gcctcggaag cgaaggagag
     3121 ccggtggatc ggcgagggtc cgagcagcct gatcgcgtcc cggtgagcgg gtgatgcgta
     3181 ccccttgtgg gcttcgaagc cgtagccgtc gtaggccagg ccggcccggg ccatgcagcg
     3241 gtcacgggtg accttggcga ggatcgacgc ggccgcgatc gcggcgctgg tgctgtcgcc
     3301 cttgacgagc cgccggtgcg gcacctcgca acccttgagg gcgaacccgt cgaccagggt
     3361 caccgcttcg ggtccggagc ccagcccttc gatcgcccgg gccaggcatt ccatgttgct
     3421 tacgtgaagt ccgttggcgt cgatgtacct ggccgagcgc atcaccacga ccgtcttcac
     3481 ggcgcagtcc agcacttcgg ggtagagccg ctcgcgggcc ttggcggtca gcttcttcga
     3541 gtcccggatc tccgccagcc gcccgccgcc ctggctctcg aggcattcgc ggtcgaaaag
     3601 gaccgccgcg gccacgatcg ggcctgcaag gcaaccccgg ccggcctcgt cggccccggc
     3661 gacggcggaa gcacccagtt cgaggtcgtg actgaacagc tcccccggag cctgttccgc
     3721 cgcgggagtt cgggcggtgc tagagcccac cgattcggtt cggcggccag taggtagcga
     3781 aggccttgcc gatcagccat tcttcgggca agggacccca gaaacgactg tcgtcgctgt
     3841 gtccgcggtt gtcgcccatc atgaagaagt gaccttcggg aacggtgatt tccttcggca
     3901 tgttgcagcc ggtgccggcc ggcccgcagg ggttgatctc gtagccgtcc ggctcgacca
     3961 cctcgccgtt gacgacgggc aggccctttt caatcgagac cgtgtccccc ggaccggcga
     4021 cgattctttt gatgaaggtc tcgctcgact tgtcggaggt cggctgagga cagggctggt
     4081 cctgctgatg tggggcgcca cattcctcga ggccgccccg gtcgactccg gctggcggat
     4141 gaaagacgac gacatcgccc ggcttggggt cggtgaagtg gtagacgaag cggttgacca
     4201 ggatccgctg gttgatgtcc agggtcggct ccatcgagcc agacgggatc tggtacggct
     4261 tgatcaggaa ggcctggatc agcagcgcca gcccgagggc gagcgcaacg attacgacca
     4321 gttcgacgaa gctacccccg gccgttttcg gcttggggat cttcgggctc aggcttcttc
     4381 cttctcgccc tgctcgccgt cttcggcggg ctcttcagtt gcttcggcct caggagcctc
     4441 ttcggcagct tcggcttccg gcgcctcatc ggcctcggca gcgggctcct cggcgggagc
     4501 ctcttcggct tccccggccg gcgcttcctc cgcggccggc tcctcggcgg tagcttccgg
     4561 agcttcggcc ccggtcgctt cggcggtttc ggtcgcttcg gcggtttccg tggcttctgc
     4621 ctgagcagcg tccgactccg tggcctctac ttcggtcgct tcctcgatct cctcgaggga
     4681 ttcacccgaa gcgtccacgg ccttggatgt gccgaccatg tcctcgtcaa tgccccagcg
     4741 acgctcggca acccgggcgg ccttgccgac ccgaccgcgc aggtagtaga gcttggcccg
     4801 gcggacccgg ccacgcgagg cgacttcaag cttctcgatc ttcggcgagt ggagcgggaa
     4861 agtacgctcg acaccgacgc cgaaggactg cttgcgaaca gtgaaagtct cgcgggcacc
     4921 ggagccctga cgcttgatca ccacgccctc gaatacctgc gggcgcttgc gatttccctc
     4981 gatcacgagg aagtgaacgc gaacgcgatc tcccggggaa aaggacggaa gtcccttcct
     5041 cagctgactg ttctcaatgt cctggattac ggtgctcatg gctcaaaaaa gcggacccgg
     5101 ttgaaggccc gcggacgcgc aatcctaccg aagcagagca ggcagcgtca ttccgccccc
     5161 cgcttgcggc tttgctcgag tcgccactcc ctgacttttg cgtgatcgcc ggagatcaac
     5221 acgtcgggga tgccccagcc ccggtactcg ggtggacggg tgtagtgcgg gtattcgggc
     5281 agcccttcga gggcctcgga ccaggattcc tcgatcgccg attcctcgtc gccgagggcg
     5341 ccgggctggc gccgcatgat cgtgtcggca agcaccattg ccggcagctc gccaccggcc
     5401 agtacgtagc ggccgatcga aatctcgtcg tcggccaggt gttcgtggac ccggtggtcg
     5461 aaaccctcgt agcgaccgca gagcagggca aggcccggtt cggccgcgag ttcggtgacc
     5521 aggccatcgt cgagtacccg gccggccggg ctgagaacca cggtcctggg cctttcgccg
     5581 tcaccgtaga caccccggag ggcggcgtcg accacgtcga cccgcaatac cattcctgcc
     5641 ccgcctccgt aaggggcgtc atcgacccgg ccacccttca ggggagtcca gtcgcggtaa
     5701 tcccgaaggt tcagctccgc gccgtgctcg atcgccttgc ggaccggccg ctgctgccga
     5761 aaccagtcaa aggcctcggg aaagagggtg aagatgtcga gattcatcga cctgcttccg
     5821 ggccgggtat gcggccggac cgcatcggct tcagtccagg atgtcgacca gcacgcggcg
     5881 ctcttcgcgc acggcggcgg ccttggcgat ggtgcggatc gcgttggcga ccctgccacc
     5941 ccggccgatc acccggccga gatcgtcttc gccgaccgtg atctcgaaga ccaggtcgcc
     6001 gtcttcctcg agttcctcga cctcgactgc gtcgggatcc tcgaccaggg cccgcgtgat
     6061 gtagagaagc atctcctgca tcggcgggaa gagcctcagc tgccggtggc cttgatgccc
     6121 ttgatgcgca gcaggccggc gacccgctcc gagggctggg cgcccttttc gagccagtaa
     6181 ttggcccgtt cctcgtcgat cacgatggtc gacggatcgg tctgggcgtt gtactggccg
     6241 atcgtctcga tggtgcgacc gtcacgcggg gcacgcttgt cggcgacgac gattcgccag
     6301 atcgggttct tcttcgaacc aactcttcta agtctgatgc ggactgccat tggacgcgga
     6361 atcgtacagg tttggcgggc ttacagtcgt gtggcgggca acttggctag cggcgccgga
     6421 ttttcggtcg ctgggtctgg cccatcatct tcgccatctg gttctggtcc ggcatctggc
     6481 cgttggccat gtgcttcatc agcttcttca tctggtcgaa ctgcttgacc aggttgttga
     6541 cctgctggat cttggtcccg gagccttcgg cgatccggcg gcggcgcgaa cccttgatga
     6601 tctgcggcat cgcccgctcg tgcggggtca tcgagaggat gatcgcctcg acccggtcga
     6661 gctctccgtc atcgacatcg acgtccttca gattcttcat cgcccccatg ccgggcatca
     6721 tcccgagcag gtttccgatc ggtcccatct tgcggatctg gcgcatctgc ttgaggaagt
     6781 cctcaagggt gaactgcccc tccgacatct tccgctgcag ctcttcggct tcctcttcgc
     6841 tgacctgctc ctcggccttt tcgatcaggc tgagcacgtc gcccatgccg aggatccggc
     6901 tggacatccg gtcgggatgg aaatgctcga agtcctcgag cttctcgccg gtcgaggcga
     6961 agatgatcgg cttgccggtg accgccttga ccgaaagggc ggcgccgccg cgggcgtctc
     7021 cgtcaagctt cgagatgatc accccgtcga actcggctgc ctcgctgaac gattcggcca
     7081 ccccgaccgc gtcctgaccg gtcatcgcgt cgaccaccag cagcacgttg tgaggcttga
     7141 cccgcttgcg gatgcgggcc agctcgtcca tcagttcgga gtcgatgtgg aggcggccgg
     7201 cggtatcgac gatcaggacg tcgcggcctt cctccttcgc cttctcgagc gcccaggcgg
     7261 cgatctcgac cggatcggcg tcggttccct gctggtagac gaccgcgccg gcccgtttac
     7321 cgacggtttc cagctgcttg atcgcggccg gccggtagac gtcgcaggcg gccagcgcga
     7381 cgctcttgcc ttccttcttc agcagcatcg ccagcttggc ggtggcggtg gtcttgccgg
     7441 aaccctgcag gccggccatc aggatgaccg tgatcccctt gttcgagaag acgaggtcgc
     7501 gcccggcccc gcccatcagg tcggtcagct cctcgttgac gatcttgacg acctgctggc
     7561 ccgggttgag cgagccaagc acgtcggcgc cgaggcagcg ttccttgacc ttcgcggtga
     7621 atgccttgac gaccttgaag ttgacgtcgg cctcgagcag ggcgaggcgg atctcccgca
     7681 tcgccttgtc aacgtcggct tcggtcagtt tgccgcggct ccggacatcc gagagagtcg
     7741 cctgcagccg gtctgaaagc tgatcaaaca tgggccacgg agcctaccga ccaacctcca
     7801 gcaactgtgg ttacgccgtg ggccagcagg ggtcaccggg aagcggcgaa cacttcgcgc
     7861 ggcattcagc cgcgctctgc ctg
//
LOCUS       DAOQKN010000118         7878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_4495_length_7878_cov_5.350249, whole genome shotgun sequence.
ACCESSION   DAOQKN010000118 DAOQKN010000000
VERSION     DAOQKN010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 7878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7878
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4495_length_7878_cov_5.350249"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            507..716
                     /locus_tag="PLJ64_08445"
     CDS             507..716
                     /locus_tag="PLJ64_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93550.1"
                     /translation="MAREVFDQARPRSTKAQRFADAFKGCEEWTVPPRKPRSMSDSVF
                     NAEESPFDSRIYGAEIIDLEFREAA"
     gene            complement(720..1376)
                     /locus_tag="PLJ64_08450"
     CDS             complement(720..1376)
                     /locus_tag="PLJ64_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93551.1"
                     /translation="MNQTGRVQGKDGRERRRLPSPALVLSLLALFVALGGTATALKGK
                     WSVKNDDIAPRAVDAGKVKLGSINGHHLADGVVGPWKLKLGATAVTSGESSTTSTVPV
                     DLGGPTATVKVPEGGMVAIQAEAMMRATGSNAARVYLYDPLSVPNPVMIHRSEANDFD
                     LRISTPGVGASDGVTSRVRAGWIVMPVTAGTHSFQLRYSTSGGTAIFKDRVLRVAVIR
                     "
     gene            complement(1598..3295)
                     /locus_tag="PLJ64_08455"
     CDS             complement(1598..3295)
                     /locus_tag="PLJ64_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron-transfer flavoprotein:ubiquinone
                     oxidoreductase"
                     /protein_id="HNC93552.1"
                     /translation="MPPVAPSEYPPPFDSESVITAPTDPEDERIEVGVAIVGGGPAGL
                     AAAVKTLQLLEDKPELMEKLGEVPVAVVEKGKVCGAHGMSGANMRPSAMEELFPDLPR
                     DEWPVYREVDKDAVYLLTEKLALPLKPMPPNFKNHGNYVTSVSKLSRFLAEKAEEMGA
                     YILTETTADKLLVEDQIVRGIRSGDRGRGRQGQELGNFEPGMDLIAKGTVLAEGVVGH
                     LTYAALDHFDINGLDPQRFELGVKEIWEVEEPLDQVIHTMGWPLRKAAKYKEFGGSFI
                     YPMEDNKICIGFVAGLDYTDATFSVHDVLQQFKQHPFVKKILKGGKRVGWGAKTIPQG
                     GYWSMPKRLSVPGMVIAGDNAGMVNVAELKGVHYAMHAGMYAAEAIVDALEKDQVDFS
                     AYDEKVHNSIIEKDLYKTRNASQPFTRGFFFGGAMASTMTLTQGHFPGGHWKNHDDAT
                     YKMEIGKASESYPEPDGEYTFDKLSSVFLSGNATRDDAPNHVRIQTEVPREIAQTWVS
                     MCPAQVYEIPEDQLESDAPIVDVHVTASNCVQCGAITAKGGRLTLPEGGDGPLYTET"
     gene            complement(3404..4378)
                     /locus_tag="PLJ64_08460"
     CDS             complement(3404..4378)
                     /locus_tag="PLJ64_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HNC93553.1"
                     /translation="MAGILVYALHDDEGNFNKNSLGALSEAAKLAGELGTEAAAVIVG
                     DISDDGAAGLGNYGVTKVYRAKDVPEGLAQPIVDVMAKVIQDNGYTYALFGGGLLGFE
                     IGAGLTARLGAGVTMEVTKVKVDGGKLVSERPILQDSKIADVGYVSEPGIIIGRLNAF
                     DVNESGGTAEVVDVDVELSEFSTKAEMITRGEQRGADVDIEAADILIGGGRGLGSADG
                     FKDCEELAGAFSNGAVAATRAVVDAGWYPYAAQIGQTGKTVAPKLYLAVGVSGAIQHK
                     VGMQNSENIVAINKDANAPIFEFSDLGIVGDLNKIMPKLTEAIKAKKG"
     gene            complement(4379..5170)
                     /locus_tag="PLJ64_08465"
     CDS             complement(4379..5170)
                     /locus_tag="PLJ64_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNC93554.1"
                     /translation="MKICVLVKDVPDAAVQKRIDPGTMRLDRSGENTLNPFDTHAIEA
                     AMQLREGGELQVDEIVAVTMGTDTAARALQKAVALGADRSVHLSDDALAGSDVCATGL
                     ALAKVLESEGPDLVLLGQQSDDGECYTIGAVVADHLKMPSLTQVIDMKVDGDKIRCER
                     QAEYGYDTVALTLPAVISVGDAINEPRYPSLKAIMGAKKKPLDSKSAADVGIDAGQVG
                     EAGSQTTVISLNEPPQKEAGEIIEDEDTAETVEKIVAWLEERKLI"
     gene            complement(5395..6054)
                     /locus_tag="PLJ64_08470"
     CDS             complement(5395..6054)
                     /locus_tag="PLJ64_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93555.1"
                     /translation="MIELLLPGAVPDFVREAQSILREGSTFQWSTVTLLGLVVYVYSV
                     EVERRRWDIVLAGLAFSMMDLFNEMVNSAIFHLTDRAPLWTVTGDTSYLIFIGLTIEI
                     LLLFAVSGVAFVKTLPPDRKAKILGIPNRIVFVTVFSLFCVAVEIFLHSTGYFHWEYW
                     WWNVPFVPLIVIFGYATFFAVAAWVYDMGSNTRKQVRVVGTMAAINLVLGLALGLAGW
                     L"
     gene            complement(6051..>7878)
                     /locus_tag="PLJ64_08475"
     CDS             complement(6051..>7878)
                     /locus_tag="PLJ64_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93556.1"
                     /translation="LGLSFFALLLPGHSAAARAAGECTLIPGGTIGDPEPEPVPDRAG
                     SPTDRGPGAHGANPTLKGLPTRVDLRSPSMAQNRNHYFALKNGRLFVKPNYERTGRRG
                     SWRHVTTPSCLDGDIRSIDADDDELTAINSQNQIFGMDQALSSPSRFNWTSRWGFPIW
                     LGDGYTIPKDTVAWAWTVISPRESRYWVDPAGNQHTVGEGKVSHVLSLRGDHRRITMN
                     DPWLPQDRSYEVCGPRRGTFRISNLAASGSVLFVIGDHGDMYTRLWDFDIAGLDNAFF
                     DYSYEDQRGVEDPAIQLPGAHWVQQPKIRGEITNRISITTIGRGTLHRILRVEGKNAK
                     GLPGFWQKDLYTLNRGSSGWKFVATPKAPITGRWLDNSPRDMGAVGAGRPEGRRYLAS
                     GANYLAEIPDFNVSCGRALLRVSFDDGRSIDLNLFLDDMVRIYPRARGLDGEPRLVLG
                     TIAVTPRARATGGDEARDFIRDVLGDRKFTTARITATTAGLEIPDLGLQFTAAGEPSA
                     GRVPDQGFKEFHVMGRKLGLAFTFETATESPASIVVSRIDGDGRGPVAARLTRTGSFF
                     WEPRRLADGRYRVEASSMGLGGRLATRSVFVRRKQGHFSVIG"
BASE COUNT         1328 a         2514 c         2610 g         1426 t
ORIGIN      
        1 cccccacccg accccacggc tacacgaacc cgggagaaac ctcaacgacc cccccgcggc
       61 tacacggacc cgggagaaac ctcgacgacc ccactcgacc ccgcgctaca cggacccggt
      121 gctggtcgac ggggttgcgc tggccttccg gaggcgattc gcgagagtcc acttttggcc
      181 acatggcgca ctcaactccg atctcgaatc ggcggcccgg atcgaaatcg gagttgggtg
      241 cgctatttgc cgataactcc cgcctcgaat ccgcccgaga ctccgaccca ctcccccttc
      301 accagccctg ccgacttcga ggtgccgcag atttgcgacc tctggggtcg taaacctgcg
      361 gcaccggccg cggctaacgg agcggaaagt gcggcgcgga ctcgtggcgc cgcgggaatt
      421 tcgcaaattg caagaaaatt ggcaggtttt ccgtccgatg gggcgaacta gctttccata
      481 accccttccg gaggaggatt gtccgtatgg ccagagaggt attcgaccag gctcgccccc
      541 gtagtaccaa ggcgcagcgg tttgccgatg ctttcaaggg ctgcgaagag tggaccgtcc
      601 cgccgcggaa gccccgatcg atgtccgatt ccgtgttcaa tgccgaagaa tcgccgttcg
      661 attcacgcat ttacggagcc gagatcatcg acctggaatt ccgcgaagca gcctgagcac
      721 tatctgatca cagccaccct caggacgcga tccttgaaga tcgcggttcc gcctgacgtg
      781 ctgtagcgca gctggaagct gtgggttcct gccgtcaccg gcatgacgat ccatcccgcc
      841 ctgacccggc tggttacccc gtccgaagct ccgacgcccg gcgtcgagat cctcaaatcg
      901 aaatcgttgg cttccgagcg gtggatcatc accggattcg ggaccgagag cgggtcgtag
      961 aggtagaccc gcgcggcgtt gctgccggtg gccctcatca tcgcctcggc ctggatcgcg
     1021 accatcccgc cctccggcac cttgaccgtg gcggtcgggc cgcccaggtc aaccggcacc
     1081 gtcgaagtgg ttgacgactc tccggaggtg acggcggtcg cacccagttt gagcttccag
     1141 gggccaacca ccccgtcggc cagatggtgg ccgttgatcg agcccagctt gaccttgccg
     1201 gcgtccacgg cccttggcgc gatgtcatcg ttcttcaccg accatttgcc cttgagcgcg
     1261 gtggcggtac cgccgagcgc cacgaacaaa gcgagcagcg agaggaccag cgccggcgat
     1321 ggcaacctgc gcctttcgcg tccgtccttc ccctgcaccc tgcctgtctg attcatcatc
     1381 cctccctcgg gaatcgtgag tgttggttca cagctacccg ggcgggaccg gatgaaaccg
     1441 gcatccccag gcggcgcacc cgaacccggg caccaaagag aagcgacgcc ggatttctcc
     1501 ggcgtcgcct tgaactgctt cgggccgtga agcccgggcg cttgatctcc aggccggaga
     1561 ccagccgtga cggctgaccc cctgtccggc ctctggatca ggtctcggtg tagagcgggc
     1621 cgtcgccgcc ttccggcagg gtcaggcggc cgcccttggc cgtgatcgcg ccgcactgga
     1681 cgcagtttga agcggtgacg tggacgtcca cgatcggagc atccgactcg agctggtcct
     1741 cggggatctc gtagacctgc gccgggcaca tcgaaaccca ggtctgggcg atctcgcgcg
     1801 ggacctcggt ctggatccgg acgtgattcg gcgcgtcgtc acgggtcgcg ttgcccgaga
     1861 ggaagaccga cgacagcttg tcgaaggtgt actcgccatc cggctccggg tagctctcgg
     1921 aagccttgcc gatctccatc ttgtaggtgg cgtcatcgtg gttcttccag tgaccgccgg
     1981 ggaagtggcc ctgggtgagg gtcatggtcg aagccatggc gccaccgaag aagaagcccc
     2041 gggtgaacgg ctggctcgcg ttacgggtct tgtagaggtc cttctcgatg atcgagttgt
     2101 ggaccttctc gtcgtaggcg gagaagtcga cctgatcctt ttcgagggcg tcgacgatcg
     2161 cttcggcggc gtacatgccg gcgtgcatcg cgtaatggac acccttgagc tcggccacgt
     2221 tgaccatgcc cgcgttgtcg ccggcaatga ccatgcccgg gaccgaaaga cgcttcggca
     2281 tcgaccagta accgccctgc gggatcgtct tggcgcccca gccgacccgc ttgcctccct
     2341 tgaggatctt cttcacgaag ggatgctgct tgaactgctg caggacgtcg tggaccgaga
     2401 aggtggcgtc ggtgtaatcg agaccggcga cgaagccgat gcagatcttg ttgtcttcca
     2461 tcgggtagat gaacgaaccg ccgaactcct tgtacttggc ggccttgcgg agcggccagc
     2521 ccatcgtgtg gatgacctgg tcgagcggct cctcgacttc ccagatctcc ttgacaccga
     2581 gctcgaagcg ctgcggatcg aggccgttga tgtcgaagtg atcgagcgcg gcataggtga
     2641 ggtggccgac cacgccttcg gcgaggacgg tgcccttggc gatcaggtcc atgcccggct
     2701 cgaagttgcc gagctcctgg ccctggcggc cgcggccgcg gtcaccggaa cggattccgc
     2761 gcacgatctg gtcctcgacc agcagcttgt cggcggtggt ctcggtcagg atgtaggcgc
     2821 ccatctcctc cgccttttcg gcgaggaagc gggaaagctt ggaaacggag gtgacgtagt
     2881 tgccgtggtt cttgaagttc ggcggcatcg gcttgagcgg aagcgcgagc ttctcggtca
     2941 gcaggtagac cgcgtccttg tccacctccc ggtaaaccgg ccactcgtcc cgcggcaggt
     3001 cggggaagag ctcttccatc gccgaggggc gcatgttggc gccggacatg ccgtgggcgc
     3061 cacagacctt gcccttctcg acgaccgcga caggaacctc gccgagcttc tccatcagct
     3121 cgggcttgtc ctcgagcagc tggagggtct tgacggcagc agcgagaccg gccgggccac
     3181 cgccgacgat tgctacgccg acttcaatgc gctcatcttc gggatcggtg ggcgcggtga
     3241 tgacgctttc ggaatcaaag gggggcggat attcggaggg cgctacgggc ggcatgctct
     3301 tggtcctttc gggacgaatc ggtggtgaga cgagaagttc ttcaatcggg gtgaaactac
     3361 gggccgggga agctttgacg ctcccccggc cggatactgc ttgctagccc ttcttggcct
     3421 tgatggcctc ggtgagcttc ggcatgatct tgttcagatc gccgacgatg ccgaggtcgg
     3481 agaactcgaa gatcggggcg ttcgcgtcct tgttgatcgc cacgatgttt tcggagttct
     3541 gcatgccgac cttgtgctgg atggcgccgg agacgccaac cgccaggtac agcttcggcg
     3601 cgaccgtctt gccggtctgg ccgatctggg cggcgtaggg gtaccagccg gcgtcgacca
     3661 ccgcgcgggt tgcggcaacc gcaccgttgc tgaaggcacc ggcgagttcc tcgcagtcct
     3721 tgaagccgtc ggccgagccg aggccacgac cgccaccgat caggatgtcg gcggcttcga
     3781 tgtccacgtc ggcgccacgc tgctcgccgc gggtgatcat ctcggccttg gtcgagaact
     3841 cggagagctc gacgtcgacg tcgacgacct cggcggttcc gccgctttcg ttcacgtcga
     3901 aggcgttgag gcggccgatg atgatgccgg gctcggagac gtagccgacg tcggcgatct
     3961 tcgagtcctg caggatcgga cgctcggaga cgagcttgcc accgtcaacc ttgaccttgg
     4021 tgacttccat ggtcacgccg gcgccgaggc gggcggtcag gccggcaccg atctcgaagc
     4081 cgagcaggcc gccgccgaac agggcgtagg tgtaaccgtt gtcctggatg accttggcca
     4141 tcacgtcgac gatcggctgg gcgaggccct ccggaacgtc cttcgcgcgg tagaccttgg
     4201 tcacgccgta gttgccgaga ccggcagctc cgtcgtcgga aatgtcgccg acgattacgg
     4261 ctgcggcttc ggtgccgagc tcgccggcca gcttggcggc ctcggagagg gcgccgagcg
     4321 agttcttgtt gaagttgccc tcgtcatcgt gaagggcgta aacgagaatg ccggccattt
     4381 agatcagctt cctttcctcg agccacgcaa cgatcttctc gaccgtttcc gcagtgtctt
     4441 cgtcttcgat gatctcgccg gcttccttct ggggcggctc gttcagcgaa atgacggtcg
     4501 tctgtgagcc ggcttcgccg acctggccgg cgtcgatgcc gacgtcagcg gcggacttgc
     4561 tgtccagcgg cttcttcttg gcgcccatga tggccttgag gctcgggtaa cgcggctcgt
     4621 tgatcgcgtc accaaccgag atcacggccg gcagggtcag ggccacggtg tcgtagccgt
     4681 actcggcctg acgctcgcag cggatcttgt cgccgtcgac cttcatgtcg atgacctggg
     4741 tcagcgacgg catcttcagg tgatcggcca caacggcacc gatcgtgtag cactcgccgt
     4801 catcggactg ctggccgagc aggaccaggt ccgggccctc ggactcgagg accttggcca
     4861 gggcgaggcc ggtggcgcag acatcggagc cggcgagggc gtcgtcggac aggtggaccg
     4921 aacggtcggc gccgagggcg acggccttct gcagggcgcg ggcagcggta tcggtgccca
     4981 tcgtcacggc gacgatttcg tctacctgga gttcgccgcc ttcgcgaagc tgcatcgcag
     5041 cctcgatggc gtgggtgtca aaggggttga gagtgttctc gccgctgcga tcgagccgca
     5101 tggtgccggg atcaatgcgc ttttgtaccg cggcgtctgg cacatctttg accaggacgc
     5161 aaatcttcaa ctgaagcctc cttagggggt cttacgaggg gctctgccga attcaagggc
     5221 ggagagccgg agatgctgtt ttgcgacgcg ggattctatc gggattcgcc aaaagttgac
     5281 tgacgagtca atcagagggc ttctgcagtc gggcgggtct tcacccgccc tccggcctgg
     5341 aacccgcccc tcccactcga agtcaatgtt tgtcgggagg ggatcaggga gtccctacaa
     5401 ccaaccggcc agtcccaatg ccaggcctag aaccagattt atggcggcca tggtgcccac
     5461 caccctgacc tgtttccggg tgttcgatcc catgtcatag acccaggcgg cgaccgcgaa
     5521 gaaggtggcg tagccgaaga tcacgatcag cgggacgaag ggcacgttcc accaccagta
     5581 ctcccagtgg aagtagccgg tgctgtgcag gaagatctcg accgccacgc agaagagcga
     5641 gaagacggtc acgaaaacga tccggttcgg gatcccgagg atcttcgcct tccggtcggg
     5701 cggcagggtc ttgacgaagg caaccccgga aaccgcgaag agcagcagga tctcgatcgt
     5761 caggccgatg aagatcaggt agctggtgtc accggtcacg gtccagagcg gcgcccggtc
     5821 ggtcaggtgg aagatcgccg agttgaccat ctcgttgaag agatccatca tcgagaaggc
     5881 gagcccggcc agcacgatgt cccagcgccg gcgttcgacc tcgaccgaat acacgtagac
     5941 gaccaggccg agcagggtga ccgtggacca ctggaaggtg gatccttcgc gcaggatgga
     6001 ctgggcctcc cggacgaagt cggggaccgc cccgggaagc agcaactcga tcatccgatc
     6061 accgagaaat gaccctgctt tcgccgcacg aacaccgagc gggtggcgag ccggccgccg
     6121 agacccatcg aactggcctc gaccctgtac ctgccgtcag ccagccgccg cggctcccag
     6181 aaaaacgacc cggtgcgggt gaggcgggct gccaccggtc cgcgaccgtc accgtcaatc
     6241 cggctgacca caatcgaagc cgggctttcc gtcgccgtct caaaggtgaa ggcgagcccc
     6301 agcttccgac ccatcacgtg gaactccttg aagccctggt caggtacccg tccggccgaa
     6361 ggttccccgg cggctgtgaa ctgaaggccg aggtccggaa tctcgagccc ggcggtcgtc
     6421 gcggtgatcc gcgcggtggt gaatttgcga tcgccgagca cgtcccggat gaaatcccgg
     6481 gcttcgtcac ccccggtcgc ccgggcccgt ggcgtgaccg cgatggttcc caggacgagc
     6541 cgtggttcgc cgtcgaggcc gcgggcgcgc gggtagatgc ggaccatgtc gtcaaggaag
     6601 agattcaggt cgatgcttct tccgtcgtcg aagctgaccc gaagcagggc ccgcccgcag
     6661 gaaacgttga aatccgggat ctcggccagg tagttggctc cgctcgcgag gtaacgccgg
     6721 ccctcgggcc ggcccgcccc gaccgcgccc atgtcccggg gcgaattgtc gagccagcga
     6781 ccggtgatcg gcgccttcgg ggtggcgacg aacttccagc cggacgagcc ccggttcagg
     6841 gtgtagaggt ccttctgcca gaacccgggc agtcctttcg cgtttttgcc ctcgacccgg
     6901 aggatgcggt gcagggtgcc ccggccgatc gtggtgatgc taatccggtt ggtgatctcg
     6961 ccccggatct tcggttgctg gacccagtgg gcgccgggca gctgaatcgc cggatcctcg
     7021 accccgcgct ggtcctcgta ggagtaatcg aagaaggcgt tgtcgaggcc ggcgatgtcg
     7081 aagtcccaga gccgggtgta catgtcgccg tggtctccga tcacgaaaag caccgaaccc
     7141 gaggcggcaa ggttgctgat ccggaaggtt ccgcgacggg ggccacagac ctcgtaactc
     7201 cggtcctgcg gcagccaggg gtcattcatg gtgatccgcc gatggtcccc gcgcaggctg
     7261 agcacatgcg agaccttgcc ctcaccgacc gtgtgttggt tgccggccgg gtcgacccag
     7321 tacctcgatt cccgcggcga gatcacggtc caggcccagg ccacggtgtc cttggggatc
     7381 gtgtagccgt cacccagcca gatcgggaag ccccagcggg aggtccagtt gaagcgggag
     7441 gggctcgaaa gcgcctggtc catgccgaag atctggttct gcgagttgat cgcggtcagc
     7501 tcgtcatcgt cggcgtcgat cgagcggatg tcgccgtcca ggcacgacgg ggtggtgacg
     7561 tgccgccagc tcccgcgcct gccggtccgc tcgtaattcg gtttgacgaa gagccggccg
     7621 tttttgaggg cgaagtagtg attgcggttc tgggccattg acggcgagcg caggtcgaca
     7681 cgggtcggca ggcctttcaa ggtcgggttg gcgccgtggg cgcccggacc ccggtcggtc
     7741 ggagaaccag cccggtccgg gaccggctca ggctcggggt cgccgatcgt gccgcccggg
     7801 atgagcgtgc attcccccgc cgccctggcg gccgctgaat ggccgggcag cagcagggcg
     7861 aagaaggaca gccccagg
//
LOCUS       DAOQKN010000119         7844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_4519_length_7844_cov_4.062781, whole genome shotgun sequence.
ACCESSION   DAOQKN010000119 DAOQKN010000000
VERSION     DAOQKN010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 7844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7844
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4519_length_7844_cov_4.062781"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /gene="pdxS"
                     /locus_tag="PLJ64_08480"
     CDS             complement(<1..694)
                     /gene="pdxS"
                     /locus_tag="PLJ64_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HNC93557.1"
                     /translation="MAEEATFRVKTGLAEMLKGGVIMDVVDADQAKIAEDAGACAVMA
                     LERVPADIRRDGGVARMSNPRMIKEIQDAVSIPVMAKVRIGHFVEAQVLQSLSIDFID
                     ESEVLTPADETNHVDKFGFDAPFVCGATNLGEALRRIGEGAAMIRSKGEAGTGNVVEA
                     VRHMREIVGGIKRLSTLSSTELPTAAKELQAPLELVRDVATRGWLPVPVFCAGGIATP
                     ADAALMMQLGAEG"
     gene            complement(773..>1222)
                     /locus_tag="PLJ64_08485"
     CDS             complement(773..>1222)
                     /locus_tag="PLJ64_08485"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNC93558.1"
                     /translation="VAALSGASQVVIVGTDQMAFFASYLRHTLSLLDIRAEIVTSTAG
                     PSLQRLGRVDEETLLITLSSGRSHALLARAMKLAGHRGARTLAIADATLSDAVKKADM
                     SLYFSSNSPSFLRSNTALMALVHALAYGVYSLSEDEHSERIRAYKLK"
     assembly_gap    1223..1322
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1459..1794
                     /locus_tag="PLJ64_08490"
     CDS             1459..1794
                     /locus_tag="PLJ64_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93559.1"
                     /translation="MNQFQGLPNRKTSLTGHTDEGDEIWIIRSISQKFYKCLGCKGEI
                     NIGDEHVVVQYVGKYGGTEHSHWHQRCAEEILFYQVRGMRKVSSKESSRDRLESRGRR
                     PAGRRRRPR"
     gene            complement(1850..2545)
                     /locus_tag="PLJ64_08495"
     CDS             complement(1850..2545)
                     /locus_tag="PLJ64_08495"
                     /inference="COORDINATES: protein motif:HMM:NF019599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1775 domain-containing protein"
                     /protein_id="HNC93560.1"
                     /translation="MKKVGSFLVLLAVLLATPVFAGVASAHVEMTPDEAPAGKPVDLS
                     FEIPHGCDGAATTSIVVQIPKAAADVKAGNVKGWKATSAGNTLTWTGGPLPDHQTQDF
                     PFTVTLYGKKDENVMFKTIQKCQGDASTAWIQAMEGDVEPEYPAPAVTLTSTAKAQVS
                     PATPADQQSDDDQVAEGDAAATGEPTATAADAEDSSSDDEGSDSKTLILIIIAGLAIG
                     TVAGIVVRARRKQ"
     gene            complement(2555..3244)
                     /locus_tag="PLJ64_08500"
     CDS             complement(2555..3244)
                     /locus_tag="PLJ64_08500"
                     /inference="COORDINATES: protein motif:HMM:NF019599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcnI family protein"
                     /protein_id="HNC93561.1"
                     /translation="MNIKRKIILGLAALFVLAIPATASAHVTVSPEEAPAGDYAMLTF
                     TVPHGCDGAATTSVKVQMPPQVVAATPGVVPGWNIKTVEGKLPQPSEMHGEKITEGVR
                     QVTWTGGPLADDQLQQFPLSVAFAGAEGDAAEFKVIQGCEGGAETAWIQSTPASGEEP
                     EHPAPTVMLTAASEEGHDHMAAADDSAEAASADSSDDDGSDNGLAIAALIVGALGLVV
                     GSVALVGSRRK"
     gene            complement(3298..3549)
                     /locus_tag="PLJ64_08505"
     CDS             complement(3298..3549)
                     /locus_tag="PLJ64_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93562.1"
                     /translation="MSHWLTGFLPACAPLLLLVLLLLLGRYPGERAIERVHRALSDWL
                     GHSFGGRVVEPQIVFVPVPDRGGRLIARSLAGRAPPCFI"
     gene            complement(3546..5273)
                     /locus_tag="PLJ64_08510"
     CDS             complement(3546..5273)
                     /locus_tag="PLJ64_08510"
                     /inference="COORDINATES: protein motif:HMM:NF017262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HNC93563.1"
                     /translation="MRRSLLTSLLFLACLLGLASSASAHAVLEGSSPTRGAELNSSPG
                     HVDFTFNEPVEASLGAVRVFNTSGDQVQTDDLERPGENADAVGASLPADLPDGLYTAT
                     YHVISADSHPVSGGITFTIGKPGKDASAFTQGKTISELLAESEAGKVTEVAFWADRWI
                     GYLAIALAVGALAWMLLIWGPLSNTPGIDRRVIERRFSRLILVVGLAGLLASLMAIIF
                     QGAIGAGTSFWRAFGSGIPGEVIHTRFGTVMLIRSAAWLALIGLVLAGRGRYTRPGPL
                     TILACLAGLVLAASPAFAGHASTRDPAWLLVPSDIIHAVAMAIWTGGLVAMLALLPAA
                     TRQLPEPSDRTGLLTRATRSFSAVALAAVSMISVSGAVQAIIEVGSVPDLVETQFGRA
                     VSIKIVLFGILIGLGFINRNRIIPGLVRRLERSESPGAPGNRLRSSLRIEVILVTVVL
                     GVSAALVSYPPPDAIQSGPASGAVEIQGKRVEYTVDPARVGSNEVHLYVFDDRTGAPV
                     PVRSMEVSFSLPAEDIAPIEADARRAGPGHFVVPSAMLGVQGEWRAEVAIRLSEFDEP
                     IAEFEVEVK"
     gene            complement(5270..5992)
                     /locus_tag="PLJ64_08515"
     CDS             complement(5270..5992)
                     /locus_tag="PLJ64_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transmembrane domain-containing protein"
                     /protein_id="HNC93564.1"
                     /translation="MKRLPGILALLILALVPSMAMAHQGNPDYRSEITSVQPEALGEG
                     LTIEIVNFDDHVRLVNKTGKVVLIKGYDGEPYARVEADGKVFVNLNSPAYYLNEDRFA
                     AVEVPKRADAKAAPDWKQIADNGTFEWHDHRSHYMGTGTPSQVTDESKETKVFDYVIP
                     IEVGGEPAKINGTLTWVGTGSKAPVIPFVILGLAIIAAIVFWLMRRRRSEDDDGPREG
                     GSGEAGGSGDDNPDESGKSEAW"
     gene            complement(6083..6397)
                     /locus_tag="PLJ64_08520"
     CDS             complement(6083..6397)
                     /locus_tag="PLJ64_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93565.1"
                     /translation="MEADRNISPPRSAGLLFVAAIWALLLTLVGSPEVVFFTVPVFLL
                     AAPLAFGRYTGEGLIGALRGGRVRTDGRDLPRHLIDAARRAVTPVHFERRLGRAPPVS
                     VN"
     gene            6451..7317
                     /locus_tag="PLJ64_08525"
     CDS             6451..7317
                     /locus_tag="PLJ64_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93566.1"
                     /translation="MAQIKALLFNDPSCPWGYSASPALRTLEWRYRDQIEWHLAVIGL
                     SDENHPVPYPPETLSDLYFDFRCRYGMPFAIETKIRPFTSATSCKAITAARLLYPGSE
                     WRVLRTLQLLHFNTPLLLDDRTQLGEALGTVPGLDASTVMTAIDSPEVAAAYRTDYDH
                     ARSATGSPTHLQAKTASHSTGERYTAPSVIFEYGDKRAEVGGFQPVEAYDVMIANIEP
                     GLKRMGPAEDPLEALGLYPAGLTTQEVAAIMASGMDKPERVDAERKLVHLLGERKVKR
                     VPLGDDALWIKA"
     gene            7370..>7844
                     /locus_tag="PLJ64_08530"
     CDS             7370..>7844
                     /locus_tag="PLJ64_08530"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNC93567.1"
                     /translation="MALGGAEVAARESGSPTAKAAGSNFRPDIQGIRAIAVTLVLACH
                     ASIPWAKGGFIGVDVFYVLSGFLITGLVVREIEKTGKVSIRNFYARRAKRLLPMAALV
                     LVFICIGTLFIFSLARQVEIGGDVFAAALYFVNWHFIIQGVDYFAEVWVNDTCVGR"
BASE COUNT         1401 a         2531 c         2497 g         1315 t
ORIGIN      
        1 ttccttccgc tccgagctgc atcatcaagg cggcgtcggc cggggtggcg atgccgcccg
       61 cgcagaagac gggaaccggc agccagcccc gggtcgccac gtcacggacc agctccagcg
      121 gagcctggag ttccttggcg gcggtcggca gctcggtcga actgagggtg gaaagtcgct
      181 tgatgccgcc gacgatctcg cgcatgtggc ggacggcctc gacgacgttg ccggtgcccg
      241 cctcgccctt cgagcggatc atcgccgccc cctcgccgat ccggcggaga gcctcgccga
      301 ggttggtcgc accgcagacg aagggcgcgt cgaacccgaa cttgtcgacg tggttggtct
      361 cgtcggccgg ggtcaggacc tccgactcgt cgatgaagtc gatcgaaagc gactgcagga
      421 cctgggcctc gacgaaatgg ccgatccgga ccttggccat cacgggaatc gaaaccgcgt
      481 cctggatctc cttgatcatc cgcgggttgg acatgcgggc cacaccgccg tccctgcgaa
      541 tgtcggccgg gacccgctca agggccatga ccgcgcaggc ccccgcgtcc tcggcaattt
      601 tcgcctggtc ggcgtcgacc acgtccatga tcacgccgcc cttcagcatc tctgccaggc
      661 cggtctttac tcggaaagtc gcttcttcag ccatcccctc caatatagag agagaaggcc
      721 ctcgaaggtc cggtcaaacg caggtgatgc cggtggcgtc ctgacgaatc gtctacttca
      781 gcttgtaagc gcggatccgt tcgctgtgct cgtcttcgct cagcgagtag acgccgtagg
      841 ccagcgcgtg aaccagcgcc atcagggcgg tattcgaccg caggaaggac gggctgttgg
      901 atgagaagta aaggctcatg tcggccttct tgaccgcgtc ggaaagggtt gcgtccgcga
      961 tcgcaagggt ccgggcgccg cgatggccgg ccagcttcat ggcccgggcg agcagcgcat
     1021 gcgagcgtcc cgaggaaagg gtgatcagca gcgtttcttc atccacccgc cccaggcgct
     1081 gaagcgaggg gccggcggtc gaggtgacga tctcggcccg gatgtcgagc aggctcaggg
     1141 tgtgacggag gtaagaggcg aagaaggcca tctggtcggt gccgacgatg acgacctggc
     1201 tggccccgga aagggcggcg acnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nntgggagcg ggaaaactcg tcgatccgat cctggatgta agttgacagg gtcttggttt
     1381 cggcggtcac tgggctcacg ccggatcaat tcgccccgcc cgttcaattc ccttctaaat
     1441 tcgctctgag caggactttt gaatcagttc cagggacttc cgaaccgcaa gacctcactc
     1501 accggacaca ccgacgaggg tgacgagatc tggatcatcc gctcgatctc gcagaagttc
     1561 tacaagtgcc tcggctgcaa gggggaaatc aacatcgggg acgagcatgt ggtcgtccag
     1621 tacgtcggga agtacggcgg caccgagcat tcgcactggc atcagcgctg cgccgaggag
     1681 atcctcttct accaggtgcg tggcatgcga aaggtctcct cgaaagagtc aagccgggac
     1741 cggctcgaaa gccggggccg ccggcctgcc ggacgcaggc gacgtccccg ctgaactttc
     1801 tgcaaaacgt cgcgggcgcg ctgaacttca tgcttcccgc ggctggccct tactgcttgc
     1861 ggcgggcccg aaccacgatg ccggcgaccg tgccgatcgc cagcccggcg atgatgatca
     1921 ggatcagggt cttgctgtcc gagccctcgt catcggagga cgagtcctca gcgtcggccg
     1981 cggtcgcggt cggctcaccg gtcgcggcgg cgtcgccttc ggcgacctgg tcatcgtccg
     2041 actgctgatc ggccggagtg gcgggtgaca cctgggcctt cgcggtcgat gtgagggtca
     2101 cggccggggc cgggtactcc ggttcgacgt caccttccat cgcctgaatc caggcggtcg
     2161 aggcgtcgcc ctgacatttc tggatcgtct tgaacatcac gttctcgtcc ttctttccgt
     2221 agagggtcac cgtgaagggg aaatcctgag tctggtgatc gggcaacgga ccgcccgtcc
     2281 aggtcagggt gttgccagcg gaagtcgcct tccagccctt gacgtttccg gccttgacat
     2341 cggcggcggc cttcggaatc tgaacgacga tcgaggtcgt cgccgcgccg tcacagccat
     2401 gggggatctc gaagctcaga tcgacaggct tgccggccgg ggcttcatca ggggtcatct
     2461 cgacatgcgc cgaagccacc ccggcgaaaa ccggggtggc gagcagcaca gccagaagga
     2521 cgaggaaact tccgactttc ttcattggtt cagcctactt gcgacggctg ccgacgaggg
     2581 cgaccgaacc gacgaccaat cccagcgcac cgacgatcaa cgctgcgatc gcgagaccgt
     2641 tgtccgagcc atcgtcgtcc gacgaatcag cggaagcagc ttccgccgaa tcatccgcgg
     2701 cggccatgtg gtcgtggcct tcctcggacg cggcggtcag catcacggtc ggggccgggt
     2761 gttccggttc ctcgccgctg gccggggtcg actggatcca ggcagtttcg gcaccaccct
     2821 cgcagccctg gattaccttg aactcggcgg catcgccctc ggcgcctgca aaggcgacgc
     2881 tgagcgggaa ctgctgcagc tggtcatcgg caagcggacc gccggtccag gtgacttgac
     2941 gaacaccttc ggtgatcttc tcgccgtgca tctcgctcgg ctggggcagc ttgccttcca
     3001 cggtcttgat gttccagccg ggaaccacgc cgggagttgc ggcgaccacc tgcgggggca
     3061 tctggacctt gacgctcgtt gtcgcagcgc cgtcacagcc atgcggcacg gtgaaggtca
     3121 gcatcgcgta gtcgccggcc ggagcttcct ccggggagac ggtcacatgt gccgacgcag
     3181 tggcgggaat tgccaggaca aacagggcgg cgaggcccag gatgattttt ctcttgatgt
     3241 tcatttgatt cctttccggc gggttcgccg gtcaattcat tgcttgagtt ctctggatca
     3301 gatgaaacaa ggcggtgccc ggccggccag cgagcgggcg ataaggcgac caccccggtc
     3361 cggaacagga acgaaaacaa tctgcggctc gacgacgcgg ccgccaaacg agtgcccgag
     3421 ccagtccgag agcgcccggt ggacccgctc gatcgcccgc tcgccgggat accggccgag
     3481 cagcaggagc aagaccagca gcaacagggg cgcgcaagca ggcaggaatc cggtcaacca
     3541 gtgactcatt tcacttccac ctcgaactcg gcgatcggct cgtcgaattc ggagagccgg
     3601 atcgccacct cggccctcca ctccccctgc accccgagca tggccgaggg cacgacgaaa
     3661 tgcccggggc cggctcgcct ggcgtcggct tcgatcggag cgatgtcttc cgcggggagc
     3721 gagaaggaaa cctccatcga ccgcaccggc accggggccc ccgtgcggtc atcgaacacg
     3781 tagaggtgga cctcgttgct tccgacccgg gccggatcga ccgtgtactc gacccgcttg
     3841 ccctggatct cgaccgcgcc cgaggccgga ccggactgga tcgcgtccgg cggcgggtag
     3901 gagaccagag ccgcgctgac tccgagcacg acggtgacga gtatgacttc gatccgcagg
     3961 gagctccgga gccggttacc cggggccccg ggagactccg agcgttcgag ccttcgcacg
     4021 agcccgggga tgatccggtt gcgattgatg aacccgaggc cgatcaggat tccgaacagg
     4081 acgatcttga tcgacaccgc ccgaccgaac tgggtttcga caagatcggg aaccgagccg
     4141 acctcgatga tcgcctgaac cgcacccgag accgagatca tcgatacggc cgccagagct
     4201 accgcggaaa aactccgggt cgcccgggtc aggagtccgg ttcgatcaga cggctcgggc
     4261 agctggcggg tcgcggcggg cagcagggcc agcatggcga ccagcccgcc ggtccagatg
     4321 gccatcgcga ccgcgtgaat gatgtcgctc gggaccagca gccaggccgg gtcccgggtc
     4381 gaggcgtgac cggcgaaggc cggcgaggca gcaagcacca ggccggccag gcaggcgagg
     4441 atcgtgagcg gaccgggccg ggtgtagcgg cccctgccgg ccagaacgag cccgatcaga
     4501 gccagccagg cggcggagcg gatcagcatt accgtgccga atctggtgtg gatcacctcg
     4561 cccggtatcc ctgagccgaa ggcccgccag aaactggtgc cagccccgat cgctccctgg
     4621 aagatgatcg ccatcagcga agccagcagg ccggccagtc ccacaaccag gatcagccgg
     4681 ctgaagcgac gctcgatgac ccggcggtca attcccggtg tgtttgacag cggaccccag
     4741 atcagaagca tccaggcgag ggcaccgacc gcaagggcaa tcgccaggta gccgatccag
     4801 cgatcggccc agaaggcaac ctcggtgacc ttgcccgcct cgctttcggc caggagttcc
     4861 gaaatcgtct tgccctgggt gaaggcggat gcgtccttgc cgggtttgcc gatcgtgaaa
     4921 gtgatgccgc ccgaaaccgg gtgggaatcg gccgagatca catggtaggt cgcggtgtaa
     4981 agcccgtcgg gcaggtccgc gggcaggctg gctccgaccg cgtccgcgtt ttcacccgga
     5041 cgctcgaggt catcggtctg gacctggtca ccggatgtgt tgaagacccg gaccgcaccc
     5101 agcgacgctt cgaccggctc gttgaaggtg aagtccacgt ggccgggtga ggagttgagc
     5161 tccgcccccc gagtcgggga tgaaccctcg agcacggcat gggcagaggc cgaactggcc
     5221 agcccgagca ggcaggccag gaaaagcaga cttgtgagga ggctgcgcct caccaggcct
     5281 cactcttgcc tgattcatcc gggttgtcat cgcccgatcc acctgcttcc ccggatccgc
     5341 cttcgcgagg tccgtcatca tcttcgctgc ggcgtcgccg catcaaccag aagacgatcg
     5401 cggcgatgat cgccagcccg aggatcacga aggggatcac cggcgctttc gatccggtac
     5461 cgacccaggt cagggtgccg ttgatcttcg ccggctcgcc accgacctcg atcgggatca
     5521 catagtcgaa gaccttggtc tccttcgact cgtcggtcac ctgcgagggc gttcccgtgc
     5581 ccatgtaatg cgagcggtgg tcatgccact cgaaggtgcc gttgtcggcg atctgcttcc
     5641 agtcgggagc ggctttcgca tcggcccgct tcggcacttc caccgccgcg aaccggtcct
     5701 cgttgaggta gtaggccggc gaattcaggt tcacgaaaac cttgccgtcg gcctcgaccc
     5761 gggcgtaagg ctcaccgtca tatcccttga tcagaacaac ctttccggtc ttgttgacca
     5821 ggcgaacatg gtcatcgaag ttcacgatct cgatcgtgag tccctcgccc agcgcttccg
     5881 gctggaccga ggtgatctcg gaccggtagt cgggattgcc ctggtgggcc atcgccatcg
     5941 aaggaacgag ggcaagtatg agcaaagcaa ggatgcctgg gaggcgcttc atggtcactc
     6001 cataaccgca gattgcgagt cggtgcggct acttgccgcg gggtcgggtc ggattatgcg
     6061 agccggcccg ataccggccg gctcagttca cggaaacagg tggcgcccgg ccaagccgcc
     6121 gctcgaaatg cactggcgtg accgccctcc gggcggcgtc gatcagatgc cgcggcaggt
     6181 cgcggccgtc ggtccggacc cgacctccgc gcagagcccc gatcagacct tcccctgtgt
     6241 accggccgaa ggcgagcggt gcggcgagca ggaagacggg aaccgtgaag aaaaccactt
     6301 cgggggagcc gaccaaggtc aggagcagcg cccagatcgc ggcaacgaac agcaacccgg
     6361 cgctccgggg aggcgaaata ttccggtccg cttccatcac atgaatccta acccgcgagt
     6421 cgaccaaaag gagtggctag gcttcgcggc atggcgcaga tcaaggcact tctcttcaat
     6481 gacccgtcct gtccctgggg ctactcggcc agtcccgccc tgaggactct tgagtggcgc
     6541 taccgcgacc agatcgagtg gcatctcgca gtgattggcc tttctgacga gaaccatccc
     6601 gtaccctacc cgccggagac tctctccgac ctctacttcg atttccgctg ccgctacggc
     6661 atgccttttg cgatcgagac aaagatccgc cctttcacca gcgccacgtc ctgcaaggcc
     6721 atcaccgccg cccgactgct ctatccgggt tcggaatggc gggtcctccg cacccttcag
     6781 ctgctccact tcaacactcc cctcctgctc gatgaccgga cccagctggg cgaggcactg
     6841 ggaacggtcc ccgggctaga cgcgtcgacg gttatgacgg cgatcgacag cccggaagtg
     6901 gccgccgcct accggaccga ttacgaccac gcccgcagcg cgaccgggag tccgacccac
     6961 ctccaggcca agaccgcttc ccactcgacc ggcgaaaggt atacggcacc ctcggtgatc
     7021 ttcgaatacg gcgacaagcg agccgaagtg ggcggcttcc agccagtgga ggcctacgac
     7081 gtgatgatcg ccaacatcga acccggcctg aaacgcatgg gtccggccga ggatccgctg
     7141 gaggcgctcg gactttaccc ggccggcctg accactcagg aggtcgcagc gatcatggct
     7201 tccgggatgg acaagcccga gcgcgtggac gccgaacgaa agctggttca cctgctcgga
     7261 gagcgaaaag tgaaacgggt tccgctgggc gacgacgccc tctggatcaa ggcttgaggt
     7321 acccgcgctg agcgagcggg gcgtcacccc aaacctgtag cctgcgccga tggctcttgg
     7381 cggtgccgag gtcgccgcca gagaaagcgg ctcccccacg gcaaaagcag cgggttcgaa
     7441 cttccggccc gatatccagg gaatccgcgc gattgcggtc acccttgtcc ttgcatgcca
     7501 cgcttcgatc ccctgggcga agggtggatt catcggggtc gatgtcttct acgttctctc
     7561 aggcttcctg atcaccggcc tggtcgttcg cgagatcgag aagacgggca aggtctcgat
     7621 caggaacttc tatgcccgcc gcgccaagcg cctcttgccg atggccgcgc tggtcctcgt
     7681 cttcatctgc atcggcaccc tgttcatctt ttcgctggcc cgccaggtcg aaatcggcgg
     7741 agatgtattc gccgccgcgc tctacttcgt caactggcat ttcataatcc agggcgtcga
     7801 ttatttcgcc gaagtatggg tgaacgacac ctgcgtcggg cggc
//
LOCUS       DAOQKN010000120         7795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_4549_length_7795_cov_7.265504, whole genome shotgun sequence.
ACCESSION   DAOQKN010000120 DAOQKN010000000
VERSION     DAOQKN010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 7795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7795
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4549_length_7795_cov_7.265504"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="PLJ64_08535"
     CDS             <1..833
                     /locus_tag="PLJ64_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HNC93568.1"
                     /translation="LMDAVPVTLGQEFGGYASQVRHGIDRVRGTLPRVGQIPLGGTAT
                     GTGLNTHPEFSAKVRGLLEAESGLAIAPPHDRFEAQANRDSLVELSGALKVYAVSLNK
                     IANDLALMGSGPRTGLAELHLPELQKGSSIMPGKVNPVIPEVVIQVAAQVVGNDMAIT
                     MAGSQGQFELNVRVPLIARNLLGSIRILASATRLLDEKCVTGIEANHPQHEKHAEATL
                     ATATALNPYIGYDKASEIVKEAAASGRPLREVAIEKGVEEKVLDEALDHHKMANPHSA
                     "
     gene            1106..1423
                     /locus_tag="PLJ64_08540"
     CDS             1106..1423
                     /locus_tag="PLJ64_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93569.1"
                     /translation="MSVISHVSRESKATAVVLLLAWLAVAALSVTVALIAAPAFALFF
                     VLSLGFFPGEQLIVKARELVSRPREWSRPVPIAAPVLPFSSRPVGLDLAFALAVRPPP
                     VLQ"
     gene            1509..2513
                     /locus_tag="PLJ64_08545"
     CDS             1509..2513
                     /locus_tag="PLJ64_08545"
                     /inference="COORDINATES: protein motif:HMM:TIGR04052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallo-mystery pair system four-Cys motif
                     protein"
                     /protein_id="HNC93570.1"
                     /translation="MTIAHFRCVIVDEQLENNMKSSKKLGAIFAALALGLVGLIALPV
                     TANAHGGHAKKSKTQKVAIGFKAVNGNTPVSCSKPITGLGTTSRTAKLTDLRFYVSGV
                     QLLRKGGGAVRVKLQGGSKWSYTKGKDAVTLIDLENGAGACSAEGTKATNAFVRGTVP
                     KGRYVGVRYSVSVPDSLSHTDLTAMPSPLNNTAMGWSWQYGRKFMKIELGQGDTPDWA
                     SPIYYLHLGSTNCTGDPAAGEQAKCDLPNQNLITFRKFNPAGQRIAIDFKKLFSGVNV
                     AGESMDMGDGMDMGGMMAMGGCMSATDSPDCDPLFETLGLKLGSTKRTNQTAFRVVRK
                     "
     gene            2516..3733
                     /locus_tag="PLJ64_08550"
     CDS             2516..3733
                     /locus_tag="PLJ64_08550"
                     /inference="COORDINATES: protein motif:HMM:TIGR04039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme enzyme"
                     /protein_id="HNC93571.1"
                     /translation="MGRDRNKSNGRFAVPRRFTTRAMALAAIFLAVSAAAFGLQATGA
                     LKAPNNDWSWALPRNFPTPRVPARNPMSQGKVNIGHRLFYDVRLSGNQTQSCSSCHLP
                     ELAFTDGKTTSVGSTGDVLLRNAPSVANSAYHRTFTWANPALTSLESQMNVPLFGTGP
                     VEMGVNDRNKGKILRRIKSDPWYRKRFPKAFRWSKKPVNWFNIVRSISAFQRSIITGT
                     SKYNRFLRGKAELSGSERSGLNLFMGEEGECHHCHGSFIFDDQATYAGGPLEKLKFHN
                     TGLYNIGGTGAYPTGNRGLYEITGKKKDMGAFRAPSLLNVAKTAPYMHDGSVKTLMDV
                     VDIYARGGRNITEGPFAGDGATNPHKDPLISGINLSEQDKKDIVAFLKTLSERDYSKD
                     PRFWDPFKHKSRK"
     gene            3736..4101
                     /locus_tag="PLJ64_08555"
     CDS             3736..4101
                     /locus_tag="PLJ64_08555"
                     /inference="COORDINATES: protein motif:HMM:NF016149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HNC93572.1"
                     /translation="MDSITSSNTVRGLVAGTIVAAAMILGGPAGQAEAHTGVTSTSAN
                     SRMLRVFFAAPIRRGTLRARNASGAIVSKGRGGRDPRNINALRVGLRNLRGGYYRATW
                     SIVGLDGHHQSGTVRFRVR"
     gene            4106..4750
                     /locus_tag="PLJ64_08560"
     CDS             4106..4750
                     /locus_tag="PLJ64_08560"
                     /inference="COORDINATES: protein motif:HMM:NF019599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1775 domain-containing protein"
                     /protein_id="HNC93573.1"
                     /translation="MSRRVSSFIACALIGGSALVLAVPAAAHIQVEPTEVAPGDAALF
                     TLLVPGESAAGTSEVKLKVPPGVYPFSYEETPGWKRKLVKKPNGMVDQVIWTGTAAPD
                     GLVRFSFLAGTPEKLGEIKWAAIQNYANGEESRWIGMPESENPAPVTIVSDSVPRQNA
                     GGESEVAHEHASPTSSENATTDSETDWPITIAALLGFFFGLSSLVILLVNRINS"
     gene            complement(4747..6993)
                     /locus_tag="PLJ64_08565"
     CDS             complement(4747..6993)
                     /locus_tag="PLJ64_08565"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNC93574.1"
                     /translation="MNRFRTKVAAALVAALGLLGVAVAQAATGDTLGVTTVTQRIVPD
                     SSAGFNFLAAGPGEAYTVRDGSEEGGVSLGSADAGRATRRTSISYFGQLTDFQLADEE
                     SPLRVEFLDPQGGAFTSAWRPGEAMNPQEEDAMMRQFNAFASKPPNLAKSGAKPKMDF
                     VVNTGDISDNNQYNEALWNLQIAEGGTVNPGTGVDPDPYVGEHPLCPAGMNVLNADDP
                     TAYTGVQDRDEWPAPTMGYFWDPDQPDPGPVAVNPFADWPSYPGLMDRSQRPFKATGL
                     KVPSYFVFGNHDNLVQGNAWGSGIFNQIATGCLKPINDDAANSGRSSNPLLSFVLNPN
                     FTAQDFLDIRTGEPKYFMEVPPDPDRHLISKKEAMNLYRSTGKSNGHGFGFVDQASRT
                     ASNGYASYYSFSPRPGIRYISLDTSSEGGTVLVSDKGNLDDPQFKWLEAQIKKATANN
                     ELVVLFSHHAAGSMTANVPDEAAASCTQVDVAVAPGCDGDPRDSSPIHLGTDVVTMLH
                     NYPNVIAWVAGHSHVNDVTPFPAPDGKSGFWSIRTAALADWPKQNRLVEIFDNRDGNL
                     SIFGTVIDHAAGVDAPASGTSAANMSTDELASISRVVGYNENQSGAEACGSEKCGEGE
                     AEDRNVELVIKDPRKPKAIVKKVTVAPKKRNLKAGRKFNLVVKVTNFITATADAKNVK
                     VTVKSSNKRVKVKSAVIRKISPGKTATVKIPVRALGKARGKSKITVTAAGNKAYSNIK
                     VIPKKKRR"
     gene            complement(7199..>7795)
                     /locus_tag="PLJ64_08570"
     CDS             complement(7199..>7795)
                     /locus_tag="PLJ64_08570"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNC93575.1"
                     /translation="GQSCISIQRILVHEAVADRFIERLVANVEKLKVGDPSLDDTDVG
                     PLITPGDRDRVKEWIDEAAAGGAKVLTGGELVDEGRCLAPTLIEGPSREAKVWCEEIF
                     GPVATVDRFSDFDEALARANDSKFGLQAGVFTRDVGNGLKAGNTLEFGGVLINEVPTF
                     RADQQPYGGVKDSGNTREGPAYSIIEMTEERFVTFQAG"
BASE COUNT         1421 a         2471 c         2483 g         1420 t
ORIGIN      
        1 acctcatgga cgcggttccg gtcaccctcg gccaggagtt tggcggctac gcctctcagg
       61 tccgtcacgg catcgaccgg gtccggggca ccctgccccg ggtcggccag atcccgctcg
      121 gcggcaccgc gaccggcacc ggtctgaaca cccaccccga attttccgcc aaggtccgcg
      181 gcctgctcga ggccgagagc ggtctcgcga tcgccccgcc gcatgaccgc ttcgaagccc
      241 aggccaaccg cgactccctg gtcgagcttt ccggcgcgct caaggtctac gcggtttcgc
      301 tcaacaagat cgccaacgac ctcgccctga tgggctccgg cccgcgcacc ggcctggccg
      361 agcttcacct gcccgagctg cagaagggat cttcgatcat gcccggcaag gtcaacccgg
      421 taattcccga ggtggtgatc caggtcgccg cccaggtggt cggcaatgac atggcgatca
      481 ccatggccgg ctcccagggc cagttcgagc tgaacgtgcg ggtgccgctg atcgcccgca
      541 acctgctcgg ctcgatccgc atcctcgcca gcgccacccg cctgctcgac gagaagtgcg
      601 tcaccggaat cgaggccaat cacccgcaac acgaaaagca cgccgaggcc accctcgcca
      661 cggccaccgc actcaacccc tacatcggct acgacaaggc cagcgagatc gtcaaggaag
      721 cggccgcttc aggacgaccg ctccgcgagg tggcgatcga aaaaggggtc gaagaaaagg
      781 ttctcgacga agccctcgat caccacaaaa tggccaaccc ccattcggcc tagtcgcaac
      841 cggttttgtc gaagggcccg gcgcgggggt ggtccacgtt gaacggaggt gccgcacgtt
      901 ttgcagcgtg atgctgctgg atgtgcggca cctctgaagt tcgaccgggt tgatgtagcc
      961 gtggggtcgg gtgagcgtcg ccgggacgct caatgacagc gcggctgaat gccgcgcgtg
     1021 tcgtgtcccg gcgatgctca cccgacccca cggcgtaaca ggcgggaacg aaactttttg
     1081 gctcccggta ctgtttgagt agctgatgag tgtgatttct cacgtttcgc gtgaaagcaa
     1141 ggcgaccgct gtcgtcctgc tgctcgcgtg gctggcagtg gcagcgcttt cggtgaccgt
     1201 cgccctgatc gccgctccag ccttcgcgct cttcttcgtt ttgagcctcg ggttcttccc
     1261 cggggaacag ttgatcgtca aggcgaggga actggtttcg aggccgaggg aatggtcccg
     1321 gcccgttccg atcgcggcgc ccgtgctgcc tttttcttcc aggccggttg gcctggacct
     1381 ggctttcgct ctggcggtca gaccgccgcc ggtacttcag tagtgactga gggcctcttc
     1441 acctgaaggt gaaggaggca tcaatcaagc gggaactcgc tgcggcttga tcgtcacagg
     1501 cgggtcccgt gacgatcgcg cacttccggt gcgtgatcgt cgacgaacaa ctggagaaca
     1561 acatgaagag cagcaagaaa cttggcgcga ttttcgccgc cctggccctt ggcttggtcg
     1621 gcctaatcgc cctccccgtc acggcaaacg cccatggcgg acatgcaaag aagagcaaaa
     1681 cccaaaaagt ggcgatcggc ttcaaggcag tgaacggcaa cacgccggtc agttgcagca
     1741 agccgatcac cggcctggga acgacctccc ggaccgcaaa gctgactgac cttcgtttct
     1801 acgtctcggg cgtacagctt ctgcgcaaag gcggtggagc cgtcagggtc aagttgcagg
     1861 gtggttcaaa gtggagctac accaagggca aagacgcggt gaccctgatc gacctcgaga
     1921 acggtgctgg cgcctgttcg gccgagggga ccaaggcgac caacgcgttt gtccggggaa
     1981 cggttcccaa gggtaggtac gttggcgtcc gctacagcgt cagcgttccc gattcactca
     2041 gccacactga tctgaccgcg atgccgtcac cgctgaacaa cacagcgatg ggctggtcgt
     2101 ggcagtacgg ccgcaagttc atgaagatcg aactcggtca gggtgacacc ccggattggg
     2161 cttcaccgat ctactacctg cacctcggca gcaccaactg caccggggac ccggcagcgg
     2221 gcgagcaggc gaagtgtgac ctgccgaacc agaacctgat caccttcagg aagttcaatc
     2281 cggccggcca gaggatcgcc attgacttca agaagctgtt cagtggagtc aacgtcgccg
     2341 gtgagtccat ggacatgggc gacggcatgg acatgggcgg catgatggcg atgggcggct
     2401 gcatgtcggc caccgactcc cccgattgcg atccgctctt cgagacgctc gggttgaagc
     2461 tcggttcgac caagaggacg aaccagaccg cgttccgagt cgtccggaag tagtcgtggg
     2521 gcgggaccgc aacaagagca acggccgctt tgcggttccg cgccggttca cgacccgggc
     2581 catggcgctg gcggcgatat tcctcgccgt cagcgccgcg gccttcggtc ttcaggccac
     2641 tggcgccttg aaggcgccca acaatgactg gagctgggct ctgccccgga acttccccac
     2701 gccccgggtg ccagccagaa accccatgag ccaggggaag gtgaacatcg gtcaccgcct
     2761 cttctacgac gtccgcctgt cgggcaacca gacccagtcc tgctcttcct gccaccttcc
     2821 cgaacttgca ttcaccgacg gcaagacgac ttcggtcgga tcgaccgggg acgtcctcct
     2881 ccgcaacgct ccttcggtcg ccaactcggc ctaccaccgg acctttacct gggccaaccc
     2941 ggcgctcacc tcgctcgaga gtcagatgaa cgttccgctc ttcgggaccg gcccggtcga
     3001 aatgggcgta aacgaccgga acaagggcaa gatccttcga aggatcaaga gtgatccgtg
     3061 gtaccgaaag cgcttcccga aggcattccg ctggtcgaag aaacctgtca actggttcaa
     3121 catcgtccgc tcgatcagcg cattccagcg cagcatcatc accgggacct cgaaatacaa
     3181 ccggttcctg cgcggcaaag ccgaactcag cggttcggaa cgaagcggcc tcaatctctt
     3241 catgggcgaa gagggtgaat gtcaccactg tcacggcagc ttcatcttcg atgaccaggc
     3301 gacctacgcg ggcgggccgc tcgagaagct caagttccac aacaccggcc tctacaacat
     3361 cggtggaacc ggcgcctacc ccaccggcaa ccgcggcctc tacgaaatca ccggcaagaa
     3421 gaaggacatg ggtgctttca gggcaccgtc gctcctcaac gtggcgaaaa cggcccctta
     3481 catgcatgac ggctcggtca agacgctcat ggatgtggtc gacatctatg cccgcggtgg
     3541 ccgcaacatc accgaaggcc cattcgccgg tgacggggcg acgaatcctc acaaggaccc
     3601 gttgatctcc ggaatcaacc tcagtgaaca ggacaagaag gacatcgtcg ccttcctcaa
     3661 gacgttgagc gaacgcgatt actccaagga cccccgcttc tgggatccct tcaagcacaa
     3721 atcaaggaaa tgaacatgga cagcatcacc agcagcaaca cggtccgcgg tctggtcgcc
     3781 ggcacaatcg tcgcggcagc aatgatcctc ggtgggccgg ccggccaggc ggaggcccac
     3841 accggtgtca catcgacctc cgccaacagc aggatgctcc gggttttctt cgctgccccg
     3901 atcaggaggg gaaccctgag agcccgaaac gcgagcgggg cgatcgtgtc caaaggccgc
     3961 ggaggacgtg acccacgcaa catcaacgcg ctgagggtgg gactgcggaa tctgcgtggc
     4021 ggctattaca gggcgacctg gagcatcgtc gggctcgacg ggcaccacca gtcgggaacc
     4081 gtgcgcttcc gggtgaggta gccccttgtc aaggcgggtc agcagtttca ttgcctgcgc
     4141 cctgatcggc ggatcagccc tggtcctagc cgttcccgcg gcagcacaca ttcaggttga
     4201 acccacggag gttgctccgg gagacgcggc actcttcacc ctgctggttc ccggcgaatc
     4261 tgccgctgga acttccgaag tcaagttgaa ggtccctccg ggcgtttacc cgttttccta
     4321 cgaggagact cccgggtgga agcggaaact cgtcaagaaa ccgaacggga tggtggacca
     4381 ggtgatctgg accggaacgg cagccccgga cgggctggtg cgtttcagct tcctggccgg
     4441 aacgccggag aagctgggtg agatcaaatg ggcggcaatt cagaactacg cgaacgggga
     4501 ggagtcacga tggatcggaa tgccggaatc ggaaaatccc gccccggtca cgatcgtttc
     4561 cgattcagtt ccccgtcaaa acgccggcgg cgaaagcgag gtcgctcacg aacacgcttc
     4621 gccgaccagc tccgaaaacg cgaccacgga ttcggagacg gactggccta tcaccattgc
     4681 cgcattgctc gggttcttct tcgggctctc gtcgctggtg atcctgctgg tcaacagaat
     4741 aaactcctag cgacgcttct ttttggggat caccttgatg ttcgagtaag ccttgttgcc
     4801 ggcggcggtt accgtgatct ttgactttcc gcgggccttg ccgagggcgc ggaccgggat
     4861 cttcacggtg gcggtcttgc cagggctgat cttcctgatc accgcggact tcaccttgac
     4921 ccgcttgttc gatgacttga ccgtcacctt gacgttcttc gcgtccgcgg tggcggtgat
     4981 gaagttggtc accttgacca ccaggttgaa cttgcgccct gctttcaggt tccgcttctt
     5041 cggggccacg gtgacctttt ttacgatcgc cttcggcttg cgcgggtcct tgatcaccag
     5101 ttcgacgttg cggtcctcgg cttcgccttc gccgcacttc tccgaaccgc aggcttccgc
     5161 gcccgactgg ttctcgttgt aaccgaccac ccgggaaatc gaggcgagtt cgtcggtgct
     5221 catgttggcg gcgctggtac cgctcgccgg tgcgtcaacg ccggcggcgt gatcgatcac
     5281 ggtgccgaag atcgagaggt tgccgtcacg gttgtcgaag atctcgacca gacggttctg
     5341 cttcggccag tcggccaggg ccgcggtgcg gatgctccag aaaccgctct tgccatccgg
     5401 ggccgggaag ggcgtcacgt cattgacgtg gctgtggccg gccacccagg caatcacgtt
     5461 cgggtagttg tggagcatgg tgaccacgtc ggtcccgagg tggatcggcg aggaatcgcg
     5521 cgggtcacca tcacaacccg gcgccaccgc gacgtcgacc tgggtgcagg aagccgccgc
     5581 ctcgtcgggg acgttggcgg tcatgctgcc ggcggcgtga tgcgagaaga gcaccaccag
     5641 ttcgttgttg gcggtcgcct tcttgatctg ggcttcgagc cacttgaact gcgggtcatc
     5701 gaggttgccc ttgtccgaga ccagcaccgt gccgccctcg gatgaggtgt cgagcgagat
     5761 gtagcggatt ccgggacggg gactgaacga gtagtaggag gcgtagccgt tcgaggccgt
     5821 gcgggaagcc tggtcgacga aaccgaaacc gtgaccgttc gacttgccgg tgctccggta
     5881 gaggttcatc gcttccttct tcgagatcag atggcggtcc ggatccggcg ggacctccat
     5941 gaagtacttg ggttcaccag tccggatgtc gaggaaatcc tgggcggtga agttcgggtt
     6001 cagcacgaag ctgagcagcg ggttcgacga acggccgctg ttggcggcat cgtcgttgat
     6061 cggcttcagg cagccggtcg cgatctggtt gaagatgccg ctgccccagg cgttgccctg
     6121 gaccaggttg tcgtgattgc cgaagacgaa gtaggagggg accttcagtc cggtcgcctt
     6181 gaagggacgc tgggagcggt ccatcaggcc cgggtaggaa ggccagtcgg cgaaggggtt
     6241 gacggcaacc gggcccggat cgggctggtc cggatcccag aagtaaccca tggtcggggc
     6301 gggccactcg tcgcggtcct gcacgccggt gtaggcggtg gggtcgtcgg cgttcagcac
     6361 gttcatgccg gcggggcaga gcggatgctc gccgacgtac gggtccggat caaccccggt
     6421 gcccgggttc acggtgccgc cctcggcgat ctgcaggttc cagagcgcct cgttgtactg
     6481 gttgttgtcg gagatgtcac cggtgttgac gacgaagtcc atcttcggct tggcgccgct
     6541 ctttgcgagg ttcggcggct tggacgcgaa tgcgttgaac tggcgcatca tcgcgtcttc
     6601 ctcctggggg ttcatcgcct cgccgggccg ccaggccgag gtgaaggcgc ctccctgcgg
     6661 gtcgaggaac tcgacccgca gcggactctc ctcgtcggcg agctggaagt cggtgagctg
     6721 cccgaagtag gagatcgagg tgcggcgggt ggcgcggccg gcgtcggcgc tgccgagcga
     6781 tacaccgccc tcttcggagc cgtcgcggac cgtgtacgcc tcgccgggac cggcagccag
     6841 gaagttgaag ccggcggacg agtcggggac gatccgctgg gtcacggttg tcacaccgag
     6901 cgtgtcgcct gtcgctgcct gtgcgactgc aactccgagc aagccgaggg cagccacaag
     6961 agcggcagca actttggtcc tgaaccggtt catcaattct ccttgcggcc ggttccccga
     7021 accggcacat gtggtgtggg gctactcgtc cggtctcctc cggacgggga cggaatgagc
     7081 ctacgttacc gccatgtgaa gaaaccggcc aggagcccgg atgttgcgct cacgccggtt
     7141 cgcttctggg ttgaagttat tcgcacatgg gacgaataac ttcaacccag tcgcattctc
     7201 agccggcctg gaaggtgacg aagcgttcct cggtcatctc gatgatcgag taggcgggac
     7261 cctcgcgggt gttgccggaa tccttgacgc cgccataagg ctgctggtcg gcgcgaaagg
     7321 tcggcacctc gttgattagg acgccgccga actcgagggt gttgccggcc ttcagtccgt
     7381 tgccgacatc acgggtgaag acaccggcct gcaggccgaa cttcgagtca ttggcccggg
     7441 ccagcgcctc gtcgaaatcg ctgaagcgat cgaccgtggc caccggaccg aagatctcct
     7501 cacaccagac cttggcctcg cgggaagggc cctcgatcag ggtcggggcc aggcagcgcc
     7561 cctcgtcgac aagctcgccg ccggtcagga ccttcgcccc gccggccgcc gcctcgtcga
     7621 tccattcctt cacccggtcc cggtcaccgg gggtgatcag tgggcccacg tcggtgtcgt
     7681 ccagcgaggg atcacccacc ttgagcttct cgacgttggc gaccagccgc tcgatgaacc
     7741 ggtcggccac cgcttcgtgg acgaggatcc gctggatcga aatgcaggac tggcc
//
LOCUS       DAOQKN010000121         7764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_4574_length_7764_cov_5.419510, whole genome shotgun sequence.
ACCESSION   DAOQKN010000121 DAOQKN010000000
VERSION     DAOQKN010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 7764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7764
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4574_length_7764_cov_5.419510"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..845)
                     /locus_tag="PLJ64_08575"
     CDS             complement(69..845)
                     /locus_tag="PLJ64_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HNC93576.1"
                     /translation="MSDATRSSQGGSAVLEIQGVTKAFGERQVLGGIDLTLREHEAIA
                     LIGASGSGKSTLLRCIDLLVEIDDGDIFLDGEVITDPSVDPVSVRRKLGFVFQAFNLF
                     PHMKVIDNVTLGSVRAQGSGKKEAREEGRALLERFGLGGREEDMPDELSGGQQQRVAL
                     CRALAGNPRALLLDEVTSALDPELVGDVLDAVRDLKQEGMTMILATHEMSFAREVADE
                     VAFLHEGRIIEQGSPGQILSEPNRPETRKFLRRLLAAGRL"
     gene            complement(842..1513)
                     /locus_tag="PLJ64_08580"
     CDS             complement(842..1513)
                     /locus_tag="PLJ64_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HNC93577.1"
                     /translation="MFFSAESFRDSFPDILKAFWIDIKLFMVVEAIVLVLGLLIAVVR
                     TSKASALFPIRMLAAVYCDFFRGIPVILLVYLVGFGIPALGLAGVPTDPLILAGIALS
                     LAYSSYVAEVYRAGIASIHPSQNDAGLATGLTRIQTLRFVIVPQAVRRVRPPLLNDFI
                     SLQKDVALVSVLGIAEAFRTAQIITLSTFNYTPLLAAALLYLAVTFPMARILDHITAR
                     EQINS"
     gene            complement(1700..2581)
                     /locus_tag="PLJ64_08585"
     CDS             complement(1700..2581)
                     /locus_tag="PLJ64_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNC93578.1"
                     /translation="MSRKRLVLFLFAIFALAVFVGCGSSDDDSSSENSGTDSGSTVAA
                     SDCTPANLSTFKDGVLTIGTDSPAYPPYFEDDDPTNGKGFESALGYAIAGELGFNDSQ
                     VEWATVPFNASYAPGPKDFDFDLNQISITPAREKAVTFSEPYYTANQAVLVKKGSDLD
                     GITSLDQLKDASIGVQIGTTSYDAVVEQIDPSSDPKTFDNSNDVVTAFKQDQVDAIVV
                     DLPTAIYLRDAELSDTVVAGQFEQEGGDQWGALMAKDSDLKGCVDQAINTLKEDGTLE
                     EITNQWMADSAKAPLLN"
     gene            2654..3073
                     /locus_tag="PLJ64_08590"
     CDS             2654..3073
                     /locus_tag="PLJ64_08590"
                     /inference="COORDINATES: protein motif:HMM:NF013490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNC93579.1"
                     /translation="MSTEPKSQAVRDEEEGRPCVEMSEGVARYLMALFDLEQEGGPPT
                     QARLARELDVSQPSALQMVRRIRAMGLINEGDLGLTPAGTSSALVLTSRRTAATDLTS
                     EVLGLDPAEARVEAGHLASSATPAFGRKLVAWRKSRT"
     gene            3084..3893
                     /locus_tag="PLJ64_08595"
     CDS             3084..3893
                     /locus_tag="PLJ64_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93580.1"
                     /translation="MTVQDLVEFSLFVGLVVLLILLLASLVRRRPGGLRGTTLKEKLK
                     RFGRFALVRLPIAVGFAVAVFVLGAAGLTLNSHRPPADHEPVVSVPSGSRNVVVSLRL
                     DDCGEPIEGEVRFSSPRSRIGSPVRFYSDAGGWQRLGLTGARARVRRASFSEEEATRK
                     RSLLSCYLQLPILKGAADGYEVHLRLSDQMEVEKDSSVPSPGAYASGAWVWKCGPDET
                     CPAFAAVSYSIEDGTKQVIILVLASVFGALIALLVGEVLIEWARKRFREPD"
     gene            complement(3899..6961)
                     /locus_tag="PLJ64_08600"
     CDS             complement(3899..6961)
                     /locus_tag="PLJ64_08600"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNC93581.1"
                     /translation="MSNPDEREDRNIPDPVELKLYRGRIAEAASRAALEKEPDPAGIW
                     AEAAGPGSYTWLWTTGIKRAPTVLGWAALVICLLATLAGFVDIPLFTGELLAAPQMPV
                     FSSVTLGMVAFALLAMRPGAAESAVQAGVALCYFTIAVCMALLALRMVGIDPDVWGSD
                     ALRSPSVAALVNNPALALCLLTIDRDPPRYRWQRALIPLVGIIFLISVLTKGYQAALD
                     ANVQTDISILGSATVILIYVGYVFLRPDRGLAGFLSSTGPGPSMARLLVPTAVVIPLT
                     MVMVDLISEGLESGESAFIKGLDRLLMLVLLIWMITYASIRLQRFYDRWKQANSELAS
                     QASVLTRMSEGVVVLRIADSRIVLTNPQFDEMHGFDRGELIGRNIDVIAPDDLSEDEV
                     QMRVDVLHELADKGETSYENRSVRKDGTDIWSRVNGVITTVPEHGPVLILVKYDVTGE
                     RIAKSAGRDAEMRFRQVFEQSPIGLCLVTPEGRFEKVNRSFESITGYSAGELSEMTFA
                     DITHPDDLEEDVAMTGELFAGERDGFSMEKRYIRKNGEVVWIYLTVTMLRDQDGKPNQ
                     ALSMVEDITERQRLSQQLKYLADHDPLTGLYNRRRFEEELSSAIDSGHGRGIAILVID
                     LDNFKFVNDSFGHTIGDRLIVRTAELLKSRLRSDDTLARQGGDEYVVVLRDISPEDAL
                     TTAEELVRLIARDVRIVGAEHSARVTASIGVAVSSEISPVPEETLMMQADIAMYEAKD
                     AGRNGARVFQPGEDSHVTLGIDWAGKLRSALENEELLVYAQPIHRLNGEGPPHHELFV
                     RMRDRSGSIIMPGAFLPTAERHDLVQELDSWVISKAISTLAELEGDGCPRLQINLSGR
                     TVGDPKLPEFVQGELSRAGVDPSNLIFEVTETSAIGNMTGAQEFSARMNELGCGFALD
                     DFGTGFASFYYLKHLAFDYVKIDGEFVQNLASDPVNRLLVKALVEISKGMGKRTVAEQ
                     VEDARTLEVLREFGVDFVQGHYLGRPRPLDRSELASLPALPLDTALPG"
     gene            complement(7047..>7764)
                     /locus_tag="PLJ64_08605"
     CDS             complement(7047..>7764)
                     /locus_tag="PLJ64_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC93582.1"
                     /translation="DGTVLDAAARETGLTGQKVRDLLGAFLFSGEDANKQLSDISGGE
                     QRRLSLAILVASGANLLLLDEPTNHLDIESREALEDALLGFPGAVVLISHDRALLEAV
                     GSRTLVCENGELTNHMTGWAEYQKRRDEAAEAEQEKAAASRPAGKSGNARRGGSPGKE
                     VARTRRRVENLEKKIEQAEARLAEIEDELSDPAAWSTPEKSEKATRRHDRAKEKVASL
                     YAEWEEADQVARETAEAATA"
BASE COUNT         1330 a         2496 c         2579 g         1359 t
ORIGIN      
        1 cacctgctcg ctgaccggag cagtaatcgg gaagccactt tgggagggcc acccggcccc
       61 acggccaact agaggcggcc agcggcgagg aggcggcgga ggaacttgcg ggtttcgggg
      121 cgattcggtt ccgagaggat ctgcccgggg cttccctgtt cgatgatccg gccttcgtgg
      181 aggaaggcga cctcgtcggc cacctcgcgg gcgaagctca tttcgtgggt ggcaaggatc
      241 atcgtcatcc cttcctgctt cagatcccgg acggcatcga gcacgtcgcc gaccagttcg
      301 gggtcgaggg cgctggtcac ctcgtccagg aggagggccc ggggattgcc ggccagggcc
      361 cggcagaggg cgacccgctg ctgctggcca ccggaaagct cgtccggcat gtcctcttcg
      421 cggccgccga gcccgaaacg ctccagcagg gcccttccct cctcgcgggc ttccttcttc
      481 cccgatccct gggcccggac cgagccaagc gtgacgttgt cgatcacctt catgtgcggg
      541 aagaggttga aagcctggaa gacgaagccg agcttgcgcc ggaccgagac cggatcgacc
      601 gaggggtcgg tgatcacctc gccgtcgagg aagatgtcgc cgtcatcgat ctcgaccagc
      661 aggtcgatgc agcgcagcag ggttgacttg ccggagccgg aagcgccgat cagggcgatc
      721 gcttcatgct cccgcagggt gaggtcgatg ccgccgagta cctgacgctc gccgaaggcc
      781 ttggtcacac cctggatttc caggaccgct gaacctccct ggctggaacg cgtggcgtcg
      841 ctcacgagtt gatttgctcc cgggcggtga tgtgatcaag gatccgcgcc atcgggaagg
      901 taaccgccag gtagagcagg gcggcggcca gcagcggggt gtaattgaag gtcgaaagcg
      961 tgatgatctg ggcggtcctg aacgcctcgg caattccgag cacggaaacc agtgcaacgt
     1021 ccttctggag cgagatgaag tcgttgagga gcggcggccg cacccgacgc accgcctggg
     1081 gcacgatcac gaaacgcagc gtctggatcc gggtcagccc ggtggccaga ccggcgtcat
     1141 tctggctggg gtggatcgag gcgatgccgg cccggtagac ctccgccaca tatgagctgt
     1201 aggcgagcga aagggcgatg ccggcgagga tcaggggatc ggtaggcacc ccggccagcc
     1261 cgagggcggg gatgccgaag ccgaccaggt aaacgagcag gatgaccggt atcccgcgga
     1321 agaagtcaca gtagaccgcc gccagcatcc ggatcgggaa gagggccgat gccttgctgg
     1381 tcctgaccac cgcaatcagc aatccgagga ccaggacgat ggcctcgacg accatgaaca
     1441 gcttgatgtc gatccagaag gctttgagga tgtccggaaa cgagtccctg aaggactcgg
     1501 ccgagaagaa catctcccgc acgtccggcc agccgctgct ggtcaggatg acggcggcaa
     1561 ggccaccgag aaccaggacc gatgaaagaa cggcgatcgc ctggccgcgc cgcgccctgg
     1621 cctttcgggc cttgtcgcgg gcggcccggc gatcgccggt accgctttga actggagctt
     1681 ccgtggtcag acccggctct tagttgagca gcggcgcctt ggccgaatcg gccatccact
     1741 ggttggtgat ctcttccagc gtgccgtctt ccttcagcgt gttgatcgcc tggtccacgc
     1801 aacccttgag gtcggagtcc ttggccatca gcgcgcccca ctggtcgcca ccctcctgct
     1861 cgaactgacc ggcaacgacg gtgtcggaga gctcggcgtc acggaggtag atcgcggtcg
     1921 gaaggtcgac cacgatcgcg tcgacctgat cctgcttgaa ggccgtaacg acgtcattcg
     1981 agttgtcgaa ggtcttcggg tcggatgagg gatcgatctg ctcgaccacg gcgtcgtagc
     2041 tggtggtacc gatctggacc ccgatcgaag cgtccttgag ctggtcgagg ctggtgatcc
     2101 cgtcgagatc agagcccttc ttgaccagca ccgcctggtt ggcggtgtag tacggctccg
     2161 agaaggtgac cgccttctcg cgggcgggag tgatcgagat ctggttcagg tcgaagtcaa
     2221 agtccttcgg tccgggcgcg taggaagcgt tgaacggcac ggtcgcccat tcgacctggg
     2281 agtcgttgaa tccgagctcg ccggcgatcg cgtaaccgag ggcgctctcg aagcccttgc
     2341 cgttggtcgg gtcgtcgtcc tcgaagtacg gcgggtaggc cggggagtcg gtgccgatgg
     2401 tcagcacacc gtccttgaag gtgctgaggt tcgccggagt gcagtccgaa gcggcgacgg
     2461 tgctgccact gtcggtgccg gaattttcgc tggacgagtc atcgtcactg gagccgcagc
     2521 cgacgaaaac ggccagggcg aagatggcaa acaggaaaag gacaagacgt ttacgactca
     2581 cgagtattcc tccgcaggtt gagcgactga cgtggggagc ctagagcatg ttgagggtag
     2641 aatcgctcgc ggtttgagca cagagccgaa aagtcaggcc gtacgcgacg aggaggaagg
     2701 ccgtccctgc gttgagatga gcgaaggcgt ggcccgttac ctgatggcgc tgttcgacct
     2761 cgagcaggaa ggcggccccc cgacccaggc caggctggcc cgggaacttg acgtttccca
     2821 gccctctgcg cttcagatgg tccgccggat ccgggcgatg ggcctgatca acgaggggga
     2881 cctcggactc acgccggccg gcaccagctc cgctctggtg ctcacttcca ggcggaccgc
     2941 cgcaaccgac ctgaccagcg aggtgctggg actcgacccg gccgaagcgc gggttgaagc
     3001 cggacatctc gcctccagcg ccaccccggc cttcggccgc aagctggtcg cctggcgcaa
     3061 gtcccggacc tgagcccgtt cagttgacgg tccaggacct cgtcgagttc tcgctcttcg
     3121 tcggactggt cgttctgctg atcctcctgc tcgcgagcct ggtccgccgc cgccccggcg
     3181 ggttgcgggg aaccaccctg aaagagaagc tgaaacgctt cggtcgattc gctctggtgc
     3241 gcctgccgat cgcggttgga ttcgccgtgg cggttttcgt gctgggggcg gccggcctca
     3301 cgctgaacag ccaccggccg ccggccgacc acgagccggt cgtctcggtt ccttcggggt
     3361 cgcggaacgt ggtggtttcc ctccggctgg atgactgcgg tgagccgatc gagggcgaag
     3421 tccgcttcag ctcgccccga agcaggatcg ggtccccggt caggttctac tccgatgccg
     3481 gtggctggca gagactcggc ctcaccggcg ctcgggcccg ggtccggcgc gcaagcttca
     3541 gcgaagagga agcgacccgg aaacgaagcc tgctctcctg ctacctgcag ctgccgatcc
     3601 tcaagggggc ggccgacggg tacgaagtgc acctcaggct ctccgaccag atggaggtcg
     3661 agaaagactc atcggtcccc tcgcccgggg cctatgcctc cggcgcctgg gtctggaaat
     3721 gcggcccgga tgaaacctgc cccgctttcg ccgcagtcag ctactcgatc gaggacggca
     3781 ccaagcaggt gatcatcctg gtcctcgcct ccgtcttcgg cgcgttgatc gcactgctgg
     3841 tcggcgaggt actgatcgag tgggcgagaa aacgcttccg cgaaccggac tgagcggttc
     3901 agcccgggag cgccgtatcc aacggcaggg cgggcagcga agccagttcg gagcggtcca
     3961 gcggccgggg ccggccgaga tagtggccct ggacgaaatc gaccccgaac tcgcggagca
     4021 cctccagggt cctcgcgtct tcgacctgct cggcgaccgt ccgcttgccc atgccttttg
     4081 aaatctccac cagggccttg accagcagcc ggttgaccgg gtccgaggcc aggttctgga
     4141 cgaactcgcc gtcgatcttc acgtagtcga acgccagatg cttgaggtaa tagaaggacg
     4201 cgaatccggt cccgaaatca tccagggcga aaccgcagcc gagctcgttc atacgggccg
     4261 agaactcctg ggccccggtc atgttgccga tcgcgctggt ctcggtcact tcgaagatca
     4321 ggttggacgg gtccaccccc gccctcgaga gctcgccctg cacgaattcg ggcagcttcg
     4381 ggtccccgac cgtgcgtccg gacaggttga tctggagccg cgggcaaccg tccccttcga
     4441 gctcggccag ggtcgaaatc gccttgctga tcacccatga atcgagttcc tggaccaggt
     4501 catggcgttc ggcggtcggc aggaaggcgc cgggcatgat gatcgaaccg ctgcgatcgc
     4561 gcatccggac gaaaagctcg tgatgcggcg ggccctctcc gttgagccgg tgaatcggct
     4621 gcgcgtagac cagcagctct tcgttttcaa gcgcgctgcg cagcttcccg gcccagtcga
     4681 tgcccagcgt gacatgtgaa tcctcgccgg gctgaaagac cctggcaccg ttgcggccgg
     4741 cgtccttggc ttcgtacatg gcgatgtcgg cctgcatcat cagcgtttcc tcgggcaccg
     4801 gcgagatctc cgacgaaacc gcgacgccga tgctggccgt aacccgggca gaatgttcgg
     4861 ccccgacgat ccgcacgtcc cgggcgatca gcctgaccag ttcctcggcg gtggtcaaag
     4921 cgtcttcggg gctgatgtcg cgaagcacca ccacgtattc gtcgccaccc tgccgggcga
     4981 gggtgtcatc ggagcgtagc cggctcttca acagctcggc cgtccgcacg atcaaccggt
     5041 cgccgatcgt gtggccgaaa ctgtcgttga cgaacttgaa gttgtcgagg tcgatgacca
     5101 ggatcgcgat cccccggccg tgtccggagt cgatcgccga gctgagttcc tcttcgaagc
     5161 gacgccggtt gtagaggccg gtcagcgggt cgtgatcggc cagatacttg agctgctggc
     5221 tgagccgctg gcgttcggtg atgtcttcga ccatcgacag tgcctggttg ggtttgccgt
     5281 cctgatcgcg aagcatggtc acggtcaggt agatccagac cacctccccg ttcttgcgga
     5341 tgtaccgctt ctccatgctg aagccgtccc gctccccggc gaagagttcg ccggtcatcg
     5401 cgacgtcctc ttcgaggtca tccggatggg tgatgtcggc aaaggtcatt tccgagagct
     5461 cacccgcgga gtagccggtg atcgactcga agctccggtt caccttctcg aaacggccct
     5521 cgggagtgac caggcaaaga ccgatcgggc tctgctcgaa aacctgccgg aagcgcatct
     5581 cggcgtcgcg cccggccgat ttcgcgattc gttccccggt cacgtcgtac ttgacgagga
     5641 tcagaaccgg cccgtgttcc ggcacggtcg tgatcacgcc gttgacccgg ctccagatgt
     5701 cggtgccatc cttcctgacc gagcggttct cgtagctggt ctcccccttg tcggccagtt
     5761 cgtgaagcac gtcgacgcgc atctggactt catcctcgga gagatcgtcc ggggcgatca
     5821 catcgatgtt ccggccgatc aactcgcccc ggtcgaaccc gtgcatctca tcgaactgcg
     5881 ggttggtcag cacgattcgc gaatcggcga tccgcaggac cacgacgccc tcggacatcc
     5941 ttgtcaggac gctggcctgc gaggcaagtt ccgaattggc ctgtttccag cggtcgtaga
     6001 agcgctgcag gcgaatcgag gcgtaggtga tcatccagat caggaggacc agcatcagca
     6061 ggcggtcgag ccccttgatg aacgcggact caccggactc gagcccttcc gagatcaggt
     6121 cgaccatgac catggtcagc gggatcacca ccgccgtcgg aacgagcagc cgggccattg
     6181 acggtccggg accggtcgat gagagaaaac cggccagccc ccggtcaggc cggaggaaca
     6241 cgtacccgac gtagatcagg attacggtcg ccgatccgag gatgctgatg tcggtctgaa
     6301 cgttggcatc gagcgcggcc tggtaaccct tggtcaggac cgagatcagg aagatgatgc
     6361 cgaccagcgg gatcagggcc cgctgccagc ggtagcgggg cggatcgcga tcgatcgtca
     6421 gcaggcagag tgccagggcc gggttgttga cgagcgcggc gaccgacggc gagcggagcg
     6481 cgtccgaacc ccacacgtcc ggatcgatac caaccatccg cagggccagc agcgccatgc
     6541 agacggcgat cgtgaagtag cagagcgcca cgccggcctg gaccgccgat tcggcagcgc
     6601 cggggcgcat cgccagcagg gcgaaggcga ccattcccag ggtcaccgac gagaacaccg
     6661 gcatctgcgg agcggcgaga agctcgcccg tgaacaacgg gatgtcgacg aaacctgcca
     6721 aggtcgccag caggcagatc accagggcgg cccagccgag cacggtcggg gcccgcttga
     6781 tcccggtggt ccagagccag gtgtagctgc cggggccggc cgcttcggcc cagatcccgg
     6841 cggggtcggg ctccttttcg agcgccgccc ggctcgcagc ctcggctatc cgcccccggt
     6901 acagcttcag ctcgaccgga tcgggaatgt tccgatcttc ccgctcatct ggattcgaca
     6961 agtgcttctc caaaggaact cctgactgcg aaactaacac ctgctttccc gggaacggga
     7021 acgccgaaac gcatggtggc cgcggttcag gcggtggcgg cttcggccgt ctccctggcg
     7081 acctgatcgg cctcttccca ctcggcgtag agcgaggcta ccttctcctt cgcccggtca
     7141 tgccgccggg ttgccttctc cgacttctcg ggagtcgacc aggccgcggg atcggagagt
     7201 tcgtcctcga tttcagccag ccgggcctcg gcctgttcga tcttcttttc gaggttctcg
     7261 acccggcgcc gggttcgggc cacctccttg ccgggcgagc cgccgcgccg ggcgttgccc
     7321 gacttcccgg ccggccggga agcggcagct ttttcctgtt cggcctccgc ggcttcatcc
     7381 cggcgcttct gatactccgc ccagccggtc atgtggtttg tcagttcgcc gttctcgcag
     7441 accagggtcc ggctgccgac cgcttccagc agggcgcgat cgtgagaaat gaggacgacc
     7501 gccccgggga aaccgagcag ggcatcctcg agtgcttccc ggctttcgat gtccagatgg
     7561 ttggtcggct cgtcaagcag caggaggttg gctccggagg cgaccaggat cgccagcgag
     7621 agccgccgtt gctcaccgcc cgagatgtcg ctcagctgct tgttcgcgtc ttcgcccgag
     7681 aagagaaagg cgccgagcag gtcccggacc ttctgtccgg tgagcccggt ttcgcgggcg
     7741 gccgcgtcga gcacggtgcc gtcg
//
LOCUS       DAOQKN010000122         7708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_4629_length_7708_cov_5.842545, whole genome shotgun sequence.
ACCESSION   DAOQKN010000122 DAOQKN010000000
VERSION     DAOQKN010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 7708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7708
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4629_length_7708_cov_5.842545"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /locus_tag="PLJ64_08610"
     CDS             <1..962
                     /locus_tag="PLJ64_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93583.1"
                     /translation="LRWEPDGTESRLPMADRLVKVAESSEDPEDLAEALSWRSVVQVC
                     LGHRAEADADLARFDVLAKTLPQIRVNQAALHTARKVREGRWAEAEQLIAKTIAQEIP
                     NTARIALTDPLLFMIRAEQNRLGEHFDQVKAMAAQSTSWEHWPTWRMGYYLAMTQCGR
                     GDEVLERVDRFNQADVEAIETRNITFLSFCALGSMVACEIEFRRGAETLVNLMEPYVG
                     EWVITGPAGSALGPVEMHLGEICLLLGRDREAATWLERSVDNCEGMGARPYLAHSKMH
                     LALALRRLGNPEPERAEELMSSGQQIAEELDMQMLLNRINRWS"
     gene            complement(1099..1809)
                     /locus_tag="PLJ64_08615"
     CDS             complement(1099..1809)
                     /locus_tag="PLJ64_08615"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNC93584.1"
                     /translation="MSLTTYGVDDRGVALIQLDRPKAMNAINTEMLVELIEHVAVARG
                     DEGVKVLVFSSTDYMAFSAGADIKEDIDDEAKVNKMQLFADLYDAVVQFPKPTIAACH
                     GYVVGGGAEIAAACDIRIGGSNLQMRFPGAALGVPVGPARLVTLCGLSHAKYLLLSSN
                     TVKVDEALRMGLINQAAPGAATENTALELAGRIAAHDPAGVARLKRMLHEWDDIEGRS
                     EAEGRGQVEWQRSGPGLH"
     gene            complement(1831..2388)
                     /gene="pth"
                     /locus_tag="PLJ64_08620"
     CDS             complement(1831..2388)
                     /gene="pth"
                     /locus_tag="PLJ64_08620"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNC93585.1"
                     /translation="MVGLGNPGREYERSRHNAGFMVAARLAERWEMPRAKDKFGARIT
                     EGRIRPGGPRVVLMTPLTYMNESGKSAGPARGSKKIPLDRVIAVYDEIDLPFGEIRTR
                     LGGGAAGHNGVKSLKQGFGSAGFWRVRIGVDRPDSTDPERVAAYVLGKFSESREQVEE
                     LIDDAARETERLVERIAAGETVDGD"
     gene            complement(2446..3135)
                     /locus_tag="PLJ64_08625"
     CDS             complement(2446..3135)
                     /locus_tag="PLJ64_08625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNC93586.1"
                     /translation="MSSDRATLNATTRTDFGSRISKRLRRDGKVPGVVYSDGKEATTF
                     QVESRDARVILGEGHALFDLHIEGSDAVPVVVKEQQHHPVRGDLQHLDLQQVDLNQAI
                     QAEVAVELTGDDVSPGVKQGGVLEHVTREVTVEALPTDIPDSITLDVSEMEINDTLTL
                     EQLVAPEGVTLIADDPAEVTLVTLSPPRVEEEPEVDLEAEPEVVGEGEEGGEEAAEGE
                     EGGDEGSSGEE"
     gene            3174..4601
                     /locus_tag="PLJ64_08630"
     CDS             3174..4601
                     /locus_tag="PLJ64_08630"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HNC93587.1"
                     /translation="MADDSKIPKGRLRRSAKLGTAIGSQGAKYAGTKASGLFRGEDSS
                     QEKLDERHAETAAKMVATLGQMKGAAMKIGQFASFIDIDFIPEEYREIYQEQLAKLRS
                     EAPSMPWEEVVKVLKEEYDGEPIEQLFDSIEHEAFAAASIGQVHRGRLIDGREVAVKI
                     QYPGIAEALESDLKNAGMLVRMARALAPGLDAREVTRELRERVLEELDYEYEAQNQRS
                     FARAYRGHPFIYVPDVITRLSRRRVLVTEFVEGIGFEEILELGDEQRSRFGEILFRGS
                     FGSIYHLQHFNADPHPGNYLLMKDGRVAFLDFGMTKKLDAEQIELEQLAVDAAVRNDP
                     EGLRDALHNLGFVKKPSKLDAEHLMNHVMSIGGWYMEDREIEIDGDRVMKVIESTHDP
                     RSEYFDLMRRESIPADELMGRRMEIGVLAVLGRLRAKRNWHRIMREWVYADPPATELG
                     REEWEYFEERGIFQVPGIKKGKLES"
     gene            4598..5440
                     /locus_tag="PLJ64_08635"
     CDS             4598..5440
                     /locus_tag="PLJ64_08635"
                     /inference="COORDINATES: protein motif:HMM:NF039362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6159 family protein"
                     /protein_id="HNC93588.1"
                     /translation="MKRIKAGWQLTKKSWRVLSDSPGLVRFPLIGGLIALLITIVLIG
                     PGLYLLEDGNSAPGAILAAVGIYLAAFATFYFAVGLAHNADRQMHGETATFGDGMALA
                     SSRMGAIAGWAFVATVVMTIIRAIQERFGIAGAIVGGLAGAAWGILTFLAVPVIALEG
                     TGPIETIKRCASLVKSRWGEQIAGTIAIGGIVFLVGFLPATLLIVAGVALWTVSGIGG
                     AILIAVGVLVLVVAALVQQSLATIFGVALYRYVAGQEAVGGFTEQELESAVRLKRGAG
                     PATI"
     gene            complement(5456..6136)
                     /locus_tag="PLJ64_08640"
     CDS             complement(5456..6136)
                     /locus_tag="PLJ64_08640"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC93589.1"
                     /translation="MDLPEKSDAVLSQPTRARIFELLAESARPVPTTWLADQLGLHPN
                     GVRAHLDRMLQAGLVERRRDAAGRGRPRDEWTLSPDARPGGSPPTAYSDLVRWLARAY
                     PAGTESLRRIEAVGREAGRDAGRKAAPGGSSSPAEALAVTLTAFGFQPRVTGESDEGF
                     TCCLGNCPYREAVHENQLVVCGLHRGLTGGLLETLEPGFRLEGFEPRDPDEAGCVITA
                     IRQTGEPC"
     gene            complement(6149..7375)
                     /locus_tag="PLJ64_08645"
     CDS             complement(6149..7375)
                     /locus_tag="PLJ64_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93590.1"
                     /translation="MSSGTMRPTDLLLASVLTGTAFLAAAGALALAGEFAGWGTAGWM
                     ALHLALLGGVSQLVIGVSQFFVTAFLATTPPPPKLVAAQTGCWSVGTVAVVTGVAADV
                     EPAVLAGSALLLTTLGLYAAALSGLHRRSLQRAAWASRWYAASAAWLVPGIVAGVLLA
                     TGVAWPYGSLLGAHLAFNLGGWLGGAIVGTLHTFFPSLTRTGLRFGRLQPLTFLLWSA
                     GVGSLAIGYGAGEEPLVLAGWALLLLASGSLSVNLIASAVAAPAGLTLPGRLVASAQV
                     FLPLGVAFALLSALEHPLAPLAGKDQGVLAVLLVVGWIGLTVAGSMLHLISVVVHVRD
                     LGRGGPKKDSWRDRAVSWLAVAGVALLSVARIADLDQLTGPALLLIFAAGLMILSSLL
                     RSLVSAIRLGPGDRFG"
     gene            7442..7705
                     /locus_tag="PLJ64_08650"
     CDS             7442..7705
                     /locus_tag="PLJ64_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNC93591.1"
                     /translation="MAYVINEPCIAVKDNSCVEVCPVDCIHPTPDEPDYENVDQLFID
                     PEECIDCDACVEACPVDAITPEDMVPPEWQRFIEINANYYKDK"
BASE COUNT         1345 a         2529 c         2594 g         1240 t
ORIGIN      
        1 ttctccgctg ggaacccgac gggaccgaat ccaggctgcc gatggcagac cggctggtca
       61 aggtggccga atcttccgag gatccggaag acctggccga agccctgagc tggcggtcgg
      121 tcgttcaggt ctgtctcggt caccgggccg aggccgatgc cgacctggcc cgcttcgatg
      181 ttctggccaa gaccctgccc cagatccggg tcaatcaggc cgccctccac accgcccgga
      241 aggtccgcga aggacgctgg gccgaggccg agcagctgat cgcgaaaacc atcgcgcagg
      301 agatcccgaa caccgcccgg atcgccctga ccgacccgct gctcttcatg atccgggccg
      361 agcagaaccg gctcggcgag catttcgacc aggtcaaggc gatggctgcc cagtcaacga
      421 gctgggagca ctggccgacc tggcggatgg gctactacct ggcgatgacc cagtgcggcc
      481 gcggggacga ggtcctcgaa agggtcgacc ggttcaacca ggcggacgtg gaagcgatcg
      541 aaacccgcaa catcaccttc ctctcattct gcgcgctcgg ctcgatggtc gcctgtgaga
      601 tcgagttcag gcgaggggcg gaaaccctgg tcaatctgat ggagccttac gtcggtgaat
      661 gggtgatcac cggaccggca gggtcggcgc tcggcccggt ggaaatgcac ctgggcgaga
      721 tctgcctgct gctgggccgg gaccgtgagg ccgccacctg gctggaacgc tcggtcgaca
      781 actgcgaagg gatgggcgcc cggccctacc tcgcccactc gaagatgcac ctcgctcttg
      841 ccctccggcg cctcggcaac cccgaaccgg aaagagccga agaactaatg agctccggcc
      901 aacaaatagc cgaagaacta gacatgcaga tgctcctgaa ccgcatcaac cgctggtcct
      961 gaagaacctc cggcagagcc ggagcaaagc tcgccgactc cctaatgctg ggcccggtgt
     1021 cgggggtgcc tgaaaagtga ctccttaatc agagcgcgaa gcgctcgtta cttttcaggc
     1081 acccccgaca ccgggccctc agtgcaagcc agggccggaa cgctgccact cgacctggcc
     1141 ccggccttcg gcttccgacc gcccctcgat gtcatcccac tcgtggagca tccgcttcaa
     1201 tcgggcgaca cctgcggggt catgggcggc gatccggccg gccagctcga gcgcggtgtt
     1261 ctcggttgcg gcgccgggtg cggcctggtt gatcaggccc atcctcaggg cttcgtcaac
     1321 cttgaccgtg ttcgaggaga gcagcaggta cttggcgtgg ctgagcccgc aaagggtgac
     1381 cagccgggcc gggccgaccg gcactcccag cgccgccccg gggaatcgca tctgcaggtt
     1441 cgagccgccg atccggatgt cgcaggcggc cgcgatctcg gctccgcccc cgaccacgta
     1501 accgtggcag gcggcgatgg tcggtttggg aaactggacg accgcgtcgt agaggtcggc
     1561 gaagagctgc atcttgttga ccttggcttc gtcatcgatg tcttccttga tgtcggcgcc
     1621 ggccgagaag gccatgtaat cggtcgagga gaagaccagc acctttacgc cttcgtcacc
     1681 gcgggccacc gcgacgtgct cgatcagttc gaccagcatt tccgtgttga tcgcgttcat
     1741 cgccttgggc cggtcgagct gaatcagggc aactccgcgg tcgtctactc cataggtggt
     1801 caggctcatg gcagcttcac tttcctcggt tcagtcgccg tcaacggttt ccccggcggc
     1861 gattcgctcg acaagtcttt cggtttccct ggccgcatca tcgatcagtt cctcgacctg
     1921 ctcccggctc tcggagaact tcccgagcac gtaggctgcg acgcgttcgg gatcggtcga
     1981 atcgggtcga tcgaccccga tccggacccg ccagaaaccg gccgatccga aaccctgttt
     2041 caggctcttg accccgttgt ggccggcggc accgccgccg aggcgggtgc ggatctcacc
     2101 gaaggggagg tcgatctcgt cgtagacggc gataacccgg tcgagcggga tcttctttga
     2161 cccgcgagcc gggccggccg acttcccgga ctcgttcatg taggtgagcg gggtcatcag
     2221 caccactcgc gggccgccgg gccggatccg gccctcggtg atcctggcgc cgaacttgtc
     2281 cttggcccgc ggcatttccc agcgctcggc cagccgggcc gcgaccatga aaccggcgtt
     2341 gtggcggctg cgctcgtact ccctgcccgg gttgccgagc ccgaccacga ggatcggggc
     2401 atcggaccct tcttccgggt cggagcggcg caggaaagac agggcctact cctcgccgga
     2461 ggagccttcg tcgccgccct cttcgccttc ggcagcttcc tcgccgccct cttcgccttc
     2521 gccgaccact tccggctcgg cttccagatc cacttccggc tcctcttcga cgcgcggcgg
     2581 cgagagggtc accagggtga cttcggcggg atcatcggcg atcagcgtga cgccttcggg
     2641 cgcaacgagc tgctcgaggg tgagcgtgtc gttgatctcc atctcggaga cgtcgagggt
     2701 gatcgagtcc gggatgtcgg tcggcagcgc ctcgacggtc acttcgcggg tgacatgctc
     2761 gagcacgccg ccctgcttga cgccggggct gacgtcgtca ccggtcagct cgaccgcgac
     2821 ctcggcctga atcgcctggt tgaggtcgac ctgttggagg tcgaggtgct ggaggtcgcc
     2881 gcgcaccggg tggtgctgct gctccttgac cacgaccgga accgcatcgc tgccttcgat
     2941 gtgaagatcg aacagcgcgt ggccttcacc caggatcacc cgggcgtcgc gactttcgac
     3001 ctgaaaggtg gtcgcttcct tgccgtccga gtagacgacc ccggggacct tgccgtcacg
     3061 gcggaggcgc ttggagattc gggagccgaa atcggtgcgg gttgtggcgt tgagtgttgc
     3121 tcgatctgaa gacattggac agaacaggat agggaaagtt ctacgcttga ggcatggcgg
     3181 acgattcaaa gatccccaag ggcaggctcc gtcgctcggc aaagctgggc acggcgatcg
     3241 gctcgcaagg cgccaagtac gccgggacaa aggcctcggg cctgtttcgg ggtgaagatt
     3301 cgagccagga gaagctggac gaacggcatg ccgaaaccgc cgcgaagatg gtcgccacgc
     3361 tcggtcagat gaagggcgcg gcgatgaaga tcggccagtt cgcctccttc atcgacatcg
     3421 acttcatccc cgaggagtac cgcgagatct accaggagca gctggcgaaa ctgcgttccg
     3481 aagcgccttc gatgccctgg gaagaggtcg tcaaggtgct gaaggaggaa tacgacggcg
     3541 agccgatcga gcagctcttc gactcgatcg agcacgaagc tttcgccgcc gcctcgatcg
     3601 gccaggtcca ccgcggccgc ctgatcgacg gacgcgaggt cgccgtcaag atccagtacc
     3661 cggggatcgc cgaggcgctg gagtcggatc tcaagaatgc cgggatgctg gtgcggatgg
     3721 cccgggcgct cgccccgggg ctggacgccc gggaagtcac ccgcgaactc cgcgaaaggg
     3781 tgctggaaga gctcgactac gaatacgaag cccagaacca gcgttccttc gcccgcgcct
     3841 accgtggcca tccgttcatc tacgtccccg atgtgatcac tcgcctttcc cggcgccggg
     3901 tgctggtcac cgagttcgtc gaggggatcg gcttcgagga gatcctcgaa ctcggtgacg
     3961 agcagcgcag ccgtttcggc gagatcctct tccgcggcag cttcggctcg atctaccacc
     4021 tgcagcactt caacgccgac cctcaccccg gcaattacct gttgatgaag gacggccggg
     4081 ttgccttcct cgatttcggc atgaccaaga agcttgacgc ggagcagatc gagctcgaac
     4141 agctggcggt cgacgcggcg gttcgaaacg atccggaagg ccttcgcgac gcgctccaca
     4201 acctgggctt cgtcaagaag ccgtcgaagc tggatgccga gcacctgatg aaccacgtga
     4261 tgtcgatcgg tggctggtac atggaagacc gcgagatcga gatcgacggc gaccgggtga
     4321 tgaaggtgat cgagtcaacc cacgatcccc gctccgagta cttcgacctg atgcgacggg
     4381 aatcgatccc ggccgacgag ttgatgggcc gccggatgga gatcggtgtg ctggccgtgc
     4441 tcggccggct gcgggcgaag cgcaactggc accggatcat gcgcgagtgg gtgtacgccg
     4501 acccgccggc gaccgagctg ggcagggagg aatgggagta cttcgaagag agagggattt
     4561 ttcaggtccc cgggatcaag aagggtaaat tggagtcatg aagcgcatca aagctggttg
     4621 gcagttgacc aagaaaagct ggcgcgttct ttcggactcg cccggcctgg tgcggttccc
     4681 gctgatcggc ggcctgatcg cactcctgat cacgatcgtg ctgatcggcc ccgggctcta
     4741 cctgctcgag gacggaaaca gcgctcccgg ggcgattctc gcggcggttg gcatctacct
     4801 tgcggccttt gccaccttct acttcgcggt cgggctcgcc cacaacgccg accggcagat
     4861 gcacggggaa accgccactt ttggcgacgg catggctttg gcctcgagcc ggatgggagc
     4921 gatcgccggc tgggccttcg tggcgaccgt cgtgatgacg atcatccggg cgattcagga
     4981 gcgcttcgga atcgccggcg caatcgtcgg tggtctggcc ggcgccgcct ggggcatcct
     5041 cactttcctc gccgtcccgg tcatcgctct cgaaggcacc gggccgatcg agacgatcaa
     5101 gcgttgcgcc agtctggtca agagccgctg gggcgagcag atcgccggca ccatcgcgat
     5161 cggcggcatt gtctttctgg tcggcttcct gccggccacg ctgctgatcg tcgccggggt
     5221 agcgctctgg accgtgtcgg gtatcggcgg cgcgatcctg atcgccgtcg gggtgctcgt
     5281 cctggttgtg gccgccctgg tccagcagtc gctcgcgacg atcttcgggg tcgcgctcta
     5341 ccgctacgtg gccgggcagg aagcggtcgg cggattcacc gagcaggagc tcgagagcgc
     5401 ggtccggctc aagcgcggcg ccgggccagc cacgatctga cccgggggat ccgcctcaac
     5461 aaggttcgcc ggtctgcctg atcgcggtga tcacgcagcc ggcctcgtcc ggatccctcg
     5521 gctcgaaccc ttcgagccgg aatccgggtt cgagagtttc cagcaggccg ccggtcagtc
     5581 cgcggtggag tccgcagacg acgagctgat tctcgtgaac ggcctcgcgg taggggcagt
     5641 ttccgaggca gcaggtgaaa ccctcgtcgc tttccccggt gacccgcggc tggaagccga
     5701 aggcggtgag ggttacggcg agggcctcgg ccggtgagga ggagccacct ggcgcggcct
     5761 tccggcccgc gtcccgcccc gcttcacggc cgaccgcctc gatcctgcgc aggctttcgg
     5821 tcccggccgg gtaggcccgg gcgagccacc tgaccaggtc cgagtaggcg gtcgggggag
     5881 aaccgcccgg ccgggcatcc ggcgagagcg tccattcgtc cctcgggcgt cccctccccg
     5941 ccgcatcccg gcgccgttcg accagacccg cctgaagcat ccggtcgagg tgggcccgga
     6001 cgccattcgg gtggaggccc agctggtcgg caagccaggt tgtcggcacc ggccgggcgg
     6061 actccgcaag cagctcgaag atccgggccc gggtcggctg gctcagcacg gcatcgctct
     6121 tttccggcag gtccacggac tcagcctctc acccgaagcg gtctccgggc ccgagccgga
     6181 tcgcgctgac caggcttcgc agcaggctcg agagaatcat cagcccggcg gcaaagatca
     6241 gcagcagggc cggtccggtc agctggtcca ggtccgcgat ccgggcaacc gaaagcagcg
     6301 ccaccccggc cacggccagc cagctgaccg ctcgatcccg ccacgagtcc tttttcggcc
     6361 cgccccgccc gaggtctctc acatgcacga ccaccgagat caggtgaagc attgaaccgg
     6421 cgacggtgag gccgatccat ccgaccacga gcagcaccgc gaggacgccc tgatccttgc
     6481 cggccagcgg agccagggga tgctcgagtg cggaaagcag ggcgaaggcg acgcctagcg
     6541 gcaggaagac ctgggcggag gcgaccagcc ggcccggcag ggtcagcccg gccggggcgg
     6601 caaccgccga ggcgatgaga ttcacggaaa gcgagcccga agccagtaac agcagcgccc
     6661 agccggcgag gaccaggggt tcttctccgg cgccgtaacc gatcgccagc gatccgacgc
     6721 cggcgctcca gagcaggaag gtcaggggct gaaggcggcc gaaacgaagc ccggtccggg
     6781 tcagggacgg gaagaaggtg tggagggtgc cgacgatcgc accacccagc cagccgccca
     6841 ggttgaaagc gagatgggcg ccgagcaagg agccgtaggg ccaggcgacg ccggtcgcga
     6901 gcaggacgcc agccacgatg ccgggaacca gccaggcggc gctcgcggcg taccaccggc
     6961 tggcccaggc ggcccgctgg agggagcggc gatgcagccc ggaaagggcc gccgcgtaga
     7021 gcccgagggt ggtcaggagg agtgcggaac cggcgagcac ggccggttcg acgtccgcgg
     7081 caaccccggt cacgaccgcg acggtaccga cgctccagca accggtctgg gccgcgacca
     7141 gcttcggtgg aggcggtgtg gtggcgagga aggcggtcac gaagaactgg ctgaccccga
     7201 tcacgagctg ggacaccccg ccgagcaggg ccaggtgaag tgccatccag ccggcggttc
     7261 cccagccggc aaactcaccg gccagcgcga gggctcccgc agcggccaga aacgcggttc
     7321 cggtgaggac ggaagcaagc agcagatcgg tgggccgcat ggttccggag gacatgacgc
     7381 gattttacac acaatgactg tgtaaaatgg tcgccccgaa tccgaatcga acgagcgtga
     7441 gatggcctac gtaatcaacg aaccctgcat cgccgtgaag gacaactcct gcgtcgaggt
     7501 ctgtccggtc gactgcattc atccgactcc cgacgagccc gactacgaaa acgtggacca
     7561 gctcttcatc gacccggagg agtgcatcga ctgcgacgcc tgtgtcgagg cctgcccggt
     7621 tgacgcgatc accccggagg acatggtccc gccggagtgg cagcgcttca tcgagatcaa
     7681 cgcgaactac tacaaggaca agtagtcc
//
LOCUS       DAOQKN010000123         7637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_4687_length_7637_cov_6.712609, whole genome shotgun sequence.
ACCESSION   DAOQKN010000123 DAOQKN010000000
VERSION     DAOQKN010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 7637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7637
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4687_length_7637_cov_6.712609"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /gene="gluQRS"
                     /locus_tag="PLJ64_08655"
     CDS             <1..773
                     /gene="gluQRS"
                     /locus_tag="PLJ64_08655"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HNC93592.1"
                     /translation="LGNLRTALIAWRSARDRGARFLVRIEDIDTGRSRDEWIEVQLEE
                     LRQIGVDWDGEPVRQSERHDLYREALSKLEADGLLYECFCTRAEIREAASAPHGELPE
                     GAYPGTCRELTEAERAGRRAAGRPPALRVRAAGARVGFDDHLCGRVEKTLDDFVVRRN
                     DGDFAYNLAVTVDDADQGIGEVLRGADLLDTTPRQLWLYDRLGLAAPARFSHVPLMLG
                     EDGERLAKRHGAITLEERIEAGESPASVRRELFDSADR"
     gene            860..2614
                     /locus_tag="PLJ64_08660"
     CDS             860..2614
                     /locus_tag="PLJ64_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93593.1"
                     /translation="MKKFILGMTLSLFMLGAAQADAVPKYNASCMFNGCGGVSQEGQR
                     AIFTFDDMYPGPDEDFDIYERVGGQTRPLIDYPDGKKGHALLQFVSDDARRAIVETRS
                     PLTADDTDGNGEDFFAYQDGVPELISWDPADPSTRAGDIDLQLINATDDGKTLYFFRF
                     NKATPLCHELWKRTPTSFSRVTNDCDLLWMRGLSRDGSSVFYDDYSNGLWRVKDDARV
                     PVNDQPHPVNVHCTKYNYFGDATEDGEAWLFSSSYRLADADTDDLFDIYLRHRDGSYE
                     LITDTADGAEPEDCTSQNWLTRAIGLSPDMTRALFVTEFPISPDDHDQSLDVYLHTPQ
                     GNELVSTSPTDQSPEIRNPTSVHLQGGRPDSEFRVDGSDDLSVIAFDSEQQLVPEDND
                     SDVDVYARIGDETRLVSTGPNPDRNSRKAKLLGVSNDGTTISFSTPEPLLPVDTDGRM
                     DIYAYSTSGLSRLGDAAASASRKKRRVVLVSAESDAPRMKVLGKPSVSGRRALVRLRC
                     PKSEMTGPCRGTVRIRFGGSGARGAARFRVKPGRSVRVGVKLNRRVAAGKGRAQVRIK
                     AWDRLGNTYRAVRKLSFR"
     gene            2716..3684
                     /locus_tag="PLJ64_08665"
     CDS             2716..3684
                     /locus_tag="PLJ64_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93594.1"
                     /translation="MYESWYARLVSPDGSLALWIRYTIDKLPNQEATGTIWFTLFDGE
                     AERPLARRLVGQPASAPVNTWARIGESTIGPAGLHGECFEAAWQLRFEPRAPVLFHLP
                     RPRFYELPLPKTKPISPVPFGDFSGSVNFGERQISVEGWHGMIGHNWGSEHAERWIWL
                     HGSDFDEDPEAWLDIVMGRIRVAGRLLPWVANGAISTGGEIRRIGGMFASGVNVKETP
                     LRLEAGLPVAGKSYLELEVDSPPNLTVGWQYGDPADPEAEAHEVANCSAAQMKATLTM
                     PGHNAVTNLGTLRGAVYELGMTETDHGIPISDGIPPGHGPGSAVSK"
     gene            complement(3722..4465)
                     /gene="heR"
                     /locus_tag="PLJ64_08670"
     CDS             complement(3722..4465)
                     /gene="heR"
                     /locus_tag="PLJ64_08670"
                     /inference="COORDINATES: protein motif:HMM:NF037099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heliorhodopsin HeR"
                     /protein_id="HNC93595.1"
                     /translation="MPKLRRLNVFAAIFFAAQVAVLLVVAEPVTLPVTGQFLTGPPGG
                     GAYGSTELFRLRIDLLVALFLGLAAFDHFVVATWLRGFYEKNIGRGRNPARWYEYSVS
                     ASVMIVLIAMLSGVTDVVALLAIFGVNASMILFGLAMERANAGRTEVDWSPFIYGCVA
                     GAVPWIAIVIQFILSGSNSDGVPGFVYGIFVTLFLLFNCFALNMWLTYRARGRWRDPL
                     FAEKVYIWLSITAKTALAWQVYSGALAGS"
     gene            complement(4495..6909)
                     /locus_tag="PLJ64_08675"
     CDS             complement(4495..6909)
                     /locus_tag="PLJ64_08675"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HNC93596.1"
                     /translation="MNCRGWVALVAATVVLCAPGGARAGELTGLVNPFVGTEASAPDF
                     GTGGGAGNTFPGATMPFGMVAFGPDTIPSLTNFTAGYTYSDDQIRGFSLTHFSGAGCA
                     LLQDVPILPVARSLSGSPVVAGSSDIDPSIVPTFSHRHEEARPGFYSVVLNPGKADQI
                     RSELTATTRTAMGRFTFSKANTGTLTLNAGGSTMANYLAAVKVDPKAREVTGTSESGR
                     FCWEPSKYKVYFAARFDRPFKGYGTWKGAALSPGSKQASDESPDAFNYKPVAGGPPFI
                     PGNPSTTAQAGAFVSFNTSGSRPVGMRVGISFVSVAQARKNLSESAGLTFGEVRDRAG
                     QIWRQEMGKVRVSGGRKEDRRTFATALYHSLLEPSVQSDVNGLYRGQDLKVHRVAPGH
                     AHYSDISGWDVYRSQIQLLAMIEPRRAADIAASLLRMEKQGGCLPRWPYATQNSNVMN
                     GDPSSPIIATIHALGVRGFDARDALRAMVKGADHPCHSDNADYTEREALDDYLDLGWI
                     SQERNVTSGQHSVSERSSPWGTASTTMEYALADFTVSRLARALGRDQLAARFLKRSGK
                     WRNLVNPASQRIEPRFADGSFLPGANASTEDGFVEGSSAQYGWFVPQNLAGRFATLGG
                     RKAALQKLDHFFTELNAGPGSPYAFLGNEPTLQTPWIYNWLGRPDKAQSIVRRAQIGL
                     YGAGPGGLPGNDDGGTMSAWFVLSALGLSPVIPGTDVMPLGSPLFPRASLNLQGHRLQ
                     VRAAGADRKRPYVKSLRISGGKWAKPWIHVRRLLRGGRMNWKLSSSPSAWGRGKTLAP
                     PSFGDR"
     gene            6982..7461
                     /locus_tag="PLJ64_08680"
     CDS             6982..7461
                     /locus_tag="PLJ64_08680"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNC93597.1"
                     /translation="MAKKKKAGRVSAGLILYRVGPGGLEVLIAHMGGPHWARKERGWS
                     IPKGEIDPGEEPLETARREFREEVGIEPPAGAPIHLGEITQRSGKRVLAWAIEGDFDP
                     TQQTSVMCEIEWPPKSGEKIEIPEVDRVEWVPPATAKNLVIDGQIPLFDRLEKLVGR"
BASE COUNT         1347 a         2494 c         2552 g         1244 t
ORIGIN      
        1 atctcggcaa cctgaggacg gccctgatcg cctggcgctc ggcccgagat cgcggggcgc
       61 ggttcctggt ccggatcgag gacatcgaca ccggccgctc ccgggacgag tggatcgaag
      121 tgcagctcga ggagctccgc cagatcggag tcgactggga cggcgagccg gtccgtcaga
      181 gcgagcgcca tgacctttat cgcgaggcac tttccaaact tgaagccgac ggcctgctct
      241 acgagtgctt ctgcacccgg gccgagatcc gcgaagcagc gtcggcccct cacggcgagt
      301 tgccagaagg tgcctacccc ggcacctgta gggaactcac cgaggccgag agggccgggc
      361 gccgggctgc cggccggccc ccggcactcc gggtccgggc ggccggagcc agggtcggct
      421 tcgatgatca cctctgcggc cgggtcgaaa agaccctcga tgacttcgtg gtgcgccgca
      481 atgacggtga cttcgcttac aacctcgcgg tcaccgtcga cgatgccgat caggggatcg
      541 gcgaggtgct tcgcggcgcc gacctgctcg acaccacccc ccgccagctc tggctctacg
      601 accgcctcgg ccttgccgcc ccggcccgtt tcagccatgt ccccctgatg ctcggcgaag
      661 acggagagcg cctcgccaag cgccacggcg cgatcaccct cgaggagcgg atcgaagcag
      721 gagagagccc ggcgtcagtc cgcagggagt tgttcgattc ggctgatcgg tagcctcaac
      781 gcaacattct tgaacatgcc gtgttcaatt ttgtccattg caatcgacgg agccggagcg
      841 ctccgaacag gggaaaatct tgaagaagtt catcctgggg atgaccctca gccttttcat
      901 gctcggcgcc gctcaggctg atgccgtgcc caagtacaac gcgagctgca tgttcaacgg
      961 ttgcgggggc gtctcccagg aaggccagcg tgcgatcttc accttcgatg acatgtaccc
     1021 cggccctgac gaggacttcg acatctacga gcgggttggc ggtcagaccc ggccgctgat
     1081 cgactatccg gacggcaaga agggtcatgc cctgcttcag ttcgtctccg acgacgcgcg
     1141 tcgcgcgatc gtcgagaccc ggtccccgct cacggctgac gacaccgacg ggaacggcga
     1201 ggacttcttc gcgtaccagg atggcgtccc ggagctgatc tcctgggatc cggcagaccc
     1261 ctcgaccagg gccggtgaca tcgacctgca gttgatcaac gccaccgatg acggcaagac
     1321 cctctatttc tttcgcttca acaaggcgac cccgctctgc catgaactct ggaagcggac
     1381 cccgacgagc ttctcgcggg tgaccaatga ctgcgatctg ctctggatgc gaggcctttc
     1441 gcgcgacgga agctcggtct tctacgacga ctattccaac ggcctgtggc gggtcaagga
     1501 tgacgcccgg gttccggtca acgaccagcc acatccggtc aatgttcact gcacgaagta
     1561 caactacttc ggcgacgcga ccgaggacgg agaggcctgg ctgttctcct ccagctaccg
     1621 gctggccgac gccgacaccg acgatctgtt cgacatctat ctgcgtcacc gggacggcag
     1681 ctacgagctg atcaccgaca ccgctgatgg agctgagccc gaggactgca ccagtcagaa
     1741 ctggctgacc cgggcgatcg ggctttcccc ggacatgacc cgggccctgt tcgtgaccga
     1801 gttcccgatc tcgccggacg accacgatca gtcgctcgat gtctatctcc acacaccgca
     1861 gggaaacgag ctcgtttcga ccagcccgac cgaccaaagc ccggagattc gcaacccgac
     1921 ctcggtccat ctccaggggg gtcggcccga ctcggaattc cgggtcgacg gctccgatga
     1981 cctctcggtg atcgccttcg attccgagca gcagctggtt cccgaggaca acgattccga
     2041 tgtcgatgtc tacgcacgca ttggcgatga gacccggctc gtctcgaccg gaccgaaccc
     2101 agaccggaat tcgcggaagg cgaagctgct cggcgtctcg aacgacggaa cgaccatttc
     2161 cttcagcact cccgaaccgc tgcttccggt cgacaccgac ggtcgcatgg acatctacgc
     2221 ctactcgacc agcgggcttt cgcgccttgg tgatgccgcc gcgtccgcca gccgcaagaa
     2281 gcgacgggtg gtactggtct cggccgaatc ggacgctccg cggatgaagg tactgggcaa
     2341 gccgtccgtt tcgggtcgcc gggccctggt caggctgcgt tgcccgaagt ccgagatgac
     2401 cggtccctgc cgggggacgg tgcggatccg tttcggcggc agcggcgcgc gcggtgcggc
     2461 ccgattccgg gtcaagccgg gcaggtcggt cagggtcggc gtgaagctga accgtcgagt
     2521 tgcggccggc aagggtcggg cgcaggtccg gatcaaggcc tgggaccggc tcgggaacac
     2581 ctaccgggca gttcgcaagc tttcttttcg ctgagaagcc aattccttcg gcgccgctct
     2641 gatagttcta ttcgggtggg ccggaggatc tcaccgactg acgcgcgatt cacagaggcc
     2701 gggccaggcg ccgggatgta cgagtcctgg tacgcccggc tggtttcgcc ggacggttcg
     2761 ctggcgctct ggatcaggta cacgattgac aagctcccga accaggaagc aaccgggacg
     2821 atctggttca ccctctttga cggcgaggcc gagcgaccgc tggcccgccg cctcgtcggc
     2881 cagccggcct cggccccggt caacacctgg gcccggatcg gggaatcgac gatcggtccg
     2941 gccgggctcc acggcgaatg ttttgaagcc gcctggcagc ttcgtttcga gcccagggca
     3001 ccggttctct ttcacctgcc gcggccccgc ttctacgaac ttcccttgcc gaagaccaaa
     3061 ccgatctccc cggtgccgtt cggggacttc tccggttcgg tgaacttcgg cgagcgtcag
     3121 atcagcgtcg aaggctggca cggcatgatc ggccacaact ggggcagcga acatgcggag
     3181 cggtggatct ggctgcatgg cagcgacttt gacgaggacc cggaggcatg gctcgacatc
     3241 gtcatgggcc ggatccgggt cgccggccgg ctgctgcctt gggtggccaa cggggcgatc
     3301 agcactggcg gcgagatccg gcggatcggc gggatgttcg cctccggcgt caatgtgaag
     3361 gagaccccgc tccggctgga ggccggcctg ccggtcgccg gcaagagcta cctggagctg
     3421 gaggtcgact cgccgccgaa cctcacggtc ggctggcaat acggtgaccc tgccgacccg
     3481 gaggccgaag ctcatgaagt ggcgaactgc tcggcggccc agatgaaggc gacgctcacc
     3541 atgccgggcc acaacgcggt gaccaacctt ggcaccctgc gtggcgcggt ctacgaactg
     3601 ggcatgaccg aaaccgatca cggcatcccg atttcggatg gcattccacc cggccacggt
     3661 cccggatcag cggtctcaaa atgagggaag gactccgcga aaagttctgg ccgggctggt
     3721 ctcaggaacc ggccagagct ccgctgtaga cctgccaggc gagcgccgtc ttggcggtga
     3781 tgctcagcca gatgtagacc ttctcggcga acagggggtc acgccacctg ccccgggcac
     3841 ggtaggtcaa ccacatgttg agcgcgaagc agttgaacag aagaaacagg gtgacgaaga
     3901 tcccgtagac gaaacccggc accccgtccg aattggaccc ggagaggatg aactggatca
     3961 cgatcgcgat ccagggcacg gcgcctgcca cgcagccata gatgaatggt gaccaatcaa
     4021 cttcggtccg tcccgcattc gccctttcca tggccaggcc gaaaaggatc atcgaggcgt
     4081 tgacgccaaa gatcgccagc agggctacga catcggtaac gccggagagc atcgcgatca
     4141 ggacgatcat caccgaggcg cttaccgagt attcgtacca gcgggccggg tttctccccc
     4201 tgccgatgtt cttctcgtag aagccgcgca gccaggtggc gaccacgaaa tggtcgaagg
     4261 cggcgaggcc gagaaagagc gcgaccagca gatcgatccg gagcctgaac agttcggtgc
     4321 tcccgtaagc gccgcctccg ggaggcccgg tcaaaaactg gccggtcacg ggcagggtga
     4381 ccggttctgc gactaccaga agaaccgcga cctgcgccgc gaaaaagatt gcggcaaaga
     4441 cattgagtct cctgagcttc ggcatggccg aaccctacaa acgggcgccc ccggtcagcg
     4501 gtcgccgaag gagggcggag ccagggtctt gccccggccc caggccgacg ggctggagga
     4561 aagcttccag ttcatgcggc cgccccggag caatcggcgg acgtgaatcc acggcttcgc
     4621 ccacttgccg ccgctgatcc tgagggactt cacgtacggc cgcttgcggt ctgcccccgc
     4681 cgccctgacc tgcagtcggt ggccctggag gttcaggctg gcccggggaa agagcgggct
     4741 gccgagcggc atcacgtcgg tgcccgggat caccggtgaa agtccgaggg cggaaaggac
     4801 gaaccaggca ctcatcgtcc cgccgtcgtc gttgccgggc aggccgcccg ggcccgcccc
     4861 gtaaagcccg atctgggccc ggcgcacgat cgattgcgcc ttgtcgggcc ggccgagcca
     4921 gttgtagatc cacggtgtct gcagggtcgg ctcattgccg aggaaggcgt agggcgatcc
     4981 cggcccggcg ttcagctccg tgaagaagtg atcgagcttc tgcagggcag ccttgcggcc
     5041 accgagggtc gcgaaccggc cggccaggtt ctggggcacg aaccagccgt actgggcgga
     5101 gcttccttcg acgaatccgt cctcggtcga ggcgttggcg ccgggcagga agctgccgtc
     5161 ggcgaagcgt ggctcgatcc tttgactggc cgggttgacc aggttgcgcc acttgccgga
     5221 acgcttgagg aaccttgctg ccagctggtc gcggccgagt gccctggcca gccgcgagac
     5281 cgtgaagtcg gccagggcgt actccatcgt ggtcgaggcg gtgccccagg gggaggatcg
     5341 ctcggagacc gagtgctggc cgctggtcac gttgcgctcc tgggagatcc agccgaggtc
     5401 gaggtaatcg tccagcgcct cccgctcggt gtagtcggcg ttgtcggagt ggcagggatg
     5461 gtcggctccc ttgaccatcg ccctcagcgc gtcccgggcg tcaaaacccc ggaccccgag
     5521 ggcgtggatc gtcgcgatga tcggcgagga agggtccccg ttcatcacgt tcgagttctg
     5581 ggtcgcgtag ggccagcgcg gcaggcagcc gccctgcttc tccatccgga gcagtgacgc
     5641 cgcgatgtcc gcggcccgcc ggggctcgat catcgcgagc agctggatct ggctgcggta
     5701 gacatcccag ccggaaatgt ccgagtaatg ggcgtgaccc ggggcgaccc ggtggacctt
     5761 gaggtcctgg ccgcggtaga ggccgttgac atccgactgc acgctcggtt ccagcaggga
     5821 gtggtagagc gccgtcgcga aggtgcggcg gtcttccttc cggcccccgg aaacacgcac
     5881 cttccccatc tcctgccgcc agatctggcc ggcccggtcg cgaacctcgc cgaaagtgag
     5941 gccggcgctt tcgctcaggt tcttccgcgc ctgggcgacg ctgacgaagg agatcccgac
     6001 ccgcatcccg accgggcgcg aacccgaggt gttgaagctg acgaaggccc cggcctgggc
     6061 ggtggtgctc gggttgccgg gaatgaacgg tggcccgccc gcgaccggct tgtagttgaa
     6121 ggcatcgggc gattcgtcgc tggcctgctt cgacccggga gaaagggccg cgcccttcca
     6181 ggtgccgtac cccttgaacg gacggtcgaa cctggccgcg aaatagacct tgtatttcga
     6241 cggctcccag cagaaccgac cgctctccga ggttccggtc acctcacggg ctttcggatc
     6301 gaccttcacc gcggcgaggt agttggccat cgtgctgcca cccgcgttca gggtcagggt
     6361 cccagtgttc gccttcgaga aggtgaagcg gcccatcgcg gtgcgggtgg ttgcggtcag
     6421 ttccgagcgg atctggtcgg ccttgccggg gttgaggacc acgctgtaga aaccgggccg
     6481 ggcctcttcg tggcgatggc tgaaggtcgg gacgatcgac gggtcgatgt cggaggaacc
     6541 ggcgacgacg ggtgaaccgg acaggctcct ggccacgggc aggatcggca cgtcctgcag
     6601 cagggcgcag ccggcaccgc tgaagtgggt cagcgagaac ccgcggatct gatcgtccga
     6661 atacgtgtat ccggcggtga agttggtcag gctggggatc gtgtcgggac cgaaggcgac
     6721 catcccgaag ggcatggttg cgcccgggaa tgtgttgccc gcaccaccgc cggtgccgaa
     6781 gtctggagcc gaggcttccg tgccgacgaa ggggttgacc agaccggtca actcaccggc
     6841 cctcgctccc cccggagcac acaaaaccac tgttgccgcg accagcgcga cccatcccct
     6901 gcaattcacc caatgcaaat tacacgccat accccctttc gagggagcgc ccgggtcgcc
     6961 gggaaagtta gcctcggccc gatggccaag aagaagaaag ccggccgggt cagtgccgga
     7021 ctgattctct accgcgtcgg accggggggt ctcgaggtgc tgatcgccca catgggcggt
     7081 ccgcactggg cccggaaaga gcgcggatgg tcgatcccga agggggagat cgaccccggc
     7141 gaggaaccgc tggaaacggc ccggcgcgag tttcgggaag aggttggaat cgagccgccg
     7201 gccggggctc cgatccacct tggcgagatc acccagcgct ccggcaagcg ggtgctggcc
     7261 tgggcaatcg agggggactt cgacccgacc cagcagacga gcgtgatgtg cgagatcgag
     7321 tggccaccga agtcaggcga gaagatcgag attcccgagg tcgaccgggt cgagtgggtg
     7381 ccgccggcca ccgcgaagaa cctggtcatc gacggccaga tccccctctt tgaccgcctc
     7441 gaaaagctgg tcggccgcta gaacctagac ttctccccga tgaagacggc cgcgaagttg
     7501 ctgagcctgg ggctgacggt cggggtgctc gcaacgaccg cgaccgtccc tgcctcggcg
     7561 gccgatacct acctcgattg ctcggcggcg attgacggag acgggtcgcc ggcccagcca
     7621 cgcaactcgc tcggcag
//
LOCUS       DAOQKN010000124         7489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_4809_length_7489_cov_5.724374, whole genome shotgun sequence.
ACCESSION   DAOQKN010000124 DAOQKN010000000
VERSION     DAOQKN010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 7489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7489
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4809_length_7489_cov_5.724374"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(262..1218)
                     /locus_tag="PLJ64_08685"
     CDS             complement(262..1218)
                     /locus_tag="PLJ64_08685"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HNC93598.1"
                     /translation="MSDRAEIEASLQRIAGKQFGLVTRTQMLGASLSKAMIKHRQDQN
                     RLLPAGRAVFAVGRRAEGRLAACMAATLVAGGGAVLSGRAAADVWDIRKDRGNLEVKR
                     RNSHRSGEFVLDRRGVAEARPVRVRRSRYFNPAQVTRKYGIPILRPSWILLDLARELP
                     DDEFGHAFKEADRLGLLNEEELRACLELGSGLEGIRKFQNLVARRHPDLKDVRTLLET
                     LFLELCARYGIETPRVNRRKGRYFPDFSWDDIGLIVEVDGYEGHAGRFAFLDDAAREN
                     DLRRLGYQVIRFTWEEITEHPELVASLVIREIARCRALARVA"
     gene            complement(1441..2001)
                     /gene="thpR"
                     /locus_tag="PLJ64_08690"
     CDS             complement(1441..2001)
                     /gene="thpR"
                     /locus_tag="PLJ64_08690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HNC93599.1"
                     /translation="MSGSSRQNRKRRVFVALDPPVELRSEAEAWARHIARSAKGIRVV
                     PARNSHITLAFLGERDEFEIEEVAGALLETAVPATGLALGAPAWLPKRRPRSLVLDIH
                     DDRGELKELQAGMAGALKSALGWKEDRPYRPHLTCARTGRGFDPSHLRVPVSPSIAFS
                     GQSVTLYASHLGPEGAEYEVIERVDQ"
     gene            complement(2012..3139)
                     /locus_tag="PLJ64_08695"
     CDS             complement(2012..3139)
                     /locus_tag="PLJ64_08695"
                     /inference="COORDINATES: protein motif:HMM:NF015400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HNC93600.1"
                     /translation="MSTQSGPITVYGATGYTGKLVAAELASREVPFIVAGRNREKLEA
                     LADRLDGPGGKPESHAVATTDPRGLKELFARSGAVIACAGPFSLHGEPVLAAAVEAGT
                     HYLDTTGEQPFVRLAFDTYGPKALGKGSAVIPAMGFDYVPGDMLAALTAAALGAGETV
                     DKVRLGYHAPFQPSQGTMRSGIEMIKGGDFEWRERALRPASQAVGRPDFDFGGELGLQ
                     KMMHYPSAEHITVPTHLSPRSVETGLTVASFAPNAASQAVMPAFTRALGLAMRTPLRS
                     LMSKAISAMPEGADDQGRTASRFEIGCEVTSARRMARGRLTGTDVYGLTAALIVDGAI
                     RASRGEVKGSGALAPAVAFDPATFLSGLDRFGFNWEVTPPR"
     gene            3200..4093
                     /locus_tag="PLJ64_08700"
     CDS             3200..4093
                     /locus_tag="PLJ64_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HNC93601.1"
                     /translation="MSFETILYSVEGPVATITLNRPGRLNTIVPPMPDEVEQAVGEAT
                     RDPEVKVIVLRGAGRSFCAGYDFGGGFEHWDEYLTTDGEWDPGKDFALATATELSPTQ
                     KFMSIWRSPKPVIAQVHGYCVGGGSDFALCADLIVASEDAVIGTPYSRMWGAYLSGMW
                     IYRLGLARTKYHALTGRPLNGKQAAEVELINQAVPFEELEATVAGLAADLASIPASQL
                     AAMKLVVNQAYENMGLASTQTLGPILDGLMRNTPEALEFIDKAESEGVGRVIEDRDGG
                     FGDYSQAPPELQPDPRHIIEP"
     gene            4093..5301
                     /locus_tag="PLJ64_08705"
     CDS             4093..5301
                     /locus_tag="PLJ64_08705"
                     /inference="COORDINATES: protein motif:HMM:NF018479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HNC93602.1"
                     /translation="MPASRARRNPDEHYGASNLELFFDLVFVFAITQVSHLLLENLTW
                     RGALEAAMVLLVVWWAWQYTTWATNELDTDRMPVRFVLLVIMMACLLMAVAIPEAFGD
                     KGLLFALSYVAIQIGRQAFLTFSAEPHTIARARGLHILIWFCFAAPFWIVGALVDGDA
                     RIAFWLVALTIDYCGPFVTYYVPWLKKVSPDAWKIGSGHFAERFQLFTIIALGETIVL
                     TGATTAKLEFDLVTALAFMAAFVSTACLWWLYFNYMATILERVLDEAEDRTAIGRDIF
                     TYGHIGIIVGIILCAVGDEIILTHPKEYLHTPELVAVVAGPSLYLLAFVPTRWRLTHG
                     LPWRRVSGAIACIAIGVFAYLVHPPALVTGGLLLLALIGVISHEYFFRWQRSDAEELD
                     LARKLNVETR"
     gene            5368..7377
                     /locus_tag="PLJ64_08710"
     CDS             5368..7377
                     /locus_tag="PLJ64_08710"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HNC93603.1"
                     /translation="MPEPVERRSGQRYMPGLDGLRAIAVLAVIAYHLRVGFAPGGLLG
                     VGVFFTLSGYLITDILLEGWVTRKLSLKNFWLARARRLLPALFTMLAVVLLWVWIGNP
                     DLLGTLRGETISSIFFTENWWAIAQNMSYFDRFGAPSPMSHLWSLAVEEQFYIIWPWL
                     LLLLLKISPAKDHGKRARRERIRPNLALITLGLAVVSAVLMAVLYEPGFDNTRVYEGT
                     DTRAFALLFGAALAMVWPSRRLDRDLPAKARRNMDLLGVGALVVILVLIWQTSELSPF
                     LYRGGMVILALATTVLVAVLAHPATRLGPIIGCRPLRWIGVRSYAIYLWQLPVIILTT
                     PVGSSGFHPLRAPVQVATIFLIAALSWKYIEDPVRKGAVGRLWKRFRAGEFSWKKFGP
                     EGKGFTVAGGVVVLVLLMAMVGVAPSSQLAPIATTGGAGQAEVEAIATAAPVAKTVIS
                     QKDRDEFPKSLCSSVAYIGDSTSLGLYGPEADQYLPDKNDQLPARLDDVGVYEQHIDI
                     SGARSSYEEVEGMPNAVEAAQAIVDAGFDGCWIFAMGTNDTANVAVGSAVGMRERIDS
                     LMGVADGNPALWINVRTLNPPNPAYSEEQMQAWDDELLNACEDYPNMRVYDWANKVKD
                     EWFIDDGIHFTSEGYKVRAQAIANAVPKAFGIWSSFWNQSGCLIE"
BASE COUNT         1214 a         2475 c         2444 g         1356 t
ORIGIN      
        1 acaccttctt ctgccgcatc cacccgctga tgcgcggcgc cttccgggtc aagtagccgt
       61 cgccccgttc ccaatactga ttcggcatcg ttccctctga aatgtctctc ggaggcttca
      121 ttcaacttcc gggtgacgtt tggttggctg tggtgcccca aagtcaccca aatggaggcg
      181 aaacgcgtac gtgatccgct ctgggtgaag tttggtcggc tgtggcgccc caaaagtcac
      241 ccggaatcct tccaactgtg atcaagcgac ccgggccagc gcccggcacc gggcgatctc
      301 ccgaatgacc aaactcgcga cgagttcggg gtgctcggtg atctcctccc aggtgaacct
      361 gatcacctgg tagccgagcc gacggaggtc gttttcccgc gccgcgtcat cgaggaaagc
      421 gaaccgtccg gcgtgccctt cgtacccatc gacctcaacg atcaagccga tgtcatccca
      481 actgaagtca gggaagtatc ggcccttgcg acggttcact cttggagtct cgatgccgta
      541 tctggcgcag agttcgagga atagggtctc gagaagagtc cggacatcct taaggtccgg
      601 gtgtcgccga gcgacgagat tctggaactt tctgattcct tcgagtccgg aacccaactc
      661 aaggcaagct ctcaactctt cttcattgag caagcccagg cgatccgctt ccttgaaggc
      721 atgtccgaac tcatcgtcgg ggagttcccg ggctagatcc agcaggatcc aggaaggtcg
      781 caggatcggg attccgtatt tgcgggtgac ctgagcggga ttgaagtatc gactcctgcg
      841 aaccctcacc ggtctcgcct cggcaacacc tcgacgatcc agaacgaact cgccagatct
      901 gtggctgttg cgccgtttaa cctcaaggtt cccgcggtct tttcgaatgt cccaaacatc
      961 ggcagcagcc cggccggaga ggactgcacc gcccccggcc acaagagtgg cggccatgca
     1021 ggcagccagt cgaccctcgg cgcggcggcc aaccgcaaag acggccctgc ccgccggcag
     1081 aaggcgattc tggtcttgac gatgcttgat catcgccttg gaaaggctcg ctccgagcat
     1141 ctgcgttcgg gtcacaagcc cgaattgctt ccccgcgatc ctctggagtg aggcttcgat
     1201 ttccgctcgg tcagacatcc gtcgagcctg ccacgaaaaa gctcaaaagt gtgcaaactg
     1261 agcaacgatt cacacacatg ccggtctcag atgccggtct cagccaaccg tcgccgagac
     1321 ggaagcgtag gcctgaccga ttactccggg ttgcgtttgg gctgccgggg tgccccaaag
     1381 gtcacccgga aggtgggtag ggcgtcaggc tggccggtcc ggcccgggcc gggtcggctt
     1441 ctactgatcg acccgttcga tcacttcgta ttcggcgccc tcgggcccca agtgggaggc
     1501 gtagagtgtc accgactgac cgctgaaggc gatcgagggg ctgaccggca cccgcagatg
     1561 agagggatcg aagccgcggc cggtcctggc gcaggtgagg tgcgggcggt agggccggtc
     1621 ctccttccag cccagggccg atttcagggc cccggccatt ccggcctgaa gctccttcag
     1681 ttcgccccgg tcatcgtgga tgtccaggac caggctgcgg ggccgccgct tcggcaacca
     1741 ggccggggca ccgagcgcca ggccggtcgc cgggacggcc gtctcgagca gggcacccgc
     1801 gacctcctcg atctcgaatt cgtcacgctc gccgagaaag gcgagggtga tgtgggaatt
     1861 gcgggccggc accaccctga tgcccttggc gctgcgagcg atgtgacggg cccaggcctc
     1921 cgcttccgag cgcaactcga ccggcgggtc aagggccacg aagacccggc gcttgcggtt
     1981 ctgtcgactg ctcccgctca cccgcctgag gttagcgggg cggggttacc tcccagttga
     2041 atccgaagcg gtcgagcccg gaaaggaagg tggccggatc gaaggcgacg gccggggcca
     2101 gggcgccgga gcccttgacc tcgccgcggg atgcccggat cgcgccgtcg acgatcagcg
     2161 ccgcagtgag cccgtaaaca tcggtcccgg tcaggcggcc acgtgccatg cgccgggcgg
     2221 aagtcacttc gcagccgatc tcgaaccggc tggcggtacg gccctggtcg tctgcgccct
     2281 ccggcatcgc cgagatcgcc ttcgacatga gtgagcgcag cggtgtcctc atggccaggc
     2341 cgagcgccct ggtgaaagcc ggcatcaccg cctggctggc ggcattgggc gcgaacgagg
     2401 cgacggtcag gccggtttcg acgctacggg gtgaaaggtg ggtcggcacc gtgatgtgct
     2461 cggccgaggg gtaatgcatc atcttctgga ggccgagctc gccaccgaaa tcgaagtcgg
     2521 gccggcccac agcctgggag gcggggcgta gcgcccgctc gcgccactcg aagtcgccgc
     2581 ccttgatcat ttcgattccc gacctcatcg ttccctggct cggctggaag ggagcgtggt
     2641 aaccgagccg caccttgtcc acggtctcgc cggctccgag cgcggccgcg gtcagggcgg
     2701 caagcatgtc ccccggcaca tagtcgaaac ccatcgccgg aatcaccgcc gaacctttcc
     2761 cgagggcttt cggcccgtag gtgtcgaagg caagccggac gaagggctgc tcgccggtgg
     2821 tgtccagata gtgggtgccg gcctcgactg cggcggccag gaccggctcg ccgtggagcg
     2881 agaacgggcc ggcgcaggcg atcacggcgc cgctccgcgc gaagagctcc ttcaggcccc
     2941 gcgggtcggt ggtcgccacg gcatggctct cgggcttgcc gccggggccg tcgaggcggt
     3001 cggccagggc ctccagcttc tcgcggttgc ggccggcgac gatgaacgga acctcgcgcg
     3061 aggccagctc ggcggcgacc agctttccgg tgtagccggt ggctccgtag acggtgatcg
     3121 gtccggattg ggtgctcatg ccggggattc tcgcaggcag ggtcgggttg ccgcggggaa
     3181 cggcggtagg gtgccacggg tgagtttcga aacgattctc tactcggtcg aggggccggt
     3241 cgcgacgatc acgctgaacc gtcccgggcg gctcaacacg atcgtgccgc cgatgcccga
     3301 tgaagtcgaa caggcggtgg gggaggcgac ccgcgacccg gaggtcaagg tgatcgtgct
     3361 gcgcggggcc ggtcgttctt tttgcgccgg ctacgacttc ggcggcggct tcgagcactg
     3421 ggacgagtac ctgacgaccg atggcgaatg ggatccgggc aaggacttcg ccctggccac
     3481 cgcgacggag ctctcaccga cccagaagtt catgagcatc tggcgcagcc cgaagccggt
     3541 gatcgcccag gttcacggat actgcgtcgg tggcggcagc gatttcgcgc tctgcgcgga
     3601 tctgatcgtg gccagcgagg acgcggtgat cggcaccccc tacagccgga tgtggggcgc
     3661 ctacctttcc ggcatgtgga tttaccggct cggcctcgcc cggaccaagt accacgccct
     3721 gaccggccgg ccgctgaacg gaaagcaggc ggccgaggtc gagctgatca atcaggcggt
     3781 ccccttcgag gagcttgaag cgacggtggc cgggctggcc gccgatctcg cttcgattcc
     3841 ggcctcgcag ctggcggcga tgaagctggt cgtgaaccag gcctacgaga acatgggtct
     3901 cgcctcgacc cagaccctcg ggccgattct cgacgggctg atgcggaaca cgccggaagc
     3961 gctggagttc atcgacaagg cggaatccga aggggtgggg cgggtgatcg aggatcgcga
     4021 cggtggcttc ggcgactact cgcaggcgcc gccggagctc cagcccgatc cccgccacat
     4081 aatcgagccc tgatgccggc aagccgcgcc cgccgcaacc cggacgagca ttacggggca
     4141 tcgaaccttg agctcttctt cgatctcgtc ttcgtcttcg cgatcaccca ggtttcgcat
     4201 ctgctgctgg aaaacctgac ctggcggggt gccctcgagg cggcgatggt gctgctcgtg
     4261 gtctggtggg cctggcagta cacgacctgg gccaccaacg agcttgacac cgaccggatg
     4321 ccggtccggt tcgttctgct ggtgatcatg atggcctgcc tgctgatggc ggtcgccatc
     4381 ccggaggcct tcggcgacaa gggcctgctc ttcgccctct cctatgtggc gatccagatc
     4441 ggccggcagg cattccttac cttcagcgcc gaaccgcaca cgatcgcccg ggcccggggc
     4501 cttcacatcc tgatctggtt ctgctttgcc gcccccttct ggatcgtcgg cgcgctggtc
     4561 gatggcgacg cccggatcgc gttctggctg gtcgccctga cgatcgacta ctgcggaccc
     4621 ttcgtgacct actacgtccc ctggctgaag aaagtaagcc ccgacgcgtg gaagatcggc
     4681 agcgggcact tcgccgagcg gttccagctg ttcacgatca tcgccctggg ggagacgatc
     4741 gttctcaccg gagcgaccac ggcgaaactc gagttcgacc tggtcacggc cctcgcgttc
     4801 atggccgcct tcgtcagtac cgcctgcctg tggtggctct acttcaacta catggcgacc
     4861 atcctcgaac gggtcctcga cgaggcggaa gaccgcaccg cgatcggtcg tgacatcttc
     4921 acctacggcc acatcggaat catcgtcggg atcattctct gcgcggtcgg tgacgagatc
     4981 atcctcaccc atccgaagga gtacctccac acaccggaac tggtcgccgt ggttgcgggc
     5041 ccgtcccttt acctgctggc cttcgtgccg acccggtggc ggctcaccca cgggcttccg
     5101 tggcggcggg tttccggcgc aatcgcctgc atcgcgatcg gggtctttgc ctacctcgtg
     5161 catccgcccg ccctggtaac cggtggcctg cttctgctcg ccctgatcgg ggtgatcagc
     5221 cacgagtact tcttccgctg gcaacgaagc gatgccgagg agcttgacct ggcgcgcaaa
     5281 ctgaacgtcg agacccggtg acagctgcct gaccggcgcc accccgggat ggtggggtgg
     5341 ctcgctggcg ggtagattct ccgggtcatg ccggaaccag tagaaaggcg atccggtcag
     5401 cgctacatgc ctggcctgga cgggctcagg gccattgccg ttctcgcggt gatcgcctac
     5461 cacctccggg tcggattcgc gccgggtggc ctgctcgggg ttggcgtgtt cttcacgctt
     5521 tccggctacc tgatcaccga catcctgctc gagggctggg tgacccggaa gctgagcctg
     5581 aagaacttct ggctggcccg cgcccgccgg ctcctgccgg ccctgttcac gatgctggcc
     5641 gtcgtcctgc tctgggtctg gatcggcaac cccgacctgc tcggcaccct gcgcggcgag
     5701 acgatctcct cgatcttctt caccgagaac tggtgggcga tcgcccagaa catgtcgtac
     5761 tttgaccggt tcggggcgcc ctcgccgatg agccatctct ggtcgctggc ggtcgaggag
     5821 cagttctaca tcatctggcc ctggctgctt ctgctcttgc tgaagatctc gccggccaag
     5881 gaccacggca agcgggcccg ccgcgagcgg atccgtccca acctcgcctt gatcacgctc
     5941 ggtctcgcgg tcgtctcggc ggtgctgatg gccgtgctct acgaaccggg cttcgacaat
     6001 acgcgggttt acgaaggcac cgacacccgc gccttcgcac tgctcttcgg ggccgccctt
     6061 gccatggtct ggcccagccg ccgtctggac cgggacctgc cggccaaggc ccggcgcaac
     6121 atggacctgc tcggggtcgg ggccctggtg gtcatcctcg tcctgatctg gcagacctcc
     6181 gagctctcac ccttcctgta ccgcgggggc atggtcatcc tcgcccttgc caccaccgtt
     6241 ctggttgccg tcctcgcgca tccggcgacc agactcggac cgatcatcgg ttgccgcccg
     6301 ctgcgctgga tcggggttcg ctcctacgcg atctatctct ggcagttgcc cgtgatcatc
     6361 ctgaccaccc cggttggttc cagcggcttt catccgcttc gggctccggt tcaggtcgcg
     6421 accatcttcc tgatcgcggc gctttcctgg aagtacatcg aggacccggt ccgcaaggga
     6481 gcggtcggcc ggctctggaa gcgcttccgg gcgggtgagt tcagctggaa gaagttcggc
     6541 ccggaaggca agggatttac cgtcgccggc ggggtggtcg tcctggtcct gctgatggcc
     6601 atggtcgggg ttgcgccttc cagccagctg gccccgatcg ccacgaccgg cggcgccgga
     6661 caggcggagg tcgaggcgat tgccacggcg gcaccagtcg ccaagaccgt gatcagccag
     6721 aaggaccgcg acgagttccc caagagtctc tgcagctcgg tcgcctacat cggcgattca
     6781 acctcgcttg gcctctacgg accggaggcg gaccagtacc tgcccgacaa gaacgatcag
     6841 ttgccggccc gcctcgacga tgtcggtgtc tacgagcagc acatcgacat ttccggcgcc
     6901 cgctcctcct acgaggaagt tgaaggcatg ccgaacgcgg tcgaagcggc ccaggcgatc
     6961 gtcgacgccg gattcgacgg atgctggatc tttgcgatgg gaaccaatga caccgcgaac
     7021 gtggcggttg gctcggcggt cgggatgcgg gagaggatcg acagcctgat gggcgtggcc
     7081 gacggaaatc cggcgctctg gatcaacgtc cgcaccctga acccgccgaa tccggcctat
     7141 tcggaggagc agatgcaggc ctgggacgac gagttgctga acgcctgcga ggactacccg
     7201 aacatgcgcg tctacgactg ggccaacaag gtcaaggacg agtggttcat cgatgacggc
     7261 atccacttca cctccgaggg ttacaaggtg cgcgcgcagg cgatcgccaa cgcggtcccg
     7321 aaggccttcg gcatctggtc cagcttctgg aatcagtccg gctgtttaat cgagtagcgg
     7381 gcggcgccag gaaccaccag ccgtccgctg gcttcgtcgg cggccggaaa cgacgctcac
     7441 gtacccgcgt actatcgcgg tcatttccgg cctgtctcct tgccagcga
//
LOCUS       DAOQKN010000125         7450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_4854_length_7450_cov_5.309804, whole genome shotgun sequence.
ACCESSION   DAOQKN010000125 DAOQKN010000000
VERSION     DAOQKN010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 7450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7450
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4854_length_7450_cov_5.309804"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            304..927
                     /locus_tag="PLJ64_08715"
     CDS             304..927
                     /locus_tag="PLJ64_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HNC93604.1"
                     /translation="MSQATVTNAASEAAPDSSGAAENAVGARVKALREAMDLSLRDLS
                     ERSGVSPTMLSQVERGSTSPTLAVAEKIASGLDLTLSQLLRLDEVQHVVISRSTDHLV
                     RNRKGHRIEEMTPPLPGQRADVSSHRLEPGASTGGAGDAPMHEPGSRETAVVLRGKVV
                     LVFEGDRHQLTEGDSVTFDADLPHHFENESDEPVEFVAVIAAGLRRG"
     gene            944..2329
                     /gene="leuC"
                     /locus_tag="PLJ64_08720"
     CDS             944..2329
                     /gene="leuC"
                     /locus_tag="PLJ64_08720"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HNC93605.1"
                     /translation="MPKTMFEKIWEAHEVRENLLYIDLHLVHEVTSPQAFEGLRMSGR
                     KLRRPDKTVATADHNVPTDGTPAAAMIKDALSRKQVETLEQNAADFGVPVYSLGSETQ
                     GIVHVIGPELGLTQPGMTIVCGDSHTSTHGAFGALAFGIGTSEVEHVMATQTLVQNKP
                     KTMRINYHGKLGEGVTSKDLILATIGKLSTSGMTGYVVEYAGEAIEALTMEQRMTVCN
                     MTIEGGGKAGMIAPDETTFEYMRGKPGVPGDFDAAVERWKQLPTDEGATFDSEINIDA
                     TEISPMVTWGTTPGMVIQVTDTVPDPEKMDSPADKEAAERALQYMGLEAGTPMEEIRP
                     ERVFIGSCTNSRISDLREAAGAIKGRKVADSVRAMVVPGSALVKAQAEEEGLDEIFRQ
                     AGFEWRGAGCSMCLGMNPDILIEGERCASTSNRNFEGRQGKGGRTHLVSPTMATAAAI
                     EGHFVDIRNWS"
     gene            2339..2902
                     /gene="leuD"
                     /locus_tag="PLJ64_08725"
     CDS             2339..2902
                     /gene="leuD"
                     /locus_tag="PLJ64_08725"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNC93606.1"
                     /translation="MEPISTIEGEIAVLDRSDVDTDQIIPKQFLKRVERTGYGEFLFY
                     DWVRDGEIELEPHPILVSGRNFGSGSSREHAVWALHQFGFQAVIAPSFSDIFYSNSTK
                     NGFLPVELDEEKCQQIVEAGEAKIDLESQTVSWNGGEAGFEIDAEIKRRLLGGLDDIA
                     LTLQSEDEIEAFEAGPGGTYGPVTTSL"
     gene            2958..3746
                     /locus_tag="PLJ64_08730"
     CDS             2958..3746
                     /locus_tag="PLJ64_08730"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNC93607.1"
                     /translation="MSSDQESRPADWEADKYSRTATPQREWSEAVLDRLELSGDETVV
                     DAGCGSGNVTEDLLAKLPDGRVIGVDGSPSMIEEATARLGDDPRTSFICQDLAKLQLD
                     EQVDHVFSNATFHWIADHDALFRSLAGATRPGGSLIAQCGGRGNVAEVIDALGVVNRQ
                     APFAEIFGEYDDPWNFAGPEETEIRLIDAGYEDVECWLEERVAHPEDPRGFFEASFLA
                     PARQVLDDEQFTEYTDRLLAEMGQPASFNYVRLNMTAKRKNENE"
     gene            3739..4827
                     /gene="leuB"
                     /locus_tag="PLJ64_08735"
     CDS             3739..4827
                     /gene="leuB"
                     /locus_tag="PLJ64_08735"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HNC93608.1"
                     /translation="MSETRKIVVLAGDGIGPEIVGATRRVLDAVGSFEYEEHLIGGAS
                     IDEHGVALTDEVIEACKASDAVLLGAVGGPKWDEAVAKDPTAPRPEQGLLGIRKALGL
                     YANLRPVKPSPALIDASPLKKELIDGTDLLVVRELTGGIYFGDSGRRDDGAVAYDECA
                     YSVPEIQRIARAAFEAARQRSDSPRVLSVDKANVLETSRLWRETVKQVATEYPDVELD
                     HLLVDFAAMKLVSAPAEIDVIVTENLFGDILSDEAAMLTGSLGMLPSASIGAEGQPGL
                     FEPVHGSAPDIAGQGVANPLATMLSAAMMLRYGLGDGAGADRIDDAVDQVLAKGLRTP
                     DIFDGGEGETRVGTEEVTAAVLAELGQG"
     gene            4895..5839
                     /locus_tag="PLJ64_08740"
     CDS             4895..5839
                     /locus_tag="PLJ64_08740"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HNC93609.1"
                     /translation="MEKAEYIWHSGKFVPWDEAQVHVLSHGLHYGTGVFEGVRCYDGE
                     KGPAIFRHKEHLQRFEDSARMYFLDLPFTKEEIGEATRELIRKNGLRDCYIRPLAFRG
                     YGEMGLYAKSAPVEVIIAVWPWGAYLGEDGKKHGIRAKVSSWRRVSSASLIPHAKASG
                     QYLNSILAKTESAKAGYEEAILLDERGMVCEGSGENIFMVKEGKVFTPPQVASILDGI
                     SRKSVMQILEDRGYPVIERDIARSELYLADEIFMCGTAAEVVPVREVDDHPLGDPGEV
                     TRLVQQGYEDAIYGRSPEYSEWLDLVGEPATKTEPSTV"
     gene            5995..>7450
                     /gene="cimA"
                     /locus_tag="PLJ64_08745"
     CDS             5995..>7450
                     /gene="cimA"
                     /locus_tag="PLJ64_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HNC93610.1"
                     /translation="MTAVKLYDTTLRDGMQGQGMSLSAAEKVRVVHALDQLGVQFIEA
                     GFPASNPKEAELFELLAGEKLEQAAICAFGMTRRRGVEADQDEALQILAATFAPVITL
                     VGKTWALHLEKVTKVSREENLGMISESVAYLVGEGKRVMYDAEHFFDAWADDPDYSLE
                     CLKAARDAGAEVLVLCDTNGANLPSQIRPAVEAVKEALPEAELGIHCHNDLECGVANA
                     LAAVTAGARQVQGTINGYGERCGNSNLISILPALQLKLGYEVVPPGKMKQLSGVSHFV
                     ADLLNVPVDPDQAYVGRNAFAHKGGMHVAGIAADARTFEHMDPGAVGNSRKVLASELS
                     GKATVLARAEEIGLEFDDEAARKAVEELKQKEHDGYHYEAAEASFDLLLRREAGQYKP
                     LFTFESFKVLTELRAGEKVETEAIVKIKVGDERYVEVAEGNGPVNALDKALRQAITRH
                     YPELKKIELTGYRVRILDTAHGTGAGTRVLLDSKR"
BASE COUNT         1463 a         2320 c         2462 g         1205 t
ORIGIN      
        1 tcagcgtcaa cgctccggtc gaccacaaca agccgaagca gccccgggac gaccgttaga
       61 cgcacaggga ttcaggatcc cgaacgctta gaaagccccg ccacacggcg gggctttcct
      121 gttgcagggt tgatctgact gcgccggggg ttgatccgac ggcgccgcgg gttgatctga
      181 cggtcgccgc ggggttgatc tgacggtcgc cgcggggctg atccgactgc ggcgcagggc
      241 cggtccgggc cggccgggga ttggacttga caggacgggt gtgcgtgata acgtacaaac
      301 gtaatgagtc aggcgactgt cacaaatgcg gcttcggaag cggccccgga ttcctccggt
      361 gcggcggaga acgccgtggg cgcccgggtc aaggccctgc gggaagcgat ggacctttcg
      421 ctgcgggacc tgtcggagcg aagcggggtc agcccgacca tgctctccca ggtcgagcgg
      481 ggcagcacca gcccgaccct ggcggtggcc gagaagatcg cctccgggct cgacctgacc
      541 ctttcgcagc tgttgcgtct ggacgaggtc cagcatgtcg tgatcagccg ctcgaccgac
      601 cacctggttc gcaaccgcaa gggtcaccgc atcgaggaga tgacgccgcc cctgcccggg
      661 cagagggccg acgtttcctc gcacaggctc gagccggggg ccagtacggg cggggccggt
      721 gacgcgccga tgcatgagcc cggcagccgc gagaccgcgg tggtcctgag gggaaaggtc
      781 gtcctcgtat tcgaaggcga ccgccaccag ctgaccgaag gtgacagcgt caccttcgat
      841 gccgatttgc cacaccattt tgaaaacgaa agcgatgaac ccgtcgagtt cgtcgctgta
      901 attgccgccg gtctgcgccg gggctgatcg ggaaggagat cagatgccaa agacgatgtt
      961 cgaaaagatc tgggaagccc acgaggtccg ggagaacctg ctctacatcg atctccacct
     1021 cgtccacgag gtgacctcgc cacaggcctt cgagggtctg cgcatgtcgg gccgcaagct
     1081 gcgccgcccc gacaagacgg tcgccaccgc cgaccacaac gtgccgaccg acggcacgcc
     1141 ggccgcggcg atgatcaagg acgcgctctc gcgcaagcag gtcgaaaccc tggagcagaa
     1201 cgccgccgac ttcggcgtgc cggtttacag cctcggttcc gagacccagg gcatcgtcca
     1261 cgtgatcggc ccggagctgg gactgaccca gcccggcatg accatcgtct gtggcgactc
     1321 ccacacctcc acccacgggg cgttcggggc gttggccttc ggcatcggca cctctgaggt
     1381 ggagcacgtg atggccaccc agaccctggt ccagaacaag ccgaagacga tgcggatcaa
     1441 ttaccacggc aagctcggcg aaggcgtgac ttccaaggac ctgatcctcg ccacaatcgg
     1501 caagctcagc acttccggga tgaccggtta cgtggtcgag tacgccggcg aggcgatcga
     1561 ggcactgacc atggagcagc ggatgaccgt ctgcaacatg acgatcgagg gcggcggaaa
     1621 ggccggcatg atcgcccctg acgagacgac cttcgagtac atgcggggca agccgggcgt
     1681 gccaggggac ttcgatgcgg cggtcgagcg ctggaagcag ctgccgaccg acgagggagc
     1741 gaccttcgat tccgagatca acatcgacgc gaccgaaatc tcgccgatgg tcacctgggg
     1801 cacgaccccg ggcatggtga tccaggtcac cgacacggtg ccggacccgg agaagatgga
     1861 ctcgcccgcg gacaaggaag cggccgagcg ggcgctgcag tacatgggcc tcgaagccgg
     1921 aactccgatg gaggaaattc gcccggagcg ggtcttcatc ggctcctgca ccaactcgcg
     1981 aatctcggac ctgcgggaag cagccggcgc gatcaagggc cgcaaggtgg ccgacagtgt
     2041 gcgggcgatg gtcgtcccgg gctcggccct ggtcaaggcc caggccgaag aggaaggtct
     2101 cgacgagatc ttccgccagg ccggattcga atggcgcggc gccggctgtt cgatgtgcct
     2161 cggcatgaac ccggacatcc tgatcgaggg cgaacgttgc gcctcgactt cgaaccgcaa
     2221 cttcgaaggc cgccagggca agggcggccg gacccacctc gtcagcccga ccatggccac
     2281 cgcggcagcc atcgaaggcc acttcgtcga catccgcaac tggagctagg agctagcaat
     2341 ggaaccgatc agcacaatcg aaggcgaaat cgccgttctc gaccgcagcg acgtcgacac
     2401 cgaccagatc atccccaagc agttcctgaa gcgggtagag cggaccggtt acggcgagtt
     2461 cctcttctac gactgggtgc gcgacggcga gatcgaactc gagccgcatc cgatcctggt
     2521 ttccggccgg aacttcggct cgggatcctc gcgcgagcac gccgtctggg cgctccatca
     2581 gttcggcttc caggccgtga tcgccccgtc cttctcggac atcttctatt cgaacagcac
     2641 caagaacggc tttctgccgg tggagctgga cgaggagaaa tgccagcaga tcgtggaggc
     2701 cggcgaggcg aagatcgacc tcgagagcca gaccgtgagc tggaacgggg gagaggccgg
     2761 gttcgagatc gacgccgaga tcaagcggcg cctgctcggc gggctcgacg acatcgccct
     2821 cacccttcag tccgaggacg agatcgaggc cttcgaggcc ggccccggcg ggacctacgg
     2881 gcccgtgaca acatcccttt agggcgcgcg tcgagcgaac tgattcagcg cgcctgtctt
     2941 tgtagaggag atcaatcatg agcagtgatc aggaatcccg tccggcggac tgggaggcag
     3001 acaagtattc ccgcacggcg actccgcagc gcgaatggtc agaagccgtg ctcgaccgtc
     3061 tggaactgag tggcgacgaa acggtggtcg atgccggctg tggttccggc aacgtgaccg
     3121 aggacctgct ggcgaagctc cccgacggcc gggtgattgg cgttgacggt tccccgagca
     3181 tgatcgagga agccaccgcg cgcctcgggg acgacccgcg gaccagcttc atctgccagg
     3241 acctggcgaa gctccagctt gacgagcagg tcgaccatgt cttctcgaac gccaccttcc
     3301 actggatcgc tgaccatgac gccctgttcc ggtcgctggc cggggcgacc cggcccggcg
     3361 gcagcctgat cgcgcaatgc ggcggccgcg gcaacgtggc cgaggtgatc gacgcgctcg
     3421 gggtcgtcaa ccggcaggca cccttcgcgg agatcttcgg tgagtacgac gacccgtgga
     3481 acttcgccgg gccggaagag accgagatcc ggctgatcga tgccggttac gaggacgtgg
     3541 agtgctggct ggaggaacgg gtcgcccatc cggaggatcc gcggggattc ttcgaagctt
     3601 cgtttctggc cccggcccgg caggtcctcg acgatgaaca gttcaccgag tacacagacc
     3661 gcttgctggc cgagatgggt caaccagcca gcttcaacta tgttcgcctg aacatgaccg
     3721 cgaaaaggaa gaacgagaat gagtgagacc cggaagattg tggtgctggc cggcgacggt
     3781 atcggcccgg aaatcgtcgg cgcgacccgt cgggtcctcg acgcggttgg cagcttcgaa
     3841 tacgaggaac acctgatcgg tggtgcctcg atcgacgagc acggggtcgc cctgaccgac
     3901 gaggtgatcg aagcctgcaa ggcatccgac gcggtgctgc tcggcgcagt cgggggcccg
     3961 aagtgggacg aggccgtggc caaggacccg accgcgccgc gcccggaaca ggggctgctc
     4021 ggcattcgca aggcgctcgg cctctacgcg aacctgcggc cggtcaagcc ttccccggcg
     4081 ctgattgatg ccagcccgct gaagaaggag ctgatcgacg gcaccgacct gctggtcgtc
     4141 cgggagctca ccggcggcat ctacttcggt gattccggcc gccgtgatga cggtgccgtc
     4201 gcctatgacg agtgcgccta ctcggtgccg gagatccagc ggatcgcccg ggccgccttc
     4261 gaggctgccc gccagcgctc ggacagtccc cgggtgcttt cggttgacaa ggcgaacgtg
     4321 ctcgagactt cgaggctctg gcgggagacc gtcaagcagg tcgccacgga atatcccgat
     4381 gtcgagctcg atcacctgct agtcgatttc gcggcgatga agctggtttc cgctccggcc
     4441 gagatcgacg tgatcgtcac cgagaacctt ttcggagaca tcctcagcga cgaggcggcc
     4501 atgctgaccg gttcgctggg catgctgccc tcggcctcga tcggggccga aggccagccg
     4561 gggctctttg aaccggttca cggatcggcc ccggacatcg ccggccaggg ggtggcaaac
     4621 ccgctcgcga cgatgctttc cgccgcgatg atgctgcgct acggcctcgg cgacggcgcc
     4681 ggggcagacc ggatcgacga cgcggtcgac caggtcctcg caaagggcct gaggacgccg
     4741 gacatctttg acggtggcga gggggaaacc cgcgtcggca ccgaagaagt cacggcagcg
     4801 gttctggccg agctgggtca gggctagaat catctgagtg cgcccgaagg atctggcgta
     4861 ccatcaccgg gaaaagcaac gcgaaggaga agcagtggaa aaggctgagt acatctggca
     4921 cagcggcaaa ttcgtcccct gggacgaggc gcaggtgcat gtactgagcc acgggctcca
     4981 ctacggaacc ggagtcttcg aaggcgtgcg ctgctacgac ggcgagaagg gcccggcgat
     5041 tttccgccac aaggagcacc tccagcgctt cgaggattcg gcccgcatgt acttcctcga
     5101 cctgcctttc accaaggaag aaataggcga ggcgacgcgc gagctgatcc gcaagaacgg
     5161 gttgcgtgac tgctatatcc gcccactggc ttttcgcggc tacggcgaga tgggtctcta
     5221 cgcaaagtca gctcccgtcg aggtcatcat cgcggtctgg ccctgggggg catacctcgg
     5281 cgaggacggc aagaaacacg gcatccgggc caaggtttct tcctggcgcc gtgtttcctc
     5341 ggccagcttg atcccgcatg cgaaggcttc cgggcagtac ctgaactcga tcctggccaa
     5401 gaccgaatca gccaaagccg gttacgaaga agcgatcctg cttgacgaac ggggaatggt
     5461 ctgtgaaggc tccggcgaaa acatcttcat ggtcaaggaa ggcaaagtct tcacaccgcc
     5521 acaggttgct tcgattctcg acgggattag ccgcaagtcg gtcatgcaga tcctcgagga
     5581 caggggatac cccgtgatcg agcgggacat cgcccgttcc gagctttacc tggccgatga
     5641 aatattcatg tgcggcaccg ccgccgaagt ggttccggtc cgggaagtcg atgatcatcc
     5701 gctcggtgat cccggtgaag tgacccggct cgtccagcaa ggctacgaag atgcgatcta
     5761 cgggcgctca cccgagtatt cggaatggct ggatctggtc ggcgagccgg caaccaagac
     5821 cgaaccaagt acagtctgag atcaatgctt cacgccatca ccgaaatcag gactacccga
     5881 attacggggg ctgcggggcg ctgaccctgc ggcagcgcgg cgggacgccg tgtgcagtcc
     5941 tcccggaccc gaccatggtt ccgggcctcc tgatttcctg aaaggcagtt tccgatgaca
     6001 gcagtgaagc tctatgacac gactctccgc gacggcatgc agggtcaggg catgtccctg
     6061 tccgctgctg aaaaggtgcg tgtggttcat gccctcgatc agctcggagt tcagttcatc
     6121 gaggcaggct ttccggcttc aaacccaaaa gaggccgagc tcttcgagct gctcgccggg
     6181 gaaaaactcg agcaggccgc gatctgcgcc ttcgggatga cccgccgacg cggggtggag
     6241 gctgaccagg atgaggccct gcagatcctc gcggcgactt tcgccccggt catcaccctg
     6301 gtcggcaaga cctgggctct tcatctggaa aaagtgacca aggtctcccg cgaagaaaac
     6361 ctggggatga tcagtgaatc ggtcgcctac ctggtcggag agggcaagcg agtcatgtac
     6421 gacgccgaac acttcttcga cgcctgggcc gatgaccctg actattcact cgaatgcctg
     6481 aaggctgccc gtgatgccgg cgccgaggtg ctggtgctat gcgacaccaa cggggccaac
     6541 ctgcccagcc agatcaggcc cgcggtcgag gctgtgaagg aggctctgcc cgaggccgaa
     6601 ctcggtattc attgccacaa tgacctcgaa tgcggagtcg ccaatgcact ggccgcggtt
     6661 accgcagggg cgaggcaggt ccagggcacg atcaacggct atggagagcg ctgcggcaac
     6721 tcgaacctga tctcgatcct gcccgcactt cagctcaagc tggggtatga agttgtgccg
     6781 cccgggaaga tgaagcaact ctcaggtgtc tctcactttg tggccgactt gctcaatgtt
     6841 ccggtagatc ccgaccaggc atatgtaggt cgcaacgcct ttgcgcacaa aggcggcatg
     6901 catgtcgccg ggatcgcggc cgatgcccgg accttcgagc acatggatcc gggcgcggtc
     6961 gggaattccc gcaaggttct cgcctccgaa ctttccggca aggcgactgt gctggcccgg
     7021 gccgaagaaa tcggacttga gttcgacgac gaggcagccc gcaaggcggt cgaggaactg
     7081 aaacagaagg aacacgacgg ctatcactac gaagcagcgg aggcttcctt cgacctgttg
     7141 ctacgccgcg aggccgggca gtacaagccg ctcttcacct tcgagtcctt caaggtcctg
     7201 acggaattgc gtgccggcga gaaggtcgag accgaggcga tagtgaaaat caaggtcggt
     7261 gatgagcgct acgtggaggt ggccgaagga aatggtccgg tcaacgcgct ggacaaggct
     7321 ctacgtcagg cgatcacgcg ccactacccc gaactgaaga agatcgaact gaccggatac
     7381 cgggtaagga tcctcgacac ggcccacggt accggcgccg gcacccgcgt cttgctcgat
     7441 tcgaagcggg
//
LOCUS       DAOQKN010000126         7438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_4871_length_7438_cov_5.477990, whole genome shotgun sequence.
ACCESSION   DAOQKN010000126 DAOQKN010000000
VERSION     DAOQKN010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 7438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7438
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4871_length_7438_cov_5.477990"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="PLJ64_08750"
     CDS             <1..611
                     /locus_tag="PLJ64_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93611.1"
                     /translation="ERRLFNLTASRWELSDLGYAPVPQPERIVSAVLDTPFGPCELHN
                     VHVPPAPSQGLAKLETLERLHDWLAVPGSHHRILCGDLNVPRLEYPDGVIETFASNHP
                     PEQFERWDRAEKLLLTGLAEYGLKDVFRSLHGYESPEPSWFPHRRNPGPGLPGHRLDH
                     VLASDSLNAVWAGYQHGWREAGLSDHSALEVIFEPRAGNLEN"
     gene            complement(600..1448)
                     /locus_tag="PLJ64_08755"
     CDS             complement(600..1448)
                     /locus_tag="PLJ64_08755"
                     /inference="COORDINATES: protein motif:HMM:NF012490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HNC93612.1"
                     /translation="MTGDEIQLNDPQTLYERWEGAQWNPFTVPLGLDQEQWSGLGETD
                     RGLIYWVLSSLMVAEERITTKFSGLVGAHGSEEEASFLATQQVDEARHMQFYARFQNE
                     VIADPATVAAHVDRSREQVSPAFEQIFDVELVQAHEHLIANPGDLAAKVQFVTLYHLI
                     LESTLGLTTFKFVTDYLKEHELLPGFVDGYSKIHHDETRHIGYGVWFLRESVRESPEV
                     APAAVREMLRKLLPSVAESLSPSSEPGGPDIEALGVDDEQIREFALGGLTRRLEIIGV
                     PLESVF"
     gene            complement(1445..2323)
                     /gene="arsM"
                     /locus_tag="PLJ64_08760"
     CDS             complement(1445..2323)
                     /gene="arsM"
                     /locus_tag="PLJ64_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HNC93613.1"
                     /translation="MAELEKTTDCCSPEDQATCCEPEAKSECCGEGPECGCPDGAERT
                     DSELREIVRERYAAAARDAGSRDASCCGNDAVITEDQRAVFGSSLYEDHDRSELPEAA
                     QLASLGCGNPTAIADLQEGQTVLDLGSGGGIDVLLSARRVGPAGKAFGLDMTDEMLEL
                     ARSNQAEAGVENVEFLKGEIEAIPLPDDSVDVVISNCVINLSGDKPRVMREAARVLRP
                     GGRFAVSDVIADPDMSEETREDMKQWTGCIAGALTRDEFESNLLAAGFENVEIEETHR
                     VHDQAWSAIVRATMPS"
     gene            complement(2311..2664)
                     /locus_tag="PLJ64_08765"
     CDS             complement(2311..2664)
                     /locus_tag="PLJ64_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNC93614.1"
                     /translation="MSPELTLTPKQKSPNGQPCCEPVVYPDVDRARAERMAKVAKALG
                     DPIRLTLVDVLRKHAGKVCVCELVPLFDVNQSTLSHHLKKLRDAGIVDSERQGLWSYY
                     YVNPEELEELSAWLS"
     gene            2755..3150
                     /locus_tag="PLJ64_08770"
     CDS             2755..3150
                     /locus_tag="PLJ64_08770"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNC93615.1"
                     /translation="MLSLHHTHLMASDIDETIRFWAEGFGGEVVFDREFAGARNVFMT
                     VGKGRIHLYDQAPKVSGQAVVHHLGVQTDEIETVVARLRSMDISVTDVRTEPDASYAM
                     ARGPDDLLVEIFQPDPEAVPEELRPYFGL"
     gene            3214..4332
                     /locus_tag="PLJ64_08775"
     CDS             3214..4332
                     /locus_tag="PLJ64_08775"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNC93616.1"
                     /translation="MPINGRSLRSTVTSDGALELRLIEEEIEEPTGSQVLVSVEATPV
                     NPSDLGVLIGPADASTLGPAEGGGLNGKVPEQALPIMRDRLDKPLPVGNEGAGTVVAA
                     GPEATDLVGRTVSLVGGAMWSDYRLVDGAAVIPLPDDATPADGAALFVNPMTALSMVE
                     MMRAEGHTALVHTAAASNLGQMLVKICKDDEVGLVNIVRKPEQAATLEALGAEHVVDS
                     SLPDFDQRLVEAISATGATLAFDAVGGGTLASDILSAMERSQPPAEFWTPYGSAVHKQ
                     VYTYGSLDFGPTIIQRTFGMRWSVGGYLLTDALGRLGGETVMAMRDRVVSEIKTTFAS
                     SYAETIGLADILDPVHLAAAGKRGTGAKLLIDPSSDRP"
     gene            4382..5353
                     /locus_tag="PLJ64_08780"
     CDS             4382..5353
                     /locus_tag="PLJ64_08780"
                     /inference="COORDINATES: protein motif:HMM:NF014212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93617.1"
                     /translation="MQTVQTVTGEIAADELGRTLIHEHFYSSDEAVSVQWPHVRDREA
                     EHGLAVESAEAVKAHGVRTVVEPTAMLLGRDIQASRELAEETGLQIIVCTGIYTYDHL
                     PQFLLNRDADYIASLFVHDIEEGIQNTGVKAAFIKCAADEPGLTPNIEKIHRAAARAS
                     LQTGAPIMAHSRPASGTGPKQVEVLLEEGVPAERIQIAHTGDTDDLGYIEGLLDKGVY
                     IGMDRYGLDLFLPTAQRNATVIELLNRGYADRMFLSQDFDIPIANGLDWYPLEVLEQL
                     QAAGAATDWSMTFIFEQVIPALREVGMTGDQLDTMLVENPKRWLGRG"
     gene            complement(5367..5822)
                     /locus_tag="PLJ64_08785"
     CDS             complement(5367..5822)
                     /locus_tag="PLJ64_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93618.1"
                     /translation="MPSGGPDFYALMPLSGTLASPQQMQEPFSMNDTLTVMQVIPRDG
                     VVSSINGWLYTMSTLNLVGTTISLTGGLYTSEDGFAEPIQQPATLCTASPALTGVVPP
                     GQISTFACDNIGLDVSAGEVGFFAVEANATGVSLNNSLSLRGSMSMVVD"
     gene            complement(6049..6999)
                     /locus_tag="PLJ64_08790"
     CDS             complement(6049..6999)
                     /locus_tag="PLJ64_08790"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HNC93619.1"
                     /translation="MAKILENMEIGHNVMHGQWDWMRDPKIHSTSWEWDNASPADLWK
                     HSHNEIHHTYTNVIGKDHDLGYGIMRVDEDQRWVPFYLGQPAWNLINAFLFEYSIAAY
                     DLQLKKNWKGRRDNPEFQAKAKQVIRKIRAQMTKDYLVHPALSGPSAPTTFAANLTAN
                     LVRNLWTHSVIMCGHFPEGVATFEKASIEGETRADWYVRQMLGAANIKGSPAMHVMTG
                     NLSFQIEHHLFPDLPSNRYQEISPKVREIFDRYDLPYAEGSLPRQVASAWKKVIRLSL
                     PNDSVGKVKSFLGFGRKEKGPSENVEALPFADDRFDTMAA"
     gene            complement(7020..7241)
                     /locus_tag="PLJ64_08795"
     CDS             complement(7020..7241)
                     /locus_tag="PLJ64_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93620.1"
                     /translation="MTAIQKDPENPIGHLSREDIEQIGRELDAIRQEVLDTRGEDDAA
                     YIRGMIDTQRKLELGSRGFCSSRSFRRPG"
     gene            complement(7249..>7438)
                     /locus_tag="PLJ64_08800"
     CDS             complement(7249..>7438)
                     /locus_tag="PLJ64_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HNC93621.1"
                     /translation="EQAGVLMPSGCRMGICFGCVSRLREGAVRDLRTGEVTTASAGDD
                     IRIQTCVSAAAGTCEIEL"
BASE COUNT         1321 a         2433 c         2330 g         1354 t
ORIGIN      
        1 cggaacggcg actattcaac ctgaccgcct cgcggtggga gttgagcgac ctcggatacg
       61 cgcccgtccc ccagcctgag cgcatcgtct cggccgtcct ggacacaccc ttcgggccct
      121 gcgagcttca caacgttcac gtccctcccg ctcccagcca gggtctcgcg aagctggaga
      181 cgctcgagcg gcttcacgac tggctggcgg tgccgggttc ccatcacaga atcctctgcg
      241 gagacctgaa cgttccccgg ctggaatatc cggacggagt gatcgagacc ttcgccagta
      301 accaccctcc cgaacaattc gagcgctggg accgggccga gaagcttctg ttgaccgggc
      361 tcgccgaata cggactgaag gatgtgtttc gaagcctgca cggctacgaa tcacccgagc
      421 ccagctggtt cccccaccgg cgaaacccgg gaccgggact gcccggccac cggctcgatc
      481 acgtcctggc gtcagactct ctgaacgccg tctgggccgg gtaccagcat ggttggcggg
      541 aggctggcct tagcgaccat tcggccctgg aagtgatctt cgagccccgt gcggggaacc
      601 tagaaaactg attcgagcgg tacgccgatg atttcaaggc gcctggtcag cccgcccaat
      661 gcgaactccc tgatctgctc gtcgtcgacc cccagtgcct cgatgtccgg gccgccgggt
      721 tccgaggacg gcgagagcga ctcggcgacc gacggaagca gcttcctcag catctcccgg
      781 acggcggctg gtgcgacttc aggcgactcc cgaaccgact cgcgaaggaa ccagacccca
      841 tacccgatgt gccgggtttc gtcgtggtgg atcttcgagt atccgtcgac gaaaccaggg
      901 agcagttcgt gctccttcag gtagtcggtc acgaacttga aagtggtcag accgagcgtg
      961 ctctccagaa tcaggtgata gagcgtcacg aactgcacct tcgcggccag atcgccgggg
     1021 ttggcgatca gatgctcatg ggcctggacc agttcgacgt cgaagatctg ttcgaaggcg
     1081 ggtgagacct gctcccggga acgatccaca tgtgctgcca ctgtcgcggg atccgcgatg
     1141 acctcgttct ggaaccgggc atagaactgc atgtgtcggg cctcgtcaac ctgctgggtg
     1201 gcgaggaagc tcgcctcctc ctcggagccg tgggcgccga ccaggccgga gaacttcgtg
     1261 gtgattcgtt cctcggcgac catcagggaa gacagcaccc agtagatcag tccccgatcg
     1321 gtctcaccca gtccggacca ctgttcctgg tcaagcccga gcgggacggt gaagggattc
     1381 cattgcgcac cctcccagcg ttcgtagagg gtctgggggt cgttgagttg aatctcgtcg
     1441 ccggtcacga tggcatcgtc gcacgcacga tcgccgacca tgcctggtcg tggacccggt
     1501 gcgtctcttc gatctcgacg ttctcgaagc ccgccgccag caggttggac tcgaactcgt
     1561 cccgggtcag ggccccggcg atgcagccgg tccattgctt catgtcctcg cgggtttcct
     1621 cggacatatc cgggtcggcg attacgtccg agaccgcgaa ccggcctccc ggacgaagga
     1681 cccttgccgc ttcgcgcata acccggggct tgtcaccgga gaggttgatt acgcaattgg
     1741 agatcaccac gtcaaccgaa tcgtcaggaa ggggaatcgc ttcgatctcc cccttcagga
     1801 actcgacgtt ctcgacgccc gcttcggcct ggttgcttct tgccagctcc agcatctcgt
     1861 cggtcatgtc gagcccgaaa gccttgccgg ccgggcccac cctcctggcc gaaagaagga
     1921 cgtctatgcc gccgcctgat ccgagatcca ggaccgtctg gccctcctgg agatcggcga
     1981 tggcagtcgg gtttccgcag ccgagtgacg ccagctgtgc ggcctcgggc agctcgctgc
     2041 ggtcatgatc ctcgtaaagg ctcgaaccga acaccgcgcg ctggtcctcg gtaatcacgg
     2101 catcgttgcc gcagcaggat gcgtccctgg aacctgcgtc cctggccgcg gcggcatatc
     2161 gctcacggac aatctcccgg agttccgaat ctgttctttc ggcgccgtcc gggcacccgc
     2221 attccggtcc ctcgccgcaa cattcgctct tggcttcggg ttcgcagcag gtggcctggt
     2281 cttcaggcga acagcagtcg gtggtcttct ctagctcagc catgcgctca gttcctctag
     2341 ttcctcgggg ttgacgtagt agtaggacca caggccctga cgttccgaat caacgatgcc
     2401 ggcgtccctg agcttcttca agtgatggga aagcgtcgac tggttgacat caaacaacgg
     2461 cacgagttcg cagacgcaga ccttgccggc gtgcttgcga aggacgtcga ccagggtcaa
     2521 ccggatcggg tcccccagcg ccttcgcgac cttcgccatc cgttccgccc gggcccggtc
     2581 aacatccgga tagacgaccg gctcgcaaca tggctggcca ttcgggctct tctgcttcgg
     2641 ggtcagggtt agttcggggc tcatcgaagc gcattagatc agttccattg atatttgtca
     2701 atagagccga tgaacttcga tggaaagaag acgcgtctag tcttaactca cctgatgctc
     2761 tccctccacc acacccacct gatggcctcc gacatcgacg agacgatccg cttctgggcc
     2821 gaaggattcg ggggcgaggt cgtcttcgac cgcgagttcg ccggcgccag gaacgtcttc
     2881 atgactgtcg gcaagggccg gatccacctt tatgaccagg cgccgaaggt ttccggccag
     2941 gcggtggtcc atcaccttgg cgtccagacc gacgaaatcg aaaccgttgt ggccagactc
     3001 cgcagcatgg acatctcggt aaccgatgtc cggaccgagc ccgacgcctc atacgcaatg
     3061 gcccggggtc cggacgacct gctggtggag attttccagc ccgacccgga agcggtcccc
     3121 gaagaactcc gcccctactt cggcctgtaa acgccttggg ctcccgtcgg ccattcgtgg
     3181 acgggggagg tcaaattggt cacaatggcg tccatgccca tcaacggacg gagtttgcgg
     3241 tcgacagtca cttctgacgg agccctcgag ctccggttga tcgaggagga gatcgaagag
     3301 ccgaccgggt cccaggtgct ggtctcagtc gaggcgacgc cggtgaaccc ttccgacctg
     3361 ggtgttctga tcggtccggc cgatgcctcg acgctcggcc cggccgaagg cggcggcctg
     3421 aacggcaagg tgccggaaca ggccctgccg atcatgcgcg accggctcga caaaccgttg
     3481 ccggtcggca acgaaggtgc cggcacggtc gttgccgccg ggcccgaggc gaccgatctc
     3541 gtgggacgga cggtctccct cgtcggcgga gcgatgtggt ccgattaccg gctggtcgac
     3601 ggggccgcgg tcatccccct gcccgacgac gccacccccg ccgacggcgc cgccctcttc
     3661 gtgaacccga tgaccgcgct ttccatggtc gagatgatgc gggccgaggg ccacaccgcg
     3721 ctcgtacaca ctgccgccgc ctcgaacctc ggccagatgc tggtcaagat ctgcaaggac
     3781 gacgaggtcg gactggtcaa catcgtgcgc aagcccgagc aggctgccac cctcgaagcg
     3841 ctgggcgccg aacatgtggt cgactcctcc ctgcccgact ttgaccaacg gctggtcgaa
     3901 gccatttcgg cgaccggggc taccctggcc ttcgacgcgg tcggtggcgg aaccctggcc
     3961 agcgacatcc tttcggcaat ggaacgcagc cagccgccgg ctgaattctg gaccccatac
     4021 ggatcggccg tccacaagca ggtctacacc tacggctcgc tcgacttcgg tccgaccatc
     4081 atccagcgca ccttcggcat gcgctggtcg gtcggcggct atctgctgac cgacgcgctc
     4141 gggcggctcg gtggcgagac ggtgatggca atgcgcgatc gggtcgtttc ggagatcaag
     4201 accacctttg cctcctcata cgcggagacg atcgggctcg ccgacattct cgatccggtt
     4261 catctcgccg cagccggcaa gcgcggcacc ggcgccaagc tgctgatcga cccatccagc
     4321 gaccgcccct gaccggggcg ggagaaccca caggcaaccg ccgaaagcga agaggagagg
     4381 catgcagacc gttcagactg tcaccggtga gatcgcagcc gacgagctcg gccggacgct
     4441 catccacgag cacttctact catcggacga ggcggtttcc gtccagtggc cacatgttcg
     4501 ggaccgggaa gccgaacacg ggctggccgt tgaatcagcc gaggcggtca aggcccacgg
     4561 cgtgagaacg gtcgtcgagc cgaccgccat gctgctcggt cgcgacatcc aggcgagccg
     4621 ggaactcgct gaggaaaccg gtctccagat catcgtctgc accgggatct acacctacga
     4681 ccatctcccc cagttcctgc tcaaccgcga tgccgattac atcgcctcgc tctttgtcca
     4741 tgacatcgag gaaggtatcc agaacaccgg agtcaaggcc gctttcatca agtgcgccgc
     4801 cgacgaaccc ggcctcaccc ccaacatcga gaagatccac cgggcggcag cccgggcaag
     4861 cctccagacc ggcgccccga tcatggccca cagccgcccc gcttccggga ccggtccgaa
     4921 gcaggtcgag gtccttctgg aggaaggagt gccggccgag cgaatccaga ttgcccacac
     4981 cggtgacacc gacgacctgg gctacatcga gggcctgctg gacaagggcg tttacatcgg
     5041 catggaccgc tacgggctgg acctcttcct ccccaccgct caacgcaacg cgacggtgat
     5101 cgaactgctg aaccgcggct acgcggaccg catgtttctc tcccaggatt tcgacatccc
     5161 gatcgccaac ggcctcgact ggtacccgct cgaggtgctc gaacagcttc aggcggccgg
     5221 cgcagcgacc gactggagca tgaccttcat cttcgagcag gtgatcccgg cgctcaggga
     5281 agtcggaatg actggcgatc agctcgacac gatgctggtc gagaatccga agcggtggct
     5341 gggtcgtggc tgaggctgac cgcgggtcag tccacgacca tcgacatcga tccccggagg
     5401 gaaagagaat tgttcagcga caccccggtg gcgtttgcct cgaccgcaaa gaaaccgact
     5461 tcgccggcag agacatcgag accgatgttg tcgcacgcga aggtggaaat ctgaccgggc
     5521 ggcactactc ccgtaagggc aggtgaagcc gtgcacaagg tcgccggctg ctgaatcggc
     5581 tcggcgaacc cgtcttcgct ggtgtagagc cccccggtaa gtgaaatggt cgtgcccacc
     5641 aggttcaggg tcgacatcgt gtagagccaa ccgtttatcg aggagaccac gccgtccctg
     5701 ggaatgacct gcatgaccgt gagcgtgtcg ttcatcgaga acggttcctg catttgctgc
     5761 ggcgaagcca gggtgccgct gagcggcatc agggcataga agtcggggcc gccagagggc
     5821 acgcttgtca caggactcaa gggagcgttc gccgtgaaca ccatcgaacc ggttgcggtc
     5881 ccggtcgggc cggtcgggcc gacatcgccg gtcactccgt gagcgcccgg ggacccggtc
     5941 gctccggtta ctccaattgc gccgggcagg cctgccggcc cgcgagcccg gcgaacccgg
     6001 tcccctcggc ccggtggcac ctctcggcag ctggcccttc tgtccagatc aggcggccat
     6061 ggtgtcgaac ctgtcgtccg cgaacggaag cgcctcgacg ttttctgaag ggcccttctc
     6121 cttcctgccg aagccaagga acgatttgac tttcccgacc gagtcgttcg gaagcgagag
     6181 gcggatcacc ttcttccagg ccgaagccac ctggcggggc aggctgcctt ccgcgtaggg
     6241 caggtcgtag cggtcgaaga tctccctgac cttcggcgag atctcctggt agcggttgct
     6301 gggcagatcc ggaaacaggt gatgttcgat ctggaaggag aggtttccgg tcattacatg
     6361 catcgccgga gagcccttga tgttcgccgc gccaagcatc tggcggacgt accagtcggc
     6421 ccgggtttcg ccttcgatcg acgccttctc gaaggtggcc acaccctccg ggaaatggcc
     6481 acacatgatc accgagtggg tccagaggtt ccggaccagg ttcgcggtca ggttcgcggc
     6541 gaaggtggtc ggcgcagagg gccccgacaa ggccggatgg accaggtagt ccttggtcat
     6601 ctgggcccgg atcttcctga tcacctgttt cgccttggct tggaattccg gattgtcccg
     6661 gcggcccttc cagttcttct tgagctggag gtcgtaggcg gcgatgctgt actcgaagag
     6721 gaaggcgttg atcaggttcc aggccggctg gccgaggtag aacggcaccc agcgctggtc
     6781 ctcgtcgacc cgcatgatcc cgtaaccgag gtcgtggtcc ttgccgatca cgttcgtgta
     6841 ggtgtgatgg atctcgttgt gggagtgctt ccagagatcg gccggagacg cgttgtccca
     6901 ctcccaggag gtcgagtgga tcttcgggtc ccgcatccag tcccactggc catgcatcac
     6961 gttgtggccg atctccatgt tctcgaggat cttggccacc gaaaggccgg cggtgccgat
     7021 cagccaggcc ggcggaaaga gcgagaagag cagaaccccc ggctacccag ttcgagtttg
     7081 cgctgcgtgt cgatcatccc gcggatgtag gcggcgtcat cttcgccacg ggtgtcgagc
     7141 acctcctggc ggatggcgtc aagttcgcgg ccgatctgct cgatgtcctc ccgtgagaga
     7201 tggccgatcg ggttttcggg atccttctgg attgcggtca ttgtttcctc agagttcgat
     7261 ttcgcaggtg ccggcagccg ctgacacgca ggtctggatg cggatgtcgt ctccggccga
     7321 ggcggtggtg acttcgccgg tccggaggtc ccggaccgcg ccctcgcgca gccgggaaac
     7381 gcagccgaag cagatgccca tccggcagcc ggagggcatc agcaccccgg cctgctcg
//
LOCUS       DAOQKN010000127         7399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_4919_length_7399_cov_5.719907, whole genome shotgun sequence.
ACCESSION   DAOQKN010000127 DAOQKN010000000
VERSION     DAOQKN010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 7399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7399
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4919_length_7399_cov_5.719907"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1117)
                     /locus_tag="PLJ64_08805"
     CDS             complement(<1..1117)
                     /locus_tag="PLJ64_08805"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNC93622.1"
                     /translation="MSVVEIRNPEGTELLGEVEPTDPAEVGGVVERALAARPAWSALP
                     VAERAAMLDRAADLLEPEQETLGRLLAQESGKPIGQAEFEVGGGIHFLRGNAIEGRRL
                     VGRMLPTEGNFGTEWDLAYTRREPLGVVAAILPFNFPVELFVEKCAAALVGGNAVVVK
                     PPLEDPLVVERFRQALIEAGVPADVIGAVHGDAEVGAALAGAHGVDAISLTGSTAAGV
                     AVATATAARLPFLHLELGGNNGCLVLDDADLDLVAAEILRGRVLMNGQACSASKRVIV
                     DRSLHDDLATRMAAAVSAQKVGPAPDAATTIGPLIHERAAAKVAAQVANAKGQGAEVA
                     AGSGESDGAYFAPTLLAGVPYSADVAIDGGSPDAQDRQ"
     gene            complement(1202..2638)
                     /gene="pyk"
                     /locus_tag="PLJ64_08810"
     CDS             complement(1202..2638)
                     /gene="pyk"
                     /locus_tag="PLJ64_08810"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNC93623.1"
                     /translation="MNQIHSETRRTKIVATVGPASWDPSVLEQMIIAGVDVFRLNFSH
                     GDEATHVRVIKDVRLISERLDKEVGILGDLPGPKLRVGEYPQGVVDIIHGSTVTITPD
                     DVPGTETLIPVDWPELAQALSVEDTVYLADGSIRLRVVANDGRTVECEVEVGGPLSSH
                     KGMNLPGVEAGLDSVSEDDLRWVEFAVANQLDYIAVSFVRKAEDLDPVLNKLKELRSD
                     IPIIAKIEKPQAADAVEEIVDRATGGIMVARGDLGIEVPLSKVPVLQKSLIGAAGRAS
                     KTVITATQMLASMVSAKRPTRAEVTDVATAIYDGTDAIMLSEETAVGEFPVEAVRVMD
                     QIARGTETDLPYWDWVLNRVPQDDQDVSDSVTQSAVIAAYRLNLQAIVVPTASGRTAK
                     VVAAHRPQVPVLAVTHSVRTQRQLKLIFGVRPVVNDQTTEIRNLLYECADDAVRFGAA
                     ASGDLIAIVAGLSDMQLGTNLFEVHRVP"
     gene            complement(2918..5407)
                     /locus_tag="PLJ64_08815"
     CDS             complement(2918..5407)
                     /locus_tag="PLJ64_08815"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNC93624.1"
                     /translation="MLRKSAYLALLLLSFLGLAVPVANAAPKDYSAIARNIIPSGQKG
                     TPPLDTTQAVMYDALTPLFNNVTDSDIKEYFKSEKLGTSTDGPYTTETVPKAGVTIQR
                     DKYGVPHVTATTYDGGIWAAGWIAAEDRGLLLQQARYNARVAAIDAPGLSAIGLVAGL
                     QNFQPSQRTEDEIAKQTDALKAAGSEGKAVLKDIDTYLTGINDYLDANSPATPDWTRN
                     DVYAVNALKDQFLGEGGGDEARRSQFLGGLIKRLGAKKGWKVFNDLRQHTNNESPTSI
                     DGRFAYSPIPSNKSGSVIIDPKSFKPVDVTPNTPEELSDRSSSNPLEPRHQASNTLMI
                     TKGRSATGRPLMVGGPQIGYFAPGLTYEIDMNAPGLKWRGATSAPFPGYLLIGRGQDF
                     ATTLTSASGDVIDQFAETLCGGSDTKYLYKGKCRTMGTFDAGTLNGDPVSFRTTVHGP
                     VVGYATVKGKKVAISSKRSSYGKDVLDLMFNRRLSNGSVKGPQSFFEAASKTPQTFNS
                     FYIDNKNVALYTSGKLPIRDKRVDPGLLTKGTGQYEWKGFLSKKGHPHGMNPKSGMMV
                     NWNNSVARGFGAADDEWGRNGSVQRVGLLTRMLNRNKSKNGKLNMADVVSAMNAGATQ
                     DVRAIFTVPLLAKLLKGSKAPSTQARQMLNQMVDWNKKNGSRLDRDGDNLIDAPGAAS
                     LDAAWPKIADAFMKPVIGSQLDELSSLFSRFDQPPSGQYSGWYQYFDKDIRALLGQKV
                     KSPFKVKYCGHGKKAACQKAVWNAIAAAGTEAAAQYGSENPAQWHVNTAYVDDDHRGE
                     QIKFSPLTLVTMRYTNRPSGIQQVISFNGHR"
     gene            complement(5572..5661)
                     /locus_tag="PLJ64_08820"
     tRNA            complement(5572..5661)
                     /locus_tag="PLJ64_08820"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5625..5627),aa:Ser,seq:gct)
     gene            5730..6557
                     /locus_tag="PLJ64_08825"
     CDS             5730..6557
                     /locus_tag="PLJ64_08825"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC93625.1"
                     /translation="MALKEERFDGGSTGIAWRRLGEGPPLLLINGYAATKDDWDPTFL
                     AGLAEKSQVICPDNRGVGDSARSVQEITIASMATDLVSLLDDLKVGEVDVAGWSMGGF
                     IAQKLVTWIPDRIKSLTLLSTDSGGTRAHLRSPEAEAALTDKSGTPEEQADRLIDLLF
                     PAGFAAQVKATAGEIVAASRARLDPEVLAEQEAAMAGWYGPSVPPIDQVSELAQKGLP
                     VLIAHGLDDQVIPARNSRVLSGALEDAWLARFPGGGHAFMAQEPDRLSGLMSLFLAR"
     gene            6596..>7399
                     /locus_tag="PLJ64_08830"
     CDS             6596..>7399
                     /locus_tag="PLJ64_08830"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNC93626.1"
                     /translation="MDQPGTRTHGELEEIFGEVEAPFAFVDLDAMWANGESMLQRAGE
                     KPIRVATKSLRCRPLIEQILDRDDRFQGLMTFTLPETLWLAERGMENLLLAYPTTDLE
                     ALGELALRSVALQGASPVVMVDCVEHLDLIESILGTQASPVRLCLELDAALWAYGGRV
                     KAGTLRSPIHSPEDAVALAEEIGRREKLELVGLMAYEAQIAGLGDRIPGRPFRSRAIR
                     SMQKKSRAEIAERRAETVAKVREVAPLEFVNGGGTGSLESTSAEEAVTEV"
BASE COUNT         1175 a         2416 c         2495 g         1313 t
ORIGIN      
        1 gctggcggtc ctgcgcgtcg ggcgagccac cgtcgatcgc cacgtcggcc gagtacggca
       61 cgccggccag cagggtcggg gcgaagtagg cgccgtcgga ttcgccggag ccggccgcca
      121 cctccgcccc ctggcccttc gcgttcgcca cctgtgccgc taccttcgcc gccgcgcgct
      181 cgtggatcag cggaccgatc gtggtcgccg cgtccggtgc cgggcccacc ttctgggcgc
      241 tcaccgccgc agccatccgc gtggccagat cgtcgtgcaa cgagcggtcg acgatcaccc
      301 gcttgctggc cgagcaggcc tgtccgttca tcagcacccg gcccctcagg atctcggccg
      361 cgaccagatc gaggtcggcg tcatcgagca ccaggcagcc gttgttgccg cccagctcga
      421 ggtggaggaa gggcagccgg gccgcggtgg cggtggcgac ggcgacgccg gccgcggtcg
      481 atccggtcag cgagatcgcg tccaccccgt gggctccggc cagggccgcc ccgacctcgg
      541 cgtcgccatg gacggcgccg atcacgtcgg ccggcacacc ggcctcgatc agggcctggc
      601 ggaagcgctc gaccaccagc ggatcctcga gcggcggctt gaccaccacc gcgttgccgc
      661 cgaccagggc ggcggcgcac ttctcgacga agagctcgac cgggaagttg aagggcagga
      721 tcgccgccac caccccgagc ggttcgcgac gggtgtaggc gagatcccat tcggtgccga
      781 agttgccctc ggtgggcagc atgcgaccga ccaggcgacg gccttcgatc gcattgccgc
      841 gcaggaagtg gatgcctccg cccacctcga actcggcctg gccgatcggc ttgcccgact
      901 cctgcgcgag caaccggccc agcgtttcct gctccggctc gaggaggtcc gcggcccggt
      961 ccagcatcgc ggcccgttcg gccaccggca gggcggacca ggcgggccgg gcggcgaggg
     1021 cccgttcgac cactccgccg acctccgccg ggtcggtcgg ctcgacctcg cccagcagct
     1081 cggtcccttc cggattcctg atctcaacaa cgctcaaatt cccacctcga ggtacgcagg
     1141 tacccaacta gacgtcagta tcaaagttca gcctacagaa ccaggttcaa agttccgaat
     1201 ttcagggtac gcggtgcacc tcgaacaggt tggtacccag ctgcatgtcg gatagtccgg
     1261 ccacgatcgc gatcaggtca cccgacgccg ccgccccgaa ccggaccgcg tcgtcggcgc
     1321 attcgtagag caggttgcgg atctcggtgg tctggtcgtt gacgaccggc cgcacgccga
     1381 agatcagctt gagttgccgc tgggtgcgca cgctgtgggt caccgccagc accggaacct
     1441 gcgggcggtg ggccgccacg accttggcgg tgcgaccgga agcggtcggc accacgatcg
     1501 cctgcaggtt cagccggtag gcggcgatca ccgccgactg ggtgaccgag tcggagacat
     1561 cctgatcatc ctgcggaacc cggttgagga cccagtccca gtacggcagg tccgtttcgg
     1621 tgccgcgggc gatctggtcc atgaccctga ccgcctcgac cggaaactcg ccgaccgcgg
     1681 tctcttccga gagcatgatc gcgtcggtgc cgtcatagat cgcggtcgcg acgtcggtca
     1741 cctcggccct ggtcgggcgt ttggccgaaa ccatcgaggc cagcatctgg gtcgcggtga
     1801 tcaccgtctt ggaagcccgg ccggctgccc cgatcaggct cttctgcagc acgggaacct
     1861 tggaaagcgg cacctcgatg ccgaggtcgc cgcgggcaac catgatcccg ccggtggccc
     1921 ggtcaacgat ttcctcgacc gcgtcggcgg cctgcggctt ctcgatcttg gcgatgatcg
     1981 ggatgtccga gcggagctcc ttcagcttgt tcagcacggg gtcgagatcc tccgccttgc
     2041 ggacgaagga aaccgcgatg tagtcgagct ggttggcgac cgcgaactcg acccagcgca
     2101 ggtcgtcctc ggaaaccgag tcgagcccgg cttcgacgcc gggcaggttc atgcccttgt
     2161 gcgacgagag cggcccgccg acttctacct cgcactcaac cgtccgcccg tcgttggcaa
     2221 ccacgcgcag gcggatcgag ccgtcggcca gatagaccgt gtcctcgaca ctcagggcct
     2281 gggccagttc gggccagtcg accgggatca gggtttcggt tccaggcacg tcatcgggcg
     2341 tgatcgtcac ggtcgaaccg tggatgatgt ccaccactcc ctgcgggtat tcaccgaccc
     2401 ggagctttgg ccccggcagg tccccgagga tcccgacttc cttgtcgagc cgttccgaga
     2461 tcaggcgaac gtccttgatt acccgtacgt gagtcgcctc gtcaccgtga gaaaagttga
     2521 gccggaagac gtctaccccg gcaatgatca tctgctcgag cacggacgga tcccatgagg
     2581 cgggaccgac cgtggcgacg atcttcgtac gacgggtttc ggagtggatc tggttcaccc
     2641 ccggaatact ctcagtccgg cgggtccggc ctgtaaacgc ctgtaaacgg gggtcccgct
     2701 cgcgcggact gatcaaccta tccgcagttg aacggcggac ttcggttttc agttcggaat
     2761 ccggggtaaa ccgaaattac ggccttcatt ccaccggggc gatcccccta cgacccgatg
     2821 gataggcctg gcggtgagtg cctgcaccgc catggaaaga ggaaagcccg ccccagggat
     2881 ggggcgggct ttctcctcga tcgggctttg gccggaccta gcggtgaccg ttgaaggaga
     2941 tcacctgctg gatgccggac ggcctgttgg tgtagcgcat ggtgaccagg gtgagcgggc
     3001 tgaacttgat ctgctcgcct cgatggtcgt catccacgta ggccgtgttc acgtgccact
     3061 gggccgggtt ctctgagccg tactgggcgg ccgcttcggt gccggcggcg gcgatcgcat
     3121 tccagaccgc cttctggcag gcggccttct tgccgtggcc gcagtacttg accttgaacg
     3181 gactcttgac cttctggccg agcagcgccc ggatgtcctt gtcgaagtac tggtaccagc
     3241 cggagtactg gccactcggc ggctgatcga accgtgagaa cagcgagctc agctcgtcga
     3301 gctgcgaccc gattaccggc ttcatgaacg cgtcggcgat cttcggccag gcggcgtcca
     3361 gcgaggcggc gcccggggcg tcgatcaggt tgtcgccgtc acggtccaga cggctgccgt
     3421 tcttcttgtt ccaatcgacc atctggttga gcatctgccg ggcctgggta ctgggggcct
     3481 tcgagccctt cagcagcttg gccagcagcg gcacggtgaa gatggcgcgc acgtcctggg
     3541 tcgcaccggc gttcatcgcc gaaacgacgt cagccatgtt cagcttgccg ttcttgctct
     3601 tgttccggtt cagcatccgg gtcagcaggc caacgcgctg gaccgaaccg ttgcggcccc
     3661 actcgtcatc ggcggcgccg aagccacggg cgacgctgtt gttccagttg accatcattc
     3721 ccgacttcgg attcatgccg tgcgggtggc ccttcttgga gaggaagccc ttccactcgt
     3781 actggccggt gcccttggtc aagaggcccg ggtcaacccg cttgtcgcgg atcggcagct
     3841 tgccgctggt gtagagcgcg acgttcttgt tgtcgatgta gaacgagttg aaggtctgcg
     3901 gggtcttcga ggcagcctcg aagaatgact gcggaccctt gaccgaaccg ttcgacaggc
     3961 gacggttgaa catcaggtcg agcacgtcct tgccgtagct ggagcgcttc gaggagatcg
     4021 cgaccttctt gcccttgacc gtggcgtagc cgacgaccgg accgtggacg gttgtcctga
     4081 aggaaaccgg gtccccgttc agggtgccgg cgtcgaaagt gcccatggtg cggcacttgc
     4141 ccttgtacag gtacttggta tcgctgccgc cacagagcgt ctcggcgaac tggtcgatca
     4201 cgtcgccgct ggccgaggtc agcgtcgtcg cgaagtcctg gccgcggccg atcagcaggt
     4261 agccggggaa cggggccgag gtggcaccgc gccacttcag accgggcgcg ttcatgtcga
     4321 tctcataggt gagaccgggg gcgaagtagc cgatctgcgg tccgccgacc atcagaggac
     4381 ggccggtggc cgaacggccc ttggtgatca tcagcgtgtt cgaggcctgg tgacggggct
     4441 cgagcgggtt cgaactgctc cggtcgctga gctcctcggg agtgttcggg gtcacgtcga
     4501 ccggcttgaa gctcttcggg tcgatgatga cgctgccgga cttgttcgag gggatcggcg
     4561 agtaggcgaa acggccgtcg atcgaggtcg ggctctcgtt gttggtgtgc tggcgcaggt
     4621 cgttgaagac cttccagccc ttcttcgcac cgaggcgctt gatcagaccg ccgaggaact
     4681 gcgaacggcg ggcttcgtcg ccgccgccct caccgaggaa ctggtccttc agcgcgttga
     4741 ccgcgtagac gtcgttgcgg gtccagtccg gggtggccgg gctgttcgca tcgaggtaat
     4801 cgttgatgcc ggtcaggtag gtgtcgatgt ccttgaggac tgccttgcct tcggagccgg
     4861 cggccttgag ggcgtcggtc tgcttggcga tctcgtcttc ggtccgctgg ctgggctgga
     4921 agttctgcag cccggcaacc aggccgatcg cgctgaggcc cggcgcgtcg atggcggcga
     4981 cccgggcgtt gtaacgggcc tgctgaagca gcaggccgcg gtcttccgcg gcgatccagc
     5041 cggccgccca gatgccaccg tcataggtgg tggcggtcac gtgcggcacg ccgtacttgt
     5101 cgcgctgaat cgtgacgccg gccttcggca cggtctccgt ggtgtaggga ccatcggtcg
     5161 aggtgccaag cttctccgac ttgaagtact cctttatgtc actgtctgtg acgttgttga
     5221 agagcggggt cagcgcgtcg tacatcacgg cctgggtggt gtccaggggc ggagtgccct
     5281 tctggccgga ggggatgatg ttgcgcgcga tcgcggagta atccttggga gcggcgttcg
     5341 caacgggaac ggcgagaccg aggaaactca agagcagtaa cgccagatag gcgctctttc
     5401 gaagcatgaa gtttctcctt ggtaccggtc cggagggcgg cattcggcgt cgccactcag
     5461 accgggttgt catgagggtt gattggaggc tactccgatc agaacacccg gattcgccta
     5521 tgggtgcggt ggagggtcgg ccggggccgg ttcagcgacg ccggatctga gcggagaggg
     5581 agggattcga accctcgata cggctataaa ccgcatactc ccttagcagg ggagcgcctt
     5641 cagccactcg gccacctctc cgggccgggg aattgtatga gttgctgaaa gatgcagtcg
     5701 gccaacccct tctgacctag acttcgccca tggcgctgaa ggaggagcgg ttcgacggag
     5761 gctccacggg gatcgcctgg cggcggctgg gtgaaggacc cccgctcctg ctgatcaacg
     5821 gctatgcggc gaccaaggat gactgggacc ccacctttct ggccgggctc gccgaaaaat
     5881 cccaggtgat ctgccccgac aaccgcgggg tcggagactc ggcccgcagc gttcaggaga
     5941 tcacgatcgc ctcgatggcg accgacctgg tttcactcct cgatgacctg aaagtcgggg
     6001 aggttgacgt cgccggctgg tcgatgggcg gattcatcgc ccagaaactg gtcacctgga
     6061 tacccgaccg gatcaagtcc ctgaccctgc tctccaccga cagcggtggc acccgggccc
     6121 accttcgcag ccccgaggcc gaagccgccc tcaccgacaa gtccggcacc ccggaggaac
     6181 aggccgaccg gttgatcgac ctgctcttcc cggcagggtt cgcggcccag gtgaaagcca
     6241 cggcgggtga gatcgtcgcc gcctcgcggg cccggctcga cccggaagtc ctggccgaac
     6301 aggaagccgc gatggccggg tggtacgggc cttcggtgcc gccgatcgac caggtttccg
     6361 aactcgccca gaagggtctg cccgtcctga tcgcacacgg actcgatgac caggtgatcc
     6421 ccgcccgcaa cagccgcgtc ctttcgggcg ctctcgagga tgcctggctg gcccgcttcc
     6481 ccggcggtgg tcacgccttc atggcgcagg aacccgatcg cctgagtggc ctgatgtcac
     6541 tgtttctggc ccgataggcg gcgaaggctc cgaacccggc tctagccttt ggccaatgga
     6601 tcagccaggc acgcgaaccc atggggagct ggaagagatc ttcggcgagg tcgaggctcc
     6661 cttcgccttc gtcgatctcg atgcgatgtg ggcgaatggc gaatcgatgc tccagcgggc
     6721 cggcgagaag ccgatcaggg tggcgaccaa gtcgctccgc tgccggcccc tgatcgagca
     6781 gatcctcgac cgcgacgacc ggttccaggg gctgatgacc ttcaccctgc cggagaccct
     6841 ctggctggct gagcgcggga tggaaaacct gctgctggcc tacccgacca ccgacctgga
     6901 agcgctcggc gaactcgcgc tccgttcggt cgccctccag ggggcatctc cggtggtgat
     6961 ggtcgattgc gtcgagcacc tcgacctgat cgagtcgata ctcggcaccc aggcttcacc
     7021 ggtccgcctc tgtctcgaac tcgatgccgc gctctgggcc tacggcggca gggtcaaggc
     7081 cggcaccctg cgctccccga tccactcacc ggaggatgcg gttgcgctgg ccgaggagat
     7141 cgggcgccgc gaaaaactcg agctggtcgg gctgatggcc tacgaagcgc agatcgccgg
     7201 cctcggcgac cggatcccgg gccggccctt ccgttcccgg gcaatccgct cgatgcagaa
     7261 gaagtcgcgg gccgaaattg ccgaacggcg ggccgagacg gtggcgaagg tccgggaggt
     7321 tgcgccgctc gagttcgtaa acggcggcgg taccggctca ctcgagtcga cctcggccga
     7381 ggaggcggtg accgaggtg
//
LOCUS       DAOQKN010000128         7345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_4978_length_7345_cov_6.520988, whole genome shotgun sequence.
ACCESSION   DAOQKN010000128 DAOQKN010000000
VERSION     DAOQKN010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 7345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7345
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4978_length_7345_cov_6.520988"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /locus_tag="PLJ64_08835"
     CDS             complement(<1..1040)
                     /locus_tag="PLJ64_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNC93627.1"
                     /translation="MKRAIFEPEHQDFRETVGRFVTDEIMPNFEQWEEDGIVPRELFQ
                     KAGEKNMLAMSVSEEYGGLGLKDFRFNQIVGEEGYYQGATGAVLGVTLHNDVCLPYFE
                     EYCTDEQKERWLPGIVDGSLITAVAMTEPGIGSDLASMSTKAIRNDDHYVVDGSKTFI
                     TNGINADLIITAVKTDPKEKHRGMSIIIIERGMDGFDRGRNLEKVGMHSQDTAELFFN
                     EVKVPVENLLGNEGDGFKYLVSNLAQERMSIAVAAVATAQAALNWTLEYVKERYAFGS
                     PIGTFQNSRFQMAEIKTEVDLATVFIDRCVEALNEGTLTVEDAAEAKWWTTEMADRVI
                     DKCVQLHGGYGY"
     gene            1081..1851
                     /locus_tag="PLJ64_08840"
     CDS             1081..1851
                     /locus_tag="PLJ64_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC93628.1"
                     /translation="MKAESAIVFGGASGLGEATARALVEAGHKVVVADLNEEKGIELI
                     SEIGGTFVKADVTDEESVREAIAEAAGPGAGGASGGLRICVNCAGVGAAGRIVQKGEP
                     APLDGYKWAININLIGTINVLSQAAAAMVGNEPDGDGQRGVCINTASIAAYDGQIGQT
                     AYAASKGGVVGLTLPAARDLSRDGVRVMTIAPGLFDTPLLALLPEPARKALGETIPFP
                     PRLGVPEEFGALALHIVDNVMLNGEVIRLDGALRMAPK"
     gene            1929..2756
                     /locus_tag="PLJ64_08845"
     CDS             1929..2756
                     /locus_tag="PLJ64_08845"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNC93629.1"
                     /translation="MDLFQSIILGILQGLTEFLPISSSGHLLIFPALVGWDDPGAAFT
                     AVIQLGTEAAVLIYFRKDLWNIARDWTESIFKPEMRGELNPRMGWYLIIATIPISILG
                     LAFQDQIETAARNLWLVGSMLIIFGLVLGRADRVGTHQREVGELSFKDGVIIGLAQSL
                     ALIPGVSRSGATISAGLFLGLGREAAARFAFLLAIPAVVLSGFYELYKLLSGKESFDE
                     PMINIAVATLVAFVVGYAVIAWFLRYLTSHSMSVFVWYRVIVGTGVLILTAAGLISV"
     gene            complement(2859..3302)
                     /locus_tag="PLJ64_08850"
     CDS             complement(2859..3302)
                     /locus_tag="PLJ64_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93630.1"
                     /translation="MIPFRDNLDLRGPIWGTLALLFVYLVLALIGDIPHMNAWQVLVG
                     LVGLWLFAPYVERRAGTPLFLTGFVIVAGATGFLVGAVDEASGPYAISFFLPVLATAG
                     VHIALAPRSKILCLIPVPFAMTFVEIPTIAMTIIWVALEMLLTAA"
     gene            complement(3334..4323)
                     /gene="lipA"
                     /locus_tag="PLJ64_08855"
     CDS             complement(3334..4323)
                     /gene="lipA"
                     /locus_tag="PLJ64_08855"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNC93631.1"
                     /translation="MSTPPPDSATLEGSPAERIHVTRSRANPGGGTVQPDDAVPFRSR
                     KPSWLKVPPPGGPVYREIKQMIDGESLNTVCEEANCPNIGECWERGTATFMILGDVCT
                     RRCGFCNVQTGKPTHNDPLEPARVAMQVSRLGLSHAVITSVDRDDLPDYGASAFVGVI
                     RSIRAQAPDCKVEILTPDFRGQEMPLARVVHERPDVFNHNVETVPRLYPKARRGSKFL
                     RSARVLRLAREMGEGEVITKSGLMVGLGEEFDEMVETFGILREHGVQILTVGQYLRPT
                     VNHLPVVRYWHPEEFEALEKAAYEIGFESVAAGPLVRSSYHADESARKAAEAA"
     gene            complement(4330..5133)
                     /gene="lipB"
                     /locus_tag="PLJ64_08860"
     CDS             complement(4330..5133)
                     /gene="lipB"
                     /locus_tag="PLJ64_08860"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HNC93632.1"
                     /translation="MAALTVIELGRRDYAGMVEVQERLAADRLAGSIGDLLLLLEHPP
                     TYTLGRRSEPSDLLHGAGWYRERGIEVSRTPRGGKVTYHGPGQLIAYPIVDLRGVGEQ
                     PAGADRVDVARFVASLEGAMADSLWELGIPAGRIEGLTGLWVDDERRDPGDFTDADAA
                     SAAPGLARGSIRKIGSIGLKIRRGVTSHGLSLNVSCDLEPFDWINSCGIETCRATSIA
                     VELGGEAPSVESVGKAVVASLASRLGLEPTTGNPADFGLAEANPANSVA"
     gene            complement(5115..5546)
                     /locus_tag="PLJ64_08865"
     CDS             complement(5115..5546)
                     /locus_tag="PLJ64_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93633.1"
                     /translation="MSAGATPDATGDGPRCVTNSAGVAAGRRVASSAGVAGSVAVTHS
                     AGVAGSAATGFSAGVAGSLATLFSSGIAFSFAVLGSSVLVACAFCVGCFRCRRCAFCV
                     GCTNCVDCVGCVGCRGLIGARGVVGKSDEEQKGIRRWLPSR"
     gene            complement(5543..6262)
                     /locus_tag="PLJ64_08870"
     CDS             complement(5543..6262)
                     /locus_tag="PLJ64_08870"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HNC93634.1"
                     /translation="MSEEVQAPTSPAGNKGESEFVEYSRARQSMSRRIAESKATIPHI
                     YLQVEADVTALVRRRAALRAEGETATVNDLVIKATALALRNHSRVNSTYRDGGLELHS
                     RINIGVAVDTPDGSLTPVVTDADSKDLNEIATEVRDLSARARSGEITPPEQAGATFAI
                     SNLGTLGVTSSEPVVNPGQAAKLAVGAISSRPVVRGGEVVPGEVMTLDLSCDHRVLHG
                     GEAARFLNEVKANLENPGLLG"
     gene            complement(6318..7079)
                     /locus_tag="PLJ64_08875"
     CDS             complement(6318..7079)
                     /locus_tag="PLJ64_08875"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HNC93635.1"
                     /translation="MEGAASGASEETARKPDQAPVDSAKGTPEIVELNRTQQLIARRM
                     SESKATIPHFYLRAVVDMDRAVEVRAAFKEEAADNETVPSLNDFVVKAAAIALKRHPL
                     ANAAFRDGHFELYPKVNVGIAVATEGSLIVPVIKDADHLTLTEISDETRRLAGRVRSG
                     EVTPPELAGGTFTVSNLGMFGVSGFDAVINPGQAGILSVGEVTERPVVRDGKIASARL
                     MEITLACDHRILYGAEGAEFLASVKTNLEEPGLLA"
     gene            complement(7086..7223)
                     /locus_tag="PLJ64_08880"
                     /pseudo
     CDS             complement(7086..7223)
                     /locus_tag="PLJ64_08880"
                     /inference="COORDINATES: protein motif:HMM:NF014832.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
BASE COUNT         1195 a         2424 c         2476 g         1250 t
ORIGIN      
        1 atgtagccgt agccgccgtg gagctggacg cacttgtcga ttacccggtc ggccatctcg
       61 gtggtccacc acttggcctc ggccgcatcc tcgaccgtga gcgtgccttc gttcagcgcc
      121 tcgacgcagc ggtcgatgaa gacggtggcc aggtcgacct cggtcttgat ctccgccatc
      181 tggaagcgcg agttctggaa ggtgccgatc ggcgagccga aggcgtagcg ctccttgacg
      241 tattcgaggg tccagttgag cgcggcctgg gcggttgcaa ccgcggcgac cgcgattgac
      301 atccgctcct gggcgaggtt gctgaccagg tacttgaagc cgtcgccttc gttgccgagc
      361 aggttctcga ccggcacctt gacctcgttg aagaagagct cggccgtgtc ctggctgtgc
      421 atgccgacct tctccaggtt gcggccgcgg tcgaagccat ccatgccgcg ctcgatgatg
      481 atgatcgaca tcccgcggtg cttttccttg gggtcggtct tgaccgcggt gatgatcagg
      541 tcggcgttga tcccgttggt gatgaaggtc ttcgagccgt caaccacata gtgatcgtcg
      601 ttccggatcg ccttggtgga catgctcgca aggtcggagc cgatccccgg ctcggtcatc
      661 gcgaccgcgg tgatcaggct gccgtcgacg atgccgggca gccagcgttc cttctgctcg
      721 tcggtgcagt actcctcgaa gtagggaagg cagacatcgt tgtgcagggt gacgccgagc
      781 accgcgccgg tcgcgccctg gtaatagccc tcctcgccga cgatctggtt gaagcggaag
      841 tccttcagtc cgagcccgcc gtactcctcg gaaaccgaca tcgccagcat gttcttctcg
      901 cccgccttct ggaagagctc gcgagggacg atgccgtcct cctcccactg ctcgaagttc
      961 ggcatgatct cgtcggtgac gaagcggccg acagtctcac ggaagtcctg atgttcgggt
     1021 tcgaagatcg ctcgtttcat gcgcccaact ctatggcaac aacagcgagg agcaggacag
     1081 atgaaggctg aaagcgcgat cgtctttggt ggtgcctccg gactcggcga ggcgactgcg
     1141 agggccctgg tcgaagcagg tcacaaggtg gtggtcgccg acctgaacga ggagaagggg
     1201 atcgagctga tcagcgagat cggcggcacc ttcgtcaagg ccgacgtgac cgacgaggag
     1261 tcggtgcgcg aagcgatcgc ggaagccgcg ggccccgggg ccggcggagc cagcggtggc
     1321 ctcaggatct gcgtcaactg cgccggcgtc ggcgcggccg ggcggatcgt ccagaagggc
     1381 gagccggccc cgctcgacgg ttacaagtgg gcgatcaaca tcaacctgat cggcaccatc
     1441 aacgtgctga gccaggccgc cgcggcgatg gtcggcaacg agccggacgg tgacggccag
     1501 cgtggcgtct gcatcaacac cgcatcgatc gccgcctacg acggccagat cggccagacc
     1561 gcctacgccg cctcaaaggg cggagtggtc ggcctgaccc tgccggctgc ccgtgacctt
     1621 tcgcgggacg gagtgcgggt gatgacgatc gcgcccggcc tcttcgacac cccgctgctc
     1681 gcgcttttgc cggaaccggc ccgcaaggcc ctcggtgaga cgatcccctt cccgccccgc
     1741 ctcggggtgc cggaggagtt cggcgcgctg gccctccaca tcgtcgacaa cgtgatgctg
     1801 aacggcgaag taatccggct cgacggtgct cttcgcatgg ccccgaagta gggcggatct
     1861 gaacgaaagc tgaacggacg gagccggccc ggtccgggca ggcctccggc cgatagcctc
     1921 tccgagtcgt ggacctgttc cagagcatca ttctcggcat cctccagggg ttgaccgagt
     1981 tcctgccgat ctcctcgagc gggcacctgc tgatcttccc ggcgctggtc ggctgggatg
     2041 acccgggcgc cgccttcacc gcggtgatcc agctgggaac cgaagccgcg gtgctgatct
     2101 atttccgcaa ggacctctgg aacatcgcca gggactggac cgagtcgatc ttcaaacccg
     2161 agatgagggg ggagctcaac ccccgcatgg gctggtacct gatcatcgcc acgatcccga
     2221 tctcgatcct cggcctcgcc ttccaggacc agatcgagac cgccgcccga aacctctggc
     2281 tggtcggctc gatgctgatc atcttcggcc tcgtgctcgg ccgggccgac cgggttggca
     2341 cccatcagcg ggaggtcggc gaactctcct tcaaggacgg cgtgatcatc ggcctcgccc
     2401 agtcgctggc cctgatcccc ggcgtttccc gttccggcgc cacgatcagc gccggtctct
     2461 tcctcgggct cggtcgcgag gcggccgctc gtttcgcctt cctcctggcg atcccggccg
     2521 tcgtcctctc cggcttctac gagctataca aactgctctc gggaaaggaa agcttcgacg
     2581 agccgatgat caacatcgcg gtcgccaccc tggtcgcctt cgtggtcggc tacgcggtga
     2641 tcgcctggtt cctcaggtac ctgaccagcc actcgatgtc agtcttcgtc tggtaccggg
     2701 tcatcgtcgg caccggcgtc ctcatcctca ccgccgccgg tctgatctcg gtctgacccg
     2761 aaattgctag tcgaggccgc cgctggcctc cagacgcctg catcttgcct gggttcaagt
     2821 cattagggct atgtccgagt gaattgaacg gaggagggtc aggcggcggt gaggagcatt
     2881 tcgagggcga cccagatgat cgtcatggcg atcgtgggga tctcgacgaa ggtcatcgcg
     2941 aaggggaccg ggatcaggca gaggatcttc gatcggggag ccagggcgat gtggacgccg
     3001 gcggtggcga ggaccggaag gaagaagctg atcgcgtagg ggcccgaggc ctcgtcgacc
     3061 gcgccgacca ggaaaccggt ggctccggcg acgatcacga aaccggtcag gaagaggggg
     3121 gtgccggccc ttcgctcgac gtatggggcg aacagccaga ggccgaccag gccgaccagg
     3181 acctgccagg cgttcatgtg ggggatgtcg ccgatcaggg ccaggaccag atagacgaac
     3241 aaaagggcga gcgtgcccca gatcggacca cgcaggtcca ggttgtcgcg aaacggaatc
     3301 aaggaatcag cgagggtacc gggagaaagc ggttcaagcc gcttcggcgg cttttcgtgc
     3361 gctctcgtct gcgtggtaac tgctgcgaac cagcggaccg gccgcgaccg actcgaagcc
     3421 gatctcgtag gcggccttct cgagggcctc gaattcctcg ggatgccagt agcggaccac
     3481 cggcaggtga tttacggtcg gccgaaggta ctggccgacc gtgaggatct ggaccccgtg
     3541 ctcccgcagg atgccgaagg tctcgaccat ctcgtcgaac tcctcgccga ggccgaccat
     3601 caggccggac ttggtgatca cttccccttc acccatctcg cgggccaggc gaaggaccct
     3661 ggccgagcgc aggaacttcg agccgcgccg ggccttcggg taaagccgcg gcaccgtctc
     3721 gacgttgtgg ttgaagacat cgggacgctc atggaccacc cgggccaggg gcatctcctg
     3781 gccacggaag tccggggtca ggatctcgac cttgcagtcc ggggcctggg cacggatcga
     3841 acggatcacg ccgacgaagg cggaagcacc gtagtccggc aggtcatccc ggtcaaccga
     3901 ggtgatcacc gcatgcgaaa ggccgaggcg actcacctgc atcgcgaccc gggccggttc
     3961 cagcggatcg ttgtgggttg gcttgccggt ctggacgttg cagaagccgc agcggcgggt
     4021 gcagacgtcg ccgaggatca tgaaggtggc ggtgccgcgt tcccagcact cgccgatgtt
     4081 cgggcagttc gcttcctcgc agaccgtgtt caggctctcg ccatcgatca tctgcttgat
     4141 ctcgcggtag accgggccgc cgggcggcgg tactttcagc caggaaggct tgcgggaacg
     4201 gaacggcacc gcgtcatcgg gctggacggt gccgccgccg ggattcgccc gggagcgggt
     4261 cacgtggatc cgctcggcgg ggctgccttc gagggtcgcg ctgtcggggg gcggggtgct
     4321 caccgccacc taggctacgg aattggcggg attcgcttcg gccaggccga agtcggcagg
     4381 attgccggtg gttggctcca gtccgaggcg cgaggccaga ctggccacca cggcttttcc
     4441 gaccgactcg accgaaggtg cctcgccacc gagctcgacc gcgatcgagg tcgcccggca
     4501 ggtctcgatg ccgcaggagt tgatccagtc aaacggctcg agatcgcagg aaacgttcaa
     4561 cgaaaggcca tggctggtca cgccccgccg gatcttcagc ccgatcgagc cgatcttgcg
     4621 gatgctgccc cgggccagcc ccggggcggc cgacgcggcg tcggcgtcgg tgaagtcccc
     4681 cgggtcccgg cgttcatcgt caacccagag cccggtcagg ccctcaatcc ggcccgccgg
     4741 gattcccagc tcccagaggc tgtcggccat cgcgccttcg agtgaggcga cgaagcgggc
     4801 cacgtcgacc cggtcggccc cggcgggctg ctccccaacc ccccgcaggt cgacgatcgg
     4861 ataggcgatc agctggcccg ggccgtggta ggtgaccttg ccgccccgcg gtgtccggct
     4921 gacttcgatg ccgcgctcgc ggtaccaccc ggctccgtga agcaggtccg acggttcgga
     4981 gcggcggccc agggtgtagg tgggcggatg ttcgagcagc agaaggagat caccgatcga
     5041 gccggcgagc cggtcggcgg ccagccgttc ctggacttcg accattccgg cgtaatcgcg
     5101 ccggccgagt tcgatcaccg tgagggcagc caccgccgga tccctttctg ctcctcatcc
     5161 gacttgccga cgacgccacg ggctccgatg agtccccggc aaccgacaca gccaacgcag
     5221 tcgacgcagt tcgtgcagcc gacgcagaaa gcgcagcggc ggcaacgaaa gcacccgacg
     5281 cagaaggcac aggccaccag aacggaggag ccgagaacgg cgaagctgaa tgcaatcccg
     5341 gagctgaaca gggtcgccag gctgcccgcg acgccggcgc tgaatccggt cgcggcactg
     5401 ccggccactc cggccgagtg ggtcaccgcg acgctgcctg cgactccggc cgagctcgct
     5461 acccggcgtc cggccgcgac gccggcactg ttggtcacgc agcgggggcc atcgccggtc
     5521 gcatccggtg tggccccggc gctcatccca gcagccccgg gttctccagg ttggccttga
     5581 cctcgttcag gaagcgggcc gcctcgcccc cgtgaagcac gcggtgatcg caggacagat
     5641 cgagggtcat tacctcgccg ggcacgacct cgccgccgcg gacgaccgga cgcgaactga
     5701 tcgccccgac cgcgagcttc gccgcctggc cgggattgac caccggctcg gagctggtca
     5761 cgccaagcgt gccgaggttg gagatcgcga aagtggcgcc ggcctgctcg gggggagtga
     5821 tctcgccgga gcgggcccgg gcggaaaggt cccgcacctc ggtcgcgatt tcgttcagat
     5881 ccttgctgtc ggcgtcggtg acgaccgggg tcagcgagcc gtccggggtg tcgacagcga
     5941 ccccgatgtt gattcgcgaa tgaagttcga gcccaccgtc acggtaggtg ctgttgaccc
     6001 ggctgtgatt gcggagggcc agcgccgtcg ccttgatcac caggtcgttc acggtcgccg
     6061 tctcgccttc ggcccggagg gccgcccggc ggcggaccag tgcggtgacg tcggcctcga
     6121 cctgcaggta gatgtggggg atggtcgcct tcgactcggc gatccgccgc gacatcgact
     6181 gtcttgcccg tgagtactcg acgaactcgc tttcgccctt gttgccggcc ggactggtgg
     6241 gtgcctgtac ctcttcgctc atctggagtg acgatatggg catccggact ccggtgtcgg
     6301 ccccgcggcg cccgtcttca ggcgagcagg ccgggttcct cgaggttcgt cttgaccgag
     6361 gcaaggaatt cggctccctc ggccccgtag aggatgcggt gatcacaggc gagcgtgatc
     6421 tccatcaggc gggcggaggc gatctttccg tcccgcacga ccggacgctc ggtgacctct
     6481 cccaccgaga ggatgcccgc ctgcccgggg ttgatcaccg cgtcgaatcc gctcaccccg
     6541 aacatgccga ggttcgagac ggtgaaggtg ccgccggcca gctcgggagg cgtgacctcg
     6601 ccggagcgga cccgcccggc aaggcgccgc gtttcatcgc tgatctcggt cagggtcagg
     6661 tggtcggcgt cctttatcac cggcacgatc agcgaaccct cggtcgcaac tgcgatcccg
     6721 acgttgacct tggggtagag ctcgaagtgg ccgtcccgga aggcggcgtt ggccagcgga
     6781 tgccgcttca gggcgatggc cgcagccttg accacgaagt cattcagcga ggggacggtc
     6841 tcgttgtcgg cggcttcttc cttgaaggcg gccctgacct cgaccgcgcg gtccatgtcg
     6901 accacggcgc ggagatagaa gtgagggatg gtcgccttcg actccgacat gcgccgcgcg
     6961 atcaactgct gggtccggtt cagctcgacg atctcgggcg tccccttggc cgagtcaacc
     7021 ggggcctggt ccggtttcct cgcggtctcc tccgacgccc cgctcgccgc gccttccaca
     7081 tcaaccctga tgatccgacc cccgggaccc gaccccgaaa gaccggtcag atctactccc
     7141 aaatcctccg cgatacgtct tgccagaggc gaagctttga tccgttcacc cgagttggac
     7201 tcaccatcgg aaacagaagg cattgaaggg gaatcttctg aggtctcgcg caggtctggc
     7261 gaaccctctt cggagctgga agcggtggcc gttgggggtc ccggcggaac tcccggcgaa
     7321 gtccggccga cgggatcccc aacgg
//
LOCUS       DAOQKN010000129         7330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_4993_length_7330_cov_6.466942, whole genome shotgun sequence.
ACCESSION   DAOQKN010000129 DAOQKN010000000
VERSION     DAOQKN010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 7330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7330
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4993_length_7330_cov_6.466942"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="PLJ64_08885"
     CDS             <1..982
                     /locus_tag="PLJ64_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93636.1"
                     /translation="LKRLLVGGVQRLSGQGGDNLDGGLGENDCSGGSHTNCNENSPGN
                     QRGQTQVDIMEGGIPIVLGSNQADDISVGFNRGAGRFEVAVAGGALPSGLCEKANPAG
                     SQINCPADPNDLNGLLVYGDNGADQIKLENSIPATLTTTVNGGTGNNTIDGGPSKDFI
                     STAVGSAGSTINGNDGLDVLYAQDRVTVNGGDDTDIFRVVDPCLGAELRGNAGTDSVV
                     FAGAPNGVKASLAGGYAEWRNGGCGSDTDIARDIEKLEGTDYNDWLIVGKRNSSQDGR
                     SVLFGRGGIDILDAKNGSVDQITTGAGGRNNTVIADKNDRITWGYGLGDY"
     gene            1121..3121
                     /locus_tag="PLJ64_08890"
     CDS             1121..3121
                     /locus_tag="PLJ64_08890"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HNC93637.1"
                     /translation="MFEGKSAGRLVRILVLALALLVAAAGQLFLTARAEARPSSCFGK
                     TVNRVVKSSNTTVHLAYRDVAWVEGTGVTVIGKPYSRICAGGGRQVIHAGKGTSFTDA
                     GPGNDRIVLHPKSNDNQVHGGAGDDEIDGSSGNDFIYGSPRRLPSGVSDSDDLVGKGG
                     NDHIYDYGGTGNQLRGLTGSDRLYSLGRAVSQVHGGNGTDFIFSNGGKSRSGVIEKVF
                     GEQGNDRIRADRTPNNGPAYLDGGEGDDWMTGSKRDDIFIYNAGIKKLRGLGGDDLFV
                     TTGRGLGRINGGGGTDTISFAALTSSNAYVPGMGPISGARVDLRTGRSIGYTTYRLSA
                     IEDVIGSAFDDWIRGKSGTAHIDGGLGHNICSGGRELQNCNGNSPGGGNGSRAIVDIN
                     ESGFPIVMGSRGADQVKVGFNGASSRFEVQVAGGAAVSGLCRRANSSGSRINCPANRN
                     NLNGLTISTGDGGDSVKLLGSIPATMTTKIDGGNGRNVIQGGRSKDLIETSTSSAGSV
                     LKGDGGLDIIYANDRVRVMGGGDTDISRVVNPCRGATLDGGPGTDSVVFAGPHARRGV
                     KADLGRGYAQWRSGGCAARTDIGPDIEKLEGTDGNDYLIVGRRHRAQQGRSVIFGRGG
                     VDLFDARNGRRDTVTTGANGHRNKVRADRIDKVIWGYGLAGY"
     gene            3189..5414
                     /locus_tag="PLJ64_08895"
     CDS             3189..5414
                     /locus_tag="PLJ64_08895"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HNC93638.1"
                     /translation="MFPAFCFSRQSRLKAIGAGLMLAAALFLMPLPGHAEARPSTCFG
                     KKINRVVGGSGQTVRLRFRDVAWVAGDRVTVIGKPFSHICAGDGPQTIYTGKGITFTD
                     AGAGSDRIFAAKGNATVNGGPGNDFIRTEKGNILRVSGGSGDDDIRTGKGNSVEIDGA
                     AGDDRIALNSKTTRGVIHGGSGNDYLRGSRSHDRIFAGPTRNPGRLPDRDTVLGVGGN
                     DRIYDYSGTGNRLYGLTGSDRIYSLGNAVSEIHGGNGTDFLFANGGVTGSGVREKLFG
                     EQGNDRLRADQPNNNGPAYLDGGEGDDWVFGTPKNDTIITHSGIKKLNGNAGDDLFVT
                     TARGLARVNGGSGNDTISYAAHTPPGGRRITGVIVDLRAGTSLGTTRYRLTSLENVIG
                     SAFNDEITADPGVTNNIEGGLGNDLIVGNDGDGDSVDGGLGVNDCSGFAKSTRCNRES
                     PGNFGNRRTLVDISDGGIPIVMGGTQGDRIFVGYDGSNQWFRIIVADGGVPSGNCRGV
                     NQSPDSAIAQKIRCPANRNNLDGMLVYGGGGADRINVENSVPPDMSTTLNGGDGKNVI
                     HGGRSKDFIQTSFDSTGSEIFGGGNNDLMYANDRVTIHGGGGTDHAQILRPCSGSLYS
                     GGPGNDSAVFAGSPRGVKVDLGRGYVEWYHGHCGARTTLRPDIERLEGSPHKDWMILG
                     HRHRQQQGRSSLLGREGVNILNSRNGRRDTVTTGPAAHRNKVIRDRRDKVVWGWGLAA
                     F"
     gene            5447..6073
                     /locus_tag="PLJ64_08900"
     CDS             5447..6073
                     /locus_tag="PLJ64_08900"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNC93639.1"
                     /translation="MTSDRARRELDSRELPAGQFARLLKAVVVPRPIAWVSTTSAEGV
                     DNLAPHSFFTFASEVPPVLQFTSIGHKDSLRNATGTGEFVINLADLGNLDAVNASGTA
                     YPPDEDEFAALGIEREPSVAVRPPRVAGSPVAIECRTEGTVEFDESVVVFGRVVHLAI
                     SEAVLDGDHPDIGKLAPMARLGRNEWSEIGKVHRIDRIPLDRARRNGS"
     gene            complement(6136..>7330)
                     /locus_tag="PLJ64_08905"
     CDS             complement(6136..>7330)
                     /locus_tag="PLJ64_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HNC93640.1"
                     /translation="RTMQMHRGGKVSVENKYPLKTRDDLSMAYTPGVARVCTAINQDR
                     QLAFEYTIKKNTVAVVSDGTAVLGLGDIGPEAAMPVMEGKAMLFKEFAQVDAFPICLD
                     TTDTDEIVRAVEMIAPTFGGINLEDISAPRCFEIEDRLKESIDIPVFHDDQHGTAVVT
                     MAALFNALKIIDKPIEELRVLMVGLGAAGVAVTKMMLAAGLTHVVGCDRKGALSVTRE
                     DYQAGELSGIKKWFADNSNPDHLLGTPDEVIEGMDVFVGLSGPGIIQPESLGKMNRDP
                     IVFAMSNPDPEVAPEEAAKYVRIMATGRSDYPNQINNVLCFPGIFRGALDAGAPQITE
                     EMKMAAARGIADVVGDDLSEDYIIPSVFDRDVAPRVAEAVIQEAKRAGDARFNVETGA
                     FTALG"
BASE COUNT         1364 a         2412 c         2428 g         1126 t
ORIGIN      
        1 cctcaagcgg cttctggtcg gcggcgtgca gcgcctctcc ggccagggcg gcgacaacct
       61 cgacggcggg ctcggagaaa acgattgctc cggcggcagc cacaccaact gcaacgagaa
      121 ctcccccggc aaccagaggg ggcagacaca ggtcgacatc atggaaggcg gaatcccgat
      181 cgtcctcggc agcaaccagg ccgatgacat ttcggtcggc ttcaatcgcg gcgccggacg
      241 gttcgaagtc gcggtcgccg gcggggccct tccatcgggg ctctgcgaaa aggcgaaccc
      301 ggccggcagc cagatcaatt gcccggccga cccgaacgac ctcaacgggc tgctcgtcta
      361 cggtgacaac ggcgccgacc agatcaaact cgagaactcg attccggcga ccctgaccac
      421 gacggtcaac ggaggcaccg gaaacaacac gatcgacggc ggtcccagca aggacttcat
      481 ttcgaccgcg gtcggcagcg ccggcagcac gatcaacggc aacgacggcc tcgacgtcct
      541 ttacgcacag gaccgggtca ccgtcaacgg cggcgatgac acagacatct tccgggtcgt
      601 cgatccctgc ctcggagcgg agctgcgtgg caatgccggc accgacagcg tggtgttcgc
      661 gggtgccccg aacggcgtca aggcaagcct ggcaggcggt tacgcggagt ggcgaaacgg
      721 cggctgtggc agtgacacgg acatcgccag ggacatcgag aagctcgaag gaaccgacta
      781 caacgactgg ctgatcgtcg gcaagcggaa ctcctcccag gacgggcggt ccgtgctctt
      841 cggtcgcggg ggcatcgaca ttctggacgc gaagaacggc agcgtggacc agatcaccac
      901 cggcgccggc ggccgtaaca acacggtgat cgccgacaag aacgaccgga tcacctgggg
      961 ctacggactg ggcgattact gaggccccgc cccggatcgg gccggttcgc aggcgggatt
     1021 gtttacttcc gttcgcctgc gggaacaaca gtgacttcgc aactgttaca ggttgtcacg
     1081 aggagctccc tggacatgag tacccacgac gggagatcaa atgttcgaag gtaaatctgc
     1141 aggtcgtctg gtcaggatcc tggttctggc cctggccctt ctggtcgcgg cggccggtca
     1201 acttttcctg accgccaggg ccgaagcccg gccctccagc tgcttcggaa agacggtcaa
     1261 ccgcgtcgtc aagtccagca acacgacggt tcacctggcc taccgggatg tcgcctgggt
     1321 cgaggggacc ggcgtcaccg tgatcggcaa gccctacagc cggatctgcg ctggcggtgg
     1381 ccgtcaggtc atccatgccg gcaagggcac ctccttcacc gacgccggac cgggcaacga
     1441 caggatcgtc ctgcacccga agagcaacga caaccaggtt cacggcggcg ccggtgacga
     1501 cgagatcgac ggctcgagcg gaaacgactt catctacggg tctccccgtc gcctgccctc
     1561 cggggtcagc gacagcgacg acctggtcgg caagggcggc aacgatcaca tttacgacta
     1621 cgggggaacc ggcaaccagc ttcgcggcct gaccggctcc gaccgcctgt acagcctcgg
     1681 cagggcggtc tcccaggtgc acggcggcaa cggcaccgac ttcatcttct ccaacggcgg
     1741 caagagccga agcggagtga tcgagaaagt gttcggcgag cagggcaacg accgcatccg
     1801 ggccgaccgg acaccgaaca acggccccgc ctatctcgac ggaggcgagg gtgacgactg
     1861 gatgaccggc agcaaacgcg acgacatctt catttacaac gccggaatca agaagctgag
     1921 ggggctcggc ggagacgatc tcttcgtaac caccggccgc gggctgggca ggataaatgg
     1981 cggcggcgga acggacacga tttccttcgc tgcgcttact tccagcaacg cctacgtgcc
     2041 ggggatgggc ccgatcagcg gggcccgggt tgacctccgg accgggcgct cgatcggcta
     2101 cacgacctac cgcctgtccg cgatcgaaga tgtgatcggg tccgcattcg acgactggat
     2161 ccgcggcaag tccggcaccg cccacatcga cggcgggctg ggtcacaaca tctgttccgg
     2221 cgggcgcgag ctgcagaact gcaacgggaa ttcacccggc ggcggcaacg gctcgagggc
     2281 gatcgttgac atcaacgaga gcggtttccc gatcgtgatg ggcagtcgcg gggccgatca
     2341 ggtcaaggtc ggcttcaacg gcgcatccag ccggttcgag gtgcaggtcg ccggcggcgc
     2401 ggccgtctcc ggtctctgcc gcagggcaaa ctcttccggc agccggatca attgcccggc
     2461 caaccgcaac aacctgaacg ggctcacgat ctcgaccggt gacgggggag actcggtcaa
     2521 gctgctcggc tcgatcccgg ccacgatgac gaccaagatc gacggcggca acggtcgcaa
     2581 cgtgatccag ggaggtcgaa gcaaggacct gatcgaaacc tcgaccagca gcgccggcag
     2641 cgtgctcaag ggcgacggcg gcctcgacat catctacgcc aacgaccggg tccgggtgat
     2701 gggcggtggc gacaccgaca tctcccgcgt ggtcaacccc tgccgtggcg ccacgcttga
     2761 cggcggcccc ggcaccgaca gcgtcgtctt cgccggtcct cacgcccggc gcggcgtaaa
     2821 ggccgacctc ggccggggct acgcacagtg gcgaagcggc ggctgtgcgg cccggaccga
     2881 catcgggccc gacatcgaga agctcgaggg gacggacggc aacgactacc tgatcgtcgg
     2941 ccggcggcat cgcgcccagc agggccggtc ggtgatattc ggccgcggcg gagtggacct
     3001 gttcgacgca cgaaacgggc gcagggacac ggtcacgacc ggcgccaacg gtcaccgcaa
     3061 caaggtcagg gcggaccgga tagacaaggt gatctggggt tacggcctgg ccggctactg
     3121 agcaaaccgg gaatctcccg acgccgaaac tccggtcggt tcggggtgct atctaaagta
     3181 gatagacctt gtttccagcg ttttgcttct cccgacaatc ccgcctgaaa gcgatcggcg
     3241 ccggcctgat gctggccgct gccctgtttc tcatgccctt gcccggccac gccgaagccc
     3301 ggccttcgac ctgcttcggc aagaagatca accgggtcgt cggcggcagc ggccagacgg
     3361 tccgactcag gtttcgggac gtcgcctggg tggccgggga ccgggtgacc gtgatcggca
     3421 agccgttcag ccacatctgc gccggggacg gaccgcagac catctacacc ggcaagggca
     3481 tcaccttcac cgatgccggc gccggcagcg accggatctt tgcggcgaag gggaacgcga
     3541 ccgtaaacgg cggccccggc aacgacttca tccgtaccga gaagggcaac atcctccgcg
     3601 tttcaggcgg ttccggggac gatgacatcc gcacgggcaa aggcaacagc gtcgaaattg
     3661 acggggcggc cggcgatgac cgaatcgccc tcaactcgaa gaccaccagg ggggttatcc
     3721 acggtggctc cggcaacgac tacctgcgcg gatcaaggag ccacgaccgg attttcgccg
     3781 ggccgacccg caaccccggc cggctgccgg accgggacac ggtgctcggc gtgggcggca
     3841 atgaccgcat ctacgattac tccggcaccg gcaaccggct ctacggcctg accggctccg
     3901 accgcattta cagcctcggc aacgccgtct ccgagatcca cggcggcaat ggcaccgact
     3961 tccttttcgc gaatggcggc gtcactggca gcggcgtgag ggagaagctc ttcggcgagc
     4021 aaggcaacga ccggcttcgc gccgaccagc cgaacaacaa cggccccgcc tatctcgacg
     4081 gtggggaagg cgacgactgg gtgttcggca cgcccaaaaa cgacacgatc atcacccact
     4141 cgggcatcaa gaaactgaac gggaacgccg gcgacgacct cttcgtgacc accgcgcgcg
     4201 ggctggccag ggtgaacggc gggtctggca acgacacgat ttcctacgcc gcacacaccc
     4261 cgccgggcgg tcgccgcatc acgggagtga tcgtcgattt gcgggccggc acctcgctcg
     4321 ggacgacccg ctaccggctc acctcgctcg agaacgtgat cggttctgct ttcaacgacg
     4381 agatcaccgc cgatcccggc gtgaccaaca acatcgaagg cggtctcggg aacgatttga
     4441 tcgtcggcaa cgacggtgac ggggacagcg tcgacggcgg cctcggtgtg aacgactgtt
     4501 ccggcttcgc caagtcgact cggtgcaacc gcgaatcacc gggcaacttc gggaaccgga
     4561 ggaccctggt cgacatcagc gacggcggca tcccgatcgt catgggtgga acccaggggg
     4621 accggatatt cgtcggctac gacggctcga accagtggtt ccggatcatc gtcgccgacg
     4681 gcggagttcc ctccggcaac tgccggggcg tgaaccagtc accggactcg gcgatcgcgc
     4741 agaagatccg ctgccccgcc aatcgcaaca atctcgatgg gatgctcgtt tacggcggtg
     4801 gcggcgccga ccggatcaac gtcgagaact cggtcccgcc ggacatgtcc acgaccctga
     4861 acggaggcga cgggaagaac gtcatccacg gcggtcgcag caaggacttt atccagacca
     4921 gtttcgacag caccggcagc gagatcttcg gtggtggcaa caacgacctg atgtacgcga
     4981 acgaccgggt caccattcac gggggcggcg ggaccgatca cgcccagatt ctcaggccat
     5041 gttcagggtc cctctacagc ggcgggccgg gcaatgacag cgcggtcttt gccggctccc
     5101 cccgcggggt caaggttgac ctggggaggg gttacgtcga gtggtaccac ggtcactgcg
     5161 gcgcccggac cacgctcagg cccgacatcg aacggctgga gggcagcccg cacaaggact
     5221 ggatgatcct cggccaccgc cacagacagc agcaggggcg gtcctcgctg ctgggccggg
     5281 aaggcgtcaa catcctcaat tccaggaatg gtcgccgcga cacggtgacc accggccccg
     5341 ccgctcaccg caacaaggtg atccgcgacc gccgggacaa ggtcgtctgg ggctggggcc
     5401 tggccgcttt ctagccgttc cggctgcagt ccggcagaat ccgggcatga cctccgaccg
     5461 ggccagaagg gaactcgatt ccagggaatt gccggcaggg cagttcgccc ggctcctgaa
     5521 ggcggttgtg gtgccgcgcc cgatcgcctg ggtctcgacc acttccgccg agggcgttga
     5581 caacctggcc ccgcattcgt tcttcacttt cgcctcggag gttcctccgg ttctccagtt
     5641 cacctcgatc ggccacaagg actcacttcg caacgccacc gggacgggtg agttcgtgat
     5701 caacctggcc gacctcggca acctcgacgc ggtcaacgca tccgggaccg cctacccgcc
     5761 ggatgaggac gagttcgcgg cgctcgggat cgaacgggag ccctcggtcg cggtcaggcc
     5821 gccccgggtg gccggttcac cggtggcgat cgaatgccgg accgagggca ccgtcgagtt
     5881 cgacgaatcg gtggtcgtct tcggccgggt ggttcatctg gcaatcagcg aagcggtgct
     5941 ggacggagat catcccgaca tcgggaagct cgccccgatg gcccgtctcg gccgcaacga
     6001 gtggtcggag atcggcaagg tccaccggat cgaccggatt cccctggatc gggcgcgccg
     6061 aaacgggagc tagaccatcg aatgcccact ttgtgccggt atgtggcgca aagcgggttc
     6121 tcgccgcagg gagactcagc ccagggcggt gaaggcgccc gtctcgacgt tgaagcgcgc
     6181 gtcgccggcc cgcttggctt cctggatcac cgcttcggct acccggggtg cgacgtcacg
     6241 gtcgaagacc gaggggatga tgtagtcctc ggagaggtcg tccccgacca cgtcggcgat
     6301 gccccgggcg gcggccatct tcatctcctc ggtgatctgt ggcgcgccgg cgtcgagtgc
     6361 gccgcggaag attcccggga aacagagcac gttgttgatc tggttcgggt agtcggagcg
     6421 gccggtggcc atgatccgca cgtacttggc ggcctcctcg ggagcaacct ccgggtccgg
     6481 attcgacatg gcgaagacga tcggatcccg attcatcttg cccagcgact cgggctggat
     6541 gatgcccggg cctgacaggc cgacgaacac gtccatgcct tcgatcacct cgtccggagt
     6601 gccgagcaga tggtccgggt tcgagttgtc ggcgaaccac ttcttgatcc cgctcagctc
     6661 gccggcctgg taatcctcgc gagtgaccga aagggcgccc ttgcggtcgc agccgaccac
     6721 gtgggtgagg ccggcggcaa gcatcatctt ggtcaccgcc acgccggcgg cgccgaggcc
     6781 gaccatcagc acccgcagct cctcgatcgg cttgtcgatg atcttcagcg cgttgaacag
     6841 cgcggccatg gtcaccaccg cggtgccgtg ctggtcatcg tggaaaaccg ggatgtcgat
     6901 cgattccttg agccggtcct cgatctcgaa gcagcgcggg gcggagatgt cctcgaggtt
     6961 gattcccccg aaggtggggg cgatcatctc gaccgcacgg acgatctcgt cggtgtcggt
     7021 cgtgtcgagg cagatcggga aagcgtcaac ctgggcgaac tccttgaaga gcatcgcctt
     7081 gccctccatc acgggcattg cggcctcggg gccgatgtcg ccgaggccga gcaccgcggt
     7141 gccgtcggag acgaccgcca cggtgttctt cttgatcgtg tactcgaagg cgagctggcg
     7201 gtcctggttg atcgccgtgc agacccgggc gacacccggg gtgtaggcca tcgaaaggtc
     7261 gtcgcgtgtc ttcaaggggt acttgttctc gaccgaaacc ttgccgcccc ggtgcatctg
     7321 catggtgcgg
//
LOCUS       DAOQKN010000130         7259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_5060_length_7259_cov_5.040672, whole genome shotgun sequence.
ACCESSION   DAOQKN010000130 DAOQKN010000000
VERSION     DAOQKN010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 7259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7259
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5060_length_7259_cov_5.040672"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="PLJ64_08910"
     CDS             complement(<1..496)
                     /locus_tag="PLJ64_08910"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HNC93641.1"
                     /translation="MTGAPVTALIMAAGQGTRMRSALPKVLHPVCGRPMIAWPVLAAR
                     EAGADRVAVIVSPERDLSRALPDGVETVEQVESNGTGGAVRSALEIVRESPRVLVLSG
                     DVPLISAEVITGLLASQDESGASATVMTAILDDPGSYGRIVRGGDGTIRKIVEAKAEG
                     DATRE"
     gene            complement(703..1260)
                     /locus_tag="PLJ64_08915"
     CDS             complement(703..1260)
                     /locus_tag="PLJ64_08915"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HNC93642.1"
                     /translation="MEYNATPEKEVKRASGIFTGAGILMILVGCFAIVAPHIVGQAFA
                     LLVGISFVIASVFIFVWAFSAEGAGTVIARIAWALVALIVGLIIVTHTDLSAKAFTVI
                     LIAYFLAAGVVRLGAAFAQRGVPGAGWLGLNGALSLLIGLIFVASWPNSAEWAIGLLI
                     GIDLIFSGWTLILLSQEAKSQALGS"
     gene            1305..1376
                     /locus_tag="PLJ64_08920"
     tRNA            1305..1376
                     /locus_tag="PLJ64_08920"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1338..1340,aa:Gln,seq:ttg)
     gene            1473..3794
                     /locus_tag="PLJ64_08925"
     CDS             1473..3794
                     /locus_tag="PLJ64_08925"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HNC93643.1"
                     /translation="MRKLAVAVAVAVTTFFAQGIQAQAAVTDAFDGAITCTTQPAIPS
                     PLPGSEYAGQRWCGSNTSLPGLGSTVDSWDGTPIDVNFALPPEPGTGPDGNYPVVMIF
                     HGWGGMKSGFTDMQRWLSRGYAVFSMTDRGFHESCGSAAARLTGGTACDNGYIHLLDT
                     RYEVRDAQYLAGLLVDDGLISPTRIATTGGSYGGGLSMALAALKNRVMLPDGALTEWK
                     SPAGTDMSLAVAIPSIPWTDLAYSLVPNGNNLDYIEDASYYGRFGVMKESWVNLLYTL
                     GLGTGNYAAAGEDPGADLTGWKSLLDTGEPYDGQAGAQAILDEITAHHSSYYIDHSIQ
                     PAPLLIYSGFTDDLFPVDEATRFYNRTRAQYANAKIGLIFGPNSGHMRGMSKSDATAY
                     QQEVENQWVDFYLKDEGAEPASNVRALLQTCPANAPVGDPYVADDWASISPGEIRLVS
                     PESQTVSAGGGDPASAGIFNPAPTGQACSTAPGGKETGAASYELDPAPAGGFTVLGAP
                     TVVAKIQQEGVNSQIAARLVDVSPDGTTKLLVARGLWRPQGEGFQVFQLHANAWKVEE
                     GHVLRLELLPKDASVPAGLVANYGRPSNGQTDATVTDLDLRIPVADQPGAAGGLVTAP
                     AAKVLPDRPGVKLAPGYDTDDQVPIDDYEPPPVDHPKTIRKRIVIGKVQIKGKRVLIK
                     AGCPKAATVCPKARITVAGAKRKTRGTLARIKGVVVKPGENRLVSTRLTKRGQKVLKQ
                     TRGRKLAVKVTVKPAGMSKRTVRRVARVTRQRR"
     gene            3921..6248
                     /locus_tag="PLJ64_08930"
     CDS             3921..6248
                     /locus_tag="PLJ64_08930"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HNC93644.1"
                     /translation="MFVFLGVTGPAQAAAPASILDGQIPCGVVTDEGSGGSAVSTSLG
                     QVWCGTIRPSDNTGATVTPAIESVRSTAKTTDGVPLDINFAMPDPGTFGPPPYPTLMA
                     FHGYGGNRFSFRALQRWLDKGYATYSITARGFDESCLSAGSKAADPEGCAKGYIHLID
                     QRFEIRDTQNFIGNLVDEGLVQPDGLAAWGVSYGGGHTLSFAALKDRMMNPDGTLVPW
                     TSPDGTPISIKAAVAQVPPTDLPYILAPSGSDLDYIADSSYIYKTRDGRASRVGILKE
                     GWAQGLAATGFTAPIGTDPGADLQGWLADFEKGEPYDGKASIRNSIAELARYHSPYGI
                     DSSEAPAPMFISAGYNDDLVPAVEELRLYNRTRARYPDLPITLFLGSLGHPRGQIQAN
                     VSSALRTKEDQWTDYYLGGTGTRPASEVISYTQTCPNGGDGGGPYTASDWASLSPGEI
                     RLKDAAVQTIGAAGGDPAIAGAWNGVTAGQNPCGPESGSKEPGSANWDLAPAPAGGYT
                     MQGSPTVIADIDLGDSPNSEIAGRLVDLSPDGTTKILVARTIFRPWPSGVQVFQLHPN
                     AWKVEEGHALRLELLAKDAAGAAGSFLINSFRPADGQGDITVRDMELRIPVVETPGAL
                     GGLVKAPAKKVLPDRRGVKLAPGYAATGALAIDNPLRAGSKGSVRGKSLKIALTCPAS
                     YTNACPNKSVTIKGAPKKGRGRNVKVASASKVKVPGGKTKQVTMKLTAAARKLFRDLK
                     RGKRKVKGLKSLRVAILVDGRQSGYATVRRAGRVR"
     gene            6359..>7259
                     /locus_tag="PLJ64_08935"
     CDS             6359..>7259
                     /locus_tag="PLJ64_08935"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HNC93645.1"
                     /translation="MRKLVLLLALLGSLFAVSAAQAAPPASVFNGAVACTTQGVGDYE
                     GQTWCGSGQYNQNADIRSAVESFDGVPIDVNVAFPDHTQFGDGPYPLVYVFHGYGGGK
                     INFTGMQHWLDKGYAVFSQTNRGFHESCGTATSKADDADCVTKGFVRLDDTRYEVRDA
                     QLFAGMLVDEDLVQPNKIAATGGSYGGGMSMALAALKDRIMLPDGTYAAWTSPQGTPM
                     SIAVAIPDIPWTDLAYSLVPNGSNIDYIKDASYYGPFGIMKQSYVNGLYISGLGAPGY
                     YTDPGTQPEADLAGWKAFMDAGEP"
BASE COUNT         1331 a         2498 c         2321 g         1109 t
ORIGIN      
        1 cctcgcgggt agcgtccccc tcggccttcg cttcgacgat cttgcggatc gtgccgtcgc
       61 caccgcggac gatccggccg taggagccgg ggtcgtcgag gatcgcggtc atcaccgtgg
      121 cggaggctcc gctttcatcc tgggaggcga gcagcccggt aatcacctcg gccgaaatca
      181 ggggtacgtc gccggaaagc accagcaccc gcggggactc gcgaacgatc tcgagggcgg
      241 acctgaccgc gccgccggtt ccgttcgatt caacctgttc gaccgtttca accccgtccg
      301 gcagggcccg ggaaagatcc cgctcggggg aaacgatcac cgccacccgg tcggccccgg
      361 cttcgcgcgc ggcgagcacc ggccaggcga tcatcggccg gccacagacg ggatgaagca
      421 ccttgggcag ggcagaccgc atgcgggtgc cctgaccggc cgccatgatc agcgctgtta
      481 cgggggcccc ggtcacgagg gacattctaa gtcggcgagc ttcggcatgc cgacttgagt
      541 cggcatgcct cgccccgcct ccaagttaaa cccgccggcc catcaaacga attgatggtt
      601 gggccggctc agctagaggt tcttccggcc cgattatctg ggggtgatct gggttgaagt
      661 tattcgttcc atgtgcgaat aacttcaatg cagatgggag cgttagctgc cgagggcctg
      721 ggacttggct tcctgggaga ggaggatcag ggtccagccg gagaagatca ggtcgatgcc
      781 gatcaggagg ccgatcgccc actcggccga attcggccag gaggcgacga agatcaggcc
      841 gatcaggagc gaaagtgctc cgttcaagcc aagccagccc gccccgggca cgccccgctg
      901 ggcgaaagcg gctccgagcc ggacgacgcc ggccgcgagg aagtaggcga tcaggatcac
      961 ggtgaaggcc tttgcgctca ggtccgtatg ggtgacgatt atcaacccga ctatcaatgc
     1021 gaccagggcc caggcgatcc tcgcgatcac ggttccggct ccctcggccg agaaagccca
     1081 gacgaagatg aacaccgacg caatgacgaa agagatcccg accagcagcg caaatgcctg
     1141 accgacgatg tggggcgcga cgatcgcgaa acaaccgacc aggatcatca ggatcccggc
     1201 gccggtgaaa atcccggatg cgcgtttgac ttccttctcc ggtgttgcgt tgtattccat
     1261 ggccaaagcc tatcctcgcc agcggaggta gagtccggcg cgattccggg atggtgtaat
     1321 cggtagcaca ccggtttttg gtaccggcag tggaggttca agtcctcctc ccggagtttg
     1381 gagtacctct atgtttggga atcgcgggac gcggttatgg gtgcagggtc agtctcgttt
     1441 tcaggcaata gggacatcac ccggaggaat cgatgcgaaa gctcgcagtc gcagttgcgg
     1501 tagccgtcac caccttcttc gcccagggca ttcaggccca ggccgcggtc accgacgcct
     1561 tcgacggcgc gatcacctgc acgacccagc cggcgatccc ctctccgctg cccggcagcg
     1621 agtacgccgg acagcgctgg tgtggctcga acacttcgct gcccgggctt ggcagcaccg
     1681 ttgattcctg ggatggaacc ccgatcgacg tcaacttcgc cctgccgccc gaaccgggaa
     1741 ccgggccgga cggcaactac ccggtggtca tgatcttcca cggctggggc ggcatgaagt
     1801 ccggtttcac cgacatgcag cgctggctca gccgcggcta cgcggtcttt tcgatgaccg
     1861 accggggatt ccacgaatcc tgcggcagcg cggccgcccg cctgaccggc ggcaccgcct
     1921 gcgacaacgg atacatccac ctgctcgaca cccgctacga agtgcgcgac gcccagtacc
     1981 tggccggtct gctcgtcgat gacggcctga tctcgccgac ccggatcgcc accaccggcg
     2041 gctcctacgg cgggggtctc tcgatggccc tcgcggccct gaagaaccgg gtaatgctgc
     2101 cggacggcgc cctgaccgaa tggaagagcc cggccgggac ggacatgagc ctggccgtgg
     2161 cgatcccgag cattccctgg accgacctcg cctactcgct cgtcccgaac gggaacaatc
     2221 tcgactacat cgaggacgcg tcgtattacg ggcgcttcgg cgtgatgaag gagtcctggg
     2281 tcaacctgct ctacacgctc ggactgggaa ccgggaacta cgcggctgcc ggcgaggatc
     2341 ccggcgccga cctgaccggc tggaagagct tgctggacac cggcgagccg tatgacggcc
     2401 aggccggggc ccaggcgatc ctcgacgaga tcaccgctca ccactcctcg tattacatcg
     2461 accacagcat ccagcccgcc ccgctcctga tctactcggg cttcaccgac gacctcttcc
     2521 cggttgacga ggcaacccgc ttctacaacc ggacccgggc ccagtacgcg aacgcgaaga
     2581 tcgggctgat cttcggaccg aactcgggcc acatgcgggg catgagcaag tccgatgcga
     2641 ccgcctacca gcaggaagtg gagaaccagt gggtcgactt ctacctcaag gacgaaggcg
     2701 ccgaaccggc ctccaacgta cgggccctgc tccagacctg cccggcaaac gcgccggtcg
     2761 gtgaccccta tgtcgccgat gactgggctt cgatttcccc cggcgaaatc cggttggtct
     2821 caccggaatc ccagaccgtt tccgccggtg gcggcgatcc cgcgtccgcc gggatcttca
     2881 atcccgcccc gaccggccag gcctgctcga ccgcgcccgg cggcaaggaa accggggcgg
     2941 ccagctacga gctcgacccg gccccggccg gcggcttcac cgtgctcggc gctccgaccg
     3001 tggtcgcgaa gatccagcag gaaggcgtca actcgcagat cgcggcccgg ctggttgacg
     3061 tctcgcccga cgggacgacc aagctgctgg tcgcccgtgg cctctggcgg ccgcagggtg
     3121 agggtttcca ggtattccag ctccatgcca acgcctggaa ggtcgaggaa ggccacgtgc
     3181 tgcgactcga actgctgccg aaggacgcct cggtgccggc cggccttgtc gcgaactatg
     3241 gccgcccctc gaacggccag accgatgcca ccgtcaccga cctcgacctg cggatcccgg
     3301 tcgccgacca gcccggcgcg gccgggggtc tggtcaccgc gccggccgcc aaggtcctgc
     3361 ccgaccggcc cggcgtgaag cttgcccccg gctacgacac cgacgatcag gttccgatcg
     3421 acgattacga gccgccgccg gtcgaccacc cgaagacgat caggaaacgg atcgtgatcg
     3481 gcaaggttca gatcaagggc aagcgagtgc tgatcaaggc cggttgcccg aaggcagcaa
     3541 ccgtctgccc gaaggcgcgg atcaccgtgg ccggcgcaaa gcggaagacc cgcgggacac
     3601 tggccaggat caagggagtg gtcgtcaaac ccggcgaaaa ccggctggtt tcgacccgcc
     3661 tgaccaaacg cgggcagaag gtgctgaaac agaccagggg ccggaagctg gcggtgaagg
     3721 tcaccgtcaa accggccggg atgtcgaaga gaacggtgcg acgggtagcg agggtcaccc
     3781 gccagcgcag gtagggcccc cgcggaaatc tgccggcttg catatatctt tgatcagcgc
     3841 tacgatcccc gcggttcggc aagtggccgg gagtacgacc gggaggggtt gaagtggtgc
     3901 ggagagccgt tgttcttgca gtgttcgtgt tccttggcgt gaccggaccc gcgcaggcgg
     3961 cggcacccgc gagcatcctg gacgggcaga ttccctgtgg cgtcgtgacc gatgaaggtt
     4021 caggcggcag cgccgtcagc acctccctgg gacaggtctg gtgcggcacg atccgcccct
     4081 ccgacaacac cggcgcaacc gtcaccccag caatcgaatc ggtccgcagc accgcgaaga
     4141 ccaccgacgg cgtcccgctg gacatcaact tcgcaatgcc cgacccgggc actttcggcc
     4201 cgcccccgta ccccaccctg atggctttcc acggatacgg cggaaaccgc ttcagcttca
     4261 gggccctgca gcgctggctc gacaagggtt acgcgaccta ctcgatcacc gcccgcggct
     4321 tcgacgagtc ctgcctgagc gccggctcga aggccgccga cccggagggc tgcgccaagg
     4381 gttacatcca cctgatcgat cagcgcttcg agatccgcga tacccagaac ttcatcggca
     4441 acctggttga cgagggactg gtgcagccgg acggcctcgc tgcctggggc gtctcgtacg
     4501 gcggcgggca cacgctctcg ttcgccgcgt tgaaggaccg gatgatgaac ccggacggga
     4561 cgctggtccc ttggaccagc ccggacggca ccccgatcag catcaaggcc gccgtcgccc
     4621 aggttccccc gaccgatctg ccctacatcc tcgcgccgag cggttccgac ctcgactaca
     4681 tcgccgattc gagctacatc tacaagacga gggacggccg ggcgagccgg gtcgggatcc
     4741 tcaaggaagg ctgggcccag ggccttgccg caaccggttt caccgccccg atcggtaccg
     4801 atcccggcgc cgacctgcag ggctggctgg ccgactttga aaagggcgag ccatacgacg
     4861 ggaaggcttc gatccggaac tcgatcgccg aactcgccag gtaccactcg ccctacggaa
     4921 tcgacagcag cgaagccccg gcccccatgt tcatcagcgc cggttacaac gacgacctgg
     4981 ttccggcggt cgaggaactc cggctctaca accggacccg ggcccggtac ccggaccttc
     5041 cgatcaccct cttcctcggt tcgctcggcc acccgcgcgg ccagatccag gcgaacgtgt
     5101 caagtgcgct gcggaccaaa gaggaccagt ggaccgacta ctacctcggc ggcaccggaa
     5161 ccaggccggc gtcggaggtc atctcctaca cccagacctg ccccaacggc ggtgatggcg
     5221 gcgggcccta cacggcctcc gactgggctt ctctttcgcc cggcgagatc cggctcaagg
     5281 atgcggccgt gcagacgatc ggcgccgccg gaggggaccc ggcgatcgcc ggcgcctgga
     5341 acggtgtcac tgccgggcag aacccctgcg gccccgagtc cggcagcaag gaacccggat
     5401 cggccaactg ggatctcgcc ccggccccgg cgggcggtta cacgatgcag ggctccccga
     5461 cggtgatcgc cgacatcgac ctcggtgatt ccccgaactc ggagatcgcc ggtcgcctgg
     5521 tcgacctcag ccccgacgga accaccaaga tcctggtcgc gcggacgatc ttccgcccct
     5581 ggccgagcgg tgtccaggtc tttcagctcc accccaatgc ctggaaggtc gaggaaggcc
     5641 acgccctgcg acttgagctg ctggccaagg acgccgctgg agcggccgga tcctttttga
     5701 tcaactcctt ccgccctgcc gacgggcagg gtgacatcac ggtcagggac atggaactgc
     5761 ggatcccggt cgtcgagacc cctggcgcgc tcggcgggct ggtcaaggct ccggcgaaga
     5821 aggtcctgcc cgaccgccgg ggcgtgaagc tcgctcccgg ctacgcggcg accggcgccc
     5881 tggcgattga caacccgctc agggccggct cgaaggggtc ggtcaggggc aagtccctga
     5941 agatcgccct gacctgcccg gccagctaca ccaatgcctg cccgaacaaa agcgtgacca
     6001 tcaagggcgc gccgaagaag ggccggggca ggaacgtgaa ggtcgcgtcc gcgagcaagg
     6061 tgaaggtccc cggcggcaag acgaaacagg tcaccatgaa actgaccgcc gccgcacgca
     6121 aactgttccg cgacctcaag cgaggcaagc ggaaagtcaa gggactgaag tcgcttcgcg
     6181 tcgcgatcct ggtcgacggc aggcagtcgg gctacgcaac ggtgagaaga gcgggcaggg
     6241 tccggtaaac gcgggcggta ctgccgttcg caacttgtcg cgtaccaacg cgttctatag
     6301 agtgactccg cttcgggatt ggcccggggc agggaaaaca ggagagaacc ggagaaccat
     6361 gcgtaagctt gtattgctgc tcgccttgct gggcagcctc tttgcagttt cggccgccca
     6421 ggcggcaccg cccgcgtccg tcttcaacgg cgccgtcgcc tgcacaaccc agggcgtcgg
     6481 tgactacgaa ggccagacct ggtgcggcag cggccagtac aaccagaacg ccgacatccg
     6541 cagcgccgtc gagtccttcg acggggttcc gatcgacgtc aacgtcgcct tccccgacca
     6601 cacccagttt ggcgacggcc cctacccgct ggtctacgtc ttccacggct acggtggcgg
     6661 caagatcaac ttcacgggca tgcagcactg gctcgacaag ggctacgcag tcttctccca
     6721 gaccaaccgc ggcttccatg agtcctgcgg taccgccacc tcgaaggccg atgacgccga
     6781 ttgcgtgacc aagggcttcg tgcgtctcga cgacacccgt tacgaagtcc gcgacgcgca
     6841 gctcttcgcc ggcatgctgg tcgacgagga cctggtccag ccgaacaaga tcgccgcgac
     6901 cggcggcagc tacggcggcg gcatgtcgat ggccctggcg gcgctgaagg accgcatcat
     6961 gctcccggac ggcacctacg ccgcctggac cagcccgcag ggcactccga tgagcatcgc
     7021 ggttgcgatt ccggacatcc cgtggaccga ccttgcctac tccctggttc ccaacggcag
     7081 caacatcgac tacatcaagg acgcctccta ctacggcccg ttcggcatca tgaagcagtc
     7141 ctacgtgaat ggcctctaca tcagcggcct tggcgccccc ggctactaca ccgatcccgg
     7201 cacgcagccg gaagcggacc tggccggctg gaaggccttc atggacgccg gagagccgt
//
LOCUS       DAOQKN010000131         7223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_5097_length_7223_cov_6.371512, whole genome shotgun sequence.
ACCESSION   DAOQKN010000131 DAOQKN010000000
VERSION     DAOQKN010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 7223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7223
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5097_length_7223_cov_6.371512"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /locus_tag="PLJ64_08940"
     CDS             <1..875
                     /locus_tag="PLJ64_08940"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HNC93646.1"
                     /translation="ITGMNPDEELPPEIAARGYYNQPVWKRIVVIGAGPAVNIVLAFL
                     ILWVLAFGIQQPSQNVEQIQPGSPAAAKLQAGDRIISVDGVSGSQEKIADEIAAHQCP
                     GKQTDGCKAAEPATVVVMRDGQEKTLRITPVYDASVQRSRLGFAFGLEDANPGPVEAA
                     RISADYMWFVTSSTVSALSRLYEKEQRDQLSGVVGSYEVTRQAVDVGAREALTLIALI
                     SLSLGIINLFPFLPLDGGHIFWSLVEKVRGRPVPFAVMERAGLIGFALVIGLFILGLN
                     NDIGRLTGEGFNVR"
     gene            1054..2862
                     /locus_tag="PLJ64_08945"
     CDS             1054..2862
                     /locus_tag="PLJ64_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HNC93647.1"
                     /translation="MQTSETVTEFDGGPAVEPEFKNLYEAFQATVARKGDGLAIKSEA
                     EEVELSWNDLDKRVRAIAGGLHALGIQRGDRVGMLLGARSDFIPIDLAVVSIGGLPFS
                     IYQTLAPDQIEYVVEDSGTELIITEEAYLPNLLEARKNLPNLKRVVMLDGEGGDMTMA
                     ELEAMDPEFDPAPLAAEVGLDDPLTLIYTSGTTGPPKGVLLTQGNLMSMLTTVMHSVL
                     EIPESGRVISWLPAAHIAERGANYYTPVMHGLEVHVCPDPKRIIEFLPKVRPSWFFAV
                     PRIWEKLKAGLESNLASLPEEQREKAQAGLQAAIQKVRLEQAGEEVPEELAAAVAAAD
                     EALFSHLRVALGFDEALAVSAGAAPTPAEVLEFFHAIGIPVGELWGLSETCGVITINP
                     PKKIKIGTVGPPVPGVEMRVAEDGELLCRSPFVMKEYRNKPEKTAETIVDGWLLTGDI
                     GEIDEEGYVTILDRKKELIINAAGKNMSPANIEAQIKVSSPLINQAVTIGDSRPYNVA
                     LIVLDPDFAPVWASQNGIEGSFEDLAGDERIEAAIQAAVEEANGKLARVEQIKKFKLI
                     PGEWLPGGDELTPTMKLKRKPIAEKYESEIEDLYST"
     gene            3070..4020
                     /locus_tag="PLJ64_08950"
     CDS             3070..4020
                     /locus_tag="PLJ64_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93648.1"
                     /translation="MEDETAPATTLQAGIVVLLGLLRAEEAETDQPFSTGALRTRILG
                     DLSGPDVTAGELGLALWAESRCGGEAIGEIDGLIRRATVGGLEKVPTEQIAWLASGLA
                     ETAVLTGDPSTGLIREVKAELSARITPGGLARDVHARRLGVAVPVTGQFHLLHALVQL
                     VRAGEEDCRNRADQLAGALLALQREDGAWPGLIDPVRSEAAVVYPVLAVTQIAAAPIA
                     LRAATELGLGDFEAAAKSGIQWANGGNALGLSLVHEADARIDRGILTKRKPGALERGI
                     SSAARRIRGQAFEPDAKQLIIDPAASSEDLGWVLEAWAGR"
     gene            4099..5355
                     /gene="ispG"
                     /locus_tag="PLJ64_08955"
                     /gene_synonym="gcpE"
     CDS             4099..5355
                     /gene="ispG"
                     /locus_tag="PLJ64_08955"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HNC93649.1"
                     /translation="MASKRQIFVGRVPVGGGAPVAVQTMTKTETANLEATMEQINTVA
                     EAGADIVRVAVPRDKDIEALKTIVKQSPIPIIADIHFNHTLALKAIEAGADCIRLNPG
                     NIGGREKVAEVAALANKRGTPMRIGVNSGSLPKHLHALEREDPVEALVTAAVEFVDLM
                     EGLDFTNFKVSIKSTNVPNTIAANRLLSARIPYPIHLGITEAGTKWSGSLKSAVGLGA
                     LLADDIGDTIRISLSTFHAEEEVKVAWEILKALKLREKGPVLIACPTCGRLQFDMDAV
                     VAEVEARLETYDEPIEVAVLGCAVNGIGEAKGADFGIAGAKNEGVVFAKGKAIKKVPT
                     SQLVETLFEQINLSLERDGKVEFDEKESAEGEAWVKKIEDENAGDLTPEKIAKMEHEA
                     SQKAENDKLLVDEEVSPTAGRRFTRA"
     gene            5448..6323
                     /locus_tag="PLJ64_08960"
     CDS             5448..6323
                     /locus_tag="PLJ64_08960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC93650.1"
                     /translation="MPEGPSKTAEYVATFRAVESARPEGERLFSDPYAASFLDGKLRL
                     GAALARNGMGQRLVCRYIDRNWPGSRPSAVIRTRLIDDAVTAAIEGGAEQLAILGAGY
                     DTRGLRLPAAREIPGFELDQPPTQERKKQRLRARDGDLPSNVTYVPFDLLDEGVGAPL
                     ERAGFRTGLKSVYIWEGVLSYLTPEAVDQTLAWITASGAPGSRLVYTYVDVALLKQGG
                     EDEPPWSSQVAKVGEPFRYGLDPAKMDAFLGDRGLKPVWDQSTLEAIAQHAVPGGRNA
                     PGFYRVAEAEVLPDR"
     gene            6336..>7223
                     /locus_tag="PLJ64_08965"
     CDS             6336..>7223
                     /locus_tag="PLJ64_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HNC93651.1"
                     /translation="MTRLSRTLLPTLKDPPADAEAVSHKLLVRAGMIRQVGAGLWTYL
                     PAGLRSHRKAEQIIREEMNRIGGQEISMPILQPADAWQKTGRYGIEEMFKLEDRKGSP
                     MVLAMTAEECVTGHVSREVRSYRDLPQLLYQIQTKERDEPRPRAGVLRTREFMMKDAY
                     SFDRDEAGLDETYNLCIEAYDRIYDRSGLRWYRVESDVGMMGGSGAHEYMAPCGAGED
                     TIALASGYAANLEVASANPRPVQGGEELPAPVEVETPGLKTIEQVSGSLGLDPGALIK
                     SVALVTDEGEFVLALIRGDH"
BASE COUNT         1380 a         2276 c         2420 g         1147 t
ORIGIN      
        1 ggatcaccgg catgaacccg gacgaggaac tgcccccgga aatcgccgcc cgcggctatt
       61 acaaccagcc ggtctggaag cggatcgtgg tgatcggggc cgggccggcg gtcaacatcg
      121 tgctcgcctt cctgatcctc tgggtgcttg ccttcggcat ccagcaaccg agccagaacg
      181 tcgaacagat ccagcccggc agcccggccg cggcgaagct ccaggccggg gaccggatca
      241 tctcggttga cggggtgagc ggaagccagg agaagatcgc cgacgagatc gccgcccacc
      301 agtgtcccgg caagcagacc gacggctgca aggcggcgga gccggccacc gtcgtggtca
      361 tgcgcgacgg ccaggagaag acgctccgga tcacaccggt gtatgacgcc tcggttcagc
      421 gcagtcgtct cggcttcgcc ttcggtctcg aagatgccaa ccccggcccg gtcgaggcgg
      481 cgcgaatctc ggccgactac atgtggttcg taacctccag cacggtttcg gcgctgtccc
      541 gcctttacga gaaggagcag cgagaccagc tttccggggt ggtcggcagc tacgaagtga
      601 ccaggcaggc cgtcgacgtc ggggcccgcg aggcgctgac cctgatcgcc ctgatcagcc
      661 tctcgctcgg catcatcaat ctcttcccgt tcctgccgct cgacggcggc cacatcttct
      721 ggagcctggt cgagaaggta agggggaggc cggtcccgtt cgcggtcatg gaaagagccg
      781 gcctgatcgg cttcgccctg gtcatcggcc tcttcatcct cggcctcaac aacgacatcg
      841 gccgcctgac gggcgagggg ttcaacgtta gataaggagt cgggcggggt cggacccgcc
      901 ctccatgtag aacccgctcg cccacggagg caacggatag atgggcgagc tccgttggtt
      961 ggttggccta gggggttgcc cctatatgcc cgttgctctg gttgggatac attcccggcg
     1021 tagctttttg ggaagagagc gaaggaggag caggtgcaga cgtccgagac ggtgaccgaa
     1081 tttgacggtg gccccgcggt tgaaccagaa ttcaagaacc tctacgaggc ctttcaggcg
     1141 accgtcgccc gcaagggtga cgggctggcc atcaagtccg aggccgaaga ggttgaactc
     1201 agctggaacg accttgacaa gcgggttcgc gcgatcgccg gcggtctcca cgcgctcggc
     1261 atccagcgcg gcgaccgggt cggaatgctg ctgggagcaa ggtccgactt cattccgatc
     1321 gatctcgcgg tggtttcgat cggcggcctg cccttctcga tctaccagac cctcgccccc
     1381 gaccagatcg aatacgtggt cgaggattcc gggaccgagc tgatcatcac cgaggaggcc
     1441 tacctgccca acctgctcga ggcccgcaag aacctgccga acctgaagcg cgtcgtgatg
     1501 ctcgacggtg aaggtggcga catgaccatg gcggaactgg aagccatgga tcccgaattc
     1561 gatccggccc cgctggcggc cgaggtcggg ctcgacgacc cgctcactct gatctacacc
     1621 tccgggacca ccggtccgcc caagggtgtg ctgctgaccc agggcaacct gatgtcgatg
     1681 ctgaccacgg tcatgcattc ggtgctcgag atccccgaaa gcggccgggt gatttcctgg
     1741 ctgccggccg cccacatcgc cgaacggggc gccaactact acacgccggt catgcacggt
     1801 ctcgaggtcc acgtctgccc ggacccgaag cggatcatcg agttcctgcc caaggtgcgg
     1861 ccctcctggt tctttgccgt cccccggatc tgggagaagc tgaaagccgg actggaatca
     1921 aatctggcct ccttgccgga agagcagcgc gaaaaggccc aggccggact tcaggcggcg
     1981 atccagaagg tacggctgga acaggccggg gaagaggtgc cggaagagct ggccgccgcg
     2041 gtcgcagcgg ccgacgaggc gctcttctcg cacctgcggg tcgccctagg cttcgacgag
     2101 gcgctggccg taagtgccgg agctgcaccg accccggccg aggtgctcga gttcttccat
     2161 gcgatcggca tcccggtcgg cgagctctgg ggcctctccg agacctgcgg cgtgatcacg
     2221 atcaacccgc cgaaaaagat caagatcggc acggtcggac caccagttcc cggggtcgag
     2281 atgcgggtgg ccgaggatgg cgagctgctc tgccgcagcc ccttcgtgat gaaggagtac
     2341 cgcaacaagc ccgagaagac ggccgagacg atcgtcgatg gctggctttt gaccggtgac
     2401 atcggcgaga tcgacgaaga gggttacgtg acgatcctcg accgcaagaa ggagctgatc
     2461 atcaacgcgg ccggcaagaa catgtcaccg gccaacatcg aggcgcagat caaggtctct
     2521 tcacccctga tcaaccaggc ggtgacgatc ggagacagcc gcccctacaa cgtcgcgctg
     2581 atcgtgctcg accccgactt cgctccggtc tgggcctccc agaacgggat cgagggttcc
     2641 ttcgaggacc tggccggtga cgagcggatc gaagcggcca tccaggccgc ggtcgaggaa
     2701 gccaacggca aactggcccg ggtcgagcag atcaagaagt tcaagctgat cccgggtgaa
     2761 tggttgccgg gtggcgatga actgacgccg accatgaagt tgaagcggaa accgatcgcc
     2821 gagaagtacg aatccgagat cgaggatctc tactccacct gagagtctcc gggaccggta
     2881 ccgataggcc ccttcctcgg cccgaaacgt cgtcagggac ctatcggcac cggtccagat
     2941 gcttcgagcg catataacct cggcttatag atgatcgaac cgcgcgccct ggagcgcatc
     3001 gcacttacaa ggttgcctga gctgcaggtt gcttccgggc tttttcgggc tactgcgcgg
     3061 attgaggggg tggaggatga gacggctccg gcgacgacgc ttcaggccgg gatcgtggtg
     3121 ctcctcgggc tgttgcgggc cgaagaggcc gagaccgacc aacccttttc caccggcgcg
     3181 ctgaggaccc gaatcctagg tgacctgagc ggtccggacg tgaccgccgg tgaactcgga
     3241 ctcgcgctct gggccgaatc gaggtgcggc ggagaagcga tcggggaaat cgatggcttg
     3301 atccggcggg caacggtcgg aggcctggag aaggttccga ccgagcagat cgcctggctt
     3361 gcctccggtc tggccgagac cgcggtgctg accggcgacc cgtcgaccgg actgatcagg
     3421 gaagtcaagg ccgaactgag cgcccggatc acgcccggcg gcctcgccag ggacgtccac
     3481 gcccgaaggc tgggagtcgc agtcccggtc accggccagt ttcacctgct ccacgccctg
     3541 gttcagctgg tcagggcggg cgaggaagac tgccggaaca gggccgatca gctggctggg
     3601 gcgttgcttg ccctccagcg cgaggatggc gcatggcccg gtttgatcga tcccgtccgt
     3661 tctgaagctg ccgtcgtgta tccggtcctg gcagtcaccc agatcgcggc cgcaccgatc
     3721 gcgctccggg ctgccacgga actcgggctg ggcgactttg aagcggcggc aaaatccggc
     3781 attcagtggg ccaacggagg caacgcactc gggctctccc tggtccatga agcggatgcg
     3841 cggatcgacc ggggcatcct gaccaaacga aagccgggcg ctctggaacg cggaatcagt
     3901 tccgctgcga ggcgaatccg gggtcaggca ttcgagcccg acgccaaaca actgatcatc
     3961 gatccggccg cctcgagcga ggacctcggg tgggtcctcg aagcctgggc cggcaggtag
     4021 ccaccacaaa aggccgctgg cgccccgccg cgggcgatcg ccttgtctgc ggcccccgat
     4081 cggtagcctg caggggtagt ggcatcgaaa agacagatat tcgtcggccg ggtaccggtg
     4141 ggcggcggag cgccggtcgc ggtccagaca atgaccaaga ccgagaccgc caacctcgaa
     4201 gcgacgatgg agcagatcaa cacggtcgcc gaagccggcg ccgacatcgt tcgggtcgcg
     4261 gttccccgtg acaaggacat cgaggcgctg aagacgatcg tcaagcagtc gccgatcccg
     4321 atcatcgccg acattcactt caaccacacc ctggccctga aggcaattga ggccggggca
     4381 gactgcatcc gcctgaaccc gggcaacatc ggcggccggg aaaaggttgc cgaggtcgcc
     4441 gcgctggcca acaaacgggg cacaccgatg cgaatcggcg tcaattccgg ttcgctgccg
     4501 aagcacctcc acgcactgga gcgcgaagac ccggtcgagg cgctggtcac cgcggcggtc
     4561 gaattcgtgg acctgatgga aggcctcgac ttcaccaact tcaaggtttc gatcaagtcg
     4621 accaacgtgc cgaacacgat tgccgccaac cggctgctct cggcgaggat cccctacccg
     4681 atccacctcg gcatcaccga ggccggcacc aagtggtccg gttcgctgaa gagcgcggtc
     4741 ggtctgggtg cgctgctcgc cgatgacatc ggtgacacga tccggatcag cctctcgacc
     4801 ttccacgccg aagaagaagt caaggtcgcc tgggagatcc tcaaggcgct caagctgcga
     4861 gagaagggcc cggtgctgat cgcctgcccg acctgcggcc ggctccagtt cgacatggac
     4921 gccgtggtcg ccgaagtcga ggcaagactc gagacctacg acgagccaat cgaggtagcc
     4981 gtgctcggct gcgcggtcaa cggaatcggc gaggccaagg gtgccgattt cggaattgcc
     5041 ggagccaaga acgagggtgt cgtcttcgcc aagggcaagg cgatcaaaaa ggtgccgact
     5101 tcacagctcg tcgaaacgct cttcgagcag atcaatcttt cgcttgaacg cgacggcaag
     5161 gtcgagttcg acgagaagga atcggccgaa ggcgaagcct gggtcaagaa gatcgaggat
     5221 gagaacgccg gcgatctgac cccggagaag atcgcgaaga tggagcatga ggcttcgcag
     5281 aaggcggaga acgacaagct tctggttgac gaagaggtat caccgaccgc cggtcgtcgt
     5341 ttcacccgag cctgatgctg ggggcggggg ctggcagaga ttgcctcctc ggcccgaaac
     5401 gtcgtcggca ccctctgcca gtccccgccc gaccggaatt gcggtagatg ccggagggtc
     5461 cttccaagac tgctgaatac gtcgccacgt ttcgcgcggt ggaatcggcg cggcccgaag
     5521 gggagaggct tttcagtgac ccctacgcag cgagctttct tgacgggaag ttgaggttgg
     5581 gggcggcttt ggcccggaac gggatggggc agaggctggt ttgccgctat atcgaccgga
     5641 actggcctgg ttcgcggcct tcggcggtga tccgtacccg gctgatcgac gatgcggtca
     5701 ccgcggcgat cgagggaggg gcggagcagc tggcgattct cggggccgga tacgacacca
     5761 ggggtttgcg gctgccggcg gccagggaga taccgggctt cgagctcgac caacccccga
     5821 cgcaggagcg caagaagcag agactgagag cacgggacgg cgatcttccg tcgaacgtga
     5881 cctacgtgcc ttttgatctg ctcgacgagg gggtcggcgc accactggaa cgggcagggt
     5941 tcaggaccgg cctgaagtcg gtctacatct gggagggggt actgagctac ctgacaccgg
     6001 aagcggtgga ccagaccctg gcctggatca cggcctcggg agcacccgga agcaggctcg
     6061 tctacaccta tgtcgacgtt gccctcctca agcagggagg cgaggacgag cccccgtgga
     6121 gcagtcaggt ggcaaaggtg ggggagccgt ttcgctacgg cctggatccg gcgaagatgg
     6181 atgcctttct gggcgaccga ggcctgaaac cggtctggga ccaatcgacc ctggaggcga
     6241 tcgcacagca cgcggtgccg ggtggccgga acgccccggg cttctaccgg gtcgcagagg
     6301 ccgaagtcct tcccgacaga taggctccga cctcgatgac gaggctctcg cgcacacttc
     6361 tgcctaccct gaaggatccg cccgccgacg ccgaagcggt gagccacaag ctgctggttc
     6421 gggcgggaat gatccggcag gtcggcgccg gcctctggac ctatctgccg gccgggcttc
     6481 gctcccaccg caaggccgag cagatcatcc gtgaggagat gaaccggatc ggcggtcagg
     6541 aaatctcgat gccgatcctg cagccggccg acgcatggca gaagaccggt cgctacggga
     6601 tcgaagagat gttcaaactc gaggaccgca agggatcgcc gatggtcctc gcgatgaccg
     6661 ccgaggaatg cgtgaccggc catgtctcgc gcgaggtccg ctcctaccgc gacctgcccc
     6721 agctgctcta ccagatccag accaaggaac gggatgaacc gcgcccccgc gccggggtgc
     6781 tccggacccg ggaattcatg atgaaggacg cctacagctt cgaccgcgac gaggccggcc
     6841 tggacgagac atacaacctc tgcatcgagg catacgaccg gatctatgac cggtccggcc
     6901 tgcgctggta ccgggtcgaa agcgacgtcg gaatgatggg cggctccgga gcgcatgagt
     6961 acatggcacc ctgcggggcc ggcgaagaca cgatcgccct ggcctccggg tacgcggcca
     7021 atctcgaggt cgcatccgcc aatccgcgtc cggttcaggg cggcgaggaa ctgcccgccc
     7081 cggtcgaggt tgaaactccc ggcctgaaga cgatcgagca ggtttccgga tcgctcgggc
     7141 tcgaccccgg cgccctgatc aagtccgtcg ccctggtgac cgacgagggt gaattcgtgc
     7201 ttgccctgat ccggggcgat cat
//
LOCUS       DAOQKN010000132         7191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_5137_length_7191_cov_6.171945, whole genome shotgun sequence.
ACCESSION   DAOQKN010000132 DAOQKN010000000
VERSION     DAOQKN010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 7191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7191
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5137_length_7191_cov_6.171945"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1019
                     /locus_tag="PLJ64_08970"
     CDS             <1..1019
                     /locus_tag="PLJ64_08970"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNC93652.1"
                     /translation="LALAESEGTPFSEDPSLHWHPSAAAIAQLHSEGKVTVFPAVGYD
                     GANQSHFTSRHFWEVGELETDTRTGWMGRYIDLAGSDNNPLQGLSLSNTLAPALATHD
                     KPVAAVEGVTDYDLWSWTDAPVSDEMFKSFKRMGSMKSESPALRDSRKAIRATNIIRE
                     SLESFGDFESPVTYPDARLGQQLSGLAALLGAGMPLHCVSVSADGAYDTHSDQKQEFD
                     GAVKATSDSILAFQRDLEARGLQDRVLMQVWSEFGRRPEENGSAGTDHGAAGVAMIIG
                     SKARGQMVGEFPGLVNLDEDDNLRHTSDFRAMYCSLLEDWFGHDAAPIIPGAANFARP
                     VLLK"
     gene            1016..1555
                     /locus_tag="PLJ64_08975"
     CDS             1016..1555
                     /locus_tag="PLJ64_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93653.1"
                     /translation="MKRLLVLTLLLAATAHWFAIPAGAAGHGAAGSPAGGKASAAANQ
                     VCAWKWKRKKVVRWVIRHGRKKRIVTWRKVKVKVCREIPPPAPARLGVKAWEFGFTLS
                     AKEVAAGDTIVELNNQGEDDHNLHVQRTDGGEELATPDISPGGVERIRFTTAPGTYRL
                     WCSLPTHAERGMDTTFTAQ"
     gene            complement(1578..2957)
                     /locus_tag="PLJ64_08980"
     CDS             complement(1578..2957)
                     /locus_tag="PLJ64_08980"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNC93654.1"
                     /translation="MAWNIVIVGGGFAGANVARHLEKVLPRQSSRLTLVNDVNFLLYT
                     PLLPEAAAGTLEPRHVVTPLREILHRTNLRLGVVDAHDREAREVTLRSHMGQTERIPY
                     DHLVLAPGSISRFLPVPGLSEHAVGFKSLADAIFLRNHLIETLEMANATDDRQLREEL
                     LTYVFVGGGYAGLEALAELQDFAADAMRDYPKARLHGMRWILVEAADRVLPEIDQELA
                     DYAVDELRGRGIDIKLGTTLDEVTETTATISTGETVPTRTVVWTAGVVPNPSLQNFGV
                     PLDERGRVIVDSSMAVEGNEGIWSLGDSASVPNPKGGVCPPTAQHAVRQAPVVAANIA
                     AAIGLGERKIFDYRGNASFVNLGRYKAVGKIGDRTFRGFKAWWMARSYHMSQIPGASR
                     KARAVIDWTASLPFSRDISEVGSIGRPGVLNPDRYLHGGSHRPVEGDLPAAGRDRAEA
                     DAGSAGETA"
     gene            3030..4307
                     /locus_tag="PLJ64_08985"
     CDS             3030..4307
                     /locus_tag="PLJ64_08985"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HNC93655.1"
                     /translation="MGLTSRARGGLAGALALTAALIAVSIVSSSTASAATIVNEGAPP
                     PKISKVFPNRSSVVSETIANPASADDYWTPERLASAVPAEMPNPSDALDIPDFASGAS
                     ASADGGDFTPGNVTAFPQRVHGKIFFSVGVNNFSCSGTLIDSANQSVVFTAGHCVFDQ
                     ETHGFVSNLIFLPGYENGVAQLGAANGASVFTTSQWAQSGANAYDMGVVVLDQPFEKF
                     YGSRQIAFDLNPKGLNYTIYGYPSKPNPPYNGEVLQGCHSGFAGRDDGNGNITPYPVA
                     ASPCFMQQGASGGGWVVGGNYVNSVVSYGYCDSVPSTCGFIFGPYFSNAAKSLYVQAG
                     GSPAPTIKLKSGPKGVVRKRTVNFQFTGSAATLLGFRCRFDRQKEVACSSKISIRRLS
                     PGKHILRVWAVDQTGHKSKKPAVRSFRVVLPRR"
     gene            complement(4318..5754)
                     /gene="gatB"
                     /locus_tag="PLJ64_08990"
     CDS             complement(4318..5754)
                     /gene="gatB"
                     /locus_tag="PLJ64_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNC93656.1"
                     /translation="MSTEYEAVIGLEIHVQLSTRTKMFCGCELSFGDDPNVHTCPVCL
                     ALPGVLPVPNAQAIEYALQIGAALGCELSPVSTFDRKNYFYPDNPKAYQITQAARPFA
                     VNGRLGEVRIHHAHLEEDAAKTIHTGGGGRIKGSDASEVDFNRGGTPLVEIVTEPDLR
                     SGAQVREWGQLLRATIKQLGVSDVNMEEGSLRCDANVSIRPVGSTEFGTKTEMKNMNS
                     FRFIERGIEAEVARQKEVVASGGQVVQETLHFDPATGELHSLRSKEEAHDYRYLPEPD
                     LVPVVPTPAMLEEAASSLPELPAARAARYEGIGVSEQAALLLSFELEWAGYFEGVLAA
                     DQSIEPRVAANWVTGEFAAELRKQDDQALADSKVGPESLATLIGLVQAKKISHGAGKT
                     VLEEMVASGTDPVTVVAEKGLEQIADSGELEKIVIQAIEDNPKPAEQVREGNEKAIGA
                     LVGAVMKATQGRADGGEVNRLLRQHLGL"
     gene            complement(5751..7190)
                     /gene="gatA"
                     /locus_tag="PLJ64_08995"
     CDS             complement(5751..7190)
                     /gene="gatA"
                     /locus_tag="PLJ64_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HNC93657.1"
                     /translation="MAASGAETAAKIKSGELSADEVLAFWLDRAGRDRLNGYLWQADR
                     DSARQGLGEGGPLDGVPLAIKDIFCTEGVPTTAASRILGGYVPPYTATSVRKAMAAGM
                     PMLGKTNMDEFAMGSSNENSAFGACLNPWDTERVPGGSSGGSAAVVAGGLAPWSLGTD
                     TGGSIRQPAGFCGIVGLKPTYGAISRFGMIAFASSLDQCGPLTRNVTDAALLLNVLQG
                     RDEMDSTSVGIEGGIKAPDAERLDGMTFGVPRALMGEGIEAGVLARFEDTLIRIAELG
                     GEIVDIELPHAGHAISAYYVLAPAEASTNLSRYDGVRYGLRASNEDLLSMYEVTREDG
                     FGPEVKRRIMLGTYALSSGYYDAYYGRAQRVRTRIVEDFEKAFGEVDFVVTPTSPTVA
                     FKLGEKTSDPWTMYLSDVFTVPISLAGVPAISIPAGLAEPDGGGSELPVGFQIAAPAF
                     AEQKLLEASYALEQAIGFDGSAALEGGVS"
BASE COUNT         1207 a         2378 c         2362 g         1244 t
ORIGIN      
        1 tcctggccct ggccgaaagc gagggcaccc cgttttccga ggacccgagc ctccattggc
       61 atccttcggc ggcggcgatc gcccagcttc actccgaagg caaggtgacc gtcttcccgg
      121 ccgtcggtta cgacggagcc aaccagtccc acttcacctc tcgtcacttc tgggaggtcg
      181 gcgaactgga aaccgatacc cgcaccggct ggatgggccg ctacatcgac ctggccggca
      241 gcgacaacaa cccgctgcag ggcctttcgc ttagcaacac gctcgccccc gccctggcca
      301 cgcacgacaa gccggtcgcc gcggtcgagg gagtcaccga ctacgacctc tggtcctgga
      361 ccgacgcgcc ggtcagcgac gagatgttca agagcttcaa gcggatgggc tcgatgaaat
      421 cggaatcgcc ggccctgcgg gacagccgca aggcgatccg cgcgaccaac atcatccgcg
      481 aatcgctgga atcgttcggt gacttcgaat cgccggtcac ctatcccgac gcgcggctcg
      541 gccagcagct gtcggggctg gctgcattgc tcggggccgg catgccgctc cactgcgttt
      601 cggtctccgc ggacggcgcc tatgacaccc actccgacca gaagcaggaa ttcgacggtg
      661 ccgtcaaggc aaccagcgac tcgatcctgg ccttccagcg tgatctcgag gcacgcggcc
      721 tccaggacag ggtcctgatg caggtctggt ccgaattcgg acggcggccg gaagagaacg
      781 gttcggccgg gaccgaccac ggggcggccg gggtggcaat gatcatcggc agcaaggcca
      841 gggggcagat ggtcggcgag tttcccggac tggtcaacct cgacgaggac gacaaccttc
      901 gccacacgtc ggatttccgc gcgatgtact gctcgctgct cgaagactgg ttcgggcacg
      961 acgcagcgcc gatcatcccc ggcgcggcga acttcgcccg gccggtgctg ctcaaatgaa
     1021 acgcctgctg gtcctgacct tgctgctggc ggccactgcg cactggttcg ctatcccggc
     1081 cggggccgcc ggtcacggcg cagccggttc cccggccggc ggaaaggcca gcgccgcggc
     1141 caaccaggtc tgcgcctgga agtggaagcg caagaaggtg gtccggtggg tgattcgcca
     1201 cggcaggaag aagcgcatcg tcacctggcg caaggtcaag gtgaaggtct gccgggagat
     1261 tcctccgccc gcgccggccc ggctcggggt caaagcctgg gagttcggct tcaccctctc
     1321 ggccaaggaa gtcgccgccg gtgacacgat cgtggagctg aacaaccagg gcgaagacga
     1381 ccacaacctt cacgttcagc gaaccgacgg cggtgaggaa ctcgcgaccc cggacatcag
     1441 cccgggcggc gtcgaacgga tccggttcac gacggcaccg gggacctacc gcctctggtg
     1501 ctcgctgccg acccacgccg aacgcggcat ggatacgacg ttcaccgccc agtaggaacc
     1561 ggaccaggcc cttcggttca ggcggtttcg ccggccgacc cggcgtcggc ttcggcccgg
     1621 tcccggccgg ccgccggcag atcaccctcg accgggcgat gggagccgcc gtggaggtag
     1681 cgatcggggt tcagcacccc gggccggccg atcgagccga cttcggagat gtcgcgggag
     1741 aagggcaggc tggcggtcca gtcgatcacc gctcgtgcct tcctcgatgc gcccgggatc
     1801 tggctcatgt ggtacgagcg ggccatccac catgccttga agccgcggaa ggtgcgatcg
     1861 ccgatcttgc cgaccgcctt gtagcggccg aggttgacga aggacgcatt gccccggtaa
     1921 tcgaagatct tccgttcccc cagtccgatt gccgccgcga tgttggcggc gaccaccggc
     1981 gcctggcgca ccgcgtgctg ggccgtcggc gggcagacgc cgcccttcgg gttcgggacc
     2041 gaggcggaat ccccgagcga ccagattccc tcgttgccct ccaccgccat cgagctgtcg
     2101 acgattacgc ggccccgctc gtcgagcggc acgccgaagt tctggaggct cggattgggg
     2161 acgacgcccg cggtccagac cacggtccgg gtcgggacgg tctcaccggt cgagatggtc
     2221 gccgtggttt ccgtgacctc gtcgagggtc gtgccgagct tgatgtcgat gccgcggccg
     2281 cgcagttcgt ccaccgcgta atcggccagc tcctggtcga tttcgggcag cacgcgatcg
     2341 gcggcctcga ccaggatcca gcgcatcccg tgcagtcgtg ccttggggta gtcgcgcatc
     2401 gcgtcggcgg caaagtcctg gagttcggcc agcgcttcca gcccagcgta tccgcctccg
     2461 acgaagacgt aggtaaggag ttcttcccgc agttgccggt catcggtggc gttggccatc
     2521 tcgagggttt cgatcaggtg attgcgcagg aagatcgcat cggccaggct cttgaagccg
     2581 accgcatgct cgctcaggcc ggggaccggc aggaagcgcg agatcgagcc gggagccagc
     2641 acgaggtggt cgtagggaat gcgttcggtc tggcccatgt ggcttcgcag ggtcacttcg
     2701 cgggcttccc ggtcatgggc gtcaaccact ccgagcctca ggttcgtccg gtgcaggatt
     2761 tccctgagcg gggtcaccac atggcgcggc tcgagggtgc cggcggcggc ttcgggcaga
     2821 agcggcgtgt agagaaggaa gttgacgtcg ttgaccaggg tcaggcggga tgactgccgg
     2881 ggcagcacct tttcgaggtg gcgggcgacg ttcgcaccgg cgaatccgcc gccgacaatg
     2941 acgatgttcc aggccatgcg gcgagtatag gcagcggacc ctttcgccga atcgcaaccg
     3001 aaggccgttt gatctgttct aatggaccta tgggtctcac aagcagggca aggggaggat
     3061 tggccggggc actcgccctg accgccgccc tcatcgccgt ctccatcgtt tcttcgtcca
     3121 ccgcaagtgc cgccacgatc gtcaacgaag gtgcgccacc gccgaagatc tcgaaggtct
     3181 ttccgaacag atccagcgtg gtttcggaaa ccatcgcgaa cccggcctcg gccgacgact
     3241 actggacgcc cgaacggctc gcttccgccg tgccggccga aatgccgaat ccgtcggatg
     3301 cgctcgacat ccccgatttc gcgtctggag cgtccgcttc cgcagatggc ggcgacttca
     3361 cccccggcaa cgtgaccgcg tttccgcagc gtgtccatgg caagatcttc ttttcggtcg
     3421 gcgtaaacaa cttcagctgc tcaggaaccc tgatcgactc ggccaatcag agtgtcgtct
     3481 tcaccgccgg ccactgtgtc ttcgaccagg agacccatgg ctttgtcagc aatctcattt
     3541 tcctgcccgg gtacgaaaac ggtgttgccc agctcggagc cgcaaacggg gccagcgtct
     3601 tcactacctc gcagtgggcc caaagcgggg ccaatgccta cgacatggga gtggtcgtgc
     3661 tcgaccagcc attcgagaag ttctacggat cccggcagat cgccttcgac ctgaatccga
     3721 agggtctcaa ttacacgatc tatggctacc cgtcgaagcc caacccgccg tacaacggcg
     3781 aagtgcttca gggctgccat tcgggcttcg ccggccgcga cgacggcaac ggcaacatca
     3841 ccccgtaccc ggtcgcggct tcaccctgct tcatgcagca gggggccagc ggcggcgggt
     3901 gggtggtcgg cggcaactac gtgaattcgg tcgtcagcta cggctactgc gattcggtac
     3961 cgagcacctg cgggttcatc ttcgggccgt acttttcgaa tgccgccaag tcgctctacg
     4021 ttcaggccgg cggctcgccg gctcccacga tcaagctgaa gagcggaccg aagggcgtcg
     4081 tgcgcaagcg tacggtgaac ttccagttca ccggctcggc cgcgaccctg ctcgggttca
     4141 ggtgcaggtt tgaccggcag aaggaagttg cctgctcctc gaagatctcg atcaggcggc
     4201 tcagcccggg caagcacata ctcagggtct gggcggtcga tcagaccgga cacaagagca
     4261 aaaagccggc cgtccgcagc ttccgggtcg tgctgccccg ccgctgagaa gcgtgaacta
     4321 aaggcccagg tgctgacgga ggaggcggtt gacctcgcct ccgtcggcgc ggccctgggt
     4381 ggccttcatc acggctccga ccagggcgcc gatcgccttc tcgttgcctt cccggacctg
     4441 ttcggccggt ttcggattgt cctcgatcgc ctgaatgacg atcttctcca gctcgcctga
     4501 atcggcgatc tgttccaggc ccttttcagc caccacggtg accggatcgg ttccggaggc
     4561 gaccatctcc tcaagcaccg tcttgccggc cccgtgggag atcttcttcg cctgaaccag
     4621 gccgatcagc gtagccaggg actccggccc gactttcgag tcggccaggg cctggtcatc
     4681 ctgtttgcgc agctcggcgg caaactcgcc ggtcacccag ttcgcggcaa cccgcggttc
     4741 gatcgattga tcggccgcca gcacgccctc gaagtacccg gcccattcga gctcgaacga
     4801 gagcagcagg gcagcctgtt ccgatacgcc gatcccttcg tagcgggcgg cccgggcggc
     4861 cggcagttcg ggaagcgagc tcgcagcttc ctccagcatc gccggggtcg gcacgaccgg
     4921 gaccagatcg ggttccggca ggtagcggta gtcgtgtgct tcttccttgg aacggagcga
     4981 gtgaagctcc ccggtcgccg gatcgaagtg aagtgtttcc tggaccacct ggccgccgct
     5041 cgcgacgact tccttctggc gggcgacctc ggcctcgatg ccgcgttcga tgaagcggaa
     5101 ggagttcatg ttcttcatct ccgtcttggt accgaactcg gtgctgccga ccgggcggat
     5161 cgagacgttg gcgtcgcagc gcagcgagcc ttcctccatg ttcacgtcgg agacgccgag
     5221 ttgcttgatc gtcgccctca ggagctggcc ccattcccgg acctgggcgc cggaacgaag
     5281 gtccggttcg gtgacgatct ctaccagcgg ggttccgccc cggttgaaat cgacctccga
     5341 ggcgtcggaa cccttgatcc ggccgccgcc tccggtgtgg atcgtcttcg ccgcatcctc
     5401 ctcgaggtgg gcgtgatgga tccgtacttc gccgagccgg ccgttgaccg caaagggcct
     5461 ggctgcctgg gtgatctggt acgccttggg gttgtccgga tagaagtagt tcttccggtc
     5521 aaaggtggag acgggggaaa gttcgcagcc gagagcggcg ccgatctgaa gcgcgtactc
     5581 gatcgcctgg gcgttgggca ccggcagcac gccgggcagg gccaggcaaa cggggcaggt
     5641 gtggacgttc ggatcgtcac cgaaggagag ttcgcagccg cagaacatct tggttctggt
     5701 cgaaagctgg acgtgaatct ccagcccgat caccgcttcg tattcggtgc tcacgagaca
     5761 cccccttcga gggcggcgga accatcgaag ccaatcgcct gttccagcgc gtaggaggcc
     5821 tcgagcagct tctgctcggc gaaggcgggg gcggcgatct ggaagccgac cggcagttcg
     5881 gagcctcccc catccggttc ggccagtccg gccgggatcg agatggccgg caccccggcc
     5941 agcgagatcg gcaccgtgaa cacgtcactc aggtacatcg tccaggggtc ggaggtcttc
     6001 tcgccaagtt tgaaggcaac ggtcggcgag gtcggcgtga ccacgaagtc gacctcaccg
     6061 aaggccttct cgaagtcttc gacgatgcgg gtgcgaaccc gctgggcccg gccgtaatag
     6121 gcgtcgtagt agcccgagga aagggcgtag gtcccgagca tgatgcgccg cttgacctcg
     6181 ggcccgaagc cgtcttcgcg ggtcacctcg tacatcgaga gcaggtcttc gttgctggcc
     6241 ctgaggccgt aacggacccc gtcatagcgt gagaggttgg tcgaggcttc ggccggggcg
     6301 agcacgtaat aggcggagat cgcgtgcccg gcgtgaggca gttcgatgtc gacgatctcg
     6361 ccgccgagtt cggcgatccg gatcagcgtg tcctcgaacc gggccaggac accggcttcg
     6421 attccctcgc ccatcagggc ccggggcacg ccgaaggtca tgccgtcaag ccgttcggcg
     6481 tccggcgcct tgatcccgcc ttcgatgccg accgaggtcg aatccatttc gtcacgcccc
     6541 tgaagcacgt tcaggagcag ggccgcgtcg gtgacgttcc tggtcagcgg cccgcattga
     6601 tcgagagaag aggcgaaggc gatcatcccg aagcgggaaa tcgcgccgta ggtgggctta
     6661 aggccgacga tcccgcagaa tccggccggc tggcgaatcg aacctccggt gtcggtgccg
     6721 agcgaccagg gggcgagtcc gccggcgacg accgccgccg acccgcccga agatcctccc
     6781 ggcacccttt cggtgtccca ggggttgagg caggcaccga aggccgaatt ctcgttcgag
     6841 gagcccatcg cgaactcgtc catgttggtc ttgccgagca tcggcatccc cgccgccatg
     6901 gccttcctca ccgaggtggc cgtgtatggc ggcacgtagc cgccgagaat cctcgaggcg
     6961 gccgtggtcg gcacgccttc ggtgcaaaag atgtccttga ttgcgagcgg caccccgtcg
     7021 agaggacccc cctcgcccag tccctggcgg gcagaatcac gatcggcctg ccagaggtat
     7081 ccgttcagcc ggtcgcgacc ggcccggtcg agccagaagg cgagtacttc gtcggccgaa
     7141 agctcgccgc ttttgatctt tgccgccgtt tcagcaccgc tggcggccag c
//
LOCUS       DAOQKN010000133         7151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_5180_length_7151_cov_5.530017, whole genome shotgun sequence.
ACCESSION   DAOQKN010000133 DAOQKN010000000
VERSION     DAOQKN010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 7151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7151
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5180_length_7151_cov_5.530017"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="PLJ64_09000"
     CDS             complement(<1..749)
                     /locus_tag="PLJ64_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNC93658.1"
                     /translation="MNKYGPLGRLGRWTTGNFRVVAAVWLVIAIGLGFFAPKAEHALS
                     GAGWEATGSESVDARKIVDQEFGGMSSSALMVVVHSDDSAFGTAAFDRTLNQAASVLR
                     ESDDVKAVVMPSRKSWVSADGHTAVIQAGAAADSNRMVRAADDIKVDLAALDQAGTTV
                     SLTGSSGMWSDFNQANKEAMMKSELISWPVTLLILVLAFGSLVAAGLPLMLTIIGLMA
                     AAGSLYLGTQLFDISIWAMNFAMMFALALGI"
     gene            complement(746..1015)
                     /locus_tag="PLJ64_09005"
     CDS             complement(746..1015)
                     /locus_tag="PLJ64_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNC93659.1"
                     /translation="MYFRSETMMEASCETKGRRGWYLERVLFAMAGTVSLAGGLLALL
                     VSPWFALLPVLAGINQWLYVTVGACPASLVLSRVTSLRSAAETGR"
     gene            complement(1096..1431)
                     /locus_tag="PLJ64_09010"
     CDS             complement(1096..1431)
                     /locus_tag="PLJ64_09010"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNC93660.1"
                     /translation="MALPTPLPPELVELITARFKLLSEPMRIRILDQLREGEQSVGGL
                     AEILDTSQQNVSKHLQALYAGGAVARRKEGNTVYYGIADESLMSMCEAVCEGVEQRIA
                     ELSQIVTQA"
     gene            complement(1485..2198)
                     /locus_tag="PLJ64_09015"
     CDS             complement(1485..2198)
                     /locus_tag="PLJ64_09015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNC93661.1"
                     /translation="MSTTPQLPTTLDQEIIRCFEPEIQAEMTDPERLAKIQQEFEEGF
                     EVMKPVGPAVCVFGSARTRPGHPEYEQARRVGAGIARQGYAVITGGGPGSMEAANRGA
                     KDEEGLSVGLNIELPHEQGANPFLDIGKEFHYFFVRKLMFVRYSWSFVIFPGGFGTLD
                     EMFEALTLIQTLKARPHPVVLAGADFWQDLIDWIARRLAGEGRISPSDMDLFTVCDED
                     DEIISSAIHGLPNLPYGLA"
     gene            complement(2209..3681)
                     /locus_tag="PLJ64_09020"
     CDS             complement(2209..3681)
                     /locus_tag="PLJ64_09020"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF015406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNC93662.1"
                     /translation="MSENRLTELMWFRRDLRTTDLPALAEAARADRILPCFIFDDRLL
                     TGGRFTSPRRTSFMLGCLKSLRQDLRDLGADLILRRGRPEEEIPWLATEAGATTVHWT
                     EDFSPFATGRDRRVGRALAGAGITAIPHPGACIADRPQEIQTGQGRPYTVFSPFNRAW
                     QEAPRRPAEARPESLKLAKRSGGGQFEAGELPDLAELGLAADDSDWQEAFEPGEAAGR
                     VAAKEFLNRGLSEYDERRDNPAGGSSRLSPWIRWGCLSPLELEAKLGRRQGEGPRSFR
                     SELAWRDFYASVIASHPEVTRLEYQERYRGTLDWQSDEGLLAAWREGRTGYPLVDAGM
                     RQLVAEGWMHNRVRMVVASFLTKDLHLDWREGERHFMELLIDGDVASNNGGWQWVAST
                     GTDPAPYFQRMFNPMTQQEKFDPEGEYVRRWVPELRKVPQGHLVRPWKMTGEEQEEAG
                     CLIGKDYPEPVVDHAEERRFAIERYRAAADADPSDGDWRG"
     gene            complement(3686..5203)
                     /locus_tag="PLJ64_09025"
     CDS             complement(3686..5203)
                     /locus_tag="PLJ64_09025"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNC93663.1"
                     /translation="MGKGSFMLVLLVGLTIAFGSATSAASLPRLKADPDTARGGRIVD
                     TKGREVILRGVNVNSLGQYWQGTSKPPTLPLEKKDPARIAAIGWNLVRLIVSWSRVEP
                     RPGVINTAYLDRVARWTGLMEEEGIYVMLDFHQDAWGPTLAAPDGQVCTPPAQPAFGW
                     DGAPGWATLDDGQPRCFTSNREINPAVRAAWTNFFDDKPASDGVGIQTHYVRMLKRVA
                     KRFAKDPGVAGIDIMNEPNAFGPVQTEQLGRFYGRSLKSIRAGEKAGGGFRHLVFFEP
                     GVLWSLLGSGAPGPFNFDSNVVYSPHLYGGSIGATGPPSRESFENARAEAAGFGGAPV
                     LTGEWGGDPLRADGTGEDYFSQHLDLQDEFRIGSVLWTWKQSCGDPHAATHDPDTAPP
                     LPPWSVYRMDCEGNSNRIAGQYGKLVRSLRRAYVRRAPGRLASMSFDPDTGRLRASGK
                     GARRTDGPLEVYLPLTRARLTLKGLNRVHAKKVGPGMVVWLAPKGGPWELRAKVG"
     gene            5303..5740
                     /locus_tag="PLJ64_09030"
     CDS             5303..5740
                     /locus_tag="PLJ64_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93664.1"
                     /translation="MDRKVGAALAVAIIGVIVGVVGIVMAKDAKDANQDTQAQLEQQI
                     KQSNKSSAAKVAAAAAAAKVIENKAASKAAESEKQDESSLKSAESKDSSQASANASDI
                     QTLEDKVDELSTEIGNVSGQLKSNTAKVNDRIDELAARVNAGG"
     gene            complement(5809..7065)
                     /locus_tag="PLJ64_09035"
     CDS             complement(5809..7065)
                     /locus_tag="PLJ64_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93665.1"
                     /translation="MTHRIPHPKRILLFLTVAIAALIGFAGTASAAGQGSIVFIKNND
                     IWLANPDGSHQFRVTWDGSEDFPYLSPSQAADGTIAAGHVRDIVLMRQNGDVIREIEP
                     PALINSVSHPMDGVPVDVAISPNGKRIAWSFVGYECDGTVNCGPRTATGYTDSSGRTP
                     VSKYGSTYFHNPSWVGNSRTLQGGGFGSQVMIHDLGQDPVHWFDDSDYAEYSTDLGDS
                     ELSPDGSRLASIRGYGSETQLYTFEVSGNATSGPAPPVPSPDCMTGTEEGIAGPTWAP
                     DSRHLAWQNDEGVWTGDLSDCGNAGLELVIPGASEPDWGPAAVNPSPGGVRLPFASLG
                     RALKKGLTIDFTSIGKGRVDFTVKLGRTVVAADHGTAPNAGPLRAVVRFKASARKRLS
                     RMKTVRLSISVSQSGKTATAAGTLKK"
BASE COUNT         1215 a         2351 c         2385 g         1200 t
ORIGIN      
        1 tcgatgccga gcgcgagggc gaacatcatc gcgaagttca tcgcccagat cgagatgtcg
       61 aagagttggg tgcccaggta gagcgaaccc gccgcggcca tcaggccgat gatggtcagc
      121 atcagcggca gtccggcggc aaccagtgaa ccgaaggcga ggaccaggat cagcagcgtc
      181 accggccagg agatcaactc cgacttcatc atcgcttcct tgttggcctg gttgaagtcg
      241 gaccacatcc cggaagagcc ggtcagggaa accgtggtcc ccgcctggtc gagggctgcc
      301 aggtcaacct tgatgtcatc ggcggcgcga accatccggt tcgagtcggc ggccgcgccg
      361 gcctggatca ccgcggtgtg gccgtcggcc gagacccagc tcttcctcga gggcatgacc
      421 acggccttga cgtcatccga ctccctgagc acggaggcgg cctgattcag ggtccggtcg
      481 aaggcggcgg ttccgaaggc cgaatcgtcg ctgtgaacga cgaccatcag ggccgaggag
      541 gacatgccgc cgaactcctg atcgacgatc ttgcgggcat cgaccgattc cgagccggtc
      601 gcttcccagc cggctccgga aagcgcgtgc tcggccttcg gtgcgaagaa gcccagcccg
      661 atcgcgatga ccagccagac cgccgcgact acacggaagt tcccggtggt ccaccggccg
      721 agcctcccga gtggtccgta cttgttcatc ggccggtttc cgcggcggac cgcagcgaag
      781 tgacgcggct gaggaccagt gacgccggac aggcgccgac cgtgacgtaa agccactggt
      841 tgatcccggc caggaccggc aggagggcga accaggggga gaccagcagg gccaggaggc
      901 ccccggccag gcttaccgtt ccggccatcg cgaagagcac ccgttcgagg taccagccac
      961 ggcggccctt ggtttcacag ctcgcttcca tcatcgtctc cgatctaaaa tacaagttca
     1021 caactgtata gtggaataga ttagcagaga ttcggggcgc cggcttgagc ggcgtgaacg
     1081 ggaggttccg gaagatcagg cctgcgtgac gatctggctg agctcggcga tgcgctgttc
     1141 gacgccctcg cagacggctt cgcacatcga catcaggctt tcgtcggcaa tcccgtaata
     1201 gacggtgttg ccttccttgc ggcgcgccac cgcgcctccg gcgtagagcg cctggaggtg
     1261 cttggagaca ttctgctggc tggtgtcgag gatctcggcc aggccaccga cgctctgctc
     1321 tccttcgcga agctgatcga ggatccggat ccgcatcggt tccgagagca gcttgaagcg
     1381 ggcggtgatc agttcaacca gctcgggcgg gaggggagtc ggcagggcca tgccttcagc
     1441 ttacagccgg acaactgttt gctcgccgag ttgcccggcg gcgttcaggc gagtccgtaa
     1501 ggcaggttcg gaaggccgtg aatcgcgctg ctgatgatct cgtcatcttc gtcgcagacc
     1561 gtgaagaggt ccatgtcgga aggggagatc cggccctcgc ccgccaggcg cctggcaatc
     1621 cagtcgatca ggtcctgcca gaagtcggcc ccggcgagaa cgacgggatg cggccgcgcc
     1681 ttcaatgtct ggatcagggt cagcgcctcg aacatctcgt ccagcgtgcc gaagccgccg
     1741 ggaaagatca cgaacgacca cgagtagcgc acgaacatca gcttgcggac gaagaagtag
     1801 tggaactcct tgccgatgtc gagaaagggg ttcgctccct gctcatgggg cagttcgatg
     1861 ttcaacccga ccgagagtcc ttcctcgtcc ttggcgccgc ggttcgccgc ctccatcgaa
     1921 cccggaccgc cgccggtgat caccgcatag ccctggcggg cgatgccggc cccgacccga
     1981 cgggcctgtt cgtactcggg gtggccgggc ctggtcctcg ccgacccgaa cacgcagacc
     2041 gccggcccga ccggcttcat cacctcgaat ccctcctcga attcctgctg gatcttcgcg
     2101 aggcgttcgg gatccgtcat ctcggcctga atctcgggct cgaaacagcg gatgatctcc
     2161 tggtcgagag tggtgggcag ctgtggagtc gtactcatcg gcggcagcct atccccgcca
     2221 gtccccgtcc gacgggtctg cgtcggccgc ggcacggtac cgctcgatcg cgaatcgccg
     2281 ctcctccgcg tgatcgacta ccggctccgg atagtccttg ccgatcaggc aaccggcctc
     2341 ttcctgctct tcaccggtca tcttccaggg gcgaaccagg tgtccttggg gaactttccg
     2401 caactccggc acccaacgcc gcacatattc cccttcgggg tcgaactttt cctgctgggt
     2461 catcggattg aacatgcgct ggaaataggg ggcggggtcg gtcccggtcg aagcgaccca
     2521 ctgccagccg ccgttgttcg aggcgacgtc gccgtcgatc aggagttcca tgaaatgcct
     2581 ttcgccctct cgccagtcga ggtggagatc cttggtcaaa aaggaagcga cgaccatccg
     2641 cacccggttg tgcatccagc cttcggccac cagctggcgc atgccggcgt cgaccagggg
     2701 gtagccggta cggccctccc gccaggctgc gagcaacccc tcgtcgcttt gccagtcgag
     2761 cgtgccccgg tatcgctcct ggtactcgag cctggtcacc tccggatggc tggcgatcac
     2821 cgaggcgtag aaatcccgcc aggcaagctc ggaacggaag ctgcgcggac cttcgccttg
     2881 ccgccgaccc agtttggctt cgagctccag gggcgaaagg cagccccagc ggatccaggg
     2941 agaaagccgg gaggatcccc cggccggatt gtcacgccgc tcgtcatatt cggaaagtcc
     3001 ccggttgaga aactcttttg cggctacccg gccggccgct tctcccggct cgaaggcttc
     3061 ctgccagtcg gaatcatctg ccgcaagtcc cagctcggct aggtcgggca gttcaccggc
     3121 ttcaaactgg ccacccccgg acctcttcgc cagcttcagg ctctccggcc gcgcttcggc
     3181 cgggcgccgc ggcgcttcct gccaggcgcg gttgaaaggg ctgaacaccg tgtaggggcg
     3241 gccctgcccg gtctggattt cctgtgggcg gtcggcgatg caggcaccgg ggtgagggat
     3301 cgcggtgatc ccggccccgg ccagtgcgcg gccgaccctc cggtccctcc cggtcgcgaa
     3361 gggggaaaag tcctcagtcc agtggacggt cgtcgccccg gcttcggtgg cgagccacgg
     3421 aatctcctct tccggcctgc cccgccggag aatcaggtcc gcccccagat cgcgcagatc
     3481 ctgccgcagg ctcttcaggc agcccagcat gaagctggtt cgccggggcg aggtgaacct
     3541 gccaccggtg agcaaccggt catcgaagat gaagcagggc aggatccggt cggcgcgggc
     3601 cgcctcggcc agggcgggaa ggtcggtggt tcgcagatcc ctgcgaaacc acatcaactc
     3661 ggtgagcctg ttctcgctca aaccttcagc cgactttggc ccggagctcc cacgggcccc
     3721 ccttgggcgc gagccagacg accatgccgg ggccgacctt cttcgcgtga acccggttca
     3781 gtcccttcag ggtcaggcgt gcccgggtca ggggcaggta aacctccaga ggaccgtcgg
     3841 tgcggcgggc gcccttcccg ctcgccctga gacggccggt gtcgggatca aagctcatcg
     3901 aggcgagccg gcccggtgcc cggcgaacgt aggcccggcg cagtgacctg accagctttc
     3961 cgtactgccc cgcgattcga tttgaattcc cttcgcagtc catccggtaa accgaccagg
     4021 gtggcagcgg cggcgccgtg tcggggtcat gggtggcggc gtgggggtcg ccacaggact
     4081 gtttccaggt ccagagcacc gaaccgatcc ggaactcgtc ctgaaggtcg aggtgctggc
     4141 tgaagtagtc ctccccggtg ccatcggccc ggagcggatc gccaccccat tcaccggtca
     4201 gtaccggggc gccgccgaag cccgccgcct cggcccgggc attctcaaat gactcgcggc
     4261 tcggcggacc ggtcgcgccg atgctccccc cgtagagatg gggcgaatag acgacgttcg
     4321 agtcgaagtt aaaggggccg ggagcgccgc tgccgagcag cgaccagagc acccccggtt
     4381 cgaagaagac gagatgccgg aagccgccgc ccgccttctc gccggcccgg atcgacttca
     4441 gcgaacgacc gtagaaccgg ccgagctgtt cggtctggac cgggccgaag gcgttgggct
     4501 cgttcatgat gtcgatcccg gcgacgccag ggtccttcgc gaaccgcttc gctacccgct
     4561 ttagcatccg cacgtagtgg gtctggatcc cgaccccgtc cgaggccggc ttgtcatcga
     4621 agaaattggt ccaggcggcg cgcacggccg gattgatctc gcggttgctg gtgaagcagc
     4681 ggggctggcc gtcatcgaga gtcgcccagc ccggggcgcc gtcccagccg aaggcaggct
     4741 gagccggggg agtgcagacc tggccatcgg gggccgccag ggtcggcccc caggcatcct
     4801 gatgaaaatc gagcatcacg tagatgccct cctcttccat caggccggtc cagcgcgcga
     4861 cccggtccag gtaggcggtg ttgatcaccc ccggcctcgg ttcgacccgc gaccaggaga
     4921 cgatcaaccg gaccaggttc catccgatcg cggcgatccg ggcggggtcc ttcttctcga
     4981 gcggcagggt gggcggcttc gaggtgccct gccagtactg gccgagggaa ttcacattga
     5041 ccccgcggag gatcacctcc cggcccttgg tgtcaacgat ccggccaccg cgcgcggtgt
     5101 cgggatcggc cttgagccgc ggcaggctgg ccgccgaggt tgcggatccg aacgcgatcg
     5161 tcaggccgac cagcagcacc agcatgaaac tccccttgcc catcggccca gtgtagtcca
     5221 gccgtcaggt ccggctgtca cagtcccggc agccagtcca aactcgcatt ccggcggtca
     5281 gtcctccggg gaaggaaacg aaatggatcg caaagtcgga gcagccctgg ccgtcgcaat
     5341 catcggcgtg atcgtcggtg tggtcgggat cgtcatggcc aaggacgcca aggacgcaaa
     5401 ccaggacacc caggctcagc tcgaacagca gatcaagcag agcaacaaga gctcggcggc
     5461 caaggtcgcc gcggccgcag cggcagcgaa ggtgatcgag aacaaggccg ccagcaaggc
     5521 agccgagtcc gagaagcagg acgagagttc gctgaagtca gccgagtcga aggattcgtc
     5581 ccaggcgtca gccaacgcct cggacattca gaccctcgaa gacaaggttg acgagctgag
     5641 taccgagatc ggcaacgtct cggggcagct caagagcaac acggcgaagg tcaacgaccg
     5701 gatcgacgag cttgcggcga gggtgaacgc cggcggctga tgagacgggt cgcggcttca
     5761 tggccgcaac ccgccggttc gtctctcaag ccgcttcagc ctggcccgtt acttcttcag
     5821 cgtcccggcg gccgtcgcgg tcttgccgga ctgactgacg ctgatcgaca gcctgaccgt
     5881 cttcatccgg ctcaggcgct ttcgcgctga tgctttgaac ctgaccacgg cgcggagcgg
     5941 tccggcattg ggagcggtgc cgtgatcggc ggcgaccacg gtccggccga gtttcaccgt
     6001 gaagtcgacc cggcccttgc cgatcgaagt gaagtcgatc gtcagcccct tcttcagcgc
     6061 ccggccgagg ctggcgaagg gcaaccggac cccgcccggt gacgggttga ccgcggccgg
     6121 gccccagtcg ggctcggagg cgcccgggat taccagttcc aggccggcgt tcccgcaatc
     6181 gctgaggtca ccggtccaga cgccttcgtc gttttgccag gccaggtgcc tgctgtccgg
     6241 ggcccaggtc ggcccggcga tcccttcctc cgtgccggtc atgcagtccg gcgagggaac
     6301 cggcggggcc ggtcccgagg tcgcgttgcc gctcacctcg aaggtgtaga gctgggtctc
     6361 ggatccgtag ccgcggatgc tggccagtcg ggagccgtcg ggagagagtt cgctgtcacc
     6421 gagatcggtc gagtactcgg cgtagtcgct gtcgtcaaac cagtggaccg ggtcctgtcc
     6481 gaggtcgtgg atcatcacct gggagccgaa gcctccgccc tgaagggtgc gggaattgcc
     6541 gacccaggag gggttgtgga agtaggtcga gccgtatttc gagaccgggg tccggccgct
     6601 cgagtcggtg tagccggttg cggtgcgcgg cccgcagttg accgtcccgt cgcattcgta
     6661 gccgacgaag gaccaggcga tccgcttgcc gttcggcgag atcgccacgt caaccggaac
     6721 gccgtccatc ggatggctga ccgagttgat cagggccggg ggttcgatct cccggatcac
     6781 gtctccgttt tgccgcatca gcacgatgtc gcgcacgtgc ccggccgcga tcgtcccgtc
     6841 ggcggcctgg ctgggggaga ggtaggggaa gtcctcgctt ccgtcccagg tgacccggaa
     6901 ctgatggctc ccgtcgggat tggcgagcca gatgtcgttg ttcttgatga agacgatcga
     6961 tccctgcccg gccgccgagg cggttccggc aaagccgatc agggccgcga tcgcgacggt
     7021 caggaacaga agaattcgct tgggatgggg gatgcggtgg gtcattggtc tcctcgtcgg
     7081 gtgctgtgga aagcaaccta gaaaggggac cttgcccgga tcttgcggac tgattgcgcc
     7141 gggccgggag g
//
LOCUS       DAOQKN010000134         7114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_5214_length_7114_cov_7.023091, whole genome shotgun sequence.
ACCESSION   DAOQKN010000134 DAOQKN010000000
VERSION     DAOQKN010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 7114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7114
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5214_length_7114_cov_7.023091"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="PLJ64_09040"
     CDS             complement(<1..512)
                     /locus_tag="PLJ64_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93666.1"
                     /translation="MQARLEEALRIALRTDRPVELTVAGRTDAGVHALGQVASFSFDG
                     EMPPAIVRSLNGLTPRGIAVRAVTPVSGFDARKDAVSRTYCYRVLTRRPDSPFAVNRA
                     WWVSRPIDRDALDSCAGALIGRHDFTAFTPTETYHKRFERIIHSAAWTDENGLVDPAT
                     GFSAGGDTIQ"
     gene            complement(587..2056)
                     /locus_tag="PLJ64_09045"
     CDS             complement(587..2056)
                     /locus_tag="PLJ64_09045"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93667.1"
                     /translation="MVAVFAAGLVMLAAATPARAAWGTPILGNAPAGVATDSSGNLYV
                     AEAYGNRFVKYSATGTQLMAVGTTGSGQAQFNSPNGIAVESGGAIFVADTGNNRIQRF
                     YPNGSYQTEWGTFGTALGQFKQPAAVAVDGGGNVYIADTDNNRVVKCNAWGWCGSAFE
                     FTAIGGIASPLGVATDGSGNVYVSDSGNDRIIKSDSSGNVLSVWSTFGSGQGQVNNPA
                     GLAIGAGNTLFVADKGNSRVQRLSTGGAFLSEYIVPSPYVASQPTSVAIGPGTTINVA
                     NPWNNRVDRWDPPTGPTGPTGPTGPTQPTGPTGPTGPTQPTGPTGPTNPTGPTGPTGS
                     TDGPVINPPNGNNGGNNGNNGNNGNNGNNGNNGNNGNNGNNGKGKTKQAVIKPVKVWK
                     EGGGSVVHPGEAVNMKVSIKNSGDLRATNVYTYMDKKALSGRPSVDVKRGFNTRVITP
                     GWTVTGTVTIFFGRRAHGKVAITAQAWGNYGKKILYIRR"
     gene            complement(2299..2652)
                     /gene="rplQ"
                     /locus_tag="PLJ64_09050"
     CDS             complement(2299..2652)
                     /gene="rplQ"
                     /locus_tag="PLJ64_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNC93668.1"
                     /translation="MRHAKKRNKLGRDSAHRKALLSNLSKEIIEHERIKTSQAKAKAA
                     KPEIEKLITLGKRGDLHARRQALSVLRQDKFAVHKLFEEIAPRYAERNGGYTRIVKIG
                     PRKSDSTEMVFLELV"
     gene            complement(2682..3650)
                     /locus_tag="PLJ64_09055"
     CDS             complement(2682..3650)
                     /locus_tag="PLJ64_09055"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNC93669.1"
                     /translation="MSTDFQVPTISSEQVEENKGVFTIEPLDKGFGYTFGSSLRRVLL
                     SSLGGAAITSVRIEGVSHEFSNIAGVKEDVTDIVLNLKDLVVRMHTDADAVEAPIVAT
                     GPGNVTAADIDLPAGVEILNPDAPIATLEKKTRLEMYVTIGRGRGYRPAEENKDADQP
                     IGVIPIDSIFSPIRRASYTVEAARVGQRTDFDKLTLELETDGSIEPNTALREASEILI
                     KSLAIFSDPDRVEELTAREPVVAEGIVADNGAPVATGGDNDILIDELELSVRSYNCLK
                     RAGIETIGQLLAKSESELAAIPNFGSKSIEEVIENLEGRGHHLRQD"
     gene            complement(3700..4092)
                     /gene="rpsK"
                     /locus_tag="PLJ64_09060"
     CDS             complement(3700..4092)
                     /gene="rpsK"
                     /locus_tag="PLJ64_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018224911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNC93670.1"
                     /translation="MAAKKPAKSRRRVRKNIAVGQAHIKTSFNNTIVTLTDPEGNVIA
                     WESAGSAGFKGSRKSTPFAAQVTADAAAKKGMEHGLQKVEVFAKGAGSGKDTAVRSLQ
                     AAGLEVTSVKDVTPQAHNGCRPRKRRRV"
     gene            complement(4110..4487)
                     /gene="rpsM"
                     /locus_tag="PLJ64_09065"
     CDS             complement(4110..4487)
                     /gene="rpsM"
                     /locus_tag="PLJ64_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNC93671.1"
                     /translation="MARIAGVNIPLNKRAEIGLTYVYGIGQSTAQEILDQVGIDRNTQ
                     VKDLTEDEVIKLRDAVEQHEVEGDLRRERSQNVKRLMEIGSYRGLRHRRGLPVRGQRT
                     KTNARGRKGPRRMSVAGKKKPAK"
     gene            complement(4491..4604)
                     /gene="rpmJ"
                     /locus_tag="PLJ64_09070"
     CDS             complement(4491..4604)
                     /gene="rpmJ"
                     /locus_tag="PLJ64_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HNC93672.1"
                     /translation="MKVRASVKPMCEKCKIIRRRGRVLVICENPRHKQRQG"
     assembly_gap    4859..4958
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4959..5705)
                     /gene="map"
                     /locus_tag="PLJ64_09075"
     CDS             complement(<4959..5705)
                     /gene="map"
                     /locus_tag="PLJ64_09075"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNC93673.1"
                     /translation="MIIRKTDDQIEKMAAAGAVHARCMKMLTAKVRPGVTTAELDEVA
                     EKFIRSQGGVPTFKGFRGFPGSICASPNSMVVHGIPGPYKLQKGDILSIDIGVTLDGW
                     VADGASTIPVGPIDETTQKLLTVTKESLDLAAAQMVPGNRVGDIGHAVQSHVEANGFS
                     IIRSLVGHGVGQNMHEEPQIANYGTPGRGPEIEEGMVFAIEPMVNVGGPDVYMDDDGW
                     SVFSDDGSMAAHFEYTVAATKDGPRILTPWD"
     gene            complement(5716..6390)
                     /locus_tag="PLJ64_09080"
     CDS             complement(5716..6390)
                     /locus_tag="PLJ64_09080"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNC93674.1"
                     /translation="MAELNLILLGPPGSGKGTQGERLQEDLEYPYYATGDILRAAVKE
                     GTEVGNAAKEYMDRGDLVPDEVIIGIIAERLESGEADHGFILDGFPRTVVQAEALDAE
                     LHKLGRQLTAVILIDVEDDEIVNRLSGRRVCQEKGHVYHVDNSPPEQEGVCDVDGSPL
                     YIRDDDKPEVVRHRLEQYQEKTAPLVDYYKKQGLLNKVEGAEDPDRVSEKIRTLVSTL
                     SLEEGS"
     gene            complement(6401..>7114)
                     /locus_tag="PLJ64_09085"
     CDS             complement(6401..>7114)
                     /locus_tag="PLJ64_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY family transport protein"
                     /protein_id="HNC93675.1"
                     /translation="LLIFASIVSGVLPGIQTWWNNPDQVFVVLLPFICLAVIVAIVFV
                     QEGQRRIPVQYAKRVVGRKMTSGGSTYLPLRVNMAGVIPVIFAASIMALPPTIGQLIN
                     TPAALDFAAFLSPSSWAYVVGEVFFIIIFTYFYTAVTFNPVDQADNLKKYGGFIPGVR
                     PGRPTAEYLDRVLSRLTFPGALYLGAVAAFPTILFNNVGGGFFFGGTSILIVVGVALD
                     TVKQLEAQLMMRNYEGFLR"
BASE COUNT         1129 a         2141 c         2393 g         1351 t
ORIGIN      
        1 aactggattg tgtctccgcc cgcggagaaa ccggtggccg ggtcaaccag tccgttctcg
       61 tccgtccatg ctgcggaatg gatgatccgc tcgaaccgct tgtggtaggt ctcggtcgga
      121 gtgaaggcgg tgaagtcgtg ccggccgatc agcgccccgg cgcaggagtc gagcgcgtca
      181 cggtcgatcg gccgtgagac ccaccaggcc cggttcacgg cgaacgggct gtcgggacgc
      241 cgggtcagga cgcggtaaca ataggtccgc gagacggcgt ccttgcgcgc atcgaagccg
      301 gagaccgggg tcaccgcccg gaccgcgatc ccccgcgggg tgagtccgtt caggctgcgc
      361 acgatcgccg gcggcatctc gccgtcaaag ctgaaactgg cgacctgacc gagggcgtgg
      421 accccggcgt cggttcgtcc ggcgacggtc agctcaaccg gccggtcggt tcgcagggct
      481 atccgcagcg cttcttcaag ccgcgcctgc acggtgtcga cgccaccatc ctgcttcgcc
      541 cagccccgga aggcggagcc gtcgtactcg atgtcgagac gccagttcag cggcggatgt
      601 agaggatctt cttgccgtag ttgccccagg cctgggcggt gatcgccacc ttgccgtgag
      661 cgcgcctgcc gaagaagatc gtgacggttc cggtgacggt ccagcccgga gtgatgaccc
      721 tggtgttgaa acctcgcttg acgtcgacgc tgggccggcc ggagagggcc ttcttgtcca
      781 tgtaggtgta gacgttggtg gccctcaggt caccgctgtt cttgatcgaa accttcatgt
      841 tgacggcctc gccgggatgg acgacgctgc cgccgccttc cttccagacc ttgaccggct
      901 tgatcacggc ctgcttggtc ttgcccttgc cgttgttgcc gttattgccg ttgttcccgt
      961 tgttgccgtt attgccgttg ttcccgttat taccgttgtt gccgttattg ccaccgttgt
     1021 tgccgttcgg cgggttgatc acgggaccat cggtggaacc ggtcgggccg gtcggcccgg
     1081 tcgggttggt gggtccggtc gggccggtcg gctgggtcgg cccggtcggg ccagtggggc
     1141 cggtcggctg ggtcggcccg gtcgggcccg tggggccggt cggtccggtc ggggggtccc
     1201 agcgatcgac ccggttgttc caggggtttg ccacgttgat cgtcgtgccc ggaccgatcg
     1261 cgaccgaggt cggctgcgaa gccacgtagg gcgaagggac gatgtactcg ctgaggaagg
     1321 caccaccggt cgagagccgc tgaacccggg agttgccctt gtcggcgacg aaaagcgtgt
     1381 tgccggcccc gatcgccagg ccggccgggt tgttgacctg gccctggccg gagccgaagg
     1441 tgctccagac cgagaggacg ttgccgctcg aatccgactt gatgatccgg tcattcccgg
     1501 aatccgagac atagacgttg cccgacccgt cggtggccac tccgagcggg ctggcgatac
     1561 cgccgatggc ggtgaattca aaggccgaac cgcaccagcc ccaggcgttg cacttgacca
     1621 cgcggttgtt gtcggtgtcg gcgatgtaga cgttgccgcc accatcgacc gccaccgcgg
     1681 caggctgctt gaactggccg agcgctgtgc cgaaggtgcc ccattcggtc tggtaactgc
     1741 cgttcgggta gaagcgctgg atccggttgt tgccggtgtc ggcgacgaag atcgccccgc
     1801 cggactcgac cgcgatgccg ttcggcgagt tgaactgggc ctggccggaa ccggtggttc
     1861 cgaccgccat cagctgggtg ccggtcgcgc tgtacttgac gaagcggttg ccgtaggcct
     1921 cggccacgta aaggttgccg gaactgtcgg tcgccacacc cgccggagcg ttgccgagaa
     1981 tcggcgttcc ccaggccgcc cgggccggag tggcagcagc gagcatgacc agtcctgccg
     2041 cgaaaacggc gaccatcgcg gcgaaaattg ccgcccggtt tgtgtgatgc cccctgttgg
     2101 aaaacaactg cctgatccag ttcctttgaa ttcctaagta gcccagtata ggcgcagtat
     2161 cagaaacacc aaataaccgg aaaagaagcg ctttcaaacc ggttccaccg gcccgatgga
     2221 ggttggagca agcgaaaggg gagccgatgg ctcccctctt gcggcgactt gcctgttcag
     2281 aacccgtctg accggagatc agacaagttc gaggaagacc atttcggtcg aatcggactt
     2341 gcggggaccg atcttgacga tccgggtgta gccgccgttc cgctcggcgt agcggggggc
     2401 gatctcctcg aacagcttgt ggacggcgaa cttgtcctgg cgcaggaccg aaagggcctg
     2461 gcgccgggcg tgaaggtcac cgcgcttgcc gagggtgatc agcttctcga tctccggctt
     2521 ggcagccttg gccttggcct ggctggtctt gatccgctcg tgctcgatga tctccttgga
     2581 gagattgctg agcagcgcct tccggtgggc ggaatcccgt cccagcttgt tccttttctt
     2641 tgcgtggcgc atcgtctctc cttaagggtt actcggtaat gctagtcctg gcgcaggtga
     2701 tgcccgcggc cttcgaggtt ctcgatgacc tcttcaatcg acttcgaacc gaagttcggg
     2761 atcgcggcaa gctcggattc ggacttggcc agcagctggc cgatcgtctc gatgccggcc
     2821 cgcttgaggc agttgtaaga acggacgctg agctcgagtt cgtcgatcag gatgtcgttg
     2881 tcgccgccgg tggcaaccgg ggcgccgttg tcggcgacga ttccttcggc aacgaccggc
     2941 tcgcgggcag tcagctcttc gacccggtcc gggtcactga agatcgcgag gctcttgatc
     3001 aggatctcgg aagcttcgcg gagcgccgtg ttcggttcga tcgaaccgtc ggtctccagc
     3061 tcgagggtga gcttgtcgaa gtcggtgcgc tgaccgacac gggcagcctc gacggtgtag
     3121 gaggcgcggc ggatcggcga gaagatcgag tcgatcggga tcacgccgat cggctgatcg
     3181 gcatccttgt tctcctcggc cgggcggtaa ccgcggccgc ggccgatggt cacgtacatc
     3241 tcgaggcggg tcttcttctc gagcgtggcg atcggagcgt cagggttgag gatctcgacg
     3301 ccggccggca ggtcgatgtc ggcggcggtg acgttgcccg gacccgttgc cacaattggc
     3361 gcctcgacgg cgtcggcgtc ggtgtgcatc cggaccacga ggtccttgag gttcaggacg
     3421 atgtcggtga cgtcttcctt cacgccggcg atgttcgaaa actcatgcga aacgccctcg
     3481 atccggacgc tggtgatcgc ggctccgccc agcgaagaga gcagcacgcg acgcaggctc
     3541 gaaccgaagg tgtagccgaa gcccttgtcg aggggctcga tggtgaaaac acccttgttc
     3601 tcttcaacct gctcggacga gatggtcggt acctggaaat cagtgctcat tggatccttt
     3661 tgttgcgggg gaggggaagg cttgcggggc gcccggaaat cagacgcgac ggcgcttgcg
     3721 ggggcggcag ccgttgtggg cctggggcgt cacgtccttg acgctggtca cctcaagacc
     3781 ggcggcctgc agcgagcgga ccgcggtgtc cttgccggaa ccggcgccct tggcgaagac
     3841 ttcgaccttc tgaaggccgt gctccatgcc cttcttggcg gcggcgtcgg cggtcacctg
     3901 ggcggcgaag ggggtcgact tgcgcgaacc cttgaagccg gcgctaccgg ccgattccca
     3961 ggcgatgacg ttgccctcgg gatcggtcag ggtgacgatc gtgttgttga aggaggtctt
     4021 gatgtgcgcc tggccgaccg caatgttctt gcggacgcga cgacgggact tggccggttt
     4081 cttggctgcc atgaaagttc tccagctcct tacttagcgg gcttcttctt gcccgcaacg
     4141 ctcatgcggc gagggccctt gcggccccgg gcattggtct tggtgcgctg gccccgaacc
     4201 ggcaggccgc ggcggtggcg caggccccgg taggagccga tctccatcag gcgcttgacg
     4261 ttctgcgagc gctcgcggcg aaggtcacct tcgacctcgt gctgctcgac cgcgtcacgg
     4321 agcttgatga cctcgtcctc ggtcaggtcc ttgacctggg tgttgcggtc gatgccgacc
     4381 tggtcgagaa tctcctgggc ggtcgactga ccgatcccgt aaacgtaggt gaggccgatt
     4441 tcggcccgct tgttgagggg aatgtttacc ccggcgatgc gcgccatcta ctaaccctgc
     4501 ctctgcttgt gtcgcgggtt ctcacagatc accaggacgc gtccgcgccg gcgaataatc
     4561 ttgcacttct cacacatcgg ttttaccgag gctcggacct tcatcgtcct aacgttcctt
     4621 ttggagagat gtcccgagaa tcccagtaaa cggactccgg gcgtgaaaaa agggccaatt
     4681 ttggccgctt gggaacaata gcaggctgcg aacattcggg accggccaga tcgcagaatc
     4741 cacgattgtc cagaatattc ggggaaaacc ggtgtttctc tggtcccatc ggaacttcta
     4801 ccggtcccag ggggtgagga ttcgggggcc gtccttggtg gcggcgaccg tgtattcgnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt cccagggggt gaggattcgg
     4981 gggccgtcct tggtggcggc gaccgtgtat tcgaaatggg cggccatcga gccgtcgtcg
     5041 gagaagaccg accagccgtc atcgtccatg tagacgtcgg ggccaccgac gttgaccatc
     5101 ggttcgatcg cgaagaccat tccctcctcg atctcggggc cgcggccggg agtgccgtaa
     5161 ttggcgatct gcggctcttc gtgcatgttc tgccccacgc cgtgaccgac cagggaacgg
     5221 atgatcgaga agccgttcgc ctcgacgtgg ctctggaccg cgtggccgat gtcccccacc
     5281 cggttgccgg gcaccatctg cgcggcggcg aggtcgagcg actccttggt cacggtgagc
     5341 agcttctgcg tggtctcgtc gatcgggccg accgggatgg tcgaggcgcc gtcggcgacc
     5401 cagccgtcga gggtaacccc gatgtcgatc gagaggatgt cgcccttctg aagcttgtag
     5461 ggcccgggga tgccgtgaac gaccatcgag ttgggggagg cgcagatcga accggggaaa
     5521 ccgcggaaac ccttgaaggt gggcaccccg ccctgggagc gaatgaactt ctcggccacc
     5581 tcgtcgagtt cggccgtggt cacgccgggc cgcaccttcg cggtcagcat cttcatgcac
     5641 cgcgcgtgga cggcacccgc ggccgccatc ttctcgatct gatcgtccgt cttgcggatg
     5701 atcatggggg tgagatcagc tgccttcttc gaggctgagc gttgaaacca gggtgcggat
     5761 cttctcgctg acccggtcgg gatcttcggc gccctcgacc ttgttcagca gaccctgctt
     5821 cttgtagtaa tcgaccagcg gcgcggtctt ttcctggtac tgctcaagcc ggtgccggac
     5881 cacttccggc ttgtcgtcat cacggatgta gagcggggag ccgtcgacgt cacagacgcc
     5941 ttcctgctcg ggcgggctgt tgtccacgtg gtagacatgg cccttctcct ggcagacgcg
     6001 gcggccggaa aggcggttga cgatctcatc gtcctcgacg tcgatcagga tcaccgccgt
     6061 gagctggcgg cccagcttgt gaagttcggc gtcaagcgcc tcggcctgca cgaccgtgcg
     6121 ggggaagccg tcgaggatga aaccgtgatc ggcctcgccg gactcgagcc gctcggcgat
     6181 gatgccgatg atcacctcgt ccggaaccag gtcaccccgg tccatgtact ccttcgcggc
     6241 gttgccgacc tcggtgccct ccttgaccgc ggcccggagg atgtccccgg tcgcgtagta
     6301 cgggtactcg agatcttcct gcagacgctc gccctgggtg cctttaccgc tcccgggcgg
     6361 gccgagcagg atcaggttga gttccgccac gggtaacccg ctacctgagg aagccctcgt
     6421 agttgcgcat catcagctgc gcctcgagct gcttgaccgt gtcgagggcc acgcccacga
     6481 cgatcaggat cgaggtgcca ccgaagaaga acccgccgcc cacgttgttg aagaggatgg
     6541 tcgggaaagc cgcaacggcg ccgaggtaga gggcgccagg gaaggtcagg cgggagagca
     6601 cgcggtccag gtattcggcg gtcggccggc ccgggcgcac gccgggaatg aacccgccgt
     6661 acttcttgag gttgtcggcc tggtccaccg gattgaaggt gacggccgtg tagaagtagg
     6721 tgaagatgat gatgaagaac acttcaccga ccacgtaggc ccagctcgac ggactgagga
     6781 aggcggcgaa gtcaagcgcg gcgggagtgt tgatcagctg accgatggtc ggcggcaggg
     6841 ccatgatcga ggcggcgaag atgaccggga tgacgccggc catgttgacc cggagcggca
     6901 ggtaggtcga accgcccgag gtcatcttgc gaccgaccac gcgcttggcg tactgcaccg
     6961 ggatccggcg ctgaccttcc tggacgaaga cgatcgcgac gatcacggcc aggcagatga
     7021 acggcagcag cacgacgaag acctgatcgg ggttgttcca ccaggtctgg atgccgggca
     7081 ggacgccgga gacgatcgag gcgaagatca ggag
//
LOCUS       DAOQKN010000135         7089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_5238_length_7089_cov_5.975405, whole genome shotgun sequence.
ACCESSION   DAOQKN010000135 DAOQKN010000000
VERSION     DAOQKN010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 7089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7089
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5238_length_7089_cov_5.975405"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="PLJ64_09090"
     CDS             complement(<1..374)
                     /locus_tag="PLJ64_09090"
                     /inference="COORDINATES: protein motif:HMM:NF025041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA N-terminal domain-containing protein"
                     /protein_id="HNC93676.1"
                     /translation="MAEVRMWGRRIGAVALADGARAAEFEYTPEFARSGIEVAPVEMP
                     LVEGRRYSFPELSTESFRGLPGMLSDSLPDRFGNALIDAWLARQGREPGSMGAVERLC
                     YTGRRGMGALEYLPATGPEPVE"
     gene            complement(400..750)
                     /locus_tag="PLJ64_09095"
     CDS             complement(400..750)
                     /locus_tag="PLJ64_09095"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNC93677.1"
                     /translation="MEYSRTDHAILEQLGTDLQRERLSRNLSQASLAAEAGITRDTVR
                     RLEAGEPVSTLTLVRVLRALGRAESLSGLLPGGGPGPLELFEKGPRGRRRARASSRDD
                     SRRSEWRWGDEDES"
     gene            860..2059
                     /gene="dinB"
                     /locus_tag="PLJ64_09100"
     CDS             860..2059
                     /gene="dinB"
                     /locus_tag="PLJ64_09100"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF003015.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNC93678.1"
                     /translation="MSTASILHADLDAFYASVEQRDDPALRGRPVIVGGGVVLAASYE
                     AKAKGIYTTMNGRDARRVCPEAAVVQPRMEAYSKASKAVYEIFNDTAPVVEGISIDEA
                     FLDVAGMEHIGGTPPEIAARLRSRVLAEAGLPISVGVARTKFLAKVASGASKPDGLLV
                     VEPDREAEFLHPLPIEALWGVGKVTTAKLNGMGIRTVGDVARTSRVRLAGVIGESAAA
                     HLLDLANGRDPRPVSPRERRKSIGAQSAFPRGSRDRSEVDSLAAALVDRTARRLRAAE
                     RACRTVSIGLRFGDFSRASRARTLAFPTDQTETILAAVRELFDASREEIAEKDLTLIG
                     ISLGNLEAADTVQMELPLSENDRLVENSALDQAVDDVRARFGEGVIKRASMIDADQGV
                     PVPTLPD"
     gene            complement(2066..6073)
                     /locus_tag="PLJ64_09105"
     CDS             complement(2066..6073)
                     /locus_tag="PLJ64_09105"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNC93679.1"
                     /translation="MKSSGWGKRAWGAVIGGLALAVFAGAAGQSASAARVQADRPAPQ
                     TFLLKLDEPSTVRVFRQNRDQGLPEARAAAKDQKSDITGAQSEVIDDLPANADPIYRT
                     HSVLAAVGVTAPAGDKTLLEQIPGVSAVYPVAPKSPTNDYAVPFQAAAQAWQSTGFLG
                     QGQTIAVIDTGVDYTHSNFGGPGTETAFETAQATEDQPANPALFPNAKVSGGIDLVGD
                     DYDSDSDSENYQPDPHPDPNPLDCGGHGSHVAGSAAGYGVKADGSTYDGVYDNSTDFA
                     AMKIGPGMAPRAKVYAIKVFGCDGSTDVVTEAIDRAVDPNNDGDPTDHVDVINMSLGS
                     DFGSVQDGDSVASNAAVQMGVSVVASAGNSGDISDITGSPGDASQVLSVASSVDAESK
                     VDGAEVTIDGNQDLYAITRSGRYDWKTGPDLSGPVVAAPVENETACAPYTGTPFTGKV
                     VLVKWHDAVPECGSITRGDNLAAAGAIGFIFGSDAETFSAGINGDDAIPGVLMAKSGA
                     DAIRDALDNSLAVSVDGTEVNAVTRSFPDDNDKISSFTSRGIHATGNVKPDVTAVGSS
                     VFSTAVGTGDDGVSFSGTSMASPMVAGLAALVRQANPGWTPLQVKADIMNTAAHDLFV
                     DGSANPSSDRYGPPRVGSGRIDAALATGNRVLAYDPANGAVSVSFGPVAASAPVTLSR
                     EVKVENQSSFPVTYDLAYDPINEVPGAEFSVTPSQVSLDPGRSTMVSVSLAIEDPSQL
                     TKAVDPTAGRFSSEGDPRETLAEAFGRLLLEPTGPGTTLRVPVYASPRPASDMGQPAS
                     LLIHRSSATAGDPEQTASLELAGQGLGSGPGENGVGDGDPDNDIFSIAAGFELQATSG
                     ESPECDEEIETSCWRLPEEKYADLKLVGYTSNAPLVADPDQQLAYFAAAVQAPWAIPA
                     DKAAIQFDLDVDGDGTPDLFLYNDRLGEDDVFVSTLLDPSRPEGQRIIDQQLVNGRFG
                     DIDTALYDSDALVLPVSLAALADYGIDADNPRVSYGVETYSYSSDQAIDMIGVNAESG
                     DLENPLSANLFEPGILVTGDGGVGPLVEDQPGEQLTVTRNVASYRDDAGLGVLMLHFH
                     NPVGDKAQVVALTGAESATELSTTPGKLTAKVKAVGDGLPEPSGEVTFSVDGTEVGSA
                     TVEDGSAILKAGTPHGGVHQIKAEYEGDADFAASSDQFERTDPGLTARIKTPKSSFGW
                     YRKPVDIYFRCVTHGSELTQDCPYPRRLGRDGRGQQVKASITALDGGKATVTVSGINI
                     DRTAPRVKISGVRRGASYPRIRRARCVGRDRLSGLASCGIRWKRRGKRVIYTATGVDR
                     AGNRRTVRTWVRITR"
     gene            complement(6185..6643)
                     /locus_tag="PLJ64_09110"
     CDS             complement(6185..6643)
                     /locus_tag="PLJ64_09110"
                     /inference="COORDINATES: protein motif:HMM:NF040341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6174 domain-containing protein"
                     /protein_id="HNC93680.1"
                     /translation="MKKLAITAVLLICGLTASSALAADEDQVDPDITSGKAAREFRQA
                     RQKWLAAGIVDYRMKVSRGCFCAGPSSVTITVRGGKAVKVSDKPWYGPWTVPGMYRII
                     GQAIKNEVAALDVTYGAKLGIPRQTSIDYIAMAVDDEIGYGISGFKALKP"
     gene            6692..>7089
                     /locus_tag="PLJ64_09115"
     CDS             6692..>7089
                     /locus_tag="PLJ64_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNC93681.1"
                     /translation="MPYLHKWSPALVRTLPHTRLGTGPTPVRPLPSLGQSGCEIWLKD
                     ESGFGDGGWGGNKVRKLEWLLPDVKRRGRKTILTFGGLGTNWGLATALYGREQGIHTA
                     LGLIDQPRDQHVEEQLKRLEASGASLHFTHT"
BASE COUNT         1119 a         2408 c         2379 g         1183 t
ORIGIN      
        1 ctctcgaccg gctccggacc ggtcgccggc aggtactcga gtgcccccat tccccggcgg
       61 cccgtgtaac agagccgctc gaccgctccc atgctgccgg gctcgcgacc ctggcgggcc
      121 agccaggcgt cgatcagggc attgccgaag cgatccggca gcgaatccga aagcatgccg
      181 ggaagcccgc ggaagctctc cgtcgaaagc tccgggaagc tgtagcggcg gccttctacc
      241 aggggcattt caaccggagc cacctcgatc ccgctgcggg cgaactccgg cgtgtactcg
      301 aactcggcgg ctctcgctcc gtcggcgagc gccaccgcgc cgatgcgccg gccccacatt
      361 cgaacctcgg ccacgctggt cattactcag cccggccgtt catgactcgt cctcgtcgcc
      421 ccatcgccac tcggaccgtc ttgaatcgtc gcggctcgag gcccgggccc tgcgccggcc
      481 ccgcggaccc ttctcgaaca gttcgagcgg gcccggccca ccacccggga gcaggccgct
      541 cagtgattcg gcccgcccca gggcccgcag aacccggacc agggtcaggg ttgaaaccgg
      601 ctcgccggcc tcgagccggc gcaccgtgtc ccgcgtgatg ccggcttcgg cggcgaggct
      661 ggcctggctc aggttgcgcg aaaggcgctc gcgctgcagg tccgttccca gctgctcgag
      721 gattgcatga tctgttcgac tgtattccat agtgactgaa ctatcagtca ctatatcagt
      781 ctcggcacct tatgactgat atttcagtca ctaataaggg ccgagtcttg ccgacagcct
      841 ggcctgcgaa catgggttcg tgagcacggc ttcgatcctc cacgccgacc ttgacgcttt
      901 ttacgcctcg gtcgaacagc gcgacgaccc cgccctgcgg ggaaggccgg taatcgtcgg
      961 tggtggcgtc gtcctggccg ccagctacga agccaaggcg aagggcatct acacgacgat
     1021 gaacggtcgc gatgcccgac gggtctgccc cgaagcggcg gtcgtccagc cccggatgga
     1081 ggcctattcg aaggccagca aggcggtcta cgagatcttc aatgacacgg cgccggtggt
     1141 cgaagggatt tcaatcgacg aggccttcct cgatgtggcc gggatggaac acatcggggg
     1201 cactccgccc gagatcgcgg cccggctcag gtcacgggtc ctggccgagg ccggcttgcc
     1261 gatttcggtc ggcgtcgccc ggaccaagtt cctggcaaag gtggccagcg gagcttccaa
     1321 gccggatggc ctgctggtgg tcgaacccga tcgggaggcc gagttcctcc accccctgcc
     1381 gatcgaggcg ctctggggag tcggcaaggt gaccaccgcg aagctgaacg ggatgggcat
     1441 ccgtacagtc ggtgacgtgg cccggaccag ccgggtgcga ctggccgggg tgatcggcga
     1501 gtccgctgcc gcacatctgc tcgatctcgc caacggacgc gaccccaggc cggtctcgcc
     1561 gcgcgagcgg cgcaagtcga tcggggccca gagcgcgttc ccgcggggtt cccgagaccg
     1621 gtccgaggtc gattcactgg ccgccgccct ggtcgatcgc accgcccgcc ggctccgggc
     1681 ggcagagcgc gcctgccgca cagtctcgat cggcctccgc ttcggtgatt tctcgcgcgc
     1741 gagccgggcc cgcacgctcg ccttcccgac cgaccagacc gaaacgatcc tcgcggcggt
     1801 ccgggagctc ttcgatgcct cacgcgagga gatcgcggaa aaggacctca ccctgatcgg
     1861 catttccctg ggcaatctcg aagcagccga caccgtccag atggaactgc cgctcagcga
     1921 aaacgatcgc ctggtcgaga attccgccct cgatcaggcg gtcgatgacg tccgggccag
     1981 attcggcgaa ggggtgatca aacgggcgtc gatgatcgac gccgaccagg gcgttcccgt
     2041 gccgaccctg ccggactgaa ggacctcagc gggtgatccg gacccaggtc cggacggtcc
     2101 ggcggttgcc ggcccggtcg acgccggtcg cggtgtagat gacccgtttg ccgcgacgct
     2161 tccagcgaat gccgcagctc gcgagacccg aaagcctgtc ccggccgacg caacgcgccc
     2221 ggcgaatccg cgggtaggag gctccgcgcc ggacgccgct gatcttcacc cggggcgcag
     2281 tacggtcgat gtttatgccg ctcaccgtga ccgttgcctt gccgccgtcc agggcggtga
     2341 tcgaggcctt gacctgctgg ccgcggccat ccctgcccag gcgccggggg taggggcagt
     2401 cctgggtcag ctccgacccg tgggtgacgc aacggaagta gatgtcaacc ggcttgcggt
     2461 accacccgaa gctgctcttc ggagtcttga tccgggccgt cagaccgggg tcggtccgct
     2521 cgaactgatc cgaggaggcc gcgaagtcgg cgtcgccttc gtactcggcc ttgatctggt
     2581 gaaccccgcc gtgaggcgtg ccggccttga ggatcgcgct cccgtcctcg accgtcgccg
     2641 atccgacctc ggtgccgtca accgagaagg tcacttcgcc cgaaggctcg ggcaggccgt
     2701 caccgacggc cttgaccttt gcggtcagct tgccgggcgt ggtcgagagc tcggtggccg
     2761 attcggcccc ggtcagggcc acgacctggg ccttgtcccc gaccggattg tggaagtgga
     2821 gcatcagcac cccgaggcca gcgtcatccc ggtaggaagc aacgttcctg gttacggtca
     2881 gctgctcgcc gggctggtcc tcgaccagcg gtcccacgcc gccgtcaccg gtgaccagga
     2941 tgcctggctc gaagagattc gcgctgagcg gattctcgag gtcgccgctt tcggcattga
     3001 cgccgatcat gtcgatcgcc tgatcggagg agtacgagta ggtctcaacc ccgtatgaaa
     3061 cccgggggtt gtctgcgtcg atgccgtaat cggccagcgc ggccagtgaa acgggcagca
     3121 ccagcgcgtc gctgtcatag agcgcggtgt cgatgtcccc gaagcgcccg ttgaccagct
     3181 gctggtcgat gatccgctgg ccctccggcc gggacggatc gagcagggta ctgacgaaga
     3241 catcgtcctc cccgaggcgg tcgttgtaga ggaagaggtc cggcgtgccg tccccgtcga
     3301 cgtcgaggtc gaactggatc gcggccttgt ccgccgggat cgcccagggc gcctgaaccg
     3361 cagccgcgaa gtacgccagc tgctgatccg ggtcggcgac cagcggggcg ttcgaggtgt
     3421 agccgaccag cttcaggtct gcgtacttct cttccggcag ccgccagcag gaggtctcga
     3481 tctcctcgtc gcattcgggc gactccccgc tcgtcgcctg cagttcgaag cccgcggcga
     3541 tcgaaaatat gtcgttgtcc gggtcgccgt cccccactcc gttttcaccg gggccgctgc
     3601 cgaggccctg gccggccagc tcgaggctcg cggtctgctc gggatcgccg gcggtggcgg
     3661 agctgcgatg aatcagaagc gaagcgggct ggcccatgtc cgaggccggc cggggagatg
     3721 cgtaaaccgg gacccgtagg gtcgtgccgg ggccggtcgg ctcgaggagc agccgcccga
     3781 acgcctcggc cagggtctcg cgcgggtcac cctcggagct gaagcggccg gcggtcggat
     3841 cgaccgcctt ggtcagctgg gaggggtcct cgatcgcgag gctgaccgag accatggtcg
     3901 acctgcccgg atccaggctg acctgggaag gcgtaaccga gaattcggcg cccggaacct
     3961 cgttgatcgg gtcataggcg aggtcgtagg tcaccgggaa ggaagactgg ttctccacct
     4021 tgacctcacg actgagggtc accggtgcgc tggcggcgac cgggccgaag gacacactga
     4081 ccgctccgtt ggccgggtcg taggccagca ccctgttgcc ggtggcgagt gccgcgtcga
     4141 tccggccgga accgacccgg ggcgggccgt agcgatccga ggacggattg gccgaaccgt
     4201 cgacgaagag gtcgtgcgca gccgtgttca tgatgtccgc cttgacctgg agcggagtcc
     4261 agcccggatt ggcctgccgg accagggccg ccagcccggc gaccatcggc gaagccatcg
     4321 aggtgccgga gaagctgacc ccgtcgtcac ctgtgccgac cgcggtcgag aagaccgagc
     4381 tgccgaccgc ggtcacgtcc ggcttgacgt tgccggtcgc gtgaattcca cgcgaggtga
     4441 agctcgagat cttgtcgttg tcatcaggga agctccgggt taccgcgttg acctcggttc
     4501 catcgacgct caccgccagc gagttgtcga gcgcgtcgcg gatcgcgtcc gcccccgatt
     4561 tcgccatcaa cacgccgggg atcgcgtcgt caccgttgat tccggcggag aaggtttccg
     4621 cgtcgctgcc gaagatgaag ccgatcgctc cggccgcggc gaggttgtcg ccacgggtga
     4681 tcgaaccgca ttccggcacc gcgtcatgcc atttgacgag caccaccttg ccggtgaacg
     4741 gggtcccggt gtacggggcg caggcggttt cattttccac cggagcggcc accaccggcc
     4801 cgctgaggtc gggacccgtc ttccagtcgt aacggcccga ccgggtgatt gcgtacagat
     4861 cctggttccc gtcgatcgtg acctcggcgc catccacctt cgactcggcg tcaacgcttg
     4921 aggccacgct cagaacctgc gaggcatctc cgggcgagcc ggtgatgtca gagatgtcac
     4981 cggagtttcc ggccgaggca accacgctta cccccatctg aaccgcggcg ttggaggcga
     5041 ccgaatcccc gtcctgaacc gatccgaaat cggagccgag cgacatgttg atcacgtcga
     5101 catggtcggt cgggtcgccg tcgttgttcg ggtcgaccgc gcggtcgatc gcctcggtca
     5161 ccacgtcggt gctgccgtcg cagccgaaga ccttgatcgc gtagaccttc gccctgggcg
     5221 ccatgcccgg accgatcttc atcgccgcga agtcggtgga gttgtcgtag acgccgtcat
     5281 aggtcgagcc gtcggccttg acgccgtatc cggcggccga gccggccacg tgggagccat
     5341 gaccgccgca gtccagcggg ttcggatcgg ggtgagggtc gggctggtag ttctcggaat
     5401 cggaatcgga gtcatagtcg tctccgacca ggtcgatccc gccggagacc ttggcgttgg
     5461 gaaacagggc cgggttggcg ggctggtcct cggtcgcctg ggcggtctcg aaggcagtct
     5521 cggtgccggg accgccgaag ttcgagtggg tgtagtcgac gccggtatcg atcacggcga
     5581 tcgtctggcc ctggccgagg aagccggtcg actgccaggc ctgggcggcc gcctggaacg
     5641 gcaccgcgta gtcattggtc gggctcttgg gcgccaccgg gtagaccgcg ctgacgccgg
     5701 ggatctgttc gagcagggtc ttgtcaccgg ccggggcggt tacgccgacc gcagccagca
     5761 cgctgtgggt ccggtagatc gggtcggcgt tcgcggggag gtcatcgatc acctcgctct
     5821 gggctccggt gatgtccgac ttctgatcct tcgccgcggc ccttgcctcg ggcaggccct
     5881 gatcgcggtt ctgccggaac acgcggacgg tcgaaggttc gtcgagtttc agcagaaagg
     5941 tctgcggcgc cgggcggtcg gcctgaacac gggcggcgga agcgctctgg cctgcggctc
     6001 cagcgaacac ggccagcgcc aatccaccga tgacggcgcc ccacgccctt ttaccccacc
     6061 cgctgctctt catgccgccg acctttgctc gaattcagat gcctgacggg tcagactgcg
     6121 acccggaccg ccgtgagtct aaaccccgct ccgggccgaa agtggcggtc tgcgccgcgc
     6181 tggctcaggg cttcagcgcc ttgaagccgg agatcccgta gccgatctcg tcgtcgaccg
     6241 ccatcgcgat gtagtcaatc gaggtctgcc tgggtatgcc cagcttcgcg ccgtaggtga
     6301 cgtcgagcgc ggcgacttca ttcttgatcg cctggccgat gatccggtac atgccgggca
     6361 cggtccacgg cccgtaccag ggcttgtccg agaccttgac tgccttgcca ccacgaacgg
     6421 tgatggtcac gctggaagga ccggcgcaaa aacagccccg ggaaaccttc atccggtagt
     6481 ccacgatgcc cgcggcaagc cacttctgcc gggcctgcct gaactccctg gccgccttgc
     6541 cgctggtgat gtccgggtca acctgatcct cgtcggcggc gagggccgag ctggccgtca
     6601 ggccgcaaat caggagaacg gcggtgatgg cgagtttctt catggccata ttcttactac
     6661 gaaaccgatc ggcaaccgtc acaatcgcgc cgtgccctat ctccacaagt ggagccccgc
     6721 cctggtgcga accctccccc acacgcgact cggcaccgga ccgacgccgg tcaggcccct
     6781 gccctccctc gggcaaagcg gctgcgagat ctggctcaag gacgaaagcg gcttcggtga
     6841 cggaggctgg ggcggcaaca aggtccgcaa actcgagtgg ctccttcccg acgtcaaacg
     6901 gcgcggccgg aagacgatcc tgaccttcgg cggcctcggc accaactggg gcctcgcgac
     6961 cgcgctctac gggcgcgagc agggaatcca cacggccctc gggctgatcg accagccccg
     7021 cgaccagcac gtcgaggagc agctgaagcg cctggaagcc tccggggcaa gcctccactt
     7081 cacccacac
//
LOCUS       DAOQKN010000136         6785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_5600_length_6785_cov_5.849183, whole genome shotgun sequence.
ACCESSION   DAOQKN010000136 DAOQKN010000000
VERSION     DAOQKN010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 6785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6785
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5600_length_6785_cov_5.849183"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..1145
                     /locus_tag="PLJ64_09120"
     CDS             18..1145
                     /locus_tag="PLJ64_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC93682.1"
                     /translation="MSRRVLILGAGFGGLELGTTLSEELGDQVEVTLIDRNESFSFGY
                     SKLDIMFRGASRDSVSLPYSAVAKTGVTLLREEITAIDPETRTVTTDAGTHQGDALVI
                     ALGADYDLDSTPGLAERGHEFYSFAGAEAAAGPIAGFDSGKAVIAVCGAPFKCPPAPS
                     ECALMLDDELTRRGVREQCEITLTMPLGTPVPPSPETSAALKAEFGQRGITFLGDMRP
                     KRVEPDRIIYQDGTELPFDLLLGVPKHRAPEVVIDSGITEDEYVPVDSATLKTRFEGV
                     YAVGDVATAGVPKAGVFAEGAAKVVARELVAEIRGEASPEPYDGRGSCYIEFGEDRIA
                     RVDIDFLSGPEKTGVFNAPSLDLRKEKAEFGSSRKSRWFNR"
     gene            1171..2841
                     /locus_tag="PLJ64_09125"
     CDS             1171..2841
                     /locus_tag="PLJ64_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93683.1"
                     /translation="MSLGRGFYRLLGSLVCAIAFFAGVGNASAELIDSEDLGDGVQRL
                     TYRIGPIEVTSGQNRIVYKMVTAEERPPVDGYITAFRPNLVNPDGTIPKSSSIMFHHG
                     VWINLSRRDATSGGMSERFIGTGEEKTEVVFPPGFGYFHKGTDVWLLNHMIHNLTPAE
                     HTLYITYQIDFVPLTSPKAEGMRGVRPIWMDVMNPNPYPVFDTYKGSGGKDGKIVWPY
                     EAKEDPYKDGIKRNLWTVDRDGVLVWSVGHVHTGGLATDLYLNRDGAKYAGPKCPKPR
                     ILRKKLKKNAPRSLRSARSKAAVKYRKCLARMPDVKGDRVHLFKSNAHYYEPAGPVSW
                     DMAMRNTRKDWLVQLKAGDTLEVTTTYETKRASWYENMGIAISYMADGTGGKDPYRTR
                     MDYTGPINHRHYSENNDHGGKLPVVGPDPTGLENGPFASDPFVISGYSYGQGDYRLPG
                     AAGRPPVVEKGDTFTFSLSDGDVGNKIWHSLTSCAAPCNRSTGIAYPIADGDFRFDSG
                     QMGVGGAPTVNRTTWTTPANLPVGTHTFFCRIHPLMRGAIRVVPKGSG"
     gene            2838..3335
                     /locus_tag="PLJ64_09130"
     CDS             2838..3335
                     /locus_tag="PLJ64_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93684.1"
                     /translation="MIAAVPLSEWSDFFVASAGASAALAGLVFVAISINVARILQLDG
                     VPELGLVTLLLLIGVLVVSMFGLIPDQSEHAFGVELLVQSAFWTLVIAFLIRRSAAGL
                     GEGGGRLASRVILPVFGTLPYLAGAVLLITGSDIAMYWVFAGMLGAILAAVMNAWILL
                     VEILR"
     gene            complement(3398..5302)
                     /locus_tag="PLJ64_09135"
     CDS             complement(3398..5302)
                     /locus_tag="PLJ64_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93685.1"
                     /translation="MEIGKAESSAVPGRWFIAAGVFVAAVLTLLAVASGARAEILKTE
                     VLPGGVQRTTYRIGPVDVTPGQNRIAYRPIEGASRPAVDGWITRIKPDLVYEDGSIPD
                     SSKVMFHHGVWINLSTNEQFYATGEEKTILDLPEGFGYRYKTDEGWLLNDMIHNLTPQ
                     AMKLYFEYTLDFIPDTAAEAAGIVRARPIWMDVQNGSIYPVFDVWRDSGGDDGEFTYP
                     EDATNPYPPGNQKNVKSIPANGMLIATTGHVHTGGLSTDLYLRRDGATYQGPNCPDRI
                     SYEDELKPLQQKNDSLVARINKLKQKNAKMTRTQQKIRRSMKNRQSRSKAKSLIKDIK
                     RRKKSNSKTIAMVNRLRAKNKIQLDKVKAMDKAERERDAACRGTQPSVDDGNRVHLFK
                     STADYFDPRGPISWDMAMVSTDNDWRVRVKAGDKLELQTTYETKRASWPESMGINIVY
                     WVRDSAYAAAANAPDPYVTNVAQKGVLNHGHLKENEDYGGETGKNGSKLVGPDPTKLP
                     DGPETGGPFTISGFSYNQGDFRLPGSAGRPPVVPQGQSFTFKLSDSDITNEVWHSITS
                     CAAPCNKSTGISYPIPNGEVQFESGQMGPGKAPTVGYTQWSTPSDLKKGTYTFFCRIH
                     PLMRGAFRVK"
     gene            complement(5411..>6785)
                     /locus_tag="PLJ64_09140"
     CDS             complement(5411..>6785)
                     /locus_tag="PLJ64_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNC93686.1"
                     /translation="SPKNSPLTVAVPGDGLISGTATVNGEFFGENSRCAVLSYDYTVL
                     AGTQGAVGHLKKDRLFELIEKMKLPTIFFAEGGGGRPGDTDFPVVSALQVRAFALWAG
                     LSGLVPRVAIVKGRCFAGNAVIAGCADLIVATKDSSLGMGGPAMIAGGGLGEVAPDEV
                     GPVSFQEPNGVLDLVVADEAEATAAAKKLIGYFQGRSPWTDGGESDQAPLREMVPERE
                     RRAFDVRPVIETIADPDSVTLLREKFAPELVTALARIEGRPVGVIANDSRHMAGTITS
                     DSADKAARLLQLCESFGLPVISLVDTPGFMVGPAHEQTALVRHTSRLLVAGAALTVPL
                     ISVILRRAYGLGAQAMVGGSLHEPLLTVAWPSAHLGPMGLEGAVRLAMRKDLEAIADE
                     EEREKRVRELTALAEQNARAMNAAQLFEIDDVIDPAETRPVIASTLAAAPERVSERAP
                     HRFVDTW"
BASE COUNT         1134 a         2212 c         2237 g         1202 t
ORIGIN      
        1 ggcagtatgc tggccccgtg agcagacggg tactgattct gggagcgggc tttggcggac
       61 tcgaactggg cacgacgctc tccgaagagc tgggggatca ggtcgaggtc accctgatcg
      121 atcgaaacga gtcgttctct ttcggctact cgaagctcga catcatgttc cgcggggcca
      181 gccgggactc ggtcagcctg ccctattcgg cggtggccaa aaccggggtc accctcctca
      241 gggaagagat cacggcgatc gacccggaaa cccgcacggt cacaaccgac gccggaaccc
      301 atcagggaga tgccctggtc atcgcgctgg gcgccgatta cgacctcgat tcaaccccgg
      361 gcctggccga acggggacac gagttctatt ccttcgccgg ggccgaagcc gcggccgggc
      421 cgatcgccgg cttcgattcc ggcaaggcgg tgatcgcggt ctgtggcgct cctttcaagt
      481 gcccgccggc cccgagcgaa tgcgccctga tgctggacga cgaactgacc cggcgcgggg
      541 ttcgcgagca atgcgagatc accctgacca tgccgctcgg cacaccggtg ccaccctcgc
      601 cggaaacctc cgccgccctc aaggcggagt tcgggcagcg gggcatcacc ttcctcggtg
      661 acatgcggcc caagcgggtc gaaccggaca ggatcatcta ccaggacggg accgagcttc
      721 ccttcgacct gctgctcggc gtgccgaagc accgggcccc ggaagtggtg atcgattccg
      781 gcatcaccga agacgaatac gtgccggtcg actccgccac cctgaagacc cgcttcgaag
      841 gggtctatgc ggtcggggac gtcgccacgg ccggcgtgcc caaggcggga gtgttcgccg
      901 agggggcggc gaaggtagtc gcccgcgaac tcgtggcgga gatccggggc gaggccagcc
      961 cggaacccta cgacggcagg ggctcctgct acatcgaatt cggcgaggac cggatcgccc
     1021 gcgttgacat cgatttcctc tccgggccgg aaaagaccgg ggtcttcaac gcaccgtcac
     1081 tcgaccttcg caaggagaag gcagagttcg gttcgagccg caaatcacgc tggttcaacc
     1141 gataactgat actggcgcta ctctttcccc atgtccttgg ggagagggtt ttacagactg
     1201 ctgggcagcc tcgtctgcgc aattgcgttc ttcgccgggg tcggcaacgc ctcggcggag
     1261 ctgatcgatt cggaagatct gggtgacggc gtccagcgcc tgacctaccg gatcggtccg
     1321 atcgaggtca cttccggcca gaaccggatc gtctacaaga tggtcaccgc cgaggagcgg
     1381 ccgccggttg acgggtacat cacggccttc cggccgaacc ttgtcaaccc ggacgggacc
     1441 atcccgaaat cgagcagcat catgttccac cacggggtct ggatcaacct ctcccgtcgc
     1501 gacgccactt cgggcggaat gtccgagcgg ttcatcggca ccggcgagga gaagaccgag
     1561 gtcgtcttcc cgcccggctt cggttacttc cacaagggga ccgacgtctg gctcctcaac
     1621 cacatgatcc acaacctgac gccggccgaa cacactctct acatcaccta ccagatcgat
     1681 ttcgtcccgc tgacttcacc aaaggccgag gggatgcgcg gcgtgcgccc gatctggatg
     1741 gacgtgatga acccgaaccc ctatccggtc ttcgacacct acaagggatc gggtggcaag
     1801 gacggcaaga tcgtctggcc ttacgaggcc aaggaggatc cctacaagga cgggatcaag
     1861 cgcaacctct ggaccgtcga ccgggacggc gtgctggtct ggtcggtcgg ccacgttcac
     1921 accggcggcc tcgcgaccga cctttatctg aaccgcgacg gcgcgaagta cgcgggcccg
     1981 aagtgcccga agccgcggat cctcaggaag aagctgaaga agaacgcccc ccgctcgctg
     2041 cggagcgcgc ggagcaaggc cgcggtgaaa taccgcaagt gcctggccag gatgcccgat
     2101 gtcaaaggtg accgggtcca tctcttcaag tcgaatgccc actactacga gccggccgga
     2161 cctgtttcct gggacatggc gatgcgcaac acgcgcaagg actggctggt ccagttgaag
     2221 gccggcgaca cgctggaagt caccacgacc tacgaaacga agcgtgcctc ctggtacgag
     2281 aacatgggca tcgcgatctc ctacatggct gacggcaccg gcggcaagga cccgtacagg
     2341 acccggatgg actacaccgg gccgatcaac cacaggcatt actccgagaa caacgaccac
     2401 ggcggcaagc tgccggtggt cgggccggat ccgaccgggc tcgagaacgg gccgttcgcg
     2461 agcgacccct tcgtgatcag cggctacagc tacggccagg gcgactaccg cctgcccggc
     2521 gcggccggca ggccgccggt ggtggagaag ggtgacacct tcaccttctc gctctcggac
     2581 ggtgacgtcg gcaacaagat ctggcactcg ctgacttcct gcgccgcacc ctgcaacagg
     2641 tcgacgggga tcgcctatcc gatcgccgac ggggacttcc gcttcgactc cggccagatg
     2701 ggcgtcggcg gagcgcccac ggtcaaccgg acgacctgga ccacaccggc caatcttccg
     2761 gtcggcacac acaccttctt ctgtcgcatc cacccgctga tgcgcggtgc gatccgggtg
     2821 gtgcccaagg gcagcggttg atcgccgccg ttccgctttc ggaatggagc gacttcttcg
     2881 tcgcctcggc cggggcctcg gcggccctgg ccggcctcgt tttcgtggcg atctcgatca
     2941 acgtcgcgcg aatcctgcag cttgacgggg taccggaact cggcctggtc accctgctgc
     3001 tcctgatcgg ggtgctggtc gtctcgatgt tcgggctgat tcccgaccag tcggaacatg
     3061 ccttcggggt tgagctgctg gtccagtcgg cattctggac gctcgtcatc gccttcctga
     3121 tcaggcgaag tgcggccggt ctcggtgaag gaggcggtcg cctggcctcc cgggtcatcc
     3181 tgccggtctt cgggaccctg ccctatctgg ccggcgcggt cctgctgatc acgggcagtg
     3241 acatcgcgat gtactgggtt ttcgccggca tgctcggagc gatcctggcg gcggtgatga
     3301 acgcctggat actgctggtc gagatcctcc gttagagcac ggacgagccg gccccggagg
     3361 gaccggctcc tcggctcggc gggccgggcg accccggtta cttgacccgg aaggcgccgc
     3421 gcatcagcgg gtggatgcgg cagaagaagg tgtaggtgcc cttcttcaga tcgctgggcg
     3481 tcgaccattg ggtgtagccg accgtcggag ctttgcccgg tcccatctgg cccgactcga
     3541 actggacttc gccgttcggg atcggatacg agatgccagt cgacttgttg cagggtgccg
     3601 cgcaggaggt gatcgagtgc cagacctcgt tcgtgatgtc cgaatcggac agcttgaagg
     3661 tgaaggactg gccctgcggg acgaccggtg ggcgaccggc tgaacctggc aggcggaagt
     3721 caccctggtt gtagctgaaa ccgctgatcg tgaacggccc gccggtttcc gggccgtcag
     3781 gcagtttggt cggatcgggt ccaaccagct tgctgccgtt cttgccggtc tcgccaccgt
     3841 agtcctcgtt ctccttcagg tggccatgat tcagcacacc cttctgggcg acgttggtca
     3901 catacgggtc gggagcgttg gccgcggcgg cgtaggcgga atcacggacc cagtacacaa
     3961 tgttgatgcc catcgactcg ggccaggagg cccgcttcgt ctcgtaggtc gtctgcaact
     4021 ccagcttgtc cccggccttg acccggaccc gccagtcgtt gtcggtgctg accatcgcca
     4081 tgtcccagga gatcggaccg cgcgggtcga aatagtccgc ggttgacttg aacaggtgga
     4141 cgcggttgcc gtcgtcgacc gacggctggg taccgcggca ggcggcgtcg cgctcgcgct
     4201 cggccttgtc catggccttg accttgtcga gctggatctt gttcttcgcc cggaggcggt
     4261 tgaccatcgc gatggtcttc gagttggact tcttgcgccg tttgatgtcc ttgatcaggc
     4321 tcttcgcttt cgacctgctc tgccggttct tcatcgagcg ccggatcttc tgctgggtcc
     4381 gcgtcatctt cgcgttcttc tgcttgagct tgttgatccg cgccaccaag ctgtcgttct
     4441 tctgctgaag cggcttcagc tcgtcctcgt aggagatccg gtccgggcag ttcggcccct
     4501 ggtaggtggc gccgtcgcgg cgcaggtaca ggtcggtcga gagtccgccg gtgtggacgt
     4561 ggccggtggt cgcgatcagc atgccgtttg ccgggatcga cttgacgttc ttctggttgc
     4621 cgggcgggta ggggttggtc gcgtcctcgg gataagtgaa ctcgccatcg tccccgccgc
     4681 tgtcacgcca gacgtcgaag acggggtaga tgctgccgtt ctggacgtcc atccagatcg
     4741 gacgggcccg cacgatgccg gcggcttcag ccgcagtgtc cgggatgaag tcgagcgtgt
     4801 actcgaagta gagcttcatc gcctgcgggg tgaggttgtg gatcatgtcg ttcagcaacc
     4861 agccctcgtc cgtcttgtag cggtaaccga agccctcggg caggtcgagg atggtctttt
     4921 cttcgccggt cgcgtagaac tgctcgttgg tcgagaggtt gatccagacc ccgtgatgga
     4981 acatcacctt gctcgagtcg ggaatcgagc cgtcctcgta gacgaggtcc ggcttgatcc
     5041 gggtgatcca gccgtcgacg gctggccggg aggcaccttc gatcgggcga taggcaatcc
     5101 ggttctgacc cggggtcacg tcgaccgggc cgatccggta ggtggtcctc tgaaccccgc
     5161 cgggcaacac ctcggtcttg aggatctcgg cgcgggcgcc actggcgacc gcgagcaaag
     5221 tcaacactgc tgcaacgaaa actcctgccg cgatgaacca gcggcccggc acagcagacg
     5281 attcggcttt cccgatttcc atcaaccctc tcccttgact gcgtaaaaag acaacttcaa
     5341 gttaccccat aaccgcagag ggacctccgg gttgcgggtc tggccgaaag agaccggccc
     5401 gccgccggct tcaccaggtg tcaacgaagc ggtgcggcgc acgctctgag acccgttcgg
     5461 gcgcggcggc cagagtcgag gcgatgaccg gtctggtctc ggccggatcg atcacgtcat
     5521 cgatctcgaa gagctgggcc gcgttcatgg cccgcgcgtt ttgctcggcc agggcggtca
     5581 gctcgcgaac ccgcttttcc cgctcctcct cgtcggcgat cgcctcgagg tccttgcgca
     5641 tcgccaggcg tacggcaccc tcgagaccca tcggcccgag gtgggcgctc ggccaggcga
     5701 cggtgagcag gggttcgtgg aggctgccgc cgaccatcgc ctgcgcgccc agcccgtagg
     5761 cccggcgcag gatcaccgag atcagcggaa ccgtcagggc ggcgcctgca accagcaggc
     5821 gggaggtgtg ccggaccagg gcggtctgct cgtgggccgg cccgaccatg aagccgggag
     5881 tgtcaaccag ggagatgacg ggcaggccga agctctcgca aagctgaagc aagcgggccg
     5941 ccttgtcggc cgagtcgctg gtgatcgtgc cggccatgtg gcgggagtcg ttggcgatca
     6001 cgccgaccgg ccggccctcg atccgggcca gcgcggtgac cagttccgga gcgaacttct
     6061 cgcgcagcag ggtgaccgaa tccggatcgg caatggtctc gatcaccggg cgcacgtcga
     6121 aagcccgtcg ctcgcgttct gggaccatct cgcggagcgg agcctggtcg ctttccccgc
     6181 cgtcggtcca ggggctgcgg ccctggaagt aaccgatcag cttcttcgcc gccgcggtcg
     6241 cctccgcttc atcggcgacg acgaggtcaa gcacaccgtt gggctcctgg aacgaaaccg
     6301 gaccgacctc atccggggcg acctcgccca gacccccgcc ggcgatcatc gccgggccgc
     6361 ccatgccgag cgaggagtct ttggtggcga cgatcaggtc ggcgcagccg gcgatcaccg
     6421 cattgccggc gaagcagcgg cccttgacga tcgccaccct cgggaccagg cccgagagcc
     6481 cggcccaaag tgcgaaggcc ctcacctgca gggccgagac gaccgggaaa tcggtgtcgc
     6541 cggggcggcc gccaccaccc tcggcgaaga agatcgtcgg cagcttcatc ttctcgatca
     6601 gctcgaagag ccggtccttc ttgaggtggc cgaccgctcc ctgggtgccg gccagcaccg
     6661 tgtagtcgta ggagagcacg gcgcagcggg agttctcgcc gaagaattcg ccgttgaccg
     6721 tggcggtgcc cgagatcagt ccgtcgccgg gcaccgccac ggtcaacggc gaattcttcg
     6781 gcgag
//
LOCUS       DAOQKN010000137         6680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_5736_length_6680_cov_5.640151, whole genome shotgun sequence.
ACCESSION   DAOQKN010000137 DAOQKN010000000
VERSION     DAOQKN010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 6680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6680
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5736_length_6680_cov_5.640151"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="PLJ64_09145"
     CDS             complement(<1..184)
                     /locus_tag="PLJ64_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93687.1"
                     /translation="MPKPIGQSSQRYMPGLDGLRGVAVLAVIAYHLKIDWASGGLLGV
                     GVFFTLSGYLITDILLE"
     gene            309..896
                     /locus_tag="PLJ64_09150"
     CDS             309..896
                     /locus_tag="PLJ64_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNC93688.1"
                     /translation="MRRIDREIQSWSRDSARVQLALLWDEVERDGVRSISWWDGDPWA
                     SAYLISSTQPWSGGIGKWRAFCMINFGRPEDDEILFSDDREVVGRTSPAHGMGAREIR
                     RMLELPGVEERKELCDLLIALGHRRDELDIPTITGFAGHEDRVVRYVVLAMLSWNPSI
                     ANDYLAGKFSDDPDPLVRELARRWSGGRLRRRTRA"
     gene            complement(929..1369)
                     /locus_tag="PLJ64_09155"
     CDS             complement(929..1369)
                     /locus_tag="PLJ64_09155"
                     /inference="COORDINATES: protein motif:HMM:NF024861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HNC93689.1"
                     /translation="MTGSAKSDSSTRIACDTNVMVSAFIAGGPPGRVIEEAADGSLGL
                     VLLEPVLNELTRILPAKFDFDTAELRQLLDLLADISVDAQGPPAGPPEPVTGDPDDDL
                     ILACAVESSATVLVSGDRRHLLPVDRHRGVRILTPQALLAELRS"
     gene            complement(1347..1577)
                     /locus_tag="PLJ64_09160"
     CDS             complement(1347..1577)
                     /locus_tag="PLJ64_09160"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HNC93690.1"
                     /translation="MSRLLSVSLPDELADEIESLVEASGKNKSELVRDALRRQIRLER
                     VYSVRRLGQSRAETAGIGPEDVEDLIDRFRKE"
     gene            1679..2587
                     /locus_tag="PLJ64_09165"
     CDS             1679..2587
                     /locus_tag="PLJ64_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93691.1"
                     /translation="MNQVIRRTAVLLLSLGLLGAGALGLASTANAARAIDSAPMASDG
                     VRYFAYLTPKGVPAVLDTWTEKTNLIRGAKDCQPLDIGGGRVLLTCGKADKDHMRITR
                     TASARGGMARKLPHNKSGFWFSIGKYWVEGNVPCTGTGGCLGGRAYVNWRTGAIDSPG
                     GIPDDFDRANLDRVKLRYQSPPFVPAGFENSWKVCWNGDVFVTDNGRIRVWKNRHSSM
                     VIGKGGVLKYECDNYQGLGVGSNRVTWNRGPVIHEYNVASGMSYRRRLKKPTRIVPVR
                     DGAVIAMKNRDGRVHDHYRVRFIALP"
     gene            complement(2584..3900)
                     /locus_tag="PLJ64_09170"
     CDS             complement(2584..3900)
                     /locus_tag="PLJ64_09170"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNC93692.1"
                     /translation="MALRSNQNLPPGPRLPSPMQTFGWWARPTGFLDRARRKYGKSFT
                     VRLAAQDPFIVVSDPDEIKQVFTAPPDVLHPGEGTRILEPIVGPNSILLLDEGAHMTQ
                     RKLVLPAFHGKRMAGLEPLVADVTREQIAAWPRGASAPIHPRMQRLTLEVILAAVFGV
                     RTGERLDPLRQSLTEMLDFGMNPLSLLPPARRAPFGRGPWAKFLKVRARTDELIYGEI
                     QDRRDRGDEGGSEIINSLIGAHHEDGSPMTDEEIHDELMTLLVAGHETTATQLAWTIE
                     RLTREPRVLETLVEAVDNGDGDPYLTAVIRESLRLRPVLLNAAPRTVKKPIEVGGRTY
                     VPRVHLLASAWLVQHDADIYPNPYAFQPERFLESDPGTYTWIPFGGGRRRCVGATFAQ
                     LEMAIVLRELLRTTEIRAGVDGPEATRRRAITVTPGRGAETILTGR"
     gene            complement(3935..5638)
                     /locus_tag="PLJ64_09175"
     CDS             complement(3935..5638)
                     /locus_tag="PLJ64_09175"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HNC93693.1"
                     /translation="MGKKGIAAGIASLMLLMAAPGAAPAAGPADGAGASARQQSMRQL
                     VVIGLAGRGKPMAAASQIAWPGSSRYRQFLTQAEYRQQFGASPATVRKIKSLRKRHGV
                     RRVILNPTRTVAIVIMDPPAARRLFCASRNLPGKPCRPAGFRSAIRQVSVGEVYPNGP
                     TPRPPKASSSGSGTPHGCNGALKSKALTPNQISTAYEVDALRQRGLDGSGIRVATLSG
                     NVVGTADFRTWAKCFGLPAPKVSQPGMPSGVYDTQSDPDETVLDVEALSFLAPKLDRV
                     LPIFVPQDNKFNNPLLLFLFGALDPARLGGKLPDILSISDGVCEYQFRKAEKWLAQRL
                     LAEAAAIGITTLAASGDAGFLGCDMQKPGAQFPASSRFVTAVGGTNLGLDAGNSILSQ
                     PVWSTYAKKPSQGAGTGGGPSRFWPRPAFQVAPGLTPALQKGRKTRLLPDLAAMASFS
                     PGIATFEKGTGGWGPGGGTSAATPLTAAIVALVLQQEKEAGRPPLGLITPLLYKLSQG
                     GDYSQYFRDVTVGTSSRKPKTAAGKTPAGGAAQPGYDLATGLGSLRATALADAVAAMA
                     P"
     gene            5703..>6680
                     /locus_tag="PLJ64_09180"
     CDS             5703..>6680
                     /locus_tag="PLJ64_09180"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC93694.1"
                     /translation="MSESSPSPPIRVSIAVALLALSGAWNAGNVGPVASQLAADFDVS
                     LAMVGLVGGTLFFAANVIGLAFAAQIGEKIGLVWGMRTACLMIAAGNLVVAFTPDFAG
                     VALGRILPGLGFALTTTVGVVWARHEGGVKLVGIFGAAIQLGIAGSLLVGSLLYDQDV
                     DWRVGFVISASLAALAMAFIPPYAKSPPTPERKSSGFLRLAVRRARVYRLSLLFLSIY
                     GVPMILGAWMIEYLSSEGDVSKTIAGLASFLLFGLSAVMRFGGAELRAMGWTHKVLCG
                     SLGLAAAGLAAIAIDPVAGAAFLAAVLLATGFGLPYATALDEAQDLYPEE"
BASE COUNT         1033 a         2154 c         2331 g         1162 t
ORIGIN      
        1 gctcgagcag gatgtcggtt atcaggtaac cgctgagggt gaagaacacc ccgacgccga
       61 gcagtccgcc cgaggcccag tcaatcttga ggtggtaggc gatgaccgcc agcacggcca
      121 cgccgcgcag cccgtccagc cccggcatgt accgctgaga actctgtcca atcggtttcg
      181 gcatctacgg cgttggttcc tgcggttgcg ggtgcccgga accggtaact ctagttcgcg
      241 gacggggcgg gagatctgca caatcaccgc cgcggccgga acccttaggc tgccagcggg
      301 agacgatcat gaggcgtatc gaccgggaga tccagtcatg gagccgggac tctgcgcgag
      361 tccagcttgc cctgctctgg gacgaagtcg aacgggacgg cgtcaggtcg atctcctggt
      421 gggacggaga cccgtgggct tccgcgtatc tgatctcctc gacccagccc tggagtggcg
      481 gcatcgggaa gtggagggcc ttctgcatga tcaacttcgg ccgtcccgag gatgacgaga
      541 tcctcttctc ggatgatcgt gaagtggtgg gaaggaccag tcccgcccac ggaatgggtg
      601 cccgcgagat tcgccggatg ctcgagctgc ccggcgtgga ggaacggaag gaactctgcg
      661 acctgctgat cgccctgggt catcgccggg acgagcttga catcccgacg atcaccgggt
      721 tcgccgggca cgaagaccgg gtggtccgct acgtcgtgct cgcgatgctg tcctggaacc
      781 cttcgatcgc caacgactat ctggcgggca agttcagtga cgatcccgat ccgctcgtcc
      841 gggaacttgc ccggcgctgg tccggtggca ggttgcgccg ccggacccgc gcctgacccg
      901 gaacccgagc ctgacccgga agcgcgggtc aggacctgag ttctgccagc aaggcctgcg
      961 gtgtgaggat ccggactccc cggtgccgat ccaccggcaa caggtgccgc cggtccccgg
     1021 agacgagcac ggtggccgac gattcgacgg cgcaggcgag gatcaggtca tcgtccgggt
     1081 caccggtgac cggctccgga ggcccggctg gcggtccctg tgcatccacc gagatgtcag
     1141 cgagcagatc gagtaactgg cgcagttcag cggtatcgaa gtcgaacttt gccgggagga
     1201 ttcgtgtcaa ttcgttcagg accggttcaa gcaggacaag cccgaggctt ccgtcagctg
     1261 cttcctcgat cacccggccg ggcgggccgc ccgcgatgaa ggccgaaacc atcacgttgg
     1321 tgtcacaggc gatccttgtt gaagaatcac tctttgcgga accggtcaat cagatcctcg
     1381 acatcctcgg gtccgattcc ggccgtctcg gcgcggcttt gcccgaggcg ccggaccgag
     1441 tagacccgtt ccagcctgat ctgtcgccgg agggcgtcgc gaacaagttc ggacttgttc
     1501 ttgccggaag cttcgaccag cgattcgatc tcgtcggcaa gctcatcggg gagtgaaact
     1561 gaaagcagac gactcatatg aaatcatcat atcaacgggt tcatgagctt ccgggccccg
     1621 gggttgacgc cggcagtagg gtgttgccgt caactacgac ccgggaggca catcgtccat
     1681 gaatcaggtc attcgcagaa ccgcagtcct tcttctttcg cttggtctgc tgggtgccgg
     1741 cgcactggga ctggcttcca ccgcgaatgc ggcccgggcg atcgattcgg ccccgatggc
     1801 cagtgacggg gtgcgctact tcgcctacct gaccccgaag ggcgtcccgg cggttctgga
     1861 cacctggacc gaaaagacca acctgatccg gggagcgaag gactgccagc cgcttgacat
     1921 cggcggcggc cgggtgctgc tgacctgcgg caaggcggac aaggaccaca tgcggatcac
     1981 caggaccgcc tcggcccgcg gcggcatggc aaggaagctc cctcataaca agagcggctt
     2041 ctggttctcg atcggcaagt actgggtgga gggaaacgtg ccctgcaccg ggaccggtgg
     2101 ctgcctcggc ggtcgtgcct acgtgaactg gcggaccgga gcgatcgact cgcccggtgg
     2161 catccccgat gacttcgacc gggccaacct ggaccgggtg aaactccgtt accagtcccc
     2221 gcctttcgtc ccggccggtt tcgaaaacag ctggaaagtc tgctggaacg gtgacgtgtt
     2281 cgtgaccgac aacggccgga tccgtgtctg gaagaaccgg cactcatcga tggtgatcgg
     2341 caagggcggc gtactcaagt acgaatgcga caactaccag ggcctggggg taggaagcaa
     2401 ccgggtcacc tggaaccgcg gcccggtgat tcacgaatac aacgtcgcca gcggcatgag
     2461 ttacaggcgg cggctgaaga agccgaccag gatcgtcccg gttcgagacg gtgccgtgat
     2521 cgccatgaag aaccgtgacg gcagggtcca cgaccactac cgcgtccgct tcatcgccct
     2581 gccctagcgg ccggtcagga tcgtttcggc tccgcggccc ggggtcacgg tgatcgcccg
     2641 gcggcgggtt gcttccggcc cgtcgacacc ggcccggatc tcggtggtgc ggagcagttc
     2701 gcgcagtacg atcgccattt ccagttgcgc gaacgtggct ccgacgcagc gccgacgacc
     2761 acccccgaag ggaatccagg tgtaggtgcc gggatctgac tcgaggaagc gttcgggctg
     2821 gaaggcgtag gggttcgggt agatgtcggc gtcgtgctgg accagccagg cgctggccag
     2881 caggtgaacc cgcggcacat aggtccggcc gccgacctcg atcggcttct tgaccgtgcg
     2941 gggggccgcg ttcagcagca ccggccggag cctcaggctc tcgcggatca ccgcggtcag
     3001 gtaggggtcg ccgtcaccgt tgtcgaccgc ctcgaccagc gtttccagga cccggggctc
     3061 gcgggtcagt ctttcgatcg tccaggcgag ctgggtcgcg gtcgtttcgt gacccgccac
     3121 cagcagggtc atcagctcgt cgtgaatctc ctcgtcggtc atcggcgaac cgtcttcgtg
     3181 gtgggcgccg atcagcgagt tgatgatctc gctgccgccc tcgtcgccgc ggtcgcgccg
     3241 atcctggatc tcgccgtaga tcagctcgtc ggtcctggcc cggaccttga ggaacttcgc
     3301 ccagggaccc cggccgaacg gggcccgccg ggccggcggc agcaggctga gcgggttcat
     3361 gccgaaatcc agcatctcgg tcagtgactg gcgcagcggg tcgagtcgct caccggtccg
     3421 gactccgaac acggcggcga ggatcacctc gagggtgagc cgctgcatgc gcgggtggat
     3481 cggggccgat gcgccccgcg gccaggctgc gatctgctcc cgggtcacgt ccgcgacgag
     3541 cggttcgaga ccggccatgc gcttgccgtg aaaggccggc aggaccagct tgcgctgcgt
     3601 catgtgagca ccctcgtcga ggaggaggat cgagttcggg ccgacgatcg gttcaagaat
     3661 ccgggtgcct tctccggggt ggagaacgtc cgggggagcg gtgaagacct gcttgatctc
     3721 atccgggtcg gagaccacga tgaacggatc ctgggcggcc agccggaccg tgaagctctt
     3781 gccgtacttg cgcctcgccc tgtcgaggaa gccggttggc cgagcccacc agccgaacgt
     3841 ctgcatcggg ctcggcaggc ggggaccggg gggcaggttc tggttagacc tcagggccac
     3901 gaaagaaagc ttacgcctgc aggaccgggt gtggtcaagg tgccatcgcg gcgaccgcgt
     3961 cggccaaggc ggtggcccgc agcgagccaa ggccggtcgc gaggtcatag cccggctggg
     4021 cggcaccgcc ggccggggtc ttgccggccg cggtcttcgg tttgcgggaa ctggtgccga
     4081 ccgtaacgtc gcggaagtac tggctgtaat caccgccctg gctgagtttg tagagcagcg
     4141 gggtgatcaa cccgaggggc ggccgtccgg cttctttttc ctgctgcagg acgagcgcga
     4201 cgatcgctgc ggtgagtggc gttgcggcgc tggtaccgcc accgggtccc cagccgccgg
     4261 tgcccttctc gaaggtggcg atgccggggc tgaaagatgc cattgcggcg agatccggca
     4321 aaagccgggt ctttctgccc ttctgcaggg cgggggtgag gccgggcgcg acctggaagg
     4381 ccggccgggg ccagaagcgg ctgggtccgc cgcccgttcc ggcaccctgc gaaggcttct
     4441 tcgcgtaggt ggaccagacc ggctgggaga ggatcgagtt gccggcatcg agcccgagat
     4501 tcgtgccgcc gaccgcggtt acgaaccggg aactggccgg gaactgagcc ccgggcttct
     4561 gcatgtcgca gccaaggaag cccgcgtcac cagaggcggc gagggtggtg atgccgatcg
     4621 ccgccgcctc cgcgagcagg cgctgggcca gccacttctc ggccttgcgg aactggtatt
     4681 cgcagacccc gtcgctgatc gagaggatgt ccggaagctt gcctcccagc cgggccgggt
     4741 caagcgcacc gaaaaggaag agcagcagcg ggttgttgaa cttgttgtcc tgtggtacga
     4801 agatcggcag gacgcggtcc agtttcggag ccaggaagga aagcgcctcg acgtcgagca
     4861 cggtttcgtc cgggtcactc tgggtgtcgt acacgccgct cggcatgccc ggctggctca
     4921 ccttcggggc cggcaggccg aagcatttgg cccaggtccg gaaatccgca gtgccgacca
     4981 cgttgccgct cagggttgcc acccggatgc ccgagccgtc gaggccccgc tggcgcagtg
     5041 cgtcaacctc gtaggcggtc gagatctggt tcggggtgag ggccttcgac ttcagggcgc
     5101 cgttgcagcc gtgcggcgtg cccgagcccg acgaggaggc cttcgggggc cgaggagttg
     5161 gcccgttcgg gtagacctcg ccgaccgaga cctggcggat cgcgctcctg aacccggccg
     5221 gccggcaggg cttcccgggc aggttgcggc tggcgcagaa gagccgcctg gcggcgggcg
     5281 ggtccatgat cacgatcgcc accgtgcggg tcgggttgag gattacccgg cgcaccccgt
     5341 ggcgcttccg aaggctcttg atcttcctga ccgtcgcggg gctcgccccg aactgctggc
     5401 ggtactcagc ctgggtcagg aactggcggt agcggcttga gcccggccag gcgatctgtg
     5461 acgcggccgc catcggcttg ccccttccgg ccagcccgat caccacgagc tgtcgcatcg
     5521 actgctggcg ggccgatgct ccggcgccgt cggcgggccc cgcggcgggg gcggctcccg
     5581 gcgctgccat cagcagcatc agcgaagcaa tccctgcggc aatccccttc ttccccatgc
     5641 ggggagtatc gccgatggtc cctggctggt ccagcttccg gaactcggtg tagtttctga
     5701 tcgtgtccga gtcctcgcct tcgcctccga tcagggtttc gattgcggtt gcactgctgg
     5761 cgctctccgg agcctggaat gccggcaacg tcgggccggt cgcctcgcag ctggcggcgg
     5821 acttcgacgt ttcgctggcg atggtcggtc tggtcggtgg caccctgttc ttcgcggcca
     5881 acgtgatcgg gctcgccttc gccgcgcaga tcggggagaa gatcgggctg gtctggggca
     5941 tgcgaaccgc ctgcctgatg attgcggccg gcaacctggt cgtcgccttc actcccgatt
     6001 tcgcgggcgt cgccctgggc cggatcctgc cggggctggg cttcgccctg accacgacgg
     6061 tcggggtcgt ctgggcccgt cacgagggcg gggtcaagct ggtcgggatc ttcggggcgg
     6121 cgatccagct cggcattgcc ggttcccttc tggtcggcag cctgctctac gaccaggatg
     6181 tcgactggcg ggtcggcttc gtgatcagtg cctcgcttgc cgcgctggcg atggcgttca
     6241 tcccgcctta cgcgaagagc ccgccgaccc cggaaaggaa gtcctccggc tttctccggc
     6301 tggcggtcag gcgcgcccgg gtctaccggc tgtccctgct cttcctttcc atctacgggg
     6361 tgccgatgat cctcggcgcc tggatgatcg agtacctctc cagtgagggg gacgtttcca
     6421 agacgatcgc cggcctcgct tcatttctgc tgttcggcct ctcggcggtg atgcgcttcg
     6481 gcggggcgga gctgagggcg atgggctgga cccacaaggt gctttgcggc tcgctcggcc
     6541 tggccgcagc cggcctcgcc gcgatcgcga tcgatcccgt cgccggggcc gccttcctcg
     6601 ccgcggtgct gctcgcaacc gggttcggcc tgccctacgc caccgcgctc gacgaggccc
     6661 aggacctcta tcccgaggag
//
LOCUS       DAOQKN010000138         6653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_5778_length_6653_cov_5.327220, whole genome shotgun sequence.
ACCESSION   DAOQKN010000138 DAOQKN010000000
VERSION     DAOQKN010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 6653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6653
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5778_length_6653_cov_5.327220"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1544
                     /locus_tag="PLJ64_09185"
     CDS             <1..1544
                     /locus_tag="PLJ64_09185"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNC93695.1"
                     /translation="LEAIGEEFPAEAETVVGEVAFSSEWKWSALSFDEGGARYSLVLG
                     APDLMIGAGALTLDEPLAARLEEETGAGRRVVALCRTTSPLPASGLDGHPAGLEPIAL
                     VVLRENLRPGVEETLELMRSEDVALKLISGDAKATVTAVAAAVGIPTDAGVIEGPDLP
                     EDEEELARVVAENTIFCRIRPEQKRQLVSALAESGAYTAMIGDGVNDVPALKSARMAV
                     AMGSGSQVTKSVADVVLLKDQFSRLPEAIAEGRRIARNIHRLGRLYLTKTVYAAALII
                     LVSVPGFTFPFLPRHLTLAAFLTIGIPSFVLALAPSDGPLYRGRLLRALGAFAIPAGL
                     ATALSSILSFFLVDIAFGGSLEDGRTAATTTLIILGLCFVLLLERGPGREHIAIQSYM
                     LAMVAGLGALFAGILAVPQLREFFDMTMLGAEQWFIAMLSAALGLVFASLMWRLPQIQ
                     AWEVPDEDSFPPFERTPPPDNDRRPMPGPTHHRPRPEESPTEVIPAEADLLDEPPQNG
                     APTNH"
     gene            complement(1635..2144)
                     /locus_tag="PLJ64_09190"
     CDS             complement(1635..2144)
                     /locus_tag="PLJ64_09190"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HNC93696.1"
                     /translation="MTQVTAKDPGHETGSKSAHEAAFQSMRSAYLLLSERFEKELAEA
                     GLPDLGFYGVLLALSEAPEPSRPRDLLCKVNVTKSGLTRLVDRIEKAGLVERTYCPSD
                     RRGTFLVITEEGRETLARMQPVRERVFTEGFIDLVDEEDAETVARVMESVSQGIYESL
                     NTDGACDEA"
     gene            2262..2819
                     /locus_tag="PLJ64_09195"
     CDS             2262..2819
                     /locus_tag="PLJ64_09195"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNC93697.1"
                     /translation="MSTTEQAVRIETGTYAVDPVHTQVGFEVKHLGISTFRGSFGGFE
                     GNITVGDEGIVAIEGSIDVSTVAVPEEQLIGHLLSPDFFDAENHPKGTFVSTGVSLVD
                     AETYEVTGNLTLRGVTNPVTLTVEVEGAGIGMDGSSVLSLKANGEVNRNDYGISWGAA
                     LDNGAAVVAEKVRLVLTVEAVKAGE"
     gene            2822..3406
                     /locus_tag="PLJ64_09200"
     CDS             2822..3406
                     /locus_tag="PLJ64_09200"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNC93698.1"
                     /translation="MKVLAIVGSLRENSLSEQVARAAGDLAPEGVEVGIYEGLASVPA
                     YNADHDPALDQPEPAPAEVEDLRAQISAADALLIVTPEYNAGPPGYLKNAIDWASRPH
                     KASSLAGKPAAIVSQSPMPFGGIWANQALRKSFTITGTPTVERELAIGKSDEKLEDGR
                     LVDDQARADLTALMAELNKLVAVISDQEREAVAA"
     gene            3418..4632
                     /gene="lhgO"
                     /locus_tag="PLJ64_09205"
     CDS             3418..4632
                     /gene="lhgO"
                     /locus_tag="PLJ64_09205"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HNC93699.1"
                     /translation="MSRTDRPPSGCDIAVAGGGILGLAVARELAAREPDARICLLEAE
                     GRLAAHQSSHNSGVVHAGVYYAPGSLRAKLCVEGAGQLRDYCAERELPWRASGKLIVA
                     TGEAELPRLDELERRGQANGVKGLARVEPDEIPEIEPAARGRSALYSPFTAVTDFAAI
                     AGSFADDFEEAGGSIHLGSPVLGASSSGSRLRLRLPYGSLECGRAVFCAGLQSDRLAV
                     ACGGSPEPRIVPIRGGYLKVKPGPEDLVRGNVYPVPDPELPFLGAHLTRTFDGSLLIG
                     PTAMIAGARDAYDLTRLNRRDLGATLRWPGTWALMHRHWRASLNEAANSIRPARLARE
                     AARMVPSLSPADVVPGPAGVRAQALDRKGNLVEDFLIEETGSAIHVRNAPSPAATSSL
                     ALAGLIADRVFD"
     gene            4678..4956
                     /locus_tag="PLJ64_09210"
     CDS             4678..4956
                     /locus_tag="PLJ64_09210"
                     /inference="COORDINATES: protein motif:HMM:NF024845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HNC93700.1"
                     /translation="MTDALACPKCGSEMRSYERNQVVVDQCTGCGGLFLDRGELERLV
                     EAESSFYSKPQSSAPPQQEHRDDRKYDDRGKGKKKKSKRSFLEDFLEF"
     gene            complement(5103..6284)
                     /locus_tag="PLJ64_09215"
     CDS             complement(5103..6284)
                     /locus_tag="PLJ64_09215"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNC93701.1"
                     /translation="MPEAVIVDTVRTPIGRAFKGSLSQVRPDETGAFVIDQLLERNPG
                     VDPKAVEEVFCGVGMPQGKQAYNLARILVLLSDKLTQATTGVTVSRYCASSLDAIRHA
                     SNAVKNGEGDIYIAAGVEFVSQFNARTEIAGSIAAGEDDQNENLIGNNGQPNAYIDMG
                     LTAVNVANDYGVSREAMDDYALRSQHLAVASQESGFFDREIVPVTLPDGTVVSKDDGP
                     RADGGDMREKLASLPEAFGGGGVTAGNSCPLNDGAAAVLIMSDTKAAELGLTPRAKIV
                     TSATAGNEPEKMGVAPIWAVKKLLERANMSINDIDTVELNEAFAAQVIPISEEVGIPM
                     DKLNTHGGAIALGHPFGMTGARIMGTLLNVMETDDHQFGIETMCVAGGQGEAMLVERL
                     K"
BASE COUNT         1076 a         2263 c         2191 g         1123 t
ORIGIN      
        1 ccctcgaggc gatcggcgag gagttcccgg ccgaggccga aacggtggtc ggcgaggtgg
       61 ccttctcatc cgagtggaag tggagcgccc tgagcttcga cgaagggggt gcccgctaca
      121 gcctggtgct cggggcgccg gacctgatga tcggggccgg cgcgctgacc ctggatgaac
      181 cgctcgccgc ccgcctggaa gaagagaccg gtgccggccg tcgggtcgtg gccctctgcc
      241 ggacgacctc cccgctgccg gccagcggcc tcgacggaca cccggccggg ctcgagccga
      301 ttgccctggt ggtcctgcgc gagaacctca ggccgggggt cgaggaaacc ctggagctga
      361 tgcgaagcga agacgtggcc ctcaagctga tctcggggga cgccaaagcg acggtcaccg
      421 cggtcgccgc agcggtcggg atcccgaccg acgccggggt gatcgagggt ccggacctgc
      481 ccgaagacga ggaggagctg gcccgggtcg tcgcggaaaa cacgatcttc tgccggatcc
      541 gccccgaaca gaagcgccag ctggtttcgg cacttgccga gtccggcgcc tatacggcga
      601 tgattggtga cggcgtcaac gacgtgccgg ccctgaagag cgcccggatg gccgtcgcga
      661 tgggctccgg cagccaggtg accaagtcgg tggccgatgt ggtgctgctc aaggaccagt
      721 tcagccgcct gccggaagcg atcgccgagg gccgccggat cgcccgcaac attcaccgcc
      781 tcggccgcct ctacctgacc aagaccgtct acgcggcggc cctgatcatc ctggtctcgg
      841 tacccggctt cacctttccc ttcctgccgc ggcatctcac cctggccgcc ttcctgacga
      901 tcggcatccc ctcattcgtc ctcgccctcg cgccgtcgga cgggccgctc taccgcggca
      961 ggctgctcag ggcgctcggg gccttcgcga tcccggccgg cctggcgacc gcgctttcct
     1021 cgattctctc gttcttcctc gtcgacatcg ccttcggcgg cagcctcgag gatggacgca
     1081 ccgccgcgac gaccaccctg atcatcctcg gtctctgctt cgtgctgctt ctggagcgcg
     1141 gcccgggccg cgagcacatc gcgatccaga gctacatgct ggccatggtc gccgggctcg
     1201 gagcgctgtt cgccgggatc ctggccgtgc cccagctgcg cgagttcttt gacatgacga
     1261 tgctaggggc cgaacagtgg ttcatcgcca tgctttcggc cgcgctcggc ctggtctttg
     1321 ccagcctgat gtggcgcttg ccccagatcc aggcctggga ggttccggac gaggattcgt
     1381 tcccgccctt cgaacgaacc cctcccccgg ataacgaccg caggccaatg cccggtccga
     1441 cccaccaccg gccccggccc gaggaatctc ccaccgaagt gatccccgcc gaagccgacc
     1501 tgctggacga acccccacaa aacggagctc cgaccaacca ttagatattt ctagtgggcg
     1561 gagcgggttc cgattggagg tccggcgaag accggaccga ctgcagtcat tttcggaccc
     1621 cgcccgactc gcagtcaggc ctcgtcgcag gcgccgtcgg tgttcagtga ctcgtagatg
     1681 ccctgggaga cgctttccat cacccgggcc actgtctccg cgtcttcttc atcgaccagg
     1741 tcgatgaaac cttcggtgaa gacccgttcg cggacgggct gcatcctggc cagagtctcg
     1801 cggccctctt cggtgatgac gaggaaggtg ccgcggcgat ccgaggggca gtaggtgcgc
     1861 tcgaccagcc ccgccttctc gatccggtcg accaggcggg tcagtccgct cttggtcacg
     1921 ttgaccttgc agagcaggtc gcggggccgg ctcggctcgg gagcttcgct cagcgccagc
     1981 agcactccgt agaacccgag gtcgggcaga ccggcttcgg ccagttcctt ctcgaagcgc
     2041 tccgaaagca gcaggtaggc gctgcgcatt gactggaatg cggcttcgtg agccgatttc
     2101 gagccggtct cgtgccccgg gtcttttgcc gtcacctgtg tcatagagca cattgtagcg
     2161 caagtagttg acagcgcaac tatttttgct atggtgtccg tacaacagtt gcttacgcaa
     2221 caatttctca gtcaacttga attgaaacga agggaaacaa aatgagcacc accgaacagg
     2281 cagtacgcat cgagaccggc acctacgccg tcgaccccgt ccacacccag gtcggcttcg
     2341 aggtgaagca cctcggcatc tccaccttcc gcggcagctt cggcggcttc gagggcaaca
     2401 tcaccgtcgg tgacgaggga atcgtcgcga tcgagggttc gatcgacgtc tccaccgtcg
     2461 ccgttcccga agagcagctg atcggccacc tgctcagccc cgacttcttc gacgcagaga
     2521 atcaccccaa gggcaccttc gtttcgaccg gcgtaagcct ggtcgacgcc gagacctatg
     2581 aggtcaccgg caacctgacc ctgcgcggcg tgaccaaccc ggtgacgctg accgtcgagg
     2641 tcgagggcgc cggcatcggc atggacggca gctccgtcct cagcctcaag gcgaacggtg
     2701 aagtcaaccg caacgattac gggatctcct ggggcgccgc gctcgacaac ggtgccgccg
     2761 tcgtcgccga gaaggtccgg ctcgtcctca cggtcgaggc agtcaaggcg ggagagtaac
     2821 catgaaggtc ctcgcaatcg tcggaagtct ccgcgagaac tcgctttcgg agcaggtcgc
     2881 ccgcgccgcg ggtgaccttg ccccggaggg cgtggaagtc gggatctatg aaggcctggc
     2941 ttcggtcccg gcctacaacg ccgatcacga tccggctctt gaccagcccg agccggctcc
     3001 ggcggaagtc gaggacctgc gggcacagat cagcgcggct gacgccctgc tgatcgtcac
     3061 ccccgagtac aacgcgggcc ctcccggcta cctcaagaac gcgatcgact gggcctcgcg
     3121 gcctcacaag gcctcgtccc tggccggcaa gccggcggcg atcgtctccc agagtccgat
     3181 gcccttcggc gggatctggg cgaaccaggc gctgcgcaag tcgttcacga tcaccggcac
     3241 cccgaccgtc gagcgcgagc ttgcgatcgg caaatccgac gagaagctcg aagacggccg
     3301 cctggtcgat gaccaggccc gggccgacct cacggccctg atggccgagc tgaacaaact
     3361 ggtcgccgtg atcagcgatc aggagcgaga agccgtagct gcctagataa tcccggcatg
     3421 agccggaccg accgcccgcc atccggatgc gacatcgcgg tggcgggcgg cggcattctc
     3481 ggcctcgccg ttgcccgcga actggccgcc cgcgagcctg acgcccggat ctgtctgctg
     3541 gaagcggagg gccggctggc cgcccaccag tcctcccaca actcgggcgt ggtccacgcc
     3601 ggggtctact acgcgcccgg gtcgctcagg gcgaaactct gcgtcgaggg cgccggccaa
     3661 ctccgcgact actgcgccga gcgggaactc ccctggcggg cgagcggcaa gctgatcgtc
     3721 gcgaccgggg aggccgagct gccccgcctg gatgaactcg agcgacgggg acaagccaac
     3781 ggtgtcaagg ggctggcgag ggtggaaccg gacgagatcc cggagatcga gccggccgcc
     3841 cgcggccgtt cggccctgta ctcgcccttc accgccgtta ccgacttcgc ggcgatcgcc
     3901 ggatcattcg ccgatgattt cgaggaggct ggcggcagca ttcatctggg cagcccggtg
     3961 ctcggtgcct cttcgtccgg ctcccgtctt cggcttcggc tgccctacgg ctccctcgag
     4021 tgcggccggg cagtcttctg cgccggactt cagtccgatc gactggccgt cgcctgtggg
     4081 ggatcgcccg agccgaggat cgtgccgatc cgcggcggct atctgaaagt caagcccggc
     4141 ccggaggatc tggtccgggg caacgtctac ccggtgccgg acccggaact gcccttcctc
     4201 ggcgctcacc tgacccggac cttcgacggt tcgctgctga tcggcccgac cgcgatgatc
     4261 gccggggcac gggacgccta tgacctgacc cgtctcaatc gccgggacct gggagccacg
     4321 ctgcgctggc cgggcacatg ggccctgatg caccgtcact ggcgggccag cctgaacgag
     4381 gccgccaact cgatccggcc cgcccgcctg gcgcgcgaag cggcccggat ggtcccctcg
     4441 ctttccccgg ccgacgtggt tccgggacca gccggagtcc gcgcccaggc cctggaccgc
     4501 aagggaaacc tggtcgagga cttcctcatc gaggaaaccg gttccgcgat ccacgtccgc
     4561 aacgccccct ccccggcggc cacctcatcc ctcgccctgg ccgggctgat tgccgatcgc
     4621 gttttcgatt gaccgtccct ttaagaaagc tgaaagggcg ctggttaggt tcgcctcatg
     4681 actgatgctc tcgcctgccc caagtgtgga tctgaaatgc gctcctacga gcgaaaccag
     4741 gtcgtggtcg accagtgcac cggctgcgga ggactcttcc tcgaccgcgg tgaactcgaa
     4801 cgcctggtcg aagccgagag ttctttctac tcgaagcccc aatcctcggc gccgcctcag
     4861 caggagcacc gcgatgaccg caagtacgac gatcgcggca agggcaagaa gaaaaagtcg
     4921 aagcgctcct tcctcgaaga cttcctcgag ttctaggcag gaaaacccgg caccagccaa
     4981 ggtgccgcac gttggggccc gtgatgggcc tgtttgtgcg gcacctcggg attgatccgg
     5041 taacgagaaa gggcccggca tctgccgggc cctttcgcat gcaacgggat gttgcggtgc
     5101 cgctacttga ggcgctcgac cagcatggcc tcgccctggc cgccggcgac gcacatggtc
     5161 tcgatgccga actggtggtc atcggtctcc atgacgttga gcagggtgcc catgatgcgg
     5221 gcaccggtca tgccgaaggg atgaccaagg gcgatcgcgc caccgtgggt gttgagcttg
     5281 tccatgggga tgccgacctc ttcggagatc gggatcacct gcgcggcgaa tgcctcgttc
     5341 agctcgacgg tgtcgatgtc gttgatcgac atgttggcgc gctcgaggag cttcttgacg
     5401 gcccagatcg gggcgacgcc catcttctcc ggctcattgc cggcggtggc cgaggtaacg
     5461 atcttcgcgc gcggggtcag accgagttcg gccgccttgg tgtcgctcat gatcagaacg
     5521 gcagcagcgc cgtcgttcag ggggcaggag ttgccggcgg tgacgccgcc gccgccgaag
     5581 gcctcgggca ggctggcgag cttctcgcgc atgtcgccgc cgtcggcgcg cgggccgtcg
     5641 tccttgctga ccacggtgcc gtccggcagg gtcaccggga cgatctcgcg atcgaagaag
     5701 ccggactcct gggaggcgac tgccagatgc tgcgagcgca gggcgtagtc atccatggcc
     5761 tcgcggctga cgccgtagtc gttggcgacg ttgaccgcgg tgaggcccat gtcgatgtag
     5821 gcgttgggct ggccgttgtt gccgatcagg ttctcgttct ggtcgtcctc accggcggcg
     5881 atcgaaccgg cgatctcggt gcgggcgttg aactggctga cgaactcgac gccggcggcg
     5941 atgtagatgt caccctcgcc gttcttgacc gcgttcgagg cgtgacggat cgcgtcgagg
     6001 ctcgaggcgc agtaacgcga gacggtgacg ccggtggtgg cctgggtcag cttgtccgag
     6061 agcaggacga ggatgcgggc gaggttgtag gcctgcttgc cctgcggcat gccgacgccg
     6121 cagaaaactt cttcgaccgc cttggggtcg acaccggggt tgcgttcgag gagctggtcg
     6181 atcacgaagg cgccggtctc gtcgggacgg acctgggaaa gcgagccctt gaaggcgcgt
     6241 ccgatcgggg tgcggactgt gtccacaatc actgcttcag gcatgcgaag tctttctcct
     6301 tgggtttgtg tagctgaggt gtgagggcac ctggccggga catgacggat aaatgccact
     6361 aagcagcgta atgaactgcg gaaatgcggg acgggtagat tgactggcga tgagcaattc
     6421 gccccaggat ggcggcactt cagatgtgcc cataaacgcc ccgcaaggac ctccgcaggc
     6481 gccggccggt ggtcgtccgg cacccgcttc agccgggtcc ggccgcccgc aacagccgcc
     6541 cgcttcaccg cagccaggcc ggccttcgcc ctctcagccc gcccccgctc ccggccaggc
     6601 cggatcgtcc cggggtcagg cggccgcccc gcccgggact cccggccaga cct
//
LOCUS       DAOQKN010000139         6640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_5794_length_6640_cov_5.684131, whole genome shotgun sequence.
ACCESSION   DAOQKN010000139 DAOQKN010000000
VERSION     DAOQKN010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 6640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6640
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5794_length_6640_cov_5.684131"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1736)
                     /gene="ileS"
                     /locus_tag="PLJ64_09220"
     CDS             complement(<1..1736)
                     /gene="ileS"
                     /locus_tag="PLJ64_09220"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNC93702.1"
                     /translation="MSSFRPVDPKQSFPEMEERINAAWKESRVFERQLEQREDAEVWS
                     FYEGPPTANGRPGSHHVLSRVFKDIYPRYRAMRGYRVPRKAGWDCHGLPVELEVEKQL
                     GISSKQEIEDYGIEKFNQLCRESVFTYVEEWNRLTDRIGFWIDLDDPYVTMDSEYIES
                     VWWSLKELYESDRLYEGHRVSPYCPRCGTALSSHEVAQGYRDVIDPSVYVRFPLTGRD
                     ASLLIWTTTPWTLPGNYAVAVDPAVTYAEVDFEGERLVLAEKLVEKVLGEGVAAERTF
                     KGEELLGQGYQAPFPGMAENSGVFRVISGDFVTTDDGTGMVHIAPAFGEDDYRAAIAN
                     GLYDPSGKGAGGTLFNPVGLDGRFDARVTGFEGEFVKDPEVTERLIGHLRENGRLLKA
                     SDYEHAYPHCWRCGTPLLYYAKASWYIKTAEAKGAMLAGNEKIGWHPEHIKDGRFGRW
                     LENNVDWALSRDRYWGTPLPIWECSDPACEESFCAGSVAELEEKSGGPLPEDLHRPYI
                     DEVSWACECGQGEMKRVVSVIDTWYDSGAMPFAQFHYPFEGKEEFEQRFPADYICEAI
                     DQTRGWFYTLLA"
     gene            complement(1756..2868)
                     /locus_tag="PLJ64_09225"
     CDS             complement(1756..2868)
                     /locus_tag="PLJ64_09225"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNC93703.1"
                     /translation="MSGGPVGEETACPVVRDATGAWVLRRHEDVLAAALDPQRFSSRV
                     SRHLNIPNGMDGEQHARYRKLVERYMSPERTDRLEGRFREIARELVDSLPRDRPLDAV
                     SGVGSRFAVRAQSAWLGWPADAEESLLEWMDENRAAARSSSLEMSKAVADRFDRIIRA
                     LVEARRDPTGCPDTDVTAELIAARIDGRPLTVEEIVSILRNWTAGDLGTIAACIGVVI
                     RRLTTDPALQVRWRDHRPEAADMNREIDELLRIDDPFTVSRRVTTCPVSFGDQAIPAG
                     ARIRLDWAEANRDPEAVGDPEAFRPVENAGHNLVYGIGPHVCPGRGLATLEIRVVVEE
                     LLARISLEPDPELEPVRAEAPQGGFESVPVRLRELA"
     gene            complement(2865..3566)
                     /locus_tag="PLJ64_09230"
     CDS             complement(2865..3566)
                     /locus_tag="PLJ64_09230"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HNC93704.1"
                     /translation="MRDRPWLVRGSAFTVVLVLIQAGLGGLTVEHNLHATLVAVHLGI
                     AMILLALLLCMYRAAHPGPQPEAGRASAGLRATAAIAAFLVLATIVVGGYVAGTEGEG
                     TADKPSGGAHTACGTDFPTCGDELLPYGRDSMVDAQLTHRVLMFAASVSVLAMLALAL
                     MRGIRGWPAITAGGLVLLQVLLGALNIWLGKHAGLIIGHLTLGTLVWAVVVWAGITYT
                     RFPGPERSGAPPRPA"
     gene            complement(3509..3859)
                     /locus_tag="PLJ64_09235"
     CDS             complement(3509..3859)
                     /locus_tag="PLJ64_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93705.1"
                     /translation="MASTELTSGARTLIPFRRLAAATAFATFALIVVGGIVRVSESGL
                     GCGPAGSGLEGWPLCTGRVVPSVGGATLVEFTHRVLAGLVAVLVVLLPGRLSATCATG
                     PGWSGALPSRSCWC"
     gene            3956..5677
                     /locus_tag="PLJ64_09240"
     CDS             3956..5677
                     /locus_tag="PLJ64_09240"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC93706.1"
                     /translation="MDSMELRGKVVLITGGARGIGFETARLAREQGASVALIDLDPEI
                     VSESALALGGDTIGQAADVTDLGAMEAAVAQTVERLGGIDAVIASAGITPPKTTARAI
                     DPAVWEKVVEVNLVGVYNTARAAVEPIISSRGHMVLIASVAAQANGALNTSYAASKAG
                     VESLGRSLRAELEPHGASAGVAYFGYVETDLISEVFGNELADSFRREIAPDFLTRTMP
                     VSQVSGALIEGIRSRAPRIIEPPAWRPILGMRGAVGPFSDRALERNPKVGEFIREFER
                     RDLGEAAAPSRLPVTSGDLYALGGKTVLVTGAARGIGFEIARQAHGRGASVALLDLDR
                     SEAEAAAEAIGPRAAGFGCDVTSKKDLDLAFEATRERFGRIDVVVANAGIGPAQAGPI
                     RTQGTEEWNRVYEVDMFGVWRTVRAGIEDVIENGGQFVLVSSSYAFMNGLMNSAYAAA
                     KAGVEAFGRGLRVELTPLGASATVAYFGWIDTELVAGAFADPLVQRIREDVTPDWMTR
                     KVPVSKAGKVVIDAIEKRNPRAIAPTEWKALFYARGLLVPAFDKRIDEDQAIAQIVPQ
                     AEERQRA"
     gene            complement(5741..>6640)
                     /locus_tag="PLJ64_09245"
     CDS             complement(5741..>6640)
                     /locus_tag="PLJ64_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93707.1"
                     /translation="AAHFGPRAGRRRHRDDGQYELEDPTFGLWLQWRRPGGSIVPMTV
                     VGEYAERRFDIQGNPITPTPVAVEPPDYYNLGSSLQPTWVRTVPILSPFTEYDGYRIY
                     VWGSDHDPFQDTDEFYAKLIEEGADAYNTPSPLTLAAYLCSAGIPQADGTPRCPEQEC
                     PEGQTGYAPDHCVDIPVDPMIEIKSLKLTPKQGQVKAGKKAVLTLQVSTTDGIGGGNT
                     PIRLRSSNRNVKVPASVKVYLVSGKTLTKKITVTATRKAKGKAAITATSGKFKANSVL
                     KVKSLCVKKRSKGGNLCGKTNHF"
BASE COUNT         1073 a         2184 c         2263 g         1120 t
ORIGIN      
        1 tcggccagca gcgtgtagaa ccagccgcgg gtctggtcga tcgcctcgca gatgtagtcg
       61 gccgggaacc gctgctcgaa ctcttccttg ccctcgaagg ggtagtggaa ctgggcgaag
      121 ggcatcgcgc ccgagtcgta ccaggtgtcg atcacgctga ccacgcgctt catctcgccc
      181 tggccgcatt cgcaggccca gctcacttcg tcgatgtagg ggcggtggag atcctccggc
      241 agcggccctc cggacttctc ctccagttcg gcgaccgagc cggcgcagaa ggactcttcg
      301 caggccgggt cagagcattc ccagatcggc agcggggtgc cccagtagcg gtcacgggaa
      361 agggcccagt cgacgttgtt ctcgagccag cggccgaagc ggccgtcctt gatgtgctcc
      421 gggtgccagc cgatcttttc gtttccggcc agcatcgcgc ccttggcctc ggcggtcttg
      481 atgtaccagc tggccttggc gtagtagagc agcggggtgc cgcagcgcca gcaatgcggg
      541 taggcgtgct cgtagtcact ggccttcagc aggcggccgt tctctcgcag gtgaccgata
      601 agtctttccg tgacttccgg gtccttgacg aactcgccct cgaatccggt cacccgggcg
      661 tcgaaccggc cgtcgaggcc gaccgggttg aacagggttc ctccggcgcc cttgcccgaa
      721 gggtcgtaga ggccgttggc gatcgccgcc cggtaatcgt cctcaccgaa ggcgggggcg
      781 atgtggacca ttcccgtgcc gtcgtcggtg gtgacgaaat cgccgctgat cacccggaag
      841 acgccggagt tctccgccat gccggggaag ggtgcttggt atccctggcc cagaagctcc
      901 tcgcccttga aggtccgctc cgccgccacc ccttcgccga ggaccttctc gaccagcttc
      961 tcggccagga ccagcctctc gccttcaaaa tcgacttcgg cataggtaac cgcggggtcg
     1021 accgcgaccg cgtagttgcc gggcagggtc cagggggtgg tggtccagat caggagcgat
     1081 gcgtcccggc cggtcagcgg gaacctcacg tagaccgagg gatcgatcac gtccctgtaa
     1141 ccctgcgcca cctcatgcga agagagagcg gtgccgcagc gcgggcagta gggcgagacc
     1201 cggtgacctt cgtagagccg gtccgattcg tagagctcct tcagcgacca ccagaccgac
     1261 tcgatgtatt cggagtccat ggtcacgtac gggtcgtcga ggtcgatcca gaaaccgatc
     1321 cggtcggtca gccggttcca ttcctcgacg taggtgaaga ccgactcgcg gcagagctgg
     1381 ttgaacttct cgatcccgta gtcctcgatc tcctgcttgg aggagatgcc gagctgtttc
     1441 tcgacctcga gttcgaccgg cagtccgtga cagtcccagc ccgccttgcg cggcacccgg
     1501 tagccccgca tcgcccggta gcgcgggtag atgtccttga agacccggct cagaacatgg
     1561 tgggagccgg gccgcccgtt cgcggtcggg ggcccctcgt agaaactcca gacctcggcg
     1621 tcttcccgtt gctcgagctg ccgctcgaag acccggcttt ccttccaggc agcgttgatc
     1681 ctctcctcca tctcgggaaa ggactgcttc ggatcaaccg ggcgaaaaga cgacatgggc
     1741 gggggagttt atggatcacg caagctcccg gagtcgaacc ggcaccgact cgaaaccgcc
     1801 ctgcggcgcc tcggcccgga ccggttccag ttccggatcc ggttcgaggc tgatcctggc
     1861 gagcagctcc tcgacaacga cccggatctc gagcgtggcc agcccgcggc cggggcagac
     1921 gtggggcccg atcccgtaga ccaggttgtg gccggcgttc tcgaccggac ggaaggcttc
     1981 gggatcaccg accgcctcgg ggtcacggtt ggcttcggcc cagtcgagtc gtatccgggc
     2041 cccggccggg attgcctggt cgccgaacga gaccgggcag gtggtgaccc gccggctgac
     2101 ggtgaaggga tcgtcgatac ggagcaactc gtcgatctcg cggttcatat cggccgcttc
     2161 gggacggtga tcgcgccagc ggacctgcag ggcgggatcc gtcgtgaggc ggcggatcac
     2221 caccccgatg caggcggcga tcgtgccgag gtcgccggcg gtccagttgc gaaggatgct
     2281 gacgatctcc tcgaccgtga gtggccggcc atcgatccgc gcggcgatca gctccgcggt
     2341 cacgtcggtg tcagggcagc cggtcggatc gcggcgggct tcgaccagcg cgcggatgat
     2401 ccggtcgaag cggtccgcca ccgccttgct catctcgagg ctggatgacc gggcggccgc
     2461 acggttctca tccatccatt cgagcagcga ctcttcggca tcggccggcc agccgagcca
     2521 ggcgctctgg gcccgaaccg cgaaacggct gccgaccccg gacaccgcgt cgagcggcct
     2581 gtccctgggc agcgagtcga ccagctcgcg cgcgatctcg cggaaccggc cctcgagccg
     2641 gtcggttcgc tccgggctca tgtaccgctc gaccagcttc cggtagcggg cgtgctgctc
     2701 gccatccatg ccgttgggga tgttcaggtg gcgcgagacc cggctcgaga acctctgcgg
     2761 gtcgagggcg gcggcgagaa catcctcgtg tcgacgcaga acccaggcgc cggtcgcatc
     2821 acggacgacc gggcatgcgg tctcctcgcc gaccggcccg ccgctcaagc cggcctcggt
     2881 ggggcgccgg atctttccgg gccagggaac ctggtgtagg tgatgccggc ccagaccacg
     2941 accgcccaga ccagggtgcc aagcgtgagg tgtccgatga tcaggccggc gtgcttgccg
     3001 agccagatgt tcagcgctcc gagcaacacc tgcagcagaa ccagcccgcc cgcggtgatc
     3061 gccggccagc cccgaatgcc tcgcatcagg gccagggcca gcatcgcgag cacggacacg
     3121 ctcgccgcga acatcaggac ccggtgggtg agctgcgcgt caaccatgct gtcgcgcccg
     3181 tagggcagaa gctcgtctcc gcaggtcgga aagtcggtgc cgcaggcggt gtgggcgccg
     3241 ccggacggct tgtccgcggt cccttctcct tcggttccgg cgacatatcc gccgaccacg
     3301 atggtggcca ggaccaggaa tgctgcgatc gcggctgtag ccctgagccc ggcgctcgcc
     3361 cttccggctt ccggctgcgg ccccgggtga gccgcccggt acatgcagag cagcaaggcc
     3421 agcaggatca tcgcgatccc cagatgcacc gcgaccaggg tcgcgtgcag gttgtgctcg
     3481 acggtgagcc cgccgagacc ggcctggatc agcaccagca cgaccgtgaa ggcagagccc
     3541 cggaccagcc agggccggtc gcgcaggttg cggaaagcct gccaggcagg agcaccacca
     3601 gcaccgcgac caggccggcg agcacgcggt gggtgaactc gaccagggtc gcgcccccga
     3661 ccgaagggac caccctgccg gtacagagtg gccagccctc gagtccgctg ccagccggcc
     3721 cgcagcccag tcccgactcg ctcaccctga cgatgccacc gaccacgatc agcgcgaagg
     3781 tggcgaatgc ggtcgcggcc gcgagccgcc tgaaagggat cagggttctg gccccggatg
     3841 tcagctccgt gctggccatc ccgccgagga tagttcgcag aatctcctcg ggattgcggt
     3901 ttaaccacat ccagcctgcg gttttccacc ggcaaacagg ctctagagtg gtgtgatgga
     3961 ttcgatggag ctgaggggca aggtcgtgct gatcaccggt ggtgcccggg gaatcggttt
     4021 cgagacggcg cggctggccc gggagcaggg agcttccgtc gctttgatcg atctcgatcc
     4081 ggaaatcgtt tccgagtctg ctctcgcgct gggcggggac acgatcggcc aggccgccga
     4141 cgtgaccgat ctgggcgcga tggaagcagc cgtcgcccag accgttgaac ggctgggcgg
     4201 tatcgacgcg gtgatcgcga gcgccggcat cactccgccg aaaacgacgg cgcgggcgat
     4261 cgatccggcg gtctgggaga aggtcgtcga ggtcaacttg gtcggcgtgt acaacacggc
     4321 ccgggccgcg gtggagccga tcatctcgag ccgggggcac atggtgttga tcgcgtcagt
     4381 agccgcccag gcgaacgggg ccctcaacac ttcctacgcc gcctcgaagg ccggggtgga
     4441 atccctcggc cgttcactga gggccgaact cgaaccgcac ggagcttctg ccggggttgc
     4501 ctacttcggt tacgtggaga ccgacctgat cagcgaagtc ttcggcaatg agctcgccga
     4561 cagctttcga cgggagattg ctccggactt cctgacccgg acgatgccgg tcagccaggt
     4621 gtccggagcc ctgatcgagg gcatccgttc ccgggcgccg cggatcatcg aaccgcccgc
     4681 ctggcgtccg atcctcggca tgcggggagc ggtgggcccg ttcagcgacc gcgcccttga
     4741 gcggaacccg aaagtcggcg agttcatccg ggagttcgag cgccgggatc tcggcgaagc
     4801 cgccgcgccg tcgcggcttc cggtcacttc gggtgatctc tacgcgctgg gcggcaagac
     4861 ggtgctggtg accggggcgg cccgcgggat cggattcgaa atcgcccgcc aggcacatgg
     4921 ccgcggcgcg tcggtcgccc tgctcgacct cgaccgctcc gaagcggagg ccgcggccga
     4981 agcgatcggg cccagggcgg ccgggttcgg ctgcgacgtg acttcgaaga aagaccttga
     5041 cctggccttc gaagccaccc gtgagcgttt cggcaggatc gacgtggtgg tcgccaacgc
     5101 cgggatcggg ccggcccagg ccgggccaat ccggacccag ggcaccgagg aatggaaccg
     5161 ggtctacgag gtggacatgt tcggtgtgtg gcggaccgtg cgggccggga tcgaggacgt
     5221 gatcgaaaac ggcggccagt tcgtgctggt ctcgtcttcc tacgccttca tgaacggcct
     5281 gatgaactcg gcctatgccg ccgcgaaggc cggggttgaa gcgtttggcc gcgggctgag
     5341 ggtcgaactg acgccgctcg gagccagtgc caccgtcgcc tatttcggct ggatcgacac
     5401 cgagctcgtg gccggagcat tcgcggatcc gctggtccag aggatccggg aagacgtgac
     5461 cccggactgg atgacccgca aggtgccggt ttcaaaggcc ggcaaggtcg tgatcgacgc
     5521 gatcgaaaaa cgcaatccgc gagccatcgc cccgaccgag tggaaggccc tgttctacgc
     5581 ccgcggcctg ctcgtccccg ccttcgacaa gcgcatcgac gaggatcagg cgatcgccca
     5641 aatcgtcccc caggccgagg aacgccagcg cgcctgacaa ctcaccacag cgttgaagtt
     5701 atccgcacat ggaacgaata acgtcaacgc tgtctgattc tcaaaagtgg ttggtcttgc
     5761 cgcagaggtt gccgcccttg ctgcgcttct tcacgcagag ggacttgacc ttgagcaccg
     5821 agttggcctt gaatttgccg ctggtcgccg taatggcggc cttgcccttc gccttgcggg
     5881 ttgcggtcac ggtgatcttc ttcgtcaggg tcttgcccga aacgaggtag accttgaccg
     5941 aagcaggcac ctttacgttg cggttcgatg acctcaaccg gattggtgtg tttccgccac
     6001 cgatgccgtc ggttgtcgag acttgcagcg tcagcacggc cttcttgcct gccttcacct
     6061 gcccctgttt cggagtcagt ttcaggctct tgatctcgat catcggatcg acggggatat
     6121 ccacgcagtg gtccggggcg tagccggtct ggccttccgg gcattcctgc tccgggcagc
     6181 gaggggtgcc gtcggcctgc gggatgccgg ccgagcagag gtaggcggcg agagtcagcg
     6241 gcgagggcgt gttgtaggcg tcggcgccct cctcgatcaa cttggcgtag aactcgtccg
     6301 tgtcctggaa ggggtcatgg tcggaacccc agacgtagat cctgtagccg tcatattcgg
     6361 tgaaaggtga caggatcggc accgtgcgca cccatgtggg ttgaaggctt gacccgaggt
     6421 tgtaatagtc aggcggctcg accgcgaccg gagtcggggt gatcgggttg ccctggatgt
     6481 cgaagcggcg ctcggcgtac tcgccgacga cggtcatcgg cacgatcgag ccgcccggcc
     6541 gccgccactg cagccacagg ccgaaggtcg gatcctccag ctcgtactgc ccgtcgtcgc
     6601 ggtgccgacg acggccggca cggggtccga agtgggccgc
//
LOCUS       DAOQKN010000140         6536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_5943_length_6536_cov_6.029779, whole genome shotgun sequence.
ACCESSION   DAOQKN010000140 DAOQKN010000000
VERSION     DAOQKN010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 6536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6536
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5943_length_6536_cov_6.029779"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="PLJ64_09250"
     CDS             complement(<1..275)
                     /locus_tag="PLJ64_09250"
                     /inference="COORDINATES: protein motif:HMM:NF016349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HNC93708.1"
                     /translation="MNHLLRGHAPITDEAWKQIDEEARDGLVPALGGRRLVDFKGPLG
                     WGHSATDLGRVSDSTDGPADGVNLRTRQVLPVAELRAPFTISRSELEA"
     gene            complement(272..667)
                     /locus_tag="PLJ64_09255"
     CDS             complement(272..667)
                     /locus_tag="PLJ64_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93709.1"
                     /translation="MEFNQYHEPPEELSPELRTKARLFASLAEEAEAINWYEQRLSVE
                     PDAEARAIMKNAQHEEFKHFSMDLEFLLRKIPVWRELAEGVLFKDGDIVEHGEAAEAG
                     TDEEDEDATPEPAGSGGSLGIGSLRGAGL"
     gene            complement(757..1320)
                     /gene="pyrE"
                     /locus_tag="PLJ64_09260"
     CDS             complement(757..1320)
                     /gene="pyrE"
                     /locus_tag="PLJ64_09260"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNC93710.1"
                     /translation="MTDRDQTRQQLIGLLREHSLVLGEVTLSSGAKAGYYVDARRTVM
                     RPEGLKAAGTLIALHAKELGATAVGGPVMAAIPLACAAIAVPEGEGLGGFFVRKERKA
                     HGLQRWVEGAAEPGDRVLVVEDTVTTGGSTISAIERIRDEGFEIAGVLAVVDRLAGGG
                     EKIAAEAGAPYETLTTIDDIYPDRPDK"
     gene            complement(1346..2122)
                     /locus_tag="PLJ64_09265"
     CDS             complement(1346..2122)
                     /locus_tag="PLJ64_09265"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNC93711.1"
                     /translation="MEANQRAAEPAAGTDPVVFPPSPPGVDPPEDPGKYPFATWSPWV
                     AIGGAVAALIFGVMLALPVDVVAGGENFDDYGWQVKALFQLCTVVGFLAVPFLFAKEL
                     PGGVNWLEAAGRLGFHRFRIGTAAKWIGLGFLAYIAFAIVYAVVFGAPDQDDIAGELG
                     PLWSQILLIVILAPLSEEVCFRGMLFGGFRTRFNLPVAAIGAGLVFGALHYSTGITAV
                     PQLIVLGAIFAVVYEKSESIWPAIIFHLINNAYALAILNS"
     gene            2239..4365
                     /locus_tag="PLJ64_09270"
     CDS             2239..4365
                     /locus_tag="PLJ64_09270"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNC93712.1"
                     /translation="MTRRQRRVRRHRGSPGKVFVAVAAVLGGLLLTAIAAAGGWVLSI
                     AADAPNPDNLKEINKGQNSVIYAGDGSKLGLINSTEIRTPIPLSKIPQSIQDATVAIE
                     DERFYQHDGVDVQGGIRALFKNLEAGEVNEGASTITMQLMRNIYISNPQRDIERKIKE
                     AKMALDYEKDHTKKQILEKYLNTAPYGTNGGRSAIGVNAAAKVYFSVKPENMSLPQAA
                     LLAGLPQAPSDYNPISNPAGAKARRNEVLDAMADQNMITPARAAAAKQSGLQLNPADD
                     LFDREDPYFFDYVQSELQKKYGVNTVRRGGLKVYTTVQPSEQAAALSALDSNLYAGGP
                     SGALVAVDPRNGDIKAMVSSTNYEDSKFNLAAQGHRQPGSTFKVFTLTTAIHEGIDPY
                     TTYYESKPLYIDDPTYGHWEVHTYGDSYSGTISIHDATLASDNSVFAQLALDVGPDKI
                     ADMAHKMGITTHLDGYPAETLGGLTIGVSPLEMAGAYSTLASGGIRHDPTAIEKVVFP
                     NGEVDRPGRSKPVRVLSEAEAYEVTKILHDNITSGTGTGAYTGCGGQAGKTGTTDNQV
                     DAWFVGYQPNLTAAAWIGYPESNDLSTGSDGGDVPASIWNSFFYNAAVPCEETPIPTE
                     TMDWGDFSGGYTVSPGTQSDYGPQPEDTTGTDTGTGTGTDTGTGTVEPDGAYAPGVGQ
                     EPAGVGGGTGGTGGSTGGSTGGVTPG"
     gene            complement(4397..5482)
                     /locus_tag="PLJ64_09275"
     CDS             complement(4397..5482)
                     /locus_tag="PLJ64_09275"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HNC93713.1"
                     /translation="MSEESRTDPGEDEKPSLLHRLGHLAATVGKTGPKKKSTRIILQG
                     SIAFLIFAFLVFTVISQWSELKEKGVEFDFIWLIPAVVMMMLYYIAGAGVWGLILRFL
                     GSPVPAMETQKIWAQPLLVRYIPGTVLFLLARILMAEKAGVQRRVTTAGIVYEQAVSI
                     AGALTIATWFLIDHPDLQDQPARWLPLLVLPVMVCLLHPRVFGPVSKRLLHALGRESL
                     PQLMPFRGVVVIYLAYVVVWAIMGVGVFFAARAVHHLNLDDLAIVAASQMIGFLAAVV
                     SAVTPAGLGVRDAAFAWAVKAALPSDSFGVGAVIAIAVRATQTVVELIYVGGVTALTR
                     KEGDEDEIELAEEAAEEAARLQTESGS"
     gene            5507..6520
                     /gene="thiL"
                     /locus_tag="PLJ64_09280"
     CDS             5507..6520
                     /gene="thiL"
                     /locus_tag="PLJ64_09280"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HNC93714.1"
                     /translation="MDLSEFELIGKIADEARASDRFEIGIGDDAAVVRGRGRIVTSVD
                     TAVAGVHFDLDRPAAESARKAVASAISDLAAMGLGAGNTEILVALGAPPDTTDAYLEA
                     LAGGVAAAAEEFGADLAGGDVVASPVLFLSVTVIAHIDDETPVVGRKSAAGGEVLISG
                     PIGGAAAGLALLNGLEVSSLSPEDRAYLIDRQLRPNPRLDVAVAASNDGALAMIDISD
                     GLLADLGHVARASGFHIRLPAHRIPIDRGVADVARALGRDPLEFAVNGGEDYELAFIY
                     PGPILRHNYEIFHLPVPVYWIGQSVTPDHGGPLVGVHDGQGFLDLGHGGHDHFRGSDR
                     PSR"
BASE COUNT         1187 a         2207 c         2073 g         1069 t
ORIGIN      
        1 gcctcgagtt cagagcggct gatcgtgaac ggcgcccgaa gttccgccac cggcaggacc
       61 tggcgggtcc tcaggttgac tccgtcggcc gggccgtcgg tcgagtccga cacccggccc
      121 agatcggtcg ccgagtggcc ccagccaagg ggccccttga agtccaccag ccggcgacca
      181 ccgagcgccg gcaccaggcc gtcgcgcgct tcctcgtcga tctgcttcca ggcttcgtca
      241 gtaatcgggg cgtgaccccg cagaaggtga ttcaaagccc cgctcctctc aggctgccaa
      301 ttccaagcga tccacccgat ccggccggtt ccggggtcgc gtcttcgtct tcctcgtcgg
      361 ttccggcttc ggccgcctcg ccgtgctcga cgatgtcgcc gtccttgaac aggactcctt
      421 ccgccagctc ccgccagacc ggaatcttcc gcagcaggaa ctcgagatcc atcgagaagt
      481 gcttgaactc ctcgtgctgg gcgttcttca tgatcgccct ggcttcggcg tcgggctcga
      541 ccgagagccg ctgctcgtac cagttgatcg cctcggcctc ttcggccagg gaagcaaaca
      601 gtctcgcctt ggtcctgagt tcggggctca gttcctccgg tggctcgtgg tactggttga
      661 attccacgcc tggctccttt cgacggatct cgttgccggt gaactcatgc tctcactccg
      721 gggtccccgg gacaagcttc cggcaaaccg gacgccttac ttgtcggggc ggtccgggta
      781 aatgtcgtcg atcgtggtca gggtctcgta gggcgcgccc gcctcggcgg cgatcttctc
      841 gccgccgccg gccagccggt caaccacggc cagcacgccg gcgatctcga aaccctcgtc
      901 acggatcctt tcgatcgccg agatcgtcga accgccggtg gtcaccgtgt cctccaccac
      961 cagcacccgg tctccgggtt cggccgcgcc ttcgacccag cgctggagtc cgtgcgcctt
     1021 gcgttccttc cgcacgaaga aaccgcccag accctcgccc tccggcaccg cgatcgccgc
     1081 gcaggcgagc gggatcgccg ccatcaccgg accgccgacc gcggttgcgc cgagttcctt
     1141 cgcgtgcagg gcgatcaggg tgcccgccgc cttcagtcct tccggtcgca tcaccgtgcg
     1201 tcgggcgtcg acgtaataac ccgccttggc cccgctcgaa agagtgacct cgcccagcac
     1261 gagcgaatgc tcgcgaagca ggccgatcaa ctgctggcgg gtctggtcac ggtcggtcaa
     1321 gttcgttcct ctcggtcatc caccctcagg aattgaggat ggccagggcg taggcattgt
     1381 tgatcagatg gaagatgatc gccggccaga tcgactctga cttctcgtac acgacggcga
     1441 agatcgctcc cagcacgatc agctggggca ccgcggtgat tccggtcgag tagtgaaggg
     1501 cgccgaagac cagtccggcc ccgatcgccg cgaccggcag gttgaaccgg gtccggaaac
     1561 cgccgaagag catcccccgg aaacagacct cctcggaaag cggagccagg attacgatca
     1621 ggaggatctg ggaccagagc ggtccgagtt cgccggcgat gtcgtcctga tcgggagcgc
     1681 cgaaaaccac ggcatagacg atcgcgaagg cgatgtaggc gaggaatccg agcccgatcc
     1741 acttggccgc cgtgccgatg cgaaaccggt gaaacccgag ccggccagct gcttccagcc
     1801 agttgacccc gcccggaagt tccttcgcga agaggaaggg aaccgccagg aaaccgacca
     1861 ccgtgcagag ctggaacagc gccttgacct gccagccgta gtcatcgaaa ttctcgcccc
     1921 cggccacgac gtcgaccggc agggccagca tcacaccgaa gatcagggcc gcaaccgctc
     1981 cgccgatcgc gacccacggg ctccaggtcg cgaaggggta cttcccggga tcttcgggcg
     2041 ggtcgacccc gggtggggaa gggggaaaga caaccgggtc ggtcccggct gccggctccg
     2101 ctgcgcgctg gttggcttcc atggcttggc tcattctcac aattcggggc cgcagtcgtc
     2161 cgcaacccat cctgcaccgg cgtcgcctgg aaccgccctt tagaagttcc tgaggtgctt
     2221 ccagctaccc tttaggggat gaccaggcgc cagagaaggg tgcgccgtca ccgtggttca
     2281 cccggcaagg tttttgtcgc cgtggccgca gtgctcggcg gattgctgct aaccgcgatt
     2341 gcggctgccg gcggctgggt tctttcgatc gccgccgacg ccccgaatcc ggacaatctc
     2401 aaggagatca acaagggcca gaactcggtc atctacgccg gtgacggcag caagctcggt
     2461 ctgatcaact cgaccgaaat ccgcaccccg atcccgcttt cgaagatccc gcagtcgatc
     2521 caggacgcaa cggtcgcgat cgaggacgag cgcttctacc agcacgacgg cgttgacgtc
     2581 cagggcggca ttcgggccct gttcaagaac ctcgaagccg gtgaggtgaa cgaaggcgcc
     2641 tcgacgatca ccatgcagct gatgcggaac atctacatct cgaacccgca acgggacatc
     2701 gagcggaaga tcaaggaagc caagatggcc ctcgattacg agaaggacca caccaagaag
     2761 cagatcctcg agaagtacct gaacaccgcc ccctacggca ccaacggcgg acgcagcgcg
     2821 atcggcgtga acgcggccgc caaggtctac ttctcggtca agccggagaa catgagcctg
     2881 ccccaggccg ccctgctggc cggtctgccg caggccccct cggactacaa cccgatcagc
     2941 aatccggccg gggccaaggc ccgccgcaat gaagttctcg acgcgatggc cgaccagaac
     3001 atgatcacgc cggcccgtgc ggccgcggcc aagcaatccg gactccagct caacccggcc
     3061 gacgatctgt tcgaccgtga agatccgtat ttcttcgatt acgtccaaag cgaactgcag
     3121 aagaagtacg gcgtgaacac cgtccggcgc ggcggcctca aggtttacac gacggtccag
     3181 ccgtcggaac aggcggcagc cctttccgcc ctcgattcga acctctacgc gggcgggcct
     3241 tccggggcgc tggtcgcggt cgatccccgc aacggtgaca tcaaggcgat ggtttcctcg
     3301 accaattacg aggacagcaa gttcaatctc gccgcccagg gccatcgtca gcccggctcg
     3361 accttcaagg tcttcacgct gaccacggcg atccacgagg gcatcgaccc gtacacgacc
     3421 tactacgagt cgaagccgct ttacatcgac gacccaacct acggccactg ggaagtccac
     3481 acctacggcg attcctacag cggcacgatc agcatccacg acgccaccct ggcctcggac
     3541 aactcggtct tcgcccagct ggcccttgac gtggggccgg acaagatcgc cgacatggcg
     3601 cacaagatgg gcatcaccac gcacctcgac ggatacccgg ccgaaaccct cggcggtctg
     3661 accatcggcg tgtcaccctt ggaaatggcc ggtgcctact cgaccctggc cagcggcggc
     3721 atccgtcacg acccgaccgc gatcgagaag gtcgtcttcc cgaacggtga agtggaccgg
     3781 ccgggcaggt caaagccggt gcgggtcctc tccgaggccg aggcgtacga ggtcacgaag
     3841 attctgcacg acaacatcac cagcggcacc ggcaccgggg cctacaccgg ctgcggcggc
     3901 caggccggca agaccggcac caccgacaac caggtcgacg cctggttcgt cgggtaccag
     3961 ccgaacctga ccgcggccgc ctggatcggc tacccggagt cgaatgacct cagcaccggc
     4021 tccgatggcg gtgacgtgcc cgccagcatc tggaacagct tcttctacaa cgcggccgtg
     4081 ccctgcgaag aaaccccgat cccgaccgag accatggact ggggcgattt cagcggcggc
     4141 tacacggttt cacccggtac ccagagcgac tacggccccc agcccgaaga cacgacgggc
     4201 accgacaccg gaaccggcac gggcaccgat accggtaccg gcactgtcga gccggatggc
     4261 gcctacgcgc ccggggtcgg tcaggaaccg gccggcgtgg gtggcggcac cggcggtacc
     4321 ggtggcagca ccggcggttc aaccggcggc gtcacgcccg gttagccgga cggtcgcggg
     4381 ccggaaccct tcaggttcag ctgcccgatt cggtctgcag gcgggccgcc tcctcggcgg
     4441 cttcttcggc cagttcgatc tcgtcttcgt caccctcctt gcgcgtgagc gcggtgaccc
     4501 cgccgacgta gatcaactcg accaccgtct gggtggcccg gaccgcaatc gcgatgaccg
     4561 ctcccacccc gaagctgtcc gaaggcaggg cggccttgac cgcccaggcg aacgccgcgt
     4621 cacggacacc gaggccggcg ggagtgacgg ccgagaccac ggccgcgagg aacccgatca
     4681 tctgggaagc ggcgacgatg gcgagatcgt cgagattgag gtgatggacc gcccgggcgg
     4741 cgaagaagac gccgacgccc atgatcgccc agaccaccac ataggcgagg tagatgacga
     4801 cgacgccccg gaacggcatc agctgtggca gtgattcgcg gccgagcgcg tggagcaacc
     4861 gcttcgagac ggggccgaag acgcggggat gaagcaggca gaccatcacc ggcaggacca
     4921 gcaacgggag ccagcgggcc ggctgatcct gcaggtcggg gtggtcgatc agaaaccagg
     4981 tggcaatggt cagggcaccg gcgatcgaaa ccgcctgctc gtagacgatg ccggccgtgg
     5041 tcacccgtcg ctggaccccg gccttttcgg ccatgaggat ccgggccagc aggaacagga
     5101 cggtgccggg gatgtaacgg accagcagcg gctgggccca gatcttctgg gtctccatgg
     5161 ccggaaccgg gctgccgagg aaacgcagga tcaggcccca taccccggcc ccggcgatgt
     5221 aatagagcat catcatcacc accgccggga tcagccagat gaagtcgaac tcgacgccct
     5281 tctccttcag ctccgaccac tgtgagatga cggtgaagac caggaaggcg aaaatcagga
     5341 aggcgatcga gccctgcagg atgatccggg tcgacttctt cttcgggccg gtcttgccga
     5401 ccgtggcggc cagatgcccc agccggtgga gcaggctggg cttctcgtcc tcgcccgggt
     5461 ccgttctcga ttcctcactc atctgaggcg caatagtgtc agagcaatgg atctgagcga
     5521 gttcgaactg atcgggaaga tcgctgacga ggcccgggcc agcgaccggt tcgagatcgg
     5581 aatcggcgat gacgctgcgg ttgtccgggg ccgcgggcgg atcgtgacct cggtcgacac
     5641 agcggtcgcc ggggtccact tcgacctcga tcgccccgct gccgagagcg cccgcaaggc
     5701 cgttgcctct gcgatctcgg atctggcggc gatggggctc ggagccggca acacggaaat
     5761 tctggttgcc ctcggcgctc cgcccgatac cacggatgcc taccttgagg cgctggccgg
     5821 gggtgtcgcc gcggcggcgg aggaattcgg agccgacctg gccggcgggg atgtggtcgc
     5881 ctccccggtc ctcttcctca gcgttaccgt gatcgcccac atcgacgacg aaacgccggt
     5941 ggtcggccgg aagagtgcgg ctggagggga agtgttgatt tctggtccga tcggcggggc
     6001 cgcagccggg cttgccctcc ttaatgggct cgaagtatct tcgctttcac ctgaagatcg
     6061 cgcctatctg attgatcgcc agttgcgccc gaatccgaga ctggacgttg cggtggcggc
     6121 atcgaacgac ggtgccttgg ccatgattga catcagtgac ggacttctgg ctgacctcgg
     6181 tcacgtggca agggcgtcag gtttccacat tcggctgccc gctcaccgca ttccgataga
     6241 ccggggcgtc gcagatgtgg cgcgggcttt ggggcgggac ccccttgaat ttgctgtgaa
     6301 cggtggcgag gattatgaac tggcgtttat ctatcccggg ccgatcctgc gccacaacta
     6361 cgagattttt catctgcctg ttccggttta ttggatcggt caatcggtaa cgccggatca
     6421 tggtggcccc ttggtcgggg tgcatgacgg tcaggggttt ctcgatttag gtcatggtgg
     6481 ccacgaccac ttccgcgggt cggatcgccc ttcccggtag gctccggcgc catgcc
//
LOCUS       DAOQKN010000141         6500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_6007_length_6500_cov_5.360124, whole genome shotgun sequence.
ACCESSION   DAOQKN010000141 DAOQKN010000000
VERSION     DAOQKN010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 6500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6500
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6007_length_6500_cov_5.360124"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..1766)
                     /locus_tag="PLJ64_09285"
     CDS             complement(132..1766)
                     /locus_tag="PLJ64_09285"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNC93715.1"
                     /translation="MIRLTTKLSLVVTALVAGFLLPGPAGAAGTGSISGTVIDSDEQP
                     VTEGCVRAYDLADTRVSFSWIGPDGSYRLEGLDTGDYKVEFERCDANVLWEYYDNART
                     SEAATPVSVTDGVDTSGINARLDVGGTISGFVTFEPGFPAPGICVYADKPGAAGYWSG
                     SARVSPDGHYRIKGLETGDYKLEFRGCGDSDNVARQFYPGKATYSEAVPVSVTQGSET
                     PGIDADLAPGGAVTGTVFPAESPAEVCAMSVKAYDSDGNLAGSFNSPGFLYTPVDYRI
                     DQLITGNYRLHFEQGCLAIFSMDPFWVSEYFNNRNSLVEATPVSVTEGSTTSQISAVL
                     GSESTISGTITDTEGAPLENICVEAANEDGPAGIIAYSDPDGHYSLNALLGGSYTLKF
                     SDCQGTNERIVTPEYFDDKATIEEAVPVSVSAGGLRSGINARLATAPRPVPTAKISKL
                     KVKGPAFVRKGREATFRLRITNSGDATATGVRIKGSGRGISARAPVGKIAASTTRTVR
                     VRLRPSKAGKTMVTFRVTSSNAGGRTTKHKINVRKK"
     gene            1826..2956
                     /gene="cax"
                     /locus_tag="PLJ64_09290"
     CDS             1826..2956
                     /gene="cax"
                     /locus_tag="PLJ64_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HNC93716.1"
                     /translation="MSEPTTATGKRRMPEWFIWPYWLVPFIPVAIILDFFVGASATLI
                     FVTAALGVIPTAALMGVATEELAAKSGPGLGGLLNVTFGNAPELIIALFALGAGLQEV
                     VKASIVGAVLGNILLIMGIGMLAGGWKREKQTFNQTAVSSQALMLFLAVATLVLPAVF
                     VLVHGGGLPHVEEEITDFGSDVNTISIVASAILLFTYAAGLLFSLRTHKSFFNPSGEE
                     HGSPLGWSVRKAVGMLAFAGLAVGVMAHILVGSLEEVSVSLGLSEFFVGIIIVAIVGN
                     AAESWVSILYATKNKMDLSVQIAIGSATQLALVVMPTLVIVSFFLGPNPMALVFNGYE
                     MASLVIAVMAAYYVVQEGESNWFEGLQLLALYTVLGVVFYFA"
     gene            complement(2958..3293)
                     /locus_tag="PLJ64_09295"
     CDS             complement(2958..3293)
                     /locus_tag="PLJ64_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HNC93717.1"
                     /translation="MSFGSVYPHYVAKAERKGRSKDEVDEIIRWLTGYDQEQLESQIE
                     KGVDIETFYGEAPELNPSRELITGVVCGVRVEEIEEPLMQEIRYTDKLIDELARGKSM
                     GKILRAPAG"
     gene            3403..4125
                     /locus_tag="PLJ64_09300"
     CDS             3403..4125
                     /locus_tag="PLJ64_09300"
                     /inference="COORDINATES: protein motif:HMM:NF016143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HNC93718.1"
                     /translation="MRGLLATLLVSVVALTALSGCGTANSSNEPELPALPPIQHDLGP
                     VAYRELIGDTFELLNEYWNEQANRLGETMPPPRALVSFWTRAQDKGCGGEKLGWQNAQ
                     YCWQNRTIAWDGNWVYGVLYRRLGDAAVAFLLGHEYGHMVQDELGVSSEFRHTIEAEL
                     NADCLSGAWLGAVNDQVITFDRSDYESLYDGMLEVADAYGVPWTDPSAHGTAAQRGRA
                     LLFGARHGARACINQLGPGFTA"
     gene            complement(4129..4704)
                     /locus_tag="PLJ64_09305"
     CDS             complement(4129..4704)
                     /locus_tag="PLJ64_09305"
                     /inference="COORDINATES: protein motif:HMM:NF018984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HNC93719.1"
                     /translation="MDTAFLEELIEHKVAPEPLIAIQGLLNTHDYERDEELIGDAASA
                     RVWLAGADLIEEEATVSDEEVRDLTELRKILRRILESRTKGDEVDPGLYRDLQLLANK
                     YPAGYDVSDRGEVTLCLKPVETAGELIGQLVGIVGIAQENDWWERLKICADDDCRWAF
                     YDASKNRGGTWCRMEVCGNRNKNRRYRSKTN"
     gene            4807..4968
                     /locus_tag="PLJ64_09310"
     CDS             4807..4968
                     /locus_tag="PLJ64_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93720.1"
                     /translation="MFPFATLFARRSRPRTDLRTSVLDEEYEWVFGRKPSNELRQHRE
                     RPGERQDAE"
     gene            complement(4987..5334)
                     /locus_tag="PLJ64_09315"
     CDS             complement(4987..5334)
                     /locus_tag="PLJ64_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93721.1"
                     /translation="MALLLGASGAAASSTADAVVPREGHYSGHDHEWNPIGLNYSRTF
                     QVNHFSINHRARLGGYQVSGARWHGPCHHGLCTSGQWVNPNKIQGHWWHENSPAHKVG
                     YVVYWVNGMSGPG"
     gene            complement(5491..5859)
                     /locus_tag="PLJ64_09320"
     CDS             complement(5491..5859)
                     /locus_tag="PLJ64_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93722.1"
                     /translation="MIKRAFAVLAAALVLGVAGGATASTASATIIPREGHYSGHDHNH
                     NHIGLNYTVHGQMNHFTIDYSFVIGGAHVSNAMWHPTCHNGYCTSGAWVNKTTIQGHY
                     WHGGNPHHKVPFIVHWIHSR"
     gene            complement(5953..6135)
                     /locus_tag="PLJ64_09325"
     CDS             complement(5953..6135)
                     /locus_tag="PLJ64_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93723.1"
                     /translation="MADSSEDIQREFEEFEQAETSLDSGLESAPGRDRMPSWIKWIVI
                     ADLVIVIVVALIVFAA"
     gene            complement(6154..>6500)
                     /locus_tag="PLJ64_09330"
     CDS             complement(6154..>6500)
                     /locus_tag="PLJ64_09330"
                     /inference="COORDINATES: protein motif:HMM:NF025590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4333 domain-containing protein"
                     /protein_id="HNC93724.1"
                     /translation="SPRRVLVLAAVLASAFALSACEKEVSIGDNTIDRDELELESSRT
                     LTKQYGEKPASIDCPDDLKAEVDATETCELVDQSGNHYDMKVTVTSVDDDGNAKFNIK
                     VGGIKQMGSVPE"
BASE COUNT         1106 a         2089 c         1973 g         1332 t
ORIGIN      
        1 cttggccgaa ccggtcacga tcgcgtttcc gttgccggtt gcattggtgc cggtttcgga
       61 gtcgtcatcg ctgccgcacc cgaacagcat cgcggcacag gcaatcgtca aagttgctgc
      121 cagaacggat ttcacttttt cctcacgttg atcttgtgtt ttgtggtcct gccgccggcg
      181 ttcgatgaag tgacccggaa ggtgaccatc gtcttgccgg ccttgctcgg tctcagcctg
      241 accctgacgg ttctggtcgt cgacgcggcg atctttccga ccggcgccct ggcgcttatg
      301 ccgcgtccgc tgcccttgat ccggaccccg gtcgcggtgg cgtcaccgga gttggtgatc
      361 cgcaacctga atgtcgcttc ccggcccttc ctgacgaaag cgggaccctt caccttcagc
      421 ttgctgattt tcgccgtggg cactggtctc ggcgcggttg ccagccgggc gttgatgccc
      481 gatctcaacc cgccggcgga gaccgatacc ggcacggctt cctcaatcgt ggccttgtcg
      541 tcgaagtact cgggggtcac gatccgctca ttcgtgccct ggcagtcact gaacttcaat
      601 gtgtatgaac cgccgagcag cgcgttcaac gagtaatgac cgtccggatc gctgtaagcg
      661 atgatcccgg ccggtccatc ttcattggcc gcctcgacgc agatgttctc gagcggagca
      721 ccttcggtgt cggtgatcgt gcccgagatc gtgctttccg atcccagtac agcgctgatt
      781 tgggacgtgg tcgaaccttc ggtcaccgaa accggtgtgg cttcgacgag ggaattccgg
      841 ttgttgaagt actcggaaac ccagaacgga tccatcgaga agatcgcgag gcaaccctgt
      901 tcgaaatgga gcctgtagtt cccggttatg agctggtcaa tcctgtagtc aacgggcgtg
      961 taaaggaaac ccggggagtt gaacgagccg gcaagattcc cgtcggagtc gtaggccttg
     1021 accgacattg cgcaaacctc ggccggtgac tcggcgggaa acacggtgcc ggtgacagcg
     1081 cctcccggcg ccaggtcggc gtcaatcccc ggggtctcag acccctgggt taccgatacc
     1141 gggacggctt ccgagtaggt cgccttgccg ggatagaact gccgggccac gttgtcggag
     1201 tcaccgcatc cccggaattc gagcttgtaa tcgccggttt ccaaaccctt gatccggtag
     1261 tggccgtcag gggaaacgcg ggcggatccc gaccagtagc cggcggcgcc tggcttgtcc
     1321 gcgtacacgc agattcccgg ggcggggaaa cccggctcga aggtcacgaa acccgagatc
     1381 gtcccgccca cgtcgagccg ggcgttgatg cccgaagtgt caaccccgtc cgtgacggag
     1441 acgggagtgg cggcctcgga agttctggcg ttgtcgtagt actcccacag cacgttcgcg
     1501 tcgcaacgct cgaactcgac cttgtaatcg cccgtatcca gcccttccag cctgtaactg
     1561 ccgtccggcc cgatccagga gaaactgacc cgtgtgtccg ccaggtcata ggcccgcacg
     1621 caaccttcgg tcaccggctg ctcgtcgctg tcgatcaccg tgcccgagat ggaacccgtg
     1681 cccgccgcgc cagccgggcc cggaagcagg aaaccagcca cgagcgccgt gacgacgagc
     1741 gagagctttg tcgtaagtcg aatcattcaa ctcccctggt cagtgcgacc aaacgtcgcc
     1801 cgtcaggatc ataatctgct ttcacatgtc cgagccgacc acagccacag gcaagcgcag
     1861 gatgcccgaa tggttcatct ggccctactg gctggttccg ttcattccgg tcgcgatcat
     1921 cctcgacttc ttcgtcggcg cgagcgcgac cctgatcttc gtgactgcgg cgctgggtgt
     1981 gatcccgacc gccgccctga tgggtgtagc cacggaagag ctcgccgcga agtcggggcc
     2041 cggactcggc ggcctgctca acgtgacctt cggcaacgcc cccgaactga tcatcgccct
     2101 cttcgcgctc ggggcgggcc ttcaggaggt cgtaaaggcc tcgatcgtcg gtgcggtgct
     2161 cggcaacatc ctcctgatca tggggatcgg catgctggcc ggcggctgga agcgggagaa
     2221 gcagaccttc aaccagaccg ccgtctcatc ccaggccctg atgctctttc tcgcggtggc
     2281 aaccctggtt ctgccggcgg tcttcgtgct ggtccacggg ggcggcctcc cccacgtcga
     2341 ggaggagatc accgacttcg gctcggacgt caacacgatc tcgatcgtcg cttcggcgat
     2401 tctgctcttc acctacgccg ccgggctgct cttctcgctc aggacccaca aaagcttctt
     2461 caacccgtcc ggcgaagaac acggttcgcc gctcggctgg agcgtccgca aggcggtcgg
     2521 catgctcgcc ttcgccggtc tcgcggtcgg cgtgatggcc cacatcctgg tcgggtcgct
     2581 cgaggaggtt tcggtttcgc tcgggctgtc cgaattcttt gtcggcatca tcatcgtcgc
     2641 gatcgtcggc aacgccgcgg agagctgggt gtcgatcctc tacgcgacga agaacaagat
     2701 ggacctttcg gtccagatcg cgatcggttc ggcgacccag ctcgccctgg tcgtgatgcc
     2761 gaccctggtg atcgtctcct tctttctcgg cccgaacccg atggcccttg tcttcaacgg
     2821 ctacgagatg gccagcctgg tcatcgcggt catggccgcc tactacgtgg ttcaggaagg
     2881 cgaatcgaac tggttcgaag gcctccagct gctcgccctc tacaccgttc tcggcgtcgt
     2941 cttctacttc gcctgagcta cccggcggga gcgcggagaa tcttccccat cgacttgccc
     3001 cgggcgagtt cgtcaatcag cttgtcggtg tagcggatct cctgcatcag aggttcttcg
     3061 atttcctcga cccgtacccc gcagaccacc ccggtgatca gctcccgtga aggattgagc
     3121 tcgggtgcct ctccgtagaa cgtttcgatg tcgaccccct tctcgatctg cgactcgagt
     3181 tgctcctggt cgtaacccgt cagccagcgg atgatctcgt cgacttcgtc cttgctcctc
     3241 cccttcctct ccgccttcgc cacataatgc ggatagacgc tgccgaaact catcctgtaa
     3301 atgcggtgac cggtcaagac tctcctcgct ggtttcgagg atcctagcca cggagataca
     3361 aaatgatccg cgctcgggga gccatgtgcg acgatcggag agatgagggg gctcctcgcc
     3421 acactgctgg tttcggtggt tgccttgacc gctctgtcgg gctgcggtac cgcgaactcc
     3481 tcgaatgagc cggaactgcc cgccttgccg ccgatccagc atgacctcgg gccggttgcc
     3541 taccgtgagt tgatcggtga cactttcgaa ttgctcaacg agtactggaa cgagcaggca
     3601 aacaggcttg gcgagacgat gccgcctccg cgggcgctgg tttcgttctg gacccgggcc
     3661 caggacaagg gctgcggcgg cgagaagctc ggctggcaga acgcccagta ctgctggcag
     3721 aaccggacca tcgcctggga cggaaactgg gtctacgggg ttctgtaccg gcgcctcggc
     3781 gatgccgcgg tggcattcct gctcggccac gagtacgggc acatggtcca ggacgagctc
     3841 ggagtttcca gcgagttccg ccacacgatc gaggcggaac tgaatgccga ttgtttgtcc
     3901 ggggcctggc tcggtgcggt gaacgaccag gtgattacct tcgaccgttc cgactacgaa
     3961 tccctctacg acggaatgct cgaggtagcc gacgcctacg gggttccgtg gaccgacccc
     4021 tccgcccacg gcaccgccgc ccagcgtggc cgggccctgc tgttcggagc ccgccacgga
     4081 gcccgggcct gcatcaacca gctcggcccc ggcttcaccg cctgaacttc agttcgtctt
     4141 gctgcggtag cggcggttct tgttgcggtt gccgcagact tccatccggc accaggtccc
     4201 gccgcggttc tttgaggcgt cgtagaaggc ccaacggcaa tcgtcgtcgg cgcagatctt
     4261 caggcgctcc caccaatcgt tctcctgggc tatgccgact atgccgacca gctggccgat
     4321 cagctcaccc gcggtttcga ccggtttcag gcaaagggtg acttcacccc ggtcgctcac
     4381 gtcatagccg gccggatact tgttggcgag aagctgcaga tcgcggtaga gacccggatc
     4441 gacctcgtct cccttcgtcc gggactcgag gatgcgccgc aggatcttgc ggagttcggt
     4501 caggtcccga acttcctcgt cgctgaccgt cgcttcctcc tcgatcagat ccgcgccggc
     4561 cagccatacc cgtgcggatg cggcgtcacc gatcagttcc tcatcccgtt cgtagtcatg
     4621 cgtgttgagc agaccctgga tcgcgatcag cggttccggc gcgaccttgt gttcgatcag
     4681 ctcttcgagg aaggcggtgt ccatggatcc atcgtactcc tgcgtgaccg gtaaatcaac
     4741 ttgacaggtc actctaaccg gtgtaacgtt tttaccagtt agcaagaatt caactgaaag
     4801 gaagccatgt tccccttcgc gacactcttc gccaggcgca gcaggccgcg caccgacctc
     4861 cggacctcgg ttctcgacga ggaatacgaa tgggtcttcg gccgcaagcc ttcgaacgag
     4921 ctgcggcagc accgcgagcg gccgggcgag cggcaggacg ccgagtagcc cggacggact
     4981 gtcctgctat ccgggacctg acatcccgtt cacccagtag acgacgtagc cgaccttgtg
     5041 cgcgggtgag ttttcgtgcc accagtggcc ctggatcttg ttcgggttga cccactggcc
     5101 gctcgtgcag agcccgtggt gacaggggcc atgccagcgg gcgcctgata cctggtatcc
     5161 cccgagcctg gcccggtgat ttatcgagaa gtgattgacc tggaaggtgc ggctgtagtt
     5221 caggccgatc ggattccatt cgtgatcgtg acccgaataa tggccctcgc gcggtaccac
     5281 cgcgtcggcc gtgctcgatg ccgccgcacc ggacgcgcct aaaagcagcg ccaccgacag
     5341 gattgagatt gctcgtctga tcaagttgcc ctccctggtg atgcgaagcg gtagttcgct
     5401 ccgtcggaga ccaatatagg cgccccgtcc ggactggaca agaaaaaggc cggcggtctc
     5461 ccgccggcct ctccccgaat cacttccgtg ttagcgggag tggatccagt ggacgatgaa
     5521 cggaaccttg tgatgcgggt tgccgccatg ccagtagtga ccctggatgg tggtcttgtt
     5581 cacccaggca ccgctggtgc agtacccgtt gtggcaggtc ggatgccaca ttgcgttgct
     5641 cacatgcgcc ccaccgatca cgaagctgta atcgatcgtg aagtggttca tctgaccgtg
     5701 aaccgtgtag ttgaggccga tgtggttgtg gttgtggtcg tggcccgagt agtgcccctc
     5761 cctgggaatg atggtggcgc tggcggtcga tgccgttgcc cctccggcaa cgccgagcac
     5821 gagtgccgcg gcgaggacag cgaatgcgcg tttgatcaag gatccctctt tctgtagcgg
     5881 agcggacgaa ccccgcattg gtcggtaatc cgactataaa cgaacttccg aacgccgttc
     5941 ggtaaattcg tttcaggcgg cgaaaacgat cagcgcgacc acgatcacga tgaccaggtc
     6001 ggcgatgacg atccacttga tccacgaggg catccggtcc cggcccggag ccgactcgag
     6061 cccggaatca agcgaagtct cggcctgttc gaactcctcg aactcccgct ggatgtcttc
     6121 gctggaatca gccatggcgt tcgcggcccg gccctattcg ggaaccgaac ccatctgctt
     6181 gatgccgccg accttgatgt tgaatttggc gttgccgtcg tcgtcgaccg aggtgacggt
     6241 caccttcatg tcgtagtggt ttccactctg atcgaccagt tcgcaggtct cggtcgcgtc
     6301 aacctcggcc ttgaggtcgt cgggacaatc gatcgaagcg ggcttctccc cgtactgctt
     6361 ggtcagggtg cgtgaagact ccagctcgag ctcatcccgg tcgatcgtgt tgtcaccgat
     6421 cgaaacttcc ttctcacagg cgctgagggc aaaggccgac gcgagcactg cggccaggac
     6481 gaggacacgg cggggagact
//
LOCUS       DAOQKN010000142         6484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_6038_length_6484_cov_6.172500, whole genome shotgun sequence.
ACCESSION   DAOQKN010000142 DAOQKN010000000
VERSION     DAOQKN010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 6484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6484
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6038_length_6484_cov_6.172500"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="PLJ64_09335"
     CDS             <1..748
                     /locus_tag="PLJ64_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93725.1"
                     /translation="ADRFRGWGIPDPLINDFRSSSPDPHQPPPAHASRAIHPGLVIHR
                     TRSLKRGEVRNRHGIPVTSAVRTVINLAEREPPRSLDTAIRRGIARNLFDLDELNRAI
                     DRARGRKGIAKLRKVLANWNPGKLRTKSGLENKTADRFRGWGIPDPLINDFRCGHEVD
                     FQWPGSNILVESDGGAYHASRADRHRDYQKMLDLQAAGNVVVRIDEDMVEKEAYEVGL
                     KLRKLFAEEGVVELQPISPQPVAVSDRAPP"
     gene            complement(697..1131)
                     /locus_tag="PLJ64_09340"
     CDS             complement(697..1131)
                     /locus_tag="PLJ64_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNC93726.1"
                     /translation="MEMFQRDLTLEARSRGFHLVTDEIERGLPELGTLRIGLIHLLIR
                     HTSASLTLNENASPDVRRDFSSWIDSVVPERPTAYRWTHTLEGADDMPAHIKGSVTGF
                     DLTIPVRDGCFHLGAWQGIYLCEHRDHGGARSLTATGWGEIG"
     gene            complement(1352..1912)
                     /gene="efp"
                     /locus_tag="PLJ64_09345"
     CDS             complement(1352..1912)
                     /gene="efp"
                     /locus_tag="PLJ64_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNC93727.1"
                     /translation="MISTSDFRNGSHIEVEGKIMRIIEFQHVKPGKGGAFVRTKLRRI
                     EDGGVIDKTFRAGEKFRPVRTESKRMQYLYESGDAVVFMDNQSYDQIEIPADIAADAM
                     RWIQPNDEVEVLFVDENPSDVSVGSSIEAKVVKTEPGLKGDTASGGGTKPATLESGVE
                     VQVPLFINEGEVIKVNTETAEYMSRA"
     gene            1972..3036
                     /locus_tag="PLJ64_09350"
     CDS             1972..3036
                     /locus_tag="PLJ64_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HNC93728.1"
                     /translation="MTTTPALLDELLRTPGPSGYEAPAADVWRKAAGFAEITTDGLGS
                     SAATIPSSDENDEAPLVAVVGHIDEIGLVITHIDEKGFLYFAPIGGWDPQILVGQRVT
                     ITGNEGPVPGVAGRKPIHLLEPDQRKKVVQLKEMHIDIGADDREDAQKMVSVGDPVVI
                     DCEPQAIAGGRLVSRSMDNRLGSYVALEAARRIHENARAKVRFAGVAAVQEEIGLFGS
                     RTAAFTFRPDLAVAIDVTHATDAPGIDEKEVGSHPFGSGPVIGRGSTLSPKVYELLRD
                     AAEKAGIDYSVGASGNSTHTDADSLQISRTGIPTGLVSIPLRYMHSPVEMVDLADVEA
                     TVELMVAFVEGLAVDVDLSR"
     gene            3224..5929
                     /locus_tag="PLJ64_09355"
     CDS             3224..5929
                     /locus_tag="PLJ64_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93729.1"
                     /translation="MGITNARRFVALAFALVMAFALTAAFANSQAEAGKKKKKAKPAT
                     VKVVNITTANQQALLKQKKLTVKVKSTGKASPKVSAVSSGKSNYFKSVTVKFKKKGTK
                     TVSLALTSAGKTALGTCGAKTVQVNASYKKGKKKAKASKKKTLGKWADNSNCFVTVDV
                     GDDPAHCDFLDATQCLQPWPNDYYTKEDSSTPTGKRLNLGAESTPMNTGNATPVHLDV
                     TDINRGDGFSPGNEILIKIPGLDTPAAFTNSNLVPLDDISAYKDAAQAVIVIDAATGE
                     RQPIWAELDSNPTSVNPTEEDPGGININPTNTQPVNLIVHPAKNFEFGHRYIVAFRNL
                     KDADNNAIQSPLGFRVYRDNLKTKQSVVENRRSHMNSVINDLVNKAGVDRSSLYMAWD
                     FTVASEDSVTGRATTIRDDAFARLGDNNLADRQIAGNSPDIDVLAYCDKSNVAAPSCG
                     NNYPGIPEFNGSNPVTSPVPGTDIQRTVVGYIRDVPCYLDQDGCPASGSQFAFDSNGN
                     LTWNNTFKMDVPFRCMIPSSIVNTGTVVPGHTGTYGHGLLGSLNQINATVEAGNRTNT
                     VFCAANWDGFSTLDAGVVIQSLMNMSNFNKLVDRMQQGFVNFLYLQRALVHPDGFADQ
                     AAFQMDHNGATPITPGSAIDTSAGAATRGYYYGISQGGIMGGALTALSPDVDKGVLGV
                     PGINYSTLLSRSVDSDEYFQAPGYGLYPNYPDMSSRQLLLSLIQLLWDRGEGNGYANN
                     MTDNPLPNTNSHEVLLQLALGDHQVTNYAAEVEARTIGAVRYAPTLRPERTWDLDYEA
                     LPGNTSFPIGPNESFMVYYDGGPPSFNGTQDPGTAKPPLENVPPRTEWGFGEDPHGFP
                     RRALDANLHIKSFYENGTIDSCEATSPSPGDAYCYANGWDGVTGL"
     gene            complement(6308..>6484)
                     /locus_tag="PLJ64_09360"
     CDS             complement(6308..>6484)
                     /locus_tag="PLJ64_09360"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HNC93730.1"
                     /translation="PRLGPSSKDELMAGEIVTVEPGIYISGATGVRIEDLVVVGENGV
                     DRNLSTVTKALTFV"
BASE COUNT         1284 a         2157 c         1937 g         1106 t
ORIGIN      
        1 ggctgaccgg ttccgcgggt ggggcattcc ggatcccctc atcaacgact tccgcagctc
       61 aagccccgac cctcaccagc cgccgcccgc gcacgcaagc cgggcaatcc accccggtct
      121 ggtcatccac cggacccggt ccctcaagcg cggcgaagtg aggaatcgcc acggcattcc
      181 ggtcacatca gcggttcgaa ccgtgatcaa tctggctgaa agggaaccgc ccaggtcgct
      241 cgacaccgcc atcaggagag gcattgccag aaacctcttt gaccttgacg aattgaaccg
      301 ggccattgac cgggcccggg ggcggaaagg catcgcgaaa ctaaggaagg tgctcgcgaa
      361 ctggaacccg ggcaagctca ggaccaaatc gggtctggag aacaagacgg ctgaccggtt
      421 ccgcgggtgg ggcattccgg atcccctcat caacgacttc cgctgcggtc acgaagtcga
      481 ctttcagtgg cccggatcga acatcctggt cgaatccgac ggtggcgcct atcacgcgag
      541 tcgggcggac cggcatcggg actatcagaa gatgctcgat ctccaggcgg caggaaacgt
      601 cgtcgtgcgg atcgatgagg acatggtcga aaaggaggcc tacgaagtgg ggttgaaact
      661 gcgaaagctc ttcgcggaag aaggcgtggt cgagcttcag ccgatttcac cccagccggt
      721 cgcggtcagc gaccgggcgc caccgtgatc ccggtgttcg cagaggtaga tcccctgcca
      781 ggctccaaga tgaaagcacc cgtcccgcac cggaatcgtc aagtcgaatc cggtcactga
      841 acccttgatg tgagccggca tgtcgtcggc gccctcaagg gtgtgcgtcc agcgataggc
      901 ggtcggccgt tccggcacga ccgagtcgat ccatgaactg aagtcccgcc tcacatcggg
      961 cgaggcgttt tcgttcaggg tcaatgaagc cgacgtatgc cggatcagca ggtggatcag
     1021 gccgatccgc agtgttccga gctccgggag gccccgttcg atctcgtccg tgaccaggtg
     1081 aaagcccctg gatcgagcct cgagagtcag atctcgctga aacatctcca tcgcagaacc
     1141 gtacccgccc ggaggtgggt cggataacca gctgccagga cggaaatccg acccacccct
     1201 gcgggaggtt caagcggcgg tgcctggtgg gtcgggattc aggtcctgat gacggaaatc
     1261 cgacccaccc ggtgggcagg ctttgggctg gggcccgggt tgggggcctg ggccgggttc
     1321 gggggtgtcc gggtccgggg gtggccgggg ttcaggcgcg ggacatgtat tcggcagtct
     1381 cggtgttgac cttgatcact tcgccctcgt tgatgaaaag cggcacctgg acctcgactc
     1441 ccgactcaag ggtcgccggc ttcgtgccgc cgcccgaggc ggtgtcgccc ttcaggccgg
     1501 gttcggtctt caccactttc gcttcgatcg agctgccgac cgagacatcg gaggggttct
     1561 cgtccacgaa taggacttcg acttcatcgt tgggctggat ccagcgcatc gcgtccgcag
     1621 cgatatcggc cggaatttcg atctggtcgt agctctggtt gtccatgaag acgaccgcgt
     1681 caccagactc gtagaggtac tgcatccgct ttgactcggt gcgaaccggc cggaacttct
     1741 ccccggcgcg gaaagtcttg tcgatgactc cgccgtcttc gatgcggcga agcttggtgc
     1801 gcacgaacgc accgcccttg ccgggcttca catgctggaa ctcgatgatt cgcatgatct
     1861 tgccttcgac ctcgatgtgg gacccgttgc ggaaatcgct ggttgaaatc acggcagaca
     1921 ctttagccac agggcgcccg ggcgagtcgc gccgaacggc ataatcaatc catgactacg
     1981 actcccgcgc ttctcgacga attgctccgc acccccggcc cttccggcta cgaagctccc
     2041 gccgccgacg tctggcgcaa ggcggccggt ttcgccgaga tcaccaccga cggtctcggt
     2101 tccagcgcgg cgacaatccc gagttcggac gaaaatgacg aagcgcccct cgtcgcggtg
     2161 gtcggccaca tcgacgagat cggcctggtc atcacccaca tcgacgaaaa gggcttcctt
     2221 tacttcgcgc cgatcggcgg ctgggatccc cagatcctgg tcgggcagcg cgtcacgatc
     2281 accggcaacg aaggtcccgt gcccggcgtc gccgggcgca agccgatcca cctgctcgag
     2341 ccggatcagc gcaagaaggt cgtccagctg aaggagatgc acatcgacat cggcgccgat
     2401 gaccgcgagg acgcgcagaa gatggtcagc gtcggagacc cggtcgtgat cgactgcgaa
     2461 ccgcaggcga tcgccggagg acgcctcgtt tcccgctcga tggacaaccg gctggggtcc
     2521 tatgtggcgc tcgaggcggc ccggcggatc cacgagaacg ccagggcaaa ggttcgattc
     2581 gccggcgtcg ccgcggttca ggaggaaatc ggcctgttcg ggtcccgcac cgccgccttc
     2641 acctttcgac ccgatctcgc cgtagcgatc gacgtcaccc acgcgaccga cgctccaggc
     2701 atcgacgaaa aggaagtcgg cagtcacccg ttcgggtcgg gaccggtgat cggacggggt
     2761 tcgaccctct cccccaaggt ctacgaactg ctccgcgacg ccgcggaaaa ggccggtatc
     2821 gattactcgg taggcgccag tggaaactcg acccataccg atgccgactc tcttcagatt
     2881 tcccggaccg gcatcccgac gggtctcgtc tcgatcccgc tgcgctacat gcattcgccg
     2941 gtcgagatgg tcgacctggc cgacgtcgag gcaacggtgg aactcatggt cgcgttcgtc
     3001 gaaggcctcg cggtggatgt ggacctctct cgctgaaccc ttctttccgt acggaatcgt
     3061 tcagaaacat cctgtccgat cggcgcaact tccgccatga atccggtcta gtatctgtga
     3121 gtcaagtcac acgaccggtg tttccggtcg gccacaaatc gcggccggtg ttacagccgg
     3181 tcaaagctat gagagggagt tacccggaga gatgaatcac caattgggaa taacgaatgc
     3241 acgccgtttc gtggcgctcg cgttcgccct ggtcatggcc ttcgcgctga cggcagcatt
     3301 tgccaacagc caggccgagg caggaaagaa gaagaaaaag gccaagccgg ccaccgtcaa
     3361 ggtcgtcaac atcacgaccg cgaaccagca ggccctgctc aagcagaaga agctgacggt
     3421 gaaggtcaag tcgaccggca aggcttcacc gaaggtttcc gctgtgagct ccggaaagag
     3481 caactacttc aagtcggtca ccgtcaagtt caagaagaag ggcaccaaga ccgtgagtct
     3541 cgccctgact tctgccggca agaccgccct cggcacctgt ggcgccaaga ctgtccaggt
     3601 caacgcctca tacaagaagg gcaagaaaaa ggccaaggct tcgaagaaga agaccctcgg
     3661 caagtgggct gacaacagca actgcttcgt gacggttgac gttggcgacg atcccgcgca
     3721 ctgtgacttc ctcgacgcga cccagtgcct ccagccctgg ccgaacgatt actacaccaa
     3781 ggaagattcg tcgaccccga ccggcaagcg cctgaacctc ggcgccgagt caacgccgat
     3841 gaataccggc aacgccaccc cggtccatct cgatgtcacc gacatcaacc gcggtgacgg
     3901 cttcagcccc ggcaacgaga tcctgatcaa gattccgggc ctcgacactc cggctgcttt
     3961 caccaacagc aacctggttc cgcttgatga catcagcgcc tacaaggatg cggcccaggc
     4021 cgtgatcgtg atcgacgccg ccaccggcga gcgtcagccg atctgggccg agctcgactc
     4081 gaacccgacc tcggtcaacc cgaccgaaga ggatccaggc ggaatcaaca tcaacccgac
     4141 caacacccag ccggtcaatc tgatcgtcca tccggccaag aacttcgagt tcggccaccg
     4201 gtacatcgtc gccttccgaa acctgaagga cgccgacaac aacgcgatcc agtcgccgct
     4261 cggattccgg gtataccggg acaacctgaa gaccaagcag agcgtcgtcg agaaccgccg
     4321 ttcccacatg aactcggtca tcaacgacct ggtcaacaag gccggcgttg atcgttccag
     4381 cctctacatg gcatgggact tcaccgttgc ctccgaggac agcgtgaccg gccgcgccac
     4441 gacgatccgt gacgacgcct tcgcgcgtct cggcgacaac aatctcgccg atcgccagat
     4501 cgccggcaac agcccggaca tcgacgtcct cgcttactgc gacaagagca acgtcgcggc
     4561 gccgagctgt ggcaacaact accccggcat ccccgagttc aacgggtcga atccggttac
     4621 ctcgccggta ccgggcaccg acatccagcg cacggtcgtc gggtacatcc gcgacgtgcc
     4681 ctgctacctg gatcaggatg gctgccccgc gagcgggtcg cagttcgcct tcgactcgaa
     4741 tggcaacctg acctggaaca acaccttcaa gatggacgtt cccttccggt gcatgatccc
     4801 gtcgtcgatc gtgaacacgg gcaccgtggt tccgggtcac accggcacct acggtcacgg
     4861 tctgctcgga tcgctgaacc agatcaacgc gacagttgaa gccggaaacc ggaccaacac
     4921 ggttttctgc gctgccaact gggatggctt ctcgacgctg gacgccggcg tggtcattca
     4981 gtcgctcatg aacatgagca acttcaacaa gctggtcgac cgcatgcagc agggcttcgt
     5041 gaatttcctg tacctgcagc gggcgctggt ccacccggac ggtttcgccg accaggccgc
     5101 cttccagatg gatcacaacg gagccacccc gatcaccccc ggctccgcga tcgacacgag
     5161 cgccggtgct gccacccgtg gctactacta cgggatcagc cagggcggca tcatgggtgg
     5221 tgcgctgacc gcgctctcgc ccgatgtcga caagggcgtg ctcggagtcc cgggcatcaa
     5281 ctactcgacc ctgctcagcc gctcggttga ctcggacgag tacttccagg ccccgggtta
     5341 cgggctctac ccgaactacc ccgacatgtc gtcccgccag ctgctgctct cgctgatcca
     5401 gctcctctgg gatcgcggtg aaggcaacgg ctacgcgaac aacatgaccg acaacccgct
     5461 gccgaacacc aactcgcacg aggttctgct ccagctggcg cttggcgacc accaggtcac
     5521 caactacgcg gctgaagtcg aggcccggac gatcggtgcg gtgcgctacg cgccgaccct
     5581 gaggccggaa cggacctggg atctcgatta cgaggccctg ccggggaaca ccagtttccc
     5641 gatcggcccg aacgagagct tcatggtcta ctacgacggc ggaccgccct cattcaacgg
     5701 cacccaggac ccgggcacgg cgaagccgcc gctcgagaac gtcccgccgc ggaccgagtg
     5761 gggcttcggc gaggacccgc acgggttccc ccgccgggcg ctggacgcca acctccacat
     5821 caagtcgttc tacgagaacg gaacgatcga cagttgtgag gcgacttcac cgtcgccggg
     5881 cgacgcctac tgctacgcaa acggctggga cggcgtcacc ggtctctagc cagcagcaga
     5941 agcagcattc gagaacgggg ccccgcgggg ccccgttttc gttgcacgag ccggggtggg
     6001 tcggatttcc cgtcccggga ctggaaatcc gacccacccc ggtggaggcg acccggcccg
     6061 gtagaggcga cccaccccgg tggaggcgag ctggctctgt gccggcatcc gggctccgcc
     6121 tcggctgcgg ccgttgatgc aatgaaaaga ccggccccct caggccggtc ttttcgtctg
     6181 cacctctctc ctcaaaaact ccctcgccat attgctctgg agatccctcc ccactcgtga
     6241 cagaagcaat gtcgtctgtg tgacgggtat cacactatag aggatcgtta caaaagatgc
     6301 attcccccta cacgaaggtc agggccttgg tgaccgtgct caggttccgg tccactccgt
     6361 tctccccaac cacgaccaga tcctcgatcc tcacaccggt cgcgccagag atatatatcc
     6421 ccggttcgac cgtgacgatc tcgcccgcca tcagctcgtc cttgctgctc ggaccgagcc
     6481 gggg
//
LOCUS       DAOQKN010000143         6456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_6082_length_6456_cov_5.045149, whole genome shotgun sequence.
ACCESSION   DAOQKN010000143 DAOQKN010000000
VERSION     DAOQKN010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 6456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6456
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6082_length_6456_cov_5.045149"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /locus_tag="PLJ64_09365"
     CDS             complement(<1..907)
                     /locus_tag="PLJ64_09365"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936882.1"
                     /note="catalyzes the reduction of ribonucleotides to
                     deoxyribonucleotides; the rate-limiting step in dNTP
                     synthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HNC93731.1"
                     /translation="MSQSVSESTRVNPDGVVVERRLTREGVHPFDEVEWEIRDAIIGD
                     PAKPAFEQRGVEFPRTWSQNATNIVSQKYFRGQLGSPERENSVKQMIGRVAGTIANWG
                     REGGYFASDADADIFEAELTATLLHQEVAFNSPVWFNVGFEETPQCSACFILSVEDTM
                     DSILDWNTKEGNIFRGGSGSGINLSNIRGSMEPLKKGGTASGPVSFMRGADSWAGTIK
                     SGGKTRRAAKMVVLDVDHPDVEHFINCKADEEKKAGALRDAGFDMSIDGDGFTSIQYQ
                     NANNSVRVSDEFMQAVADDAEWNLLA"
     gene            complement(1054..1512)
                     /gene="nrdR"
                     /locus_tag="PLJ64_09370"
     CDS             complement(1054..1512)
                     /gene="nrdR"
                     /locus_tag="PLJ64_09370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HNC93732.1"
                     /translation="MRCPHCGHQGSRVLESRSSEEGGAVRRRRECSSCKHRFTTYERY
                     ETQSLFVIKRDGGRQPFDRAKLRGGLERASHKRPVRPEAIDDLVNRIEKKAVRDGGQI
                     EAARIGDMCLAGLRRIDQVAYLQFAAVYRQLGVEDVQAELDRLSPQLSKN"
     gene            1676..2542
                     /locus_tag="PLJ64_09375"
     CDS             1676..2542
                     /locus_tag="PLJ64_09375"
                     /inference="COORDINATES: protein motif:HMM:NF014997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HNC93733.1"
                     /translation="MSQEPRNARERATQARESAVTRATAARDGAVERAGNARDAAVER
                     AGVVRDATVEMAGNARVAAVEKAAAVKDSATESIKAIKPKFRGVSHEWAFFLSLGLGI
                     SLLMLADTPKKSLAVGIYAVSLCALFGTSALYHRINWKTVKSRMLMRRLDHSMIFLLI
                     AGTVTPFALLNMTGTWSTAILVAVWSAAIVGIIVELLWIHSPKWVSAAMYVAVGWIGA
                     VAFPQIVGTAGVWAGLLIATGGILYTVGAVVYATGRPNPSPTYFGYHEVFHLFVIGAA
                     ALHFAAVAMFAL"
     gene            2562..3407
                     /locus_tag="PLJ64_09380"
     CDS             2562..3407
                     /locus_tag="PLJ64_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNC93734.1"
                     /translation="MTLEGAPEWSSIAVGDHWIRTYQAGPHQASEAVVFIHGNPGSAA
                     DFTGLIDAVAPPRRAIAFDLPDFGQSIAAPGFGHTKDEYSHFVGDAFKALGIRRAHLV
                     LHDLGGPIGLDWASTHTGQIASVTLINTGVLAGYKWHRTARVWQTPVAGELLQAAAFR
                     SMFRRIITRAEPHGLPRDFVDGMYDNYDRRTRRAVLDIYRDLKKVGKTSKQLIPLLAA
                     ADLPCLVVWGAGDPYLPASFADRQRSAFPRAQVHILPASGHWPFIDDPPAVTELLTAF
                     LATVE"
     gene            3556..5106
                     /locus_tag="PLJ64_09385"
     CDS             3556..5106
                     /locus_tag="PLJ64_09385"
                     /inference="COORDINATES: protein motif:HMM:NF018561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HNC93735.1"
                     /translation="MNHRRITATVCCLMVALAGAFALAGPASAAYDPNDPAQKAEYDA
                     AFDLGLQAYEFGLPLLDMKRTYLSSTSVNWPSGRGGGPVNMFSHFRKLADAKDRTVVL
                     PNSDTLYSMAWLDLSRGPVVIHSRKNTRRFHVFELLDPWTENFANIGSPEGAKPDGDY
                     MVASKWWRGKVPKGLKLIRSPYDRVWVIGRTLVKSKADLKNVWKIQNTYRIVPLKKWN
                     PKRPEAYHPKKPKKLDLVKNEAHIPGTGEGEDPADFFDALGIALARFKAPARDETILA
                     RMLVGLGIGPGHFPVRNDELSDPQLAALRDVVTGAPSRMQSGLLSTYIQDFDIHNGWL
                     VARTGRYGTDYHRRAQISRFGLGAPTPNVAMYPVALMDRNKAPLTGAKRYVVHFDPDQ
                     AKPPVKFFWSLTLYDNDSFFVDNPIDRYLVNDRSNLKYNDDGSLDIYIQSEAPADPGQ
                     MGNWLPSPAPDAAQPAFRLTVRLYGLSKQGIRNLYSGEGWQLPTILPCGVGNQTSTGV
                     ACATTTPG"
     gene            complement(5109..6062)
                     /gene="rsgA"
                     /locus_tag="PLJ64_09390"
     CDS             complement(5109..6062)
                     /gene="rsgA"
                     /locus_tag="PLJ64_09390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNC93736.1"
                     /translation="MIDPRLAEIGWHKDDQIAWGEVPREDQIPARVVGLHRNHIVDLL
                     TAEGELAGRPAGRMLQDRSSATAMPAVGDWVAALPDGTIQEILPRRSALARRSAADRD
                     RIQILATNIDKAIVISSLNREHDPVRLERMVRLAESSGAEPVIGLSKSDLTDEARELK
                     ERVSQAFPEVKVFVFSSRNGEGVDAVREFLEPGETVVMLGRSGVGKSTLANTLLGWER
                     QKTAEIRGTDDRGRHATTSRELFPLPGGALLIDTPGLRAPGSIEADDLIPGSAESDLT
                     DERAREIERLAAYCRFRDCAHETEPDCAVTAAIEAGELPAR"
     gene            complement(6093..>6456)
                     /locus_tag="PLJ64_09395"
     CDS             complement(6093..>6456)
                     /locus_tag="PLJ64_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HNC93737.1"
                     /translation="LVNVVDVPPLCNFILPAIVRTGPLAIAISTAGASPALAKRMKNE
                     IAAEYGEDHARLAVILNEARGWAKSTLPTYQDRKVFFEGIVNGEPDPIALIREDRESE
                     LLELIENAKREAEASLAA"
BASE COUNT         1020 a         2184 c         2078 g         1174 t
ORIGIN      
        1 gggcgagcag gttccactcg gcgtcgtcgg cgaccgcctg catgaactcg tcggagaccc
       61 ggaccgagtt gttggcgttc tggtactgga tcgaggtgaa accgtcaccg tcgatcgaca
      121 tgtcgaagcc ggcgtcacgc agcgcgcccg ccttcttttc ctcgtccgcc ttgcagttga
      181 tgaagtgctc gacgtccgga tgatcgacgt cgaggacgac catcttcgcg gcccgccggg
      241 tcttgccgcc ggacttgatc gtgcccgccc aggagtcggc gccgcgcatg aaggagaccg
      301 ggccggaagc ggtgccgccc ttcttcagcg gctccatcga accgcggatg ttggagaggt
      361 tgatgccgga gcctgaaccg ccgcggaaga tgttgccttc cttggtgttc cagtcgagga
      421 tcgaatccat cgtgtcctcg accgagagga tgaagcaggc cgagcactgc ggggtctcct
      481 cgaaaccgac gttgaaccag accggcgagt tgaaagcgac ttcctgatgg agcagggtcg
      541 cggtcagttc cgcctcgaag atgtcggcgt cggcgtcgga ggcgaagtag ccgccctcgc
      601 gaccccagtt agcgatcgtg ccggccacgc ggccgatcat ctgcttgacc gagttctccc
      661 tttccggcga gccgagctgg ccccggaagt acttctggga gacgatgttg gtcgcgttct
      721 gcgaccaggt cctggggaac tcgaccccgc gctgctcgaa tgcgggcttg gcgggatcac
      781 cgatgatcgc gtcgcggatt tcccattcga cttcatcgaa ggggtggacg ccctcgcggg
      841 tgaggcgcct ttcaacgaca actccgtcgg ggttgacgcg ggtcgactcg gatactgact
      901 gggacatact tatgctctct cctcgctgct tggaacgact ggtctgcttg cttctcttgc
      961 tggtactgcc cgaatctctt gtgactggat gacgcggtga ctcgtggtgc tgtggtgttg
     1021 ctttcctgca ggtactgctg tggctcttga agttcagttc ttgctgagct gcgggctcag
     1081 ccggtcgagt tccgcctgga cgtcctcgac cccgagctgg cggtagaccg cggcgaactg
     1141 caggtatgcg acctggtcga ttcgccggag ccccgccagg cacatgtcgc cgatgcgggc
     1201 tgcttcaatc tgaccaccgt cccggacggc ttttttctcg atccggttga ccaggtcatc
     1261 gatcgcctct ggccggaccg gacgtttgtg cgaggcgcgc tccagccccc cgcgcaactt
     1321 ggcgcggtca aagggctggc gcccgccatc ccgcttgatg acgaacaggg actgtgtctc
     1381 gtaccgctcg tatgtagtga agcggtgctt gcagctggag cactcgcgcc gacggcgcac
     1441 agcgcccccc tcctcggagc ttcggctttc gaggacacga cttccctgat gtccgcaatg
     1501 cgggcaacgc acatggcaaa ttatactagg actagggtcg gatacctttt tcgcacgcta
     1561 tatgtggtgt ttcgcaggaa aactgtttcc gggttttgcg ggaacggcac accacgcttt
     1621 gcccatgccg gctcggtttc cgggtcactt gccccgggcc ggtacgattc atccagtgag
     1681 tcaggaaccc cgaaatgcac gcgaaagggc gactcaggcc agggagtccg cggtgacccg
     1741 tgcgaccgcc gcccgggatg gtgccgtcga gcgggccggg aatgcccgcg acgccgccgt
     1801 cgagcgggcc ggcgtggtcc gtgacgccac ggtcgagatg gccggaaacg cgcgcgtcgc
     1861 ggcggtcgaa aaggcggccg cggtcaagga ttcggcgacc gaatcgatca aggcgatcaa
     1921 gcccaagttc cgcggcgtct cccacgaatg ggcttttttc ctctcgctcg ggctcgggat
     1981 ctcgctgttg atgctggccg acaccccgaa gaagtccctg gcggtcggga tctacgcggt
     2041 ttcgctctgt gcgctcttcg ggaccagcgc cctctatcac cggatcaact ggaagacggt
     2101 caaatcgagg atgctgatgc gccggctcga ccactcgatg atcttcctgc tgatcgccgg
     2161 caccgtgacc cccttcgcgc tgctcaacat gaccggcacc tggtcgaccg cgatcctggt
     2221 cgcggtctgg tcggcggcga tcgtcggcat catcgtcgag ctgctctgga tccactcgcc
     2281 gaagtgggtc agcgccgcga tgtacgtcgc ggtgggctgg atcggtgcgg ttgcctttcc
     2341 ccagatcgtc ggcaccgccg gggtctgggc gggcctgctg atcgccaccg gcggcatcct
     2401 ctacacggtc ggagcggtgg tctatgcgac cggccggccg aatcccagcc cgacctactt
     2461 cggctaccac gaggtctttc acctcttcgt gatcggcgcc gccgccctgc atttcgcggc
     2521 cgtcgcgatg ttcgccctct gatgaaccga cgggacgcga cttgactctc gaaggcgctc
     2581 ccgaatggtc cagcatcgcg gtcggcgatc actggatccg cacctaccag gccgggcctc
     2641 accaggcctc cgaggcggtc gtcttcatcc acggcaatcc gggttcggcc gcggacttca
     2701 ccggactgat cgacgccgtc gcgccgcccc gccgggcgat tgccttcgac ctgccggact
     2761 tcggccaatc gatcgcggcg ccgggattcg gtcacaccaa ggatgagtat tcgcacttcg
     2821 tcggcgacgc gttcaaggcg ctcgggatcc ggcgggctca tctcgtgctc cacgatctcg
     2881 gcggaccgat cgggctcgac tgggcgtcga cccacaccgg gcagatcgcc agcgtcaccc
     2941 tgatcaacac cggggtcctg gccgggtaca agtggcatcg aaccgcccgg gtctggcaga
     3001 ccccggtcgc cggcgagctg cttcaggccg ccgccttccg ctcgatgttt cggcggatca
     3061 tcacgcgggc cgagccgcat ggcctgccgc gcgatttcgt cgacgggatg tacgacaact
     3121 acgaccgtcg cacccgccgg gccgtgctcg acatctaccg tgacctgaag aaggtcggca
     3181 agacctcgaa gcagctgatt cccctcctgg ccgccgccga cctgccctgc ctggtcgtct
     3241 ggggcgcggg ggatccctac ctgccggcct ccttcgccga tcgccagcgg agcgccttcc
     3301 cccgggccca ggtccacatc ctgccggcct ccggccactg gcccttcatc gacgaccctc
     3361 cggccgtcac cgagctcctg accgcctttc tggccaccgt cgaatagccc cgccaggcgt
     3421 cggggtcttc ggccggcgaa cccgggtaac ggtctcaacc gggtccgcag cttttcgctc
     3481 ccggacgggt tagttgacgt acatcacttt tcgggcaaga atgttccgag gaggaatcgc
     3541 tttgaggaga gtgtcttgaa ccaccgccgc atcaccgcaa ccgtctgttg tctcatggtc
     3601 gccctggctg gtgccttcgc cctggccggt ccggcaagcg ccgcctacga cccgaacgac
     3661 cccgcacaga aggccgagta cgacgctgcc ttcgatctcg ggctccaggc ctacgaattc
     3721 ggcctgcccc tgctcgacat gaagcggacc tacctttcca gcacctcggt caactggccg
     3781 agcggtcgcg gcggcggccc ggtcaacatg ttcagccact tccgcaagct ggccgacgcc
     3841 aaggaccgca cggtggtcct gcccaattcg gacactctct actcgatggc ctggctcgac
     3901 ctcagccgcg ggccggtcgt gatccactcc cgcaagaaca ccaggcggtt ccacgtcttc
     3961 gagctgcttg atccctggac cgagaacttc gccaacatcg gctcgcccga gggcgccaaa
     4021 ccggacggcg attacatggt ggccagcaag tggtggcgag gcaaggtgcc gaagggtctg
     4081 aagctgatcc gctcgcccta tgaccgcgtc tgggtcatcg gccgcaccct ggtcaagagc
     4141 aaggccgacc tcaaaaacgt ctggaagatc cagaacacct accggatcgt gccgctgaag
     4201 aagtggaatc cgaaacggcc cgaggcctac cacccgaaga agccgaagaa gctcgacctg
     4261 gtcaagaacg aagcccacat tccgggaacc ggtgagggtg aggatccggc cgacttcttc
     4321 gatgcgctcg gcatcgcgct ggcccgcttc aaggcgccgg cgcgggacga gacgattctg
     4381 gcccggatgc tggtcggcct gggcatcggc cccggtcact tcccggttcg aaacgacgag
     4441 ctctcggatc cgcaactcgc ggccctgcgt gatgtcgtga ccggcgcgcc gtcccgcatg
     4501 cagtcgggcc tgctctccac ctacattcag gatttcgata tccacaacgg ctggctcgtc
     4561 gcccggaccg ggcgctacgg aaccgactat caccgccgcg cccagatctc ccggttcggc
     4621 ctcggcgccc cgaccccgaa cgtggcgatg tacccggtcg cgctgatgga ccgcaacaag
     4681 gccccgctga ccggggccaa gcgctacgtg gtccacttcg acccggacca ggccaagccg
     4741 ccggtcaaat tcttctggtc gctgaccctc tatgacaacg acagcttctt cgtcgacaac
     4801 ccgatcgacc gttacctggt caacgaccgt tcgaacctca agtacaacga cgacggttcg
     4861 ctggacatct acatccagtc ggaagcgccc gccgatcccg gtcagatggg caactggctg
     4921 ccgtctccgg cgcccgacgc cgcccagccc gccttccgcc tgacggtccg cctctacggc
     4981 ctttccaagc aggggatccg gaacctctac agcggagagg gctggcagct cccgaccatc
     5041 ctcccctgcg gggtcggcaa ccagacatcg accggcgtcg cctgcgctac caccaccccg
     5101 ggctgaggtc agcgggcggg gagctcgccc gcttcgatcg cggcggtgac cgcgcagtcg
     5161 ggctcggttt cgtgggcgca gtcgcggaag cggcagtagg cggcgaggcg ttcgatctcg
     5221 cgggcccttt cgtccgtgag gtccgattcg gccgaaccgg ggatcaggtc gtcggcttcg
     5281 atcgagccgg gggcacggag gccgggcgtg tcgatcagga gcgccccgcc gggtagcgga
     5341 aacagttcgc ggctggtcgt ggcgtggcgg ccgcggtcat cggtgccgcg gatctcggcg
     5401 gtcttctggc gctcccagcc gagcagggtg ttcgcgaggg ttgacttgcc aaccccggaa
     5461 cggccgagca tcaccaccgt ttcgcccggc tcgaggaact cgcgaaccgc gtccacgcct
     5521 tccccgttgc gggaactgaa gacgaagacc ttgacctccg ggaacgcctg gctgacccgt
     5581 tccttgagtt cgcgggcttc gtcggtcaga tccgatttgg aaagcccgat caccggctcg
     5641 gcccctgagg attcggccag ccggaccatg cgctccagcc ggaccgggtc atgctccctg
     5701 ttaagtgagc tgatcacgat cgccttgtcg atgttggtgg cgaggatctg gatccggtcg
     5761 cgatcggcgg cgctgcgacg ggccagcgcg ctgcggcggg gcaggatctc ctggatcgtc
     5821 ccgtccggca gggcggcgac ccagtcgcca acggcaggca tcgcggtggc cgaagagcgg
     5881 tcctggagca ttcggcccgc cggcctgccg gccagttcgc cttcggctgt gagcaggtcg
     5941 acgatgtggt tgcgatgaag gccgacgacc cgggccggaa tctggtcttc ccggggaacc
     6001 tcgccccagg cgatctgatc atccttgtgc cagccgatct cggccaggcg ggggtcgatc
     6061 atgcagggaa acccggggtc atgcgaagaa ggctacgccg caagtgacgc ctccgcctcc
     6121 cgcttggcgt tttcgatcag ctcgagaagt tcgctctcgc ggtcctcgcg gatcagggca
     6181 atcgggtccg gctcaccgtt gacgatgcct tcgaagaaga ccttgcggtc ctggtaggtc
     6241 ggcagggtcg acttggccca gccacgggcc tcgttgagga tcaccgccag ccgggcgtgg
     6301 tcctcgccgt attcggcggc gatctcgttc ttcatccgct tggcgagtgc gggggaggca
     6361 ccggcggtcg agattgcgat cgccaggggg ccggtccgga cgatcgccgg caggatgaag
     6421 ttgcagagcg gcggcacgtc aaccacgttg accagc
//
LOCUS       DAOQKN010000144         6412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_6132_length_6412_cov_5.424729, whole genome shotgun sequence.
ACCESSION   DAOQKN010000144 DAOQKN010000000
VERSION     DAOQKN010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 6412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6412
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6132_length_6412_cov_5.424729"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="PLJ64_09400"
     CDS             <1..344
                     /locus_tag="PLJ64_09400"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNC93738.1"
                     /translation="VGVDLLFQAVDFPELVIVGGHIGYPWTEEMVAVCRKHENVYIDT
                     SAYTPKRYPRELIDYMKTKSGRRKVLWGSNFPMIGPAAALEGIGELGLDEETTGLFLG
                     GNARRVFGLPG"
     gene            complement(369..1172)
                     /locus_tag="PLJ64_09405"
     CDS             complement(369..1172)
                     /locus_tag="PLJ64_09405"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNC93739.1"
                     /translation="MAGSKGKQFSRFVAIGDSQTEGVGDPTGPGGLERGWADRFAERL
                     DLTRRATGEAGLLYANLAVRGKLLAQIDEDQIEPALAMEPDLVSLIGGLNDVIRPGCD
                     IDLVLARMDTMQGRLAASGATVLTITYPDPALMMPLGRLLSDTMAEFNRGLRRIAGRH
                     GALVLDVAESAEVTDPDHWCDDRLHLNSTGHQVMADGMFSLIEPLPEGQSWLGEITPR
                     QLHGLHERVMAEARWAGAFLAPWIYRRMVGKSSGDGRLAKRPELTPVEV"
     gene            complement(1213..2829)
                     /locus_tag="PLJ64_09410"
     CDS             complement(1213..2829)
                     /locus_tag="PLJ64_09410"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNC93740.1"
                     /translation="MAKPPNLLLIITDQQRYPMHWPEDQEWLDELMPADAELARTGVT
                     FEETCIASCMCSPSRASLFTGRWPAEHGVDLTLTRGGAEIDRSHSKHALRKGIEAVRH
                     GEMSALEMVTNTVKGAARRANGGTDERELDPNMENLPSVLKRAGYKTVLKGKWHLTQP
                     VGSDWSQADAERLADEYGFEGWVPPDAGENIEPSHFGGGNKAGKSKEGYDEDFTRQAE
                     EFLADPPDEPWALIFSLVNPHDVLGYPSSYEEGGYSDREWADLDAVQLPPSVDETMKN
                     KPRVHSLLKIGQLSFLGPIEGEQRLEYCRFYAHLHRLADAKIGRVLEALGDPEDPDSL
                     RSKTVIFRTSDHGDLGMSHGGLRQKMFNAYEETVNVPLIVSNPQMFPEARRTNAPASL
                     CDVLPTMAELAGADTEGLELRGRSLVPVLKEEAESVQEGTLFTYDDHQSGTAYRDVTP
                     GANRIRAWRTPEATYAIYLNPHGGPPEFELYDRTRDPDQVDNLVDRDSGRAINPADRE
                     LLEKMRAGLKAEMRRCRVPALPATIDRLPA"
     gene            complement(2859..4214)
                     /locus_tag="PLJ64_09415"
     CDS             complement(2859..4214)
                     /locus_tag="PLJ64_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93741.1"
                     /translation="MTAVKKVFLAAIALLLAGTAIAIPARAALVENGYNPLDLPTAGC
                     FWTGPFTAANEKTNTAYPGTEITYWGAKFRTPAGAVLRLRGQYPHARYSSLNAYESSG
                     GVPTGSISDRQIKPDRGSVNTSLPGKDRKAKKRAWTVRVLGSAAPEKPVANTLYAQPS
                     SEDYQDIIYRVYVADKGRSKSGGVPIPTPELTLEDGTVLTGKALCAALNSNHDYKSQS
                     VPTVLYDALVNWPGKDPLTNPAEADFSFIKFFNLQLSLARYKTQAEFDAAWEANPNEV
                     GTLYGNNDARYMTGAFSTRFGKVLVIKGSMPTTPQTYRGQRRAKAGQLVEWDMCTLES
                     VATTKTNKCLFDEQVPLRGKQRRYTIVVAKAGDRPKNATRECGVAFLPADPDGDGVGR
                     KDAGLLLTRNVIPASNFKHSIQNVTTPYNAARVMGRYYPRGTYTSKAAFQKLGCPKAG
                     N"
     gene            complement(4256..5449)
                     /locus_tag="PLJ64_09420"
     CDS             complement(4256..5449)
                     /locus_tag="PLJ64_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93742.1"
                     /translation="MSSPGRFGCVLATVLAGLMVWAAPSGAIPWDLGGAVPPEETTAP
                     VLPDPLCQQSYADDEPVPGPRIHFGVGPRLAGEIGSGQTTPLVPENWQERDRALRRLA
                     GDRDFTVRLNRLFLSDGWRGIRQFQKMARHYGRLGFGVELQVRYHPRPQQNGDIGAWL
                     DYVRDVVRAFGPIKAVRALQITNEVNLTVSPNTSDGAWLNSTRALVAGVKTAQRYSNR
                     LGFGHLKIGFNFAWRFGAEADADFWDRLAAVGGKELRQATDWVGIDLYPGTYLPPRVS
                     ISNYGDAFLEAIAQMRECYMPKAGFGPRFPLKVEETGWPTGPGRSEEEQAQVLRQFVD
                     TAHRYRGTYKVTDFRWFGLRDNNSDGPDYQSYFGLLRSNYSKKPAFYEYLTRVSKLGA
                     GRSSR"
     gene            complement(5446..>6412)
                     /locus_tag="PLJ64_09425"
     CDS             complement(5446..>6412)
                     /locus_tag="PLJ64_09425"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HNC93743.1"
                     /translation="YLPATGPEPVESHPVEVEALAELAAEILAERSGIRESFASEDRE
                     RALGEILRVGTSAGGARAKALLAFNPETMEVRSGQLEVPAGFEHWILKFDGVQDQSRE
                     IGEPRGYGVIEFAYAQMARAAGIEMTDCHLLEDAGRRHFMTRRFDRTESGRRLHMQSL
                     AALGHLDFNLPGANSYEQAFRMIDRLGLGRAAVEQQFLRMVFNVVARNQDDHVKNIAF
                     LMDRGGEWALSPAFDLTWAYNPTGRWTSAHQMSVNGKRDGFEPGDFFEVGKLVSMKRG
                     RVEAMLDKVTESVSAWPELAADAGVEESVIKRIADTHRLDLGQSK"
BASE COUNT          977 a         2122 c         2170 g         1143 t
ORIGIN      
        1 cggtcggcgt cgacctgctc ttccaggctg tcgacttccc cgaactggtc atcgtcggtg
       61 gccacatcgg ctacccgtgg accgaggaga tggtcgccgt ctgccgcaag cacgaaaacg
      121 tctacatcga cacctccgcc tacacgccga agcgctaccc ccgggagctg atcgactaca
      181 tgaagacgaa gtccggccgc cggaaggtcc tctggggatc gaacttcccg atgatcggac
      241 cggccgctgc cctggaagga atcggggaac tcggcctcga cgaagagacc accgggctct
      301 tcctcggcgg caacgcccgc cgtgtcttcg gcctgccggg ctgacctggc gccgggccgc
      361 gagaccgctc agacttcgac cggcgtcagt tcgggccgct tggccaggcg gccatcgccg
      421 gaggacttgc cgaccatgcg gcggtagatc cagggcgcga ggaaggcgcc ggcccagcgc
      481 gcctcggcca tcacgcgctc atgcagcccg tgcagctggc gcggggttat ctcgcccagc
      541 caggactgac cctccggcag cggctcgatc aggctgaaca ttccgtcggc catcacctga
      601 tggcccgtcg agttgagatg cagccggtcg tcacaccagt ggtcggggtc ggtgacctcg
      661 gccgattcgg cgacgtcgag gacgagggcg ccgtggcgcc cggcgatccg ccgcaggccc
      721 cggttgaact cggccatggt gtccgagagc aggcggccga ggggcatcat cagggccggg
      781 tccgggtagg tgatcgtgag aacggtcgct ccgctggcgg caagtcgacc ctgcatcgtg
      841 tccatccggg cgaggacgag gtcgatgtcg caaccgggcc ggatcacgtc attgagaccg
      901 ccgatcaggc tgaccaggtc cggttccatc gccagggccg gctcgatctg gtcttcgtcg
      961 atctgggcga gcagctttcc ccgcaccgcg agattcgcgt agagcagccc cgcttcgccg
     1021 gtggcccgcc gggtcaggtc gagacgctcg gcaaagcggt ccgcccagcc ccgttcgaga
     1081 cctcccggcc cggtcgggtc accgaccccc tcggtctggc tgtccccgat cgccacaaaa
     1141 cgactgaact gcttcccttt gctgcccgcc atgccgaaac cctatccccc aagcggagga
     1201 attcaagggc ggtcaggccg ggaggcggtc gatcgttgcc ggcagggccg ggacgcggca
     1261 gcggcgcatt tcggccttga gaccggcacg catcttctcc agcagttcgc gatcggccgg
     1321 attgatcgcc cggccgctgt cgcggtcgac caggttgtcg acctgatccg ggtcccgggt
     1381 gcggtcatag agctcgaatt ccggggggcc gccatgcgga ttgaggtaga tcgcataggt
     1441 cgcctccggg gtacgccagg cgcggatccg gttggcgccc ggggtgacgt cgcggtaggc
     1501 ggtgccggac tgatggtcgt cgtaggtgaa gagggtccct tcctggaccg actcggcctc
     1561 ctctttcagc accgggacca ggctgcggcc gcggagctcg agcccttcgg tgtcggcgcc
     1621 ggccagttcc gccatcgtcg gcagcacgtc gcagagcgac gcgggggcgt tggtccgccg
     1681 ggcctcgggg aacatctgcg ggttggacac gatcagcggc acgttgacgg tctcctcgta
     1741 ggcgttgaac atcttctgac ggaggccgcc gtgcgacatg ccgaggtcgc cgtggtcaga
     1801 ggtgcggaag atcaccgtct tcgaacggag tgaatccggg tcctcgggat cacccagcgc
     1861 ctccaggacg cggccgatct ttgcgtcggc gaggcgatgc aggtgggcgt agaaacggca
     1921 gtactcgaga cgctgctcgc cttcgatcgg gccgaggaag ctgagctggc cgatcttgag
     1981 cagggaatgc acccggggct tgttcttcat ggtttcgtcg acgctcggcg gcagctggac
     2041 ggcgtcgagg tcggcccatt cgcggtcgct gtagccgccc tcctcgtacg aggaggggta
     2101 accgagcacg tcgtgaggat tgacgagcga gaagatcagc gcccagggct catcgggcgg
     2161 gtcggcgagg aactcctcag cctggcgggt gaagtcctcg tcgtagcctt ccttgctctt
     2221 gccggccttg ttgccgccgc cgaagtggct cggctcgatg ttctcgccgg catccggcgg
     2281 cacccagccc tcgaagccgt attcgtcggc gaggcgctcg gcgtcagcct gggaccagtc
     2341 gctgccgacc ggctgggtca gatgccactt gcccttgagc acggtcttgt agccggcccg
     2401 cttgagcacg ctgggcaggt tttccatgtt cgggtcgagc tcgcgctcgt cggtgccgcc
     2461 gttggcccgg cgggctgccc ccttgacggt gttggtcacc atctccaggg ccgacatctc
     2521 gccatgcctg accgcttcga tgcccttgcg cagggcgtgc ttgctgtgcg agcggtcgat
     2581 ctccgccccg ccccgggtca gggtcaggtc caccccgtgc tcggccggcc agcgccccgt
     2641 gaagagcgag gcacggctcg gggagcacat gcaggaggcg atacaggtct cctcaaaggt
     2701 gactccggtg cgagccagtt cggcgtcggc cggcatcagc tcgtcgagcc actcctgatc
     2761 ctccggccaa tgcatcggat agcgctgctg gtcggtgatg atcagcagca ggttgggcgg
     2821 tttcgccacg ctcttcccct tctttttctt tgccacgctc agttacccgc tttcggacag
     2881 cccagcttct ggaaagcggc cttgctggtg taggtgcccc gcgggtagta acggcccatg
     2941 accctggccg cgttgtaggg cgtggtcacg ttctggatcg aatgcttgaa gttcgaggcc
     3001 gggatcacgt ttctggtcag gagcagcccg gcgtccttgc ggccgacgcc gtccccgtca
     3061 gggtcggccg gcaggaaggc cacgccgcac tcgcgggttg cgttcttcgg ccggtcgccc
     3121 gccttggcga cgacgatcgt gtagcggcgc tgtttgcccc ggagcggcac ctgctcatcg
     3181 aagaggcatt tgtttgtctt cgtggtcgcc accgactcga gcgtgcacat gtcccattca
     3241 accagttgcc cggccttcgc ccggcgctgc ccccggtagg tctgcggtgt cgtcggcata
     3301 ctgcccttga tcaccagcac cttgccgaag cgggtgctga aggcaccggt catgtagcgg
     3361 gcgtcattgt tgccgtaaag cgtgccgacc tcgttcgggt tggcctccca tgcggcgtcg
     3421 aattcagcct gggtcttgta gcgggccagc gagagctgga ggttgaagaa cttgatgaag
     3481 ctgaagtccg cctcggccgg gttggtcagc ggatccttgc cgggccagtt gaccagggcg
     3541 tcatagagaa cggtcggaac gctctgcgac ttgtaatcgt gattcgagtt gagcgccgcg
     3601 cagagcgcct tgccggtcag cacggttccg tcctcaagcg tcagttccgg ggtcgggatc
     3661 ggaacgccgc cggacttgct ccggcccttg tccgcgacgt agacccggta gatgatgtcc
     3721 tgatagtcct cggacgaggg ctgggcgtag agggtgttgg cgaccggctt ttcgggagcg
     3781 gccgagccga gcacgcggac cgtccaggcc cgctttttgg ccttgcggtc cttgccggga
     3841 aggctggtgt tgaccgagcc ccggtccggt ttgatctgac gatcggaaat cgaaccggtc
     3901 ggcacgccac cgctcgattc gtaggcgttg agcgacgagt atcgggcatg cgggtactgg
     3961 ccccgcagcc tcagaaccgc gcccgccggg gtgcggaatt tcgcacccca gtaggtgatc
     4021 tcggttccgg gataggcggt gttggtcttc tcgttcgcgg cggtgaaggg accggtccag
     4081 aagcagccgg cggtcggcag gtcgagcggg ttgtagccat tctcgaccag ggcggcccgg
     4141 gccgggatcg caatcgcggt tccggcaaga agcaaagcga tcgctgccag aaaaaccttc
     4201 ttcactgccg tcatgcctct gctcctgctg tcgaattggt cgccggtccc cgccctcagc
     4261 gggaagacct gcccgcgccc agtttactga cgcgcgtcaa gtattcatag aacgcgggct
     4321 ttttcgaata gttgctgcga agcaggccga aataggactg atagtcggga ccgtcgctgt
     4381 tgttgtcccg gagcccgaac cagcggaaat cggtgacctt gtaggtgccg cggtaacggt
     4441 gcgccgtgtc cacgaactgg cgaaggacct gcgcctgctc ttcctcgctt cgccccggcc
     4501 cggtcggcca tccggtttcc tcgaccttca gggggaagcg gggcccgaag ccggccttgg
     4561 gcatgtagca ctcgcgcatc tgggcgattg cctcgagaaa ggcgtctccg taattgctga
     4621 tcgagacccg gggtggcaga taggtgcccg ggtagaggtc gattccgacc cagtcggtcg
     4681 cctggcgcaa ctcctttccg ccgaccgcgg ccaaccggtc ccagaagtcg gcgtcggctt
     4741 cagccccgaa acgccaggcg aagttgaagc cgatcttgag atggccgaag ccgagccggt
     4801 tcgagtaacg ctgcgcggtc ttcaccccgg cgaccagcgc ccgggtcgag ttcagccagg
     4861 cgccgtccga tgtgttcggg gagacggtca gattgacctc gttggtgatc tgcagggccc
     4921 ggaccgcctt gatcggaccg aaggcgcgga ccacgtccct cacgtagtcg agccaggctc
     4981 cgatgtcacc gttctgctgc ggccgggggt ggtacctgac ctggagctcg accccgaatc
     5041 ccagccggcc gtaatgccgg gccatcttct ggaactgcct tatgccacgc cagccgtcgg
     5101 agaggaagag ccggttgagc cggaccgtga aatcgcgatc gccggccaga cgcctcaggg
     5161 cacggtcgcg ctcctgccag ttctcgggca ccagcggcgt ggtctggccg ctgccgatct
     5221 ctccggccag ccgggggccg accccgaaat ggatccgggg accgggaacc ggctcgtcgt
     5281 cggcgtagct ctgctggcaa agcgggtcgg gcagaaccgg ggccgtcgtc tcctccgggg
     5341 gaacggcccc gcccaggtcc cagggaatcg cgcccgaagg agcggcccag accatcaagc
     5401 cggccagcac cgtcgccagc acgcatccga agcgccccgg cgagctcact tgctctgccc
     5461 cagatcgagg cgatgggtgt cagcgatccg cttgatcacg ctttcttcaa caccggcatc
     5521 ggccgcaagc tccggccatg ccgaaaccga ttcggtcacc ttatccagca tcgcctccac
     5581 ccgaccccgc ttcatcgaga ccagtttccc cacctcgaag aagtctccgg gctcgaaacc
     5641 gtcgcgcttg ccgttgaccg acatctggtg ggcggaggtc cagcggccgg tcgggttgta
     5701 ggcccaggtc aggtcgaagg ccggggaaag cgcccactcg ccaccgcgat ccatcaggaa
     5761 ggcgatgttc ttcacgtggt cgtcctgatt tcgagccacg acgttgaaga ccatgcgcag
     5821 gaactgctgc tcgaccgccg cccggcccag gccgaggcgg tcgatcatgc ggaaggcctg
     5881 ctcgtaggaa tttgcccccg gcagattgaa gtcgaggtgc ccgagggcgg ccagcgactg
     5941 catgtgcagg cggcggccgg actcggtgcg gtcgaagcgg cgggtcatga agtggcgccg
     6001 gccagcatct tcgagcaggt ggcagtcggt catctcgatc cctgccgccc tcgccatctg
     6061 tgcgtaggcg aactcgatca ctccgtaacc gcgcggctcg ccgatctcgc ggctttggtc
     6121 ctggacaccg tcgaacttga ggatccagtg ctcgaacccg gccggcacct cgagctgacc
     6181 ggagcggacc tccatcgtct ccgggttgaa ggcgagcagg gccttggccc gggccccgcc
     6241 ggccgaggtg ccgacccgca ggatctcgcc gagcgcccgt tcccggtcct cgcttgcgaa
     6301 gctttccctg atcccggacc gctcggcaag gatctccgcc gccagctcgg ccagggcctc
     6361 gacctcgacc ggatggctct cgaccggctc tggaccggtc gccggcaggt ac
//
LOCUS       DAOQKN010000145         6384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_6174_length_6384_cov_5.764418, whole genome shotgun sequence.
ACCESSION   DAOQKN010000145 DAOQKN010000000
VERSION     DAOQKN010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 6384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6384
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6174_length_6384_cov_5.764418"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..641
                     /locus_tag="PLJ64_09430"
     CDS             90..641
                     /locus_tag="PLJ64_09430"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNC93744.1"
                     /translation="MPRIPYPTEDELEPEARERMANLPPLNLIRMFANAPTALGPLTD
                     LGQAILLQSELDPRLREVAILAVARACDSEYERLQHENICRAIGMNEEEIRAAADQNP
                     EALDEEARLIWTFADQIATSVRADEELTARVLEKLGRRQTTELVIACAFYSAMGRIIE
                     TTGVELEETLPTENIDPDEYTGD"
     gene            complement(667..1260)
                     /gene="metW"
                     /locus_tag="PLJ64_09435"
     CDS             complement(667..1260)
                     /gene="metW"
                     /locus_tag="PLJ64_09435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HNC93745.1"
                     /translation="MRPDLDIVAGLVGSGQRVLDLGCGDGALLENLISEWDCRGLGVE
                     RELDDFHACIGRGVPVTQGDLEEELPRFGAGDFDVAVLSLTLQAVRRPDRVLTEMKRV
                     APRLVVSLPNFAHWKMRATLALRGRMPVTDALPYQWYDTPNIHLCTIRDFEQLTGELD
                     LKIDRRILIDATGRRTGGLARRAPNLLAERVVYSLTA"
     gene            complement(1257..2450)
                     /locus_tag="PLJ64_09440"
     CDS             complement(1257..2450)
                     /locus_tag="PLJ64_09440"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HNC93746.1"
                     /translation="MPDASSGIGIVETQRAVVFSGDRPLTLASGAILPEVEVAYETYG
                     ELNPDRSNAVFICHALSGDAHAAGLHEGDDPSRPGWWDNIVGPGRPLDTDRFFVISAN
                     VLGGCKGTTGPSSIDPATGKPYGLRFPLVQVRDLVEVHRALLRELGIDHLLAAIGGSL
                     GGMQALQWAIDHPDEVDGAAIIAASARLSAQNIAFSAVAREAIMRDPDFAGGDYYERE
                     RRPDPGLALARMIGHITYLSEESMREKFGRRIQDAEVPRYGFDVDFQVESYLHYQGQS
                     FVERFDANTYLYMGRTMDYFDPFEDPVLVQNQISQGETSFLVISFDSDWRFSTEHARV
                     LASGLRGAGAPVTFQEISSPHGHDSFLFADPPEYHRTVAGYLDRLASQRGIPVPGATS
                     GSREG"
     gene            complement(2451..3746)
                     /locus_tag="PLJ64_09445"
     CDS             complement(2451..3746)
                     /locus_tag="PLJ64_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HNC93747.1"
                     /translation="MSDEEKQYGPETNALHAGQVADPTTNARAVPIYQTVSYEFNDAQ
                     HAQDLFALAEPGNIYTRIMNPTWNVLEERMAALEGGVAALALASGQAAVTYSVLNVCR
                     TGDNIVALSTLYGGTYNLFAHTLPQYGIEVRFVDPDKPEELEKHVDDKTRLVFGETIG
                     NPRLNVMDVEAWANAAHAQGLPLVVDNTVATPVLTRVFELGADIAVHSATKFIGGHGT
                     TIGGVIVDSGNFDWVANADRFPGLTQPDPSYHGVVWADALGPAAYIGRVRTVLLRNTG
                     AALSPFNAFLLLQGVETLPLRMERHSANALAVAEHLSDSENVLWVNYPGLPGFSHHET
                     ASKVLTGGYGALVTFGIKGGRDAGRKFIDSLELFSHLANIGDAKSLAIHPATTTHSQL
                     NDEELEDSGVTADTVRLSVGIETIGDILADIDQALAKAS"
     gene            complement(3783..4421)
                     /locus_tag="PLJ64_09450"
     CDS             complement(3783..4421)
                     /locus_tag="PLJ64_09450"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC93748.1"
                     /translation="MSEGFTHVFEPGDPARTLLLLHGTGGDENDLVSLGRQLAPGAGI
                     LSPRGKVLENGMPRFFRRLAVGELDIPDLLVRTDELADWVTTAAGDYGFDPAGLIGLG
                     LSNGANIAASLLFRRPGTLGAAALLRSMLPYPPPEDLDLQGAPVLVASGGQDQFVPAG
                     ESEELARQLEAHGAKVEFIFDPGAGHGLTQEDLKATFRWLEEIKSASVEEPR"
     gene            4519..5376
                     /locus_tag="PLJ64_09455"
     CDS             4519..5376
                     /locus_tag="PLJ64_09455"
                     /inference="COORDINATES: protein motif:HMM:NF039218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH hydrolase domain-containing protein"
                     /protein_id="HNC93749.1"
                     /translation="MIKGVLAVSLASLLVLAGVARADQGTEWIPPLDAVARDKGEMAF
                     DGCRPKPERIDPVMCVYGDPDSDKTIALFGDSHAMEWGPPLIRLAQRRGWRLVTFLRA
                     GCVIADVKFQNNCDEWRELAFQEIAEQRPNRVIVSTSIGRRYTLSVRGETLSRTESEK
                     ILRRGMARTLKRLASIRSLTPHGTKVKLIRDQITAPFLPPRCLADHPDHPRLCRFPRK
                     RKFGPGFDVVGASKAGQLPAIDPLEALCGPRWCYSSRGNIVIYRDSDHLTATFTRTLT
                     DWLGERIGF"
     gene            5392..6279
                     /locus_tag="PLJ64_09460"
     CDS             5392..6279
                     /locus_tag="PLJ64_09460"
                     /inference="COORDINATES: protein motif:HMM:NF039218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH hydrolase domain-containing protein"
                     /protein_id="HNC93750.1"
                     /translation="MIRSSKGRLAAALAGTAVTVCLLAGCGSDPGPATISGLVPAPEV
                     AKEDKGPAILDGCRSGTFHTEAWSCVYGDPASDRTVVLWGDSHAMQFTSPLVKLAEER
                     GWRLVTMFRGNCLTADVEYRPACDAWRANALERIASERPETVITSTDTGNGYALWRDG
                     NRLTRKASEPMLRRAYARTLRRLRKMTGDRRGAVIVIRDLLRSDFRPPDCLLEHPEDL
                     PACDFDGYRKNPPGFDLAAARQVKGVKLVDPSEIVCPGGICNAARDGMVVYRDTTHIS
                     STYAETLADWLGEQVDSLP"
BASE COUNT         1075 a         2081 c         2215 g         1013 t
ORIGIN      
        1 tccgaggccg agaactggga tggctcggac ccgatccgcg agctgatccc cgacccctga
       61 aaccccgaac accccgagaa aggccgacca tgccccgcat tccctacccg accgaggacg
      121 agctcgaacc cgaagcgcgt gaacggatgg cgaacctgcc gccgctcaac ctgatccgca
      181 tgttcgccaa cgccccgacc gcactcgggc cgctgaccga tctcggtcag gcgatcctgc
      241 tccagtccga actcgacccg cggctccggg aagtggcgat tctggcggtg gcccgcgcct
      301 gcgactccga gtacgagcgc cttcagcacg agaacatctg ccgcgcgatc ggcatgaacg
      361 aggaggagat ccgcgcggcc gccgaccaga acccggaggc actggacgag gaagcgcggt
      421 tgatctggac cttcgccgac cagatcgcca ccagtgtgcg ggccgacgag gaactgaccg
      481 cccgggtgct cgagaagctc ggccggcgcc agaccacaga gctggtgatc gcctgcgcct
      541 tttactcggc gatgggccgg atcatcgaaa ccaccggcgt cgaactcgaa gagaccctcc
      601 ccaccgaaaa catcgacccg gacgaataca ccggggactg agcacgagca aagagaactg
      661 ccgggttcat gcagtcaggg agtagacgac ccgttcggcc agcaggttgg gggcgcgcct
      721 ggcgaggcct ccggtgcggc gaccggtcgc gtcgatcagg atccgccggt cgattttcag
      781 gtcgagctcg ccggtcagct gctcgaagtc gcggatcgtg cagaggtgga tgttcggcgt
      841 gtcgtaccac tggtagggga gggcgtcggt aaccggcatc cgtccccgca gggcgagcgt
      901 ggcgcgcatt ttccagtggg cgaagttcgg cagcgagacg accagccggg gcgcgacccg
      961 tttcatttcg gtcagcaccc ggtcgggccg ccgcaccgcc tgcagggtca gtgaaagcac
     1021 tgcgacatcg aagtcgccgg cgccgaaccg gggcagctct tcctcgaggt caccctgggt
     1081 gaccggcacc ccgcggccga tgcaggcgtg gaagtcatcg agctcccgtt cgacgccgag
     1141 accgcggcag tcccactcgc tgatcaggtt ctcgagcagg gcgccgtcac cgcagccgag
     1201 gtcgagcacc cgctggccgg agccgaccag cccggcgacg atgtcgaggt ccggtctcaa
     1261 ccttcccggc tcccggaagt ggcgccgggg accgggattc cccgctgcga ggcgagccgg
     1321 tccaggtacc cggcaacggt ccggtggtac tcgggcgggt cggcgaagag gaacgagtcg
     1381 tggccgtgcg gggaggagat ctcctgaaag gtgaccggag ctccggcgcc gcgcagcccg
     1441 gaagccagca cccgggcgtg ctcggtcgag aagcgccagt cggaatcgaa ggagatcacg
     1501 aggaaggaag tctcgccctg gctgatctgg ttctggacca ggaccgggtc ttcgaagggg
     1561 tcgaagtagt ccatggtccg gcccatgtac aggtaagtgt tcgcgtcgaa gcgctcgacg
     1621 aaggactggc cctggtagtg aaggtagctc tcgacctgga agtcgacgtc gaagccgtaa
     1681 cgcggcacct cggcatcctg gatccgccgg ccgaacttct cccgcatcga ctcctccgag
     1741 aggtaggtga tgtggccgat catccgggcc agggcaaggc cggggtcggg ccggcgttcg
     1801 cgttcgtagt aatcgccgcc ggcgaagtcc ggatcgcgca tgatcgcctc ccgcgcgacc
     1861 gcggagaagg cgatgttctg ggccgaaagc cgggccgagg cggcgatgat cgcggcgccg
     1921 tccacttcat cgggatggtc gatcgcccac tgcagggcct gcatgccacc gagcgagccg
     1981 ccgatcgccg cgaggagatg atcgatcccg agttccctca gcagggcccg gtggacctcg
     2041 accagatcac gcacctggac cagcgggaag cgcaggccgt acggcttgcc ggtggcaggg
     2101 tcgatggacg aggggccggt cgtgcccttg cagccgccca gcacgttcgc gctgatcacg
     2161 aagaaacggt cagtgtccag cggccggccg ggaccgacga tgttgtccca ccagccgggc
     2221 cgggaaggat cgtcaccctc gtggagcccc gccgcgtggg cgtcgccgga aagtgcgtgg
     2281 cagatgaaga ccgcgttcga gcggtcgggg ttgagctcgc cgtaggtctc gtaagcgacc
     2341 tcgacttccg gcaggatcgc cccggaggcg agggtcagcg ggcggtcgcc ggaaaagacg
     2401 accgcccgct gcgtctcgac aatgccgatg ccgctggaag cgtccggcaa ctagcttgcc
     2461 ttggccaggg cctggtcgat gtcggcgagg atgtccccga tcgtctcgat cccgaccgag
     2521 agccgcaccg tgtccgcggt cacgccggaa tcctcgagct cttcgtcatt cagctgcgag
     2581 tgggtcgtgg tcgccgggtg gatcgccagt gacttggcgt caccgatgtt ggccaggtgc
     2641 gagaacagct ccagcgagtc gatgaacttg cgaccggcat cgcggccgcc cttgatcccg
     2701 aaggtgacca gggcgccgta gccaccggtc agcaccttgc tcgccgtttc gtggtggctg
     2761 aagccgggca ggcccgggta gttcacccag agcacgttct cgctgtcact caggtgctcg
     2821 gccaccgcga gggcgttcgc gctgtggcgc tccatcctca gcggcagggt ctcgacgccc
     2881 tggagcagga ggaaggcgtt gaacggcgaa agggccgcgc cggtgttcct cagcagcacg
     2941 gtccggaccc ggccgatgta ggccgccggg ccgagcgcgt ccgcccagac cacgccgtgg
     3001 taggaggggt cgggctgggt caggccggga aagcgatcgg cgttggcgac ccagtcgaag
     3061 ttgccggagt ccacgatcac gccgccgatc gtggttccgt gaccaccgat gaacttggtc
     3121 gcggagtgga ccgcgatgtc ggcccccagc tcgaagaccc tggtcagaac cggcgtcgcg
     3181 acggtgttgt cgaccaccag cggcaggccc tgggcgtggg cagcgttggc ccaggcctcg
     3241 acgtccatca cgttcagcct gggattgccg atcgtctcgc cgaagaccag ccgggtcttg
     3301 tcgtcaacgt gcttctcaag ctcctcgggc ttgtccggat cgacgaacct gacctcgatc
     3361 ccgtactggg ggagcgtgtg ggcgaagagg ttgtaggtgc cgccgtagag ggtcgaaagg
     3421 gcgacgatgt tgtcgccggt ccggcagacg ttcaggaccg agtaggtgac tgcggcctgg
     3481 ccggaggcca gagcgagggc ggcgacaccg ccttcgagcg cggccatccg ctcctcgagc
     3541 acgttccagg tcggattcat gatccgcgtg tagatgttgc ccggctcggc cagggcgaaa
     3601 aggtcctgcg cgtgctgggc gtcattgaat tcgtaggaga ccgtctggta gatcggaacc
     3661 gcccgcgcgt tggtggtcgg atcggcgacc tgcccggcat ggagggcgtt ggtctcgggt
     3721 ccgtactgct tctcttcgtc gctcattttt ctcctgtggg tgtcatgtga agcggccccg
     3781 aattatcgtg gttcttctac ggaagccgat ttgatttctt ccagccagcg gaaggtggcc
     3841 ttcaggtcct cctgggtcag gccgtgaccg gcgcctggat cgaagatgaa ttcgaccttc
     3901 gcgccgtggg cctcgagctg gcgggcgagc tcctcgcttt ccccggcggg cacgaactgg
     3961 tcctggccgc cggaagcgac cagaacgggc gccccctgca ggtcaaggtc ctccggtggc
     4021 gggtagggga gcatcgatct gaggagagcc gcggccccca gggtgccggg ccgccggaag
     4081 agcaggctgg cggcgatgtt ggcgccgttg gaaagcccga ggccgatcag cccggccgga
     4141 tcgaagccgt aatccccggc ggccgtggtc acccagtcgg ccagctcgtc ggttcgcacc
     4201 agcaggtcgg ggatgtcgag ctccccgact gccagccgcc ggaagaagcg gggcattccg
     4261 ttctcgagaa ccttgccgcg cgggctgagg atgcctgccc ccggggcgag ctggcgacca
     4321 agcgaaacga ggtcgttctc gtcaccgccg gtgccgtgaa gcagcaggag ggtccgggcc
     4381 gggtcgccag gttcgaagac gtgagtgaag ccctcggaca tggatccact ctaaatgagt
     4441 gtctggcccg gccgaaaccc gggccgaaaa cgtgtgtttg atggtcacca gtccattcca
     4501 gtccacaggg agatcaagat gatcaaggga gttctcgcgg tttcactggc ctcgcttctc
     4561 gtcctcgccg gcgttgcccg cgccgatcag ggcaccgaat ggattccgcc gctggacgcg
     4621 gtcgccaggg acaagggcga gatggccttt gacggctgcc ggcccaagcc ggagcggatc
     4681 gatccggtga tgtgcgtgta cggggatccg gattccgaca agacgatcgc cctgttcggt
     4741 gattcacatg cgatggagtg gggtccgccg ctgattcgcc tggcccagcg acgcggctgg
     4801 cggctggtca ccttcctgcg ggctggatgc gttatcgccg acgtcaagtt ccagaacaac
     4861 tgcgacgagt ggcgggaact cgcctttcag gagatcgccg aacagcgacc caaccgggtc
     4921 atcgtctcga cctcgatcgg tcgccgctac acgctctcgg tacgcggcga gaccctctcc
     4981 cggaccgaaa gcgaaaagat cctgcgccgg ggaatggccc gcaccctgaa gcgtctggcg
     5041 agcatccgca gcctgactcc gcacggaacg aaggtgaagc tgatccgcga ccagataacc
     5101 gctcccttcc ttcctccgcg ttgtctggcc gatcatcccg accacccccg tctctgccgg
     5161 ttcccccgca aacgcaagtt cgggcccggg tttgacgtgg tcggcgcgag caaggccggg
     5221 cagctgccgg caatcgatcc gctcgaggcg ctctgcgggc cccgctggtg ctactcgtcg
     5281 cgcggcaaca tcgtgatcta tcgggattcg gaccacctga ccgcaacctt cacccggacc
     5341 ctgaccgact ggctgggaga gcggatcggc ttttagaagt aacttggccg gatgatccgc
     5401 tcctccaaag ggcgtctggc cgccgccctc gccgggacgg ctgtgacggt ctgcctgctg
     5461 gcgggctgcg gcagtgatcc cgggccggcg acgatttccg gcctggtgcc ggcgccggag
     5521 gtggcgaagg aggacaaggg cccggcgatc ctcgacggct gccgctccgg caccttccat
     5581 acggaggcct ggagctgcgt ttacggggac ccggcatccg accggaccgt ggtcctctgg
     5641 ggtgactcgc acgcgatgca gttcacctcg ccgctggtca agctggccga ggagcggggc
     5701 tggcggctgg tcaccatgtt ccgcggcaat tgcctgaccg ccgatgtcga gtaccggccg
     5761 gcctgcgatg cctggcgggc caacgccctg gagcggatcg catccgaacg gccggagacg
     5821 gtcatcacat cgaccgatac gggcaacggc tacgccctgt ggcgggacgg gaaccggttg
     5881 acccggaaag caagcgaacc gatgctgcgg cgcgcctacg cccggaccct gcggcggctt
     5941 cggaagatga cgggggaccg caggggagcg gtgatcgtga tccgcgacct gctgcgttct
     6001 gacttccgcc cgcccgactg cctgctcgag catcccgagg acctcccggc ctgcgacttc
     6061 gacggctacc gcaagaaccc gccgggcttc gacctcgcgg ccgcccgtca ggtcaaggga
     6121 gtgaagctgg tcgacccctc ggaaatcgtc tgcccgggcg gcatctgcaa cgcggcccgc
     6181 gacggcatgg tggtctaccg ggacacgacc cacatcagct ccacctacgc cgaaaccctg
     6241 gctgactggc tgggggagca agtagattcc ctgccatgag caagcacaag catggcaagc
     6301 cgaagccgga gggtgagtgg gtggagcccg acgtccccga attcgtcggc ttcaagaagg
     6361 gcgagaagat ctgggtgcac accg
//
LOCUS       DAOQKN010000146         6336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_6233_length_6336_cov_5.506448, whole genome shotgun sequence.
ACCESSION   DAOQKN010000146 DAOQKN010000000
VERSION     DAOQKN010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 6336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6336
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6233_length_6336_cov_5.506448"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="PLJ64_09465"
     CDS             <1..751
                     /locus_tag="PLJ64_09465"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNC93751.1"
                     /translation="LELAAEISTLPQPALRTDLEAALGGFGHPLEEGLKIEARLFNSL
                     LDQPEIRTGASAFVSREHPDRVAGAAPLHLPGAAYSFAERAHRGQPDKDGTGNFIDHP
                     ARVARIVAGQGGGNIAVAAAYLHDTVENTGVTDGEIEAAFGPGMRELVAALGQDPEID
                     DRDERRRDHRNRVAVAGPTARLVYLADRLDGINAMIGRIEAGEDPEALEVERRISQWR
                     GDVEAMAGLEPPPGLVAEINDRLDLLERLAS"
     gene            875..1954
                     /locus_tag="PLJ64_09470"
     CDS             875..1954
                     /locus_tag="PLJ64_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC93752.1"
                     /translation="MSTSSPSTPADQSEAPDVRLTGLTKRYGNFTAVDAIDLEVRQGE
                     FFTMLGPSGSGKTTTLKMIAGFEQPDAGTVELAGQNVSGLAPYARSVNTVFQDYALFP
                     HMTVAANIEYGLSIAKVPKGERAKRRDEALAMVRLEGHGDRKPAELSGGQRQRVALAR
                     AIVNRPRVLLLDEPLGALDLKLREQMQVELKQIQGEVGITFIYVTHDQDEALTMSDRI
                     AVFNEGRIEQLSSPRDLYEHPGNEFVAGFVGVSNAIERDGRKLTIRPEKIRLIGEGEP
                     ADGLVTERGRIAESAYVGMVTRVKVELEQGGAVQVALQNTESEAEPGGRVGQEVVVGW
                     RPEHAVSIQQGERNQSVQLEEEHVS"
     gene            1951..3147
                     /locus_tag="PLJ64_09475"
     CDS             1951..3147
                     /locus_tag="PLJ64_09475"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNC93753.1"
                     /translation="MSYAHRNPARFAWLLVLAMVAVLAVAGCGSSDDDSSSSSDEVTS
                     IGKTEGALNLIAWAGYAEDGSTDKTVDWVTPFEKQTQCQVNTKTASTSDEMVKLMRTG
                     QYDGVSASGDASVRLIEGGDVSPVDMSILSNYADINPSLKDQDYNSKDGQNYGMPHGW
                     GANVLLYNKDAVSPAPDSWGSVFDPAQASKFSGKITAPDNPIYIADAAVYLEATQPDL
                     GITDPYELDEDQFNAAVDLLKQQNKFVGEYWPDATKNISSYTNGDSVIGSSWQYQTSA
                     LQAAGENVDADLPKEGSTGWSDTWMLSSQAEHPNCMLEWMNWMGSPKVQAQVAEWFGE
                     APANLGACDLTSDPKHCDTYHAKDEKYYDQIAFWKTPISDCGDDRGSECKTYDEWVQA
                     WTEIKG"
     gene            3223..4077
                     /locus_tag="PLJ64_09480"
     CDS             3223..4077
                     /locus_tag="PLJ64_09480"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC93754.1"
                     /translation="MGRRLADFFQGRPKLTVAALLAAPLGWLVIGYLGSLGVMLAASF
                     WHLNELSGEVTQGFSLDNFDTILSDDVYRTITLRTIGVAAAVTVTDAILAFPIAFYMA
                     KLARPKVQSILVVAVLLPLWSSYLVKVGAWRLMLSEDGAINWALEPFGLHGPGYGTVA
                     VWLVMSYIWLPYMIIPIYAGLERIPDSLLSASEDLGAKPGRTFRKVIMPLTFPAIVAG
                     SIFTFSLTLGDYITPANVGDKQFIGNVVYSNFVANLPLAAAITVIPIVVMIIYLLIAR
                     RFGAFEHV"
     gene            4081..4917
                     /locus_tag="PLJ64_09485"
     CDS             4081..4917
                     /locus_tag="PLJ64_09485"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC93755.1"
                     /translation="MTFSPLTKNLLRVAVAITLAFIYIPLLIILIYSTNPNQVLAWPP
                     DGFTLSWFPKAIQDQAARDALWNSVKVGAAATAVALVLGTLASLAVARHKFFGRESIT
                     FLVLIPIALPGIVTGIALNATFTQVFDMKLTLFTVIVAHATFCIVIVFNNAAARLRRM
                     STTLEDASADLGATPWATFRRVTLPNMRSALVAGGLLAFALSFDEIVVTNFTIGAGDQ
                     TLPIYIFNTLFRPNQPGKVYAVAVIAILLSMIPVYIANRLTKEESVTASGAGAGTPIP
                     EP"
     gene            5059..5319
                     /locus_tag="PLJ64_09490"
     CDS             5059..5319
                     /locus_tag="PLJ64_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93756.1"
                     /translation="MERIESRVAKVAKSDKTPKAAAAVVLSGSLLGGADNVQAVTLTA
                     ATLAVAEMARSRFGVKGLRSSGTRSSDKKIGGRIGSVAGRTP"
     gene            5378..5674
                     /locus_tag="PLJ64_09495"
     CDS             5378..5674
                     /locus_tag="PLJ64_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93757.1"
                     /translation="MEVVSLFHSCVYTGLLICAFLLGKPQPVTFILGLSHGLLWIGMS
                     IASILAVHYRVINLRLALAITLLGCIAPFFGTIEFLLQSHRRKLAEADAEAVAS"
     gene            5671..>6336
                     /locus_tag="PLJ64_09500"
     CDS             5671..>6336
                     /locus_tag="PLJ64_09500"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNC93758.1"
                     /translation="MSGHLRLVVTNAMLDGEAVSLTALDGVITAIGPDAGGNSPATAD
                     SSLESLDAGGGILCPPLINGHTHAAMTLFRGHGDDLPLMRWLQEAIWPVEAKLEPEDV
                     YWGTRLACLEMIRSGTIGFWDMYWQPASTARAVEDAGLRAVIGPPLFDAPDKTFEERN
                     RELEGWLDELAGFGDRIAAAVAPHAIYTVGTASLEYAVGLAADRDLPVHIHLSETEGE
                     VNDC"
BASE COUNT         1110 a         2151 c         2021 g         1054 t
ORIGIN      
        1 cctggagctg gccgcggaga tttccaccct cccccagccc gctcttcgca ccgacctcga
       61 ggccgccctg ggcggtttcg gccacccgct cgaggaaggt ctgaagatcg aggccaggct
      121 cttcaacagc ctgctcgacc agccggagat caggaccgga gcgagcgcct tcgtcagtcg
      181 cgagcatccc gaccgggtcg cgggtgcagc cccgcttcac cttccgggcg ccgcctattc
      241 cttcgccgaa cgcgcccatc gcggccagcc cgacaaggac ggaaccggca acttcatcga
      301 ccatccggcc cgggtcgccc ggatcgttgc cgggcagggc ggcggcaaca tcgcggtcgc
      361 ggccgcctac cttcacgaca cggtcgagaa caccggcgtc acggacgggg aaatcgaagc
      421 cgcgttcggt cccgggatgc gggagctcgt ggctgcgctc ggacaggacc cggagatcga
      481 cgaccgggac gaacgccggc gcgaccaccg caaccgggtg gccgtcgcgg gcccgaccgc
      541 gaggctggtc tatctcgctg accggctcga cggcataaac gcgatgatcg gcaggatcga
      601 ggccggagag gacccggagg cgctcgaggt ggagcggcgg atcagtcaat ggcgtggcga
      661 cgtggaagcg atggccgggc tcgagccgcc gcccggcctc gtcgccgaga tcaacgaccg
      721 gctcgacctg ctcgaacgac tcgcctcctg acccgggcgg caggcgaatt tcacagcgac
      781 cgctcccaac ggttagagtc cgattcgacc ggggtagctg gagatctgtc ccgatcagtt
      841 ccgtcaaatc ccgcagtcgc gatcgacgag aggagtgagt acgtccagtc cgtccacacc
      901 cgccgaccag tccgaggcac ccgacgtacg cctgacgggt ctcaccaagc gttacgggaa
      961 tttcactgcg gtcgatgcga tcgatctcga agtccggcaa ggggagttct tcaccatgct
     1021 cgggccgtcc gggtccggca agaccacgac cctgaagatg atcgccggct tcgagcaacc
     1081 cgatgccggc acggttgaac tggccggcca gaacgtcagc ggcctggccc cgtacgcccg
     1141 ctcggtcaac acggtcttcc aggactacgc gctcttcccc cacatgaccg tggcggccaa
     1201 catcgagtac ggcctctcga tcgccaaggt gccgaagggc gagcgggcca aacgccgcga
     1261 cgaggcgctc gcgatggtcc ggctcgaagg ccacggggac cgcaagccgg ccgagctttc
     1321 cggcggtcag cgccagcggg tcgccctcgc ccgggcgatc gtcaaccgcc cccgggtgct
     1381 gctgcttgac gagccgctcg gcgccctcga cctgaagttg cgcgagcaga tgcaggtcga
     1441 gctgaagcag atccagggcg aggtcgggat caccttcatc tacgtgaccc atgaccagga
     1501 cgaggccctg accatgagcg accggatcgc cgtcttcaac gagggccgga tcgagcagct
     1561 gagctcgccc cgcgacctct acgaacaccc gggcaacgag ttcgtggccg gtttcgtcgg
     1621 ggtgtcaaac gcgatcgagc gcgacggaag gaaactgacg atccggccgg agaagatccg
     1681 cctgatcggt gaaggtgaac cggccgacgg cctggtcacc gagcggggcc ggatcgccga
     1741 gagcgcctat gtgggcatgg tgacccgggt caaagtcgaa cttgagcagg gcggtgcggt
     1801 ccaggtcgcc ttgcagaaca cagagtctga agccgagccc ggtgggagag tcggccagga
     1861 ggtggtggtc ggatggcggc cggagcacgc cgtttcgatc cagcaaggag agagaaatca
     1921 gtccgtccaa ttggaggagg aacacgtttc atgagttatg cgcatagaaa cccggcccgg
     1981 tttgcctggc tgctcgtgct ggccatggtg gccgtgctcg cggtcgccgg ctgcggctcg
     2041 agcgacgacg acagcagcag tagcagcgac gaggtgacct cgatcggcaa gaccgaaggg
     2101 gcgctcaacc tgatcgcctg ggccggttac gccgaagacg gctcgaccga caagacggtc
     2161 gactgggtta ccccgttcga gaagcagacc cagtgccagg tcaacaccaa gacggccagc
     2221 acttcggatg agatggtgaa gctgatgcgg accggccagt acgacggtgt ctcggcctcc
     2281 ggcgacgctt cggtgcgcct gatcgagggt ggcgacgtca gcccggttga catgagcatc
     2341 ctctcgaatt acgccgacat caacccgagc ctgaaggacc aggactacaa ctcgaaggac
     2401 ggccagaact acggcatgcc gcatggctgg ggcgccaacg tcctgctcta caacaaggat
     2461 gcggtcagtc cggctccgga cagctggggt tcggtctttg acccggccca ggcgtcgaag
     2521 ttcagcggca agatcaccgc tccggacaac ccgatctaca tcgctgacgc ggccgtctac
     2581 ctcgaggcca cccagccgga tctcgggatc accgacccgt acgagctcga cgaggatcag
     2641 ttcaacgccg ccgtcgatct gctcaagcag cagaacaagt tcgtcggcga gtactggccg
     2701 gatgcgacca agaacatctc gtcctacacc aacggtgact cggtgatcgg ctcgagctgg
     2761 cagtaccaga ccagtgccct gcaggccgcc ggcgagaacg tcgatgcgga cctgccgaag
     2821 gaaggttcga ccggctggtc ggacacctgg atgctgagct cccaggccga acacccgaac
     2881 tgcatgctcg agtggatgaa ctggatgggt tcgccgaagg ttcaggccca ggtcgcggag
     2941 tggttcggcg aggcgccggc caatctcggt gcctgtgatc tgacctcgga tccgaagcac
     3001 tgcgacacct accacgcgaa ggacgagaag tactacgacc agatcgcgtt ctggaagacc
     3061 ccgatcagcg actgcggcga tgaccgtgga agcgagtgca agacgtacga cgagtgggtc
     3121 caggcgtgga ccgaaatcaa ggggtgatga accccttcaa gcacgaagaa gtgcccggga
     3181 ctgttggccg ctgaggccac agtcccggct ccccggaagt cggtgggcag gaggctcgcc
     3241 gacttcttcc aggggcgccc gaagctgacc gtcgcggcgc ttcttgccgc gccgctcggc
     3301 tggctggtca tcggctacct gggctcgctg ggagtgatgc tggctgcctc gttctggcat
     3361 ctgaacgagc tgagcggcga ggtcacccag ggtttcagcc tcgacaactt cgacacgatc
     3421 ctcagcgacg acgtctaccg cacgatcacc ctgcggacga tcggcgtcgc cgcggcggtg
     3481 accgtcaccg atgcgatcct cgccttcccg atcgccttct acatggccaa gctggccagg
     3541 ccgaaggtcc agtcgattct cgtcgtggca gtgctcctgc cgctctggtc cagttacctc
     3601 gtcaaggtcg gcgcctggcg gctgatgctc tccgaggacg gggcgatcaa ctgggcgctt
     3661 gaaccgttcg gactgcatgg ccccggctac ggcacggtcg ccgtctggct ggtaatgagc
     3721 tacatctggc tgccgtacat gatcatcccg atctacgccg gcctcgagcg gatcccggat
     3781 tccctgctca gcgcctccga ggacctcggg gccaaaccgg gccggacctt ccgcaaggtg
     3841 atcatgccgc tcaccttccc ggcgatcgtc gccggctcga tcttcacctt ctcgctaacg
     3901 ctcggggact acatcacccc ggccaacgtg ggtgacaagc agttcatcgg caacgtcgtc
     3961 tacagcaatt tcgtcgccaa tctgccgctg gccgcggcga tcaccgtgat cccgatcgtg
     4021 gtgatgatca tctacctcct gatcgcccgc cggttcggag ccttcgagca tgtctgagcg
     4081 atgaccttca gcccgctgac aaagaacctg ctccgggtcg cggtggcgat caccctggct
     4141 ttcatctaca tcccgctgct gatcatcctg atctactcga ccaacccgaa ccaggttctc
     4201 gcctggccgc ctgatggctt cacgcttagc tggttcccca aggcgatcca ggaccaggcc
     4261 gcccgtgacg cgctctggaa ctcggtcaag gtcggcgccg ctgccacggc cgtcgcattg
     4321 gtcctgggca cgctggcttc gctggcggtc gcccgccaca agttcttcgg ccgggaatcg
     4381 atcaccttcc tggtcctgat cccgatcgcc ctgcccggca tcgtcaccgg tatcgccctg
     4441 aacgcgacct tcacccaggt cttcgacatg aagctgacgc tgttcacggt gatcgtcgcc
     4501 cacgcgacct tctgcatcgt gatcgtcttc aacaacgcgg cggcgcggtt gcggcggatg
     4561 tcgaccacgc tcgaggacgc ttccgccgac ctcggcgcca ccccctgggc caccttccgg
     4621 cgggtgacgc tgccgaacat gcgctcggcc ctggttgccg gcggcctgct cgccttcgcc
     4681 ctctccttcg acgagatcgt ggtcaccaac ttcacgatcg gggccggcga ccagaccttg
     4741 ccgatctaca tcttcaacac cctgttccgg cccaaccagc cgggcaaggt ctacgcggtg
     4801 gcggtgatcg cgatcctgct ctcgatgatc ccggtctaca tcgccaaccg gctgaccaag
     4861 gaggagtcgg tcaccgcctc cggtgcggga gccggaacgc cgattcccga gccgtaacgg
     4921 gctttgcgag gtcgaaccgg agggcgccgg gatgcgtctg gtcggcgata ttcgccgacc
     4981 aagtgcatct cgcggccggc gccggcggat tcacaggatt tcaacttcgc gccctcgttc
     5041 cggtcgtacc ttgatgccat ggaacggatc gaaagcagag tggcgaaggt cgccaaatcg
     5101 gacaagaccc ccaaggcggc agctgcggtg gtgctctccg gatctctcct cggcggagcc
     5161 gacaacgtcc aggcggtcac cctgaccgcg gcaaccctgg ccgttgccga aatggcacga
     5221 agccgcttcg gcgtgaaagg cttgcgctcg agtggcaccc gcagctccga caagaagatc
     5281 ggcggccgaa tcggatcggt cgccggccgc accccctgat cctctctccc cgagggggtt
     5341 cggcacccgt ttcagaaggc tggtcacttt ccatcggatg gaagtggtca gccttttcca
     5401 ttcctgcgtt tacacgggcc ttttgatctg cgcctttttg ctcggcaagc cgcagccggt
     5461 cactttcatc ctcggcctct cccacggcct gctctggatc ggcatgtcga tcgcctcgat
     5521 cctcgcggtc cactaccggg tgatcaacct gcggctcgcc ctcgcgatca ccctgctcgg
     5581 ctgcatcgcc cccttcttcg gcacgatcga gttcctgctt cagagccacc gccggaaact
     5641 ggctgaagcc gatgccgagg cggtggcctc ttgagcgggc atcttcgcct ggtcgtcacc
     5701 aacgcgatgc tggacggcga ggcggtctcg ctgaccgcgc ttgacggagt gatcaccgcg
     5761 atcgggcccg acgccggcgg gaactcaccg gccaccgcgg attcgagcct ggaatcgctg
     5821 gacgccggcg gcggcatcct ctgccctccc ctgatcaatg gccacaccca cgccgcgatg
     5881 accctcttcc gcggtcatgg tgacgacctg ccgctgatgc gctggctgca ggaagcgatc
     5941 tggccggtcg aggcgaagct ggaaccggaa gatgtctact ggggcaccag actggcctgc
     6001 ctggagatga tccgaagcgg cacgatcggc ttctgggaca tgtactggca gcccgcgtcc
     6061 accgcccgcg cggtcgagga tgccggcctc cgcgcggtga tcgggcctcc ccttttcgac
     6121 gctccggaca agacgttcga ggagcggaac cgggagctcg agggctggct ggacgagctg
     6181 gccggattcg gcgaccggat cgccgccgcc gtcgcaccgc acgcgatcta cacggtcggc
     6241 accgccagcc tcgagtacgc ggtcggcctg gccgccgatc gcgacctgcc ggtccacatc
     6301 catctctccg aaaccgaggg cgaggtgaac gactgc
//
LOCUS       DAOQKN010000147         6232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_6389_length_6232_cov_5.259511, whole genome shotgun sequence.
ACCESSION   DAOQKN010000147 DAOQKN010000000
VERSION     DAOQKN010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 6232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6232
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6389_length_6232_cov_5.259511"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="PLJ64_09505"
     CDS             <1..563
                     /locus_tag="PLJ64_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93759.1"
                     /translation="PMKPKPFSEDCVNAAKDAAGFWDPKTKKLMAAFHHFRKRFRKLD
                     RRDDRLTNRIEKLRRNGGPARRIRMLIRVRNRVDNQMFKAGRDYTRRVNPIYMPHVYE
                     TTLILEELLSRRCLDQKKDFIFKNEQKGPVGKVAFRNRILIFTSVLYLSLKVELSGSD
                     SSASASALAVPGPAGLAGLGRSLPLP"
     gene            584..1513
                     /locus_tag="PLJ64_09510"
     CDS             584..1513
                     /locus_tag="PLJ64_09510"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HNC93760.1"
                     /translation="MAGTVEMHPVLRAIAEPAAGDRVVLVIEGGGMRGAVSGGMGLAL
                     HELGLDDRFDAIYGSSAGALNGLWLMSGRAKDGVPTWTDADLGKVVMDKRRIFGSRPI
                     VDMQNVIENHYERISPGLFQAALECGTEFHPLATDIETGQAVDLHGQITDRESLILSL
                     RATSALPLIGGPPVTLNGRRYLDAGLSAAIPFQAAIDDGATHLLVLRSRREGDVAAAP
                     SGIGGSLTARMLGRISPAIRDGYMTRGEREGADEALLDRHHREPGLSPSILQVRPPAD
                     SAVPSRLESDMKVVRTGLEAGRRAVHQLFSTES"
     gene            complement(1510..3627)
                     /locus_tag="PLJ64_09515"
     CDS             complement(1510..3627)
                     /locus_tag="PLJ64_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93761.1"
                     /translation="MLPVFLGVACLPFFASMASAGLVRTDSWGGGLVDPGFVVQSVAY
                     DSQGRAYIGESGKARVIRLAADGSVDLNWSVGQLPGEDEKKANPVDIAIDDEDNVYVL
                     DQIEQMVRKYTADGSLLDQWSAAVPCCLDVEGITVEAGSVFVISGYDVYRYTPSGVLL
                     DSWRGFDSYPEWEQPQIRGDGEGHLLIGSGRFLDEFKANKSIYKFEPDGTMISQWTTP
                     AQGWVYYSQDEWGIYENLADIDGFDAVVPMPDGNPWTAAGGRIARYGSTGEFGDFLDF
                     DRKRFPNALALAPDGGLLYSWAEIPNYPEVSGSGVAKLDPDLDTTRVWTESDFPKYAS
                     NTWDGKFGHALGLTVRTDGVVGGYDTNMDRAQLFDSDGTFIKRIEGPQPNIDSDETPE
                     FLLPEAGGAEFFDPGSGRLSRYDADGDLVDTITLETTGNFEDIALDPEGGYIVLTTVT
                     DRVGQVTFFDQQGEQTSGFKSVRSLASYNGSSLHVEPGAGGIYVQGTGYVEKYSRAGK
                     RLATGVVRGKGCDSAYNYDGLATDLAGNVYLVSEGSQAPMVIKFNRSLKRLWEEKVPK
                     VRWYTGFSGDLDIGPDGSIFMESDKGIMRYDQSKASARPTHEACPKPFHLLRIARTPG
                     RRWAIAAVHVPSSGLLTVSGRKVRTAKRRVGDYGDYGLAIRPKARFTRAARPGFRRFP
                     VTITYKGRHSGKIHTAVKLRVGR"
     gene            3704..5260
                     /locus_tag="PLJ64_09520"
     CDS             3704..5260
                     /locus_tag="PLJ64_09520"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNC93762.1"
                     /translation="MKWIVVAGVALACLLGMADASFGAGLKRITPEEYFGSDPVFLTS
                     PPGDSRLFVVERGDSSTHQGMVRIIENGQPLAEPFLTVDNVGLENERGLMSLAFAPDY
                     ATSGLFYVFYVVNESGDAFGGDGGDIRIVEYQVSAGDPDKADPASARLVFSTPHYTTY
                     HQGSWMAFGPDGHLYFTIGDDGYGPNAQLKDNYFGKIMRIDPADPDGNGPASHTVPAD
                     NPFVAEAGTRPEIYAYGLRNPFRASFAPDGRLVVGDVGNATWEEINAGYLKGANLGWP
                     TCEGFCTSPNPLLTDPIHSYAHDASNCAVIGGYVLQDPGMGSLAWRYLYGDLCNPNLR
                     TLDLDAAGADPKPAGFRIPAGGGTLRSFGEDSAGCVYALTSLAVFRVVSDTATGSGCP
                     GPADAVPAATYNSFIPHRQVIGKRITVAAKCSIACAASATAKVRISRNRFRRNQTVFN
                     LKAGTRNLIAGTRSSLFFRLPSARVATMRKAAGNGSRVTAWVKVSMTGEDGSGGSGSG
                     QIRLTRPVSR"
     gene            5271..>6232
                     /locus_tag="PLJ64_09525"
     CDS             5271..>6232
                     /locus_tag="PLJ64_09525"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNC93763.1"
                     /translation="MKWITAAGVVLVCWLGLSGSAFGASLVRVTPEGYLGSDPSFVTA
                     PPGDSRVFLAERGDAGTHQARIRIIKDGVPVTEPFLTIGNVDLASERGLLSIAFAPDY
                     ASSGLFYAFYVAHGADGFGGNTGDIRIVEYKVSAGDPDQADPASARLVFSIPHSAGNH
                     NGGWMAFGPDDDLYFSIGDNATSSNAQALANYYGKIMRINPADPDGAGPATYSVPTDN
                     PFTSQAGAKKEIYALGLRNPYRASFTPDGRLVIGDVGNGTWEEVDVGDLKGANLGWPT
                     CEGFCTGLTPSFVAPIYAYNHDNGVDGQGGGCAVIGGYIVRDETL"
BASE COUNT         1086 a         2072 c         1997 g         1077 t
ORIGIN      
        1 cgccgatgaa gccgaaaccc ttctcggagg attgtgtcaa tgcggcgaag gacgccgccg
       61 ggttctggga tccgaagacg aagaagctga tggcggcgtt ccaccatttc cggaaacggt
      121 tcaggaagct tgaccgccgc gatgaccggc tcaccaaccg gatcgagaag ctcaggcgaa
      181 acggcggccc ggccaggcgc atccggatgt tgattcgggt tcgaaaccgg gtggacaacc
      241 agatgttcaa ggcgggaagg gactacaccc ggcgcgtgaa tccgatctac atgccgcatg
      301 tgtacgagac gaccctgatc ctcgaggagc tcctttcgcg gcgctgcctc gaccagaaga
      361 aggacttcat cttcaagaac gaacagaagg ggccggtcgg aaaagttgcg ttccgcaacc
      421 ggatcctgat cttcacctcg gtgttgtacc tgtccctgaa agtcgaactc tccggttcag
      481 acagcagcgc ttcggcctcg gctctcgccg tacccggccc ggccggactt gcgggcctgg
      541 gccgcagcct cccgctgccc tgaggaaagc catttcggga tccgtggcag gcaccgtcga
      601 aatgcatccc gtgctgaggg cgatcgcgga acccgcggca ggggaccggg tcgtgctggt
      661 gatcgaaggc ggggggatgc gcggggcggt ttccgggggg atgggcctgg ccctccatga
      721 gcttggcctc gacgaccggt tcgacgcgat ctacggctca tcggctggag cgctgaacgg
      781 cctctggctg atgagcggac gggcgaagga cggggtgccg acctggaccg acgcggacct
      841 cgggaaggtc gtgatggaca agcggcggat cttcgggtcg cgaccgatcg ttgacatgca
      901 gaacgtgatc gagaaccact acgaacggat ctcccccggc ctgttccagg cggcgctcga
      961 atgcgggacc gaattccatc ccctggccac cgacatcgaa accggacagg cggttgacct
     1021 tcacggccag atcaccgacc gggaatcgtt gatcctttcg ctgcgggcga cctcggccct
     1081 gccgctgatc ggcggcccgc ccgtcaccct gaacggccgc cgttacctgg acgccgggct
     1141 cagcgccgca atcccctttc aggcggcgat cgacgacggt gccacccacc tgctggtgct
     1201 gcgctcacgc agggaaggtg atgtcgccgc tgctccctcc gggatcggcg ggagcctcac
     1261 cgcccggatg ctcgggcgga tcagcccggc gatccgcgac ggatacatga cgcggggcga
     1321 gcgggaagga gccgacgagg ccctgcttga ccgccaccac cgtgaacccg gactcagccc
     1381 gagcatcctg caggtccggc caccggcaga ttccgccgtg ccttcacggc ttgaatcgga
     1441 catgaaagtc gtgcggaccg gactcgaagc gggccggcgg gctgttcacc agctcttctc
     1501 gaccgagagc tagcggccga cccgaagctt cactgcggtg tggatcttcc cggagtgccg
     1561 gcccttgtag gtgatcgtga ccggaaagcg ccgaaaaccc ggccgggccg cccgggtgaa
     1621 gcgggccttc gggcggatcg ccaggccgta gtcgccgtaa tccccgaccc ggcgtttcgc
     1681 ggtcctgacc ttgcggccgc tgacggtcag aaggcccgag ctcgggacat gaaccgcggc
     1741 gatcgcccat ctgcgccccg gagtcctggc gatccgcagc agatggaaag gcttcggaca
     1801 ggcctcatgg gtcggccggg cgcttgcctt tgactggtcg tagcgcatga ttcccttgtc
     1861 tgactccatg aagatcgagc catccgggcc gatgtccagg tccccggaaa accccgtgta
     1921 ccacctgacc ttgggcacct tctcctccca aagccgtttc agggagcgat tgaacttgat
     1981 caccatcggc gcctggctgc cctcgctgac gagatagacg ttccccgcca ggtcggtggc
     2041 gaggccgtcg tagttgtagg ccgagtcgca tcccttgccc cgcacgactc cggtcgcgag
     2101 gcgcttgccg gcccgcgagt acttttcgac gtagcccgta ccctggacat agataccgcc
     2161 ggctcccggt tcgacatgca ggctggatcc gttgtagctg gcgagcgaac ggaccgactt
     2221 gaatccggaa gtctgctcac cctgctggtc gaagaaggtg acctggccga cccggtcggt
     2281 gaccgtggtc aaaacgatgt agccgccttc cggatccagc gcgatgtcct cgaagttgcc
     2341 ggtcgtctcc agggtgatgg tgtcaaccag atcaccgtcg gcgtcgtagc ggctcagcct
     2401 gcccgagccc ggatcgaaga actctgcgcc gcccgcctcc ggcaggagga attccggggt
     2461 ctcatccgaa tcgatgttgg gctgcggtcc ttcgatccgc ttgatgaaag tcccgtccga
     2521 gtcgaatagc tgggcccggt ccatgttggt gtcatacccg ccaaccactc cgtcggtccg
     2581 gacagtaagg ccgagggcgt ggccgaactt gccgtcccag gtgttgctcg cgtacttggg
     2641 aaaatcggac tcggtccaga cccgggtcgt gtccagatcc ggatcgagtt tggccacgcc
     2701 ggaacctgac acctcggggt agttcggaat ctccgcccag gaatagagca gcccgccgtc
     2761 cggggccagt gccagtgcgt tcgggaagcg cttgcggtca aagtcaagga agtcgccgaa
     2821 ctcgccggtt gatccgtatc tggcaatccg gcctccggcg gcggtccacg ggtttccgtc
     2881 gggcatcggg accaccgcgt cgaaaccgtc gatgtcagcc aggttctcgt agatgcccca
     2941 ctcgtcctgg gagtaataga cccagccctg ggcgggggtg gtccactgcg agatcatcgt
     3001 gccgtcgggt tcaaacttgt agatcgactt gttcgccttg aattcgtcga gaaacctgcc
     3061 gctgccgatc agcaggtggc cttcaccatc gccccggatc tgcggctgct cccattccgg
     3121 gtacgagtca aaccccctcc agctgtccag caggaccccg gacggcgtgt agcggtaaac
     3181 gtcgtagccg ctgatcacga aaaccgaacc ggcttccacg gtgatgcctt cgacgtcaag
     3241 gcagcaggga acggcggcgc tccactgatc cagcagggag ccgtctgccg tgtacttcct
     3301 gaccatctgt tcgatctggt cgaggacgta gacgttgtct tcatcgtcga tcgcgatgtc
     3361 gaccgggttg gccttcttct cgtcttcgcc aggcagttgc ccgacactcc agttgaggtc
     3421 gaccgaaccg tcggcggcca ggcgaatcac cctcgccttg ccggattccc cgatgtaggc
     3481 gcgaccctgg ctgtcatagg cgaccgactg gaccacgaac ccgggatcga cgagaccccc
     3541 gccccaggag tcggttctga ccagtccggc cgaagccatt gaggcgaaga acggcaggca
     3601 ggcgaccccg agaaacacgg gcagcaacag gcgaagggga cctttcggac gaaaccggtt
     3661 cacacaaatc acaacccgac ggcctcgatg tagtttctgt ggcatgaagt ggatcgttgt
     3721 cgcaggggtg gctttggctt gcctgctcgg aatggccgat gcgtcattcg gggccgggct
     3781 gaagcggatc actccggagg agtatttcgg gtccgacccg gtatttctga cttcaccccc
     3841 cggtgattcg aggcttttcg tggtcgagcg gggtgacagc tcgacccatc aggggatggt
     3901 gcggatcatc gagaacggcc agcccctggc cgagcccttt ctgaccgtgg acaacgtcgg
     3961 cctcgaaaac gagcgcggcc tgatgtcact ggccttcgcc ccggactatg caaccagcgg
     4021 gctcttctac gtcttctacg tggtcaacga gtcgggcgac gccttcggcg gcgatggcgg
     4081 tgacatcaga atcgtcgagt accaggtctc ggcgggtgac ccggacaagg cagacccggc
     4141 ctcggcccgg ctcgtgttca gcacccccca ttacacgacc tatcaccagg gcagctggat
     4201 ggccttcggc ccggacggcc acctctactt cacgatcggc gacgacggat acggcccgaa
     4261 cgcccagcta aaggacaact acttcggcaa gatcatgcgg atcgacccgg ccgaccctga
     4321 cgggaacggg ccggccagtc acacggtgcc cgccgacaac ccgttcgtcg cggaggccgg
     4381 taccaggccg gagatctacg cctacggact tcgcaacccg ttccgcgcct cgtttgctcc
     4441 cgacgggagg ctggtggtcg gtgacgtcgg caacgccacc tgggaggaaa tcaacgccgg
     4501 ctacctgaag ggtgccaacc tcggctggcc gacctgcgaa ggattctgca cttccccgaa
     4561 cccgcttctc accgacccga tccactcata cgcgcatgac gcgagcaact gtgcggtgat
     4621 cggcggctac gtgctccagg accccggtat gggctcgctt gcctggcgat acctctacgg
     4681 cgatctctgc aatccgaacc tccgcacact cgacctcgac gcggccgggg ccgacccgaa
     4741 gccggccggt ttcaggatcc ccgccggtgg cggaacgctg cgttccttcg gtgaggattc
     4801 cgccggttgc gtttacgcgc tgaccagcct cgctgtcttc cgggtggtca gtgacaccgc
     4861 cacagggtcc ggctgcccgg gccccgccga cgcggtgccg gccgcgacct acaactcgtt
     4921 cattccccac cgccaggtga tcggcaaacg catcacggtc gccgcgaagt gctcgattgc
     4981 ctgcgccgcc tcggccaccg cgaaggtccg gatcagccgg aaccggttca ggaggaacca
     5041 gacggtcttc aacctgaagg ccggaacccg gaacctgatt gccggaaccc gcagcagcct
     5101 ctttttccgg ctgccgtcgg ctcgggtcgc cacgatgagg aaggcggccg gcaacgggag
     5161 ccgcgtcacc gcctgggtga aggtctcgat gaccggcgag gacggctccg gaggatcggg
     5221 tagcggacag atccgtttga cccggcccgt ttcgcggtag tttcgtaacc atgaagtgga
     5281 tcactgcggc gggggttgtg ctggtttgct ggctggggct cagcggttcg gccttcgggg
     5341 cgagcctggt ccgggtcaca cccgagggat atctcggctc cgatccgagt ttcgtgaccg
     5401 cgcctcccgg tgactcgcgg gtcttcctgg ccgagcgtgg cgatgcgggc acccaccagg
     5461 cccggatccg gatcatcaag gacggggtgc cggtgaccga gccgttcctc acgatcggca
     5521 acgtcgacct ggccagtgaa cgggggctgc tctcgatcgc cttcgccccc gattacgcat
     5581 ccagcggtct cttctacgcc ttctatgtcg cccacggggc ggatggcttc ggcggcaaca
     5641 cgggcgacat caggatcgtc gagtacaagg tctcggccgg tgatccggac caggccgacc
     5701 ccgcctcggc ccggctcgtg ttcagcatcc cgcactcggc cggcaaccac aacggcggct
     5761 ggatggcctt cggcccggac gacgacctct acttctcgat cggtgacaac gccaccagct
     5821 cgaacgccca ggccctggcc aactactacg gcaagatcat gcggatcaac ccggccgatc
     5881 ccgacggggc cgggccggcc acctactccg taccgaccga caatcccttc acctcacagg
     5941 ccggagcaaa aaaggagatc tacgcgctcg gcctccgcaa cccctaccgg gcctcgttca
     6001 cacccgacgg ccggctggtg atcggtgatg tcggcaacgg aacctgggag gaggtcgacg
     6061 tcggcgacct gaagggcgcc aacctgggct ggccgacctg cgagggcttc tgcaccgggc
     6121 tcaccccctc cttcgtcgcg ccgatctatg cctacaacca cgacaacggc gtcgacggcc
     6181 agggcggtgg ctgcgcggtg atcggcggtt atatcgtccg ggacgagacg ct
//
LOCUS       DAOQKN010000148         6200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_6434_length_6200_cov_5.326282, whole genome shotgun sequence.
ACCESSION   DAOQKN010000148 DAOQKN010000000
VERSION     DAOQKN010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6200
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6434_length_6200_cov_5.326282"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..897)
                     /locus_tag="PLJ64_09530"
     CDS             complement(142..897)
                     /locus_tag="PLJ64_09530"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HNC93764.1"
                     /translation="MLRIGHKGADALVPGNTVASFEKAVEVGVDLIEFDVLWTEDGHP
                     SIPAGKRSQLVVAHDWHAAAAAGERGERLTLEAALEAFARPPLDRVRINLDIKLPGRD
                     YEVFDAVRRHRLIERTSVSTMEISTVRKLGQLKREDEVESGLSLGWTVPKVTKDWLGM
                     PKFIRPALAMGLASVRVRMPGQVRRGLPELEVDSIWAFFGVVTPRLVETIHDAGKKLY
                     VWTVDDPKRIDSLTVLGVDGICSNDPRLLNQAT"
     gene            complement(903..2792)
                     /gene="ilvD"
                     /locus_tag="PLJ64_09535"
     CDS             complement(903..2792)
                     /gene="ilvD"
                     /locus_tag="PLJ64_09535"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNC93765.1"
                     /translation="MPDLRSKTVTHGRNMAGARALLRASGVAREDFGKPIVAVANSYT
                     QFVPGHTHLKPVGEVVSAAIKEAGGVPLEFNSIAVDDGIAMGHGGMLYSLPSRDLIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLNAALRLNIPTVFVSGGPMEGGEATLVDGTV
                     RKRVNLISAIADAVADEVSDEDIAIIEENACPTCGSCSGMFTANSMNCLTEALGLSLP
                     GNGSTLATHTARKALYESAGRTAVEIAGKYYGEDDESVLPRSVATRDAFENAMTLDIA
                     MGGSTNTVLHLLAAAREAGVDFEMSDIDELSKRVPCICKVAPNGHFLMEDVHRAGGIP
                     AILGELRRGGLLNEEVHTVHSPDIDTWLGSWDIRGPKPSEIALDLFHAAPGCKRSSSA
                     FSQSERWESLDTDAAEGCIHDVEHAYSKDGGLAILYGNIAERGCVVKTAGVDESILKF
                     NGPAFVVESQDDAVDAILSGRVKSGDVVVIRYEGPRGGPGMQEMLYPTSYLKGLGLGK
                     ECALLTDGRFSGGTSGLSIGHVSPEAAAGGAIALVETGDEIAIDIPNRTISLEVEEET
                     LAARREAIESSGGYQPVDRDREVSPALRAYAMMATSADTGAVRDVDGVERLVNAARLL
                     DSEQV"
     gene            2810..>3348
                     /locus_tag="PLJ64_09540"
     CDS             2810..>3348
                     /locus_tag="PLJ64_09540"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC93766.1"
                     /translation="MTIPAQEWTELSQQEKRARALDAADRLFAREGLDAPMPALAEAL
                     GIGVGSIYRQVGTKNELIGALVIERSKTLCRRFDATLAEPDPWQAMTAAVHETVDDCL
                     NDALTQSAWDQAAIVSDEVRAARQQATDSLAAMFERARRSGAITAEASPEDLRLLFFA
                     LREFSSVGSAQAHRFTDLVL"
     assembly_gap    3349..3448
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3614..4039
                     /locus_tag="PLJ64_09545"
     CDS             3614..4039
                     /locus_tag="PLJ64_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93767.1"
                     /translation="MKRNYFLKTIGASDHLLSDDWTQTDPHLLSSVRFPKRPSSIKRG
                     DFLVYYATGEQRIFAIGRAVGDGADAAHEIAPGEARWPYLLKIQVFLLIPKLALAPKW
                     DSMGIESTSIMQKSHIEINQRQYEKAWEAIVKRTRLNDS"
     gene            complement(4260..5285)
                     /locus_tag="PLJ64_09550"
     CDS             complement(4260..5285)
                     /locus_tag="PLJ64_09550"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNC93768.1"
                     /translation="MTITESSAQTTIPHDYSKRAMVFGGRSSQDLAAKIAGKLSLAVG
                     DVTLKTFSDGEIYGRYEESIRGADVFLVQSTCANVETDMTPNDALMELMIMADAAQGA
                     SAHRIIAVMPWFGYARQDKKSAPREPITSRVVARSLEAVGIDRVLTMDLHAGQIQGFF
                     TVPVDHMTAQPMLTQWFADQYDPSELVIVSPDAGRAKAARNFARKVGTEWAIMEKERP
                     KQQVAEIGYVVGDVKDKIAIISDDMIDTAGTLCAAARTVLDEGAKSVIACATHGVFSG
                     PAFERLPYEKSGLEKIVVTDTIPLRPGAPDNIHVLSTAGTLADSIRRIFTDDSVSEIF
                     AGENQLF"
     gene            complement(5282..>6200)
                     /gene="glmU"
                     /locus_tag="PLJ64_09555"
     CDS             complement(5282..>6200)
                     /gene="glmU"
                     /locus_tag="PLJ64_09555"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HNC93769.1"
                     /translation="TREELAIREINSGTYVFDGESLASALGELSSDNAQGEYYLTDVI
                     AVMRSRGKLVTAHVSKDPAINLGVNNRADLAVVEAEARQRILRRHMLDGVTIVDPATT
                     WIDAGVTIGQDSRIEPGTSLRGATEIAEEAVIGPHSTIIDSGIGKGCQVIQSYLNLCE
                     LAEGCSVGPFAYLRPGAKLEDGAKAGTFVEIKNSTIGKNSKVPHLSYIGDTEIGENSN
                     LGAGTITANYDGAEKHRTKIGSDVKIGVDTMLVAPVEVGDGAYTGAGAVIRDNIPPGA
                     LSVSENDQRNVDDYAERLADRKAEGESSQ"
BASE COUNT         1071 a         1969 c         1986 g         1074 t
ORIGIN      
        1 ggagtggcag cgcttcatcg agatcaacgc gaactactac aaggacaagt agtccggcga
       61 acctcgcctc gccgccgggc cggaccggcc cggtcacgac ctcggttcaa ttcacttgga
      121 cataacccgg gcgaattgaa cctaggtggc ctgattgagg aggcgggggt cgttcgagca
      181 gatcccgtca acccccagca cggtcaagga gtcgatccgc ttcggatcgt ccacggtcca
      241 gacatacagc ttcttgccgg cgtcgtggat cgtttccacg aggcgcggag tgaccactcc
      301 gaagaaggcc cagatcgaat cgacctccag ctcgggcagg ccgcggcgga cctggccggg
      361 catccgcacc cggaccgaag ccagacccat ggccagggcc ggccggatga acttcggcat
      421 gccgagccag tccttcgtca ccttgggcac cgtccagccg agactgagac cggactcgac
      481 ttcgtcttcg cgcttgagtt gcccgagctt cctcacggtt gagatttcca ttgtggatac
      541 cgaggtgcgc tcgatcagcc ggtgtcggcg caccgcatca aagacctcgt agtcacggcc
      601 cggcagcttg atgtcgaggt tgatccggac ccggtccaga ggcggccggg cgaaggcctc
      661 cagagctgct tccagggtca gcctctcccc cctttccccg gcggccgcgg cggcgtgcca
      721 gtcatgggcg acgaccagct ggcttcgctt gcccgcgggg atcgaagggt ggccgtcctc
      781 ggtccagagc acgtcaaact cgatcaggtc gaccccgacc tcgaccgcct tctcgaagga
      841 agcgaccgtg ttccctggca ccagcgcatc ggcccccttg tgaccgatac gaagcaccgc
      901 agctacacct gctcggagtc gagcaggcgg gcggcgttga ccagtcgctc caccccgtcg
      961 acgtcgcgga ccgcgccggt gtcggccgag gtcgccatca tcgcgtaggc ccgcagggcc
     1021 ggtgaaactt cgcgatcacg gtcgaccggc tggtagccgc cggagctctc gatcgcttcg
     1081 cgtcgggcgg ccagggtttc ctcctcgacc tcgagcgaga tggtccggtt ggggatgtcg
     1141 atcgcgatct cgtcaccggt ctcgaccagg gcgatcgcgc cgccggccgc cgcctcgggg
     1201 gagacgtggc cgatcgaaag gccggaggtg ccaccggaga aacgcccgtc ggtgagcagg
     1261 gcacattcct ttccgagccc cagacccttc aggtaggagg tcgggtagag catctcctgc
     1321 atgccgggac cgccacgcgg gccttcgtaa cggatcacga ccacgtcgcc ggacttgacc
     1381 cggccgctga ggatcgcgtc gaccgcgtcg tcctgggatt cgaccacgaa ggccggcccg
     1441 ttgaacttga ggatcgattc gtcgaccccg gccgtcttga ccacgcagcc gcgctcggcg
     1501 atgttgccgt aaaggatcgc caggccgccg tccttcgagt aggcgtgctc gacgtcatgg
     1561 atgcagccct cggccgcgtc ggtgtcaagg gactcccagc gctccgattg cgagaaggcc
     1621 gacgaagagc gcttgcagcc gggcgccgcg tggaagaggt cgagcgcgat ctcggagggc
     1681 ttcggcccgc ggatgtccca gctgccgagc caggtgtcga tgtccggcga atggacggtg
     1741 tggacttcct cgttcagcag tccgccgcgg cgcagttcgc cgaggatggc gggaatgccg
     1801 ccggcccggt ggacgtcctc catcaggaag tgcccgttcg gcgcgacctt gcagatgcag
     1861 ggcacccgct tcgaaagctc gtcgatgtcg gacatctcga agtccacccc cgcctcgcgg
     1921 gccgccgcca gcaggtgcag gacggtgttg gtcgagccgc ccatggcgat gtccagggtc
     1981 atcgcgttct cgaatgcatc gcgggtcgcg accgatcggg gcagcaccga ctcgtcgtct
     2041 tcgccgtagt acttgccggc gatctcgacc gcggtgcggc cggccgattc gtagagcgcc
     2101 ttgcgggcgg tgtgggtcgc cagggtcgaa ccgttgccgg gcagcgagag accgagcgcc
     2161 tcggtcaggc agttcatcga gttggcggtg aacatgccgg agcaggaacc gcaggtcggg
     2221 caggcgttct cctcgatgat cgcgatgtcc tcgtcggaga cctcgtcggc gaccgcgtcg
     2281 gcgatcgccg agatcaggtt gacccgcttg cggacggtgc cgtcgaccag ggtcgcctcg
     2341 ccgccttcca tcgggccgcc ggagacgaag acggtcggga tgttgagccg caaggccgcg
     2401 ttcagcatgc ccggcgtgat cttgtcgcag ttcgagatgc agaccatcgc gtcggcacag
     2461 tgggcgttga ccatgtactc gacgctgtcg gcgatcaggt cgcgggatgg cagcgagtag
     2521 agcatgccgc cgtggcccat cgcgattccg tcatcgaccg caatcgagtt gaactcgagc
     2581 ggcacaccgc cagcctcctt gatcgccgcg gagacgacct cgccgaccgg cttcaggtgg
     2641 gtgtgaccgg gaacgaactg ggtgtacgag ttggcgaccg ccacgatcgg cttgccgaag
     2701 tcctcacggg ccactccgga agcgcgcaga agcgcccggg ccccggccat gttgcgtcca
     2761 tgagtaacgg tcttcgatct cagatctggc atgtggctaa gatagcggaa tgaccattcc
     2821 ggcacaagag tggacggaac tttcccagca ggagaagcgg gcccgggcgc tggatgcggc
     2881 cgaccgcctc tttgcccggg agggtctcga cgccccgatg cccgcccttg ccgaggcact
     2941 cgggatcggg gtcggcagca tctaccggca ggtcggcacc aaaaacgagc tgatcggcgc
     3001 cctggtcatc gagcggtcca agaccctctg ccggagattc gatgccactc tcgccgaacc
     3061 cgatccctgg caggcgatga cggcagcggt tcacgaaacg gtcgacgact gcctgaacga
     3121 cgcgctcacc cagtcagcct gggaccaggc cgcaatcgtc agcgacgaag tccgcgccgc
     3181 ccgccagcag gcaaccgact ccctggccgc gatgttcgaa agggcccgtc gatccggagc
     3241 gatcaccgcg gaagcctcac ccgaagacct ccgcctcctc ttcttcgccc tccgagaatt
     3301 cagctcggtc ggatcagccc aagcccaccg cttcaccgac ctcgtcctnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnga gaattcagct cggtcggatc agcccaagcc
     3481 caccgcttca ccgacctcgt cctgcgcgga atcaagagct agttccagcc ttctcatgaa
     3541 caacggccga tgacctgtca gaaagggcaa gtgaaaacga ctaaatgagt ccagctacca
     3601 agagagaatc ctcatgaagc gaaactactt cctcaagacc atcggagcga gcgaccactt
     3661 gctcagtgat gactggaccc agaccgaccc tcacctgctc tcctcggtcc gcttcccgaa
     3721 acgcccgtcc agcatcaaga ggggtgattt tctcgtctac tacgcaactg gcgaacaacg
     3781 catctttgca attggaaggg cggtcggcga tggtgcggat gccgctcacg agatcgcacc
     3841 gggcgaagcc agatggccat atctgctgaa gattcaagtc tttctgttga ttccgaagct
     3901 ggcgctcgca cccaaatggg actcaatggg gattgagtcg accagcatca tgcagaagtc
     3961 acacatcgag atcaatcagc gtcaatacga aaaggcttgg gaggcgatcg tcaagcgaac
     4021 tcgcctcaac gattcttagt caagaaggcg gaatccgacc tcggtgcgcc tgcggccgaa
     4081 cttcgttcac ggcggtgcaa gtcaaactca agttccgcgg aaagccttgg ccgcgaagtt
     4141 gggggcgacg aagaattgtc agagcctgca cctgaatcga cgcctagttc gagatggcga
     4201 aagtgagcgc tatatggcca cttttgcctt ctcgattcct gcgcgactgc agttaagccc
     4261 taaaacaact ggttttcgcc ggcgaagatc tcgcttaccg agtcgtcggt gaagatgcgg
     4321 cggatcgagt cggcgagggt tccggcggtg ctgaggacgt ggatgttgtc cggggcgccc
     4381 gggcggagcg ggatcgtgtc ggtgacgacg atcttctcga ggcccgactt ctcgtagggc
     4441 agccgctcga aggcggggcc ggagaagacg ccatgggtgg cgcaggcgat caccgacttg
     4501 gcgccctcgt cgaggacggt gcgggcggcg gcgcagagcg tgccggcggt gtcgatcatg
     4561 tcgtcggaga tgatcgcgat cttgtccttc acgtccccga ccacgtagcc gatctcggcg
     4621 acctgctgct tgggccgctc cttctccatg atcgcccact cggtcccgac cttgcgggcg
     4681 aagttgcggg cggccttggc gcgaccggcg tccggggaga cgatcaccag ctccgaaggg
     4741 tcgtactggt cggcgaacca ctgggtcagc atcggctggg cggtcatgtg gtcgaccggg
     4801 accgtgaaga agccctggat ctggccggcg tgaaggtcca tggtcaggac ccggtcgata
     4861 ccgacggctt cgagtgaacg agcaaccacg cgggaggtaa tcggctcgcg gggggccgac
     4921 ttcttgtcct ggcgggcgta cccgaaccag ggcatgacgg cgatgatccg gtgggcggag
     4981 gcgccctggg cggcgtcggc catgatcatc agttccatca gggcgtcgtt cggcgtcatg
     5041 tccgtctcga cgttggcgca ggtcgactgg acaaggaaga cgtcagcgcc gcgaatcgat
     5101 tcctcgtagc ggccgtagat ctcgccgtcg gaaaaggtct tcagggtgac gtcaccgacc
     5161 gcaagcgaaa gcttgccggc gatcttcgcg gccaggtcct gggagctccg gccaccaaac
     5221 accatcgccc gcttcgaata gtcgtgcggg atggtcgtct gggctgaact ctcggtaatg
     5281 gtcactgact gctttcccct tctgccttgc ggtcggcgag ccgctcggcg taatcatcaa
     5341 cgtttctctg atcgttctcg ctcaccgaga gggccccggg aggaatgttg tcgcggatca
     5401 ccgcgccggc gccagtgtac gcgccatcgc cgacttccac cggcgccacc agcatcgtgt
     5461 cgacgccgat cttcacgtcg gagccgatct tcgtgcggtg cttctcggcg ccgtcgtagt
     5521 tggcggtgat cgtgccggcc ccgagattcg agttttcacc gatctcggtg tcaccgatgt
     5581 aggacagatg cgggaccttc gagttcttgc cgatcgtcga gttcttgatc tcgacgaagg
     5641 tgcctgcctt ggctccgtcc tcgagcttcg ccccggggcg caggtaggcg aagggaccga
     5701 ccgagcaacc ctccgccagt tcgcagaggt tcagatagga ctgaatcacc tggcaaccct
     5761 tgccgatccc cgaatcgatg atcgtcgaat gcgggccgat cactgcctct tcggcgatct
     5821 cggtcgcgcc gcgcagggag gtgccaggct cgatccggga gtcctgcccg atcgtcaccc
     5881 cggcatcgat ccaggtcgtg gcggggtcga cgatcgttac gccgtcaagc atgtgccggc
     5941 ggaggatccg ctgacgggcc tcggcctcga ccacggccag gtcggcccgg ttgttgaccc
     6001 cgaggttgat cgccggatcc ttcgagacat gggccgtgac cagtttcccg cgggagcgca
     6061 tcaccgcgat cacgtcggtc aggtagtact cgccctgggc gttgtcgctg gagagctcac
     6121 cgagggccga agcaagcgat tcgccatcga agacataggt cccggagttg atctcgcgga
     6181 tcgccagctc ctcgcgggtg
//
LOCUS       DAOQKN010000149         6075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_6680_length_6075_cov_4.962791, whole genome shotgun sequence.
ACCESSION   DAOQKN010000149 DAOQKN010000000
VERSION     DAOQKN010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 6075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6075
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6680_length_6075_cov_4.962791"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1564
                     /locus_tag="PLJ64_09560"
     CDS             <1..1564
                     /locus_tag="PLJ64_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93770.1"
                     /translation="SPTITVDLRQGGNPVSVTNSPGSVTDNIGYSDSASQPWNRGSVW
                     GDNRLCTTTSMACNSGNTFNFSATCSNGTKSLSQTFTCPMGTPPGDGTFYQCIAGFDG
                     AVLDTYPSLIADNVAGQPNTFTNDDPTGIGNTPANTIYPQTFNAGTGNYGIGCDSVVI
                     DTAAPNTSITGGPAEGSSSADTTPTFTFTSTEAGGSFQCKVDGGSYATCSSGYTPTLG
                     TGGHIFSVRAIDAAGNTDPSPATRGFTITGPPDTTPPDTSITAGPAEGSTTDDSTPEF
                     SFTSTEAGSSFKCRVDSAPFATCTSPHTTATLSDGSHTFQVYATDSAGNADPSQASRS
                     FTVATGTPDTTPPETTITTNLPGGQTSDTTPTFEFTSSENGSSFECQVDSGTFTSCTS
                     PRTTGTLALGSHTFSVRATDPSGNTDPTPASKSFTVVNQPGDTTPPRTTIRTKPKKST
                     TSRKAKFTFTSNEAGSKFTCKLDKGPYRSCTSPFSKTVKPGKHSFSVKAKDGAGNTDR
                     SPATYTWKVRKP"
     gene            complement(1569..2330)
                     /locus_tag="PLJ64_09565"
     CDS             complement(1569..2330)
                     /locus_tag="PLJ64_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC93771.1"
                     /translation="MADKLEGAVVLVTGASSGIGEATARALAAQGAKVALVARRVDRI
                     EKLAQEIAAGGHTALAVEADVTDQEQAIAAVERTVGDFGRLDIVVNNAGVMLLGPIAG
                     APTEEWDRMIDVNIKGLLYTTHAALPHLFAAKDDSPRGVADVVNISSVAGRVASAGAG
                     VYNLTKHGVGAVSEALRQEASRHGVRVTIIEPGFVETELQSHNRSEVRERLEERISKI
                     EPLNPHDIADAIEYAVTRPAHVSLNELLIRPTTQP"
     gene            2391..3344
                     /locus_tag="PLJ64_09570"
     CDS             2391..3344
                     /locus_tag="PLJ64_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HNC93772.1"
                     /translation="MVATLPKSASSVRVAVIDIGTNSTRLLIADVDGSEVDEIYRESR
                     VTRLGRGVDHSGQLSAEGIEAVCNAIADYMPLIAGEEAGEVMVIATSAVRDAENGQAF
                     AAELRERFDLHCQIIDGDQEANYTYLGTSSGRNRDETMLVVDIGGGSTEMIIGRGPDP
                     VFHTSLQAGVVRHTERILTGDPPSSVELEALAKDIGKLVQASLEGVELPAVDRAVAVA
                     GTPSSLAAIDLELEPYDPKAVEGHRLPLRTVQWWLSRLASMPLEDRKQVPGLHPDRAQ
                     AIVAGVVILIEIMRNFDLLEIEVSEHDILYGCAITAAASAA"
     gene            complement(3372..4778)
                     /locus_tag="PLJ64_09575"
     CDS             complement(3372..4778)
                     /locus_tag="PLJ64_09575"
                     /inference="COORDINATES: protein motif:HMM:NF021378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HNC93773.1"
                     /translation="MTKERPHLGWVRFITILAGFITVLAILSTWVDRQVFDTQEWGDT
                     SLKLLQNPEIQKQVATYAVDELYANVDVQGELEKNLNDINKNLGDTLAGPLAGAARQG
                     ADSLALRALSNDKVQSAWKSANVAAHTTLINILEDKGTYTSTAGGQVELKLQPLIIEI
                     ADQVGLGDQARDKVPDSVGNIEIVDSDQLAQVQTVAKLIHGLALVTALLALVLIVLAV
                     YLSPGYRWLTLLWLAVALVLAALIILIIRSVAGNVLIPDLADPDIQPAAQAAWDIITE
                     LLKSVAWTVIWFAVALLVVAWVISPVKAAGKVREFLAVPFGQYPIPVFSLLGLAAFIF
                     LIMGAGDGREFIVRLAVVLMAGAGFWLFRRQVMLEYPDADFASLREFGERTRDRAKGA
                     WADRPKNISMPKIGGKDGDAAGQSGGEAETAVLPAQPATGEGSRLDQLEKLGKLKEAG
                     VLDDEEFAAEKKRILGGE"
     gene            complement(4832..4916)
                     /locus_tag="PLJ64_09580"
     tRNA            complement(4832..4916)
                     /locus_tag="PLJ64_09580"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4881..4883),aa:Leu,seq:taa)
     gene            5094..>6075
                     /locus_tag="PLJ64_09585"
     CDS             5094..>6075
                     /locus_tag="PLJ64_09585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNC93774.1"
                     /translation="MEASALNHAARRGLLARKSPLLKLQGDEKLVAMVRAGNSGAFDT
                     IVDRYQARLLGFCRQMIGSTEDAEDVLQEVFVNAYKAMMADNREINLRPWLYRIARNR
                     SLNHLRKPRAVAQESMDMVPMVDAASTAEKVHNREEFRQLLQDVTKLPETQRAALLLR
                     EIDALSYEEIAEAMDTTVPSVKSLLVRARISLAEASQARQLTCGEVRVELAEAAEGLA
                     KASGPVRRHVRDCEECAAFRKQLRTDNKALAAMMPIGVLAGLKATIAAKLGIGGSSAG
                     AGAGAAGTSAGAGAAGAAGAAGTGAAISAGTAASGIGAVTSAVGAKALAGV"
BASE COUNT         1099 a         2135 c         1848 g          993 t
ORIGIN      
        1 ttccccgacg atcacggtcg acctcagaca aggcggaaat cccgtgtcgg ttacgaactc
       61 gccgggttcg gtgacggaca acatcggcta ctcggattcc gccagccagc cctggaaccg
      121 gggatcggtc tggggtgaca accgcctttg cacgaccacg agcatggcct gcaattccgg
      181 aaacaccttc aacttctcgg ccacctgttc gaacggcacc aagtcactca gccagacctt
      241 cacctgcccg atgggtaccc cacccggaga cggaaccttc tatcaatgca ttgccggttt
      301 cgatggggct gtcctcgaca cctacccgtc gctaatcgcg gacaacgtgg ccggtcagcc
      361 caacacattc accaacgacg acccgaccgg gatcggcaat accccggcca acacgatcta
      421 tccccagact ttcaacgccg gcaccggcaa ctacgggatc ggctgcgaca gcgtcgtgat
      481 cgacaccgcc gccccgaaca cctcgatcac cggcggcccg gccgaaggct cgagcagcgc
      541 cgacaccacc ccgaccttca ccttcacctc gaccgaagca ggtggcagct tccaatgcaa
      601 ggtcgacggc ggcagctacg cgacctgctc aagcggatac acacccacgc tgggcaccgg
      661 tggccacatt ttctcggtgc gggcgatcga cgccgccggc aataccgacc cctcccccgc
      721 cacccgcggc ttcacgatca ccgggccgcc cgacaccacg ccgccggaca catcgatcac
      781 cgccggaccg gccgagggat cgaccacaga cgattcgacc cccgagttct cgttcacctc
      841 aaccgaggcc ggcagcagct tcaagtgccg ggttgacagc gccccgttcg ccacctgcac
      901 cagcccgcac accacggcga cgctgagcga cgggtcgcac accttccagg tctacgcgac
      961 cgattcggcg ggcaacgccg acccgagcca ggcttcccgc agcttcacgg tggccacggg
     1021 cactccggac acgactccgc ccgagaccac gatcacgacc aatctgccgg ggggccagac
     1081 aagcgacacc accccgactt tcgagttcac ctccagtgaa aacggcagca gcttcgaatg
     1141 ccaggtcgac tcgggcacct tcacctcgtg caccagcccg cgcaccacgg gaacgctcgc
     1201 cctcggctcc cacaccttca gcgtgagggc gaccgatccg tccgggaata ccgaccccac
     1261 gccagcgagc aagtccttca cggtcgtcaa ccagcccggc gacaccactc cgccccggac
     1321 gacgatcagg acgaagccga agaaatcaac gaccagccgg aaagcgaagt tcaccttcac
     1381 ctcgaacgag gccggttcga agttcacctg caagctggac aagggtccct acaggagctg
     1441 cacctcgccc ttctcgaaga cagtgaagcc cggcaaacac agcttctcgg tcaaggcgaa
     1501 ggacggcgcc ggcaacaccg atcgcagccc ggccacctac acctggaagg tcaggaagcc
     1561 ctagagcttc agggctgcgt ggttggccgg atcaagagct cgttcaggga gacgtgggca
     1621 ggacgggtga ccgcgtactc gatcgcgtcg gcgatgtcgt ggggattcaa cggctcgatc
     1681 ttcgagatcc gttcctcgag gcgctctcgt acttccgacc ggttgtggct ctggagttcc
     1741 gtctcgacga agcccggctc gatgatcgtg acccgcaccc cgtgccggct tgcttcctga
     1801 cgcagcgcct cgctgaccgc tccgacaccg tgcttggtca ggttgtagac cccggccccg
     1861 gcggatgcga cacggccggc gaccgaactg atgttgacca catccgccac gccgcggggt
     1921 gaatcgtcct tcgccgcgaa gaggtgcggc agcgccgcgt gggtcgtgta gagcaggccc
     1981 ttgatgttga cgtcgatcat ccggtcccac tcctcggtcg gggcgccggc gatcggcccg
     2041 agcagcatca ctcccgcgtt gttgacgacg atgtcgagcc ggccgaaatc tccgaccgtc
     2101 cgctcgaccg ccgcgatcgc ctgctcctga tcggtcacgt ccgcctcgac cgccagtgcg
     2161 gtgtggccgc cggccgcgat ctcctgggcg agcttttcga tccggtcaac gcgccgggcg
     2221 accagggcca ccttcgcacc ctgggcggcc agggcccggg cggtcgcctc gccgatgccg
     2281 ctgctcgctc cggtcaccag aaccactgcg ccttcaagtt tgtctgccat gcattcctct
     2341 tctcgtttgc cgtcaacccc gagattactc ccggggaata tccttgaacc ttggtcgcca
     2401 cccttcccaa gagcgcgtct tcggttcgcg tcgcggtaat cgacatcggc accaactcga
     2461 cgcggctgct gatcgccgac gtcgatggtt ccgaagtgga tgagatttac cgggaaagcc
     2521 gggtcacccg ccttggtcgc ggggtcgacc attccgggca gctttcggcc gaagggatcg
     2581 aagcggtctg caacgcgatc gccgactaca tgccgctgat cgccggggaa gaagccggcg
     2641 aggtcatggt gatcgcgacc agcgccgtcc gggatgcgga gaacggccag gccttcgccg
     2701 ccgagctcag ggaacggttc gacctccact gccagatcat cgacggtgac caggaggcga
     2761 actacaccta cctgggtact tcctccggcc gcaaccggga cgagaccatg ctggtcgtcg
     2821 acatcggtgg cggatcgacc gagatgatca tcggccgggg tcccgatccg gtctttcaca
     2881 cttcacttca ggccggcgtg gttcgccaca ccgaacggat cctgaccggt gacccgccca
     2941 gttcggtcga actagaagct ctggccaagg acatcggcaa gctggtccag gcatcgcttg
     3001 aaggagtcga gttgccggcg gtcgaccgcg ccgtcgccgt cgccggcact ccctcgtccc
     3061 tggccgcgat cgatctcgag ctcgagcctt acgacccgaa agcggtcgaa ggtcaccggc
     3121 ttccgctcag aaccgtccag tggtggcttt cccgcctcgc ctcgatgccg ctggaggatc
     3181 gcaagcaggt gccggggctt cacccggacc gtgcccaggc gatcgtcgcc ggggtggtga
     3241 tcctgatcga gatcatgcgc aacttcgacc tgctggagat cgaggtctcc gagcacgaca
     3301 tcctctacgg ctgcgcgatc accgcagccg cctcggccgc ctgaacccgg tcggcggccc
     3361 gacgccgccg ctcactcccc gccgaggatc cgcttcttct cggccgcgaa ctcctcgtca
     3421 tcgagcacac cggcttcctt gagcttgccg agcttttcga gctggtcgag ccggctgcct
     3481 tcccccgtgg caggctgggc gggcaggacc gcggtctcgg cctcaccacc gctctgaccg
     3541 gcggcatccc cgtcctttcc gccgatcttc ggcatcgaga tgttcttcgg ccggtcggcc
     3601 caggctccct tcgcccggtc acgggtccgc tctccgaatt cgcgcaggga agcgaagtcc
     3661 gcatcggggt actccagcat cacctgacgc cggaagagcc agaaaccggc tccggccatc
     3721 agcaccacgg ccagccggac gatgaactcc cggccgtcac ccgcacccat gatcaggaaa
     3781 atgaaggcag ccaggccgag caggctgaag accggaatcg gatactggcc gaagggcacc
     3841 gccagaaact cacggacctt cccggccgcc ttgaccgggg aaatgaccca ggcgacgacc
     3901 agcagggcca ccgcgaacca gatcacggtc caggccaccg acttcagcaa ctcggtgatg
     3961 atgtcccagg cggcctgtgc ggccggctgg atatccgggt cggccaggtc ggggatcagc
     4021 acgttgcccg ccaccgaacg gatgatcagg atgatcaggg ccgcaaggac cagcgcgacg
     4081 gccagccaga gcagggtcag ccagcggtat cccggcgaga ggtagacggc cagcacgatc
     4141 agcaccagcg caagcagcgc ggtgaccagg gcgaggccgt ggatcaactt cgccacggtc
     4201 tggacctggg ccagctggtc cgaatcgacg atctcgatgt tgccgaccga atcggggacc
     4261 ttgtccctcg cctggtcacc gaggccgacc tggtcggcga tctcgatgat cagcggctga
     4321 agtttgagtt cgacctgccc accggcggtg ctggtgtagg tgcccttgtc ctcgaggatg
     4381 ttgatcaggg tcgtatgcgc cgcgacattg gccgacttcc aggccgactg caccttgtcg
     4441 ttgctcagcg ctctcagggc gaggctgtcg gcgccctgcc tggccgctcc ggccagcggt
     4501 ccggccaggg tgtcgccaag attcttgttg atgtcattga ggttcttttc cagctcaccc
     4561 tggacgtcga cgttggcgta gagctcatcg accgcgtagg tcgcgacctg cttctggatc
     4621 tccgggttct gcagcagctt cagcgaggtg tcgccccact cctgggtgtc gaagacctgg
     4681 cggtcgaccc aggtcgaaag gatcgccagc acggtaatga aaccggccag gatcgtgatg
     4741 aacctgaccc acccgagatg cggccgctct ttggtcaaag cactactccc cttctgatct
     4801 gacgcgccaa tatgccgcaa gcctagccaa gtacccggag cgggactcga acccgcacgc
     4861 cccttgcggg actgcggctt ttaaggccgc tgcgtctacc attccgccac ccgggcccct
     4921 taagcctaga acttagggtt gtgggcgggg aacaggggta gaatgggagg caggcccaat
     4981 ctttagaaac cggaatattc ccgtttagag accgctacat ttggctgggt acggtgtttc
     5041 cgtaagcaca ggaggaaacc cggaccgcaa ggtccccagt caaaaggagt cccttggaag
     5101 catcagcact aaatcacgcg gcacgacgag gtcttctcgc caggaagtcg cccctgctga
     5161 agcttcaagg cgacgaaaag ctggtcgcca tggtgcgggc cggcaactcg ggagccttcg
     5221 acacgattgt tgatcggtac caggcccgtc tgctcggctt ctgccgccag atgatcggtt
     5281 cgaccgagga cgccgaggat gtcctgcagg aagtcttcgt caacgcctac aaggcgatga
     5341 tggccgacaa ccgggagatc aacctgcggc cctggctgta ccggatcgcc cgtaaccgct
     5401 ctttgaacca tctgcgcaag ccgcgcgccg tcgcccagga gtcgatggac atggtcccga
     5461 tggtggacgc cgcctcgacc gcggagaagg tccacaaccg cgaggagttc cgtcagctct
     5521 tgcaggacgt gaccaagctc cccgagaccc agcgcgccgc cctgcttctg cgcgagatcg
     5581 acgccctctc ctacgaggag atcgccgaag cgatggacac gaccgttccc tcggtcaagt
     5641 cgcttctggt ccgggcccgc atctccctgg ccgaagcgag ccaggcccgt cagctgacct
     5701 gtggtgaagt ccgggtcgag ctggccgagg ccgccgaagg cctcgccaag gcttccggcc
     5761 cggtccgccg gcacgtgcgc gactgcgagg aatgcgccgc cttccgcaag cagcttcgaa
     5821 ctgacaacaa ggctctcgcc gcgatgatgc cgatcggcgt gctggccggc ctcaaggcca
     5881 cgatcgccgc caagctcgga atcggtggat cctccgccgg tgcgggtgcc ggtgcagccg
     5941 gtacttccgc tggcgccggt gctgccggtg ccgctggtgc cgccggtacc ggtgccgcga
     6001 tcagcgccgg caccgccgcc tccggaatcg gtgccgtaac cagcgcggtc ggcgccaagg
     6061 ccctggccgg tgtcg
//
LOCUS       DAOQKN010000150         6051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_6727_length_6051_cov_5.522682, whole genome shotgun sequence.
ACCESSION   DAOQKN010000150 DAOQKN010000000
VERSION     DAOQKN010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 6051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6051
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6727_length_6051_cov_5.522682"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PLJ64_09590"
     CDS             <1..226
                     /locus_tag="PLJ64_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93775.1"
                     /translation="PQTAPRPRQKRSFIRSFFRFVLGILALVLIAAVVAGSVILLTDR
                     AASVKIKEWTGNSVEKISNELKQQIRDNTQ"
     gene            complement(227..952)
                     /locus_tag="PLJ64_09595"
     CDS             complement(227..952)
                     /locus_tag="PLJ64_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93776.1"
                     /translation="MRAELLKLRSMPTPRWTLAILLAFFVGGIVASVIWGVGTDSAVL
                     DLAVGLPGQIAALVVGSWMAGVEFGQNTLRRALSADPRRLRLVLSKLAVLVLAVVAVT
                     VILIVLGTLIFSLAGSGHATSIEIGTAGKILGAWLVADISWAVTAFSLTLLTRSMAGG
                     LVLSFVFFFVLDGFISLIPKVGDYTIGIALSDIDIAIRGTTDDFFGQTTTNDTGIAVI
                     VLVGWLAVFIAAGIVRTLKTEVK"
     gene            complement(942..1877)
                     /locus_tag="PLJ64_09600"
     CDS             complement(942..1877)
                     /locus_tag="PLJ64_09600"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC93777.1"
                     /translation="MPETLIRLEGVTKKFGATVALDDLSLAVPEGSVCGLLGPNGSGK
                     TTAIRILLGLAGMNSGTASLLGARPGGPGFTEAVRQTGSLIEGPALYGRATARQNMQI
                     QAATLGLADAGAEIDDLLELVDLGDRADSNARTFSLGMKQRLGLAISLLGKPRLVLLD
                     EPTNGLDPSGIVEIRELIKQLPERGVTVLVSSHLLSEVQLMCDRVTIINKGRLVADGT
                     MEEILASTGVGEGYRVYVRISQAEAATAILRRAGLEVEDRGEGSLVARGEIEDDSEIT
                     RILAESGIWLRGLMRERPDLERVFLSLTDGAGDES"
     gene            complement(1891..3096)
                     /gene="tgt"
                     /locus_tag="PLJ64_09605"
     CDS             complement(1891..3096)
                     /gene="tgt"
                     /locus_tag="PLJ64_09605"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNC93778.1"
                     /translation="MRHDPARLTFEIHARDGDARRGTLLTPHGPVETPAFIPLATKGS
                     VRGMSTREIEGMGYQMVLGNTYHLLVAPGPERIAEAGGLHRFMGWEKAIITDSGGFQV
                     FSLAHGGVAEEIKGSGRKMYGLAKTLGIEEEGVRFRSYKDGSELMLSPEESMRVQAAL
                     ASDIALVFDECTPFHADREYTARSTERTHRWLKRCLDWHEANGPAGQAVFGIIQGGTH
                     ADLRRESCEAVSSSAVDGISIGGTLGRDKEEMAEVLGFTVPNLPVEAPKHLLGIGEVD
                     DLMRGVALGLDVFDCAVPTRLARHGMALAPLPEKRFRIDVRKPREIGNREPLVPGCPC
                     AACERYDRDYLNYISKSEELTAVRLLVDHNLTYMERLMRGAREAISAGTYAAYSERIL
                     GGLAPWEAG"
     gene            complement(3176..4699)
                     /gene="gpmI"
                     /locus_tag="PLJ64_09610"
     CDS             complement(3176..4699)
                     /gene="gpmI"
                     /locus_tag="PLJ64_09610"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNC93779.1"
                     /translation="MANLPVPSAALIILDGWGIAPPGPGNAISLANTPNFDRLWEQYP
                     TTQLSAQGPDVGLPEGQMGNSEVGHLNLGAGAIVKQDLARIDTAVADGSFFDNEALVA
                     ACQKAVNGNGRLHLIGLVSDGGVHSGWEHLEACVELTSQEGVPDVIFHAITDGRDTLP
                     HGGAKYVEELERWLRSAGRVGTVSGRYYAMDRDTRWDRVKLAYDAIVHGAGPAAATAG
                     EAVADSYEHDLTDEFIKPTVIAGYDGAREGDTAIFINFRPDRARELTRALAEPDFDEF
                     VRSGGPLLDLTTMTSYREGWPYPVAFPEERPKVTMAQVIAGDGGRQLHVAETEKYPHV
                     TYFFNGGREREWEGEDRRLVESPRDVPTYDFKPEMSANQAADAFVAGWEEDEPRFGII
                     NFANPDMVGHTGVIPAAVQAIEAVDACLGRVVKTVLDSGGACLITADHGNADNMLEPD
                     GSPNTAHSLNPVPVIVTAEGFNLADGGILADVAPTILQMLGIQQPVEMTGRSLIEPA"
     gene            complement(4714..5472)
                     /gene="tpiA"
                     /locus_tag="PLJ64_09615"
     CDS             complement(4714..5472)
                     /gene="tpiA"
                     /locus_tag="PLJ64_09615"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNC93780.1"
                     /translation="MSRRPYVAANWKMNKTASEADEFLDAFLPTRSADGAEVVLCPPW
                     LALQRVAERCAGTPVKVCAQNMFYEDSGAFTGEVSAPMLLDIGVDGTILGHSERRQFF
                     GETDEALARKVPRAIEAGLEPILCVGETQAERESNDTESVLSHQVLTDLAEIHTDDLE
                     KVVIAYEPVWAIGTGLTASPEQAQDACEFIRNLLRSRDPHKAELVRILYGGSVKPANA
                     EELLALPDIDGALVGGAALDPDDFGAIVAAAPAA"
     gene            complement(5493..>6051)
                     /gene="pgk"
                     /locus_tag="PLJ64_09620"
     CDS             complement(5493..>6051)
                     /gene="pgk"
                     /locus_tag="PLJ64_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNC93781.1"
                     /translation="LEIADQILIGGAMCFPFYKAQGHAIGGSLCHDDDIPLAREALEK
                     AAAGNCELVLPTDLALGREFSADTDTEELDGVDVPEGWMGLDIGPKSAADYAARVGSA
                     GTVLWNGPMGAFELEPFAGGTRTVAEAVAAAPGYTVVGGGDSVAALVKFGLTDQVNWV
                     STGGGASLELLEGKDLPGVAALNDK"
BASE COUNT          989 a         2021 c         2041 g         1000 t
ORIGIN      
        1 cccccagacc gctccccggc cccgccagaa gcgttcgttc attcgctctt tcttccgctt
       61 cgtgctgggc atcctcgccc tggtcctgat cgccgcggtg gtcgcgggtt cagtcatcct
      121 gctgaccgac cgggcagcct cggtcaagat caaggaatgg accgggaatt cggtcgagaa
      181 gatctcgaac gagctgaagc agcagatccg cgacaacacc cagtagtcat ttcacttcgg
      241 tcttgagggt gcggacgatt cccgccgcga tgaagaccgc cagccagccg accaggacga
      301 ttaccgcgat cccggtgtca ttggtcgtgg tttgaccgaa gaagtcatcg gtggtgccgc
      361 ggatcgcgat gtcgatgtca gacagggcga tgccgatcgt gtagtccccg accttcggaa
      421 tcagtgaaat gaagccgtcg aggacgaaga agaagacgaa ggaaaggacc aggcccccgg
      481 ccatcgaccg ggtgaggagg gtaagcgaga aggcggtcac cgcccaggaa atgtcggcga
      541 ccagccaggc gccgagaatc ttccccgcgg tgccgatctc gatcgaggtg gcatgccccg
      601 agccggccag actgaaaatc agggttccga ggacgatcag gattaccgtc accgcgacca
      661 cggccaggac gagcacggcc agcttcgaaa gcaccaggcg aagccgccgc ggatcagccg
      721 agagcgcccg ccgaagcgtg ttctgcccga actccacccc ggccatccag gagccgacga
      781 ccagcgccgc gatctggccc ggaagcccaa ccgcaaggtc gagtacggca ctgtcggtcc
      841 ccacgcccca gatcaccgaa gcaacgatgc cgccgacgaa gaaggcgagc aggatcgcca
      901 gcgtccagcg cggtgtcggc atcgaacgca gcttgagcag ctcagctctc atcgccggct
      961 ccatcggtca gcgagaggaa gaccctctcg aggtcgggac gctcgcgcat cagcccgcgc
     1021 agccagatgc cggattcggc gaggatgcgg gtgatctccg agtcgtcctc gatctcgccc
     1081 cgggcgacga ggctgccctc tcccctgtcc tcgacttcca gacccgcccg gcggaggatc
     1141 gcggtcgccg cctcggcctg cgagatccgc acgtaaaccc tgtagccctc gccgaccccg
     1201 gtcgaggcga ggatctcctc catggttccg tcagcaacca gcctgccctt gttgatgatc
     1261 gtgacccggt cgcacatcag ctggacctcg gagagcagat gggaagagac cagcacggtc
     1321 acgccgcgct cgggaagttg cttgatcaat tccctgatct cgacgatccc cgaagggtcg
     1381 agaccgttgg tcggctcatc gagcaggacc agccggggct tgccgagcag ggagatcgcc
     1441 agaccgaggc gctgcttcat gcccagcgaa aaggtccggg cgttggagtc ggcccgatcg
     1501 cccaggtcga ccagttccag caggtcgtcg atctcggccc cggcatccgc gagcccgagc
     1561 gtcgcggcct ggatctgcat gttctgccgg gcggtggcac ggccgtagag cgccgggccc
     1621 tcgatcagcg agcccgtctg gcgaaccgct tcggtgaaac cagggccgcc gggccgggct
     1681 ccgagcaggc tcgcggttcc gctgttcatc ccggccaggc cgagcaggat cctgatcgcg
     1741 gtcgtcttgc cggaaccgtt tggaccgagc agcccgcaga ccgacccctc gggcaccgca
     1801 agggagaggt cgtccagggc gacggtggcc ccgaacttct tggtcactcc ttccagccgg
     1861 attagcgtct cgggcatcgc gggcggaatc ctaaccggct tcccagggcg ccagaccgcc
     1921 caggatccgc tcactgtagg cagcgtaggt tccggccgaa atcgcctcgc gggcaccacg
     1981 catcagccgt tccatgtagg tcaggttgtg gtcgaccagc agccggacgg cggtcagctc
     2041 ttccgacttc gagatgtagt tgaggtagtc ccggtcgtag cgctcacagg ccgcgcaggg
     2101 gcagccgggg accagcggct cgcggttgcc gatctcgcgt ggcttgcgga catcgatccg
     2161 gaagcgcttc tccggcagcg gcgccagggc catgccgtgg cgggcgagcc gggtcggaac
     2221 ggcacagtcg aagacatcga ggccgagcgc cacgcctcgc atcaggtcat caacttcgcc
     2281 gatcccgagc aggtgcttgg gagcctcgac cggcaggttc ggcaccgtga aaccgagcac
     2341 ttcggccatc tcttccttgt cgcggccgag ggtgccgccg atcgagatcc cgtcaaccgc
     2401 agatgaggag accgcctcgc aggactccct gcgcaggtcg gcgtgggtgc ctccctggat
     2461 gatcccgaag accgcctgcc cggccgggcc attggcttcg tgccagtcga ggcagcgttt
     2521 cagccagcgg tgcgtccgct cggtcgagcg ggcggtgtac tcacggtcgg cgtggaaggg
     2581 ggtgcattca tcgaagacca gggcgatgtc ggaagcgagc gcggcctgga cccgcatcga
     2641 ctcctccggt gaaagcatca actcggagcc gtccttgtag gaacggaatc gcactccctc
     2701 ctcctcgatc cccagggttt tggcgagccc gtacatcttg cgtcccgagc ccttgatctc
     2761 ctcggccacc ccgccgtggg ccagcgagaa gacctggaag ccgcccgagt cggtgatgat
     2821 cgccttctcc cagcccatga accggtggag cccgccggct tcggcgatcc gctccggccc
     2881 cggagcgacc agcaggtggt aggtgttgcc cagcaccatc tggtaaccca ttccctcgat
     2941 ttcccgcgtg gacatgccgc gaaccgagcc cttggttgcg agggggatga aggccggcgt
     3001 ttcgaccggg ccgtggggtg tcagaagggt gccgcgacgg gcatcgccat cgcgggcgtg
     3061 gatctcgaag gtcagccggg ccgggtcgtg gcgcatcagg ccaccctatc cgtccggaaa
     3121 accacgcgag attcccgttt gtgccggtat gcggcgcgaa ccgggatctc gcggatcagg
     3181 cgggctcgat caggctgcgg ccggtcatct ctaccggctg ctgaatcccg agcatctgga
     3241 ggatggtcgg tgcgacgtcg gcgaggatgc caccgtcggc caggttgaag ccctcggcgg
     3301 tgacgatgac cggcaccggg ttcagcgaat gagcggtgtt cggcgagccg tcgggttcaa
     3361 gcatgttgtc ggcgttgccg tgatcggctg tgatcaggca ggccccgccc gagtcgagga
     3421 cggtcttgac cacccggccg aggcaggcgt caaccgcctc gatcgcctgg accgcggcgg
     3481 ggatgacacc ggtgtgtccg accatgtccg ggttggcgaa gttgatgatc ccgaagcgcg
     3541 gttcgtcttc ctcccacccc gcgacgaagg catccgccgc ctggttggcg ctcatctcgg
     3601 gcttgaaatc gtaggtcggg acgtcgcggg gggattcgac caggcgccgg tcctccccct
     3661 cccattcgcg ctcgcggcct ccgttgaaga agtaggtcac gtggggatac ttctcggttt
     3721 ccgcgacatg gagctggcgg ccgccgtcgc cggcaatcac ctgggccata gtcaccttcg
     3781 gccgctcctc cgggaaggcg accgggtagg gccagccttc gcgataggag gtcatggtgg
     3841 tcaggtcgag tagcggtcca cccgagcgga cgaactcgtc gaagtccggt tcggccaggg
     3901 cgcgagtgag ttcccgggcc cggtcggggc ggaaattgat gaagatcgcg gtgtcaccct
     3961 cgcgggcccc gtcatagccg gcgatcacgg tcggcttgat gaactcgtcg gtcagatcat
     4021 gctcgtatga gtcggcgacc gcctcgcctg ccgtggcagc cgcgggacct gccccgtgga
     4081 cgatcgcgtc gtaggcgagt ttgacccggt cccagcgggt gtcgcggtcc atcgcgtagt
     4141 agcgaccgct gaccgtgccg acccgcccgg ccgagcgcag ccagcgttcc agttcctcga
     4201 cgtacttcgc gcccccgtgc ggcagggtgt cgcggccgtc ggtaatcgcg tggaagatca
     4261 cgtccggcac cccctcctgg gaggtcagtt cgacacaggc ctcaaggtgc tcccagccgg
     4321 aatgaacccc gccgtcggaa accaggccga tcagatgcag ccgcccgttg ccgttgaccg
     4381 ccttctggca ggccgcgacg agggcctcgt tgtcgaaaaa ggaaccgtcg gcgactgcgg
     4441 tgtcgatccg ggccaggtcc tgcttgacga tcgcaccggc gccgagattg aggtgaccga
     4501 cctcggagtt gcccatctgc ccttccggca ggccgacgtc cgggccctgg gcggagagct
     4561 gcgtggtcgg gtactgctcc cagagccggt cgaagttggg cgtgttcgcc agcgagatcg
     4621 cgttgcccgg gccgggcggg gcgatgcccc agccgtcgag gattatcagc gccgccgatg
     4681 gtacgggcag gttcgccatg ggtcccgggt tccttacgcg gccggcgcgg cggcgacgat
     4741 cgcgccgaag tcatccgggt cgagggcggc gccgccaacc agggcgccgt cgatgtcagg
     4801 cagcgccagg agctcttccg cgttggccgg tttgaccgat ccgccgtaaa ggatccgcac
     4861 cagctcggcc ttgtggggat cccgcgagcg cagcaggttg cgaatgaatt cgcaggcatc
     4921 ctgggcctgt tccggggaag cggtgaggcc ggtgccgatc gcccagacgg gctcgtaggc
     4981 gatcaccacc ttctccaggt cgtcggtgtg aatctccgcc aggtcggtca ggacctgatg
     5041 cgaaagcacc gattcggtgt cgttcgattc ccgctcggcc tgggtctcac cgacgcagag
     5101 gatcggctcg aggccggctt cgatcgccct cggaaccttg cgggccagcg cctcgtcggt
     5161 ctcgccgaag aactggcgcc gttcggagtg gccgaggatc gtgccgtcaa cgccgatgtc
     5221 aagcagcatc ggggccgaga cctcgccggt gaaggctccg gaatcctcgt agaacatgtt
     5281 ctgggcgcat accttgaccg gggtgccggc gcagcgctcg gccacccgct gaagagccag
     5341 ccacggcggg cagagcacga cctcggcccc gtcggcggac cgggtcggca ggaaggcatc
     5401 gaggaactcg tccgcttctg acgcggtctt gttcattttc cagttggcgg ctacatacgg
     5461 tcgacggctc atgtccggcc tctttcgatt tgttacttgt cgttcaatgc ggccacgccc
     5521 gggaggtcct tgccctccag cagttcaagc gaagcgccgc ctccggtcga aacccagttc
     5581 acctggtcgg tcagcccgaa cttgaccagg gcggcgaccg aatcgccgcc gccgaccacg
     5641 gtgtatccgg gagcggctgc caccgcctcg gccacggtcc gggtgccgcc ggcgaatggt
     5701 tccagctcga aggcccccat cggcccgttc cagagcacgg ttccggccga tccgacccgg
     5761 gctgcgtaat ccgccgcgga cttcggaccg atgtcgaggc ccatccatcc ttccggcacg
     5821 tccactccgt cgagttcctc ggtgtcggtg tcggccgaga actcgcggcc gagcgcgagg
     5881 tcggtcggca ggaccagctc gcaattgccg gcggcggcct tctccagggc ttcacgggcg
     5941 agcgggatgt cgtcgtcatg gcagagcgag ccaccgatcg cgtgcccctg ggccttgtag
     6001 aaggggaagc acatggcgcc gccgatcagg atctggtcgg cgatctcaag g
//
LOCUS       DAOQKN010000151         6016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_6794_length_6016_cov_6.750377, whole genome shotgun sequence.
ACCESSION   DAOQKN010000151 DAOQKN010000000
VERSION     DAOQKN010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 6016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6016
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6794_length_6016_cov_6.750377"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="PLJ64_09625"
     CDS             complement(<1..543)
                     /locus_tag="PLJ64_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93782.1"
                     /translation="MRSVRQAILLLFCLLAFAAAPAGGASANPAWKPHVNLARRWAGT
                     RSGEISFAVVTGGHRYMFHGLRMDPMASTVKVMLMTSYLRSRPVRRRNLHPGERRLIR
                     RMIRHSDDFAATEIRNRIGHSGLPHLARTVGIHAFIYSAIWGECRSNPRSQAGFMRRL
                     PDLLPRRHRRFALRQLNRITP"
     gene            complement(616..870)
                     /gene="rpmA"
                     /locus_tag="PLJ64_09630"
     CDS             complement(616..870)
                     /gene="rpmA"
                     /locus_tag="PLJ64_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNC93783.1"
                     /translation="MAHKKGLGSSRNGRDSNPKMLGVKVFAGETVSGGEIIVRQRGTK
                     FWPGEGTGLGRDHTIFATRPGTVEFKPGRRGRTISVAPAE"
     gene            complement(860..1402)
                     /gene="rplU"
                     /locus_tag="PLJ64_09635"
     CDS             complement(860..1402)
                     /gene="rplU"
                     /locus_tag="PLJ64_09635"
                     /inference="COORDINATES: protein motif:HMM:NF013025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNC93784.1"
                     /translation="MYAVVESGGKQYRVEKGTVLVVDRLKAEQGDKVALKPVMFKDKE
                     VIVDAKGLEKIKIEATVTEHLRGDKIKVFKYKPKKGYSRRAGHRAELTKLEVTSLGGG
                     AKKAAPKKAAADKAEEKPKAEAKAETAEKKAPAKKPAAKKPAAKAETKTETKAAAKKP
                     AAKKPAAKKPAAKKEDTDGA"
     gene            complement(1476..3506)
                     /locus_tag="PLJ64_09640"
     CDS             complement(1476..3506)
                     /locus_tag="PLJ64_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HNC93785.1"
                     /translation="MKKTIVVSADRGETRVAVLEAKTVKSKREVAEVYIERRGGRSLV
                     GNIYLGKVDNVLPGMEAAFVDIGLERNGFLHVDEILLPDGQQAPRRGRGQGRRIDELI
                     KPKQEILVQVIKDPIKSKGARLSMNVTIAGRYLVYAPNGTGVGVSRRLADKERDRLRR
                     MVSRTYDGPGGLIVRTAAHGAKKADFTREIAYLHRLDAVLEKRREGAKAPSLVFQEQD
                     LPVRVLRDVFLNEFDKAIIDDQKQFDRVTSFFQRTAPELVEGVEHYKGKKPLFEKWEI
                     ERAIQSTLGRRIDLPSGGYLIIDYTEALTVIDVNSGSFTGRGKGGLEETITKVNTEAA
                     SEVVRQLRLRDIGGIIVIDFIDMAKGKNRDKVLGTLREALDADKSKSYLVEISPLGLV
                     EMTRQNITDGVREILTVQCPTCEGEGVVLSAETVALEGLRKMKEIAADHAGDEAFLVR
                     VNPKVAALLNDSDSGLLELEEETGKQFHFEGGEALAIDNFELIESGEKSSIEERALPF
                     RPGEEVFVKIEEPHMYNADDAIARIDSYIISITGAANAVGERKMVRIQSVERASAVAE
                     LLPEEAQPKETKKAAPRKKNGSGQNAGRSENGKASASGRGPKQETGNRGNDRQGRSGQ
                     GSKNGSDPKPKAESAVKKDAEGEKEDKPVKSGPFGLRRGRRRRGKKKEDAGS"
     gene            complement(3550..4734)
                     /locus_tag="PLJ64_09645"
     CDS             complement(3550..4734)
                     /locus_tag="PLJ64_09645"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNC93786.1"
                     /translation="MESSRLPRRTPKQSVNPLERAGLAGMDGWLAFSALGLLACSLFT
                     LYEASGSQIVGAPEQYVQRQALYGLVGIIGMIAVSRIDYSRFRELRVGIYTFLCASIF
                     AVFTFGVAARGSTNSLELPFFTFQPAELGKLLLVLALAGFVIDGARRGSEGQRTIRIL
                     ALGLVPTGLVLLQDLGTSLVFAAITFAIMVAAGVRWSHLAAIGAVLAVFVTVVLVALP
                     AAGMPVLKEYQQERLTSFLSPSSDIGDAGYQQNQAKVGIGAGGTTGRGDQATQSRFGF
                     VPERHTDFIFAVVAERYGFVGAALVLSLFALLFWRALRLMTLSKNFYGTLVAAGIAAA
                     IIFQVFVNVGMNLGIMPITGIPLPLMSYGGSAVIATFLMIGVLQSIHIESRAAERGPW
                     ID"
     gene            complement(4734..>6016)
                     /locus_tag="PLJ64_09650"
     CDS             complement(4734..>6016)
                     /locus_tag="PLJ64_09650"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNC93787.1"
                     /translation="KDLAAGDTLGKTGIEQTYDDELRGQPGDTKVQVDSTGRIVSQLP
                     STLPVPGESVRLSIDGKVQQAGEASLQSIGLPGAFVAMDVNNGQVLGLASNPTFDPDM
                     FTHPLTQNQVDALYDEDNGAPLFDRAIGGQYAVGSTFKPFTALAALDGGYITPQTVVN
                     DTGSITIADQEFDNAGKQAHGPVDLQRALEVSSDVYFYMLGRDMNGTRQLQNWAGDFG
                     FGKATGIDIPGESEGLVPSPKWVNQAHKEEPKYYDPWAIGQNIQFAIGQGYFQASPLQ
                     LATAYAALGNGGTVLQPQLMLQTEDAAGRVKSEAEPVVTGEIPIDPAARQVIMDGLHD
                     AAQAPEGTSYKVFGGFPVKVAGKTGTAERTDEIDQAWYAVLAPYPDPEIAVVVTVEQG
                     GFGADTAAPVALQILQAYFDKQAHAVSGGVGSVE"
BASE COUNT          952 a         1940 c         1948 g         1176 t
ORIGIN      
        1 cggagtgatc cggttgagct gccgcagggc aaaccggcgg tgacgccgcg gcaaaaggtc
       61 cggaaggcgc ctcatgaaac cggcctggct gcgcgggttt gaccggcact cgccccatat
      121 cgccgagtag atgaaagcgt ggatgccgac cgtccgggcg aggtgcggca gtcccgagtg
      181 accgatccgg ttgcggatct cggtcgccgc gaagtcatcg gagtgccgga tcatccgcct
      241 gatcagccga cgctcaccgg ggtgaaggtt ccggcggcga accggccggc ttctcaggta
      301 tgaggtcatc agcatcacct tgaccgtgct ggccatcgga tccatgcgca gtccgtggaa
      361 catgtagcgg tggccgccgg tgaccacggc gaaggagatc tcgccgctcc gggtgccggc
      421 ccatcgccgg gcgaggttca catgcggctt ccaggccgga ttagcacttg cgcccccggc
      481 cggcgcggcg gcgaaggcga gcaggcaaaa gaggagcagg atcgcctggc gaacggaacg
      541 catccattca cggtaccccg gaaaatgcaa aagaaaaggc ccgcggagcg ggcctttccg
      601 aatcctgttt cccggttact cggccggagc gaccgagatc gtcctgccgc ggcggccggg
      661 cttgaactcg accgtgccgg gccgggtggc gaaaatggtg tggtcacggc cgaggccggt
      721 gccctcgccg ggccagaact tcgtgccgcg ctggcggacg atgatctcgc cgccggaaac
      781 ggtttctccg gcgaagacct tcaccccgag catcttcggg ttcgagtcac gaccgttccg
      841 tgaggatccg agtcctttct tatgcgccat cggtgtcctc cttcttggcg gccggcttct
      901 tggccgcagg ctttttggcg gccggcttct tcgcggcagc cttggtttca gtcttggttt
      961 cggccttggc cgccggcttc ttcgcggccg gcttctttgc cggggccttc ttctcggcgg
     1021 tttccgcctt ggcttcggcc ttcggcttct cctccgcctt gtcggcagca gccttcttcg
     1081 gggcggcctt cttcgcgccg ccacccaggg aagtcacctc gagcttcgtc aactcggccc
     1141 ggtgaccggc ccggcggctg tagcccttct tcggcttgta cttgaagacc ttgatcttgt
     1201 cgccgcgcag atgctcggtg acggtcgcct cgatcttgat cttctcgagg cctttcgcgt
     1261 caacgatgac ctccttgtcc ttgaacatga ccggcttcag ggcgaccttg tcaccctgct
     1321 cggccttcag gcgatcaacg acgagcaccg tgcccttttc gacccggtac tgcttgccac
     1381 cactttcaac taccgcgtac atgtcttgaa ctcaaatcct tgtctggtct tcctgaagct
     1441 gagtcttctc agcctgaaat cggcgggacc gaggatcaac taccggcgtc ttccttcttc
     1501 ttgccacgcc gacgtcgtcc gcgcctcagg ccgaacgggc cggattttac cggtttgtcc
     1561 tctttttcac cttctgcgtc ctttttcacc gctgattcgg ctttgggttt cgggtcggaa
     1621 ccgttcttcg agccctgacc ggacctgccc tggcgatcgt taccgcgatt cccggtctcc
     1681 tgtttcgggc cgcgacccga cgcggaagcc ttgccgtttt cgctcctgcc ggcattctgg
     1741 cccgacccgt tcttctttcg aggggcggcc ttcttcgtct ccttgggctg ggcttcctcc
     1801 ggcagcagct cggccaccgc ggaggcccgc tcgaccgact ggatgcggac catcttgcgc
     1861 tcaccgaccg cattcgcggc accggtgatg ctgatgatgt aggagtcgat ccgggcgatc
     1921 gcgtcatccg cgttgtacat gtgcggctct tcgatcttga cgaacacttc ctcgcccggc
     1981 cggaagggca gggccctttc ctcgatcgaa gacttctcgc cggactcgat cagttcgaag
     2041 ttgtcgatcg ccagggcctc gccgccctcg aagtggaact gcttgccggt ttcttcctcg
     2101 agctcgagca ggccggagtc ggaatcgttg agcagcgcgg cgaccttcgg gttgacccgg
     2161 accaggaagg cctcgtcacc ggcgtggtcg gccgcgatct ccttcatctt gcggagacct
     2221 tccagggcga ccgtctcggc cgagagcacc acgccctcgc cctcgcaggt cgggcactgg
     2281 acggtgagga tctcgcgcac cccgtcggtg atgttctggc gggtcatctc gaccaggccg
     2341 agcggcgaaa tctcgaccag gtagctcttc gacttgtcgg cgtcgagcgc ttcccgcagg
     2401 gtgccgagca ccttgtcgcg gttcttgccc ttcgccatgt cgatgaagtc gatgacgatg
     2461 atgccgccga tgtcgcgcag cctgagctgc cggacgactt cggaggccgc ctcggtgttg
     2521 accttggtga tcgtctcctc gaggccgccc tttccgcggc cggtgaagga accggagttc
     2581 acgtcgatca cggtcagcgc ctcggtgtag tcgatgatca ggtagccgcc ggagggcagg
     2641 tcgatacgac ggcccagggt cgactggatc gccctttcga tctcccattt ctcgaagagc
     2701 ggcttcttgc ccttgtaatg ctcgacccct tcgaccagct ccggcgcggt gcgctggaag
     2761 aagctggtta cccggtcgaa ctgtttctgg tcatcgatga tcgccttgtc gaactcgttg
     2821 aggaagacgt cgcggagcac gcggaccggc aggtcctgct cctggaagac gagggatggc
     2881 gccttggcgc cctcgcggcg cttctcgagc acggcgtcga ggcgatgcag gtaggcgatc
     2941 tcgcgggtga agtcggcctt tttggcgccg tgggccgcgg tgcggacgat caggccgccc
     3001 gggccgtcat aggttcggct gaccatgcgg cgcaggcggt cgcgttcctt gtcggcgagc
     3061 cggcggctca cccccacccc ggtgccgttc ggcgcgtaga ccaggtagcg gccggcgatg
     3121 gtcacgttca tcgaaaggcg ggcgcccttc gacttgatcg gatccttgat cacctggacc
     3181 aggatctcct gtttgggctt gatcagctcg tcgatccggc ggccctggcc gcggccgcgg
     3241 cggggtgcct gctggccgtc ggggagcagg atctcgtcga catgcaggaa gccgtttcgc
     3301 tccaggccga tgtcaacgaa ggccgcttcc atcccgggca ggacgttgtc gaccttgccc
     3361 aggtagatgt tcccgaccag ggagcggccg ccccggcgtt cgatgtagac ctcggccact
     3421 tcgcgctttg atttgaccgt cttggcctcg agcacggcaa cgcgggtttc gccgcggtcg
     3481 gccgacacaa caattgtctt tttcattttc taagtcgttt tccgttcaga ttggctgccc
     3541 tgtggattct cagtcgatcc acggacccct ctctgcggcg cgcgactcga tgtgaatgct
     3601 ctgcaggacc ccgatcatca ggaaggtggc gattaccgcc gacccgccgt aactcatcag
     3661 cggcaagggg atgccggtga tcggcatgat gccgaggttc attcccacgt ttacgaagac
     3721 ctgaaagatg atcgcggcag cgattccggc cgcgaccagg gttccgtaga aattcttcga
     3781 gagcgtcatc agacgcagcg cccgccagaa gagcagggca aaaagggata agaccagcgc
     3841 cgcgccaacg aatccgtaac gctcggcaac cacggcaaaa atgaagtccg tgtgccgttc
     3901 gggaacgaat ccgaagcggg actgggtggc ctggtcgccg cggccggtgg ttccaccggc
     3961 tccgattccg accttggcct gattctgctg gtagcccgcg tcaccgatat cggacgacgg
     4021 gctgaggaac gaggtaagtc gttcctgttg atactccttc agcaccggca tcccggccgc
     4081 cggaagggcg accagcacga cggtgacaaa aactgcgagg acggccccga tcgcggccag
     4141 gtgagaccag cgaacgccgg ccgcgaccat gatcgcgaag gtaatcgccg cgaagacgag
     4201 cgacgtaccc aggtcctgaa gcaggaccag gccggtcggc acgagtccga gggcgagtat
     4261 gcggatcgtc ctttgacctt cggagccacg acgggccccg tcgatcacga atccggccag
     4321 ggcaagcacg agaagcagct tgcccagctc ggccggctgg aaggtgaaga aaggaagttc
     4381 aagtgagttc gtcgacccgc gtgcggccac gccgaatgtg aacaccgcga agatcgaggc
     4441 gcacaggaac gtgtagatgc caacacgtag ttcccggaaa cgggaatagt cgatgcggga
     4501 gacggcaatc attccgatga ttccgaccaa accatagaga gcctgccgct ggacgtactg
     4561 ctcgggtgcc ccgacgatct gcgagccgga agcctcgtat agcgtgaaaa gcgagcaggc
     4621 gagcaggccc agagccgaaa aggcaagcca tccgtccatc ccggccaggc cggcgcgctc
     4681 cagcgggttg actgactgct tcggggtccg gcgcggcagc cgcgaggatt ccatcactcc
     4741 acgctcccga ccccgcccga gaccgcatgg gcctgtttgt cgaaataggc ctggaggatc
     4801 tggagggcga ccggcgcggc ggtgtcggca ccgaacccgc cttgctcgac cgtgacgaca
     4861 acggcgattt ccggatccgg atacggggcc agcaccgcgt accaggcctg gtcgatttca
     4921 tcggtgcgct ccgcggtgcc ggtcttgccc gcgaccttga ccgggaagcc gccgaaaacc
     4981 ttgtacgagg tgccttcggg tgcctgcgcg gcgtcgtgaa gtccgtccat gatcacctgc
     5041 cgggccgccg gatcaatcgg gatttccccg gtcacaaccg gttcggcctc ggacttgacc
     5101 cggcccgctg cgtcctcggt ctgcagcatc agctgcggct ggagaacggt gccgccgttg
     5161 ccgagcgcgg cgtaggccgt cgccagctgc agcggactgg cctggaagta gccctggccg
     5221 atcgcgaact ggatgttctg gccgatcgcc cacgggtcgt agtacttcgg ctcttccttg
     5281 tgggcctggt tcacccactt cgggctcggc accaggcctt ccgattcacc cggtatgtcg
     5341 atcccggtcg ccttgccgaa gccgaagtcg ccggcccagt tctggagctg gcgggtgccg
     5401 ttcatgtcgc ggccgagcat gtagaaatag acgtcggagg agacctcgag cgcgcgctga
     5461 aggtcgaccg gcccatgggc ctgtttgccg gcgttgtcga attcctgatc ggcgatggtg
     5521 atcgagccgg tgtcgttgac gacggtttgc ggggtgatgt agccgccatc gagtgcggcc
     5581 agggcggtga agggcttgaa ggtcgagccg accgcatact ggccgccgat cgcccggtca
     5641 aagagcggtg ccccgttgtc ctcgtcatag agcgcgtcga cctggttctg ggtcagcgga
     5701 tgggtgaaca tgtccgggtc gaaggtcggg ttggaagcga ggcccagcac ctgcccgttg
     5761 ttcacgtcca tggcgacgaa ggcgccgggc aacccgatgc tctgaagcga tgcctctcct
     5821 gcctgctgca ccttcccgtc gatcgaaagc cgtacgctct cgcccgggac cggcagggtc
     5881 gagggcagct ggctgacgat ccggccggtc gaatcgacct gcaccttcgt gtcccctggc
     5941 tggccgcgca gttcgtcgtc gtaggtctgt tcgatcccgg tcttgccgag cgtgtcaccg
     6001 gcggcgaggt ccttgt
//
LOCUS       DAOQKN010000152         6010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_6810_length_6010_cov_4.864148, whole genome shotgun sequence.
ACCESSION   DAOQKN010000152 DAOQKN010000000
VERSION     DAOQKN010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 6010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6010
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6810_length_6010_cov_4.864148"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..1430)
                     /locus_tag="PLJ64_09655"
     CDS             complement(24..1430)
                     /locus_tag="PLJ64_09655"
                     /inference="COORDINATES: protein motif:HMM:NF012946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNC93788.1"
                     /translation="MEASGVETGRQAEMNGAGPSGGGGPRVCVIGAGSSGITAMRSLQ
                     EKGIDFVCFEKGSWIGGNWKYDNDNGQSAAYKSLHINTSRQMMEYKAFPMPEDFPDYP
                     DHFQIASYFEDFVDHFGLRSSIRFQTEVESVVPAEGGGWEVTWQNREGESGTDHFSDV
                     MVCNGHHWDKRWPEPAFPGSDTFEGEQMHAHDYRTPEIFEGKRVLVLGIGNSATDIAV
                     EASRHADATFLSMRRGAWIVPKYFGSKPTDRATTGLMSRLPLKVQSAGLWLTLRLAVG
                     DPTDYGLPKPDHMPLHAHPTVSQDLLGRLGHGDIKVKKNIQRLDGDSVVFEDGSREQV
                     DLIVYCTGYKITFPFLAPEVFNAAENKVDLFQRVVPPDRPGLYFIGLIQPLGAIMPLA
                     EIQCDWVAELIDGEGALPDRLEMSREIAMDQAKMGRRFVSSKRHTVEVDFDPYVRALN
                     KERALSRERAAGSKLSVA"
     gene            1510..2904
                     /locus_tag="PLJ64_09660"
     CDS             1510..2904
                     /locus_tag="PLJ64_09660"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HNC93789.1"
                     /translation="MNEATGTAPGRERASRIAIVGGGASGTLVAANLLRMAEVPLEIE
                     LIDESGDSGPGIPYRTPNPEHRLNVPACRMSAWADDPDHYLNWRRSRDPEAAPEEYTA
                     RSIYGEYLQELLETAATKAPPFATLGRLTDRVAGISFADGRPVIRFPDRQLEVDQVVI
                     ATGPVPGGDPVPVPEALKDRGLYLPSAWDTEAVETAGKDEEVLIIGTGLTMVDVALTL
                     GAAPDGPRIHAISRSGMFPKTHLDRLTRLSTPQIPPEGPVSKRELLGAFAEQLSLASA
                     RGDDWRDAIDSMRLVTGDAWRRMSVEDRRWLLENVNRPWEVHRYRMAPSVGAAFHRLI
                     DSGRVEIQQARVGAIEESGSRVRVELVGPKRSESREYDRVISACGACTDIRRDAPEPV
                     RGLIDSGHLRPDDLLLGVEVDRDGAARDARGDRSATISVLGSLRRGVDWESIGVTELK
                     TQSRDIALRLLDQM"
     gene            complement(2895..3911)
                     /locus_tag="PLJ64_09665"
     CDS             complement(2895..3911)
                     /locus_tag="PLJ64_09665"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HNC93790.1"
                     /translation="MALAEPATSRETNFLIARYCGSQHGVVAMFQLTKAGVSKDAVVA
                     RARSGWLTAIHDGVYATSPRQLTVWGRAMAAVLAAGPDACLSHQSAAWLWKLDSARPV
                     IHVVKRGGMNRELKPFDGYGKVIVHSTRRLDPCEVTEVNGIPVTSFIRTTIDLAGGMT
                     REALDDYLADADRENLLRLKELERELDRWTGKKGITRLRRTLGDWHPSTEEEMLIFQK
                     EFVGALVRAGAPRPRVDPLLDGCLIDLLWEEYMLMFELDGHAFHRDPAMQTKDARRDR
                     KHTLMGYQVNRFTRQEFRDNRAEVIGQALQLLRSRGWRGPDPQPLHQGVYRSLEAPGP
                     SDHI"
     gene            complement(4273..>6010)
                     /locus_tag="PLJ64_09670"
     CDS             complement(4273..>6010)
                     /locus_tag="PLJ64_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNC93791.1"
                     /translation="NNVVPPPEKEEILAKYDEETARIQAEFDEGWHTAEERHKQVTDK
                     WNAATEEVGQAMEDNLRQLNPIFMMANSGARGSFKQIRQLAGMRGLMANPKGEIIERP
                     IKSNFIEGLSVGEYFISTHGARKGLADTALRTADSGYLTRRLVDVAQDVIVLEDDCKT
                     KESIDMPLFIDGEPNPNVVGRLSAKKFETKRGRELLKKNQLISKADAAELTDAYEGDE
                     SATVPVRSAFKCESEHGICRSCYGVSPATGFMVEIGDSIGTIAAQSIGEPGTQLTMRT
                     FHTGGVAGQDITQGLPRVVELFEARKPKGLAQMAEVAGKVGVEQTEKQITVTVLESNG
                     EESSYTFPPRTRLNVKQGDKVEVGDQLNEGSLYPADVLSIRGRTAVEQYLVAEVQRVY
                     KAQGVEINDKHIEVIVRQMLKKVEVETKGSTELLPGQLEDRQVLKAINKQVKADGGTA
                     AKAQEKILGITKASLATKSFLSAASFQETTKVLTDAALEGKRDRLVGLKENVIIGKLI
                     PAATGLKHYRSMTIEPTEPFAPAEEAVLLEEDLLVENGDGNGAGGADGFGESFADELD
                     EISQEIDASTEK"
BASE COUNT         1022 a         1971 c         1892 g         1125 t
ORIGIN      
        1 cacgagcccg atcgctcccg gaactaggcg accgacagct tcgagccggc ggcgcgttcg
       61 cgggaaagcg cccgctcctt gttcagcgcc cgcacgtagg ggtcgaagtc aacctcgacc
      121 gtgtggcgct tggaactcac gaaccggcga cccatcttcg cctgatccat cgcgatctcc
      181 cggctcatct cgagccggtc gggcagggcg ccctcgccgt cgatcaactc ggcgacccag
      241 tcgcactgga tctcggccag cggcatgatc gccccgagcg gctggatcag gccgatgaag
      301 tagaggccgg gccgatcggg cggaacgacc cgctggaaga ggtcgacctt gttctcggcc
      361 gcgttgaata cttccggcgc gaggaacggg aaggtgatct tgtagccggt gcagtaaacg
      421 atcaggtcaa cctgctcccg gctgccatcc tcgaagacga ccgagtcgcc gtcgaggcgc
      481 tggatgttct tcttgacctt gatgtccccg tgtccgagcc ggccgagcag gtcctgcgag
      541 acggtcgggt gagcgtggag gggcatgtgg tcgggtttgg gcaacccgta atcggtcggg
      601 tccccgaccg caaggcggag ggtcagccag agaccggcgc tctggacctt gagcggaagc
      661 ctggacatca ggccggtggt cgcccggtcg gtcggcttgc tgccgaagta cttgggaacg
      721 atccaggcgc cgcgcctcat cgagaggaag gttgcgtctg cgtggcggga ggcctcgacc
      781 gcgatgtcgg tcgcggagtt gccgatcccg agcaccagca cccgcttgcc ttcgaagatc
      841 tccggggtcc ggtagtcgtg ggcgtgcatc tgttcgccct cgaaggtgtc cgaaccggga
      901 aaggcgggtt ccggccagcg cttgtcccag tggtggccgt tgcagaccat tacatcgctg
      961 aaatggtcgg tgccgctctc gccctcacgg ttctgccagg tcacttccca gccgccccct
     1021 tcggcgggca cgaccgactc gacctcggtc tgaaagcgga tcgaactcct caggccgaaa
     1081 tggtcgacga aatcctcgaa gtaactggcg atctggaagt ggtccggata gtccgggaaa
     1141 tcctccggca tcgggaacgc cttgtactcc atcatctgcc gtgaagtgtt gatgtgaagc
     1201 gacttgtagg cggcagactg gccgttgtca ttgtcgtact tccagttgcc gccgatccag
     1261 gatcccttct cgaagcagac gaaatcaatc cccttctcct gaaggcttcg catcgcggtg
     1321 atgcccgagg acccggcgcc gatcacgcag acccgcggcc cgcccccgcc ggacgggccg
     1381 gctccgttca tctccgcctg tctgcccgtc tccactcctg aagcttccat tcgggcagtg
     1441 tagccaatac ttgacatggc ttcgtgtcag tttgccctca ctaggctcct gactgatggg
     1501 aaagtccgga tgaacgaagc aaccgggacc gcacccggcc gggagcgggc gtcccggatc
     1561 gccatcgtcg ggggcggcgc ttccggaact ctggtggccg cgaacctgct tcgaatggcc
     1621 gaggtgccgc tggagatcga actgattgac gaatccggcg attccggtcc tggcatcccc
     1681 taccggaccc ccaaccccga gcatcgtctc aacgtgcccg cctgccggat gagcgcgtgg
     1741 gcagacgatc cggaccatta cctgaactgg cgccggtccc gcgatcccga ggcggccccg
     1801 gaggaataca cggcccgatc catatacggc gagtacctgc aggaactgct cgaaactgcc
     1861 gcgacgaaag cccctccgtt cgccacactc ggccggctga ccgaccgggt tgccggcatc
     1921 tccttcgctg acggaaggcc cgtcattcgg ttccccgatc ggcagctcga ggtggatcaa
     1981 gtggtcatcg caaccgggcc ggtgcccggg ggcgacccgg ttccggtccc cgaagcgctg
     2041 aaagacaggg ggctctatct acccagtgcc tgggacaccg aggcggtcga aacagccggc
     2101 aaggacgaag aggtactgat catcggcacc gggctgacca tggtcgacgt cgcgctcact
     2161 ctcggcgccg cacccgacgg acctcgcatt cacgccatct cccgcagcgg catgttcccc
     2221 aagacccacc tggatcgcct gacccgcctt tccaccccgc agataccgcc cgaggggccg
     2281 gtgtcgaagc gcgaattgct gggggcgttt gccgagcaac tgtcgctggc ctcggcccgg
     2341 ggcgacgact ggcgcgacgc gatcgattca atgcgactgg tcaccggaga cgcctggcga
     2401 cggatgtccg ttgaagatcg aaggtggctg ctggaaaacg tgaaccgtcc gtgggaggtc
     2461 caccgctacc ggatggctcc gagcgtaggg gccgcgttcc accggctgat cgactcggga
     2521 agggtggaga tccagcaggc gagggtcggt gcgatcgaag aatccgggtc gcgggtcagg
     2581 gtcgaactgg tcggcccaaa gcgaagcgag tccagggaat acgaccgggt gatctcggcc
     2641 tgcggagcct gcacggacat ccgccgggat gccccggagc cggtcagggg gctgatcgat
     2701 tcgggccatc tgcgtcccga cgacctcctg ctcggcgtcg aagtagaccg cgacggggcg
     2761 gcccgggatg cccgtggcga tcgctcggcg acgatcagcg tgctgggctc gcttcgccgt
     2821 ggcgtcgact gggaatcgat cggggtgacc gaactcaaga cccagtcccg tgacatcgcc
     2881 ctgcgactgc tcgatcagat gtgatctgag gggccgggtg cttcgaggct gcggtagaca
     2941 ccctggtgaa ggggctgcgg atccgggccg cgccaaccgc ggcttctcag caactgaagc
     3001 gcctgcccga tcacttcggc ccggttgtcc cgaaattcct gacgcgtgaa ccggttcacc
     3061 tggtaaccca tcagcgtgtg tttgcggtcc ctcctggcat ctttggtctg catggccgga
     3121 tcccggtgga aggcatgccc atcgagctcg aacatcagca tgtactcctc ccagagaagg
     3181 tcgatcagac acccgtcgag caacgggtca acccggggtc gcggcgcacc agcacgaacc
     3241 agtgccccca cgaattcctt ctggaagatc agcatctcct cctccgtgga gggatgccag
     3301 tcacccaggg tcctgcgtag ccgagtgatt cccttcttgc ccgtccagcg gtccaactcc
     3361 cgttcgagtt ccttcaaacg gagcaagttc tcgcgatcgg catcagccag gtagtcgtca
     3421 agagcctcgc gggtcattcc cccggccagg tcgatcgtgg tccggatgaa gctcgtcacc
     3481 gggattccgt tcacctcggt tacttcacac gggtcgagtc gccgcgtgct gtgcacgatc
     3541 accttgccgt agccgtcaaa gggcttgagt tcccggttca ttccacctcg cttcaccaca
     3601 tgaatgacgg gccgcgcaga atcgagcttc caaagccagg ctgccgactg gtgagaaaga
     3661 caggcgtccg gtccagccgc cagaaccgcc gccatcgccc tcccccagac ggtcagctgg
     3721 cggggactgg tcgcgtacac cccatcgtgg atcgcagtca gccacccgga acgagccctg
     3781 gcgacgactg cgtcctttga cacgcccgcc ttagtcaatt ggaacatggc aacaacacca
     3841 tgttgagaac cgcaatagcg ggcaatgagg aagttggtct cacgagatgt ggccggctcg
     3901 gctaacgcca tcccccccag agtgccgtga attgcctcaa cagtgggtta cccaggtaac
     3961 gattgtgcaa ccgaaggctc acgaagtact gacctcctcc ctgtcctcga cttccgacca
     4021 attcagttcc acgactcaca accagacccg ccccaccagg tgcctatgag attcgttcat
     4081 atagacactt ccgataccca cctcgcgcgg gcgaggctcg tggcgttgag gtgtcgatgg
     4141 atttcgttca tatagacgaa atccatcgac acctctcggg caatgggaaa cgggcgcttc
     4201 gtgcaacgaa aaggggcgcc ggccggcgcc cctcttcgtg actcctggct gccggcttcc
     4261 cggcggccgt gattacttct cggtgctggc gtcgatctcc tgtgagatct cgtcgagctc
     4321 gtcggcgaag gattcgccga agccgtcggc tccgcccgct ccgttgccat cgccgttctc
     4381 gacgaggaga tcctcctcga gcaggaccgc ttcctccgcc ggagcgaagg gctcggtcgg
     4441 ctcgatggtc atcgagcggt agtgcttgag accggtcgca gccgggatca gcttgccgat
     4501 gatgacgttc tccttgaggc cgaccagacg gtcgcgcttg ccttccagcg cggcgtcggt
     4561 caggaccttg gtggtctcct ggaaggaggc cgccgagagg aacgacttgg tcgccagcga
     4621 ggccttggtg atgccgagga tcttctcctg ggccttcgcc gcggtgccgc cgtcggcctt
     4681 gacctgcttg ttgatcgcct tgagaacctg acggtcctcg agctggccgg gcaggagttc
     4741 ggtcgagccc ttggtttcga cttcgacctt cttcagcatc tggcgaacga tcacctcgat
     4801 gtgcttgtcg ttgatctcca cgccctgcgc cttgtacacg cgctggactt cggcgaccag
     4861 gtactgctcg accgcggtcc ggccacgaat cgagagcacg tcggccggat acagcgaacc
     4921 ttcgttcagc tgatcgccaa cctcgacctt gtcgccctgc ttgacgttga gtcgggtccg
     4981 gggcgggaat gtgtaggaac tctcctcgcc gttggactcg agcacggtga ccgtgatctg
     5041 cttctcggtc tgctcgacac cgaccttgcc ggccacttcg gccatctggg cgaggccctt
     5101 cggcttgcgc gcctcgaaca gctcgaccac tcggggcaga ccctgggtga tgtcctgtcc
     5161 ggccacacca ccggtgtgga aggtacgcat ggtcagctgg gtgcccggct cgccaatcga
     5221 ctgggcggcg atggtgccga tcgaatcacc gatctcgacc atgaagccgg tcgccggcga
     5281 gacgccgtag caggaacggc aaatgccgtg ctcggactcg cacttgaagg cggagcgcac
     5341 cggcaccgtg gccgattcat caccttcgta ggcgtcggtc agctcggccg catcggcctt
     5401 gctgatcagc tggttcttct tgagcagctc acgcccgcgc ttggtctcga acttcttcgc
     5461 cgaaaggcgg ccgaccacgt tcgggttcgg ctcgccgtcg atgaacagcg gcatgtcgat
     5521 cgattccttg gtcttgcagt cgtcctcgag gacgatcacg tcctgggcga cgtcaaccag
     5581 acgacgggtc aggtaacccg agtccgccgt acgcagagcc gtgtcggcca gacccttgcg
     5641 ggcaccgtgg gtcgagatga agtactcacc gaccgagagg ccctcgatga agttggactt
     5701 gatcggacgc tcgatgatct cgcccttcgg attcgccatc aggcctcgca tgccggccag
     5761 ctgacggatc tgcttgaacg aaccacgggc gccggagttg gccatcatga agatcgggtt
     5821 cagctgacgg aggttgtcct ccatcgcctg accgacttcc tcggtggccg cattccactt
     5881 gtccgtgacc tgcttgtggc gttcctcggc ggtgtgccag ccctcgtcga actcggcctg
     5941 gatccgggcg gtctcctcgt cgtacttggc gaggatctct tccttctccg gcggcggcac
     6001 gacgttgttc
//
LOCUS       DAOQKN010000153         5966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_6905_length_5966_cov_4.507867, whole genome shotgun sequence.
ACCESSION   DAOQKN010000153 DAOQKN010000000
VERSION     DAOQKN010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5966
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6905_length_5966_cov_4.507867"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1167
                     /locus_tag="PLJ64_09675"
     CDS             <1..1167
                     /locus_tag="PLJ64_09675"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HNC93792.1"
                     /translation="WILYSSGTTGLPKPIVQSQGGILLEHLKKMHLHLDAGEGDRIFW
                     FTTTGWMMWNFLVSCLLTDAAIVLYDGNPGHPDMSALWQLASDAGVTCFGTSASYISA
                     CMKDGVEPGADGRDLSKLRSVGSTGSPLAPEGFDWVYEHIGKDTWLFSTSGGSDLCTA
                     FVGGVPTLPVYRGELQARALGAAIESWDEEGNPHIGEVGELVITKPMPSMPLFLWGDE
                     DGSRYHDSYFDTYPGIWRHGDWIEITERGTAIIYGRSDSTINRGGIRMGTSEIYRAVL
                     ADEDIVDALVVDIPREGTEGWMPLFVVLAEGAELTDELKKKVAGEIRSRCSPRHVPNE
                     MIEIKAVPRTLSGKVLEVPVKRILTGTPAGKAASRDSLANPEALDFFSEYAKTL"
     gene            complement(1142..1792)
                     /locus_tag="PLJ64_09680"
     CDS             complement(1142..1792)
                     /locus_tag="PLJ64_09680"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNC93793.1"
                     /translation="MERSVWRYLFLAFLCASGTAALALLFYGSTRISEFDARVTSHFL
                     AVPDSRLDQVADFGARLAEPAPLILIAFGLIVLAVVWNRPWHLLAAGGVIIAANLTTQ
                     VMKALMAHPRLQGALGVSYPIEIHYPSGHTTAALASASGLWLVTPPRWRAWAAAFGAI
                     YALAVAAGVVIAGWHFISDVIGAALVVGFWACLAMAALVQAGLERTDRSRESSRTR"
     gene            complement(1814..3340)
                     /locus_tag="PLJ64_09685"
     CDS             complement(1814..3340)
                     /locus_tag="PLJ64_09685"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNC93794.1"
                     /translation="MTKVDLDPALRAALGGGPERHHAKTAEQGKLPVRERVDLLLDEN
                     SFVEDALLANWDQEGFGADGVVTGTGLIWGRPVALMANDPTVKAGSWGPKTVEKILRI
                     QERALKLEIPMIYLVDSAGARITDQVQMFPGRRGAGRIFYNEVKMSGRVPQVCILFGP
                     SAAGGAYIPAFCDVVIMRDGNASMYLGSPRMAEMVIGETVSLDEMGGARMHTSKSGCG
                     HFLVKTDEEAIETGRSYMRYMPTSWREQVPDVEPKKPVSDTPLEEIIPLDENVPFDIN
                     LLLDTLLDEGSFIEVHKRWAKELIVGYGRLDGKAVGIVANQPKQKGGVLFVDSADKAA
                     RFIATCNAFNIPLLFLADIPGFMIGTAVEKQGIIRHGAKMISAMSEATVPKISVVVRK
                     AYGAGLYAMAGPAFEPDSCIALPNASIAVMGPQAAVNAVFYNQIQAIEDEAEREKFVE
                     EKRKEYAEGVDLLHLASEMVIDAVIQPEDLRTELIKRFALAEGRDRSFTERRIPITPA
                     "
     gene            complement(3349..4233)
                     /locus_tag="PLJ64_09690"
     CDS             complement(3349..4233)
                     /locus_tag="PLJ64_09690"
                     /inference="COORDINATES: protein motif:HMM:NF005875.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HNC93795.1"
                     /translation="MEIKKVGVVGCGLMGHGITQVSAQAGYDVIVADVNQEALDKGLG
                     KISKLLGKSVEKGKIEQADADAILGRINTTTDLNDFADCDLVIEAVTENLETKLKIWK
                     QLDGIVKDEAYFATNTSSLPVINQAAATTRPTNFLGLHFFNPVQVMPLLEVIQSVTTS
                     PEAMGIGLAYGEKLKKTTVETKDKAGFIVNRLLVPYMLDAIRGLEEGIGTIDEIDAAM
                     KAGAGHPMGPLTLADFVGLDTLHSISDVMFDEFRERRFAAPPTLRKMVAAGFYGKKSG
                     RGFYDYSGEKPVSVNPGL"
     gene            complement(4312..4659)
                     /locus_tag="PLJ64_09695"
     CDS             complement(4312..4659)
                     /locus_tag="PLJ64_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93796.1"
                     /translation="MNRIRTFLIALVAIALVAGPIGLPSQAGASPDASASASAKAKKK
                     CKKFKGKKKRQCLKNASKGGGKPGNGGKPWAGSPYKPGITCAMSPEMQKKYARYGLTC
                     LDLGLGVTTLIEL"
     gene            complement(4656..5036)
                     /locus_tag="PLJ64_09700"
     CDS             complement(4656..5036)
                     /locus_tag="PLJ64_09700"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNC93797.1"
                     /translation="MKSDGFRAAAEAKDFEQGRDLFTEDVVFRSPFVHTPYEGIDALG
                     FLLGHVVQVFEDFHYIAHVETDDVAVLQFEARVGDKELQGVDILKFNDEGKICEMTVM
                     VRPASGLEAVGMAMKSRIEAATAS"
     gene            complement(5089..5817)
                     /locus_tag="PLJ64_09705"
     CDS             complement(5089..5817)
                     /locus_tag="PLJ64_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93798.1"
                     /translation="MKGFKRSPGVSILAAVVVLVLLGGGAATAAKWIDGKRIKPGSVA
                     TKQLKNKAVNTAKLRNAAVGTPQLKNGSVTAAKLAPSALTSGPTGPTGIAGSTGATGP
                     AGPSEVTWKRDTPNFLFSTGGSLVFSEGYPLGPAGYLIDGYLVIERDDVGDVNASCEI
                     FRVFVNSSDIQTGTAETVGLPRTTVAGNGFATLPISGVLDLSSPPGDADGVEMRVVCE
                     TNTDNAIAAERQVNAVPTDQLIPG"
BASE COUNT         1060 a         1910 c         1887 g         1109 t
ORIGIN      
        1 tggatcctct actcctccgg caccaccggg ctgccgaagc cgatcgtcca gagccagggc
       61 ggcatcctgc tcgagcacct gaagaagatg cacctccacc ttgatgccgg tgagggtgac
      121 cggatcttct ggttcacgac caccggctgg atgatgtgga actttctcgt ctcctgtctg
      181 ctcaccgacg cggcaatcgt gctttatgac ggcaaccccg gtcaccccga catgtcagct
      241 ctctggcagc tcgcttcgga tgccggggtg acctgcttcg gcacctccgc ctcctacatc
      301 tcggcctgca tgaaggacgg ggtcgagccc ggcgctgacg gccgtgacct ctcaaagctc
      361 cgctcggtcg gctcgaccgg ctcgccgctc gcccccgagg gcttcgactg ggtgtacgag
      421 cacatcggca aggacacctg gctgttttcg acctcgggtg gttccgacct ctgcacggcc
      481 ttcgtcggtg gcgttcccac ccttccggtc taccggggcg agctgcaggc ccgggcgctc
      541 ggggcggcga tcgagtcctg ggacgaagag ggcaacccgc atatcggcga ggtcggggaa
      601 ctggtcatca ccaagccgat gccgtcgatg ccgctgtttc tctggggcga cgaggacgga
      661 tcccgctacc acgacagcta cttcgacacc tacccgggaa tctggcggca cggcgactgg
      721 atcgagatca ccgagcgggg aaccgcgatc atctacggcc gctccgactc gaccatcaac
      781 cggggtggca tccgcatggg tacctcggag atctaccggg cggtactggc cgatgaggac
      841 atcgtcgatg ccctcgtggt cgacatcccc cgcgaaggaa ccgaaggctg gatgcccctc
      901 ttcgtcgtcc tggccgaggg ggccgaactg accgacgagt tgaagaagaa agtcgccggc
      961 gaaatccgca gccgctgctc cccgcgacac gttcccaacg agatgatcga gatcaaagcg
     1021 gtcccccgca ccctttcggg caaagttctc gaagttccgg tcaagcggat cctcaccggc
     1081 accccggccg ggaaggcggc cagccgcgac tcactggcca accccgaagc gctcgacttt
     1141 ttcagcgagt acgcgaagac tctctagagc gatcggttcg ttcaaggccg gcctggacca
     1201 gggcggccat ggcgaggcag gcccagaagc cgaccaccag tgccgccccg atcacgtcgc
     1261 tgatgaagtg ccagccggca atcaccacgc cggcggccac agcgagcgcg tagatcgccc
     1321 cgaaagccgc agcccaggcc cgccagcgtg gcggcgtcac cagccagagg ccggaagccg
     1381 aggcgagcgc ggcggtggtg tgaccgctcg ggtagtgaat ctcgatcggg tagctgaccc
     1441 cgagcgcccc ctggagccgg ggatgggcca tcagcgcctt catcacctgg gtcgtcaggt
     1501 tggcggcgat gatcaccccg ccggccgcga gcaggtgcca gggccggttc cagacgacgg
     1561 cgagaacgat caagccaaat gcgatcagga tcaggggagc cggctcggcc agcctcgccc
     1621 cgaagtcggc aacctggtcg agccgggagt ccggcacggc gaggaagtgg gaagtgaccc
     1681 gggcatcgaa ctcgctgatc ctggttgagc cataaaacag cagcgccaga gcggcggttc
     1741 cactggcgca gagaaacgcg aggaacagat atctccagac ggatcgctcc atccggaaac
     1801 cctacggaaa ccttcaggcg ggggtgatgg gaatgcggcg ctcggtgaag gagcggtcac
     1861 ggccctcggc cagggcgaag cgcttgatga gttccgtgcg gaggtcttcg ggctggatca
     1921 ccgcgtcgat aaccatctcg gaagcgaggt ggagcaggtc caccccttcg gcgtattcct
     1981 tgcgcttctc ctcgacgaac ttctctcgct cggcctcgtc ctcgattgcc tggatctggt
     2041 tgtagaagac cgcgttgacg gccgcctgcg gccccatcac ggcgatcgag gcgttcggca
     2101 gggcgatgca ggaatcgggc tcgaaggccg ggccggccat cgcgtagaga ccggctccgt
     2161 aggccttgcg aacgacgacc gagatcttcg gcactgtcgc ctcggacatc gccgagatca
     2221 tcttggcacc gtgacggatg atgccctgct tctcgaccgc ggtgccgatc atgaaaccgg
     2281 gaatgtcggc caggaagagc agggggatgt tgaaggcgtt gcaggtagcg atgaagcgag
     2341 ccgccttgtc ggccgagtcg acgaagagca cgccgccctt ctgcttcggc tggttggcga
     2401 ctatcccgac cgccttgccg tcgagccggc cgtagccgac gatcagctcc ttggcccagc
     2461 gcttgtgaac ctcgatgaag ctgccctcgt cgaggagcgt gtcgagcagg aggttgatgt
     2521 caaaggggac gttctcgtca agcggaatga tctcttcgag cggggtgtcg gagaccggct
     2581 tcttcggctc gacgtcaggg acctgctcgc gccagctggt cggcatgtac cgcatgtagc
     2641 tgcgaccggt ctcgatcgcc tcttcatcgg tcttgaccag gaagtggccg caacccgact
     2701 tggaggtgtg catgcgggcc ccgcccatct cgtcgagtga aaccgtctcg ccgatgacca
     2761 tctcggccat gcggggagaa ccgaggtaca tcgaggcgtt tccgtcccgc atgatcacga
     2821 catcgcagaa ggcgggaatg taggcaccgc cggccgcgct gggaccgaac aggatgcaga
     2881 cctgcggtac ccggccgctc atcttcactt cgttgtagaa gatccggccc gctccgcggc
     2941 ggccggggaa catctggacc tgatcggtga tccgggcccc ggccgagtcg accaggtaga
     3001 tcatcgggat ctccagcttc agggcccgtt cctggatccg gaggatcttc tcgaccgtct
     3061 tcgggcccca ggaaccggcc ttgacggtcg gatcgttggc catcagggca accggccggc
     3121 cccagatcag cccggttccg gtgacgacgc catcggcgcc gaagccctcc tggtcccagt
     3181 tggccaggag cgcgtcctcg acgaaagagt tttcgtcaag cagcagatcg acccgttccc
     3241 ggaccgggag cttgccctgc tccgcggtct tggcgtgatg cctttccgga ccgccgccga
     3301 gggcggcccg gagggcggga tcgagatcga ccttggtcaa cggaagccct acaggcccgg
     3361 attgaccgag accggctttt cgccggagta atcgtagaaa ccgcggcccg acttcttgcc
     3421 gtagaaaccg gcggcgacca tcttgcgaag ggtcggcggg gcggcgaaac gacgctcacg
     3481 gaactcgtcg aacatcacgt cggaaatcga gtgaagggtg tcgagcccga cgaagtcggc
     3541 cagggtgagc gggcccatcg ggtgaccggc accggccttc atcgccgcat cgatctcgtc
     3601 gatcgtgccg atgccctctt cgaggccgcg gatcgcgtcg agcatgtacg gaaccagcag
     3661 gcggttgacg atgaagccgg ccttgtcctt ggtttcgacg gtggtcttct tcagcttttc
     3721 gccgtatgcg agaccgatgc ccatcgcctc gggcgaggtg gtcaccgact ggatcacctc
     3781 gagcaggggc atgacctgga ccgggttgaa gaagtgcagg ccgaggaaat tggtcggccg
     3841 tgtggtggcc gcggcctggt tgatgaccgg cagcgaggaa gtgttggtcg cgaagtaggc
     3901 ctcgtccttg acgatgccgt cgagctgctt ccagatcttg agcttggtct cgagattctc
     3961 ggtcacggcc tcgatcacaa ggtcgcaatc ggcgaagtcg ttcagatcgg tggtggtgtt
     4021 gatccggccg aggatcgcgt cggcgtcagc ctgttcgatc ttgcccttct cgaccgactt
     4081 gccgagcagc ttgctgatct tgccgaggcc cttgtcgagc gcttcctggt tgacatcggc
     4141 aacgatgacg tcgtatccgg cctgggcgct gacctgggtg atgccgtggc ccatcagacc
     4201 gcagccaaca accccaacct tcttgatctc catctggagc cttcctcctg tagcgaagtt
     4261 cgtgaatgcc cccgggacgg cggaggcgac cggccacgga gcctattcga tctaaagctc
     4321 gatcagggtg gtgacgccga ggcccagatc gagacaggtc aggccgtacc tggcgtactt
     4381 cttctgcatc tcgggactca tcgcgcaggt gattccgggc ttgtagggac tgccggccca
     4441 cggctttccg ccattgcccg gctttccgcc acccttcgaa gcgttcttca ggcactgtcg
     4501 cttcttcttg cccttgaact tcttgcactt cttcttcgcc ttggccgaag ccgaagctga
     4561 ggcgtccggt gaagcaccag cctgtgatgg caggccgatc ggaccggcga ccagggcgat
     4621 cgccaccagg gctatcagga aggtccggat gcggttcacg aggccgtcgc cgcttcgatc
     4681 cggctcttca tagccatgcc gacggcctcg agaccggaag caggcctgac catgacggtc
     4741 atttcgcaga tcttgccttc gtcgttgaac ttgaggatgt cgacgccctg aagttccttg
     4801 tctccgaccc gggcctcgaa ctggagcacg gcaacatcgt cggtctcgac gtgggcgatg
     4861 tagtgaaaat cctcgaagac ctggaccaca tggccaagca ggaaaccgag cgcgtcgatg
     4921 ccctcatacg gggtatgaac gaaggggctg cggaagacaa catcctccgt gaacagatcc
     4981 ctgccctgct cgaaatcctt tgcttccgcc gctgccctga aaccgtcgct cttcatatgc
     5041 cgaaacatat agctgactga aacacgcgtg ctttctgtgc cgcgttagct accccgggat
     5101 cagctggtcg gtgggtaccg cgttcacttg gcgctctgcc gcaatagcgt tatctgtgtt
     5161 tgtctcgcag acgactctca tttcgacacc gtcggcgtca ccgggggggc ttgaaaggtc
     5221 aagtactccg ctgatgggca gggttgcgaa tccgttaccg gcaaccgttg ttctgggcag
     5281 ccccaccgtc tccgccgtcc cggtctggat gtcggacgaa ttcacgaaga cccggaaaat
     5341 ctcgcagctg gcgttcacgt ctccaacatc gtcgcgttcg atcaccaggt atccgtcaat
     5401 caggtaacca gctggcccca gtggatagcc ctcactgaag accagagagc ctcctgtcga
     5461 gaacaggaag ttgggtgtgt cccgcttcca ggtcacttcc gaaggcccgg cgggtccggt
     5521 ggcaccagtg cttcccgcga tgccagtcgg gccggttggc ccagacgtca gggccgaagg
     5581 tgccagcttt gccgcagtca ccgaaccgtt cttgagctgt ggtgtgccaa cagccgcgtt
     5641 tctcagcttg gcggtgttca cagctttgtt cttcagttgc ttggttgcga ccgagccagg
     5701 cttgattcgc ttgccatcaa tccattttgc tgccgtcgcc gcgccgcccc caagcaggac
     5761 caacacgaca accgcggcca agatcgatac cccgggactg cgcttgaaac ccttcatcac
     5821 tctccctggt ggccggctgt tcccggccag atagacgatc cgcaaattct aagtgccgct
     5881 ctaccgtctc gtcaaggatt ccgctcggaa aggtccttcc agtcaatggg cccggccttg
     5941 aggacgtggg aaccgagagg tcggcc
//
LOCUS       DAOQKN010000154         5874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_7081_length_5874_cov_3.446812, whole genome shotgun sequence.
ACCESSION   DAOQKN010000154 DAOQKN010000000
VERSION     DAOQKN010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5874
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7081_length_5874_cov_3.446812"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="PLJ64_09710"
     CDS             complement(<1..690)
                     /locus_tag="PLJ64_09710"
                     /inference="COORDINATES: protein motif:HMM:NF018332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC93799.1"
                     /translation="MTRSTYLAMVAMALGVFIIANDITAMNVALPAIEKDFDTSVTTV
                     QWVVNAYALVFGVLIVTGGRLADLFGRREAFFVGAGIFLVASLAAATAQTEAWLIAAR
                     AVMGVGGALIWPAVLGMTFALLPEEKAGLAGGLILGVAGIGNALGPLIGGALTEFESW
                     RWILFLNVPICLIAIAAVYFLIHQPRPETEDRKIDYGGIATVSFGLVLFMVALDQVVD
                     LGWGDPRIVISV"
     gene            807..1535
                     /locus_tag="PLJ64_09715"
     CDS             807..1535
                     /locus_tag="PLJ64_09715"
                     /inference="COORDINATES: protein motif:HMM:NF025049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNC93800.1"
                     /translation="MPVRQEQRQEQEEQEEQGKQQGFGRSGVSRRQLMSMAGGSVALG
                     AFLAACGDDSDSDSNPAMSGSSGQSGSTGSSMSDDSTAQFGEGDIGIMNYALTLEYLE
                     TAFYDDVAKSGLFKGADLETIRKFGSQEAEHVAALTAAVKKAGGKPAPKPKTEFPLNN
                     AQQVLRLASTVENLGAAAYLGQAARIQSPEVLAAALSIHSVEGRHAAALNTLLGKTPV
                     PDGPFAQPAPAAMVLNAVQPFIVS"
     gene            1562..2206
                     /locus_tag="PLJ64_09720"
     CDS             1562..2206
                     /locus_tag="PLJ64_09720"
                     /inference="COORDINATES: protein motif:HMM:NF025049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNC93801.1"
                     /translation="MTEIKTDTEMVHPELAAIEVDGTERSDILMKGALAAGAIFGLGA
                     VSPFVKGAMGAGGSKGDVEILNYALTLEYLESAFYAEALKKAGLSGELKELVKTISTD
                     EDAHVKALTQTIRDLGGKPVKAPKVEFPYSDTAGFTALAETIENVGVSAYNGAAPMIS
                     SKEVLEAAGSIVQVEARHAAAIALQNGNQPAPDAFDKTMTQAQVLKAVQPFLVS"
     gene            2243..2833
                     /locus_tag="PLJ64_09725"
     CDS             2243..2833
                     /locus_tag="PLJ64_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNC93802.1"
                     /translation="MNNRVPAQPTDPDEDLLVRVGGRDKKAFELFYDRFERRAFSLAY
                     RIVGERSLAEDIVQEGFLSIWKNAGRYDPARGSAGAWALGIVRNRSIDVLRSRASRTP
                     DLDHDDDSTLENRASALKTEEQALQREQGREVRSRLSTLPENQSKVIGLAFYGGFSQT
                     EISDMLELPLGTVKGRMRLGMEKLRVELGSYEGEAK"
     gene            2830..3570
                     /locus_tag="PLJ64_09730"
     CDS             2830..3570
                     /locus_tag="PLJ64_09730"
                     /inference="COORDINATES: protein motif:HMM:NF021604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HNC93803.1"
                     /translation="MTSGHENFKGQLPAYLLGALEPGERADLEHHLEGCAECKAELAW
                     LEPATSTLAADVEQFAPSPELKQRVMAAVDADLEQHPLPVPDPIEVQAPSRKERERRS
                     RGWLSGVFRPAVIGVVAAALFVGVVAGVALNGGGESSPEGPTRQVVTGSSTIGADAVM
                     VASDGTGTLKMSNLKPPDEDQVYQAWIQRGQEIVPTESLFVPNRKGSAVASIPDMSGV
                     TAVMISAEPKGGSKQPTTAPVITVSMES"
     gene            3649..4125
                     /gene="greA"
                     /locus_tag="PLJ64_09735"
     CDS             3649..4125
                     /gene="greA"
                     /locus_tag="PLJ64_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNC93804.1"
                     /translation="MTDSEETGESITAEGLAQLKAELEELEGPAREKIAARLKSAREL
                     GDLKENAEYHIAKEDQAHLETKIQRLREREKNAVVAEVDSSGDSFAFGRTAEVTDDSG
                     RSHRWTLVGSTEANLAEGRLSAESPVGQALRNRKVGEEVKIATPKGERSFTINSVS"
     gene            4169..4450
                     /locus_tag="PLJ64_09740"
     CDS             4169..4450
                     /locus_tag="PLJ64_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93805.1"
                     /translation="MAKKKSKANQAGAAVGLAAKAWEYGRKVDWPAVWMRARWLTHHT
                     QRLYNNLTPAERKEFLDIVVPTKSSKLVAKEDRARVTELVTKAFTGNQK"
     gene            complement(4526..4984)
                     /locus_tag="PLJ64_09745"
     CDS             complement(4526..4984)
                     /locus_tag="PLJ64_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93806.1"
                     /translation="MKLKAKIGAFVATAAVACAIGAAPAQAAPSAPALPSAGDLSALC
                     NKVSNAKLKSACNQGVAAYNSCSGQTSSKGLLSCLGNAAKGIIGSGVPTGGSSSDIIA
                     KLKSLIGGAGTGGLNLGGLSLGGFDLSSILSKLGGAGGLNLGSLLGKLGG"
     gene            5218..>5874
                     /locus_tag="PLJ64_09750"
     CDS             5218..>5874
                     /locus_tag="PLJ64_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93807.1"
                     /translation="MFRTILIVLVAGSVCAAAAPADAVAYGKPGPGTALAGCPVFPAG
                     NAFNRDISGAKVHPLSGRYIASIGRGSMLHPDFGSGRYGNYGIPFRVVSRRQRRIPVL
                     FTAFGSESDRGPYPIPLDTRIEGGSDDHVLAIQRGTCRLFELYRARRGKGRWLADSGA
                     KFNLRSNRLRPAGWTSADAAGLPILPGLARAGEASSGRITHALRVTVSETQRGYILPA
                     R"
BASE COUNT         1141 a         1907 c         1939 g          887 t
ORIGIN      
        1 gaccgagatc acgatccgcg ggtcacccca gccgaggtcc acgacctggt cgagggcaac
       61 catgaagagg accagcccga acgagacggt ggcgatcccg ccgtagtcga tcttccggtc
      121 ctctgtttcc ggccggggct ggtgaatcag gaagtagacc gcggcgatcg cgatcaggca
      181 gatcggcacg ttgaggaaga ggatccagcg ccagctttcg aactcggtca gggcgccgcc
      241 gatcagcggg ccgagtgcgt tgccgattcc ggccaccccg aggatgagcc cgccggccag
      301 cccggccttc tcctctggca ggagtgcgaa ggtcatgccg aggacggccg gccagatcag
      361 ggcgccgccg actcccatga ccgcccgggc ggcgatcagc caggcttcgg tctgcgcggt
      421 ggctgcggcg agggaggcga ccaggaagat tccggccccg acgaagaacg cctccctccg
      481 gccgaacagg tcggcgagcc tgccgccggt gacgatcagc accccgaaga cgagggcgta
      541 cgcgttgacg acccactgga cggtggtgac gctggtgtcg aagtcctttt cgatcgccgg
      601 caaagcgacg ttcatcgccg tgatgtcgtt ggcgatgatg aacaccccca gcgccatcgc
      661 gaccatggcg agataggtcg aacgagtcac accacaacct tacgggctgg cggcgacgct
      721 gaaaagaggc tcgcaactgc cactgatccg atcccctgcc tcccccgtaa aaccggtcaa
      781 cggcgtaaac aaacagagga gacgccatgc cggtaaggca ggaacaaaga caagagcagg
      841 aagaacagga agagcagggc aagcagcagg gattcggcag aagcggggtg tctcgccgcc
      901 agttgatgtc catggccggc ggttcggtcg ccctcggagc gttcctggcg gcctgcggcg
      961 acgactcgga ttccgattcg aacccggcga tgtccggaag ctccggccaa agcggctcga
     1021 ccggttcgtc gatgagcgat gacagcaccg cgcagttcgg cgagggcgac atcggaatca
     1081 tgaactacgc cctgaccctc gagtacctgg agacggcctt ctatgacgat gtcgcgaaaa
     1141 gcggactctt caagggcgcc gacctcgaga cgatcaggaa attcggtagc caggaagccg
     1201 agcacgtggc cgcgttgacc gccgccgtga agaaggcggg cggcaagccg gcaccgaagc
     1261 cgaagaccga gttcccgctg aacaacgcac agcaggttct gcgactggct tccacggtcg
     1321 agaatctcgg cgccgcggcc tacctcggcc aggccgcccg gatccagagc ccggaagtgc
     1381 tggccgcagc cctctcgatc cacagcgtcg aaggccgcca cgcggcggct ctcaacacgc
     1441 tgctcggaaa gacgccggta cccgacgggc ccttcgccca gccggccccg gcggcgatgg
     1501 tcctgaatgc cgtccagccc ttcatcgtca gctgaacgct ggcaggaaag gaaacgatca
     1561 gatgactgaa atcaagacag ataccgaaat ggtccatccc gaactcgccg caatcgaggt
     1621 cgatggaacc gaacgcagcg acatcctgat gaaaggcgcc ctggccgccg gggcgatctt
     1681 cggactcgga gcggtcagtc ccttcgtgaa gggtgcgatg ggagccggcg gcagcaaggg
     1741 tgacgtcgag atcctcaact acgcgctgac cctggagtac ctcgagtccg cgttttacgc
     1801 ggaggccctg aagaaggccg ggctctccgg ggagctgaag gagctggtga aaaccatctc
     1861 gaccgacgaa gatgcccatg tgaaggcgct cacccagacg atcagggatc tcggaggcaa
     1921 gccggtcaag gcgcccaagg tcgagttccc gtactcggac accgccggct tcacggcgct
     1981 ggccgaaacg atcgagaacg tcggcgtcag cgcctataac ggcgccgccc cgatgatcag
     2041 ctcgaaggaa gtgctcgagg cagcggggag catcgtccag gtcgaggccc ggcacgccgc
     2101 cgcgattgcc cttcagaacg gcaatcagcc ggccccggac gctttcgaca agaccatgac
     2161 ccaggctcag gtgctgaagg ccgtgcagcc gttcctggtc agctgagatt cccgggtagg
     2221 gaatggggaa cctgacatgg agatgaataa cagggttccc gcgcagccga ccgacccgga
     2281 cgaggacctc ctcgtccggg tcgggggccg cgacaagaaa gccttcgagc ttttctacga
     2341 ccggttcgag cgacgggcct tttcactggc ctaccggatc gtcggcgagc ggtcactggc
     2401 cgaggacatc gtccaggaag ggttcctctc gatctggaag aacgcgggcc ggtacgaccc
     2461 ggcccggggc agcgccggag cgtgggcgct ggggatcgtg cgaaaccgtt cgatcgacgt
     2521 gctccggagc cgtgccagtc gcaccccgga cctcgaccac gatgacgatt ccaccctcga
     2581 gaacagggct tcggctttga agaccgagga acaggcgctt cagcgcgagc agggccgcga
     2641 ggtgcgctca agactttcga cgctcccgga aaaccagtcg aaggtgatcg gactcgcctt
     2701 ttacgggggt ttcagccaga ccgagatatc cgacatgctc gagttgccgc tcggcacggt
     2761 caagggaagg atgcggctgg gcatggagaa gctgcgggtt gagttgggct cttacgaagg
     2821 agaggcgaaa tgaccagcgg gcacgagaat ttcaaagggc aattgccggc ctacctgttg
     2881 ggggcgcttg aacccggtga acgggccgac ctcgagcatc acctcgaagg ttgtgccgaa
     2941 tgcaaggcgg aactcgcctg gcttgagccg gctacctcca ccctggcggc cgacgtcgaa
     3001 cagtttgcgc cttcccccga gctgaagcag agggtgatgg cggcggtcga cgccgacctc
     3061 gagcagcacc cgctgccggt gccggacccg atcgaggtcc aggcgccctc ccggaaggaa
     3121 agggagcgcc ggagccgcgg ctggttgagc ggggtcttcc ggccggcggt gatcggcgtc
     3181 gtggctgcgg cactcttcgt cggcgtcgtg gccggagtcg cgctgaacgg tggcggggaa
     3241 tcgtcgccgg aaggcccgac ccgccaggtc gtgaccggtt cctcgacgat cggagccgat
     3301 gccgtcatgg tcgcttcgga tggaaccggc accctgaaga tgtccaatct caagccaccc
     3361 gacgaagacc aggtctacca ggcgtggatc cagcggggtc aggaaatcgt gccgaccgaa
     3421 tcactcttcg taccgaaccg gaagggttcg gcggtggcga gcatccctga catgagcggg
     3481 gtcaccgccg tgatgatcag cgccgaaccg aagggcggca gcaaacagcc gaccaccgct
     3541 ccggtgatca cggtctcaat ggagagctga tttccatcca gtgcgaacgg cccgggcgat
     3601 cctctctcca gagcccgggc cgcctcgccc cccgggtagg ctgcgtccat gactgattca
     3661 gaggaaaccg gcgagtcaat caccgccgaa gggctggcac agctgaaggc cgaactcgag
     3721 gagctcgagg gtccggcccg ggagaagatc gccgcccggc tgaagagcgc ccgtgaactc
     3781 ggcgacctga aggagaacgc cgagtaccac atcgccaagg aggatcaggc ccacctggaa
     3841 acgaagatcc agcggttgcg cgaacgtgag aagaacgcgg tcgtggccga ggttgactcc
     3901 tccggtgact cctttgcctt tggccggacc gccgaagtga ccgacgattc cggccgcagc
     3961 caccggtgga ccctggtcgg ctcgaccgag gcaaacctgg ccgagggcag gctgagcgcc
     4021 gaatcaccgg tcggccaggc actccgcaat cggaaggtcg gcgaagaggt gaagatcgcc
     4081 acccccaagg gcgagcgcag tttcacgatc aactccgttt cctgaccgga gatcaccggc
     4141 cggaccaacc gggccttatc ctccagccat ggcaaagaag aaatcgaagg caaatcaggc
     4201 gggggccgcc gtcggcctcg ctgcgaaggc ctgggagtac ggccggaagg ttgactggcc
     4261 ggccgtctgg atgcgggccc gctggctcac ccaccacacg cagcggctct acaacaacct
     4321 gaccccggcc gaacgcaagg aatttctcga catcgtcgtg ccgaccaaga gttcaaagct
     4381 cgtcgccaaa gaagaccggg cccgggtcac cgaacttgtg accaaggcct tcaccggcaa
     4441 ccagaagtag ccggcccggg ccgaaccctt aaagcagaac gccggccgcg gacagtcgcg
     4501 gccggcgctt tgcgttgggg ctttctcagc cgccgagctt gcccaggagg ctgcccagat
     4561 tgaggcctcc cgcaccgccc agcttgctga ggatggacga gaggtcaaag ccaccgaggc
     4621 tcaggccacc gaggttgagg ccaccggtgc cggcgccgcc gatcagcgac ttgagcttgg
     4681 cgatgatgtc cgagctgctg ccaccggtcg gaacacccga accgatgatg cccttggcag
     4741 cgttgccgag gcaggaaagc agacccttcg agctggtctg gccgctgcag ctgttgtagg
     4801 ccgcgacacc ctggttgcag gctgacttca gcttggcgtt gctgaccttg ttgcagagcg
     4861 cgctcaggtc acccgcgctc ggcagggccg gggccgacgg agcggcctgg gccggggctg
     4921 caccgatcgc acaggcgacg gcagcagttg cgacgaaagc accaatcttg gctttaagct
     4981 tcatctagtt atctcctcct gggtaaatcg ttctccctac ccaccagaaa atgtagcgca
     5041 aaaaccggcg ctcatgaaca tttgtttcat gaaacttgcc gtcctcatga acaagtaggt
     5101 acggaacgtc cgtgtccggc tccagcactg gaaatcagga cctttcccgg caagctcccg
     5161 atttaccccc gaaggcgtgt tccgggccgg atttcccgtc ttcgtagcat tggggcaatg
     5221 ttccgaacga ttctgatcgt cctggtcgcc ggttcggtgt gcgccgcggc cgcccccgcc
     5281 gacgccgtcg cgtacggcaa acctggcccc gggacggccc tggccggctg ccccgtcttc
     5341 cccgccggca acgccttcaa ccgcgacatc tcgggggcga aggtccaccc gctttccggc
     5401 cgctacatcg cctcgatcgg gcgcggttcg atgctccatc cggacttcgg gtcgggccgg
     5461 tacggcaatt acgggatccc gttccgggtg gtcagtcgca ggcagcgccg gatcccggtc
     5521 ctgttcaccg ccttcggatc ggagagtgac cgcgggccgt atccgatccc cctcgacacg
     5581 agaatcgaag gcggttcgga tgaccatgtg ctcgcgatcc agcgagggac ctgccggctg
     5641 ttcgagctct atcgcgcccg ccggggaaag ggccgctggc tggctgattc gggggcgaag
     5701 ttcaacctgc gaagcaaccg cctgcgcccg gccggctgga ccagcgccga cgcggccggg
     5761 ctgccgatcc tgcccgggct tgcccgggcc ggcgaggctt cgagcggcag gatcactcac
     5821 gccctccggg tcaccgtctc ggagacccag cggggctaca tcctccccgc ccgc
//
LOCUS       DAOQKN010000155         5851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_7119_length_5851_cov_5.184783, whole genome shotgun sequence.
ACCESSION   DAOQKN010000155 DAOQKN010000000
VERSION     DAOQKN010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5851
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7119_length_5851_cov_5.184783"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..648)
                     /locus_tag="PLJ64_09755"
     CDS             complement(76..648)
                     /locus_tag="PLJ64_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HNC93808.1"
                     /translation="MDEFFFDLGSPYAWLSAERIDSLFESAGRELPVWRPVLLGGLFQ
                     RFGRGSWAKTDGREAGISEIERQAREYGLPEPVWPDPWPGSYLFAMRVATFAEREGRL
                     KEFALAAFRDAFLRGVDLAIPEHVLNAAAAVGLDPVVAGAAAATVEVKDALKQATRRA
                     GDLGVTGVPSFLVNGEVYWGDDRLDEAVSG"
     gene            complement(756..2126)
                     /gene="lpdA"
                     /locus_tag="PLJ64_09760"
     CDS             complement(756..2126)
                     /gene="lpdA"
                     /locus_tag="PLJ64_09760"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNC93809.1"
                     /translation="MADESFDVIVIGAGPGGYVAAIRAAQLGKTTAVVESDKAGGRCL
                     NYACIPAKTMLHTAEVLDEARNSADIGVKVQGAELDWDALGQRRTDVSASLSGGVKGL
                     FDKNKIRFIEGEGSLTADGDVEVGGTVYGAGKVILATGSVAQAIPGVDFSDRVLDTWG
                     AWSLAEQPKKIAVVGAGASGSEIASAYIRYGTEVILVEMLDQILPVEDKDVVRVVERT
                     FKKQGIEISTGTPVENVEAGKDSVRFTYGENSAEVDYLVIAGGRRPDVDALGLDAAGV
                     KLDENGRVAVDGFQKTSNDKVYAIGDLVRGSALAHKAMEEGVVAAESAAGAETHPVDL
                     DLVPGATFCHPQVASVGLTEAQAKEQGLDVKTGKLKLGGVGAGTVYDDRDGMVKLVVD
                     KEYGEIVGAHIVGNRACDMIAELVAVMALEGGIQELQRIMHPHPTISEAVLDAARAVD
                     KWATHA"
     gene            complement(2146..3147)
                     /locus_tag="PLJ64_09765"
     CDS             complement(2146..3147)
                     /locus_tag="PLJ64_09765"
                     /inference="COORDINATES: protein motif:HMM:NF015709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family acid phosphatase"
                     /protein_id="HNC93810.1"
                     /translation="MKNSLAGFRSIIAALALFLALGFALAPAASADTYTPQQRKEYFD
                     SGKYANDLAFVAQAGRRWIVKRTQPTIGKVRACRKAHFPIGKADPGKDPAADYTVPVA
                     VPANYQPTAPIVAAPDPGPDGQAVTLLPDNSTLEPTKPVRKITRKACAGMKKLAIAMD
                     MDETAMSSFRYGSPQPNYDTISMYRNEILGSQTALKPMLNLFKLARKRGMATFVITAR
                     YEPISADPLVGALMGSADLCSSSLAWTGACGLDINTFNFTKVTWANLKDEGYTGLTDL
                     YMRPPDSESKGEVKNSERAEISLRRGYRIIAMFGDQNSDLEKGFYERGFKYQSPEGD"
     gene            complement(3225..3809)
                     /locus_tag="PLJ64_09770"
     CDS             complement(3225..3809)
                     /locus_tag="PLJ64_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93811.1"
                     /translation="MAEQEPRRPARRKARGSKRVADEAAGVPSANGNGRPAGQSGAEA
                     MKAGYAEAEVKNQAAREALQPLVEGERPTVVTVGAIFSAVVAVLLWGSTLYALFTGAE
                     AGGREVNVFQWAFFALVISAMAWGMWKAKYWAVLGFQMLLVLLILAGVLGMVTAPTLM
                     QIIGTLILTAGLSVLFWFMVKAMARIQMPDRPGS"
     gene            complement(3802..4506)
                     /gene="thiE"
                     /locus_tag="PLJ64_09775"
     CDS             complement(3802..4506)
                     /gene="thiE"
                     /locus_tag="PLJ64_09775"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HNC93812.1"
                     /translation="MSFEPADGAGGLRRERLRTARLYFVCDAAPNGNDPEPLLKAALD
                     GGVDIVQLREKEADTRVIERAADTFRRVCDTYGVPFILNDDPELAMSVRADGVHIGQD
                     DISPAEAREIIGPNHLLGLSTHSEEQIAAAHQTVRDGTPIDHISVGPINETPTKPGRP
                     ATGLELIRHAASTTTIPFFAIGGIDPDNVPQVLQAGARRICVIRAIHDAADPTAVAAS
                     LRRSFASVGMEVLPGG"
     regulatory      4547..4660
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4669..5817
                     /gene="thiO"
                     /locus_tag="PLJ64_09780"
     CDS             4669..5817
                     /gene="thiO"
                     /locus_tag="PLJ64_09780"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HNC93813.1"
                     /translation="MNRAEPREESDVIVVGAGLIGLACAWELAGSGVSVTVFERDEPG
                     CGATRVAAGMLAPIGELDFGEPDLLALNLRSRRLYREFTGEVERATGLDTGYRHTGAL
                     HVALDRDEAGELKREMELQRSHGLESKWLGPIAARDLEPGVSPSLAGAVLVEEDAVVD
                     PRALVEALRAGLAQRGVEIVSAEVTGLERDSAGRVRGVGTSIGSFAAATVVAAPGAVA
                     GVAGWLPEEVRPPVRPVKGQTVELRGPSEDPVCERILGSERVYIAPRPDGRVILGATS
                     EEMGFDTRVTGGGVHELLREAYRLLPDVAEMEFVGAVAGLRPGTPDNLPIIGRTGIEG
                     LILATGHYRNGILLAPVTAEAIAALVAGDQPEWLEAASPGRFTKQEVG"
BASE COUNT          938 a         1931 c         2010 g          972 t
ORIGIN      
        1 atgggtgacc tgagcggaaa ggtgtaccgg gtcaaggcct agccgacaca gcagcccggc
       61 cggagccgcc ggagctcagc cgctgactgc ctcgtccagg cggtcgtcgc cccaatagac
      121 ctcgccgttg acgaggaagg aagggacgcc tgtcacaccg aggtcgccgg ctcgccgggt
      181 ggcctgcttg agggcatcct tcacttcgac cgtggcggcg gcagctccgg cgaccaccgg
      241 gtcgagtcca acggctgcgg cagcgttcag cacatgctcg ggtatcgcca ggtccacccc
      301 gcggaggaag gcgtcgcgaa aggccgcaag cgcgaactcc ttcaggcggc cttcccgctc
      361 ggcaaaggtg gcgacccgca tcgcaaacag gtaactgccc ggccacggat ccggccagac
      421 cggttccggc aacccgtact cccgggcctg ccgctcgatt tccgagatcc cggcttcgcg
      481 accgtcggtt ttcgcccacg acccgcgacc aaaccgctgg aacagcccac cgagaagcac
      541 cggccgccag accggaagct cccgccccgc cgactcgaac agcgagtcaa tccgctccgc
      601 actcaaccac gcataaggcg acccgagatc aaaaaagaac tcgtccatca agggcccgaa
      661 cctttctcaa accggacccc acccaaccat cagatgattc gagtgggcgg ggcggtgcca
      721 tatggagggc gggtcggacc ccgcccgact cctgctcaag cgtgggtggc ccacttgtct
      781 acggccctgg cggcgtcgag gacggcttcg gagatcgttg ggtgggggtg catgatgcgc
      841 tggagctcct ggatgccgcc ttcgagggcc atcacggcga ccagctcggc gatcatgtcg
      901 caggcccggt tgccgacgat gtgggcgccg acgatctcgc cgtactcctt gtccacgacc
      961 agcttgacca tgccgtcgcg gtcgtcgtag acggtgccgg ccccgactcc gccgagcttg
     1021 agcttgccgg tcttcacatc gaggccctgc tccttcgcct gggcctcggt cagcccgacg
     1081 ctggcgacct gcgggtggca gaaggtggcg cccgggacca ggtcgagatc gaccggatgg
     1141 gtttcggcgc cggcggcgga ctcggccgcg accacgccct cctccatcgc cttgtgggcg
     1201 agggccgacc cgcggaccag gtcaccgatc gcgtagacct tgtcgttgga agtcttctgg
     1261 aacccgtcga cggcgacccg tccgttctcg tccagcttca ccccggccgc gtcaagacca
     1321 agcgcgtcca cgtccggccg gcggcccccg gcgatcacca ggtaatcgac ctcggccgag
     1381 ttctcgccgt aggtgaacct gaccgagtcc ttgccggcct caacgttctc gaccggcgta
     1441 ccggtggaga tctcgatgcc ctgcttcttg aaggtgcgtt cgaccacccg gaccacatcc
     1501 ttgtcctcga ccggcaggat ctgatcgagc atctcgacca ggatcacttc ggtcccgtag
     1561 cggatgtagg cggaagcgat ctcggagccg gaggcaccgg cgccgaccac ggcgatcttc
     1621 ttcggctgtt cggccagaga ccaggcaccc caggtgtcca gcacccggtc ggagaaatcg
     1681 accccgggaa tcgcctgcgc taccgaaccg gtggcgagga tcaccttgcc ggccccgtac
     1741 acggtgccac cgacctcgac gtcaccgtcg gcggtcaggc tgccttcgcc ttcgatgaac
     1801 ctgatcttgt tcttgtcgaa gagtcccttg acgccgccgg acaggctggc cgagacatcg
     1861 gtccggcgct ggccgagcgc gtcccagtcg agttcggctc cctgcacctt gaccccgatg
     1921 tccgccgagt tcctggcttc atcgagaacc tcggcggtgt ggagcatcgt cttggccggg
     1981 atgcacgcgt agttcagaca gcgtccgccg gccttgtccg attcgaccac cgcggtggtc
     2041 ttgccgagct gggcggcgcg gatcgcggcc acatagccgc ccggtcccgc accgataacg
     2101 attacgtcaa acgattcatc agccatggtc aggcgagact atcttctagt cgccttcggg
     2161 gctctggtac ttgaagccgc gctcgtagaa tcccttctcc aggtcactgt tctggtcgcc
     2221 gaacatggcg atgatccggt aaccgcgacg cagcgagatt tcggcccgtt ccgagttctt
     2281 gacctcgccc ttgctttccg aatccggcgg gcgcatgtag aggtcggtca ggccggtgta
     2341 gccctcgtcc ttcaggttcg cccaggtcac cttggtgaag ttgaaggtgt tgatgtccag
     2401 gccgcaggcg ccggtccagg ccagtgagct gctgcagagg tcggccgaac ccatcagcgc
     2461 accgaccagc ggatcggccg aaatcggctc gtagcgggcg gtgatcacga aggtcgccat
     2521 gccgcgcttc cgggccagtt tgaacaggtt gagcatcggc ttcagagccg tctgggagcc
     2581 gaggatctcg ttgcggtaca tcgagatcgt gtcgtagttc ggctgcggcg agccgtaccg
     2641 gaagctggac atcgcggtct cgtccatgtc catcgcgatc gccagtttct tcatcccggc
     2701 gcaggccttg cgggtgattt ttctcaccgg cttggtcggt tcgagggtgg aattgtccgg
     2761 caacagcgtc accgcctgcc cgtccggacc ggggtccggc gccgcgacga tgggggcagt
     2821 cggctgatag ttcgccggaa ccgcgaccgg caccgtgtaa tcggcagccg gatccttccc
     2881 cgggtccgcc ttcccgatcg ggaaatgagc cttccggcag gcgcgcacct tgccgatcgt
     2941 cggttgggtc cgtttcacta tccagcggcg acctgcctgg gccacgaagg cgaggtcatt
     3001 cgcgtacttg ccggagtcga aatactcctt gcgctgctgc ggcgtgtagg tgtccgccga
     3061 ggcagcgggg gccagggcga agccgagtgc gaggaagagg gcgagggcgg caatgattga
     3121 ccggaatccg gcaagtgaat tcttcatgcc atgaaaacta cttggcaagc cgaatgttgc
     3181 ggtttacgac tggtgaagcg ggctgccggt cagaggcttc cggatcagga accgggccgg
     3241 tctggcatct ggatccgggc catcgccttg accatgaacc agaagagcac cgagagtccg
     3301 gcggtcagga tgagggtgcc gatgatctgc atcagggtcg gcgcggtgac catgccgaga
     3361 acgccggcca ggatcagcag gacgagcagc atctggaagc cgagcaccgc ccagtacttc
     3421 gccttccaca tcccccacgc catggccgag atgaccagcg cgaaaaaggc ccactgaaag
     3481 acgttcactt cacggcctcc ggcttcggcc ccggtgaaaa gcgcgtacag ggtcgagccc
     3541 cagagcagca ccgcgaccac cgcggagaag atcgccccga ccgtgaccac ggtcggacgc
     3601 tcgccctcga ccagcggctg cagggcctcg cgggcggcct ggtttttgac ttccgcctcg
     3661 gcatagcccg ccttcatggc ctcggccccg gattgcccgg cgggccggcc gttgccgttg
     3721 gccgaaggca ccccggccgc ctcgtccgcg acccgcttcg accctctggc cttgcggcgg
     3781 gccggccgtc gcggctcctg ctcagccacc gggcaggact tccatgccga ccgaggcgaa
     3841 agagcggcgc aggctggccg cgaccgcggt cgggtcggcc gcatcgtgga ttgcccggat
     3901 cacgcagatg cggcgcgccc cggcctgaag cacctggggc acgttgtcgg ggtcgattcc
     3961 gccgatcgcg aagaagggga tcgtggtggt ggaggcggca tgccggatca gctcgagccc
     4021 ggtcgctggc cgaccgggct tggtcggggt ttcgttgatc gggccgacgc tgatgtggtc
     4081 gatcggagtg ccgtccctga cggtctggtg ggccgccgcg atctgctcct cggagtgggt
     4141 cgaaagtccg agcaggtggt tgggcccgat gatctcgcgg gcctcggccg gggatatgtc
     4201 gtcctggccg atgtggactc cgtcggcccg gaccgacatc gccagttcgg ggtcgtcgtt
     4261 gaggatgaaa gggaccccgt aggtgtcgca gacccgccgg aaggtgtcgg ccgcccgttc
     4321 gatcaccctg gtgtcggctt ccttctcgcg cagctgcacg atgtccacgc cgccgtcgag
     4381 cgccgccttc agcagcggct ccggatcgtt tccgttcggg gcggcgtcac agacgaagta
     4441 gagccgggca gtccgcaggc gttcccgccg aagtccgccg gcaccgtcag ctggttcgaa
     4501 gctcatgggc tgaggataga ggcagcaagc cggggaccgc tagcatgcgt ttacctgcgg
     4561 gagcccgatg tcccgggctg agagggtggc gacgaagcca ccgaccgcct gaacctgatc
     4621 cgggtaattc cggcggcagg gatgggcagc ggttccggtg ggaagcaaat gaaccgagct
     4681 gagccgagag aagaaagcga cgtgatcgtg gtcggggccg gcctgatcgg actcgcctgt
     4741 gcctgggagc tggccggttc cggggtttcg gtcacggtgt tcgaaagaga cgaaccgggc
     4801 tgcggtgcga cccgggtcgc ggccgggatg ctcgctccga tcggtgagct cgacttcggc
     4861 gagcccgacc tgctcgccct gaacttgcgc tcccgccggc tctatcggga gttcaccggc
     4921 gaggtcgagc gggcgaccgg tctcgacacc ggctatcgcc acaccggcgc cctgcatgtc
     4981 gcactcgacc gggacgaggc gggggagctg aaacgggaaa tggagctgca gcggtcccac
     5041 gggcttgaat cgaagtggct cgggccgatc gccgcccgcg acctcgagcc cggagtcagc
     5101 ccctcgctgg ccggcgcggt tctggtcgag gaggacgcgg tggtggatcc ccgggcgctg
     5161 gtcgaagcgc tcagggcagg ccttgcgcag aggggcgttg agatcgtgag cgccgaggta
     5221 accgggctgg aacgggattc ggccggaagg gtgaggggag tcgggacttc gatcgggtcc
     5281 ttcgccgcgg caacggtggt ggccgctccc ggtgccgtcg ccggggtcgc cgggtggctg
     5341 ccggaagaag tccggccccc ggtgcgtccg gtcaaggggc agacggtcga gctgcgcggc
     5401 ccgagcgaag atccggtctg cgagcggatt ctcgggtcgg aacgggtcta catcgcgccc
     5461 cgccctgacg gcagggtcat cctcggcgcg acctccgagg agatgggctt cgacacccgg
     5521 gtcaccgggg gcggggttca cgaactgctg cgtgaggcct atcggctgct acccgacgtg
     5581 gcggagatgg aattcgtcgg cgcggtcgcc gggctgaggc cgggcacccc ggacaacctg
     5641 ccgataatcg gccggaccgg gatcgagggc ctgatcctcg cgaccggcca ctaccgcaac
     5701 gggatcctgc tcgccccggt gacggccgag gcaatcgccg cgctggttgc cggtgatcaa
     5761 cccgagtggc tggaggcggc cagtccgggc cggttcacaa aacaggaggt gggttgatgc
     5821 gaatcgagtt gaacggaacc ccgaccgacg t
//
LOCUS       DAOQKN010000156         5815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_7189_length_5815_cov_5.010764, whole genome shotgun sequence.
ACCESSION   DAOQKN010000156 DAOQKN010000000
VERSION     DAOQKN010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5815
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7189_length_5815_cov_5.010764"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="PLJ64_09785"
     CDS             <1..871
                     /locus_tag="PLJ64_09785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HNC93814.1"
                     /translation="IKVLLDVDATPYFSGARWACGRKLKPRLTYGAALGRNGIFLRGA
                     GGKPLKKCGGRLRTEASGNVVFKGLGAYRGGVEIVRGGGSLYLINNVPLNKYCRGVLA
                     GEVIPSWPLETLKAFALAARSIGLSSKGTHDGFDVYPDTRSQVYGGISTEYPRTNRAC
                     AKTPSQALFYGGKVAMALFSASSGGHTESIQNMWGGAAIPYLQGVADPWDKVSPYYRW
                     TYSFSPARMNSLLGSYLAGRLRSVKITQTGVSKRVMWARLYGTRGVTKIRGDSLQYAL
                     GLPDRLIFSIRKK"
     gene            complement(957..1493)
                     /locus_tag="PLJ64_09790"
     CDS             complement(957..1493)
                     /locus_tag="PLJ64_09790"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNC93815.1"
                     /translation="MKQLDLKLLRIMRTRGHAAPVEKSAHLISKSGENGYLWFGIGLA
                     GAVFDGEHRKEWLLASLLGPVAIGLNFLVKLVVRRPRPELEGLPPLGGAPSSLSFPSA
                     HATASFAAATAMSRIRPELKLALFGAAAVMAVSRPYLGMHYPSDIAAGAVLGTVLGTA
                     AGNFLAGPEEDENSPYNF"
     gene            1534..2616
                     /locus_tag="PLJ64_09795"
     CDS             1534..2616
                     /locus_tag="PLJ64_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93816.1"
                     /translation="MDRPEPQTEEEFEREFRRAGLPLLNENYSASEDIFNRAVPLLGL
                     VLVLEVTGPLNKSWSTLGNVAAVATAMLLMLVALGLMNRMRGRAFFSLPRKVGRPELA
                     AFVIVPALLPGIISGHWGIAALTALFNLALLLVILGVVGFGVISIIHWALARLLGELS
                     RSLGLLVKAVPLLMIFSLILFLTPELWQVFATLPDQSLLLLVALFMLLGSAFLIARIP
                     HEVASLEKEAGGGGPPLRQRQRFNVGLVLFVSQSLQVLLVALAVAVFFVIFGLLTIGA
                     DTVSSWITHPADVLFEFHLAGREAAMTRELLRVAVAIASFTGLYFAISMLTDDLYRRE
                     FLNRITDEMKQTFVRRAEYLKLLGKG"
     gene            complement(2613..2828)
                     /locus_tag="PLJ64_09800"
     CDS             complement(2613..2828)
                     /locus_tag="PLJ64_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93817.1"
                     /translation="MAAGKTSVEIGFDGGQVIPARLDESQVKSLKKALGSGDSPHEIE
                     TDEGTLLIDLGKVIFVRISSDAKTVGF"
     gene            complement(2828..3238)
                     /gene="mce"
                     /locus_tag="PLJ64_09805"
     CDS             complement(2828..3238)
                     /gene="mce"
                     /locus_tag="PLJ64_09805"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HNC93818.1"
                     /translation="MFTRIDHIGVAVEDLDAAIKLYERNFEMELVHRETVESQGVEAV
                     LLDVGDGHVELLSALGPDTPVGKYIAKKGPGLHHVAYAVEDIDATLASLKEQGVKLID
                     QEARTGIRQSRVAFLHPAATGSVLTEIVQPAEDH"
     gene            3303..3692
                     /locus_tag="PLJ64_09810"
     CDS             3303..3692
                     /locus_tag="PLJ64_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93819.1"
                     /translation="MEQKVEGSTETRGSERLGWKGLFTNHGPKFEVSRLVHQIVFGLF
                     LILGFVPVFLMSSHIHYPWNASFLEELAPMIVFMVYGAGLFSGAGRFTRWLFGPMPKR
                     EPYPTSPLPPQYSGQDQVSKETKPKDE"
     gene            complement(3889..5181)
                     /locus_tag="PLJ64_09815"
     CDS             complement(3889..5181)
                     /locus_tag="PLJ64_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNC93820.1"
                     /translation="MRSTDLLQELIRFNTVNPPGNEGPALEHIMSFLDPAGWDCKFLA
                     KVPERPNLVARLKGVEPGPNLALISHVDTVPADPEEWSHDPWSGNLADGYIWGRGALD
                     MKDQVAAEVAACVNLARSGWRPARGDLILVVAADEETGAHFGAKWLCEEHPDEVRSDW
                     VLNEGAGELLEFDGRRLFTLSIGEKGTFRFTVITEGEAGHGSIPRVGDNALLKLAPAL
                     SNAHRQPPHESTPDTDLFLERLLGHPVEDTDAALREIEAIDPLVAKLLADPMLGVTIA
                     PTMASASSRENVIPSRAELVIDCRTPPGMTEEQVRARIDLVLGDGDWRIEFNDPVVGN
                     RSDYDGPLAEAIEEWVGLTEPGAEVLPQVMPGFSDSHWFRKAFGATVFGFAPQRMSIA
                     ETKPLVHGKDERITVEDVELMENLYTWIPPRLLGTAES"
     gene            5266..>5815
                     /locus_tag="PLJ64_09820"
     CDS             5266..>5815
                     /locus_tag="PLJ64_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93821.1"
                     /translation="MNSDGNRKATGHRIRAIGALLLALFAIGHFTSEASAGTRPLKTG
                     VTVIDFTNTSEAGYKRIRSTGATLTRVIITWPLVVGSKAPANPSDPADPAYSWGHYDT
                     QIQNAVNAGLTVLVQINGAPKWAERCKTQSTTATCNPNPVAFARFARAAATRYSGTYE
                     GLPRIRYWEPLNEPNLESNFLPQ"
BASE COUNT          984 a         1897 c         1863 g         1071 t
ORIGIN      
        1 catcaaggtc ctgcttgacg ttgacgcgac cccgtacttc agtggcgccc gctgggcctg
       61 cggccgcaag ctgaagccac gcctcaccta cggggcggcg ctcggccgca acggcatctt
      121 tctccggggc gccggtggca agccgttgaa gaagtgcggc ggccggctcc ggaccgaggc
      181 cagcggcaac gtcgtcttca aggggctcgg cgcctaccgc ggcggggtcg agatagtacg
      241 cggcggcggt tcgctctacc tgatcaacaa cgtccctttg aacaagtact gccggggtgt
      301 gctggccggc gaggtgatcc cctcctggcc gctggaaacc ctcaaggctt tcgccctggc
      361 cgcccgctcg atcggccttt cctcgaaggg aacccatgac ggattcgacg tctatcccga
      421 cacccgcagc caggtctacg gcgggatctc gaccgagtac ccgcggacca accgggcctg
      481 cgcgaagacc ccgagccagg ccctctttta cggcggcaag gttgcgatgg ccctcttctc
      541 tgcctcttcc ggtggtcaca ccgagagcat ccagaacatg tggggcggcg cggcgatccc
      601 gtacctgcag ggcgtcgccg acccctggga caaggtttcg ccgtactacc gctggaccta
      661 cagcttctcg ccggcccgga tgaactccct gctcggcagt tacctggccg ggcgcctccg
      721 ctcggtgaag atcacccaga ccggggtttc gaagcgggtc atgtgggccc gcctctacgg
      781 cacccggggc gttacgaaga tcaggggcga ttccctccag tacgccctcg gactccccga
      841 ccgactgatc ttctcgatca ggaagaagta gcgacccggg cggtcacgcc tcggattcat
      901 cgacatccca ctttgcgcca cataccggca caaagtgggc attcggtgat ccgtggctag
      961 aagttgtaag gcgagttttc gtcttcttcg gggccggcga ggaagtttcc ggcggcggtt
     1021 ccgaggactg tgccaagaac cgccccagcg gcgatgtcgc tggggtagtg catgccgagg
     1081 tagggacggc tgaccgccat cacggcggcg gctccgaaga gggcgagttt caattccggg
     1141 cggatccggc tcatcgccgt agccgcggca aacgaagccg tggcatgggc ggaggggaac
     1201 gaaagcgaag agggcgcacc gcccagcggg ggcaggcctt caagctccgg ccgcggacgc
     1261 cttaccacca gcttgaccag gaagttaagg ccgatcgcca ccgggccgag cagcgaagca
     1321 agcagccact ccttgcggtg ctctccgtca aaaaccgcgc cggccaggcc gatcccgaac
     1381 cagaggtagc cgttctcgcc cgacttcgag atcaggtgag ccgacttctc gaccggagcg
     1441 gcgtgaccgc gggtgcgcat gatgcgcagc agtttcaaat ccagttgctt cacgaagtga
     1501 gcagaatagg gggccattcg ggatgatcgc ggcgtggatc gtcccgaacc ccagactgaa
     1561 gaagagttcg agcgggaatt ccgccgcgcc ggcctgccgc tcctgaacga gaattactcg
     1621 gcttcggagg acatcttcaa ccgggccgtg ccgctgcttg gactggtcct ggtcctggaa
     1681 gtgaccggac ctctgaacaa gagctggtcg acccttggca acgtcgccgc ggtggcgacc
     1741 gcgatgctgt tgatgctggt cgctctcggc ctgatgaacc ggatgcgcgg ccgcgccttc
     1801 ttctcgctgc cgcgcaaagt gggacgcccc gagctcgccg ccttcgtaat cgtgccggcc
     1861 ctcctgccgg gaatcatttc cggccactgg ggaatcgcgg ccctgacggc cctcttcaac
     1921 cttgccctgc tcctcgtcat cctcggagtc gtcggtttcg gcgtgatctc gatcatccac
     1981 tgggccctgg cccgcctgct cggggagctt tcccgttcgc tcgggctgct ggtcaaggca
     2041 gtgccgctct tgatgatctt ctccctgatc ctcttcctga ccccggagct ctggcaggtc
     2101 ttcgcgaccc tgcccgatca gtcgctcctg ctgctggtcg ccctcttcat gttgctcggc
     2161 tcggccttcc tgatcgcccg gattccccat gaggtggcca gcctcgaaaa ggaggcgggc
     2221 gggggaggcc cgccgctcag gcagcggcag cggttcaacg tcgggctggt cctcttcgtc
     2281 agccaatcac tccaggtgct gctggtggcc ctcgcggtcg ctgtcttctt cgtgatcttc
     2341 ggcctcctga cgatcggcgc cgacacggtg agcagctgga tcactcaccc ggccgacgtt
     2401 ctcttcgagt tccacctggc cgggcgcgag gcggcgatga ccagggaact gctccgggtc
     2461 gcggtcgcga tcgcttcctt caccggcctt tactttgcga tctcgatgct gaccgacgac
     2521 ctctaccgcc gggagttcct caaccggatc accgacgaga tgaagcagac cttcgtccgc
     2581 cgggccgagt acctgaaatt gctcgggaag ggctagaagc cgacggtctt ggcgtccgag
     2641 gagatgcgga cgaagatgac cttgccgagg tcgatcagca gggttccctc gtcggtctcg
     2701 atctcatgcg ggctgtcccc ggagccgagc gccttcttca gtgacttgac ctgcgattcg
     2761 tcgaggcggg ccgggatcac ctggccaccg tcgaaaccga tctcgaccga agtcttgccg
     2821 gcggccatca gtgatcctcc gccggctgga cgatttcggt caggaccgag ccggtcgcgg
     2881 ccgggtgcag gaaggcaacc cggctctggc ggatgccggt gcgggcttcc tggtcgatca
     2941 gcttcacgcc ctgctccttg agcgaggcga gcgtggcgtc gatgtcttcg accgcatagg
     3001 cgacatggtg gaggccggga cccttcttcg cgatgtactt gccgaccggg gtatcggggc
     3061 cgagggcgct caacagttcg acgtgcccgt cgccgacgtc gagcaggacc gcctcgactc
     3121 cctgcgattc gacggtttcg cggtgaacga gctccatctc gaaattccgc tcataaagct
     3181 tgatggcggc gtcgagatcc tcaacagcca caccgatgtg atctatgcga gtgaacatga
     3241 ggcgacaagc ctagcgatga ggcctatccc accatccggc cgaggccgga tatcctccgg
     3301 ccatggagca gaaagtcgaa ggatcgactg agaccagggg gagcgaaagg ctcggctgga
     3361 agggcctctt cacgaatcat ggcccaaagt ttgaggtgtc ccggctcgtc caccagatcg
     3421 tatttggatt gtttctgatc ctcgggttcg tcccggtctt tttgatgtcg agccacatcc
     3481 actacccgtg gaacgcatct tttctcgaag agctcgcgcc gatgattgtc ttcatggtct
     3541 acggcgctgg cttgttctcc ggcgctggcc ggttcacccg gtggcttttt gggccgatgc
     3601 ccaagcgcga gccgtacccc acatcaccgt tgccacctca gtattcggga caggatcagg
     3661 tttccaagga aacgaaaccg aaagacgaat gaattaccaa ccgtcaacag atgaattccg
     3721 cccgttgttt ctcgttcact gcctcactgg cgagcttgtc ggtcgcgaat ctcgaccttg
     3781 gcagctgagg gtcgacccgg ccgatcgcag gccccgtcga cccaaaccgt cctaatccac
     3841 ggtttcccgc agcgaaaatc ttgtggggcg tgatcggcct gggccgggtc aggactccgc
     3901 ggtcccgagc aggcggggtg ggatccaggt gtagaggttt tccatcagct ctacgtcctc
     3961 gacggtgatt cgttcgtctt tgccgtggac gaggggcttg gtttcggcga tgctcatgcg
     4021 ctggggggcg aagccgaaga cggtggcgcc gaaggccttg cggaaccagt gggaatccga
     4081 gaagccgggc atgacctggg gcaggacctc ggcccctggt tcggtcaacc cgacccactc
     4141 ctcgatcgcc tcggccagcg gtccgtcgta atcggagcga ttgccgacca cggggtcgtt
     4201 gaactcgatc cgccagtcgc cgtcaccgag tacgaggtcg atccgggccc ggacctgctc
     4261 ttcggtcatg ccgggcgggg tgcggcagtc gatcaccagc tcggcccggg aggggatcac
     4321 gttctcgcgg ctggaagcgc tcgccatggt cggggcgatc gtcactccga gcatcgggtc
     4381 ggccaaaagc ttggcgacca gcgggtcgat cgcttcgatc tcgcgcaggg cggcgtcggt
     4441 gtcctcgacc ggatggccga gcagcctttc gaggaagagg tcggtgtccg gggtcgactc
     4501 atgcggcggc tggcgatggg cgttcgaaag cgcgggggcg agcttgagca gggcgttgtc
     4561 accgacccgg gggatcgaac cgtggccggc ctccccttcg gtgatcacgg tgaagcggaa
     4621 agtgcccttc tcgccgatcg agagggtgaa gagccggcga ccgtcgaatt cgagcagttc
     4681 gccggcgcct tcgttcagga cccagtccga gcggacctca tccgggtgct cctcgcagag
     4741 ccacttggcc ccgaaatggg cgccggtctc ctcgtcggcg gcgacgacca ggatcaggtc
     4801 accacgggcc ggccgccatc cggacctagc caggttgacg caggcggcga cctcggccgc
     4861 gacctgatct ttcatgtcga gcgcaccgcg gccccatatg tatccgtcgg cgagattgcc
     4921 agaccagggg tcatgcgacc actcctccgg atcggccggg acggtgtcca catgactgat
     4981 cagggcgagg ttgggtcccg gttcgacgcc cttgagccgc gccaccaggt tgggccgctc
     5041 cggcaccttc gccaggaact tgcagtccca gccggccggg tcgagaaagc tcatgatgtg
     5101 ttccagcgcc gggccctcgt tcccgggcgg attcaccgtg ttgaagcgga tcagctcctg
     5161 aagcaggtcg gtcgagcgca taggcgcagg atctcacagc ggcggtactt atccggtcga
     5221 cgtggtttat agatcccgat gcctgttgaa ataatcccca gtcccatgaa ctccgatgga
     5281 aacaggaagg ccaccggaca ccggatccgc gcgatcggtg cgctcctgct cgcccttttc
     5341 gcgatcggtc atttcacgag cgaggcctcg gccggaaccc gtccgctgaa gaccggagtc
     5401 acggtgatcg acttcaccaa tacgagcgaa gccggctaca agcggattcg cagcaccggt
     5461 gccaccctga cccgggtgat catcacctgg ccgctcgtgg tcggctcgaa agctccggca
     5521 aatccgtctg acccggccga cccggcctac agctgggggc actacgacac ccagatccag
     5581 aacgcggtga acgccgggct gacggtgctg gtccagatca atggggcgcc gaaatgggcc
     5641 gaacgctgca agacccagag caccacggct acctgcaatc cgaatccggt cgccttcgct
     5701 cgttttgcca gggcggcagc gacccgatac agcggcacct atgaagggct gccccggatc
     5761 cgctactggg agccacttaa cgagcccaac ctcgaatcga actttctgcc gcaga
//
LOCUS       DAOQKN010000157         5806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_7206_length_5806_cov_5.834811, whole genome shotgun sequence.
ACCESSION   DAOQKN010000157 DAOQKN010000000
VERSION     DAOQKN010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5806
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7206_length_5806_cov_5.834811"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="PLJ64_09825"
     CDS             complement(<1..986)
                     /locus_tag="PLJ64_09825"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNC93822.1"
                     /translation="MSKFGGREVVIVEAVRTPVGRGHKEKGYYKDTHPSTLLAHAYKN
                     VVERAGIDPGEVEDVVAGCVQQFGEQTLNIGRNAWLEAGFPIETPATTIDRQCGSAQQ
                     AINYAAAMIASGVHDVMIGGGVEHMGHISFAHSAKVMSEYGYAYSPELLEKYDLVNQG
                     ISAEMIADQWEIPKVDLDELAVRSHARAQQATEEGRFEREIAPFQVNGDTYMTDQGIR
                     PGTNLEALAGLKPAFKEDGKITAGNSSQVSDGAAAVLLMTKEKADELGLKARARIVDQ
                     TAVGVDPVIMLTGPIPATRKILERNDMTMDDIDLTEINEAFASVVKAWEIEL"
     gene            1108..1935
                     /locus_tag="PLJ64_09830"
     CDS             1108..1935
                     /locus_tag="PLJ64_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNC93823.1"
                     /translation="MTATPYEFVAIAGSGTIATGLAAVASTSSGKVVMLVRSEESADR
                     ALTAVEKASRRIPGANPSRVTTTMEIADLAEANLLVEAVVEDAGVKADLLRRISEVAP
                     RADLATTTSSLSITKLGEDSGAADRVYGLHVFNPVPAMQLVELIFPDPVTEEVAERAH
                     DWCHILEKTAVEVPDTAGFAVNRLLFPYLFDAVRYQERTGLEAAAVDQCMTLGVAHPM
                     GPLALLDLVGLDVAVAIGQALNNESGNPDHIAPATVQEFVAAGHLGRKTGQGFYEYG"
     gene            complement(1932..2210)
                     /locus_tag="PLJ64_09835"
     CDS             complement(1932..2210)
                     /locus_tag="PLJ64_09835"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HNC93824.1"
                     /translation="MALLEDQEIQDRLAELDGWSLEGRRIVRTFEFDDFVGSINFVDS
                     LVQPAESMNHHPDLGISWNKVTVAITNHAEGGLTEKDFELASRIQALV"
     gene            2245..3159
                     /locus_tag="PLJ64_09840"
     CDS             2245..3159
                     /locus_tag="PLJ64_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HNC93825.1"
                     /translation="MRIASLVPSSTEMLFALGFGDQVVAVTHECDFPPEAAALPHLSE
                     TVIEPGLSPAEIDAEVKRVVGQGDPLYRLKAEVLESVRPDLIIAQDVCAVCAVSYDDV
                     LAIAARLPGNPVVLKQDPTSLVEVLGNAIEVAEATGNPEAGVALKAELDARIESVRSA
                     VSGKERPTVLALEWLDPPFTGGHWVPEMIEIAGGRDLVGKAGLKSAEHGWDELANLDP
                     DLTVVMPCGYYVDEAATQADDYMDRIRSTGAERTVAVDAAASFSRPGPRLIDGIELLA
                     GLLHPEVFPLEEGQIQYREMQTGDRFGG"
     gene            complement(3100..3969)
                     /locus_tag="PLJ64_09845"
     CDS             complement(3100..3969)
                     /locus_tag="PLJ64_09845"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNC93826.1"
                     /translation="MATCYRHPNVETGVSCSSCGRPICPDCMTTTPVGMRCPECATEK
                     TRVRSGPAATSQVGGYPATIALIAINVVVFLAEMATGSGGLSSTSSDLISDFGLRGAQ
                     VADGETYRLVTSGFLHAGFMHIAFNMIALYFLGRVLEPSIGTVRFLMVYFASLLAGSF
                     GAVLLSDNLALTVGASGAVFGLFAATFIIAYGRGLGHVANEIGLIIGINLLLTFTISG
                     ISIGGHLGGLIGGGLCGILVIAGERGYLGSNARLLEYGGMIAIAAVSVFAAISAAEPV
                     PGLHFAVLNLPFL"
     gene            4027..4533
                     /locus_tag="PLJ64_09850"
     CDS             4027..4533
                     /locus_tag="PLJ64_09850"
                     /inference="COORDINATES: protein motif:HMM:NF025683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HNC93827.1"
                     /translation="MERTAYARLYATVTGAFLVLLGFVSLLSNTEFQARELTSELLGF
                     YAINGWSGVFHVAAGLLGLLLARPLPRLYALLAGIVFTGLGIWGILAANGTWLLGGLP
                     ANRWANLVNLLIGLLGIIAYAASRWDRITAFTGSLGSRFEKMAENRRQKRRRRKIRKR
                     RTATKSRA"
     gene            complement(4566..4847)
                     /locus_tag="PLJ64_09855"
     CDS             complement(4566..4847)
                     /locus_tag="PLJ64_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93828.1"
                     /translation="MDEIRIENLLADALKPVEPPQRMSDRLHDTFSAITDAAASDLSD
                     WAEELTESELESLRDPRNWIRPVTALAAGGVATGALILVGFRRRSRYRG"
     gene            complement(4840..5406)
                     /locus_tag="PLJ64_09860"
     CDS             complement(4840..5406)
                     /locus_tag="PLJ64_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNC93829.1"
                     /translation="MSGPDAKSTPESKAEIDRAFEELYRDHLKDVYSYCYYRIGNHHD
                     AEDLTEQTFLQAYRHFARARRESDGRPLRPWLIRIAHNLAANYYRDRSRKPQTPLESI
                     EPPAAKHSTEDLVEGRAELRAVIENLQDLPDDRREALVMRFALGMDNREIARAMGKSD
                     GATKVLIHRAIKQLQESLEAEGVSVEHG"
     gene            complement(5452..>5806)
                     /locus_tag="PLJ64_09865"
     CDS             complement(5452..>5806)
                     /locus_tag="PLJ64_09865"
                     /inference="COORDINATES: protein motif:HMM:NF019122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HNC93830.1"
                     /translation="AVWDECVGYAVKHGADLETFNGLAGVGDLLATVMASGSRNRRAG
                     ELLGKGSAPDQIRETIGQASEALDSVPQIAATVAADGCPAEALTGLADLATGRISSDE
                     WVAALRRSGHSRRAA"
BASE COUNT         1021 a         1885 c         1859 g         1041 t
ORIGIN      
        1 tggagctcga tctcccaggc cttgaccacc gaggcgaagg cctcattgat ctcggtcagg
       61 tcgatgtcat ccatcgtcat gtcgttgcgc tcgaggatct tgcgggttgc cgggatcggc
      121 ccggtcagca tgatcaccgg gtcgacaccg accgcggtct gatcgacgat ccgggcgcgg
      181 gccttgagcc cgagctcgtc tgccttctcc ttcgtcatca gcagaaccgc ggcagcgccg
      241 tcggagacct gtgaggagtt gccggcggtg atcttgccgt cttccttgaa ggcgggcttg
      301 aggccggcca gcgcctcgag gttggtgccg ggacggatgc cctggtcggt catgtaggtg
      361 tcaccgttga cctggaaggg ggcgatttcc cgttcgaagc gaccttcttc ggtcgcctgc
      421 tgggcgcggg cgtgggaacg aacggccagc tcgtcgagat cgaccttggg gatctcccac
      481 tggtcggcga tcatctcggc cgagatgccc tggttgacca ggtcgtactt ctcgagcagc
      541 tcgggcgaat aggcgtagcc gtattcgctc atcaccttgg ccgaatgtgc gaaggagatg
      601 tggcccatgt gctcgacacc gccgccgatc atcacgtcgt ggacgccgga agcgatcatc
      661 gccgctgcgt agttgatcgc ctgctgggcg gagccgcact gacggtcgat cgtggtcgcc
      721 ggggtctcga tcgggaagcc ggcctcaagc caggcgttgc gtccgatgtt cagggtctgc
      781 tcgccgaact gctgcacgca accggcaact acatcctcga cctcgcccgg gtcgatcccg
      841 gcccgctcga cgacgttctt gtaggcatgg gccagaagag tcgaaggatg ggtgtccttg
      901 taatagccct tctccttgtg gccgcggccg accggggtgc ggaccgcctc cacgattacg
      961 acttcacgac cgccgaactt gctcattttc tgcctttctt ctcctgattg atggctggaa
     1021 agaggggttg caaacttgta acaatcgtag ctgattgact cgtcagtcaa tcccggttgt
     1081 tggtcgatct caatagggtt cagccggatg accgcaacac cgtatgaatt cgtcgcgatc
     1141 gcgggcagtg gcacgatcgc caccggcctc gcggccgtgg catcgacctc gagcggcaag
     1201 gtcgtgatgc tggttcgttc cgaagagtcg gcggaccggg ccctgaccgc cgtcgaaaag
     1261 gccagccgga gaatccccgg agccaacccc tcacgggtga ccacgaccat ggagatcgcg
     1321 gacctggctg aagccaacct cctggtcgaa gcagttgtcg aggacgccgg ggtcaaggcg
     1381 gatctgctga gacgaatttc cgaggtcgcc ccgcgggccg atctcgcaac caccacttcc
     1441 tccctttcga tcacgaagct gggcgaggat tcgggggccg ccgaccgggt ctacggactc
     1501 catgtcttca accccgtccc ggcgatgcaa ctggtcgaac tgatctttcc cgacccggtt
     1561 accgaggagg tagccgagcg agcccacgac tggtgccaca tcctcgaaaa gaccgccgtc
     1621 gaggtgcccg acacagccgg tttcgcggtc aaccgtctgc tcttccccta cctcttcgac
     1681 gcagtccgct accaggagcg caccggcctg gaggctgccg cggtcgacca atgcatgacc
     1741 ctcggggtcg cccaccccat gggccccctc gccctgctcg acctggtcgg cctcgatgtc
     1801 gcggtagcga tcggccaggc actcaacaac gaatcaggaa acccggacca catcgccccc
     1861 gccacggtcc aggaattcgt cgctgcaggc cacctcggcc gcaaaaccgg ccagggtttc
     1921 tacgaatacg gctaaaccag cgcctggatc cttgaggcca gctcgaagtc tttctcggtg
     1981 aggccgcctt cggcgtggtt ggttatcgcc acggtgacct tgttccagga gataccgagg
     2041 tcggggtgat ggttcatcga ttcggccggc tgaaccagcg aatcgacgaa gttgatcgag
     2101 ccgacaaagt cgtcgaactc gaacgttcga acgatcctcc ggccctcgag gctccagcca
     2161 tcgagttcag ccaggcggtc ctgtatttcc tgatcctcaa gcagcgccat ggtccgagcc
     2221 tacccgggct gccatatttc acccatgagg attgcctccc tcgttccttc ttccaccgag
     2281 atgctcttcg cgctcggctt cggcgaccag gtcgtggccg tcacccatga gtgcgacttc
     2341 ccgcctgagg ctgccgctct cccccacctc agcgagacgg ttatcgagcc gggcctttcc
     2401 ccggccgaga tcgacgccga agtgaagcgg gtggtcggcc agggtgaccc gctctaccgc
     2461 ctcaaggccg aggtactgga atcggtccgg ccggacttga tcatcgccca ggatgtctgc
     2521 gcggtctgcg ccgtctctta cgacgatgtg ctggcgatcg ccgcccgcct gcccggcaat
     2581 ccggtggttc tcaagcagga cccgacctcc ctggtggagg tcctgggcaa cgcgatcgaa
     2641 gtggcggaag cgaccggcaa ccccgaagcc ggcgttgccc tcaaggccga actcgatgca
     2701 aggatcgaat cggttcgcag cgcagtgtcc ggcaaggaac gaccaaccgt gctggccctc
     2761 gagtggctgg acccgccctt caccggcggc cactgggtgc cggaaatgat cgagatcgcc
     2821 ggcggccgcg acctggtcgg caaagcaggc ctgaagtcag ctgaacatgg atgggacgaa
     2881 ctggccaacc tcgatccgga cctgaccgtg gtcatgccct gcggctacta cgtcgatgag
     2941 gccgccaccc aggccgacga ctacatggac cggatcaggt caaccggagc agagcggacc
     3001 gtcgcggttg acgccgccgc ctcgttctca aggccaggcc ctcgcctcat cgacggaatt
     3061 gaattgctgg cgggtctgtt gcatcccgag gtttttcctc tagaggaagg gcagattcag
     3121 taccgcgaaa tgcagaccgg ggaccggttc ggcggctgag atcgcggcga ataccgaaac
     3181 tgcggcgatc gcgatcatcc ctccgtactc gagcaggcgg gcattcgaac ccaggtaacc
     3241 acgctcgccg gcgatgacca gaattccgca caacccgccg ccgatcagac caccgagatg
     3301 gccgccgatg ctgatgccgc tgatcgtgaa ggtgaggagc aggttgatac cgatgatcaa
     3361 tccgatctcg ttcgccacat ggccgagccc acggccgtag gcgatgatga aagtcgccgc
     3421 gaagagcccg aagacggcgc cggaggcacc gacggtcagg gcgaggttgt cgctgagcag
     3481 gacggccccg aaagaaccgg ccagaagcga cgcgaagtag accatcagga agcgcaccgt
     3541 gccgatcgaa ggctcgagta cccgtcccag gaagtaaagg gcgatcatgt tgaaggcgat
     3601 gtgcatgaag ccggcgtgga gaaaaccgct ggtgaccagc cggtaagtct cgccgtcggc
     3661 cacctgtgcg ccgcgcaggc cgaaatcgct gatcagatct gaactggtcg agctgagacc
     3721 accggatccg gtcgccattt ccgccaggaa tacgaccacg ttgatcgcga tcagggcgat
     3781 cgtggccggg tagccgccga cctgggaggt tgccgcagga ccgctgcgga cccgggtctt
     3841 ttcggtcgca cattccgggc agcgcatgcc gaccggggtc gtcgtcatgc agtcggggca
     3901 gatcggacga ccgcagctcg aacatgaaac gcctgtctcg acgttcgggt ggcggtaaca
     3961 ggtagccaag gccgtcaaac cctatcggtg tgcccgggtc cggcgttcct gtgagaatgg
     4021 cggtcgatgg agaggacggc atacgcccgg ctgtacgcga cggtgaccgg cgccttcctc
     4081 gtgctgcttg gtttcgtcag cctcctcagc aacaccgagt ttcaggcccg cgagctgacc
     4141 agcgagctgt tgggcttcta cgcgatcaac ggctggtccg gtgtctttca cgtcgccgcc
     4201 ggactactcg gcctgctgct cgcccgcccc cttcctcgcc tctacgcgct gctggccgga
     4261 atcgtcttta ccggccttgg catctggggc atcctcgccg ccaacggaac ctggctgctc
     4321 ggtgggctgc cagccaaccg gtgggcaaac ctggtcaacc tgctgatcgg cctgctcggc
     4381 atcatcgcct acgcggccag ccgctgggac cggatcaccg cgttcaccgg cagcctcgga
     4441 agccggttcg aaaagatggc ggaaaatcgc cgccagaagc gcaggcgacg caagatccgc
     4501 aaacgccgga cagcaaccaa gagccgcgcc tagccgcgga agcggctggt cacaagggtg
     4561 atgtgtcagc cgcggtagcg gcttcgacgg cggaagccga ccaggatcag ggcgccggta
     4621 gctaccccgc ccgcggcgag ggcggtgacc ggacggatcc agttgcgggg gtcacgcagc
     4681 gactcgagtt cactttcggt cagttcttcg gcccaatcgg aaaggtccga agcggcggcg
     4741 tcggtgatcg ccgagaaggt gtcgtgaagt cgatcagaca tgcgctgtgg cggctcgacc
     4801 ggcttcagcg catcggcgag caggttctcg atcctgatct catccatgct caacggaaac
     4861 tccttccgct tccagcgatt cctgcagctg cttgatcgcg cgatgaatca gcaccttggt
     4921 ggccccgtcg gatttgccca tcgcgcgggc gatttcgcgg ttgtccatgc cgagcgcgaa
     4981 acgcatgacc agagcctcgc gacgatcgtc tggcagatcc tgaaggttct cgatcaccgc
     5041 ccggagttcg gcccgaccct cgaccagatc ctcggtcgag tgcttcgcgg ccggcggctc
     5101 gatgctctcc agcggagtct gcggtttccg cgaacgatcg cggtagtagt tcgcagcgag
     5161 gttgtgggcg atccggatca gccaggggcg aagcgggcga ccgtccgatt cccgccgggc
     5221 ccgggcaaag tgccggtagg cctgaagaaa tgtctgctcg gtcagatcct cggcgtcgtg
     5281 gtgattgccg atccggtagt agcagtagga gtagacgtcc ttgaggtgat cgcggtagag
     5341 ctcctcgaag gcccgatcga tctcggcctt cgactccgga gttgatttcg cgtcggggcc
     5401 ggacacgccc gcaccttatc ccgaaaccgg cgacctccga cccgggcgtg ctcatgccgc
     5461 ccgcctcgaa tgcccggagc ggcggagcgc cgcaacccat tcatcggagc tgattcgccc
     5521 ggtggcgaga tccgcgaggc cggtcagggc ctcggcgggg cagccgtcgg ccgccacggt
     5581 ggcggcgatc tgggggaccg aatcgagagc ttccgaagcc tggccgatcg tttcgcggat
     5641 ctggtccggg gcgctgccct tgccgagcag ttcacccgcc cggcgattgc gactgccgga
     5701 tgccatcacc gtggcaagca ggtctccgac cccggcgagg ccgttgaagg tttcaaggtc
     5761 cgccccatgt tttacggcgt aaccgacgca ttcatcccag accgcg
//
LOCUS       DAOQKN010000158         5764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_7294_length_5764_cov_5.372920, whole genome shotgun sequence.
ACCESSION   DAOQKN010000158 DAOQKN010000000
VERSION     DAOQKN010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5764
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7294_length_5764_cov_5.372920"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /locus_tag="PLJ64_09870"
     CDS             complement(<1..1006)
                     /locus_tag="PLJ64_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93831.1"
                     /translation="MNQQGDPVSAEAEPKSQVDESSAAKARKRRRALPTVLIVLATVV
                     GIISVFALWAQRQLMEQDTWSNTSQQLVENGEIQSALSDFIVTSIYDNVDVEQSLADR
                     LPPDLAPIAGPVASALRGPTEKIADKALQQPKIQGLFVDASDATHQKFVQLIEDKGEF
                     VSTSGGVVTLDLKSLLTSVTSQLGVGSKAADRLPASAASIEIMKSTELSQVQTGVKAL
                     KTAAWFLTALTLVLFAAAILLGRGRRREILRSVGVSLVFIGLVVLFARNFASNQIVNS
                     LSGSAANEAAVNSAFDIITSLLRDTGQSILVYGIVVILAAWFAGPARWAVSARRAVTP
                     W"
     gene            complement(1096..1467)
                     /locus_tag="PLJ64_09875"
     CDS             complement(1096..1467)
                     /locus_tag="PLJ64_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93832.1"
                     /translation="MSSSSRLFLTCAAIVIAIVASAAPAQAKAPPRGKYDCTISGNLF
                     GVLTIKSKGRYSHRGSSGTFRAKGGSVRFPDGIVAWRISFKGGSLGGMKGRWHRTSDG
                     VDEIALKNPINGFESIYCDRR"
     gene            1559..2233
                     /locus_tag="PLJ64_09880"
     CDS             1559..2233
                     /locus_tag="PLJ64_09880"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC93833.1"
                     /translation="MDDATAGTWRPDPLPRGRHQLPADVVRASQRERLLLAMLVLVAE
                     NGYQATTVPEVVTRARVSRSVFYQEFESKLDCFLALCDEQSRGMIDQVLEAAGDFVDR
                     TWQEVIAGGMDAYLRWWRDRPEFARAFLVELPAAGTEGLEHRIDLLRPFEDLFAGLAR
                     YMRSKDRTLAPLPRLAPKSVVRGTTDLIAEEVRQGRVATLPDLTGDLVDVISVALGGA
                     PVPSRR"
     gene            complement(2190..3932)
                     /locus_tag="PLJ64_09885"
     CDS             complement(2190..3932)
                     /locus_tag="PLJ64_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93834.1"
                     /translation="MPDGKRLVTVVASPDLTAPQLAITRLDGSHPKCVTCGLDTAVVP
                     GAPVDAAFGKPAPFPDGKRLMVRLGLRPGAGGSPTQNFTYGVVECTPTLVNCEERTLS
                     PIIMPGGGIDQGTQNREGRLSPDGRWFGWTEFTMNGTAMSVGRLAKNGGQYDLENVRI
                     LNPSGPLGTDSAAWRAQSPYFELKNFTSDGKSVLYSTTYDASNFDTWRVDLRNGKRTR
                     ITRGIDWEEDVSTSPDGGSYLQFSSRGFDRMPIFALMPRPPFIEFALFSWVGRYELNQ
                     VNRKCLLEPWLLDSGGERGTYFGQPVDPRPGTGWDNRTLGQWSPDGTRLLMWQARGND
                     DGTPEKPGARIVIADLPARKATGKRSNNTTPVPGWAPARSSYTGFLANSGTFTVYGKK
                     TGSATVSMEGTAFGGSWSVTYDNYSDDGKSFLDGTESTTQASILSGITWTAELTLTGK
                     HSGHLSANLQIIKGEAGAGAQVSGTVSQSLDDRTVSEFPSATCEPLARPRLRVKAARQ
                     KAGRVRVLVTGKVTADPVWRPVRYATVRLGGERVRTDRKGIAVLKGKRGRMITASAAG
                     FTGAKARAPRPGRR"
     gene            4492..>5764
                     /locus_tag="PLJ64_09890"
     CDS             4492..>5764
                     /locus_tag="PLJ64_09890"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNC93835.1"
                     /translation="MSAGGSPVSGAVVEALDVSTSAVLASDTTGAGGSFSMTVSASSV
                     DVRVTPPSGSGLQISTIHAVDTTSNANLEIILVPTASQVQLSGTLRDEHGNPVANGIV
                     QLSSGPYVYTDAAGHYSFTMMPPGTYALYVYGDNLPGLPAHWSFYKPGVDMSADRVLD
                     LTLPSTVSLSFKVQDPDGNPISGAQVVGVSASGNAMSLGPGVTGVQGTTYMNGESVTT
                     DANGIAHYTVFGPVDSVSGAAVPNQPGFSNAGFSVYPFDQNTTTVITVPRTVQLSGTL
                     RDEHGNPVENAIVQLAGGAYVYTDASGHYSFTTTPGTFALYVYGDDLPGLPAHWSFYK
                     PGVVVNSDRVLDLTLPSTVSLSFKVQDQNGTPVAGAQLVGVSASGNHMSLGPDAPDVQ
                     GTTYMNGESVTTDGNGIAHYTVFGPVDSVSGA"
BASE COUNT          995 a         1838 c         1899 g         1032 t
ORIGIN      
        1 gccacggggt cacggcgcga cgcgcggaga ccgcccagcg ggccggtccg gcgaaccagg
       61 ctgcgaggat gaccacgatg ccgtagacga ggatcgactg gccagtgtcc ctgagaagcg
      121 aagtgatgat gtcgaaagcc gaattgacgg cagcttcgtt tgccgccgac ccgctcaggg
      181 aattgacgat ctgattcgaa gcgaagttgc gggcgaacag gacgaccagt ccgatgaaga
      241 cgagggagac gccgacgctc cggaggatct cgcgtcgacg gccgcggccg agaaggatcg
      301 ccgcagcgaa gagcacgaga gtcagcgccg tgaggaacca ggcggctgtc ttcagcgcct
      361 tgacgccggt ctgcacctgg gagagctcgg tggacttcat gatctcgatg gaagcggcgc
      421 tcgcagggag cctgtcggcc gctttggaac cgacgcccag ctgggaggtc acggatgtaa
      481 ggaggctttt caggtcaagg gtgacgactc cgcccgaggt ggagacgaac tcgcccttgt
      541 cttcgatcag ctggacgaac ttctggtggg tggcatcgct ggcgtcgaca aaaaggccct
      601 ggatcttcgg ctgctgaagg gctttgtcgg cgatcttctc ggttgggccg cgcagagcgg
      661 aggcaaccgg cccggcgatc ggagccagat ccggaggcag acggtcggcc aggctctgct
      721 cgacgtcgac gttgtcgtag atgctggtga cgatgaagtc ggaaagcgcc gactggatct
      781 caccgttctc gaccagttgc tgactggtgt tcgaccaggt gtcctgctcc atcaactgac
      841 gctgggccca gagggcaaac accgaaatga tcccgaccac ggtcgcgaga acgatcagga
      901 cggtgggcag tgcccgacgc cgtttccggg ccttcgccgc ggaactttca tctacctggg
      961 acttgggttc ggcctcggcg ctcacgggat ctccttgctg gttcacgcga agaaactaga
     1021 cgactacacg gaacttcgga cgataagccc ctcggagccc cgcctgatca ccccgatcag
     1081 gtgaagcgtc gaaggctacc ggcggtcgca gtagatgctc tcgaatccgt ttatggggtt
     1141 cttcagggcg atctcgtcaa cgccgtccga ggtccggtgc cagcgaccct tcatgccccc
     1201 gagagatccg cccttgaagc tgatcctcca agccacgatg ccgtcgggaa aacggaccga
     1261 gccgcccttc gcccgaaagg taccgctgct gccgcggtgg ctgtacctgc ccttgctctt
     1321 gatggtcagc accccgaaaa ggtttccgct gatcgtgcag tcgtacttgc cgcgaggggg
     1381 cgccttcgcc tgggccgggg cggccgaggc gacgatcgcg atcacgatcg cggcgcaggt
     1441 cagaaacagc cgtgaggacg aagacatgcc gcgacgctat cccaattccc gaaaagccgc
     1501 aacaggtcga acgaagttgc cgtggtcgga gccgggattg gctctagaat gtggcgccat
     1561 ggacgatgcg actgcgggga catggcgccc ggatcccctg ccgaggggaa ggcatcagct
     1621 gccggccgat gtcgtcaggg cctcccagcg ggaaaggctg cttctggcga tgctcgtcct
     1681 ggtggccgag aacggctacc aggcgaccac cgtgcccgag gtggtcaccc gggcccgcgt
     1741 atcgcggtct gtcttctacc aggagttcga aagcaagctg gactgcttcc tcgccctttg
     1801 tgacgagcaa tccaggggca tgatcgacca ggttctcgaa gcggccggcg acttcgtgga
     1861 tcggacatgg caggaggtga tcgcgggggg aatggacgcc tacctgcgct ggtggcggga
     1921 tcgtcccgag ttcgcccggg cgtttctggt cgagctgccg gctgccggca ccgaaggact
     1981 tgagcatcgc atcgatctgc tccggccctt cgaggacctg ttcgcgggcc tggcccgcta
     2041 catgaggtca aaggaccgga ccctggctcc gcttccccgc ctggctccga agtcggtggt
     2101 tcgcggaacc accgatctga tcgccgagga ggtcaggcag ggacgggttg cgacactgcc
     2161 ggacctgacc ggcgacctgg ttgacgtgat cagcgtcgcc ctgggcgggg cgcccgtgcc
     2221 ttcgcgccgg taaacccggc agcacttgcg gtaatcatcc ggcctcgctt ccccttcagc
     2281 acggcgatgc ccttccggtc ggttcgaacc cgctcgccac caagccgtac cgtggcgtac
     2341 cgcaccggcc gccagaccgg atcggccgtg accttgccgg taaccagcac gcgaacccgg
     2401 cccgcctttt gacgggcagc cttgacccgg agccggggcc ttgccagcgg ctcgcaggtg
     2461 gcggagggga attccgagac ggtgcggtcg tccaggctct ggctcaccgt gccggaaacc
     2521 tgggcaccag cgccggcctc ccccttgatg atctggagat tcgcgctcag gtggcccgaa
     2581 tgcttcccgg tcagagtcag ttcggcggtc caggtgattc cgctcaggat cgatgcctgg
     2641 gtggttgact cggtgccgtc gaggaaagac ttcccgtcat cggaatagtt gtcgtaggta
     2701 acgctccagc tgccgccgaa agcggtgccc tccatgctga cggtggccga tccggtcttc
     2761 ttcccgtaca cggtgaaagt gcccgaattg gcgaggaaac cggtgtagga gcttctggcc
     2821 ggcgcccatc cgggaaccgg cgtggtgttg ttactcctct tccctgtcgc cttgcgggcg
     2881 ggaaggtcgg cgatcacgat tctcgccccc ggtttctccg gggtgccgtc atcgttgccg
     2941 cgggcctgcc acatgagcag cctggttccg tccgggctcc actgccccag ggtgcggttg
     3001 tcccagccgg tacccgggcg gggatcgacc ggctggccga agtaagtgcc gcgttcgcca
     3061 ccggaatcga gcagccacgg ctcgagcagg cacttccggt tcacctgatt cagctcgtag
     3121 cgcccgaccc aggaaaagag cgcgaattcg atgaaaggcg gccgcggcat caacgcgaag
     3181 atcggcatgc ggtcaaaccc gcgagagctg aactggaggt atgagccccc gtcgggggag
     3241 gtggaaacgt cctcttccca gtcgatgccc ctggtgatcc gggtccgttt gccgttccgg
     3301 aggtcaactc gccaggtatc gaagttgctt gcgtcatagg tggtggagta gaggaccgat
     3361 ttgccgtccg aggtgaagtt cttgagttcg aagtagggag actgggcgcg ccaggccgcc
     3421 gagtccgtcc ccagcggccc ggaaggattg aggatcctga cgttctcgag gtcgtactga
     3481 cccccgttct ttgccagacg cccgaccgac atcgccgtgc cgttcatcgt gaactcggtc
     3541 cagccgaacc agcggccatc gggcgaaagg cgcccctcgc ggttctgggt gccctggtct
     3601 attccgccgc caggcatgat gatcggggaa agcgtccgct cttcgcagtt gacgagcgtc
     3661 ggagtgcact ccacgactcc gtaggtgaaa ttctgggttg ggctgccccc ggcgccaggc
     3721 ctcaaaccga gccggaccat cagccgcttg ccgtcgggga acggtgccgg cttgccgaag
     3781 gccgcatcca ccggggcacc gggaacgacg gcggtgtcga ggccgcaggt gacgcacttc
     3841 ggatgggaac cgtcgaggcg ggtgatcgcc agctggggag cggtgaggtc gggtgacgcg
     3901 acgaccgtca cgaggcgctt tccgtcgggc atccagtccg gttccgactc gcccgagtac
     3961 cccgcgggaa cgggaaggtg gtgcacggag atcggctcaa ggggtgcggg cgccgcgtct
     4021 gcagcctgcg tgcaagcgaa caacgacagg agaaccaaca gccctgctgc tcctgcggac
     4081 accatcgaac cgatgcgctt catgtggccc ctcctcaagg gtcatttccc atgacggaaa
     4141 ccccgccgac acgatcatag cgtacgcatg cgtacttttt cagttggctg gaagggcctg
     4201 cttcgggaca ccgtttgccg gggttgaggc caatccggga aaaatcgcaa tgcgatacca
     4261 aatttcgggt actctgtttg ccggagttgt ttctccccag gcattcgagg gagtctcttg
     4321 ccgcagtacg gacgtcgttt cgatcacccg acgatgtcgg gaaaccggtc cacaaacccg
     4381 gaccgtgggc gccgctcggc cgttctgacc ggactgcttg ccgttctggc gctcctcctg
     4441 tgcttctctg cgacggctcc ggccgccggt gccagcaccg tttcaggcac cgtgagcgcc
     4501 gggggatcgc cggtgtccgg ggcggtcgtc gaagcgctcg acgtttcaac ttcggccgta
     4561 ctggccagcg acacgaccgg cgccgggggc agcttttcga tgactgtttc ggcctcttcg
     4621 gttgacgttc gggtcacgcc gccctcgggc tccggactcc agatatccac gatccacgcg
     4681 gtcgatacga ccagcaatgc gaacctggaa atcatcctcg ttcccaccgc atcccaggtc
     4741 cagctgtcag gaactctgcg tgacgaacac gggaacccgg ttgccaacgg gatcgttcaa
     4801 ctctcgagcg ggccctatgt ctacacggac gctgcgggcc actactcgtt cacgatgatg
     4861 cctccaggca cctacgccct gtacgtctac ggcgacaacc tgcccggact cccggcgcac
     4921 tggtccttct acaagccggg cgtggacatg agcgccgacc gggtacttga cctgaccctg
     4981 ccttcgaccg tctcgctttc cttcaaggtt caggacccgg acgggaatcc catttcgggc
     5041 gcccaggtgg tcggagtttc tgccagcggc aacgcgatga gcctgggacc gggcgtgacc
     5101 ggcgtgcagg gcactacata tatgaacggc gagtccgtga ccaccgatgc gaacgggatc
     5161 gcgcattaca cggtcttcgg gccggtcgat tcggtcagcg gggccgcggt cccgaaccag
     5221 cccggcttca gcaatgccgg gttcagcgtg tatccgttcg accagaacac gaccaccgtg
     5281 atcaccgtcc cgcggaccgt gcagctctcg ggaaccctgc gcgacgagca cggaaatccg
     5341 gtcgagaacg cgatcgtgca gctggcgggc ggcgcctacg tttacaccga cgcttccggc
     5401 cactactcgt tcacgacgac tccgggcacc ttcgccctgt acgtctatgg cgatgacctg
     5461 cccggcctgc cggcgcactg gtccttctac aagcccggag tggtcgtgaa cagcgaccgg
     5521 gtgctggatc tgaccctgcc ctcgacggtc tcgctttcct tcaaggtcca ggatcagaac
     5581 gggactccgg tcgcgggtgc gcaactggtg ggcgtttcgg cgagcgggaa ccacatgagc
     5641 cttggtcccg acgcacccga tgtgcagggc actacatata tgaacggcga gtccgtgacc
     5701 accgatggca acgggatcgc gcattacacg gtcttcgggc cggtcgattc ggtcagcggg
     5761 gccg
//
LOCUS       DAOQKN010000159         5741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_7342_length_5741_cov_5.588463, whole genome shotgun sequence.
ACCESSION   DAOQKN010000159 DAOQKN010000000
VERSION     DAOQKN010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5741
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7342_length_5741_cov_5.588463"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="PLJ64_09895"
     CDS             complement(<1..422)
                     /locus_tag="PLJ64_09895"
                     /inference="COORDINATES: protein motif:HMM:NF026066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNC93836.1"
                     /translation="MTNARIAEALEELAVLYQLDGADQYRVLAYTNSARTIKNEGRTV
                     EEMARNGTITELPGIGKTLEEKIVALVETGEIPAAAKLKERIPVGLLEISRIPGLGPK
                     TVRRLHDELDVNGPEDLRAAAEAQKVRELKGLGPKVEE"
     gene            466..1203
                     /locus_tag="PLJ64_09900"
     CDS             466..1203
                     /locus_tag="PLJ64_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HNC93837.1"
                     /translation="MPDEARTEHRGAAWVILPTYNEAGNVEKMVDALGPKLTHDDDRI
                     LIVDDNSPDGTGQIADRIATENPRVRVLHRKVKEGLGPAYIEGYRVALEGGADLIVQM
                     DADFSHDPAYLPRLLAASELADLVIGSRYVPGGGITEWGQVRRLLSRGGSLYSRTVLR
                     VPVRDMTGGFKCFRRKVLETIDLSQVAASGYSFQVEMTWRVVQAGFSVMEVPITFRER
                     QEGDSKMTTAIVAEAAWRVPAMRIRRH"
     gene            1343..1669
                     /gene="trxA"
                     /locus_tag="PLJ64_09905"
                     /pseudo
     CDS             1343..1669
                     /gene="trxA"
                     /locus_tag="PLJ64_09905"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
     gene            1690..1866
                     /locus_tag="PLJ64_09910"
     CDS             1690..1866
                     /locus_tag="PLJ64_09910"
                     /inference="COORDINATES: protein motif:HMM:NF013522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HNC93838.1"
                     /translation="MPFVQIHWFEGRSDEQKAEIAKRIEEALIEVAGAAPEHCWVKFE
                     DSKKTDFIIPEARS"
     gene            2024..2395
                     /locus_tag="PLJ64_09915"
     CDS             2024..2395
                     /locus_tag="PLJ64_09915"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNC93839.1"
                     /translation="MGVLNTDLGRQLEAKELGARLWRIEPGQASTRHRHKTTEELYLL
                     LEGTGKLRIGEELLTLEPMDAVVVEPETVRQPFNDTDADQLWLIVGAPTEHADTTSMD
                     EELIAWMYPDGPKAMPPELDP"
     gene            2423..3601
                     /locus_tag="PLJ64_09920"
     CDS             2423..3601
                     /locus_tag="PLJ64_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNC93840.1"
                     /translation="MERPESAAQVVGKRTIFEAEHDDYRESFRRFLEKEVVPEYPQWE
                     RDHIVPKSLFTKCAEYGFLSMEVPEEYGGPGVDDWRFNVVLAEESVWAGVGAGMGGPL
                     LHTDIVVPYIMSTANEEQKQRWLPQIASGEKVLAVAMTEPGTGSDLAAISTRGKRDGE
                     GWIVNGAKTFITNGINADQVVVAVRTSDDPHGGLSLFVIEEGMEGFERGKQIEKLGQH
                     ASDTSELFFNDCYVPQENMLGEEGSGFLQLVSRLVPERLTLSVSSMAGAEAALAMTID
                     YAKERKAFGRPIANFQHNRFTLAELRTEIEIGRCFIDRCIERYVDGTCTVEEAAMAKW
                     WTTELVSKVTDAGVQLHGGYGYTTEYRIGKAWVDARVGRIYAGTNEIMKELIGKTMGL
                     "
     gene            complement(3603..4202)
                     /locus_tag="PLJ64_09925"
     CDS             complement(3603..4202)
                     /locus_tag="PLJ64_09925"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HNC93841.1"
                     /translation="MASARPRAKTQAERRAETRAALLDATVESLVTYGYTGTTTGRIA
                     ELAGVSRGAQTPYFRSRADLLSAAVTHLAELRLAAVRERFSGKAITVEEGLDALWEEH
                     QGDAFDALLELWIASRTDDEMREHLVRIERDVGVAIVQESARALGPIADRPDFNDDMI
                     YILATMRGLSMMRISHGVSDRTLNRRWSEIRERLAQVLT"
     gene            complement(4240..>5741)
                     /locus_tag="PLJ64_09930"
     CDS             complement(4240..>5741)
                     /locus_tag="PLJ64_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HNC93842.1"
                     /translation="ICEAIDQTRGWFYTLLAESTLLFDQPSYRNCVCLGLILDGDGQK
                     MSKSKGNVVSPWEVLDEFGADAFRWYLFTAQQPWAGYRFSTEAISEALRSFLLTLWNT
                     YSFWVLYANAEGIDASTAPDPPAEPTDLDRWALSRLQATVGEVNAKMESFDCTAAGRA
                     IAAYVEELSNWYVRLSRRRFWEGDAAAFATLRHCLVEVSALLAPFTPFIAEEIHQNLA
                     GGADGDFGSLPDSVHLRDFPSPAAGLRDQELESAMAATRQTVELGRASRAQAKAKVRQ
                     PLARAVVVATDAERDAISTQTDLIKSELNVKELEFVTDQSDLVSYLVKPNFRTLGPRF
                     GKSMPQVSAAVAGLDPAHVAETVESGGEIGISIEGKDHTLTPEDLLLSMEPLEGYQVE
                     AESGHAVALALELDDELIREGLAREIVHAVQNARKDAGLEITDRIGLSLAGDQELLDA
                     AATHEGYVTGEVLARTIELGGDTAEAASPGTPEVTIDGRALKISVTRVD"
BASE COUNT          994 a         1836 c         1923 g          988 t
ORIGIN      
        1 ttctcttcga ccttcggtcc cagccccttg agttcacgga ccttctgcgc ctcggcggcg
       61 gcgcgcaggt cttcgggccc gttgacatcg agttcgtcat gcaggcggcg gacggtcttc
      121 gggccgagcc cggggatcct gctgatctcg agcaggccca ccgggatcct ctccttcagc
      181 ttcgccgcgg ccgggatctc gccggtttcg accagggcga cgatcttctc ttcaagcgtc
      241 ttgccgatcc cgggcagttc ggtgatcgtg ccgttccggg ccatctcctc gaccgtacgg
      301 ccctcgttct tgatcgtccg ggccgagttc gtgtaggcga ggacccggta ctgatcggcc
      361 ccgtccagct ggtagagaac ggccagctct tcaagcgctt cggcgatccg ggcgttggtc
      421 atacccagaa actacctcca accctttgag gggtacgatc cgccggtgcc ggatgaagcc
      481 aggacagagc accgcggcgc ggcctgggtc atcctgccga cctacaacga ggccggcaac
      541 gtcgagaaga tggtcgatgc actcggtccg aagctgaccc atgacgacga ccggatcctg
      601 atcgtcgacg acaattcccc ggacggtacc gggcagatcg ccgatcggat cgcgaccgaa
      661 aatccccggg tccgggtgct tcaccgcaag gtcaaggaag gtctcggacc cgcttacatc
      721 gaggggtacc gggttgcgct cgagggcggg gccgacctga tcgtccagat ggacgccgac
      781 ttctcccacg acccggccta cctgccgcgg cttctggccg cctcggagct tgccgacctg
      841 gtgatcggct cgcgctacgt gcccggcggc gggatcaccg aatggggcca ggtccggcga
      901 ctgctcagcc gtggcggcag cctttactcg aggacggttc tgcgggttcc ggtccgggac
      961 atgacgggtg gcttcaagtg cttccggcgg aaagtcctcg aaacgatcga cctctcccag
     1021 gttgccgcct ccggttattc cttccaggtc gaaatgacct ggcgggtcgt ccaagccggt
     1081 ttctcggtga tggaggtacc gatcaccttc cgcgaaaggc aggaaggcga ttccaagatg
     1141 acaactgcca tagtcgccga ggcggcatgg cgagtcccgg ccatgcgaat ccggaggcat
     1201 tgacccccgg agccggccca tctaaccttg agccctgtgg gccggcgggt tcgaccggga
     1261 ggggggtcgg accccgcccg actagttttc ccccatactt cattttgtat agtactagta
     1321 ccagttcaac gatcaggaga ccgacatggc acttcccgaa gtaaccgacg cgacgttcaa
     1381 ggccgaagca cttgaagccg atggcccggt gctggtcgac ttctgggccc cttggtgcgg
     1441 tccctgccgt gtggtccacc cggtgctcga agagatcaac gaagagcgcg acgacctcaa
     1501 gatcatcagc ctcaacatcg atgacaaccc gcagaccgcc cagcagtacg gggttctctc
     1561 gatcccgacg atgatcctgc tcaaggacgg cgccctggtc aagcagctgg tcggcgccca
     1621 gcccaagcgc aagattctcg ccgagctcga gccggccctt cagggctagt cggaccggga
     1681 cggatcaccg tgccgttcgt gcagatccac tggttcgagg gccgctccga tgagcagaag
     1741 gccgagatcg ccaagcgcat cgaagaggcg ctgatcgagg tcgccggggc agctcccgag
     1801 cattgctggg tcaagttcga agactcgaag aagaccgact tcatcattcc cgaggcgcgt
     1861 tcctgagtct gggggtggac accgtggggg ttcccttggt gtgcacagtc ccagtcggga
     1921 acccccacgg cgcccacccc tccgaacctg gaggaagacc ggtcccgtga gtgggtttcg
     1981 tcggttgagg gtgggggagc agttctggcg gcgttctaac cagatgggag ttctgaacac
     2041 cgatctgggt cggcagcttg aggcgaagga actcggggcc aggctctggc ggatcgaacc
     2101 gggccaggcc tcgacccggc accggcacaa gacgaccgag gagctttacc ttttgctcga
     2161 aggcaccggc aagctgcgga tcggggagga gctcctgaca ctcgaaccga tggacgcggt
     2221 tgtagtcgag ccggagacgg tgcgccagcc gttcaacgac accgatgccg accagctctg
     2281 gctgatcgtc ggcgctccga ccgagcatgc cgacaccaca tcgatggacg aggagctgat
     2341 cgcctggatg tacccggacg ggccgaaggc gatgccgccc gagcttgacc cctgatctgc
     2401 gcaagcggac taggcttccg gcatggagag accggagagt gcagcgcagg tagtcggcaa
     2461 gcggaccatt ttcgaagcgg agcacgacga ctatcgcgag agctttcgtc gtttcctcga
     2521 gaaggaagtg gtgcccgagt acccgcagtg ggagcgggat cacatcgttc ccaagagcct
     2581 gttcaccaag tgcgccgagt acggcttcct ctcgatggag gtgcccgagg agtacggcgg
     2641 ccccggcgtc gatgactggc gcttcaacgt ggtgctggcc gaggaatccg tctgggccgg
     2701 ggttggcgcg ggcatgggtg gccccctgct ccacaccgac atcgtcgtgc cctacatcat
     2761 gagcacggcc aacgaggagc agaagcagcg atggctgccg caaatcgctt ccggcgagaa
     2821 ggtgctggcc gtcgcgatga ccgaacccgg caccgggtcc gacctggccg cgatctccac
     2881 ccggggcaag cgggacggcg aaggctggat cgtcaatggt gccaagacct tcatcaccaa
     2941 cggcatcaac gccgatcagg tcgtggtcgc ggtccggacc tccgatgacc ctcacggggg
     3001 tctgtcgctt ttcgtgatcg aagagggcat ggaggggttc gaacgtggca agcagatcga
     3061 gaagctcggc cagcacgcct cggacacgtc cgagctcttt ttcaacgact gctacgtgcc
     3121 gcaggaaaac atgctcggcg aggaaggctc cggcttcctg cagctggtct cccggctggt
     3181 gccggaacgg ctgacactct cggtttcatc gatggccggc gccgaggctg ccctcgccat
     3241 gaccatcgat tacgcgaagg agcgcaaggc tttcggccgg ccgatcgcca acttccagca
     3301 caaccgcttc accctggccg aactgcggac cgagatcgag atcggccgct gcttcatcga
     3361 ccggtgcatc gagcggtacg tggacgggac ctgcacggtc gaggaggcgg cgatggccaa
     3421 gtggtggacc accgaactgg tcagcaaggt gacggacgcc ggagtccagc tccacggtgg
     3481 ttacgggtac acgaccgagt accgcatcgg caaggcctgg gtggacgccc gggtcggccg
     3541 gatctacgcc ggaaccaacg agatcatgaa ggaactgatc ggcaagacga tgggcctctg
     3601 actcaggtca gaacctgagc cagccgttcc cggatctcgc tccagcgtcg gttcagggtc
     3661 cggtcgctta caccgtggct gatcctcatc atggacaggc cgcgcatggt ggcgaggatg
     3721 tagatcatgt cgtcgttgaa gtcgggccgg tcggcgatcg gaccgagggc cctcgcggac
     3781 tcctgaacga ttgccacccc gacgtccctt tcgatcctga ccaggtgctc gcgcatttca
     3841 tcgtcggtcc gtgaagcgat ccacagttca agcagggcgt cgaacgcgtc accctgatgc
     3901 tcctcccaga gcgcgtcgag gccttcctcg acggtgatcg ccttgccgct gaaccgttcc
     3961 cgcacggcgg ccagcctcaa ctcggcgaga tgggtcacgg cggcactgag caggtccgcc
     4021 cggctgcgga aatagggggt ctgtgccccg cgggaaacgc cggccagttc ggcgatccgg
     4081 ccggtggtgg tcccggtgta cccgtaagtg accagcgatt cgacggtcgc atccagcagc
     4141 gccgcgcgtg tctccgcgcg gcgttcagcc tgggttttcg cgcggggacg agcggaagcc
     4201 attgggtgaa gatatcggcc cggcgttggc cggcgattct cagtcgaccc gggtgaccga
     4261 gatcttcagc gcccggccgt cgatcgtcac ttcgggggtt ccagggctgg ccgcctcggc
     4321 cgtgtcgccg ccgagttcga tcgtccgggc cagaacttct ccggtcacgt agccctcatg
     4381 ggtggcggcg gcatcgagaa gttcctggtc gccggccagt gaaaggccga tccggtcggt
     4441 gatctcgagg ccggcatcct tgcgggcgtt ctggacggcg tggacgatct cgcgggccag
     4501 gccctcgcgg atcagctcgt cgtcgagctc gagggcgagg gcgactgcgt ggcccgactc
     4561 ggcttccacc tggtagccct cgagcggttc catgctgagc agcaggtcct ccggggtgag
     4621 cgtgtggtcc tttccttcga tcgagatccc gatctcgccg cccgactcga ccgtctcggc
     4681 cacgtgggcc ggatcgagcc cggcgaccgc agcggaaacc tgcggcatcg acttgccgaa
     4741 gcgcgggccg agggtgcgga agttcggctt gaccagatag ctgaccagat ccgactggtc
     4801 ggtcacgaac tccagttcct tcacgttcag ttcgctcttg atcagatcgg tctgggtcga
     4861 gatcgcgtcc cgctcggcgt cggtcgcaac gacgaccgcc cgggccagcg gctggcggac
     4921 cttcgccttg gcctgggccc gcgaggcccg gccgagctcg accgtctgtc gggtagccgc
     4981 catcgccgac tcgagttcct ggtcccggag tcctgccgcc ggcgagggga agtcgcgcag
     5041 gtggaccgag tcgggcagcg acccgaaatc gccatcggcc ccgccggcca ggttctggtg
     5101 aatctcctcg gcgatgaacg gggtgaaggg ggcgagcagg gccgacacct cgaccaggca
     5161 atgcctcagg gttgcgaagg cggcggcatc gccctcccag aagcggcgac gggaaagccg
     5221 cacataccag ttggagagtt cctcgacgta ggccgcgatc gcccggccgg cggcggtgca
     5281 gtcgaagctt tccatcttcg cgttgacttc cccgaccgtg gcctgcaggc gggaaagtgc
     5341 ccagcggtcg aggtcggtcg gctcggctgg cgggtccggc gcggtcgatg cgtcgatgcc
     5401 ctccgcgttc gcgtagagaa cccagaagga gtaggtgttc cagagggtca agagaaagga
     5461 acgaagcgct tccgagatcg cctcggtcga gaagcggtag ccagcccagg gctgctgggc
     5521 ggtgaacagg taccagcgga aggcatcggc gccgaactcg tccagcacct cccaggggga
     5581 aaccacattg cccttcgact tcgacatctt ctggccgtca ccgtcgagga tcaggccgag
     5641 gcagacgcag ttccggtagc tgggctggtc gaacagcagg gtcgactcgg ccagcagcgt
     5701 gtagaaccag ccgcgggtct ggtcgatcgc ctcgcagatg t
//
LOCUS       DAOQKN010000160         5737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_7352_length_5737_cov_6.090813, whole genome shotgun sequence.
ACCESSION   DAOQKN010000160 DAOQKN010000000
VERSION     DAOQKN010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5737
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7352_length_5737_cov_6.090813"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            255..1472
                     /locus_tag="PLJ64_09935"
     CDS             255..1472
                     /locus_tag="PLJ64_09935"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNC93843.1"
                     /translation="MNQTSDAPKRSLRSVFLAALTGGLVVAVAGMIAIAAGWIGKTET
                     TVQSLTATTTNASSKNDQNLVNQIYNRDSDGVGFITASGISTGSTNPFDPFGQQQGTS
                     TATGSGFLIDTDGHMVTNNHVIEGAKKITVTLGDSDAVYDASVVGTDPSTDLAVIKVD
                     APESEMKPLALGDSTDVKVGDPVVAIGNPFGLDRTVTTGIVSALQREISSLTNYAISN
                     VIQTDAAINPGNSGGPLIDANGNVIGVNSQIATGSGSSTSGNVGIGFAIPSNTVKDVV
                     GQLIEDGKAEHAYLGISGATINEQLANALNLGTDTGVLVQEAPKDGPAAQAGIKAGDT
                     AVTIQGQRVMAGGDVITKVNGTDLGSMEDLIDAVNGAKVGDEMELEVQRDGQTSTVTV
                     KLGTRPDDTQPSK"
     gene            1506..2144
                     /locus_tag="PLJ64_09940"
     CDS             1506..2144
                     /locus_tag="PLJ64_09940"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF002298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HNC93844.1"
                     /translation="MKVKVCGITNRDDAEAAVEHGAWAIGLIHHKESPRFVKASVAAG
                     IGEEFRRRCEVVGVFVNPTIERVVQAAENAQLTMIQLSGNEGANFCSEVNRRTGLKVI
                     KAFHISSQADVKASTAYRFTDFHLFDTGRKGVYGGTGETFDWDLLVSYHSPIPTILAG
                     GLNPDNVTEAIRIVRPDAVDVASGVEREPGIKDHGKLASFFQAARYTPVAGA"
     gene            2141..3349
                     /gene="trpB"
                     /locus_tag="PLJ64_09945"
     CDS             2141..3349
                     /gene="trpB"
                     /locus_tag="PLJ64_09945"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HNC93845.1"
                     /translation="MSGHFAESTAGELPGRFGPYGGRYVPETLIPALDELTVAWAEAR
                     KDPAFTGELNVLLRDYVGRPSPLYLASRLSEEAGRKVYLKREDLNHTGAHKINNAIGQ
                     ALLARRMGKPRIIAETGAGQHGVASATACALLDLECVVYMGSEDIRRQKPNVERMKLL
                     GAEVVPVEAGARTLKEAVSAAIRDWVANVADTHYIIGSAVGPAPFPELVRDLQRVIGD
                     EARDQALQAEGELPGRVIACVGGGSNAIGTFTAFIPDEEIELIGVEAAGHGLDTDKHG
                     ASLTAGNTSVLHGSLSAVLTDEEGQILEAHSVSAGLDYPGTGPEHAHLRDIGRARYVA
                     VDDRDAIAAFRRLSRLEGIIPALESSHAIAWLLGEGKIGDADGNYDVLCLSGRGDKDL
                     AEVLGLEEAG"
     gene            3346..4164
                     /gene="trpA"
                     /locus_tag="PLJ64_09950"
     CDS             3346..4164
                     /gene="trpA"
                     /locus_tag="PLJ64_09950"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HNC93846.1"
                     /translation="MTGAERIAAAFAAAGEEGRAALMPYMMGGFPDRETSTAIARSYA
                     ENGADLIELGVPFSDPLADGPVIHEAAVKALEAGTKLDDVLAICREVADRVPVVLMVY
                     TNTILGGAGPEAFAAKAAEAGACGVIVPDLPLGEDEKIRETMTAAGLAVVPLVAPTTL
                     GERRAEICRIASGFVYVVSSVGTTGERAEVPAHLKELVEDVESKSKVPVAVGFGIGSP
                     EHAAIVGQTADGVIIGSKLVRLAAEAAPGQAAEAVGEFMSEAAAAVASARSGVR"
     gene            4176..4355
                     /locus_tag="PLJ64_09955"
     CDS             4176..4355
                     /locus_tag="PLJ64_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93847.1"
                     /translation="MLIPVTFFVALALAVFSYSQGQGGPVAGLVFFGVLFIGALIHVC
                     QPLIEKGKALRPSKD"
     gene            4360..5385
                     /locus_tag="PLJ64_09960"
     CDS             4360..5385
                     /locus_tag="PLJ64_09960"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNC93848.1"
                     /translation="MKIGVLTGGGDCPGLNAVIRAVVLRGTREWGHEFTGYLDGWKGP
                     LENESIELGVGDVRGILAQGGTILGSSRTNPEAIEGGVDQVIDNVRAAGLDGLIAIGG
                     DDTLGVARILTESGLGVVGVPKTIDNDLNATDYTFGFDTAVNIVMESLDRLHTTATSH
                     HRCLVVEVMGRHAGWIALHGGMAGGADQILVPEEEFDLEQVCRQVNRSFEAGVAPIVV
                     ASEGAIPAEGSELTTAEGTDAFGHVRLGGIGGWLAERIEENTGHETRSVVLGHLQRGG
                     TPTAFDRVLATRLGSRAIQAANEGEWGTMTTLRGTSIELAPLADAVDESKVVPPERLR
                     EAQALFD"
     gene            complement(5440..>5737)
                     /locus_tag="PLJ64_09965"
     CDS             complement(5440..>5737)
                     /locus_tag="PLJ64_09965"
                     /inference="COORDINATES: protein motif:HMM:NF019938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1731 domain-containing protein"
                     /protein_id="HNC93849.1"
                     /translation="HDEIGILLWALENESVSGPVNATAPNPVTNKEFSKALGKQLGRP
                     SFLPVPGFAIDIVKGGGVGHAAREGQRVFPKKATDGGYEFKQPELAGALANLLD"
BASE COUNT         1017 a         1890 c         1927 g          903 t
ORIGIN      
        1 tcgggtgggg gtcgtccggg acactccagg accagcgcgg ctgaatgccg cgcgtgcgtc
       61 cgtcccggct acccccaccc gaccgcacgg cgtaacctcc tggccccgcg gcgtggccgg
      121 acggccccgc ggcgtggccg gacggccccg tggcacggtc ggaagggccg ggattcccag
      181 ccggttccca ggtttaggaa accttagaat ttggcttagg aatggtcaat gagggtggtc
      241 tttatttccc cgtcatgaac cagacttccg atgccccaaa gcgctctctg cgctccgtgt
      301 tcctggccgc cctcaccggc ggcctcgttg tcgccgtagc cggcatgatc gcgatcgccg
      361 ccggctggat cggcaaaacc gagacgaccg tgcagtcgct gaccgcgacc accaccaacg
      421 cctcctcgaa gaatgaccag aacctggtca accagatcta caaccgcgac agcgacgggg
      481 tgggcttcat caccgcctcg ggcatctcca ccggttcgac caatcccttc gatccgttcg
      541 gccagcagca gggcaccagc accgcgaccg gttccggttt cctgatcgac accgacggtc
      601 acatggtcac caacaaccac gtgatcgagg gcgcaaagaa gatcaccgtc acgctcggtg
      661 attcggacgc ggtctatgac gcgagcgtgg tcggcaccga cccctcgacc gacctggccg
      721 tgatcaaggt cgacgcgccg gaaagcgaaa tgaagccgct cgcgctcggt gactcaaccg
      781 acgtaaaggt cggtgacccg gtcgtcgcga tcggcaaccc gttcggcctc gaccgcacgg
      841 tcaccaccgg catcgtctcc gcccttcagc gggagatatc gagcctcacc aactacgcga
      901 tttcgaacgt gatccagacc gacgccgcga tcaaccccgg caactccggc ggaccgctga
      961 tcgacgccaa cggcaacgtg atcggagtca actcgcagat cgccaccggt tccggctcca
     1021 gcaccagcgg caacgtcggc atcggtttcg cgattccctc gaacaccgtc aaggacgttg
     1081 tcggccagct gatcgaggac ggcaaggccg aacacgccta cctcggaatc agcggcgcga
     1141 cgatcaacga gcagcttgcc aacgcgctca acctcggaac cgacaccggc gtcctggttc
     1201 aggaagcgcc gaaggacggc cccgctgccc aggccggaat caaggccggt gacaccgcgg
     1261 tcacgatcca gggccagcgg gtgatggccg gaggcgacgt gatcaccaag gtcaacggca
     1321 ccgatctcgg ttcgatggaa gatctgatcg atgccgtcaa cggggcaaag gtcggcgacg
     1381 agatggaact cgaagtccaa cgcgatggtc agaccagcac ggttacggtc aagctcggca
     1441 cccggcccga tgacacccag ccgtcgaaat gacggccgga cgggcggttg tcataccctt
     1501 gactggtgaa ggtaaaggtc tgtggaataa ccaatcggga tgacgccgaa gccgccgttg
     1561 aacacggcgc ctgggcgatc gggctgatcc atcacaagga gagcccccgg ttcgtcaagg
     1621 cgtcggttgc ggccggcatc ggcgaggagt tccgtcgccg ctgcgaagtg gtcggcgtct
     1681 tcgtcaaccc gaccatcgaa agggtggtcc aggcggccga gaacgcgcag ctgacgatga
     1741 tccagctgag cggcaacgag ggcgcgaact tctgcagtga agtgaaccgt cgcaccggcc
     1801 tgaaagtgat caaggctttt cacatctcca gccaggcgga tgtcaaggcc agcaccgcct
     1861 accgcttcac cgatttccac cttttcgaca ccggccgcaa aggcgtttac ggcggcacgg
     1921 gagagacttt cgactgggac ctcctggtca gctaccactc gccgatcccg accattctgg
     1981 ccggggggct gaaccccgac aacgtgaccg aggcgatccg gatcgtccgg cccgacgcgg
     2041 tggacgttgc ttcaggggtc gagcgcgagc cggggatcaa ggaccacggc aagctggcca
     2101 gcttcttcca ggcagcccga tacaccccgg tggcaggcgc atgagcggcc acttcgcgga
     2161 gtccacggcc ggcgagctgc cgggccgctt cggcccctac ggcggccgtt acgttcccga
     2221 gaccctgatc cccgcgcttg acgagctgac cgtcgcctgg gcggaggccc gaaaggaccc
     2281 ggctttcacc ggggagctga acgtcctgtt gcgcgactac gtcggccggc cctcgccgct
     2341 ctatctcgct tcccggctga gcgaggaggc gggccgcaag gtctacctga agcgggagga
     2401 cctcaaccac accggcgccc acaagatcaa caacgcgatc ggccaggcgc ttctggcgag
     2461 gcggatgggc aagccgcgga tcatcgccga gaccggggcc gggcagcacg gcgtcgcgag
     2521 tgccacggcc tgcgccctgc tcgacctcga gtgcgtcgtc tacatgggca gcgaggacat
     2581 tcgccgccag aagccgaacg tcgaacggat gaagctgctc ggggccgaag tggttccggt
     2641 cgaggccggc gcccgcaccc tgaaggaggc ggtcagcgcc gcgattcgcg actgggtggc
     2701 aaacgtcgcc gacacccact acatcatcgg ctccgcggtc ggtccggctc cttttccgga
     2761 actggtccgt gacctccagc gggtgatcgg ggacgaagcc cgcgaccagg ccctgcaggc
     2821 cgagggcgag ctgcccggcc gcgtgatcgc ctgcgttgga ggcggttcca acgcgatcgg
     2881 caccttcacg gcattcattc ccgacgagga aatcgagctg atcggcgtcg aagctgccgg
     2941 tcacgggctc gataccgaca agcacggagc ttcgctcacc gccggcaaca cctcggtcct
     3001 ccacggctcg ctttcggccg tgctgaccga cgaggaaggg cagatcctcg aagcccattc
     3061 ggtttcggcc gggctcgact accccggcac cggccccgag cacgcccatc tcagggacat
     3121 cggccgggcc cggtacgtgg cggtcgacga ccgggacgcg atcgcggcct tccggcgcct
     3181 gagccggctc gaagggatca ttccggcgct cgagtcgtcg catgcgatcg cctggctgct
     3241 cggcgaaggg aagatcggtg acgccgacgg caattacgac gtgctttgcc tgtccggccg
     3301 cggcgacaag gacctggccg aggtgctggg cctggaggag gccggatgac gggcgcggag
     3361 cggatcgccg cggccttcgc ggctgccgga gaggaaggtc gcgccgcgct gatgccgtac
     3421 atgatgggcg gctttcccga ccgggagact tcaaccgcga tcgcgcgctc ctatgccgaa
     3481 aacggggccg acctgatcga actcggcgtg cccttctcgg accccctggc tgatgggccg
     3541 gtgatccacg aggccgcggt gaaggcgctt gaagcgggta cgaagctcga cgacgtgctg
     3601 gcgatctgcc gcgaggtggc cgaccgggtg ccggtggtgc tgatggtcta cacgaacacg
     3661 atcctcggtg gagcgggtcc ggaagccttc gctgcgaagg cagccgaagc cggcgcctgc
     3721 ggggtgatcg tgcccgacct gcccctcggc gaagacgaga agatccgcga gacgatgacc
     3781 gccgccgggc tggctgtggt tccgctcgtg gcgccgacga ccctgggcga gagaagagcc
     3841 gagatctgcc ggatcgccag cggtttcgtc tatgtggtct ccagtgtcgg cacgaccggc
     3901 gaacgagccg aggtgcccgc tcacctgaag gagctggtcg aagatgtgga gtcgaagtcg
     3961 aaggttccgg tggcggtcgg attcggcatc ggatcacccg aacatgcggc gatcgtgggc
     4021 cagacggccg acggagtgat catcggttcg aaactggtcc ggctggccgc cgaggcggca
     4081 ccgggacagg cagcagaggc ggtcggtgaa ttcatgtccg aagctgccgc ggccgtggca
     4141 tcagcccgct ccggtgtcag gtaggattcc cgccgatgct gatcccggtc acattcttcg
     4201 tcgctctcgc cctcgcggtg ttctcgtact cgcagggcca gggtggtccg gttgccggcc
     4261 tcgtcttctt cggtgtgctg ttcattggcg cgttgattca cgtctgccag ccgctcatcg
     4321 aaaagggcaa ggcgctccgg ccctcaaagg actaacccca tgaagatcgg ggtgttgacc
     4381 ggcgggggtg attgccccgg cctcaatgcc gtgatccgtg cggtggtgct cagaggaacc
     4441 cgtgaatggg gtcacgagtt caccggctat ctcgacggct ggaagggtcc gcttgaaaac
     4501 gagtcgatcg aactcggggt tggcgacgtc cgcggcatcc tcgcccaggg cggaaccatc
     4561 ctcggttcgt cccgaaccaa cccggaagcg atcgaaggcg gggtggacca ggtgatcgac
     4621 aatgtccggg cggccggcct cgacgggctg atcgcgatcg gcggcgacga caccctcggc
     4681 gtcgcccgga tcctcacgga gagcggactc ggagtggtcg gcgtgccgaa gacgatcgac
     4741 aacgatctga acgccaccga ttacaccttc ggtttcgaca ccgccgtcaa catcgtgatg
     4801 gagtccctgg accggctgca cacgaccgcc accagtcatc accgctgcct ggtggtcgag
     4861 gtgatgggtc gtcacgccgg ctggatcgcg cttcacggcg ggatggccgg tggcgccgac
     4921 cagattctcg tgccggaaga ggagttcgac ctcgagcagg tctgccgtca ggtcaaccgc
     4981 agcttcgagg ccggggttgc cccgatcgtc gtcgcttccg agggcgcgat tccggcggaa
     5041 ggcagcgagc tcaccacggc cgaggggacc gacgccttcg gtcacgtgcg gctcggcggc
     5101 atcggcggct ggctggcgga acggatcgag gagaacacgg ggcacgagac gaggtcggtc
     5161 gtgctcggcc atctccagcg gggtggaacc ccgaccgcct tcgaccgggt gctggccacg
     5221 cggcttggaa gccgggcgat ccaggcggcc aacgaaggcg aatggggcac catgaccacg
     5281 cttcggggta cctcgatcga actcgccccg ctggccgacg ccgtggacga atcgaaagtc
     5341 gtcccgccgg agcggcttcg cgaagcacag gctctcttcg actgaatccc gaatcatcct
     5401 gccctcggga aagggaagga agttcacaac ggggatcgct cagtcgagca ggttggcgag
     5461 ggcgcccgcc agttccggct gcttgaactc gtagccgccg tcggtcgcct tcttggggaa
     5521 gaccctctga ccctcacggg cggcatggcc gactccgccg cccttgacga tgtcaatcgc
     5581 gaagccgggc acgggcagga acgagggccg tcccagctgc ttgccgagag ctttcgagaa
     5641 ttccttgttg gttacggggt taggtgccgt cgcgttgacc gggccgctga ccgactcgtt
     5701 ttcaagggcc cagagcagga tcccgatctc gtcgtgc
//
LOCUS       DAOQKN010000161         5731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_7364_length_5731_cov_6.254581, whole genome shotgun sequence.
ACCESSION   DAOQKN010000161 DAOQKN010000000
VERSION     DAOQKN010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5731
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7364_length_5731_cov_6.254581"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="PLJ64_09970"
     CDS             <1..588
                     /locus_tag="PLJ64_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93850.1"
                     /translation="GDYSYGWYKQAFRRIAIITRGGAKRGFINARLKDLRLPKVQNVG
                     QSRRLPRRLPKAPVSLVWSPLPAGSPTVRANLPARYWPGKDWVDWVGTDFYNRYNNWK
                     HLGRFYKKWALGKNKPMALTEFGLWGSDSPSFIKRIFKFTRNRKKIRMMVYYQDFGES
                     NSFRIQNFPQGRKVLSQFLNKRDYPKFAPKYPRFR"
     gene            complement(655..1812)
                     /locus_tag="PLJ64_09975"
     CDS             complement(655..1812)
                     /locus_tag="PLJ64_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNC93851.1"
                     /translation="MNPRDYLEIDRLLTDEERLIKETVSRYVNEKYLPGVAEHFENGT
                     FDTAVPKALGDLNVLGMHLKGYGCAGTSAVAYGVACLELEAGDSALRSFCSVQGSLAM
                     FAIWKWGSEEQKQRWLPGMAAGDLIGCFGLTEPDSGSDPGSMRTTAKKDGEDWILNGS
                     KMWITSGSIADVAVVWAKTDDGVQGFLVEPGMKGFTAPEMHHKLSLRASVTSELAFDD
                     VRIPDANRLPEVQSLKGPLSCLNEARYGIVWGVVGAARSCFEAALDYAMTRKQFDEPI
                     ASFQLTQAKLADMATKVQQGYLLALRLGQLKDEGRIEPEHISMGKRANARMALDVART
                     ARGILGANGITLEYPVLRHANNLESVFTYEGTDEIHTLSIGMALTGIPAFR"
     gene            complement(1867..4698)
                     /locus_tag="PLJ64_09980"
     CDS             complement(1867..4698)
                     /locus_tag="PLJ64_09980"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNC93852.1"
                     /translation="MIFRKLAGIGIVSIVVAMLTAATGAGLAGAATITVNSTGDAVSD
                     AGQCVLREAINAANTDTASGVTPGECAAGSGADTVVFNFDPLPGPHTLVPATEMPVLT
                     SLVTIDATAEAHEVILDGSAAAAAPGSQIGFDIQADGASVKGLTIGNWDYGVYVFDAD
                     DVSISGNRIGIDLGDSARPNGVVGIQVEGDSDRTQVGSNVISGNAQQGLRMVGGQVGD
                     PLITGNRIGTNNAGDAAIPNGEGIWLSDTTGAVVGGDTVAEGNLISGHPSTAINLTGG
                     PTDYTSNVLIQGNTLGLNAAGDAALGNLSGVHIVGDVRDSVIEDNVISGSTQWGIDIG
                     PVFGVTETPSGLKIAGNRIGTNSAGTAAIPNTFEGIKVDAAGSPFGAGILIGGITGLT
                     PGGPCTGDCNLISGNLRHGVAIDDGSQFVSILGNSIVGNQLLGIDLRDLALSGGTPLV
                     NDADDVDGGSNGFQNHPVLQAALNQAGKLRVTGILNSVADRNYRIEIFGNGAADSSGY
                     GEGETFLGAFTVSNAPGQTSVGFAETLSVPVATGNTLVATATVLDPVTGRPASSEFSI
                     NSTEGCDQSGDPDPNELTASAGGEAICGQGGADVLKSGVAGDILRGDAGSDSADFSGL
                     PYAVSASLASGLARAIPLASGVADILFTVENLRGSPQDDTLTGDTDTNVILGAAGDDE
                     INPGGGPDSVYGGPDDDTINVVDGLADNLIDCGTGVDTVTADPTATEPASLFVDCETI
                     NRPGDPPPTCETDPDLCPPPTCETDESLCPPEPVVYKCDGIKATIVGTNHDETINGTN
                     QRDVIVARAGKDKINPKKGNDLICAGDGVDSVSGSGGNDRALGQDGADNLKGGDGNDK
                     LLGGAKDDKLYGHAHGDILKGGEGSDRLEGGANADTLYGEGHGKDRLFGNSGFDKLSG
                     GDGGKDQCDGGTNKDRRKAPGCEQRKRLP"
     gene            4828..5157
                     /locus_tag="PLJ64_09985"
     CDS             4828..5157
                     /locus_tag="PLJ64_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93853.1"
                     /translation="MQIGGTLINGLVRVLVVAGTLAAVYYFVVRPVLDTTEKVSGGIN
                     SSIQQTLDEANDAFDQTNVTTKNQKVLTRKIKNVPTSNIPKLENCITAAGTNINQINR
                     CINRYAN"
     regulatory      5223..5373
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            5379..5513
                     /locus_tag="PLJ64_09990"
     CDS             5379..5513
                     /locus_tag="PLJ64_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93854.1"
                     /translation="MDLDRHVVRPMTEETGARAAFGMLVGALAGTAIALPLLVGVFGL
                     "
BASE COUNT          940 a         1923 c         1834 g         1034 t
ORIGIN      
        1 ggcgactact catacggctg gtacaagcag gccttccgcc ggatcgcgat catcacccgc
       61 ggcggcgcca agcgcggttt catcaacgcc cggttgaagg accttcgact gcccaaggtt
      121 cagaacgtcg gccagtccag gcgccttccc cgccgcctgc cgaaggcccc cgtctcgctg
      181 gtctggagcc cgttgccggc cggctccccg accgtccggg ccaacctccc ggcccggtac
      241 tggccgggca aggactgggt cgactgggtc ggcaccgact tctacaaccg ctacaacaac
      301 tggaagcacc tcggccgctt ctacaaaaag tgggccctgg gcaagaacaa gccgatggcc
      361 ctgaccgagt tcgggctctg gggcagtgac agcccgtcct tcatcaagcg gatcttcaag
      421 ttcacccgca accggaagaa gatccggatg atggtctact accaggactt cggcgagtcg
      481 aactccttcc ggatccagaa cttcccgcag ggccgcaagg tcctgtcgca gttcctcaac
      541 aagcgcgact acccgaagtt cgccccgaag tacccccgct tccggtaggc ccccgagcgg
      601 ccaggagcca cggtattcca caaatactgt ggaaaaccgc tggtcccggg ttgatcagcg
      661 gaaggccggg atgccggtca gggccatgcc gatcgagagg gtgtggatct cgtcggtgcc
      721 ctcgtaggtg aagacagatt cgaggttgtt ggcgtggcgc aggaccgggt actcgagggt
      781 gatcccgttg gcaccgagga tgccacgggc ggtgcgggcc acatccaggg ccatccgggc
      841 attggcccgc ttgcccatcg aaatgtgctc cggctcgatc cggccctcgt ccttgagctg
      901 gccgagcctg agggcgagca ggtagccctg ctggaccttg gtcgccatgt cggcgagctt
      961 ggcctgggtg agctggaagg aagcgatcgg ctcgtcgaac tgcttgcggg tcatcgcgta
     1021 atcgagtgcc gcctcgaagc atgagcgggc agcgccaacg actccccaga cgattccgta
     1081 gcgggcttcg ttcaggcagc tgagcgggcc cttcagcgac tggacttcgg gcaggcggtt
     1141 ggcgtcgggg atccgcacgt cgtcgaaggc cagctccgaa gtgaccgagg cccgcagtga
     1201 aagcttgtgg tgcatttccg gggcggtgaa gcccttcatg cccggctcga ccaggaaacc
     1261 ctgaacgccg tcatcggtct tcgcccagac cacggccacg tcggcgatcg aaccggaggt
     1321 gatccacatc ttcgagccgt tgaggatcca gtcctcgccg tccttctttg cggtggtccg
     1381 catcgacccg gggtcggagc cggagtccgg ttcggtcagg ccgaagcagc cgatcagatc
     1441 accggccgcc atgcccggca gccagcgctg cttctgctcc tcgctgcccc acttccagat
     1501 cgcgaacatg gccagtgaac cctgcaccga gcagaaggac ctgagggccg aatcgccggc
     1561 ctccagctcc aggcaggcga cgccgtaggc gaccgcgctg gttccggcgc agccgtatcc
     1621 cttcaggtgc atgcccagca cgttgaggtc gccgagtgcc ttcggcacgg cggtgtcaaa
     1681 ggtcccgttc tcgaagtgct ccgccacccc gggcaggtac ttctcgttca cgtaacggga
     1741 aacggtctcc ttgatgaggc gctcctcgtc cgtgaggagc cggtcaattt ccagatagtc
     1801 gcgcgggttc acagaagaca atttaacgat ccggccttcg cccgaccgcg aaaccggccg
     1861 gtccgctcag ggcagccgtt tcctttgctc gcagcccggg gccttcctcc ggtccttgtt
     1921 cgtgcctccg tcgcattgat ccttgccacc gtctccgccg ctcagtttgt cgaagccgga
     1981 attgccgaag agcctgtcct tgccgtggcc ttcgccgtag agggtgtcgg cgttggcacc
     2041 gccttcgagg cggtccgacc cttccccgcc cttgaggatg tcaccgtggg cgtggccgta
     2101 gagcttgtcg tccttggcgc cgccgagcag tttgtcgttg ccgtcgccgc ccttgaggtt
     2161 gtcggccccg tcctggccca gggcccggtc gttgccgccg ctgccggaaa ccgagtcgac
     2221 cccgtcaccg gcgcagatca ggtcattgcc ctttttcggg ttgatcttgt ccttgccggc
     2281 ccgggccacg atcacgtccc gctggttggt gccgttgatc gtctcgtcat ggttggtgcc
     2341 gacgatcgtc gccttgattc cgtcgcattt gtagacgacc ggttccggcg gacaaaggct
     2401 ttcgtcggtc tcgcaggtcg gcggcgggca gaggtcgggg tcggtctcac aggtcggcgg
     2461 cgggtcgccc ggccggttga tcgtctcgca gtcgacgaac aggctggccg gttcggtcgc
     2521 ggtcgggtct gcggtcaccg tgtcaactcc ggttccgcaa tcgatcaggt tgtcggccag
     2581 cccgtcaacc acgttgatcg tgtcgtcgtc cgggcccccg taaacggagt cggggccgcc
     2641 gccggggttg atctcgtcgt ctcccgcggc gccgagaatc acgttcgtgt cggtgtctcc
     2701 ggtcagggtg tcatcctgcg ggctgccgcg caggttctcg accgtgaaca ggatgtcggc
     2761 aaccccggaa gcgagcggaa tggctctcgc caggccagat gccaggctgg ccgacaccgc
     2821 gtacggcagg cctgaaaagt cggccgagtc cgagcccgcg tcgccccgca ggatgtcccc
     2881 cgcgactccg ctcttcagca cgtcggcacc gccctgcccg cagattgctt ctccgccggc
     2941 cgaggccgtc agttcgttcg ggtcgggatc cccgctctgg tcgcaaccct cggttgaatt
     3001 gatcgagaac tcggaactgg caggccggcc ggtcaccggg tcgaggacgg ttgccgtggc
     3061 caccagagtg ttcccggtgg cgaccggcac cgacagcgtc tcggcgaacc ccacgctggt
     3121 ctggccgggc gcgttcgaca cggtgaaggc accgagaaag gtctcccctt cgccgtatcc
     3181 gctggagtcg gccgccccgt tgccgaagat ctcgatccgg tagttccggt cggcgacgga
     3241 gttgaggatg ccggtcaccc gcagcttccc ggcctggttc agggcggcct ggaggacggg
     3301 atggttctgg aaaccgttgc ttccgccatc aacgtcatcg gcatcgttga ccagcggcgt
     3361 acccccggaa agcgccaggt cccgaaggtc gatgccgagc agctggttgc cgacgatcga
     3421 gttccccagg atcgagacga actggctgcc gtcgtcaatc gccacgccgt gccggagatt
     3481 gcccgagatc aggttgcagt caccggtgca ggggcccccg ggtgtcaggc cggtgattcc
     3541 gccgatcaga atccccgccc cgaacggact gccggcagcg tcaaccttga tgccttcaaa
     3601 agtgttgggg atggcagcgg tcccggcgct gttcgtgccg atccggttgc cggcaatctt
     3661 cagcccggat ggcgtttcgg tcacgccgaa gacggggccg atgtcgatcc cccactgggt
     3721 gctgccggaa atcacgttgt cctcgatgac cgagtcccgg acatcgccga cgatgtgaac
     3781 gccggacagg ttccccaacg ccgcgtcacc tgccgcgttc agccccagcg tgtttccctg
     3841 gatcaggaca ttgctcgtgt agtcggtcgg cccgccggtc aggttgatcg ccgtgctcgg
     3901 gtggccggaa atcaggttcc cttccgcaac cgtgtcaccg ccgaccacgg cgcccgtggt
     3961 gtcgctcagc cagattcctt cgccgttcgg aatggcggcg tcgccggcgt tgttcgtgcc
     4021 gatccggttt ccggtgatca gcggatcgcc gacctggccg ccgaccatgc gaagcccctg
     4081 ctgcgcgttg cccgaaatca cattggaacc gacctgcgtg cggtccgaat ccccttcgac
     4141 ctgaatgccg acgacaccgt ttggcctggc ggagtcaccg aggtcgatgc cgatccggtt
     4201 gccggagatc gacacgtcgt ctgcatcgaa cacatagacc ccgtaatccc agttgccgat
     4261 cgtcaatccc ttgaccgagg cgccgtccgc ctggatatcg aaaccgatct gggatccggg
     4321 ggcggccgcc gcggccgagc cgtcgaggat cacttcatgg gcttccgccg tcgcgtcaat
     4381 ggtcaccagg ctcgtcagga cgggcatctc ggtggccgga acgagggtgt ggggtccggg
     4441 cagtggatca aagttgaaga caaccgtgtc ggcgccgctg ccggcggcac attcgccagg
     4501 tgtcacgccg ctcgccgtgt ccgtgttggc ggcgttgatc gcttccctca gaacgcactg
     4561 acccgcatcg gagacggcgt caccggtgct gttcacggtg atcgtggccg caccggcaag
     4621 tccggctccg gtggcggccg tcagcatcgc caccacgatc gaaacgatcc cgatacccgc
     4681 aagctttcga aaaatcacat ctgcccccag acgattcgag ttatcccgat gctctaccaa
     4741 cagcacggga atgtcaatga ttcggcccgg cgggggaaat ttccgggctc tccactaacc
     4801 gccttgcgtc ggcgttagag ttgtggcatg cagatagggg gaacgctgat taatggtctg
     4861 gtccgtgtgc tggtcgtggc cggcaccctg gcggcggtct actacttcgt cgtgcgcccg
     4921 gtgctggaca cgaccgagaa ggtttcgggc gggatcaaca gcagcatcca gcagaccctg
     4981 gacgaagcaa acgacgcctt cgaccagacc aacgtcacga ccaaaaacca gaaggtcctg
     5041 accaggaaga tcaagaacgt cccgacttcg aatatcccga agctcgaaaa ctgcatcacc
     5101 gccgccggca cgaacatcaa ccagatcaac cgctgcatca accgttacgc aaactgactg
     5161 ccggcccgtc gtaaccggtc ccgcatcctc tggcggtacc cttcttcctg ttcagaacac
     5221 atacgcctaa ttcgccgctt ctgcggcgga gcgggggaac cattggtcac cgggcaaccg
     5281 gtggccgggg gcgaatctga ccatctggtc cgtagcgcag cttcttcagc gcgagcccga
     5341 cagctaaccc cgcaggcatg acgaagaagg aggtacccat ggatctggac agacatgtgg
     5401 tgagaccgat gaccgaggaa accggcgccc gcgccgcctt cggcatgctg gtcggagccc
     5461 tcgccgggac agccattgcg ctgcccctgc tggtcggagt cttcggactc taggcctgga
     5521 cggcccgacg cggccgccgc gcttctcgcg cggcggccgc agggccttcc gcccgggctg
     5581 gctacccacc cgggcgccat gagggcgtgg cgaggacctc tcggaggccc ggcgccactc
     5641 cctctagccc cgaacccgta tgaccacagg tttcgaggcc gccccgctgt aggggaagcg
     5701 ggacgagggg acgaggagcg cccgcagcct g
//
LOCUS       DAOQKN010000162         5716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_7390_length_5716_cov_5.737149, whole genome shotgun sequence.
ACCESSION   DAOQKN010000162 DAOQKN010000000
VERSION     DAOQKN010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5716
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7390_length_5716_cov_5.737149"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="PLJ64_09995"
     CDS             <1..563
                     /locus_tag="PLJ64_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNC93855.1"
                     /translation="SGILGTAAAEVIGVDANPEAFEHASAKYTTDRVTFRRELVESFS
                     EPRDAIVFLQTIEHVQDPDSLVAGFAKTAPVAYISTPNLLTLAPEGAEKSDNPWHIKE
                     YRLEEFRALLEPHFARVEILGLHHAGKLKVHELALSLGWDRVHKSLGLSKPFYDRFTP
                     AIDASDFRLSNHNLESSLDFLAVCHV"
     gene            556..1971
                     /locus_tag="PLJ64_10000"
     CDS             556..1971
                     /locus_tag="PLJ64_10000"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HNC93856.1"
                     /translation="MSEGKTLPGAGDLAIVLHSHMPYVEGFGTYPFGEEWLFDAVIRS
                     HLPVLEVARDLTMTVTPVLADQLEAEGVSERLAGFLQEFRLGAARRDVETADPDHVPA
                     TRAEVDRYEKAAVLLERAGGSPLAAFAAVRDRNVSLIPSSATHAVLPLLATRAGLDLQ
                     IDAGLASHRRRFGPSSGFWLPECAYRPGLEDALARHRIEWFCVDQSRHESGDAALAPV
                     RTPAGPLAFTIDWPAISWLWDMSGYPSWGGYLDFHRLSMNGVRLFAIDGGPYEPEAAR
                     QRADRQAGEFLDSVRERLEEYRQRTGQRGLMTFAIDTELLGHWWSEGPDWLAAVLDQA
                     GERGVRAVNLEQAAREHGPSARQSSLKASTWGEAKDFHTWDSPAVADLAWASRGLELR
                     VGEAIGSLPREQAERAVRELLMVQSSDWAFLDHRGQAGDYPFERATGHAESAFEALQS
                     PIGSRLRGLAPDLSLTPFLTP"
     gene            1987..3183
                     /locus_tag="PLJ64_10005"
     CDS             1987..3183
                     /locus_tag="PLJ64_10005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNC93857.1"
                     /translation="MPRILIVSWEYPPLIEGGLARHVRKLSEALVGLGVEVHVLTRGG
                     EQATIEEEMAGVQIHRIVEPERPTDLAEFVTWVERMNADMLAAGVELGDRFDFDLVHG
                     HDWLVAGACDHLARRFNAPLVTTIHATEFGRHQGWVDKHPQSHIHGVEKWIVNRSDRV
                     IACSHFMREQIADIFGVEEGRISVIPNGIDPDDLPAEDPVELARLRAQFADPDENLVL
                     LIGRLVYEKGFQLALEAMPAVIEKLPNTRFLVAGSGTHEAELHKQAKDLGLMEHGTFL
                     GWIGDDVLHSLYRIADLTVVPSIYEPFGLVALEAMASECPCIAADTGGLREVVPIEGA
                     GLRFRASDPEHLAEAAIQVLGDPELSKRMIEEGLAHIRRFDWDEIGEQTLDLYEGLVA
                     STDVRG"
     gene            3194..4171
                     /locus_tag="PLJ64_10010"
     CDS             3194..4171
                     /locus_tag="PLJ64_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93858.1"
                     /translation="MKRLWGSLIVLLAFALAFTATSQAAFSVSYTDRGSLWLSSLDGK
                     HKRKLAAKPNGKVKWIETAQSDNGRVIAVRRDPAKIANLNSYSLWGPTGRRLKFGSLT
                     AESGWTSYVMPLSLDLTSDGKTLVYGYQYFTYNYPVSSLENGTSVKSVNASFLNPINI
                     VGELWPTTVGTRIVASQQDVFAGVQKASSAPYGDEFHGWFDVDGTGYDLHRTDVAANK
                     KVAAAELDPSASDSKQPSLLFASRISGLGGSISGACFLPTKGDAESVSISQDGKSIAW
                     KDNRGVVVAGVPTFGGTDVCKLTRGARVISRTGEFPSIGPAKVSVRKRR"
     gene            complement(4151..4816)
                     /locus_tag="PLJ64_10015"
     CDS             complement(4151..4816)
                     /locus_tag="PLJ64_10015"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNC93859.1"
                     /translation="MAGSLSSFARKGMLKPKYARLFWRYLWRRLLTGSGHRWETDGPV
                     FFGRGLHLQTGRRARIRFGRWVWIGDGTKIRCHEGEVEIGPKTVFGQECTISAYRRVR
                     IGAECVIADRTMFIDFDHGVVDVEKPIRDQGIYMEDVIIGSNVWIGYGAAILRGVRVG
                     DNSVIGTNSVVTRDVPANAVVGGVPARILRMREAPQDLKWPDPVEPEPGTPGVMALPS
                     FSD"
     gene            4866..>5716
                     /gene="meaB"
                     /locus_tag="PLJ64_10020"
     CDS             4866..>5716
                     /gene="meaB"
                     /locus_tag="PLJ64_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HNC93860.1"
                     /translation="MNDAPITMSPLAERLLDGDKRALARAITLIESDDPAGWDLVREV
                     YPKTGKARIIGFTGPPGVGKSTLIGALTKELRKEDRQVAVLSIDPSSPFTQGALLGDR
                     IRLTEHFLDQGVFIRSMASRGALGGLSEAALQAALVMDASGKDDVLVETVGVGQGEID
                     IVDHADTIVLALMPGSGDSIQALKAGVMEIPDIIVVNKSDHPMTDTMIREVRGALSLA
                     HTPGGWRVPILRTEAARGEGIEELAAKVAEHRAFIENEGTLAERRARNLRSEVLGLAT
                     ARLRRRL"
BASE COUNT          957 a         1906 c         1847 g         1006 t
ORIGIN      
        1 gctcgggcat tctcggcact gccgccgccg aggtgatcgg cgtcgacgct aaccccgaag
       61 ccttcgagca cgccagtgcc aagtacacga cggacagggt cactttccgg cgggaacttg
      121 tcgagagctt cagcgagccg cgcgatgcga tcgtcttcct gcagacgatc gaacatgtcc
      181 aggatccgga ctcactggtc gccggctttg cgaagaccgc accggttgcc tacatctcga
      241 ccccgaacct gctcacgctc gcacccgagg gggcggagaa gtcagacaac ccctggcaca
      301 tcaaggagta ccggctcgag gaattccggg ccctgctcga acctcatttc gcccgggtcg
      361 agatcctcgg tcttcaccac gcaggcaagc tcaaggtcca tgaactggca ctgtcgctcg
      421 gctgggaccg cgtccacaag tcgctcgggt tgagcaagcc gttctacgac cgcttcaccc
      481 cggcgatcga cgcctcggat ttccgcctct ccaaccacaa cctcgaaagc tcgctggact
      541 tcctcgctgt ctgtcatgtc tgaaggaaaa accctccccg gggcgggcga cctggcgatc
      601 gtccttcatt cgcacatgcc ctacgtcgag ggtttcggca cctatccctt cggggaagag
      661 tggcttttcg acgcagtcat ccggtcgcac ctgccggtgc tcgaggtcgc ccgtgacctg
      721 accatgaccg tcaccccggt actggccgac cagctcgaag ccgagggggt ttcggagcgg
      781 ctcgcgggtt ttcttcagga attccggctg ggcgccgccc ggcgggatgt tgaaacggcg
      841 gatccggatc acgtgccggc gacccgggcg gaagtcgacc gttacgagaa ggcggcggtc
      901 ctgctcgagc gggccggcgg cagtccgctg gccgcgttcg ccgcggtccg ggaccggaat
      961 gtttccctga tcccctccag cgccactcac gcggtcctgc ccttgctggc gacccgggcc
     1021 gggctcgacc tccagatcga cgccggtctc gcctctcacc ggcgccgttt cggcccttca
     1081 tccggattct ggcttcccga atgtgcctat cgtccgggtc tggaggatgc tcttgcccgg
     1141 caccggatcg agtggttctg cgtcgaccag agccggcacg aatccggcga cgccgccctc
     1201 gcgccggtcc ggaccccggc cggtccgctc gccttcacga tcgactggcc ggcgatctcc
     1261 tggctttggg atatgtccgg ctacccgtcc tggggcggct acctggactt ccatcgcctt
     1321 tccatgaacg gggttcgtct ctttgcgatc gatggtggtc cctacgaacc ggaggcagcc
     1381 cgccagcggg cggaccggca ggcgggcgag ttcctcgact cggtccggga gcgccttgag
     1441 gagtaccgac agcgcaccgg gcagcggggg ctgatgacct ttgcgatcga caccgaactg
     1501 ctcggacatt ggtggtcgga gggtccggac tggctggccg cggtccttga ccaggccggg
     1561 gagagggggg tccgtgccgt caacctcgaa caggcggcga gggaacatgg cccgtcggcc
     1621 cgccaaagca gtctgaaagc ttcaacctgg ggcgaggcta aggacttcca cacctgggat
     1681 tcgcctgccg tggccgacct cgcctgggcc tcgcggggcc tggagctgag ggtgggggag
     1741 gcgatcgggt ccctgccccg ggaacaggcc gagcgggccg ttcgcgagct cctgatggtt
     1801 cagtccagcg actgggcctt cctcgaccac cgcggccagg ccggcgacta tcccttcgag
     1861 cgggccaccg gccatgcgga aagcgccttc gaagccttac aatccccgat cggttcgcgt
     1921 ttgcgcggcc ttgctccaga cctgagtctc acaccgtttc tcacgcccta gccttcagcc
     1981 acccccttgc ctcgcatcct catcgtctcc tgggagtacc cgccgctgat cgagggcggc
     2041 ctggcccggc atgtgcgtaa gctctccgaa gcactggtcg ggctcggggt cgaggttcac
     2101 gtgctgaccc gcggcggtga gcaggcgacc atcgaggaag agatggccgg ggtccagatc
     2161 caccggatcg tcgaaccgga acgcccgacc gacctggccg agttcgtgac ctgggtcgag
     2221 cgcatgaacg ccgacatgct cgccgccgga gtggagctcg gcgaccgttt cgattttgat
     2281 ctggttcacg gtcacgactg gctggtcgcc ggtgcctgtg atcacctcgc ccggcgcttc
     2341 aacgctccgc tggtcacgac gatccacgcg accgagttcg gccgccacca gggctgggtc
     2401 gacaagcatc cccagtcgca catccacggg gtggaaaagt ggatcgtcaa ccgttccgac
     2461 cgggtgatcg cctgttccca tttcatgcgt gaacagatcg ccgacatctt cggcgtcgag
     2521 gaaggccgga tctcggtgat cccgaacgga atcgaccccg acgacctgcc ggccgaagac
     2581 ccggtcgagc ttgcgcgctt gcgggcgcag ttcgcggatc cggatgagaa cctggtcctg
     2641 ctgatcggcc gcctcgtcta cgagaagggt ttccagcttg cgctggaggc gatgccggcg
     2701 gtgatcgaga agctgccgaa cacccggttc ctggtcgccg ggtccggaac ccacgaggct
     2761 gaacttcaca agcaggccaa ggacctcggc ctgatggaac acggcacctt cctcggctgg
     2821 atcggggacg acgtgcttca ttcgctctac cggatcgccg acctgacggt cgttccgtcg
     2881 atttacgagc cctttggtct ggtcgccctc gaagcgatgg cttcggaatg tccatgcatc
     2941 gccgccgaca ctggcggctt gcgggaggtg gtgccgatcg agggcgccgg ccttcgcttt
     3001 cgtgcgtcgg acccggaaca cctggccgag gccgcgattc aggttctcgg tgaccccgaa
     3061 ctcagcaagc ggatgatcga agagggtctc gcccacatcc gccgttttga ctgggatgag
     3121 atcggcgagc agacgctcga tctctacgaa ggactggtcg cttcgaccga cgttcgcggc
     3181 tagtatcagg gccatgaaga ggctatgggg gtccctgatc gtgttgctgg cgttcgctct
     3241 ggcgttcacc gcgacttcac aggccgcttt ctcggtttcc tacaccgacc ggggcagcct
     3301 ctggctttcc tcgctggacg ggaagcacaa gcgcaagctc gccgcgaagc cgaatggcaa
     3361 ggtcaagtgg atcgagaccg cccagtcgga caacggcagg gtgatcgccg tccgtcgcga
     3421 cccggccaag atcgcgaacc tgaactccta ctccctgtgg gggccgaccg gaaggcggct
     3481 caagttcggc tcgctgaccg ccgaatccgg ctggacatcc tatgtaatgc cgctcagtct
     3541 cgacctgacc tcggacggca agaccctcgt ctacgggtac cagtacttca cctacaacta
     3601 cccggtttcg agcctcgaga acggaaccag cgtcaagtcg gtcaacgcct ccttcctcaa
     3661 cccgatcaac atcgtcggcg agctgtggcc gaccacggtc ggcacccgga tcgttgcttc
     3721 gcagcaggac gtgttcgccg gtgtgcagaa ggcgagcagc gccccttacg gcgacgagtt
     3781 ccacggctgg ttcgatgtcg acggcacggg atacgacctt caccggaccg atgttgccgc
     3841 caacaagaag gtcgcagcgg ccgaactgga cccgtccgca agtgattcga agcagccgtc
     3901 gctgctcttc gcttcacgga tcagcggtct gggcgggtca ataagcggtg cctgcttcct
     3961 gccgacgaag ggcgatgccg agagcgtgag catctcccag gacggaaagt cgatcgcgtg
     4021 gaaggacaac cgcggtgtgg tggtggccgg ggttcccacc ttcggtggca cggatgtctg
     4081 caagctgacc cggggcgcca gggtcatctc ccggaccggc gagtttccct cgatcggccc
     4141 ggcgaaggtc tcagtcagaa aacgacggta gggccatcac gccgggagtg cccggttcgg
     4201 gttcgaccgg gtccggccac ttcagatcct gcggggcttc ccgcatcctc agaatccggg
     4261 ccgggacccc gccgactacc gcattcgccg gaacgtccct ggtgactacc gagttcgttc
     4321 cgatcaccga gttgtcgccg accctgaccc cgcgcaggat cgcggcgccg tagccgatcc
     4381 acacgttcga accgatgatc acgtcctcca tgtagatgcc ctggtcgcgg atcggctttt
     4441 cgacgtcgac gaccccgtga tcgaagtcga tgaacatggt ccggtcggcg atcacgcatt
     4501 cggccccgat tcggacccgg cgataggccg agatcgtgca ttcctggccg aagacggttt
     4561 tcggaccgat ctcgacctcg ccttcgtgac aacggatctt ggtgccgtca ccgatccaga
     4621 cccaacggcc gaacctgatc cgggccctgc gtccggtctg gagatgaagc ccccggccga
     4681 agaagaccgg gccgtcggtt tcccagcggt gccccgaccc ggtcaggagc cggcgccaga
     4741 ggtaacgcca gaagagccgg gcgtacttgg gtttcagcat gcccttgcgg gcgaaggatg
     4801 agagtgagcc agccaaaccg gccgtgactc taaccggagc cgcaggttct agactggccg
     4861 ggccgatgaa tgatgccccg atcaccatga gccccctggc cgaacgcctg ctcgatggcg
     4921 acaagcgggc cctcgcccgg gcgatcacgc tgatcgaatc cgatgaccct gccgggtggg
     4981 atctggtccg cgaggtctac ccgaagaccg gaaaggccag gatcatcggg ttcaccggtc
     5041 cgcccggggt cggcaagagc actttgatcg gcgccctcac caaggagctc cgcaaggagg
     5101 accgccaggt tgcggtcctt tcgatcgacc cgtcgagccc gttcacccag ggtgccctgc
     5161 tcggtgaccg aatccgcctg acggagcact tcctcgacca gggtgtgttc atccggtcga
     5221 tggcatcccg cggggcgctc ggcgggcttt ccgaggcagc gcttcaggct gccctggtca
     5281 tggacgcttc cggcaaggac gacgtcctgg tcgagacggt cggggtgggg cagggggaaa
     5341 tcgacatcgt cgaccatgcc gacacgatcg tcctcgccct gatgcccggc tccggtgatt
     5401 cgattcaggc gctgaaggcc ggtgtcatgg agatcccgga catcatcgtg gtcaacaaat
     5461 ccgaccaccc gatgaccgac acgatgattc gcgaggtgcg cggcgcgctc tcgctcgccc
     5521 acacaccggg tggctggcgg gtgccgattc tccgcaccga ggcggctcgc ggcgagggaa
     5581 tcgaagaact tgccgccaaa gtggcggaac accgggcctt catcgagaat gaagggacgc
     5641 tggccgaaag gcgggcccgc aatctgcgct cggaggtgct ggggctggcc acggccaggt
     5701 tgcgtcggcg gctcga
//
LOCUS       DAOQKN010000163         5647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_7515_length_5647_cov_4.658262, whole genome shotgun sequence.
ACCESSION   DAOQKN010000163 DAOQKN010000000
VERSION     DAOQKN010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5647
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7515_length_5647_cov_4.658262"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(372..1724)
                     /locus_tag="PLJ64_10025"
     CDS             complement(372..1724)
                     /locus_tag="PLJ64_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNC93861.1"
                     /translation="MADQLFDSGEVDPGGSGIPQVGPDAPLAARMRPVSLDEVVGQDH
                     ILAPGTALRGAVDAGRPHSAILYGPPGSGKTTLARIIAATSDGAFEEESAVNAGRAEI
                     RAVIARAEDRQRHGRRTVLFLDEIHRFNKAQQDALLPAVESGVLTLIGATTENPYFEV
                     NSALISRSQIYELKALGPEEIRILLERALADSDRGVAGQVEVDPEALGVLSERVSGDA
                     RAALSALERLAAAGGRAGIPEVEDALQAQAIAYDKGGDHHYDYISAWIKATRGSDPDA
                     SLYYLAVMIEGGEDPRFIARRMVILASEDIGNADPQAIVVATAAAAAVDRVGMPEAAL
                     NLAQAATYLALAPKSNASYAAINAAREEVKKNGPKLPPAHLRDSHYPGAKELGRGEGY
                     VYPHDVDGGVSEQSLMPEGLEGLRFYEPTRYGFESELARRQEAAAKIRGKSNKQAKNP
                     "
     gene            1747..2508
                     /locus_tag="PLJ64_10030"
     CDS             1747..2508
                     /locus_tag="PLJ64_10030"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNC93862.1"
                     /translation="MSPVEIAILLVWSFTVSFAGGMVGLVLGNLRLPVVVWLGTSTAA
                     AAGANVAISGLAALTATLVHIRNGRLDPRLFLWMAPSSFLGAVAGGVLAGVLPERILL
                     GAISLVVLYGAVEVWRYRRPKGDGEGNRPDRRRLLTEAVLIGFGVGLLGGFVGLILGS
                     LRLPAMLKYMGMSPQKAVGTNSAVGVIVGIGGLLGHLPSGVDWNILLIGAAAAVPGAA
                     FGSKLTGKLPEATLVRAMAFVLLVTGLAMAGQAII"
     gene            2515..4176
                     /locus_tag="PLJ64_10035"
     CDS             2515..4176
                     /locus_tag="PLJ64_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93863.1"
                     /translation="MDSTGREQLKRHLPEIILGLLMLAQASFLLVLTAPIAFVSDEWQ
                     FLFFRQGFSPGTTLDPYYEHLVAVPNYLYSTAVGIFGLGTNRPMQIAALLTFLGLNAL
                     LFVYLRRRAGGWAALIGTALILFLGAAFEDLLFAFQINYFGAIACGLGALLALDRDDR
                     RGDLIASLLLVAGVLTSSMIFPFIAAAALEWALNPRDRRRRLFVPGAAIGIWILWWIG
                     WGHQADSQAGLSGLSGLPGYLWKAVPAGFTSLAGLATDDGTMPDQPHLIWGRLLCLAA
                     LAAAGWRIYRLGRVPRELWVAIAAAATFFVLSGLNASDFRLPTSSRYQLPSALFIVLI
                     AANLLRGVKIPGRALVASAILAALAVFGGARLMEEQATERWQPTSRSARAALGAIDLA
                     GPAVIPGYQIDLRTNPEQDRLIPIDLYRETTASHGSPGMDASEIEGAAPQYRLQADLA
                     LIGATGIHLSGLPVRPSGPCQRVRGSNNLFGPVPLQSGGYLIMNRGKDQLAVALSRFS
                     DLPGVSAGAILPETMAGLDLPQDNSTRPWQAVFFGEGPVRICPTG"
     gene            4202..>5647
                     /locus_tag="PLJ64_10040"
     CDS             4202..>5647
                     /locus_tag="PLJ64_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93864.1"
                     /translation="MRNPGQSRFLPWVLLGLLMLGSGAILLSVTSGINFVTDEWNLLF
                     LRSGWTPDSFLEPFHEHVIIAPTLIYKVLQGTLGMDSNRPFQLAALIMFLAIGPLLFI
                     WMRRRIGDWAALIGVALILFLGAAFEDLLWAFQIGYFGSLAFGLAALIALDRDDRRGD
                     VAAAILLVLSLSFSSLGLPFVAGAAAEWLTNPRERGKRWLVPAAAAAFYGIWWLGWGH
                     SAETTFSLANIPEAPRFIFDAASAGATSLFGLATGDGSEPDQPHLIWGRIILVALLAL
                     SGLRLWRLKRIPPGVIIAATVALSFFALSALAQSESRLPTSSRYQLPGALFLLLFFTE
                     ILRGLKLKPPALVAGAAVTIAAAVMGVGLMSDQSRDRWVPVSTSTSTYLGMVAYAGEA
                     NDPELVLNLGPGSEVPIDRFLDQVKTSGSPGYSAGEIQSRDAGVRAAADQDLIELTKP
                     LLSGFNPGGNFDRCREYPPDVTVAVRSGVRVI"
BASE COUNT          782 a         1946 c         1883 g         1036 t
ORIGIN      
        1 ggatctcggc gacctcgtcg acgatgccct ggacctggtc cggggtgatg gtttcgactg
       61 tgccgacgag ctcgccggtg gccgggttga aggactccag cggaggagtc gaagcggacg
      121 cggatccgtt gggtgagctg gactcggcgg acggagcggt ttcggtagcc atgaaccgaa
      181 gtgtacgccg gaatggccgg ggcgcaggga gcgcgccccg gctcaccccg gagctgcaaa
      241 caactcggcc ggagaccccc agtccgagtc tccggccgac acagcacccc acctggcgtc
      301 ttgctcaagc tgggtctggc atatgaaacc cggcaaagct caagaagttg tgggaaccgg
      361 ctaaaggttg cttaagggtt tttggcttgt ttattggact tgccgcgtat cttcgcggcg
      421 gcttcctggc gcctcgcgag ttccgactcg aagccgtagc gggtcggctc gtagaaccgc
      481 aaaccttcga ggccctccgg catcagcgat tgctccgaaa cgccaccgtc gacgtcgtgg
      541 ggatagacat acccctcgcc ccggccgagt tccttcgcgc cggggtagtg gctgtcgcgc
      601 aggtgggccg gcggcagctt cgggccgttc tttttgactt cctcccgggc ggcgttgatc
      661 gcggcgtagg aagcgttcga tttcggggcc agcgcaagat aggtggcggc ctgggcgagg
      721 ttgagcgccg cttccggcat gccgacccgg tccaccgcgg ctgccgcggc cgtcgccacc
      781 acgatcgcct gcgggtcggc gttgccgatg tcctccgagg cgaggatcac catgcgccgg
      841 gcgatgaacc tcggatcctc cccgccctcg atcatcaccg ccaggtaata gagcgaggca
      901 tccgggtccg acccgcgggt cgccttgatc caggccgaga tgtagtcgta gtgatgatcg
      961 ccacccttgt cgtaggcgat cgcctgggcc tggagggcgt cctccacttc agggatcccg
     1021 gcccggccac cggctgccgc cagtcgctcc agggccgaga gtgcggcccg ggcatcaccc
     1081 gaaacccgtt ccgagaggac acccagggcc tcggggtcaa cctcgacctg cccggcaaca
     1141 ccgcggtcgg aatcagccag ggcccgctcc agaaggatcc tgatttcctc cggaccgagt
     1201 gccttcagct cgtagatctg gctgcgcgaa atcagggccg aattgacctc gaagtagggg
     1261 ttctccgtgg tcgccccgat cagggtcagc accccgctct cgaccgccgg cagcaatgcg
     1321 tcctgctggg ccttgttgaa gcggtggatc tcgtccagaa agagcacggt gcggcgaccg
     1381 tgcctctggc ggtcttccgc cctggcgatc accgcccgga tctcggcccg gccggcgttg
     1441 accgccgact cctcttcgaa ggcgccgtcg gaggtggccg cgatgatccg ggccagggtc
     1501 gtcttgcccg agccaggcgg cccgtaaagg atcgcgctgt ggggacgacc ggcgtccacc
     1561 gcaccccgca atgcggtacc cggagcgagg atgtgatcct gcccgaccac ctcgtccagc
     1621 gataccggtc gcatccgggc cgccaggggc gcgtccggcc cgacctgcgg aatgccggaa
     1681 ccgccgggat ccacttcacc tgaatcaaac aattggtcgg ccaccgcatg agattattcc
     1741 cggttggtga gtccggttga aatcgccatc ctgctggtct ggtcattcac cgtctccttc
     1801 gccggtggaa tggtcgggct ggtgctgggc aacctgaggc tgccggtcgt ggtctggctc
     1861 ggcacttcga ccgcggccgc cgccggagcg aacgtggcga tctccgggct ggccgcgctg
     1921 accgcaaccc tggtccacat cagaaacggg cggctggacc cccgcctctt cctctggatg
     1981 gcaccgagct cgttcctcgg cgcggtggcg ggcggtgtcc tcgccggggt gctgccggaa
     2041 aggatcctgc tcggggcgat cagcctggtc gtgctttacg gcgcggtcga ggtctggcga
     2101 taccgccggc cgaaaggcga tggggagggg aaccgccccg accggcgccg gctcctgacc
     2161 gaagcggtgc tgatcggttt cggggtcggg ctgctgggcg gattcgtcgg tctgatccta
     2221 gggtcgctgc ggctgccggc gatgctcaag tacatgggca tgagcccgca gaaggcggtc
     2281 ggcaccaact ccgcggtcgg agtgatcgtc gggatcggcg gcctgctcgg gcaccttcct
     2341 tccggggttg actggaacat cctgctgatc ggcgcggccg cggcggtccc gggtgccgcg
     2401 ttcggttcga agctgaccgg gaagctgccc gaggcgactt tggtccgggc gatggcattc
     2461 gtcctgctgg taacgggact cgcgatggcc gggcaggcga tcatctgacc gtcgatggat
     2521 tcgacgggga gagagcaact gaaaagacac ctgcccgaga tcattctcgg tctgctgatg
     2581 ctggcccagg cgagctttct cctggtgctg accgcaccga tcgccttcgt cagtgacgag
     2641 tggcagttcc tcttcttccg ccaggggttc agccccggca ccaccctcga tccctactac
     2701 gaacacctgg tcgcggttcc gaactacctc tactcgaccg cggtcgggat cttcgggctg
     2761 ggcaccaacc ggccgatgca gatcgcggcc ctgctcacct tcctcggcct gaacgccctg
     2821 ctcttcgtct acctgcgccg ccgggcaggc gggtgggcgg ccctgatcgg cactgccctg
     2881 atcctctttc tcggggctgc cttcgaggat ctgctcttcg ccttccagat caactacttc
     2941 ggagcgatcg cctgcgggct cggcgccctc ctggcgctgg accgggatga ccggcggggc
     3001 gatctgatcg cctcgctgct gctggtcgcc ggcgttctga cctccagcat gatctttccg
     3061 ttcatcgccg ccgccgcgct cgaatgggcc ctcaacccga gggaccggcg ccggcggctc
     3121 ttcgttccgg gcgcggcgat cggcatctgg atcctgtggt ggatcggatg gggccatcag
     3181 gccgactcgc aggccgggct ctccggactg agtggtctgc ccggctacct ctggaaggcg
     3241 gtcccggccg gtttcacctc actggccggg ctggcgaccg atgacggcac catgcccgac
     3301 cagcctcacc tgatctgggg gcgcctgctt tgcctggccg ccctggccgc ggcgggctgg
     3361 cggatctacc ggctggggcg ggtgccacgg gagctctggg tggcgatcgc ggccgccgct
     3421 accttcttcg tgctctccgg cctcaacgcc agtgatttcc gcttgccgac ctccagccgg
     3481 taccagcttc cctcggcgct tttcatcgtc ctgatcgcgg ccaacctgct gcgcggggtg
     3541 aagatccccg gacgcgccct tgtcgcctcg gcgatcctgg ctgcgctggc ggtcttcggg
     3601 ggagccaggt tgatggagga acaggcgacg gaacgctggc aaccgacctc gcgctcggcc
     3661 cgggccgcgc tgggtgcgat cgacctggcc ggtccggccg tgatcccggg ataccagatc
     3721 gacctgcgga cgaatcccga acaggaccgc ctgatcccga tcgatctcta ccgggagacg
     3781 accgccagcc acggatcacc ggggatggac gccagcgaga tcgaaggtgc cgcccctcag
     3841 taccggcttc aggccgacct cgccctgatc ggggcgaccg gaatccatct cagtggcctg
     3901 ccggtccgtc cctccggccc ctgtcagagg gtgcgcgggt cgaacaacct cttcgggccg
     3961 gtccccctcc agtcgggcgg ctacctgatc atgaaccggg gcaaggacca gctggcggtt
     4021 gccttgagcc ggttttccga cctgcccggg gtgagtgccg gcgcgatcct cccggagacg
     4081 atggccgggc tggacctgcc gcaggacaat tcgacgaggc cctggcaggc ggtcttcttc
     4141 ggcgagggcc cggtccggat ctgcccgacc gggtgagcct tccgggagta ggttgaccgg
     4201 catgcggaac ccaggccagt cccgttttct gccgtgggtt ctgctcggcc tgctgatgct
     4261 cggttcggga gcgatcctgc tctcggtcac ctcggggatc aatttcgtca ccgacgaatg
     4321 gaacctgctc ttcctccggt ccggctggac cccggacagc ttcctggagc ccttccacga
     4381 gcacgtgatc atcgccccca ccctgatcta caaggttctc caagggaccc tggggatgga
     4441 ttcgaaccgg cccttccagc ttgctgcgct gatcatgttc ctggcgatcg gcccgttgct
     4501 cttcatctgg atgcggagac ggatcgggga ctgggcggcg ctgatcgggg tcgccctgat
     4561 cctctttctg ggggcggcct tcgaggacct gctctgggcc ttccagatcg gctacttcgg
     4621 gtcgctggcc ttcggtctgg ccgcgctgat cgcactcgat cgcgacgacc gccgggggga
     4681 tgtcgcggcg gcgatcctgc tggtcctttc cctcagcttc tcctcgctcg gcctgccctt
     4741 cgtggccggc gcggccgccg agtggctgac caatccccgt gagcggggga agcgctggct
     4801 ggttcccgca gcagccgccg ccttctacgg catctggtgg ctgggctggg gccacagcgc
     4861 cgagaccacc ttcagcctcg ccaacatccc tgaagccccg cgcttcatct tcgacgccgc
     4921 ctcggccggg gccacttccc tcttcggcct cgccaccgga gacggcagtg aacccgatca
     4981 gcctcatctg atctggggcc ggatcatcct ggtggctctg ctcgccctct ccgggctgcg
     5041 gctctggcgg ctgaagcgga tccctcccgg ggtaatcatc gccgcaaccg tcgcgctctc
     5101 cttcttcgcc ctttccgcgc tcgcccagag tgagagccgc ctgcccacct ccagccgcta
     5161 ccagctgccg ggggctctct tcctgctgct cttcttcacc gagatcctgc gcgggctgaa
     5221 gctgaagccg ccggccctgg tcgccggcgc ggcggtcacg atcgcggccg cggtcatggg
     5281 ggtcgggctg atgagcgacc agtcccggga caggtgggtt cctgtctcga cctcgaccag
     5341 cacctacctc ggcatggtcg cctacgccgg ggaagcgaat gacccggagc tcgtactcaa
     5401 cctcggaccg gggtccgagg tcccgatcga ccgcttcctc gatcaggtca agaccagcgg
     5461 ttcacccggg tattcggccg gcgagatcca gtcccgggat gccggggtga gggccgccgc
     5521 cgatcaggac ctgatcgagc tgaccaaacc gctgctttcc ggcttcaacc ccggcggcaa
     5581 ctttgaccga tgccgcgagt acccgcccga tgtcaccgtt gcggtcaggt ccggcgtgcg
     5641 ggtgatc
//
LOCUS       DAOQKN010000164         5557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_7721_length_5557_cov_5.922210, whole genome shotgun sequence.
ACCESSION   DAOQKN010000164 DAOQKN010000000
VERSION     DAOQKN010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5557
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7721_length_5557_cov_5.922210"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /locus_tag="PLJ64_10045"
     CDS             <1..1018
                     /locus_tag="PLJ64_10045"
                     /inference="COORDINATES: protein motif:HMM:NF016129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HtaA domain-containing protein"
                     /protein_id="HNC93865.1"
                     /translation="TGVRYGKSARVKLGGGLRISHGRRAVRISDLMVVSPAGKPARID
                     GRLGRTRHTLFRVKAGKRIFNAASGKLSKVGSARLTAGGARVLNRNLRLRRSRLKAGL
                     VWGYMNLYSLYKVTQVDDPTGEVPEVPPEKERPIEAKTVASAATVKWFVRDSFINYVA
                     SGEGTRVEDGATADAPSGPDHLVYSFNFPFDSGWTVPEAIDSPENTVIRGTGLVGFRY
                     CHNTINFTAASPVIEIDGDANSRLIFTVHGTDGTAFPEQPAVMVKLIPSRAQSHTVVD
                     NMNGYSTVTWEKIPGYVPAEGTGIFAGFYPAFSPDYEGQPQTERPDRFGYFSITYTFP
                     NTDH"
     gene            1015..2205
                     /locus_tag="PLJ64_10050"
     CDS             1015..2205
                     /locus_tag="PLJ64_10050"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNC93866.1"
                     /translation="MSQVDPSRTSTSGRILAFGAALLATVVGSMSLAAQSPAAIQEGI
                     VEVNGYAWTVNPVAVDLNDIAPTNNGSSRYTVLEILEEGQNGQYRISQIPRVTINLPG
                     KLTGPGCSGDQIRGNSNQCPTFRSTPDATIMSYKAGSQTRQVEYRDYNPQIYIEKADD
                     MSVDLSPPSAKIDSGGQVRFTTDVSGDIGAVTYEWDFGDGTKKTTSKGSISHTFKGSN
                     RTYTVVVSVTSTGNPRSADDLSTITVGKVKKKPADQNPNQKNPDNSDNGGGGETTEST
                     PYVPGYSGGSTGGTGGGSPGASPNTSPGQPKREKQQPANESSGQTVTGQLIDPASIAT
                     ATPSTGDSTAATEQASPDDGSGGGGIPDGAKAALGIGALLGLGGLAEAGAFTGAFRRI
                     RFRL"
     gene            complement(2215..2964)
                     /locus_tag="PLJ64_10055"
     CDS             complement(2215..2964)
                     /locus_tag="PLJ64_10055"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNC93867.1"
                     /translation="MIIQRVEQSEWLSNSYLVCDETGGSGVLVDGNGVIEPLLERVDR
                     EGITLTHILLTHEHWDHVVDLREVADRYGVPIVASQKTADLVDFKVDQIVEDGDETVS
                     GGLTIRWIATPGHSDGHMALLINGTDVITADVIFKGTVGGTVAPGETGFPELKSSIMD
                     RLMTLPPETRIHPGHREPSTVGEEWENNPFIRVWRGLDEEGDDPCEVNNFGPATLILW
                     APDYDGTNKAWIRLPDGEDKITGGSQILSRG"
     gene            complement(3044..4144)
                     /locus_tag="PLJ64_10060"
     CDS             complement(3044..4144)
                     /locus_tag="PLJ64_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003984083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNC93868.1"
                     /translation="MRAAVLEEFGKPLVVQEVDLAEPKEGEVLVRLEACGVCHTDMYT
                     ASGADPSGYAPCVLGHEGGGIVEKVGEGVTSVAPGDKVVTLFSPQCRECEHCLDPRTN
                     LCMAIRAEQNLGHLPDGTTRLSRDGEEIRHFMGTSTFAEYTVMPEIALAKINPEAPLD
                     HACLFACGLSTGLGAAMFTAKVEPGTTCVVFGAGMVGLGAVAGARLMGAERIICVDLS
                     DDRLELARGQGATDLHKADENTVQWILDETGGMGADYTFEATGLVGVMQQAVEAARMA
                     WGLCTIAGVAGKGETLDIIPRWLITGRRVAGSSFGGVKGRDQVPQLVDRYLAGEIDVE
                     PFLSHRISLDEVNKGFDLMEAQDGIRSVIEFT"
     gene            4546..>5557
                     /locus_tag="PLJ64_10065"
     CDS             4546..>5557
                     /locus_tag="PLJ64_10065"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HNC93869.1"
                     /translation="MPFRRLIPLAAAVLLLAIAGCGSEGGDGSSPGGDSASGGSETEQ
                     VKVFTTEGEQFDPVQKEVPAGDLPKAATQALLDAPADRTYIPEGTEVKDVTVTGDGKA
                     TVKLSDEFLGDIPADPAERTRRENDAVEGRVAQVTYTLTSLKSVDQVKVESGGLAVTK
                     TKDRADFREPQKAPPLPGQGDQYGARASGATQLQQQLADLRYLPQSGVDGVIGYQTQQ
                     AVMAFQSVEGLTRDGVAGPVTKAALRVAKTPQPSARRPQRLMEVRIGKGVLLLVRGGK
                     TVRVIHVSAGGPGNETPTGRYRIFRKELQSWSVPFSTWLPYASYFNNGIAFHEYPDVP
                     PYP"
BASE COUNT         1051 a         1882 c         1766 g          858 t
ORIGIN      
        1 gaccggggtc cgctacggaa aatccgcacg agtgaaactc gggggaggcc ttcggatctc
       61 tcacggaagg cgagcggtcc ggatcagcga cctcatggtc gtctctccgg ccggcaaacc
      121 ggcgaggatc gacggccgcc tcggccggac ccggcacacc ttgttccggg tcaaagccgg
      181 caagcggatc ttcaacgctg cgagcggcaa gctctcaaag gtcggatccg cccggctgac
      241 cgccggcggc gcccgggtac tgaaccgcaa cctccgcctc cgccgcagca gactgaaggc
      301 cggactggtc tggggataca tgaacctcta ctcgctctac aaggtgaccc aggtcgatga
      361 tccgaccggc gaggttcccg aagtgccgcc ggagaaggaa cggccgatcg aggcgaagac
      421 cgttgcctcg gcggcgacag tcaagtggtt cgtccgcgac agcttcatca actatgtcgc
      481 cagcggagaa ggaacgagag tcgaggacgg cgccacggct gacgcgccga gcggcccgga
      541 ccatctcgtc tactcgttca acttcccctt cgacagcggc tggacagtgc ccgaggcaat
      601 cgacagcccc gagaacaccg tgatcagggg aaccggcctg gtcggtttcc gctactgcca
      661 caacacgatc aacttcaccg ccgccagtcc ggtgatcgaa atcgatggtg acgccaactc
      721 ccgactgatc ttcaccgtcc acggaaccga cgggaccgcc ttccccgagc agccggccgt
      781 catggtcaag ttgatcccga gccgggccca gtcacacacg gtggtcgaca acatgaacgg
      841 ttacagcacc gtgacctggg agaagatccc cggctacgtg ccggccgagg gcacgggcat
      901 tttcgccggc ttctatccgg ccttcagtcc ggactacgaa ggccagcccc agaccgaacg
      961 ccccgaccgg ttcggttact tctcgatcac ctacaccttc ccgaacacgg accattgagc
     1021 caggtcgatc cgagccggac atcgacttcg gggcggatcc tcgccttcgg ggcggccctg
     1081 ctggcgacag tcgtcggctc gatgtccctg gctgctcaat ccccggccgc gatccaggag
     1141 gggatcgtcg aagtgaacgg atacgcctgg accgtgaacc cggtcgcggt cgacctgaat
     1201 gacatcgcac cgaccaacaa cgggagcagc aggtacacgg tcctcgagat cctcgaggaa
     1261 gggcaaaacg gccagtaccg gatcagtcag atcccccgtg tcacgatcaa tctccccgga
     1321 aagctgaccg gacccggctg ctccggcgac cagatccgcg gcaactcgaa tcagtgcccg
     1381 accttccggt ccacccctga cgcaaccatc atgagctaca aggccggctc gcagaccagg
     1441 caggtcgagt accgggacta caacccgcag atctacatcg agaaagcgga cgacatgtcg
     1501 gtcgaccttt cgccgcccag cgccaagatc gacagcggcg gccaggtcag gttcacgacc
     1561 gatgtcagcg gggacattgg cgccgtcacc tatgagtggg acttcggcga cgggaccaaa
     1621 aagacgactt cgaaaggctc gatctcccac accttcaagg gcagcaaccg gacttacacg
     1681 gtcgtggtca gcgtcacttc gaccggcaac ccccgcagcg ccgatgacct gtccacgatc
     1741 accgtcggca aagtgaagaa gaagccggcc gaccagaatc cgaaccagaa gaacccggac
     1801 aacagcgaca acgggggcgg aggggagacc accgaatcga ccccatacgt tcccgggtat
     1861 tcgggcggca gcacgggcgg aaccggcggc ggcagccccg gggcttcgcc gaacaccagc
     1921 cccggtcagc cgaagcggga aaagcagcag ccggcgaacg aaagcagcgg ccagaccgtg
     1981 accgggcagt tgatcgatcc ggccagcatc gccaccgcga ccccttcgac cggtgactcg
     2041 accgcggcga ccgagcaagc gagcccggac gatggctcgg gcggcggggg catcccggac
     2101 ggcgccaagg ctgcgctcgg gatcggcgcc ctgctcggcc tcggcgggct ggccgaagcc
     2161 ggcgccttca ccggggcctt ccgccgtatc cgcttccggc tctagctcgt cgtctcagcc
     2221 gcggctgagg atctgcgaac cgccggtgat cttgtcttcg ccgtccggca ggcggatcca
     2281 ggccttgttg gtgccgtcgt agtcgggcgc ccagaggatc agggtcgccg ggccgaagtt
     2341 gttgacctcg cagggatcgt cgccctcttc gtcgagaccg cgccagaccc ggatgaaggg
     2401 gttgttttcc cattcctcgc cgaccgtcga tggttcgcgg tggccgggat ggatgcgggt
     2461 ctcgggcggc agggtcatca gccggtccat gatcgagctc ttgagctccg gaaacccggt
     2521 ctcgcccggg gcaaccgtgc cgccgaccgt gcccttgaag atcacgtcgg cggtgatcac
     2581 gtcggtgcca ttgatcagca gggccatgtg gccgtccgaa tgtcccgggg tggcgatcca
     2641 cctgatcgtc agtccgcccg agacggtttc gtccccgtcc tcgacgatct ggtcaacctt
     2701 gaagtcgacc aggtcggcgg tcttctggct ggccacgatc ggcaccccgt aacggtcggc
     2761 gacctcgcgc aggtcgacca cgtggtccca gtgttcatgg gtgagcagga tgtgggtgag
     2821 ggtgatccct tcccggtcga cccgctccag cagaggctcg atcactccgt tgccgtcgac
     2881 cagaaccccc gaaccgccgg tctcgtcgca gacgaggtag gagttggaga gccattcgga
     2941 ctgttcgact cgctggatga tcatgcgcgg tcctttcggg tgggatggaa ggaagagtga
     3001 ctggttttcg gccgggagcg cggcatcggc tgcgggcggg gcatcaggtg aactcgatca
     3061 cgctgcggat gccgtcctgg gcttccatca ggtcaaagcc cttgttgacc tcgtcgaggc
     3121 tgatccggtg ggagaggaac ggttcgacgt cgatctcgcc ggcgaggtag cgatcgacca
     3181 gttgcggcac ctggtcgcgg cccttgacgc cgccgaacga agagccggcg acccggcgac
     3241 cggtgatcag ccagcgggga atgatgtcga gagtctcgcc cttgccggcc acgccggcga
     3301 tcgtgcagag cccccatgcc atgcgggccg cttcgaccgc ctgctgcatc acccctacca
     3361 ggccggtcgc ttcgaaggtg tagtcggcgc ccattccgcc ggtttcgtcg aggatccact
     3421 ggaccgtgtt ttcgtctgcc ttgtggaggt cggtcgcgcc ctggccccgg gccagttcga
     3481 gccggtcatc cgagaggtcg acacagatga tccgctctgc ccccatcagg cgcgccccgg
     3541 cgaccgcgcc gaggccgacc atgccggctc cgaagacgac gcaggtcgtg cccggttcga
     3601 ccttggccgt gaacatcgcc gcgcccagcc cggttgaaag gccgcaggcg aaaaggcagg
     3661 catggtcaag cggagcttcg ggattgatct tggccagggc gatctccggc atcaccgtgt
     3721 actcggcaaa ggtcgaggtg cccatgaagt ggcggatttc ctcgccgtcg cgggaaaggc
     3781 gggtggtgcc gtcgggcagg tggccgaggt tctgctcggc ccggatcgcc atgcagaggt
     3841 tggtccgggg atcgaggcag tgttcgcatt cgcggcactg cggcgagaac agggtcacga
     3901 ccttgtcgcc gggcgccacc gaggtgactc cttcgccaac tttctcgacg atcccgccgc
     3961 cctcgtgacc gagcacgcag ggcgcgtacc cggacgggtc ggcccccgag gcggtgtaca
     4021 tgtcggtgtg gcagaccccg caggcctcca gccggaccag cacctcgccc tctttcggct
     4081 cggccagatc gacctcctgg accacgagcg gtttgccgaa ctcctcaaga acagcagccc
     4141 tcatcctcat aacccgatcc tagagaacca accccccaag tcaaaaccca accaaaaccc
     4201 aagaaccaag cttcaacctc aaccaccaac caccaatccc aaccaagctt caaccccaac
     4261 ccccagccag ggcccaccaa attcccacca aaccaagaaa cccctcccac cagaatcctt
     4321 ccgggggcga tcacccaccg atccagtagc cgaactcccc cgaaaaccag gaagaagcca
     4381 ccaaccaccc cgcccgttcc gggggcgatc acccaccatt tccgtggcca gactcccccg
     4441 gaaggacgaa cctggccagc ctcccgccgt tccgggggcg atcgcccacc gaatccgtag
     4501 ccaatcgccc ccggttccgg tggctggcgg caatagggtg tggccgtgcc gttccgtcgc
     4561 ctgattccgc tcgcagctgc cgtcctcttg ctcgcaatcg ctggctgtgg ctcggagggc
     4621 ggggatggct cgagtcccgg cggtgattcg gcctctggcg gaagcgaaac cgagcaagtc
     4681 aaggtcttca ctaccgaagg cgagcagttc gatccggtcc agaaggaagt acccgccggc
     4741 gacttgccga aagccgcgac ccaggccctc ctcgatgcgc cggcggaccg aacgtacata
     4801 cccgaaggaa ccgaagtgaa agatgtcact gtcacaggtg acgggaaagc gacggtgaag
     4861 ctctccgacg agttcctggg tgacatcccc gccgatccgg ccgagcgcac gcggcgcgag
     4921 aacgacgcgg tcgaggggag ggtcgcccag gtcacataca ccttgacctc gctgaagagc
     4981 gtcgaccagg tgaaagtgga atctggcggc ctggccgtga cgaagaccaa ggatcgggca
     5041 gatttcaggg agccgcagaa ggccccgcca ctgcccggcc agggcgatca gtacggcgcg
     5101 agggcgtccg gggcgactca gctccaacaa cagctggcgg acctccgtta cctgccgcag
     5161 agcggggtgg acggggtgat cggctaccag acccaacagg cggtgatggc gttccagtcg
     5221 gttgagggac tgacccggga cggggtggcc ggtccggtaa cgaaggccgc cctccgagtt
     5281 gcgaagaccc ctcagccgag cgccaggcgt ccgcagcgac tgatggaggt gcggatcggc
     5341 aagggcgtgc tgcttcttgt caggggtggg aagacggtcc gggtgatcca cgtttcggcg
     5401 ggcgggcccg gtaacgaaac cccgaccggt cgctacagga ttttccgcaa ggaactccag
     5461 tcctggtcgg ttccgtttag cacctggctt ccgtatgcct cgtacttcaa caacgggatc
     5521 gccttccacg aataccccga cgtgccgccg taccccg
//
LOCUS       DAOQKN010000165         5520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_7808_length_5520_cov_6.047758, whole genome shotgun sequence.
ACCESSION   DAOQKN010000165 DAOQKN010000000
VERSION     DAOQKN010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5520
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7808_length_5520_cov_6.047758"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..1261
                     /locus_tag="PLJ64_10070"
     CDS             56..1261
                     /locus_tag="PLJ64_10070"
                     /inference="COORDINATES: protein motif:HMM:NF012263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HNC93870.1"
                     /translation="MSLLKRKKTEAAGTNGSGDAEAVTKPTRGERMGQAKELGVHHGT
                     QAGIDVAGWVDSRVNATGIFTAMMSRHVPKGTNWFYTLGSATMFIFAMQAITGVFLSM
                     YYTPSATQAYASITHMTNEVFMGEFVRGMHKWGASLMIILIFLHMARTFFFGAYKYPR
                     ELNWVIGVVLLILTTVMGLTGYLLPFDQRSFWATIVANNITASGPVVGPYLADFLRAG
                     PDFSATTLSRFYALHMMVVPGLIAGLIGAHLFFVVKLGTTAPPWVRAGRPKGARPEEA
                     VTAGVAREDLDEVPVNGAVAAAVAEAEPPVAEADAAEADAVEAREAEETEAAVATEDP
                     AEEAAAEEAAQAPAEQAPVEETPVEEVSTEVPSEESAADETAPEEPPADPADAPDEPE
                     ASGEGEVNS"
     gene            1258..1971
                     /locus_tag="PLJ64_10075"
     CDS             1258..1971
                     /locus_tag="PLJ64_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNC93871.1"
                     /translation="MNAREKEEYLREYEILKKKGKPFFPYAIMKDSVMMLIVAGVLVA
                     MSIIMGAEQGPKADPTTTTYTPRPEWYFFYLFELLRVIKPPALVPVATIGIPTICMVL
                     LLLLPFYDRGPQRVPWKRPIATTTGIMVIIAMALLTYKGAVAGSPSEIELATSPQYEK
                     GKAIVAESGCLACHAIGENGAHGGLGPDLTDIGSKIPKEAIKRSVAVGPGIMPSFQGL
                     GEENLNEVADYLASLDGEE"
     gene            1979..2701
                     /gene="ubiE"
                     /locus_tag="PLJ64_10080"
     CDS             1979..2701
                     /gene="ubiE"
                     /locus_tag="PLJ64_10080"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HNC93872.1"
                     /translation="MNQAPPRRGDQEFAGEVNSMFDRISGVYDRMNRVMTAGLDQSWR
                     ARAAERARLEPGMKALDLCCGTGDLALMLAERVGPEGEVTGADFSRPMLDIALEKAKS
                     EGLRQARFKWADALDLPFEDESFDALTIAFGARNLADLDRGISEMRRVLVPGGRLVIL
                     EITQPKRQPLAGFFALWFDRIVPQLGKLAGDSSAYTYLPESVRSFPDAETLAGKLDAG
                     GLRHVRWTLMAGGIIALHSGVR"
     gene            2714..3709
                     /locus_tag="PLJ64_10085"
     CDS             2714..3709
                     /locus_tag="PLJ64_10085"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNC93873.1"
                     /translation="MGAADEAPRNRSLPEPVTAVTDTAGDWLPARMAEVEAGLLAAVE
                     GETGLAGDAVRTLNAGGKRMRPMLVLLCAGPEAGPAAVRSGIAVELIHMASLVHDDVL
                     DRAPLRRGLPTVYASSGRERATALGDRLFSTAFKVLVDGGDSAAVSEVSATAVDLARG
                     ELRQREGAFDMELTETDYFDRCALKTGRLFEAACVLGRGSTGGDPGPVGLFGHRIGLA
                     FQLLDDVLDIAGPPERTGKARGTDLLDGTVTLPLIFAARFDPALAALDLRTLNEESAA
                     VACDRIAATGALEEVRSRAIELIEEAKAGLIEAGLEQQQRQLLELIADGVVQRYS"
     gene            3737..4303
                     /gene="dcd"
                     /locus_tag="PLJ64_10090"
     CDS             3737..4303
                     /gene="dcd"
                     /locus_tag="PLJ64_10090"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HNC93874.1"
                     /translation="MVLSDKTLREEIEAGRIVIDPFEEKLIQPSSIDLRVDHSYRVFN
                     NSRYPFIDVKQPMEDLTELVKPSDDEPFILHPGEFVLGQTLERVTVPDDMVARLEGKS
                     SLGRLGLLIHSTAGFVDAGFSGNLTLELSNVANLPITIYKGMPIGQLSFMRMDRAVDT
                     AYGADGKGSKYQGQAEPTASRYYLNFRD"
     gene            4365..4691
                     /locus_tag="PLJ64_10095"
     CDS             4365..4691
                     /locus_tag="PLJ64_10095"
                     /inference="COORDINATES: protein motif:HMM:NF011430.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNC93875.1"
                     /translation="MIPNIGPLEIVIVLIIALIVFGPKRLPELGKSLGRGLNEFREGM
                     SNIGNHDDDDEDEDDAEDHEPAELTPPPSDEKLPLPGEEPEVTEVTGSSDAEPVEGEV
                     VPDSRD"
     gene            4699..5469
                     /gene="tatC"
                     /locus_tag="PLJ64_10100"
     CDS             4699..5469
                     /gene="tatC"
                     /locus_tag="PLJ64_10100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNC93876.1"
                     /translation="MARLKPVKHDEHLSLVDHLDELRTRIIYSLGFLAVAFAVCFWQS
                     DVILDVAAAPLPEAHKALVVLAPTEAFMTTVTVCAYAAIIVSAPFISYQVFAYILPAF
                     SPRERRAILPMLFTIPLLFLVGCVFAYFVVLPAAINFLLSFNGEQFAIQLRAREYYSF
                     FSMTLLAGGVVFELPIVILALVRLRILTVDQLRQNRRYAYLICAVIAAALPGVDPISM
                     LIETVPLLVLYELSILLARGIGQPKESAESQIPSIQEN"
BASE COUNT          945 a         1868 c         1778 g          929 t
ORIGIN      
        1 ggggccgctc gagcgctatc tcgggccgga ggtccccgcc gagcagttga tctgaatgag
       61 cctgctcaag cgaaagaaga ccgaagcagc cgggaccaac gggtccgggg atgccgaagc
      121 tgtgaccaag ccgaccaggg gcgagcggat gggccaggcc aaggaactcg gagtccatca
      181 cggcacccag gccggcatcg acgtcgccgg ctgggtcgat tcgcgcgtaa acgcgaccgg
      241 aatcttcacc gcgatgatgt cgcggcacgt gccgaaggga accaactggt tctacacgct
      301 cggttcggcg acgatgttca tcttcgccat gcaggcgatc accggcgtct tcctctcgat
      361 gtactacacg ccctccgcca cccaggcgta cgcctcgatc acccatatga ccaacgaggt
      421 cttcatgggc gagttcgtgc gaggcatgca caagtggggc gcctcgctga tgatcatcct
      481 gatcttcctg cacatggcgc ggaccttctt cttcggcgcc tacaagtacc cgcgggaact
      541 gaactgggtg atcggcgtcg tgctgctgat cctgaccacc gtcatgggtc tgaccggcta
      601 cctgctgccc ttcgaccagc ggtccttctg ggcgaccatc gtcgccaaca acatcaccgc
      661 ttccggcccg gtcgtcggcc cctacctggc ggacttcctg cgagccggcc ccgacttcag
      721 cgccaccacc ctttccaggt tctatgccct ccacatgatg gtcgtaccag gcctgattgc
      781 gggcctgatc ggcgcccacc tcttcttcgt cgtcaagctt ggcaccaccg ccccgccgtg
      841 ggtccgggcc ggccgtccga agggcgcccg tccggaggag gcggtcaccg ccggagtcgc
      901 ccgcgaggat ctcgacgagg ttccggtgaa cggcgccgtc gcggcggccg tggccgaagc
      961 cgagccaccg gtggcggaag ctgacgcggc cgaggccgac gcggtcgagg cccgggaagc
     1021 agaggaaacg gaagctgccg tagcgaccga agacccggcc gaagaagccg ccgccgaaga
     1081 ggcagcccag gcgcccgccg agcaggcccc ggtcgaggaa acaccggtcg aggaggtttc
     1141 gaccgaagtg ccgagtgaag aatcggccgc cgacgagacc gcccccgagg agccgccggc
     1201 cgatccggct gacgccccgg acgaacccga ggcttccggc gaaggggagg tgaactcgtg
     1261 aacgcccgtg aaaaggagga gtacctccgc gagtacgaga tcctgaagaa gaagggcaag
     1321 ccgttcttcc cctacgcgat catgaaggac tcggtgatga tgctgatcgt cgccggcgtg
     1381 ctggtcgcga tgtcgatcat catgggtgcc gaacaggggc ccaaggcgga cccgaccacc
     1441 accacctaca cgccgcgccc cgagtggtac ttcttctacc tcttcgagct gctgcgcgtg
     1501 atcaagccgc cggccctggt tccggtcgcc acgatcggca tcccgaccat ctgcatggtc
     1561 ctgctgctgc ttctgccctt ctatgaccgt ggaccccagc gggtgccgtg gaagcgtccg
     1621 atcgcgacca ccaccggcat catggtgatc atcgcgatgg cgctgctgac ctacaaggga
     1681 gcggtcgccg gctcgcccag cgagatcgaa ctcgccacct cgccgcaata cgagaagggc
     1741 aaggcgatcg tcgccgaatc cggctgtctg gcctgccacg cgatcggcga gaacggggcc
     1801 cacggcggtc tcggtccgga cctgaccgac atcgggtcga agatcccgaa ggaagcgatc
     1861 aagcgttcgg tcgcggtcgg cccgggcatc atgccctcct tccagggtct cggtgaagag
     1921 aacctgaacg aggttgccga ctacctcgct tcgctcgacg gcgaagagta gccgtccaat
     1981 gaaccaggca cctccccgcc ggggtgacca ggagtttgcc ggcgaggtga actcgatgtt
     2041 cgaccggatc agcggtgtgt acgaccggat gaaccgggtg atgaccgccg gcctcgacca
     2101 gagctggcgg gcgcgcgcgg ccgaaagggc ccggctcgaa cccggcatga aggcgctcga
     2161 cctttgctgc ggcaccggtg acctcgccct gatgctggcc gaaagggtcg gcccggaagg
     2221 tgaagtgacc ggcgcggact tctcccgccc gatgcttgac attgcgcttg agaaagccaa
     2281 aagcgaaggg ctgaggcagg cacggttcaa gtgggccgac gccctcgacc tgcccttcga
     2341 ggatgaatcc ttcgacgcct tgacgatcgc cttcggcgcc cgcaacctgg ctgacctcga
     2401 ccggggaatc agcgagatgc gccgggtcct ggtgccgggc ggtcgcctgg tcatcctcga
     2461 gatcacccag ccgaagcgcc agcccctggc cggcttcttt gccctctggt tcgatcggat
     2521 cgtgcctcag ctcggcaagc tcgcgggtga ctcctccgcc tacacctacc tgccggaatc
     2581 ggtgcgcagt ttccccgatg cggagaccct ggccggcaag cttgacgccg ggggcctccg
     2641 gcatgtccgc tggactttga tggcgggcgg gatcatcgcc cttcactccg gggtccggtg
     2701 agatagctcg gccatgggcg cggctgacga agccccccgg aaccggtcgc tcccggagcc
     2761 ggtcaccgct gtaaccgaca ccgccgggga ctggctaccg gcccgcatgg cggaggtcga
     2821 agccggactg ctggccgcgg tcgaagggga gaccgggctg gccggggacg cggtccgcac
     2881 cctcaacgcg ggcggcaagc gcatgcgccc gatgctggtc ctgctttgcg ccggcccgga
     2941 ggcaggcccg gctgcggtcc gttcggggat cgcggtcgag ctgattcaca tggccagtct
     3001 ggtccacgat gacgtgctcg accgggcgcc gctacggcgc ggactgccca ccgtctacgc
     3061 cagctcgggc cgggaacggg cgaccgccct gggtgaccgt ctgttctcga cggcattcaa
     3121 ggtcctggtc gacggaggtg attccgccgc cgtctccgag gtcagcgcga ccgcggtgga
     3181 cctcgcccgc ggcgagctca ggcagcgcga gggggccttt gacatggagc tcaccgagac
     3241 cgactacttc gaccgctgcg ccctgaagac cggccggctc tttgaagcgg cctgcgtgct
     3301 cggccggggc agcaccggcg gtgatccggg gccggtcggg ctcttcggcc accggatcgg
     3361 tctcgccttc cagctgctcg acgacgtgct cgacatcgcc ggcccgccgg agcgaaccgg
     3421 caaggcccgg ggcaccgacc tgcttgacgg cacggttacc ctgccgctga tcttcgccgc
     3481 ccgcttcgac cccgccctgg ccgcgctgga cctccggacc ctgaacgaag aatcggcggc
     3541 cgtcgcctgt gaccggatcg cggccaccgg cgcgctggag gaggtccgga gccgggcgat
     3601 cgaactgatc gaggaagcca aggccggtct gatcgaagcc ggtctcgagc agcagcagcg
     3661 acagctcctg gagttgatcg ccgatggagt cgtccagaga tacagctgac cggctgctct
     3721 gggatactgc cgcttcatgg tcctgagtga caagaccctg cgggaggaga tcgaggccgg
     3781 acggatcgtg atcgatccct tcgaggagaa gctgatccag ccctcgagca tcgacctccg
     3841 cgttgaccac agctaccggg tcttcaacaa ctcgcgctac ccgttcatcg acgtgaagca
     3901 gccgatggag gatctgaccg agctggtcaa gcccagcgat gacgagccct tcatcctcca
     3961 cccgggcgag ttcgtcctcg gccagaccct ggagcgggtc acggtcccgg atgacatggt
     4021 cgcccgcctg gaaggcaaga gctcgctcgg ccggctcggt ctcctgatcc attcgaccgc
     4081 gggtttcgtc gacgcgggat tctccggcaa cctgactctc gagctctcca acgtggccaa
     4141 cctgccgatc acgatttaca agggcatgcc gatcggccag ctctccttca tgcggatgga
     4201 ccgggcggtt gacacggctt acggagccga cggcaagggt tcgaagtacc agggccaggc
     4261 cgaacccacc gcctcgcgct actacctgaa tttccgggac tgaactcctt caccgcccgg
     4321 tgatccgggc ccgccggcgg ttcgtaggta cgattggtgt ccagatgatt ccgaatattg
     4381 gcccgctcga aatagtcatc gtcctgatca tcgcgctgat cgttttcggc ccgaagcgcc
     4441 tgcccgaact cggcaagtcg ctgggccggg ggctgaacga gttccgcgaa ggcatgagca
     4501 acatcggaaa tcacgacgat gacgacgaag atgaagatga cgcggaagat cacgagccgg
     4561 ccgaactgac gccacccccg agtgacgaga agctcccgct gcccggggaa gagcccgagg
     4621 tcaccgaagt gaccggcagc tccgacgccg agccggtcga gggtgaagtg gttcccgact
     4681 cacgggactg aggccgctgt ggccaggctc aaaccggtca agcacgacga gcatctgagt
     4741 ctcgtcgacc atctcgatga actgcgtacg cggatcatct actcgctggg tttcctagcg
     4801 gttgccttcg cggtctgctt ctggcaaagc gacgtgatcc tcgacgtcgc ggccgccccc
     4861 ctgccggagg cgcacaaggc cctggtcgtg ctggccccga ccgaagcctt catgaccacg
     4921 gtgaccgtct gcgcctacgc ggcgatcatc gtctccgccc ccttcatcag ctatcaggtc
     4981 ttcgcctaca tcctgccggc cttctcgccc cgcgagcgca gggcgatcct gccgatgctg
     5041 ttcacgattc cgctgctctt tctggtcggt tgcgtcttcg cctatttcgt ggtgctgccg
     5101 gccgcgatca acttcctgct cagcttcaac ggcgaacagt tcgcgatcca gttgagagcc
     5161 cgggagtact actcgttctt ctccatgacc ctgctcgccg gtggggtcgt cttcgagttg
     5221 ccgatcgtga ttctcgccct ggtcaggctg aggatcctga ccgtcgacca gctccggcag
     5281 aaccgtcgtt acgcctatct gatctgcgcg gtcatcgccg ccgccctgcc tggagtcgac
     5341 ccgatttcga tgctgatcga gacggtcccg ctactggttc tctacgagtt gagtatcttg
     5401 ctcgctcggg ggatcggtca gcccaaggaa agcgccgagt cccagattcc ttcaattcag
     5461 gagaactagc caagtggcca acgaacccga acacaagatg cttttcgacg tgcgcggtcg
//
LOCUS       DAOQKN010000166         5498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_7860_length_5498_cov_5.303142, whole genome shotgun sequence.
ACCESSION   DAOQKN010000166 DAOQKN010000000
VERSION     DAOQKN010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5498
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7860_length_5498_cov_5.303142"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..358
                     /locus_tag="PLJ64_10105"
     CDS             104..358
                     /locus_tag="PLJ64_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93877.1"
                     /translation="MPTTKPRYTVTDTGDLENLLDEAQGRWPDVKDRKDLLILLARTG
                     MAKVRADAENRRRVVDETSGALTGVYREDELSRLREDWPE"
     gene            355..741
                     /locus_tag="PLJ64_10110"
     CDS             355..741
                     /locus_tag="PLJ64_10110"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNC93878.1"
                     /translation="MIVLDASLLIAHLDAGDAHHEEAEELLAGTGSARLGASTITLAE
                     ILVAPARQGKLEPAWAAVKKLGVEEIPVMADSAPRLAELRAKTGLKMPDCCVLEAAGR
                     NNAEVATFDKSLANAAKGIGLTRSTG"
     gene            793..1614
                     /locus_tag="PLJ64_10115"
     CDS             793..1614
                     /locus_tag="PLJ64_10115"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNC93879.1"
                     /translation="MAEPIPDLPSEKQRDQLVTLLRPLLHVTQPKLYGAENVAKAGSL
                     LVGNHTLYAFLDLPFMMADLWSKHQVTVRGLGDNAHYNFPIWRDLLQVGGMVRGTREN
                     VRSLMDAGENVLVFPGGAREVNKRKGEKYQLIWKERLGFAKLAIEYGYPIIPFAAVGA
                     EDAYDIVLDETNPVYARFTGLVKKVTGWPMQPLARGIGPTLIPRPERLYFWFGEPITT
                     SGYAGDNGDSNARQVRDETKAAVEEGIAFLLREQGEDPQRDLSSRLLKRDSAKPV"
     gene            complement(1616..1939)
                     /locus_tag="PLJ64_10120"
     CDS             complement(1616..1939)
                     /locus_tag="PLJ64_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93880.1"
                     /translation="MKPAIYILSDSYVTFTDLAEAVAALEPDAKLPPVMPLTLARAIA
                     AGGEMVAKLTRKPPLIPRGGLHFLSSHAAPDSAKAVEELNWQITPLNEGLEKSLDFFR
                     RKGWL"
     gene            1958..2263
                     /locus_tag="PLJ64_10125"
     CDS             1958..2263
                     /locus_tag="PLJ64_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93881.1"
                     /translation="MAQISLRISDELAEDIKADAKARKLSVNGYITHILRSASDPEFG
                     GSEGERVRERLRRAGLLEEWLDEPEVERPDPKEIARIRAKAGKGTSLSDLVIEERRH"
     gene            2260..2697
                     /locus_tag="PLJ64_10130"
     CDS             2260..2697
                     /locus_tag="PLJ64_10130"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNC93882.1"
                     /translation="MIFADSSAIAKLYVDEAGSSHMRKFAVLLVSALCRVETTSAIRR
                     KARSGEINESEAEQLVRDFRADLRSKRTDGPELIALPVSTKLLNSAARLVTIHDLRSL
                     DAVQLATALAAREVDPQCDAFACYDERLARVAALHGFTIHSPS"
     gene            2758..3066
                     /locus_tag="PLJ64_10135"
     CDS             2758..3066
                     /locus_tag="PLJ64_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93883.1"
                     /translation="MPTKLRRIAVTEDVDLASALRSASQALPGLSDAALVKQLAIIGA
                     RSLSTQPPNDHLEHILRIPGAKLPSRPLGSLLKEIADLPKVSSAEVDRILEEERAERL
                     "
     gene            3066..3461
                     /locus_tag="PLJ64_10140"
     CDS             3066..3461
                     /locus_tag="PLJ64_10140"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNC93884.1"
                     /translation="MDAPAYFDTSALTKLILDEEESPRISEFMNTREVSLVSSQLSEV
                     ELLRAVSRSQPGSRPKARELLDRMVLLPITSSILERAAVLDPPSLRSLDAIHLATAVE
                     IKTHLAGVMTYDNRMRKAAEGLQLPVVVA"
     gene            complement(3491..3940)
                     /locus_tag="PLJ64_10145"
     CDS             complement(3491..3940)
                     /locus_tag="PLJ64_10145"
                     /inference="COORDINATES: protein motif:HMM:NF016001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HNC93885.1"
                     /translation="MDLQLRIADKTWPVLTKLMGGHARVYRLTRGRIGHHIPGVPPMC
                     LIDHVGAKSGKKRTSPLLYIEDGENIVIVASKGGYEKSPAWFYNLKANPDTTVQIGSE
                     VRPVRARVADEAERKRLWPKAVEVYPSYQQYQDRTNRKIPIVVLEPR"
     gene            4109..4798
                     /locus_tag="PLJ64_10150"
     CDS             4109..4798
                     /locus_tag="PLJ64_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93886.1"
                     /translation="MNAVRNQSLAIFFGLLFLLALGGQSIAGYQSYNDDEKARAHLAR
                     EAPETIDYPSYLTSPEFSRDVMENWQSEYLQFLLFILATIWLVQRGSPESKRPDESGT
                     ESDRDQKIGPYADPTSPLPARVGGLVTALYSNSLLILMGSIFFAAWFAQSLTGWPAYN
                     EEQVAYGLPTVSWLSYLGTADFWSRTLQNWQSEFLAVGSMAVFAIYLRQRGSPESKQV
                     GESHDSTGIEG"
     gene            4823..>5498
                     /locus_tag="PLJ64_10155"
     CDS             4823..>5498
                     /locus_tag="PLJ64_10155"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNC93887.1"
                     /translation="MFGNGAEGPRKSDGQMRSPIRVAVVGHVEWAEFAIVDHVPVAGE
                     IVQATETWEEPAGGGAVAAVQLAKLNGSCLFLTAVSDDPLGSQVKPELERHGLEVCAA
                     VQATKQRRAFVHLDSRGERTITTTGERLTPGAGDDLPWARLAGFDAVYVTAADAEGFR
                     LSRAARKLVATIRAARGLLESGVTVDVVVASRNDPGEKFDLAQFTNPPGCVVRTDGSK
                     GGTIAHS"
BASE COUNT         1106 a         1640 c         1736 g         1016 t
ORIGIN      
        1 atcgaagtcg acgaagaact cttcacgccc tcagcgggct aaggcttcaa gagccagact
       61 tagagggtgc gacacccata tagtttggtg ctacactctg gcgatgccaa ccacgaagcc
      121 ccgatacacg gtgactgaca cgggggacct cgagaacctg ctcgatgagg cacagggtcg
      181 ttggccggac gtgaaggacc ggaaggacct cctgatcctg ctggcccgga ccggcatggc
      241 gaaggtcagg gctgacgctg aaaaccgtcg gcgggtggtg gatgaaacca gcggcgccct
      301 gaccggtgtg taccgcgagg acgagctctc cagactccgt gaagactggc cggaatgatc
      361 gtccttgacg ccagcctcct gatcgcgcat ctggacgccg gcgacgcgca tcacgaggaa
      421 gcggaagagc ttctggcggg caccggaagt gccaggctcg gcgccagcac tatcaccctg
      481 gccgagatcc tggttgcgcc cgccaggcag gggaaactcg aaccggcctg ggccgcggtc
      541 aagaagctcg gggtcgagga aatccctgtg atggccgact cggctccgag gctggctgag
      601 ctccgagcga agaccggcct caagatgccc gactgctgtg tgcttgaagc ggcaggacgg
      661 aacaacgccg aagtggccac cttcgacaag tcactcgcaa atgcggcgaa agggatcggc
      721 ctgacgcgat caactggctg aagctcctcc gagcggcctg cgagagatcc cgtgaagtag
      781 gagaatcgtc gtatggcgga gccgatcccc gacttgccat cggaaaagca gcgggaccag
      841 ctggtcacat tgcttcggcc tctgttgcat gtcacccagc cgaagcttta tggggctgag
      901 aatgtggcga aagccgggtc actcctggtt ggcaatcaca ccctttacgc attcctcgac
      961 cttccgttca tgatggccga tctctggtcg aagcatcagg tgactgtccg cgggctcggt
     1021 gacaacgccc actacaactt cccgatctgg cgagatctcc ttcaggtcgg gggcatggtg
     1081 aggggtactc gcgagaacgt ccgctcactc atggacgccg gcgagaacgt cctggtgttt
     1141 ccgggagggg ccagggaggt caacaaacgc aagggagaga agtaccagct gatctggaag
     1201 gagcggctcg gattcgcgaa gctcgcaatc gagtacggat acccgatcat cccgttcgcg
     1261 gcagtgggcg ccgaggacgc ctacgacata gtcctggacg aaaccaaccc cgtctacgcc
     1321 cggttcacgg gattggtcaa gaaggtcacc ggctggccga tgcaaccgct cgcgcgtgga
     1381 atcgggccaa ccctgatccc gcgcccggaa cgtctctact tctggttcgg cgaaccgatc
     1441 acaacatccg gctatgccgg cgacaacggc gactcgaatg ctcgccaggt tcgagatgaa
     1501 acgaaagctg ccgtcgaaga aggaatcgcg tttttgctga gagagcaagg cgaggatccg
     1561 caacgggatc tcagttcccg gctcctgaaa agggactcag cgaagccggt ctaagctaaa
     1621 gccaaccctt cctccgaaag aaatccaggc tcttctcgag gccttcgttc aacggagtga
     1681 tttgccaatt gagttcttcg acggccttcg ctgaatcggg agccgcgtgc gaactcagga
     1741 aatggaggcc accacgtgga atgagcgggg gcttgcgggt cagtttggcc accatttcgc
     1801 cgccggcggc gatggcacgg gccaatgtga gaggcatgac cggaggcaat tttgcgtcgg
     1861 gctcgagtgc ggcaaccgct tctgcaagat cggtgaatgt gacgtagctg tcggagagaa
     1921 tgtaaatagc gggcttcacg agtgctagca tgaaagcatg gctcagatat cgctgcgaat
     1981 ctccgatgaa ctggccgaag acatcaaggc tgacgcaaaa gcacggaaac ttagcgtcaa
     2041 cggctacatc acgcacatcc tgcgatctgc gagcgatccc gaattcggcg gatcggaagg
     2101 tgagcgtgtt cgtgagcggc tccgacgagc gggattgctt gaggaatggc tcgacgaacc
     2161 agaggtggaa cgtcccgatc cgaaagaaat tgcgcgtatt cgggcaaaag ccggcaaagg
     2221 aacatcactc agtgacctgg tgatcgaaga gcgacgccat tgatcttcgc tgactcatcg
     2281 gcaatcgcga agctctacgt cgatgaggca ggttcctctc acatgcggaa gttcgccgtg
     2341 cttctggtgt cagcgctgtg ccgggtagag acaacttctg cgatccggcg aaaggcgcgt
     2401 agcggcgaga tcaacgagtc cgaagcggaa caactggtcc gggacttccg ggcagatctg
     2461 cgatcgaagc ggaccgacgg acccgaactc attgctttgc ccgtatccac gaagctgctg
     2521 aattccgcgg caaggctcgt gacaatccac gacctcaggt cactcgacgc ggtccagttg
     2581 gcgaccgcgt tggctgccag ggaggtggat ccccaatgtg acgcgttcgc ctgctacgac
     2641 gaacggctgg ccagggttgc cgctctacac ggcttcacca tccattctcc aagttgacat
     2701 aagacacatt atcgagcatt gaagaaagtg tgccaccgac agtgctacac tgcgctaatg
     2761 cccaccaaac tgaggagaat cgcggtaacc gaggacgttg atctcgcttc tgccctgcga
     2821 tcggcaagcc aggcgctgcc gggactcagt gacgcggccc tggtcaagca actggcgatc
     2881 attggagcaa ggagtctctc cacgcagccc ccgaacgacc atcttgagca catcctcagg
     2941 attcccgggg cgaaattgcc ttctcggccg cttggttcgc tcctgaagga aatagcggat
     3001 ctgcccaagg taagttccgc cgaggttgat cggatcctcg aggaagaacg ggcagagcgg
     3061 ctttgatgga cgcccccgcc tatttcgaca cctcggcgtt gacgaagctg attctcgacg
     3121 aggaagagag tcctcggatc agcgaattta tgaacactcg cgaagtgagc ctcgtctcga
     3181 gtcaattgag cgaagttgag ctcttgcgtg cggtttcacg gtcgcagccg ggcagccggc
     3241 cgaaagccag ggaattgctg gaccgcatgg ttcttctgcc gattactagc tcgatcctgg
     3301 aaagagccgc ggttctggat ccaccttctc tgagaagcct ggacgcgatc cacctggcga
     3361 ccgccgtcga gatcaagact catctggcag gcgtaatgac ttatgacaac cggatgcgta
     3421 aggcggctga aggcctgcaa ctccctgtcg tcgtcgcctg actggcgatc agaaagggaa
     3481 ctctcggtga ttagcgtggc tcgaggacga cgatcgggat cttccggttg gtgcggtcct
     3541 ggtactgctg gtatgacggg tagacctcga ccgctttcgg ccagaggcgt ttgcgttcgg
     3601 cttcgtcggc gacgcgggcc cggaccggac ggacttcgct gccgatctgg accgtagtgt
     3661 ccgggttggc cttgaggttg tagaaccagg ccgggctctt ctcgtagccg cccttcgagg
     3721 cgacgatcac gatgttctcg ccatcttcga tgtagagcag cgggctcgtg cgtttctttc
     3781 cgctcttcgc gccgacgtga tcgatcaggc acatcggagg cacaccggga atgtgatgac
     3841 cgattcgtcc cctggtgagg cggtaaacgc gagcgtgacc gcccatcagc ttcgtgagca
     3901 ctggccacgt cttgtcggca attcggagct ggaggtccat tgcctcaacc tatcgtggcc
     3961 cgacgacacg ggctcccgct tctcggtcct gccggtacga aaagggcgcc ggcgggtaca
     4021 cggcgggtat gagggtcgtc ttcttcgtgt atctgatcgg ggtgattgcg ggccttgcgt
     4081 ttgcgatctc ggtgggagca aacagcgcat gaacgcggtc cggaaccaga gcctcgcgat
     4141 cttcttcggc ctgctctttc tgcttgcgct cggaggccag tcgatcgccg gctaccagag
     4201 ctacaacgat gacgagaagg cgcgtgccca cctggcgcgc gaggctcccg aaaccatcga
     4261 ctaccccagc tacctgacct caccggaatt ctcgcgagac gtgatggaaa actggcagtc
     4321 ggaatatctg cagttcctgc tcttcatcct cgcaacgatc tggctggtcc agcggggctc
     4381 gcctgaatcg aagcgacccg acgaaagcgg caccgagagt gaccgcgacc agaaaatcgg
     4441 accgtacgca gacccgacct ctcccctccc cgcccgggtc ggcggtctcg tcaccgctct
     4501 ctactcgaac tcgctactga tcctgatggg atcgatcttt ttcgcggcct ggttcgccca
     4561 gtcacttacc ggctggcctg cctacaacga agaacaggtc gcctacggcc tgcccaccgt
     4621 cagctggttg agctacctcg gaaccgctga cttctggagc cgcacgctcc agaactggca
     4681 atcggaattc ctggcggtcg gttcgatggc cgtcttcgcg atctacctga ggcagcgagg
     4741 ctcgcctgaa tccaagcagg ttggcgagag ccacgattcg accgggatcg agggttgatg
     4801 cccgatcacc tagttttcgg ggatgttcgg aaacggagct gagggtccgc gcaagagcga
     4861 cggacagatg cgttccccga tcagggttgc tgtcgtcggc cacgttgaat gggccgagtt
     4921 tgcgatcgtc gaccatgtgc cggtggccgg ggagatcgtc caggccaccg agacctggga
     4981 agagccggcc ggaggcggtg ccgtcgccgc ggttcaactg gcgaaactca acggctcctg
     5041 cctctttttg accgcggtca gcgatgaccc gctcggcagc caggtgaagc ccgagcttga
     5101 gcgtcacggg ctcgaggtct gcgccgccgt tcaggcaacg aagcagcgtc gcgccttcgt
     5161 tcatctcgat tcccgaggcg agcggacgat cactacgacc ggggaacggc tgacgccggg
     5221 agcgggcgac gatctgcctt gggcacgcct cgcaggcttc gacgcggtgt acgtgaccgc
     5281 ggccgacgcc gagggcttcc gcctgagccg ggccgcgagg aagctcgtcg cgaccatccg
     5341 ggccgcccgc ggcctgcttg agtccggggt cacggtcgac gtcgtggtcg cgagccggaa
     5401 cgaccccggt gagaagttcg atctcgccca gttcacgaat ccgccgggat gtgtggtgcg
     5461 aaccgacggg tcgaaaggcg gcaccatcgc ccactccg
//
LOCUS       DAOQKN010000167         5495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_7867_length_5495_cov_5.853493, whole genome shotgun sequence.
ACCESSION   DAOQKN010000167 DAOQKN010000000
VERSION     DAOQKN010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5495
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7867_length_5495_cov_5.853493"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="PLJ64_10160"
     CDS             complement(<1..253)
                     /locus_tag="PLJ64_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93888.1"
                     /translation="MGERRRLILAFVLGLVIATAGTATAAKLITGKQIKDGTITSKDL
                     SRALRKQISRSGKAGPTGPAGVTGPAGVTGPTGAVDASGF"
     gene            285..1211
                     /locus_tag="PLJ64_10165"
     CDS             285..1211
                     /locus_tag="PLJ64_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93889.1"
                     /translation="MSFSGSARRFLAVAGVAVFTIAVGSASATVPGKNGLLLVSGYTD
                     GGINGSTSRIFTETLTGKSKELLGALDQSYSDPAVSPNGKQIVYSVYPGYRMWLGSFA
                     KPKQAKAITPEESDVLNLETVFAPDGKSIYFSKKYYLDSGVFWHLKKYTFKTKKTTSY
                     KVDTKLDWGFSDVSPNGRYLAYNRGGDEDASKIRLLDTRTGKSHTVNTKGAALGPAFS
                     PDGKSMVYTAQYGDAWELVQSRLDGKNARRLTRTGAINFDPHYSPDGKMIAFTQGVEG
                     NKKIGILTLKTGKIRYLVATGDYAEVEQWLRK"
     gene            complement(1340..2680)
                     /locus_tag="PLJ64_10170"
     CDS             complement(1340..2680)
                     /locus_tag="PLJ64_10170"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HNC93890.1"
                     /translation="MKHSLPLLAAVLSLALAAPSAAASPDPLPNPDRHFATPFMHRGE
                     KGASGPADASASAGRACQVMRMLARRGNGAVGLRVFNLRSRKNVCALNSGARRSLASN
                     NKLFTISTALDRLGQDQRLQTRVMAGGKTSRNGVLDGNLYLKGGGDPSLGTARFVNRW
                     FGGHGSEIEKLARKVRQAGIRKVTGRLFFDDSVFDQVRGVADSGYATSPWIGPLSGIA
                     FNEGYTSPSLSRFSNDPARLAARTFVRAMRARGVKIRPRFSPRDTPVKLLKRVVAREV
                     SPDMGWMARATLLDSNNFFAETLLKVLGASFHGLGTTANGVKVVRRTMASLKVDVWPI
                     DGSGLTYGNRSTAHDVVKLLFRARSEPWGKALVAGMPTAGVDGTLEDRMRGSAAQGNC
                     HAKTGTLTGVSALSGYCFNSSGRRFAFSILMNDVTNLDTAHRAQDRIAALIARL"
     gene            2752..4011
                     /locus_tag="PLJ64_10175"
     CDS             2752..4011
                     /locus_tag="PLJ64_10175"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNC93891.1"
                     /translation="MGKVVNIEESQKRRQSAKRQKRSRTALVLGGGGFTGGVYEIGTL
                     RALDLLAVNSTVNDFDMYIGTSAGSFIGSMLAAGVTPEEMMRVLTDEKGALLERISQD
                     TVLRPNFSEYAERIVTLPLHVAGMVKSMVPRFRDLSVIDIAYGLAEHLPSGMYSNAGI
                     GAYVREALATKGIVDSFDALEHELYLTGTDLDSGERVVFGDKEGEWSGIPVSKAVECS
                     TALPLVYEPVEVNGRTLVDGGIYSTTNVDVAVERGAEFIVVVNPLVPYVNDRNVGLPT
                     VSGSRVRRIADMGLPAIANQAFRLLLHQRLAIAVESWKERYPGVDILLFEPDPDDELM
                     FGTSIMDYSRRLDIATHGFETVTAHLAEDYAYYTEVSEKHGIEISATRLRRVVRKVKE
                     QESSEASVWRRIFEQTTGALLRQTGTS"
     gene            4351..4977
                     /locus_tag="PLJ64_10180"
     CDS             4351..4977
                     /locus_tag="PLJ64_10180"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNC93892.1"
                     /translation="MTRVGQAFDNPVTGERVVVLTDPFEHPDRVLAAHLTVAPGGRVA
                     MAHIHPAVRERFHVLGGQVGFLVGDEEVTLGPGESAEVPAGVVHDWWQIGEEEASVVV
                     EATPGDRLSEFITSIFGLCRDGKVNEDGVPRLLQIMVMVNEYSDEMILSSPPPAIQKT
                     IAAMLAPIGRLRGLKPSYPEYTTSTETVEPAPEALALLDEDGRLKGFA"
     gene            complement(4978..>5495)
                     /locus_tag="PLJ64_10185"
     CDS             complement(4978..>5495)
                     /locus_tag="PLJ64_10185"
                     /inference="COORDINATES: protein motif:HMM:NF024591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNC93893.1"
                     /translation="KNPEATAETITDGWLHTGDIASIDEDGYVSIIGRKKEIIITAGG
                     KNITPVNIEQEVKRHPLVSQCVVIGDRRPYLIALITLDQEALTRFAAEQGMLDEPGAM
                     VGSPLVRETLEQHIEEVNRNFARVEQIKRFHILPHDLSQEGGELTPTLKIKRPVVATK
                     YEKEIEDLYAS"
BASE COUNT          966 a         1740 c         1809 g          980 t
ORIGIN      
        1 agaaaccgga ggcgtcgacc gccccggtgg gcccggttac acccgccggt ccggtcacgc
       61 cggccggacc ggtcgggccg gccttgccgg atctgctgat ctgcttgcga agcgccctgg
      121 acagatcctt cgaggtgatc gtgccgtcct tgatctgctt tccggtgatc agctttgccg
      181 cagtcgccgt cccggcggtg gcgataacca ggccgaggac aaaagcgagt atcagccttc
      241 tccgttctcc catgtgtcat tcataaccga ttaggctggg cggcatgagt ttttcaggga
      301 gtgccagaag gtttctggcc gtggcggggg tcgcggtctt cacgatcgcg gttggcagcg
      361 cctcggcgac ggtgccgggc aagaacgggc tgctgctcgt ttccggctac accgatggtg
      421 gcatcaacgg gagcacgagc cgcatcttca ccgagaccct gaccggaaag tcgaaggaac
      481 tgcttggcgc cctcgaccag tcgtattccg atccggcggt ctcaccgaac ggcaagcaga
      541 tcgtctactc ggtctatccc ggctaccgga tgtggctcgg ctccttcgcc aagccgaagc
      601 aggcgaaggc gatcacccca gaggaaagtg acgtgctgaa cctcgaaacc gtattcgcgc
      661 cggacggcaa gtcgatctac ttctcgaaga agtactacct cgattcgggg gtcttctggc
      721 acctgaagaa gtacaccttc aagacgaaga agacgacgtc ctacaaagtc gacaccaaac
      781 tggactgggg cttctccgac gtttcgccca atggccgtta cctcgcctac aaccggggcg
      841 gcgacgagga cgcctcgaag atcaggctgc tggacacgcg caccggcaag agccacacgg
      901 tcaacacgaa aggcgcggcg ctggggccgg cgttctcccc cgatggcaag tcgatggtct
      961 acaccgcgca gtacggggac gcctgggagc tggtccagtc acgccttgac ggcaagaacg
     1021 ccaggcgact gacccggacc ggggcgatca acttcgatcc tcactattcg cccgacggca
     1081 agatgatcgc cttcacccag ggggtcgaag gaaacaagaa gatcggcatc ctcaccctca
     1141 aaaccggcaa gatccggtat ctcgtggcga ccggcgacta cgccgaagtc gagcaatggc
     1201 tcaggaaata aggagtaggg cgggatccaa cccgccctcc ccataaaccc accggtagag
     1261 ccccgcccaa ccattgttta cttcgaatgg gtggggcggg ttcagcatgg agggccggcg
     1321 aagaccggat ggcatgcagt taaaggcggg cgatcagggc ggcgatgcgg tcctgtgctc
     1381 ggtgggcggt gtccaggttg gtcacgtcgt tcatcaggat cgagaaggcg aatcgccggc
     1441 cggagctgtt gaagcagtat ccggagaggg ccgagacgcc ggtcagggtg ccggtcttcg
     1501 catggcagtt gccctgggca gcgctgccgc gcatccggtc ctcgagggtg ccgtccaccc
     1561 cggcggtcgg catgccggcg acgagggcct tgccccaggg ttcgctgcgg gcgcggaaga
     1621 gcagcttgac cacgtcgtga gcggtggacc ggttcccgta ggtgaggccc gagccgtcga
     1681 tcggccagac atccaccttc agcgaagcca tggtccggcg caccaccttg accccgttgg
     1741 cggtcgttcc caggccgtgg aaggaggccc cgagcacctt gagcagggtt tccgcgaaga
     1801 agttgttcga gtccagcagg gtggcccggg ccatccagcc catgtcgggg gaaacctcgc
     1861 gggcgactac ccgcttgagc agcttcaccg gtgtgtcccg gggtgagaat cgcgggcgga
     1921 tcttcactcc gcgggcccgc atcgcccgaa cgaaggtccg ggccgccagc ctggccgggt
     1981 cgttgctgaa ccgggaaagc gaagggctcg tgtagccctc gttgaaggcg attccggaga
     2041 gcggcccgat ccaggggctg gtcgcataac cggaatcggc gacgccacgg acctggtcga
     2101 agaccgaatc gtcgaagaag agccggccgg tgaccttgcg gatccccgcc tggcggacct
     2161 tccgggccag cttctcgatt tccgatccgt gaccaccgaa ccagcggttc acgaacctgg
     2221 cggttccgag gctggggtcg ccgccgccct tcaggtagag gttgccgtcg agaaccccgt
     2281 tccggcttgt cttgccgccg gccatcaccc gggtttgaag ccgctgatcc tggccgagcc
     2341 ggtcgagggc ggtggagatc gtgaagagct tgttgttcga ggccagcgaa cggcgggccc
     2401 ccgagttcag ggcgcagacg ttcttcctgc tgcgaaggtt gaacacccgc aggccgaccg
     2461 caccattgcc tcgcctggcc agcattctca tcacctggca ggcccggccc gcgctggcgc
     2521 tcgcatcggc cgggccggac gcgccctttt caccccggtg catgaacggt gtggcgaaat
     2581 gccggtccgg attcggcagc gggtcggggg aagcggccgc ggaaggagct gcgagggcca
     2641 gggaaagcac cgcagcgagg agcggaagag agtgtttcat cggatattgc aacaaacttg
     2701 gggccgaagg tagcggaagg tgacaccgat tgctgtcata attggaccct catgggcaag
     2761 gtcgtaaaca tcgaggaatc ccagaaacgg cgccagagcg ccaaaaggca gaagcgttcc
     2821 cgcactgcgt tggtcctcgg tggcggtggt ttcaccggcg gcgtatacga gatcggaacc
     2881 ctccgggcgc ttgatctcct tgccgttaat tcgacggtca atgacttcga catgtacatc
     2941 ggcacaagcg ccggctcgtt catcggcagc atgctcgcgg ccggggtcac cccggaggaa
     3001 atgatgcggg tcctgaccga tgaaaagggc gccctgctcg aacggatcag ccaggacacc
     3061 gtccttcgcc cgaatttcag cgaatacgcg gagcggatcg tcaccctgcc gctgcatgtc
     3121 gccggcatgg tcaagagcat ggtcccgcgc ttccgtgacc tgtcggtgat cgacattgcc
     3181 tacggcctgg ccgagcacct gccttccggc atgtactcca acgcgggcat cggcgcctat
     3241 gtgcgcgagg cgctcgcgac caagggcatc gtcgactcct tcgacgcgct cgagcacgag
     3301 ctttacctga ccggcaccga cctcgattca ggtgaaaggg tcgtattcgg tgacaaggaa
     3361 ggcgaatggt ccggcatccc cgtttccaag gctgtcgaat gttcgaccgc cctgccgctc
     3421 gtctacgaac cggtcgaggt aaacggccgc accctggtcg acggtggcat ctactcgacc
     3481 accaacgtcg acgtggcagt cgagcgtggt gccgagttca tcgtcgtggt caatccgctg
     3541 gtgccctatg tgaacgaccg caacgtcggt ctgccgaccg tttccggcag ccgggtccgg
     3601 cggatcgctg acatgggcct gccggcgatc gccaaccagg ccttccgtct gctccttcac
     3661 cagcgtctgg cgatcgcggt cgaatcctgg aaggaacgct accccggggt tgacatcctg
     3721 ctcttcgagc ccgatccgga cgacgagctg atgttcggca cctcgatcat ggattactcg
     3781 cgccgtcttg acatcgccac ccacggattc gagaccgtga ccgctcacct cgccgaggat
     3841 tacgcctact acaccgaggt cagcgagaag cacggcatcg agatctccgc cacccgcctg
     3901 cgccgcgtgg ttcgcaaggt caaggagcag gaaagctccg aggcttcggt ctggcgccgc
     3961 atcttcgagc agaccaccgg cgctctcctc cgccagaccg gcacttccta gcccgggtct
     4021 cctggatcac gccgcgggat cgggcaggca tcaccgggac aacagtcggg cggcattcag
     4081 ccgccctctg tttgagcgtc ccggagacgc ctgcccgacc ccacggctac tcgaacccgg
     4141 gcgatgcctc cccggcagag cacggctgaa ggccctggca tccacccgca ggagcgcctt
     4201 gggcacgacc cgctgaggca agtggtgaat tcttttcgcg agatgcggtt tgtcggcgta
     4261 tagcgccgat gaatgcgatc tcgagctgaa cttcacccga acgggcagcg tggctctcgc
     4321 cgtggggtcc gttgcggata ggttctggac atgactcggg ttggccaggc gttcgataat
     4381 ccggttaccg gggaacgggt ggtagtgctc accgatccct ttgagcatcc cgaccgggtt
     4441 ctggccgcgc atctgaccgt ggcgccgggt ggccgggtcg cgatggcgca catccacccg
     4501 gccgttcgcg agcgcttcca cgtgctgggt gggcaggtcg ggttcctggt cggggacgaa
     4561 gaagtgaccc tcggtccggg cgagtcggcc gaggtgccgg ccggcgtggt tcacgactgg
     4621 tggcagatcg gggaagagga ggcgagcgtg gtggtcgagg cgacgccggg cgatcgcctc
     4681 tccgaattca tcaccagcat cttcgggctc tgccgcgacg gcaaggtgaa cgaggatggc
     4741 gtgccccgtc tccttcagat catggtcatg gtcaacgagt acagcgacga gatgattctg
     4801 tcctcaccgc cgccggcgat ccagaagacg atcgcggcga tgctggcccc gatcggccgc
     4861 ctgaggggcc tcaaaccctc ctacccggag tacacgacct cgaccgaaac cgtggaacct
     4921 gcgccggagg ctctggcgct gctcgacgag gacggtcgcc tgaaagggtt cgcctagtca
     4981 gctggcgtag aggtcttcga tttccttctc gtacttggtt gccacgaccg ggcgcttgat
     5041 tttcagggtc ggcgtcagct cgccgccttc ctggctcagg tcatggggca ggatgtggaa
     5101 gcgcttgatc tgctcgaccc gggcgaagtt cctgttgact tcctcgatgt gctgctcgag
     5161 cgtctcgcgg accagtgggc tgccgaccat cgcacccggc tcgtccagca tgccctgctc
     5221 ggcggcgaag cgggtcagcg cttcctggtc gagggtgatc agggcgatca ggtacggccg
     5281 ccggtcaccg atcaccacgc actgggaaac gagcggatgc cgcttgactt cctgctcgat
     5341 gttgaccggg gtgatgttct tgccgccggc ggtgatgatg atctccttct tgcggccgat
     5401 gatcgagacg tagccgtcct cgtcgattga ggcgatgtca ccggtgtgga gccagccgtc
     5461 ggtgatcgtc tcggccgtcg cctcggggtt cttgt
//
LOCUS       DAOQKN010000168         5407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_8085_length_5407_cov_5.471599, whole genome shotgun sequence.
ACCESSION   DAOQKN010000168 DAOQKN010000000
VERSION     DAOQKN010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5407
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8085_length_5407_cov_5.471599"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="PLJ64_10190"
     CDS             complement(<1..731)
                     /locus_tag="PLJ64_10190"
                     /inference="COORDINATES: protein motif:HMM:NF019138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HNC93894.1"
                     /translation="MSDLKSKSLAELHELAAKAGIEKFRLLRKDELAEKLGDADGAAG
                     AEDATAGDASGDSGSSESDRGSRRRGGRDRGRGRGDREPRSDRERRGGGDRDRKNGRG
                     DREGGDGREDSEGREEEAEAELSEITGVLDVLPRGSGIIRSDDGPSAGIYVSPAQIRR
                     CELRSGDEVSGPVRPARRGERRPSLVRVEKVNGEPPVDERSPRFESLTPVAPHRHMPL
                     SGDADDILARSVDLLAPLAFGQRVLV"
     gene            complement(733..1419)
                     /gene="fsa"
                     /locus_tag="PLJ64_10195"
     CDS             complement(733..1419)
                     /gene="fsa"
                     /locus_tag="PLJ64_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HNC93895.1"
                     /translation="MELFIDTGAVSEVEEIAAWGVLSGATTNPSLLAKEDGEPGDIIR
                     KIANLVNGPTSAEVVSPDAEGMIAEGQALRKLHEHVTVKVPFGPEGLKATRALTDDGA
                     MVNMTLVFSPNQALLAASAGATFVSCFMGRLDDISVDSAKVLEEICDVLRARDLDSKV
                     LAASIRHPEHMVVAASLGCDIATVPAKVFHQMLNHPLTTEGAAKFEEDWKSRPEFGEW
                     LGGLVAADKG"
     gene            complement(1470..3146)
                     /locus_tag="PLJ64_10200"
     CDS             complement(1470..3146)
                     /locus_tag="PLJ64_10200"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HNC93896.1"
                     /translation="MVGRSQSLEAIAGSHFEVVVIGGGITGAGVALDAASRGYSVALL
                     EGRDYAIGTSSRSSKMVHGGLRYLENFDVGLVREALLERQLLVELAPHLVYPTPFLVP
                     SFGEEKPDRKVGLGLGAYDVLAGATSGRNRRERRWRRRPAEAEESWSPDRHRTIDREE
                     LLELVPALETRDPESAYLFYDCQTDDARLVLTVLGEAERFGAVCLNGANVTRVLEKSG
                     KARQVEFTEIASGETVVVEADNIVNATGVWADQIRPGEIEREEDIPKIAPSRGAHLLF
                     SADDVDMGRAACIVPAGEGRRIFALPWYGRTLVGTTDVDHDGGIEEVAATEDDIEYLM
                     NALNEFMDLSLTDSDIVGAFAGVRPLISTGDPRKSVDISRQAELYETSSGMLTITGGK
                     LTTWRRMAKQTVDRIVERAGREARCQTAEIPLGMEIDPAELSAPEGVSKESLAQLAFR
                     YGHAARGVIRIAESDPEMARPIVDGMPDLMAEARIAIEFEQARSLADVLLRRTRLGLT
                     AASSLATADSVEPLAAVMAHGLGWGDIEKKRQIDEWLGTVKAEGLNPAGS"
     gene            complement(3140..5152)
                     /locus_tag="PLJ64_10205"
     CDS             complement(3140..5152)
                     /locus_tag="PLJ64_10205"
                     /inference="COORDINATES: protein motif:HMM:NF018816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HNC93897.1"
                     /translation="MSWQLVSVVVLLFTLGAGMLWYERSRPPSQIVALVAVLAALAVA
                     GRIVLAPIPNVVATTDIVIIAGFVLGPAPGFAVGALGGLVSNFWLGQGVWTPWQMVAW
                     GMCGVAGGVLFHLARGRVNRWTLAAFCGLAGVVFGAWMNLETMVAFGGEISLDRYLAL
                     EVRAIPFDIAHMTGNIIFALAAGPAMIAALTRFRDRFQWRQVPTGEAAPPDPGPPRDR
                     PAQPAGAAGVSLILLAALLIAFAGGGPQRAEAAYTGEAKSAAAWLKTQQNTDGGFSSI
                     PGSTSSVGITARAMFALAAAGTNPLDVKTNATPYGYLVNHRDDINDASDIALTILAMK
                     TVGQDPRDFKGRNLIKALRARRGNGSFGNDVNVTAFAVLAFRSVAATKDAQYSLDWLH
                     GAQNDNGGWGISKGSDSDSDSTGTALMAVTGDKVANRAIKFLDGIQKKSGGFGNSGNV
                     NAQSTGLVMQGLVAQDRSVNYLKKNGNSAADYLLSLQQDDGSIFYARDNDVTRVWVTA
                     DGTTALAGQPLPIGAPPRADNGNDANNGSGGTAPGDGSNTGGTSTGGTLPAGSLAGTG
                     NTGSASAGSGSGANGGNGGNQGSGNGNGSGSGSGTNGGDGSSSATTLPPTDTVAPVIP
                     EAPSAEVLAASEAGPQPSPLVATLVGLAVSGFLCGGTILLVRRLNW"
     gene            complement(5149..>5407)
                     /locus_tag="PLJ64_10210"
     CDS             complement(5149..>5407)
                     /locus_tag="PLJ64_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HNC93898.1"
                     /translation="GHLTELAGRLSGQGAAVVISTHDVEFAVSFADRVVLLGDGEVVA
                     DAEMPTVLSGGWYFASEAARVLDQPGLVSVEQGIEFLKRET"
BASE COUNT          840 a         1847 c         1822 g          898 t
ORIGIN      
        1 accagcaccc gctggccgaa ggccagcgga gcgagcaggt caaccgagcg ggcgaggatg
       61 tcatcggcgt cgccgctcaa cggcatgtgc cgatgcggag caacgggggt cagactctcg
      121 aagcgggggg agcgctcgtc caccggcggc tcgccgttga ccttctcgac ccggaccagt
      181 gaaggccggc gctcgccacg gcgggccggg cggaccggac cgctgacttc gtcgccggag
      241 cggagttcgc aacggcggat ctgggcaggc gagacgtaga tcccggccga gggaccatcg
      301 tcggagcgga tgatgccgct gccacgcggc agtacgtcga gcaccccggt gatttcgctg
      361 agttcagctt ccgcttcctc ttcgcggcct tcggaatcct cacggccgtc accgccctcc
      421 cggtcgccgc ggccattctt gcggtcacgg tccccgccgc cgcgccgttc acggtcactg
      481 cgaggctcgc ggtctccgcg gccacgaccg cggtcccggc caccccggcg acgcgatccg
      541 cggtcggatt cggagctgcc ggaatcgccg gaagcgtcac ccgcagtggc atcttcggcc
      601 ccggcagcgc catcggcgtc accgagcttc tcggccagct cgtccttgcg caggagccgg
      661 aatttctcga tacctgcctt cgcggcgagc tcgtggagct cggccagcga cttcgatttc
      721 agatcggaca tgtcagcctt tgtcggcagc gaccaggcca cccagccact cgccgaactc
      781 ggggcggctc ttccagtcct cttcgaactt ggcagcgccc tcggtggtga gcgggtggtt
      841 cagcatctgg tggaagacct tggccggcac ggtggcgatg tcacagccga gcgatgccgc
      901 gacgaccatg tgctcgggat gccggatcga ggcggccagc accttcgaat cgaggtcgcg
      961 ggcacgcagc acgtcgcaga tctcttccag caccttggcc gaatcaaccg agatgtcgtc
     1021 gagcctgccc atgaagcagg agacgaacgt tgcgccggcc gaggcggcca gcagggcctg
     1081 gttcggcgag aagacgaggg tcatgttgac catcgcgccg tcatcggtca gcgcccgcgt
     1141 ggccttgagg ccttcggggc cgaacgggac cttgaccgtc acgtgttcgt ggagtttgcg
     1201 cagagcctgg ccctcggcga tcatcccctc ggcgtcaggg gagacaactt cggcagaggt
     1261 gggtccgttg accaggttgg cgatcttgcg gatgatgtca ccgggttcac cgtcttcctt
     1321 ggcgagcagg gagggattgg tggtcgcgcc actgagcacg ccccaggcgg caatctcctc
     1381 gacctcggat acggcaccgg tgtcaatgaa aagttccatc gccccatggt attgagtcga
     1441 gcccgcggag cccggcccga tcagaagttt caggaaccgg ccgggttgag accttcggcc
     1501 ttgacggtgc caagccactc gtcgatctgg cgcttcttct cgatgtcacc ccagccaaga
     1561 ccgtgcgcca tcaccgcagc gaggggctcg accgagtcgg cggtcgcgag gctggaggcg
     1621 gcggtcagcc cgagcctggt tcgccgcaac agcacatcgg ccagcgagcg ggcctgctcg
     1681 aattcgattg cgatgcgggc ttcagccatc aggtccggca tcccgtcgac gatcggtcgg
     1741 gccatttcgg gatccgattc ggcgatgcgg atcactcccc tggcggcgtg gccgtaacgg
     1801 aaggcgagct gggccagcga ctccttgctg acgccttccg gggcgctcag ctcggccggg
     1861 tcgatctcca tcccgagcgg gatctcggcc gtctggcagc gcgcttcccg gcctgccctt
     1921 tcgacgatcc ggtccacggt ctgcttcgcc atccgccgcc aggtggtgag cttgccaccg
     1981 gtgatggtca gcatccccga tgaggtctcg tagagctcgg cctggcggga gatgtcgacg
     2041 gatttgcggg gatcacccgt gctgatcagg ggcctgaccc cggcgaaggc cccgacgatg
     2101 tcggagtcgg tcagggaaag gtccatgaac tcgttcagcg cgttcatcag gtactcgatg
     2161 tcgtcttcgg tggccgcgac ttcctcgatg ccgccgtcgt gatcgacgtc ggtggtcccg
     2221 accagggtcc ggccgtacca gggcaaggcg aagatcctgc ggccctcgcc ggccggcacg
     2281 atgcaggcgg cccggcccat gtctacgtca tcggcggaga aaagcaggtg ggcgccccgg
     2341 ctcggtgcga tcttcgggat gtcctcttcc cgttcgattt cgcccggccg gatctggtcg
     2401 gcccagaccc cggtcgcgtt gacgatgttg tcggcctcga cgaccacagt ttcgcccgat
     2461 gcaatttcgg tgaactcgac ctgccgggcc ttgccgctct tctccagcac ccgggtgacg
     2521 ttggcgccgt tcaggcagac cgcgccgaag cgttcggcct cgcccagcac ggtgaggacg
     2581 agccgcgcgt catcggtctg gcagtcatag aagaggtagg ccgattcagg gtcgcgcgtc
     2641 tccagggccg ggaccagttc gagcagctct tcccggtcga tcgtccggtg ccggtccggt
     2701 gaccaggatt cctccgcctc ggccggtcgc cggcgccagc gccgctcgcg gcggttgcgc
     2761 ccgctggtcg ccccggccag cacgtcgtag gccccgaggc ccagcccgac cttgcgatcc
     2821 ggcttctcct ccccgaagct cggcaccaga aacggggtcg gatagaccag atgcggagcc
     2881 agctcgacca ggagctggcg ctcgagcagc gcttcacgga ccagccccac gtcaaagttc
     2941 tcgaggtacc ggaggccgcc gtgaaccatt ttcgaggagc gggatgaggt tccgatcgcg
     3001 tagtcgcgac cttccagcaa ggcgaccgag tagccgcggg aggccgcgtc gagcgcgact
     3061 ccggccccgg tgatcccgcc gccgatcacg accacctcga aatgcgatcc ggcgatcgcc
     3121 tccagcgact ggcttcgacc taccaattga gccgccggac cagcaggatg gtgccgccgc
     3181 agaggaaacc gctgaccgca agcccgacca gggtcgcaac tagcggtgaa ggctgagggc
     3241 cggcctcgga tgcggccagc acttcggcgc tcggcgcttc cggaatgacc ggtgccacgg
     3301 tgtcggtcgg gggcagcgtg gtggcagaag atgatccgtc gccgccattg gtgccgctgc
     3361 cgctgccgga gccgttgccg ttcccggagc cctggttgcc gccgttgccg ccgtttgcgc
     3421 cgctgccgga accggcactg gcgctgcccg tgttgcccgt cccggcgagc gacccggccg
     3481 gcagggtccc gccggttgag gtgccgccgg tgttcgagcc gtccccggga gcggtgccgc
     3541 ccgagccgtt gttcgcatcg ttgccattgt cggcccgcgg cggcgccccg atcggcaagg
     3601 gctgcccggc cagagcggtc gtgccgtcgg cggtcaccca gaccctggtc acgtcgttgt
     3661 ccctggcgta gaaaatgctg ccgtcatcct gttgcagcga aagcaggtag tcggcggccg
     3721 agttgccgtt cttcttcagg tagttgacgc tgcggtcctg ggcgaccaga ccctgcatta
     3781 cgaggccggt cgactgtgcg ttgacgttgc cgctgttgcc gaaaccgccg gacttcttct
     3841 ggatcccgtc gaggaatttg atcgcccggt ttgccacctt gtcgccggtg accgccatca
     3901 gggcggtacc ggttgaatcg gaatcgctgt ccgagccttt ggaaatgccc cagcctccgt
     3961 tgtcgttttg cgccccgtga agccagtcga gtgaatactg cgcgtctttg gtggcggcga
     4021 ccgagcgaaa ggcgagcacc gcgaaagcgg tcacgttcac gtcgttgccg aaggaaccgt
     4081 tgccgcgcct ggccctcagc gccttgatca ggttgcgccc cttgaagtcc cgcgggtcct
     4141 ggccgaccgt cttcatcgcg aggatggtca gggcgatgtc gctggcgtca ttgatgtcgt
     4201 cgcggtgatt gaccagatag ccgtagggcg tggcgttggt cttcacgtcc agcgggttgg
     4261 tcccggccgc ggcgagagcg aacatcgccc gggcggtgat gccgaccgaa gaggtgctgc
     4321 ccgggatcga ggagaaaccg ccgtccgtgt tctgctgggt cttcagccag gccgcggcgc
     4381 tcttcgcttc gcccgtgtac gccgcctccg ccctctgcgg gcctccgccg gcaaaggcga
     4441 tgagcagcgc cgcgagcagg atcaggctca ccccggcggc acctgccggt tgcgcgggcc
     4501 ggtcccgggg cggcccggga tcaggcgggg ccgcttcccc ggtcgggacc tgacgccact
     4561 ggaaccggtc gcgaaagcgg gtcagggcgg cgatcatcgc cggccctgca gcgagggcga
     4621 agatgatgtt tccggtcatg tgcgcgatat cgaagggaat tgctcttacc tcgagtgcaa
     4681 ggtaccggtc cagggagatt tcccccccga atgcgaccat cgtctcaaga ttcatccagg
     4741 cgccgaaaac gaccccggcc agcccgcaga aggcggccag ggtccagcga ttgacccttc
     4801 cccgtgccag atgaaagagg acaccgccag ccacaccgca catgccccag gcaaccatct
     4861 gccagggcgt ccagactccc tggccgagcc agaagtttga gaccagcccg cccagggcac
     4921 cgaccgcgaa ccccggggcc ggcccgagca cgaagccggc gatgatcacg atgtcggtgg
     4981 tcgcgaccac gttcgggatc ggcgcgagca caatccggcc ggccaccgcc agcgcggcca
     5041 gcacggcgac cagggcgacg atctgggacg gcgggcgcga acgctcgtac cagagcatcc
     5101 cggccccgag cgtgaacagc aacaccacca cgctgaccag ctgccagctc atgtttccct
     5161 cttcaggaac tcgatcccct gctcgaccga aaccaggcca ggctggtcga gtacccgggc
     5221 ggcctcggag gcgaagtacc agcctccgct gagcacggtc ggcatttcgg cgtcggccac
     5281 cacctcgccg tcaccgagca ggacgacccg atcggcgaat gaaaccgcga attcgacgtc
     5341 atgggtcgag atcacgaccg cggcgccctg accggacagg cgtccggcaa gttcggtcaa
     5401 gtgtccc
//
LOCUS       DAOQKN010000169         5400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_8105_length_5400_cov_5.295978, whole genome shotgun sequence.
ACCESSION   DAOQKN010000169 DAOQKN010000000
VERSION     DAOQKN010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5400
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8105_length_5400_cov_5.295978"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..346)
                     /gene="gatC"
                     /locus_tag="PLJ64_10215"
     CDS             complement(56..346)
                     /gene="gatC"
                     /locus_tag="PLJ64_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HNC93899.1"
                     /translation="MIEREQVLHVARLSRLRLGDEEIDTLAGELSSILDHVDKLAEVD
                     IEGVEPTSHVVPLENVLRPDEPRPSLDRELALSQAPDPHDGAFRVPSPQAEA"
     gene            complement(393..743)
                     /locus_tag="PLJ64_10220"
     CDS             complement(393..743)
                     /locus_tag="PLJ64_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93900.1"
                     /translation="MKLSRVHPFEWLVGLIGLILVAGIVSTVSSPGTLHVVVLLVAAA
                     GAMLPVTVSMSARTNVPIIFETMLWIVSILVSLILLFKAIFPSDVVFESGWLTLAGML
                     AMSFALWRSVAREQ"
     gene            complement(740..1591)
                     /locus_tag="PLJ64_10225"
     CDS             complement(740..1591)
                     /locus_tag="PLJ64_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HNC93901.1"
                     /translation="MSAKIIDGKAVSAEVRERVAAEVSEFIEGGGHQPVLATVLVGDD
                     PASHVYVANKHKACEAAGIGSVHHELPAETSQADLVTLVEALGDDDAIDGILVQLPPP
                     EHIDPDAVIAAINPAKDVDGLTATNAGLLAGGRPGLVPCTPAGVIELLDHAGTELEGA
                     EAVIVGRSNLVGRPLISLLLGRNATVTAAHSRTRDLAAVCSRADVLVAAVGVPELLGA
                     DHVKPGATVIDVGINRTDDGLKGDVDFEAAVEVAGAITPVPGGVGPMTIAMLMANTLA
                     AARARAI"
     gene            complement(1619..3952)
                     /locus_tag="PLJ64_10230"
     CDS             complement(1619..3952)
                     /locus_tag="PLJ64_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNC93902.1"
                     /translation="MEAASNYESGTPDSPGPDEAGVGASAHLFADGLNPPQREAVEYG
                     DGPLLVIAGAGSGKTRVLTHRIAHLLAARRARPGEILAITFTNRAATEMRERVEQLIG
                     ARARAMWVTTFHSACGRMLRGDAEKLGYARAFTIYDEADSIRMIKRVMEELDVDPKRF
                     PARAIKNAISGAKNELIDPEEYKRRGGSYFEDTAAGVYALYEQRMVESNAMDFDDLLV
                     RMVNVLELFPEVRERWRRAFRYVLVDEYQDTNHAQYRLLQLLCGEHRNLTVVGDEDQS
                     IYGFRHADIRNILDFGEDFPDAKVVKLEQNYRSTQTILSAANAVVENNRDRRPKKLWT
                     DLGQGDEIEVVRLSDEHEEARWVAGEIERLVEDEGISPLDVAVFYRMNAMSRVLEDTL
                     VRFGTAYRVIGGTRFYDRAEIKDAIAYLQYLANPRDSVAFTRIVNSPKRGIGNTTQGR
                     LLAFANTTGDDIWDVASQVESVPGMGTAAIRAVSEFQRTMRGLRDDAEGRPLADLLER
                     VLDETGYFEALESERTVEAEGRVENLRELIGVAGEFDTNRALEGESEVEPLDEFLQQI
                     SLYSEQDKLTEREELLTLMTLHNAKGLEYDTVFIIGCEDGIFPHQRALDEGEEEEERR
                     LCYVGITRARKRLYLTSARTRRIFGGRSEATMPSRFLDELPAKLVHQQGSAVGQGGFS
                     TGYSGSGYGGSGRARSYQAPGRPRPDGRSGGPAATRIEPGTAVHFEVGDDVTHGTFGD
                     GVVIGAEPGGVIIVRFANDGSERKLMAEYAPIRKKSN"
     gene            complement(4036..4773)
                     /gene="cysE"
                     /locus_tag="PLJ64_10235"
     CDS             complement(4036..4773)
                     /gene="cysE"
                     /locus_tag="PLJ64_10235"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HNC93903.1"
                     /translation="MADDVITRTFGGVRSHIAAARGRDPATANVSSLEILLTWPGVQA
                     LLAYRLANRLRRSGIPLLPYAVSYLSRMVTGIEIHPDAKIGPGLFIDHGSGVVIGETA
                     EIGENVTLYQGVTLGGTGFQAGKRHPTIEDNVTIGSGAKLLGPIKVGHGAKIGANSVV
                     VEDVPPRSTVVGNPGHPVRIEGRKVTDGPDADWIHLPDPLQDAVKSLSARIKDLERRV
                     AELSGEPKPDASEPEDEPKKGRSSAGG"
     gene            complement(4804..>5400)
                     /locus_tag="PLJ64_10240"
     CDS             complement(4804..>5400)
                     /locus_tag="PLJ64_10240"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HNC93904.1"
                     /translation="YGAEVQETSSMGGMDEAVTLAERIASQRDGYMPLQFSNPANPEA
                     HFRSTGPEIWTDTGGEVDAFVCGVGTGGTITGVARYLKDQGSDARIYAVEPASSPVLS
                     GGRLGPHRIQGIGAGFVPEVLDMDLVDEVIPVSDDDALRTAKQLASTEGILGGISAGA
                     NVHAALELAARPELAGGRIVTVICDSGDRYMSLPFFGT"
BASE COUNT          855 a         1826 c         1766 g          953 t
ORIGIN      
        1 atcatcgcgg ccgtttcgcc gccgctggca gccagaagtt caatatcgga cacggctatg
       61 cctccgcctg cggcgacggc actcggaagg caccgtcgtg ggggtcggga gcctgcgaaa
      121 gggcgagttc ccggtcaaga ctggggcgcg gttcatccgg ccgcagcacg ttctccagcg
      181 gcaccacatg cgaggttggt tcgaccccct cgatgtccac ctccgccagt ttgtcgacgt
      241 ggtcgagaat cgaggaaagc tcaccggcga gggtgtcgat ctcttcgtcg ccaagtcgca
      301 gccgggagag ccgggcgaca tgcagaacct gttcgcgctc aatcacgatg gtgagtctaa
      361 gggcggcgaa gcgagtacgt cagcccgccg ggctactgct cccgggcgac gctgcgccac
      421 agtgcgaagc tcatggcgag catccccgcc agggtcagcc agccggactc gaagacaacg
      481 tccgacggga atatggcctt gaacaggaga atcaagctga ccagaatgga gacgatccag
      541 agcatcgtct cgaagatgat cggcacgttc gtccgcgcac tcatcgacac cgtcaccggc
      601 aacatggccc ctgccgcggc gaccagaagg accaccacgt gcagagtgcc gggcgaggag
      661 acagtcgaga caattccggc caccaggatc aggccgatca atccgacgag ccactcgaac
      721 ggatggacgc gggagagctt caaatggccc ttgcccttgc ggcggcgagc gtgtttgcca
      781 tcagcatcgc gatcgtcatc ggcccgaccc cgcccggcac cggggtgatc gcgccggcca
      841 cctcgaccgc ggcctcgaag tcgacatcac ccttgaggcc gtcgtcggtg cggttgatgc
      901 cgacgtcgat cacggtcgca ccgggtttga cgtggtcggc gcccaaaagt tcgggtacgc
      961 cgaccgcagc gaccagcacg tcggcccggg agcagacggc ggcgaggtca cgggtccggg
     1021 agtgagcggc ggtgacggtt gcgttgcggc ccagcagcag gctgatcagc ggcctgccga
     1081 ccaggttcga acggccgacg atcaccgcct cggccccttc gagttcggtt ccggcgtggt
     1141 cgagcagctc gatcaccccg gccggtgtgc agggcaccag accgggccgg ccgccggcca
     1201 gcagtccggc gttggtcgcg gtcaggccgt cgacatcctt ggccgggttg atcgcggcga
     1261 tcaccgcgtc gggatcgatg tgttccgggg gcggaagctg gacgagaatt ccgtcgatcg
     1321 cgtcgtcatc gccgagcgcc tcgaccagcg tgaccaggtc ggcctggctg gtttcggcag
     1381 gcagttcgtg gtgaaccgac ccgatcccgg ctgcttcgca tgccttgtgc ttgttcgcga
     1441 catagacatg ggacgcgggg tcgtccccga ccagcaccgt ggccagcacc ggctgatggc
     1501 cgccgccttc gatgaactcg ctcacttcgg ccgcaaccct ctcgcgaacc tcggctgaaa
     1561 cagctttgcc gtcaatgatc tttgcgctca accgcctgac cttccgtccg cttgcttttc
     1621 agttcgactt cttccggatc ggcgcgtact cggccatcag cttccgctcg ctgccgtcgt
     1681 tcgcgaaccg gacgatgatc actccgccgg gttcggcgcc gatcacgacc ccgtcaccga
     1741 aggtgccgtg ggtcacatca tcgccaacct cgaaatgaac cgccgtgccg ggctcgatcc
     1801 tggtcgccgc cgggccgcca gaccttccgt cgggccgcgg gcgtccgggg gcctggtagc
     1861 tgcgggcccg gccgctgcca ccgtagccgc tacccgaata gccggtcgag aagccgccct
     1921 gcccgaccgc cgaaccctgc tggtgaacca gcttcgccgg cagttcatcg agaaaacggg
     1981 atggcatggt cgcctcggag cggcccccga agatccgcct ggtccgggcg ctcgtcaggt
     2041 aaagccgttt cctggcccgg gtgatgccga cgtaacagag ccgccgttcc tcttcctctt
     2101 caccttcgtc gagggcccgc tggtggggga agatcccgtc ttcgcagccg atgatgaaga
     2161 ccgtgtcgta ctcgaggccc ttggcgttgt gaagggtcat cagggtaagc agctcttccc
     2221 gttcggtcag cttgtcctgc tccgagtaga gcgagatctg ctggaggaac tcgtccagcg
     2281 gttcgacctc gctttcaccc tcgagcgcgc ggttcgtgtc gaactcgccg gccacgccta
     2341 tcagttcgcg gaggttctcc accctgcctt cggcttccac ggtccgttcc gactcgagcg
     2401 cctcgaaata cccggtctcg tccaggaccc gctccagcag gtcggccagc ggccggccct
     2461 cggcgtcatc tctcaggccc cgcatggttc gctgaaactc ggacaccgcc cggattgcgg
     2521 cggtccccat gccgggaaca ctctcgacct ggctggccac gtcccagatg tcgtcaccgg
     2581 tcgtattggc gaaggcgagc aaccggccct gggtggtgtt gccgatgccg cgcttggggg
     2641 aattgacgat ccgcgtgaag gccaccgagt cgcgcgggtt tgcgaggtac tggagatagg
     2701 cgatcgcgtc cttgatctcg gcccggtcat agaagcgggt gccgccgatc acccggtagg
     2761 cggtgccgaa ccggaccagc gtgtcttcga gaacccggct catcgcgttc atccggtaga
     2821 agaccgccac gtcgagcggc gagatgccct cgtcctcgac cagccgttcg atctcgcccg
     2881 ccacccagcg ggcttcctcg tgctcgtctg agagccgcac gacctcgatt tcgtcgccct
     2941 ggccgaggtc ggtccagagc ttcttcgggc gccggtcgcg gttgttctcg accaccgcgt
     3001 tggcggcgga aaggatggtc tgggtcgaac ggtaattctg ttcgagcttg accaccttcg
     3061 cgtccggaaa gtcctcaccg aagtcgagga tgttgcggat gtcggcgtgg cggaagccgt
     3121 agatcgactg gtcctcgtcg ccgaccacgg tcaggttccg gtgctcgccg cagagcagct
     3181 gaagcagcct gtactgggcg tggttggtgt cctggtattc gtcgaccagc acgtagcgga
     3241 aggcccgccg ccaacgctcc ctcacctcgg gaaacagttc cagcacgttg accatgcgga
     3301 ccaggaggtc atcgaagtcc atcgcgttgg actcgaccat gcgctgctcg tagagcgcgt
     3361 acactcccgc cgccgtgtcc tcgaagtagg aaccgccccg ccgcttgtac tcctcggggt
     3421 cgatcagctc gttcttggcc ccggagatcg cgttcttgat cgcccgggcg gggaagcgct
     3481 tcgggtcgac atcgagctct tccatgaccc gtttgatcat gcggatcgaa tcggcctcgt
     3541 cgtagatcgt gaaggccctt gcgtatccga gcttctcggc gtcaccgcgc agcatccggc
     3601 cgcaggccga gtggaaagtg gtgacccaca tggcgcgggc ccgggcaccg atcagctgct
     3661 cgactcgttc ccgcatctcg gtcgccgccc ggttggtgaa ggtgatcgcg aggatctccc
     3721 cgggccgggc ccggcgggcc gccagcaggt gcgcgatccg gtgggtcagc acgcgggtct
     3781 tgccggaacc ggccccggcg atcaccagca gggggccgtc gccgtattcg actgcttccc
     3841 gctggggcgg gttcaacccg tcggcaaaca gatgtgccga cgcgccgacc ccggcctcgt
     3901 ccggaccggg cgagtcgggg gtaccgctct cgtagttcga tgccgcttcc acccgtcgag
     3961 gatatctacc tctccggcgg ggccggactc ccgggttgac cggttatgcg gcgctcccgg
     4021 ccccccgagg ggaactcatc cgccggccga gctccggccc tttttcggct cgtcctccgg
     4081 ttcgcttgca tccggtttcg gctcgccgga aagctcggcg acccgccttt cgaggtcctt
     4141 gatccgggct gaaagcgact tgaccgcatc ctgcagcggg tccgggaggt gaatccagtc
     4201 ggcgtccggc ccgtcggtga ccttgcggcc ttcgatccgt accggatggc ccgggttgcc
     4261 gaccacggtc gagcgcggag ggacgtcctc gaccaccacc gagttggccc cgattttggc
     4321 cccgtgacct accttgatcg gaccgagcag cttcgctccg gatccgatcg tcacgttgtc
     4381 ctcgatggtc gggtgccgtt ttccggcctg gaagccggtc ccgcccagcg tcacgccctg
     4441 gtagagcgtc acgttctccc cgatctccgc ggtttccccg atcaccacac cggagccgtg
     4501 gtcgatgaag aggcccggcc cgatcttcgc gtcaggatgg atttcgatcc cggtgaccat
     4561 tcggctcaga tacgagaccg catagggcaa aagcgggata cctgatcgtc tcagccggtt
     4621 tgccagccgg tacgcgagca gcgcctgaac acccggccag gtgaggagga tctcgaggct
     4681 ggagacattg gcagtcgccg gatcacggcc gcgggcggcg gcgatgtggg agcgcacgcc
     4741 cccgaaggtc cgggtgatca cgtcatcagc catccggaaa gcctagagcc tcggctttcc
     4801 ggctcaggtt ccgaagaagg gcagtgacat gtaccggtcg ccgctgtcac agatcacggt
     4861 gacgatgcga ccgccggcca gttccggcct tgcggccagc tcgagggcgg cgtgaacgtt
     4921 ggcgccggcc gaaataccgc cgaggatgcc ctccgtcgag gcgagttgct tggcagtgcg
     4981 aagcgcgtcg tcgtcgctga ccggaatcac ttcgtcgacc aggtccatgt cgagaacctc
     5041 cggcacgaat ccggccccga tcccctggat ccggtggggg ccaagtcggc cgccggaaag
     5101 caccggagac gaggcgggct cgaccgcgta gatccgggcg tcggaaccct ggtccttcag
     5161 gtaacgggcc acgccggtga tcgtgccgcc ggttcccacg ccgcagacga aggcatcgac
     5221 ctcgccaccg gtgtcggtcc agatctcggg accggtcgac cggaagtgag cctcgggatt
     5281 ggcggggttc gagaactgaa gcggcatgta gccatcgcgc tgggaggcga tcctctcggc
     5341 cagggtcacc gcttcatcca tgccgcccat cgacgaggtt tcctgaacct cggcgccgta
//
LOCUS       DAOQKN010000170         5322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_8289_length_5322_cov_6.329599, whole genome shotgun sequence.
ACCESSION   DAOQKN010000170 DAOQKN010000000
VERSION     DAOQKN010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5322
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8289_length_5322_cov_6.329599"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2498)
                     /locus_tag="PLJ64_10245"
     CDS             complement(<1..2498)
                     /locus_tag="PLJ64_10245"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HNC93905.1"
                     /translation="MPLLEPDVMKISPEASEPALDRAPDWVAGGTPEPLRSRLTALLG
                     EEKLLGRATDLIRYASDASPYRMVPKAVVMAHSAEDVAKVLEFGRREGIPVTFRSGGT
                     SLNGQSQTEGILVDVRRHFTGVEVIDDGRQVRVGPGTVLGHANKVLAPYGRKLGPDPA
                     STDIATVGGVVNNNSGGMRCGVTYDSYQTVTGMTFVLPSGTMINTEDENAEADFAAKE
                     PELAEGLMKIREEILADQVLVDRIRHKFEIKNTTGYRLCAFLDGETPVEIFRRLIIGS
                     EGTLAFVASVTFDTVELPVLTTTAWLHFPDIQTAVDPVPELVAAGATAVELMVAPALI
                     TAGWNMPGAPEYFRELDPESAALLVEFGGPDEASLDAQEARALEIVDAEKLILPASFG
                     RDEESIEINWKVREGLHGLIGKMRLPGTALIIEDVCVPPARIAEAAVELRGMLAEHGF
                     LAGVAGHASAGNLHFMLTPDFSKPEDTERYETFMNALVDLIIDKYDGALKAEHGTGIN
                     MAPYVIREWGETAVDFMWRAKQLADPENALNPGSILTRDRQAHLQNLKTTPPVEEIAN
                     ACVECGFCEPVCPSRHLTTTPRQRIVLRREMVRQGEDTPVRRALLQEFEYEAIETCAA
                     DGSCLLACPVGIDTGKFIKGFRAEQHSPAAEKMALRAARRWDRIEKLARTGLKLGGAV
                     SSVASDGAMRGLTRAARKAMSADLVPEWGEDMPPAAQPKLPQTTSHGAAAVYMPACIN
                     RMFGRSKDDPGKSLPEAMVEVSARAGKPVWIPPDIAGTCCSVPWSSKGYRSAHEFKAN
                     QTVERIWRWTGGGTLPLVIDASSCGHGLIDFGEGV"
     gene            complement(2523..4118)
                     /gene="mdlC"
                     /locus_tag="PLJ64_10250"
     CDS             complement(2523..4118)
                     /gene="mdlC"
                     /locus_tag="PLJ64_10250"
                     /EC_number="4.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF005485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoylformate decarboxylase"
                     /protein_id="HNC93906.1"
                     /translation="MPTVREATFDLFRKLGMTTIFGNPGSTELPMLADYPDDFEYVLG
                     LQEAAVVGMADGHAQVTGKPTLVNLHTAPGLGNAVGALFNAQANHSPVVVTVGQQVRA
                     QVTMQANLTNRDAIRVGDPFVKYSYEPARAEDVPLAIAHAIHLASLPPMGPVVVSIPM
                     DDWDVEMNAAEAQHVIDRAVVGRSGADPTVVADLAARLKQAENPVFVAGPDIDAAGGW
                     DEAIALAEGQNLPVWASPATGGGRLGFPESHPHWRGVLPPGIAAIAEVLKEHDLILVV
                     GSSVFPYYPYMPGDFLGENSKLVQVTSDPDEAARAPMGEAIVADVKLTLAALNGELDG
                     TERPAIEPWPGPTRMEETDPLNPSVVMNTLKDVMPEDAVIVLEAPTSTMALRNQLRIS
                     KPNSYFFGAGGGLGFGLSSAVGIGYAEDRPVICVLGEGSVQYAVTAFWTAAAYDIPVT
                     FLILRNEEYGILKWFAEVEQVSDAPGLDLPALETAKIAEGYGIESTTAKSAEEVAAAL
                     KEAIADSRPRLVEVPVEPGMSLF"
     gene            4202..4714
                     /locus_tag="PLJ64_10255"
     CDS             4202..4714
                     /locus_tag="PLJ64_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93907.1"
                     /translation="MRRTLVISLILPALALPFAGCSDGRKVATPPACLSASATWLTAL
                     SSAPDQVLLEGVTPLSDCLTKGQSAAQHEETGKTAVETATQLAAFYKAGEKAGGNGTG
                     PGGDTSEQAAVMAGYLVGAIEKGAEDTEGIHAALVDRVEAAATNGLEGASQQIQGAYQ
                     KGYAAGQAKG"
     gene            4784..>5322
                     /locus_tag="PLJ64_10260"
     CDS             4784..>5322
                     /locus_tag="PLJ64_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HNC93908.1"
                     /translation="MSEQLWGGETTKAIENFPVSGEKIPPSLVKWLGLIKANAARANA
                     ELGLLDTDIADRIAKAGDEVGAGKHDDQFPIDIFQTGSGTSSNMNANEVIANLAGEGV
                     HQNDHVNMGQSSNDVFPSAVHLAALDQVTHDLLPAMEQLAVALEAKAEEFRDVVKAGR
                     THLMDAVPVTLGQEFGGYAS"
BASE COUNT          851 a         1768 c         1826 g          877 t
ORIGIN      
        1 accccctcgc cgaaatcgat caggccgtgg ccgcaggaag aggcgtcgat caccagcggc
       61 agggttcccc cgccggtcca gcgccagatc cgctcgaccg tctggttcgc cttgaactcg
      121 tgggcgctcc tgtagccctt cgagctccac ggaaccgaac agcaggtgcc ggcgatgtcc
      181 ggcgggatcc agaccggctt gccggcccgg gccgagacct cgaccattgc ctcgggcagg
      241 ctcttccccg ggtcatcctt ggagcggccg aacatgcggt tgatgcaggc cggcatgtac
      301 acggcggcgg cgccatggga ggtcgtctgt ggcagcttcg gctgggcggc cggcggcatg
      361 tcctcgcccc attcgggaac caggtccgcg ctcatcgcct tgcgtgcggc gcgggtcagg
      421 ccccgcatcg ccccgtccga agcgacgctg gaaacggcac ccccgagctt gaggccggta
      481 cgggccagct tctcgatccg gtcccagcga cgggcggccc gcagggccat cttctcggcc
      541 gcgggactgt gctgctcggc ccggaaaccc ttgatgaact tgccggtgtc gatcccgacc
      601 gggcaggcca gcaggcagga accgtccgcg gcgcaggtct cgatcgcctc gtactcgaac
      661 tcctggagca gggcccggcg gaccggggtg tcctcgccct ggcggaccat ctcccggcgc
      721 agaacgatgc gttgccgcgg ggtggtggtc aggtggcggc tggggcagac cggctcgcag
      781 aaaccacatt cgacgcaggc gttggcaatc tcctcgaccg gcggggtcgt cttcaggttc
      841 tgcaggtggg cctgcctgtc ccgggtcagg atcgagccgg gattcagcgc gttctccggg
      901 tcggccagct gcttggcccg ccacatgaag tcgaccgccg tttcgcccca ctccctgatc
      961 acgtacggag ccatgttgat cccggtgccg tgctcggcct tgagcgctcc gtcgtacttg
     1021 tcgatgatca gatcgaccag ggcgttcatg aaggtctcgt agcgctccgt gtcttccggc
     1081 ttcgagaagt ccggggtgag catgaagtgc aggttgccgg ccgaggcgtg gccggcgacg
     1141 ccggcgagga agccgtgttc ggccagcatg ccgcgcagct ccactgccgc ctcggcgatc
     1201 cgggccggcg gcacgcagac atcctcgatg atcagcgcgg tgccgggcag gcgcatcttg
     1261 ccgatcaggc cgtggaggcc ctcgcggacc ttccagttga tttcgatcga ttcctcgtcc
     1321 cggccgaagc tggccggcag gatcagcttc tccgcatcga cgatctccag cgccctggct
     1381 tcctgggcat cgaggctggc ctcgtcgggc ccgccgaatt cgaccagcag tgcggccgat
     1441 tccggatcga gctcgcggaa gtactcgggg gcgccgggca tgttccagcc ggccgtgatc
     1501 agggccgggg cgaccatcag ctcgaccgcg gtggcccccg ccgcgaccag ctcgggaacc
     1561 gggtcgaccg cggtctggat gtcggggaag tggagccagg cggtggtggt gagcaccggc
     1621 aactcgaccg tgtcgaaggt gacgctggcg acgaaggcga gggtgccttc cgaaccgatg
     1681 atcaggcggc ggaagatctc gaccggggtc tcgccatcga ggaaggcaca gagccggtac
     1741 ccggtggtgt tcttgatttc gaacttgtga cggatccggt caaccagcac ctggtcggcg
     1801 aggatctcct cgcggatctt catcaggcct tcggccagtt ccggttcctt cgcggcgaaa
     1861 tccgcttccg cgttttcgtc ttcggtgttg atcatcgtgc ccgagggcag gacgaaggtc
     1921 atcccggtga ccgtctggta cgagtcgtag gtcaccccgc agcgcatgcc gccggagttg
     1981 ttgttgacca cgccgccgac cgtggcgatg tccgtcgaag ccggatccgg gccgagcttg
     2041 cgaccgtagg gggcgagcac cttgttggcg tggccgagca cggttcccgg tccgacccgg
     2101 acctgccggc cgtcgtcgat cacctcgacc ccggtgaagt gcctgcgcac gtcaaccagg
     2161 atgccctcgg tctggctctg gccgttgagg ctggtgccgc cggagcggaa ggtgaccggg
     2221 atcccttcgc gccggccgaa ctcgagcact ttggccacgt cctcggcact gtgggccatc
     2281 accaccgcct tcggcaccat ccggtagggg ctggcgtccg aggcgtagcg gatcaggtcg
     2341 gtcgcccggc cgagcagctt ctcctcgccg agcagcgcgg tgagccggga gcggagcggt
     2401 tcgggcgtgc cgcccgcaac ccagtcggga gcccggtcga gtgccggctc gctcgcctcc
     2461 gggctgatct tcatgacatc cggttcgagc agtggcatct cagactccct gacagttggt
     2521 ttctagaaca gcgacattcc cggctcgacc ggcacttcga ccagccgcgg cctggaatcc
     2581 gcgatcgctt ccttcagcgc cgcggctact tcctcggcac tcttcgccgt cgtgctctcg
     2641 attccgtacc cctcggcgat cttcgccgtc tccagggccg gcaggtcgag cccgggggca
     2701 tcggaaacct gctcgacctc ggcgaaccac ttgaggatcc cgtactcctc gttgcgaagg
     2761 atcaggaagg tgaccgggat gtcataggcg gcggcggtcc agaaggcagt caccgcgtac
     2821 tgcacagaac cttcgccgag cacgcagatc accgggcgat cctcggcgta accgatgccg
     2881 accgcggagg aaaggccgaa gccgagtccg ccgccggccc cgaagaagta gctgttcggc
     2941 ttcgagatcc ggagctggtt gcggagggcc atggtcgagg tcggagcttc gagcacgatc
     3001 accgcgtctt ccggcattac gtccttcagc gtgttcatca cgaccgacgg gttgagcgga
     3061 tcggtctctt ccatccgggt cggccccggc cacggttcga ttgccggccg ctcggtgccg
     3121 tcgagctcgc cgttcagggc ggccagggtc agtttcacgt cggcaacgat cgcctcgccc
     3181 atcggggcgc gggccgcctc gtccgggtcg gaggtgacct ggaccagctt cgagttctcg
     3241 ccgaggaagt cgccgggcat gtacgggtag tagggaaaga cggacgagcc gaccaccagg
     3301 atcaggtcat gttccttcag cacctcggcg atcgcggcaa tgccgggcgg cagcaccccg
     3361 cgccaatgcg ggtgactttc cgggaagcca agccggccgc caccggttgc gggggaggcc
     3421 cagaccggca ggttctggcc ttcggccagg gcgatcgctt cgtcccagcc gccggccgcg
     3481 tcaatgtcgg gtccggccac gaagaccggg ttctctgcct gcttgaggcg ggcggcgagg
     3541 tcggcgacca cggtcgggtc ggcgcccgat cgtccgacca ctgcccggtc gatcacatgc
     3601 tgtgcctcgg ctgcgttcat ctcgacatcc cagtcgtcca tcggaatcga aacgacgacc
     3661 ggccccatcg gcggaagcga cgcgaggtga atcgcgtggg cgatcgccag cggcacgtct
     3721 tcggcgcggg cgggttcgta ggagtacttg acgaaggggt ccccgacgcg gatcgcgtcg
     3781 cggttggtca ggttcgcctg catcgtgacc tgggcgcgaa cctgctggcc gaccgtgacc
     3841 accaccggcg agtggttggc ctgcgcgttg aagagcgcgc cgaccgcgtt gccgaggccc
     3901 ggagcggtgt ggaggttgac cagggtcggc tttccggtga cctgggcgtg accgtcggcc
     3961 atgcccacca cggccgcctc ctgcaggccc agcacgtact cgaagtcatc ggggtaatcg
     4021 gccagcatcg gcagctcggt cgagcccggg tttccgaaga tcgtggtcat ccccagcttg
     4081 cggaaaaggt cgaaggtcgc ttcgcgaacg gtaggcatgg gcgcagccta tatgtgaacg
     4141 ggtgagtacg ggaccggcag gcaagggtgg ggcgaaggtc ctggcctgtc aggattgtgc
     4201 cgtgcgcaga actctcgtga tcagtctgat ccttcccgcc ctcgccctgc ccttcgccgg
     4261 ctgcagcgac ggtcgcaagg tcgcgacgcc gcccgcctgt ctgagcgcct ccgcgacctg
     4321 gttgacggca ctttcttcgg caccggatca ggtcctgctc gaaggggtca cgccgctcag
     4381 cgactgcctg accaaggggc aatccgcggc ccagcatgaa gaaaccggca agaccgcggt
     4441 agagacggcc acccagctgg ccgccttcta caaggccggc gaaaaggcgg gcggcaacgg
     4501 gaccgggccc ggtggcgaca cctccgaaca ggcggcggtg atggccggtt acctggtcgg
     4561 ggcgatcgag aagggggccg aagacaccga aggtattcac gccgccctgg tcgaccgggt
     4621 cgaagccgcc gccaccaacg gactcgaagg ggccagccag cagattcagg gcgcctacca
     4681 gaagggttac gcggccggtc aggcgaaggg ctaggcgaac gccgttccgg tgccgggctg
     4741 aaagactgtc cgcagcaagt cagtcaaagc cggaaggaca gaagtgagcg agcaactctg
     4801 gggtggcgaa accaccaagg cgatcgaaaa tttccccgtg tccggcgaga agattccgcc
     4861 ctcgctggtc aagtggctgg ggctgatcaa ggccaacgca gcccgtgcga acgcggaact
     4921 gggactgctc gacaccgaca tcgccgaccg gatcgcgaag gccggggacg aggtcggagc
     4981 gggcaagcat gacgaccagt tcccgatcga catcttccag accggttccg gcacctcctc
     5041 gaacatgaat gcgaacgagg tgatcgccaa cctggccggc gaaggagtcc accagaacga
     5101 ccacgtgaac atgggccagt cgtcaaatga cgtcttcccc tcggccgttc acctcgctgc
     5161 cctcgatcag gtcacccacg acctgctgcc ggcgatggaa cagctggcgg ttgcgcttga
     5221 agccaaagcc gaggagttca gggacgtggt caaggccggg cgcacccacc tcatggacgc
     5281 ggttccggtc accctcggcc aggagtttgg cggctacgcc tc
//
LOCUS       DAOQKN010000171         5315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_8305_length_5315_cov_4.823194, whole genome shotgun sequence.
ACCESSION   DAOQKN010000171 DAOQKN010000000
VERSION     DAOQKN010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5315
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8305_length_5315_cov_4.823194"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="PLJ64_10265"
     CDS             complement(<1..271)
                     /locus_tag="PLJ64_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93909.1"
                     /translation="MTGALPAFLAGIAAVLAVVAVGEALGARTPADEGQGPGRGDRAG
                     KETGGGGAGLRAWLAATVEPLRRARREGYLPTSDERFRLALLFSFA"
     gene            complement(268..1341)
                     /locus_tag="PLJ64_10270"
     CDS             complement(268..1341)
                     /locus_tag="PLJ64_10270"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HNC93910.1"
                     /translation="MDDRGRLAAEIRAGLIERRRAGAGTDQEGIEQAIRHAVEARAVM
                     LSEAAQEDLVERILREASGLGPLEVLLDDGSVEEVMVNGPDLVYVERGGKIEPAGVRF
                     EDEEQLRNTIERILAPLGRRVDELSPMADARLPDGSRVNVVIPPLAVDGPAVSIRRFP
                     GRRPTVDDLVQWGTLSAGQAERLGREVAARRSILISGGTGSGKTTLLNALSGFIDAAE
                     RIVTIEDAAELSLAQPHVVRLESRPPSIEGRGEVTIRDLLRNALRMRPDRILIGEVRG
                     AEALDLLTALNTGHDGALSTVHASSATDALARIEVLALMADVGLPLNVIGAQLRRGID
                     LVVHISRTAEGGRKVTEIEEVGQ"
     gene            complement(1343..1957)
                     /locus_tag="PLJ64_10275"
     CDS             complement(1343..1957)
                     /locus_tag="PLJ64_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93911.1"
                     /translation="MLLAAAGAGILAVSLVGGYSSSVAESYGELRPVVVLTRGLAPGQ
                     VISPKVAAASFEIRQVPARFVPAEALGDPTRAVGLEPVGPMPAGSYLTGPGLMVPGSD
                     RPKRRPLGHGRHAVEISVAGAGALSGGGKVDVLVTTDNDRGGGRTLVAARRVPLISIG
                     RTGQSEAGPGLTEVTLGLTRGEAIRLVDAQTFARRITVLPPAGP"
     gene            complement(1991..2731)
                     /locus_tag="PLJ64_10280"
     CDS             complement(1991..2731)
                     /locus_tag="PLJ64_10280"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNC93912.1"
                     /translation="MSIGLADLIPLLAALFVLAGREAWLAHRRVLVNRALHEARRPLQ
                     AIALSLPAGPGRMTPVLPVWQAIQALGEVDRQINGGAPDRPRPEPIACRLMTEACVRR
                     WQSRARMAGGGIEMRWTGPEALVTGDPAALSAAIENLIVNAIEHGGPRIVVRALVIGK
                     RLRLEVTDNGKAHRAAGRSGSPAEVIARLRGKDRHGHGLPVVREAAREHAGRFEINLD
                     ESGSTATLTLPCATRAPAPAPGLVGTEA"
     gene            complement(2743..3453)
                     /locus_tag="PLJ64_10285"
     CDS             complement(2743..3453)
                     /locus_tag="PLJ64_10285"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC93913.1"
                     /translation="MLPVREGIARIVVCEDDQPTLELLCEHLTADRFSVLAAPSAADA
                     MRLCLYNDPDLMLLDLGLPDAAGLDLLREIRNPDRTVNRVDPGIGVIIVSGRSTERDR
                     VRGLSEGADDYVQKPFSYGELLARINSVLRRRRRSHSLVQVGEIEINDVEHTVRVGGR
                     YLRLSMKEYALLKILANEPSRVFSKEELLQEVWGYRSSGHTRTLEAHCSRLRKKLDPK
                     HGRYVVNCWGVGYRLVGP"
     gene            complement(3453..3878)
                     /locus_tag="PLJ64_10290"
     CDS             complement(3453..3878)
                     /locus_tag="PLJ64_10290"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HNC93914.1"
                     /translation="MAIGADITRPARRFRRLERRRVHGFEVMVAGDPVSRLLGLALLD
                     RHRAPEGLLIPRCRSVHTYGMRFPLDLLFLGPDCQPLIAVREVGPGHRVRVPEAAAVL
                     ELPSDWDLEKWEGATGAGSGGEPGTAEPDGPGDPGQGRG"
     gene            4058..4525
                     /locus_tag="PLJ64_10295"
     CDS             4058..4525
                     /locus_tag="PLJ64_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93915.1"
                     /translation="MESKKTRRGNYESGTDYVLEYGNLRFSFNERDFSQRVEQAAVRL
                     GFVTPGLTFAELNDLLHLAVSGEIEEPSSELGIHVTHHWEDLTGPGNQSFVHWLRRLV
                     FRGAWLDQRVKEGELDIAFDEGRQTFGYIQPDRGPEMIELAKEPSWRRVAFRR"
     gene            complement(4616..>5315)
                     /locus_tag="PLJ64_10300"
     CDS             complement(4616..>5315)
                     /locus_tag="PLJ64_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93916.1"
                     /translation="FGQRVLVEAQPRSGRTSFLRSLAKAIARGAGDEAEIIVLLVDER
                     PEEVTAWSREVPEVEIAAAPADLGAHEQVRTAERILAKVKRQAEAGNDVVIIVDSLTR
                     LGNAYGEAGAVKPFFGAGRELEEEGAGSVTVIATALWGSPGDQDVMDAVHTTENATIR
                     LDASLASAGVEPSLDVTACAVSGQESVLDQEQVDAVRRLRAELKDLEPVPAANRLAEL
                     IKSSPSNEELLKNL"
BASE COUNT          829 a         1963 c         1715 g          808 t
ORIGIN      
        1 ccgcgaagct gaacagcagg gccagccgga agcgttcgtc ggaagtcggc aggtagccct
       61 cacgccgggc ccgtcgaagc ggttcgaccg tggccgccag ccaggccctg agcccggccc
      121 cgccgccgcc cgtttctttc ccggcccggt cgcctcttcc cggaccctgc ccctcgtcgg
      181 ccggggtccg ggcgcccagt gcctcgccga cggcaaccac ggcaagcacc gcggcaattc
      241 cggccaggaa ggcgggcagg gcgccggtca ctggccgacc tcctcgattt ccgtgacctt
      301 gcgaccgccc tcggcggtgc gggaaatgtg gacgacgagg tcgatcccgc ggcggagctg
      361 ggcgccgatc acgttcagcg gcaatccgac atcggccatc agggcgagga cctcgatccg
      421 ggccagggca tcggtggccg aactggcgtg aacggttgaa agcgctccgt cgtgaccggt
      481 gttcaaggcg gtcagcaggt ccagggcctc ggcgccgcgc acctctccga tcaggatccg
      541 gtcgggtcgc attctcagcg cgtttcggag caggtcgcga atcgtcactt cgccgcggcc
      601 ctcgatgctt ggcggacggg attcgagccg gaccacatgg ggttgcgcga ggctgagctc
      661 ggccgcgtcc tcgatcgtca cgatccgttc ggcggcatcg atgaatcccg acagggcatt
      721 cagcagcgtg gtcttgccgg aaccggtgcc gccgctgatc aggatgctgc gacgcgccgc
      781 cacctcgcga cccagccgct ccgcctgccc ggcgctcagg gttccccact gaaccaggtc
      841 gtcaaccgtg ggccgccgcc cgggaaagcg gcggatcgaa accgccggcc cgtcgaccgc
      901 cagcggcggg atcacgacgt tcacccgtga accgtccggc agccgtgcat cggccatcgg
      961 gctgagctcg tcgacccgcc tgccgagcgg ggcgaggatc cgctcgatcg tgttccgaag
     1021 ctgttcctcg tcttcgaacc tgaccccggc cggttcgatc tttccgcccc gttcaacgta
     1081 gacgaggtcc ggcccgttga ccatgacttc ctcgacgctg ccgtcatcaa ggagaacctc
     1141 caacggcccc aggccggatg cctcacggag gatccgctcg accagatcct cctgcgccgc
     1201 ttcggagagc atcaccgccc gggcttcgac cgcatgccgg atcgcctgct cgattccttc
     1261 ctgatctgtg ccggcaccgg cccggcgccg ctcaatcaga ccggcccgga tctcggccgc
     1321 cagcctcccc cggtcatcca tctcagggcc ccgccggtgg cagcacggta attcgccggg
     1381 cgaaggtctg cgcgtcaacc aggcgaatcg cttcgccccg ggtcagcccc agggtcacct
     1441 cggtcagtcc cgggccagcc tcgctctgac cggtccggcc gatcgagatc aaagggaccc
     1501 gtcgcgccgc gaccagggtc cggcctccac cccggtcgtt atcggtcgtg accagaacgt
     1561 caacctttcc gcccccgctc agggccccgg ctcctgccac cgatatctcg accgcatgcc
     1621 ggccgtgacc gagcggcctg cgtttcggcc ggtcgctgcc cggcaccatc agcccgggcc
     1681 cggtcaggta gctgccggcc ggcatcggcc cgaccggttc caggccaaca gcccgggtcg
     1741 gatcacccag cgcttccgcc ggtacgaaac gtgccggcac ctgccggatc tcgaaactag
     1801 ccgccgccac tttcggtgag atcacctgcc cgggagcgag accgcgggtc aggaccacga
     1861 ccggcctcag ctcgccgtag ctctcggcga ccgaggagga gtacccgccg accaggctca
     1921 ccgcgaggat tccggcaccg gcggcggcca gaagcatcag cgttgcccgc cggcgccggc
     1981 ccatcccggt tcaggcctcg gtcccgacca gccccggtgc cggagccggg gcccgggtcg
     2041 cgcagggcag ggtcagggtc gcggttgaac cggactcatc caggttgatc tcgaagcggc
     2101 cggcatgttc acgggcggcc tcccggacca ccggcaaccc gtggccgtgc cggtccttgc
     2161 cacgcagcct cgcgatcact tcggccgggc tgccggagcg tcccgcggca cggtgggcct
     2221 tgccgttgtc ggtgacctcc agccgcagcc gcttgccgat caccagggca cgcaccacga
     2281 tccgcggccc accgtgctcg atcgcgttga cgatcaggtt ctcgatcgcc gccgagagcg
     2341 cggccggatc cccggtcacc aatgcctccg gtccggtcca gcgcatttcg atcccgcctc
     2401 cggccatccg ggcccgtgac tgccagcgcc gcacacaggc ctcggtcatc aaccggcagg
     2461 cgatcggctc gggcctcggc cggtcgggtg caccgccgtt gatctgccga tcgacctcgc
     2521 caagcgcctg gattgcctgc cagaccggca gcaccggggt catccggccc ggccccgccg
     2581 gcagggagag cgcgatcgcc tggagcggcc gccgggcttc atggagcgcc cgattgacga
     2641 ggacgcggcg atgggccagc caggcttccc gcccggcaag tacaaagagc gcggccagca
     2701 gggggatcag gtcggcaagt ccaatactca cttcagcacc cctcacggac ccacgagccg
     2761 gtagccgact ccccagcagt tgaccacgta acggccgtgc ttcggatcga gcttcttccg
     2821 cagccggctg cagtgcgctt cgagggtccg ggtgtgcccg gaggagcggt atccccagac
     2881 ctcctgcagc agttcctcct tcgagaagac ccggctcggt tcgttggcga ggatcttcag
     2941 cagcgcgtac tccttcatcg aaagtcgcag gtagcggcca cccacccgga ccgtgtgctc
     3001 gacgtcgttg atctcgattt ccccgacctg aacgagggaa tggctgcgcc gccggcgccg
     3061 cagcaccgaa ttgatccggg ccagcagttc gccgtagctg aagggcttct ggacgtaatc
     3121 gtccgcccct tcgctgaggc cgcgcacccg gtcgcgctct gtgctgcggc cgctgacgat
     3181 gatcaccccg attcccggat cgacccggtt cacggtccgg tccggattgc ggatctcgcg
     3241 cagcaggtcg agcccggccg cgtcgggcaa accaaggtcg agcagcatca ggtccgggtc
     3301 gttgtagagg cagagccgca tcgcatcggc ggcgctcggc gcagccagga ccgagaaacg
     3361 gtcggcggtc aggtgttcgc agagcagttc gagggtcggc tggtcgtcct cgcagacgac
     3421 gatccgggcg atcccttccc gcaccggaag catcagcccc gcccctgccc cgggtcgccg
     3481 ggcccgtccg gctcggcggt acccggctct ccgccagatc ccgcgccggt tgccccctcc
     3541 cacttctcca ggtcccagtc cgagggaagt tccagcaccg ccgcggcttc cggcacccgc
     3601 acccggtggc ccggcccgac ctcccgcacc gcgatcagcg gctggcagtc cggcccgaga
     3661 aagagcaggt ccagcggaaa ccgcatgccg taggtgtgga ccgaacggca ccggggaatc
     3721 agcagacctt ccggcgcccg gtgccggtcc agcagcgcca ggcccagaag ccgcgacacc
     3781 gggtcaccgg ccaccatcac ctcgaaaccg tgaaccctcc gccgctcaag tcgccggaaa
     3841 cgcctcgccg gccgcgtgat atccgcacca atcgccatgc ccctctaagg cacgggcaca
     3901 ggaaactctc ccccgcagaa ccggatcggc tctgcaacga gtctttcggg gtgcaaccgc
     3961 ccgttcaggt ccggcggtgc caagtcccta aaggcagcct caaggcatga tgcccgggca
     4021 cccaaggacc atgccgcggg tgctcaaggc ttcaaccgtg gaaagtaaaa agacccgcag
     4081 aggaaactac gagtcgggca ctgactatgt gctcgagtac ggcaatctca ggttctcgtt
     4141 caacgaacgg gacttcagcc agagagtcga acaggccgca gtgcggctcg gtttcgtgac
     4201 tcccggcctg accttcgccg agctcaatga cctgctccat ctggccgttt ccggcgagat
     4261 cgaggagccg agctcggagt tgggcatcca cgtcacccat cactgggagg acctgaccgg
     4321 acccggcaac cagagtttcg tccactggct gcgccggctc gtcttccgcg gcgcctggct
     4381 cgaccagcgg gtcaaggaag gcgaactgga catcgccttc gacgagggcc gccagacctt
     4441 cggctacatt cagcccgacc gcggccccga gatgatcgag ctggccaaag agccgagctg
     4501 gcgccgcgtc gccttccgcc gatagagaac ttcaagtcgg gcggggtccg acccgccctc
     4561 cattctggac ccgcccctcc cattcgcaca aaacgatggt tgggaggggc tctagctaca
     4621 ggttcttcag cagttcttcg ttgcttgggg agctcttgat cagctcggcc aggcgattgg
     4681 cggccgggac cggttccagg tccttcagtt cagcccggag gcgtcgcacc gcgtccacct
     4741 gttcctggtc cagtaccgac tcctgaccgg aaacggcgca ggcggtgacg tccagtgacg
     4801 gctccacccc ggccgaagcc agcgaggcat cgagacggat ggttgcgttc tcggtcgtgt
     4861 ggaccgcgtc catcacgtcc tggtcgccgg gcgagcccca gagggcggtg gcgatcacgg
     4921 tgaccgagcc ggcaccttcc tcttccagtt cgcgaccggc cccgaagaag ggcttgaccg
     4981 ctccggcctc gccgtaggcg ttgccgagcc gggtcagcga gtcgacgatg atcacgacgt
     5041 cgttgccggc ctcggcctgg cgcttgacct tggccagaat ccgctcggcg gtccgaacct
     5101 gctcgtgggc accgaggtcg gcgggcgcgg cggcgatctc gacctccggc acctcgcggg
     5161 accaggcggt cacctcttcc ggccgctcgt cgaccaggag cacgatgatc tcggcttcgt
     5221 caccggcacc gcgggcgatc gccttggcca gcgaccgcag gaagctggtc cggccggaac
     5281 gcggctgggc ctcgaccagc acccgctggc cgaag
//
LOCUS       DAOQKN010000172         5220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_8536_length_5220_cov_3.248403, whole genome shotgun sequence.
ACCESSION   DAOQKN010000172 DAOQKN010000000
VERSION     DAOQKN010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5220
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8536_length_5220_cov_3.248403"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="PLJ64_10305"
     CDS             complement(<1..548)
                     /locus_tag="PLJ64_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNC93917.1"
                     /translation="MPLVTTERDGPMAVITLNSPPLNLFDRAMTEELMAAFEAIQDDP
                     PRGLLIRAEGDVVSGGVNVNEFQGLTAAEGTRLWEQLLGLIDRVEGFPFPVVFAAHGL
                     TLTAAFELSLACDLIIASESAKFGLVEIVVGLTPSMGGPQRLAERAGSGRARQLVMTG
                     GLFDAETLERWNVVNEVKPDEKL"
     gene            complement(563..1261)
                     /locus_tag="PLJ64_10310"
     CDS             complement(563..1261)
                     /locus_tag="PLJ64_10310"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC93918.1"
                     /translation="MTRLAAGRSTHLEPEEIAAETLRLFDQGGDPSIRQLATALNVTP
                     SAIYHHFDSRSDIVQAAVELVWTEVLAETTVEVGNPFEADPIEVLIAVALRARRAFLR
                     HYKITPYMAAIPRSDELSAGGIALFANVFERLGVSGPAAGDAFHYYVSYCFGNALFAA
                     SRLNANEALGPEHPGHEVLESFREEAGVTGTSDESTRAAMDAVLDLSEMDPKRDEELF
                     LVGARRILESFADS"
     gene            1430..2155
                     /locus_tag="PLJ64_10315"
     CDS             1430..2155
                     /locus_tag="PLJ64_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HNC93919.1"
                     /translation="METTMNNALNRMKSLQPATLIALMALFVAVGGTATAASGLINGK
                     NIKPRTITSKQLKRGAVGTRQIRNKAISKTKLKPALLKSLKKTGATGATGAQGVPGEQ
                     GIPGPQGPKGATGAKGATGPKGAQGPAGIVTPQHAGNFNNQNIKGANVPMVTDNVPAR
                     SYVVMAKVAATATAASGLDCQLKAGDFVIDRSSTEFDAMFDQSFVYVQGVAPAGTTQV
                     RMLCSTNVETQINNRSIISIPVG"
     gene            complement(2152..3360)
                     /locus_tag="PLJ64_10320"
     CDS             complement(2152..3360)
                     /locus_tag="PLJ64_10320"
                     /inference="COORDINATES: protein motif:HMM:NF024945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC93920.1"
                     /translation="MSEAIVINFLIRTARQEPWRGLNPEVADVIEPELSGIADEILES
                     IGQLIPEYAMPLEGSFGKAVYRGVGEALDQFLVLIRDPDADRRASRKVYSILGRGELR
                     QGRSLDSLQSAYRIGARIAWRRIAAASLEAGLDGKTLSLLAESIFVYIDELSADSTEG
                     YAEAQFKQQGARLRRERGLLTLMLRDPAPDLSEIEQAARAAAWEIPKSLAAVACHERD
                     LAEVTRILPIGVISATVDGYGCIALPDPAGPGRAKQAARTFSGRTAVLGPVISPTRLA
                     ESWQLARTGFEASEAGALPADRLVRAEDHLVDLALFRSRDLIDRLRDQVMAPIGGLTP
                     KAHDRMAETALAYVEHSGNAAEMARSLNIHPQTARYRIARLKELFGDDLDRPDRRLEL
                     EISLRTRSFA"
     gene            3411..4358
                     /locus_tag="PLJ64_10325"
     CDS             3411..4358
                     /locus_tag="PLJ64_10325"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNC93921.1"
                     /translation="MTQHKMPLVDQIVNLFAVVIPFLATIAAIVLFWNQIVTPLDLAL
                     LAGMYLLTTFGITIGFHRLLTHRSFQTFKPIEYFLAICGSMAVEGPVNTWVADHRKHH
                     AHTDEEGDPHSPHVGHGSGVKGVLKGLFHSHIGWTFTDHGKAEQQKYAKDLYEDRGMR
                     FISDFFVLWVVLGLLIPAAIGYLATGTLAGAAGGFLWGGLVRVFFVHHITFSINSVCH
                     FLGSRRFEVDDHSTNVFWLALPSMGEAWHHNHHAFPRSAKHGLKKWELDPSAWVITAM
                     EKTHLAHNVIRITPERQAEKLAKPEPAGTGSGVPASDPR"
     gene            complement(4443..>5220)
                     /locus_tag="PLJ64_10330"
     CDS             complement(4443..>5220)
                     /locus_tag="PLJ64_10330"
                     /inference="COORDINATES: protein motif:HMM:NF016818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="siderophore-interacting protein"
                     /protein_id="HNC93922.1"
                     /translation="MVRIVLQSEGLRGFPIGEFTDHYVKLQLPPPGATYSPPFDPVRI
                     RESLAKDQWHRQRTYTVRGADPDAGEITIDFVTHGSGLAGPWARDARPGDPVQLVGPG
                     GAYTPDPDADWHLLAGDDAAIPAIAVALRRIPAGIPAVVVLEVENEAEQLSLVETEGL
                     TTEGELQLIWLHRDGAEHTAEPRILNVIEQLELPGGRGQAFVHGEAGMVREVRRHLVK
                     DRGMELEDLSATGYWKFRRTEEGWREDKPEWKRLAEADVS"
BASE COUNT          945 a         1718 c         1667 g          890 t
ORIGIN      
        1 agtttttcgt ccggtttgac ctcgttgacc acattccagc gctccagggt ctccgcgtcg
       61 aagagtccgc cggtcatcac cagttgccgg gcccgtcccg agcccgccct ttcggccagc
      121 ctttgaggtc cgcccatcga cggggtcagt ccgaccacga tctcgaccag gccgaatttc
      181 gccgactccg aggcgatgat caggtcgcag gcaagcgaga gctcgaaagc ggcggtcaag
      241 gtgagtccgt gagcggcgaa gacgaccggg aagggaaagc cctcgacccg gtcgatcagt
      301 ccgagcagct gctcccagag tcgcgtgcct tcggccgcgg tcagtccctg gaactcgttc
      361 acgttgacgc cgcctgagac gacgtccccc tcggcccgga tcaggagacc gcgcggcggg
      421 tcgtcctgga tcgcttcgaa ggcggccatc agctcttccg tcatggcccg gtcaaagagg
      481 tttagcggcg gactgttcag cgtgatcacg gccatcggac cgtcacgctc ggtggtaacg
      541 agtggcatgg tccggactct actcaggagt cggcgaagct ttcgaggatg cgcctcgctc
      601 cgaccaggaa aagttcctcg tcccgcttcg ggtccatctc cgacaggtcg agaacagcgt
      661 ccatggcggc ccgcgtcgat tcgtcggagg tgccggtgac cccggcctcc tcgcgaaagc
      721 tttcgagaac ctcatggccg ggatgctccg gtccgagcgc ctcgttcgca ttgagccggc
      781 tggccgcgaa cagcgcgttg ccgaagcagt agctcacgta gtagtgaaag gcatcgccgg
      841 cggccggtcc ggaaacaccg agccgctcga aaacatttgc aaagagcgca attccgccgg
      901 ccgacagttc gtccgaccgg ggaatcgcag ccatgtaggg ggtgatcttg tagtgccgga
      961 ggaaggcccg gcgggcgcgg agggcgacgg cgatcagcac ctcgatcggg tccgcctcga
     1021 acgggttgcc gacttcgacc gtggtttccg cgagcacctc ggtccagacc agttcgaccg
     1081 cagcctggac gatgtccgac ctcgagtcga agtgatggta gatcgcggac ggcgtgacat
     1141 tgagcgcagt ggcgagctgc cggatgctgg ggtcaccccc ctggtcgaag agacggagcg
     1201 tctcggctgc gatctcttcc ggctccagat gcgtactgcg acctgctgcc agtctggtca
     1261 atccaactct cctttgcctg cgcccgcatc gtgcacggac ggctgatccg gcaggaaccg
     1321 aacagcgcac ggaaactaac acgaaagcag cggggaaaac ttttccagcg gcagcgtaag
     1381 tttgtcgtgg cgggctcgtt gtctgcatag aagcaccgaa accgaccaca tggagaccac
     1441 aatgaacaac gcactcaacc ggatgaagtc gcttcagccc gccacgctta tcgccttgat
     1501 ggcgctcttc gtggccgtcg gaggaaccgc cacggccgcc tccggcctga tcaatggcaa
     1561 gaacatcaag cctcgaacga tcacctcgaa gcagctcaag cgcggtgccg taggtacccg
     1621 gcagatccgc aacaaggcga tcagcaagac aaagctgaag cccgcgctgc tcaagtccct
     1681 gaaaaagacc ggtgccaccg gcgcgaccgg tgcccagggc gtcccgggcg agcagggaat
     1741 ccccggtccg cagggaccga agggtgcaac cggcgccaag ggagcgaccg ggccgaaggg
     1801 cgcccagggg ccggccggaa tcgtcacccc gcagcacgcc ggcaacttca acaaccagaa
     1861 catcaagggc gcgaacgtcc cgatggtcac cgacaacgtg ccggcccgca gctacgtagt
     1921 gatggccaag gtcgccgcca ccgcgaccgc ggcgagcgga ctggactgcc agctcaaggc
     1981 cggggacttc gtgatcgacc gttcctcgac cgagttcgac gcgatgttcg accagagctt
     2041 cgtctacgtc cagggcgtcg ccccggcggg cacgacccag gtgcggatgc tctgctcgac
     2101 caacgtcgag acgcagatca acaaccgcag catcatctcg atcccggtcg gctaggcgaa
     2161 ggatcgggtg cgcagcgaga tctcgagctc gaggcggcga tctggtcggt ccagatcgtc
     2221 gccgaacagc tctttcagtc tcgcgatccg gtagcgcgcg gtctggggat gaatgttcag
     2281 gctgcgggcc atctcggcgg cgttgcccga atgttcgaca taggccaggg ccgtctccgc
     2341 catgcggtcg tgggccttgg gtgtgagacc gccgatcggc gccatcacct ggtcgcgcag
     2401 ccgatcgatc aggtcgcggc tgcgaaagag ggcaaggtcg accaggtggt cttcagccct
     2461 gaccagccgg tcggcgggaa gcgctcccgc ttcggatgcc tcaaatccgg ttcgcgccag
     2521 ctgccaggac tcggcaaggc gggtcgggct gatcacaggg ccgagcaccg cggttcggcc
     2581 ggagaaggtc cgggccgcct gtttggctcg cccggggccg gccgggtcgg ggagggcgat
     2641 gcagccgtaa ccgtcgaccg tggccgagat cacaccgatg gggaggattc gggtgacctc
     2701 ggccaggtcg cgttcatggc aggcaaccgc cgccaggctt tttgggatct cccacgccgc
     2761 cgcccgggca gcctgttcga tctcggaaag gtcgggtgcg gggtcgcgga gcatcagggt
     2821 gagcagtccc cgttctctcc tcagccgcgc gccctgctgc ttgaactggg cttccgcata
     2881 accctcggtc gaatcggccg agagctcgtc gatgtagacg aaaatcgatt cggccagcag
     2941 actgagcgtc ttgccgtcga gacccgcttc gagactcgct gctgcgatgc gccgccaggc
     3001 gatgcgggcc ccgatccggt aggcggactg aagtgaatcg agcgagcggc cctgacgcag
     3061 ctcgccgcgg ccgaggatcg aatacacctt gcggctggcg cggcggtcgg cgtcggggtc
     3121 gcggatcagg accaggaact gatcaagtgc ctcgcccact ccccggtaca ccgccttgcc
     3181 gaacgaaccc tcgagcggca tcgcgtactc gggaatcagc tggccgattg attcgaggat
     3241 ctcgtcggcg atcccggaga gctccggctc gatcacatcg gcgacctcgg gattgaggcc
     3301 gcgccagggt tcttgtcgcg cagttcttat aagaaagttt ataacaattg cttcagacac
     3361 ctttgattca gggaaagaaa tgcgcgaatt atcagcatat atttcacctc atgacccagc
     3421 acaagatgcc cctggtggac cagatcgtga acctgttcgc tgtggtcatt ccgttcctgg
     3481 ccacgatcgc cgcgatcgtc ctcttctgga accagatcgt caccccgctc gacctggctt
     3541 tgctggccgg gatgtacctg ctgacgacct tcgggatcac gatcggcttt caccggctgc
     3601 tgacccaccg ctccttccag accttcaagc cgatcgagta cttcctcgcg atctgcggtt
     3661 cgatggcggt ggagggcccg gtcaacacct gggtggccga ccaccgcaag caccacgccc
     3721 acaccgacga agagggcgac cctcacagcc cgcacgtcgg tcacggaagc ggcgtcaagg
     3781 gagtgttgaa gggactattc cactcccaca tcggctggac cttcaccgat cacggcaagg
     3841 ccgaacagca gaagtacgcg aaggatctct acgaagaccg cgggatgagg ttcatcagtg
     3901 acttcttcgt gctctgggtc gtcctcggtc tgctgatccc ggcggcaatc ggctatctgg
     3961 ccaccggcac gctggccggc gcggccggcg gtttcctatg gggcggactg gtgcgggtgt
     4021 tcttcgttca ccacatcacc ttttcgatca actcggtgtg ccactttctc ggcagccggc
     4081 gcttcgaagt cgacgaccat tcgaccaatg tcttctggct ggccctcccc tcgatgggcg
     4141 aggcgtggca ccacaaccat catgccttcc cccgctcggc aaagcacggc ctgaagaaat
     4201 gggaactgga ccccagcgcc tgggtaataa ccgcaatgga aaaaacccac ctcgcccaca
     4261 acgtcatccg cataaccccg gaacgccagg ccgaaaaact cgccaaaccc gaaccggcgg
     4321 ggacggggtc cggcgtaccg gcttccgacc cccggtaaac aggtcggttc gggcagtgcg
     4381 ccccgacgac gtttcgggcc gaggaggggt gtactgcccg gcccgaccct aaacgcccat
     4441 ggtcaactaa cgtcggcctc ggccaaccgt ttccattccg gcttgtcctc gcgccacccc
     4501 tcttcggtcc gccggaactt ccagtaaccg gtcgccgaaa ggtcttcgag ttccatgccc
     4561 cggtccttga ccaggtgcct ccggacctcg cgcaccatgc cggcctcgcc gtggacgaag
     4621 gcctggcccc ggccgcccgg cagttcgagc tgctcgatca cgttgaggat ccgcggttcg
     4681 gccgtgtgtt cggcaccgtc gcggtggagc cagatgagct ggagctcacc ttccgtggtg
     4741 agaccttctg tttcgaccag cgatagctgc tcggcttcgt tctcgacctc cagtaccacc
     4801 acggccggga tcccggccgg aatcctccgc agggcgacag cgatcgccgg aatcgcggca
     4861 tcatcaccag ccagaaggtg ccagtcggca tcgggatcgg gtgtgtaggc gcctccgggc
     4921 ccgaccagct ggacgggatc accgggcctg gcgtcgcggg cccaggggcc ggccagcccc
     4981 gatccgtggg tcacgaagtc gatcgttatc tcgccggcat cggggtcggc gccgcgaacg
     5041 gtgtaggtcc gctggcggtg ccattgatcc ttcgccaggc tctcccggat ccggaccggg
     5101 tcgaagggcg gtgagtaggt ggcaccggga ggcgggagct gaagcttcac gtagtggtct
     5161 gtgaattcac cgatcgggaa tccccggagc ccctccgact ggaggactat gcgaaccatg
//
LOCUS       DAOQKN010000173         5110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_8853_length_5110_cov_6.748368, whole genome shotgun sequence.
ACCESSION   DAOQKN010000173 DAOQKN010000000
VERSION     DAOQKN010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5110
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8853_length_5110_cov_6.748368"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="PLJ64_10335"
     CDS             complement(<1..294)
                     /locus_tag="PLJ64_10335"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNC93923.1"
                     /translation="MSPQTDGIEHGPLEDWFRERVAELEPPLDYEKIHGGMSNLTYLV
                     TDQGGSRWVLRRPPLGKVLGSAHDMGREVRVVRALAGTGVPVPPVVGYCEDEAV"
     gene            346..1407
                     /locus_tag="PLJ64_10340"
     CDS             346..1407
                     /locus_tag="PLJ64_10340"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNC93924.1"
                     /translation="MAMSARDDKEELALRKRTLDSLPSAVITVDSDGIVVCVNRTAEE
                     MVPTIGPGVKLRASLEELTNVEKVDRILFHREIATFAARPEGPELHWMLWDASEPEGE
                     FVLTVWDTDWSEVINERRVAFTMAASHELRGPLTTIRGFSEILNMQPESLSPEQAEAA
                     RIVEDSARLLSVLVDDIFDLTRSSFGELRLDLHEIDLNQVIAVVISSLEPGISDRGQT
                     LTLDAEELPVIEADESRMIQMISNLVSNASVHNAAGVKIRVKARRSGNEIAITVADDG
                     VGLPFDDPEAVFLSFRRGEAASDGDRTGSGIGVPLTKLLVELHRGRITVETGRGEGTS
                     FTLWLPVDREAALAEGTGL"
     gene            1411..2472
                     /locus_tag="PLJ64_10345"
     CDS             1411..2472
                     /locus_tag="PLJ64_10345"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNC93925.1"
                     /translation="MGPEPRKPSVAYLEQVLGVLPMVIAAIDDAGRIIWANAPTSRIA
                     PLLLETRSIREALAGIAHEQKLDRLLIRHEVITCPGRTGGPDLHWMVWTEPLAGGEWV
                     LMVWEADWSEEMNERRTAFTMAASHELRTPLTALLGFAEILEMDTDNLKPAQIEAVQM
                     ISQTALHLTKLVDDVFELTSNSFGELRLNLERVDLGEIARVLVEAHLQEAANRGDTLT
                     LEVQEDIPPVDADPARVRQMFANLVRNAMVHNPSGTRIVMKLWTDDDAVAVSVSDDGR
                     GLPFSNPKQALSSFSQGDGGSGDRTGAGIGLTIAKRLIELHRGSIEVQSTFGEGSTFT
                     LRFPIDRAKAISPGEPGPA"
     gene            2472..2834
                     /locus_tag="PLJ64_10350"
     CDS             2472..2834
                     /locus_tag="PLJ64_10350"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC93926.1"
                     /translation="MGRILVVEDSSAIRLLIRRRLEMAGHQVEEAANGLEALDQLGST
                     AEPDLIVLDESMPVFDDAGLLTRLREQWSELPVIVVSASPASESDPIMHSADARLAKP
                     INFERLFEVIDRLTSESP"
     gene            complement(2851..4410)
                     /locus_tag="PLJ64_10355"
     CDS             complement(2851..4410)
                     /locus_tag="PLJ64_10355"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNC93927.1"
                     /translation="MTEQEQTEEKLFKDLGLSPEIQQAIDEIGFDKPSPIQAEAIPEL
                     LAGHDVIGQAQTGTGKTAAFGLPMLQYLDPANDEVQAIVLTPTRELCIQVTQALRSYA
                     EHLDIEIVAVFGGAPIRSQQAQLRSGAHVVVATVGRMKDLISRRSLVLTAARFVVLDE
                     ADEMLDLGFIEDVERILRMCPSGRQTALFSATMPPPIKRLADTFMYDPVTISITPKTL
                     TVDAIEQAFVEVPGRAKVDKLIEVVKEEDPEQAILFCRTKIGASRLERDLKNRGLDVK
                     ALHGDLSQGVRDGVMLAFKNHRCKLLVATDIAARGLDVDHVTHVINYDIPNSSETYVH
                     RIGRTGRAGRTGRAITFVTPDQRRDLEQIKKDVKASISEWEKPEDRIEHARPPRRKRR
                     GEQPAPLDDQPAVAVEEVEEFDEVVEVPLEGPGGDQPETASDDIPDAREGAVKLFVNR
                     GSRSDITEEDLRWALREGAVLSDDQISDVRVLERFSFVEVDGDVAEKTVEFLDGTKLK
                     GSGIRVEIARS"
     gene            complement(4407..>5110)
                     /locus_tag="PLJ64_10360"
     CDS             complement(4407..>5110)
                     /locus_tag="PLJ64_10360"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNC93928.1"
                     /translation="SYAEKIAVPESSLVEIPDEIDDEQAAGIMIQGVTADGVLTISGN
                     LKPGETVVVGAAAGGTGSLAVQLARSMGAGKVIGLASGEEKQRLVLDLGADAVVDSRS
                     ERLAEEVTAAAGGQVDVVLEMAGGKSFDQLIETLRPFGRMVVVGIATREQNELRTGRL
                     LKNSLSVTGFWLMHLLARPDLARDSIDRVFGATARGDLKAVVGGTYGLSEVRRVHQDI
                     AGRKTVGKLLLDPTR"
BASE COUNT          893 a         1658 c         1640 g          919 t
ORIGIN      
        1 caccgcctcg tcctcgcagt agccgaccac cggaggcacc ggcaccccgg ttccggccag
       61 cgccctgacg accctcactt cgcggcccat gtcgtgggcc gagccgagaa ccttgcccag
      121 cgggggacgg cggagaaccc acctcgaccc gccctggtcg gtgaccagat aggtgaggtt
      181 cgacatgccg ccgtggatct tctcgtaatc gaggggaggc tcgagttcgg caacccgttc
      241 ccggaaccag tcttccagcg gaccgtgctc aatgccatcg gtttgaggac tcatggcaca
      301 agagattacg agtcagtgca ccgggatggc ggctaggctc tccccatggc aatgagtgcg
      361 agggacgaca aggaagagct ggccctccgc aagcgcacgc tcgattcttt gccaagcgcg
      421 gtgatcaccg tcgacagcga cggcatcgtg gtttgcgtca acaggaccgc ggaagagatg
      481 gtccccacga tcggtcccgg cgtgaagctc agggcttcgc tggaagagct gaccaacgtc
      541 gagaaggttg accggattct ctttcaccgc gagatcgcga ctttcgccgc ccggccggag
      601 gggcccgaac tccactggat gctctgggac gcttccgaac cggaggggga attcgtgctg
      661 acggtctggg acaccgactg gagcgaagtg atcaacgaac gccgcgtcgc attcacgatg
      721 gccgcctcgc atgaacttcg cggtcccctg accacaattc gcgggttctc ggagattctc
      781 aacatgcagc ccgagtcgct tagtccggaa caggccgagg cggcccgcat cgtcgaggat
      841 tccgcccgtc tcctgtcggt tctggtcgat gacatcttcg atttgaccag gagcagtttc
      901 ggagagctgc gtctggatct gcatgagatc gacctcaacc aggtaatcgc ggtagtcatc
      961 agctcactcg agcccggaat cagcgaccgc ggacagacgc taactctgga tgccgaagaa
     1021 ctcccggtga tcgaagccga cgagagccgg atgatccaga tgatttcgaa tctggtcagc
     1081 aacgcatcgg ttcacaatgc ggccggggtg aaaatccggg tcaaggcccg acggtccggg
     1141 aacgagatcg cgatcaccgt ggccgatgac ggcgtcggcc ttccattcga tgaccccgag
     1201 gctgtttttc tcagtttccg ccggggcgaa gccgcaagtg acggagaccg aaccggctcc
     1261 gggatcggag ttcccctgac caagcttctg gtcgaacttc atcgaggccg gatcacggtc
     1321 gagaccggcc ggggcgaagg cacctcgttc acgctgtggc taccggtcga ccgtgaagcg
     1381 gccctggccg agggaaccgg actctgaccg atgggtccgg aaccccggaa gccttcggtc
     1441 gcatacctgg agcaggtgct cggagtcctg ccgatggtga tcgccgcgat cgacgatgcc
     1501 ggccggatca tctgggccaa cgctccgact tcacgaatcg cacccctcct gctggagaca
     1561 cggtcgattc gggaagccct ggcgggcatt gcccacgagc agaagctcga ccgcctgctg
     1621 atccggcatg aagtgatcac ctgccccggc cggacgggtg gacccgacct ccactggatg
     1681 gtctggaccg agccactggc cgggggcgag tgggtcctga tggtctggga ggccgactgg
     1741 tcagaggaga tgaacgagcg acgcacggcg ttcaccatgg cggcctcgca tgagctcagg
     1801 acgccgctga ccgctctgct ggggttcgcc gaaatcctcg agatggatac tgacaacctg
     1861 aagcccgccc agatcgaagc ggtccagatg atttcccaga ccgcccttca tctgaccaag
     1921 ctggtcgatg acgtcttcga actgaccagc aacagcttcg gtgaactcag gctcaacctg
     1981 gagcgggtcg acctcggaga gatcgcccgg gttctggtcg aggcgcacct gcaggaagct
     2041 gcgaaccggg gcgacaccct gaccctggaa gtccaggaag atattccccc ggttgacgcc
     2101 gacccggcgc gggtccggca gatgttcgcc aacctcgtgc gtaacgcgat ggtccataac
     2161 ccctccggaa ccaggatcgt gatgaagctg tggaccgatg acgacgcggt agcggtctcg
     2221 gtttccgatg atggccgggg gctccccttc agcaacccga aacaggctct ctcaagcttc
     2281 agccagggag acggcggcag cggagaccgg accggcgccg ggattggcct gaccatcgcc
     2341 aagcgcctga tcgaactgca ccgcgggtcg atcgaggtcc agtcgacttt cggtgaaggc
     2401 agcaccttca ccctgcgttt cccgatcgat cgagcgaagg cgatttcgcc gggcgagccg
     2461 ggaccggcct gatgggccgg attctggtgg tcgaggattc atcggcgatc cggcttctga
     2521 tcaggcgaag actggagatg gccggacatc aggtcgaaga ggccgcgaac ggccttgagg
     2581 cgctcgatca gctcggatcc accgcagagc cggacctgat cgtgcttgac gagagcatgc
     2641 cggtgttcga tgatgccggt ttgctcaccc ggctcaggga acagtggtcc gagctgccgg
     2701 tgatcgtcgt cagcgcgagt cccgcctcgg aaagcgatcc gatcatgcat tcggccgatg
     2761 cccggctggc caagccaatc aatttcgaac ggcttttcga agtaatcgat cgcctgacca
     2821 gcgaatctcc gtgagccgct gtaggtggtc ctaactgcgg gcgatctcga cccggatgcc
     2881 tgaacccttg agcttggtgc cgtcgaggaa ctcgaccgtc ttctcggcca cgtcgccgtc
     2941 cacctcgacg aaggaaaagc gttccagcac ccgcacgtcg gagatctggt catcactgag
     3001 gacagcgcct tccctcaggg cccagcgcag gtcttcctcg gtgatatccg agcgggaacc
     3061 ccggttgacg aagagcttga ccgcaccctc cctcgcgtcc gggatgtcat cggaagcggt
     3121 ttccggctgg tcgccgcctg gaccctcgag cggaacctcg accacctcgt cgaattcctc
     3181 gacctcctcg accgccactg ccggttggtc gtcaagcgga gccggctgct cgccgcgacg
     3241 cttgcggcgg ggcggccggg cgtgctcgat ccggtcctcg ggcttctccc attcggagat
     3301 cgacgccttc acgtccttct tgatctgctc caggtcccgt cgctggtccg gggtgacgaa
     3361 ggtgatcgcc cggccggtac gccccgcccg gccggtacgc ccgatccggt gaacgtaggt
     3421 ctcggaactg ttcgggatgt cgtagttgat cacgtgcgtg acgtggtcga catccagccc
     3481 ccgggccgcg atgtcggtcg cgaccagaag cttgcagcga tggttcttga aggccagcat
     3541 cactccgtcg cggactccct ggctgaggtc gccgtgcagc gccttgacgt ccaggccgcg
     3601 gttcttcagg tcacgctcca gccgcgaagc cccgatcttg gtgcggcaga agaggatcgc
     3661 ctgctcggga tcctcttctt tgacaacctc aatcagcttg tcgaccttgg cccgccccgg
     3721 cacctcgacg aaggcctgct cgattgcgtc gacggtgagg gtcttcgggg tgatcgagat
     3781 ggtcaccggg tcgtacatga aggtgtcggc cagccgcttg atcggcggcg gcatggtggc
     3841 cgagaatagg gcggtctgac ggccgctcgg gcacatccgg aggatccgct cgacgtcctc
     3901 gatgaaaccg aggtcgagca tctcgtcggc ctcgtcaagc accacgaagc gggccgcggt
     3961 gaggaccagg gaccggcggg aaatcaggtc cttcatccgc cccacggtcg ctacgaccac
     4021 atgcgccccg gagcggagct gagcctgctg gctgcggatc ggcgccccgc cgaagaccgc
     4081 gacgatctcg atgtcgagat gctcggcgta ggaacggagc gcctgggtca cctggatgca
     4141 gagctcacgg gtcggagtga ggacgattgc ctgcacctcg tcgttggccg ggtcgaggta
     4201 ctggagcatg ggcagcccga aggccgcggt cttgccggta ccggtctggg cctggccgat
     4261 cacgtcgtgc cccgcgagga gctccgggat ggcttcggcc tggatcgggc tcggcttgtc
     4321 aaagccgatc tcgtcgatcg cctgctggat ctcgggcgag aggcccaggt ccttgaaaag
     4381 tttctcttct gtctgttctt gttctgtcat ctggttgggt ccagcagcag cttgccgact
     4441 gtcttccgtc cggcaatgtc ctgatggacc cggcggactt cggaaagccc gtaggttccg
     4501 ccgaccactg ccttcagatc tccccgggcg gtagcgccaa aaacccggtc gatcgagtcg
     4561 cgggcgagat cagggcgggc cagcaggtgc atcagccaga agccggtaac actgaggctg
     4621 ttcttcagca gtctgccagt cctgagctca ttctgctccc gggtggcgat tccgaccacc
     4681 accatgcggc caaacgggcg cagggtttcg atcaactggt cgaaggattt gccgcccgcc
     4741 atctccagca ccacatcgac ctggccgccg gcggccgcgg tcacctcttc ggccaggcgc
     4801 tcgctgcgcg agtcgacgac ggcgtccgcg ccgaggtcta gaaccagccg ctgtttctcc
     4861 tcccccgagg ccagcccgat cacttttccg gcgcccatcg agcgggccag ctggaccgcg
     4921 aggctgccgg tcccgccggc ggccgcaccg accaccactg tctcgcccgg cttcaggttg
     4981 ccggagatgg tcagtacccc gtcggcggtg actccctgga tcatgattcc ggcggcctgc
     5041 tcgtcatcga tttcatcggg gatctccacc agcgaacttt ccggcaccgc gatcttctcg
     5101 gcgtagctgc
//
LOCUS       DAOQKN010000174         5093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_8905_length_5093_cov_5.962882, whole genome shotgun sequence.
ACCESSION   DAOQKN010000174 DAOQKN010000000
VERSION     DAOQKN010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5093
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8905_length_5093_cov_5.962882"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1204
                     /locus_tag="PLJ64_10365"
     CDS             <1..1204
                     /locus_tag="PLJ64_10365"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HNC93929.1"
                     /translation="HDIAFLSRIRDGVKGFEVRVGGGTSIMPRVAPTIYDFVEAENGD
                     YLKHAEAILRVYDRQAWLRPNRARARIKVLVDKVGIEEVRRMVDEELKGDWVNERDFT
                     IDHLVFDVDEEASLTEPLPPSGSPNGDRAEFDRYLEANVQPQRQEGFHTVEVKVVRGD
                     LTPEQFRGLGQIMRDYTGGFARMTVQQNIVLRWVREESLYDVWKRLAELGLDGVGPRR
                     ITDVVSCPGTDSCKMGITSSMGLNQAIQERVESMDITDPLTRDIHIKMSGCPNGCSQH
                     HIANIGFYGASLKVGDKTIPAYVPHLAGKYTDGDTIYGTRLKSRLPARRVPDAVERWI
                     RLYETDREEGEIFNAYVERVGTARMEEEVKDLTMPVDFSLDTMQMFIDWQRSDPYKVE
                     RGEGECAV"
     gene            1214..1969
                     /locus_tag="PLJ64_10370"
     CDS             1214..1969
                     /locus_tag="PLJ64_10370"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HNC93930.1"
                     /translation="MEPSSTLTPAEAEAARKAEEATRVLAARFDAEAEATRVEPLDAS
                     QLLEWAFEQFGQDMYIACSFQKTSSVVMHLATEINPEARFFYIDTDFLFPETYETRDR
                     LAEHFGVEFERWHNISPEEQAREFGDQLWKRDPDACCGIRKVDPMARALASGVDCWVA
                     GIRREDSATRAGSPKFMWDRKFNIWKLNPIADWSERDVWTYLHKHGIPYNPLHDQGYP
                     SIGCTHCTRPVLPGQSSRDGRWSGSGKTECGIN"
     gene            2063..3130
                     /gene="sat"
                     /locus_tag="PLJ64_10375"
     CDS             2063..3130
                     /gene="sat"
                     /locus_tag="PLJ64_10375"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF003166.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HNC93931.1"
                     /translation="MTELALQLSERQTADFEMIGNGGFAPLTGFQGSADWQRVCDEMR
                     TADGDYWPIPITLATDLECEVGDVVSLTSDEGKALGRITIEEIFERDVEHEAKSVYQT
                     NDQEHPGVAAIREEGARCLAGPIEVDALPDHEEAFQRRYLTPAESKQAFADRGWKRVV
                     AFQTRNPIHRAHEYLTKAALEVSDGLFIHPLIGKTKAGDIPADVRMKCYELLMEDYYP
                     NDRVILGVNPSKMHYAGPREAVLHAIVRRNYGCTHFIVGRDHAGVGDYYGTYDAQKIF
                     EGIDIDELGIEPLMFEHTFWCNECEGLASNKTCPHDAEAHLFLSGTKVREMLGNGEMP
                     PPEFSRPEVAQILIDAYKEDN"
     gene            3134..3685
                     /gene="cysC"
                     /locus_tag="PLJ64_10380"
     CDS             3134..3685
                     /gene="cysC"
                     /locus_tag="PLJ64_10380"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF002059.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HNC93932.1"
                     /translation="MSDSKGFTLWFTGLSGAGKTTISEIVARELTDRGSKLEILDGDI
                     VRENLSKGLGFSKEDRDINIRRIAFVADLLSRNGVPVITAAISPYRALRDEARELMGD
                     RFIEVYIEASVEVCAERDVKGLYAKAFSGEIKEFTGVSDPYEAPENPEIALKTAEEEP
                     EESAARLLAYLEERQLIPAVVAS"
     gene            3784..4257
                     /locus_tag="PLJ64_10385"
     CDS             3784..4257
                     /locus_tag="PLJ64_10385"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNC93933.1"
                     /translation="MSVFLTIERAEAPAGHEPSRVPLVDADGQITIDPWWGTIQPQRL
                     HPGIPTWGELEVIAHIEAGGRLIDTRLPEYASASGTIPGSLVLPWETITDHLEVFDPA
                     SVTVLFCNGPQCAATPRAVGRLLDAGVEPESLAYYRGGIQDWVGLGLPTAPVSGG"
     gene            complement(4379..>5093)
                     /locus_tag="PLJ64_10390"
     CDS             complement(4379..>5093)
                     /locus_tag="PLJ64_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93934.1"
                     /translation="LTDHDGVAGVPEAVEAGAQLGVEIVPAVEMSSVHEYAEDLHICG
                     YWVDVEKITPACQRAQEERRNRAKEIVQNLRDHGFDLTFEDAVREAGAADAIGRPHIA
                     KAAGATGNLGPFFEEWLVPGAKAFVSRKWPDANEACALIREAGGVPVIAHPYWDIKVP
                     EQVEELIRALDIGGIEVFYPSHTRAQTKHLLDLANELGVTATASSDYHGPTHKTFSRF
                     GAYQTYDLGQPEVPPRPAP"
BASE COUNT          912 a         1739 c         1614 g          828 t
ORIGIN      
        1 ccatgacatc gccttcctca gccggatcag ggacggcgtc aagggcttcg aggtccgggt
       61 cggcggcggc acctcgatca tgccccgggt cgcccccacg atctacgact tcgtcgaggc
      121 cgaaaacggc gattatctca agcacgccga agcgatcctg cgggtttacg accgccaggc
      181 ctggctgcgt ccgaaccggg cccgcgcccg gatcaaggtc ctggtcgaca aggtcgggat
      241 cgaagaggtc cgccgcatgg tcgacgagga actgaagggc gactgggtga acgagcgtga
      301 tttcacgatc gaccacctgg tcttcgacgt cgacgaggaa gcctcgctga ccgagcccct
      361 gcccccgagc ggctcgccga acggcgaccg cgccgaattc gaccgctacc tcgaagcgaa
      421 cgtccagccg cagcgccagg agggcttcca cacggtcgag gtcaaggtgg tccgtggcga
      481 cctcaccccg gagcagttcc gcggccttgg ccagatcatg cgggactaca ccggcggctt
      541 cgcccggatg accgtccagc agaacatcgt cctgcgctgg gttcgcgagg aatccctcta
      601 cgacgtctgg aagcggctcg ccgaactcgg gctcgatggc gtcggcccgc gccgcatcac
      661 cgacgtagtc tcctgccccg gcaccgactc ctgcaagatg ggcatcacct cctcgatggg
      721 cctcaaccag gcgatccagg aacgggtcga gtcgatggac atcaccgacc ccctgacccg
      781 cgacatccac atcaagatga gcggctgccc gaacggatgc agccagcatc acatcgccaa
      841 catcggcttc tacggcgcgt cgctgaaggt cggcgacaag accatcccgg cctacgtgcc
      901 gcacctcgcc ggcaagtaca ccgacgggga cacgatctac ggcacccgcc tcaagtcccg
      961 cctgcccgcc cgccgggttc cggatgcggt cgagcggtgg attcgcctct acgagaccga
     1021 tcgcgaggaa ggcgaaatct tcaacgcgta cgtcgagcgg gtcggcaccg cccgcatgga
     1081 agaagaagtg aaggacctga ccatgccggt cgacttcagc ctcgacacca tgcagatgtt
     1141 catcgactgg cagcgctccg acccgtacaa ggtcgagcgc ggcgaaggcg agtgcgccgt
     1201 ctaggaaacc ttgatggagc cgtccagcac actcactccg gcggaagccg aagcggcgcg
     1261 caaggccgag gaggccactc gcgtcctggc cgcgcgcttt gacgcggagg cggaggccac
     1321 ccgggtcgag ccgctggatg cgagccagct gctggagtgg gccttcgagc agttcgggca
     1381 ggacatgtac atcgcctgct ccttccagaa gaccagctcg gtggtgatgc acctcgccac
     1441 cgagatcaac cccgaagccc gcttcttcta catcgacacg gacttcctct tccccgagac
     1501 ctacgagacc cgagaccggc tggccgagca tttcggagtc gagttcgagc gctggcacaa
     1561 catcagcccc gaggagcagg cccgcgagtt cggtgaccag ctctggaagc gcgacccgga
     1621 cgcctgctgc ggcatccgca aggttgatcc gatggcccgg gcgctcgcat ccggagtcga
     1681 ttgctgggtc gccgggatcc ggcgcgagga ttcggcaacg cgggccggct cgccgaagtt
     1741 catgtgggac aggaagttca acatctggaa gctgaacccg atcgccgact ggtccgaacg
     1801 cgacgtctgg acctaccttc acaagcacgg aatcccctac aaccccctgc acgatcaggg
     1861 ttatccctcg atcggctgca cccactgcac ccgtcccgtt ttgcccggcc agtcttcccg
     1921 tgacgggcgc tggtccggct cgggcaagac ggagtgcggc atcaactagc cgctgtcgga
     1981 cggcatttgc cgcccataac ggctaggctg tccgattctt caccgttcct gtcaacattc
     2041 cctcctcctt atgaaccaaa ccatgactga acttgccctc cagctgtccg agcgtcaaac
     2101 cgcagacttc gaaatgatcg ggaacggggg tttcgcaccc ctgaccggct tccagggctc
     2161 ggcggactgg cagcgtgtct gcgacgaaat gcggaccgcc gacggtgact actggccgat
     2221 cccgatcacc ctggccaccg acctcgaatg tgaggttggc gacgtggtca gcctgacctc
     2281 ggacgaaggc aaggcgctcg gccggatcac gatcgaggag atcttcgagc gcgacgtcga
     2341 gcatgaagcg aagtcggtct accagaccaa cgaccaggag caccccgggg tcgctgcgat
     2401 ccgcgaggag ggcgcccgtt gcctcgccgg cccgatcgag gtcgacgccc tgcccgatca
     2461 cgaggaagcc ttccagcgcc gctacctgac cccggccgag tcgaagcagg ccttcgccga
     2521 ccgcggctgg aagcgggtcg tggctttcca gacccgcaac ccgattcacc gcgcccacga
     2581 gtacctgacc aaggcggcgc tcgaggtttc cgacggtctc ttcattcacc cgctgatcgg
     2641 caagaccaag gccggcgaca tcccggctga cgtgcggatg aagtgctacg agctgctgat
     2701 ggaggactac tacccgaacg accgggtgat cctcggggtc aacccctcga agatgcatta
     2761 cgccggcccc cgtgaggccg tgctccacgc gatcgtgcgt cgcaactatg gctgcaccca
     2821 cttcatcgtc ggccgcgacc acgccggcgt cggtgattac tacggcacct atgacgccca
     2881 gaagatcttc gaaggaatcg acatcgacga gctcgggatc gagccgctga tgttcgagca
     2941 caccttctgg tgcaacgaat gcgagggcct ggcttcaaac aagacctgcc ctcacgacgc
     3001 cgaagcgcac cttttccttt ccggcaccaa ggtccgcgag atgcttggca acggcgaaat
     3061 gccgccgccc gagttctcgc gccccgaggt cgcccagatc ttgattgacg cttacaagga
     3121 ggacaactaa ccaatgtcag acagcaaggg attcaccctc tggttcaccg gcctctccgg
     3181 tgccggcaag accaccattt ccgagatcgt cgccagggag ctgaccgacc gcggctccaa
     3241 gctcgagatt ctcgatggtg acatcgttcg cgaaaacctc tcgaagggcc tcggcttctc
     3301 gaaggaggat cgtgacatca acatccgccg gatcgccttc gtcgccgacc tgctttcccg
     3361 caacggcgtc ccggtgatca ctgccgcgat ctcgccctac cgggcactcc gcgacgaggc
     3421 tcgagagctg atgggtgacc gcttcatcga ggtctacatc gaggcgtcgg ttgaagtctg
     3481 cgccgaacgc gacgtgaagg gtctctacgc caaggccttc tccggcgaga tcaaggaatt
     3541 caccggggtc tccgacccgt acgaggcacc ggagaatccg gagatcgccc tcaagaccgc
     3601 ggaagaggag ccggaggaat cggctgccag gctgctcgcc tacctggaag agcgccagct
     3661 catcccggcg gtcgtcgcct cgtaggcaac cgcgaaaacg tacttcgccg gggcgggctt
     3721 tcgagcccgc cccttcttcc ctctataaac aactgtttag ttgtgtagtg atatactttg
     3781 gccatgtccg ttttccttac catcgaacgc gccgaggcgc cggccgggca cgagcccagc
     3841 cgggtcccgc tggtcgatgc cgacggacag atcacgatcg acccctggtg gggcacgatc
     3901 cagccgcagc ggctccaccc cgggattccc acctggggtg agctggaagt catcgcgcac
     3961 atcgaggcgg gcgggcgtct gatcgacacc cggctgcctg aatacgcatc ggcgagcggg
     4021 acgatccccg gctcgctggt tctgccctgg gagacgatca ccgaccacct ggaggtgttc
     4081 gatcccgcaa gcgtgacggt gctcttctgc aacgggccgc agtgtgcggc aacccccaga
     4141 gcggtgggaa ggctgctgga cgccggggtc gagcccgaat ctctcgccta ttaccgcggc
     4201 gggatccagg actgggtcgg gctcggcctc cccaccgccc ccgtttcggg cggctgaaag
     4261 cttccgggcg ggagagcccg gaccagagtc gcggtggagc cccgcccccg gtggctccat
     4321 ccgcggctgc ccggacatgg ggaagggccg gcgggaaggg atcccgcggg ggttcaggtc
     4381 agggcgccgg cctgggcggg acttcgggct ggcccaggtc gtaggtctgg taggcgccga
     4441 agcggctgaa ggtcttgtgg gtggggccgt ggtagtcgga tgaggcggtg gcggtcacgc
     4501 ccagctcgtt tgcgaggtcc aggaggtgct tggtctgggc cctggtatga gacgggtaga
     4561 agacctcgat gccaccgatg tcgagggcgc ggatcagctc ctcgacctgc tccggaacct
     4621 tgatgtccca gtaggggtgg gcgatcaccg gcacgccgcc ggcctcgcgg atcagggcgc
     4681 aggcctcgtt cgcgtccggc cacttgcggg agacgaacgc ctttgccccc ggcaccagcc
     4741 actcctcgaa gaaggggccg aggttgccgg tcgccccggc tgccttggcg atgtggggcc
     4801 ggccgatcgc gtcggcggcg cccgcctcgc ggaccgcatc ctcgaaggtc aggtcgaagc
     4861 cgtggtcgcg caggttctgg acgatctcct tcgccctgtt ccgccgctct tcctgggccc
     4921 gctgacaggc cggggtgatc ttctccacgt cgacccagta gccgcagatg tgaaggtcct
     4981 ccgcgtactc gtgaaccgag gacatctcga ccgccggcac gatctccact ccgagctgcg
     5041 ccccggcctc gaccgcctcc ggcacacccg ccaccccgtc gtgatcggtc agg
//
LOCUS       DAOQKN010000175         5003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_9169_length_5003_cov_6.367017, whole genome shotgun sequence.
ACCESSION   DAOQKN010000175 DAOQKN010000000
VERSION     DAOQKN010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 5003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5003
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9169_length_5003_cov_6.367017"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="PLJ64_10395"
     CDS             <1..289
                     /locus_tag="PLJ64_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HNC93935.1"
                     /translation="TGGNLLLLDEPTNDLDVDTLRALEDALLNFAGCAVVISHDRWFL
                     DRVATHILAFEGDSQVVWFEGNLEAYEENRRNRLGAEADRPHRITYKRLTR"
     gene            complement(369..740)
                     /locus_tag="PLJ64_10400"
     CDS             complement(369..740)
                     /locus_tag="PLJ64_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93936.1"
                     /translation="MKKLLIPMILALAVFCGTGSAAQANSGASASAACSSKGNFPPAS
                     YVTSIAARGVSCRGARRVVRAYHACRAANGGANGRCNHKVLGFRCVEGRRQSVPGVQY
                     SVGVSCSKGSNKVNSTYTQNV"
     gene            complement(791..1450)
                     /locus_tag="PLJ64_10405"
     CDS             complement(791..1450)
                     /locus_tag="PLJ64_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93937.1"
                     /translation="MEVASHGRALTGICLVVVLGSVSLIWATDARAGCGGVETAGAKR
                     VVNPGGHAPLAIGDSTMLLALPNLARAGYRVNARGCRGWEEGLEVLRTEKRHRRLPHL
                     VLMALGADWSVTVSQIRRTLHLLGPRRVLALMTPLELGGWAGDDARSMRRAFRRYPTR
                     IVLLDWVRYSRGHGEWFQPDGTHLTFSGANAFASLARRATPWARHGEMPDHNFFPKAG
                     H"
     gene            1549..2946
                     /locus_tag="PLJ64_10410"
     CDS             1549..2946
                     /locus_tag="PLJ64_10410"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HNC93938.1"
                     /translation="MLFPTVEFAIFFPVVLALSWLLMPRQKLWKPYIIIASYVFYAAA
                     NPKFCLLLAGITLGNQAAAKLIAAAGTERRRKWITGVAVGLDLALLGVFKYYAFFVQD
                     WADLLDSFHLGAPLPLLTIALPIGVSFFTFQAITYVVDVKRGDAEPASTTDVAIYLSF
                     FPHLVAGPIVRASEFLPQLKAPRDPNKVAVGAGLALVGLGVIKKVLIADYLARVLVDP
                     VFAVPQAYAAPDALLAAYGYAVQIYCDFSGYTDIAIGLALLMGFIFPQNFNRPYRATG
                     FRDFWRRWHMTLSRFLRDYLYIPLGGSRGSRLFTYRNLMITMILGGLWHGAAWTFVLW
                     GAFQGAALVTEHALDGRVGRRTPVWLRWFLTFNLVTFAWILFRSQNLGLFWDYLGRFF
                     VSGPATLYTLPVLLAIIVVIGMQLLPERPMERLQVRLEQLNPALLAVMLAATILVVGA
                     TVPSQGVPPFIYFQF"
     gene            2958..3983
                     /locus_tag="PLJ64_10415"
     CDS             2958..3983
                     /locus_tag="PLJ64_10415"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNC93939.1"
                     /translation="MTEHRDDSEMSRFDREGVRHFTARQSVLAVAIVAAILLVLSGGS
                     ILSAADEEKPGVEKDLLHAVGKPGSTLADNPLGRASAKLTSNLSPDEKLDPSQSFAQT
                     GTGAVTAGQIPPVTPSYFDPVELDDKAPPRQPLESLLVTGDSLSQPLDAELARRLTPE
                     GIEVTRDAHVGTGISNASLADWGQLSAAQVAEHHPDAVVVFIGANEGYPMPGPDGKDV
                     ACCSPEWAAIYANRARQVITNYRQGEKAKVYWLTVPLPRDPARQRIASVVNQAVAVAA
                     QPWRSQVRIVDTDEYFAPEGTYSDSIPVDGKDQIVRESDGIHLNDLGSSVLADHLLDR
                     IDLDFTR"
     gene            4054..4929
                     /locus_tag="PLJ64_10420"
     CDS             4054..4929
                     /locus_tag="PLJ64_10420"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNC93940.1"
                     /translation="MATFTRVLVGHDGGKHAEDALSLAKLIAERTGAELVLASVIPNP
                     VGGSFGPALPAEAFTELAGKARASLEAAAARVGAGVEVEQSSSAAHGLQAIAERIGAD
                     LIVVGSSPSAETGHTRAGRKARQLLSGADCAIAVAPDGFHDRAGLDRIGVGIDGSPES
                     RLATETAVGLAPDGSELQLLAVAAGFADEWGRWGVTYPLAEMAEATRESADALLKEME
                     QVVPARLTAKKLLLEGTAPIQLAEASAGLDLLCLGSRGYGPVRRVLLGSVSSDVMNHA
                     ASAVLVVPRTDQTND"
BASE COUNT          773 a         1759 c         1606 g          865 t
ORIGIN      
        1 gaccggcggc aacctgctgc tgctcgacga gccgaccaac gacctcgacg tggacacgct
       61 ccgcgccctc gaggatgcac tgctcaactt cgccggctgc gccgtggtga tctcgcacga
      121 ccgctggttc ctcgaccggg tcgcgaccca catcctcgcc ttcgagggcg attcccaggt
      181 ggtctggttc gaggggaacc tcgaggccta tgaagaaaac aggcggaacc gcctgggggc
      241 cgaggcggac cgcccgcatc gcatcactta caagcgactg acccgctgag cttcaaccgg
      301 gatggcagaa gtcagtcgta ttgactgact tctgccatct ggggttgccc ccggcggccg
      361 ggagtggctc agacgttttg cgtgtaggtc gagttgacct tgttgctgcc cttgctgcag
      421 gaaaccccga ccgagtactg gacgccgggc acggactgac gccggccttc gacgcagcgg
      481 aacccgagca ccttgtgatt gcagcggccg ttggcgcccc cgttggcggc gcggcaggcg
      541 tggtaggccc tgaccacccg gcgggcaccc cggcaggaaa caccgcgggc ggcgatgctg
      601 gtcacgtagg aggccggcgg gaaattgccc ttggaggagc aggcggccga ggccgacgcg
      661 ccggaattcg cctgcgcggc cgaaccggtc ccgcagaaca cggccagtgc caggatcatc
      721 gggattagaa gctttttcat cttttccttg cctttttcgt tgtttgttcc gaccggcgct
      781 ggtcctcgac tcagtgcccg gccttgggga agaagttgtg gtcgggcatc tcgccgtgcc
      841 ttgcccaggg agttgccctt cgggcgaggc tcgcaaacgc attggcgccg ctgaaggtca
      901 ggtgagttcc gtccggctgg aaccattcgc cgtggccccg gctgtagcgg acccagtcga
      961 gcaggacgat tcgggtcggg taacgacgaa aggcccgacg catgctccgc gcgtcatcac
     1021 cagcccagcc gcccagctcg agcggcgtca tcagggcgag caccctccgc gggcccagca
     1081 ggtgaagggt gcgccggatc tgggacacgg tgacgctcca gtcggcgccg agggccatca
     1141 gcaccagatg cgggagcctg cggtgccgct tttcggtgcg caacacctcg aggccttctt
     1201 cccatccccg gcatccgcgc gcgttgacgc gataaccggc ccgggcgaga ttcgggagcg
     1261 cgagcagcat cgtggaatcg ccgatcgcca gcggcgcgtg gccaccgggg ttcaccaccc
     1321 ttttggcgcc ggcggtctcg accccgccac aacccgcccg ggcgtcggtt gcccagatga
     1381 gcgacacgct ccccagcacg accacgaggc agatccccgt caaagctctc ccatgcgaag
     1441 cgacttccat gcacccgtga atttaccggt accggccccc tctggttgca ggtatcggaa
     1501 ataatccggt ttccccagcg tggattcagc taccggggac ctcgtccgat gctcttcccc
     1561 accgtcgaat tcgccatctt cttccccgtg gtcctggcgc tctcctggct gttgatgccg
     1621 cgccagaaac tctggaagcc gtacatcatc atcgccagct acgtcttcta cgcggcggcg
     1681 aaccccaagt tctgcctgct cctggccggc atcaccctcg gcaaccaggc cgccgcgaag
     1741 ctgatcgcgg ccgccggaac cgagcggcgc cgcaagtgga tcaccggagt ggcggtcggg
     1801 ctcgacctcg ccctgctcgg ggtgttcaag tactacgcct tcttcgtcca ggactgggcc
     1861 gacctactcg actccttcca cctcggggca ccgctgccgc tgctcaccat cgcgctgccg
     1921 atcggtgtca gcttcttcac cttccaggcg atcacctacg tggtggacgt caagcgcggt
     1981 gacgcggagc ccgcctcgac caccgacgtc gcgatctacc tcagcttctt tccgcatctc
     2041 gttgccgggc cgatcgtgcg ggccagcgag ttccttcccc agctaaaggc accgcgggat
     2101 ccgaacaagg tcgcggtcgg ggccggcctc gccctggtcg gcctcggcgt aatcaagaag
     2161 gtcctgatcg ccgactacct ggcccgggtc ctggtcgacc cggtcttcgc ggttccccag
     2221 gcctacgccg caccggacgc cctgctcgcg gcttacggct acgcggtgca gatctactgc
     2281 gacttctccg gctacaccga catcgcgatc gggctggccc tgctgatggg cttcatcttc
     2341 ccgcagaact tcaatcggcc ctaccgggcg accggcttcc gggacttctg gcgtcgctgg
     2401 cacatgaccc tctcccgctt cctgcgcgac tacctgtaca tcccgctcgg gggcagccgc
     2461 ggcagccggc tgttcaccta ccgcaacctg atgatcacga tgatcctcgg cggcctctgg
     2521 cacggcgcgg cctggacctt cgtgctctgg ggcgccttcc agggcgccgc cctggtcacc
     2581 gagcacgcac ttgacggccg ggtcgggcga cgcaccccgg tctggctgcg ctggtttctc
     2641 accttcaacc tggtcacctt cgcctggatc ctcttccgct cccagaacct cgggctcttc
     2701 tgggactacc tcgggcgatt cttcgtctcc gggccggcga cgctctacac cctgccggtg
     2761 ctcctcgcga tcatcgtggt gattggcatg cagcttctgc cggaacggcc gatggaacgg
     2821 ctccaggtcc ggctcgagca gctcaatccc gccctgctgg ccgtcatgct cgcggctacc
     2881 atccttgtcg tcggcgccac ggttcccagc cagggcgtgc cgccgttcat ctacttccag
     2941 ttctaggggt catcgccgtg actgaacatc gagacgacag tgaaatgagc cgcttcgacc
     3001 gcgagggcgt acggcacttc acggcccgcc agtcggtgct ggcggtggcg atcgtcgccg
     3061 cgattttgct ggtgctttcc ggcggctcga tcctcagcgc cgccgacgag gaaaagccgg
     3121 gtgtcgagaa ggacctgctc catgcggtcg gcaagcccgg gtcgaccctg gccgacaacc
     3181 cgctcggccg ggccagcgcc aagctgacct cgaacctctc tccggacgag aagctcgacc
     3241 cttcccagtc gttcgcccag accggaaccg gggcggtgac cgccgggcag atccctccgg
     3301 tcactccttc ctactttgat ccggtcgagc tcgatgacaa ggcgccgccg cggcagccgc
     3361 tggagagcct gctggttacc ggtgactctc tctcgcagcc gctcgacgcc gaactggcca
     3421 ggcggctgac cccggaagga atcgaggtga cccgggacgc tcacgtcggg accgggattt
     3481 ccaatgcatc gctggccgac tggggtcagc tttcggcggc ccaggtcgcc gagcaccacc
     3541 cggatgccgt ggtcgtgttc atcggcgcaa acgagggcta tccgatgccc ggaccggacg
     3601 gcaaggacgt tgcctgctgc tccccggagt gggccgcgat ctacgcgaac cgcgcccgcc
     3661 aggtgatcac caactaccgg cagggcgaga aggcgaaggt ctactggctg accgtgccgt
     3721 tgccgcggga tccggcccgc cagcggatcg cctcggtggt caaccaggcg gtagccgtgg
     3781 ccgcccagcc gtggcgttcg caggtccgca tcgtggacac cgacgagtat ttcgctccgg
     3841 aggggaccta cagcgattcg atcccggttg acggcaagga ccagatcgtc cgggagtcgg
     3901 acgggatcca cctcaacgac ctcggatcat ccgtcctcgc cgaccacctg ctcgaccgga
     3961 tcgacctcga cttcacccgc tgagccgcgg tcacttgccg gttccgtaga ttcccccctt
     4021 gcaagttcgg gccgggggcg gaatcattca gccatggcca cattcacccg tgtgctggtc
     4081 ggccacgacg gcggcaagca cgcagaagat gccctttccc tcgcgaagct gatcgccgaa
     4141 aggaccggcg ccgaactggt cctcgcctcg gtcattccga atccggtcgg tggcagtttc
     4201 ggtccggcgc tcccggccga ggccttcacc gagctggccg gcaaagccag ggccagcctc
     4261 gaggcggccg ctgccagggt tggtgccggc gttgaggtcg agcaatcctc ctcggcggca
     4321 catgggcttc aggcaatcgc cgagcggatc ggagcggacc tgatcgtggt cggttccagc
     4381 cccagcgcgg aaaccggcca tacccgggcc ggccgaaagg cgaggcagct cctttccggc
     4441 gccgactgcg cgattgctgt cgccccggac gggtttcacg accgggccgg tctcgatcga
     4501 atcggagtcg gcatcgacgg ttcgccggag tcaaggctgg cgaccgagac ggccgtcggg
     4561 ctcgcgccgg atggctcgga acttcagctg ctggcggtgg ccgccggttt cgccgacgaa
     4621 tggggtcgct ggggagtcac ctacccgctg gctgaaatgg cggaagcgac ccgcgagtcc
     4681 gccgacgctc tgctcaagga aatggaacag gtggttccgg cccgactcac cgcgaagaag
     4741 ctgctgcttg aaggaaccgc gccgatccag ctggccgagg cctcggccgg gcttgacctg
     4801 ctctgcctgg gctcgagggg ctacggaccg gtgcggcggg tgctgctcgg ctcggtctcc
     4861 tccgacgtga tgaaccacgc cgcctcggcg gttctggtcg taccccgaac cgaccaaacc
     4921 aacgactgac cccggcggcc ctgcgggcgg atgccagggg cctccagccg ttcgctggca
     4981 aggagacagg ccggaaatga ccg
//
LOCUS       DAOQKN010000176         4983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_9231_length_4983_cov_5.147727, whole genome shotgun sequence.
ACCESSION   DAOQKN010000176 DAOQKN010000000
VERSION     DAOQKN010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4983
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9231_length_4983_cov_5.147727"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="PLJ64_10425"
     CDS             complement(<1..219)
                     /locus_tag="PLJ64_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNC93941.1"
                     /translation="MNVNWDGEDLGESLPGRQGRLLFAYLALNRHRPVRRDELVEALW
                     AEEGLPSGGEALLAPPLSRLRKALGPGRL"
     gene            315..1613
                     /locus_tag="PLJ64_10430"
     CDS             315..1613
                     /locus_tag="PLJ64_10430"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC93942.1"
                     /translation="MSTPSLINLLPAAARMAPETPEEVASALVEAGRQSRKVVVFSTG
                     HGIPPIGDTSDRVLLDMSAFDKVEIDPLARTARIGGATQWMELISAANEHGLTAPFGS
                     AATVGVTGYMMGGGVGFMSRHLGLGSSAIRSAELVTPDGRIRRIDAGSDPDLFWALRG
                     GGGGFGVITELEIELTPVPRMAGGVLGWGIEDAERVLGAWSEWTRRIDDDVTSAVRFM
                     HPPEGPALTVVVVTAPRAAEELAAILKPLTDLKPMLNTVRDTDPEAFLREYTDPEIDP
                     AEPPHAFEHVLLDRLPPEAVQLTAEFAQPVTGCGAMMVEIRHMGGALGRPAPGGGASD
                     RIDGEYLFFVVGFPDVAETMNHAVDVLSDFGRGRTYFNFMPRRTGRETAYGQEAIARL
                     ADLYEAVDPEGLIDHPHPISRTASVADETATTQVLAILHG"
     gene            1721..>4983
                     /gene="mfd"
                     /locus_tag="PLJ64_10435"
     CDS             1721..>4983
                     /gene="mfd"
                     /locus_tag="PLJ64_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNC93943.1"
                     /translation="MTLRPTIDLLLEDEQISRLSAEVREAVAGSAGTTVRAEVSATLR
                     PALIAAMLEDDFGLEDRPALIVTPDDRSAREMAAALGAFLGDRMVRSYPSRGTGYASQ
                     VTPPPHLTGLRIDALDSFGREEAHPPVVVASAIALAESVPDASLRPAGFRLTVGEEHD
                     PSQVAADLVAAGYERVDQVQERGQFAVRGGILDVFSATEDRATRVDFFGDEIESMRWF
                     STFTQRSLGQAESIELSPAAELDAEHREMAELIIAEARENETDLDLTEALPLDHFRAP
                     LDLIPDSTAVILTADEEIEPALKDHWEDVTTAMHADDARHLYEDVAGPLNQRASLILV
                     RPGEGEGALRAMSAETPARNLKEAEAELRKLVEAGYRTIVAFDSRGEAERARYGFDRL
                     DTKIVDQGPVSIEPGVSLVESRLAEGFVSPRTKVAVLPFRRLLNRRRRADERAPEAVR
                     GRLTFSELRVGDFVVHEDHGVARFAGFETREVGGITRDYLYLEYKGEDRVYVPTDQFA
                     KLSRYSGGAGTPTLSALGGRKWLNMKARARGAADEIAGELVNLYAERKTRKGHAFSPD
                     SEWQMELEANFPWRETADQIEAIEAVKADMESEQPMDRLVCGDVGYGKTEVAIRAAVK
                     AASDGKQVMILAPTTILAQQHLGTFRERLADLPFRVDGVSRLRKPAEVKQVVAEWGEG
                     KVDVLIGTHRLLSRDVRAKDLGLIVVDEEQRFGVKQKELLRQMKLKVDVLSMSATPIP
                     RTLQMSMSGLRDISVIETPPEGRRPVRTYVGPWDEQLVERAIKREISRKGQVFFLHNR
                     VESLDEAAESLRALVPGVRIQVAHGQMEDQELEAAMLNFLRGDADCLVATTIIESGID
                     IPAANTLIVDRADHLGLAQAYQIRGRVGRSRERAFAYLLYPSEESLTAEASTRLATLA
                     DHTELGSGFRIAMRDLDIRGAGNLLGDEQSGHVAAVGFELYCQLIDEAVADLAGREES
                     AGEPEPVRFDIGVNAYLPASYVPFEAAKIDIHRRIAAARRPGDLRQITDELIDRFGPL
                     PEDVENLITLQQARIDLGSAGVEQVQFRGGKLTVSGIELDSEAAAAIRESVPGALYQ"
BASE COUNT          814 a         1776 c         1648 g          745 t
ORIGIN      
        1 gagccgcccc gggccaaggg ccttgcgcag gcgcgaaaga gggggcgcca gcagtgcctc
       61 tccgccactt ggcaagccct cttcggccca aagcgcctcg accagttcat cccgtctgac
      121 cggacggtgc cggttcagcg caaggtacgc aaaaagcagc ctgccctggc gccccggcag
      181 ggattcgccg agatcctcgc cgtcccagtt cacgttcaaa cggccgcaaa gtgtgatccg
      241 gggatttctc atcgcaaggt gacagtcatt tcgccgcaat ccgggcgcaa cctgtgccgt
      301 ggattctgcc agccatgtca acgccttctc tcataaacct ccttcccgcg gcggcccgca
      361 tggcgccgga aacccccgaa gaagtcgcct cggccctcgt cgaagcgggc cgccagagtc
      421 gcaaggtggt cgtcttctcg accgggcacg gcatcccgcc gatcggtgac acctcggatc
      481 gcgtcctgct cgacatgagc gcattcgaca aggtcgagat cgatccgctc gcccggaccg
      541 cccggatcgg cggcgccacc cagtggatgg agctgatctc ggccgccaac gagcacgggc
      601 tcaccgcacc gttcggatcg gccgccacgg tcggcgtcac cggttacatg atgggtggcg
      661 gcgtcggctt catgtcccgc cacctggggc tgggcagcag cgcgatccgc tcggccgaac
      721 tggtcacgcc tgacggccgg atccgccgca tcgacgccgg atccgacccg gacctgttct
      781 gggccctgcg cgggggcggt ggcggcttcg gcgtgatcac cgagctcgag atcgaactga
      841 cccccgtgcc gcggatggcc ggcggagtgc tcggctgggg catcgaggac gcggagcggg
      901 tgctcggcgc gtggtccgaa tggacccgcc ggatcgacga cgacgtgacc agcgcggtcc
      961 gcttcatgca tccgcccgaa gggccggccc tgaccgtggt ggtcgtgacc gccccccgcg
     1021 cggcggaaga gctggccgcg atcctgaagc ccctgaccga cctgaagccg atgctgaaca
     1081 cggtccggga caccgacccc gaagctttcc tgcgggagta caccgacccg gagatcgatc
     1141 cggcagagcc gccccatgcc ttcgagcacg ttctgctcga tcggcttccc ccggaggcgg
     1201 tccagctgac cgccgagttc gcccagccgg tgaccggctg cggggcgatg atggtggaga
     1261 tccgccacat ggggggagcg ctgggtcgac cggcccccgg cggcggcgcc agcgaccgga
     1321 ttgacggcga gtacctgttc ttcgtggtcg gtttccccga tgtggcggag accatgaacc
     1381 acgccgtcga tgtcctctcg gacttcggcc gcggtcggac ctacttcaac ttcatgcccc
     1441 ggaggaccgg tcgcgagacc gcctacgggc aggaagcgat cgcccggctc gccgacctct
     1501 acgaagccgt cgatccggag ggtctgatcg accatcccca cccgatttcc cgcaccgcca
     1561 gcgtcgccga cgaaaccgcc acgacccagg tcctggcaat cctccacggc tgacagtcgc
     1621 ccggcggggc aacctcaaac gcagaaatac aggcgcccgg cggtctctcc ctgaccaccg
     1681 ggcgcttttc gctttcaggt tcgtgctttg atcggtggcc gtgaccctgc gcccgacaat
     1741 cgacctgctg ctcgaggacg agcagatctc ccgcctctcg gccgaggtcc gcgaggccgt
     1801 cgccggttcc gccggaacaa cggtccgggc ggaagtctcc gccaccctgc ggccggccct
     1861 gattgccgcc atgctcgaag atgacttcgg cctggaagac cgcccggccc tgatcgtcac
     1921 gcccgacgac cggtcggccc gggagatggc cgccgcgctg ggggcattcc tcggtgaccg
     1981 catggttcgc tcctaccctt cccgcggcac cggctatgcc tcgcaggtaa ccccgccgcc
     2041 gcatctgacc ggcctcagga tcgacgccct cgattccttc ggccgcgagg aggctcaccc
     2101 cccggtcgtg gtcgcctccg cgatcgcgct cgccgaatcg gtgcccgacg cttcgcttcg
     2161 gccggccggc ttccggctca cggtcggcga ggaacatgat ccctcgcaag tcgcggccga
     2221 cctggtcgcg gccggctacg aacgggtgga ccaggtccag gaacgcggcc agttcgcggt
     2281 ccggggcggc atcctcgacg tcttctcggc gaccgaggac cgggccaccc gggtcgattt
     2341 cttcggcgac gagatcgaat cgatgcgctg gttttccacc ttcacccagc gatcgctcgg
     2401 gcaggcggaa tcaatcgagc tctccccggc ggccgaactg gacgccgaac accgcgagat
     2461 ggccgaactg atcatcgccg aggcccgtga gaacgagacc gacctcgacc tgaccgaggc
     2521 cctgccgctc gaccactttc gcgccccgct ggacctgatc cccgattcga ccgcggtgat
     2581 cctgaccgcc gacgaggaaa tcgagccggc cctgaaggac cattgggagg acgtcaccac
     2641 ggcgatgcat gccgatgacg cccggcacct ctacgaggac gtggccgggc cgctgaacca
     2701 gcgggcgtcg ctgattctcg tccgcccggg cgagggcgaa ggggcgctcc gggcgatgag
     2761 cgccgagacg ccggcccgca acctcaagga agccgaagcg gaactgcgga agctggtcga
     2821 ggccgggtac cggacgatcg tcgccttcga ctcccgcggc gaggctgaaa gggcccgtta
     2881 cggctttgac cggctcgaca cgaagatcgt cgaccagggg ccggtttcga tcgagcccgg
     2941 ggtcagtctg gtcgaatcac ggctggcgga gggcttcgtt tccccccgga ccaaggtcgc
     3001 ggtactgccc ttccgccggc tgctcaaccg gcgccgccgg gctgacgaaa gggctccgga
     3061 agccgttcgc ggccggctca ccttctccga actgagggtc ggcgacttcg tggttcatga
     3121 agaccacggg gtcgcccgct tcgccggatt cgaaacccgc gaagtgggcg gcatcacccg
     3181 cgactacctc tacctcgaat acaagggcga ggaccgggtc tatgtgccca ccgaccagtt
     3241 cgccaagctc tcgcgttatt ccggcggggc aggcaccccc accctttccg ccctcggggg
     3301 caggaagtgg ctgaacatga aggcccgcgc ccgcggcgcc gccgacgaga tcgccggcga
     3361 gctggtcaac ctctacgccg agcggaagac ccgcaagggc cacgccttca gccccgactc
     3421 ggaatggcag atggagcttg aagcgaactt cccctggcgg gagacggccg accagatcga
     3481 ggcgatcgag gcggtcaagg ccgacatgga atccgagcag ccgatggacc ggctggtctg
     3541 cggcgacgtc ggctacggca agaccgaggt ggcgatccgg gccgcggtca aggccgcctc
     3601 ggacggcaag caggtgatga tcctggcccc gaccacgatc ctcgcccagc agcatctcgg
     3661 caccttccgc gaacgcctcg ccgacctgcc cttccgggtc gacggggtca gccgcctgcg
     3721 caagcccgcc gaggtcaaac aggtggtcgc cgaatggggc gagggcaagg ttgacgtcct
     3781 gatcggcacc caccggctgc tctcccggga cgtccgggcg aaggacctcg gtctgatcgt
     3841 ggttgacgaa gaacagcgct tcggggtcaa gcagaaggaa ctgctgcgcc agatgaagct
     3901 caaggtggac gtgctttcga tgtcggcgac cccgatcccg cggaccctgc agatgtcgat
     3961 gtccgggctg agggacatct cggtgatcga gaccccgccc gagggtcgcc gcccggtccg
     4021 cacctacgtc ggcccctggg acgaacagct ggtggaacgg gcgatcaagc gggagatcag
     4081 ccgcaagggc caggtcttct tcctccacaa ccgcgtcgaa tcactcgacg aggccgccga
     4141 gagcctccgg gcgctggtgc ccggggtccg gatccaggtc gcccacggcc agatggagga
     4201 tcaggagctc gaggcggcga tgctgaactt cctcaggggt gacgccgact gcctggtcgc
     4261 caccacgatc atcgagtcgg ggattgacat ccccgcggcc aacaccctga tcgtcgaccg
     4321 ggccgaccac ctcggcctcg cccaggcgta ccagatcagg ggacgggtcg ggcggtcccg
     4381 cgagcgcgcc ttcgcctacc tgctctaccc ctcggaagag tcgctcaccg cggaagcctc
     4441 aacccgcctg gcgaccctcg ccgaccacac cgagctcggc tccggatttc gcattgcgat
     4501 gcgcgacctc gacattcgcg gggccggcaa cctgctcggg gacgagcagt ccggccatgt
     4561 cgcggcggtc ggcttcgagc tctactgcca gttgatcgac gaggcggtgg ccgacctggc
     4621 cgggcgggaa gagtcggccg gtgagccgga accggttcgc ttcgacatcg gcgtcaacgc
     4681 ctacctgccg gcgagctacg tgcctttcga agcggccaag atcgacatcc accgccggat
     4741 cgccgctgcc cgccgcccgg gagacctgcg ccagatcacc gacgagctga tcgaccggtt
     4801 cggtcccctg ccggaagacg tcgagaacct gatcacgctc cagcaggccc ggatcgacct
     4861 cggctcggcc ggggtggagc aggtccagtt ccgcggtggc aagctcacgg tctccggaat
     4921 cgagctcgat tccgaagcgg cggctgcgat ccgcgaatcg gtgccgggag cgctctacca
     4981 atg
//
LOCUS       DAOQKN010000177         4979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_9251_length_4979_cov_5.470959, whole genome shotgun sequence.
ACCESSION   DAOQKN010000177 DAOQKN010000000
VERSION     DAOQKN010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4979
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9251_length_4979_cov_5.470959"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PLJ64_10440"
     CDS             complement(<1..202)
                     /locus_tag="PLJ64_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNC93944.1"
                     /translation="MSFLVAFLAFSALIVLHEAGHFTAAKAVGMRVERFFLFFPPKLV
                     SIKRGETEYGIGAIPLGGFVRIT"
     gene            complement(232..1380)
                     /gene="dxr"
                     /locus_tag="PLJ64_10445"
     CDS             complement(232..1380)
                     /gene="dxr"
                     /locus_tag="PLJ64_10445"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNC93945.1"
                     /translation="MSADKRVLILGSTGSIGTQALEIIAGREDLEVAGLAVGRDWQTA
                     VAQAKGFSVGTIAVADETAAAEATVGFDGNVLAGEPGVIELIGATAPDIVLNGIVGAA
                     GLGPTIAALTQGIDVALANKESLVIGGELVTELASAAGAKLLPVDSEHSALFQLVENE
                     DRAAIDRIVLTASGGPFRGCTDLAGITVEQALDHPTWAMGGRITIDSATLMNKGFEMI
                     EAHHLFDLPYEQIAVVVHPQSIVHSLIDLRDGATLAHLGYPDMRVPIAYALTWPERAE
                     VPVERLDLAKVGSLDFEAPDPDTFRCLALAREAGESGGIAPCVLNAADEVAVAAFLEG
                     KVSFIDIARVIEQTLDDIGSGPAKGFEELFAVDEASRERASEIISGLN"
     gene            complement(1428..2222)
                     /locus_tag="PLJ64_10450"
     CDS             complement(1428..2222)
                     /locus_tag="PLJ64_10450"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNC93946.1"
                     /translation="MGETAKRILVAVPWIIVVIAIIVLGGPWFAAALAVFGCLGLREF
                     FQMAEEYRPFRIPAYLSLVGIVASAWLGTQFNVLMFLALPFLLIFFGAARRKDRTGVT
                     GSVAATLLGIYWIGLALAHAVLLRELPDHGGALVVDVLVGTFACDTGAYAVGRMFGSH
                     KFAPNLSPNKTIEGLIGGFLIGMLGVWCAGLYQDWLSGVDALILGAAVAAVAPVGDLF
                     ESMIKRDLGTKDTGNLFGPHGGLLDRLDAVFFTIVVGYYVSVALVY"
     gene            complement(2222..2905)
                     /gene="uppS"
                     /locus_tag="PLJ64_10455"
     CDS             complement(2222..2905)
                     /gene="uppS"
                     /locus_tag="PLJ64_10455"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HNC93947.1"
                     /translation="MNAPTGPKYVAIITDGNGRWAQSRGLPVKEGHKAGADTVKARLR
                     DAVEFGVKELTVFSFSTENWSRSPEEVSALMEMFSRRIVDETPELNEEGVRMRFIGRR
                     GGVSEKLQQQMDEAESMTAANDTITFFIAFNYGGRAEIIDAAKKFDGSTEEEFQQCLY
                     APEMHDPELLIRTSGEQRISNYLLWQCAYSEFVFRDELWPDFTREAFAASLEEYGNRQ
                     RRFGTREPV"
     gene            complement(2902..3459)
                     /gene="frr"
                     /locus_tag="PLJ64_10460"
     CDS             complement(2902..3459)
                     /gene="frr"
                     /locus_tag="PLJ64_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNC93948.1"
                     /translation="MIDDLLADARERMAKTVESTRGEFATVRTGRATPHLLDRITVDY
                     YGANTPLNQMANVAASEARLLTVTPYDKGALGLIEKAIQESDIGLTPSNDGNVIRLQI
                     PDLTEERRKEMVKVVHGVAEDGRVAVRNVRRDVMAELRELKKEGEVGEDDERRGEEAL
                     QKQTDASIAEIDSMLKGKEEEILEV"
     gene            complement(3477..4217)
                     /gene="pyrH"
                     /locus_tag="PLJ64_10465"
     CDS             complement(3477..4217)
                     /gene="pyrH"
                     /locus_tag="PLJ64_10465"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005328724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNC93949.1"
                     /translation="MSDGEPRFRRIMLKLSGEALMGKLEFGTDAQEVERIARQIGIIR
                     DRGVEVAIVVGAGNIYRGVDGAAQGMDRATGDYMGMLATLLNALTLQDVLEKHGHFTR
                     VVSAIDVKEVAEPYIRRRAMRHLEKGRIVIFAAGTGNPFFTTDTAAALRALEIHAETI
                     LMAKNGVEGVFDSDPATNPGATFIPEITHREAIERGLKVMDSTALSLCMDNDLPIYVF
                     NMAEEANMDRIVTGEKVGTLVSSRVAAN"
     gene            complement(4240..4845)
                     /gene="tsf"
                     /locus_tag="PLJ64_10470"
     CDS             complement(4240..4845)
                     /gene="tsf"
                     /locus_tag="PLJ64_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNC93950.1"
                     /translation="MADIKAADVKALREMTGAGMMDAKAALIEAGGDTDRAVELLRVK
                     GQASAAKRSDRGTSEGVVASYIHANKKVGALVEVQCETDFVARNEDFAEFAYEVAMHV
                     AAASPTHVSKEDIPEDQVAAERRVFEEKAKEEGKPENVVEKIVEGQVNKWARDIALLE
                     QEHVNTDKHDSKTIEQLREELAAKVGENVVISRFSCFRIGE"
BASE COUNT          830 a         1617 c         1588 g          944 t
ORIGIN      
        1 cggtgatccg cacgaaacca ccaagcggga tcgccccgat cccgtattcg gtctcccccc
       61 gcttgatcga aaccagcttc ggcgggaaga agaggaagaa acgctcaacc cgcattccca
      121 ccgccttggc ggcggtgaag tgcccggcct cgtggaggac gatcagggca ctgaaagcga
      181 ggaatgcgac aagaaagctc atgagaagtc agggccggcc ggcgggccga ttcagttcag
      241 accggaaatg atctcagaag cgcgttcccg gcttgcttcg tccaccgcga aaagctcctc
      301 aaagcctttg gcggggccac tcccgatgtc atcgagggtc tgctcgatca ccctggcgat
      361 atcgatgaag gagaccttgc cttcgaggaa cgcggccacc gcgacctcat cggcagcgtt
      421 caggacgcaa ggggcgattc cgccggattc ccctgcttcc cgggcaaggg ccaggcagcg
      481 gaaggtgtcc ggatcgggtg cttcgaaatc gaggctgccc accttcgcga ggtcgaggcg
      541 ctcgaccgga acctcagccc gctccggcca ggtcagggcg taggcgatcg gcacccgcat
      601 gtccgggtaa ccgaggtggg ccagggtggc gccgtcacgc agatcgatca gcgaatggac
      661 gatcgactgg ggatggacca ccacggcgat ctgctcgtag ggaaggtcga acaggtgatg
      721 ggcctcgatc atctcgaagc ccttgttcat cagggttgcc gagtcgatcg tgatccggcc
      781 gcccatcgcc catgtcggat ggtctagagc ctgctcgacg gtgattcctg ctaggtcggt
      841 gcagccacgg aagggaccgc cggaagcggt aaggacgatg cggtcgatcg cggcccggtc
      901 ctcgttttcg accagctgga aaagggcgga atgctcggaa tcgaccggca ggagcttcgc
      961 accggcggcc gaggcaagct cggtgaccag ctccccgccg atgaccaggc tttccttgtt
     1021 ggcgagggcg acatcgatgc cctgtgtcag ggctgcgatg gtcgggccga gtccggccgc
     1081 cccgacgatc ccgttcagca cgatgtcggg agcggtggcg ccgatcagct cgatcactcc
     1141 cggctccccg gccagcacgt tcccgtcgaa gccgaccgtg gcttcggcgg cagcggtctc
     1201 gtcggcgacc gcgatggtcc cgaccgagaa gcccttggcc tgggcgaccg ccgtctgcca
     1261 gtcccggccg accgccaggc ctgccacttc caggtcttcc cggccggcga tgatctcgag
     1321 ggcctgggtg ccgatcgagc cggtcgaacc gaggatcagg acccgcttgt cagcactcat
     1381 tttcctgatt ccggaagtcc gggaattgag gaatcagcgc tcatggccta gtacaccagc
     1441 gctacggaga cgtagtagcc gaccacgatc gtgaagaaga ccgcgtcgag gcggtcgagc
     1501 agaccgccgt gcgggccgaa caggttgccg gtgtccttgg tccccagatc ccgcttgatc
     1561 atcgactcga agaggtcacc caccggggcg acggcggcca ctgccgcacc cagaatcagt
     1621 gcgtccactc cggaaagcca gtcctggtag agaccggcgc accagacccc gagcatgccg
     1681 atcaggaatc cgccgatcag cccttcgatc gtcttgttcg gtgaaaggtt cggcgcgaac
     1741 ttgtggctgc cgaacatccg gccgaccgcg taggccccgg tgtcacaggc gaaggtgccg
     1801 accaaaacgt cgaccacgag ggcgccaccg tgatcaggaa gttcgcgcag cagtaccgca
     1861 tgagcgaggg ccagaccgat ccagtagatc ccgagcaagg tagccgcgac cgagccggtc
     1921 acccccgtcc ggtccttgcg gcgggccgcc ccgaagaaga tcagcaggaa cggcagggcc
     1981 aggaacatca gcacgttgaa ctgggtgccc agccaggcgg aagcgacgat tccgaccagc
     2041 gacagatagg ccgggatccg gaacggccgg tattcctcgg ccatctggaa gaactcgcgc
     2101 agtcccaggc agccgaacac ggccagggcg gcagcgaacc agggcccgcc gagaacgatg
     2161 atcgcgatca cgacgatgat ccagggcacg gcgaccagga tgcgcttggc tgtctcgccc
     2221 atcagaccgg ctccctggtc ccgaaacggc gctgccggtt gccgtattcc tccagcgaag
     2281 cggcaaaggc ctcgcgagtg aaatccggcc agagctcgtc ccggaagacg aattccgagt
     2341 aggcgcattg ccagagcagg tagttggaga tccgctgttc gccgctggtc cggatcagca
     2401 gctcgggatc atgcatctcc ggcgcgtaca aacactgctg gaactcttcc tcggtggagc
     2461 cgtcgaactt cttcgcggcg tcgatgatct cggcccggcc gccgtaattg aaggcgatga
     2521 agaaggtgat cgtgtcattg gcggcggtca tcgattcggc ttcgtccatc tgctgctgga
     2581 gcttctccga gacgcctccc cggcggccga tgaagcgcat gcgcacgcct tcctcgttca
     2641 gctccggagt ttcgtcgacg atccgccggg agaacatctc catcagggcc gacacctcct
     2701 ccggtgaccg ggaccagttc tcggttgaaa acgagaagac cgtcagttcc ttcacgccga
     2761 attcgaccgc gtcgcgaagc cttgccttga cggtgtcggc gccggccttg tggccttcct
     2821 tgaccggcag tccgcggctc tgggcccagc gcccgttgcc gtcggtgatg atggctacgt
     2881 acttaggtcc ggtgggtgcg ttcacacctc gaggatctct tcttccttgc ccttgagcat
     2941 ggaatcgatc tccgcgatgg aggcgtcggt ctgcttctgc agggcttctt cgccacggcg
     3001 ctcgtcgtct tctccgactt cgccttcctt cttcagctct cgcagttcgg ccatcacgtc
     3061 gcgacgcacg ttgcggacgg cgacccgccc gtcctcggcg acaccgtgaa cgaccttgac
     3121 catctccttg cgccgttcct cggtcaggtc cgggatctgg agccggatca cgtttccgtc
     3181 attcgagggc gtgaggccga tgtccgattc ctggatcgcc ttctcgatca ggccgagggc
     3241 acccttgtca tatggggtga ccgtgagcag ccgggcctcg gaggccgcga cgttcgccat
     3301 ctggttgagc ggcgtgttgg cgccgtagta gtcgaccgtg atccgatcga gcaggtgcgg
     3361 agtggcgcga ccggtacgga ccgtagcgaa ctcgccacgg gtcgattcga ccgtcttggc
     3421 catcctttcc ctggcgtctg caagcaggtc gtcgatcatg gattctcctt tggcgatcag
     3481 ttagcggcga ccctggagct gaccagcgtg cccacttttt cgccggtcac gatcctatcc
     3541 atattggcct cctcagccat gttgaagacg tagatcggca ggtcgttgtc catgcagagg
     3601 ctgagtgcgg ttgagtccat caccttgagg ccgcgctcga tggcctcgcg gtgcgtgatc
     3661 tcggggatga aggtcgcgcc cggattggtc gccgggtcgc tgtcgaagac cccttccacc
     3721 ccgtttttgg ccatcaggat ggtttcggcg tggatctcca gcgcgcgcag ggccgcggcg
     3781 gtgtcggtcg tgaagaacgg attgccggtg ccggccgcga agatcacgat ccggcccttc
     3841 tcgaggtggc gcatcgcccg acggcgaatg tagggctcgg cgacttcctt gacgtcgatc
     3901 gccgaaacca cccgggtgaa gtggccgtgc ttttctagca cgtcctgaag ggtcagggca
     3961 ttgagcaggg ttgccagcat gcccatgtag tcaccggttg cccggtccat gccctgggcg
     4021 gcaccgtcaa ccccgcgata gatgttgccc gccccgacca cgatggcgac ttcgacgccg
     4081 cggtcccgga tgatgccgat ctgccgggcg atccgctcga cttcctgcgc atcggtcccg
     4141 aattccagct tgcccatcag ggcttcgcca gacagtttca gcattatccg cctgaaccgc
     4201 ggctctccgt cgctcatcag ctggtcggaa atctacctct tattcgccga tccggaagca
     4261 ggagaaacgg ctgatcacga cgttctcgcc gaccttggcg gccagttctt cgcgcagctg
     4321 ctcgatcgtc ttcgaatcgt gcttgtcggt gttgacgtgt tcctgctcga gcagggcgat
     4381 gtccctggcc cacttgttga cctggccttc gacgatcttc tcgaccacgt tctcgggctt
     4441 gccctcttcc ttggccttct cctcgaatac ccggcgctcg gccgcgacct ggtcctcggg
     4501 gatgtcttcc ttcgagacat gggtcggaga ggcggcggcg acatgcatcg ctacctcgta
     4561 ggcaaactcg gcgaagtcct cgttgcgggc gacgaagtcg gtttcgcact ggacttcaac
     4621 cagggcgccg acttttttgt tcgcgtggat gtaagaggca acgacgcctt cagaggtgcc
     4681 acggtcggag cgcttggcgg ccgaagcctg ccccttgacc cgcagcagtt caaccgcgcg
     4741 gtcggtgtct ccaccggctt cgatgagcgc ggctttcgcg tccatcattc cggcgccggt
     4801 catctcgcgc agcgccttga cgtctgcggc cttgatgtct gccatcaggc gttctcctcg
     4861 ttcttctcgt cggccggagc ttccccggcc ggggtttctt cagtttcggt tgccgcttcg
     4921 gcggccggct cggggtccgg ggccacggcc tgcgggtgcg ggttgtccgg cacggcctg
//
LOCUS       DAOQKN010000178         4914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_9452_length_4914_cov_4.306647, whole genome shotgun sequence.
ACCESSION   DAOQKN010000178 DAOQKN010000000
VERSION     DAOQKN010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4914
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9452_length_4914_cov_4.306647"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="PLJ64_10475"
     CDS             <1..605
                     /locus_tag="PLJ64_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93951.1"
                     /translation="FTLLCGFVFCLIGFIFMLTLWDEGAAYWQVLAGYLPVGIGVGLA
                     GTPSSNSLTSSVPVKRAGMASGTADLQRDLGGALMQSILGSVLTSAYASSFASQIAGN
                     PEASQKVSESTQTQLERSFGSAVTVAEQHPQYAPQIVHAAQTAFLNGSDSAYVVGIAA
                     IVLGAVLVFFAFPKKEQEHALLAGFHAEDTGEPVPATATA"
     gene            619..1809
                     /locus_tag="PLJ64_10480"
     CDS             619..1809
                     /locus_tag="PLJ64_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HNC93952.1"
                     /translation="MNRTFVIGVGMTRFDKPGTKEGDYPDWAVEAGRKALEDAGITYD
                     EVQCAYVGYCYGESTSGERAVYGLGLSGIPIINVNNNCSTGSSALYLARQSVKGGLAD
                     CALALGFEKMEKGSLGAKYTDRTQPLDRHFIELTQLRQWEDAPPAPQIFGAAGREHME
                     KYGSRPEHFAWIGYKNHKHSVNNPYAQFQDEYTLQQIQDAPEIYSPLTKLQCSPTSDG
                     SGAAIVASEKFVDEHDLWEKAIEITGQALVTDTRETFDDDSAISLVGYEMSRLAAEQA
                     LNEAGTGIDEVDVVELHDCFSANELITYEALGLAERGHGHELVEAEATTYGGSTVVNP
                     SGGLISKGHPLGATGLAQCSELTWQLRGDADARQVEGAGKALQHNIGLGGAAVVTVYE
                     KPAA"
     gene            complement(1883..2701)
                     /locus_tag="PLJ64_10485"
     CDS             complement(1883..2701)
                     /locus_tag="PLJ64_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93953.1"
                     /translation="MKRGSGETMRDARPAAGQGGRKPSPALVVAIIALIVALGGTSYA
                     AFKLPKNSVGTKQLKKNAVTVKKLRKNSVNSAKVRNRSLLAKDFRKGQLPRGATGPAG
                     PIAGTPAGGSLSGTYPDPAIASGAIGAAQLQAAPGARAITNVPQSTATATNIIFTANA
                     TSYNKGGLYSNAEDAMVVTVPGTYVVSGAVTWEGNANGSRQTRILLNGDVRNVATASP
                     LNAGALRQTVSLIDHFNAGDRIQLGGFQTSGGSLDTLVSTGAGAVQLSATWVSP"
     gene            complement(2774..3400)
                     /locus_tag="PLJ64_10490"
     CDS             complement(2774..3400)
                     /locus_tag="PLJ64_10490"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HNC93954.1"
                     /translation="MEVDLNFYLQSIVAVVVITDPLTRGIFFRQLTANEPERRGEFVR
                     RITIVVAVTLFGAALVGRELLDVMGINLGAFGVAGGLVVALMGFEMLFGGEPSRAQGG
                     EQSREQPESFDGSVVVPYAIPFIAGPGAITTVITIAASAENGEGTIVALVAVAVAVIL
                     LPIGHLLVAKHVNLSDQAESVVTKIGGLLITTIGIQLMLSGIQRFFEG"
     gene            3493..4827
                     /locus_tag="PLJ64_10495"
     CDS             3493..4827
                     /locus_tag="PLJ64_10495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93955.1"
                     /translation="MERQVPNSGQWRMCLPVIALLAALLALAAAPALALADGKPDLSF
                     GKKGTSAFDPDPLSLQSPSDMVTDSKGRIVMGGTTFNGPGGNMAWFTRLNRNGSVDST
                     FGTGGFTTFPVPAQTYIATVAVDSEGRIVAAGNYDLPMNRDIWVLRLKSDGHLDSGFG
                     IGGGVGFDFGSFDLGYAAVVGKDGLIYVAASTGDGDSDKATVLRYTSDGDPDSTWGTG
                     GAVRLEVGGTSSRAEAIRLDRRGRVVLGGERVNPDDSSDFLAIRLTRKGALDKTFNGR
                     GFRTIRFMKGQRAEVSDMRVDGKGRILLLGAGDIAPDGLPDGLLARLTPNGKPDKSFG
                     LSGKVVKSIFGTRMYLAHVRIDRAGRIVTAGYGRDPRNAEQALLIRFESNGRSDRSWG
                     RNGVRKLTFGQPEGEGTAMTIDGKGRYLVGGYVFDTGLSYVTRLLVKYPRHR"
BASE COUNT          863 a         1614 c         1598 g          839 t
ORIGIN      
        1 gttttaccct gctctgcggc ttcgtcttct gcctgatcgg tttcatcttc atgctcacgc
       61 tctgggatga gggtgcggct tactggcagg tcctggccgg ctaccttccg gtggggatcg
      121 gggtcggcct ggccggcact ccctcctcca actcgctgac cagctcggtt ccggtcaaac
      181 gcgccgggat ggcctccggg accgcggacc tgcagcggga cctgggcggc gcattgatgc
      241 agtcgatcct cgggtcggtc ctgacttccg cctacgcgag ctcgttcgcg tcccagatcg
      301 ccggcaatcc ggaagcgagc cagaaggtca gcgaatcgac ccagacccag ctcgaacggt
      361 ccttcggcag cgcggtcacg gttgccgagc aacatcccca atacgcgccc cagatcgtgc
      421 acgcggcaca gaccgcgttc ctgaacggca gcgactccgc ctatgtggtg gggatcgccg
      481 cgatcgtcct tggtgcggtc ctcgtcttct tcgccttccc gaagaaggag caggaacacg
      541 cgctgctggc aggattccat gccgaggaca cgggcgagcc cgttcccgcc accgcaaccg
      601 cctgagggag ttggagagat gaaccgtact tttgtgatcg gggtcggaat gaccaggttc
      661 gacaagcccg gaaccaagga aggcgattat ccggactggg ccgtggaagc cggccggaag
      721 gcacttgagg acgcgggcat cacctacgac gaagtccagt gcgcctatgt cggctactgc
      781 tacggggaat cgacctccgg cgaacgggcg gtctacgggc tcggcctttc cgggattccg
      841 atcatcaacg tgaacaacaa ctgctcgacc ggttcctcgg ccctttacct ggcccgccag
      901 tcggtcaagg gcggcctcgc cgattgcgcc ctggcgctcg gcttcgagaa gatggagaag
      961 ggctcgcttg gtgccaagta caccgaccgc acccagccgc ttgacaggca tttcatcgag
     1021 ttgacccaac tccgccagtg ggaagacgca ccgcccgccc cgcagatctt cggcgcggcc
     1081 ggtcgcgaac acatggagaa gtacgggtcc aggccggagc atttcgcctg gatcggttac
     1141 aagaaccaca agcactcggt caacaacccc tacgcccagt tccaggacga gtacacgctc
     1201 cagcagatcc aggacgcccc cgagatctac tcgccactga ccaagctgca gtgctccccc
     1261 acctcggacg gctcgggtgc cgcgatcgtc gcctccgaga agttcgtcga cgagcatgac
     1321 ctgtgggaga aggcgatcga gatcaccggc caggccctgg tcaccgacac ccgggagacc
     1381 tttgacgacg attcggcgat cagcctggtc gggtacgaga tgagccggct tgccgccgag
     1441 caggccctga acgaggccgg caccgggatc gacgaggtcg acgtggtcga actccacgac
     1501 tgcttcagcg ccaacgagct gatcacctac gaggcactcg gcctggccga gcgcggccac
     1561 ggccacgaac tggtcgaggc cgaagccacc acctacgggg gcagcacggt cgtcaacccg
     1621 tccggcggcc tgatctcgaa gggccatccg ctcggtgcga ccggcctcgc ccagtgttcg
     1681 gagctcacct ggcagcttcg cggcgacgcc gacgcgcgcc aagtcgaggg cgccggcaag
     1741 gccctccagc acaacatcgg cctcggcggc gccgccgtcg tcaccgttta cgagaagccc
     1801 gccgcctgag ccgaaagctg cgcatcggga tggcagaagt cagtcgatat gactgacctt
     1861 tgccatctcg atcaggttcc ggtcaggggc tgacccaggt ggcgctgagc tggacggcac
     1921 ccgccccggt ggagaccagg gtgtccaggc tgccgcccga tgtctggaag ccgccgagct
     1981 ggatcctgtc gccggcgttg aagtggtcga tcagggagac cgtctgccgc agcgccccag
     2041 cgttgagcgg gctggccgta gccacgttgc gaacgtcacc gttgaggagg atgcgcgtct
     2101 ggcgcgagcc gttggcgttg ccctcccagg tcaccgcgcc gctcaccacg taggtcccgg
     2161 gcacggtgac gaccatggcg tcttccgcgt tcgagtagag accgcccttg ttgtatgaag
     2221 tcgcattcgc ggtgaaaatg atgttggtcg cggtcgccgt ggactgcggg acgttggtaa
     2281 tggccctggc ccctggtgct gcctgaagct gggcggcgcc gatcgctccg ctggcgatcg
     2341 ccgggtccgg ataggtgccg ctgagcgagc cgccggccgg ggtgccggcg atcggaccgg
     2401 ccggaccggt cgcgccccgg ggaagctggc ccttccggaa gtccttggcg agcagcgaac
     2461 ggttcctgac cttggccgag ttgaccgagt tcttcctcag tttcttgacc gtcaccgcgt
     2521 tcttcttcag ctgtttcgtt ccgaccgaat tcttcggcag cttgaacgcc gcataactgg
     2581 ttccgcccag cgcaacgatc agggcgatga tcgccaccac cagcgccggt gacggtttcc
     2641 tgccgccctg cccggctgcc ggtcttgcgt cccgcatggt ctcccccgat cctcgcttca
     2701 acagagcttc cagcctaccc tccgcgccag gccacggcaa gcgccggcgc aaacaacccg
     2761 gactccccga aactcaaccc tcgaaaaagc gctggatgcc gctgagcatc aactggatcc
     2821 cgatcgtggt gatcagcagg ccgccgatct tggtcacgac tgactccgcc tggtcggaga
     2881 ggttcacgtg cttcgcgaca agcaggtggc cgatcggcag gaggatcacc gccaccgcga
     2941 ccgcgaccag ggcgacgatc gtaccctcgc cgttttcggc cgaagctgcg atcgtgatca
     3001 cggtggtgat cgcgcccggt ccggcgatga acggaatcgc gtaggggacg acgacgctgc
     3061 cgtcgaagct ctcgggctgc tcgcgggatt gttcgcctcc ctgagcccgg ctcggttcac
     3121 cgccgaagag catctcgaaa cccatcaggg ccacgaccag gccaccggcg acaccgaagg
     3181 ccccgagatt gatccccatt acgtcaagca gctcacggcc gaccagcgcc gctccgaaaa
     3241 gtgtcacggc gaccacgatc gtgattcgcc ggacgaactc gccccggcgc tcgggctcgt
     3301 tcgcggtcag ctggcgaaag aagatccccc gcgtcagggg atcggtgatc accaccaccg
     3361 cgacgatcga ctgcaggtag aagttgagat cgacctccat ccctgcccgc agggtaaaga
     3421 gcgacccggt cgcccgggtt gccatccgcg tgggtcgggg ataggttggg gacagttcaa
     3481 gtgaggtgga gaatggaacg gcaggttccc aattcaggtc agtggcgcat gtgccttccg
     3541 gtcattgcgt tgctggctgc gcttctggct cttgcggcag cgcccgccct cgcgctcgcg
     3601 gatgggaagc ccgacctgtc cttcggcaag aaggggacct ctgcgttcga tcccgacccg
     3661 ctttcgctcc agagtccttc ggacatggtc accgactcga agggtcggat cgttatgggc
     3721 gggaccacct tcaacggacc gggcgggaac atggcctggt tcacccggct gaaccggaac
     3781 ggcagcgtcg actcgacctt cggaaccggc ggtttcacaa cgtttccggt tccagcgcag
     3841 acgtacatcg caacggtcgc ggtcgattcc gagggcagga tcgtggccgc gggaaactat
     3901 gaccttccga tgaatcgtga catctgggtg ctgcgtctga aatcggacgg gcacctcgac
     3961 tcaggcttcg gcatcggcgg cggagtcggt ttcgactttg gcagtttcga cctcggttac
     4021 gccgcggtgg tcggcaagga cggcttgatc tacgtggccg cttcgaccgg tgacggcgac
     4081 tcggacaagg ccacggtgct ccgctacaca tcggatgggg atcccgattc cacctgggga
     4141 accggtggtg ccgtccggct ggaagtcggt ggtacctcct cccgggcgga agcgatcagg
     4201 ctcgaccggc gtggcagggt cgtcctgggc ggcgagcgcg tcaatcccga tgacagctcg
     4261 gacttcctgg cgatccggct cacccgcaaa ggggccctgg acaagacctt caacgggaga
     4321 gggttcagga ccatcaggtt catgaagggc cagcgggccg aagtcagcga catgagggtc
     4381 gacggaaagg gccggatcct cctgctcggg gcgggcgaca tcgctcccga cggacttccg
     4441 gacgggttgt tggccaggct cacgccgaat ggcaagcccg acaagagctt cggactcagc
     4501 ggcaaggtcg tgaagagcat cttcggcacc cgcatgtatc tggctcacgt caggatcgac
     4561 cgggcgggcc ggatcgtcac ggcgggttac gggagggacc cgagaaatgc cgagcaggcg
     4621 ttgctcattc gcttcgaatc gaatgggcgc tccgaccgaa gctggggcag gaacggggtg
     4681 agaaagctca cctttggcca gcccgaaggt gaagggactg cgatgacgat cgacggcaag
     4741 ggccggtacc tggtcggcgg gtacgttttc gacacgggcc tgtcatatgt aacgcgactt
     4801 ctcgtcaagt acccgcgcca ccggtaggac gcaccggccg cccggtaggg tgccctccgt
     4861 ggctgactac atcttcacca tgtaccaggt ctcccgggtc catccgcccg acaa
//
LOCUS       DAOQKN010000179         4883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_9558_length_4883_cov_5.206297, whole genome shotgun sequence.
ACCESSION   DAOQKN010000179 DAOQKN010000000
VERSION     DAOQKN010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4883
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9558_length_4883_cov_5.206297"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="PLJ64_10500"
     CDS             complement(<1..480)
                     /locus_tag="PLJ64_10500"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC93956.1"
                     /translation="MSSAAISIKGLRKTYDDLEAVRGVDLEVFPEEIVAVLGPNGAGK
                     TTTVEILEGYRDRDGGEVSVLGVDPAKPTPAWRDRIGIVLQSTTLEPALTVRESLQQY
                     AGYYRNPREVDEVIGLVGLEEQAGTRARKLSGGQQRRLDVGIALVGDPDLLFLDEPTT
                     "
     gene            complement(508..1422)
                     /gene="rapZ"
                     /locus_tag="PLJ64_10505"
     CDS             complement(508..1422)
                     /gene="rapZ"
                     /locus_tag="PLJ64_10505"
                     /inference="COORDINATES: protein motif:HMM:NF015620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HNC93957.1"
                     /translation="MAAAEPGEDRSEDEGQREDPVEMVVITGLSGAGKSEAIAAFEDA
                     GYFCVDNLPPQMIGALGELFRLPGSGVTKAAVVSDARGGEYFEQLNQVLDDLEGDGIK
                     PKLIFLEAHEEILTDRYKETRRRHPSAPEGRITEGIEREREILAPLRARADVVIDTSG
                     ESASTLRRRISEDLIDTGSSSLMVVNLLTFGFKNGPPRDADITLDVRFLPNPHYVDDL
                     RPLTGLDQEVRDYVEAGTMAGEFYGRLLPLLEFLIPAYVAEGKSHLTIAIGCTGGRHR
                     SITVAERIWREFSTRDDVKVRVTHRDIA"
     gene            complement(1428..3326)
                     /gene="uvrC"
                     /locus_tag="PLJ64_10510"
     CDS             complement(1428..3326)
                     /gene="uvrC"
                     /locus_tag="PLJ64_10510"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNC93958.1"
                     /translation="MAESTGKQQSKEPDREARIVAERRSLPDVPGVYLFRDRDDEVIY
                     VGKAVSIRKRVASHFSGGNAGMTRLIERIEFLATESESEALIAEQSFIKRYRPRFNIK
                     LRDDKSYPYVAVSLDEDYPRVYFTRERHRPGRAYFGPFSSARRVRDTLDLLGKLFQYR
                     TCEGKEPGRRSGSPCLDYFIKRCGAPCVDYISQADYRANIDRVIDFLSGRYDQIERDL
                     DTRMKEAAAAQDFEAAAVFRDRLASVQSLFERQRISGSTVGTADLIGVATEGTDANAQ
                     VFQVRDGILAERQSFYLENPAERGIDEVTAEFIEQYYGASPAVPKRVVLGPYMSEKLA
                     EVETFLAGRRANRATEVRIAERGDTRRLREMAERNAKLALDQDRLQSEHRQQRRIEAL
                     SQLGDALGMDELPVRIEGFDISNLGGQHTVASMVVFEGGRPKKSDYRRFRVRGEEGRP
                     GPDDFASMEEVLGRRIAQYRQQADLSPHDRDRDESFATLPSVIMIDGGKGQLSAGVRA
                     LREFIDLGVTVVSLAKREEEVFTPGSSLPLDLKADSEANLLLQRVRDEAHRFAITHHR
                     GRRDRAMTASVLDGIRGVGPNRKRLLIRHFGSPDRLLEASREEIEAVPGLPGKVAREI
                     HDQLNRIG"
     gene            3374..4198
                     /locus_tag="PLJ64_10515"
     CDS             3374..4198
                     /locus_tag="PLJ64_10515"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNC93959.1"
                     /translation="MAAETLTPPELKTIDIAIDGEIGTMTLNRPETFNAMSPDLIEDL
                     ATAFAWLADQAPLRGLIITGAGKAFCAGGDLNWFKKGQEDPDTDIVASVRRGAELLHS
                     GIVDLQRIPYPVIAALNGPTAGAGFSLALACDIRICSEDAFLACAYGRIGVSPDGGMT
                     YFLPRVVGPSKAIEILLNDPNLSPADALEAGLVSEVVAPDQLLDRAREKADKLAKKSP
                     YYVKMAKHLVHQSLDNSITEQLQLERHGIANGMGTEDSKNAIEAFLTGGTAEFKGE"
     gene            complement(4236..4721)
                     /locus_tag="PLJ64_10520"
     CDS             complement(4236..4721)
                     /locus_tag="PLJ64_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HNC93960.1"
                     /translation="METLAIEGPEQVQELVGKTVGPTGWREVTQQMIDTFADLSGDHQ
                     WIHVEVERAKNESPFGTTIAHGNLTLSLVDGFRLDLIEVKSGFALGVNYGWNKVRFPA
                     PVPAGSKVRASAELTEANDLGNGWLELITRFTLEVQAPDGSIGEKPCFVGESVTRALK
                     A"
BASE COUNT          787 a         1641 c         1589 g          866 t
ORIGIN      
        1 ggtggtcggc tcgtccagaa agagcaggtc ggggtcgccg accagggcga tgccgacgtc
       61 gagccggcgc tgctggccgc cggacagttt gcgggcacgc gttccggcct gttcctcgag
      121 accgaccagt ccgatcacct cgtcgacctc gcggggattg cggtagtagc cggcatattg
      181 ctgaagcgat tccctgaccg tcagggccgg ctccagcgtg gtgctctgca gcacgatccc
      241 gatccggtcc cgccaggcgg gggttggttt ggcgggatcc acgccgagga cactgacttc
      301 gcccccgtcg cggtcacggt agccctcgag gatctcgacc gtggtcgtct tcccggcccc
      361 gttcgggcca agcacggcca cgatctcctc ggggaagacc tcgagatcga ctccccggac
      421 cgcctcaaga tcgtcgtagg tcttgcgcag gcccttgatg cttattgccg cactggacac
      481 ggccaacaac cctacggggt tggggaatca ggcaatgtcc cggtgcgtga cccggacctt
      541 cacgtcgtcg cgggtcgaga actcgcgcca gatccgttcc gccacggtga tcgaccggtg
      601 ccggcccccc gtgcagccga tcgcgatcgt gaggtggctc ttgccctccg cgacataggc
      661 cgggatcagg aactcgagca ggggcagcag ccggccgtag aactcgccgg ccatggtgcc
      721 ggcttcaacg tagtcacgga cttcctggtc gagaccggtc agcggcctca gatcgtccac
      781 gtagtggggg ttcggcagaa agcggacgtc aagggtgatg tcggcatccc gtggcggccc
      841 gttcttgaag ccgaaagtga gcaggttgac caccatcagc gaactggacc ccgtgtcaat
      901 cagatcctcc gagatccggc gacgcagggt cgaggccgat tcgccggaag tgtcgatcac
      961 cacatcggcc cgggcccgga gcggggccag gatctccctt tcgcgttcga tcccttcggt
     1021 gatccggcct tccggggccg agggatggcg gcggcgggtt tccttgtacc ggtcggtgag
     1081 gatctcctcg tgggcctcga ggaagatcag cttcggcttg atcccgtcgc cttccaggtc
     1141 gtcgaggacc tggttcagct gctcgaagta ctcgccgccg cgggcatccg agaccacggc
     1201 ggccttcgtc actccgctgc ccggcaggcg gaagagctcg ccgagcgcgc caatcatctg
     1261 gggcggcagg ttgtccacgc agaagtagcc cgcgtcctcg aaggcggcga tcgcctccga
     1321 cttgcctgcc ccggagagac cggtgatcac caccatctcg accggatcct ctctttgacc
     1381 ctcgtcttcg cttcggtcct cgcccggctc tgcggccgcc atgggcacta tccgatcctg
     1441 ttcagttggt cgtgaatctc gcgggccacc ttaccgggca gcccgggcac cgcttcgatc
     1501 tcttcgcggc tggcctcgag caggcggtcg ggcgagccga aatgcctgat gagcaatcgc
     1561 ttgcggttcg gtccgacccc gcggatcccg tccaggaccg aggccgtcat ggcacggtcg
     1621 cgacggcccc ggtgatgggt gatcgcgaag cggtgggctt cgtcccggac ccgttgaagc
     1681 agcaggttcg cttccgagtc ggccttgagg tcgagcggca gggatgaacc cggggtgaag
     1741 acctcctcct cgcgcttggc gaggctgacc acggtgacgc cgagatcgat gaactcccgg
     1801 agggcccgga ccccggccga gagctgaccc ttgccgccgt cgatcatgat cacgctcggc
     1861 agcgtggcga agctttcgtc gcggtcccgg tcatgcggag aaaggtccgc ctgctgccgg
     1921 tactgcgcga ttcgccggcc cagcacctcc tccatcgagg cgaagtcgtc cgggcccgga
     1981 cggccctcct ccccccgcac ccggaaccgg cggtagtcgg atttcttcgg gcggcccccc
     2041 tcgaagacca ccatcgaggc gaccgtgtgc tgaccgccga ggttcgagat gtcgaagccc
     2101 tcgatcctga ccggcagctc atccatgccc agggcatcgc cgagctggct cagggcctcg
     2161 atccttcgct gctgccggtg ctcgctctga agccggtcct ggtccagggc cagcttcgcg
     2221 ttccgctccg ccatctcgcg aagccggcgg gtatcgcccc gttcggcgat cctcacctcg
     2281 gttgcccggt tcgcccgccg cccggccagg aaggtctcga cctcggccag cttctccgac
     2341 atgtaggggc cgagcacgac ccgcttcggc accgccggcg aggccccgta gtactgctcg
     2401 atgaactcgg cggtgacctc gtcgatccct cgctcggccg gattctcgag gtagaaggac
     2461 tggcgctcgg cgaggatccc gtcgcggacc tgaaagacct gggcgttggc atcggtcccc
     2521 tcggtcgcga cgccgatcag atcagcggtg ccaaccgtgg agccggagat ccgctgtcgt
     2581 tcgaacagcg actggaccga ggccagccgg tcgcgaaaga ctgccgcagc ttcgaaatcc
     2641 tgggcggccg ccgcctcctt catgcgggtg tcgaggtccc gctcgatctg gtcgtaacgg
     2701 cccgaaagaa agtcgatcac ccggtcgatg ttggcccggt agtcggcctg gcttatgtag
     2761 tcgacgcagg gagcgccaca gcgcttgatg aagtagtcga ggcaggggct gcctgacctg
     2821 cggcccggtt ctttgccttc gcaggtccgg tactggaaga gcttgccgag caggtcgagc
     2881 gtgtcccgca cccggcgggc cgaggaaaac ggaccgaagt aggcccggcc tggccggtgc
     2941 cgctcccggg tgaagtagac ccgcgggtaa tcctcgtcga ggctgaccgc gacataggga
     3001 taggacttgt cgtcccgcaa cttgatgttg aagcggggcc ggtagcgctt gatgaagctc
     3061 tgttccgcaa tcagggcctc ggattccgat tcggtggcga gaaactcgat tcgctcgatc
     3121 agcctggtca tcccggcgtt gccgccgctg aaatgcgagg cgacgcgctt gcggatcgag
     3181 actgccttgc cgacgtagat cacttcgtcg tcgcggtcgc ggaacaggta aacccccggc
     3241 acatccggca gcgagcggcg ctcggcaacg atccgcgcct cgcggtccgg ttcctttgat
     3301 tgctgtttgc ctgtggattc ggccacaagc gaagggtaag acccaagggg gttgggatac
     3361 gctggcgcca cctatggccg ctgaaacgct cactcccccg gaactgaaaa ccatcgacat
     3421 cgcgatcgac ggcgagatcg ggacgatgac cttgaaccgt ccggagacct tcaacgctat
     3481 gagtcccgac ctgatcgagg acctggccac ggctttcgcc tggcttgccg accaggcccc
     3541 gctgcgcgga ctgatcatca ccggagccgg caaggccttc tgcgccggcg gcgacctcaa
     3601 ctggttcaag aagggccagg aagacccgga caccgacatc gtcgcctcgg tccggcgcgg
     3661 ggccgagctg cttcattccg gcatcgtcga cctgcagcgg atcccctacc cggtgatcgc
     3721 cgccctcaac ggcccgaccg ccggggccgg cttctcgctt gccctggcct gcgacatccg
     3781 gatctgttcc gaggacgcct ttctcgcctg tgcttacggc cggatcgggg tttcgccgga
     3841 cggcggcatg acctatttcc tgccccgggt ggtcggcccc tcgaaggcga tcgagatcct
     3901 gctcaatgac ccgaatctct cccctgccga cgcgctcgag gccggtctcg tctcagaggt
     3961 cgtggcaccg gaccagttgc tcgaccgggc ccgcgaaaag gccgacaagc tggcgaagaa
     4021 gtccccctac tacgtgaaga tggccaagca cctggtccat cagagtctcg acaacagcat
     4081 caccgagcaa ctccagctgg agcgtcacgg catcgccaac ggcatgggca ccgaagactc
     4141 gaagaacgcg atcgaggcct tcctcaccgg cggaaccgcc gaattcaaag gcgagtagcc
     4201 gccggccggg gccgggcatc gggctttttg gcaggtcagg ctttgagggc gcgggtcacg
     4261 ctctcgccga cgaagcaggg cttctcgccg atgcttccat cgggggcctg gacctcgagg
     4321 gtgaacctgg tgatcagttc cagccagccg ttgccgaggt cgttcgcctc ggtcagctcg
     4381 gccgaggcgc ggaccttcga gccggccggg accggcgccg gaaagcggac cttgttccag
     4441 ccgtagttca cgccgagggc gaaccccgac ttgacctcga tcaggtcgag ccggaaaccg
     4501 tcgaccaggg agagcgtgag gttgccgtgc gcgatcgtgg tgccaaaggg gctttcgttc
     4561 tttgccctct cgacctcgac gtggatccac tgatggtcac cggaaagatc ggcgaaggtg
     4621 tcgatcatct gctgggtgac ttcgcgccag ccggtcgggc cgaccgtctt gccaaccagt
     4681 tcctgcacct gctcggggcc ttcaatcgcg agagtttcca tggcgcaaca ttatcggcac
     4741 ctgttgcccc tcgggcaaca attccctgcc gctacctgac cagttcgatg gtcatgccga
     4801 tcggactgat cgctcccatg ccaccgagca tgccgagaat cttcgctaca taggcctggg
     4861 tttcgggata ggacgggatg cag
//
LOCUS       DAOQKN010000180         4776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_9910_length_4776_cov_5.354586, whole genome shotgun sequence.
ACCESSION   DAOQKN010000180 DAOQKN010000000
VERSION     DAOQKN010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9910_length_4776_cov_5.354586"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1623)
                     /locus_tag="PLJ64_10525"
     CDS             complement(<1..1623)
                     /locus_tag="PLJ64_10525"
                     /inference="COORDINATES: protein motif:HMM:NF017722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfotransferase family protein"
                     /protein_id="HNC93961.1"
                     /translation="METSENKLKFTPFNLAVILAAIFVGLGAGALIYRQHLIDKKDKA
                     EASGKSKPSNKDLNALAPVVGTESGNGDPGSKYDAQFSTTSMWPKFNPDRHYYVTRCV
                     PGRVMVQVRSNPEVKVKVWANPAESGRYAAEARPLPGQDFEVRIMPEGGQAESYEVRC
                     LPSNFPTWRYKRLRKPPKGMFMVSSYLTPGKTGRVWLIVFDQDGTPRWWYSPPYNTLG
                     GQVLYDGTVQFPRGFGDGFGQDARTATDIRDLDGNLVKTVRFDGAPTDGHEYYLLPNG
                     NQLVMSYDPRYGVDLRPIGGRADRGVLDGAFQEQTPDGRVLWSWNSGDHIPLTGTPHR
                     WWIREFNNPHFDRNGKIRYDHFHLNSIEPWGDQYVISTRHTDSIWGISKKTGDIVWKF
                     GGVKTPESLKVVGDDPYGNYPISGNHDARMVGDVLTVHDNGTHVEGRAPRALRYRIDL
                     KDRTATYIGAFTDKAAAPSSHCCGSFRPLGDGWVVSWGDNPWVDGFDSDGQLAFRLGL
                     PVPAYRAVPVPASVTEADLDSGLDAMEPDPPMS"
     gene            1765..2478
                     /locus_tag="PLJ64_10530"
     CDS             1765..2478
                     /locus_tag="PLJ64_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93962.1"
                     /translation="MTAPGKFSIRDAFRGMRLAFEQHRPLLIRVTVLLAALNALSNLF
                     DLTGAAPGFALAVGSFVLLGTVYQGLITALVCLPGRPEGIGELWQAVRPVLGVLIWVT
                     LVYSVVTVAGLFLLVIPGLVIFTIWSVAGQAVVVERKGLFSSLGRSTELISGRGPSVF
                     AFLLLLGIVALSISLLVIVATRPLGDGVLGSLVTPFLTNCLSAPFFVLGVASLYNELS
                     GRGVNPEAARPPDPGPDQP"
     gene            complement(2429..4024)
                     /locus_tag="PLJ64_10535"
     CDS             complement(2429..4024)
                     /locus_tag="PLJ64_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC93963.1"
                     /translation="MAEQKSGYLVLVAMIFAVSMTFIDQTIVSISIPEIQKDLSLTST
                     GVQWVINGYLLSLAALFALGGKLADVFGHRRLVVIGVLIFAVSSACCGFTPDSSIDEA
                     WLIFFRVIQGAGAALMFPAALAIVISSFPIRDRGKAMAIFFGVTGAFTSIGPLAGGYL
                     SEWTWRAIFWINIPVAILSLIFIWISKPEETKRPQPIDYRGAVLVCIGMALAVLGLQQ
                     SSTWGWGDPVTWICIVGGLLVLAFFIRFEMKQEHPLIRVAIFKDRAFAVDNVLLFLLS
                     VAFVPLFFFASMYAQLALGWETSEAGLYLLIFFAGFATAAQFGGRVLDSRGARPTVIL
                     GSLLASVGFVFWAMKLTDLAGGSNAQWPFIVLTGVGFGLLLGPVATDAVNRAPNTSYG
                     EATGITQTVRNYGASVGMAVLGTILILQNKSNIESELAGKVPARTASQIADEVSKAGS
                     GAVTSSAPSGGKAEEIYHSVQQAFAQSSKTVFLCMAGVMALCFVVALIGSPAGRMEEV
                     FADEGEAPKADPDRDPAAGPPRG"
     gene            4103..>4776
                     /locus_tag="PLJ64_10540"
     CDS             4103..>4776
                     /locus_tag="PLJ64_10540"
                     /EC_number="3.8.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HNC93964.1"
                     /translation="MEIVRTPEERFAQVPDFPWEPVYREWEGMRLAHVDEGEGEPVLL
                     VHGEPTWGFLWRKVMGPLLDAGYRCIVPDLPGFGRSDKPVDDEWYSYDRHTAALVSLI
                     EELDLSGLTLVCHDWGGPIGLRTASTEVPERFSRLVAMDTGLFTGYQKMSDNWNHFRD
                     FIASHRDVRVAMPIRGGCATELSDEVVAAYEAPFPDVDYKAGIRAFPPMIPVTPEDAG
                     AAEGQAA"
BASE COUNT          802 a         1531 c         1623 g          820 t
ORIGIN      
        1 gctcatcggc gggtcgggct ccatcgcgtc gaggccgctg tcgaggtcgg cttcggtgac
       61 cgaggccggg accggcaccg cgcgataggc cgggaccggc agaccgaggc ggaaggccag
      121 ctggccgtct gaatcgaatc cgtcgaccca ggggttgtca ccccaggaga cgacccagcc
      181 gtcacccagc gggcggaacg agccgcagca gtggctgctc ggcgctgccg ccttgtcggt
      241 gaaggcgccg atataggtcg cggtgcggtc cttgaggtcg atccggtagc ggagggcgcg
      301 aggggcacgg ccctcgacgt gagtgccgtt gtcgtgaacg gtcaggacgt caccgaccat
      361 gcgggcatcg tggttgccgg agatcgggta gttcccgtag gggtcgtcac cgaccacctt
      421 cagcgactcg ggagtcttga cgccgccgaa cttccagacg atgtcgccgg tcttcttgct
      481 gatcccccag atcgaatcgg tgtgacgggt cgagatcacg tactgatctc cccagggctc
      541 gatcgagttg aggtgaaaat ggtcgtagcg gatcttgccg ttgcgatcga aatgagggtt
      601 gttgaactcg cggatccacc agcggtgggg agtgccggtg agcggaatgt ggtcaccgga
      661 gttccaggac cagagcaccc tgccgtccgg agtctgctcc tggaaggcac catcgagcac
      721 gccgcggtcg gcacggccgc cgatcggccg caggtcgacc ccgtagcgcg ggtcgtagct
      781 catcaccagc tggttgccgt tcggcagcag gtagtactcg tggccgtcgg tcggggcgcc
      841 gtcgaagcgg acggtcttga ccaggttccc gtcgaggtcg cgaatgtcgg tggcggtgcg
      901 ggcgtcctgg ccgaagccgt caccgaaccc gcgggggaac tggaccgtgc cgtcgtagag
      961 cacctggccg ccgagggtgt tgtagggcgg gctgtaccac cagcgggggg tgccgtcctg
     1021 gtcgaaaacg atcagccaga cccggccggt cttgccgggg gtcaggtagg agctgaccat
     1081 gaacattccc ttgggcggct tgcgcaggcg cttgtaacgc caggtcggga agttggaggg
     1141 caggcaacgg acctcatatg actcggcctg accgccctcg ggcatgatcc tgacctcgaa
     1201 atcctggccg ggcaggggcc gggcctcggc cgcgtagcgt cccgactcgg cgggatttgc
     1261 ccagaccttg actttgactt ccggattcga gcggacctgg accatgaccc gccccggaac
     1321 gcagcgggtc acgtagtagt ggcggtcggg attgaacttc ggccacatcg aggtcgtgct
     1381 gaactgggcg tcgtacttcg agcccgggtc cccgttgccg ctttcggtgc cgaccaccgg
     1441 agcgagggcg ttcaggtcct tgttgctcgg cttgctcttg ccggaggctt ccgccttgtc
     1501 cttcttgtcg atcagatgct gacggtagat cagcgcaccg gcacccaggc ccacgaagat
     1561 cgcggccaga atcaccgcga gattgaacgg cgtgaatttg agtttgttct cggaggtttc
     1621 catgccgggg agggtttccg gttcgatccc gttcggcccg gtgacggcga cacgatcctc
     1681 gcgtgtgtca tcccggccac gcctgggcag ctctctgggg tccgggaact ataaaggcac
     1741 tccctgcggg tagggtgggc gcccatgacc gctcccggca agttctcgat ccgcgatgcc
     1801 ttcaggggaa tgaggctggc attcgagcaa caccgtccgc tgctgatccg ggtgaccgtt
     1861 ctgctggccg cgctgaacgc gctgtccaac ctcttcgacc tgaccggcgc tgcgcccgga
     1921 ttcgccctcg cggtcgggag cttcgtcctt ttgggcacgg tttaccaggg cctgatcacc
     1981 gctctcgtgt gcctgcccgg gcgacccgag gggatcgggg aactctggca ggcggtgcgc
     2041 cccgttcttg gcgtgctgat ctgggtcacg ctcgtctatt cggtggtgac cgtggccggg
     2101 ctcttcctgc tggtcatccc cggcctcgtg atcttcacga tctggtcggt tgccggtcag
     2161 gcggtcgtgg tcgagcggaa gggcctcttt tcatcgcttg gacgcagcac ggaactgatc
     2221 agcggcaggg ggccctccgt gttcgcgttc cttctcttgc tgggcatcgt cgccctttcg
     2281 atttcgctgc tggtgatcgt ggcaacccgc ccgctcggcg acggcgtgct ggggagcctg
     2341 gtcaccccgt tcctcaccaa ctgcctttcc gccccgttct tcgtgctcgg ggtggcctcg
     2401 ctctacaacg agttgagcgg gcggggcgtc aaccccgagg cggcccggcc gccggatccc
     2461 ggtccggatc agccttaggc gcttcaccct cgtcggcgaa gacctcctcc atccggccgg
     2521 ccggcgagcc gatcagggcg accacgaaac agagcgccat caccccggcc atgcagagaa
     2581 agacggtctt gctggactgc gcgaaagcct gctggacgct gtggtagatc tcctcggcct
     2641 tgcccccgga gggcgcgctc gaggtgaccg caccgctgcc ggccttcgag acctcgtcgg
     2701 cgatctggct ggccgtcctg gccgggacct tgccggccag ctccgactcg atgttggact
     2761 tgttctggag gatcaggatc gtgccgagga cggccatgcc gacactcgcc ccgtaattcc
     2821 tgaccgtctg ggtgatgccg gtcgcctcgc cgtagctggt gttcggcgcg cggttgaccg
     2881 catcggtggc gaccggcccg agcagcaggc cgaagccgac gccggtcagc acgatgaacg
     2941 gccactgcgc gttggagcca ccggccagat cggtcagctt catcgcccag aagacgaaac
     3001 cgaccgaggc gagcaggctg ccgagaatca cggtcggccg ggccccgcgc gagtcaagga
     3061 cgcgaccgcc gaactgggcc gcggtggcga aaccggcgaa gaagatcagc aggtagagcc
     3121 cggcttccga ggtctcccag ccgagcgcta gctgcgcgta catgctggcg aagaagaaga
     3181 gcgggacgaa ggcgaccgaa agcaggaaga gcagcacgtt gtccaccgcg aaggcccggt
     3241 ccttgaagat cgccacccgg atcagggggt gttcctgctt catctcgaag cggatgaaga
     3301 aggcgaggac gagcaggccg ccgacgatgc agatccaggt gaccggatca ccccagcccc
     3361 aggtcgaaga ctgctgcagc ccgagcacgg ccagtgccat gccgatgcag acgaggaccg
     3421 cgccccggta atcgatcggc tgcgggcgct tcgtctcctc cggcttcgag atccagatga
     3481 agatcaggga aaggatcgcc acgggaatgt tgatccagaa gatcgcccgc caggtccatt
     3541 ccgagaggta gccgccggcc agcggaccga tcgaagtgaa cgctcccgtg acgccgaaga
     3601 agatcgccat cgccttgccg cggtcccgaa tcgggaagga ggagatcacg atcgccaggg
     3661 cggcggggaa catcagggcg gcaccggccc cctgaatgac gcggaagaag atcagccagg
     3721 cctcgtcgat gctgctgtcc ggcgtgaaac cgcaacaggc cgaggagacg gcgaagatca
     3781 ggacgccgat cacgaccaat cggcgatgac cgaagacgtc ggcgagcttg ccgccgagcg
     3841 cgaagagcgc ggccagcgac agcagatagc cgttgatcac ccactgaacc ccggttgaag
     3901 tgaggctcag gtccttctgg atctcaggga tcgagatcga gacgatcgtc tggtcgatga
     3961 aggtcatcga cacggcgaag atcattgcga ccaggacgag gtagccactc ttctgctctg
     4021 ccatctgacc ccaacttacc gaagcgaccc gtcgctcaaa caaagttgac acgcatcagc
     4081 ttctaccgga taggctgccc gcatggaaat cgttcgcaca cccgaagaac gcttcgcgca
     4141 ggttccggat ttcccctggg agccggtcta ccgggagtgg gagggcatgc ggctcgccca
     4201 tgtcgacgag ggggaaggcg agccggtgct gctggtccac ggcgagccga cctggggttt
     4261 tctctggcgc aaggtgatgg gccccctgct cgacgccggc taccgctgca tcgtgcctga
     4321 cctgccgggc ttcggacgct ccgacaagcc ggtcgatgac gagtggtaca gctacgaccg
     4381 tcacaccgcc gccctggtca gcctgatcga ggagctcgac ctgagtggcc tgaccctggt
     4441 ctgccacgac tggggcggcc cgatcggcct gcggaccgca tcgaccgagg ttcccgagcg
     4501 tttctcccgg ctggtcgcga tggacaccgg cctcttcacc ggctaccaga aaatgtcgga
     4561 caactggaat cacttccgcg acttcatcgc ttcgcaccgg gacgtacggg tggccatgcc
     4621 gatccggggc ggctgcgcga ccgagctttc ggacgaggta gtcgccgcct acgaagcacc
     4681 cttccccgat gtcgattaca aggccgggat cagggccttc ccgccgatga tcccggtcac
     4741 gcccgaggac gccggcgcgg ccgagggtca ggccgc
//
LOCUS       DAOQKN010000181         4737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_10032_length_4737_cov_5.294105, whole genome shotgun sequence.
ACCESSION   DAOQKN010000181 DAOQKN010000000
VERSION     DAOQKN010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4737
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10032_length_4737_cov_5.294105"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1794
                     /locus_tag="PLJ64_10545"
     CDS             <1..1794
                     /locus_tag="PLJ64_10545"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HNC93965.1"
                     /translation="LFVVLIVVMAWVTIGNPAQLSTLRGETIASALFFSNWWFIFQDV
                     SYFEQFGPPSPLGHIWSLGVEEQFYIFWPWILLAGLAVFGVGQKARRGVRQRVQPRLA
                     LATLGLALVSIILMAVLFTPGPDSTRAYEGTDTRAFGLLIGAALAMVWPSRRLVGKVS
                     LQARNTLDLVGAVGLVSILILIATTDEFSPFIYRGGLVLLSIATAMVVASVAHPSSRM
                     GRLLGFRPLRWIGVRSYGIYLWQSPIILLTTPALAGFSLPRAVIQVGATFLIAALSWK
                     FIEDPIRHGAIGKFMKKWRDRRIRLRKPVTPEGWGGTVAVGLIFFFALLGFAGANPKN
                     QLLPIAVTNNDPLAELGTMTVSLESEGGAGPVPSQVAGDPTKTACTSVAYVGESTSLG
                     MVESSILPNPAERLDARLKAVGATDQHIDIAGSRSVDASDPGTLSGLDAAQAIRDSGF
                     KGCWVFALAVNDSGLVAADPSGSASSRIDQMLAVADGDPVLWVNGSIRESGTLGYMKP
                     EIGDWNEALVETCQAHPEMRVYDWDEEVDPDWFEYDGVHYTPTGYSQRSRLTADALVA
                     AFPGDKPLPSGQEAGDPESCLVGTGQNTTPE"
     gene            complement(1799..2671)
                     /locus_tag="PLJ64_10550"
     CDS             complement(1799..2671)
                     /locus_tag="PLJ64_10550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNC93966.1"
                     /translation="MQPRLTIAIPIHAGAKWIDGILDTVGKAPEWSRIVISDATHRDD
                     AVGRLAETLGDDRRVEVKSRPEKLDWIRHANLLLAEAETPFFCWMPQDDMVSPDYFEL
                     LVSALDEYPDRVLAFPTVVRKVTKGRLRTRAVGEIPYPSPPIELGEARPEEEAVRLLN
                     EWNLALGCWRGVFRTSVARPAPATVDCADLVWAFSMALAGNFIEVEEARYLKRFHRGS
                     ALHSMRWQGMSSAMNLYRVEVEARLGTNPARVDPVMAGVQRYLRFKHRLIMYTLRLRP
                     LAALALDRPRPALE"
     gene            complement(2671..3867)
                     /locus_tag="PLJ64_10555"
     CDS             complement(2671..3867)
                     /locus_tag="PLJ64_10555"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC93967.1"
                     /translation="MEKGRGETVIIGGGIHGVSTALALASRGRKSLILDAAAPLEGAS
                     VHNEGKVHLGFVYALDEHGATTRSMVEGALAFAPLIERWCGEVDWAGLRSSPFAYVVM
                     NEGLAGAGQLEAHYESVLAEIGSAGASFGSNYLGVDPGRAEIVRHDGVFPGMAAGHSQ
                     CWFETPERSVRPVALAALLKRAAVDERAIEILPGHRVTRARRREAGFELEVETPEGTR
                     SIGAETVVNCAWEGRPALDRMILDQPSATNFRVKYSVIIHGEDTGKVGTATLAQGPFG
                     DVLPWPGGDIYISWYPAARTHFGEEPLEELEPDRTVAVAVQDGIGELFPRLRGFHLKS
                     FAPCYILAEGRTDIGDRGSRLHSRKGAVFTESDGWWSNRSSKLTTAPLAGERCAASLT
                     GTEAAF"
     gene            complement(3918..4238)
                     /locus_tag="PLJ64_10560"
     CDS             complement(3918..4238)
                     /locus_tag="PLJ64_10560"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HNC93968.1"
                     /translation="MSEVVVVAVLEVKEGRAEEAITAFTPVIEATHREPGCVSYALHQ
                     DNSDPNTLVLVERWKSQDDLNSHFTQPHMAEMGSLAAELLTGQPRIVFCSGIPKGDPA
                     KGVL"
     gene            complement(4266..>4737)
                     /locus_tag="PLJ64_10565"
     CDS             complement(4266..>4737)
                     /locus_tag="PLJ64_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93969.1"
                     /translation="GRTTTLDRLLARYVADLHAIEAQGRQLCARTMGMVDHPELAGIL
                     NRQLRDGWVQQAVWKRDLEDLGDGPSTLKDLPLALGGNFVATFLLAQPDPNTKVAGFA
                     FAFQNLLAGSCEMLERVARLAGEDTIAAQAESRAAAVRNSANRISKLWEQLGGV"
BASE COUNT          757 a         1649 c         1489 g          842 t
ORIGIN      
        1 cttttcgtgg tcctgatcgt ggtcatggct tgggtgacga tcggcaaccc ggcccagctc
       61 tccacgctgc ggggcgaaac catcgcctcg gccctcttct tctccaactg gtggttcatc
      121 ttccaggacg tctcgtactt cgaacagttc ggcccgccct ccccgctcgg gcacatctgg
      181 tccctcgggg tggaggagca gttctacatc ttctggccgt ggatcctgct ggcgggactg
      241 gccgttttcg gggttggcca gaaggcacgg cgcggcgtgc ggcagcgggt ccagccccgg
      301 ctcgccctgg ccaccctcgg tctcgctctt gtctcgatca tcctgatggc ggtcctcttc
      361 acgcccggcc ccgactcgac cagggcctac gagggaaccg acacccgcgc cttcggcctc
      421 ctgatcggag ccgccctggc gatggtctgg ccgagccgga ggctggtcgg caaggtctcg
      481 cttcaggccc gaaacacgct cgacctggtt ggcgcggtcg gcctggtctc gattctcatc
      541 ctgatcgcga ccaccgacga gttctcgccc ttcatctacc ggggcgggct ggtgctgctc
      601 tcgatcgcga ccgcgatggt ggtcgcctcg gtcgcccatc cttccagccg gatgggccgc
      661 ctgctggggt tcaggccgct gcgctggatc ggagtccgtt cctacggcat ctatctctgg
      721 caatccccga tcattctgct gaccacgccc gccctggccg gcttttcgct gccccgtgcc
      781 gtgattcagg tcggggccac tttcctgatc gccgcgctct cctggaagtt catcgaggat
      841 ccgatccgcc acggggcgat cggcaagttc atgaagaagt ggcgggaccg gaggattcgc
      901 ctgcgaaagc cggtgacccc ggagggctgg ggcgggaccg ttgcggtagg gctgatcttc
      961 ttcttcgccc tgctcggatt tgccggggcc aacccgaaaa accagctcct gccgattgcc
     1021 gtgaccaata acgatccgct ggccgagctc ggcacgatga ccgtctccct ggagtccgaa
     1081 ggaggcgcgg gccccgtccc gagccaggtt gcgggagacc cgaccaagac cgcctgcact
     1141 tcggtcgcct atgtcggcga gtccacttca ctgggaatgg tcgagtcgtc gatcctgccc
     1201 aaccccgccg aaaggctcga cgcccgcctg aaagcggtcg gagcaaccga ccagcacatt
     1261 gacatcgccg gttcacgctc ggtcgacgct tcggaccccg gcacgctcag cggcctcgac
     1321 gctgcccagg cgatccgcga ctccggtttc aagggctgct gggtgttcgc gctggcagtc
     1381 aacgattccg ggctggtcgc cgccgatcct tccggcagcg cttccagccg gatcgaccag
     1441 atgctggccg tcgccgacgg cgatccggtg ctctgggtga acggttcgat ccgcgaaagc
     1501 ggaaccctgg gctacatgaa gccggagatc ggggactgga acgaggctct ggtcgagacc
     1561 tgtcaggccc atcctgaaat gagggtgtac gactgggacg aagaagtgga cccggactgg
     1621 ttcgaatacg acggggtcca ctacaccccg accggttaca gccagcgcag ccgcctcacc
     1681 gccgacgccc tcgtcgccgc tttccccgga gacaagcccc ttccctcggg ccaggaggcc
     1741 ggcgaccccg aaagctgcct cgtgggcact ggccagaaca cgacgcccga atagggcgct
     1801 actcgagcgc cggtctcggg cggtcgagtg ccagggccgc cagcggccgc agtcgcaggg
     1861 tgtacatgat caaccggtgt ttgaagcgga ggtatcgctg gaccccggcc atcaccggat
     1921 cgaccctggc cgggttggtg ccaagccggg cctccacctc gacccggtag agattcatcg
     1981 cgctcgacat gccctgccac cgcatcgagt gaagggcgct cccgcggtgg aaacgcttca
     2041 ggtagcgggc ttcctcgacc tcgatgaagt tcccggccag agccatcgag aaggcccaga
     2101 ccagatcggc gcaatccacc gttgccgggg ccggccgcgc caccgaagtc ctgaacactc
     2161 cccgccagca cccgagcgcc aggttccatt cgttcagcag ccgcaccgct tcttcctcag
     2221 gcctggcctc cccgagttcg atcggcgggg acgggtacgg aatttcaccc accgcacgcg
     2281 ttcgcaagcg gcccttcgtc actttcctga ccacggtggg aaaggccagg acccggtcgg
     2341 ggtactcgtc cagggcggaa accagcagct cgaagtagtc cggcgagacc atgtcatcct
     2401 gcggcatcca gcaaaagaac ggggtttccg cttcggcgag caggaggttc gcgtgccgga
     2461 tccagtccag cttctccggc cgcgacttga cctcgacccg gcggtcgtcc ccgagcgttt
     2521 cggccagccg accgaccgcg tcatcccgat gggtggcgtc gctgatcacg atgcggctcc
     2581 attccggcgc cttgccgacc gtgtcaagga tcccgtcaat ccatttcgcg ccggcgtgga
     2641 tcggtatcgc gatggtcagg cgaggctgca tcagaaggcg gcttccgtac cggtcaggct
     2701 ggccgcgcac ctttcacccg ccagcggcgc ggtagtcagc ttcgaggatc ggttcgacca
     2761 ccagccgtcg gactcagtga agaccgctcc cttcctcgaa tgcagccggc ttccccggtc
     2821 gccgatgtcg gtgcggccct cggccaggat gtagcagggc gcgaaactct tcaggtggaa
     2881 accccgcagt cgggggaaga gctccccgat cccgtcctga acggcgaccg cgacggtccg
     2941 gtccggttcg agttcttcca gcggttcctc accgaagtgg gtcctcgcgg ccggatacca
     3001 gctgatgtaa atgtcgccgc cgggccaggg aaggacgtca ccgaagggac cctgcgcgag
     3061 ggtggccgtc ccgacctttc cggtgtcttc cccgtggatg atcaccgagt acttgacccg
     3121 gaagttcgtg gccgaaggct ggtcgagaat catccggtcc agggccggcc ggccttccca
     3181 ggcgcagttc accaccgtct ctgctccgat cgaccgggtc ccttcaggcg tttccacctc
     3241 gagttcaaat ccggcttccc gccgccgcgc ccgggtgacc ctgtgtcccg ggaggatttc
     3301 gatcgcccgt tcatcaaccg ccgcccgttt caggagagcg gccagcgcaa caggacgcac
     3361 cgacctttcc ggagtttcaa accagcactg cgagtgaccc gcggccatcc cggggaaaac
     3421 cccgtcgtga cgaacgatct ccgcccggcc gggatcgacc cccaggtagt tgcttccgaa
     3481 ggaagctcca gcgctcccga tctcggccag caccgattcg taatgggctt cgagctgacc
     3541 ggctccggcc aggccttcgt tcatgaccac atatgcgaac gggctcgaac gcaggcccgc
     3601 ccagtcgact tcaccgcacc acctctcgat caacggagcg aaggcgagcg ctccttcgac
     3661 catcgagcgg gtggtggccc cgtgctcgtc gagggcatag acaaacccga ggtggacctt
     3721 cccttcgttg tgcacactcg cgccttcgag aggcgccgcc gcatccagga tcagtgactt
     3781 ccggcccctg gaggccagcg ccagcgcggt cgagaccccg tgaatcccgc ccccgatgat
     3841 caccgtttcc cctctcccct tctccactgc cgccagcgta ttccttcccg gccgggtgcc
     3901 aaggggcctg agcgaggcta gagaaccccc ttggcgggat ctcccttcgg gattccggag
     3961 cagaagacga tccgcggttg cccggtgagc agttcggcgg caagcgatcc catctcggcc
     4021 atgtgcggct gggtgaagtg tgaattcagg tcgtcctggg acttccagcg ttcaaccagg
     4081 accagggtgt tcgggtccga gttgtcctga tgaagggcat acgaaacgca gccgggttcc
     4141 cggtgcgtcg cttcgatcac cggggtaaac gcggtgatcg cctcctccgc ccttccctcc
     4201 ttgacttcca gtaccgccac cacgaccact tcgctcatga cccgacccta tccgggaatc
     4261 gacggtcaga cccctccgag ctgctcccaa agtttcgaga tccggttcgc cgagttcctg
     4321 acggcagcgg cacgcgactc ggcctgggcg gcgatcgtgt cctcgccggc gagtcgcgcg
     4381 acccgctcga gcatctcgca ggatccagcc agcaggttct ggaaggcgaa cgcgaacccg
     4441 gctaccttcg tgttcgggtc gggctgagcg aggaggaagg tcgcgacgaa gttgccgccg
     4501 agcgcgagcg gcaggtcctt gagggtggac ggtccgtcac cgagatcctc gagatccctt
     4561 ttccacacgg cctgctggac ccagccgtcc cggagctgac ggttgaggat gccggccagt
     4621 tccggatggt cgaccatccc catggtcctg gcacaaagct gccggccctg ggcttcgatc
     4681 gcgtgaaggt cggctacgta gcgggcgagc aggcgatcga gcgtggtggt ccgtccg
//
LOCUS       DAOQKN010000182         4713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_10113_length_4713_cov_4.248390, whole genome shotgun sequence.
ACCESSION   DAOQKN010000182 DAOQKN010000000
VERSION     DAOQKN010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4713
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10113_length_4713_cov_4.248390"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..1183)
                     /locus_tag="PLJ64_10570"
     CDS             complement(29..1183)
                     /locus_tag="PLJ64_10570"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNC93970.1"
                     /translation="MSRTLEIRNLAGMVTGRLGDDVAGPEVIRVEDGLITGFEAGPEP
                     DLVLDAGGAVVCPGLIDSHAHVVFGDWTPRQGTLGWIESSMHGGVTSMMSASEVHLPG
                     RPKDREGVKALAVAAQRAFENFRPGGVKVMAGSVIIEPTLQPEDFAELKENGVWLAKV
                     GFGDFSPQADAAPLVRAAQDNGFVVMNHTGGASIPDSSPVTIEDVLALGCDIIGHANG
                     GTTALPDGDLPRLFDAPGVIQLVQAGNLRSSMEIIEIAWDRDLLEKVVLATDTPTGTG
                     VMPLGMIKTVCEISSLCGMAAPKALALATGNNGAVLGVEQGVLEVGRPADLFCCFRPL
                     GSSQAGPLESIEYGDIPGIAAVVIDGEVRALRSRNTPAPDGACEIKEKNG"
     gene            complement(1242..1820)
                     /locus_tag="PLJ64_10575"
     CDS             complement(1242..1820)
                     /locus_tag="PLJ64_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid synthesis family protein"
                     /protein_id="HNC93971.1"
                     /translation="MSELNVDVEVRKIVTVVEEVHTEGGRADGGGPLKKAAVAVVFKN
                     PYAGKPFTEDLSELVEASAGLGTLIGERANEAIGGEVESHGKAVVVGTAGEQEHGNAA
                     KTTAFGNPFREGFGGGKAWLPSTTKRCAPGASVDVPLCYKDEIWVRSHYDTITIQVED
                     APAPDEIMLIGAVASRGRLNARVGGKSKEEVE"
     gene            complement(1817..2575)
                     /locus_tag="PLJ64_10580"
     CDS             complement(1817..2575)
                     /locus_tag="PLJ64_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HNC93972.1"
                     /translation="MANVWDDVLPEEDRQVFEAAGWGKDVGFGEKPVLLVVDVIYNFV
                     GDVPEPILESIKRWRYSCGERGWEGVKHLERLISAAREKQIPIVYTGMDRRPDGFDQG
                     AWNWKSYRSGDASDIKGSLGNEIVKEIAPEPQDIYFVKDKPSAFHGTHLLDYLIYLGA
                     DTVITTGTTTSGCVRASAVDATQYNYRSIVPEECVWDRSMISHKVNLLDIQMKYGDVK
                     KTDEVIEYFRSLPDRPCGPNTPTGKANTDQEVPK"
     gene            complement(2577..4133)
                     /locus_tag="PLJ64_10585"
     CDS             complement(2577..4133)
                     /locus_tag="PLJ64_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNC93973.1"
                     /translation="MSELQEVDLVIKGGTVVSPSGTRKVGIAVDDGKIVAVTEDRFLP
                     PARREVDATGLHVIPGLVDTEAHPGCYSPLKDDLGSESRAAVTAGVTTWGIHAPSTRM
                     GHPTFAEFVQKEDVISFHDSFPYFEQAVKDDSAVDVFATYMLETDQQASEIPEYAKDH
                     GVTSYKLYLQAMSPESEPNWPGRRAGLGAGFDDGVIWVTMENVAAMGRPGIVCMHCEN
                     WEVARIFDKRLKAEGRTDWATWSDRSPHYLEASHIRWYGDMAAHLGCPIYIQHSTTPE
                     SYKEILELRGRGVECHAQTGPHWLHFGKEEHNAWRINVPLRSRHNNPNIWSAIQAGII
                     NSVGSDHVVAWGDASYEASYNENIWELKTGFTSRVEMLLPVLLNGVNEGKITLERMVE
                     VACSEPARIFGVYPRKGLIDIGADADLVLVDLDKEVTVKNENVLTRSGWTCIMDHTLK
                     GWGVATFLRGKQMSKWDDGAPGPEFIGDADGQYLRRTPGQDLLPVAGGEGARAEVRPQ
                     VKIGMNDEGA"
     gene            complement(4235..>4713)
                     /locus_tag="PLJ64_10590"
     CDS             complement(4235..>4713)
                     /locus_tag="PLJ64_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93974.1"
                     /translation="GEGLESSLLPIEHRGIGCLWREDATVSRTAKGYSWGDNERAAYV
                     EVHDRLSALIDQVLTRLSARYEAILAYVGHTLTHRTFLASDEERREAGIPQNRRVGGE
                     TRQLIGVNDRSPGLVRLVPDAEGLEQIRAESGGRLPSGFLTGAPSLGQLELALRQF"
BASE COUNT          684 a         1604 c         1581 g          844 t
ORIGIN      
        1 ccgtgcctcg cgggccggga cgatcacttc agccattctt ctccttgatt tcacatgctc
       61 cgtcgggagc cggggtgttc cgggacctga gcgcacgcac ttccccgtcg atcacgaccg
      121 ccgcgattcc ggggatgtct ccgtactcga tcgattccag cgggccggcc tgggaggagc
      181 caagcgggcg aaagcagcag aagaggtcag ccggacggcc cacctcgagc actccctgct
      241 cgaccccgag cacagccccg ttgttgccgg tcgccagggc gagcgccttc ggcgcggcca
      301 tgccgcagag tgacgagatc tcgcagaccg tcttgatcat gccgagcggc atgaccccgg
      361 tgccggtcgg ggtgtcggtc gccaggacca ccttctcaag caggtcacga tcccaggcga
      421 tctcgatgat ctccatgctg gagcgcaggt tgccggcctg gaccagctgg atcaccccgg
      481 gggcatcgaa gagtcggggc agatccccgt ccggcagcgc cgtcgtgccg ccgttggcgt
      541 ggccgatgat gtcgcagccg agggccagca cgtcctcgat cgtgaccggt gaggaatccg
      601 ggatcgaagc gccgccggtg tggttcatga ccacgaagcc gttgtcctgc gccgcccgga
      661 ccaggggcgc cgcgtcggcc tgcgggctga agtcgccgaa gccgaccttg gccagccaga
      721 ccccgttctc cttgagctcc gcgaagtctt ccggctgcag ggtcggctcg atgatcaccg
      781 agccggccat caccttcacg ccgccgggcc ggaagttctc gaaggctcgc tgggcggcga
      841 ccgccagagc cttcaccccc tcgcggtcct tcggccgccc gggcaggtgg acctcggagg
      901 ccgacatcat cgaggtgacg ccgccgtgca tcgaggattc gatccatccg agcgtcccct
      961 ggcggggcgt ccagtcgccg aagacgacgt gggcatgcga gtcgatcagc cccgggcaga
     1021 cgacggcccc gccggcgtcc agcacgagat cgggttccgg ccccgcctcg aagccggtga
     1081 tcagaccgtc ctcgacccgg atcacctccg gcccggcgac gtcgtcgccc agccggccgg
     1141 tgaccatgcc ggcgaggttg cggatctcga gggtccggct caccggccca cccccgccgc
     1201 ggcccgcgcc gcactcttca tgctggcccg ggccatggcc tctactcgac ctcttccttg
     1261 ctcttgccgc cgacccgggc gttgagccgg ccgcgggaag caaccgcccc gatcagcatg
     1321 atctcgtcgg gggcgggggc gtcctcgacc tggatcgtga tcgtgtcgta gtgcgagcgg
     1381 acccagatct cgtccttgta acagaggggg acgtccacgc tggcgcccgg ggcgcagcgc
     1441 ttggtggtcg aaggcagcca ggccttgccg ccgccgaagc cctcgcggaa gggattgccg
     1501 aaggcggtcg tcttggccgc gttgccgtgt tcctgctcgc ccgccgtgcc gaccacgacc
     1561 gccttgccgt ggctttcgac ctcgccgccg atcgcctcgt tggcccgttc gccgatcagg
     1621 gtgccgagcc cggccgaggc ttcgaccagc tcggaaagat cctcggtgaa gggcttgccc
     1681 gcgtaggggt tcttgaagac caccgcgacc gcggccttct tcaacggccc cccgccatcg
     1741 gcccggccgc cctcggtgtg aacctcttcg accacggtga cgatcttgcg gacttcgacg
     1801 tccacattca gctcgctcac ttcggcacct cctggtcggt gttggctttg ccggtcggtg
     1861 tgttcggtcc gcagggccga tccggcagcg atcggaagta ttcgatcact tcgtcggtct
     1921 tcttgacgtc gccgtacttc atctggatgt ccagcaggtt caccttatgc gagatcatcg
     1981 accgatccca gacgcattcc tcgggcacga tcgaccggta gttgtactgg gtcgcgtcga
     2041 ccgcgctggc ccggacgcag cccgaggtgg tcgtgccggt ggtgatcacc gtgtcggccc
     2101 cgaggtagat caggtagtcg agcaggtggg tgccgtggaa ggcgctcggc ttgtccttga
     2161 cgaagtagat gtcctgcggc tcgggcgcga tctccttcac gatctcgttg ccgagcgagc
     2221 ccttgatgtc cgaggcgtcg ccggagcggt agctcttcca gttccaggcg ccctggtcga
     2281 atccgtcggg gcgccggtcc atgccggtgt agacgatcgg gatctgcttc tcccgggccg
     2341 cggagatcag ccgttcgagg tgcttgacgc cctcccagcc gcgctcgccg caggagtagc
     2401 gccaacgctt gatcgactcg aggatcggct cgggcacgtc gccgacgaag ttgtagatca
     2461 cgtcaacgac cagcagcacc ggcttctcgc cgaagccgac gtccttgccc cagccggccg
     2521 cctcgaagac ctggcggtct tcctcgggga gtacgtcgtc ccagacgttc gccatctcag
     2581 gctccctcgt cgttcatgcc gatcttgacc tgcggccgga cctcggcccg ggcgccttcg
     2641 ccgccggcca ccggcagcag gtcctggccg ggggtgcggc gcaggtactg gccgtcggcg
     2701 tcgccgatga actcgggccc gggggcgccg tcgtcccact tcgacatctg cttgccgcgc
     2761 aggaaggtgg caacgcccca gcccttcagg gtgtggtcca tgatgcaggt ccagccggaa
     2821 cgggtcagca cgttctcgtt cttgaccgtg acctccttgt cgaggtcgac caggaccagg
     2881 tcggcgtcgg cgccgatgtc gatcaggccc ttccgcgggt agacgccgaa gatgcgggcc
     2941 ggctcggagc aggcgacctc gaccatccgc tcgagcgtga tcttgccctc gttgacgccg
     3001 ttcagcagca ccggcagcag catctcgacc cgcgaggtga agccggtctt gagctcccag
     3061 atgttctcgt tgtaggaggc ctcgtagctg gcgtcacccc aggcgaccac gtggtcggag
     3121 ccgaccgagt tgatgatccc ggcctggatc gcagaccaga tgttggggtt gttgtggcgc
     3181 gagcgcagcg gcacgttgat ccgccatgcg ttgtgctctt ccttgccgaa gtggagccag
     3241 tgcgggccgg tctgggcgtg gcactctacc ccgcggccgc gcagctcgag gatctccttg
     3301 taggactcgg gagtggtcga gtgctggatg tagatcgggc agccgaggtg ggcggccatg
     3361 tcgccgtacc agcggatgtg cgaggcctcg aggtaatgcg gcgagcggtc cgaccaggtg
     3421 gcccagtcgg tccggccctc agccttgagg cgcttgtcga agatccgggc gacttcccag
     3481 ttctcgcagt gcatgcagac gatgccgggg cggcccatcg cggccacgtt ctccatggtc
     3541 acccagatca cgccgtcgtc gaagccggcg ccgaggccgg cccggcggcc gggccagttg
     3601 ggctcggact cggggctcat cgcctggagg tagagcttgt aggaggtgac cccgtggtcc
     3661 ttcgcgtact cggggatctc ggaggcctgc tggtcggtct ccagcatgta ggtggcgaag
     3721 acgtcgaccg ccgaatcgtc cttgaccgcc tgctcgaagt aggggaagga gtcgtggaag
     3781 gagatcacgt cctccttctg gacgaactcg gcgaaggtcg ggtggcccat ccgagtcgag
     3841 ggggcgtgga tgccccaggt ggtgacgccg gcggtcacgg cggcgcggga ctcggagccg
     3901 agatcgtcct tgagcggcga gtagcagccg ggatgggctt cggtgtcgac caggccggga
     3961 atcacatgga gtccggtggc gtccacttca cgccgcgccg gaggcaggaa ccggtcctcg
     4021 gtgacggcga cgatcttgcc gtcatctacc gcgatgccga cctttcgcgt gccgctggga
     4081 ctgaccacgg tcccaccctt gatgaccaga tccacttcct gcaattcgct cattacttct
     4141 cctcctgtct cagcatggtt gctccaccac ccaaacggtt ctgttgttca gtttgctgaa
     4201 ccttgttcaa gattttgact atcgtagcac tcgttcaaaa ctggcgcaag gccaattcga
     4261 gttgacccag agacggcgct ccggtcagga agccggaagg cagacggccg ccgctctccg
     4321 cccggatctg ctccagccct tcggcatcgg ggaccagccg gaccagaccc gggctgcggt
     4381 cgttcacccc gatcaattgc cgagtctcgc cgcccacccg acggttctgg gggatccccg
     4441 cctcccggcg ctcctcgtcg ctggcgagga aggtccggtg ggtcagcgtg tggccgacgt
     4501 aggcgaggat cgcctcgtag cgggcggaca gcctggtcag gacctggtcg atcagggccg
     4561 aaagccggtc gtgcacctcg acgtaggcgg cccgctcgtt gtcgccccag gagtagccct
     4621 tcgccgtccg gctcacggtt gcgtcttcgc gccacaggca gccgatgccg cggtgctcga
     4681 ttggcagcag ggacgattcg agcccttcac cgc
//
LOCUS       DAOQKN010000183         4701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_10153_length_4701_cov_4.541972, whole genome shotgun sequence.
ACCESSION   DAOQKN010000183 DAOQKN010000000
VERSION     DAOQKN010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10153_length_4701_cov_4.541972"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PLJ64_10595"
     CDS             complement(<1..231)
                     /locus_tag="PLJ64_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93975.1"
                     /translation="MAAFGAAVTGAFLLLVGPVIGDGLNSSPDPAPVVIATTHPATSA
                     QVAGARGLASASTVNAGRKRRTRTVVRRVTITS"
     gene            complement(343..1092)
                     /locus_tag="PLJ64_10600"
     CDS             complement(343..1092)
                     /locus_tag="PLJ64_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNC93976.1"
                     /translation="MTGTDAKQRLDALERSITECRRCPRLVAWREAVAADPPKRHRGE
                     EYWARPVPGFGDPDARILIHGLAPAANGGNRTGRPFTGDRSGDWLFAAIHRAGLSNQP
                     ESIDRGDGLRLRDTFVNAAVRCAPPGNRPNPSERDNCAPWLKDELGLLKEVRVVVALG
                     GFAWESMLRTVRQLGGEVPAPKPKFGHEAETVLKAPASARWEGPARWILLGCFHPSPL
                     NTSTGRLSAGMLDDVFARARVLAGLGAPPIA"
     gene            1116..1331
                     /locus_tag="PLJ64_10605"
     CDS             1116..1331
                     /locus_tag="PLJ64_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNC93977.1"
                     /translation="MGFLPNIGPLELIIVLVIAVLILGPKRIPAAAKSVGQGMRNFKD
                     SLGGGSEDEKKYEKSKSELDEKTETKA"
     gene            1354..1932
                     /gene="pgsA"
                     /locus_tag="PLJ64_10610"
     CDS             1354..1932
                     /gene="pgsA"
                     /locus_tag="PLJ64_10610"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HNC93978.1"
                     /translation="MVPLNLPNVLTLLRILAVPVVVVALLDGTPNGDMLAAVVFALAA
                     LTDGLDGYIARSRDSVTTFGKLMDPLADKLLITAALISLVSLDRLPAWVAMVIIAREF
                     AITGLRSIAADRGVVIAASWMGKVKTVLQIAAVFALIIFNPAPLWADILVYLALAATV
                     ISGFDYFFGLRKRIEEQRTASEKKTVTDQAPQ"
     gene            complement(2002..2982)
                     /gene="mptA"
                     /locus_tag="PLJ64_10615"
     CDS             complement(2002..2982)
                     /gene="mptA"
                     /locus_tag="PLJ64_10615"
                     /EC_number="3.5.4.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase MptA"
                     /protein_id="HNC93979.1"
                     /translation="MNPEIALSPNAVDVQSSQPATRVSLSRVGVTGVEKVISIGEDGH
                     YHAELECYVDLSPDQAGAHMSRFEENVSEAIEEVVRSQSMRAEQLAARIAELVRVKQK
                     GLRAEVSINARYAQTMPAPVSGIENQEIYNLLGTAVASPEGTRTVTGVQAQGMTACPC
                     AQGMVADLARTRLRSKGYDEDQVEEIIEEVPIATHNQRGLGTLWIGCTEGGPDLDARD
                     MLRLVESSMSSEIYELMKRPDEMSVVVKAHSQPRFVEDCVREMIRQVLETYPGLADDA
                     FVMARQENLETIHRHNVVAERFGQIADLRREVEGENPSRRHLTMKEWLEA"
     gene            3102..4268
                     /locus_tag="PLJ64_10620"
     CDS             3102..4268
                     /locus_tag="PLJ64_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNC93980.1"
                     /translation="MEFETDELKALAASGRDRGYLTFDAVAEAVAELDLGKGDVEELN
                     SWLTEQGIDLISQEESELRGGDGDGRAPERNGSPVLDGTGGLDGSGPIESEQIPVVTN
                     GATVAAGEAGSDATLDSLRLYLRSIGQVDLLTAQQEVELAKRIERGDMEAKRQMVEAN
                     LRLVVSIAKGYLGRGLSFLDLIQEGSLGLIRAVEKFDYRRGYKFSTYATWWIRQAVTR
                     AVADKSRSIRIPVHMVEKLNRVHFVERQLVQDLGREPEPQEIAAELGWTVQEVRDVWR
                     VSQTPVSLEKPVGEGEETELVDLVKDENQMEPFDEASLNLRSEGIRRVLNSLPERERE
                     VLIMRYGLGGLNPRTLEECGEAFGVTRERVRQIETSTLRKIKSLPEAQGLREAG"
     gene            complement(4269..>4701)
                     /locus_tag="PLJ64_10625"
     CDS             complement(4269..>4701)
                     /locus_tag="PLJ64_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93981.1"
                     /translation="VLGIRSEPAETVPWTLDSAGSERIPKTVYVRFQGGDSGRETYQD
                     DIILDQTAPVLSRAISDCCAAGASSVSARGHRARITRIRLRAHDRTSGVRFVQTAVKK
                     KRPAKKTRYRSRITVHGAVPKFVRVQDRAGNFSRWKKIRRR"
BASE COUNT          789 a         1558 c         1538 g          816 t
ORIGIN      
        1 ggaggtgatc gtgacccggc gcaccaccgt ccgggtcctt cgcttcctgc cggcgttcac
       61 cgtcgaggcg ctggccaggc cgcgagcccc ggccacctgg gccgaagtcg cgggatgggt
      121 ggtggcgatc acgaccgggg ccggatcggg actgctgttc aacccgtcac cgattaccgg
      181 tccgacgagc aacaggaatg cccccgtcac ggcggcgcca aaagctgcca cgcgtcctgt
      241 cctcctcgcg gaggattctc ccttgccact cgcgagcatg cccccattct gtcaggccgc
      301 ggctccgcgt caagtatttg ctgccacgga cggggccggc gttcaggcga tcggaggtgc
      361 gccgaggccc gcaaggaccc gggcccgggc gaaaacatcg tcgagcatgc cggcactgag
      421 ccggccggtc gaggtgttga gcgggctggg gtgaaagcag ccgagcagga tccaccgggc
      481 cggcccttcc caccgggccg aggccggcgc cttgaggacg gtctcggcct cgtgcccgaa
      541 cttcggcttt ggtgccggca cctccccgcc gagttgccgg accgtcctca gcatcgactc
      601 ccaggcgaat ccgcccaggg ccacgaccac ccgcacttcc ttcagcaggc ccagctcgtc
      661 tttgagccag ggcgcgcagt tgtcgcgttc ggaggggttc ggcctgtttc cgggaggtgc
      721 gcagcggacc gcggcattca cgaaggtgtc ccgcaaccgc agtccgtccc cgcggtcgat
      781 gctttccggc tgattcgaaa gcccggcccg gtggatcgcc gcgaagagcc agtcgccgga
      841 gcggtccccg gtgaaggggc gaccggtgcg gttgccgccg tttgcggccg gcgccaggcc
      901 gtggatgagg atgcgggcat ccggatcacc gaacccggga accggcctgg cccagtactc
      961 ctcgccgcgg tgccgcttcg gcgggtcggc cgcgaccgct tcccgccagg cgaccagccg
     1021 cgggcaccgg cggcattcgg tgatcgatcg ttccaatgca tccagccgct gctttgcatc
     1081 cgtacccgtc atggtccgta cactaggagg tgaatatggg ttttctgccg aacataggcc
     1141 cgctcgagct gatcatcgtt ctggtgattg ccgtgctgat cctcggtccg aagcgcattc
     1201 cggccgccgc caagtcggtc ggccagggca tgcgcaactt caaggattcc ctgggcggtg
     1261 gcagcgagga cgagaagaag tacgagaagt ccaagtccga gctcgacgag aagaccgaga
     1321 ccaaggcctg agcaagaagg cggggccgat ccggtggtac cgctcaatct ccccaacgtc
     1381 ctgaccctgc tccgcatcct cgcggtgccg gtggtcgtgg tcgccctgct ggacggaacg
     1441 ccgaacggcg acatgctcgc cgcggtcgtc ttcgctttgg ccgccctgac cgacgggctc
     1501 gacgggtaca tagcccgttc ccgggattcg gtgaccacct tcggcaagct gatggatccg
     1561 ctggccgaca agctgctgat caccgccgcc ctgatctccc tcgtttcact cgaccggttg
     1621 ccggcctggg tggcgatggt gatcatcgcc cgtgagtttg cgatcaccgg cctgcgttcg
     1681 atcgccgccg accgcggcgt ggtgatcgcc gccagctgga tgggcaaggt caagacggtg
     1741 ctgcagatcg ccgcggtctt cgccctgatc attttcaacc cggccccgct ctgggccgac
     1801 atcctcgtct acctcgccct cgcggcaacg gtgatcagcg gcttcgacta cttcttcggc
     1861 ctgaggaagc gaatcgaaga gcagcgcact gcgtccgaga aaaagacagt cacggaccag
     1921 gcaccacagt aaaggcacca ccatcagatt cgtacagcga gaccatcatt tcgtggccac
     1981 gagcgaaacg aatctgatgc cttatgcctc cagccactcc ttcatcgtga ggtggcggcg
     2041 gctggggttc tcgccttcga cttccctgcg gaggtcggcg atctgaccga acctctcggc
     2101 gaccacgttg tggcggtgga tcgtctcgag gttctcctgc cgggccatca cgaaggcgtc
     2161 atcggccagg ccgggatagg tctccagcac ctggcggatc atctcgcgca cgcagtcctc
     2221 gacgaagcgg ggctggctgt gggccttgac cacgaccgac atctcgtcgg gccgcttcat
     2281 cagttcgtag atctcggagc tcattgagct ctcgaccagc cgcagcatgt cacgggcatc
     2341 gagatccggg ccgccttcgg tgcagccgat ccagagggtg ccgaggccgc gctggttgtg
     2401 cgtggcgatc ggaacctcct cgatgatctc ctcgacctgg tcctcgtcgt aacccttgct
     2461 ccggagccgg gtacgggcca ggtcggcgac catcccctgg gcgcaggggc aggcggtcat
     2521 gccctgcgcc tgcactccgg tcacggtgcg ggtcccttcc ggtgaagcga ccgcggtgcc
     2581 gagcaggttg tagatctcct ggttttcgat cccggagacc ggggccggca tggtctgcgc
     2641 gtagcgggca ttgatcgaaa cctcggccct gaggcccttc tgcttgaccc gcaccagttc
     2701 ggcgatccgg gcggcaagct gctcggcccg catcgactgg ctgcggacaa cctcctcgat
     2761 cgcctcggag acgttctcct cgaaccggga catatgcgcc ccggcctggt cggggctcag
     2821 gtccacgtag cactcgagtt cagcgtggta gtgaccgtct tcaccgatgc ttatgacctt
     2881 ctcgacaccg gtcactccga cccgggaaag gctcacccgg gtggcgggct gggaggactg
     2941 aacgtccacg gcattggggg aaagcgcgat ttcgggattc attttcctga gaacagccta
     3001 gtaaccagag tgagcggccg gttaagaaca ctccgtataa ttccgcaacc gcgcacgaac
     3061 tcagggtttc cattagtgga aattcgttac tgggggtagt tatggagttc gaaaccgacg
     3121 aattgaaggc actggccgct tctggcaggg atcgcggcta cctgaccttc gatgccgtcg
     3181 cggaagcggt tgcggagctg gatctgggca agggtgacgt cgaggaactc aattcctggc
     3241 tgaccgaaca gggcatcgac ctgatcagcc aggaggaatc cgagctgcgg ggcggcgacg
     3301 gggacggccg ggctccggag cgaaacggat cgcccgtgct tgacggcacc ggcgggctgg
     3361 acggctcggg cccgatcgaa tcggagcaga tcccggtcgt aaccaacggc gccaccgtcg
     3421 cggcgggtga agccggttcc gacgccaccc tcgattcgct ccgcctctac ctgcggtcga
     3481 tcggccaggt cgatctgctc accgcccagc aggaagtcga gctggccaag cggatcgagc
     3541 gcggtgacat ggaggcgaag cggcagatgg tcgaagccaa cctgcgcctg gtcgtctcga
     3601 tcgccaaggg ttacctcggc cgcggcctca gcttcctcga cctgatccag gaaggctcgc
     3661 tcggcctgat ccgcgcggtc gagaagttcg actatcgccg cggttacaag ttttcgactt
     3721 acgcgacctg gtggatccgc caggcggtga cccgcgcggt tgccgacaag tcccgttcga
     3781 tccgcatccc cgtccacatg gtcgagaagt tgaaccgggt ccacttcgtc gagcgccagc
     3841 tggtgcagga tctcggccgg gagccggaac cacaggagat cgcggccgaa ctcggctgga
     3901 cggtgcagga ggtccgcgat gtctggcgcg tctcgcagac cccggtctcg cttgagaagc
     3961 cggttggcga gggcgaggag acggaactgg ttgacctggt caaggacgaa aaccagatgg
     4021 agcccttcga cgaagcctca ctcaacctcc gcagcgaggg aattcgcagg gtgctgaaca
     4081 gcctgccgga acgcgaacgc gaggtgttga tcatgcgtta cggcctcggc ggcctgaatc
     4141 cccgcaccct ggaggaatgc ggcgaagcct ttggcgtcac ccgcgaaagg gtccgccaga
     4201 tcgaaacctc caccctgagg aagatcaagt cactgcccga agcccagggc ctccgcgaag
     4261 ccggctgatc agcgccgcct gatcttcttc cagcggctga agtttccggc ccggtcctgg
     4321 acccggacga acttcggcac cgcgccgtgc accgtgatcc tcgagcggta acgggtcttc
     4381 ttcgccggcc gtttcttctt caccgcggtc tggacgaacc tgaccccact ggtccggtca
     4441 tgggccctca acctgatcct ggttatccgg gcccggtgcc cgcgggccga aaccgacgaa
     4501 gccccggcag cacagcagtc actgatcgcc cgcgaaagaa ccggggcggt ctggtccagg
     4561 atgatgtcgt cctgataggt ctcccgaccg gagtcaccgc cctggaagcg cacgtaaacc
     4621 gtcttgggga tccgctcgga accggccgag tcgagggtcc agggcacggt ctcggccggt
     4681 tccgagcgga tccccaagac g
//
LOCUS       DAOQKN010000184         4628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_10427_length_4628_cov_5.698010, whole genome shotgun sequence.
ACCESSION   DAOQKN010000184 DAOQKN010000000
VERSION     DAOQKN010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4628
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10427_length_4628_cov_5.698010"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="PLJ64_10630"
     CDS             complement(<1..340)
                     /locus_tag="PLJ64_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93982.1"
                     /translation="MNTGRCCSNYTRSNFLRSAAAKAGSGLPGIEPGMPVPAGTGLTR
                     RGFIAGAAGLAVAVYAAGKTPLPLFEDGIAEAAQSDRILVSIFLDGGADTLSLLAPVG
                     HSRYHDLRPVL"
     gene            complement(337..1875)
                     /locus_tag="PLJ64_10635"
     CDS             complement(337..1875)
                     /locus_tag="PLJ64_10635"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HNC93983.1"
                     /translation="MAVKAGKSTRFRSYSRQRWVDSGEGVVRLKTKVIRRRRGEKAPR
                     KDPGPRPLPRPQIPRNPWKPQPFGTYHGEFGRLEATRLLNRAGFGPRPGQAEQLAKMG
                     MKKAVLSLTRPQGQAKLIGPEPVDGDGLPLAPFDSWGHDHLWWLDRMVRTDQPFRERM
                     ALVFHDWFATSNSGVSQQRQMIDQYWTLRAGVNGTFDDLVVQITKDPAMLQWLSGGDN
                     TKWSPNENYARELMELFTLGADRGAYTEDDIREAARALTGWTYDWDDDLGAINFRFDP
                     EYHDDETKKIFSSKGNWDWRDVCRLCVTHELHASFFVDKLWSYFVPAAPSKDTRQALI
                     RLYLKNDREILPVVEAILMHPDFYRGPSMVKPPVVFNASLLRALKLPVNTDAWAWLGE
                     GAGQQLFLPPNVAGWDDERWLDTSTVRARWLMVTYALDSRHFEAWDDSYREKESVREA
                     VNRALAAYGWPALRSDHQNELVSMAGRISPYIQEDWQRRPYLAMRQNALLQLIASCPD
                     FQLS"
     gene            2025..2306
                     /locus_tag="PLJ64_10640"
     CDS             2025..2306
                     /locus_tag="PLJ64_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93984.1"
                     /translation="MGYEYVFIALLFGIATGIIGRAKGGSFLIWLVVGTILPGLGLIA
                     VFMMRNEINEPERRCENCGKVLKLYVQVCPRCGYDLYLPPPEETRMPPR"
     gene            complement(2541..3605)
                     /locus_tag="PLJ64_10645"
     CDS             complement(2541..3605)
                     /locus_tag="PLJ64_10645"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNC93985.1"
                     /translation="MILASIFPAQLTALRATGLVIAVLIIAFAIIRRRELRNADVILL
                     LLAGIGLAIVSGTELTDRLLSALSFNRGNGGRILGLAVIAIFILFLMALRALSVGARN
                     ARQLSAVLEGISSEQFRVADNRKKFKGKVAIVIPAYNEADNIGYVLDQIPAEVCGLPT
                     ATLVVDDGSRDGTEEVAEAHGAVVIRHVVNRGGGAALRTGYRLMVDSEAAIVVTLDAD
                     GQHLPAEMPNLVQPVLDGKADVAHGSRVLGHAEDNHFAREMGIVFFNRLVSFITRTHV
                     TDCSNGYRAVRTSVLPTLVLKQEQFHTSEFMIEAIKRGVPAVEVPVTVEQRLHGHSKK
                     PAVFRYGMGFANAIVRTWLR"
     gene            complement(3602..>4628)
                     /locus_tag="PLJ64_10650"
     CDS             complement(3602..>4628)
                     /locus_tag="PLJ64_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93986.1"
                     /translation="AGLVAVYPPFIHSTGALMSEPPAFFMLPASVLAFLWADRRMGEA
                     TGSGASGGKGSLWSRVWPWLLPGLGFGLTALIRPEYLSVCAAFTIFLLIRAWMKSGAG
                     PALAASALFVLAVLVPIVPWTVHNYHELDRVVPLSTGSGKALFTGTYYPGDGEYQQVK
                     AELYFQQTGNRLDPGSAELEAVDPVPLFDRVANEYKAANNLPNLDRDDALGRLGKENL
                     KEYLGDHPVGYVGMTFRKVGRMWSSGVGDAMSSPIGRAIQILLVLAGLAGLALLAWRR
                     RWEAIAVGLPIATVTAVAAITLAPARRNEILMTLVLPLAAVALDALVNREPKPAGPAP
                     DAANETS"
BASE COUNT          782 a         1476 c         1607 g          763 t
ORIGIN      
        1 ccaggacggg gcgcaggtcg tgatatctcg agtgaccgac cggggcgagc agtgaaagcg
       61 tgtcggcgcc tccgtcgagg aagatcgaaa ccaggatccg gtctgactgg gcggcctcgg
      121 cgatcccgtc ttcaaagagc ggcagcgggg tcttgccggc cgcgtagacc gcgacggcca
      181 gaccggccgc gccggcaatg aaaccgcggc gggtcagtcc ggtcccggcc ggtaccggca
      241 tgcccggctc gataccgggg aggccggacc ccgcctttgc ggcggccgaa cggaggaagt
      301 tcgagcgggt gtagttgctg cagcagcgtc cggtgttcat gagagctgga agtccgggca
      361 ggaggcgatc aactggagca gggcgttctg gcgcatcgcg aggtaggggc gacgctgcca
      421 gtcttcctgg atgtaagggg agattcggcc cgccatcgag acgagctcgt tctggtggtc
      481 cgacctgagg gccggccatc cgtaggcggc gagtgcacga ttcacggcct cccggaccga
      541 ttccttttca cggtacgagt cgtcccatgc ctcgaagtgg cgggagtcga gcgcgtaggt
      601 gaccatcagc cagcgggccc tgaccgtcga agtgtcaagc cagcgctcgt cgtcccagcc
      661 ggccacgttc ggcggcagga aaagctgctg gcccgcgcct tcgccgagcc aggcccaggc
      721 gtcggtgttg accggcagct tcagcgcccg cagcaggctc gcattgaaga cgaccggcgg
      781 tttgaccatc gacgggccgc ggtagaagtc ggggtgcatg aggatcgcct cgaccaccgg
      841 caggatctcg cggtcgttct tcaggtacag ccggatcagg gcctgtcgcg tgtcctttga
      901 gggcgcggcc ggaacgaagt agctccagag cttgtcgacg aagaaactgg cgtggagttc
      961 atgggtgacg cagagccggc agacgtcccg ccagtcccag ttccccttgc tgctgaagat
     1021 cttcttggtc tcgtcgtcgt gatactcggg gtcgaagcgg aaattgatcg cgccgaggtc
     1081 atcgtcccag tcgtaggtcc agccggtcag ggcccgggcc gcctcgcgga tgtcgtcctc
     1141 ggtgtaggcg ccccggtcgg caccgagggt gaagagttcc atcagttctc gggcgtagtt
     1201 ctcgttcggt gaccacttgg tgttgtcgcc gccactcagc cactggagca tcgccggatc
     1261 cttggtgatc tgaacaacca ggtcatcgaa ggtcccgttc acgccggccc gcagggtcca
     1321 gtactggtcg atcatctggc gttgctggga gaccccggag ttcgaggttg cgaaccagtc
     1381 gtggaagacc agggccatcc gttcccggaa cggctgatcg gtccggacca tgcggtcgag
     1441 ccaccagagg tgatcgtgac cccaggaatc gaatggcgca agcggcaggc cgtcgccgtc
     1501 gaccggttcg ggcccgatca gcttcgcctg gccctgaggc cgggtcaggg aaaggaccgc
     1561 cttcttcatt cccatcttcg ccagctgctc cgcctgacct ggccgcgggc cgaagcccgc
     1621 ccggttcagc aggcgggtgg cttcgagccg accgaactct ccgtggtagg tgccgaacgg
     1681 ctgcggcttc caggggttgc ggggaatctg cggccggggc agcggccggg ggccgggatc
     1741 cttgcgggga gccttctcgc ccctgcggcg acggatcacc ttcgtcttca ggcggacgac
     1801 gccctcgccg gagtcgaccc agcgctggcg gctgtagctc ctgaatctcg tgctttttcc
     1861 tgcctttaca gccatcccct gcccaaacat tgttcaccaa ccgtcaagcg gaaccttctt
     1921 cgccccatcg ccggcgcccg tttccagacc agaaccgaac ggtctcgtcc catccacaac
     1981 ccgatcggcc gtcaagcgga gcggtacccc ggcacaatgt cggcgtgggt tacgaatacg
     2041 tcttcatcgc cctgctcttc ggaatcgcga ccgggatcat cggccgcgcc aagggcggct
     2101 ccttcctgat ctggctggtc gtcggcacga tcctgcccgg gctcggcctg atcgccgtct
     2161 tcatgatgcg aaacgagatc aacgaaccgg aacgccgctg cgagaactgc ggcaaggtcc
     2221 tcaagctcta cgtccaggtc tgcccccgct gcggctacga cctctacctg ccaccaccag
     2281 aagaaacccg aatgccgccc cggtaaggcg cggccaacgg cccgcagcgt tggcgtgggg
     2341 ctgaatcatc gagtgcccac tttgtgccgg tatgtggcgc aaaatgggat ctcgccgtcc
     2401 gctgaccggg ttggacccgg gtcgacgatt gaggtgccgc aggtttgggc ccgatccggt
     2461 ctgaaacctg cggcaccttc ggggctcgac cgggtcctgg cagccgtggc gtcggggacc
     2521 ccgttcgact tgcagttaag tcagcgaagc caggttcgga ctatggcgtt ggcgaagccc
     2581 attccgtagc ggaagacggc gggcttcttc gagtggccgt gcaggcgctg ttcgaccgtg
     2641 accgggacct cgaccgcggg cacgccccgc ttgatcgcct cgatcatgaa ctcggaagtg
     2701 tggaactgct cctgcttgag caccagggtg gggaggaccg aagttcgcac tgcccggtag
     2761 ccgttcgaac agtcggtgac gtgggtccgg gtgatgaagg agaccagccg gttgaagaag
     2821 acgatgccca tttcccgggc gaagtgattg tcctcggcgt gaccaagcac ccggctgcca
     2881 tgggcgacgt cggccttgcc gtcgaggacc ggctggacca ggttcggcat ctcggccggg
     2941 aggtgctggc cgtcggcgtc gagggtgacc acgatcgccg cttcggaatc gaccatcagc
     3001 cggtagccgg tgcgcagtgc ggcgccgcca ccgcggttta cgacatggcg gatcaccacc
     3061 gccccgtggg cttcggcgac ttcctcggtg ccgtcgcgtg aaccgtcgtc gacgaccagc
     3121 gtggcggtcg gaagtccgca gacctcggcc gggatctgat cgagcacgta tccgatgttg
     3181 tccgcctcgt tgtaggcggg gatcacgatc gccaccttgc ccttgaactt cttgcggttg
     3241 tcggcgaccc ggaactgctc ggaagagatg ccttcgagta cggccgaaag ctggcgggcg
     3301 tttctggctc cgaccgagag cgcccgcagc gccatcagga agaggatgaa gatcgcgatg
     3361 accgcgagac cgaggatccg gccgccgttg ccgcggttga aggagagggc ggagagcagg
     3421 cggtcggtga gttcggtgcc ggagacgatg gccaggccga ttccggccag gagcagcagg
     3481 atcacgtcgg cgttcctcag ctcgcgacgc ctgatgatcg cgaaggcgat gatcaggacc
     3541 gcgatcacga ggccggtcgc ccgcagggcg gtgagctggg cagggaatat cgaggcgagg
     3601 atcatgaggt ttcgtttgcg gcatcgggcg ccgggccggc cggcttcggc tcccggttga
     3661 ccagggcgtc gagcgcgacc gccgccagcg gcaggacgag cgtcatcagg atctcatttc
     3721 gccgggcggg agcgagtgtg atcgccgcga ctgcagtaac cgtggcaatc ggcaagccaa
     3781 ccgcgatcgc ctcccagcgc cggcgccagg ccagcaaagc caggccggcc agcccggcga
     3841 ggaccagcag gatctgaatc gcccggccga tcggcgagga catcgcgtcg ccaaccccgg
     3901 aactccacat ccggccgacc ttgcggaagg tcatgccgac atagccgacc gggtgatcgc
     3961 cgagatactc cttcaggttc tccttgccga gccggcccag ggcgtcgtcc cggtcgaggt
     4021 tcggcaggtt gttcgcggcc ttgtactcgt tggcgacccg gtcgaagagg ggaaccggat
     4081 cgaccgcttc cagctcggcc gaacccgggt caagccggtt gccggtctgc tgaaagtaga
     4141 gctcggcctt gacctgctgg tactcgccgt caccggggta gtaggtgccg gtgaacaagg
     4201 ccttgccgct gccggtggaa agcgggacga cgcggtcgag ctcgtggtag ttgtggaccg
     4261 tccagggaac gatcggaacc agcacggcga gcacgaagag cgccgaggcc gcaagcgccg
     4321 gtccggcccc cgacttcatc caggcccgga tcagcaggaa gatcgtgaag gcggcgcaga
     4381 ccgagaggta ctcggggcga atcagcgcgg tcaggccgaa accgaggccg ggcaaaagcc
     4441 agggccagac gcgactccac agcgaaccct ttccgccgct cgcgccgcta ccggtggctt
     4501 cgcccatcct gcggtcggcc cagagaaagg cgaggacgga ggccggcagc atgaagaagg
     4561 ccggcggctc gctcatcagc gccccggtcg agtggatgaa cgggggatag acggcgacca
     4621 ggccggcg
//
LOCUS       DAOQKN010000185         4624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_10449_length_4624_cov_5.534909, whole genome shotgun sequence.
ACCESSION   DAOQKN010000185 DAOQKN010000000
VERSION     DAOQKN010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4624
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10449_length_4624_cov_5.534909"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="PLJ64_10655"
     CDS             complement(<1..289)
                     /locus_tag="PLJ64_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93987.1"
                     /translation="MIAGTTGLEGSARGRMAILAGIIGAVLGYLGTSILDGAPVIGAL
                     VGAIAAVLSTAVVSDVIAGAQRRGGTAALAVITVFAALVIAIICLLFPFFAI"
     gene            361..1719
                     /locus_tag="PLJ64_10660"
     CDS             361..1719
                     /locus_tag="PLJ64_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNC93988.1"
                     /translation="MAASPPPRRQQTRAKDLERYASLFARRTRVMRSSAMRDMMEITA
                     RPEVISLAGGLPDTSTFPPEAFAAEMHRIEKTAVAEVLQYGPTEGSWLVREQIVEVMA
                     AEGTRVDALDITVTTGGQQGIDLITKTLIDPGDVIICDAPTYPGAIPTFCSYEADVRQ
                     IEMDADGMRVDLLEEMIAKLEAEGRKPKFIYSIPTFQNPGGVTLSLERRRRLVEIARE
                     KEILVVEDNPYGLLRFEGEPLPTLYSLDGGNFVVYLGTFSKILSAGLRIGWLVTPNPI
                     REKVVLGKQAADLCTSTLTQDFAGHFFKTGRWREYVAELTGIYRARRDVMISAMAEYF
                     PHGATWNKPQGGLFIWATMPDVLSSEDLLAKSLSRNVAFVPGPNAYVDGRGVSSMRLN
                     FSGVREDEIIEGIRRIGQVAREMLKLYETMTSTDEMKLPPPESTDGGGSEMKLPPRED
                     LP"
     gene            1716..2636
                     /locus_tag="PLJ64_10665"
     CDS             1716..2636
                     /locus_tag="PLJ64_10665"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNC93989.1"
                     /translation="MKVALLQGGRSLERGVSLRSGMRVTESLLDLGHEVDVMDLGPDL
                     VNRLEAKRPDVVFIALHGTEGEDGTIQALLELLGIPYTGPGVAACTRCMDKSLTKYIL
                     REHDLPTPDWVTYSEASIRDFGAASTFGAAVERVGLPLVAKPASQGSSLGVKVVTELE
                     QIPGALITALSYDNRALLEAWVNGRELAVSVLGGEALPVVEAIPTDSNAYDFEARYEI
                     GGASFECPADLPDADAAEVERVALAAYESLGCSGFARVDIMLGESGPQILEINAIPGL
                     TETSLLPQAAEAAGLDFNQMVQRILDLATQ"
     gene            complement(2715..3203)
                     /locus_tag="PLJ64_10670"
     CDS             complement(2715..3203)
                     /locus_tag="PLJ64_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HNC93990.1"
                     /translation="MREVQIYGVSFDMVGKQPIVLLKTIDDNRYLPIWIGHPEAAAIL
                     MKLQGADTPRPMTHDLIVDMLDQVETKVERISVNELRDNTFFATITLSVSGTEIEIDS
                     RPSDALALAVRLQAPIFVAEEVIEESSIEFDQGIEDNEQVVEKFKDFLDDVSPEDFLQ
                     DS"
     gene            complement(3362..3435)
                     /locus_tag="PLJ64_10675"
     tRNA            complement(3362..3435)
                     /locus_tag="PLJ64_10675"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3399..3401),aa:Ile,seq:gat)
     gene            complement(3523..4371)
                     /locus_tag="PLJ64_10680"
     CDS             complement(3523..4371)
                     /locus_tag="PLJ64_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNC93991.1"
                     /translation="MAERRGMGRGLGAILPESATGPDMRELPVKSIDRNPEQPRTNFS
                     EEALEALAESIRASGVVQPLIVRPAGRGKYEIIAGERRWRAAQMAGVEKVPVVIRDSD
                     EADRLEVALIENMVRENLNPVEEAKACAALVEDLGLSKEELGKRVGRSRPQISNLIRL
                     LDLPDDVLEMLEGGELSEGHGRAILQVPDHADRRKLAKQARKEGWSVRQTEAGARALA
                     AAPKKGGRKQGLSAEESEAVAEATERLETKLDRPVKIRPKGKGLVVEIELEDLAQAAE
                     IARRLK"
     gene            complement(4364..>4624)
                     /locus_tag="PLJ64_10685"
     CDS             complement(4364..>4624)
                     /locus_tag="PLJ64_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HNC93992.1"
                     /translation="VRREMNPTLELAGVLVTMHDERTRLAQDVERELRDHFSETVFRT
                     VIPRTVRVAEAPSYGIPVTDYSPSSRGSEAYLALAEEVMARG"
BASE COUNT          810 a         1527 c         1469 g          818 t
ORIGIN      
        1 tgatggcgaa gaacgggaag agcaggcaga tgatcgcgat caccagggcc gcgaagacgg
       61 tgatcactgc cagggctgcc gtgccgcccc gccgctgggc cccggcgatc acgtcggaaa
      121 cgacagcggt gctcagcacc gccgcaatcg cgccgaccag ggcaccgatg accggggcgc
      181 cgtccagaat cgaggtaccg aggtagccga ggaccgcgcc gatgatcccg gcgaggatcg
      241 ccatgcggcc ccgggcggaa ccttccaggc cggttgtgcc ggcgatcatt ccggtcgcaa
      301 tgccgattcc ggcggcgagg cagattgtcg agaaagtgtc gataccggca gaatagaagg
      361 atggcggcct caccacctcc ccggcgtcag cagacccggg ccaaagacct tgagcggtac
      421 gcctctcttt tcgccaggcg aacccgggtg atgcgctctt cggcgatgcg ggacatgatg
      481 gagatcaccg cccgtcccga ggtgatttcc ctcgccggcg gcctgccgga cacttccacc
      541 ttccccccgg aggccttcgc cgccgaaatg caccggatcg agaagaccgc ggtcgccgag
      601 gtgctccagt acgggccgac cgaaggatcc tggctggtcc gcgagcagat cgtcgaggtg
      661 atggcggcag agggaacccg ggtcgacgcg ctggacatca ccgtcaccac cgggggccag
      721 caggggatcg acctgatcac caagaccctg atcgaccccg gcgacgtgat catctgcgac
      781 gccccgacct accccggggc gattccgacc ttctgctcct acgaagccga cgtccggcag
      841 attgaaatgg acgccgacgg gatgcgggtc gacctgctcg aggagatgat cgcgaagctc
      901 gaggccgagg gccgcaagcc gaagttcatc tactcgattc ccacttttca gaatccgggc
      961 ggggtgacgc tctcgttgga acggcgccgg cggctggtcg agatcgcccg cgaaaaggag
     1021 atcctggtcg tcgaagacaa cccctacgga ctgctccgtt tcgagggcga acccctgccc
     1081 acgctctaca gcctcgacgg cggcaacttc gtggtctatc tcggcacttt ctcgaagatc
     1141 ctctcggccg gtctcaggat cggctggcta gtcactccca atccgatccg cgagaaggtc
     1201 gtgctcggca agcaggccgc cgatctctgc acttccaccc tgacccagga cttcgccggg
     1261 catttcttta agaccggccg ctggcgcgag tacgtggccg agctgaccgg catctaccgg
     1321 gcccggcgcg atgtgatgat cagcgccatg gccgagtatt tcccccacgg cgcgacctgg
     1381 aacaagccgc agggaggcct cttcatctgg gcgaccatgc ctgacgtgct gagcagcgag
     1441 gacctgctgg cgaagtcgct ttcgcgcaat gtcgctttcg tgcccgggcc gaatgcctac
     1501 gttgacggcc gcggggtcag ctcgatgcgg ctgaacttct ccggagtcag ggaagacgag
     1561 atcatcgagg ggatccgccg catcggccag gtagcccgcg agatgctgaa gctctacgag
     1621 acgatgactt caaccgatga aatgaagctt ccgccaccgg agtccactga tggaggcgga
     1681 agcgaaatga agcttccgcc acgagaggac cttccttgaa agtcgcgctg cttcagggcg
     1741 gccgctcgct cgagcggggc gtttcacttc gatccggaat gcgggttacc gaatccctgc
     1801 tcgacctcgg ccacgaggtg gatgtgatgg acctcggccc ggatctggtc aaccggctcg
     1861 aagcaaagcg tccggacgtg gtcttcatcg ccctgcacgg caccgagggc gaggacggca
     1921 cgatccaggc cctgctggaa ctgctcggca tcccctacac cgggcccggg gtcgccgcct
     1981 gcacacgctg catggacaag agcctgacca agtacatcct gcgcgagcat gatctgccca
     2041 ccccggactg ggtcacctac agcgaggcgt cgatccgtga tttcggtgcc gcctccactt
     2101 tcggtgcggc ggtggaacgg gtcgggctgc ccctggtcgc caagcctgct tcacagggat
     2161 catcgctcgg cgtcaaggtg gtgaccgagc tcgagcagat cccgggcgcc ctgatcaccg
     2221 cgctcagcta tgacaaccgg gcactgctgg aagcctgggt caacggccgc gaactggcgg
     2281 tgtcggtgct gggcggcgaa gccctgccgg ttgtcgaggc gatcccgacg gacagcaatg
     2341 cctacgactt cgaggcccgt tacgagatcg gcggagccag tttcgaatgc ccggccgatc
     2401 tccccgacgc cgacgccgcc gaggtcgagc gggtcgccct cgcagcctac gagtcactgg
     2461 gctgttccgg cttcgcccgg gtggacatca tgctcggcga atccggcccc cagatcctcg
     2521 agatcaacgc aatccccggc ctcaccgaaa cctccctcct cccccaggcc gccgaagccg
     2581 ccggcctcga cttcaaccag atggtccagc gaatcctcga cttggccacc cagtagagcc
     2641 cctcccaacc attgtggttg cgagtgggag gggcgggttg aacctggagg cggggcgaag
     2701 ctcgccgact gcagttaaga gtcctggagg aagtcttcgg gggagacgtc gtcgaggaag
     2761 tccttgaact tctcgaccac ctgctcattg tcttcgatgc cctggtcgaa ctcgatcgag
     2821 gattcctcga tcacttcctc ggccacaaag atcggtgcct ggagcctgac cgccagggcc
     2881 agcgcgtcgg aggggcgtga atcgatttca atctcggtgc ctgagaccga gagcgtgatc
     2941 gtggcgaaga aggtgttgtc gcgaagctcg ttgaccgaga tccgttccac cttggtttca
     3001 acctggtcga gcatgtccac gatcaggtcg tgggtcatcg gccgcggcgt gtcggcaccc
     3061 tggagcttca tcaggatcgc ggccgcttca gggtgcccga tccagatcgg caggtagcga
     3121 ttgtcgtcga tcgtcttcag caaaacgatc ggctgcttgc cgaccatgtc gaagctgacg
     3181 ccatagatct gaacctcacg catggatccc gattataggt aggacgagcc ctggtcacct
     3241 catcagccgt tccgccgtgt cggctcgaac ggaggtcctc ccatccgtca ttttgtgtga
     3301 gcgggaaggc cggttgagat tggagggcgg gcgcggaccc gccggactgc ggtcaagcct
     3361 atgggcgatc ctggattcga accagggacc tcatccttat cagagatgcg ctctaaccaa
     3421 ctgagctaat cgccctcgag gaaccgagga ttctaatggg ggtcgggccc gggtgtcgtg
     3481 aggtgaggag acaccgcgcc gggacgagcc cggagcctcg acttacttga gacgcctggc
     3541 gatctcagcc gcctgggcga ggtcctcgag ttcgatttcg accaccagcc ccttcccctt
     3601 cggtctgatt ttcaccgggc gatcaagttt ggtttccagc cgctccgttg cttcggcgac
     3661 ggcttcactc tcctcggccg acagaccctg cttgcggccg cccttcttcg gcgccgcagc
     3721 gagagcgcga gccccggcct cggtctgacg aaccgaccag ccttccttcc tggcctgttt
     3781 ggccagcttg cggcgatcgg cgtggtcggg aacctggagg atcgcgcgtc catggccttc
     3841 ggaaagttcg cccccttcga gcatctcgag cacgtcgtcg ggcaggtcga gcagccggat
     3901 caggttggag atctgggggc ggctgcggcc aacccgcttg ccgagttcct ccttgctgag
     3961 accgagatcc tcgaccaggg cggcgcaggc ctttgcctct tccaccggat tcaggttttc
     4021 ccggaccatg ttctcgatca gggcgacctc gagccggtcg gcttcatcgg aatcacggat
     4081 gaccaccggg accttttcga ccccggccat ctgtgcggcc cgccagcgtc gctcgccggc
     4141 gatgatctcg tactttccgc gcccagccgg ccgcacgatg agtggttgga ccactccact
     4201 ggcccggatc gattcggcga gggcctcgag agcctcctcg gaaaagttgg tccggggctg
     4261 ctccggattg cggtcgatcg actttaccgg cagctcgcgc atgtcaggcc cggtggcgct
     4321 ttccggcagg atcgccccga ggccccgccc cattccgcgc cgctcagcca cgtgccatca
     4381 cctcttcggc cagggcaaga taggcctcgg aaccccttga tgaaggggaa tagtcggtca
     4441 ccgggatgcc gtaactcggg gcctccgcga cgcgcacggt cctcggtatc accgtccgga
     4501 acacagtctc ggagaagtga tcgcgaagct cacgctcgac atcctgggca agtctggtcc
     4561 gctcatcatg catggtgacg aggacgccag ccagctccag ggtcgggttc atctcccgcc
     4621 gcac
//
LOCUS       DAOQKN010000186         4588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_10611_length_4588_cov_5.927421, whole genome shotgun sequence.
ACCESSION   DAOQKN010000186 DAOQKN010000000
VERSION     DAOQKN010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4588
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10611_length_4588_cov_5.927421"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1684
                     /gene="recN"
                     /locus_tag="PLJ64_10690"
     CDS             <1..1684
                     /gene="recN"
                     /locus_tag="PLJ64_10690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNC93993.1"
                     /translation="EAGLDISTANTLLIERAELRLGPGLNVLTGETGAGKTVLAHSLD
                     LLMGGKAKKGIVRSGAPEAWVEGVFDLPPEWHGDEELADLLDRIPAGSEEVVLGRRVS
                     ESGRTSAFVGGRSASAADLQLLATRLIAFYGQHEHRRLTIGSAQLAMVDRAAGLKQQE
                     RLNEYGSLWRRRRELARELDELLGRDMARERDLDLLRFELDEIEAASLIEGEKEVLTE
                     ERDRLRHAEGLREAAAGAGGRIRGGEDGEGAVGLLAGATDALAALKGVDADLDSLAGR
                     VESLALEMEDVGLELGRYLERVEADPERLAELEERLDLIDRLGRKHGGTIESVLAHGA
                     WCREEIERLEGGESKEAALRSELEAAKASLDQAAKGLGRARRKAAGELAAEVTADLED
                     LAMPGAVLSIELPPVADGPGPSGGESAEFMLAPNPGLPAQPLRDTASGGELSRVMLAL
                     AASGAGDRRTLVFDEIDAGIGGNTAAAVGERLRQVADGRQVIAITHLPQVASRAAAHF
                     TVEKSTGAGSVCATVTALDEDAVVTEITRMLGAGEGDEAAARHARELVMANR"
     gene            1755..2903
                     /gene="steA"
                     /locus_tag="PLJ64_10695"
     CDS             1755..2903
                     /gene="steA"
                     /locus_tag="PLJ64_10695"
                     /inference="COORDINATES: protein motif:HMM:NF040608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HNC93994.1"
                     /translation="MSEADAGGVPTSRGRIRLGRKTKDLVKTLGPADVAVIGHRNLDR
                     IAAEDLVASGVKVVLNNDFSSDGRYPNRGPLILVGAGVRLIDFPGIDLFETLVENEQV
                     VVEGDEVKRDGLTVARGVEQLGPDLEQQFERQQSEIHHALADFAENTVNHIQEESELL
                     SGAIELPDTRTDFRDRHVLIVVRGPNYKRDLRALRAYIRDVRPILIGVDGGADAIREA
                     GYRPDMILGDMDSVTDDTLKSGPEVVVHAYADGRAPGAERMKLLGVDHIALPFVGTSQ
                     DVAMLMAYEKGASLIVTVGAHFNLIEFLDKDRAGMSSTFLTRLRIGEVLVDAKGVSRL
                     YNPGLTVGPLMLFLGAFLVLVAIVVLSSPALADLFDLIWLKIKIWLGI"
     gene            2914..3828
                     /locus_tag="PLJ64_10700"
     CDS             2914..3828
                     /locus_tag="PLJ64_10700"
                     /inference="COORDINATES: protein motif:HMM:NF022824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HNC93995.1"
                     /translation="MGYSARYHAASLAAVFLALAIGILIGSQWGSDVLNNTREDLESS
                     LTSDLNDARDQIDELNQEQDWSNEFGSTVYPLLTANRLRERQVGLIGLGPLPSNVTDS
                     VESALEPAGAELVAVGAIRQPPSLDDLAAELQGTPYRQIASSDEVLVSYGRRVGRQLI
                     RGGRLLNLTRSDLMSQSSGQFDQLDGLIFYRAEPDEIDPEEVDTAEMLDRSIIDGAAT
                     TRARLVGIETTGTDPSTVGFLRDLNLTTVDNLDQPAGKVSLVYALNGAEGAFGVGDGA
                     TRIMPELLNPVAPGDGGQGQNGQGRAEP"
     gene            complement(3807..4118)
                     /locus_tag="PLJ64_10705"
     CDS             complement(3807..4118)
                     /locus_tag="PLJ64_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93996.1"
                     /translation="MSEAERVVKQTEKGDADQVDDPASQLRFEQVEAGPLVQNRQGSQ
                     RDEGSGNQYRAERKGKLLAPVVDPGQASPEEPADAGGDDQQADEDGDGETEPGYGSAL
                     P"
     gene            4230..>4588
                     /locus_tag="PLJ64_10710"
     CDS             4230..>4588
                     /locus_tag="PLJ64_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC93997.1"
                     /translation="MTGTGNEWPSYIADVALLILTTNLFNLLDLRGGRVEKVLFLMVI
                     GLCLGGWELFPIELTGVFLGPFAVGIFWTLRDRAMLGDTGSNLAGAIAGVCLLTGLDE
                     TGRLIALAAVILLTIYG"
BASE COUNT          771 a         1484 c         1587 g          746 t
ORIGIN      
        1 cgaagccggt ctcgatatct cgaccgccaa cacgctgctg atcgagcggg ccgaactcag
       61 gctcggtccc gggctgaacg tgctgaccgg cgagaccggc gccggcaaga ccgttttggc
      121 ccattccctg gacttgctca tgggcggcaa ggcgaagaag gggatcgtcc gcagcggcgc
      181 cccggaggcc tgggtcgagg gcgtcttcga cctgccgccg gaatggcacg gggatgagga
      241 actggccgac ctgcttgacc gcatcccggc cggcagcgaa gaagtggtgc tgggccggcg
      301 ggtttcggag tccggccgga cctcggcctt cgtcggcggc cgcagcgctt ccgcggccga
      361 cctgcagttg ctggcgacca ggttgatcgc cttctacggg cagcacgaac accggcgcct
      421 gaccatcggg tccgcgcagc tcgcgatggt tgaccgggcg gccggcctga aacagcagga
      481 gcgcctgaac gaatacgggt cgctctggcg aaggcgtcgt gaacttgccc gcgagctcga
      541 cgaactgctc ggccgggaca tggcccggga acgggacctc gacctgctcc gtttcgaact
      601 ggatgaaatc gaggccgcca gcctgatcga aggcgagaag gaagtgctga ccgaggaacg
      661 ggaccggctg cgccacgcgg aagggctgcg ggaagccgcg gcgggcgccg gtggccgaat
      721 tcgaggcggc gaggacggtg agggggcggt cggtctgctg gccggagcga ccgacgccct
      781 ggcggcactg aaaggtgtcg atgccgacct tgactccctg gccggaaggg tcgagagcct
      841 cgctctggag atggaggacg tcggcctcga actgggccgg tacctcgagc gggtggaggc
      901 cgatcccgaa cggctggccg agcttgagga gcgactcgac ctgatcgacc ggctggggcg
      961 aaagcacggc ggcacgatcg aatccgtcct cgcccatggc gcctggtgcc gcgaggaaat
     1021 cgaacgcctc gaaggaggcg aatcgaagga ggccgcactg cggtccgagc tggaagcggc
     1081 aaaagcaagc ctcgatcagg cagcgaaggg tctcggccgg gcccgcagga aggccgccgg
     1141 cgaactggcg gccgaggtta cggcagatct cgaggacctg gccatgcccg gggcggtgct
     1201 ttcgatcgaa ctgccgccgg tggccgacgg gcccggtcct tcgggcgggg aatcggccga
     1261 gttcatgctg gctccgaatc ccgggcttcc ggcccaaccg ctccgcgaca ccgcatccgg
     1321 aggcgaactt tccagggtca tgctggccct ggcggcgagc ggggcgggtg accggaggac
     1381 gctcgtattc gacgagatcg atgccggaat cggcggcaac acggcggcgg cggtcgggga
     1441 acggctgcga caggtggcgg atggccgtca ggtgatcgcg attacccacc tgccgcaggt
     1501 ggcaagccgg gccgcggctc atttcacggt cgaaaagtcg accggcgcgg gttcggtctg
     1561 tgcgaccgtg acggcgctcg acgaggatgc cgtcgtcacc gagatcacgc ggatgctcgg
     1621 agcgggcgaa ggcgacgaag ccgcggcccg acacgcacgc gaactggtga tggcgaaccg
     1681 gtaaccagca cttcccttac ccttgggggg tgaacgcgat cacgaaaagg ctgcgggggc
     1741 ggcccgcttc cgccatgtct gaagcggacg cgggcggggt tccgaccagt cgcggccgga
     1801 tccggcttgg gcgcaagacc aaggacctgg tcaaaacgct cggaccggct gacgtcgccg
     1861 tgatcggtca ccgcaatctc gaccggatcg cggccgaaga cctggttgct tccggagtaa
     1921 aggtcgtgct caacaacgac ttctcctccg acggtcgcta cccgaatcgc ggaccgctga
     1981 tcctggtcgg cgccggtgtc cgcctgatcg actttcccgg aatcgacctc ttcgagaccc
     2041 tggtcgagaa cgagcaggtg gtggtcgaag gtgacgaggt caagcgggac ggcctgaccg
     2101 tcgccagggg cgtcgagcaa ctcggaccgg atctcgaaca gcagttcgag cgtcagcaat
     2161 cggagatcca ccacgctctg gccgacttcg ccgagaacac ggtcaaccac atccaggagg
     2221 aaagcgaact gctttccggg gcgatcgaac tgcccgacac gcgcaccgac ttccgcgacc
     2281 ggcacgtgct gatcgtggtc cgcgggccca attacaaacg cgacctgcgg gcgcttcgcg
     2341 cctacatccg ggacgtgcga ccgatcctga tcggtgtcga cggtggcgcc gacgcgatca
     2401 gggaagccgg ctaccgcccc gacatgattc tcggggacat ggactcggtg accgatgaca
     2461 cgctgaagtc ggggcccgag gtggtcgtgc acgcctacgc cgacggtcgg gctcccgggg
     2521 cggaacggat gaagctactg ggggtcgacc acatcgccct gcccttcgtc gggaccagcc
     2581 aggacgtggc gatgctgatg gcttatgaaa agggcgcttc gctgatcgtc acggtcgggg
     2641 ctcacttcaa cctgatcgag ttcctcgaca aggaccgggc cggcatgtcc tcgaccttcc
     2701 tgacccggct caggatcggc gaggtactgg tcgatgcgaa gggtgtgagc cgcctttaca
     2761 accccggtct cacggtcggc ccgctgatgc tgttcctcgg ggccttcctg gtgctggtcg
     2821 cgatagtcgt gctgagctcg ccggccctgg ctgatctgtt cgatctgatc tggctgaaga
     2881 tcaagatctg gctgggcatc taggcttccc ggaatgggtt acagcgctcg ctaccacgcg
     2941 gcctcgcttg cggcggtctt tctcgccctc gcgatcggca ttctgatcgg ctcccaatgg
     3001 gggtccgacg tgctgaacaa cacccgcgag gacctcgaat cgagcctgac cagcgatctc
     3061 aacgacgccc gcgaccagat cgacgagctg aaccaggaac aggactggtc aaatgaattc
     3121 ggcagcaccg tctatccgct gctgaccgca aaccgcctgc gggaacggca ggtcggcctg
     3181 atcgggctcg gtccgctgcc ttcgaacgtg accgattcgg tcgaatccgc acttgagccg
     3241 gcaggggccg agctggtcgc ggtcggggcg atccgtcaac cgccttcact cgatgacctg
     3301 gctgcggaac tgcagggcac cccgtaccgc cagatcgctt ccagcgacga ggtcctcgtc
     3361 tcctatggcc gccgggtggg aaggcagctg atcaggggag gacgacttct gaacctgacc
     3421 cgcagcgacc tcatgtccca gtcgagcgga cagttcgacc agctcgacgg cctgatcttc
     3481 taccgggccg aacccgatga gatcgaccct gaagaagtgg acacggccga gatgcttgac
     3541 cggtcgatca tcgacggtgc cgccacgacc cgggcccggc tggtcgggat cgaaacgacg
     3601 ggcaccgacc cttcgaccgt tggcttcctt cgcgacctca acctgaccac ggtcgacaac
     3661 ctcgatcagc cggccggcaa ggtcagcctc gtttacgcgc tgaacggagc cgagggggcc
     3721 ttcggggtgg gggacggcgc gacccggatc atgcccgagc tgctgaaccc ggtcgctccc
     3781 ggtgacggcg gccagggcca gaacggtcag ggcagggccg agccgtaacc tggctcggtt
     3841 tccccgtcgc catcctcgtc ggcctgctgg tcgtccccgc cggcgtccgc gggctcctcg
     3901 gggctggcct gaccagggtc aactaccggg gcgagaagct tgcctttccg ctcggcgcgg
     3961 tactggttac cgcttccctc gtcgcgctgg ctcccctggc ggttctgaac gagcgggccg
     4021 gcctcgacct gctcgaaccg gagctgagac gctggatcat ctacctgatc ggcgtcgcct
     4081 ttctcggtct gcttgacgac tcgctcggcc tcggacacgc cgaaggcacc cctcggggct
     4141 ggcgcggcca cgcgtcggcc gtcctcaggg gggagctttc aaccggcgcg gtcaaggcga
     4201 tcggcgcact cgcgctggcc gcctatgtgg tgaccggaac cggaaacgag tggccttcct
     4261 acatcgccga cgtggcgctc ctcatcctga ccaccaacct cttcaacctg ctcgacctgc
     4321 gtggcggaag ggtcgaaaag gtgctttttc tcatggtgat cgggctttgt cttggcggtt
     4381 gggagctgtt cccgatcgaa ctgaccgggg tcttcctcgg accattcgcg gtcggaatct
     4441 tctggacgct tcgtgaccgg gcgatgctcg gggacaccgg ctcgaacctt gccggggcga
     4501 tcgccggggt ctgcctgctg accggactcg atgaaaccgg ccggctgatc gcgctcgcgg
     4561 cagtgattct tctcacgatt tacggaga
//
LOCUS       DAOQKN010000187         4309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_11692_length_4309_cov_6.574753, whole genome shotgun sequence.
ACCESSION   DAOQKN010000187 DAOQKN010000000
VERSION     DAOQKN010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11692_length_4309_cov_6.574753"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1167
                     /locus_tag="PLJ64_10715"
     CDS             <1..1167
                     /locus_tag="PLJ64_10715"
                     /inference="COORDINATES: protein motif:HMM:NF012946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNC93998.1"
                     /translation="TMYESAHLISSKTTTAYDEFPMREEVADFPSHWEMNSYFKDYAD
                     TFDLRRNFRFGTEVTAAEPEDPESESSVWKVTIRNEDGEEESGRYAGVIIANGILSEP
                     SIPTFEGEEEFEGEVLHTASYKRPDIFRDRKVLIVGCGNSGCDIAVDAVHYAKSIEMS
                     LRSGYWFFPKYIFGRPSDTLNEGKALPAFLKTRIDGVFIKFLTGDPARLGFPEPEGRI
                     YETHPVMNTQVLYYAGHGDIHIRKNIKRFTKTGVEFVDGSTGDYDMVMLATGYKLHYP
                     FIDPQYLNWKGNSPDLYLNIFTPKFRNIFVLGMVEATGLGWQGRYNQAHLVAEFIDAG
                     QRNPVRADEMWRRIQGPKPDLFAGYNYRQLDRLPFYVNKDAYRAQIHEHLEIFE"
     gene            1164..2147
                     /locus_tag="PLJ64_10720"
     CDS             1164..2147
                     /locus_tag="PLJ64_10720"
                     /inference="COORDINATES: protein motif:HMM:NF024981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HNC93999.1"
                     /translation="MSLAFLSSLPGLPFGAEDIDSVTLNTSDGMLIVLSVIIGVIMFG
                     IALEMKVDDFRHVMKLPKAMAVGVAAQFICLPAITYLLTVALNFRPSIAMGMILVACC
                     PPGNISNILVYRARGNVALSLSMTALSNFLAIFLMPLNIAFWGGMHPEASQVLKDVDV
                     SAIELLLDIVVLIGIPLTLGLLISSHRPGLAKKVQPIVKKFSLIFLILFIVAALAGNF
                     KVFVDHIALVAVAVFLHDTLALLLGYGIAAGFKVSEPNRRAITFEVGVRNAALGLGIA
                     ISVFDGLGGVAMVAAWWGVWDLIAGLTLSSWWARRPTGDIEESTLEAAESA"
     gene            2140..3114
                     /locus_tag="PLJ64_10725"
     CDS             2140..3114
                     /locus_tag="PLJ64_10725"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNC94000.1"
                     /translation="MPEAGGGMKRVFVTGGNGFLGHSVVRGLAEAKDQVELVVSGDLG
                     HPTDARRVEGVDYIVTDVTDAASVSGPIGEHGIDTVVHLAAIVNPGNLSTEQEYEVDV
                     TGSKHVFDACLAHDVKRVVVSSSGAAYGYHADNPAWLTEDDPVRGTETFPYSLHKRLV
                     EEQLAALRESNPELEQVVLRIGTILGETVDNQITDLFKAKRPLKIRGSESPFVFIWDQ
                     DVVGVMLQAVLGEKTGIFNVAGDGAMTIDEIAAELGKKPRTVPVGVLKAGLGVAHFFN
                     LTPHGAAQTDFLAYRPVLDNRRLKVEFGYTPKYTTREAFHAWRQSQGL"
     gene            3502..3575
                     /locus_tag="PLJ64_10730"
     tRNA            3502..3575
                     /locus_tag="PLJ64_10730"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3536..3538,aa:Pro,seq:tgg)
     gene            3596..3991
                     /locus_tag="PLJ64_10735"
     CDS             3596..3991
                     /locus_tag="PLJ64_10735"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNC94001.1"
                     /translation="MPRDSHPSATVHRRIEFSDVDSSGRYHYGTVIRLLEAAEVELLS
                     GLGLLDSIYTSMPRARLEVDYLSTLRFMDEVDVTVEVEDLGRTSVTIGFTVERGGEAC
                     ARGRLVTVFVDQQGTPAPWPAEARTAFGG"
BASE COUNT          754 a         1418 c         1346 g          791 t
ORIGIN      
        1 acgatgtatg agtcggccca cctgatctcc tcgaagacga ccaccgccta cgacgagttc
       61 ccgatgcgcg aagaggtcgc cgattttccc agccattggg aaatgaacag ttacttcaag
      121 gattacgcag acaccttcga cctgcgaagg aacttccgtt tcggcaccga ggtgaccgcg
      181 gccgagcccg aggatccgga gtcggaatcg tcggtctgga aggtgacgat ccgaaacgag
      241 gacggggagg aggagagcgg acgctacgcc ggggtgatca tcgccaacgg catcctctcc
      301 gagcccagca tcccgacctt cgagggcgag gaggagttcg agggcgaggt gctccacacc
      361 gcctcctaca agcgccccga catcttccgc gacaggaagg tgctgatcgt gggctgcggc
      421 aactcgggct gcgacatcgc cgttgacgcg gtccattacg cgaagtcgat cgagatgagt
      481 cttcgctccg gctactggtt cttccccaag tacatcttcg gccggccctc cgacaccctg
      541 aacgagggca aggccctgcc ggcctttctc aagacccgaa tcgacggggt cttcatcaag
      601 ttcctgaccg gtgacccggc ccggctcggc ttccccgagc cggaggggcg gatctacgag
      661 acccacccgg tgatgaacac ccaggtgctc tactacgccg gccacggtga catccacatc
      721 cgcaagaaca tcaagcgctt cacgaagacc ggagtcgaat tcgtggacgg ttcaaccggt
      781 gactacgaca tggtcatgct ggccaccggc tacaagctcc actacccgtt catcgacccg
      841 cagtacctga actggaaggg caacagtccc gacctctacc tgaacatctt caccccgaag
      901 ttccgcaaca tcttcgtgct cgggatggtc gaggcgaccg gtcttggctg gcagggtcgc
      961 tacaaccagg cccacctggt cgccgagttc attgacgccg gccagcggaa cccggtccgg
     1021 gccgacgaga tgtggcgtcg gatccagggc ccgaagccgg acctcttcgc cggctacaac
     1081 tatcgccagc tcgatcggct tccgttctat gtgaacaagg acgcctaccg ggcccagatc
     1141 cacgagcacc tggagatctt cgaatgagtc tggccttcct ttcctccctg ccgggcctgc
     1201 ctttcggggc ggaagacatc gacagcgtga ccctgaacac cagcgacggc atgctgatcg
     1261 tgctcagcgt gatcatcggg gtgatcatgt tcggcatcgc cctcgagatg aaggtcgatg
     1321 acttccgtca tgtgatgaag cttcccaagg cgatggcggt cggcgtggcg gcccagttca
     1381 tctgcctgcc ggcgatcacc tacctgctca cggtggccct caacttccgg ccctcgatcg
     1441 ccatggggat gatcctggtc gcctgttgtc cgccgggaaa catctcgaac atcctcgtgt
     1501 accgggccag aggaaacgtg gccctgtcgc tttcgatgac cgccctttcg aacttcctcg
     1561 cgatcttcct gatgccgctc aacatcgcct tctggggagg catgcatccg gaggcatccc
     1621 aggtcctcaa ggacgttgac gtcagcgcga tcgaactgct gctcgacatc gtcgtgctga
     1681 tcggaattcc gctgacgctc ggcctgctga tctccagcca ccgcccgggc ctggccaaga
     1741 aggtccagcc gatcgtcaag aagttcagtc tgatcttcct gatccttttc atcgtcgcgg
     1801 ccctggccgg caacttcaag gtcttcgtcg accacatcgc cctggtcgcg gtcgccgtct
     1861 tccttcacga cacgctcgcg ctgcttctcg ggtacgggat cgcggccggc ttcaaggttt
     1921 cagagccgaa ccgaagagcg atcacattcg aggtcggggt gcgcaatgcg gcgctcggtc
     1981 ttggcatcgc gatcagcgtc ttcgacggtc tcggaggcgt ggcgatggtg gccgcctggt
     2041 ggggggtctg ggacctgatc gccggcctga ccctctccag ctggtgggcg aggagaccga
     2101 ccggggacat agaagagagc acgctggagg cggccgaaag tgcctgaagc cggcggcggg
     2161 atgaagcgcg tattcgtcac cggcggcaac ggtttccttg gccattcggt ggtgcggggc
     2221 ctggctgagg cgaaggacca ggtcgagctc gtcgtctcgg gtgatctcgg ccacccgacc
     2281 gacgcgcggc gggtcgaagg ggtcgattac atcgtcaccg acgtgaccga tgccgcctcg
     2341 gtttccgggc cgatcggcga gcacgggatc gacacggtcg tccacctcgc ggcgatcgtc
     2401 aatcccggca acctctcgac cgaacaggaa tatgaggtcg atgtgaccgg gtcgaagcat
     2461 gtgtttgacg catgcctcgc tcacgatgtg aagcgtgtgg tggtctcttc cagcggcgcg
     2521 gcctacggct accacgccga caacccggcc tggctgaccg aggacgatcc ggtccgcggc
     2581 accgaaacct tcccctacag cctgcacaag cgtctggtcg aggagcagct ggcggcgctt
     2641 cgggaatcca atccggagct cgagcaagtg gtgctccgga tcggcacgat ccttggcgag
     2701 acggtggaca accagatcac cgacctcttc aaggcgaaac gaccgctgaa gatccgcggc
     2761 tccgagtcac cgttcgtctt catctgggac caggacgtgg tcggggtgat gttgcaggcg
     2821 gtgctgggcg agaagaccgg catcttcaac gtcgcgggtg acggcgccat gacgatcgac
     2881 gagatcgccg ccgagctcgg caagaagccc cgcacggttc cggtcggggt cctgaaggcc
     2941 ggtctcggcg tcgcccactt cttcaacctg accccgcacg gcgcggccca gaccgacttc
     3001 ctcgcctacc gcccggtcct cgacaaccgc cgcctgaagg ttgagttcgg ctacaccccg
     3061 aagtacacaa cccgcgaggc cttccacgcc tggcgccagt cccagggact ctgaacctcc
     3121 cacgccgccg gcccggcccc gccagccgcc attccctggg tctctccgag tccggcccgg
     3181 gccccgctag ccgccattcc cggggtctct ccaagtccgg cccgggcccc ggccccgccc
     3241 gttgagtccc agggtctctg gccggccccg gacccagacc gccaggttcc actttgcgcc
     3301 acatatcgca cccaactccg agctcgatcg ggagcggccg atcgagatcg gagttgggag
     3361 cgctatgtac caacttctcc cttctcgatt ccacgagcaa cggggtcctt caggtggggt
     3421 caagcgacac cgactgcgcg tcaggcttcg ggcatggatg gatcgcatcc gaggcgagtg
     3481 gaagtagaat cgtcaccgct tcggggtgta gcgaagcctg gtatcgcgtt cgctttgggg
     3541 gcgaaagatc cccggttcga atccgggcac cccgattggt cgaacacccg cgcccatgcc
     3601 tcgagattcc cacccctcag ccaccgttca tcgccggatc gagttttccg atgttgattc
     3661 ttcgggccgg taccactacg ggaccgtgat ccggttgctg gaggcggccg aggtcgagct
     3721 gctttccggg ctgggtttgc tcgattcgat ctacacctcg atgccgcggg cccggctgga
     3781 ggtcgactat ctctcgaccc tgcggttcat ggacgaggta gacgtgacgg tcgaggtcga
     3841 ggatctcggc cgcacctcgg tcaccatcgg attcacggtc gaacgcgggg gagaggcctg
     3901 cgcccgaggc cgcctggtca ccgttttcgt cgaccagcag ggcacaccgg ccccctggcc
     3961 cgccgaagcc cgcaccgcct tcggcggctg aaagccgcgg gggcgcaagc acccagggga
     4021 tcgatcactt cgaccggaca ttcgacgagg cgggcgtggc aagaacttgt ggcccgcttg
     4081 cgagcgtttg acccggccgc cccggtttct cgatgtttct gatcgttgtt cgatttcccg
     4141 ggcgatgccc gataggatcg agatgaatcc gccttcgggc aaaaacggga atcgggggaa
     4201 gttgagacgt ttaacgggat cgttggtggg gggacttgcg ttggtgtgcg ccttcgtgct
     4261 gccgtcgtcg gcaggggcga cgaccgtgac cttcaactac accggtgcc
//
LOCUS       DAOQKN010000188         4242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_12017_length_4242_cov_4.795558, whole genome shotgun sequence.
ACCESSION   DAOQKN010000188 DAOQKN010000000
VERSION     DAOQKN010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12017_length_4242_cov_4.795558"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..2068)
                     /locus_tag="PLJ64_10740"
     CDS             complement(47..2068)
                     /locus_tag="PLJ64_10740"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HNC94002.1"
                     /translation="MSEETKDTKPLLPGIASPADLKGLSDEQLQRVADETRSYIIDVI
                     GEIGGHFGANLGTCEIAVALHSLLESPRDKILWDVGHQAYPHKVLTGRRDRLSTIRQY
                     EGLAPFCSIPESEHDIMGAGHASTSISYGVGLKEAMRKGIGEDGKVVAVIGDGALTGG
                     VAYEAMHAAGGYQTPMVILLNDNGMSISPNVGALSSYFQRARLKPGLFHAREEVEDRL
                     TRLPLGLGKRIERLGPELKSAIKAYWAPGLLFEELDLAYMGVIDGHDVGAMRRALTEA
                     FAADRPVVVHVHTVKGRGFAPAEQGGQAGQEKWHAAKPGSIVDGAPADSKPKLVPVQE
                     SDAPASIAPEILEMPEPKAAPPQYTQVFADSLVSEAARDPRVIGITAAMAGGAGLQKL
                     ADERPDQYYDVGIAEQNAVLMASGIALQGGKPVCGIYSTFLQRAYDQIVHDVCLQELD
                     VTFAMDRAGLVGDDGPTHHGAFDIAYFRSLPNVVAMAPRDEAMLVHMLHTAIEHDGPA
                     ALRYPRGAAEGVEIPGGPELIPIGTGETLRNGERVALVGYGSGVGVALGAADLLDEEG
                     IRPTVADARFVKPLDTELMERLAVSHDLIVTIEEGVLQGGFGSAVIEHLEDNFANPGE
                     RARVLRVGLPDRFVTHGKPELLHREVGFTAEAVAEKVLAAVESGVAPRH"
     gene            complement(2065..2970)
                     /locus_tag="PLJ64_10745"
     CDS             complement(2065..2970)
                     /locus_tag="PLJ64_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNC94003.1"
                     /translation="MSIEGRKPLLSYPEELRELVDSHLETIHFSRGDGVDGAETTGLD
                     EAMRYSLLAGGKRIRPVLCLATARAIGQPTDDFLGAAAALEMIHTYSLVHDDLPAMDD
                     DDLRRGKPTSHVKYGEAVAILAGDGLFAEAVGELARSPATPERVLEAVSLLSAATGVE
                     GMVGGQYIDVSAQALDADGLRHLHSLKTGRLIRASIEVTLALGGVTEASREPFRRFAT
                     QIGLLFQIVDDILDVTGSDEELGKPQGSDERHGKVTYVSLYGLDRARSLAAETHEGAL
                     AALAGAGGETEDLKRIADYIYSREQ"
     gene            complement(2980..3153)
                     /locus_tag="PLJ64_10750"
     CDS             complement(2980..3153)
                     /locus_tag="PLJ64_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94004.1"
                     /translation="MSRLEEIRDRLDEITLALRDESVSDTDAAGLADEAAKLTAEAAS
                     EAAAAVERADRQA"
     gene            complement(3150..3647)
                     /gene="nusB"
                     /locus_tag="PLJ64_10755"
     CDS             complement(3150..3647)
                     /gene="nusB"
                     /locus_tag="PLJ64_10755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNC94005.1"
                     /translation="MRRSNQRRDAVFACYQQEVTDRPLSVLLAEAKPFTVELAEGVEA
                     HREDLDEVISEYAQGWDLSRIAPLEKNIMRVALYEMNFRDDVPFEVAIDEAVETAKKF
                     CGAEAPGFVNGILGAAARDMKADTEATNAGIETADTEATNAGTEEAGAEAVNTNTEAA
                     NGEAA"
     gene            3891..>4242
                     /locus_tag="PLJ64_10760"
     CDS             3891..>4242
                     /locus_tag="PLJ64_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HNC94006.1"
                     /translation="MTHLEVTNLISSLAARQDGVVNRRQLLAAGVAASQVDSRVRNRT
                     LKKLYAGVYAVGHDRLSPLGRQRAAVMAGGPNAVLSHRAAAGLHGLLPPNGILEVIRS
                     SSPDPHQPPPAHASR"
BASE COUNT          633 a         1455 c         1386 g          768 t
ORIGIN      
        1 agggtcgttt cccgccacga ctgagacggg cccgggaaac cggttttcag tgacgcggag
       61 cgactccgga ttcgaccgcg gcgagcacct tctcggccac cgcctccgcg gtgaagccga
      121 cttcccggtg gagcagttcg ggcttgccgt gggtcacgaa acggtccggc aagccgaccc
      181 gcagcacccg cgcccgctcg cccggattgg cgaagttgtc ttccagatgc tcgatcacgg
      241 ccgagccgaa accgccctgg agaacccctt cctcgatcgt cacgatcagg tcgtggctga
      301 ccgccagacg ctccatgagt tcggtgtcga gcggcttgac gaagcgggcg tcggccacgg
      361 tcggccggat cccctcttcg tccagaaggt ccgccgctcc gagcgccact ccgaccccgc
      421 tgccgtagcc gaccagggcg acccgctccc cgtttcggag ggtttcgccg gttccgatcg
      481 ggatcagttc cgggccgccc gggatttcga ctccttcggc ggccccgcgc ggatagcgca
      541 gcgcggccgg cccgtcgtgt tcgatcgccg tgtgaagcat gtggaccagc atcgcctcgt
      601 cgcgcggggc catcgccacc acgttcggga gcgagcggaa gtaggcgatg tcgaaggcac
      661 catggtgggt cggcccgtca tccccgacca gcccggcccg gtccatcgcg aaggtcacgt
      721 cgagctcctg caggcagacg tcgtggacga tctggtcgta ggcccgctgc aggaaggttg
      781 agtagatgcc gcagaccggc ttgccgccct ggagcgcgat gccgctggcc atcagaaccg
      841 cgttctgctc ggcgattccg acgtcgtagt actggtcggg ccgttcgtct gcgagcttct
      901 ggaggccggc cccgccagcc atcgcggcgg tgattccgat cacccgcggg tcccgtgccg
      961 cctcgctgac caaggagtcg gcgaagacct gcgtgtactg cggcggagcc gccttcggct
     1021 cgggcatctc gaggatctcg ggagcaatcg aggccggggc atcggattcc tgcaccggga
     1081 ccagcttcgg cttcgagtcg gccggagccc cgtcgacgat cgagccgggc ttggccgcat
     1141 gccatttctc ctggccggcc tggccgccct gctcggccgg ggcgaaaccg cggcccttga
     1201 ccgtgtggac gtggacgacc accggccggt ccgcggcgaa agcttcggtc agagcccggc
     1261 gcatcgcccc cacgtcgtgg ccgtcgatca cacccatgta ggcgagatcg agttcttcga
     1321 agagcaggcc gggggcccag taggccttga tcgccgactt gagttccggc ccgagccttt
     1381 cgatccgctt gccgaggccg agcggcagcc tggtcaggcg atcctcgacc tcttcgcggg
     1441 catggaagag cccgggcttc agccgggccc tctggaagta gctggaaagc gccccgacgt
     1501 ttggcgagat cgacatgcca ttgtcgttga gcagaatcac catcggggtc tggtagccgc
     1561 ccgcagcatg catcgcctcg taggcgacgc cgccggtcag ggcgccgtca ccgatcaccg
     1621 cgacgacctt gccgtcctcg ccgatcccct ttcgcatcgc ttccttgagg ccgaccccgt
     1681 aggagatcga ggtcgaagcg tgaccggcac ccatgatgtc gtgctcggat tccggaatcg
     1741 agcagaacgg ggcgaggcct tcgtactggc ggatcgtcga gagccggtcg cgccggccgg
     1801 tcagcacctt gtgcggatag gcctgatggc cgacgtccca gaggatcttg tccctgggcg
     1861 attcgagcag gctgtgcagg gcgaccgcaa tctcacaggt tcccaggttg gccccgaagt
     1921 gaccgccgat ttcaccgatc acatcgatga tgtaggagcg ggtctcatcg gcgacccgct
     1981 ggagctgctc gtcgctgaga cccttgaggt cggccggaga ggcgatcccg ggaagcagag
     2041 gcttcgtgtc tttggtctct tcgctcattg ttctctcgaa taaatgtagt cggcgatgcg
     2101 tttcaggtct tcggtttcgc cgccggcacc ggccagcgcg gccagggccc cctcgtgggt
     2161 ctccgcggcc agcgaccgtg cccggtccaa tccatacagg ctgacataag tcacctttcc
     2221 gtgacgctcg tcgctgccct ggggcttccc gagttcttcg tcgcttccgg tcacgtcgag
     2281 gatgtcgtcg acgatctgga agagcaggcc gatctgggtg gcgaagcgcc ggaacggctc
     2341 acggctcgct tccgtgaccc cgcccagcgc gagcgtcact tcgatgctgg cccggatgag
     2401 ccggccggtc ttcagcgaat ggaggtgcct gagcccgtcc gcgtccaggg cctgggccga
     2461 gacgtcgatg tactggccac cgaccattcc ttccaccccg gtcgcggcgc tgagcaggct
     2521 gaccgcttca agaacccgtt ccggggttgc cggagagcgg gccagttcgc cgaccgcttc
     2581 ggcgaagagc ccatcgcccg ccaggatcgc gaccgcctcg ccgtatttca catgtgaggt
     2641 cggcttgccg cgacgcaggt catcgtcgtc catggccggc aggtcgtcgt ggaccagcga
     2701 ataggtgtgg atcatctcca gggcggccgc ggccccgagg aaatcgtcgg tcggctggcc
     2761 gatcgccctg gcggtagcca ggcagagcac cggcctgatg cgcttgcctc cggccaggag
     2821 cgagtaacgc atggcctcgt cgagccccgt cgtctcggcc ccgtcgacgc catcgccacg
     2881 gctgaagtga atcgtttcga ggtgactgtc gaccagctca cgcagctctt ccgggtagct
     2941 caagagcggc ttccgccctt caatggacat tccacgggct caggcctgcc ggtcggcgcg
     3001 ctcgaccgct gccgcggctt cactcgcagc ttcggccgtg agcttcgcgg cttcgtcggc
     3061 cagacccgcc gcgtcggtgt cggaaacgct ctcgtcacgc agcgccagag tgatctcgtc
     3121 gagccggtcg cgaatttcct cgagtcgact cacgccgcct ccccattggc tgcttcggtg
     3181 ttggtgttga ccgcttcggc gccggcttct tcggtgccgg cgttcgtcgc ttcggtgtcg
     3241 gctgtttcga tgccggcgtt cgtcgcttcg gtgtcggcct tcatgtcccg ggcagcggcc
     3301 ccgaggatcc cgttgacgaa gccgggagcc tcggctccgc agaatttctt tgcggtctcg
     3361 accgcttcgt cgattgcgac ttcgaagggc acatcgtccc ggaagttcat ctcgtagagg
     3421 gcgacccgca tgatgttctt ctcgagcggc gcgatccggc tcaggtccca accctgggcg
     3481 tattcggaga tgacttcatc gagatcctcc cgatgagctt caacgccctc ggccagctcg
     3541 accgtgaacg gtttcgcttc ggcgagtagg acggagaggg gccgatcggt cacttcctgc
     3601 tggtaacagg caaaaaccgc atcccgacgc tgattggagc gacgcatcag ccaagaatat
     3661 tggttcgaac acccaacctc ccggtgggtc ggatttccgt cctccatgcg gactttccga
     3721 cccgcctcca ggcccgcacc ctcccccgtc ccgccggtgg gtcggattgt cggtcctgac
     3781 gctggaaatc cgacccaccg gcgagaggaa ccgcgagttt cccggggcca ggttgcaaat
     3841 cgttgcacct gtcgagccac gtgtgagcat ttccccggca catttggggg atgacgcatc
     3901 tggaggtgac caacctgatc tcttcgctcg ctgcccggca ggacggagtc gtcaaccgcc
     3961 ggcagctcct tgcggccggg gtggcggcca gccaggtcga ttccagagtc aggaaccgga
     4021 ccctgaagaa gctctacgcc ggcgtttacg cagtcggcca cgaccggctc tcgcccctgg
     4081 gcaggcagcg ggccgccgtg atggccggtg gtccgaatgc ggtgctttcg caccgtgcgg
     4141 cggccggcct tcacggcctg ctgccgccga acgggatcct cgaagtgatc cgcagctcaa
     4201 gccccgaccc tcaccagccg ccgcccgcgc acgcaagccg gg
//
LOCUS       DAOQKN010000189         4204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_12227_length_4204_cov_6.406604, whole genome shotgun sequence.
ACCESSION   DAOQKN010000189 DAOQKN010000000
VERSION     DAOQKN010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4204
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12227_length_4204_cov_6.406604"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="PLJ64_10765"
     CDS             complement(<1..305)
                     /locus_tag="PLJ64_10765"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNC94007.1"
                     /translation="MASFAPEEFTVEAGGRKLAGESQGEGPPILLCHGLSATRRYVVH
                     GSRHLPRQGYRLITYDARGHGESDPAVRYDYEGLGGDLDLVIEANPAENGMVLGGHS"
     gene            complement(308..958)
                     /locus_tag="PLJ64_10770"
     CDS             complement(308..958)
                     /locus_tag="PLJ64_10770"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNC94008.1"
                     /translation="MSERGKLVTLGVIGAVLAVVVLVVLVGRGGDDDDSSIATPVTKD
                     VKLSRPEPGSVVQPGEKASVKMETTEGDFTISLDTENWPVTANSFAYLTEEKFYDGLG
                     FHRIVPDFVIQGGDPTGTGSGGPGYSVVETPPKSTTYPVGTVAMAKTGAEAPGTSGSQ
                     FFIVTGAQGSSLTPDYSIAGTVSEGLEVVKRIGELGSASEQPTKQVVIEKATLEKG"
     gene            999..1652
                     /locus_tag="PLJ64_10775"
     CDS             999..1652
                     /locus_tag="PLJ64_10775"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC94009.1"
                     /translation="MPEYRSAADRKTILVGKTICRLAAVAPWAWPLIGKSVGRFFDRA
                     AAGWDERTAAGSAGHLATLAAGLDSLEVKPERVLDLGCGTGAGTLFLAREFPRAGIRG
                     VDLSESMISQAQAKIGLDPEGRVSFRVGDASKLPFGEDSFDLVTQVNMPVFFGEIARV
                     LRPGGGLIIATSLGEQTPFSTPERLIRRKLASHGFGELNSGAAGEGTWVSAKLEGSG"
     gene            1655..2542
                     /locus_tag="PLJ64_10780"
     CDS             1655..2542
                     /locus_tag="PLJ64_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HNC94010.1"
                     /translation="MKQALTLIMNPSSGGGRSKQLLPDVERRLDALRIPFRVERTRSL
                     EHGVDLAFEAVDNGEIPVVMSGDGLIGAVGGALAGVETKVGLIPAGRGNDLARGLGIP
                     TDPVQAVDCLAAGHTRAIDVGDANGKRFLGIASVGFDSDANRIANEARLLKGQLVYVY
                     AAVRALLAWKPARFSLVENGVQSRLTGYNVVVANNGFYGGGMNVAPDARIDDGQLDVI
                     TVGEAGKIRFLLNFPKVFKGTHVEEVESVSFHRTSVIEIGASRPFTIYADGDPLTELP
                     ATIRVIPSALQVIVPEPAS"
     gene            2539..3891
                     /locus_tag="PLJ64_10785"
     CDS             2539..3891
                     /locus_tag="PLJ64_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNC94011.1"
                     /translation="MIKAKASVARMVGRLSRATGKGGGTTLPGRVLLKLDANAIDKLG
                     ARLTDGTTLISATNGKTTTASMLATVLAGAGRHPVHNRAGSNMTWGVATALTEQRGCE
                     GLFEVDEAWLPEVADRLGPRAILLGNLFRDQLDRYGETEALADAWAGLAARRAGRTSF
                     ILNSDDPLIADIGNSSGPDTIYFGIEDEAQALTELQHAFDAKLCRNCGHELTYDRAFV
                     GHLGHYHCESCGMRRPRPEISATGITLKGMSGLTATINSPEGSVELDLPLPGLYNLYN
                     ALGAIATARQIGVPVSESVRSLAGMKAVFGRVERIRIGEVDLSILLVKNPAGANEVIR
                     TLSLEPAPIDLWIALNDRIADGRDVSWIWDADFEMLADRVGRVTCTGTRASEMALRLK
                     YAGWPTDRTEVIEDPEAAIDRALAQSGEQLFALPTYTALLDLRKLLADRGDTEKYWEG
                     "
     gene            3888..>4204
                     /locus_tag="PLJ64_10790"
     CDS             3888..>4204
                     /locus_tag="PLJ64_10790"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC94012.1"
                     /translation="MILSDENSPEAAIWQLVESCGYTADLPVWSGLAEGASSVLDLGC
                     GIGRVARHLAREGLPVTGVDLDPLLVDDLKRLSAPDPVHALAGDVTGLGGLDLGRERF
                     EVIL"
BASE COUNT          693 a         1444 c         1369 g          698 t
ORIGIN      
        1 atcgaatggc ctccgaggac catcccgttc tccgccgggt tcgcctcgat caccaggtcg
       61 aggtctccgc ccaggccctc gtagtcgtag cggacggcgg gatcgctttc accgtggccg
      121 cgggcgtcgt aggtgatcag ccggtaaccc tgccgcggca ggtgcctgga cccgtggacc
      181 acgtagcgtc gggtagccga aagcccgtgg caaagcagga tcggcggacc ttcgccctgg
      241 ctttcgccgg ccagcttcct gcccccggcc tcgaccgtga actcctccgg cgcgaaggag
      301 gccaagatca gcccttctcg agcgtcgcct tttcgatgac gacctgcttg gtcggctgct
      361 cgctggccga gcccagttcc ccgatccgct tgacaacctc gaggccttcg gacaccgtcc
      421 cggcgatcga gtagtccggt gtaaggcttg atccctgagc tccggtcacg atgaagaact
      481 ggctgcccga ggttccgggc gcttcggctc cggtcttggc cattgccacc gtgccaaccg
      541 ggtaggtggt gctcttcggc ggagtttcga ccacgctgta accggggccg ccggatccgg
      601 tgccggtcgg gtccccgccc tggatcacga agtcggggac gatccggtgg aagccaagcc
      661 cgtcgtagaa cttctcctcg gtcagatagg cgaagctgtt cgcggtgacc ggccagttct
      721 ccgtgtcgag gctgatcgtg aagtcaccct cggtcgtctc catcttcacg ctggccttct
      781 cgcccggctg gaccactgag cccggttcgg gccgggacag cttgacgtcc ttggttacgg
      841 gggtggcaat gctcgaatca tcgtcgtcac caccgcgccc gaccagcacc accaggacca
      901 cgacggccag gacagcgccg atcacgccaa gggtgaccag ctttccgcgt tcactcatcg
      961 gcgccagtct atggacttgc ctttgtaatg atcaggaggt gcccgagtac cgaagcgcag
     1021 ccgaccgcaa gacgatcctg gtcggcaaga ccatctgtcg cctggcagca gttgctccct
     1081 gggcctggcc gctgatcgga aagtcggtcg gtcgtttctt cgaccgcgcc gctgccggct
     1141 gggatgaacg cactgcggcc ggttcggccg ggcatctggc cactctcgcc gccgggctcg
     1201 actcactcga ggtgaaaccg gaacgggtgc ttgatctcgg ctgcggaacc ggcgcgggga
     1261 cgctctttct cgcccgcgag tttccccggg ccggaattcg cggtgtcgac ctttccgagt
     1321 cgatgatcag ccaggcccag gccaagatcg ggctcgaccc ggagggcagg gtctccttcc
     1381 gggtgggcga cgcttcgaaa ctgcccttcg gcgaggattc gttcgacctg gtgacccagg
     1441 tgaacatgcc ggtcttcttc ggcgagattg cccgggtcct gcgccccggc ggcggcctga
     1501 tcatcgccac cagccttggc gaacagaccc cgttcagcac cccggagcgg ctgattcgca
     1561 ggaaactggc gtcccacggc ttcggcgagc tgaactccgg cgcggcgggt gaaggaacat
     1621 gggtctcggc aaaactcgaa ggatccggct gaacttgaaa caggcgctga cattgatcat
     1681 gaacccgagc tccggcggcg gacgctcgaa acagctcctg ccggacgtgg aacggcggct
     1741 tgatgcgctg aggatcccct tccgggtcga gcgcacccgt tcgcttgaac acggggtcga
     1801 tctcgccttc gaggcggtcg acaatggcga gatcccggtg gtgatgagtg gcgacgggct
     1861 gatcggcgcg gtcgggggtg ccctggccgg ggtcgaaacg aaggtcgggc tgattccggc
     1921 cggtcgcggc aacgacctgg cccgcgggct cggcatcccc acagatccgg ttcaggccgt
     1981 cgactgcctg gccgccggcc acacccgcgc gatcgacgtc ggcgatgcca acggcaagcg
     2041 cttcctcggc atcgcctcgg tcgggttcga ctccgatgcg aaccggatcg ccaacgaggc
     2101 aaggctcctg aagggccagc tcgtctatgt ctatgcggcg gtaagggcgc tgctggcctg
     2161 gaagccggcc cgcttcagcc tggtcgagaa cggcgtccag agccgcctga ccggctacaa
     2221 cgtggtcgtc gccaacaacg gcttttacgg cggcggcatg aacgtggccc ctgacgcccg
     2281 gatcgacgac ggccagctcg atgtgatcac ggtcggcgaa gccggcaaga tccgcttcct
     2341 gctcaatttc ccgaaggtgt tcaagggaac ccatgtcgaa gaggtcgagt cggtctcgtt
     2401 ccaccgcacc agcgtgatcg agatcggtgc cagccgcccg ttcacgatct acgcggacgg
     2461 tgatccgctg accgaacttc cggcgacgat ccgggtcatc ccctcggcgc ttcaggtgat
     2521 cgtgccggag ccggcctctt gatcaaggca aaagccagcg tcgcccgaat ggtcggccgg
     2581 ctgagccgcg ccacgggcaa gggcggcggc accaccctgc ccggccgggt cctgctgaag
     2641 ctggacgcca acgcgatcga caagctgggc gcccgcctga ccgacggcac gaccctgatc
     2701 agcgcgacca acggcaagac gaccaccgcc tcgatgctgg ccacggtcct tgccggcgcc
     2761 ggccggcatc cggtccacaa ccgggccggc tcgaacatga cctggggtgt ggccaccgcc
     2821 ctgaccgagc agcgcggctg cgaaggactc ttcgaggttg acgaggcctg gctaccggag
     2881 gtcgccgacc gtctcggacc ccgggcgatc ctgctcggca acctgttccg cgatcaactc
     2941 gaccgctacg gggagaccga ggcgctcgcc gacgcctggg ccgggctggc cgcccgacgg
     3001 gccggccgga cctcgttcat cctcaactcc gacgacccgt tgatcgccga catcggcaac
     3061 tcaagcggcc cggacacgat ctatttcggg atcgaggatg aagcccaggc cctgaccgag
     3121 ctccagcacg cctttgacgc caagctctgc cgcaactgtg gccacgagct gacctatgac
     3181 cgggccttcg tcggccatct cggccactac cactgcgaaa gctgcggcat gaggcggccc
     3241 cggccggaaa tctcggcgac cggcatcacc ctgaagggca tgtcgggcct caccgccacg
     3301 atcaattccc ccgaaggcag cgtcgagctc gatctgcccc tccccggcct ctacaacctc
     3361 tataacgcgc tcggggcaat cgccacggcc cgccagatcg gagtgccggt ctccgaatcg
     3421 gtccggagcc tggccggcat gaaagcggtc ttcggccggg tcgagcggat caggatcggg
     3481 gaagtcgacc tgtcgattct gctggtcaag aacccggccg gcgccaacga ggtgatccgg
     3541 accctctcac tggaacccgc gccgatcgat ctctggattg cgctgaacga ccggatcgcc
     3601 gacggccgcg acgtctcctg gatctgggac gccgacttcg agatgctcgc cgaccgggta
     3661 ggcagggtca catgcaccgg aaccagggct tcggaaatgg cgctccggct caagtacgcc
     3721 ggctggccca ccgaccgcac cgaggtcatt gaagatcccg aagccgcgat cgaccgggcc
     3781 ctcgcgcaaa gcggagagca gctcttcgcc ctgccgacct atacggcgct gctcgatctg
     3841 cgaaaactgc tggctgatcg cggcgacacc gagaagtact gggaaggttg atcctcagcg
     3901 acgagaactc acccgaagct gcgatctggc agctggtcga gtcctgcgga tacacggccg
     3961 accttccggt ctggagcggg cttgcggaag gtgcatcatc cgtccttgac ctcggctgcg
     4021 gcatcggcag ggtcgcccgc cacctcgccc gggaagggct gccggtgacc ggggtcgatc
     4081 tcgatccgct gctggtcgac gacctcaaac gactctccgc cccggacccg gtccacgcac
     4141 tggccgggga tgtgaccggg ctgggcgggc tggacctcgg ccgggaacgc ttcgaggtca
     4201 tcct
//
LOCUS       DAOQKN010000190         4204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_12228_length_4204_cov_5.972282, whole genome shotgun sequence.
ACCESSION   DAOQKN010000190 DAOQKN010000000
VERSION     DAOQKN010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4204
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12228_length_4204_cov_5.972282"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..1550)
                     /locus_tag="PLJ64_10795"
     CDS             complement(3..1550)
                     /locus_tag="PLJ64_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain repeat protein"
                     /protein_id="HNC94013.1"
                     /translation="MKKFLVLAAALLALAFTAQTAQAEPEHELVFGEITVEVHGGGTV
                     TGTGINCGADCYDDDSWYDNEIPPTNRLTATAGRGWAFGGWEGCTSVNGQSNKCDAGY
                     QEFGGTPVIASFYDVMAPSVFLQFPALNTVANDTLHAAVESGDNDRVEKVEYLIDGQV
                     AKTVDSGGIDVYIDVTGVSEGQHEIQARAYDPTGNYGITAARTFTVDHSGPDIALNAP
                     LVATNADSPAFSFSTTATDIKNTWCAIVPKGDDVNYSACSPNEPFVGPGLAEGEWIFT
                     VWATDNVGNLTQITHDFVVDRTAPVADFTSGPADGATVKPGEVTYGWSATDGLAVAQH
                     CSWDGAEAVGCEGTASKNLAAGSHNFQVTIVDQAGNETTLSRSVTVKKATGPDPDPDP
                     DPDPDPDTTDRTAPVVKLAAPKQTLPGVRKALRLKVSCDEACSGKVTVTGKGVKFAGR
                     VYLTRAGVAKLKLRPTVKARRVLARLATRKPRSTGARSLGLTARASLKDAAGNTGRAS
                     LRFKVTA"
     gene            1733..2866
                     /gene="pdhA"
                     /locus_tag="PLJ64_10800"
     CDS             1733..2866
                     /gene="pdhA"
                     /locus_tag="PLJ64_10800"
                     /inference="COORDINATES: protein motif:HMM:TIGR03182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HNC94014.1"
                     /translation="MAELPDRQESLELLGRMCLIRRFEEEAGRQYQRAKAGGFLHLAI
                     GEEATIVGTTSVMREDDYLIGTYRTHGHALARGTPANEVMAELFGRQTGTSGGRGGSM
                     HIFDAGRRFMGGYGIVGGNLPLAAGLALASQYRDEDTVTVCMFGDGASNTGNFGETMN
                     LAALWKLPVLFLVENNLYGMGTSLERHSAVTDLSHKAAGYGIEGERIDGMNVLEVRER
                     VGEHLETVRSEGRPSLVEAFTYRYRGHSAADPEVYREKSEVEEWREKDPIENFIRLCC
                     DQGVLSDDDVEAARREADETVEAAVKFADESPEPALESLYEELYVVSETMGWYAVDER
                     SPEPHRGELGEDAPELARDLAESGAAHADGEDPEQQSRPGVRD"
     gene            2866..3891
                     /locus_tag="PLJ64_10805"
     CDS             2866..3891
                     /locus_tag="PLJ64_10805"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HNC94015.1"
                     /translation="MAELRMREALRDAMAEEMRRDESVFVMGEDVGVFQGAFKVTEGL
                     LDEFGEKRIRDTPISENTIVGVGVGAAMAGLRPVVELMTVNFSLLALDQIVNHAAAIP
                     YMFNGNVRVPMVIRMPGGGGHQLGPTHSHSFEALYLQVPGLLVACPSTPADAKALLKA
                     SIRDENPVIFIEHESLYGIKGEVPEGEDEILRFGEAAIRREGSDVTIVGILKMAHVAE
                     RAAAELEEEHGISAEVIDPRTLRPLDLDTILASVKKTNRAVIVEEGWPHGGVGANLST
                     LITEQAFDYLDAPVQRVTGADVHMPYSRRMEQAAIPHEENVIEAVLATTEGAFPPAGG
                     NSGEGDS"
     gene            3891..>4204
                     /locus_tag="PLJ64_10810"
     CDS             3891..>4204
                     /locus_tag="PLJ64_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl domain-containing protein"
                     /protein_id="HNC94016.1"
                     /translation="MAEIVMPRLTDSMEEGVILAWLKADGDEISVGDELVEIETDKAS
                     MVYESDLAGTLEILVPEGETRPIGEPIANVGDGSSSRGGGAVAVGDPVGRTSPGVPPG
                     PPT"
BASE COUNT          711 a         1325 c         1465 g          703 t
ORIGIN      
        1 cttcaggcag tgaccttgaa ccggagtgag gccctgccgg tgttgccggc ggcgtccttc
       61 aggctggccc gtgcggtcag ccccagggac cgggctccgg tgctccgcgg tttgcgggtg
      121 gcgagccggg ccaggacacg gcgggccttc acggtcgggc gcagcttgag cttcgccacc
      181 ccggcccggg tcaggtagac gcggccggcg aacttcacgc ccttgccggt gaccgtcacc
      241 ttgcccgagc aggcctcatc gcagctgacc ttgagacgca gcgccttgcg gacgccgggc
      301 agcgtctgct tcggcgccgc cagcttgacg accggtgccg tccggtccgt ggtgtccgga
      361 tccgggtccg ggtcggggtc gggatccggg tcgggaccgg tcgccttctt cacggtgacc
      421 gaacgcgaga gcgtcgtctc gtttccggcc tgatcgacga tcgtgacctg gaagttgtgc
      481 gaaccggcgg cgaggttctt cgacgcggtg ccttcacaac caacagcttc ggcgccgtcc
      541 cacgagcagt gctgggcaac ggccaggccg tcggtcgccg accagccgta agtgacctcg
      601 cccggcttga cggtcgcgcc atcggcgggg cccgaagtga aatcggcgac cggcgcggtg
      661 cggtcgacca cgaagtcgtg ggtgatctgg gtcaggttgc cgacgttgtc agtcgcccac
      721 acggtgaaga tccactcgcc ctcggcgaga cccggtccga caaacggttc gttcggactg
      781 caggcgctgt agttgacgtc gtcgcccttc gggacgatcg cgcaccaggt gttcttgata
      841 tcggtggcag tcgtgctgaa cgagaaggcc ggcgagtcgg cgttggtcgc gaccagcgga
      901 gcgttcaagg cgatgtccgg accgctgtga tcgacggtga aggtgcgggc ggcggtgatt
      961 ccgtagttgc cggtcgggtc gtaggcgcgg gcctggatct cgtgttggcc ttcggaaact
     1021 ccggtgacgt cgatgtacac gtcgatgccg ccgctgtcca cggtcttggc aacctggccg
     1081 tcgatcaggt actcgacctt ttcgacccgg tcgttgtcac cggattcaac cgcggcgtgg
     1141 agcgtgtcgt tggcgaccgt gttcagggcc gggaactgca ggaacacgct cggtgccatc
     1201 acgtcataga agctggcgat gaccggggtg ccgccgaact cctgatagcc ggcgtcgcac
     1261 ttgttcgact ggccgttgac cgaggtgcaa ccctcccagc cgccgaaggc ccagccgcgg
     1321 ccggccgtcg cggtgagtcg gttggtcggc gggatctcgt tgtcgtacca cgagtcgtcg
     1381 tcgtaacagt cggcgccgca gttgatgccg gtaccggtga ccgtgccacc gccgtggacc
     1441 tcaacggtga tctcgccgaa gacaagttcg tgttccggtt cggcctgagc cgtttgcgca
     1501 gtgaatgcga gggccagcaa ggccgccgcc aggacgagga acttcttcat tggattgctc
     1561 ctgttgattt tcgggggaaa ggggaaattc acgatggcgg caggtttccg gggagccctt
     1621 tccgtcttgt tgcggaaact ttgcggggtt cgcaaggttc gggccgaagc ccatccgaat
     1681 ttcaggtaga aagccgaaaa agccgaggca gacggctcgc tagtgtctgt gtatggccga
     1741 gcttcccgat cgtcaggaaa gcctcgaact ccttggtcgc atgtgcctga tccggcgctt
     1801 cgaggaggag gccggacgcc agtaccagcg ggccaaggcg ggcgggttcc tgcatctggc
     1861 gatcggcgag gaagcgacca tcgtcgggac cacctcggtc atgcgtgagg acgattacct
     1921 gatcggcacc taccggaccc acggtcacgc cctggcccgg ggcacccccg cgaacgaagt
     1981 catggccgag ctcttcggcc gtcagaccgg caccagcggc ggccggggcg gctcgatgca
     2041 catcttcgac gccgggcgcc gcttcatggg cggctacgga atcgtcggcg gcaacctgcc
     2101 cctcgccgcc ggcctcgccc tcgccagcca gtaccgggat gaggacacgg tcaccgtctg
     2161 catgttcggg gacggcgcct ccaacaccgg caacttcggc gagacaatga acctggcggc
     2221 gctctggaag ctgccggtcc tcttcctggt cgagaacaac ctttacggaa tgggcacctc
     2281 gcttgagcgc cactccgcgg tgaccgacct ctcccacaaa gcggccggat acggaatcga
     2341 gggcgagagg atcgacggca tgaacgtgct cgaggtgcgc gagcgggttg gcgaacacct
     2401 ggaaacagtc cggagtgaag gccggccgag cctggttgaa gcctttacct accgttatcg
     2461 cggccattcc gcggccgatc ccgaggttta ccgggagaag tccgaagtcg aagaatggcg
     2521 ggagaaggac ccgatcgaga acttcatccg gctctgttgc gatcaggggg tgctgagcga
     2581 cgacgacgtg gaagcggcca ggcgcgaggc cgacgagacc gtggaggccg cggtgaaatt
     2641 cgctgacgaa tcaccggagc ccgcgctcga atccctttac gaggagctct atgtcgtctc
     2701 ggagacgatg ggctggtacg ccgtcgacga gcgttcaccg gaaccccacc ggggcgagct
     2761 cggcgaggac gcccccgaac tggcacgcga cctcgccgaa tccggcgccg cccacgcgga
     2821 cggcgaggat ccggaacagc agtcccggcc gggggtgagg gactgatggc cgaactccgc
     2881 atgcgcgaag cgcttcgcga cgcgatggcg gaggaaatgc gccgtgacga atcggtattc
     2941 gtgatgggtg aagacgtcgg ggtcttccag ggggcgttca aggtgaccga gggcctgctc
     3001 gacgagttcg gcgagaagcg gatccgcgac acgccgatct cggagaacac gatcgtcggg
     3061 gtcggggtcg gggcggcgat ggccgggctg cggccggtgg tcgaactgat gaccgtgaac
     3121 ttttcgcttc tggccctcga ccagatcgtc aaccacgcgg cggcgatccc ctacatgttc
     3181 aacgggaacg tgcgggtgcc gatggtgatc cgcatgcccg gcgggggcgg ccaccagctc
     3241 ggccccaccc actcacacag tttcgaggcc ctctacctcc aggtgccggg acttctggtc
     3301 gcctgcccgt ccacgccggc cgacgccaag gccctgctca aggcgtcgat ccgggacgag
     3361 aacccggtca tcttcatcga gcacgagagc ctttacggga tcaagggcga ggtcccggaa
     3421 ggcgaggatg aaatcctccg gttcggcgag gcggcgatcc gccgcgaagg cagcgacgtg
     3481 accatcgtcg gcattctcaa gatggcccac gtggccgaac gggcggcggc ggaactcgag
     3541 gaagagcacg ggatctcggc cgaggtgatc gacccccgga ccctcaggcc gcttgatctc
     3601 gacacgatcc tcgcgtcggt aaagaagacc aaccgcgcgg tgatcgtcga ggagggctgg
     3661 ccgcacggcg gggtcggcgc gaacctttcg accctgatca ccgagcaggc tttcgactat
     3721 ctcgacgcgc cggtccagcg ggtgaccggc gccgacgtcc acatgcccta ctcccgccgc
     3781 atggaacagg cggcgattcc ccatgaggaa aacgtgatcg aggcggttct ggcgacgacc
     3841 gaaggggcat ttccgcccgc cggcggtaac tccggggaag gtgattcctg atggccgaga
     3901 tcgtcatgcc ccgcctgacc gactcgatgg aagagggcgt gatcctcgcc tggctgaagg
     3961 ctgacgggga cgaaatctcg gtcggtgacg agctggtcga gatcgagacc gacaaggcct
     4021 cgatggtcta cgaatccgac ctcgccggaa cccttgagat cctcgtcccc gaaggcgaaa
     4081 cccgcccgat cggggaaccg atcgcgaatg ttggtgacgg atcttcaagc cgtggtggtg
     4141 gcgcggtggc cgttggggat cccgtcggcc ggacttcgcc gggagttccg ccgggacccc
     4201 caac
//
LOCUS       DAOQKN010000191         4196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_12276_length_4196_cov_6.327940, whole genome shotgun sequence.
ACCESSION   DAOQKN010000191 DAOQKN010000000
VERSION     DAOQKN010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12276_length_4196_cov_6.327940"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="PLJ64_10815"
     CDS             complement(<1..964)
                     /locus_tag="PLJ64_10815"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HNC94017.1"
                     /translation="MTPSDPTPQQKLNPEQQEMMVESFFTRPAADRKIPELGVIPRAA
                     YNMVASEMELDGDPNKNLATFVTTWMEPEARQVITDSLHKNYIDHAEYPRTAEISKRC
                     VRMIHDLFNGPEGQDAPGTACAGSSEAVMLGALSMKWNWKNRQEKAGKDATRPNLVYG
                     SDVHVVWDKFCRYFDVEPRNIPIPKGKTVMGPDDFREHIDENTIGVIGVVGTTFTGQC
                     DDVVGIDQMLTEFKGKGLEVPMHIDAASGGFVFPFSHPDFEWDFRLDSVVSINVSGHK
                     FGLVYPGIGWLITRTDAQVAEDLIFYEDYLGEKDATFTLNFSGSS"
     gene            complement(1075..1911)
                     /locus_tag="PLJ64_10820"
     CDS             complement(1075..1911)
                     /locus_tag="PLJ64_10820"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HNC94018.1"
                     /translation="MAKAVSRGEEIGCRSIQIFNQSPRAWKPTEYSEEDFAEFREAMA
                     ASQVESVIIHAIYLINCVTPDRDLARKSRKALIHALRVGDGIGADGVVLHPGSRKQEE
                     VVPAIVRGGGLIEEALSESESCPLLIEQMAGHQNILAHTFEEIAGLIEAAGGDRRLGL
                     CLDSCHLFASGYDIGTYQGIDRMLKESDKAVGKRRLKALHLNDSKEPLGSRRDRHEDI
                     GKGEIGETGFARFLSEPRFEGLPATMETPGPDRKGPVAADIELAEKLKRRGRAARRRR
                     NS"
     gene            1940..3355
                     /gene="gltX"
                     /locus_tag="PLJ64_10825"
     CDS             1940..3355
                     /gene="gltX"
                     /locus_tag="PLJ64_10825"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNC94019.1"
                     /translation="MSIEKSGLRVRFAPSPTGALHIGGARTALYNWLAARHYGGDLVL
                     RIEDTDLERSTPENVAQILDALRWLELDWDEGPVSQVGRAEDHAAALQKLLDSGAAYR
                     DAATADDVKAWKSEHGADRGYRGTPSEDGSGAIRLRVPDDGDTVVNDLIRGEVRFPNR
                     SYDDFVIARGDGSVLYNFAVAVDDADMGITDVIRGDDHLSNTPKQLLVFEALGIQPPR
                     YAHVPLLHGPDGKKLSKRHGAASVQELRDAGYLPAAIRNYVALLGWGPGDDETLLSTA
                     ELIERFRPEDVGRSSAVFDEKKLRWMNGRYMRELPPGEYRESVRSFVEAETVGRPLNE
                     DRFAFGCEIVQEKAQTLQEVWPLIRFLFEPPVQDEKAWRKVMKGDAERVLVAVTEALQ
                     AVPAGGEGPNGDGFTIEAVQTALEPLPEKLELGAGKVFQPIRVSITGTTISPGIFESV
                     AALGREETIARIEAARSRLAA"
     gene            3435..3833
                     /locus_tag="PLJ64_10830"
     CDS             3435..3833
                     /locus_tag="PLJ64_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNC94020.1"
                     /translation="MISVTSKSRYAVVALVELAGAGDRPMPVKELAERRGIPDQFLEQ
                     LFSTLRRSGLLTSRRGSKGGYTLSRPASDITVLEVVQALDGKVGQEADEAGGIWAEGV
                     RALRGVFGETTIQDLYEREAEDAAARMYFI"
     gene            3830..>4196
                     /locus_tag="PLJ64_10835"
     CDS             3830..>4196
                     /locus_tag="PLJ64_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNC94021.1"
                     /translation="MIAPQASATVGGTPLVWLARIADGLDAQVCAKLEYFNPGGSVKD
                     RIGAAMIGAAEARGELVRGESVIVEPTSGNTGIALAMICAARGYQLVLTMPEGMSRER
                     SKLLRAYGAEVQETSSMGGM"
BASE COUNT          720 a         1325 c         1403 g          748 t
ORIGIN      
        1 acgagcttcc cgagaagttc agggtgaagg tggcgtcctt ctccccgagg tagtcctcgt
       61 agaagatcag gtcttcggcc acctgggcgt cggtccgggt gatcagccag ccgatcccgg
      121 ggtagaccag gccgaacttg tggcccgaga cgttgattga aacgaccgag tcgagccgga
      181 aatcccactc gaagtcgggg tgcgagaacg ggaagacgaa cccgcccgaa gcggcgtcga
      241 tgtgcatcgg cacctcgagg cccttgccct tgaactcggt cagcatctgg tcgatgccga
      301 ccacgtcatc gcactgaccg gtgaaggtag tgccgacgac gccgatcacc ccgatcgtgt
      361 tctcgtcgat gtgctcgcgg aagtcgtccg gccccatgac cgtcttgccc ttcggaatgg
      421 ggatgtttcg gggctcgacg tcgaagtagc ggcagaactt gtcccagacc acgtgaacgt
      481 ccgacccgta gacgaggttg ggccgggtcg cgtccttgcc ggccttctcc tggcggttct
      541 tccagttcca cttcatggaa agcgcgccga gcatgaccgc ctcggacgag ccggcacagg
      601 cggtgcccgg agcatcctgg ccttcgggac cgttgaagag gtcgtgaatc atgcgcacgc
      661 atcgcttgga gatctccgcg gtgcgcgggt actcggcgtg gtcgatgtag ttcttgtgca
      721 ggctgtcagt gatcacctgg cgggcttcgg gctccatcca ggtggtgacg aaggtcgcca
      781 ggttcttgtt cgggtcaccg tcgagttcca tctccgaggc gaccatgttg taggcggccc
      841 gggggatgac accaagctcg ggaatcttgc ggtccgcagc aggccgggtg aagaagctct
      901 cgaccatcat ctcctgctgt tcgggattca gcttctgctg gggtgtgggg tccgacgggg
      961 tcatctatct ccttcgacga tcggtttccc aaaaacctat ccgctgcctg tcactgtccg
     1021 ttcatgccga tcggatgatc cccgatccgg cgcggatgat caacggagca ggccctacga
     1081 attgcggcga cggcgagcgg cccgtcccct ccgcttcagc ttttcggcga gttcgatgtc
     1141 ggcggcaacc ggtcccttcc tgtccggccc cggggtctcc atcgttgcgg gcaggccttc
     1201 gaatctcggt tccgagagga atcgggcgaa tccggtttcg ccgatctcgc cctttccgat
     1261 gtcctcatga cggtcgcggc ggctgccgag cggctccttc gaatcattca ggtgaagagc
     1321 cttcaggcgc cgcttgccga ccgccttgtc cgactccttc agcatccggt caatgccttg
     1381 gtaggtaccg atgtcgtacc ccgacgcgaa aagatggcag gaatccaggc agagcccgag
     1441 tcgccggtcg ccgccggccg cctcgatcaa tccggcgatc tcctcgaagg tgtgggcgag
     1501 gatgttctgg tggccggcca tctgttcgat caagagcgga cagctttcgc tctccgaaag
     1561 ggcctcttcg atcaaaccgc cgccccggac gatcgccggc acaacctctt cctgcttgcg
     1621 tgaaccgggg tggaggacca cgccgtcggc cccgattccg tcgccgaccc gcagcgcgtg
     1681 gatcagcgcc ttgcgcgact tcctggccag gtcacggtcc ggcgtgacgc agttgatcag
     1741 gtagatcgcg tggatgatca cgctctcgac ctgactcgcg gccatcgcct cgcggaactc
     1801 ggcgaagtcc tcctccgagt attcggtcgg cttccaggcc cgcggactct ggttgaagat
     1861 ctggatcgag cggcagccga tctcttcccc ccgggacacg gccttcgcca agccgccgga
     1921 agtggaaaca tgggcaccga tgagcattga gaaaagcggc ctccgggtca gattcgcacc
     1981 ttctcccacc ggcgcactac acatcggggg agccagaacc gcactgtaca actggctggc
     2041 cgccagacat tacggaggcg acctcgtgct ccggatcgag gacaccgacc tcgagcggtc
     2101 gaccccggag aacgtggccc agattctcga tgccctgcgc tggctggagc tcgactggga
     2161 cgaaggtccg gtgtcccagg tcgggagggc cgaggaccac gccgcggcac tccagaagct
     2221 gctcgattca ggtgccgcgt accgtgacgc cgccaccgcc gatgacgtca aggcctggaa
     2281 gagcgaacac ggggcggacc gggggtaccg gggaacccct tccgaagatg gctccggcgc
     2341 gatacggctg cgggtgcccg atgacggtga caccgtcgtc aatgacctga tccggggcga
     2401 agtcaggttt cccaaccgca gttacgacga cttcgtgatc gcccggggcg acggttcggt
     2461 gctctacaac ttcgcggtcg cggtcgatga cgccgacatg gggatcaccg acgtgatccg
     2521 cggggacgat cacctctcga acaccccgaa gcaactgctc gtcttcgaag cgctgggaat
     2581 ccagccgccc cgctacgccc acgtgcccct gctgcatggt cccgacggca agaagctctc
     2641 gaagcgccac ggtgccgcct cggtccagga gcttcgtgac gcgggttacc tgccggcggc
     2701 gatccgcaac tacgtcgccc tgctgggctg ggggcccggc gacgacgaaa ccctgctctc
     2761 gaccgcggag ctgatcgagc gattccggcc cgaggacgtg ggcaggtctt cggcggtctt
     2821 cgacgagaag aagctccggt ggatgaacgg ccgttacatg cgggaactgc cgcccggcga
     2881 ataccgggaa tcggtgcgct ccttcgtgga agcggagacc gtcggccgtc cgctgaacga
     2941 ggatcgcttc gccttcggct gcgagatcgt ccaggagaag gcgcagaccc tgcaggaagt
     3001 ctggcccctg atccggtttt tgttcgaacc accggtccag gacgaaaagg cctggcgcaa
     3061 ggtgatgaag ggcgacgccg aaagggtgtt ggttgcggtc acggaggcgc ttcaagcggt
     3121 tccggccggt ggcgagggcc cgaacggaga cggcttcacg atcgaggcgg tccagacggc
     3181 actcgagccg ctgccggaga agctcgaact gggagccggc aaggttttcc agccgatccg
     3241 ggtttcgatc acgggaacga ccatctcgcc cggaatcttc gaatcggttg ccgctctggg
     3301 ccgggaagag accatcgccc ggatcgaagc ggcccgctcc cgactggctg cgtagtgccc
     3361 cattcctgac aaatacctgt ttcctgaccg ggttactaag tttttgacgg gctgatgcgg
     3421 taaaaagggg agcggtgatc tcggttacct ccaaatcccg ctacgccgtg gtcgctctgg
     3481 tcgagctggc cggagccggt gaccggccga tgccggtcaa ggaactggcc gagcggcggg
     3541 gaatcccgga tcagttcctc gaacagctgt tttcgaccct gcggcgctcc ggcctcttga
     3601 ccagccgtcg cggcagcaag ggcggctaca ccctttcccg cccggccagt gacatcaccg
     3661 tgctggaggt ggttcaggcg ctggacggca aggtcgggca ggaagcagac gaggccggtg
     3721 gcatctgggc cgaaggagtc agggccctga ggggcgtttt cggcgagacc acgattcagg
     3781 acctgtacga gcgggaagcc gaagacgccg cggcgaggat gtacttcatt tgatcgcccc
     3841 gcaggcatcg gcgacggtcg gcgggactcc gctggtctgg ctggcccgga tcgcggacgg
     3901 gctggatgcc caggtctgcg ccaagctcga gtacttcaat ccgggcggct cggtcaagga
     3961 ccggatcggc gcggcgatga tcggggccgc cgaggcgagg ggagagctgg tccgcggcga
     4021 gtcggtgatc gtcgagccga ccagcggcaa caccgggatc gccctggcga tgatctgtgc
     4081 cgcccggggc taccagctgg ttttgaccat gccggaaggc atgagtcgcg aacgcagcaa
     4141 gctgcttcgc gcctacgggg ccgaggttca ggaaacgtcg tcgatgggcg gcatgg
//
LOCUS       DAOQKN010000192         4165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_12439_length_4165_cov_4.860341, whole genome shotgun sequence.
ACCESSION   DAOQKN010000192 DAOQKN010000000
VERSION     DAOQKN010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4165
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12439_length_4165_cov_4.860341"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3298
                     /locus_tag="PLJ64_10840"
     CDS             <1..3298
                     /locus_tag="PLJ64_10840"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC94022.1"
                     /translation="PGRLEGRSELELALGDDAWVDWEAAQAGIEEARLDESAGRHREA
                     YELAGEAEKILSGGLLPGLEAGWIDEYRNELDDLRLQALELIARSGSQLGEAERARGE
                     RSARMAVEAAPFRESARTALIEVMEAQGNVAEALRAYDEFRVLLRDELGTFPAPELNA
                     VHERLLNAHEGVAAEKDTGSQTASPEAAAAVPAERQATPEQIGRLIDPRIGEIGLVGR
                     EEILAQLNHELDLAVAGDLRIALLAGDGGMGKTRIAAELAAGRDDVTVLYGRSEPDEI
                     RPFRIWSGLLRSAMRQAGDIPPAEIVGGDGPTLARILPELVRSMELPAPGPAGDLESE
                     RRALFGAVMRMIGRLTARQPMLIVLDDLHWADRSTLMLLASLAGDNPPGGVLALGIYR
                     DTELPEDSLLPETLTNLQRRLPTLKLEVEALGSEDVGQLIGGRLDAALAGSLHDQTGG
                     NPFLIEQLVRHLEETGIGDPGNAPAEVRQIISQRVNHLPDGGPDLLRRAALIGRDFDL
                     SILLETTIWDEDSVIDLLDAAVAAGLLDESPTVPGRYSFVHALLRSTLEEELGLTRRA
                     MIHRDIGEAIERQTASRPEKRVGEKAWHFTQAGPAEADRAVHWATQAAEQAEARLAYD
                     EAVDFFDGAIAAARADEPVDQGRLARLLLSQAKAKWKNGALQAAGDTFLEAAKAARES
                     GLPELAAQAAIGSRWGGWDAFDADLAEQLSLMRQALGSLPPVDSPLRAELMAMLGHTL
                     YYGSGQADEARQLAAEAIEMISRIDAPEAEFGVIQGTAFLRWQPLNRDQRLPASERML
                     EIAERLDDHELIGQALVSRQLSLLNLGRGAEAEADRSRHYAMSETLPQIRVISKAMRA
                     CSWFMTGDWQDGEDLFSELEAEGMPRGAALLLEDALRFMVRAQQGALAEHLGRLELFE
                     AGAKGWEMWPTWRLGLLLAKAQAGQLDEVRELIAEKGYESLDETRDINATLLPFCAVG
                     NMLAGELEDRDGAAKLVDLLTPFSGEWIMIARIGSTLGPVDMHLGELHLLCGEHREAA
                     TALERSLVACEAMDAVPYLARTKLALASAFGEMGDPDGQERRMELRNEGEQVAKRLDM
                     QAILRRHIRS"
     gene            3340..>4165
                     /locus_tag="PLJ64_10845"
     CDS             3340..>4165
                     /locus_tag="PLJ64_10845"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HNC94023.1"
                     /translation="MANPEITVCGRLTVAWDGEQLEGELPGRQGRLIFAYLVLNRSRP
                     VRRDELVEALWADEGLPSGGEALLAPPLSRLRKALGKDRLQGRADLSLQLGEEARIDF
                     EVAQQKLASAQNVAGNPDATADQLEAGWDDSREAAGILEGGLLPGLEARWIEEHRLYF
                     EELRLKSLETVARIGARLGTAQQAQAERAARNAVEASPFRESARAALIELLEAQGNIA
                     EALRAYEEMRVLLRDELGTFPAPELTAIHERLLNAHETPAAATGAPAPARPAPAPKP"
BASE COUNT          702 a         1435 c         1464 g          564 t
ORIGIN      
        1 cccggggcgg ctcgagggcc gctccgaact cgagctcgcc ctcggcgatg atgcctgggt
       61 cgattgggag gcagcccagg ccgggatcga agaggctcgc ctggatgaat cggccggtcg
      121 gcaccgggag gcctacgagc tggcgggcga agccgagaag atcctttccg gcgggctgtt
      181 gcccgggctc gaggcgggct ggatcgacga ataccgcaac gaactcgacg acctgcggct
      241 gcaggcgctg gagctgatcg ccaggtccgg cagccagctc ggcgaggccg aacgggcgcg
      301 cggggaacgg tccgcacgga tggcggtgga ggccgcgccg tttcgcgaat cggcccgcac
      361 cgcgctgatc gaagtgatgg aagcccaggg caacgttgcc gaagcgctcc gggcctacga
      421 cgagttccgg gtcctgctcc gcgacgaact gggaaccttt cccgccccgg aactgaatgc
      481 cgtccacgag cggctgctaa acgcacatga gggcgtcgcc gcggaaaagg acacgggctc
      541 gcagactgcc agcccggagg ctgcggccgc ggtcccggct gaacggcagg caaccccgga
      601 gcagatcggc cggctgatcg acccccggat cggcgaaatc gggctggtcg gccgcgagga
      661 gatcctggcc cagctgaacc atgaactgga cctggcggtc gcgggtgacc tgcggatcgc
      721 cctgctggcc ggcgacggcg gcatgggcaa gaccagaatc gccgccgagc tggccgccgg
      781 gagggatgac gtgaccgtcc tctacggccg ctcggaaccg gatgagatcc gccccttccg
      841 aatctggtcg ggcctgctcc gctcggcgat gcggcaggcc ggggacatcc ccccggccga
      901 aatcgtcggc ggcgacggcc cgaccctggc ccggatcctg ccggagctgg tccgcagcat
      961 ggaactgccg gccccgggac cggccggtga cctcgagtcc gagcgccggg ccctgttcgg
     1021 cgcggtgatg cgaatgatcg gccggctgac cgcccggcaa ccgatgctga tcgtgctcga
     1081 cgaccttcac tgggcggatc gctcgaccct gatgctgctg gccagcctgg ccggcgacaa
     1141 cccgcccggc ggggtgctcg ccctgggcat ctaccgggac acggaactgc ccgaggacag
     1201 cctgctgccg gagaccctga ccaacctcca gcgccggctg ccgaccctga agctggaagt
     1261 tgaagcgctc ggaagcgaag acgtcggcca gctgatcggc ggccgactgg acgcggccct
     1321 ggccggttcg cttcacgacc agaccggcgg caaccccttc ctgatcgaac agctggtccg
     1381 gcacctcgag gagaccggca tcggcgaccc cggaaacgcc ccggccgagg tccggcagat
     1441 catctcccag cgggtcaacc acctgcccga cggcggcccc gacctgctcc gccgggccgc
     1501 cctgatcggc cgcgacttcg acctttcgat cctcctggag acgaccatct gggacgagga
     1561 ctcggtgatc gacctgctcg acgccgccgt cgccgccggg ctgctcgacg aatccccgac
     1621 cgtccccggc cgctactcgt tcgtacacgc cctgctccgc agcaccctgg aggaggaact
     1681 ggggttgacc cgccgggcga tgatccaccg cgacatcggc gaagcgatcg aacgccagac
     1741 cgcgagccgc ccggagaagc gggtcgggga gaaggcctgg catttcaccc aggcggggcc
     1801 tgccgaagcc gaccgggccg tccactgggc gacccaggcg gctgaacagg cggaagcgcg
     1861 gctcgcctac gacgaggcgg tcgacttctt cgacggggcg atcgccgcgg cccgggccga
     1921 cgagccggtc gaccagggac ggctggcccg gctgctgctc agccaggcca aggcgaaatg
     1981 gaagaacgga gccctgcagg cagccggcga caccttcctc gaggcggcga aggcggcccg
     2041 ggaatcaggt ctgcccgagc tggctgccca ggcggcgatc ggcagccgct ggggcggctg
     2101 ggatgccttc gacgccgatc tggccgagca gctctcgctg atgagacagg ccctgggaag
     2161 cctgcccccg gtggacagcc cgctccgggc cgaattgatg gcgatgctgg gtcacaccct
     2221 ctactacggc agcggccagg ccgacgaagc ccggcagctg gccgctgagg caatcgagat
     2281 gatctcccgc atcgacgctc cggaagccga attcggcgtg attcagggaa ccgcctttct
     2341 gcgctggcag ccactcaacc gggatcagag actcccggcc tcggagcgga tgctggaaat
     2401 tgccgagcgt ctcgacgatc atgaattgat cggccaggcc ctggtctccc ggcagctttc
     2461 cctgctcaac ctcggccgcg gtgccgaagc cgaagccgat cgcagtcgcc actacgcgat
     2521 gagcgaaaca ctgccgcaga tccgggtgat ttcaaaggca atgcgggcct gctcctggtt
     2581 catgaccggc gactggcagg acggcgagga cctcttcagc gaactcgagg cggaagggat
     2641 gccccgcgga gccgccctgc tcctcgaaga cgcgctccgc ttcatggtcc gggcgcagca
     2701 gggcgccctg gccgagcacc tcggccggct cgagctcttc gaggcgggcg cgaaaggctg
     2761 ggagatgtgg ccgacctggc ggctcggact gctgctcgcc aaggcccagg ccggccagct
     2821 cgatgaagtt cgcgaactga tcgccgagaa gggctatgaa agcctggatg agacgaggga
     2881 catcaacgcg accctgctcc ccttctgcgc ggtcggcaac atgctggccg gcgagctgga
     2941 ggaccgcgac ggggcggcga agctggtcga tctgctcacc cccttcagcg gcgaatggat
     3001 catgatcgcc cggatcgggt cgactctcgg gccggtcgac atgcacctcg gggagctcca
     3061 cctcctttgc ggagagcatc gcgaggcagc taccgcgctg gaacggtcgc tggtggcctg
     3121 cgaggcgatg gatgccgtcc cctacctcgc gcgaaccaaa ctggccctcg cctcagcctt
     3181 cggggaaatg ggcgatccgg acgggcagga acgaaggatg gagcttcgaa acgagggcga
     3241 gcaggtcgcg aagagactcg acatgcaggc gatcctcagg cgtcacatcc ggtcctgaac
     3301 cgattcgcgc cgaccgggcc ggttggataa gttgccgcag tggcaaatcc ggaaataacg
     3361 gtttgcggcc ggctcaccgt ggcatgggat ggtgagcaac tggaaggaga gcttcccggc
     3421 cgtcaggggc gactgatttt cgcctacctg gtgctgaacc gttcccgacc cgtgcgcagg
     3481 gatgaactgg tcgaagcgct atgggccgac gagggcctgc ccagcggcgg cgaggcgttg
     3541 ctggcgcctc cgctctcccg gctgcggaag gccctgggca aggaccggct gcagggccgg
     3601 gccgaccttt cgcttcagct cggggaggaa gcacggatcg acttcgaggt tgcccaacag
     3661 aagctcgcct cggcccagaa cgtggccggg aacccggatg cgacagcgga tcagctcgaa
     3721 gccgggtggg acgactcccg tgaagccgcc ggcattctcg agggcggcct gctgcccggc
     3781 ctggaagccc gctggatcga ggagcaccgg ctctacttcg aggagcttcg cctcaagtcg
     3841 ctcgaaaccg tggcccggat cggggcccgc ctcggaaccg cccagcaggc ccaggccgaa
     3901 cgggcagccc ggaacgcggt agaagcctcc ccgtttcggg aatcagcccg ggccgcgctg
     3961 atcgagctgc tcgaagccca ggggaatatc gccgaggcgc tgcgggccta cgaggaaatg
     4021 cgggtgctgc ttcgcgacga gctcggcacc ttcccggctc cggagctgac cgcgatccac
     4081 gagcgcctcc tgaacgccca cgagactccg gccgccgcga ccggggcccc ggccccggcc
     4141 cggcccgccc cggcgcccaa acctg
//
LOCUS       DAOQKN010000193         4159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_12476_length_4159_cov_6.096735, whole genome shotgun sequence.
ACCESSION   DAOQKN010000193 DAOQKN010000000
VERSION     DAOQKN010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12476_length_4159_cov_6.096735"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="PLJ64_10850"
     CDS             <1..713
                     /locus_tag="PLJ64_10850"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNC94024.1"
                     /translation="ERDRAIRDAEERLRRVEQRAEDAERRAAFAERLAQLKVEESERE
                     KRLNDVVTGIDRAEQRAREAEARAAAAEQAAAAALEHSDLSPEPVSAPEPAAPAPVAP
                     TPEPSAPAPGPPLAEPYGVAPSAPEPVSEPAAPEPVFPEPVSAPAPAADEPPAPSRKG
                     LFGGSAPAATGGASDVVNLNTATFEELREAGLSVTQATRILAYRERFGGYGSVEDLEK
                     VPGFPADLIESLQGRITI"
     gene            complement(775..1374)
                     /locus_tag="PLJ64_10855"
     CDS             complement(775..1374)
                     /locus_tag="PLJ64_10855"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNC94025.1"
                     /translation="MKILAFSDLHRDLDQAARLTEMSRQADVVIGAGDFASIHQGLEE
                     TIDALAGIETPTVLVPGNNETLDALKEATAGWSAATVLHGEGIELDGKTFYGLGGGIP
                     VTPWDWSFDLEEADAGNMLAGLPAGAVLVVHSPPKGHCDESGNGMSLGSTAILKAIEE
                     KQPQLAVCGHIHEAWGKESKIGPTEVVNLGPSGRYFDLD"
     gene            complement(1384..2757)
                     /locus_tag="PLJ64_10860"
     CDS             complement(1384..2757)
                     /locus_tag="PLJ64_10860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNC94026.1"
                     /translation="MSIHGFDVVVLGAGPAGEVFAGQSAEAGFRVALVEQHLVAGECS
                     YYACMPSKALLYPGEVLDEARRAPGVREAVTGGLDVSAVLERRDEVIHHLDDSSQLAW
                     LRERGIELFRGSGVIAGEREVRVGGDVLVADRAVAIATGSSASIPPIPGLPEAPIWNN
                     RQGTTASTAPESLIILGGGPVGCELAQAWSSLGSEVTIFEPEERVLVHEEPFAGDLVE
                     QALWDRHGVTMVTGTAVESVSTTDSGITVSAGGKEYTAAELMVATGRRPRTDRIGLES
                     IGVDGGAELETDDCYRVKGHDWLFAVGDVNGRAPLTHMGKYQAWVGVRALLGHEPENR
                     AELIGSPRVTFTNPQVAAVGKTLAEAEAEGIDAVAIDADTGSTAGASFLGRGVDGKCR
                     LVVDQATGLVVGATFVGFEVAEWLQAATVAIVGKVSMDELRHAVAPFPTRSEIWLRFS
                     EEWEARR"
     gene            2851..3215
                     /gene="ssrA"
                     /locus_tag="PLJ64_10865"
     tmRNA           2851..3215
                     /gene="ssrA"
                     /locus_tag="PLJ64_10865"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(3355..>4159)
                     /locus_tag="PLJ64_10870"
     CDS             complement(3355..>4159)
                     /locus_tag="PLJ64_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94027.1"
                     /translation="LAILVTGLFVAVGPAPAQSAVPGIKQTVAYKRLKNYVGFLQSKR
                     NVPVKTTRRKTYKTTLSNRRNNANTKVLSLYSRTLLKISREDDAKQRRDIKRIRQNQK
                     AQVQALNARQANRLAALQAKENAASARIYASYNLKIDPLANKRDRLRRQLARTTKPAK
                     RSALTKQINKLQVKINGLVDDRTTDLNGLANRFNLKAQNIRNQFGQLIANVKANAQKQ
                     IQQERNAWRQTFRTQVQAAKTRRDAQKDMVGSQAERGFSFISQMPPAGG"
BASE COUNT          645 a         1397 c         1381 g          736 t
ORIGIN      
        1 aggagcgcga tcgcgccatt cgtgatgccg aggagcgcct gcgccgggtc gagcaacgag
       61 ccgaggacgc ggagcgccgg gccgcctttg ccgagcggct cgctcagctc aaggtcgagg
      121 aatcggaacg cgagaagcgc ctcaatgacg tggtaaccgg aatcgaccgg gccgagcagc
      181 gtgcccgcga ggccgaggcc cgggccgccg ccgcggaaca ggctgccgct gccgccctcg
      241 aacacagtga tttgagcccg gaacctgtct ccgcgcccga accggcggcg cccgcaccgg
      301 tcgccccgac ccctgagcct tcggctccgg ctcccgggcc gccgctcgcc gagccctacg
      361 gcgtggcgcc ctcggctccc gagccggttt ccgaacctgc cgccccggaa ccggtcttcc
      421 ccgagccggt ctccgctccc gctccggccg cggacgagcc gccggcccct tcacgcaagg
      481 gtctcttcgg cggttcggcg ccggccgcca ccggcggcgc ctccgatgtg gtcaacctga
      541 acacggcaac gttcgaggag ctgagggagg ccggactttc ggtcacccag gcgacccgga
      601 tcctcgccta tcgcgaacgc ttcggcggtt acggctcggt cgaggatctc gagaaggtcc
      661 ccggattccc ggccgacctg atcgagagcc ttcagggccg gatcacgatc tgacagggag
      721 tcgggcgggg tccgattcgc cctccagggg tggtcgggca ggcccggttc gaggtcagtc
      781 caggtcgaag taccggccgg aggggcccag gttgacgact tcggtcgggc cgatcttcga
      841 ttccttgccc caggcttcgt gaatgtggcc gcagacggcc agttgcggct gcttctcctc
      901 gatcgccttg aggatcgcgg tcgaaccgag gctcatgccg ttgccggatt cgtcgcaatg
      961 gccctttggg ggtgaatgga ccaccagcac ggcaccggcc ggcaggccgg ccagcatgtt
     1021 cccggcgtca gcttcctcga ggtcgaagct ccagtcccag ggggtcaccg ggatgccgcc
     1081 accgagcccg tagaaggtct tgccgtcgag ctcgatcccc tcgccatgga gaacggtcgc
     1141 cgcggaccag ccggcggtgg cttccttcag ggcatcgagc gtctcgttgt tacccggaac
     1201 cagcacggtc ggggtctcga tcccggcgag ggcatcgatc gtctcctcca ggccctgatg
     1261 gatcgaggca aagtcgccgg cgccgatcac cacgtcggcc tgccgggaca tctcggtcaa
     1321 ccgggccgcc tggtccaggt cacggtgcag gtcactgaaa gcgaggatct tcatgtgcca
     1381 accttaccgc cgtgcttccc actcctcgct gaacctcaac cagatttcgc ttcgggtggg
     1441 gaagggagcg acggcgtgcc gcagttcgtc cattgaaacc ttgccgacga tcgcgacggt
     1501 cgccgcctgc agccactcgg caacctcgaa tccgacgaag gtggctccga ccaccagtcc
     1561 ggtcgcctgg tcgaccacca gccggcactt gccgtcgact cccctgccga ggaacgaggc
     1621 cccggccgtc gagccggtat cggcatcgat cgccaccgca tcgattccct ccgcctcggc
     1681 ctcggcgagg gtcttgccga ccgccgccac ctggggattg gtgaaggtca cccggggaga
     1741 gccgatcaac tcggcccggt tttccggctc gtgcccgagc agggcccgga ccccgaccca
     1801 ggcctgatac ttgcccatgt gggtgagcgg cgcccggccg ttcacgtcac cgacggcaaa
     1861 cagccagtca tgccccttca cccggtaaca gtcgtcggtt tccagctctg cgccgccgtc
     1921 gacgccgatc gattccagac cgatccggtc ggtccggggc cggcggccgg tcgcgaccat
     1981 tagttcggcc gccgtgtact ccttgccccc ggcggaaacg gtgattcccg aatccgtcgt
     2041 gctgaccgat tcaaccgccg ttccggtgac catggtcacc ccgtgacggt cccagagagc
     2101 ctgttcgacc agatcgccgg caaagggttc ctcatggacg agcacccgtt cttccggctc
     2161 gaagatggtt acctcggaac cgaggctgga ccaggcctgg gcgagctcgc agccgaccgg
     2221 accgcccccg aggatgatca gggattcggg tgcggtgctg gccgtcgtgc cctgccggtt
     2281 gttccagatc ggggcctcgg gcagaccggg aatcggcggg atcgacgccg aggagccggt
     2341 ggcgatggcg accgcccggt cggccaccag gacatcgcct ccgaccctga cctcgcgttc
     2401 accggcgatc accccggagc cgcgaaagag ttcgatcccg cgctccctga gccaggccag
     2461 ctggctggaa tcgtcaaggt ggtgaatcac ttcgtcgcgg cgttccagaa cggcggagac
     2521 gtccagaccg ccggtgaccg cctccctgac ccccggggcg cgcctggcct cgtccaggac
     2581 ctcaccgggg tagagcagcg ccttggacgg catgcatgcg taataggagc actcgccggc
     2641 aaccagatgc tgttcgacca gggcgaccct gaacccggcc tcggccgatt gaccggcaaa
     2701 gacctctccg gctggccctg ccccgaggac gacgacgtca aatccgtgga tgctcaaact
     2761 gcctccgctg gtcgatctgg gctcttgcct ggccggttgc tgctttgacc cggccagaga
     2821 gacaccgtac aatggaggag caccatccaa ggggacgaca ggcttcgacg ttgtcggacc
     2881 gtgcggaggg ttgcaggtcg aggatgtcga tggcctcgta aaaaatcggc aaaaaaccat
     2941 atacggcgac gaacactcgt acgccctcgc cgcctagctg atacctaggt aacgagagtc
     3001 ggcccgccct cggcccgtgg ggtggaaatc cggcttcaga aacgggctta ccccgaagga
     3061 gcgccttggc cggaggggga cattcaagag gctttgccat ccgcagtgcc cgccggcgcg
     3121 gaccccgggt ggcgagaaca aagcgccggc taagcctgta gacgcctttc gacacgcgac
     3181 tgcggacgcg ggttcaattc ccgccgtctc cactgaaacc cgctccggcg ggttttccag
     3241 tggcgacctt gcactctggt ctccactgtc tgcgaacgaa agaaaaaggc ccgcctggtg
     3301 agcgggccct ttcgccgagg ctgatttctg cccggatcgg gcggctgacg gtgttcagcc
     3361 acccgccggc ggcatctgcg agatgaagct gaagccccgt tcggcctggg agccgaccat
     3421 gtccttctgg gcatcgcgac gggtcttcgc cgcctggacc tgggtgcgga aggtctggcg
     3481 ccacgcattc cgttcctgct ggatctgctt ctgggcattc gccttcacgt tggcgatcag
     3541 ctgaccgaac tggttgcgga tgttctgcgc cttcaggttg aagcggttgg cgagaccgtt
     3601 gaggtcggtg gtgcggtcat cgaccagacc gttgatcttg acctggagct tgttgatctg
     3661 cttggtcagg gccgatcgtt tggccggttt ggtcgtgcgg gcgagctgcc tgcgcaaccg
     3721 gtcccgcttg tttgccagcg ggtcgatctt caggttgtag gaggcgtaga tccttgccga
     3781 ggcggcgttc tccttcgcct ggagcgcagc aagccggttt gcctgccggg cgttcagggc
     3841 ctggacctgg gccttctggt tctggcggat ccgcttgatg tcacggcgtt gtttcgcgtc
     3901 gtcttcgcgg gagatcttga gcagggtccg cgagtagaga ctcagcacct tcgtgttcgc
     3961 gttgttgcgc cggttcgaaa gcgtggtctt gtaggtcttg cgtcgagttg tcttgaccgg
     4021 gacgttgcgc ttcgactgga gaaagccgac gtagttcttc agccgtttgt aggcgacggt
     4081 ctgcttgatg ccggggacgg cgctctgggc cggcgcgggt ccgaccgcta cgaacagtcc
     4141 ggtgacgagg attgcgagg
//
LOCUS       DAOQKN010000194         4155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_12492_length_4155_cov_7.788780, whole genome shotgun sequence.
ACCESSION   DAOQKN010000194 DAOQKN010000000
VERSION     DAOQKN010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4155
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12492_length_4155_cov_7.788780"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PLJ64_10875"
     CDS             <1..253
                     /locus_tag="PLJ64_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNC94028.1"
                     /translation="VFVDRCVEALDAGELTAEEAAEAKWWCTELQKRTVDACVQLHGG
                     YGYMLEYPIARAYADNRVTRIYGGTTEIMKEIVGRSMGL"
     gene            complement(389..1342)
                     /locus_tag="PLJ64_10880"
     CDS             complement(389..1342)
                     /locus_tag="PLJ64_10880"
                     /inference="COORDINATES: protein motif:HMM:NF019352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HNC94029.1"
                     /translation="MAYVSFKAGLLAVLLAFAGLFTLTSTTRAEVVDVHLTARPATVK
                     IDDGVKLRAWTFNGTVPGPVIRATEGDTVRVTLTNSHKGMHMKCRGKKGKALVRCQRF
                     NFLHEAMMHSIDFHAARIAPNVAFRNVAPGESFTFEFVAGTPGVYMYHCATGPMLHHL
                     GMGMYGMIVVDPATPRPPAQEVFLVQSEFYGKVKGGYLHSSYEAMQSGTPAMVVFNGR
                     SYRYALNPIQVGVGEPVRIFFLDAGPSLFSAFHVVGTIFDSYEHDGIPDEAIHDVSTQ
                     VIAPGGAGVFELSFPEAGSYPFVSHSVIDMDRGAMGMFEAS"
     gene            complement(1509..2291)
                     /locus_tag="PLJ64_10885"
     CDS             complement(1509..2291)
                     /locus_tag="PLJ64_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HNC94030.1"
                     /translation="MNFTLEIWRQFDGADESEAGRFETFKVEGINPDASFLEMLDQLN
                     ERLSHEKKRTIAFDSDCREGICGACSLVIDGTPHGPQQVTSCQTYMRDFIDGQTIKVE
                     PFRANTFPVIRDLAVDRGALDRVIQAGGYISTTTGPQPEPNSMPVSPEVQERAMDAAI
                     CIGCGACVAACPNGAAMLFTGAKVTHLNILPQGQPERETRVIGMVEQMDQEGFGGCTN
                     YGECSRVCPQEISIDVIGMLNREYRKAKLSRPAKTDRAMTTQ"
     gene            complement(2288..>4155)
                     /locus_tag="PLJ64_10890"
     CDS             complement(2288..>4155)
                     /locus_tag="PLJ64_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HNC94031.1"
                     /translation="DKWQNWLDAHTLVGPRNRAGKKVIVIGTGLAGSSCAASLGAQGF
                     KVKSFCFQDTPRRAHSIAAQGGINAAKDYANEGDSAHRLFYDTIKGGDFRSREANVMR
                     LAQLSENIIDQAVAQGVPFNREYSGALATRSFGGVLVQRTFYTRGQTGQQLLLGAYSA
                     LEHQVAAGNVEVFNRHEMQDVVVIDGEARGVIVRNLVTGEIERHAADAVVLASGGYTN
                     VYYLSTNAMGSNVTATWRAHKRGAYFANPCYTQIHPTCIPQSGEYQSKLTLMSESLRN
                     DGRIWVPRKAGDDRPPAGIPEAERYYYLEERYPAFGNLAPRDIASRAAKVVCDDGLGV
                     GGTGRGVFLDFRDAIADQGKEAIERKYGNLFEMYTRITGDDPYETPMMIYPAPHYAMG
                     GLWVDYELQTTIPGLFAIGEANFSDHGANRLGASALMQCLADGYFISPHTVTNHLAKN
                     HEPVSTSDSAFDEVENDVRGHVQELMDVGGSTSAQAFHREMGHVMLDKCGMSRTAEGL
                     DEALAEVRAIKEEFWRDLRVDPGDEEFNQSLEYANRVSDFFELGELMIQDARRREESC
                     GGHFREEHQTDDGEALRDDEHFAKVTAWEYGGDNPVPHDEALNFEDIKLATRSYK"
BASE COUNT          686 a         1298 c         1426 g          745 t
ORIGIN      
        1 ggtgttcgtc gaccgctgcg tggaggcgct cgacgccggc gagctgaccg ccgaggaggc
       61 ggccgaggcc aagtggtggt gcaccgagct ccagaagcgc acggtggacg cctgcgtcca
      121 gctccacggc ggctacggct acatgctcga gtacccgatc gcccgcgcct acgccgacaa
      181 ccgcgtgacc cgcatctacg gcggcacgac cgagatcatg aaggagatcg tcggccgctc
      241 gatgggcctc taggcgcatc cggagcgcca caattcacag aacctgaagt tgccggcggg
      301 tagcgctggg aacttcgagg tctctgcccg gcagagttat acgccccggc ggctccttgg
      361 ccacgtcccg ccgccggggc gtcctccctc aactcgcttc aaacattccc atcgccccac
      421 ggtccatatc gattaccgag tgggatacga agggatagct gcccgcttcg ggaaacgaca
      481 gttcaaacac cccagcgcca ccgggggcaa tgacctgggt cgacacgtcg tgaatcgcct
      541 cgtcgggtat gccgtcatgt tcatacgagt cgaagatcgt gccgaccaca tggaaggcac
      601 tgaagagcga tggtccggca tccaggaaga agatcctgac gggctcgccg acgccgacct
      661 gaatcggatt cagtgcgtag cggtagcttc ggccgttgaa gaccaccatg gccggagttc
      721 cgctctgcat ggcttcgtat gagctgtgca gatagccacc cttgaccttg ccgtagaact
      781 cgctctggac gaggaagacc tcctgggccg ggggccgggg tgtggccgga tcgaccacga
      841 tcatcccgta catccccatg cccaggtgat gcagcatcgg tccggtggcg cagtggtaca
      901 tgtagacccc cggggtgccg gcgacgaact cgaaggtgaa cgactcgccc ggggcgacgt
      961 tgcggaaggc gacattcggc gcgatgcgtg ccgcgtggaa atcgatcgag tgcatcatcg
     1021 cctcatgcag gaagttgaag cgctggcaac gcaccagggc cttgcccttc ttcccccggc
     1081 atttcatatg catgcccttg tggctgttgg tgagagtcac ccggacggtg tctccctcgg
     1141 tggcgcggat gaccgggccc ggcaccgtcc cgttgaaagt ccaggcccgc aacttcactc
     1201 cgtcgtcgat cttcaccgtg gccgggcggg ccgtcaggtg aacgtcgacc acctcggcgc
     1261 gggtggtgga ggtaagggtg aacaggccgg cgaaggccaa caggaccgca aggagaccag
     1321 ctttaaacga gacgtaagcc atggagcgac cgtataccgg tcacctgact tggacaaccg
     1381 taagaactac ggaaccgggt tgcgggaaac tacgcactcc cgcgcccggc ggggcgcgcc
     1441 gtcagcgaca gacagcgttg aggttattcg ctccatgtgc ggataacttc aatgcagttg
     1501 gtgaggcgtt actgggtggt cattgcccgg tcggtcttgg ccgggcggct cagcttggcc
     1561 ttgcggtact cgcggttgag cattccgatt acgtcgatcg agatctcctg ggggcagacc
     1621 cgggagcact cgccgtaatt ggtgcagccg ccgaagccct cctggtccat ctgctcgacc
     1681 atgccgatca cgcgggtttc gcgctccggc tggccctggg gcaggatgtt caggtgggtg
     1741 accttggccc cggtgaagag catcgccgcg ccgttcgggc aggccgctac gcaggcgccg
     1801 caaccgatgc agatcgccgc gtccatcgcc cgctcctgga cttccggtga aaccggcatc
     1861 gagttcggtt ccggctgggg accggtggtg gtcgagatgt agccgccggc ctggatcacc
     1921 cggtcgagcg cgccgcggtc aaccgcgagg tcgcggatca ccgggaaggt gttggcccgg
     1981 aacggctcga ccttgatcgt ctggccgtcg atgaagtcgc gcatgtaggt ctggcaggaa
     2041 gtgacctgct gagggccgtg cggagtgccg tcgatgacca gcgagcaggc gccgcagatg
     2101 ccctcgcggc agtcggagtc gaaggcgatg gtgcgcttct tctcgtgact gaggcgctcg
     2161 ttgagctgat cgagcatctc gaggaaggac gcatcggggt tgatgccctc gaccttgaag
     2221 gtctcgaagc ggcccgcctc gctttcatcg gcaccgtcga actgacgcca gatctcaagg
     2281 gtgaagttca cttgtagctc ctcgtcgcca gcttgatgtc ctcgaagttc agagcctcgt
     2341 catggggaac cggattgtcg ccaccgtact cccaggcggt gaccttggcg aaatgctcgt
     2401 cgtcgcggag cgcctcgccg tcatcggtct ggtgctcctc gcggaagtgg ccgccgcagg
     2461 attcctcgcg ccggcgggca tcctggatca tcagctcgcc gagttcgaag aagtccgaga
     2521 cccggttcgc gtactcgagc gactggttga actcctcgtc acccgggtcg acccgcaggt
     2581 cccgccagaa ctcttccttg atcgcccgca cctcggccag ggcttcgtcg aggccttcgg
     2641 cggtacggga catgccgcac ttgtcgagca tcacgtgacc catctcgcgg tggaaggcct
     2701 gggcggaggt gctgccaccg acgtccatca gctcctgaac gtgaccgcgg acgtcgttct
     2761 cgacctcgtc gaaggcggaa tcggaagtgc tgaccggctc gtggttcttg gcgaggtggt
     2821 tggtcaccgt gtggggggag atgaagtagc cgtcggccag gcactgcatc agggccgagg
     2881 cgccgaggcg gttggcgccg tggtcggaga agttcgcctc gccgatcgcg aacaaaccgg
     2941 ggatcgtggt ctggagttcg taatcgaccc agagcccgcc catcgcgtaa tgcggggccg
     3001 ggtagatcat catcggcgtc tcgtacgggt catcgccggt gatccgcgtg tacatctcga
     3061 agaggttgcc gtacttgcgc tcgatcgctt ccttgccctg atcggcgatc gcgtcgcgga
     3121 aatcgaggaa gacgccgcgg ccggtgccgc cgaccccgag gccgtcatca cagaccacct
     3181 tggcggcgcg ggaggcgatg tcacgcgggg cgaggttgcc gaaggccggg tagcgctctt
     3241 ccaggtagta gtaccgctcg gcctcgggga tcccggccgg cggacggtcg tcaccggcct
     3301 tgcggggaac ccagatccgg ccgtcgttgc ggagcgactc gctcatcagg gtcagcttcg
     3361 actggtactc gccggactgc gggatgcagg tcgggtggat ctgcgtgtaa caggggttgg
     3421 cgaagtatgc gccgcgcttg tgggcccgcc aggtggcggt cacgttcgag cccatcgcgt
     3481 tggtcgagag gtagtagacg ttggtgtagc cgcccgaggc gagcactacc gcgtcggccg
     3541 cgtggcgctc gatctcaccg gtgaccaggt tgcgcacgat cacgccacgg gcttcgccgt
     3601 cgatgaccac cacgtcctgc atctcgtgcc ggttgaagac ctcgacgttg ccggccgcga
     3661 cctggtgctc gagggccgag taggcgccga gcagaagctg ctggccggtc tggccgcggg
     3721 tgtagaaggt gcgctggacc agcaccccgc cgaaggaacg ggtggcgagc gcgccgctgt
     3781 attcgcggtt gaagggcaca ccctgggcga ccgcctggtc gatgatgttc tccgagagct
     3841 gggcgagccg catcacattc gcttcgcggg agcggaagtc gccgcccttg atcgtgtcgt
     3901 agaagagccg gtgggcgctg tcgccctcgt tggcgtaatc cttggctgcg ttgatcccgc
     3961 cctgggcggc gatcgagtgg gcgcggcgcg gcgtgtcctg gaagcagaag ctcttgacct
     4021 tgaaaccctg ggcgccgaga ctggccgcgc aggaggagcc ggccaggccg gtaccgatca
     4081 cgatcacctt cttgccggcc cggttgcggg gaccgaccag ggtgtgggcg tcgagccagt
     4141 tctgccactt gtcgg
//
LOCUS       DAOQKN010000195         4132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_12597_length_4132_cov_5.870002, whole genome shotgun sequence.
ACCESSION   DAOQKN010000195 DAOQKN010000000
VERSION     DAOQKN010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12597_length_4132_cov_5.870002"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /gene="rpsB"
                     /locus_tag="PLJ64_10895"
     CDS             complement(<1..572)
                     /gene="rpsB"
                     /locus_tag="PLJ64_10895"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNC94032.1"
                     /translation="MSQVGIQELLEAGVHFGHQTSRWNPNMRRYIAGEIDGIHIIDLL
                     QTEEMLEEARRFVGELTSGGGKVLFVGTKKQASDVIEDWAGKSNMPFVNRRWLPGLLT
                     NFSVSSQRIKRMHEIRGLVESGQIELLPTKERMNMQAELAKLEFALGGVADMDRVPDA
                     VFITDLKSEEIALNEASRLRLPVIALVDSN"
     gene            complement(705..1424)
                     /locus_tag="PLJ64_10900"
     CDS             complement(705..1424)
                     /locus_tag="PLJ64_10900"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNC94033.1"
                     /translation="MAPEGNRSGEPGPQGFRIVFTDEFLAVIDKGWDLVVHPAPSHEG
                     VTLVELLGDELAGGDDPERPGIVHRLDRGTSGLMIVAREEQTHADLQKMIRDRQVSRI
                     YSALADGRFRSRTGTIDAPIGRASRKRHRMAVNGAASRDAVTHFEVVEALRRESLLEV
                     RLETGRTHQIRVHLEAIGHPVVGDATYGGPDRYGLSRQFLHSSRLEFTHPRTGAEMSF
                     TAPLPEDLATALEAARGADSL"
     gene            complement(1414..1911)
                     /gene="lspA"
                     /locus_tag="PLJ64_10905"
     CDS             complement(1414..1911)
                     /gene="lspA"
                     /locus_tag="PLJ64_10905"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNC94034.1"
                     /translation="MRGYRHLIWAGLLALLVVILDQGAKALIVDNIDPGELVQVLGPL
                     DLTHSHNDGVAFGLAGGGGILVILLAAVALVALGAFIASAPESNLTWISGGLILGGAA
                     GNLIDRVRIGHVTDFILLPSWPAFNLADCAITVGVVLLAVSLIWGEQEDRKEPGAAGP
                     ETDGT"
     gene            complement(1913..2200)
                     /locus_tag="PLJ64_10910"
     CDS             complement(1913..2200)
                     /locus_tag="PLJ64_10910"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HNC94035.1"
                     /translation="MTRGDFAAYVNGLFTVYIILIFLRVLMSWIPRLPENRAIQAVAG
                     FIRETTDPYLNLFRRFLPPVGGGGMALDLSPVVGVILLLVAQQVVVALVRG"
     gene            complement(2222..2995)
                     /locus_tag="PLJ64_10915"
     CDS             complement(2222..2995)
                     /locus_tag="PLJ64_10915"
                     /inference="COORDINATES: protein motif:HMM:NF026098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase dimerization
                     domain-containing protein"
                     /protein_id="HNC94036.1"
                     /translation="MVVGFIGSGNMAAAMARGWASAEDDSPSEMLFTDSGSGRATALA
                     KEVGGRAVESNEELVRESDFVILAVKPAALELVAEKLPAPRAVLSLLGATPLEKLVGH
                     FPDSAVIRLMPNLGVEVNRGVVCMAEPEGVDEEILADTRKLLGSIARVHELPDESMDV
                     ATAVMGCSPAYFAVVAEALGSEGAHGGLEPDVAIKLVSESLAGTAALLGSRTPFEIQT
                     AVASPGGSTEAGLEALAAAGGADAFRAAFEASIARMRGN"
     gene            complement(3054..3617)
                     /gene="sepF"
                     /locus_tag="PLJ64_10920"
     CDS             complement(3054..3617)
                     /gene="sepF"
                     /locus_tag="PLJ64_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HNC94037.1"
                     /translation="MALRDSWHRTLVYFGLAEEGDHHEYDDYEDDFVEEDEYESRRGR
                     RSRDANVRRLPTSRRGRHDEIDDIFADDDPMPAATPRSRTLRSVGGDNGGDDVQVHLV
                     IPRNFNDAQQVADQFKRKVPVILNLQTTDHELSKRLIDFASGLTYALDGGMQRIAEKV
                     FLLTPRNVEVSAEEKARLIDKGFFNQS"
     gene            complement(3819..>4132)
                     /locus_tag="PLJ64_10925"
     CDS             complement(3819..>4132)
                     /locus_tag="PLJ64_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HNC94038.1"
                     /translation="ASGIIDRAASEIGVRAAAIGPGIGPCCFEVGPEVEEAFAGLGPG
                     LMNGRNLDLPEVARRLLARAGVEEVESAGVCTYCDERFFSHRRDRGKTGRQAGIAWLN
                     G"
BASE COUNT          695 a         1390 c         1336 g          711 t
ORIGIN      
        1 cagttgctgt caaccagggc gatgaccggc aggcggaggc gcgaagcctc gttcagggcg
       61 atctcttcgg acttgaggtc ggtgatgaag accgcgtccg gaacccggtc catgtccgcg
      121 acgccgccga gggcgaactc gagcttggcc agctcggcct gcatgttcat gcgctccttg
      181 gtcggcagca gttcgatctg gccggactcg acgaggccgc ggatctcatg catgcgcttg
      241 atccgctgcg aggacacgct gaagttggtc agaaggcccg gcagccagcg ccggttgacg
      301 aacggcatgt tcgacttccc ggcccagtcc tcgatcacgt cggaggcctg cttcttggtg
      361 ccgacgaaga ggacctttcc gccacccgag gtgagttccc cgacgaagcg gcgtgcttcc
      421 tcgagcatct cctcggtctg caagaggtcg atgatgtgga tcccgtcgat ctcgccggcg
      481 atgtagcggc gcatattcgg gttccagcgg ctggtctggt ggccgaagtg gactccggct
      541 tccaggagtt cctggattcc tacctgggac atgcttctcc ttgaattggg gtgaaaaacg
      601 gatccgcccg ggccgttttc ggtggaatcc gcctcggaac cgttccgggc ggcaggagcc
      661 accgtcgtat cccgggggtt agggaatacg gacagcagcc cagtctagag ggaatccgct
      721 ccgcgtgcgg cttcgagcgc cgtcgcgagg tcctccggca acggtgcggt gaaggacatt
      781 tccgcgccgg tccggggatg cgtgaattcg agccttgacg aatgcaggaa ctggcggctc
      841 agcccgtacc ggtccggccc gccgtaggtc gcgtccccga ccaccgggtg gccgatcgcc
      901 tcgaggtgaa cccggatttg gtgggtccgc cccgtttcca gtcgcacttc cagcaggctt
      961 tcgcgcctga gcgcctcgac cacctcgaaa tgggtgaccg cgtcccggga tgcggcgccg
     1021 tttacagcca tgcggtgacg cttgcgtgaa gcgcggccga tcggcgcgtc aatggtcccg
     1081 gtgcgggatc gaaaacgccc gtcggcgagc gccgaataga tccgcgaaac ctggcggtcc
     1141 cggatcatct tctgcaggtc ggcgtgagtc tgttcctcgc gggcgacgat catcaggcca
     1201 ctggtgccgc ggtccagccg gtggacgatc ccgggccgct ccgggtcgtc gccgcccgcc
     1261 agctcatctc cgagcagttc gaccagggtc actccttcgt gcgagggtgc cgggtgcacg
     1321 accaggtccc agcccttgtc gatcaccgcc aggaattcgt cggtgaagac gatccggaaa
     1381 ccctgcgggc ccggctcacc ggacctgttt ccctcaggtg ccatcggttt ccgggccggc
     1441 cgccccgggt tccttgcgat cttcctgctc gccccagatc agcgagaccg cgagcagaac
     1501 cacgccgacc gtgatcgcgc aatcggccag gttgaaggca ggccagctcg gcagcaggat
     1561 gaagtcggtg acgtggccga ttcggacgcg gtcgatcagg ttgccggccg caccgcccag
     1621 aatcagcccc ccggagatcc aggtcaggtt gctttcgggc gccgaggcga tgaaggcacc
     1681 gagcgcgacc agggccacgg cggccagcag gatgaccagg atcccgcctc ccccggccag
     1741 tccgaaggcg accccgtcgt tgtgggagtg ggtcaggtcc agcggcccga gcacctggac
     1801 gagttctccc gggtcgatgt tgtcgacgat cagggccttg gccccctggt caaggatgac
     1861 gaccaacagc gccagcagac cggcccagat cagatgccgg taacccctca attcagcccc
     1921 gcaccagggc gacgacgacc tgctgtgcga cgagcaggag gatcacgccg accaccggac
     1981 tcagatcgag tgccattccg ccgccaccca ccggcggcaa aaagcgccgg aacaggttca
     2041 ggtacgggtc ggtggtttca cggatgaagc cggccaccgc ctggattgcg cggttctccg
     2101 ggagcctcgg aatccaggac atcaggaccc gcaggaagat gaggatgatg tagaccgtga
     2161 acaggccgtt gacgtaggcg gcgaaatcgc ccctcgtcac tgccaggatc gtttccacgg
     2221 ctcagttccc gcgcatccgc gcgatcgagg cctcgaaggc ggcacggaag gcatcggcgc
     2281 cgccggccgc ggccagggct tccaggccgg cctcggtgct gccgccaggc gaggcgaccg
     2341 cggtctggat ctcgaaaggc gtgcggctgc cgagcagcgc cgcggtgccg gccagcgatt
     2401 cgctgaccag tttgatcgcc acatccggtt cgagtccgcc gtgggcgcct tcgctgccga
     2461 gtgcttccgc gaccaccgca aaataggccg gcgagcagcc catcaccgcg gtggcgacgt
     2521 ccatgctttc gtccggcagt tcgtgaaccc gggctatcga gccgagcagc ttgcgggtgt
     2581 cggcgaggat ctcctcgtca acgccttcgg gttccgccat gcagacaacc ccgcggttca
     2641 cctcgactcc gaggttcggc atcagccgga tcacggcact gtcgggaaag tggccgacca
     2701 gcttttcgag cggtgtcgct ccgagcagcg aaagcacggc ccgcggcgcc ggcagctttt
     2761 cagccaccag ttccagggcg gccggtttga ccgcgaggat cacgaagtcg gattcccgga
     2821 ccagttcctc gttgctctcg accgcccgcc cgccaacctc cttggccagc gccgtggcgc
     2881 gtccggaacc ggagtcggtg aagagcatct ccgacgggga atcatcttcg gcgctcgccc
     2941 agccgcgcgc cattgccgcg gccatgtttc cggaacctat gaatccgaca accatgctgc
     3001 gaagaatatg gatatgcccc ggtgatccgc ccaccgggga tcccgcgaac cggtcaggac
     3061 tggttgaaga agcccttgtc gatcaaccgg gccttctctt ccgccgaaac ctcaacgttg
     3121 cgcggggtca gcaggaagac cttctcggcg atccgctgca tgccgccgtc aagcgcgtag
     3181 gtgagcccgg atgcgaagtc gatcaggcgc ttggaaagct cgtggtcggt ggtctgcaga
     3241 ttgaggatga ccggcacctt gcgcttgaac tggtccgcca cctgctgcgc gtcgttgaag
     3301 ttccgcggga tgaccaggtg aacctggacg tcatcgccgc cgttgtcgcc acccaccgag
     3361 cgcagcgtcc ggctgcgcgg cgtggctgcc ggcatcggat cgtcgtcggc aaagatgtcg
     3421 tcgatctcgt cgtggcgtcc gcgcctcgag gttggcaggc gacgaacgtt cgcatcacgg
     3481 gaacgacggc cacgacggct ttcgtattcg tcctcttcga cgaagtcgtc ctcgtaatcg
     3541 tcgtattcgt gatggtcgcc ttcttcggcg agaccgaagt aaacgagtgt gcggtgccag
     3601 gaatcgcgta gtgccatagg tatgcggtgt tttcggtccg gggaggctga ttcctcccgg
     3661 ttgggggtcc ggtcgcagca attatcactg aattacccgt tttgcagcga aaatcacgct
     3721 cgaaggcaac ggccacccat cccgtccgac ccgtggccca gaccgcgaaa gcccactttg
     3781 cgccacatac cggcacaaaa tgggcattcg ccgcccgcct aaccgttgag ccaggcgatt
     3841 ccggcctgcc ggccggtctt tccccgatcc ctccgatgcg agaagaagcg ttcgtcgcaa
     3901 tacgtgcaga cgccggccga ctcgacctct tccaccccgg cccgggccag cagccgccgc
     3961 gcgacctccg gcagatcgag attccgcccg ttcatcagcc ccgggccaag ccccgcgaag
     4021 gcttcctcga cctcgggacc gacctcgaaa cagcaaggac cgatccccgg gccgatcgcg
     4081 gctgcccgca ccccgatctc gcttgccgcc cggtcgatga tcccggaagc ca
//
LOCUS       DAOQKN010000196         4063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_12976_length_4063_cov_5.621257, whole genome shotgun sequence.
ACCESSION   DAOQKN010000196 DAOQKN010000000
VERSION     DAOQKN010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12976_length_4063_cov_5.621257"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="PLJ64_10930"
     CDS             <1..364
                     /locus_tag="PLJ64_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94039.1"
                     /translation="IFCVRANSYRQSAARFIASQRQSINGLRFVRKGRLRQVGRLYFR
                     QTFIVTAGRGRNAGKGQLVFDYAAADNTGTYCYQSSKLFMVPRGDYRRGIRQLIRIYH
                     SAGYFGPGLPKGPNGEPA"
     gene            complement(395..1894)
                     /gene="lysS"
                     /locus_tag="PLJ64_10935"
     CDS             complement(395..1894)
                     /gene="lysS"
                     /locus_tag="PLJ64_10935"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNC94040.1"
                     /translation="MSEAENQEAGTEEGQVEGFAGILAERREKLDRLRAAGVDPFPHS
                     FSPREEISEVIAAHEGLEAGVETEAVHRVAGRISGRRGHGKAAFIDLRDASGQIQLHA
                     KADVLGDQYALLDDLDLGDFVGVEGRAIVTRRGQLSLEVTSWQVLTKALRPPPDKFHG
                     LEDTETRYRRRELDLIANEESREIFRVRARTVSEVRRWLDDHGFFEVETPVLQPLYGG
                     ALARPFVTHHNALDRQLFLRIATELYLKRCVVGGIDKVYELGKDFRNEGISMKHNPEF
                     TMLEWYEAYADYSDTAAELEELVSGVAEKVLGKAEIERDGRTISLAAPWHRVTLRDAI
                     LEHSGIDIDELTDRDALSEAIGNEDETAGWGKLVDTVFSKRVEPRLVEPTFILDYPEE
                     LSPFAKQHRDKPGKVERWEAFIGGVEIANSFTELNDPDEQRRRFEQQAAEIDRGDEEA
                     QPFDESFIEALEQGMPPTGGVGLGIDRLVMMLTGAGSLREVVLFPAMRD"
     gene            complement(1919..2398)
                     /gene="greA"
                     /locus_tag="PLJ64_10940"
     CDS             complement(1919..2398)
                     /gene="greA"
                     /locus_tag="PLJ64_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNC94041.1"
                     /translation="MSRQSEITPEGLKKLEEEVEYLSTVRRREVADRIKEARDFGDIS
                     ENAEYDDAKNEQAQLEAQIVQLEERLRRSTVVEEGSAEKGEVSFGSIVHIKDQDSGDS
                     KKFQIVGSTEADLAQGKLSSESPIGAALIGGKVNQIVKIPAPRGPEKQFKITKIEAA"
     gene            complement(2504..3403)
                     /locus_tag="PLJ64_10945"
     CDS             complement(2504..3403)
                     /locus_tag="PLJ64_10945"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC94042.1"
                     /translation="MTSGAGLSDRVEAWRLKGADEEFRGRQIHACFQDGEGPLVVLLH
                     GFPTCSYDWRYLFELMPDRALLAFDFLGFGLSDKPRDHSYTLSWQADLTEELICRHWA
                     GKPAFICAHDFGASVATELLARDLADELRFELAGMLLFNGSILVERSNPTLAQKLLRG
                     PLGPLAARFSNEAFFRRQFASVFGGAHPLTDADATDYWSLMTFNDGNRLGHKLVSYMD
                     ERAIYADRWHGAVSRWKGDLHLAWGMQDPVAVPDILDGLLDMRPDLPVVRMPELGHYP
                     QVEDPDRFARILTQSLATSNSSL"
     gene            complement(3400..>4063)
                     /locus_tag="PLJ64_10950"
     CDS             complement(3400..>4063)
                     /locus_tag="PLJ64_10950"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HNC94043.1"
                     /translation="PVPKVRKTGAGAELLSDPELALELARAAGRGSDLPVTVKIRSGL
                     MPGDRSGFDLAVRLVEEAGVAAIGFHPRVAKQGHKGQPDYAMARELSETIEVPMIISG
                     GLSSAEAARRAYEESNADAVMIARGSLGYPWVFAELTGQEVEPPTRESVMDELAWVMD
                     RAEEHLGEVRAGRYLRKFYPWYLDQLDVPKGVRNELCQTAGLDRAREIVAGIRNGEPV
                     TL"
BASE COUNT          660 a         1340 c         1361 g          702 t
ORIGIN      
        1 catcttctgt gtccgcgcga acagttaccg gcagtcggcg gcgagattca tcgccagcca
       61 gcgccagagc atcaacggac ttcgcttcgt gcgcaagggc cggctgcgcc aggttggccg
      121 gctctacttc cgccagacct tcatcgtcac ggcgggccgg ggacggaacg ccggcaaggg
      181 ccagctggtc ttcgattacg ccgccgccga caacaccggc acctactgct accagagctc
      241 caagctcttc atggttccgc gtggcgacta ccggcgcggc atcaggcagc tgatccggat
      301 ctaccattcg gccggctact tcggaccggg cctgcccaag ggcccgaacg gcgaaccggc
      361 ctgagggtcg gggccacgcc ccgcggccgg tcgatcagtc ccgcatcgcc gggaagagaa
      421 ccacttcccg gagggagccg gcaccggtca gcatcatcac caggcggtcg atgccgagcc
      481 cgacaccgcc ggtcggcggc atgccctgtt ccagcgcctc gatgaatgac tcgtcgaagg
      541 gctgggcctc ttcgtcgccc cggtcgatct cggccgcctg ctgttcgaag cggcgccgct
      601 gctcgtccgg gtcgttcagc tcggtgaacg aattggcgat ctcgacaccg ccgatgaagg
      661 cctcccagcg ctcgaccttc cccggcttgt cccggtgctg cttggcgaag ggagaaagct
      721 cttccgggta atcgaggatg aaggtgggtt cgaccagccg gggctcgacc cgtttcgaga
      781 agaccgtgtc cacgagcttg ccccagccgg cggtctcgtc ttcgttgccg atcgcctccg
      841 aaagggcatc ccgatcggtc agctcgtcga tgtcaatccc cgaatgctcg aggatcgcgt
      901 cgcggagggt gacccggtgc catggcgcgg cgaggctgat cgttcggccg tcccgctcga
      961 tctccgcctt gccgagcacc ttttcggcga ccccggagac cagttcctcg agctcggccg
     1021 cggtgtcgct gtaatccgcg taggcctcgt accactcgag catcgtgaac tcggggttgt
     1081 gcttcatcga gatgccctcg ttgcggaagt ccttgcccag ttcatagacc ttgtcgatgc
     1141 cgccgaccac gcaacgcttg aggtagagct cggtcgcgat gcgcagaaaa agctgacggt
     1201 cgagagcgtt gtgatgggtc acgaaaggcc gggccagggc accgccgtaa agcggctgga
     1261 ggaccggtgt ctcgacctcg aagaatccgt ggtcgtcgag ccagcggcgg acctcgctca
     1321 cggtgcgggc acggacgcgg aagatctcgc gtgactcttc gttggcaatc aggtcgagtt
     1381 cgcggcggcg atacctggtc tcggtgtcct cgagcccgtg gaacttgtcc gggggcggcc
     1441 tgagtgcctt ggtcaggacc tgccaggagg tgacttcaag ggaaagctgc ccgcggcggg
     1501 taacgatcgc ccggccctcg accccgacga agtcaccgag gtcgaggtcg tcgagcagcg
     1561 cgtactgatc tccgagcacg tcggccttgg cgtggagctg gatctgtccg gacgcgtccc
     1621 gcaggtcgat gaaggccgcc ttgccgtggc cgcgacggcc gctgatccgg ccggcgaccc
     1681 ggtgcaccgc ttccgtttcg acgccggctt cgaggccttc gtgggcggcg attacctcgc
     1741 tgatctcctc acgaggagaa aacgaatgcg ggaagggatc cacccccgcg gcacgcaggc
     1801 ggtccagctt ctcgcgacgc tcggcgagga tgccggcgaa accctcgacc tggccctctt
     1861 cggtcccggc ctcctggttt tcggcttcgc tcatcgccgg ccctttccgg tcagtcgatc
     1921 aggcggcttc gatcttggtg atcttgaact gcttctccgg accgcgcggg gccgggatct
     1981 tgacgatctg gttgaccttg ccaccgatca gggcggcgcc gatcggcgac tcgctcgaaa
     2041 gcttgccctg ggcgaggtcg gcctcggtcg aaccgacgat ctggaacttc ttcgagtcac
     2101 ccgagtcctg atccttgatg tggacgatcg agccgaacga gacctcgccc ttctcggccg
     2161 aaccctcttc cacgacagtc gagcggcgca ggcgctcctc gagctggacg atctgcgctt
     2221 cgagctgggc ctgctcgttc ttggcgtcgt cgtactcggc gttctcggaa atgtcaccga
     2281 agtcacgggc ttccttgatc cggtcggcca cctcgcgacg gcgtaccgtc gacagatact
     2341 cgacctcttc ttcgagcttc ttcagacctt cgggcgtgat ttcagactgg cgactcacag
     2401 gtatcaacct ctcttgggaa ggccggcaat cgaagcccgg ccctgaaaat gacgaacgcc
     2461 ctgagcggca ccagggggta ccggcagggc agcggacggg cgattataac gaggagttgc
     2521 tcgtggccag ggactgggtc aggatccggg caaagcggtc cggatcctca acctgcgggt
     2581 aatgccccag ttccggcatg cggaccaccg ggagatccgg cctcatgtca agcaggccgt
     2641 cgaggatgtc gggtactgca accggatcct gcatgcccca ggcgaggtga agatcgcctt
     2701 tccatcggga aaccgcgccg tgccagcggt cggcgtagat cgcccgttcg tccatgtacg
     2761 agaccagttt gtgaccgagc cggttgccgt cgttgaaggt catcaggctc cagtaatcgg
     2821 tggcgtcggc atcggtcagc gggtgggcgc cgccgaagac cgaggcgaac tgccgccgga
     2881 agaaggcctc gttcgagaag cgggcggcca gcgggccaag cgggccgcgc agcagcttct
     2941 gggccagggt cgggttcgat cgttcaacca ggatcgagcc gttgaagagc agcatcccgg
     3001 ccagttcgaa gcgcagttca tcggccaggt cccgggccag cagctcggtc gcgaccgagg
     3061 ccccgaagtc atgggcgcag atgaaggcag gtttgccggc ccagtggcgg cagatcagtt
     3121 cctcggtcag gtcggcctgc caggagagcg tgtaggagtg gtcgcgcggc ttgtcggaaa
     3181 ggccgaaacc gagaaagtcg aaggcgagca gggcccggtc cggcatcagc tcgaacaggt
     3241 agcgccagtc ataggaacag gtcgggaagc cgtggagcag gacgaccagc ggcccttcgc
     3301 cgtcctggaa gcaggcgtgg atctggcggc cgcgaaactc ttcgtcggcg cccttgagcc
     3361 gccaggcctc gacccggtcg gagagcccgg ctccgctggt cacagggtca ccggctctcc
     3421 gttgcggatt ccggccacga tctcgcgggc ccggtcgaga ccggcggtct ggcagagctc
     3481 gttgcggacc cccttcggca cgtcgagctg atccaggtac caggggtaga acttgcgcag
     3541 gtagcgaccg gcccttacct cgccgagatg ctcctcggcc cggtccatga cccaggccag
     3601 ctcgtccatg accgattcac gggtcggggg ctcgacctcc tggccagtga gttcggcaaa
     3661 gacccagggg tagccgagcg agccgcgggc gatcatcacc gcgtccgcat tggactcttc
     3721 gtaggcccgg cgggccgcct cggcgctgct caggccgccg gagatgatca tcggcacctc
     3781 gatcgtttcc gaaagttcgc gggccatcgc gtagtcgggc tgacccttgt ggccctgctt
     3841 ggcgacccgg gggtggaagc cgatcgcggc caccccggcc tcctcgacca ggcggacggc
     3901 cagatcgaaa ccggagcggt caccgggcat cagcccggag cggatcttca ccgttaccgg
     3961 tagatcggag ccgcggccgg ccgcccgggc cagttccagc gcaagctctg ggtcggaaag
     4021 cagttcggcc ccggccccgg tcttgcggac cttcggcacc ggg
//
LOCUS       DAOQKN010000197         4008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_13299_length_4008_cov_5.350620, whole genome shotgun sequence.
ACCESSION   DAOQKN010000197 DAOQKN010000000
VERSION     DAOQKN010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13299_length_4008_cov_5.350620"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="PLJ64_10955"
     CDS             complement(<1..257)
                     /locus_tag="PLJ64_10955"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase"
                     /protein_id="HNC94044.1"
                     /translation="MTESVGNNRTASPVQALLERLHARHAGDLSGEVASYIPELAGAD
                     PEWFGISMVTADGSVYEVGDTRREFTIQSISKPLTFALALEA"
     gene            312..1718
                     /locus_tag="PLJ64_10960"
     CDS             312..1718
                     /locus_tag="PLJ64_10960"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNC94045.1"
                     /translation="MPKYAGVLPLADTTVDLARLQFATTSIYHFLFVPLTLGLTPLVA
                     VMQTLWHRTGDERWYRLTRFFGTLMLINFAIGVATGIVQEFQFGMNWSTYSSYVGDVF
                     GAPLAIEGLAAFFLESVFLGLWIFGWDLLPKRVHLAMIWLAAIGTWLSAYFILVANSW
                     MQRPVGYELNGDKAQLNDVGALLSNRFAIHAFEHTLLVGLSTAALFIFGIACWHLVKG
                     RNVETFRKAAALALIVGVPATGINLAVGSGFGIVTTDYQPMKIAGSEALWNTEQPAGF
                     SLFQIGGFSQDDQDPSVVIQIPKLLSFLATGSVDGKVEGMNQLQADYQQKYGPGDYLP
                     PVRVNYWSMRVMAYTGTLMFLLMALGALFYWRGTLERRRRFLWAAVGGIALPYVAATA
                     GWVLTEMGRQPWIVQGLLKTADANSPAVSSGQIIASLSVFVLLYTCLAVADVTLMRRY
                     ARVDPPGGDDSKLEAEPA"
     gene            1715..2776
                     /gene="cydB"
                     /locus_tag="PLJ64_10965"
     CDS             1715..2776
                     /gene="cydB"
                     /locus_tag="PLJ64_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HNC94046.1"
                     /translation="MSLETVWFILIAVLWSGYFVLEGFDFGVGMLLPVLGRSESDRSA
                     MLNTIGPIWDGNEVWLIVAGGATFAAFPLWYATMFSGFYLALLAILVCLILRVVSFEW
                     RGKGDTPRWLGFWTWVNAVTSCLAAVIWGVALSNLLQGLPIDSSQEFTGNFLDLFSPF
                     TVFAGLAFCLLFLFHGAGFLSLRTVGDLGQRSARAARRLAAPALLAGILFMVWCVATA
                     VAENDRGVLGPLVPAVAGSLALCGAALAARARRPGLSFTLGAAGIVGVIATLFTGLYP
                     RVMVSSTDLANSLTTGNAASSHYSLTVITIVAGCLLPVVLAYQSWSYWVFRHRVSEDG
                     FEQAGNPIEAIRQRTEKRR"
     gene            2824..3528
                     /locus_tag="PLJ64_10970"
     CDS             2824..3528
                     /locus_tag="PLJ64_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNC94047.1"
                     /translation="MSPAAARTTGGKPDSLPTAAVEDYAKAIYSLTGWGEETASTNDL
                     AERLGVKAGSVSAMIKKLDEAGLVERVPYHGVRLTADGTRVAMAVLRRHRLLELFLAE
                     VLDVPWDRVHAEAEVLEHALSEDLTDLISTKLGDPDFDPHGDPIPDRDLAIVETETTA
                     LADLAPGDSARFVRVSDSNPEMLAYLSRCGIAVGDQLELIERQPFDGPITVRIDSDSH
                     VLGLTLAQAMRVDHNG"
     gene            complement(3610..>4008)
                     /locus_tag="PLJ64_10975"
     CDS             complement(3610..>4008)
                     /locus_tag="PLJ64_10975"
                     /inference="COORDINATES: protein motif:HMM:NF025343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HNC94048.1"
                     /translation="HSASVPFLRDTGFERMLVHYFSAAWMLPEVLRMHRDLWGRYRTN
                     IQHRISAAARERGLAMDTRLAAITLTQLIDGLWLGWVMEESYSQEDCRVILRKWLCDQ
                     FREDPEQYPLTPDIDLTHFETTAPLPRITD"
BASE COUNT          626 a         1351 c         1279 g          752 t
ORIGIN      
        1 gcctccagcg ccagggcaaa agtcagcggc ttggagatcg actggatcgt gaactcccgc
       61 cgcgtgtccc ccacctcgta aaccgacccg tcggcagtaa ccatcgaaat cccgaaccac
      121 tccggatccg cccccgccag ctcggggatg tagctcgcca cctcccccga aagatccccc
      181 gcatgccgcg catgcaaccg ctccagcaac gcctgaacag gcgaggccgt gcggttattg
      241 ccgacggatt cagtcatgga taggtacttg ccatcgtatt gaccggctca aacaacggtt
      301 ctgtccggtt tgtgccgaag tatgcaggtg tgctgccgct ggctgacacg accgttgacc
      361 tggcgcggct tcagttcgcg accacctcga tctaccattt cctcttcgtt ccgctgaccc
      421 tcgggctgac cccgctggtg gccgtgatgc agaccctctg gcaccggacc ggggacgaac
      481 gctggtaccg gctgacccgc ttcttcggga ccctgatgct gatcaacttc gcgatcgggg
      541 tggcgaccgg aatcgtccag gagttccagt tcgggatgaa ctggtcgacc tactcgagct
      601 atgtcggtga cgtgttcggg gcgccgctgg cgatcgaggg tctggccgcc ttcttcctcg
      661 agtccgtctt ccttggcctg tggatcttcg gctgggacct gctgccgaag agggtccacc
      721 tggcgatgat ctggctggcc gcaatcggaa cctggctctc cgcctacttc atcctggtcg
      781 ccaattcatg gatgcagcga ccggtcggct atgaactgaa cggtgacaag gcccagctca
      841 atgacgtcgg tgccctgctg agcaaccgct tcgcgattca cgccttcgaa cacaccttgc
      901 tggtcggcct ctcgaccgcg gccctgttca tcttcggcat cgcctgctgg catttggtca
      961 agggcaggaa tgtcgagacc ttccgcaagg cggctgccct ggccctgatc gtcggagtgc
     1021 cggcgaccgg aatcaatctc gcggttggca gcggcttcgg gatcgtcacc accgattacc
     1081 agccgatgaa gatcgccggc tccgaggctc tgtggaacac cgaacaacca gccggcttct
     1141 cgctcttcca gatcggcggc ttcagccagg acgaccagga tccgtcggtg gtgatccaga
     1201 ttccgaaact gctctcgttc ctggccacgg gatcggtaga cggcaaggtc gaaggcatga
     1261 atcagctcca ggccgattac cagcagaagt acggccccgg cgactacctg ccaccggtcc
     1321 gggtcaacta ctggtcgatg cgggtgatgg cctacaccgg caccctgatg ttcctgctga
     1381 tggcgctggg ggcgctgttc tactggcggg gaaccctgga aaggaggcgg cgtttcctct
     1441 gggccgcggt cggcgggatc gcgctgccct atgtcgcggc cacggccggc tgggtgctga
     1501 ccgaaatggg ccggcagccc tggatcgttc agggcctgct gaagaccgcg gacgcgaact
     1561 ccccggccgt gtccagcggc cagatcatcg ccagtctctc cgtcttcgtg ctgctctaca
     1621 cctgcctggc tgtcgccgac gtgaccctga tgcgccgtta cgcgagggtc gacccgccgg
     1681 gcggcgacga ttcgaaactg gaggccgagc cggcatgagc cttgaaacgg tctggttcat
     1741 cctgatcgcc gtgctctgga gcggctactt cgtgctggaa gggttcgact tcggggtggg
     1801 gatgctgctg cctgtactgg gccggagcga gtccgaccgg agcgcgatgc tgaacacgat
     1861 cgggccgatc tgggacggca acgaggtctg gctgatcgtc gccggcggcg cgaccttcgc
     1921 cgcctttccg ctctggtacg cgaccatgtt ctccggcttc tacctggccc tgctggcgat
     1981 tctggtctgc ctgatcctca gggtcgtctc cttcgagtgg cgcggcaagg gcgacacgcc
     2041 ccgctggctc ggcttctgga cctgggtgaa cgcggtcacc agttgcctgg ccgcagtgat
     2101 ctggggcgtc gctctttcga acctgctgca gggtctgcca atcgattcct cgcaggagtt
     2161 caccggcaac ttccttgacc tcttctcacc cttcacggtc ttcgccgggc tcgcgttctg
     2221 cctgctcttt ctgttccacg gggccgggtt cctctccctg aggaccgtcg gcgatctcgg
     2281 ccagcggtcg gccagagcgg cacgccggct ggcggcgccg gccctgctcg ccggaatcct
     2341 cttcatggtt tggtgcgtgg caaccgcggt ggcggaaaac gaccgcggcg tcctcggtcc
     2401 gctcgtcccg gcggtggccg gatcactcgc tctctgcggc gcggcccttg ccgcccgtgc
     2461 ccgacgcccc ggtctctcct tcactctggg agcggccggc atcgtcggtg tcatcgccac
     2521 cctcttcacc gggctctacc cgcgggtgat ggtttcctcg accgacctcg ccaacagcct
     2581 caccaccggc aacgccgcct cttcccatta ctcgctcacg gtgatcacga tcgtcgccgg
     2641 atgccttctg ccggtcgtgc tcgcctacca gtcctggagc tactgggtgt tccgccaccg
     2701 ggtttccgag gatggcttcg agcaggccgg aaacccgatc gaggcgatca ggcagcggac
     2761 cgaaaagcga aggtaatccc ctcacccgat ccggggaccg caggttttgg ctaggctcaa
     2821 cctgtgagtc cagccgccgc caggacaacc ggaggaaaac ccgattcgct gccgaccgcc
     2881 gcggtcgagg actacgcgaa ggcgatctac tcactgaccg gctggggtga ggaaaccgct
     2941 tccaccaacg acctcgccga gcggctcgga gtgaaggccg gctcggtctc ggcgatgatc
     3001 aagaagctgg atgaagcggg gctggtcgag cgggttccgt accacggcgt caggctgacc
     3061 gccgacggca cgcgggtggc aatggcggtg cttcgccggc accggctgct cgaactattc
     3121 ctcgccgagg ttctggacgt gccctgggac cgggttcacg ccgaggcgga ggttctcgaa
     3181 cacgctctgt ccgaggatct caccgacctg atctcgacca agctgggtga ccccgacttc
     3241 gatcctcacg gcgacccgat cccggaccgg gacctggcga tcgtcgaaac cgagacgacc
     3301 gccctggccg acctcgcgcc cggtgattcg gcccggttcg tccgggtctc ggattccaac
     3361 ccggagatgc ttgcctacct cagccggtgc ggaatcgcgg tcggtgacca gctggagttg
     3421 atcgaacggc aaccctttga cggaccgatc accgtccgca tcgacagcga ctcgcatgtc
     3481 ctcggcctga ccctcgccca ggcgatgcgg gtcgaccaca acggctgagc aaccggggcg
     3541 aaccagctgg ccagccggat tcagcccggg gtcgggccgc cggggttgcc gggcccggtt
     3601 gcgccgggat cagtcggtga tccgcggcaa gggcgcagtg gtctcgaaat gcgtcaggtc
     3661 gatgtccggg gtcagcgggt attgctccgg atcctcgcgg aactggtcgc agagccactt
     3721 gcgcaggatc acgcggcaat cctcctgcga atagctctcc tccatcaccc agccgagcca
     3781 gagcccgtcg atgagctggg tcagggtgat cgccgccagc cgcgtgtcca tcgccagccc
     3841 gcgttcccgc gcggcggcgg aaatgcggtg ctggatgttg gtgcggtagc ggccccagag
     3901 atcgcggtgc atcctcagca cctcgggcag catccaggcg gcgctgaagt aatggaccag
     3961 catccgttcg aagccggtgt cgcgcaggaa cggcacgctg gccgaatg
//
LOCUS       DAOQKN010000198         3993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_13389_length_3993_cov_5.891315, whole genome shotgun sequence.
ACCESSION   DAOQKN010000198 DAOQKN010000000
VERSION     DAOQKN010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13389_length_3993_cov_5.891315"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="PLJ64_10980"
     CDS             complement(<1..513)
                     /locus_tag="PLJ64_10980"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="laccase domain-containing protein"
                     /protein_id="HNC94049.1"
                     /translation="MKWKEEAGLPWLEADLTGDATVCFSSRLGGVSQGPFESLNLGIL
                     TEDRREDVLANRRKLAVAAGFDADRVSMGRQVHGAELNWAGPGIPGAYSAPGPDPPPE
                     ADGQLTKETLRPVLVLVADCLPVALLGERGLGMLHCGWRGLASGIVDRAASEIGVRAA
                     AIGPGIGPCCF"
     gene            complement(530..1648)
                     /locus_tag="PLJ64_10985"
     CDS             complement(530..1648)
                     /locus_tag="PLJ64_10985"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94050.1"
                     /translation="MTPGIDPDFNRLATLIRTHPAIAPIREAATTPLYLVGGAIRDAL
                     ADFRVDDIDLVIEGDPVPLAAGFDPAAKVNDRFGTVNLLVDGYPVDIATARTETYSRP
                     GALPDVRPGTLEDDLRRRDFTINAMAIGIDEGSEMIDQFGGIADLESGVLRVLHDASF
                     EDDPTRALRAARYAARLDFDLEPKTAELMMKVNLGSISRDRVESELRLIALEDNALEA
                     LRLARLWGLVDFPEERLDLAEQAIELIEIEPWRDEAPREALILEVVFGNLSGAEELLG
                     EPGTPWQACRLTRGRTALELVVARALGAVWLDRWQREWRWVELEITGADLLAAGVPEG
                     PQIGAGLDAALEAKLDRGESGVEAEMAVALKAVGARLP"
     gene            1682..3073
                     /locus_tag="PLJ64_10990"
     CDS             1682..3073
                     /locus_tag="PLJ64_10990"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNC94051.1"
                     /translation="MTNTPINSPNGSGKPPWYRQRGKVISLIAVVLVAAGAIALVAWN
                     ELKRPDDVSNPDVPFVKTKPKQVVPKDRTINWSTFRLNPQRTGYLPVNGIHPPFKRVW
                     KYGDQPLLEFPPIVVDGRLYFVDNDGNARALRADNGREIWHRKIADLNASTPTYARGR
                     LYIVNLEPGHVLSMNAKNGRIIWEKPLPCRSESSPAVIRQRVYFGCENGVLYALKARN
                     GKEVWETSVDGAIKGAPAYEDGTLFVGDYGGVVSAVRAKTGEIKWQSDSLGGSFGRAG
                     SFYSTPAVAYGRVYLGSNDGRVYSFDKNTGELAWTYSTGDWVYSGPSVAQVPGTPPTV
                     YIGSFDGNVYALNARSGEARWTFPMGGQVIGSLSVIGRTVYAATFDGTTTYGISAKSG
                     RKVFEFHTGAYMPGISDGEKLYLVGYSSIHAMKPITLKQLRAERRAKEARKAARQKAR
                     KQAAQNQAGPSGQ"
     gene            3077..>3993
                     /locus_tag="PLJ64_10995"
     CDS             3077..>3993
                     /locus_tag="PLJ64_10995"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNC94052.1"
                     /translation="MDSGFEARLARRREEVRRRQIVRRRVVLGSMLGVLLAAAVFMLF
                     LRSDDSGENSTQQTAANNTAGQQGAGTSETLQTSGGTDASRGGAQPDDSWGPHTGPVP
                     ILMYHVIGPAAGSEDYPGLFLSTPDFQDQVDWLADNGYTAVTLVQVQNAWYDGGTLPE
                     RPVVLSFDDGYLGQYLFAMPILEKQGWGGQLNLKSEGSDLSSKQVKKMYRAGWEIASH
                     SITHPDLTTLDPATLEHELVGSKEELEKDLGFEIVNFCYPAGQYDDEVVKAVEAAGYR
                     GATTVNPGLAEKSMPFELNRIRIDAGDGP"
BASE COUNT          699 a         1407 c         1262 g          625 t
ORIGIN      
        1 gaaacagcaa ggaccgatcc ccgggccgat cgcggctgcc cgcaccccga tctcacttgc
       61 cgcccgatcg acgatcccgg aagccaggcc ccgccagccg cagtggagca tcccgaggcc
      121 cctttcaccg agcagggcga ccggcaggca gtcggcgacc agcaccagaa cgggtctcag
      181 ggtttctttc gtcagctggc cgtcagcctc cggcggcgga tcgggcccgg gggccgaata
      241 ggcaccggga atcccggggc cggcccagtt cagctccgcg ccgtgcacct gccggcccat
      301 tgagacccgg tcggcatcga aaccggccgc gaccgcgagc tttcgtcgat tcgcgagcac
      361 gtcctcccgc cggtcctcgg tgaggatgcc gagattgagc gactcgaacg gtccctggct
      421 gaccccgccc agcctgctgc tgaaacagac cgttgcgtcc ccggtcaggt cagcctcaag
      481 ccagggcaga ccggcttcct ctttccactt cacggcagcc ccgcggaagt cacggcaacc
      541 gggccccgac cgctttgagg gcgaccgcca tttccgcctc taccccgctc tccccgcgat
      601 ccagcttcgc ttccagggcg gcgtccaggc cggcgccgat ttgcggtcct tcgggaaccc
      661 cggcggccag caggtcggcc ccggtgatct cgagctccac ccagcgccat tcgcgctgcc
      721 aacggtccag ccagacggcg cccagcgcgc gggccacgac aagttccagc gcggtccgcc
      781 cccgggtgag ccggcaggcc tgccagggag ttccgggctc cccaaggagc tcctcggccc
      841 cgctcaggtt gccgaaaacg acctcgagga tcagcgcctc ccgcggggct tcatcccgcc
      901 agggttcgat ctcgatcaac tcgatcgcct gttctgcgag gtcgagccgc tcctccggaa
      961 agtcgacaag tccccagagc cgggccagcc gcagcgcttc cagggcattg tcctcgaggg
     1021 cgatcaggcg cagttcactt tcgacccggt cccgtgagat cgaaccgagg ttcaccttca
     1081 tcatcagctc ggccgtcttc ggttcgaggt cgaagtccag gcgggcggcg tagcgggccg
     1141 cccggagcgc ccgggtcggg tcatcctcga aggatgcgtc gtggagcacc ctgagcaccc
     1201 ctgactccag gtcggcgatg ccgccgaact gatcgatcat ctccgagccc tcgtcgatgc
     1261 cgatcgccat cgcgttgatc gtgaagtcgc ggcggcgcag gtcgtcctcg agggtgcccg
     1321 ggcggacgtc cggaagcgct ccgggacgcg agtaggtctc ggttcgggcg gtggcgatgt
     1381 caaccggata cccgtcgacc agcaggttga cggtgccgaa acggtcgttg accttcgccg
     1441 cggggtcaaa cccggcggcc agcggcaccg ggtccccttc gatgaccaga tcgatgtcat
     1501 cgacccggaa atcggcgagg gcgtcacgaa tcgcaccccc caccaggtac aacggcgtgg
     1561 tcgccgcttc acggatcggc gcgatcgccg ggtgggtccg gatcagggtg gcgagtcggt
     1621 tgaagtcagg gtcaattccg ggggtcatcg ggtcactccc gtaccatgat cccttcttcg
     1681 gatgaccaac acaccaatca actctccaaa cgggtcgggc aagcccccct ggtaccggca
     1741 gcgcggcaag gtcatttcgc tgatcgcggt ggtcctggtg gccgccggcg cgatcgccct
     1801 tgtcgcctgg aatgagctga agcggcccga tgacgtcagc aatcccgacg tcccgttcgt
     1861 caagaccaag ccgaagcagg tcgtgccgaa ggacaggacg atcaactggt ccactttccg
     1921 cctcaacccc cagcgcaccg gctatctgcc ggtgaacggc atccatccgc ccttcaagcg
     1981 ggtctggaag tacggcgacc agccgctgct cgagttcccg ccgatcgtgg tcgatggccg
     2041 gctttacttc gtcgacaacg acggtaacgc gagagccttg cgggccgaca acggacggga
     2101 gatctggcat cgaaagatcg ccgacctgaa tgcctcgacc ccgacctatg ccaggggccg
     2161 cctttacatc gtgaatctcg agcccggcca cgtgctttcg atgaacgcga agaacggccg
     2221 gatcatctgg gagaagcctt tgccctgccg ttccgaatcc tcgcccgcgg tgattcgcca
     2281 gcgggtgtac ttcggttgcg agaacggggt gctgtatgcg ctcaaggccc gaaacggcaa
     2341 ggaagtgtgg gaaacctcgg tcgacggggc gatcaaggga gcaccggcct atgaggacgg
     2401 caccctcttc gtgggcgact acggaggcgt cgtttcggcg gtgcgggcaa agaccggtga
     2461 aatcaagtgg cagtcggaca gcctcggcgg cagcttcggc cgggccggtt ccttctattc
     2521 gacaccggcg gtcgcctacg gccgcgtata tctcggcagc aacgacggcc gggtctacag
     2581 ctttgacaag aacaccggcg aacttgcctg gacctactcg accggcgact gggtctactc
     2641 gggcccgtcg gtcgcccagg ttccgggcac tccgccaacc gtctacatcg gctccttcga
     2701 cggcaacgtc tacgccctca acgcccggtc cggcgaggca cgctggacct tcccgatggg
     2761 cggccaggtg atcggttcgc tcagcgtaat cggccgaacg gtctacgccg cgaccttcga
     2821 tggcaccacc acctacggga tcagcgccaa gtccggacgc aaggtcttcg agttccacac
     2881 cggcgcctac atgccgggga tctcggatgg cgagaagctc tatctggtcg gctactcgag
     2941 catccacgcg atgaagccga tcacgctcaa gcagctgcgg gccgaacgcc gcgccaagga
     3001 agcccgcaag gccgcccgcc agaaggcccg caagcaggca gcccagaatc aggccgggcc
     3061 aagcgggcag tagccggtgg actccggttt cgaggcccgg ctggcccgcc ggcgggagga
     3121 ggtccgtcgc cggcagatcg tccgccgtcg cgtcgtgctc gggtcgatgc ttggggtgct
     3181 gctcgctgcg gcggttttca tgctcttcct gcgttccgac gactcgggcg agaacagcac
     3241 tcaacagacc gccgccaaca acaccgccgg acagcaaggc gccgggactt ccgagacgtt
     3301 gcagaccagc ggtggaaccg atgcatcacg gggcggcgcc caacccgacg acagctgggg
     3361 gccgcacacc ggcccggtgc cgatcctgat gtaccacgtg atcggccccg cagccggatc
     3421 cgaggactac cccggtctct ttctctccac acccgatttc caggatcagg tcgactggct
     3481 ggccgacaac ggctacaccg cggtcaccct cgtccaggtc cagaacgcct ggtatgacgg
     3541 cggcaccctg ccggagaggc cggtcgtcct ttccttcgac gacggctacc tcggccagta
     3601 cctgttcgcg atgccgatcc tcgagaagca gggctggggc ggccagctca acctcaagtc
     3661 ggagggctcg gacctctcct cgaagcaggt gaagaagatg taccgggccg gctgggagat
     3721 cgcctcccac tcgatcaccc accccgatct caccaccctc gatcccgcca ccctcgaaca
     3781 cgaactggtc ggctcgaagg aggaactcga gaaggacctg ggcttcgaga tcgtcaactt
     3841 ctgctacccg gccggccagt acgacgacga ggtcgtcaag gcagtcgaag ccgccggcta
     3901 ccgcggagcg accaccgtca acccgggcct cgccgaaaag tcaatgccct tcgagctcaa
     3961 ccgaatccga atcgacgccg gcgacggccc cga
//
LOCUS       DAOQKN010000199         3958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_13603_length_3958_cov_4.605176, whole genome shotgun sequence.
ACCESSION   DAOQKN010000199 DAOQKN010000000
VERSION     DAOQKN010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13603_length_3958_cov_4.605176"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..1272
                     /locus_tag="PLJ64_11000"
     CDS             142..1272
                     /locus_tag="PLJ64_11000"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNC94053.1"
                     /translation="MAEELPPLDLARPEVGPEEERLVLEVLRSGRLSLGPVLERFERD
                     FAEWLGVDDAVAVSSGTAGLHLGVRALGWQAGDEVVLSPFTFVASANCLLYEGVKPVF
                     ADIDPVSLNLDPSAAEVAITGATVGLLPIDIFGQPAELPAFERIARSRGLGILEDSCQ
                     ALGAIDSEGRKVGSRGNVSTFAFYANKQMTTGEGGIVIPTDAGMAKVLRSERNQGRAI
                     DMDWLDHDRLGFNYRMSDVTAAIGVAQLGRLDELLAGRARVAGLYLDRLAGVEGLELP
                     APDRPPAKRSWFVFPVRLPEGSDRDGVIRKLGEQGIPAKAYLPSIHLFPHLAELGYRE
                     GQFPVAESVAARSLALPFHAGLSEGDIDRVASALADALGSGA"
     gene            1281..2672
                     /gene="argH"
                     /locus_tag="PLJ64_11005"
     CDS             1281..2672
                     /gene="argH"
                     /locus_tag="PLJ64_11005"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNC94054.1"
                     /translation="MARHTDKSTSRFREPPDPRFWRLNRSIDFDRHLAPYDVAQSRAH
                     AAALNRIGVIADDEIKVIDEGLARIADELNAGEFSFDQGDEDIHMAVERRLGELVGDL
                     AGKLHTGRSRNDQVATDICMAVMDAADGAGQRIATAMNGLLQLAENHRDWTMPGYTHL
                     QRAQPVYLGHHLLSWFWMLSRDFERFAAARRAAAVMPLGAGALAGVNWEIDRRRVAAD
                     LGFESVTVNSLDSTSNRDMVLDYLSAGSICLTHLSRIGSELVIWSSTEFSFCQLSEPF
                     TSGSSIMPQKQNPDSAELLRAKAPRVIGDFTTLLGVLHALPLTYGKDMQEDKEPFFDA
                     VETVEATLEMTAGIFEGIEFDRERLSAAASDEMLAATEIADLLVRRGVPFREAHGIVG
                     DLVRQCVAEGRNLSDLSQDELASRSADLDEEYYEVLQQGSWLESKVSEGGTSSASLAA
                     QIDLAKARLREIG"
     gene            complement(2691..>3958)
                     /locus_tag="PLJ64_11010"
     CDS             complement(2691..>3958)
                     /locus_tag="PLJ64_11010"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNC94055.1"
                     /translation="AATEDAIYSTIGGIGPSASVVIINNKDATVDAMVGGDDYDSTPF
                     NLATQGYRQPGSTIKPFILTTALGQGISPYTTFESAPQVFWRKWKIKDKKTGKWKWHR
                     EPYSVSNYGDSYAGVSDLVSATINSDNSVYAQLGIEAIDGGPKSIAKTIHEMGYSDKI
                     ETNPAMVLGTSEVTPLGWTYAFSTLANDGRRVSGTLAPDPGDDPVSFTKITDEDGKTI
                     KGGDNEVLSKQVIDPDVASEAKTILHEVVTQGTGKNAYIGDDSQWGKTGTTEDNTNAW
                     FCGAITEITACVWVGYPEGYVQMTTEYGGAPVDGGTFPAIIWSRVVSAWKEIEANRAA
                     ERAADAADGDSSDSSDVTTDSSDYSYTAPSTDYTTPTYTAPETTTPVAPAPTPTTTAP
                     APTTTVPTTPTTPSTGGGVAPSSGGASPG"
BASE COUNT          601 a         1270 c         1353 g          734 t
ORIGIN      
        1 cgaagcggga agaaggcggc gattcctggg gctcgatcgg ggtctcgccg aacatcatcg
       61 aggcttcctg ggaagccctg gtcgactcgc ttgcctacgc cttccagaac ggcaactcgg
      121 ccgcggattc cggggaagcc ggtggctgag gaattgcctc cgctcgatct ggcccggccg
      181 gaagttggtc ctgaagagga gcggctggtc cttgaggtcc tgcgctccgg caggctttcg
      241 ctcggtccgg tactggagcg gttcgagcgc gacttcgccg aatggctcgg ggtcgatgac
      301 gcggtggccg tttccagtgg cacggccggc cttcacctcg gggtaagggc tctcggctgg
      361 caggccggcg acgaggtggt gctcagcccg ttcaccttcg tggccagcgc caactgcctg
      421 ctctacgagg gcgtgaagcc ggtctttgcc gatatcgacc cggtttctct caacctggat
      481 ccgtccgccg ccgaggtggc gataaccgga gcgacggtcg gactcttgcc gatcgacatc
      541 ttcggccagc cggccgagct accggcgttc gagcgaatcg cccggtcccg tggcctcggc
      601 attctcgagg actcctgcca ggcgctcgga gcaatcgatt cggaaggccg caaggtgggc
      661 agccgcggca acgtctcgac cttcgctttc tacgcgaaca agcagatgac caccggagag
      721 ggtggcatcg tgatcccgac cgacgccggc atggccaaag tgctgagaag cgagcggaac
      781 cagggccggg cgatcgacat ggactggctg gaccacgacc ggctcggctt caactaccgg
      841 atgtccgatg tgaccgccgc aatcggcgtc gcccagctcg ggcgcctgga tgaactgctg
      901 gccggccgcg cccgggtcgc cgggctctac ctcgaccgcc tggccggggt agagggcctc
      961 gagcttcccg ccccggaccg gccaccggca aagcgcagct ggttcgtgtt cccggttcga
     1021 cttcccgaag ggtccgaccg tgacggggtg atcaggaagc tgggggagca ggggattccc
     1081 gccaaggcgt atctgccctc gattcacctc ttcccgcatc tggccgagct gggttaccgc
     1141 gagggccagt tcccggtcgc cgaatcggtc gcggcccgtt ccctggccct gcctttccac
     1201 gccgggctga gcgagggtga catcgaccgg gttgcctccg cactcgccga tgcgctcggc
     1261 tcaggggcat aatcagcgcc gtggcaagac acaccgacaa gtcgacttca cgcttccgcg
     1321 agccgccgga tccccgtttc tggcggctga accggtcgat cgatttcgac cgtcatctgg
     1381 cgccttacga cgtcgcccag tcccgggccc atgccgcggc cctgaaccgg atcggcgtga
     1441 tcgccgacga cgagatcaag gtgatcgacg agggcctggc ccggatcgcc gatgaactga
     1501 acgccggcga gttcagcttc gaccagggcg acgaagacat tcacatggcg gtcgagcgcc
     1561 gcctaggcga gctggtcggc gacctggccg gcaagctcca caccggccgg tcacgcaacg
     1621 accaggtcgc gaccgacatc tgcatggccg tgatggatgc ggccgacggc gcggggcagc
     1681 ggatcgcgac tgcgatgaac gggctgcttc agctggccga aaatcaccgc gactggacga
     1741 tgcccggtta tacccacctg cagcgggccc agccggtcta tcttggccac cacctcctgt
     1801 cctggttctg gatgctttcg cgcgatttcg agcgcttcgc cgccgcccgc agggctgcgg
     1861 cggtgatgcc gctcggggcc ggcgccctgg ccggggtcaa ctgggagatc gatcgccggc
     1921 gggttgcggc cgacctcggt ttcgagtcgg tcacggtcaa ctcgctggat tcgacctcga
     1981 accgggacat ggtgctggac tacctctcgg ctggctcgat ctgcctgacc cacctttcgc
     2041 ggatcggttc cgaactggtg atctggtcca gcaccgagtt ctccttctgc cagctcagcg
     2101 agcctttcac ctccggctcc agcatcatgc cgcagaagca gaacccggat tctgccgagc
     2161 tgctgcgagc caaggcaccg cgggtgatcg gcgacttcac aacgctgctc ggcgtgctcc
     2221 acgccctgcc tctcacctac ggcaaggaca tgcaggagga caaggagccg ttcttcgacg
     2281 cggtcgagac ggtcgaggcg acgctggaga tgaccgccgg aatcttcgaa gggatcgagt
     2341 tcgatcgcga gcgactttcc gctgccgcct cggacgagat gctcgcggcg accgagatcg
     2401 ccgacctgct ggtgcggcgc ggggttccct tccgtgaggc gcacggcatc gtcggtgacc
     2461 tggtccggca atgtgtcgcc gaaggccgga acctctccga cctcagccag gacgagctgg
     2521 catcccggtc cgccgacctc gacgaggagt actacgaagt tctgcagcag ggcagctggc
     2581 tcgaatcgaa ggtgagcgaa ggtggcacct cgagtgcaag cctcgccgcc cagatcgacc
     2641 tggccaaggc ccggctccgc gaaatcggct gaggccgtgc gctgacccgg ctaaccgggt
     2701 gaagcgccgc cggatgaggg tgcgacgcca ccgcccgtgg aaggcgtggt cggcgtggtg
     2761 ggcaccgtgg tagtcggtgc gggagccgtt gtcgtcggtg tcggggccgg agcgacgggg
     2821 gttgtcgtct ccggggccgt gtaagtcggc gtggtgtagt cggtgctggg tgcggtgtag
     2881 ctgtaatcgc tcgagtcggt ggtcacgtcg gatgaatcgc tgctgtcgcc gtcggcggca
     2941 tccgctgccc gttcggccgc acggttggct tcgatctcct tccaggccga aaccacccgg
     3001 gaccagatga tcgcggggaa ggtgccaccg tcaaccgggg cgccgccgta ctcggtggtc
     3061 atctgtacat agccttcggg gtaacccacc cagacgcagg cggtgatctc ggtgatcgcg
     3121 ccacagaacc atgcgttcgt gttgtcttcg gtcgtgccgg tcttgcccca ctggctgtca
     3181 tcgccgatgt aggcgttctt gccggtgccc tgggtgacaa cctcatgcag gatggtcttg
     3241 gcttcgctgg cgacatccgg gtcgatcacc tgcttgctga gcacctcgtt gtcaccgccc
     3301 ttgatcgtct tgccgtcctc gtcggtgatc ttggtgaagc tgaccgggtc gtctcccggg
     3361 tccggcgcga gggtgccgct gacccggcgg ccgtcattgg ccagggtcga gaaggcatag
     3421 gtccagccga gcggagtcac ttcggacgtg ccgagcacca tcgccgggtt ggtttcgatc
     3481 ttgtcggagt agcccatctc gtggatcgtc ttggcgatcg acttggggcc gccgtcgatc
     3541 gcctcgatgc cgagctgggc gtagaccgag ttgtcggagt tgatggtcgc gctgaccagg
     3601 tcgcttacgc cggcatagga gtcgccgtag ttgctgaccg aatagggctc gcgatgccac
     3661 ttccacttgc cggtcttctt gtccttgatc ttccacttgc gccagaagac ctggggtgcc
     3721 gattcgaagg tcgtgtaggg cgagatgccc tgcccgaggg cggtggtgag gatgaagggc
     3781 ttgatcgtcg agccgggctg cctgtacccc tgggtcgcga ggttgaaggg ggtggagtcg
     3841 taatcgtcgc cgccgaccat cgcgtcaacc gtggcgtcct tgttgttgat gatcacgacc
     3901 gaggcgctcg gaccgattcc gccgatagtg ctgtaaatgg cgtcctcggt tgcggcct
//
LOCUS       DAOQKN010000200         3889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_13967_length_3889_cov_7.595722, whole genome shotgun sequence.
ACCESSION   DAOQKN010000200 DAOQKN010000000
VERSION     DAOQKN010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13967_length_3889_cov_7.595722"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..731)
                     /locus_tag="PLJ64_11015"
     CDS             complement(150..731)
                     /locus_tag="PLJ64_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94056.1"
                     /translation="MVLAFGVAPGTVLASSATDVYTEEPPPPTPGKNDNNGNNTNNGN
                     GGTSGNQNSGANGESGSSGSSGSSGGDSDSSGYSSGYSSGSGDGTSTPSKQYSNGQYN
                     PATDSDESGATGATGSSDDSNPDDSTITATPASGDGGDGGGGSALPLIIGLLIGVPLI
                     AGGGYYAWTRYRVRDEDRDRLKTALSDRKPTGF"
     gene            863..2713
                     /locus_tag="PLJ64_11020"
     CDS             863..2713
                     /locus_tag="PLJ64_11020"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNC94057.1"
                     /translation="MALSGGGYDLLARHLAGILAWVLVAVLLLGPWPDRLRPGRSLAL
                     IGGLLLLLAILSALSSIWSSSVSVSLAEAERVIGYLGFFTASYLTMRTPKQREWFARG
                     IAAGLAVIVLLALGDRLLPGGEPGVVPGIDRLSFPLGYWNANGVCAATALVLFTWFAA
                     VTRSKGWRAGSVAMAMLSGTALYLTYSRGGLLLAVIAAILLCLLTPRRLRVLATLAVV
                     VGGTVPILLTVNLYPEISGDSFGDPSLGESLIVILVALAASLIAAAVLEGLFGLGRRQ
                     PEVTERAREISRDRRVLIGIIGAGATLLIVFSFAFGQNVWDQFTDDDVPVPTDARSRF
                     TELSGSWRYEFNRVALDTFSDNPVLGTGAGTYPIEWSRRREVPVITQDAHSFYLENLA
                     ELGIPGGLISISLVISLLTLAAWAFRRRLGREAPVILALTAGLLFSLAFDWFWKLGVT
                     AALLMCLAAWIASAEAVDPERPQTGAGTGFKVAGLLAAWVSIVILAVPALADHYVEAS
                     ADSVRAGNVDRAYDQAKAASRLEPFSADPHMQLGTIAEGRGQYALALAEYNKAIELEP
                     ENWHALVLRFGLNYHAGKLQAARADYARLREINPLYFSEYSFREIQDMAS"
     gene            2847..>3889
                     /locus_tag="PLJ64_11025"
     CDS             2847..>3889
                     /locus_tag="PLJ64_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94058.1"
                     /translation="MLLAALLISFLGLTTFVSKADAARSFQTGIFDGSVYDHGDDEAF
                     DRTADSGATLIRSNLYWTYMVANGDSADRPGSEGQPFNATDPGSPYYNWGVYDRMVRK
                     SAARGLQMVFSIVSPPRWARISSCADSGVCSPRPADYADFATAAARRYDGTFDPGDGN
                     GVLPRVRFWQAANEPNFYLFYKPLLKPNGAPAAPYEYRKILNAFYGAIHAVDNSNVVI
                     AAGLAPNGKKGKSISPLDFTRRALCMTGSYKHPRPIAGCKFKVKADAWAVHPYTTGGP
                     THVPANGNDMSVAALPRLQKLLKAATRAGRLSGAGGRTQLWVTEFSWDSKAPDPGGVP
                     WALQTRWVAQAMYL"
BASE COUNT          580 a         1351 c         1213 g          745 t
ORIGIN      
        1 acggcaggag atcgcctttc atcgcggcat gggccgtttt taccggcgct tcgacgcgcc
       61 ggagaacccg ggcgtggtca atctcgcggt ctatctgggg atcggcgcga agctggcggt
      121 cagcctgctg atcacggctg ccggctgctt cagaaaccgg tcggcttgcg gtcgctgagg
      181 gcggtcttga ggcggtcccg gtcctcgtca cgcacccggt agcgggtcca ggcgtagtag
      241 ccgccgccgg cgatcagcgg tacgccgatc aggaggccga tgatcagggg cagggccgaa
      301 ccgccgccgc cgtcgccgcc atcccccgag gccggggtcg cggtgatcgt cgaatcatcc
      361 gggttcgaat cgtcgctcga accggtcgct ccggtggcac ccgactcgtc ggaatccgtg
      421 gccgggttgt attgcccgtt gctgtactgc ttgctcgggg tcgaggtgcc atcccccgat
      481 ccggagctgt aaccggagct gtagccgctc gaatccgaat ctccgccgct gctgccactg
      541 ctgccgctgc tgccggattc accatttgcg ccgctgttct ggttgccgct tgtgccgccg
      601 ttgccgttgt tggtgttgtt cccgttgttg tcgttcttgc cgggggtggg cggtggcggc
      661 tcttccgtgt agacgtccgt agctgacgaa gcaagtaccg tccccggtgc cacaccgaat
      721 gccaggacca tcagccccaa gatcgatgcc gcaaggcaga gcaggcgccg cgcggaagcg
      781 gaacttgtcg caggagcgtt catacgcacg gaagatagcc gatctgatcg cgtttctggt
      841 tccattcctg ctcctcgtcg gtttggcact gagtggcggc ggttatgacc ttctggctcg
      901 tcacctggct ggaatcctcg cctgggtcct cgtcgccgtg ctgctgctcg gaccctggcc
      961 ggaccggctc cgcccgggcc ggtcgctcgc cctgattggc ggtctgcttc ttctgctggc
     1021 aattctttca gcgctttcgt cgatctggtc gagttcggtt tcggtcagcc tggccgaagc
     1081 cgagagagtc attggctatc tcggtttctt tacagccagt tacctcacca tgagaacccc
     1141 gaaacagcgc gaatggttcg cccggggcat cgcggccggc ctggcggtga tcgtcctgct
     1201 ggcgctcggc gaccggttgc tgcccggagg ggagcccgga gtcgttcccg gaatcgaccg
     1261 tctcagcttc ccgctcggct actggaacgc gaacggcgtc tgcgccgcca cggcccttgt
     1321 cctcttcacc tggtttgccg cggtgaccag gtcgaaaggg tggcgggccg gatccgtggc
     1381 catggccatg ctctccggaa ccgcgctcta tctcacctac tcccggggcg gcctgctgct
     1441 cgccgtgatc gcggccatcc tgctttgcct gctcaccccg cgccggctcc gggtcctggc
     1501 caccctggcg gtcgtggtcg gcggaacggt cccgatcctg ctcacggtca atctctatcc
     1561 ggagatctcc ggcgacagct tcggtgaccc ctccctcgga gagagcctga tcgtgatcct
     1621 tgtcgccctc gcggcctcac tgatcgcggc ggcggtcctc gaaggcctgt tcgggctcgg
     1681 ccgccgtcag ccggaggtga cagagcgagc ccgcgagatt tcccgggatc gaagagtgct
     1741 catcgggatc atcggggccg gagccaccct gctgatcgtc ttttcgttcg cgttcggcca
     1801 gaacgtctgg gatcagttca ccgacgatga cgttcccgtc ccgaccgatg cccgctcgcg
     1861 cttcacggaa ctttcgggat cctggcgata cgagttcaac cgggttgccc tggacacctt
     1921 ctccgacaac ccggtcctcg gaaccggtgc cggcacctac ccgatcgaat ggtcccgccg
     1981 ccgcgaggtg ccggtgatca cccaggacgc ccactccttc tatctggaga acctggccga
     2041 gctcggcatc ccgggcgggc tgatctcgat ctccctggtc atttcacttc tgaccctggc
     2101 ggcgtgggcc tttcgccggc gtctgggaag ggaagctccg gtgatcctcg ccctgaccgc
     2161 cggactgctc ttctccctgg ccttcgactg gttctggaaa ctcggggtca ccgcggccct
     2221 gctgatgtgc ctcgccgcct ggatcgcctc ggccgaggcg gtggacccgg agcgcccgca
     2281 gaccggtgcc ggtaccggct tcaaggtcgc cggcctgctc gccgcgtggg tctcgatcgt
     2341 gatcctggcc gttccggcac tggccgacca ctacgttgag gcaagcgccg attcggtccg
     2401 ggcaggcaac gtcgaccgcg catacgacca ggcgaaagcc gcctcaaggc tggaaccctt
     2461 ctcggccgac ccccacatgc agctgggcac gatcgccgaa gggcggggcc agtatgccct
     2521 ggcgcttgcc gagtacaaca aggcgatcga acttgagccg gagaactggc atgccctggt
     2581 cctgcggttc ggactcaact accacgcagg gaagctgcag gcggcccggg ccgactacgc
     2641 gcgcctgcga gaaatcaatc cgctctactt ctccgagtac tcattcaggg aaatccagga
     2701 catggcctcc tgacccccaa ctcgccggtt cgctactttc tgcctttggc gatgttcaag
     2761 tacacaagcg ccacccgaga cttcgcgccc tcgagcgaag tttgaccctc cgaaaggatc
     2821 ccttgcacgc aatctcgaat cgcggaatgc tgctcgcagc actgctgatc agctttctcg
     2881 gcctgaccac ctttgtctca aaggccgacg cagcgcgctc tttccagacc gggatctttg
     2941 atggcagcgt gtatgaccac ggtgacgacg aggccttcga tcgcaccgcc gacagcggcg
     3001 caacgctgat caggagcaac ctttactgga cctacatggt cgccaacggc gattcggccg
     3061 atcgcccggg atccgagggc cagcctttca acgcgaccga ccccggcagc ccctactaca
     3121 actggggggt ctatgaccgc atggttcgca aatctgccgc ccgcgggctc cagatggtct
     3181 tctcgatcgt cagccctccg cgctgggcac ggatttcgtc ctgtgccgat tccggtgtct
     3241 gctcgccccg gcccgccgat tacgcggatt tcgcgaccgc ggctgccagg cgctacgacg
     3301 gaaccttcga ccccggtgac ggcaacggtg tcctgccccg cgtgaggttc tggcaggccg
     3361 ccaacgagcc caacttctac ctcttctaca agccgctgct gaagcccaac ggggcaccgg
     3421 ctgctcccta cgagtaccgc aagatcctca acgccttcta cggcgcgatc cacgcggttg
     3481 acaacagcaa cgtcgtgatc gctgccgggc tcgccccgaa cggcaagaag ggcaagtcga
     3541 tctcgccgct cgatttcacc cgtcgcgccc tctgcatgac cggctcctac aagcatcccc
     3601 gcccgatcgc cggctgcaag ttcaaggtga aggccgacgc ctgggcggtt cacccgtaca
     3661 cgaccggcgg tccgacccac gttcccgcca acggcaacga catgtcggtt gccgccctgc
     3721 cccgcctgca gaagctgctc aaggcggcga ccagggccgg caggctctcc ggcgcgggcg
     3781 gccgcacgca gctgtgggtc accgagttca gctgggattc aaaggcgccg gacccgggcg
     3841 gagtgccgtg ggccctgcag acccgctggg tggcgcaggc catgtacct
//
LOCUS       DAOQKN010000201         3850 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_14219_length_3850_cov_5.549934, whole genome shotgun sequence.
ACCESSION   DAOQKN010000201 DAOQKN010000000
VERSION     DAOQKN010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14219_length_3850_cov_5.549934"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="PLJ64_11030"
     CDS             <1..928
                     /locus_tag="PLJ64_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94059.1"
                     /translation="TGVFPLREVTTSFLWAVPGLIVCAGFAFVLGRRRQIPPEFLLAA
                     GAALVFWVLACLNFIPGRDFVASRYQYPSVIFLLMMLAGACSGLRPGRQLFRGLAALT
                     VVAVAINAAALIYIFNHTYKVYEGRNLSGMTALDISRGTVVPGFGIAIGTDGAGAHID
                     ADTYFNAVDRYGSPGWDKEEIEAASGEDRSRIDTILVGALRVRLIPAKRVSPLPRTCR
                     IAEATEAIGDPVTVTSPVLLIEPEKTVGIYLGRFGPDASAPAWGAVGGVPTGYLIPSG
                     RSDRPWRIGFKGEGKVRVCDARLAPETGDPEK"
     gene            938..2608
                     /locus_tag="PLJ64_11035"
     CDS             938..2608
                     /locus_tag="PLJ64_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94060.1"
                     /translation="MGAASSGMPEERPDRIWIWLLGLAMLASGVLLMVMRSQIGFFLD
                     DWDLVIYRGNPTDWLLPHNEHIVVLPAAIYKLSLAIFGMTAIPLHLVAVALFLTSVAA
                     LFLWLRPLTGAPSAFFGCLVVLFLGAATEDLVWAFQMGFFGSVAAGLGALLQLRRGTR
                     TGDRWACLLLVVSVLFSSLGIPFTLGAAVLILFGESLHPDWRRFPGRAWIFLVPALLY
                     AGWWLGWGHLAPNALGTANLRQDPVYLLSGLGYSALVLTGTFKVGQVSMDDGWALPGL
                     ILALILGLVLFIRRRIPVELLVAATIALGFWALSGLNYIPGREFFNSRYQYPGAVILL
                     MLLGGAFAGSRPGPRTLAVIAAVCAVSLVVNLQTLNDLFRGTYRPYAQRNHLALAGLD
                     LASRTVPDGYAVAMTDDGQALVDAGSYLEAAEKYGKAGLPPDEIGGLPVDQRIRLDQI
                     LVGALPVRLLPAGNLAADRDLCRTVTADPAASGNLEVGSSLMLIRSRRQVTIKLGRFG
                     PGTGALAWFAPGGRAVGYRIPRDHSERPWRIGFKGRGPVTVCPASPSK"
     gene            2618..>3850
                     /locus_tag="PLJ64_11040"
     CDS             2618..>3850
                     /locus_tag="PLJ64_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94061.1"
                     /translation="MGAGPKSFQAKVREQPWPWLLGLLTLLSAALLMTLRADLRFFLD
                     DWALVIYREGGPTDWLLPHNEHIIVLPNALYKLSLTVFGMTTIPIHVAGLALFLASVW
                     LLFFWLRPLVGEPASAIGCAVLLFLGASGEDLVWTFQIGYLGSVAAGLAALLLLRRGS
                     TRADAWACASLVVCLLFSSLVIPFLAGVAVLILFPRDLSPDWSALKRRCWVFLVPAVL
                     YAIWWLGWGHLAENAISLHNAAHLPAYVPAAFAFAASTLTGIFLIHLPHDQSGWAIPG
                     LVLAGGVAFVAWRRRRIAPELLTAAAIALVFWALSGLNFIPGREFDASRYVFPGAIFL
                     LMILGGAFAGCRPGPRTLAVIAAVAAVAIATNLDALFHKYDDQIRPIGERNAASLTAI
                     DLARDTVDPGYSVGMTDDG"
BASE COUNT          487 a         1358 c         1267 g          738 t
ORIGIN      
        1 gaccggcgtg ttcccgctgc gggaggtgac cacgagcttt ctctgggccg ttcccgggct
       61 gatcgtctgt gccggattcg cctttgtcct cggtcgccgc cggcagatcc cgcccgagtt
      121 tctgctcgcg gccggcgcgg ccctcgtctt ctgggtgctg gcctgcctca atttcattcc
      181 gggccgggac ttcgtggcca gccggtacca gtacccgagc gtgatcttcc tgctgatgat
      241 gctggccgga gcctgctccg gcctgcgccc gggccgacag ctcttccggg ggcttgccgc
      301 gttgaccgtg gtcgcagtgg cgatcaacgc ggctgccctg atctacattt tcaatcacac
      361 ctacaaggtc tatgagggcc gcaacctgag cggaatgacc gcgctggaca tctcccgggg
      421 aacggtcgtt cccggcttcg gaatcgcgat cggaaccgat ggtgccggtg cccacatcga
      481 cgccgatacc tacttcaacg cggtcgaccg ctacggctcg cccggctggg ataaagagga
      541 gatcgaggcg gcttcgggag aggatcgaag ccggatcgac acgatcctgg tcggcgccct
      601 gcgggtccgg ctgattcccg caaaacgggt atccccgctt ccccggacct gccggatcgc
      661 cgaggcgacg gaggcgatcg gtgatccggt caccgtcacc tctccggtgc tcctgatcga
      721 gccggagaag acggtcggca tctaccttgg ccgcttcggg ccggacgcat cggcaccggc
      781 ctggggggcg gtaggtggcg tcccgaccgg ttacctgatc ccttccggcc gctccgaccg
      841 gccctggcgg atcggcttca agggagaagg caaggtcagg gtctgcgacg cccggctcgc
      901 ccccgaaacc ggcgatccgg aaaagtaacc tgcccccatg ggtgcggcgt cgagcggaat
      961 gcccgaagaa agaccggacc ggatctggat ctggctgctt ggcctggcga tgctggcctc
     1021 gggcgtcctg ctgatggtga tgcggtcgca gatcggcttc ttcctggatg actgggacct
     1081 ggtcatctac cgtggcaacc cgaccgactg gctgttgccc cacaacgagc acatcgtggt
     1141 gttgccggcc gcgatctaca agctgtcact ggcgatcttc gggatgaccg cgatcccgct
     1201 ccacctggtg gccgtcgcac tgtttctgac ctcggtggcg gcgctgttcc tctggcttcg
     1261 ccccctgacc ggcgcgccct ctgccttctt cggctgcctg gtcgtcctct tcctcggagc
     1321 ggcgaccgaa gacctggtct gggctttcca gatgggcttc ttcggctcgg tcgccgccgg
     1381 tctcggggcg ctgctccagc tgcggcgggg gacccggacg ggggaccggt gggcctgcct
     1441 gctgctggtg gtctcggttc tcttctcctc gctcggcatc cccttcacgc tcggcgcggc
     1501 cgtcctgatt ctgtttggcg agagccttca cccggactgg aggcggttcc ccggccgtgc
     1561 ctggatcttc ctggtgccgg ccctgctcta cgccggctgg tggctgggct gggggcatct
     1621 ggccccgaac gcgctgggca cggcgaacct ccggcaggac ccggtctacc tgctgtcggg
     1681 tctcggctac tcggccctgg tcctgaccgg caccttcaag gtcggccagg tttcgatgga
     1741 cgacggctgg gcgctgccgg ggctgatcct cgccctgatt ctgggactgg tgctcttcat
     1801 ccggcgccgg atcccggtgg agctgctggt cgcggccacg atcgcgctcg gcttctgggc
     1861 cctctccggt ctcaactaca tcccgggccg cgagttcttc aacagccgct accagtaccc
     1921 gggagcggtg atcctgctga tgctgctcgg cggcgccttc gccgggtcgc ggcccgggcc
     1981 gcggaccctg gccgtgatcg cggcggtctg tgccgtctcg ctggtggtga atctccagac
     2041 cctgaacgac ctcttccgcg gcacctaccg accctacgcc caacgcaatc acctcgccct
     2101 tgccggtctc gacctcgcca gtcgcacggt gccggacggg tacgcggttg cgatgaccga
     2161 cgacggccag gccctggtcg atgccgggag ctacctggaa gcggcggaga agtacgggaa
     2221 agccgggctg ccacctgacg aaatcggggg cctgccggtc gatcagcgaa tccggctcga
     2281 tcagatcctg gtcggcgccc tgccggtccg gctcctgccc gccggaaatc tggcggccga
     2341 ccgcgacctg tgccggaccg tcaccgccga cccggccgcc agcgggaacc tcgaagtcgg
     2401 ctcctccctg atgctgatcc gctcccggcg gcaggtgacg atcaagctgg gccgcttcgg
     2461 cccggggacc ggagcattgg cctggtttgc tcccggaggg cgagcggtcg gctaccggat
     2521 acctcgcgac cactcggaaa gaccgtggcg gatcggcttc aagggaaggg gcccggtgac
     2581 cgtgtgtccc gcttcaccct caaagtaggc tccgcgaatg ggagcgggcc caaagagttt
     2641 ccaggcgaag gtccgcgagc agccgtggcc gtggttgctc ggtctgctca cccttctttc
     2701 cgccgcgctg ctgatgaccc tgcgggcgga cctgcggttc ttcctcgacg actgggccct
     2761 ggtcatctac cgcgagggcg ggccgaccga ctggctcctc ccccacaacg agcacatcat
     2821 cgtgctcccg aacgcgctct acaagctctc gctcaccgtg ttcgggatga ccacgatccc
     2881 gatccacgtg gccgggctgg ccctctttct ggccagcgtc tggctgctct tcttctggct
     2941 caggccgctg gtcggggaac cggcctcggc gatcggctgc gccgtcctgc tcttcctcgg
     3001 agcctccggc gaggatctcg tctggacctt ccagatcggc tacctcgggt cggttgcggc
     3061 cgggctggcg gcgctgcttc tgctccgccg cggatcgacc cgcgccgatg catgggcctg
     3121 cgcctcgctg gtcgtctgcc tgctgttttc gtctctggtc attcccttcc tggccggggt
     3181 cgcggtcctg atcctcttcc cgcgggacct cagcccggac tggtccgccc tgaaacgccg
     3241 ctgctgggtc ttcctggtgc cggctgtcct ttacgcgatc tggtggctgg gctggggtca
     3301 cctcgccgag aacgcgatca gcctgcacaa cgcggcgcac ctgccggcct atgtgccggc
     3361 agcattcgcc ttcgcggcca gcaccttgac cggaatcttt ttgattcacc ttccccatga
     3421 ccagtccgga tgggcgatcc ccggtctggt cctcgccggc ggagtcgcct tcgtggcctg
     3481 gcgacgccgg cgaatcgccc cggagctcct gaccgcagcc gcgatcgcac tcgtcttctg
     3541 ggcgctctcg ggtctgaact tcataccggg gcgcgaattc gacgccagcc gctacgtgtt
     3601 cccgggggcg atctttttgc tgatgatcct cggcggcgcc ttcgccggtt gccgtccggg
     3661 cccccggacg ctcgccgtga tcgcggcggt ggcggcggtt gccatcgcga ccaacctcga
     3721 cgcgctcttc cacaaatacg acgaccagat ccggccgatc ggggagcgga acgcggccag
     3781 cctgaccgcg atcgacctgg cccgtgacac cgtcgacccc ggctactcgg tcggaatgac
     3841 cgacgacggg
//
LOCUS       DAOQKN010000202         3838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_14291_length_3838_cov_6.471319, whole genome shotgun sequence.
ACCESSION   DAOQKN010000202 DAOQKN010000000
VERSION     DAOQKN010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14291_length_3838_cov_6.471319"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..480
                     /locus_tag="PLJ64_11045"
     CDS             157..480
                     /locus_tag="PLJ64_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HNC94062.1"
                     /translation="MSTETTKIPVCPVSELEPGDRRIIEHEGEFLAVVNCDGELFGIE
                     DRCSHDDGPLAEGEINQAECTVECPRHGSLFDLRTGKPKTLPAYQPVKTFPVEVIDDT
                     ITLEV"
     gene            483..1916
                     /gene="sufB"
                     /locus_tag="PLJ64_11050"
     CDS             483..1916
                     /gene="sufB"
                     /locus_tag="PLJ64_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HNC94063.1"
                     /translation="MATAEVLAEKEKVQGINADYAEKFGFSDPETGYAYKAPKGLTRE
                     IVEQISEYKDEPQWMRDFRLKALEIFESKPTPQWGADLDQIDFDDIHYFVRASEKNSR
                     DWSEVPEDIKNTFDRLGIPEAEQKFLSGVGAQYESEVVYHQVNEKLEAQGVIFTDMDT
                     ALREHEDIIKEYWATVIPANDNKLAALNSAVWSGGSFVYVPKGVKVEMPLQAYFRINT
                     ENMGQFERTLIIAEEDSDVHYIEGCTAPVYSTDSLHSAVVEIIAKPGARVRYTTVQNW
                     SQNVFNLVTKRAIAEERATMEWVDCNLGSKVTMKYPAIYLLGEGAHGEVLSIAFAGEG
                     QHQDAGAKIIHGAPNTTSSVFSKSISKDGGRSTYRGLLEVADGATGTRSKVVCDALLL
                     DEKSQSDTYPTIRIGESDADMGHEATVSKVGDDMLFYLQSRGLSEEEASKMIVNGFIE
                     PVTKELPMEYAVELNRLIELQMEGSIG"
     gene            1909..3081
                     /gene="sufD"
                     /locus_tag="PLJ64_11055"
     CDS             1909..3081
                     /gene="sufD"
                     /locus_tag="PLJ64_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HNC94064.1"
                     /translation="MASAELAVDAPAWLVERREKGAALVETIELPHAKTKGWEFTDIS
                     RLDLDSYENSPAEVTISGGSDEIIVLPLSEALASHSDLLAEKLGSLVSIEDPFVARNE
                     ATLNDGVLVYVPKNVECEEAIKVDLKLDTDGAAVNWRTLVVLEEGAKAEVWEHYGSAS
                     DETDALLNTVVELVVGQAANLHFVATQDLSEKAWLFSTQRGEVQRDGSLDWATLGFGG
                     GGGKVRMTTNLAGPGSDAKVTGGYAGGNSQHIDYDTLQEHAAEHTTSDLAFRGVLADK
                     SSAVWRGMIKVDEGAQQTDAFQECRNMLLSTDAHADAIPGLEILADDVACTHAAAIAQ
                     VDKDQLFYLESRGLSEETSRSLVVEGFLQSLVERLGEGPVRDEIAERLETRLAEIL"
     gene            3108..3722
                     /locus_tag="PLJ64_11060"
     CDS             3108..3722
                     /locus_tag="PLJ64_11060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNC94065.1"
                     /translation="MFDMDGVLIDTQAAVHDAYTAWAGENGLDPQEVLAIIHGRRTVE
                     VAQIFDVDEDVEAEADRIEKAIAERARAEDGIEPVCELYRSLDRGRVAVATSARLETA
                     LTNLRVLGLEHPEVLVTGQDVESGKPSPDPYLLAAERLGVEPAECVVVEDAPAGIKAG
                     KAAGTFVVALTTTHEAIDLGEADRVIAPGQLEDLFTSAEPSSGA"
BASE COUNT          746 a         1274 c         1194 g          624 t
ORIGIN      
        1 ccgcttgggg agtgcgttag gggtgcctaa tggggggtgc cggtggggtt gagtcggtaa
       61 gatctaattc ctagccgcat tgtcggctta ttgtccgggt ttatccgcag ctggccttct
      121 gccgagcggc ttgccggacc ccctcccaat caccttatga gcacagaaac cacaaagatt
      181 cccgtctgcc cggtctccga acttgagccc ggtgaccgcc gcatcatcga acacgaaggc
      241 gagtttctcg cggtcgtgaa ctgcgatggc gagctgttcg ggatcgaaga ccgttgctcg
      301 catgacgacg gtccgctggc ggagggcgag atcaaccagg ccgaatgcac cgtggaatgc
      361 ccgcggcacg gctccctctt cgacttgcgg acaggcaagc cgaagaccct gccggcctac
      421 cagccggtca agacttttcc tgtcgaggta atcgacgaca ccatcacgct ggaggtttga
      481 acgtggctac tgccgaagta ctcgccgaaa aagagaaggt ccaggggatc aacgctgact
      541 acgccgagaa gttcggcttc agcgatcccg aaaccggcta cgcctacaag gccccgaagg
      601 gcctgacccg ggagatcgtc gagcagatct ccgagtacaa ggacgagccg cagtggatgc
      661 gggacttccg gctcaaggcg ctggagatct tcgagagcaa gccgaccccg cagtggggcg
      721 ccgacctcga ccagatcgac ttcgatgaca tccattactt cgtccgcgcc tccgagaaga
      781 acagccgcga ctggtccgag gtccccgagg acatcaagaa cactttcgac cgcctgggca
      841 tccccgaagc cgagcagaag ttcctctccg gcgtcggtgc gcagtacgaa tccgaggtcg
      901 tctaccacca ggtgaacgag aagctcgaag cgcagggtgt gatcttcacc gacatggaca
      961 ccgcgctccg cgagcacgag gacatcatca aggagtactg ggcgaccgtg atcccggcca
     1021 acgacaacaa gctggccgcg ctcaactctg cggtctggtc cggcggctcc tttgtctacg
     1081 tgccgaaggg cgtgaaggtc gagatgccgc tccaggccta cttccggatc aacaccgaga
     1141 acatgggcca gttcgagcgg accctgatca tcgccgaaga agattccgac gtccactaca
     1201 tcgagggctg caccgccccg gtctactcga ccgactcgct tcactccgcg gtggtcgaga
     1261 tcatcgccaa gcccggtgcc cgggtccgct acacgaccgt ccagaactgg tcgcagaacg
     1321 tcttcaacct ggtcaccaag cgggcgatcg ccgaggagcg ggcgaccatg gaatgggtcg
     1381 actgcaacct cggttcgaaa gtcaccatga agtacccggc gatctacctg ctcggcgaag
     1441 gcgcccacgg cgaagtgctg tcgatcgcct tcgcgggcga aggccagcat caggacgccg
     1501 gcgcgaagat catccacggt gccccgaaca cgacctcgtc ggtcttctcg aagtcgattt
     1561 ccaaggacgg cggccgttcc acctaccgcg gactgctcga ggtcgccgat ggtgcgaccg
     1621 gcacccgctc gaaggtggtc tgcgacgcgc tgctgctcga cgagaagagc cagtcggaca
     1681 cctacccgac gatccggatc ggcgagtccg atgccgacat gggtcacgag gccaccgttt
     1741 ccaaggtcgg cgacgacatg ctcttctacc tgcagagtcg cgggctctcc gaggaagaag
     1801 cttcgaagat gatcgtcaac gggttcatcg aaccggtcac caaggaactg ccgatggagt
     1861 acgcggtcga actgaaccgg ctgatcgagc tgcagatgga gggttcaatt ggctagcgcc
     1921 gagctggcag ttgacgcacc cgcctggctc gtcgagcggc gggaaaaggg cgctgccctg
     1981 gtcgagacga tcgaactccc ccacgccaag accaagggct gggagttcac cgacatctcg
     2041 cggctcgatc tcgactccta cgagaactcg ccggccgagg tgacgatcag cggtggcagc
     2101 gacgaaatca tcgtcctgcc gctgagcgag gcgttggcct cccactccga tctgctcgcc
     2161 gaaaagcttg gttcgctggt ctcgatcgag gaccccttcg tggcccgcaa cgaggccacc
     2221 ctgaatgacg gcgtgctcgt ctacgtgccg aaaaacgttg agtgcgagga agcgatcaag
     2281 gtcgatctga agctcgacac cgacggcgct gcggtgaact ggcgcaccct ggtcgtgctc
     2341 gaggaaggcg ccaaggccga ggtctgggag cactacggct ccgcctccga cgagaccgac
     2401 gcactgctca acaccgtggt cgaactggtc gtcggccagg ccgccaacct tcacttcgtc
     2461 gcgacacagg acctttccga gaaggcctgg ctcttctcga cccagcgggg cgaggtccag
     2521 cgggacggct cgctcgactg ggcaaccctc ggtttcggcg gtggcggcgg caaggtccgc
     2581 atgaccacca acctggccgg gcctggctcc gatgccaagg tcactggcgg atacgccggc
     2641 ggcaactcgc agcacatcga ctacgacacg ctgcaggagc atgcggccga acacaccacc
     2701 tcggacctgg ccttccgcgg cgttctggcc gacaagtcct cggctgtctg gcgcggcatg
     2761 atcaaggtcg acgaaggcgc ccagcagacc gacgccttcc aggagtgccg caacatgctg
     2821 ctctccaccg acgctcacgc ggacgcgatc cccgggctcg agatcctcgc cgacgacgtc
     2881 gcctgcaccc atgcggcggc gatcgcccag gtcgacaagg accagctctt ctacctggaa
     2941 agccgcggcc tcagcgagga aacctcccgc agcctggtgg ttgaaggctt cctgcagtcg
     3001 ctggtcgagc ggctcggcga aggccccgtc cgggacgaga tcgccgagcg gctggaaacc
     3061 cgcctggccg agatcctctg aagctgccgc ctgaaatcac caccgtcatc ttcgacatgg
     3121 acggggtgct gatcgacacc caggcggccg ttcacgacgc atacaccgcc tgggccgggg
     3181 agaacgggct cgacccgcaa gaagtcctcg ccatcatcca cggccgccgt accgtcgagg
     3241 tcgcgcagat ctttgacgtg gacgaagacg tcgaagcgga agcggaccga atcgagaagg
     3301 cgatcgccga gcgggcccgg gccgaagacg ggatcgaacc ggtttgcgag ctctaccgct
     3361 cccttgaccg cggccgggtt gccgtcgcca cctcggcccg tctcgaaact gcgctcacca
     3421 acctgcgggt gctcggactc gagcatcccg aggttctggt caccggccag gacgtcgaat
     3481 ccggcaagcc ctccccggac ccctacctgc tcgctgccga gcggctgggc gtcgagccgg
     3541 ccgaatgcgt cgtggtcgag gacgcgccgg ccggaatcaa ggcgggaaag gccgccggca
     3601 ccttcgtggt cgcattgacc accacccacg aagcgattga cttgggcgaa gccgaccggg
     3661 tgatcgcccc cgggcaactc gaagacctgt tcacatcggc tgagccttca agcggcgcct
     3721 gaagcgctct acttgacctt gccggtccgt ttgaggttga tcttcagcac cgactccccg
     3781 gcggtgctgg tactgaccac ctgaaccgca aggctcttca accccttctt gcccttct
//
LOCUS       DAOQKN010000203         3838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_14293_length_3838_cov_5.447793, whole genome shotgun sequence.
ACCESSION   DAOQKN010000203 DAOQKN010000000
VERSION     DAOQKN010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14293_length_3838_cov_5.447793"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            589..2055
                     /locus_tag="PLJ64_11065"
     CDS             589..2055
                     /locus_tag="PLJ64_11065"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNC94066.1"
                     /translation="MGYETRCLIGGEFVEGEGASIEVENPASEEIVATVGSASLEQLD
                     LAVAKATEAKASWGGMPAVDRAPLLHGIAAALREKTDELALLMTTEIGRPLSESADEV
                     EWCAACFDYYAEVARSEIGRVIPPIEASQFSVVVKEPHGVVGCIVPWNFPLLLLVWKV
                     APALAAGNTIVAKPSELTPLSTLAIAEVFSELPDGTVNLLAGAGDVGAGISGHPGIDC
                     VAFTGSVATGRKVGVAAASRNARVNLEMGGKDPFIVCDDIGTDGLEVAARGGAWAAFL
                     NAGQVCTSSERFYVMESVFDEYVSAFVEHTRSLVIGDPLESATDLGPMVSATQRGKAL
                     GQVEDAVAGGAELLTGGGNAGFGKGHYLEPCVLTGVDAAADLLREETFGPVAPIVPVK
                     DLDEAIALANGLDFGLGANIYTRDLKRAIRCVKEVRAGSVWINDPLTDNDAGPFGGFK
                     NSGIGRELGTEGLDAFREAKHAHIDTEIEVKDWWYPYG"
     gene            complement(2084..2653)
                     /locus_tag="PLJ64_11070"
     CDS             complement(2084..2653)
                     /locus_tag="PLJ64_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94067.1"
                     /translation="MNLPSDTTRASRFIPAAFLCALSIALLAGCGGGSSEEDLSREGF
                     IADADAICAQYQPTSATLEQQFNQAIGNGELEDAAQDFEDQAADFSAMLDELEGLTAP
                     TADQTTVTQIIDLGRQRVDIAKEAADAIASGDKDAMISAGKKASILAGQYYQLTDGFG
                     FETCGSSGASGTTGTTGPAGSTGTTGATS"
     gene            2705..>3838
                     /locus_tag="PLJ64_11075"
     CDS             2705..>3838
                     /locus_tag="PLJ64_11075"
                     /inference="COORDINATES: protein motif:HMM:NF013444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HNC94068.1"
                     /translation="MSNGQDRTPYVDALLAFAQSDPGRFNVPGHQGGIGADPLLHQLV
                     GQTGLSDDIPGLIEGIDVGDPNPFQEAQALAAGAWGAKRTWFLINGASQGNQAMCLAV
                     AHMGGELVVQRNVHSSVIDGMIMAGLKPTFAAPELDPDLGVAHCLTPDDLAEALDRCP
                     DASAAVVVSPTYFGACADISGLAEVAHSRGVPLVADEAWGAHLHFHPDLPPSAIESGA
                     DLVISSTHKIVGSLTQSAMIHLATDRFDQAIIDRCISMTESTSPNSLLCGSLDAARRQ
                     AAIHGERLLGETIEVLVEAREELRSIPGLDVLDERLLERKSVVGWDPLRLTIDVRGTG
                     STGYHIAELARDLAGVNLELASDTVAVAVFGMGTGTAQRARRLI"
BASE COUNT          622 a         1291 c         1274 g          651 t
ORIGIN      
        1 ccgggacgga cgcacgcgcg gcattcagcc gcgctggtcc tggagtgtcc cggacaaccc
       61 ccacccgacc ccacggctac acggacccgg agaaacctcg acggccccac tcgaccccac
      121 ggctacacga acccgggaga aacctcaatg acctccatcc gaccccacgg ctactcggac
      181 cccgtgctgg tcgacgggat tgccctggac ctccggaggc gattcgcgag agtccacttt
      241 tggccgcatg gcgcactcaa ctccgatctc gaatcggcgg cccggatcga aatcggagtt
      301 gggtgcgcta tttgccgata actcccgcct cgaatccccc ccgagattcc aacccgctcc
      361 cccttcacca gcccctgccg gcttcaaggt gacgcaggtt tgcgacctct ccggtggcaa
      421 acctgcggca ccttcgaaaa acacacccgg cacgtctcga ggtgccgcgc attcaggacc
      481 gttacgggct taaacgtgcg gcacctgcga gcaacccagg tgttgcaggt ccggcgggtt
      541 gaagctggaa gcggggcgaa gcttgccggc tgcagttagt tttcccgcat gggatatgag
      601 actcgttgtc tgattggtgg agagttcgtt gagggagaag gcgcctcgat cgaggttgag
      661 aacccggctt ccgaggagat cgtcgcgacc gttggttcgg cttcgctgga gcagcttgac
      721 ctcgccgtcg ccaaggcgac cgaggcgaag gccagctggg gcgggatgcc ggccgttgac
      781 cgggctcctc tgctgcacgg gatcgccgcg gccctgcgcg agaagaccga cgaactcgcg
      841 ctgctgatga ccaccgagat cggccggccg ctttccgagt cggcggacga ggtcgaatgg
      901 tgcgcggcct gcttcgacta ctacgcggag gtcgcccgtt cggagatcgg acgcgtgatc
      961 ccgccgatcg aggcctcgca gttctcggtc gtggtcaagg agccacacgg ggtggtcggc
     1021 tgcatcgtgc cgtggaactt ccccctcctc ttgctggtct ggaaggtggc cccggccctg
     1081 gccgcgggca acacgatcgt ggccaagccc tccgagctga ctcccctctc gaccctcgcg
     1141 atcgccgagg tcttttcgga gctgccggac ggcacggtga acctgctggc gggagctggc
     1201 gatgtcggcg ccgggatctc cggccatccc gggatcgact gcgtcgcatt taccggttcg
     1261 gttgccaccg gccggaaggt cggcgtcgcg gctgcttccc gcaatgcccg ggtcaacctc
     1321 gagatgggcg gcaaggaccc gttcatcgtc tgcgatgaca tcggcaccga cgggctcgag
     1381 gttgccgccc gtggcggtgc ctgggcggcc tttctgaacg ccggccaggt ctgcacctca
     1441 tccgagcgct tctacgtgat ggagtcggtc ttcgacgagt acgtgagcgc cttcgtcgag
     1501 cacaccaggt cgctagtgat cggcgatccg ctggagtcgg cgaccgacct cgggccgatg
     1561 gtctccgcca cccagcgcgg caaggcgctg ggccaggtcg aggacgctgt cgccggggga
     1621 gccgaactgc tcaccggtgg aggaaatgcc ggattcggga agggccatta cctcgagccc
     1681 tgtgtgctga ccggggttga cgccgcggcc gatctgctcc gcgaagagac tttcgggccg
     1741 gtcgcgccga tcgtgccggt caaggacctc gacgaggcga tcgcgctcgc caacggcctc
     1801 gacttcggcc tcggcgcgaa catctacacc cgcgacctta agcgggcgat ccgctgcgtg
     1861 aaggaggtcc gggcaggttc ggtctggatc aacgaccccc tgaccgacaa tgacgccgga
     1921 cccttcggcg gcttcaagaa ctcaggtatc ggccgcgagc tcggcaccga gggtctagac
     1981 gccttccgcg aagccaagca tgcccacatc gacaccgaga tcgaagtcaa ggactggtgg
     2041 tacccctacg ggtgatcccg ggcggggccg cttccctgcc ggttcaggaa gtggcgccgg
     2101 tggtgccggt gctgcccgcc ggtccggtgg tgccggtcgt gcccgaggcc ccggagctgc
     2161 cacaggtttc gaagccgaag ccgtcggtca gctggtagta ctggccggcc agaatcgaag
     2221 ccttcttgcc cgcggagatc atcgcgtcct tgtcacccga ggcaatcgca tcggctgcct
     2281 ccttcgcgat gtcgacgcgc tgccggccga ggtcgatgat ctgggtcacc gtggtctgat
     2341 cggcagtcgg ggcggtcagg ccctcgagtt catcgagcat ggcggagaaa tccgcggcct
     2401 ggtcctcaaa gtcctgggcg gcatcctcga gctcgccgtt cccgatcgcc tggttgaact
     2461 gttgctcgag tgtggccgaa gttggctggt actgggcgca gatcgcgtca gcgtcagcga
     2521 tgaagccctc ccgcgagagg tcttcttcac tggagccccc gccacagccg gccagcaagg
     2581 cgatcgaaag agcgcagaga aacgcggccg ggatgaaccg cgaagcgcgg gttgtgtcag
     2641 aggggagatt caccatcggc agcctatctc cggacccggc gcggccgtca atagactcgt
     2701 tgccatgtct aacggtcagg acagaactcc ttatgtcgac gcgctgctcg cctttgccca
     2761 gagtgatccg ggcaggttca acgtgccagg tcatcagggc gggatcggtg cggatcccct
     2821 gctgcaccag ctggtcgggc aaaccggcct ttccgatgac atccccggac tgatcgaggg
     2881 catcgacgtt ggtgatccga atccgttcca ggaggcccag gcgctggccg ccggagcatg
     2941 gggcgcgaag cggacctggt ttctgatcaa cggggcctcg cagggcaacc aggcgatgtg
     3001 cctggcggtc gcccacatgg gcggggagct ggtggtccag cggaacgtcc actcctcggt
     3061 catcgacggc atgatcatgg ccgggttgaa gccaaccttc gccgccccgg agcttgaccc
     3121 ggacctcggc gtcgcgcatt gcctgactcc cgacgacctg gccgaggcgc tggaccgctg
     3181 cccggatgcg agtgcggcgg tggtcgtctc acccacctac ttcggggcct gcgccgacat
     3241 ctccggactg gcggaggtcg cccacagccg cggcgttccc ctggttgccg acgaagcctg
     3301 gggggcccac ctccactttc accctgacct gccgcccagc gcgatcgaga gcggcgccga
     3361 cctggtcatt tcctccaccc acaagatcgt tggcagcctg acccagtcgg cgatgatcca
     3421 cctcgccacc gaccgtttcg accaggcgat catcgaccgc tgcatttcga tgaccgagtc
     3481 aaccagcccg aactcgctgc tttgcggttc gctcgatgcc gcccggcggc aggccgccat
     3541 ccatggcgag cggttgctcg gggagacgat cgaagtgctg gtcgaggcgc gggaagagct
     3601 ccgctcgatc cccggcctcg acgtgctcga cgaaaggctg cttgagcgca agagcgtggt
     3661 cggctgggat ccgctccggc tgaccatcga cgtccgggga accggaagca ccgggtacca
     3721 catcgccgag ctggcccggg acctggccgg agtcaatctc gaactggcct cggacacggt
     3781 cgccgtcgcc gtattcggca tgggaacggg caccgcccag cgggcccgcc gcctgatc
//
LOCUS       DAOQKN010000204         3802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_14522_length_3802_cov_5.091006, whole genome shotgun sequence.
ACCESSION   DAOQKN010000204 DAOQKN010000000
VERSION     DAOQKN010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14522_length_3802_cov_5.091006"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="PLJ64_11080"
     CDS             <1..612
                     /locus_tag="PLJ64_11080"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNC94069.1"
                     /translation="AAQEEERARVARDLHDQVNQSLTGVLLRLEATRSEAPEELAPEI
                     EETSRLAHQAMDELLTVARQLRPTALDDLGLRAAIAGLVEQLDRPGLTATFTCDDDPG
                     GLDPDIQLVIYRVSQEAIGNAVRHSGASRVDAVLARNGDRFELKVTDDGSGFSFDQAT
                     SGLGLGGMRERALLVGGELDVESRTGHGTTIRLSVPVREGNPG"
     gene            609..1238
                     /locus_tag="PLJ64_11085"
     CDS             609..1238
                     /locus_tag="PLJ64_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC94070.1"
                     /translation="MRVVIADDHGIVRSGIRLLLERQGDLEVIAEAADGIEARDTVVR
                     ERPDLAILDVKMPGLTGLQATREIKAQTPETNVLILSMHDDERYLFEALKAGASGYVL
                     KSQADTDLLDAIRSVERGEPFLSPGAQQTLIRDLLERGEPAGSDLTPREEEIVKLVAE
                     ANTTREIAGMLHLSEKTVENHRANAMKKLGMRDRVELVRYAIRQGLIEP"
     gene            1235..1924
                     /locus_tag="PLJ64_11090"
     CDS             1235..1924
                     /locus_tag="PLJ64_11090"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC94071.1"
                     /translation="MSPDQVAERVALGPGVEVLIRPIRPTDKHLLADGFAALSPESKF
                     QRFFAPVVRLSESDLVYLTEIDHHHHEALVAIDPDEGDLIGVTRYIRTSPYRDREAEV
                     AIVISDQWRGKGLGKALLSRLATRAREEGVTHFLAVILAENEGSIDLFENFAPKKTHV
                     AAGDPGQVEVRIDLPWSGEFSDSVLGYALRSAARGSHRFRTWRRMRRRMRRRPGREKP
                     AGKVREFGKTD"
     gene            1944..2423
                     /locus_tag="PLJ64_11095"
     CDS             1944..2423
                     /locus_tag="PLJ64_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNC94072.1"
                     /translation="MLFSTKAEYGVRLMVELGRQPNTGPVSLNAIAEAERLPLSYLEH
                     LVAKLRQAGLVTSTRGAHGGYSLAYPAEEITMVEVVEALEGQIAPMECFHETPEGKVL
                     CSHEADSDRACATKLLWTRVQGGVTKALAGTTLAELVEFSRPAEERSKSKTAVGSAA"
     gene            2457..3221
                     /gene="sufC"
                     /locus_tag="PLJ64_11100"
     CDS             2457..3221
                     /gene="sufC"
                     /locus_tag="PLJ64_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HNC94073.1"
                     /translation="MAELEIKNLHVSVEEKEILNGLDLTVEKGRVHALMGPNGSGKST
                     LANVIMGHPALEVTEGSIVFDGEDITDADPDERAQLGLFMAFQYPVSIPGVAVSKYLR
                     MILNAQREARGEQPLKIKEFAAQAREAMDLANIPQDFSSRYLNDGFSGGEKKRMELLQ
                     LALLKPQIAILDETDSGLDIDALRTVAEGINKFAGPDMGALVITHYQRLLHMVEPDVV
                     HVMYEGRIVKEGGKELVDQLEAEGYGWIKEEVEAAA"
     gene            3221..>3802
                     /locus_tag="PLJ64_11105"
     CDS             3221..>3802
                     /locus_tag="PLJ64_11105"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNC94074.1"
                     /translation="MAADSATPAATPTPVRSVDEIRAQFPILAREVKGKPLAYLDNGA
                     TAQKPLAVIEAMDAYYRGHNANVHRGVHTLSEEATDLYEGARAKVAGLLGSTSPREVV
                     FTRNVTSALNLVAQSWGTANLQAGDRILLTEMEHHANIVPWHMIAQRTGAELQFAPID
                     EEGRLDRAALSELLAGGPRVFAFTHVSNVLGTKN"
BASE COUNT          688 a         1325 c         1221 g          568 t
ORIGIN      
        1 gccgcccagg aggaagaacg ggcccgggtc gcccgcgacc tccacgacca ggtcaaccag
       61 tcgctgaccg gcgtgctgct gaggctcgag gcgacccgct ccgaggcccc cgaggagctg
      121 gcgccggaaa tcgaggagac cagccggctc gcgcatcagg cgatggatga gcttctgacc
      181 gtggcccgcc agctgcggcc gaccgcgctg gatgacctcg gtcttcgcgc cgcgatcgcc
      241 ggcctggtcg aacagctcga ccgccccggc cttaccgcca ccttcacctg cgatgacgac
      301 cccggcgggc tcgacccgga catccagctg gtcatctacc gggtctccca ggaagcgatc
      361 ggcaacgcgg tccgccacag cggggccagc cgggtcgatg ccgtccttgc ccgcaacggg
      421 gaccgcttcg agctgaaggt caccgatgac ggcagcggtt tcagcttcga ccaggccacc
      481 tcggggctcg gcctcggcgg catgcgtgag cgggcgcttc tggtcggcgg ggaactcgat
      541 gtcgaatcgc gcaccggcca cgggaccacg atccggttgt cggtcccggt ccgggaagga
      601 aaccccggat gagggtcgtg atcgccgacg accacgggat cgtccgttcc ggaatccggc
      661 tgctgctcga gcgccagggc gatctcgagg tgatcgccga agccgccgac gggatcgagg
      721 cccgcgacac ggtcgtccgc gaaaggcccg acctggccat cctcgacgtg aagatgcccg
      781 gtctgaccgg actgcaggcg acccgcgaga tcaaggcgca gaccccggag accaatgtcc
      841 tgatcctctc gatgcacgat gacgagcgct acctgttcga ggcgctgaag gccggcgcct
      901 ccggttacgt gctcaagtcc caggccgaca ccgacctgct tgacgcgatt cggtcggtcg
      961 agcgcggcga gcccttcctc tcccccggcg cccagcagac tctgattcgc gatctgctcg
     1021 aacgcggcga accggccggg tccgatctca caccgcggga ggaagagatc gtgaagcttg
     1081 tggccgaggc caacacgacg agggaaatcg ccgggatgct ccacctttcc gagaagaccg
     1141 ttgaaaacca ccgggccaac gcgatgaaga agctcggcat gcgggaccgg gtcgaactgg
     1201 tccggtacgc gatccggcag gggttgatcg aaccatgagc cccgatcagg ttgccgagcg
     1261 ggtggcgctc ggccccgggg tcgaggtgct gatccggccg atccgcccga ccgacaagca
     1321 cctgctggcc gacggctttg ccgcactcag cccggaatcg aagttccagc ggttcttcgc
     1381 gccggtcgtt cgcctgagtg aatcggacct cgtgtacctg accgagatcg accatcacca
     1441 ccacgaggcg ctggtggcga tcgaccccga cgaaggagac ctgatcggcg tgacccgcta
     1501 catccgcacc tccccctacc gggaccgcga ggccgaggtg gcgatcgtga tttcggacca
     1561 gtggcgcggc aaaggtctcg gcaaggcgtt gctttcccgg ctcgccacca gggcccggga
     1621 ggagggcgtg acccacttcc tggctgtgat cctcgccgaa aacgagggct cgatcgacct
     1681 cttcgagaac ttcgccccga agaagaccca tgttgcggct ggtgatcccg gccaggtcga
     1741 ggtccgcatc gacctcccct ggagtggcga attctcggat tcggtgctcg gctatgcctt
     1801 gcgctcggcc gcccgcggca gccaccgctt ccgaacctgg cggcgcatga gacgccggat
     1861 gaggcgtcgg ccgggccggg aaaagcccgc tggcaaggtg cgagaattcg gcaaaaccga
     1921 ctagtgctct aggatttata tcgatgctct tctcgaccaa agccgagtac ggagttcgac
     1981 tgatggtcga gctcggtcgc cagcccaaca ccggccccgt ttcgcttaac gcgatcgccg
     2041 aagccgagcg cctgcccctt tcctatctgg agcacctggt ggcgaagctt cgccaggccg
     2101 gtctggtcac ttcgacccgc ggcgcccacg gcggctacag cctcgcctac ccggctgagg
     2161 aaatcaccat ggtcgaggtg gtcgaggccc tcgagggcca gatcgccccg atggagtgct
     2221 tccacgagac cccggaaggc aaggtcctct gctcgcatga agcagacagc gaccgggcct
     2281 gcgcgacgaa gctgctctgg acccgggtcc agggtggcgt gaccaaggcc ctggccggca
     2341 ccacgctggc cgagctggtc gagttctcgc gcccggccga agagcgcagc aagagtaaga
     2401 ccgcggtcgg cagcgcagcc tagccgccgc cccagacttt tcgaacggag caaccagtgg
     2461 cagaacttga aatcaagaac cttcacgtca gcgtcgagga aaaggaaatc ctcaatggcc
     2521 tcgacctgac cgtcgaaaag ggccgggtcc acgcgttgat gggcccgaac ggttccggca
     2581 agtcgaccct cgccaacgtg atcatgggcc accccgccct cgaggtcacc gagggttcga
     2641 tcgtcttcga cggcgaggac atcaccgacg ccgacccgga cgagcgtgcc cagctcggcc
     2701 tcttcatggc cttccagtac ccggtttcga ttcccggcgt ggcggtcagc aagtacctgc
     2761 ggatgatcct caacgcccag cgtgaggccc gcggcgagca gccgctcaag atcaaggagt
     2821 ttgccgccca ggcccgggaa gcgatggatc tcgccaacat cccccaggat ttctccagcc
     2881 gttacctcaa tgacggcttc tccggcggcg agaagaagcg catggagctg ctccagctgg
     2941 cgctgctgaa gccccagatc gcgatcctcg acgagaccga ctccgggctc gacattgacg
     3001 cgctccgtac cgtcgccgag ggcatcaaca agttcgccgg ccccgacatg ggcgccctgg
     3061 tcatcaccca ctaccagcgc ctgctccaca tggtcgagcc ggatgtggtt cacgtgatgt
     3121 acgaaggccg catcgtcaag gaaggcggca aggaactcgt tgaccagctc gaggccgagg
     3181 gctacggctg gatcaaggag gaggtcgagg cggccgcctg atggcagccg actccgccac
     3241 ccccgctgcc acaccgaccc cggtccggtc ggtcgacgaa atccgcgcgc agttcccgat
     3301 cctcgcccgc gaggtaaaag gaaagccgct cgcgtatctg gacaacggcg ccaccgccca
     3361 gaagccgctg gcggtgatcg aggcgatgga cgcctactac cgcgggcaca acgccaacgt
     3421 ccaccgcggc gtccacaccc tctccgagga agccaccgac ctctacgagg gcgcgcgcgc
     3481 aaaggtcgcc gggctgctcg gatcgacctc cccgcgcgag gtggtcttca cccgcaatgt
     3541 gacctcggcc ctcaacctcg tcgcccagtc ctggggcacg gccaacctcc aggcgggcga
     3601 ccgcatcctg ctgaccgaga tggagcacca cgcaaacatc gtgccctggc acatgatcgc
     3661 ccagcggacc ggtgccgagc ttcagttcgc gccgatcgac gaggagggcc ggcttgaccg
     3721 ggccgcgcta tcggaactgc tggccggtgg ccccagggtc ttcgccttca cccatgtctc
     3781 gaacgtgctc ggcaccaaga ac
//
LOCUS       DAOQKN010000205         3799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_14546_length_3799_cov_3.993857, whole genome shotgun sequence.
ACCESSION   DAOQKN010000205 DAOQKN010000000
VERSION     DAOQKN010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14546_length_3799_cov_3.993857"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="PLJ64_11110"
     CDS             complement(<1..212)
                     /locus_tag="PLJ64_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94075.1"
                     /translation="MSEGTTIVRGAWALTMGPAGAIREGAVAFDGDGAISAVGPADQL
                     IAADPGAELVGDGNGIVLPGFVNAHT"
     gene            complement(209..1033)
                     /locus_tag="PLJ64_11115"
     CDS             complement(209..1033)
                     /locus_tag="PLJ64_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC94076.1"
                     /translation="MFARGTRARRIVAAVFPPLLFAIFVIGAWQLYTVIGDVKESTLP
                     PPTAIAEALWNDRSMLIENAWVTIKEILLGFAAAIVLGVGLAVLIRSSRTIERAVYPW
                     LVVSQMVPIPAIAPLLIIWFGFEIEPKVIVIMLVSFFPIAVNTIDGLRAAEPELLNLL
                     KTLKANSWQRFRKAQLPAALPFLFSGLKVAAALSVIGAVFAEWVGASAGLGYLILVLN
                     NQTATTEMFAVIVTLASIGILLFLIVTLLERLLLPWYYESRETEGEGLHLEVEEKA"
     gene            complement(1033..1857)
                     /locus_tag="PLJ64_11120"
     CDS             complement(1033..1857)
                     /locus_tag="PLJ64_11120"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC94077.1"
                     /translation="MQAGSKLKIEGLSKTYRGAGGGENVTALKDVGIEIGAGEFVSIV
                     GPSGCGKSTLFNIIAGLIRPSSGKVLLDGRQPDELLGSVGYMPQRDCLMPWRTVLDNT
                     TLGLELNGVRKKEAHKKALAEFERFGLAGFEKHWPAKLSGGMKQRAALLRTFLAGRDV
                     MLLDEPFGALDALTRANMQQWLLDVWQEDRKTILFVTHDVEEAIYLSDRVYVMSGRPG
                     RVSLDVNVNLERPRKPEITMSPEFLVLKQKLLRPLEEAAAGGGLFVDDRPTLEEQI"
     gene            complement(1890..2921)
                     /locus_tag="PLJ64_11125"
     CDS             complement(1890..2921)
                     /locus_tag="PLJ64_11125"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNC94078.1"
                     /translation="MSFTTSWKKIMLLLAATMALAVGVAACGSSDDDSGDSGSSTAGG
                     TQDATLVLDFVPGAVHSGIYEAVQKGYYEDNGINLKIIEPTSTADTLKLIDAGKAEFG
                     IADGIDLATQISDGREAQGIMAIAQRPPGGLITLEESGITDPKQLEGQTVGITGVPSD
                     TAILNTVMKDAGGNPDDADVVTIGFNGVQALESGKVAAFTGFIPADGVQVEADGYPTK
                     SFPLDEYGGPSYPGLVVFSTESKIGDDPDLMQGFVDATIKGYQDALADPAQAIDALIS
                     QTQGIDKQLATKSFEAWTPLLGPADTFGQFNKKNLESLSTFMVDNDLASEPISPDRYA
                     TTDFTQPKE"
     gene            complement(2992..3624)
                     /locus_tag="PLJ64_11130"
     CDS             complement(2992..3624)
                     /locus_tag="PLJ64_11130"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNC94079.1"
                     /translation="MAEAARIITGAGLVEAFGHVSARAGDGFLITATRPLGGAGERDV
                     YRVDADGEAPEGATDLPLETPMHAAVYAARPEVQAICRTHSPAAVRAGARQVVPPLVH
                     GLGGLAGEVALCGRTDLVTDREAGGEISRDLGSADCLLIRGNGSIATGRSLAEAVVRA
                     RYLEERCLAGAGMGQEEGWRADLVDPRARWFEKETTRAWAWMRWRYGGEK"
BASE COUNT          620 a         1274 c         1246 g          659 t
ORIGIN      
        1 tgggtgtggg cgttgacgaa gccgggcagg acgattccgt tgccgtcacc gaccagctcg
       61 gcgccgggat cggcggcgat cagctgatcg gccgggccga ccgcggagat cgcgccgtcc
      121 ccgtcgaagg cgaccgcacc ctcgcggatc gccccggccg ggcccatggt cagggcccag
      181 gcgccacgga cgatggtcgt gccctcgctc acgccttctc ctcgacttcg aggtggaggc
      241 cctcgccctc ggtctcacgg gactcgtagt accacggcag cagcagccgc tcgagcaggg
      301 tcacgatcag gaagagcaag atgccgatcg aggccagggt cacgatcacg gcgaacatct
      361 ccgtggtcgc ggtctggttg ttcaacacga ggatcaggta gccgaggccg gccgaggcgc
      421 caacccattc ggcgaagacg gcgccgatga ccgaaagcgc ggcggcaacc ttcaggccgg
      481 agaaaaggaa aggcagcgcg gccggcagct gcgccttgcg gaaacgctgc caggagttcg
      541 ccttcagggt cttcaggaga ttgagcagtt cgggctcggc cgcccgcagg ccgtcgatcg
      601 tgttgacggc gatcgggaag aagctgacca gcatgatcac gatcaccttc ggctcgatct
      661 cgaagccgaa ccagatgatc agcagcgggg cgatagccgg gatcggcacc atctgagaga
      721 cgaccagcca aggatagacc gcccgctcga tcgtccgtga gctccggatc agaacggcga
      781 ggccgacccc gagcacgatc gccgcggcaa agccgagcag gatctccttg atcgtcaccc
      841 aggcgttctc gatcagcatc gagcggtcgt tccagagcgc ctcggcgatc gcggtcggcg
      901 gcggcagggt cgattccttg acgtcgccga tcaccgtgta gagctgccag gccccgatca
      961 cgaagatcgc gaacagcagc gggggaaaca ccgccgcgac gatgcggcgg gcgcgagttc
     1021 ctcgggcaaa catcagatct gctcctccag ggtcggtcgg tcatcgacga agagacctcc
     1081 gccggccgcg gcctcctcga gtggcctgag cagtttctgc ttcaagacca ggaactcggg
     1141 tgacatcgtg atctcaggct tgcgtggccg ttccaggttc acgttcacgt cgagggagac
     1201 gcgacccggc cggccggaca tcacgtagac ccggtcggag aggtagatcg cctcctcgac
     1261 gtcatgggtc acgaaaagaa tggtcttgcg gtcctcctgc cagacgtcca gcagccactg
     1321 ctgcatgttg gcccgggtca acgcgtccag ggcgccgaag ggctcgtcga gcagcatcac
     1381 gtcacggccg gcgaggaagg tgcgcagcag ggcggcacgc tgtttcatgc cgccggagag
     1441 cttggccggc cagtgcttct cgaagccggc caggccgaag cgctcgaact cggccagcgc
     1501 tttcttgtgc gcttccttct tgcgcacgcc gttcagctcc agcccgagcg tcgtgttgtc
     1561 gagcaccgtg cgccagggca tgaggcagtc gcgctggggc atgtacccga ccgagccgag
     1621 cagttcgtcg ggttgccggc cgtcgagcag caccttgccg ctcgacggcc ggatcagccc
     1681 ggcgatgatg ttgaagaggg tcgacttgcc acatccagag ggacccacga tcgagacgaa
     1741 ttcgccggct cctatttcga tgccgacatc cttcagcgcg gtgacgttct cgccgccacc
     1801 agcgccccga taggtcttcg atagaccttc gatcttgagt ttcgaacctg cctgcaagtc
     1861 aatcctcctt tgcttctctt acgccctgat cactctttgg gctgggtgaa atcggtggtc
     1921 gcgtaacggt ccggcgagat cggttcgctg gccaggtcgt tgtccaccat gaacgtggag
     1981 agtgactcca ggttcttctt gttgaactgg ccgaaggtgt cggccggtcc gagcagcggg
     2041 gtccaggcct cgaacgactt cgtggccagc tgcttgtcga tgccctgggt ctgcgagatc
     2101 agcgcgtcga tcgcctgggc cggatccgcg agcgcgtcct ggtagccctt gatcgtcgcg
     2161 tcgacgaagc cctgcatcag gtccgggtca tcgccgatct tcgactcggt cgagaagacg
     2221 accagacccg ggtaggacgg tccgccgtac tcgtcgagcg ggaacgactt ggtcggatag
     2281 ccatcggctt cgacctggac gccatcggcc gggataaagc cggtgaaggc cgccaccttg
     2341 ccggactcca gcgcctggac gccgttgaag ccgatcgtga ccacatcggc gtcatccggg
     2401 ttgccgcctg cgtccttcat cacggtgttg aggatcgccg tgtccgacgg aacgccggtg
     2461 atgcccacgg tctggccttc gagctgcttc ggatcggtga tgccgctttc ctcgagggtg
     2521 atcaggccac ccgggggccg ctgggcgatc gccatgatgc cctgggcttc ccggccgtcg
     2581 ctgatctggg tcgccaggtc gatgccgtcg gcgatgccga actcggcctt gccggcgtcg
     2641 atcagcttca gcgtgtcggc ggtcgaggtc ggctcgatga tcttgaggtt gatgccgttg
     2701 tcctcgtagt agcccttctg gacggcctcg tagatgccgg agtgaacggc gccgggcacg
     2761 aagtccagca ccagggttgc gtcctgggtg ccaccggccg tgctggagcc ggagtcaccc
     2821 gagtcgtcgt cgctcgagcc acaggcggcg accccgacgg cgagcgccat cgttgcggcc
     2881 agcagcaaca tgatcttctt ccaactggtt gtgaaactca tgagtcctct ctctggttgt
     2941 agttcgaaac acccgccccg gttctcaccc gggaccggtt caacgcgatc cctatttttc
     3001 tccgccgtac ctccatcgca tccaggccca ggcccgggtc gtttcctttt cgaaccatct
     3061 ggcacgggga tccacgaggt cggccctcca accttcctcc tggcccatac ccgctccggc
     3121 cagacacctt tcctccagat agcgggcccg gaccaccgct tcggccaggc tgcgcccggt
     3181 cgcgatcgaa ccgttcccgc ggatcagcag gcaatccgcg ctgccaagat ctcttgagat
     3241 ctcgcccccg gcctcccgat cggtgaccag gtcggtgcgg ccgcagagcg cgacttcacc
     3301 ggccagcccg ccgagaccat gcaccagggg cggcacgacc tgccgggcgc cggcccgcac
     3361 cgcggccggc gaatgggtcc ggcagatcgc ctggacctcg ggccgggccg catagaccgc
     3421 cgcgtgcatc ggcgtctcga gcggcaagtc ggtcgccccc tcgggcgctt cgccgtcggc
     3481 gtcgacccgg tacacgtccc gctcgccggc tccgccgagc gggcgggtag cggtgatcag
     3541 gaagccgtca cccgcgcgcg ccgaaacgtg gccgaaggct tcgaccaggc ctgccccggt
     3601 gatgatccgg gccgcttcgg ccacctcggc cgcgctcgta tgagtcgatc cagtcagtcg
     3661 ctcaccctca tcgccatcgg ggtcgggttg aaggcattta ccgcggagat gtcctggggg
     3721 caggccgaaa gggcgacggt catgtcttcc agcacctcca tcgcgatcgt cgatccggcc
     3781 ggatggggcg gctcgtaag
//
LOCUS       DAOQKN010000206         3762 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_14798_length_3762_cov_6.551929, whole genome shotgun sequence.
ACCESSION   DAOQKN010000206 DAOQKN010000000
VERSION     DAOQKN010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14798_length_3762_cov_6.551929"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="PLJ64_11135"
     CDS             complement(<1..927)
                     /locus_tag="PLJ64_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94080.1"
                     /translation="MPKKEDRARPAGDGEAGKPARPALTEKQRIEARRARQSRSHRKS
                     PEGNALSRGLRATGFEVRRTASFIGGGVIAALAALGPLFSSAGMGLVWLIERIGEGLK
                     VLGRVTGRAVAALGRAVVAADRVITPHRALLLVAAIAAVLLAVSQFKGLGQIEIGQSG
                     YSGFQDLARAPAIDHTTPAGVHTKILVPIAGLALAAVAVILLGGLASTARRFSRFRRL
                     ASMVLVTIGLLTLAVALLVDLPDARDTTEAALAYSGVKAVLLSGFWLELAAGAALTVT
                     GFALLFEPSPGRAREKRPQEEPGSRPGPGNPDE"
     gene            1220..3331
                     /gene="fusA"
                     /locus_tag="PLJ64_11140"
     CDS             1220..3331
                     /gene="fusA"
                     /locus_tag="PLJ64_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNC94081.1"
                     /translation="MARSFPLEKTRNIGIMAHIDAGKTTTTERILYYTGRTHKIGEVH
                     EGAATMDWMEQEQERGITITSAATACEWLGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VAVFDSVAGVEPQSETVWRQADKYSVPRIAYINKMDRTGADFYRSVETIKDRLGANAV
                     PIQLPIGAEGEFAGIIDLVTMKATIYKDDMGQELEVVEIPADMADQADEYRTALLEAV
                     ADQDDELMELYLEGEEIAEDRIIAALKAATLAIDVTPVLCGSSFKNKGVQPLLDNIVA
                     LLPSPLEVPPVEGMLPLKKGEEERQEGNRPADDSGPFAALAFKVMADPYVGKLTYFRV
                     YSGSLEAGGRVLNVTTGKTERIGRLLMMHANSREEIERVYSGDICAGVGLKQTGTGDT
                     LSAPDAPIELESMDFPDTVIAVAIEPKTKSDQEKLGMALQRLAEEDPTFKVESDEETG
                     QTLIHGMGELHLEVLVDRMIREFNVEANVGKPQVAYRETIRKDVPKVSGKFARQTGGR
                     GQYGHAVINIEPAPGEGFIFDNKIKGGVIPTEYIPAVQQGIKEALENGVKAGYPMVDV
                     KVELIDGSYHDVDSSEMAFKIAGSMALQEAARKASPTLLEPVMAVEVTTPEDFLGDVI
                     GDLSRRRGKVQGQEQRGNALAVQAYVPLSEMFGYATDLRSSTQGRAQYTMQFERYEEV
                     PSNIAEEIAESRSGAKAGAEA"
     gene            3480..>3762
                     /locus_tag="PLJ64_11145"
     CDS             3480..>3762
                     /locus_tag="PLJ64_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNC94082.1"
                     /translation="MSKEKFERDKPHVNVGTIGHVDHGKTTLTAAITNTLSEDKKSFE
                     EIDNAPEEKERGITIATSHVEYETPNRHYAHVDCPGHADYVKNMITGAAQ"
BASE COUNT          753 a         1251 c         1219 g          539 t
ORIGIN      
        1 ctcgtcgggg ttaccggggc cgggccggct gccgggctcc tcctgcggcc gcttctccct
       61 ggcccggccg ggcgagggct cgaagagcag ggcgaagccg gtcacggtca gggcggcccc
      121 ggccgcaagt tcaagccaga agccggaaag caggaccgcc tttacccccg agtaggcaag
      181 cgcggcttcg gtcgtgtcgc gggcgtcggg aaggtcgacc aggagggcca cagccagggt
      241 caacaggccg atcgtgacca ggaccatcga ggcgaggcga cggaagcggc tgaaccgcct
      301 ggccgtggag gcgaggccgc cgagcaggat cacggcgacc gcagccaggg cgagcccggc
      361 gatcggaacc aggatcttcg tgtggacgcc ggccggggtg gtgtggtcga tcgccggggc
      421 gcgggcgagg tcctggaaac cggagtagcc ggactggccg atctcgatct ggcccaggcc
      481 cttgaattgc gagacggcga gcagcacggc cgcgatcgcc gcgaccagga gcagagcccg
      541 gtggggggtg ataacccggt cggccgcaac gacggcacgg cccagcgccg cgacggcccg
      601 cccggtcacg cgccccagaa ccttgaggcc ctcgccgatc ctttcgatca gccagaccag
      661 gcccatgccg gccgaagaga agaggggccc gagtgccgcc agggcggcga taacgccgcc
      721 gccgatgaaa gaagccgtgc ggcggacctc gaaaccggtc gcccttagac cccgggaaag
      781 ggcatttcct tcgggggatt tgcggtggct tctggactgg cgggcccggc gcgcctcgat
      841 tcgctgcttc tcggtcaggg ccggtcgcgc gggcttgccc gcctcgccgt caccagccgg
      901 cctggcgcga tcttcttttt tcggcatgtc cgggtccaca gcggtctctc ctccggggat
      961 gaatcaggct ccagagtatt ggagtcggag gtcggcagta ccttgattgg accggccttc
     1021 ctctgctata gtcgcccgtc gctcgggtca gccctgcatc ctgcggggct ttttgttggc
     1081 ccgctgcccg ttggccggac tccagctggg tctgaccgac agaagaccga cagaaaatca
     1141 agaggttggc ctcgccgcga agcggtccgg aaggtcagcc ggtaacgcaa cggaaacttc
     1201 caggaaccag agagaaaaag tggcgcgctc cttccccctt gaaaaaaccc gaaacatcgg
     1261 gatcatggct cacatcgacg ccggtaagac gacgacgacc gagcgcatcc tctactacac
     1321 cggccgaacc cacaagatcg gcgaggttca cgagggcgcc gcgaccatgg actggatgga
     1381 gcaggagcag gagcggggga tcaccatcac ctcggctgct accgcctgcg agtggctcgg
     1441 ccaccgcatc aacatcatcg acacccccgg ccacgtcgac ttcacggtgg aagtcgagcg
     1501 ttcgctgcgc gtgctcgacg gcgccgtcgc cgtcttcgac tcggttgccg gcgtcgagcc
     1561 gcagtccgag accgtctggc gccaggctga caagtactcg gttccgcgaa tcgcctacat
     1621 caacaagatg gaccgcaccg gcgcggactt ctaccgctcg gtcgagacca taaaggaccg
     1681 cctcggtgcc aacgccgtgc cgatccagct gccgatcggt gccgaaggcg agttcgccgg
     1741 aatcatcgac ctggtcacca tgaaggccac catctacaag gacgacatgg gccaggagct
     1801 cgaggtcgtc gagattccgg ccgacatggc cgatcaggcc gacgagtacc gcacggcgct
     1861 gctcgaagcc gtcgccgacc aggacgacga gctgatggag ctttacctcg aaggcgagga
     1921 aatcgccgag gaccgcatca tcgcggcact caaggcggcc accctggcga tcgacgtaac
     1981 cccggtcctt tgcggctctt cattcaagaa caagggcgtg cagccgctgc tcgacaacat
     2041 cgtcgcgctg ctgccttccc cgctcgaggt tccccccgtg gaaggcatgc tgccgctgaa
     2101 gaagggcgag gaagagcgtc aggagggcaa ccgcccggcc gacgactccg gccccttcgc
     2161 cgcgctcgcc ttcaaggtga tggccgaccc ctacgtcggc aagctgacct acttccgcgt
     2221 ctactccggc agcctcgaag ccggcggccg cgtgctgaac gtgaccaccg gcaagaccga
     2281 gcggatcggt cgcctgctga tgatgcacgc caactcgcgc gaggaaatcg agcgggtcta
     2341 ctccggtgac atctgcgccg gcgtcggcct caagcagacc ggtaccggcg acacgctgtc
     2401 ggcccccgat gccccgatcg agctcgagtc gatggacttc ccggacaccg tgatcgcggt
     2461 cgcgatcgag ccgaagacca agtccgacca ggagaagctg ggcatggccc tgcagcgcct
     2521 ggccgaagag gacccgacct tcaaggtcga atccgacgag gaaaccggtc agaccctgat
     2581 ccacggcatg ggcgagctgc acctcgaggt gctggtcgac cggatgatcc gtgagttcaa
     2641 cgtcgaagcc aacgtcggca agccgcaggt cgcctaccgc gagacgatcc gcaaggacgt
     2701 gcccaaggtg agcggcaagt tcgcccgcca gaccggcggt cgcggccagt acggccacgc
     2761 ggtcatcaac atcgagcccg cccccggcga aggtttcatc ttcgacaaca agatcaaggg
     2821 cggcgtgatt ccgaccgagt acatcccggc tgtccagcag ggcatcaagg aagcgctcga
     2881 gaacggcgtc aaggccggct acccgatggt tgacgtgaag gtcgagctga tcgacggctc
     2941 ctaccatgac gtcgactcct cggaaatggc cttcaagatc gccggttcga tggcgcttca
     3001 ggaagctgcc cgcaaggcca gcccgaccct gctcgagccg gtcatggcgg tcgaggtcac
     3061 cactcccgag gacttcctcg gcgacgtgat cggcgacctt tcgcgccgtc gcggcaaggt
     3121 ccagggccag gagcagcgcg gcaacgcgct cgccgttcag gcttacgtgc cgctcagcga
     3181 gatgttcggt tacgcgaccg acctgcggtc ttcgacccag ggccgggccc agtacacgat
     3241 gcagttcgag cgctacgaag aggtgccgag caacatcgcc gaagagatcg ccgagagccg
     3301 ttcaggcgca aaggccgggg cagaagccta ggccgtaaat accccgccgg ggctaaatgg
     3361 ctatatttca gaggttcgcc caaaatgggc gaaagtccgg agccaccaag ctttagcagt
     3421 tcaatcagac gtagaaacca agatccaaga gaaacagacg agaaaggtcc caggagagca
     3481 tgtccaagga gaagttcgag cgcgataagc cgcacgtaaa cgtcggcacc atcggtcacg
     3541 tcgaccacgg caagaccacc cttaccgcgg ccatcaccaa cactctctcc gaagacaaga
     3601 agagcttcga ggagatcgac aacgctccgg aagagaagga gcgcgggatc accatcgcga
     3661 cctctcacgt cgagtacgag accccgaacc ggcactacgc ccacgtcgac tgccccggcc
     3721 acgccgacta cgtcaagaac atgatcaccg gtgccgccca ga
//
LOCUS       DAOQKN010000207         3717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_15111_length_3717_cov_7.128891, whole genome shotgun sequence.
ACCESSION   DAOQKN010000207 DAOQKN010000000
VERSION     DAOQKN010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15111_length_3717_cov_7.128891"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1659
                     /gene="glsA"
                     /locus_tag="PLJ64_11150"
     CDS             <1..1659
                     /gene="glsA"
                     /locus_tag="PLJ64_11150"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF016824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HNC94083.1"
                     /translation="TRREFTIQSISKPLTFALALEAIGEEEVRRHVDVEPSGEAFNSI
                     TLQPGTGLPLNPMVNAGAIATASLIEPLGFEKPIDRILHEYSQFAGRHLKVDEQVFTS
                     ENETGNRNRAIAYLLKNAGVIDRDVEEVVETYFRQCSTLVTCRDLATMAATLASTGVN
                     PVTGERVVREDTVRNVLSVMASCGMYDAAGDWLYTVGLPAKSGVAGGIIAVLPGQLGV
                     AVYSPPLDLHGNSVRGVKVCEDLSDELHLHVMSPARRPPPPVRIERTCIDSRSKRLRG
                     PAEQQILAEHGRRVKVLELQGELSFAAGELVTSSALHRAEHHDFTVLDFRAVRAIDLI
                     DVPIFSHLVESCEEAGSEIAFSDVDRHPVFLEELTRHREREGHHPLRTFPDLDLALEW
                     CEQGLIEKYGDLESDPELSLAQHGTTLGMSVEEIEELSSHLEHVSYEAGEMIYGAELV
                     APDQMLLVTAGKISLLAEDDEGGHRRLATLSPGMTLGEISMIAGTPLAGMPRADTEVK
                     AWVLTAGDLEVLRARNPGLVVTLLENLLRQVAQRVGHLRAELLR"
     gene            complement(1663..1917)
                     /locus_tag="PLJ64_11155"
     CDS             complement(1663..1917)
                     /locus_tag="PLJ64_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94084.1"
                     /translation="MPAAYQAGDLQQIFQRINDRIRAIEEHLVVLSEKAGVAYSLPGE
                     GLPKEVIELARAGKTLEAIKLYRELTNADFETAKAAVQAV"
     gene            complement(1991..3067)
                     /gene="ychF"
                     /locus_tag="PLJ64_11160"
     CDS             complement(1991..3067)
                     /gene="ychF"
                     /locus_tag="PLJ64_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003026825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNC94085.1"
                     /translation="MALSIGIVGLPNAGKSTLFNALTKNDVLAANYPFATIEPNVGVV
                     GVPDPRLEKLAGIYGSEKILPATVQFVDIAGIVKGASEGEGMGNKFLSNIRESDAICQ
                     VTRVFRDDDITHVDGEVEPKNDIEVIRTELILADLQTIENALPRLTKEAKRDKDLEPQ
                     VKAAGEAKEILEGGKGIFEAGFDPEQISDLHLMTAKPLLYVFNCSEDELADESLKDEL
                     RQLVTPAEAIFLDAKFEAELVELDDEEEAREMLADIGVEEPGLDVLARVGFETLGLQT
                     YLTAGPKETRAWQIHKGDTAPQAAGVIHTDFQRGFIKAEIISFDDLLEFGSIADARAA
                     GKVRLEGKDYVMQDGDVVDFKFNV"
     gene            3217..>3717
                     /locus_tag="PLJ64_11165"
     CDS             3217..>3717
                     /locus_tag="PLJ64_11165"
                     /inference="COORDINATES: protein motif:HMM:NF040071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6351 family protein"
                     /protein_id="HNC94086.1"
                     /translation="MKQRYLRILTTKTLVFASLIASLTTGVSVAGAEVAPGLLDQLTV
                     RAVSSRADMVSGGDAIVQVRVPASADPGDARIYQDTGNITPAFSPVAGSPNLLQGRVN
                     GIGPGTVIRVGLPGFQTQRLSIVNHPLSGPVLSGRHLNPLVCNTAAAGLGAPQDENCT
                     GNTVVTY"
BASE COUNT          620 a         1213 c         1228 g          656 t
ORIGIN      
        1 acgcggcggg agttcacgat ccagtcgatc tccaagccct tgacctttgc cctggcgctg
       61 gaggcgatcg gggaagagga agttcgccgg catgtcgacg tggagccgag cggggaggct
      121 ttcaactcga tcaccctgca gcccgggacc gggctgccgc tgaacccgat ggtcaacgcc
      181 ggggcaatcg ccacggcctc gctgatcgag ccgctcggat tcgagaagcc gatcgaccgg
      241 atcctccatg agtactcgca gttcgccggc cgccacctga aggtcgacga gcaggtcttc
      301 acctccgaga acgagaccgg aaaccgcaac cgggcgatcg cctatctgct gaagaacgcc
      361 ggggtgatcg accgggacgt ggaagaggtg gtcgaaacct atttccgcca atgctccacc
      421 ctggtcacct gccgggacct ggcgaccatg gcggccacac tcgcttcgac cggggtcaac
      481 ccggtgaccg gcgagcgggt ggtccgggag gacacggtcc gcaacgtgct cagcgtgatg
      541 gcctcctgcg gcatgtacga cgcggccggc gactggctct acacggtcgg cctgccggcg
      601 aagtccggcg tcgccggcgg gatcatcgcg gttctgcccg gccagctcgg ggtggccgtc
      661 tactcgccgc cgctcgacct gcacggcaac agcgttcgcg gggtcaaggt ctgcgaagac
      721 ctctccgacg agcttcacct gcatgtgatg agcccggccc ggcggccacc gcctccggtc
      781 aggatcgagc ggacctgcat cgactcgcgg tcaaagcgtt tgcggggccc ggccgagcag
      841 cagatcctcg ccgagcatgg ccgccgggtg aaagtcctcg aactgcaggg cgagctttcc
      901 ttcgcggccg gcgagctggt caccagttcg gccctccacc gcgccgaaca ccacgacttc
      961 acggtgctcg acttccgggc ggtcagggcg atcgacctaa tcgacgtgcc gatcttcagt
     1021 cacctggtcg agagctgcga ggaggccggc agtgaaatcg ccttctcgga tgtcgaccgc
     1081 catccggtct ttctggaaga gctgacccgg catcgggaac gcgaaggcca ccacccgctc
     1141 aggaccttcc cggacctcga cctcgccctc gaatggtgcg agcaggggct gatcgagaag
     1201 tacggcgacc tcgaatccga cccggaactc agcctcgccc agcacggtac gaccctcggc
     1261 atgagcgtcg aagagatcga ggagctgagt tcacacctcg agcacgtttc ctacgaggcg
     1321 ggggagatga tctacggagc ggagctggtc gcacccgacc agatgctgct ggtcacggca
     1381 gggaagatca gcctgctggc cgaggacgat gaaggcgggc accggcgcct ggcgacgctc
     1441 tcaccgggga tgaccctggg cgagatctcg atgatcgccg ggacgccgct ggccggcatg
     1501 ccccgcgccg acaccgaggt caaggcgtgg gttcttaccg caggcgacct cgaggtgttg
     1561 cgggcgagga acccggggct ggtcgtgacc ctgctggaga acctgctgag gcaggtcgcc
     1621 cagcgggtcg gtcaccttcg cgccgagctc ctgcgctgac cgtcagaccg cctgaacggc
     1681 cgccttcgcg gtctcgaagt cggcgtttgt cagctcgcgg tagagcttga tcgcttccag
     1741 ggtcttgccg gcgcgggcca gttcgatcac ttccttcggc aagccttcgc cgggcagcga
     1801 gtaggcaacc ccggccttct ccgaaagcac gaccaggtgc tcctcgatcg cccggatgcg
     1861 gtcgttgatc cgctggaata tctgctggag atctccggcc tggtaggcag ctggcattga
     1921 cttccccctt cgattgactg gatgtcagcc gagcataacc ggggtgtccg gaagccgggc
     1981 gctttcgcgg tcagacgttg aacttgaagt cgaccacgtc gccgtcctgc atcacgtagt
     2041 ccttgccttc gaggcggacc ttgccggcgg cccgggcgtc ggcgatcgaa ccgaactcga
     2101 gcaggtcgtc gaaggagatg atctcggcct tgatgaagcc gcgctggaaa tcggtgtgga
     2161 tgacgccggc ggcttgcgga gcggtgtcgc ccttgtggat ctgccaggcg cgggtttcct
     2221 tcgggccggc ggtgagatag gtctgcaggc cgagggtctc gaacccgacc cgggccagca
     2281 cgtcgaggcc cggttcctcg accccgatgt cggcgagcat ctcgcgggct tcctcctcgt
     2341 catcgagctc gaccagttcg gcctcgaact tggcgtcgag gaagatcgcc tcggccgggg
     2401 tgaccagctg gcgcagctcg tccttcagcg actcgtcggc gagctcgtcc tcggagcagt
     2461 tgaagacgta aagcagcggc ttggcggtca tcagatgaag gtcgctgatc tgctccgggt
     2521 cgaagccggc ctcgaaaatc cccttgccgc cttcgaggat ctccttcgcc tccccggccg
     2581 ccttgacctg cggttcgagg tccttgtccc gtttggcttc cttggtcagc ctcggcagcg
     2641 cgttctcgat cgtctgaagg tcggcgagga tcagctcggt gcgaatcacc tcgatgtcgt
     2701 tcttcggttc gacttcgccg tcgacatggg tgatgtcgtc atccctgaag acccgggtca
     2761 cctggcagat cgcatccgat tcgcggatgt tcgaaaggaa cttgttgccc atgccctcgc
     2821 cctccgaagc acccttgacg atcccggcga tgtcaacgaa ctggaccgtg gcgggaagga
     2881 tcttctccga accgtagatt cccgcgagtt tctccaggcg tggatccggg acgccgacca
     2941 caccaacgtt gggctcgatc gtcgcgaatg gatagttcgc ggcgagcacg tcgtttttgg
     3001 tcagggcatt gaagagggtc gacttcccgg cgttgggaag gccgacgatt ccgatcgaga
     3061 gggccatcgc cgggaagact acgggcccgg cagcgagtgg ggcggcaagg tcaccgcaag
     3121 gccggttcac gaagatgcgg gcatgaataa accgcaactc gtccggagcg ggcaagtttc
     3181 agaggtcgac atgccaattc gtcccggggg ccggccttga agcagcgtta cctgcgcatc
     3241 ctgaccacaa agacgcttgt cttcgcgtcg ttgatcgcgt cgttgacgac gggggtttcc
     3301 gtggccggcg ccgaagtcgc acccggcctg ctggatcaat tgacggtgcg tgccgtttcc
     3361 tcccgggccg acatggtcag cggcggagac gcgatcgtcc aggtcagggt tcccgcctcg
     3421 gccgatccgg gagacgcgag gatctaccag gacaccggca acatcacgcc tgctttcagt
     3481 ccggtcgccg gcagcccgaa cctgcttcag ggccgggtga acgggatcgg gccgggcacc
     3541 gtcatccggg tcggcctgcc tggtttccag acccagcggc tcagcatcgt gaaccatccc
     3601 ctgagcggcc cggtcctttc gggccgccac ctgaatccgc tggtctgcaa cacggcggcg
     3661 gccggtctcg gagctcccca ggacgagaac tgcaccggga acaccgtcgt cacctat
//
LOCUS       DAOQKN010000208         3681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_15371_length_3681_cov_6.124931, whole genome shotgun sequence.
ACCESSION   DAOQKN010000208 DAOQKN010000000
VERSION     DAOQKN010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15371_length_3681_cov_6.124931"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="PLJ64_11170"
     CDS             <1..456
                     /locus_tag="PLJ64_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC94087.1"
                     /translation="LDEPTTGFDPSARRQAWEMVAGLRGLGKTVLLTTHYMEEAQFLA
                     DRVAIMSEGRIVALDTPENLGDRRRMPTTISFSVPAGTDLAGIAGVGQTQSSSEDDTG
                     LRTVSIGTHEPVADLNRITGWAIESDLEIQHLEVRRPSLEDVYLELTDA"
     gene            453..1223
                     /locus_tag="PLJ64_11175"
     CDS             453..1223
                     /locus_tag="PLJ64_11175"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC94088.1"
                     /translation="MKPISLALHEFRFAQKVFWRNPASVFFTALLPVLFLLIFATIFG
                     GDQIEELGGVKVTTYYIPAIVTLAVISATMVNLSMNVTIAREQGLLKRGRGTPLPPWV
                     FIAGRVGNAIVISAVMLVVVTLIGKLLYDAPIPWERIPAVLVTLVVGATSFCCLGLAM
                     TVIIPSREAASAISNAITLPLYFLSGIFIPENEIPEGVLSFANHFPVRDFFEAFFSAY
                     SPKTVGAGFEWGELAVVAIWGLAGFLIALRYFRWTPRQ"
     gene            1337..1699
                     /locus_tag="PLJ64_11180"
     CDS             1337..1699
                     /locus_tag="PLJ64_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94089.1"
                     /translation="MIHHIVLEVSDLPRASSFYDALLSPLGWRRHVNETNAVGYGISR
                     PILFITERTGVRAEGTMVSLGAPGIAAVKAAWEGGTNSGGSNVAQPGETSAHGSGSYS
                     AFLRDPDGHDIEITVSQD"
     gene            2006..3007
                     /gene="gap"
                     /locus_tag="PLJ64_11185"
     CDS             2006..3007
                     /gene="gap"
                     /locus_tag="PLJ64_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNC94090.1"
                     /translation="MSVKVAINGFGRIGRNVFRAAHDSGADLDIVAINDLVDTETLAH
                     LLKYDSILGRLDAEVSAGEGSITVDGKEIKVLAERDPADLPWGDLGIDVVLESTGFFT
                     KRDDAAKHLSAGAKKVIISAPATDPDVTVALGVNFDDYDPENHNIISNASCTTNCLAP
                     FAKAINDAVGIEQGLMTTIHAYTSDQRLQDMPHKDLRRARAAAQNLIPTTTGAAKAVG
                     LVLPDLAGKLNGFAVRAPVITGSVVDLTFTPSKATSAEEVNAAVKAAAEGPLKGILAY
                     TEDPIVSTDIVGDPHSSIFDAALTQVIGDGTLVKAVSWYDNEWGYSNRCVELLGKVL"
     gene            3032..>3681
                     /locus_tag="PLJ64_11190"
     CDS             3032..>3681
                     /locus_tag="PLJ64_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNC94091.1"
                     /translation="MAFDKASVRDADVDGKRVLVRVDFNVPLDGEEVTDDTRIRAALP
                     TIEYLTGRGARVILCSHLGRPKGQVVPELSLAPAAKRLSELTGQPVGLADDVAGQDSE
                     AKAENLQPGQILLFENTRFEPGETENAPALAARLAALADIYVNDAFGAAHRAHASTAG
                     VAADLPAYAGLLLEKEVENLTSVATDPARPLVVVLGGAKVSDKVGVIDRFLEIADQ"
BASE COUNT          639 a         1287 c         1120 g          635 t
ORIGIN      
        1 ctggacgagc cgaccaccgg cttcgacccg tcggcccgcc ggcaggcctg ggagatggtg
       61 gccgggctcc gcggcctcgg caagaccgtg ctgctgacca cccattacat ggaggaggcc
      121 cagttcctcg ccgaccgggt ggcgatcatg agcgaagggc ggatcgtcgc cctcgacacc
      181 ccggagaacc tcggtgaccg ccgtcgcatg ccgaccacga tctccttctc ggtgccggcc
      241 ggaacggacc tggcgggcat cgccggagtc ggccagaccc agtcgagcag cgaagacgac
      301 accggcctcc gcacggtcag tatcggcact cacgagccgg tcgccgacct caaccggatt
      361 accggctggg caattgaaag cgacctcgag atccagcatc tcgaagtccg ccggcccagc
      421 ctcgaggatg tctacctgga gctgaccgac gcatgaagcc gatctccctg gcactgcacg
      481 agttccgctt tgcccagaag gtcttctggc gaaaccccgc ttcggtcttc ttcacggcgc
      541 tcctgccggt gctctttctg ctgatcttcg ccacgatctt tggcggcgac cagatcgagg
      601 aactgggggg agtcaaggtg accacctatt acatcccggc gatcgtcacc ctcgccgtga
      661 tctcggcgac catggtcaac ctgtcgatga acgtcacgat cgcccgggag cagggtctgc
      721 tcaagcgtgg ccgcggaacc ccgctgccgc cctgggtctt catcgccggc cgggtcggca
      781 acgcgatcgt gatctcggcc gtgatgctgg tcgtggtcac gctgatcggg aagctgctct
      841 atgacgcgcc gatcccctgg gaacggattc cggccgtgct cgtgaccctg gtcgtcggcg
      901 cgacttcgtt ctgctgcctc ggcctggcga tgaccgtgat cattccctcg cgcgaagccg
      961 cttcggcgat ctccaatgcg atcacccttc cgctctattt cctttccgga atcttcatcc
     1021 ccgaaaacga gatccccgaa ggtgtcctct cattcgccaa ccacttcccg gtgcgggatt
     1081 tcttcgaggc cttcttctcc gcctacagcc cgaagaccgt cggggcgggt ttcgagtggg
     1141 gcgaactcgc ggtggtcgcc atctggggcc tggccggatt cctcatcgcc ttgcgctact
     1201 tccgctggac cccccggcag tagtcgcccg gcgccgcaaa tccagggacc ggatttcacc
     1261 gttccgcccg gccgccctcg accactcgcc aacggtcagg ttcccgtttt gttgcatctc
     1321 cattagcctg ccgtgagtga ttcaccacat cgtcctcgaa gtttccgatc tccctcgcgc
     1381 ctcctcgttc tatgacgcgc tgctctcgcc actcgggtgg cgacgccacg tgaacgaaac
     1441 gaacgcggtc ggatacggca tttcccgtcc gatcctcttc atcaccgaac gaaccggagt
     1501 ccgggccgaa ggcacgatgg tcagcctcgg cgcccccggc atcgcggccg tcaaggccgc
     1561 ctgggaaggc ggaaccaact ccggcggctc gaacgtcgcc cagcccggcg aaaccagcgc
     1621 ccacggatcg ggctcctact ccgccttcct ccgcgaccct gacggtcacg acatagagat
     1681 caccgtctcc caggactaga ggtcccggcc tcgatccggg gcaggtgcct atggatttcg
     1741 ttcatataga cgaaatccat aggcacctcc cgaaactcgg gcctgaaccg gactcggagc
     1801 cgccacaggt ctacgggtga ccccacattc ccccgtcaat gccgctgttc accggcagtg
     1861 ggtcggataa ccggcagcag ggacggaaat ccgacccacc cctgatttcg gtgtttgacc
     1921 gcagggcacc gggggtggcg gggccgtaga atccaaggtg taatcggagt ttcaccaacc
     1981 cagttttccc aggaggcggt agcgaatgag cgtcaaggtg gcaatcaacg gttttggccg
     2041 gatcggccgg aacgttttcc gggcagcgca cgattccggt gctgatctgg acatcgtcgc
     2101 gatcaacgac ctggtcgaca ccgagaccct cgcgcacctg ctcaagtacg actcgatcct
     2161 cgggcgcctc gacgccgagg tgagcgccgg cgagggctcg atcaccgtcg acggcaagga
     2221 gatcaaggtc ctggccgagc gtgacccggc cgacctgccc tggggcgacc ttggaatcga
     2281 cgtggttctc gagtcgaccg gtttcttcac caagcgcgac gacgccgcca agcatctgtc
     2341 ggcaggcgcg aagaaggtca tcatctcggc gccggcgacc gatcccgacg tgaccgtggc
     2401 gctcggagtc aacttcgacg attacgaccc cgagaaccac aacatcatct cgaacgcatc
     2461 ctgcaccacc aactgcctgg cccccttcgc caaggcgatc aatgacgcgg tcggcatcga
     2521 gcagggcctg atgaccacga ttcacgccta cacctcggac cagcggcttc aggacatgcc
     2581 ccacaaggac ctgcgccggg cccgtgctgc cgcgcagaac ctgatcccga ccaccaccgg
     2641 tgcggccaag gccgtcggac tggtcctgcc cgacctggcc ggcaagctga acggcttcgc
     2701 ggttcgtgct ccggtgatca ccggttcggt cgtcgacctg accttcaccc cctcgaaggc
     2761 gaccagcgcc gaagaggtca acgccgcggt caaggccgcg gccgagggcc cgctcaaggg
     2821 catcctggcc tacaccgagg acccgatcgt ctcgaccgac atcgtcggtg acccccattc
     2881 gtcgatcttc gacgcagccc tgacccaggt gatcggtgac ggcaccctgg tcaaggcagt
     2941 ttcctggtac gacaacgagt ggggttactc gaaccgctgc gtcgagctgc tcggcaaggt
     3001 cctctaggac ggcttcagcg aggagctgga aatggctttc gacaaggcgt cggttcgcga
     3061 cgccgacgtc gacggcaagc gagtcctggt ccgggtcgac ttcaacgttc ccctcgacgg
     3121 ggaagaggtc accgatgaca cccggatcag ggctgcgctg ccaaccatcg agtatctgac
     3181 cggtcgcggg gccagggtca ttctctgttc ccacctgggc cggccaaagg gccaggtcgt
     3241 gccggagctt tcgctggcgc cggcggcgaa gcggctgtcg gagctgaccg gtcagccggt
     3301 cgggctggcc gatgacgtcg ctggccaaga ctccgaagca aaggccgaga accttcagcc
     3361 aggccagatc ctgctgtttg aaaacacccg cttcgagccg ggcgagaccg aaaacgcccc
     3421 ggcactcgcg gcccggctcg ccgcgctcgc tgacatctat gtgaacgacg ccttcggtgc
     3481 tgcccaccgg gcccacgcca gcaccgccgg cgtggccgca gacctgcccg cctacgcggg
     3541 cctgctgctg gagaaggaag tcgagaacct gacctcggtc gccaccgacc cggcccggcc
     3601 cctggtcgtg gtgctgggcg gggccaaggt ttccgacaag gtcggagtga tcgaccgctt
     3661 ccttgagatc gccgaccaga t
//
LOCUS       DAOQKN010000209         3680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_15380_length_3680_cov_7.721655, whole genome shotgun sequence.
ACCESSION   DAOQKN010000209 DAOQKN010000000
VERSION     DAOQKN010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15380_length_3680_cov_7.721655"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..934)
                     /locus_tag="PLJ64_11195"
     rRNA            complement(<1..934)
                     /locus_tag="PLJ64_11195"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(1359..2555)
                     /gene="tyrS"
                     /locus_tag="PLJ64_11200"
     CDS             complement(1359..2555)
                     /gene="tyrS"
                     /locus_tag="PLJ64_11200"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNC94092.1"
                     /translation="MSNVTQEPGEPSAEAVEQAAALTRTAVESLPEGRLARQLGLDRP
                     LRIKLGIDPTAPDIHLGHTVVLNKLREFQEQGHRVVLIIGDYTARVGDPSGRSDQRPM
                     LDPAEIDANAETFMEQAFKILDREKTEVRYNSEWLKMESDQLFALMARPTVARLLERD
                     DFQKRMAAEEPVSLLELVYPLLQGYDSVAIEADVEIGGTDQKFNLLFGRDMQSSFGQP
                     LQSIMTLPILPGIDGVKKMSKSTGNYIGVTDPPEEMFGKVMRVPDANLEEYWQLLLSE
                     DPPEGAGPNEAKRELGRRLVARFHGENAAPGAEEHFNRLFVRHEVPEEVEDFGIGDQV
                     HLPAALAEAFGVSRSEARRAITQGGVKLDGEQLPPEPLDYPSDQLDGKVLQLGKRRFR
                     RLVKGA"
     gene            2675..>3680
                     /locus_tag="PLJ64_11205"
     CDS             2675..>3680
                     /locus_tag="PLJ64_11205"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HNC94093.1"
                     /translation="MAPRKTEHPSGSTSRQNGRANGASGDAGAESAGGKALPESETKA
                     GSSESVLRPPGSGTRDRTAREASKGRTKGSRRIALPRLKGRKTKKAAGSGAAPTTAAS
                     GSATSRGKAATGNGNGQPLSIPSAPGVGGNGNGTGNGNGNGNGLPPGVLTLPVPPPRP
                     PKPKIKKLRVLFIVLGLGLLGFCSAFFGMVMAVSQDLPAIENYAQFRTAKNSILVDSQ
                     GKYIGTLSNNENQMLLDAEQISPNMKNAVVSIEDARFYEHRGIDFQGLGRALVQDIIA
                     RSAKQGASTITQQLVKQALEAQNDRSPLQKVREMSIAYHIEQQWTKDKILTEYLNTVY
                     F"
BASE COUNT          706 a         1214 c         1039 g          721 t
ORIGIN      
        1 gcccccgtca attcctttga gttttagcct tgcggccgta ctccccaggc ggggtactta
       61 gtgcgttagc tgcgatacgg aaggagtcga cacctcccac atctagtacc catcgtttac
      121 ggcgtggact accagggtat ctaatcctgt ttgctcccca cgctttcgca cctcagtgtc
      181 agttacgtcc cagcgagctg ccttcgctat tggtgttcct cctgatatct gcgcattcca
      241 ccgctacacc aggaattcca ctcgcctctt ccgtactcta gatcaacagt atccaccggc
      301 tttcgaaggt tgagccttcg actttcacag cagactaatt gatccaccta cgtgctcttt
      361 acgcccaatg attccggaca acgctcgctc cctacgtatt accgcggctg ctggcacgta
      421 gttagccgga gcttcttctg acggtaccgt caatcatctc ggctattcac cggacaacct
      481 tcgtcccatc tgaaagaggt ttacaaccct aaggccgtct tccctcacgc ggtgttgctg
      541 cgtcacgctt tcgcgcattg cgcaagattc cccactgctg cctcccgtag gagtctgggc
      601 cgtgtctcag tcccagtgtg gctgatcgtc ctctcagacc agctacccat cgtcgccttg
      661 gtaggccttt accccaccaa ctagctaatg ggccgcgggc ccctcccaat gcggaagccg
      721 aagctacctt tagtgaccag acttgtgtct gggcaccaca tccggtatta gcccgagttt
      781 cctcgggttg tcccggtcat cggggcagat tacccacgtg ttactcaccc gttcgcggct
      841 ttgccccagg ccgaagcctg gttcgtcgca ccacttgcat gtgttaagca caccgccagc
      901 gttcgtcctg agccaggatc aaactctccg tgaaaaatgt tgcgaggtat caaatgtcag
      961 tcgaaactga caaccggtcc gaagaccggg ttcctcaaac tatttactca acgtagagct
     1021 ttgtcatatc gggatgttgt cagtacacaa ttacgacaac ggatatgacg ggataattca
     1081 tgatcgtccg aagtcaaaga cctcgaacga atcgggctca tatgcttact gccagacgga
     1141 tgatccgtct ggagtaatgc acgctgctca gttttcaaag accgcttgcg ccgggcggcg
     1201 ggtttcgccg cacagagcac ttcagccggg caacctgctc gactgagcgg acggagaact
     1261 atacaggttt cccggagaag tgtcgagcgg attcgccgaa gtttcgcccg ggaattctct
     1321 tttggctctg ggaagccaaa tcccatcgca gagcgggatc aggcaccctt gaccagccgc
     1381 cggaaacggc gcttcccgag ctgcaggacc ttgccgtcaa gctggtccga ggggtagtcg
     1441 aggggctccg gcggcaactg ctcgccgtcg agcttcactc ccccctgggt gatcgccctt
     1501 ctggcctcgc tgcggctaac tccaaacgct tcggcgaggg ccgccggcag gtggacctgg
     1561 tccccgatcc cgaaatcttc cacttcctcg ggaacctcgt gccttacgaa tagccggttg
     1621 aagtgctctt cggcccccgg tgcggcgttc tcgccgtgga aacgcgcgac caggcgccgg
     1681 cccagttccc gcttggcctc gttcgggccc gccccctccg gcggatcctc acttaaaagg
     1741 agctgccagt actcctccag attcgcatcc ggcacccgca tcaccttgcc gaacatctcc
     1801 tccggcgggt cggtgacacc gatgtagttg ccggttgact tcgacatctt cttcaccccg
     1861 tcgattcccg gcaggatcgg cagggtcatg atcgactgca gcggctggcc gaaagacgac
     1921 tgcatgtccc ggccgaagag cagattgaac ttctggtcgg tgccgccgat ctccacatcg
     1981 gcctcgatcg ccaccgagtc gtagccctgg agcagtggat agaccagctc cagcagcgat
     2041 accggctcct ccgccgccat ccgcttctgg aagtcatccc tttccagcag ccgggcgacg
     2101 gtaggccggg ccatcagcgc gaacagctga tccgactcca tcttcagcca ctcgctgttg
     2161 tagcgaacct cggtcttctc gcggtcaaga atcttgaagg cctgttccat gaaggtctcg
     2221 gcgttcgcat cgatctccgc ggggtccagc atcggtcgct gatcggacct gccgctggga
     2281 tcaccgaccc gggccgtgta gtcgccgatg atcagcacga cccggtggcc ctgctcctga
     2341 aactcccgca acttgttcag gacgaccgtg tggccgaggt ggatgtccgg cgcggtcgga
     2401 tcgatcccca gtttgatcct gagcggccga tccagtccca gctggcgggc gagccggccc
     2461 tcgggcaggg actcaaccgc ggtccgggtc agcgccgcgg cctgctcgac tgcttctgct
     2521 gagggttcgc cgggttcttg agtgacgttt gacacgggag agtcaatgct agactgagca
     2581 gcgctacttg gtcgtgtgcg cgttgttcat gaaaggcgtg cagcgcatcc cacgacggct
     2641 ttcgccaagg cgctgaccta cttttcgcca tctgatggcc ccccgaaaga cagagcatcc
     2701 gtcgggatcg acttcccggc aaaacggacg cgccaacggt gcgtcaggtg acgcaggcgc
     2761 agaaagcgcc ggcggcaagg cgctgcccga atccgaaacg aaggcgggca gcagcgaaag
     2821 cgtgctccgg ccgccaggca gcggcacccg tgaccgcacg gccagggagg catcaaaggg
     2881 tcgcaccaaa ggttcccgcc ggatcgcact gcccaggctt aagggccgga agaccaaaaa
     2941 ggccgccggc tccggtgccg ccccgaccac ggccgcctcg gggtccgcga ccagcagagg
     3001 caaggccgcg accggaaacg gaaacgggca gcccctctcg atcccgagcg ctcccggggt
     3061 cggcggaaac gggaacggaa ccggcaatgg aaacgggaac ggaaacggcc tgccgcctgg
     3121 cgtgctcacc ctcccggtcc ctccgccccg gccgccgaaa ccgaagatca agaagctccg
     3181 ggtccttttc atcgtgctcg gtctcggcct gctcggtttc tgctcggcct tcttcggcat
     3241 ggtcatggcc gtttcgcagg atttgccggc gatcgagaac tacgcccagt tccggaccgc
     3301 caagaactcg attctcgtcg acagccaggg caagtacatc ggcaccctgt cgaacaatga
     3361 gaaccagatg ctgctcgacg cggagcagat ctcgccgaac atgaagaacg cggtggtctc
     3421 gatcgaggac gcgcgctttt acgaacaccg cggaatcgac tttcagggtc tcggacgggc
     3481 gctcgtacag gacatcatcg cccgcagcgc gaagcagggc gcctcgacca tcacccagca
     3541 gctcgtaaag caggcactcg aagcccagaa tgaccgctcg ccgctccaga aggtgcgcga
     3601 gatgtcaatc gcctaccaca tcgaacagca gtggaccaag gacaagatcc tcaccgagta
     3661 cctgaacacg gtctacttcg
//
LOCUS       DAOQKN010000210         3680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_15381_length_3680_cov_4.716414, whole genome shotgun sequence.
ACCESSION   DAOQKN010000210 DAOQKN010000000
VERSION     DAOQKN010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15381_length_3680_cov_4.716414"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(247..1968)
                     /locus_tag="PLJ64_11210"
     CDS             complement(247..1968)
                     /locus_tag="PLJ64_11210"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNC94094.1"
                     /translation="MHRSEDLTGEGRPDWLARGIGILPARIAFGVTMAVALFAALSFA
                     PSASRAASSSVPQEPPMILPAKAGASSAGSDGSSSDWIVGGVPGRVTSRIATASGGTA
                     IDRQLGSYRVSRDKARQLARRLRKADRLAYAEPDVPIERSGYPLDLFSDEQWWLNRIV
                     SPGDVTPPTVTARSPMIALIEESLDPLHPDLTSANLSGAKSLGPEADWHGTAVAGIIG
                     SPGEMLGIRGVWPGARMRLFASGLTCSTASKAVIKAVNAGAAVINMSYTFPADKCFTH
                     FKATQYAIRRNIVAVAAAGNTGDSGNAAVRPATDPHVISVGAVDKDTVVSSFSTRNSA
                     VDLTAPGEKVLAPYESDASVGGSTGVDRTWREVSGTSFSAPMVSAAAAWLRQVRPNLG
                     NLQINRLLTNSASDLGDPGRDPEYGAGLLSIEKSLSEPTPVQDPYEPNDDIRWINGSL
                     LGVKSPYLWRAGSGKRRMITATLSRAKDPADVYRLLIPARRQIIANVTQLEGDIVLTA
                     FKPKTRSIYKPGKNVIVRSNRAYPKTEGILVRNLKKRPQMIWLAITPSTSQTGNDSTY
                     RLKVARR"
     gene            2075..2734
                     /locus_tag="PLJ64_11215"
     CDS             2075..2734
                     /locus_tag="PLJ64_11215"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNC94095.1"
                     /translation="MAYPEAVLFDNDGLLLDTEAVWSRAEQRLFQERGMDFTIDHKRS
                     LVGSSAEIAGRKLARFLNEPGRDAEIMDELNDLVLAELEGGVDAMVGAREVIELLRLA
                     GVPIALVSNSPRRFIERSHEVAGTEMPFDAVVSGHEVPEAKPAPDAYLEACRQLGVEP
                     GKRVIVLEDSPSGVTAAVAAGLFVIGIPSVPGIDLSHADLIATSLESEPVLHALGVES
                     R"
     gene            complement(2785..3618)
                     /locus_tag="PLJ64_11220"
     CDS             complement(2785..3618)
                     /locus_tag="PLJ64_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94096.1"
                     /translation="MAGSLPLDDGRYLIRSGPAERVLVTETEGALPPPRRRRRRPRKS
                     GRTDAPVTVSVTVATVVLADQPLDGESAAEDWLDRLDDSDFTGDLIADAIGTLDRARA
                     ADAAASGAPFGTPTDPGSILAARIGYGEGEQVASGRFLESLDVDARGGTAESRRQRLA
                     RTAPLARTAAILGAREQSTACEVLIPRIRMDLETGNEAAARLAIAAAVGATISEMEFA
                     VEDPDHEKDLDRLEELVPGLAGVSERAGEGTSVAADTRLLEDALAVAERVIRRRRILG
                     Q"
BASE COUNT          571 a         1195 c         1310 g          604 t
ORIGIN      
        1 gaactgcgtc gagaacccac tttgcgccac ataccggcgc aaagtgggtt ctcggtgaac
       61 ccgcgcaagg gttcacagca acctgcggca ccggtcgcat cgattgtttc cgggtagccg
      121 cggggccggg tgagcgtgcc cgggatactc aggaagaggg cggctgaatg ccgcccgaag
      181 tgtttgccgc ttcccgggca cgctcacccg accccgcggc gtagccacca gggtccctca
      241 cgggagtcag cggcgggcga ccttgaggcg gtaggtggag tcgttgccgg tctggctggt
      301 cgagggggtg atcgcgagcc agatcatctg gggccgcttc ttgaggttgc ggaccaggat
      361 gccttcggtc ttcgggtagg cccggtttga gcggacgatc acgttcttgc cgggcttgta
      421 gatcgacctg gtcttcggct tgaatgcggt caggacgatg tccccttcga gctgggtcac
      481 gttggcgatg atctggcgtc gcgcggggat cagcagccgg tagacatcgg ccggatcctt
      541 ggccctcgag agggtcgcgg tgatcatccg gcgcttgccg gagcccgccc gccagagata
      601 gggggacttc accccaagca gcgaaccgtt gatccagcga atgtcatcgt tcggctcgta
      661 gggatcctgg accggagtcg gttccgaaag gctcttttcg atgctgagca gaccggcgcc
      721 gtattcgggg tccctgcccg ggtcaccgag gtcgctcgcc gagttggtca gcagccggtt
      781 gatctgaagg ttgccgagat tcggacgaac ctgacgcagc caggctgccg ccgccgaaac
      841 catgggtgcc gagaagctgg tgccgctgac ttcgcgccag gtccggtcca cgcccgtgga
      901 acctccgacg ctcgcatccg actcgtaggg cgccaggacc ttctccccgg gggcggtcag
      961 gtcaaccgcc gaattgcggg ttgaaaagga ggagacgacg gtgtccttgt ccacggcgcc
     1021 gaccgaaatc acgtgagggt cggttgccgg ccggacggcg gcgttgcccg agtcgcccgt
     1081 gttgccggcg gcggcgaccg caacgatgtt ccgccggatc gcgtactggg tcgccttgaa
     1141 atgggtgaag cacttgtcgg ccgggaaggt gtagctcatg ttgattacag cggcgccggc
     1201 gttgaccgcc ttgattaccg ccttcgaggc ggtcgagcag gtcagaccgg aagcaaagag
     1261 gcgcatcctc gcgccgggcc agacgccgcg aatgccgagc atctcgccgg gcgagccgat
     1321 gattcccgcc accgcggttc cgtgccagtc agcttcggga ccaagtgact tcgcgccgga
     1381 aaggttggcg ctggtcaggt cgggatggag cgggtccagc gactcctcga tcaaggcgat
     1441 catcgggctc ctcgcggtca ccgtcggggg cgtgacatcc ccggggctga cgatccggtt
     1501 gagccaccac tgctcgtcac tgaagaggtc cagcgggtac ccgcttcgct cgatcggcac
     1561 gtccggttcg gcgtaggcca gccggtcggc cttccgcagg cgacgggcga gctgcctggc
     1621 cttgtcgcgc gaaacccggt agctgccaag ctggcggtcg atcgcggtcc cccccgaggc
     1681 ggtcgcgatc cggctggtga cgcggccggg gacacctccg acgatccagt cgctgctgct
     1741 gccgtcactt ccggccgagc tggcaccggc cttggccggc aggatcatcg gcggttcctg
     1801 ggggacgctg ctggaggcgg cacgcgaagc cgagggcgcg aaggaaagcg cagcgaacag
     1861 cgcgaccgcc atagtcacgc caaaggcgat ccgggccggc aaaatgccga tcccccgggc
     1921 cagccagtcg ggccgacctt ccccagtcag atcttcggaa cgatgcatgc ctgtttgtaa
     1981 acccgcgaat cgcctcgtgg tatcggggca acacggaaat attccagacc ggggcggcgt
     2041 atccttgccg gcccgctgtg gctaggctcc gggcatggca tatccggaag ccgttctctt
     2101 tgacaatgac gggctcctgc tcgacaccga ggccgtgtgg agccgggccg agcagcgact
     2161 gttccaggag cggggaatgg acttcacgat cgaccacaaa cgcagcctgg ttggctcctc
     2221 ggctgagatc gccggtcgca aactcgcccg tttcctgaac gagccgggcc gcgacgccga
     2281 gatcatggac gagctgaacg acctggttct ggccgaactc gaaggtggcg tggatgcgat
     2341 ggtcggagcc cgggaagtga tcgagctgct gaggctggcc ggggtgccga tcgccctcgt
     2401 ttcgaactcg cctcgccggt tcatcgaacg ctcgcacgaa gtggcgggaa cggaaatgcc
     2461 cttcgacgcg gtggtcagcg ggcatgaggt gccggaagcg aagccggccc cggacgccta
     2521 tctcgaagcc tgccggcaac tgggagtgga gccgggcaag cgggtgatcg tgttggaaga
     2581 ctcgccttcc ggagtgaccg cggcggttgc ggcgggcctc tttgtgatcg gcatcccctc
     2641 ggtcccgggg atcgacctga gccacgcgga cctgatcgcc acctcgctcg agtcggaacc
     2701 ggtgctgcac gcgctcgggg tcgagtcgcg ctgatcccgc cccgggcctg aaccggcggc
     2761 cgccggtgct tgggttcctt ctgcctactg gccgagaatc cgccggcggc ggatcacccg
     2821 ctcggccacg gccagcgcat cctcgagcag cctggtgtca gccgccacgg aggttccctc
     2881 tccggcccgc tccgacaccc cggccaggcc gggaacgagt tcttccaggc ggtccagatc
     2941 cttctcgtgg tcgggatcct cgaccgcgaa ctccatctcg ctgatcgtgg ccccgaccgc
     3001 ggcggcgatc gccagcctgg cggccgcctc attgccggtt tcgaggtcca tcctgatccg
     3061 cgggatcagg acttcgcagg cggtggactg ttcccgggcc ccgaggatcg ctgcggtgcg
     3121 ggccagcgga gcggtgcggg caagccgctg cctgcggctc tcggcggtgc cgccgcgggc
     3181 gtcaacgtca agcgactcga gaaagcggcc cgaagcgacc tgctcgcctt cgccgtagcc
     3241 gattcgggcg gccaggatcg agcccgggtc ggtcggcgtt ccaaaggggg cgcccgaagc
     3301 cgccgcatcg gccgcccggg cgcgatcgag cgtgccgatg gcgtcggcga tcaggtcgcc
     3361 ggtgaaatcc gaatcgtcga gccggtccag ccagtcttcg gcagcggact cgccgtcgag
     3421 gggctggtcg gccagcacga cggtggccac cgtgaccgaa accgtgaccg gagcatcggt
     3481 ccggccggac ttgcgggggc gccggcgccg gcggcgggga ggaggcaggg cgccctcggt
     3541 ctcggtcacc agaacccttt cggcggggcc ggaacggatc aggtagcggc cgtcatcgag
     3601 cggcaggctg ccggccaatt cgaactggac gaagccgaaa agcgggctga tggcctagtt
     3661 gccttcgtcc gcggagcccg
//
LOCUS       DAOQKN010000211         3659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_15545_length_3659_cov_5.553274, whole genome shotgun sequence.
ACCESSION   DAOQKN010000211 DAOQKN010000000
VERSION     DAOQKN010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15545_length_3659_cov_5.553274"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PLJ64_11225"
     CDS             <1..229
                     /locus_tag="PLJ64_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94097.1"
                     /translation="AGRGSYRLVPGTAGDGLMLRAGPGVPDASGRFSQVPRIRRLELT
                     GTSGDLRFDFFRMKVRPWRSAPRAAVRLPTQ"
     gene            226..2211
                     /locus_tag="PLJ64_11230"
     CDS             226..2211
                     /locus_tag="PLJ64_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94098.1"
                     /translation="MNGFESKLRSWLEAIGRWNPPSWIPGLIIALLGWTVGFAHPFGG
                     LDSSWWVGLYLAAHNQMQFGTEIVFTYGPLGILRFPWLLYPGALAVIPFVYASILHVG
                     FSSALVAALRRRVGLLWAMLVALLVVVQLEWVEQEVAISILAAMLMIERRPAGRKLLF
                     FAVCAGLLAGTEMLVKLSTGPVIAAVLLVGLVGARARTREIAAYLGTLAGSMLVCWFV
                     TGQDLSNLPDFIGNSIQIVLGYQEAMAVYGQSGWYSLIIVAGGALCFAWALFGGYADR
                     RARIAGALVAAIVIFVFYKQAVVRIDSAHIALFFALIAVLWLAVPPRQGFAGISLAGL
                     AFMGVVSIYAATGVPSPGLNLISNLRGFYEESRTALSPARQRAGVDFWRQGIRHNYQL
                     DPKLLAELEGQSVSVEPWETTAAWAFELDWHPAPVFQNYSAYTSALDELNSDTIESAT
                     GPGRILRHVGDNPFGPTIGLDGRFMAWDPPGQAIAELCNFRPIGESGRWQVLARTANR
                     CGPLVPAGVVESRFGETVPVPKPGPNEVVIAKIHGAEVHGLERLRSFFYRPAERRAVV
                     EGGSYRLIAATAADGLMMRIGRNIPEGRGDFAQAPQTPTIALTGPSGDLKYEFFRFRV
                     RSAGPGLKDRPRREARLKRRAQLERRAGEAAQTVTAP"
     gene            complement(2160..>3659)
                     /locus_tag="PLJ64_11235"
     CDS             complement(2160..>3659)
                     /locus_tag="PLJ64_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94099.1"
                     /translation="LPHHDHPIMIPAALYDLSLSVFGMEPMPLHLVALALFLASVVML
                     FQWIRTLVGTPAAVLGCAVVLLLGSSTEDLIWTFQIGFCGSAVTGLGALLLLRRENTG
                     SDALACLLLVASFLLSTLAMPFLVGAAVQLCFRGGNPGLRRMLRDSWIVLVPLLLYGI
                     WLLGWNQSGGHAATLENALKTPFFVLSAFGYGSAALTGTFPLRLINDSYLWSIAGLAV
                     AAGFAWILQLRRRIPPEFLIALAAGLTFWILCGLNFTPGRDFFTGRYQYPSVIFILMM
                     LAGACAGLRPDRNWLKWLTVLAAASIAVNIFALFYTFNHTIKPFEQVNIVTLAAFKLS
                     GDAIDPDFQTPIGTGDDAPLTVPIYRDAVARYGPPAFTESDIDSATADYRSRLDQLLV
                     PALRIGPEDGLSVTPVRSTCETVTADAGATDTAPVAGPLLYIRPARDAVVRLTRFGPA
                     PGAPAWLVPGGRASGYRIPADDSDRPWRIGFQGQGAVTVCAASPARRSS"
BASE COUNT          587 a         1187 c         1305 g          580 t
ORIGIN      
        1 ggcggggcgc ggctcctacc gcctggttcc cgggaccgcc ggcgacgggc tgatgctccg
       61 ggccggaccc ggggtccccg acgcgtccgg gcgcttctcg caggttcccc ggattcgccg
      121 tctcgaactg accggcacct ccggcgatct caggttcgac ttcttccgga tgaaggtccg
      181 gccctggcgt tcggcgccgc gggcagcggt taggctgccg acccagtgaa cggattcgag
      241 tccaagcttc ggagctggct ggaggcgatc gggaggtgga atccgcccag ctggattccc
      301 gggctgatca ttgccctgct cggctggacg gtcggtttcg cacatccctt cggtggtctc
      361 gactcgagct ggtgggtcgg tctctacctc gcggcccaca accagatgca gttcgggacc
      421 gagatcgtct tcacctacgg tccgctcgga atcctccgtt tcccctggct gctctacccg
      481 ggcgcgctgg cggtgatccc gttcgtttac gcatcgatcc tgcatgtcgg tttcagctcg
      541 gccctggttg cggcactgag gagaagggtc ggtctgctct gggcgatgct ggtcgccctg
      601 ctcgtggtgg tccagctcga gtgggtcgag caggaggtgg cgatcagcat cctggcggcg
      661 atgctgatga tcgagcggcg cccggccggc cgcaagctgc tcttctttgc ggtctgtgcc
      721 ggattgctgg ccgggaccga aatgctggtc aagctttcga ccggcccggt gatcgccgcg
      781 gtcctcctgg tcggactggt cggggcgcgg gcgcggaccc gcgagatcgc ggcctacctc
      841 ggcaccctgg ccgggtcgat gctggtctgc tggttcgtga ccggccagga cctctcgaac
      901 ctgcccgact tcatcggcaa ctcgatccag atcgtgctcg gctatcagga ggcgatggcg
      961 gtctacggcc agtccggctg gtactcgctg atcatcgtcg ccggcggagc gctctgtttc
     1021 gcctgggccc tgttcggcgg ttacgccgac cgccgggccc ggatcgccgg tgccctggtc
     1081 gcggcgatcg tgatcttcgt cttctacaag caggcggtgg tgcggatcga cagcgcccac
     1141 atcgccctgt tcttcgccct gatcgccgtg ctctggctgg ccgtcccgcc gcgacagggg
     1201 ttcgccggaa tctcgctggc cggactggcc ttcatgggtg tggtttcgat ctatgcggcg
     1261 accggcgtgc cttcccccgg gctgaacctg atttccaacc tgaggggttt ctacgaggaa
     1321 agcaggacgg ccctcagccc ggcccgtcag cgggccggcg tcgacttctg gcgccaggga
     1381 atccggcaca actaccagct cgacccgaaa ctgctggccg agctcgaagg ccagagcgtc
     1441 agcgtcgagc cctgggagac caccgccgcc tgggccttcg agctcgattg gcacccggcc
     1501 ccggtcttcc agaactacag cgcctacacc tcggcgctgg atgagctgaa ttcagacacg
     1561 atcgagtcgg ccaccgggcc cggccgaatc ctgcggcatg tcggtgacaa tcccttcggg
     1621 ccgacgatcg gcctcgacgg aaggttcatg gcctgggacc cgcccggcca ggcgatcgcg
     1681 gaactctgca acttcaggcc gatcggcgag tcgggacgct ggcaggtgct ggcccgcacc
     1741 gccaaccgct gcggcccgct ggtcccggcc ggggtggtgg agtcccggtt cggtgaaacg
     1801 gtcccggtgc cgaagcccgg cccgaacgag gtagtgatcg cgaagatcca cggagccgag
     1861 gtccacgggc tggaaaggct gaggtcattc ttttaccggc cggccgagcg ccgggcggtg
     1921 gtcgagggag gctcctatcg cctgatcgcc gccaccgcgg cggacgggct gatgatgcgg
     1981 atcgggcgca acatccccga agggcgcggc gacttcgcgc aagccccgca gaccccgacc
     2041 atcgccctga ccggtccttc cggagacctg aagtacgagt tcttccgctt ccgggttcgc
     2101 tccgccggtc ccgggctgaa ggatcggccg aggcgcgaag cccggttgaa gcgccgggct
     2161 cagctggaac gccgggccgg tgaagccgcg cagaccgtaa ccgctccctg accctggaaa
     2221 ccgatccgcc agggccggtc cgaatcatcg gccgggatcc ggtagccgct cgcccgccct
     2281 ccgggcacca gccaggcggg cgctcccggg gcggggccga accgggtcag ccgcaccacc
     2341 gcatcgcggg cgggcctgat gtaaagcagg ggaccagcaa ccggcgccgt gtcggtcgcg
     2401 ccggcgtcgg cggtgaccgt ctcgcaggtt gagcggaccg gagttaccga aaggccgtcc
     2461 tcgggcccga tccggagggc cgggacgagc agctggtcga gtcgcgaccg gtaatccgcc
     2521 gtggccgaat cgatgtcgct ctcggtgaat gcgggaggtc cgtaccgggc gaccgcgtcc
     2581 cggtagatgg ggaccgtcag aggtgcgtca tcaccggtcc cgatcggggt ctggaagtcc
     2641 ggatcaatcg catcccccga cagcttgaag gcggccaggg tgacgatgtt gacctgctcg
     2701 aaaggcttga tcgtgtggtt gaaggtgtag aagagggcga agatgttgac ggcgatcgag
     2761 gctgccgcga gtacggtcag ccacttcagc cagttgcggt cgggccggag cccggcgcat
     2821 gccccggcca gcatcatcag gatgaagatc acgctcgggt actggtagcg gccggtaaag
     2881 aaatcccgtc cgggagtgaa gttgagcccg caaaggatcc agaaggtgag gcccgccgcc
     2941 agggcgatca ggaactcggg cgggatcctt cggcgaagct ggaggatcca ggcgaaaccg
     3001 gccgcaacgg cgagcccggc gatcgaccag aggtagctgt cattgatcag gcgcagcgga
     3061 aaggtgccgg tcagcgcggc cgagccgtag ccgaaggcgg agagcacgaa gaacggggtc
     3121 ttcagcgcgt tctcgagggt ggcggcgtgt ccgccggact ggttccagcc gagcagccag
     3181 atgccataga ggagaagcgg caccagcacg atccagctgt ccctgagcat gcggcgaagt
     3241 cccgggttac cgccgcggaa gcagagctgg acggccgcgc cgacgaggaa cggcatggcc
     3301 agggtggaga gcagaaagga ggcgaccagg agcaggcagg cgagggcgtc gctccccgtg
     3361 ttctcccggc ggagcagcag cagggcacca aggccggtga ccgcggaccc gcagaaaccg
     3421 atctggaagg tccagatcag gtcctcggtc gaggagccga gaagcagcac gaccgcgcag
     3481 ccgagcaccg cggccggggt cccgaccagg gttcggatcc actggaacag catgaccacg
     3541 ctggccagga agagagccag tgcgaccagg tgaagcggca tcggctccat cccgaagacc
     3601 gaaagcgaga ggtcgtacag cgccgccgga atcatgatcg ggtggtcgtg gtggggcag
//
LOCUS       DAOQKN010000212         3641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_15669_length_3641_cov_5.559955, whole genome shotgun sequence.
ACCESSION   DAOQKN010000212 DAOQKN010000000
VERSION     DAOQKN010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15669_length_3641_cov_5.559955"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..1370)
                     /locus_tag="PLJ64_11240"
     CDS             complement(51..1370)
                     /locus_tag="PLJ64_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HNC94100.1"
                     /translation="MAIGEEIFRPGSSSDGEVRAGILVTGTEVLTATIRDENGPWLSE
                     QLSGLGVELVEILVVADHPGDLARGLENMKSAGIDLIITTGGLGPTADDLTAEVVAAF
                     SGRKMELDEEMEARIAAILARFAANTNLDLSTDALNEANRKQAMIPVGAVALDPVGTA
                     PGLIVPGQAEGDPLVLVLPGPPRELKPMWEAAQKEPMLRQVLDQATRLEKYRLRMFGT
                     PESELAMSLREIEGEGLPFDGLEITTCLRKGEVEVDVRYREAAADAAEQLKQSLLERH
                     SRSLYSLDGSTVDEVVAGLLAGRRLGLAESCSAGLLAARIADVPGSSAYFAGGVVCYS
                     NEAKRDLLGVDQALLDEFGAVSAEVAEAMAVGALERFDADVSVAITGIAGPDGGTGDK
                     PVGLVHFNVRTAEGGVRAAAPIIPGGRRDVRERSALVAMHLLREILA"
     gene            complement(1428..>3641)
                     /locus_tag="PLJ64_11245"
     CDS             complement(1428..>3641)
                     /locus_tag="PLJ64_11245"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNC94101.1"
                     /translation="FAILAGILLLTGTTVAAVLGRSGKAIRKAGTTSAEKTRTFARTA
                     ILHDGKPEGADPAAKTVVDPVADPLPFGEEPTVTIGSGDSTDEMELPSKSSEEEEASP
                     FAVDIWAEDGGEPTISDKTEKLEPIEIFGEAGAHAAIGGPGTAVAEPGSDDGTGGGDG
                     QGEDSVFSDDFEEELKQLEEEFDEVPEEPDLQLETAPEPAAGAAAAGKEEQLKLTPQG
                     GKRGATLSEEINYKSPPLKVLEKGQKGGGPDPAEQKELARKLVETLGHFGVEAKVVGV
                     VTGPHVSRFELQLAPGTKVKKVSELANDLAYALASTDIRILAPIPGKQAVGVEVPNSS
                     RNMVRLGDIFGKPKEKDSPLVAWLGKGIDGKPVSTDLARMPHVLVAGTTGSGKSGCVN
                     AVLSSILMNSSPNDVRLVLVDPKQVELNHYETVPHLLTPVVTNPKLAANVLNNLIAEM
                     ETRYGLMKEARARNIEDYNRIVSAEGEPRLPYILCVIDELADLMMVAPADVEASIIRL
                     AQKSRATGIHLLLATQRPSTDIITGTIKVNIPSRIAFAVSSQTDSRVILDQGGAESLL
                     GQGDMLFRGPGTSKLARIQGAFVTEDEIARITDFWSAQGEPEFEQELLSEPETMQEET
                     SDAEFDPDQDDLLNEAIMLVVETETASVSMIQRRLRVGYTRAGRLIDMLERRGVISGY
                     EGSKPRQVLITHADLPRVLGGGNAGDVEGEPDAEASAGGEIADLPVPSETLDTSD"
BASE COUNT          554 a         1279 c         1149 g          659 t
ORIGIN      
        1 gggctggcgt gtgaaatttg gagggcgggt cggaccccgc ccgactcctg ttaagccaga
       61 atttcccgca ggaggtgcat ggcgaccaac gccgagcgct cgcggacgtc gcgacggccg
      121 ccggggatga tcggtgctgc cgcccgcacg ccgccctcgg cggtcctgac gttgaaatga
      181 accagcccga ccggtttgtc tccggttccg ccatccggac cggcgatgcc ggtgatcgcc
      241 accgacacat cggcgtcaaa tcgctcgagg gcaccgaccg ccatcgcttc cgcgacctcg
      301 gccgagaccg cgccgaactc gtcgagcaag gcctggtcga ccccgaggag atccctcttg
      361 gcctcgtttg aatagcaaac caccccgccg gcgaaataag cggaagagcc gggtacgtca
      421 gcgatccggg cggccagcaa accggcgctg caggattcgg ccagcccgag cctgcgaccg
      481 gccagcaggc ccgccacaac ctcgtcaacg gtgctcccgt cgagactgta gagcgacctc
      541 gaatgccgtt cgagcaacga ctgcttcagc tgctcggcgg cgtcggcggc cgcctcccgg
      601 tagcgcacgt caacctcgac ctcgcccttg cgcaggcagg tcgtgatttc caggccgtca
      661 aagggcagcc cctccccttc gatctcgcgc aggctcatcg ccagctccga ctcgggggtg
      721 ccgaacatgc gcaaccggta cttctccagc cgggtggcct ggtccagcac ctgccgcagc
      781 atcggctcct tctgtgccgc ttcccacatt ggcttgagct cccgcggcgg ccccggcagc
      841 accaggacca gcgggtcacc ttctgcctga ccgggaacga tcagcccggg tgcggttccg
      901 accgggtcga gggccaccgc tccgaccgga atcatcgcct gcttgcggtt ggcctcgttc
      961 agggcgtcgg tgctcaggtc aaggttcgtg ttggcggcga acctggcgag gattgccgcg
     1021 atccgcgcct ccatttcctc gtccagttcc atcttccggc cggagaaggc ggcgacgacc
     1081 tcggcggtga ggtcatcggc ggtcgggccg agaccaccgg tggtgatgat caggtcgatc
     1141 ccggccgatt tcatgttttc gagaccccgg gccaggtctc cgggatggtc ggcgacgacc
     1201 aggatctcga ccagctcgac ccccagcccg ctcagctgct ccgaaagcca gggcccgttc
     1261 tcgtcacgga tcgtggcggt cagcacctcg gtcccggtga ccaggatgcc ggccctgacc
     1321 tcaccgtccg agctgctccc cggcctgaat atctcctcgc caattgccac ggcctgaccc
     1381 tacagccgcg gcaccggttg cggcctccgc cgaagcccgg cttccggcta atccgaggtg
     1441 tcgagcgtct cggacgggac cggcaggtcg gcaatttcgc cgcctgcgga agcttcagcg
     1501 tcaggttccc cctccacatc gccggcattt ccgccgccaa gcacccgcgg cagatcagcg
     1561 tgggtgatca gcacctggcg gggcttcgaa ccttcgtaac cggagatcac gccccggcgc
     1621 tcgagcatgt cgatcaggcg cccggcccgg gtgtaaccga ccctcagacg cctttgaatc
     1681 atcgataccg aagccgtctc ggtctcgacc accagcatga tcgcctcgtt cagcaggtcg
     1741 tcctggtccg gatcgaattc cgcgtccgaa gtctcttcct gcatcgtctc cggctcgctg
     1801 agcagttcct gttcgaattc cggctcgccc tgggccgacc agaagtcggt gatccgggca
     1861 atctcgtcct cggtcacgaa ggccccctgg atgcgggcca gcttcgaggt accggggccg
     1921 cggaagagca tgtcgccctg gccgagcagc gactcggcac cgccctggtc gaggatcacc
     1981 cgcgagtcgg tctgcgaaga gaccgcgaag gcgatccggg agggaatgtt caccttgatc
     2041 gtgccggtga tgatgtcggt cgacggccgc tgcgtggcca gcagcaggtg gatgccggtc
     2101 gcccgcgact tctgggccag ccggatgatc gaggcctcga catcggccgg ggcgaccatc
     2161 atcaggtcgg ccagttcgtc gatcacgcag aggatgtagg gcagtcgcgg ctcgccctcg
     2221 gccgaaacga tccggttgta atcctcgatg ttgcgggccc gggcctcctt catcaggccg
     2281 taacgggttt ccatctcggc gatcaggttg ttcagcacgt tggcggccag cttcggattg
     2341 gtcaccaccg gggtcagcag atgcggaacc gtctcgtagt gattcagctc gacctgcttc
     2401 gggtcgacca gcaccagccg cacgtcattc ggcgaggaat tcatcaggat cgacgagaga
     2461 accgcgttca cgcagccgga cttgcccgaa ccggtggtgc cggcgaccag cacatgcggc
     2521 atcctggcca ggtcggttga aaccggcttg ccgtcgattc ccttgcccag ccaggcgacc
     2581 agcggcgagt ccttctcctt gggcttgccg aagatgtccc ccagccggac catgttgcga
     2641 ctcgagttgg gaacctcgac cccgaccgcc tgcttgcccg ggatcggcgc cagaatgcgg
     2701 atgtcggtcg aagcgagcgc gtaggcgagg tcattggcca gttccgagac cttcttgacc
     2761 ttggtgcccg gcgccagctg aagctcgaag cgggaaacgt gaggcccggt caccaccccg
     2821 accaccttcg cttcgacccc gaagtgaccg agggtctcga ccagcttgcg ggccagttcc
     2881 ttctgctcgg cgggatccgg tcctccgccc ttctggccct tctccagcac cttgagcggc
     2941 ggggacttgt agttgatctc ttccgaaagc gtcgccccgc gcttgccgcc ctgcggggtc
     3001 agtttgagct gctcctcctt gcccgcggca gcggcccctg cggccggctc cggcgcggtt
     3061 tcgagctgca ggtccggctc ttccggcact tcgtcgaact cctcctcgag ctgcttcagc
     3121 tcttcttcga agtcatcgct gaagaccgag tcctcgcctt gaccgtcacc gccgccggtg
     3181 ccgtcgtcgg agcccggctc ggccacggcg gtacctggtc cgccgattgc cgcatgtgcc
     3241 ccggcttcgc cgaagatctc gatcggctcc agcttctcgg tcttgtcgga gatcgtcggc
     3301 tcgccgccgt cctcagccca gatgtcgacc gcgaagggcg aagcttcctc ttcctccgat
     3361 gatttcgaag gcagttccat ctcgtcagtc gaatcgccgc tcccgatggt cacggtgggc
     3421 tcttccccga aggggagcgg gtccgcaacc gggtcgacga cggtcttggc cgcggggtcc
     3481 gctccctcgg gcttgccgtc gtggagaatt gcggtccggg caaaggtgcg ggtcttctcg
     3541 gccgatgtcg tgccggcctt cctgatcgcc tttccgcttc gcccgagaac ggccgcaacc
     3601 gtggtcccgg tcagcaagag aatcccggcg aggatcgcga a
//
LOCUS       DAOQKN010000213         3610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_15909_length_3610_cov_3.985654, whole genome shotgun sequence.
ACCESSION   DAOQKN010000213 DAOQKN010000000
VERSION     DAOQKN010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15909_length_3610_cov_3.985654"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1020)
                     /locus_tag="PLJ64_11250"
     CDS             complement(<1..1020)
                     /locus_tag="PLJ64_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94102.1"
                     /translation="MRLFHRQISILVAIATVFCFALTTTAFAEDTTQPEPFTLISPVD
                     NAVNQRNETLLSWNEADDLDSGIDHYELRVDDATVEEIDAVDACFQGVCRMWQESGGN
                     LRNWQIRAVDHNGNSRDSEVRTYSLDRDPPNEFQLTSSGILTDTNAVLTWDEASDPGV
                     GVAGYQIFIDGSRIFESGQCIDGECSVSFLSAPGHHTWFIKAFDWNGNFRDSDSSDFT
                     ATVGTSLQTENTSVSVQHKSTRTDLGFTVSPVGPGQVSFECSLDGGPWTGCGGTISSG
                     VLQSQLTGLSDGSHTLAARSIHPEFGADPSPGTLTWTVDTQSPPTASLSPEPSIASTG
                     QQVILD"
     gene            complement(1112..1193)
                     /locus_tag="PLJ64_11255"
     tRNA            complement(1112..1193)
                     /locus_tag="PLJ64_11255"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1157..1159),aa:Tyr,seq:gta)
     gene            1332..2393
                     /locus_tag="PLJ64_11260"
     CDS             1332..2393
                     /locus_tag="PLJ64_11260"
                     /inference="COORDINATES: protein motif:HMM:NF013384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HNC94103.1"
                     /translation="MTDPALRGFASDNSSGAHPAMLEAIARVNGGHVGSYGADPETAR
                     FRELVKAEFGESATGFPMLNGTGANVAALRAMARPHQAVICSSTAHIHIDETAAPEAI
                     AGLKLLTVDTADGKLTPELAATRLADPADVPHAAWPVVISVAQATEVGTVYTPDEIRA
                     LAEFAHERGMLLHIDGARLANAAASQDLSLNEASLGAGADVLSLGANKNGAVFGESVI
                     FADPELARGFEVLRKGSLQLASKMRFVSAQLCAQLEDGLWREIAGHSNSMARLLADGI
                     EWTEGADLGQPVQANIVFLSLPIEPARKLVEACAPHQPLLFEAGEAGIIRLVCSWDTE
                     PGDVDQFLGLLRDVLPAPS"
     gene            complement(2472..3131)
                     /locus_tag="PLJ64_11265"
     CDS             complement(2472..3131)
                     /locus_tag="PLJ64_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC94104.1"
                     /translation="MSKRVKVAIVDDHAIFRAGVRSELVAGVEVVGEADGVADAGLLL
                     ARTSPQVVLLDVHMPEGGGVEVIRRAAAKAAESGEPPPRFLALSVSDDPEDVVSVIRA
                     GARGYVTKSISGPDLIDAIERVDQGDAVFSPQLAGFVLDAFAGATAAEQDDELDLLTP
                     REQEVLRLIARGYMYKEIARRLGISVKTVEAHTSSTLRKLQLSTRHELARWATERNLL
                     D"
     gene            complement(3128..>3610)
                     /locus_tag="PLJ64_11270"
     CDS             complement(3128..>3610)
                     /locus_tag="PLJ64_11270"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC94105.1"
                     /translation="NADDSRQVSTLARSQERELRDWLSGKTDRDRADSLAESLKAVAA
                     EVEERHQVPIDAVTVGDRPLDENSIALVAASREAMTNAARHAGQAGPVRLYAEIDPTR
                     SEVFIHDRGPGFDPEAVPADRRGVRDSILGRMEHHGGHAEIRSTPGEGTEVELVMETK
                     "
BASE COUNT          581 a         1149 c         1253 g          627 t
ORIGIN      
        1 gtcaaggatc acctgctgcc cggtcgacgc gatcgagggt tcggggctga ggctggcggt
       61 tggcggactc tgggtatcga ccgtccaggt caaggtgccg gggctagggt cggccccgaa
      121 ctcagggtgg atcgagcggg cagccagggt gtgggaaccg tcggagagcc cggtcagctg
      181 ggactggagc actcccgagg agatcgtccc gccgcatccg gtccaggggc ctccgtccag
      241 gctgcattca aaactgacct ggccgggccc gacaggcgag acggtgaatc ccaggtcagt
      301 gcgggtggat ttgtgctgaa ccgagaccga agtgttttcg gtttgcagac tggtgccaac
      361 cgtggccgtg aaatccgagg aatctgaatc ccggaagttg ccgttccagt caaacgcctt
      421 gatgaaccag gtgtgatgac ccggggcgga aaggaagctg accgaacact ctccatcgat
      481 gcattggccg gattcgaaaa tgcgactgcc gtcgatgaag atctggtagc cagccacccc
      541 gacccccgga tcggacgcct cgtcccaggt cagaacggca ttggtgtcgg tgagaatgcc
      601 ggacgaggtc agctggaact cgtttggcgg gtcccggtcg aggctgtagg tgcggacttc
      661 ggaatcccgt gaattgccgt tgtgatcgac cgcccggatc tgccagttgc ggagattgcc
      721 gccgctttcc tgccacatcc tgcacactcc ctggaaacag gcgtccacag cgtcaatctc
      781 ttcgacagtg gcatcgtcga cccggagttc gtagtggtcg ataccggagt cgaggtcatc
      841 ggcttcgttc caggacagca gagtctcatt gcgctggttg acggcgttgt cgaccggact
      901 gatcagggtg aaaggttcgg gctgcgtcgt gtcttcggcg aaggcggtgg tggtcagcgc
      961 gaagcagaag acggtcgcga ttgcgacgag tatcgatatc tggcggtgaa acagacgcaa
     1021 gttgactccc tgttcctcgt gtcctttatt cagactagcg cccggccggg gcctgaccaa
     1081 aaaagccggc cgcggtgggc ggccggctgg atgggggaga caggattcga acctgtgtag
     1141 gcagagccaa ctggtttaca gccagtcccc tttaaccact cgggcactcc cccggctgag
     1201 ccggtcagtt tattgacccg gccatttcgg tggctcgcgg gcggcccgaa cccccggcct
     1261 cacggggtgg gtcggatttc cagcggagct gcgggttatc cgacccactg catccgggca
     1321 tacttcgagg catgactgat cctgccttgc gggggtttgc ctccgacaac agctccggtg
     1381 cccacccggc gatgcttgag gcgatcgccc gggtaaacgg cgggcatgtg ggctcctacg
     1441 gggcagatcc ggagaccgca agattcaggg aactggtgaa ggccgagttc ggcgaaagcg
     1501 cgaccggctt tccgatgctg aacgggaccg gggcgaacgt cgccgcgctc cgggcgatgg
     1561 cccggccgca tcaggcggtg atctgctcct cgaccgccca catccacatc gacgagaccg
     1621 cggcgcccga ggcgatcgcc ggactcaagc tgctgaccgt ggacaccgcc gatggcaagc
     1681 tgaccccgga gctggccgcg acccgcctgg ctgacccggc cgacgtgccc cacgccgcct
     1741 ggccggtcgt gatctcggtc gcccaggcca ccgaggtcgg caccgtctac acccctgacg
     1801 agatccgggc cctggccgaa ttcgcccacg aacggggcat gctgctccac atcgacgggg
     1861 cacggctggc caatgccgcc gcgtcgcagg acctctcgct gaacgaagcc agcctcgggg
     1921 cgggggcgga tgtgctgagc ctgggggcga acaagaacgg ggcggttttc ggcgagtcgg
     1981 tgatcttcgc cgaccctgaa ctggctcgag gcttcgaagt gctccgtaag ggctcgcttc
     2041 agcttgcctc gaagatgcgc ttcgtctcgg cccagctatg cgcccagctc gaggacggtc
     2101 tctggcggga aatcgccggt cactcgaact cgatggcacg gctgcttgcc gacgggatcg
     2161 aatggaccga aggcgccgac ctcggtcagc cggttcaggc caacatcgtc tttctctcgc
     2221 tgccgatcga gccggcccgg aagctggtcg aggcatgcgc accgcatcag ccgctcctct
     2281 tcgaggcggg cgaggcgggg atcattcgtc tggtctgttc ctgggacacc gagcccgggg
     2341 acgtggacca gttcctcggt cttctgcggg acgttcttcc cgcgccgagc tagcgccgag
     2401 ctagcgccga gtgcccactt tgtgccggta tgtggcgcaa agtggacact cgatgagatc
     2461 cgtagacccg atcagtcgag caggtttcgc tcggtggccc agcgggcgag ttcgtgacgg
     2521 gttgagagct ggagcttgcg cagggtggag gaggtgtggg cctcgaccgt cttgaccgag
     2581 atgccgagcc ggcgggcgat ctccttgtac atgtagccgc gggcgatcag gcgcaggact
     2641 tcctgttcgc gcggggtgag caggtcgagt tcgtcgtcct gttcggccgc ggtggctccg
     2701 gcgaaggcgt cgagcacgaa gccggccagc tgcggcgaga agacggcgtc accctggtcc
     2761 acccgctcga tcgcgtcgat caggtccggg ccggagatcg acttggtcac gtatccgcgg
     2821 gccccggccc ggatcaccga gaccacgtcc tcgggatcgt cggaaaccga gagagcgagg
     2881 aagcggggcg ggggctcgcc gctttcggcc gccttggccg cggcccggcg gatcacttcg
     2941 accccgccgc cctccggcat gtgaacgtcg agcaggacca cctgggggga ggtgcgggcc
     3001 agcaggagac cagcgtcggc gacgccgtcg gcctctccga ccacctcgac cccggccacc
     3061 agttcggagc ggaccccggc ccggaagatc gcgtggtcgt cgacgatcgc gaccttgacc
     3121 cttttgctca tttcgtctcc atcacgagtt cgacttcggt cccctcgccg ggggttgacc
     3181 ggatctccgc gtggccgccg tggtgttcca tccttccgag gatcgagtct cgcaccccgc
     3241 gccggtcggc ggggaccgcc tccgggtcaa agccggggcc gcggtcgtga atgaaaacct
     3301 cggaccgcgt cgggtcgatc tcggcgtaga gacgaaccgg acccgcctgg ccggcgtggc
     3361 gggcggcgtt ggtcatcgcc tcgcgactgg cggcgaccag ggcgatcgag ttctcgtcga
     3421 ggggccggtc accgacggtg accgcatcga tcggcacctg atgcctctcc tcgacttcgg
     3481 cggcgaccgc cttcagcgac tcggccagcg agtcggcccg gtcgcggtcg gtcttgcccg
     3541 agagccagtc gcgcagttcc cgctcctgcg agcgggcgag ggtcgagacc tgacgggagt
     3601 cgtcggcgtt
//
LOCUS       DAOQKN010000214         3595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_16020_length_3595_cov_4.688136, whole genome shotgun sequence.
ACCESSION   DAOQKN010000214 DAOQKN010000000
VERSION     DAOQKN010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16020_length_3595_cov_4.688136"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="PLJ64_11275"
     CDS             complement(<1..677)
                     /locus_tag="PLJ64_11275"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNC94106.1"
                     /translation="MSDSGLDPQRIASLTEAQQEIYRNRTGKSAAEYQRAVKVMPGGV
                     PSSFQMMDPWPVYLTEGKGSQVWDVDGNQYTDFHAGFGVMVIGHANPTIGEAVKARID
                     QSTHFAAPTNDSIEVAEELKKRFGLPHWRFINSGTEATMDAVHLARHQGGHAGAGAGD
                     EAQFGALPRLRLAEPGGIAFHHHALARREGDEPVGAGADGAGGEVGRAHVGVQPLAQQ
                     HDQRQFAV"
     gene            complement(815..2266)
                     /locus_tag="PLJ64_11280"
     CDS             complement(815..2266)
                     /locus_tag="PLJ64_11280"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNC94107.1"
                     /translation="MNFIDDVIQKFPFSQEAVIAIDGEGNRTVFCFSHLFARSLGLSG
                     AMLDRGVRRGDVVMILVGSRIEWVVSMLACFRMGAIALPLNPQLSEHDLAYRVAATNP
                     ALAIGEADYLAGLPDGIPAFDLEDLDRIFDEDLAQATPAKVASLALDDPALIVFTSGT
                     TAEPKGVVHSQRYLMGQAVQAQHWVGAEPDDLCWCTAAPGWSKSSRNSFIAPWLRGAK
                     SLLVEGRFDPATRMDIVRQQEVNVLCQAPTEYRILAKRTTLSPAPSLRRMVSAGEALN
                     PEVIGAFREATGLEIADGYGQTETGAVTGMRPGEAVAGREGSMGRPLPGIETRIVEGE
                     LQVMAATSPTFFHSYIDGSRFEGEWWATGDLVREDDEGYLFHEGRNDDVISSAGYRIG
                     PVEVESALLSHPAVAEAAAIASPDPERGSVVKAVVVLRDRHGDEALAAELQEHCRRET
                     APYKYPRLIEFTDELPKTASGKIRRAELRDRET"
     gene            complement(2564..2800)
                     /locus_tag="PLJ64_11285"
     CDS             complement(2564..2800)
                     /locus_tag="PLJ64_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94108.1"
                     /translation="MSNETMDSVFIDIELDEPAASDPELAKKLEEVCTVGIFKATENG
                     TEIVRGQLDECVLCYLCEEAAPEGSLRIIKKYEN"
     gene            complement(2797..>3595)
                     /locus_tag="PLJ64_11290"
     CDS             complement(2797..>3595)
                     /locus_tag="PLJ64_11290"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNC94109.1"
                     /translation="VTPGRKGREVHGVPVFDTVEQAVENHGSQIDGSIVTVPPGFTKD
                     AVIEAIDNGIKLIVVVTERIPRGDVAEMVEFANMHDARIIGPNCLGLIVPEVCKMGGI
                     GGPAKDAAKSYSPGVVGVMSRSGGMTTEISSSLTAAGLGVSTAVSIGGDAIIGSSYAE
                     LMPYFEADEETRAIVIYTEPGGRMEAQLADWQAENNSRLPIVAFMAGKFMDDESMKGM
                     TFGHAGTIVEGKEDSATEKIKRLESAGITVVERIDEIPDAVKERIGA"
BASE COUNT          568 a         1180 c         1187 g          660 t
ORIGIN      
        1 accgcgaact gtcgctgatc atgctgctga gcgagcggct gcacgccgac atgcgcacgc
       61 ccgacttcgc cgcccgcgcc atcggcaccg gcccctacag gctcgtctcc ttcgcgccgg
      121 gcgagcgcat ggtgatggaa cgcaatcccg cctggttccg cgaggcgcag ccgtggcagc
      181 gcgccgaact gcgcttcgtc accagcgccg gcgcccgcat ggccaccctg atggcgggcg
      241 aggtggacgg catccatggt cgcctcggtc cccgagttga tgaaacgcca atggggaagc
      301 ccgaagcgct tcttcagttc ttccgccact tcgatcgagt cgttggtcgg ggcggcaaag
      361 tgggtgctct ggtcgatccg ggccttgacc gcctcgccga tcgtcggatt cgcgtggccg
      421 atcaccatca cgccgaaacc ggcgtggaag tccgtgtact ggttgccgtc gacgtcccag
      481 acctgggatc ccttgccctc ggtcaggtag accggccagg ggtccatcat ctggaaggag
      541 gaaggcactc cccccggcat cactttcacc gcgcgctggt attccgcggc ggacttgccg
      601 gtgcgattgc gatagatctc ctgctgcgcc tcggtcagtg aggctattcg ctgggggtca
      661 aggccgctgt cagacatctg ctctcctcgg tcgtggggta gggatcagcc tatatatctg
      721 cccgccgccg atccagactc aaacgtcaga ggttccggac cgggctggac attttgacaa
      781 gcagagccga cccggccatc ttcaggtctt gcggtcaggt ttccctgtcc ctgagttctg
      841 cccttcggat cttgccggag gccgttttcg ggagctcgtc ggtgaactcg atcagccggg
      901 ggtacttgta gggcgcggtt tcccggcggc agtgttcctg cagttcggcg gccagggcct
      961 cgtcaccgtg gcggtcgcgc agcaccacca cggctttcac gaccgaaccg cgttccgggt
     1021 cgggcgaggc gattgccgcc gcctcggcca ccgcgggatg cgagagcagc gccgattcca
     1081 cctcgaccgg accgatccgg tagccggccg aggagatcac gtcatcgttc cggccttcgt
     1141 gaaagagata accctcatcg tcctcgcgga ccaggtcgcc ggtcgcccac cactcgcctt
     1201 cgaagcggct tccgtcgatg tagctgtgga agaaggtggg ggaggtggct gccatcacct
     1261 ggagttcgcc ctcgacgatc cgggtctcga taccgggcag aggccggccc atcgaccctt
     1321 cgcgaccggc cacggcctcg ccgggccgca tgccggtcac ggcgccggtc tcggtctggc
     1381 cgtaaccgtc ggcgatctcg agtccggtcg cctcgcggaa ggcaccgatc acttcggggt
     1441 tcagcgcctc gccggccgag accatcctcc gtagggaggg ggcgggggag agagtggttc
     1501 gcttggccag gatccggtac tcggtcgggg cctggcagag cacgttgacc tcctgctggc
     1561 ggacgatgtc catccttgtg gcggggtcga aacgaccctc gaccagcagc gacttcgctc
     1621 ccctcagcca gggggcgatg aaggagtttc gcgaagactt cgaccagccc ggggcggcgg
     1681 tgcaccagca aaggtcgtcc ggctcggccc cgacccagtg ctgggcctga acggcctggc
     1741 ccatcagata gcgctgcgag tggactacgc ccttcggttc ggcggtggtg ccggaggtga
     1801 agacgatcag ggccgggtca tccagagcca ggctggcaac cttggccggc gtcgcctggg
     1861 cgaggtcctc gtcaaagatc cggtcgaggt cttccagatc gaaggcgggt atcccgtccg
     1921 gcaggccggc caggtagtcg gcctcgccga tcgccagagc aggattggtg gcggcgaccc
     1981 ggtaggcgag gtcgtgctcg ctgagctggg ggttgagcgg cagcgcaatc gcccccatcc
     2041 ggaagcaggc cagcatcgaa acgacccact cgatcctgct tccgaccagg atcatcacga
     2101 catccccgcg ccgcactccc cggtccagca tcgcccccga aaggcccaga ctccgcgcaa
     2161 aaaggtgaga aaaacagaag accgtccggt tcccctcacc gtcaatcgca ataacggcct
     2221 cctgcgaaaa agggaacttc tggattacgt catcaatgaa gttcaaggtc cgccgagtct
     2281 agccagcctt ggcgcttgcg tgaccgggtc cggtcagtgc gccccgacga gtttcgggtc
     2341 ggacaatcga gatgcccggg cgacctggtt gcgggagtgc ccgccgacga cgtttcgggc
     2401 caccctttcg aacgagtttg gttgagcggg tccgggcagt gcgccccgac gacgtttcgg
     2461 gccacccttt cgaacgagtt tggttgagcg ggtccgggca gtgcgccccg acgacgtttc
     2521 gggccgagga ggggtgtact gcccggaccc gctctaacca gagctagttc tcgtacttct
     2581 tgatgatccg aagcgacccc tcgggagcgg cttcttcgca gaggtagcag aggacgcact
     2641 cgtcgagctg gccgcgaacg atctcggtgc cgttctcggt cgccttgaag atgcccacgg
     2701 tgcagacctc ttccagcttc ttggccagct cgggatccga ggcggcgggc tcgtcgagct
     2761 cgatgtcgat gaagacgctg tccatcgttt cgttgctcat gctccgatcc tttccttgac
     2821 tgcgtcgggg atctcgtcga tccgctcgac cacggtgatg ccggccgatt ccagacgctt
     2881 gatcttctcg gtcgccgagt cttccttgcc ctcgacgatg gtgccggcat ggccgaaggt
     2941 catgcccttc atcgactcat cgtccatgaa cttgccggcc atgaaagcga cgatcggcag
     3001 gcgggaattg ttctcggcct gccagtcggc gagctgggct tccatgcggc cacccggctc
     3061 ggtgtagatg acgatcgccc tggtttcctc atcagcctcg aagtacggca taagctcggc
     3121 gtagctcgag ccgatgatcg cgtcgcctcc gatcgagacg gcggtcgaga cgcccaggcc
     3181 ggcggccgtg agcgaggagg agatctcggt ggtcatgccg ccggagcggg acatcacgcc
     3241 gacgacaccg gggctgtacg acttggcggc gtccttggcc gggccgccga tgccgcccat
     3301 cttgcatacc tcggggacga tcaggccgag gcagttcgga ccgatgatcc gggcgtcatg
     3361 catgttggcg aactcgacca tctcggccac gtcgccgcgc gggatgcgtt cggtgacgac
     3421 gacgatcagc ttgatcccgt tgtcgatcgc ctcgatcacc gcgtccttgg tgaagccggg
     3481 ggggacggtg acgatcgagc cgtcgatctg gctgccatgg ttttcaaccg cctgctcgac
     3541 cgtgtcgaag accggcacgc cgtggacttc gcggcccttg cggccggggg tcacg
//
LOCUS       DAOQKN010000215         3586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_16091_length_3586_cov_5.433871, whole genome shotgun sequence.
ACCESSION   DAOQKN010000215 DAOQKN010000000
VERSION     DAOQKN010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16091_length_3586_cov_5.433871"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="PLJ64_11295"
     CDS             <1..218
                     /locus_tag="PLJ64_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNC94110.1"
                     /translation="FWVTGDSFMQNMVRILVGTMLEVADGSRSLDNFVQLLEGAERPD
                     AGVTAPPQGLYFVRVDYPDPPPLPVAR"
     gene            270..1079
                     /gene="surE"
                     /locus_tag="PLJ64_11300"
     CDS             270..1079
                     /gene="surE"
                     /locus_tag="PLJ64_11300"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNC94111.1"
                     /translation="MRILLTNDDGIGAKGLQTLRRELRQIEGVTLDVIAPDSNRSASA
                     RSITTRTPLSVEEIHFDDGDLGHATDGTPVDCVRFAQVGLLGEKPDLIVSGINHGLNL
                     GDDVTYSGTVAAALEGILLGLPAVAVSQQSEKGEMGYMPQRHYEFDTVAKFTAGLVRE
                     MLDHPLPEDTLLNINAPGGEVQGVDVTKLGKRLYRDELKKVERDETNGRTSYRIYGYE
                     PGLDDEPGTDIGAIARGRLTVTPLSLDWTHHDEIEGLRGRDFERLLGAATS"
     gene            1076..3151
                     /gene="ligA"
                     /locus_tag="PLJ64_11305"
     CDS             1076..3151
                     /gene="ligA"
                     /locus_tag="PLJ64_11305"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNC94112.1"
                     /translation="MSEEEAAAGEPSPDDASRIAELREVLNHHIVLYYEKDDPEISDE
                     EYDRLFAELQELEGRNPELITGDSPTQRVGAPASTSFAPVRHAVPMLSLANARTEEEL
                     TAWENRIRNHLLRRDVDPGEITFVTEPKIDGLAISLTYENGRLTKGATRGDGQVGEDV
                     TLNIETIRTVPKQIDDAPPLIEVRGEVYLPIADFAELNQKRVDAGLPAFANPRNSAAG
                     SIRQNDPKAAAERPLAVWSYGIGAMDGIRFASHSEEIEWLRDHGFHVNPDIAVHDSIG
                     EVLDRCHWWLDRREDLDFEIDGVVVKIDDIGLWNELGNAGREPRWAIAWKFPPTTATT
                     KLLEIEWNVGRTGHLVPWAKLDPVRVGGVTVSNATLHNEEDLERKDVRAGDEVVVLRA
                     GDVIPQVISALPQKRPKGARKPVPPKECPACGTEVIKPEDSVFSICPNRSGCPGQIFQ
                     HIKHFVSRGAMDIEGLGEKQVARFLEEGLISDVADIYALRQEQLEALDRMGETSSGNL
                     IAEIEKSREIPFPRVLYALGLIGVGSVTAEALADEFGSIDALHEADPERIEQTEGVGP
                     VIARQIAESLADEGTWALVERLKAAGLKMELAEDERRPEGGPLEGKTVVLTGTLPDLT
                     RDEAGAMVKRAGGKVTGSVSKKTDFVVAGESAGSKLEKAEKLGVTVLDREGLDQLIAG
                     NLPPEPEGD"
     gene            complement(3153..>3586)
                     /locus_tag="PLJ64_11310"
     CDS             complement(3153..>3586)
                     /locus_tag="PLJ64_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94113.1"
                     /translation="SRSGKAGPTGPAGPTGSAGVTGTAGVTGPTGAVDASGFFTKAES
                     DARYPQVDVVQSSGEYVISVPSGTMQAIATLLCPDNSLAAKVGYEATGFDQGLVSAQL
                     DASTVDIVINNTGPNPANGTLDVKLNCFQIDPAIVPDPTFP"
BASE COUNT          640 a         1181 c         1214 g          551 t
ORIGIN      
        1 agttctgggt caccggcgac tccttcatgc agaacatggt ccggatcctg gtcggcacca
       61 tgctggaggt ggccgacggg agccgcagcc tggacaactt cgttcagctg ctggagggcg
      121 ccgaacgacc ggatgccggg gtcaccgccc cgccgcaggg cctctacttc gtgagagtcg
      181 attacccgga cccgccgccg ctgcccgttg cccggtgacg cggggcgcgc cgtcggctgg
      241 ccgccccccg ggtttcatat cctcaccctg tgaggattct tctgactaat gacgacggga
      301 tcggtgccaa gggcctgcag acgctgcggc gcgaactgcg gcagatcgaa ggcgtcaccc
      361 tggacgtgat cgctcccgac tcgaaccgca gcgcctcggc ccgctcgatc actacgcgca
      421 ccccgctttc ggtcgaggaa atccacttcg acgacggcga cctcggccac gcgactgacg
      481 gcaccccggt cgactgcgtg cgcttcgccc aggtcgggtt gctcggcgaa aagcccgacc
      541 tgatcgtctc cggcatcaat cacgggctca acctcggtga tgacgtcacc tattcgggca
      601 ccgtcgctgc cgccctggag gggatcctgc tcggtctgcc ggcggtcgct gtttcgcagc
      661 agtcggagaa gggggagatg ggctacatgc cgcagcgcca ttacgagttc gacacggtcg
      721 cgaagttcac tgccgggctg gtccgcgaga tgctcgacca tccgctgccc gaagacaccc
      781 tgctcaacat caacgcgccc gggggcgagg tgcagggcgt cgacgtgacc aaactgggca
      841 agcggctcta ccgcgacgag ctgaagaagg tggagcggga cgagaccaac ggccgtacct
      901 cctaccggat ctacggctac gagccgggcc tcgacgacga gcccggcacc gacatcggcg
      961 cgattgcccg cggccggctc acggtcaccc cgctcagcct ggactggacc catcacgacg
     1021 agatcgaagg cctgcgcggc cgcgatttcg aacggctgct gggagcggcc acctcttgag
     1081 cgaggaagag gcagcggccg gggaaccgtc acccgacgac gccagtcgga tcgccgaact
     1141 gcgtgaagtc ctgaaccatc acatcgtcct ctattacgag aaggacgacc cggagatctc
     1201 cgacgaggaa tacgaccggc ttttcgccga actccaggaa ctcgagggcc ggaacccgga
     1261 actgatcacc ggtgactcgc cgacgcagcg ggtcggcgcc ccggcttcga ccagcttcgc
     1321 cccggtcagg catgcggtcc cgatgctttc tctggccaac gccagaaccg aagaagagct
     1381 gaccgcctgg gagaacagga tccggaacca cctgctgcgc cgtgacgtgg acccgggcga
     1441 gatcaccttc gtcaccgaac cgaagatcga cggcctggcg atctcgctca cctacgagaa
     1501 cgggagactg accaagggcg cgacccgcgg tgacggtcag gtcggggagg acgtcaccct
     1561 caacatcgag acgatcagga cggtgccgaa gcagattgac gacgccccgc ccctgatcga
     1621 ggttcggggc gaggtctacc tgccgatcgc cgatttcgcc gagctcaacc agaagcgggt
     1681 tgacgccggc ctgcccgcct tcgccaaccc gcgcaactcc gcggccggat cgatccggca
     1741 gaacgacccg aaggccgcgg ccgagcgtcc gctcgcggtc tggtcatacg ggatcggggc
     1801 gatggacggg atcaggttcg ccagccactc cgaagagatc gagtggttgc gggaccacgg
     1861 cttccacgtc aaccccgaca tcgccgtcca cgactcgatc ggggaggtgc tggaccgctg
     1921 ccactggtgg ctggatcgcc gcgaggacct cgacttcgag atcgacggcg tggtcgtcaa
     1981 gatcgatgac atcggcctct ggaacgagct cggcaacgcc ggccgcgagc cgcgctgggc
     2041 gatcgcctgg aagttcccgc cgaccacggc gaccacgaaa ctgctggaaa tcgagtggaa
     2101 cgtggggcgg accggccacc tggtgccctg ggcgaagctc gacccggtcc gggtcggcgg
     2161 ggtcaccgtc tccaacgcca cgctccacaa cgaggaggat ctcgaacgca aggacgtgcg
     2221 ggccggcgac gaggtggtgg ttctgcgggc cggggacgtg atcccccagg tgatttccgc
     2281 cctgccacag aagcggccga agggggcgag gaagccggtc ccgccgaagg agtgcccggc
     2341 ctgcgggacg gaagtgatca agccggagga ttcggtcttc tcgatctgcc cgaaccgttc
     2401 cggctgcccc ggccagatct tccagcacat caagcacttc gtctcccgcg gagcgatgga
     2461 catcgagggc ctcggtgaaa agcaggtcgc ccgtttcctt gaggaaggcc tgatcagcga
     2521 cgtcgctgac atctacgccc tgaggcagga gcagctcgag gcgctggacc ggatggggga
     2581 gacctcctcc ggcaacctga tcgccgagat cgagaagtcc cgggagatcc ccttcccccg
     2641 ggtcctctac gcgctcggtc tgatcggcgt tggatcggtt acggccgaag ccctggccga
     2701 cgagttcggt tcgatcgacg cgttgcatga ggccgacccg gagcggatcg agcagaccga
     2761 gggggtcggt ccggtcatcg ctaggcagat cgccgagtca ctggccgacg aagggacctg
     2821 ggctctggtc gagcgcctca aggcggccgg cctgaagatg gaactggccg aggatgagcg
     2881 tcgtcccgag ggcggcccgc tggaaggcaa gaccgtcgtc ctgaccggca ccctgcccga
     2941 cctgacccgt gatgaagccg gggcgatggt caagcgggcc ggtggcaagg tcaccggttc
     3001 ggtctcgaag aagaccgact tcgtggtggc cggagagtca gccgggtcca aactcgaaaa
     3061 ggccgagaag ctcggggtga ccgttctcga ccgcgagggc cttgaccagc taatcgccgg
     3121 gaacctccct ccggagcccg aaggggactg attcagggaa aggtcggatc cggcactatc
     3181 gccgggtcga tctggaagca gttgagcttg acgtcgagag ttccgtttgc cgggttgggt
     3241 ccggtgttgt ttatgacgat gtcgacggtg ctggcgtcga gctgggcgga aaccaggccc
     3301 tgatcgaagc cggtggcctc atagcccacc ttcgccgcga gggaattgtc cgggcagagc
     3361 agtgtcgcga tcgcctgcat cgttccggaa ggaaccgaaa tcacgtactc cccgctcgac
     3421 tggaccacgt cgacctgtgg gtagcgggcg tcgctttccg ctttcgtgaa gaaaccggag
     3481 gcgtcgaccg ctccggtggg tccggttacg cccgccgttc cggtcacgcc ggccgatccc
     3541 gtcgggccgg ccggaccggt cgggccggcc ttgccggacc tgctga
//
LOCUS       DAOQKN010000216         3585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_16102_length_3585_cov_4.140227, whole genome shotgun sequence.
ACCESSION   DAOQKN010000216 DAOQKN010000000
VERSION     DAOQKN010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16102_length_3585_cov_4.140227"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..507
                     /locus_tag="PLJ64_11315"
     CDS             145..507
                     /locus_tag="PLJ64_11315"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNC94114.1"
                     /translation="MSEEKSDSELLGAFAKEARQRCDVISAGLDTGTSDFETLRQEAH
                     ALHGTASTLGLRRLGELAGLMESDLSDAAKAGEIRPNRASQISEAAEALATGVKAAAD
                     GEEEPPDVGRSLSQLFAL"
     gene            940..1013
                     /locus_tag="PLJ64_11320"
     tRNA            940..1013
                     /locus_tag="PLJ64_11320"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:974..976,aa:Ile,seq:cat)
     regulatory      1149..1291
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            1294..1899
                     /locus_tag="PLJ64_11325"
     CDS             1294..1899
                     /locus_tag="PLJ64_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94115.1"
                     /translation="MSIRKLLLPGVVAVLAFLGGQGTAGATTPVYLNDGTITAKDVRS
                     LIFNATDRTQTRGRITPDVALIDAGQLKIRSASCKKGGDWAGYGVLFSGKREGGTRCD
                     GKTFSRRTGAYGGPFYGMWRMRFILDYVTSEGGLEGLGSGRTSWIKSNGEVAVLVFRI
                     YANGKATNTDKVLRIVGSEVTVTELRPGERWPFPLLYHRRA"
     gene            complement(1915..>3585)
                     /locus_tag="PLJ64_11330"
     CDS             complement(1915..>3585)
                     /locus_tag="PLJ64_11330"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HNC94116.1"
                     /translation="FIIQGVDYFAAADGIHSPVQHYWSLSVEEQFYVVWPLILLAAGA
                     LAAKFRRPTKGLVLIFIVPVAIASLIYSIHFTQVNPEAAFFSTIARAWQLAFGAVLAM
                     ILPKKIGMPAWLATVIGVIAVGVLGWATYTMSASTPFPGSNGLYPVGATIALLVVGAS
                     GAGITTRILSTRPFQYLGNISYSWYLWHWPFVVFALGIFDNLNWHWLVVATLISWIPT
                     QISHTLLEDPVRRAKILKLRPNRALAIGGACSIAAAAFGLWLGSNRINVNILDQDQVA
                     GAAVIKNGHIPLVNSVDSIKPNPLHARDDRGQMYADGCLLIGPRTESGECVYGDPDGD
                     VSVVLFGDSHSMMYMPALDAIGKEKGWKITGLSRANCVIADVKLSQYCDEWRENSLER
                     IQEEHPDLVVVATSTQAVYSLKKNGKQLSRLASEPYLVDGMARTFRQLEEDADQVVLL
                     RDQAQAPRQPWLPHECVAENLHNLKTCAFAPKRHPELGFDVDAAEKTGVQIIDPMPIL
                     CSDGRCPVVIGNALVYRDSYHVSATYMVTMAPWLEQELAKLNFPANSR"
BASE COUNT          686 a         1076 c         1200 g          623 t
ORIGIN      
        1 tcgctccatt ccgtaggatg aaaaccagcg ggagcctatg ccagattcgg gtggtttcga
       61 ccggcgagta tgcaccgacc gcgtcaaacg gcaagaatgg tggcgcctga ccgaagtttt
      121 cgtccgagat ctacgaggtg agctatgagt gaggagaagt cagacagcga gctgctcggt
      181 gctttcgcca aggaagcacg tcagcgttgc gacgtcatct cggccggtct cgacaccggc
      241 acgagcgact tcgagactct ccgccaggaa gcgcacgcac ttcacggcac cgcatccact
      301 ctcggcctgc gccgcctcgg ggaactcgcc gggctcatgg agtccgacct ttccgacgcc
      361 gccaaggcgg gcgagatacg cccaaaccgg gccagccaga tatccgaggc cgcggaagct
      421 ctcgctacag gcgtgaaggc cgcggccgac ggggaagagg aaccacccga cgtgggccgc
      481 tccctctccc agctcttcgc cctctaagcc gggacagccc cggcccgggc aacgcgccca
      541 ggacgcaggc atctgcatgc cgtccggtct tcgccggacg gccaatgaaa ctcgcccctt
      601 cctctcacat tcactgacgg taggaagggg ctccgctagg tgtttgacga cgtgagtact
      661 gtcgcggcct ttcccggctt actgaccgcc agccacccgc aggagcgccc tgtgcacgac
      721 ccgccggagg tttgtggtga ggatgtttca cgagatccgg tttatcagcg aatagcgcac
      781 ccaactccga tctcgaccgg ttcccgcccc gaacccgggg caggtgccta tggatttcgt
      841 tcatatagac gaaatccaca ggcacctcgg tgccgtcgga cccgccacgc gcgggtaggg
      901 gtcaggcaaa tatctttggg ggcagggtgc aagcccggcg ggccggtagc tcagttggtt
      961 agagcagcgg actcataatc cgtaggtcga aggttcgagt ccttcccggc ccactcgctt
     1021 gcagacgcga acatctttcc cacggcgacc tggacgattc cgcttggggg ataggcttgg
     1081 gcatgctgtc ccggggtcca atgcgattgt tcgagcgccg ccggttgggt tagggattgt
     1141 gtagcatcgg atgtgcaaag cctaactcgg atcgattccg gggcggggga accaattggg
     1201 acgagatccc cggggcgagt cgccatgcgt agcgcggtct cttttcgcgc gagcccgaca
     1261 gctaaccccg caggcaagaa aaagagaggg aacatgtcga taaggaagct gctgttgccg
     1321 ggcgtcgtcg ccgtattggc cttccttggg ggccagggaa cggccggggc caccacgccg
     1381 gtctatctga acgacgggac catcaccgca aaagacgttc gcagtctgat cttcaacgcg
     1441 accgatcgga cccaaacccg gggccggata accccggacg tggcgttgat cgacgccgga
     1501 caattgaaga tcaggtcggc ctcctgtaag aagggcggcg actgggcggg ctacggcgtg
     1561 ctgttctccg gaaaacggga aggcggaacc cgttgcgacg gaaaaacgtt ctcgcgccgg
     1621 accggggcat acggtggccc gttctacggc atgtggagga tgcggttcat cctcgactac
     1681 gtaacttccg aaggcggcct cgagggcctg ggctccggcc ggaccagctg gattaagtcg
     1741 aacggggaag tggcagtgtt ggtcttcagg atttacgcga acggcaaggc gaccaacacc
     1801 gacaaggtgc tccgtatcgt cggctcggaa gtcacggtca ccgaactgcg cccgggtgag
     1861 agatggccgt tcccgcttct gtatcaccgc agggcataac gacctaggtc cgggctaacg
     1921 ggagttagcg gggaaattta gcttcgccag ttcctgttcc agccaggggg ccatcgtcac
     1981 catgtaggtg gcggacacgt ggtacgaatc gcggtagacg agggcgttgc cgatcactac
     2041 cgggcagcgg ccgtccgagc agaggatcgg catcgggtcg atgatctgga cgccggtttt
     2101 ctcggcggcg tcgacatcaa agccgagctc cggatgccgt ttcggggcga aggcgcaggt
     2161 cttgaggttg tggaggtttt cggccacgca ttcatggggc agccagggct ggcgcggagc
     2221 ctgggcctgg tcgcggagca ggacaacctg gtcggcgtcc tcctcaagct gccggaaggt
     2281 cctcgccatg ccgtcgacca ggtaaggctc gctggcgagt cggctcagct gcttgccgtt
     2341 cttcttcagg ctgtagacgg cctgggtcga ggtggcgacc acgaccaggt cggggtgctc
     2401 ttcctggatt cgttccagcg agttctcccg ccactcgtcg cagtactggc tcagtttcac
     2461 gtccgcgatg acgcaattgg cccgggagag gccggtgatc ttccagcctt tctccttgcc
     2521 gatcgcgtcc agcgccggca tgtacatcat cgaatgggaa tcaccgaaaa ggactaccga
     2581 aacatcgccg tcgggatcgc catatacgca ttcgccggat tcggtgcggg ggccgatcag
     2641 caggcagcca tcggcataca tctggccgcg gtcatcgcgg gcatggagcg gattgggttt
     2701 gatcgagtcg accgaattga ccagcgggat gtgtccgttt ttgatcaccg cggcaccggc
     2761 cacctgatcc tggtcgagga tgttcacgtt gatccggttc gatccgagcc acaggccgaa
     2821 agcggcggca gcgattgaac aggcaccacc gatcgccagg gcccggttcg gcctcagctt
     2881 gaggatcttc gccctccgta cgggatcctc gagcagggtg tggctgatct gggtcgggat
     2941 ccaggagatc agggtggcga cgaccagcca gtgccagttc aggttgtcga agatgccgag
     3001 cgcgaagacg acgaagggcc agtgccacag ataccaggaa tacgagatgt tgccgaggta
     3061 ctggaagggc cgggtcgaga ggatccgggt ggtgatgccg gcaccggagg caccgaccac
     3121 aagcagggcg atcgtcgccc cgaccgggta gaggccgttg gaaccgggga atggggtgga
     3181 agccgacatc gtgtaggtcg cccagccgag caccccgacc gcgatcaccc cgatcacggt
     3241 ggccagccag gccggcatgc cgatcttctt cggcaggatc atcgccagca ccgcgccgaa
     3301 ggcgagctgc caggcccggg cgatggtcga gaagaaggcc gcctcggggt tcacctgggt
     3361 gaagtggatg ctgtagatca gcgaggcgat cgcgaccggg acgatgaaga tcaggaccag
     3421 gcccttggtc ggacggcgga acttcgcggc cagggcaccg gcggccagca ggatcagcgg
     3481 ccagaccaca tagaactgct cttcgaccga cagcgaccag tagtgctgga ccgggctgtg
     3541 aatgccgtcc gcggcggcga aataatcgac gccctggatt atgaa
//
LOCUS       DAOQKN010000217         3577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_16164_length_3577_cov_5.242760, whole genome shotgun sequence.
ACCESSION   DAOQKN010000217 DAOQKN010000000
VERSION     DAOQKN010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16164_length_3577_cov_5.242760"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="PLJ64_11335"
     CDS             complement(<1..390)
                     /locus_tag="PLJ64_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNC94117.1"
                     /translation="MTEPRRDSRWWGWGDPDVPTELGEAAERMLGERGISTGADASPP
                     EIDRVEIPEAVPVDERVLSAAGAENVFTGNEDRIRHANGQNYLDLLELREGRLQHAPD
                     AVIVADSGPRIAAILEACASAGVAVVPF"
     gene            complement(387..845)
                     /locus_tag="PLJ64_11340"
     CDS             complement(387..845)
                     /locus_tag="PLJ64_11340"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNC94118.1"
                     /translation="MTRKKAVAATPEKVYDLISDPSRLADWWPRVVRVEDIAGGPGAA
                     RTRWTSVLGADSGRMLRLDFRCSGATRPERYEWEHELEGTPYESHLARQAIEARITGD
                     GEGSQVSLTSTNVLRGTAKIAGFSMKKGQRELLDEALESLAETLEPGGGS"
     gene            complement(863..1603)
                     /locus_tag="PLJ64_11345"
     CDS             complement(863..1603)
                     /locus_tag="PLJ64_11345"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNC94119.1"
                     /translation="MTLSEQNLAEGTPMAPRRPRKALTIGAVAKLLSKEFDDVSISKI
                     RYLEDQKLVTPRRTPGGYRLYSQADVDRLRTVLRLQRDEFLPLRVIRQELAAGPSVTG
                     LSGNSSNTARRAKLIGTTSSRFTVEEVIEETGARAEFIRELAEFGLVTPRSENGRTVY
                     DETDMEIVRAANELARSGVGARNLKLFKTSADREVNLIETLLAPKLASRNPQRRKEAV
                     ESLERMATVLSELKHALLVRDLRRLADS"
     gene            complement(1605..2075)
                     /locus_tag="PLJ64_11350"
     CDS             complement(1605..2075)
                     /locus_tag="PLJ64_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNC94120.1"
                     /translation="MATHCPECGFVNPEAANYCQKCGTFIGRREGGDVQGTSTYQVGE
                     TGEIEAVDIDAEVERTGAALVIRSGGGRAGETFPMEEERVAIGRSPDAGVFLDDVTVS
                     RNHALLVSRGDGTYIDDLGSLNGTYVNRKRIESQRLEDGDEIQVGKYKLTYLER"
     gene            complement(2118..2780)
                     /locus_tag="PLJ64_11355"
     CDS             complement(2118..2780)
                     /locus_tag="PLJ64_11355"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNC94121.1"
                     /translation="MGDSAPFGKGRVRKLFGLDRTGAEPAPTRAGQPLRPFTIPNFIG
                     YLRLLGIPLFLYVAFSSDDGRDWTAALLFFLIAGGDYVDGFLARVTGQYSRLGALMDP
                     VIDRLTIFSGLVVCWHFELLPRWLLALLLVRELATLLLARWGLSHGVDIEVNWFGRVA
                     VFPVMGALFLALIWDSWVPAVMLGVGVVLGVIATWLYARVGRDRIHALEAAARPEETA
                     QP"
     gene            complement(2780..>3577)
                     /locus_tag="PLJ64_11360"
     CDS             complement(2780..>3577)
                     /locus_tag="PLJ64_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HNC94122.1"
                     /translation="EIKLANALGVQSRPAGEEEIAEKLGPAGFIGPVGASVRILKDRA
                     VTGGGLVAGANRPDLHLKGVEPGRDFAFEEVDIRTVVEGDTTDDGHSITLEPAIEIGN
                     IFKLGTRYTEPLGATFLDENGEEKPVIMGCYGIGPARIVAAAAEQYADENGLSWPVSL
                     APWDIQLVSLSKSDEPERVEADRLYEELLEQGFDVLYDDRDAGPGQKLTDAELLGCPL
                     RVVVGRRGLADGIYEVSERRSGEVHRVPVEGGAAGIAGLYAAISGSD"
BASE COUNT          572 a         1215 c         1165 g          625 t
ORIGIN      
        1 gaagggcacc accgcgactc cggccgaggc acaagcctcg agaatcgctg cgatccgcgg
       61 gccggagtcg gcgacgatca ccgcgtcggg agcgtgctga agccggccct cgcgcaactc
      121 gagcaggtcg agatagttct ggccgttggc gtggcggatc cggtcctcgt tcccggtgaa
      181 cacgttttcg gcaccggcag cgctcaggac acgttcatcg accggcacgg cttccggaat
      241 ctcaacccgg tcgatctccg gtggcgaggc gtcggcgccg gtgctgatgc cacgttcgcc
      301 aagcatccgc tccgcggctt cgccaagttc ggtcggcaca tccgggtcgc cccagcccca
      361 ccagcggctg tcgcgccgtg gctcggtcaa gacccgccgc ccggttcgag ggtctcggcg
      421 agggattcga gcgcttcatc gagcagctcc cgctggccct tcttcatcga gaagccggcg
      481 atcttcgcgg ttccccgcag aacgttggtc gaggtgaggc tcacctgcga tccctcaccg
      541 tcgccggtga tccgggcttc gatcgcctgg cgggcaaggt gggactcgta cggggttcct
      601 tcgagttcgt gttcccattc gtagcgctcg ggccgggtcg cgccggagca gcggaagtcg
      661 agccggagca tccggccgga atcggcaccg agcacgcttg tccagcgggt tctcgcggcc
      721 ccgggtccgc cggcgatgtc ttccacccga accaccctcg gccaccagtc ggccaggcgg
      781 gacggatcgc tgatcaggtc atagaccttc tccggcgtcg ccgccacagc cttctttcgg
      841 gtcaccgtcg gcacggcctg gatcagctgt cggcgagtcg gcgcaggtca cgcaccagca
      901 gggcgtgttt cagctcgctc agcacggttg ccatccgctc gaggctttcg accgcctcct
      961 tgcgacgctg cgggttgcgg gaagcaagct ttggcgccag cagggtttcg atcaggttga
     1021 cctcgcggtc ggccgaggtc ttgaagagct tcaggttgcg ggcgccgacc ccggagcggg
     1081 ccagctcatt cgccgcacgg acgatctcca tgtcggtttc gtcgtacacg gtcctgccgt
     1141 tctcgctgcg cggagtgacc agaccgaatt ctgcaagttc ccgaatgaat tcggcccggg
     1201 cgccggtctc ctcgattacc tcttccaccg tgaaccggga gctggtcgtg ccgatcagct
     1261 tggcccgtcg ggcggtgttc gatgagttgc cggacagccc ggtaaccgaa gggccggccg
     1321 caagttcctg gcggatcacc cgcaacggca ggaactcgtc gcgctggagc cgcagcacgg
     1381 tgcggagccg gtcgacgtcg gcctggctgt aaagccggta tccgcccggc gtgcgcctgg
     1441 gagtgaccag cttctggtcc tcgaggtagc ggatcttcga gatcgaaacg tcatcgaatt
     1501 ctttgctgag cagcttcgcc accgccccga tggtcagggc cttgcggggc cgcctcggag
     1561 ccatcggcgt gccttcggcg aggttctgtt cgctcagcgt catctcacct ctccagatag
     1621 gtcagcttgt acttaccgac ctgaatctcg tcgccgtcct cgagccgctg ggactcgatg
     1681 cgcttgcggt tcacataggt tccgttgagc gatccgagat cgtcgatgta ggtgccgtct
     1741 ccgcgggaaa ccagcagcgc gtgattgcgg gaaaccgtga cgtcatcgag gaagacgccg
     1801 gcatcgggac tgcgaccgat cgccacccgt tcctcttcca tcgggaaggt ctctccggcc
     1861 cgtccgccgc cgctgcggat gaccagggcg gcaccagtgc gttcaacctc ggcgtcgatg
     1921 tcgactgctt cgatctcgcc ggtttccccg acctggtagg tcgaggttcc ctgtacgtct
     1981 cccccttcgc ggcggccaat gaaggttccg cacttctggc agtagttggc ggcctcgggg
     2041 ttgacaaatc cgcattcggg gcaatgggtc gccatagcgg ccattatagg ggccgaccct
     2101 aaccttcaac tcaggcttta cggttgggcg gtttcttccg gcctggccgc tgcttccagg
     2161 gcgtgaatcc ggtcccggcc caccctggcg taaagccagg tcgcgatcac tccgagaacc
     2221 acgccgacgc cgagcatcac ggccgggacc cagctgtccc agatcagggc caggaacagg
     2281 gcgcccatca cggggaagac ggcgacccgg ccgaaccagt tcacctcgat gtctaccccg
     2341 tgcgagagcc cccagcgggc cagcagcagg gtggccagtt cccggaccag aagcagcgcc
     2401 agaagccagc gcggcagcaa ctcgaaatgc cagcagacga ccaggccgga gaagatcgtg
     2461 agccggtcga tcaccgggtc catcagggcc ccgaggcggc tgtactgacc ggtcacccgg
     2521 gccaggaagc cgtcgacgta atcgcccccg gcgatcagga agaagagcag tgcggcggtc
     2581 cagtcgcgac cgtcatccga gctgaaggcg acatagagaa agagcgggat gccgagcagc
     2641 cggaggtagc cgatgaagtt ggggatcgtg aacggccgaa gcggctgccc ggcccgggtc
     2701 ggggccggtt cggccccggt ccggtcgaga ccgaaaagtt tgcggaccct gcccttgccg
     2761 aagggagcgg agtcccccat cagtccgatc cggagatcgc tgcgtacagc ccggcaatac
     2821 cggcggcacc accctcgacc gggacacgat gcacttcacc gctccgcctt tcgctgactt
     2881 cgtagatgcc gtcggcgagc cctcgccggc cgaccacgac cctgagcgga cagcccagca
     2941 gctcggcatc ggtcagcttc tggccgggcc ctgcgtcccg gtcgtcgtag agcacgtcga
     3001 agccctgctc gagcagttcc tcatagagcc ggtccgcctc gacccgctcc ggctcgtcgg
     3061 atttcgaaag cgacaccagc tgaatgtccc agggtgccag gctgaccggc caggaaaggc
     3121 cgttctcgtc ggcatactgc tcggccgcgg cagccacgat ccgcgccggt ccgatcccgt
     3181 aacagcccat gatcaccggt ttctcctccc cgttctcatc gaggaaggtg gctccgagcg
     3241 gctctgtgta gcgagtgccc agcttgaaga tgttgccgat ctcgatcgcc ggctcgaggg
     3301 tgatcgagtg gccgtcatcg gtggtgtcgc cttcgaccac ggtgcggatg tcaacctctt
     3361 cgaaggcgaa gtcccggccc ggctcgaccc ccttcagatg aaggtcgggg cgattggctc
     3421 cggcgaccag gccgccaccg gtgaccgcgc gatccttgag gatccgcacc gaggcaccga
     3481 ccgggccgat gaacccggcc gggccgagtt tctccgcgat ctcttcttcg ccggccgggc
     3541 gcgactgaac gccaagtgcg ttggcgagtt tgatctc
//
LOCUS       DAOQKN010000218         3539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_16495_length_3539_cov_7.078932, whole genome shotgun sequence.
ACCESSION   DAOQKN010000218 DAOQKN010000000
VERSION     DAOQKN010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16495_length_3539_cov_7.078932"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1402)
                     /locus_tag="PLJ64_11365"
     CDS             complement(<1..1402)
                     /locus_tag="PLJ64_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94123.1"
                     /translation="MSRIIRVFLLALLISTVVSVPARAGTIVADSGFRPGTDGFSFAN
                     YGADEGYRNLDAFEVQKIYGRAACLTGKGATCVLNPGVRNWMRSTNEAMAGGHCYGFS
                     ALTQLIYKNELPRFGYSSISAFGGSSPFGLNIVGNVRLQRSIARAFTYQLLPSVNAQA
                     TMGTPKHVLRYLIDHLGDGSQQSWNLLIFQWGFQAGHAITPYAIEDMGGGIYEIHVYD
                     NNWPNDDTRRLVVNTNRNTWSYYASTQPGIPAAEYRGNARSGTLFLRPNTPALGIQPC
                     PYCIGRQGSNSKYNQVTLSYTADQHARLLITDSKGRQTGFKDGKPINRIPGAKVIRQA
                     TSPITFAADGAIENIADDPEPVYLIPKNLKLRIRIDGRHMTVTDRESLGVVGPTFDST
                     VENLKMGPSKVAFATLSPKAKTLSITGARGESSPRVTFGAQSRKAAYRVKVSAIGAAP
                     QSTFYFAKKPNYGLLRI"
     gene            1540..1613
                     /locus_tag="PLJ64_11370"
     tRNA            1540..1613
                     /locus_tag="PLJ64_11370"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1574..1576,aa:Lys,seq:ttt)
     gene            complement(1644..2420)
                     /locus_tag="PLJ64_11375"
     CDS             complement(1644..2420)
                     /locus_tag="PLJ64_11375"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HNC94124.1"
                     /translation="MNVDVTTRGSVDPEAVELAQEKLTELESAVGRPLSHVRVVLRQE
                     KESIPNAARAEGEAQVGKTIIRGHVAADSMSHAVHELADRLQQQIRRHLDRVTSLKRQ
                     PVETPDGKWDHGTWVPARPAQSLLAPGEREVIRRKVLSLVPLKASEAAELMRELDHGF
                     YLFHDSDTEADSVIYLRDDGQLAVIAAQGVAAPADGEDSSGIQRETSHYSSALTLEDA
                     VSQMDELNHRFMFFTDAATDRAAIIYLRYDGNYGLIEQEA"
     gene            complement(2477..>3539)
                     /gene="thiS"
                     /locus_tag="PLJ64_11380"
     CDS             complement(2477..>3539)
                     /gene="thiS"
                     /locus_tag="PLJ64_11380"
                     /inference="COORDINATES: protein motif:HMM:NF017501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HNC94125.1"
                     /translation="PSATSVGVPFNSIRIKTGGGLMRIELNGTPTDVAEGLTITEVIG
                     LVAGERIPEDRRGVAVALRGEVVPRSEWDSTVPAEDDPVEVVAAIQGGSGEVDSGQWE
                     LGGRTWTSRLIAGSGGFRSLEAMESALALSGTEIITVALRRLDPSAQGSVMDVIEKLG
                     LFILPNTAGCYTARDAVRTAKLAREAFGTEWVKLEVIGDDRTLLPDAPELLRAAEELV
                     GDGFIVLPYTNDDPILAARLEATGCAAVMPLGGPIGSGAGIRNPYNISIIVENAKVPV
                     ILDAGIGTASDATLAMELGCDAVLLASAVSRAEKPDLMAVAMKHAVEAGRAARQAGRI
                     PKRLYAEASTPEEGLPGLG"
BASE COUNT          564 a         1124 c         1195 g          656 t
ORIGIN      
        1 cgatcctgag cagaccgtaa ttgggcttct tggcgaaata gaaggtcgac tgcggggccg
       61 cgccgatcgc actcaccttg acccggtagg cggccttcct gctctgggcc ccgaaggtga
      121 cccgcggcga ggattcaccc ctggccccgg tgatcgaaag ggtcttggcc tttggcgaaa
      181 gggtcgcgaa tgcgaccttg ctcggtccca tcttcaggtt ctcgacggtc gagtcgaagg
      241 tcgggccgac cacccccagc gattcgcggt cggtgaccgt catgtgcctg ccgtcgatcc
      301 ggatccggag cttcaggttc ttggggatga ggtagaccgg ttcgggatcg tcggcgatgt
      361 tttcgatcgc gccgtcggcc gcgaaggtga tcggcgaggt cgcctgccgg atgaccttgg
      421 ccccggggat ccggttgatc ggcttgccgt ccttgaaccc ggtctggcgc cccttcgaat
      481 cggtgatcag cagccgtgcg tgctgatcgg ccgtgtagga gagggtgacc tggttgtact
      541 tcgagttgga tccctggcgg ccgatgcagt acgggcaggg ctggatcccg agggccggcg
      601 tgttcgggcg gaggaaaagg gttcctgaac gggcgttgcc cctgtactcg gcggcgggga
      661 tgccgggctg ggtggacgcg tagtagctcc atgtgttccg gttcgtgttg acgaccagcc
      721 tgcgggtgtc gtcattcggc cagttgttgt catagacgtg aatctcgtag atgccgcccc
      781 ccatgtcctc gatcgcgtat ggggtgatcg cgtgaccggc ctggaagccc cactggaaga
      841 tcagcaggtt ccaggactgc tgagagccgt ccccgaggtg gtcgatcagg taccggagca
      901 cgtgcttcgg agtgcccatc gtcgcctgcg cgttgaccga gggaagcagc tggtaggtga
      961 aggcccgggc gattgaccgc tggagacgga cgttgccgac gatgttcagg ccgaaggggg
     1021 agctgccgcc gaaggcggag atcgagctgt aaccgaagcg cgggagctcg ttcttgtaga
     1081 tcagctgggt gagcgcggag aacccgtaac agtgcccgcc ggccatcgcc tcgttggtcg
     1141 acctcatcca gttgcggacg ccggggttca gaacgcaggt cgcgcccttc ccggtcaggc
     1201 aggccgcccg gccgtagatc ttctgcacct caaaggcgtc caggttcctg taaccctcgt
     1261 cggcgccgta gttggcgaac gaaaacccgt ccgttccggg ccggaagccg gagtccgcaa
     1321 cgatcgtccc cgcccgggca ggcacggaaa ccacggtcga gatgagcagg gccagcagga
     1381 aaacgcggat gattctggac acactcgcaa ggtacccaat tgaggcgagt cagtatcgcc
     1441 accggacaga gccggcccat ccatcagttg cttctgtggg ccggcgggtt tgacttggag
     1501 gcggagcgaa gctcgccgac ttgcagttaa gctccgcggg cccccttagc tcagttggtt
     1561 agagctgcag gcttttaacc tgtgagtcct gggttcgaat cccagagggg gcatttgctg
     1621 cggcgcggcg agcgccgcga atctcaggcc tcttgttcga tcaggccgta gttgccgtcg
     1681 tagcgcaggt agatgatcgc ggcgcggtct gtggcggcat cggtgaagaa catgaagcgg
     1741 tggttcagtt cgtccatctg gctcaccgca tcctcaaggg tcagggccga ggagtaatgg
     1801 ctggtttcac gctggattcc ggatgagtct tccccgtccg cgggggcggc gacgccctgt
     1861 gcggcgatca ccgcgagttg gccatcgtcc cggaggtaga tgaccgaatc ggcctcggta
     1921 tccgagtcgt ggaagaggta gaagccatgg tccagctccc tcatcagttc cgccgcctcg
     1981 gacgccttca gcggcacgag tgaaaggacc ttgcgccgga tgacttcccg ctctccggga
     2041 gcaagcaatg actgagccgg ccgggccggt acccatgttc cgtgatccca cttgccgtcc
     2101 ggggtctcga ccggctggcg cttgaggctg gtgacccgat cgaggtgccg ccggatctgc
     2161 tgctgaagcc ggtcggccag ctcgtgaacc gcgtgggaca tgctgtccgc ggccacgtgg
     2221 ccccgaatga tcgtcttgcc cacctgcgct tcgccctcgg cccgggcggc gttcgggatg
     2281 ctctccttct cctgccgcag caccacccgg acgtgactca gcggacggcc gacggcggac
     2341 tcgagttcag tgagtttctc ctgggccagc tcgactgctt cgggatcaac cgaaccacgg
     2401 gtcgtaacgt cgacgttcat cgccagatca taggtgacgc gttcgaatcc ccgaggggca
     2461 ccttcatgcg tcgggcttag ccgaggccgg ggaggccttc ttcgggggtg ctggcttcgg
     2521 cgtaaaggcg cttcgggatc cggccggcct ggcgggcggc gcggcccgct tcaaccgcgt
     2581 gtttcatggc aaccgccatc aggtccggct tttcggcccg ggaaaccgcg ctggccagga
     2641 gcaccgcgtc gcagccgagt tccatcgcca gggtggcgtc ggaggcggtg ccgatcccgg
     2701 cgtcgaggat caccggcacc tttgcgttct cgacgatgat cgagatgttg tacggattgc
     2761 ggatccctgc gcccgagccg atcggcccgc ccagcggcat caccgcggcg cagccggtcg
     2821 cctccagccg ggcggcgagg atcgggtcgt cgttggtgta aggcagcacg atgaagccgt
     2881 caccgaccag ctcctcggcc gccctgagca gttcgggagc gtcgggaagc agggtgcgat
     2941 cgtcaccgat cacctcgagt ttgacccact cggtgccgaa ggcttcccgc gcgagcttcg
     3001 ccgtgcggac cgcgtcgcgg gcggtgtaac agccggccgt gttcggcagg atgaacagcc
     3061 cgagcttctc gatcacgtcc atgaccgaac cctgggccga aggatcaagc cgccgcaggg
     3121 cgacggtgat gatttccgtt cccgaaagtg ccagggcgct ttccatcgct tccagcgagc
     3181 ggaagccgcc actgccggcg atcagtctcg aggtccaggt ccggccaccg agttcccact
     3241 gtcccgaatc cacttccccg gagcccccct ggatcgcggc aacgacctca accgggtcgt
     3301 cttcagccgg aaccgtcgag tcccactccg agcgcgggac gacctccccc cggagcgcga
     3361 ccgcaactcc gcgccggtct tcggggatcc gttcccccgc aaccagcccg atcacttcgg
     3421 tgatcgtcag cccctcggca acgtcggtcg gggttccgtt caactcgatt cgcatcaacc
     3481 cacctcctgt tttgatgcga atcgagttga acggaacccc gaccgacgtt gccgagggg
//
LOCUS       DAOQKN010000219         3517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_16655_length_3517_cov_4.688908, whole genome shotgun sequence.
ACCESSION   DAOQKN010000219 DAOQKN010000000
VERSION     DAOQKN010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16655_length_3517_cov_4.688908"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="PLJ64_11385"
     CDS             complement(<1..628)
                     /locus_tag="PLJ64_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94126.1"
                     /translation="MSRFSFRLLVSVISIGVALGAGAGSASAMTVTSTGESGCSLRVA
                     IQAVAANDSGTACGAVVSGGPTPINLPANTITPFDGQIVVPAGVNVQINGDNVNNPLT
                     TVIDGTGATAGRIFEIKSGASVKLSGLTVTGGQALNGAAPTSRFASYSGQDGGGILNG
                     GNLTLDHVVLNGNHAGDGGDGWNGLLEESGGGAGWGGNGGGIYNSDGAS"
     gene            complement(720..1322)
                     /locus_tag="PLJ64_11390"
     CDS             complement(720..1322)
                     /locus_tag="PLJ64_11390"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC94127.1"
                     /translation="MEAAAEKQPRGPGRPSTGARERVLDAAVETLLEEGYAGLTYAKV
                     AVRAGENKSLISYYFGSKQGLVAAVADIIGERITVAVVSELNNVETVPDIAAGMIAGV
                     WKVMDEDPRLARLYFDLSAVSVVDDDVRDAIHLVKDRWREVIAGFMLDAGVRADRVAP
                     LGTFLMSGVQGLAIERLEGDQSDRLEAARKLFVRAAGAIA"
     gene            complement(1338..2318)
                     /locus_tag="PLJ64_11395"
     CDS             complement(1338..2318)
                     /locus_tag="PLJ64_11395"
                     /inference="COORDINATES: protein motif:HMM:NF012490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HNC94128.1"
                     /translation="MSENGSSGNGNGEVEFKTEDEALQVLLDGDIDKVMSGLDRIIEA
                     APSYEKLFMRWQRQHWSTEDFDFSVDAEQWNDPDFMTEEERRFMLFGFSQFFLGEERV
                     TVELLPFAIAAPSHEAKAFLTTQISDEAKHMVFFDRFYREVLGMKAADIGAMLETQRP
                     NVNKDWEKLFDGILHDCAERLRLDPTDFPALVRGVTVYMIVIEGTLALTGARFIIKSL
                     KERDWLPGFVSGFTAVNRDESRHVGFGVKFLADAIKADQANALIVQDTLRECLPITPL
                     VFAPSWVEDPYSFETPFYHSTEVFNYAMKALSKKLAAMGLDPAMLAGDPA"
     gene            2421..3239
                     /locus_tag="PLJ64_11400"
     CDS             2421..3239
                     /locus_tag="PLJ64_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94129.1"
                     /translation="MKRLAFLILLLPLAALAACGGETSPEDDVTEVANQILEAKSPEQ
                     VKVVCQELITPGMLRTVYGGELARCEDQPLNDDSGIEDPGEVSVASVTVEGGKADAAI
                     VTTGGSTDGNDGTWYFRQTDGDWKLDRIGDDFLRTSFAISIRNVDQGVVSYEPMRKCM
                     GREVEQLPASQIRGFMYKTMRGDRQAALDSVLKIAGACPRPMAEFVANELATRVLAKK
                     EVTPAQILCAKKKLVPLLQVTDLSSIALGSGDYGDATGLALAGLIAGVMKQCPA"
     gene            3290..>3517
                     /locus_tag="PLJ64_11405"
     CDS             3290..>3517
                     /locus_tag="PLJ64_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94130.1"
                     /translation="MPETADRQEITADKRDVAADGSIGMPSASDLERLRSKGRLRYFL
                     ALFGAAFVAAVAYVDPGNFATNVAAGAEYGYM"
BASE COUNT          639 a         1153 c         1115 g          610 t
ORIGIN      
        1 ggctcgcacc gtcggagttg tagatgccgc cgccgttgcc accccagccg gcgcctcccc
       61 cggactcttc gaggaggccg ttccagccat ctccgccgtc gccggcatgg ttcccgttga
      121 gcacgacgtg gtcaagggtc aggttgccgc cattcaggat tcctccgccg tcctggcccg
      181 agtaggaggc aaaacggctg gtcggtgcgg caccgttcag ggcctggccc ccggtgacgg
      241 tcaggcccga cagcttcacg ctcgcaccgc tcttgatctc gaatatccgg cccgccgtgg
      301 cgccggttcc gtcgatcaca gtggtcagcg ggttgttgac gttgtcgccg ttgatctgga
      361 cgttgactcc ggccggcacc acgatctgcc cgtcgaacgg cgtgatcgtg ttggccggaa
      421 ggttgatcgg ggtcggaccg ccgctgacca ccgccccgca ggcggtgccg ctgtcattcg
      481 cggccactgc ctgtatcgct acgcgcagcg agcagccgct ctcaccggtc gaggtgacgg
      541 tcatcgccga ggcagatccg gcgccggccc ccagagccac gccaatcgaa atgacggaaa
      601 ccagaagacg aaacgaaaac ctgctcacta tgaggtacct cttcccgata tggaccctgc
      661 gaaaacccca cccttgactc tcttaggttc accgggttat gtcaagggaa aagatccggt
      721 caggcgatcg cgccggcggc ccggacgaaa agcttgcggg ccgcttcgag gcgatcggac
      781 tgatcgccct cgagccgctc gatcgccagg ccctggacac cggacatcag gaaggtcccg
      841 agcggcgcga cccggtccgc ccgaacgccg gcgtccagca tgaagccggc gatcacctcg
      901 cgccagcggt ccttgaccag gtggatcgca tcacgaacgt catcgtcgac caccgagacc
      961 gcgctgagat cgaagtaaag ccgggccagg cgggggtcct cgtccatgac cttccagact
     1021 ccggcgatca tgccggcagc gatatcgggg acggtctcga cgttgttcaa ctcggagacg
     1081 accgcgacgg tgatccgttc accgatgatg tcggcgaccg cggcgaccag gccctgcttc
     1141 gacccgaagt aataggagat cagggacttg ttctcgccgg cccggaccgc gaccttggcg
     1201 taggtcagcc cggcgtagcc ctcttcaagc agagtctcga ccgccgcgtc gagcacgcgc
     1261 tcccgggccc cggtcgaagg ccgaccgggg ccgcggggct gcttttcggc tgctgcctcc
     1321 aatcgcgagc ctttggctca ggcagggtcg ccggccagca tggcgggatc gagacccatc
     1381 gccgccagct tcttcgacaa ggccttcatc gcatagttga agacctcggt cgagtggtag
     1441 aagggcgttt cgaagctgta cggatcctcg acccaggaag gggcgaagac cagcggcgtg
     1501 atcggcaggc attccctcag ggtgtcctgg acgatcagcg cgttggcctg atcggccttg
     1561 atcgcgtcag cgaggaattt gaccccgaag ccgacgtggc gggactcgtc ccggttcacg
     1621 gccgtgaagc cggagacgaa tccgggcagc cagtcccgtt ccttcagcga cttgatgatg
     1681 aagcgggctc cagtcagggc gagcgtgccc tcgatcacga tcatgtagac ggtgacgcca
     1741 cggactagcg ccgggaagtc ggtcgggtca agccggaggc gctcggcgca gtcgtgcagg
     1801 atcccgtcga agagcttctc ccagtccttg ttcacgttcg gccgctgggt ttccagcatg
     1861 gcgccgatgt cggccgcctt catgccgagc acctcgcggt agaagcggtc gaagaagacc
     1921 atgtgcttgg cctcgtccga gatctgggtg gtgaggaaag ccttcgcctc atgtgacggg
     1981 gcagcgatcg cgaagggcag aagctcgacc gtcacccgct cctcgccgag gaagaactgg
     2041 gagaagccga agagcatgaa gcgacgttcc tcctcggtca tgaagtccgg gtcattccac
     2101 tgctcggcgt caaccgagaa gtcgaagtct tcggtcgacc agtgctggcg ctgccagcgc
     2161 atgaacagct tctcgtagga aggagccgcc tcgatgatcc ggtcgagacc ggacatcacc
     2221 ttgtcgatgt caccgtcgag cagaacctga agcgcttcgt cttcggtctt gaactcgact
     2281 tcgccgttgc cgttacctga actgccgttt tcgctcatct ctctcctgaa gatctttgtg
     2341 gcctgggcga ataataaacg aacgaccaac aaataatcaa gtctgtttgc cgaagccggc
     2401 cggcgaggta cctaccgacc atgaaacgtc tcgccttcct catcctgctc cttcctctcg
     2461 ccgctctcgc cgcttgtggc ggcgaaacct cccccgagga cgatgtgacc gaagtcgcca
     2521 accagatcct cgaggccaaa tcccccgagc aggtcaaggt ggtctgccag gagctgatca
     2581 cccccgggat gctcaggacc gtctacggcg gcgagctcgc caggtgtgag gaccagccgc
     2641 tgaacgacga cagcggaatc gaggacccgg gcgaggtcag cgtcgcctcg gtcacggtcg
     2701 aaggcgggaa ggccgatgcg gcgatcgtca ccaccggcgg ttcaaccgac ggaaacgacg
     2761 gcacctggta cttcaggcag accgacggcg actggaagct cgaccggatc ggtgacgact
     2821 tcctgcggac cagcttcgcg atctcgatcc gcaacgttga ccagggcgtg gtcagttatg
     2881 agccgatgcg gaagtgcatg ggccgcgagg tcgagcagtt gccggccagc cagatccggg
     2941 gcttcatgta caagacgatg cggggtgacc ggcaggcggc gctggattcg gtcctgaaga
     3001 ttgccggggc ctgcccgcga ccgatggccg aattcgtcgc caatgaactg gcgaccaggg
     3061 tcctggccaa aaaggaagtc accccggccc agatcctctg cgcgaagaag aagctggtgc
     3121 cgctgcttca ggtcaccgac ctctcctcga tcgccctcgg gtcgggcgat tacggcgacg
     3181 cgaccggcct ggccctggcc ggactgatcg ccggcgtgat gaagcaatgc ccggcctaga
     3241 tatttagcca tcactcaaaa tcttttccga ggcactagta tccccggggt tgccagagac
     3301 tgccgacaga caggaaatca ccgccgacaa gcgtgacgtc gcggccgatg gctcgatcgg
     3361 catgccctcg gcctccgacc tcgagcggct ccgctcgaag ggccggctgc gctactttct
     3421 cgccctcttc ggggcggcct tcgtcgccgc cgtggcctat gtcgacccgg gcaacttcgc
     3481 gaccaacgtc gccgccggtg ccgagtacgg ctacatg
//
LOCUS       DAOQKN010000220         3504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_16772_length_3504_cov_6.170194, whole genome shotgun sequence.
ACCESSION   DAOQKN010000220 DAOQKN010000000
VERSION     DAOQKN010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16772_length_3504_cov_6.170194"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="PLJ64_11410"
     CDS             complement(<1..205)
                     /locus_tag="PLJ64_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94131.1"
                     /translation="MSESLLSGRWSIAIDPARRDGRIVESASEPAAEARLEPLPASLH
                     PGLAEALNRGGIEQLYSHQAEAFE"
     gene            complement(198..1145)
                     /gene="folP"
                     /locus_tag="PLJ64_11415"
     CDS             complement(198..1145)
                     /gene="folP"
                     /locus_tag="PLJ64_11415"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNC94132.1"
                     /translation="MASLPGTLRTAAGEINVAEPVIVGVVNATPDSFSDRGDRSTAGS
                     IRQVEQHLEDGAGIIEIGGESNVTNRPAVTPEEEIERILPVVVAAAAAGAIISVDTYK
                     PAVAAAALESGAAIINDISGGGNLELMGVAAEAGAGVVLMHTVVEPKTQRWDEAAYPE
                     GITAHQVRFFEERLESLAEIGIRRDQVVVDPGPDFGKTPAQTVDSLRGLPELCGLGCP
                     VMLAVSRKDFIGAINHRRPSERAPGTFAALAFGLDCGGRLLRVHDVAGTRDFLQVWRA
                     LRGESEVDPALRIADEIRREPPRDAAGSPNPAATSPEDV"
     gene            1153..2055
                     /locus_tag="PLJ64_11420"
     CDS             1153..2055
                     /locus_tag="PLJ64_11420"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF010496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HNC94133.1"
                     /translation="MLDTFHHGDFAAADISRKLRETVSVVIPARNTAGTIAATVAELQ
                     VLAEDGLVGQILVVDADSADGTAAVAREAGAEVVSENELVEGFGPCRGKGDAMWRSLA
                     AVTGEIVVFIDGDIADFGRHYVTGVLGPLTDPGKHFVKGRYQRPFRFGENEEPAGGGR
                     VTELAARPLLARLAPELLAFDQPLAGEVAARRELLDQVPFACGYGVEIAMLIEVWGRV
                     GIRGMAQVELGTKRNAHQSLAALSGMSSEVIAAMADTLDRLGRKDTGRMKMVEGSGPE
                     VVTRPAFATVPQVSHRSGRKPAAP"
     gene            2104..2913
                     /locus_tag="PLJ64_11425"
     CDS             2104..2913
                     /locus_tag="PLJ64_11425"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HNC94134.1"
                     /translation="MSLRCVYTDLDGTLLGAGASLFTDEEGNFSMAQARALEAASRAG
                     VEVVIMSGRRRAQVHEDARIIGQNSFIFDAGAAFSIDQETTLLLGDFELEEGQTPVQR
                     MSERGVPDLLLENFAGRFEYHDPWHLDREMSHLFRGDIDTAEANALLEENGHGDLRLL
                     DNGAIHRRMEGIEQAHCYHLVPKAVSKVNAVAAHARARGYSPEETIAVGDSLEDLEVA
                     PAVGKFFIVANGPEKDPGVREAMSRYPNAVVTEASNGAGFYEAVVSSLMGA"
     gene            2938..>3504
                     /locus_tag="PLJ64_11430"
     CDS             2938..>3504
                     /locus_tag="PLJ64_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNC94135.1"
                     /translation="MADATVAEEEYLQTIFWLMEAGLPITGANIAKAMQLSPPTVHEM
                     IKRLERDGYVTRSADKILHLTEDGRAEAEQITRRHRMIERFLTDVLGIPWDEVHEEAE
                     RLEHAMSPVLEERMRAAIGEANTCPHGHPIDGVREEGAPLADVDLGAKVVVLRFENEA
                     ESLLHYLMDIGLYPGLSGTVTAKDDESVL"
BASE COUNT          578 a         1165 c         1216 g          545 t
ORIGIN      
        1 cctcgaaggc ctcggcctga tggctgtaaa gctgttcgat gccgccccgg ttcagggctt
       61 cggcgaggcc gggatgaagc gaagccggca gcggttccag ccgggcttcg gcggccggtt
      121 cggaggcgct ctcgacgatc cggccatccc ggcgggccgg gtcgatcgcg atcgaccagc
      181 gcccggaaag cagggactca gacatcttcc ggcgaggtgg cggccggatt cggggagccg
      241 gccgcgtctc ggggcggttc gcggcggatt tcgtcggcga tcctgagggc cggatcaact
      301 tcgctttcac cgcgcagcgc ccgccagacc tggaggaagt cgcgggtacc ggccacgtcg
      361 tgaacccgga gcaggcggcc gccgcaatcg aggccgaagg cgagggcggc gaaggtgccc
      421 ggggcccgct cggaggggcg acgatgattg atcgcgccga tgaagtcctt gcgggagacg
      481 gccagcatca ccggacagcc gaggccgcag agttcgggca ggccgcgcag tgaatcgacg
      541 gtctgggccg gggtcttgcc gaaatcgggg cccggatcga ccaccacctg atcacgccgg
      601 atgccgattt cggccaggct ttccagcctt tcctcgaaga agcggacctg gtgggcggtg
      661 attccctccg ggtaggccgc ttcatcccag cgctgggttt tcggctcaac cacggtgtgc
      721 atcaggacca cacccgcccc ggcttccgcc gcgaccccca tcagttccag gttgccgccg
      781 ccgctgatgt cgttgatgat cgccgcgccc gattccaggg cagcggccgc gaccgccggc
      841 ttgtaggtgt caaccgagat gatcgccccc gccgcggcgg cggcaaccac caccggcagg
      901 attcgttcga tctcctcctc cggggtcacc gcgggccggt tggtcacgtt cgactcgccg
      961 ccgatctcga tgatgccggc cccgtcttcg aggtgctgct cgacctggcg gatcgacccg
     1021 gcggtcgagc ggtcaccgcg atcggagaac gagtccggag tggcgttgac cacgccgacg
     1081 atgaccggct cggccacgtt gatctcgcct gcggcggtgc gtagtgttcc cgggagagaa
     1141 gccatcgcca cgatgctaga caccttccac cacggggact tcgccgccgc cgacatcagc
     1201 cgcaagctgc gggaaacggt cagcgtggtg atccctgccc ggaacacggc ggggacgatc
     1261 gcggcaaccg tggccgagct tcaggtgctg gccgaggacg ggctggtcgg gcagatcctc
     1321 gtggtcgatg ccgactccgc cgacggcacc gccgcggtcg cccgggaagc cggagccgag
     1381 gtggtttcgg aaaacgaact ggtcgagggt ttcgggccct gccggggcaa gggtgacgcg
     1441 atgtggcggt cgctcgccgc ggtgaccggg gagatcgtcg tcttcatcga cggggacatt
     1501 gccgacttcg gccgccacta cgtgaccggc gttctcggcc cgctgaccga tcccggcaag
     1561 catttcgtca agggcaggta ccagcgcccg ttccggttcg gcgaaaacga ggagccggcc
     1621 ggcggtggcc gggtcaccga gctcgccgcc cggcccctgc tggcccggct cgcaccggag
     1681 ctgctcgcct tcgaccagcc cctggccggc gaggtggcgg cccgtcgcga gctgctcgat
     1741 caggtcccgt tcgcctgcgg ctacggagtc gagatcgcga tgctgatcga agtctggggc
     1801 cgggtcggca tccgtggcat ggcccaggtc gagctcggca ccaagcggaa cgcgcaccag
     1861 tcgctggccg ccctttccgg catgtcctcc gaggtgatcg ccgcgatggc cgacaccctg
     1921 gaccggctcg gccggaaaga cacggggcgc atgaagatgg tcgaaggaag cggcccggaa
     1981 gtggtgaccc gacccgcctt cgcgacggtg ccgcaggttt cgcaccgcag cggtcgcaaa
     2041 cctgcggcac cgtagtcctg tctgaactcc acttcggtgc ctcagcggat agcgtgaggc
     2101 gcgatgagtc tccgctgcgt ttacacagac ctcgacggca ccctgctcgg ggccggtgcc
     2161 tcgctcttca ccgacgagga gggcaacttc tcgatggccc aggcccgggc gctcgaggcc
     2221 gcttcgaggg ccggggtgga ggtcgtgatc atgtccggcc gccgccgggc ccaggtccat
     2281 gaagacgccc ggatcatcgg ccagaactcg ttcatcttcg atgcgggcgc ggccttctcg
     2341 attgaccagg agacgaccct gctgctcggc gacttcgaac tcgaggaggg ccagactccg
     2401 gtccagcgga tgagcgagcg cggggtcccc gatttgctgc tcgaaaactt tgccggacgc
     2461 ttcgagtacc acgatccctg gcacctcgac cgggagatgt cacatctctt ccgcggggat
     2521 atcgacacgg ccgaggccaa cgcactgctc gaggagaacg gtcacggtga cctcaggctg
     2581 cttgacaacg gcgcgatcca ccgccggatg gaaggcatcg aacaggccca ctgctatcac
     2641 ctggttccga aggcggtttc caaggtgaac gcagtcgccg cccatgcccg tgcccggggt
     2701 tactccccgg aggaaacaat cgccgtcggc gattcgctcg aggaccttga agttgccccg
     2761 gcggtcggga agttcttcat cgtcgccaac ggcccggaga aggaccccgg ggtgcgggaa
     2821 gcaatgagcc gctatccgaa tgcggtggtc accgaggcga gcaacggcgc cggcttctac
     2881 gaggccgttg tgtcatccct catgggtgcg tgaaggccaa aggtctagga tcaagccatg
     2941 gctgacgcaa cggttgccga agaggaatat ctgcagacga tcttctggct gatggaggcc
     3001 ggattgccga tcaccggcgc caacatcgcc aaggcgatgc agctctcacc gcccaccgtc
     3061 cacgagatga tcaagcggct tgagcgggac ggctacgtga cccgcagcgc cgacaagatc
     3121 cttcacctga ccgaggacgg ccgggccgag gcggagcaga tcacccgccg ccaccggatg
     3181 atcgaacgct ttctcaccga tgtgctcggc atcccctggg acgaggtcca cgaggaggcc
     3241 gagcggctcg agcacgcgat gtcgccggtg ctggaggagc ggatgcgggc ggcgatcggc
     3301 gaggccaaca cctgcccgca cggccacccg atcgacgggg tccgcgagga aggagcaccg
     3361 ctggctgatg tcgacctggg cgccaaggtg gtcgtgctcc gtttcgaaaa cgaagccgag
     3421 agcctgctcc actacctgat ggacatcggc ctctacccgg ggctcagtgg cacggttacc
     3481 gccaaggacg acgagtcggt cctg
//
LOCUS       DAOQKN010000221         3500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_16798_length_3500_cov_5.072859, whole genome shotgun sequence.
ACCESSION   DAOQKN010000221 DAOQKN010000000
VERSION     DAOQKN010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16798_length_3500_cov_5.072859"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="PLJ64_11435"
     CDS             complement(<1..351)
                     /locus_tag="PLJ64_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94136.1"
                     /translation="MPKKTETPLVMGTVSQRREPVWDPLEYMIGSRAREFMWMFEVEM
                     EDGTAVQAYKHVFTRKYFHLGSDGRSFEFRSFGGEGPSGYEQVPFLRTAGMVLWLYDQ
                     ERETLWKPGEREYCN"
     gene            complement(528..1541)
                     /locus_tag="PLJ64_11440"
     CDS             complement(528..1541)
                     /locus_tag="PLJ64_11440"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNC94137.1"
                     /translation="MLNFDVDRYLDIQSGAVNLAEGLDRTIGDLLDGGKKTLIFAGAG
                     GAGMLMQPAAQLLMRRSRFPVFWDRPAEMMAIRPVYFDENAVVIIPSLSGTTMESIEL
                     VEASREVGAVTIALTGHEDSPIAGSVDHNFTNFAEDDTSSESFYLQSLLVALSVLRRE
                     PDRVDYDSVVSELKKLPELLVDAKAAFEDRAEEWAEAFRDEDYHIISAAGPLWAEAWY
                     YGTCILEEMQWIRTRPIHAADFFHGTLELLEPEVSLILLKGSGPSRELENRVEEFAGT
                     ISNKVRVLDTDDVDMPGISDPVREMVSPAIASALLERVSAHLEVKRDHPLTTRRYYRK
                     VAY"
     gene            complement(1588..2610)
                     /locus_tag="PLJ64_11445"
     CDS             complement(1588..2610)
                     /locus_tag="PLJ64_11445"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNC94138.1"
                     /translation="MTDGRVTLQDVASLAGVSQATAGRALGGYGSISEDSRQRVEQAA
                     TKLGYRTNAVARALASGTTKSIGLVIGDIENPFFATVARGLSDVVQEAGYTVVLANAD
                     ENWDMERQALEVFDTRSVDGLVVAPAPGSDASSIEKLGNRIPLVVIDRDLPGLDVDSV
                     LVDNVAGAASATRHLVNLGHREIGLVTEPPVISSVRERIQGYEQVLEENGLATSPSLR
                     AEGTPRLEGGLLAATELLNSPDRPHAIFATTNTMTAAVLAAAAELGLKIPEDLSLVGF
                     DDSEWMRLVSPPITVVRQPVYALGQATGRLLNDRINGWTGDPKQLRLKTELVVRESCR
                     KWQEPS"
     gene            complement(2628..>3500)
                     /locus_tag="PLJ64_11450"
     CDS             complement(2628..>3500)
                     /locus_tag="PLJ64_11450"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNC94139.1"
                     /translation="EAGLPDGVLNVVPGTGEKAGRSLGLHMDVDAITFTGSTSIGKAF
                     LAYSSESNMKGVWLECGGKSPNVVLADAPDLDRAAEFAAETIFHGAGQVCNAGSRLIV
                     ERPIYEDFVSRVARESLKWAPADPLDASAKMGPLVDRPALDRALGYVSAAKSDGATVV
                     SGGGETLEATGGYFMEPTILGSVDNSMPVAQEEIFGPVLAAIELEERGEATRLANGSR
                     YGLAAAIWTSDFGRAHRVARALKAGTVYINCYDRGDNSLPFGGFKESGIGVDRSLAAM
                     EKYSNLKTIWADIS"
BASE COUNT          636 a         1106 c         1071 g          687 t
ORIGIN      
        1 gttgcagtac tcacgttcgc cgggtttcca aagagtctcc cgctcctggt catacagcca
       61 aagcaccatt ccggctgtcc tcaggaaggg aacctgctca taccccgagg gcccttcgcc
      121 cccaaagctt cggaactcga acgatcgacc gtcactcccc aggtggaagt actttcgcgt
      181 gaagacgtgc ttgtaggcct ggaccgccgt cccatcctcc atctcgacct cgaacatcca
      241 catgaactcg cgggctcgcg agccaatcat gtattcgagg ggatcccaga cgggctctcg
      301 ccgttgggag acagtaccca taacaagtgg tgtttcggtc ttcttcggca tgccccgacc
      361 atgcgcgaaa aggtgtgacg aaacaagact tcatagcaac aattgggtga cgaaatggag
      421 acgaaagcgc agcagacggg cgaaatgcgc ggggaaactc gatccagctt ctcgccttcg
      481 attcgctcac tgggtgattc aatccacgtc ctggttctca aagggcttca gtaggcgact
      541 ttcctgtagt agcggcgggt agtcagtgga tgatcccgct tgacttcgag gtgggcgctg
      601 acccgttcca gcaacgcgga ggcaattgcg ggtgagacca tttcccgtac ggggtccgat
      661 atccccggca tatcgacatc gtccgtgtcc aggactcgta ccttgtttga gatcgtcccg
      721 gcaaattcct cgaccctgtt ctcgagttcc cgagagggtc cgcttccctt cagcaggatc
      781 aagctgactt cgggctcgag caactcgagc gtgccgtgga agaaatccgc tgcgtgaatc
      841 ggccgggtcc ggatccactg catctcctcg agaatgcagg tcccgtagta ccaggcttcc
      901 gcccagagcg gcccggcggc ggaaatgatg tggtaatcct cgtcgcgaaa ggcttcggcc
      961 cactcctcgg cgcgatcttc gaaagcggcc tttgcgtcaa cgagcagttc gggaagcttc
     1021 ttcagttcgc tgacgaccga gtcgtagtca accctgtctg gctcccgccg gagcaccgaa
     1081 agcgcgacaa gcagggactg gagatagaac gactccgacg aggtgtcatc ttcggcgaag
     1141 ttggtgaagt tgtgatcgac cgacccggcg atgggtgaat cctcgtgacc ggtcagggcg
     1201 atagtgacgg cgccgacctc ccgactggct tcgaccagtt cgatgctctc catcgtggtc
     1261 cctgacagtg aggggatgat cacgaccgcg ttctcgtcga agtaaacggg cctgatcgcc
     1321 atcatttcgg ctggccggtc ccagaagacc gggaagcggg atcgccgcat aagcagttgc
     1381 gccgccggct gcatcagcat cccggcccct cccgctccgg cgaagatcaa ggttttcttg
     1441 cccccgtcca gcaggtcgcc gattgtccgg tccagtcctt cggcgaggtt gaccgcgccg
     1501 gactggatat ccaggtatct gtctacatcg aaattaagca tcattcacct tgttcatgcg
     1561 catgttgatc ggggttattg acctgggtca ggaaggctcc tgccacttgc ggcaggattc
     1621 ccggacgacc agttcggttt tcaggcggag ttgtttcgga tctcccgtcc agccgttgat
     1681 ccggtcgttc agcagccgtc cggttgcctg gcccagcgca tagaccggtt gccggaccac
     1741 ggttattggc ggggacacga gccgcatcca ctccgaatcg tcaaacccga cgagcgacag
     1801 gtcttcggga atcttcagtc cgagttccgc cgccgccgcg agaaccgctg cggtcatcgt
     1861 gttggtggtg gcgaaaatcg cgtgggggcg atcgggagag ttcagcagtt cagtggcagc
     1921 gagcaggccg ccttcaagtc tgggcgtgcc ttccgctcgt aaggacgggg aagtcgctaa
     1981 accgttttcc tcgagcacct gctcgtagcc ctggattcgc tcgcgaaccg acgagatgac
     2041 gggcggctcg gtaaccagcc cgatttcgcg gtggccgaga ttgaccagat gtcgggtcgc
     2101 gctggcggcc ccggcaacgt tgtcgaccag caccgagtcg acgtcaagtc cgggcaggtc
     2161 gcggtcgatc acgaccagtg gaatccggtt gcccagcttc tcgatcgaac tcgcgtccga
     2221 accgggcgcg ggagcgacga ccagaccgtc gaccgagcgg gtgtcgaaaa cctcgagcgc
     2281 ctgacgttcc atgtcccagt tttcgtcggc gtttgccaga accacggtgt agcccgcttc
     2341 ctgaactaca tcggaaagtc cgcgggccac agttgcgaag aacggatttt cgatgtcgcc
     2401 gattaccagc ccgatgctct tggtcgtgcc cgaggcgagc gcccgggcga cggcgttggt
     2461 tcggtacccc agcttggtcg ccgcctgttc gacgcgctgg cgggaatcct cgctgatcga
     2521 cccgtagccg cccagtgccc ggccggccgt agcctgggaa acaccggcga gcgaggccac
     2581 atcctgaaga gtcacccgac cgtcggtcat gtttccgtct ctttcgctca tgaaatgtcg
     2641 gcccagatcg tcttcaggtt cgagtatttc tccatcgcgg cgagcgaccg gtcaacgccg
     2701 atccccgact ccttgaaacc cccgaaggga agtgaattat cgccgcggtc gtagcagttg
     2761 atgtaaacgg ttcccgcctt gagggcgcgc gcgacgcggt gggcccggcc aaagtccgag
     2821 gtccagatcg cggcggcgag tccgtatctc gagccattgg cgagtcgcgt ggcctcgccc
     2881 cgttcttcca gctcgatcgc ggcgagcacc gggccgaaga tctcctcttg cgcaaccggc
     2941 atcgagttgt cgaccgagcc gagaatcgtc ggttccatga agtacccccc tgtggcctcg
     3001 agggtctcgc ctccgccgct gaccacggtc gccccgtcgg actttgcggc ggacacgtaa
     3061 ccgagagccc ggtcgagggc cggccggtcg accagtgggc ccatcttcgc cgaggcgtcg
     3121 agcgggtctg ccggcgccca cttgaggctt tcccgggcaa ctcgacttac gaagtcttca
     3181 tagatcggtc gctcgactat gagtcgggat cccgcgttgc agacctgacc ggcaccgtgg
     3241 aagatcgtct ccgcggcaaa ctctgcggcc cggtcgaggt cgggcgcatc ggccagcacc
     3301 acgttgggag acttcccgcc gcattccagc cagacgcctt tcatgttcga ctcggatgaa
     3361 taggcgagga aggccttgcc gatcgaagtc gaaccggtga acgtgatcgc gtcgacgtcc
     3421 atgtggaggc ccagcgaccg cccggccttc tcgccggttc ccggcacgac gttgaggacg
     3481 ccgtccggaa gaccggcttc
//
LOCUS       DAOQKN010000222         3481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_16965_length_3481_cov_4.403094, whole genome shotgun sequence.
ACCESSION   DAOQKN010000222 DAOQKN010000000
VERSION     DAOQKN010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16965_length_3481_cov_4.403094"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /gene="guaA"
                     /locus_tag="PLJ64_11455"
     CDS             complement(<1..910)
                     /gene="guaA"
                     /locus_tag="PLJ64_11455"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNC94140.1"
                     /translation="MTAATTEEVLVLDFGGQYSQLIARRIRECGVFAELLPASTTIEQ
                     IESRNPKGLVLSGGPASVYDPGAPEFPEKLTELGIPVLGICYGMQAMAKALGGKVEGA
                     ESGEFGRTGLTVTGDGGVLLGGLPSEQTCWMSHRDSVFEAPEGFTPIASTPGSPVAAL
                     ESAEKKLYGIQFHPEVVHTPYGTEVLKRFLREVAGCEEQWSAASVVTEQVNAIREQIG
                     EGKAICGLSGGVDSSVAAKLVHEAIGDRLTCVFVDHGLMRMNEAEQVVEDFQHFGIPL
                     VHVDAEERFLTKLAGVTDPETKRKIIG"
     gene            complement(958..2163)
                     /locus_tag="PLJ64_11460"
     CDS             complement(958..2163)
                     /locus_tag="PLJ64_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HNC94141.1"
                     /translation="MEIEIGRGKKGRRAYGFDDVAIVPSRRTRDPDDIDISWTLGPYR
                     FDLPLVASAMDGVVSPETAVLVHKLGGLGVLNLEGIWTRFEDAEEKLELIANSSKDEA
                     TALMQEIYETPVKKELIARRVAEIKAEGAVVCGSFTPQSVQENYEAALEAGLDILVIQ
                     GTVISAEHVSTTVEPLNLKEFIAEVPVPTVVGGCASYSTGLHLMRTGAVGVLVGVGPG
                     AACTTRGVLGIGVPQATAIADVAAARSQHMLETGEYVNVIGDGGMRTGGDISKAIACG
                     ADAVMIGSPLARAKEAPGRGYHWGMATFHSSLPRGTRVTTVQDGTMEEILLGPAHEND
                     GTFNLMGALRTSMATTGYEDIRSFQRAEMMIAPALMTEGKKLQTEQAVGMGSNGRAAV
                     SAGVGVADD"
     gene            complement(2549..>3481)
                     /locus_tag="PLJ64_11465"
     CDS             complement(2549..>3481)
                     /locus_tag="PLJ64_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HNC94142.1"
                     /translation="AAIDRTVAVPNLRLGHRHRSVETRTVPGGKGINVARALGLLGRP
                     VIATGLVGGGNGDRVLKQLGEEGLLTDFIRIAEETRFNLALVDPTTGEQTEVNERGPR
                     VSPEELDAFIERLDYLAGGAKLCVLAGSLPPGIDVEFYARLVEALKQAGLPVLLDSEG
                     EAMSAGMKVGPDMITPNKLEAEELVGHEFESRAEMFGVLSELVEMGPAEAAITLPEGC
                     VAIVGEGADQRMLEAVIEPLEPISTVGSGDAFVAGYIAARYDGGSPEECLAYGVACGA
                     ESTQHLGAGRIDRGRAESLLGNVEVRQLQLPAEV"
BASE COUNT          576 a         1209 c         1108 g          588 t
ORIGIN      
        1 cgccgatgat cttgcgcttg gtctcgggat cggtgacccc ggccagcttg gtcaggaagc
       61 gttcctcggc gtcgacgtga accagcggga tcccgaagtg ctggaaatcc tcgactacct
      121 gctcggcctc gttcatccgc atcagtccgt gatcgacgaa gacgcaggtc agccggtcac
      181 cgatcgcttc gtgaaccagt ttcgcggcga ccgatgaatc gactccaccg gagaggccgc
      241 atatcgcctt gccctcacca atttgctcgc ggatcgcatt tacctgctcg gtcacgaccg
      301 aagcggcact ccactgttcc tcacagccag cgacttccct caggaaccgc ttcagcacct
      361 ctgtgccata cggcgtgtgg actacttcag ggtggaactg gatcccgtag agcttcttct
      421 cggccgattc cagggcggca accggcgaac cgggagtcga ggcgatcggg gtgaagcctt
      481 cgggtgcctc gaagaccgaa tcgcggtggc tcatccagca ggtctgctcc gaaggcagcc
      541 cgccgagcag cacgccgccg tccccggtca cggtcagccc ggtccggccg aactcgcccg
      601 attcggcgcc ttcgaccttg ccgccgaggg ccttcgccat cgcctgcatt ccgtaacaga
      661 tgccgagcac cgggatgccg agctcggtca gcttctccgg aaactccggc gcgcccgggt
      721 catagaccga ggccggaccg cccgagagca ccagcccctt cggattgcgc gactcgatct
      781 gctcgatcgt cgtgctggcc ggcagcagct cggcaaagac gccgcattcg cggatgcggc
      841 gggcgatgag ctgggagtac tggcccccga aatcgaggac caatacttcc tccgttgtgg
      901 cggcggtcat cagtggacgg gaccctgact ggcggacgaa aaccggaggc cggcccctca
      961 gtcgtccgcg accccgactc ccgccgaaac ggcggcccgg ccgttcgaac ccatgccgac
     1021 ggcctgctcg gtctggagct tcttgccttc ggtcatcagc gccggcgcga tcatcatctc
     1081 ggcccgctgg aacgagcgga tgtcctcgta gccggtggtc gccatcgagg tccggagggc
     1141 acccatcagg ttgaaggtgc cgtcgttctc gtgggccggg ccgagcagga tctcctccat
     1201 cgtgccgtcc tggacagtgg tcacgcgggt accgcgcggc agcgacgagt ggaaggtcgc
     1261 catgccccag tggtagccac ggccgggagc ttccttggct cgtgccagcg gcgaaccgat
     1321 catcacggcg tcggccccgc aggcgatcgc cttcgagatg tcgccaccgg tccgcatgcc
     1381 gccgtcaccg atcacgttga cgtactcgcc ggtctccagc atgtgctgcg aacgggctgc
     1441 cgccacgtcg gcgatcgcgg tcgcctgcgg cacaccgatg ccgagcacgc cgcgggtggt
     1501 gcaggcggcg cccgggccga cgccgaccag cacgccaacc gcgccggtcc gcatcaggtg
     1561 caggccggtc gagtacgagg cgcagccacc gaccacggtc gggaccggca cctcggcaat
     1621 gaactccttc aggttgagcg gctcgaccgt ggtcgagacg tgctcggcgg agatcaccgt
     1681 gccctggatg accaggatgt caagccccgc ctcgagcgcg gcttcgtagt tctcctgaac
     1741 cgactgcggg gtgaaggatc cgcagaccac ggcgccctcg gccttgatct cggcgacccg
     1801 ccgggcgatc agttccttct tgaccggagt ctcgtagatc tcctgcatca gggcggtcgc
     1861 ctcgtccttg gacgagttcg cgatcagctc gagcttttcc tcggcgtctt cgaagcgggt
     1921 ccagatgccc tcgaggttga gcacaccgag accacccagc ttgtggacga gaactgcggt
     1981 ttccgggctg acgacaccat ccatggcgga agcgaccagg ggcaggtcga accggtacgg
     2041 gccgagggtc cagctgatgt caatgtcatc aggatcccgc gtccgtcgag aaggaacgat
     2101 ggcaacatcg tcgaatccgt aagcgcgtcg gcctttcttg ccacggccga tctcaatttc
     2161 caattaggtc ctccagggct ctgagggtga agaggggcag actacgcgcc cctgcggacg
     2221 tttgatcaga gactacgcct ctccaccctt cggcgttttc cgttgttccg caaagcggga
     2281 ggtctttggc ggcccggcac ctgcacgagc gaagccccca cacaaaagga tggaggcttc
     2341 gcctggaggg tgtgagttcc ggcttccgat cccgaaccca cgattactgc cacatgaccg
     2401 gggaggagaa gccccgtatc ccaggattca atctagaacc ggccgcccgg acgatcgatc
     2461 gaccgcggta caggaaaccg cgccggctgc tgtcccccgg tcccaccgcc catccgcacc
     2521 cggaagccgg ggggcggccg ggcgctcttc agacttcggc cggcagctgg agctgacgga
     2581 cctcgacgtt gccgagcagg ctttcggccc ggccgcggtc gatccgcccg gctccgagat
     2641 gctgggttga ctcggctcca caggcgaccc cgtaggccag gcactcctcc gggctgccgc
     2701 cgtcatagcg ggcggcgatg taaccggcca cgaacgcgtc accggacccg accgtggaaa
     2761 tgggttcgag cggttcgatc acggcttcga gcatccgctg gtcggcgcct tccccgacga
     2821 tcgcgacgca accttccggc agggtgatcg ccgcttcggc cggtcccatt tcaaccagct
     2881 cggaaagcac cccgaacatt tccgcccggg actcgaactc gtgaccgacc agctcctcgg
     2941 cctccagctt gttcggagtg atcatgtccg ggccgacctt catgcccgcc gacatcgctt
     3001 caccctcgga gtcgagcagc accggcaggc cggcctgctt cagcgcctcg accagacgcg
     3061 cgtagaactc gacgtcaatc ccggggggca ggctgccggc cagcacgcaa agcttggcgc
     3121 ccccggccag gtagtcaagc cgttcgatga aggcatcgag ttcttccggg ctgaccctgg
     3181 gaccccgttc gtttacctcg gtctgttcac cggtggtcgg atcgaccagg gccagattga
     3241 agcgggtctc ctcggcgatc cggatgaaat cggtgagcag gccttcctcg ccgagctgct
     3301 tcagcacccg gtccccgtta ccgccaccga ccagtccggt cgcgatgacc ggccggccga
     3361 gcagcccgag ggcgcgggcc acgttgatgc ccttgccgcc gggcacggtg cgggtttcaa
     3421 ccgaacgatg ccggtgaccg agccgcagat tgggtacggc gaccgtgcgg tcgatcgccg
     3481 c
//
LOCUS       DAOQKN010000223         3477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_17002_length_3477_cov_4.809760, whole genome shotgun sequence.
ACCESSION   DAOQKN010000223 DAOQKN010000000
VERSION     DAOQKN010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17002_length_3477_cov_4.809760"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="PLJ64_11470"
     CDS             <1..956
                     /locus_tag="PLJ64_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94143.1"
                     /translation="NLESNFLPQKRSSGKSSPVLYRSVLNRFAAAVKAVDPSNLVVGG
                     GLSPLGGGKSYHPLAFARQLLCMQGRARPTPIRGCSGTARFDIWASNPYTTGGPTHKS
                     TNPDDIQLGDLSSMSRLLRAARKAGKIRSSFKSIPYWLTEFSWDSNPPDPGGVKMPLL
                     KRWTAHAMFQAWRSGVSAFFWFSLRDIERTQGHPYAWTLESGLYQRSNSLSNDQPKGN
                     LTAFRFPVVAFRRPGGIYFWGRNPGGKSGKAVIRFRPPNGSGSRVLKRIKVGSGGVFQ
                     GFVRTRLGRNFKGSVFADSGGQASVPFSLTTVKDFYQPPFG"
     gene            1009..2061
                     /locus_tag="PLJ64_11475"
     CDS             1009..2061
                     /locus_tag="PLJ64_11475"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNC94144.1"
                     /translation="MITGGAGFVGSSLAISLAESHPDRRIVALDNLYRRGSELNLDRL
                     GQAGVEFVKGDVRNPADLAPFEDIDALIECSAEPSVMSGTDGDTSYVYETNLTGAYNC
                     LELARKSGCQFIFLSTSRIYPMVGLREAALVEGETRFELAEDQPSPGWTSAGVSERMP
                     LEGARTFYGTTKLSAEFLCAEFAAQFGVPVVINRCGVIAGPWQMGKVDQGVFTHWMLA
                     HEFEKPLSYIGFGGSGKQVRDLLHVDDLVELIEEQLADPDHWAGQTFNVGGGREVSLS
                     LLEATEICGRLTGHELAIGAVPEDRDGDVPLYISDCSRLFAHTSWRPKRDAECVLADI
                     HTWIGSDRERVGAVLG"
     gene            2073..2801
                     /locus_tag="PLJ64_11480"
     CDS             2073..2801
                     /locus_tag="PLJ64_11480"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNC94145.1"
                     /translation="MIPARDEEGSIMKTVDGVVEVMERESLEYEVLVIDDGSKDATRK
                     LVEAAGESNANIRCRPSPYSGGFGLAVRAGLEQFEGDGVAIMMADCSDDPEDLVMYHH
                     ILAEGWDCAFGSRFMPGAVVTDYPRLKLLVNRMANAFVNVLFRLGYNDTTNAFKAYRR
                     EVIETIGPLLSKHFNLTVEMPLKAVTRGHSFKVVPTSWTNRTAGEAKLAMREMGSRYL
                     FIVLYIWLEYHLSRGDYRKAEGSG"
     gene            2798..>3477
                     /locus_tag="PLJ64_11485"
     CDS             2798..>3477
                     /locus_tag="PLJ64_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94146.1"
                     /translation="MTSGGSTFRDWLGRTEEVNPRSWLVGLVVALLGWQVSLQAPVYG
                     LDPSWWSGLYMASHEKMRFGTEIVFTYGPLGILREPWLFYPGLLSLIPFLYKAALFTG
                     FCIALVAVLRRRIGWLFAALAALLLLLLLRQIELAVAISMLVSILIIERSPDDRVMPF
                     LAIGGGLLAGIEMLGKLSAGPVVAVVLLLGLIGAHARMRDLIAYGAALVVSMGAFWLV
                     TGQSLGDLP"
BASE COUNT          552 a         1140 c         1125 g          660 t
ORIGIN      
        1 ccaacctcga atcaaacttc ctgccgcaga agcgcagctc gggcaagtca tccccggtgc
       61 tctaccgatc ggtactgaac cgtttcgcgg cggcggtcaa ggccgtggac ccgtccaacc
      121 tcgtggtcgg cggcggtctc tctcccctcg gtggcggcaa gtcctaccac ccgctcgcct
      181 tcgccaggca gctgctctgc atgcaggggc gggccaggcc gaccccgatc cggggatgct
      241 ccggcacggc ccgcttcgac atctgggcct ccaacccgta cacgactggc gggcccaccc
      301 acaagtcgac caaccctgat gacatccagc tcggcgatct ctcgagcatg agtcggctgc
      361 tacgcgcggc ccgcaaggcc ggcaagatca ggtcgtcctt taagagcatt ccctactggc
      421 tgaccgagtt cagctgggat tcgaacccgc cggatccggg cggagtcaag atgccgctgc
      481 tcaagcgctg gactgcccac gcgatgttcc aggcctggcg ctccggcgtc tcggccttct
      541 tctggttctc gctgcgggac atcgagcgga cccaggggca cccctacgcc tggaccctcg
      601 agtcgggtct ctatcagcgc agcaactcgc tctcaaacga tcaaccgaag ggcaacctga
      661 cggcattccg cttcccggtg gtcgccttcc gtcgtcccgg cgggatctac ttctggggcc
      721 gcaaccccgg tggcaagtca ggcaaggcgg tgattcgctt ccggccaccg aacggctccg
      781 gcagccgcgt cctgaagcgg atcaaggtcg gcagcggcgg cgttttccag ggcttcgtgc
      841 ggacccgcct cggtcggaac ttcaagggct cggtcttcgc cgactccggc ggtcaggcct
      901 cggttccctt ctctctcacc accgtcaagg acttctacca gccgcccttc ggataaaact
      961 ccggattcca ttgtgaatct ggacgcgcta cccgggggga aatccatctt gattacgggc
     1021 ggagccggct ttgtcggttc cagccttgcg atctcgctgg ctgagtcgca tcccgacagg
     1081 cggatcgtcg cgctggacaa cctttatcgc cgcggctccg agctgaacct cgaccggctg
     1141 gggcaggccg gggtcgagtt cgtcaagggt gacgtgcgga acccggccga cctcgcaccc
     1201 ttcgaggaca tcgacgccct gatcgaatgt tcggccgaac catcggtcat gtcggggacc
     1261 gacggtgaca cttcgtatgt ctatgagacg aatctgaccg gcgcctacaa ctgcctggag
     1321 ctggcgcgga agagcggttg ccagttcatc ttcctttcaa ccagccggat ctatccgatg
     1381 gtcgggctgc gtgaagcggc cttggtcgag ggggagaccc ggtttgagct ggcagaggac
     1441 cagccttcac ccggatggac ttcggccgga gtctccgagc gcatgccgct ggagggtgcg
     1501 cgcaccttct atggcacgac caaactctcg gccgagttcc tctgtgccga gttcgcggcc
     1561 cagttcgggg tgccggtggt gatcaaccgc tgcggggtga tcgccggtcc ctggcagatg
     1621 ggcaaggtcg accagggggt gttcacccac tggatgctgg cccacgaatt cgagaagccg
     1681 ctctcctaca ttggcttcgg cggcagcggt aagcaagtgc gggacctgct ccacgtcgac
     1741 gacctggttg aactgatcga ggagcagctg gctgacccgg atcactgggc cgggcagacc
     1801 ttcaacgtcg gcgggggccg ggaggtcagc ctttcgctgc tcgaggcgac cgaaatctgt
     1861 ggccgcctga ccggtcacga gctggcgatc ggcgccgtgc cggaagaccg cgacggtgac
     1921 gtgccgcttt acatctcgga ttgctcccgg ctcttcgccc atacctcgtg gcgaccgaaa
     1981 cgcgacgccg aatgcgtgct ggccgacatc cacacctgga tcggttcgga ccgtgaacgg
     2041 gtcggggcgg tgctgggatg aggctctcgg tcgtgatccc cgcccgggac gaagaaggct
     2101 cgatcatgaa gacggtcgat ggcgtggtcg aggtgatgga gcgcgaatca ctcgagtacg
     2161 aagtgctggt gatcgacgac ggcagcaagg acgcgacccg caagctggtc gaggcggccg
     2221 gcgagagcaa tgccaacatc cgctgccgcc cttcgcccta ttccggcggg ttcggactgg
     2281 cggtgcgggc cggcctcgag cagttcgagg gtgacggggt ggcgatcatg atggccgact
     2341 gttccgatga tccggaggac ctggtcatgt accaccacat ccttgccgag ggttgggatt
     2401 gcgcctttgg ctcgaggttc atgcccggtg cggtggtgac cgattacccc cggctcaagc
     2461 tcctggtcaa ccggatggca aatgccttcg tcaacgtgct cttccggctg gggtacaacg
     2521 acacgaccaa cgccttcaag gcctaccggc gcgaggtcat cgagacgatc ggcccgctgc
     2581 tttccaagca cttcaacctg accgtggaga tgccgctcaa ggcggtcact cgcgggcaca
     2641 gcttcaaggt cgtgcccacc tcctggacca accgcaccgc cggtgaagcg aaactggcga
     2701 tgcgcgagat gggcagccgc tacctcttca tcgtgctcta catctggctc gaataccacc
     2761 tgagccgagg cgattaccgc aaggccgaag ggtctggttg acctccggcg gatcgacttt
     2821 ccgggactgg ctgggccgga ccgaggaggt aaatccccgc agttggctgg tcggtctggt
     2881 ggtcgcgctc ctgggctggc aggtcagtct ccaggcaccg gtatacggac ttgacccgag
     2941 ctggtggtcc ggtctctaca tggcctccca cgagaagatg cggttcggca ccgagatcgt
     3001 cttcacctac ggtccgctgg gaatcctgcg ggagccctgg ctcttttatc cgggcctgct
     3061 cagcctgatc cccttcctct acaaggcggc cctcttcacc ggattctgca ttgccctcgt
     3121 cgccgtgctg aggaggcgga tcggctggct tttcgccgcg ctcgccgcgc tcctgctgct
     3181 gctcctgctc cggcagatcg aactggcggt ggcgatttcg atgctggttt cgatcctgat
     3241 catcgagcgg tcccccgatg accgggtgat gcccttcctg gcgatcggcg gcggcctgct
     3301 ggccggcatt gagatgctgg gcaaactctc ggccggaccg gtggtcgccg tcgtgctgct
     3361 gctcggtctg atcggggctc acgcgaggat gcgcgacctg atcgcctacg gggccgccct
     3421 ggtcgtctcg atgggggcct tctggctggt gaccggccag tccctcggcg acctgcc
//
LOCUS       DAOQKN010000224         3464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_17110_length_3464_cov_5.371077, whole genome shotgun sequence.
ACCESSION   DAOQKN010000224 DAOQKN010000000
VERSION     DAOQKN010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17110_length_3464_cov_5.371077"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="PLJ64_11490"
     CDS             <1..300
                     /locus_tag="PLJ64_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94147.1"
                     /translation="PPLWHESLEIVRAWQPERIAFQHFGQATDVEHQIESMHESLDLF
                     AARARETDAGGLAAWIREWLSGQVDDETLDTYWRAGPFEGMWSGLDRYWQKQEES"
     gene            complement(306..1220)
                     /locus_tag="PLJ64_11495"
     CDS             complement(306..1220)
                     /locus_tag="PLJ64_11495"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC94148.1"
                     /translation="MSAADLRDWRDRGRMVPATPLGHEAFLIDEGNPEAPPESTVVLL
                     HGFPESSYSYSGAFPLVSGAFDRVVSFDFIGFGFSAKPADGFAYSLIEQADVALRLIR
                     GSGITGCHFVAHDMGDSVMTEILARLNENRPDWFRDGALSVSFTNGSMVLGDAKLRVG
                     QRALVSPAGRMLSRIMDSPKIFEKQVVSAQGNDRLTEEQILDMYSASTSGAPRGLMAE
                     LIGYLRERRRFEKSRWLPAVRDGAMPIQICWGADDAVAPVAIAHRLKTEVKPSAKLTI
                     VDGLGHFAQIQDPEAWFNGLSPFWSEIA"
     gene            complement(1217..1489)
                     /locus_tag="PLJ64_11500"
     CDS             complement(1217..1489)
                     /locus_tag="PLJ64_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HNC94149.1"
                     /translation="MCRNIRTLYNFEPEATDEEVRAASLQYVRKISGFNKPSRANEEA
                     FDRAVEEIAAVSARLLDSLETSAPPKDRETEERKRRERAAKRFAAA"
     gene            complement(1525..1701)
                     /locus_tag="PLJ64_11505"
     CDS             complement(1525..1701)
                     /locus_tag="PLJ64_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HNC94150.1"
                     /translation="MTDHKTDEAKGRVKEAAGAITDDDELKNEGKADRAASSVKEKVE
                     DAVDTVKEKLAGKD"
     gene            1843..3213
                     /locus_tag="PLJ64_11510"
     CDS             1843..3213
                     /locus_tag="PLJ64_11510"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HNC94151.1"
                     /translation="MSDYAVVNPATGETVKEYPTISDAGLQEAIASAEDAFRDWSKNS
                     SIEERAAAVRRVGELHVERREELADIIIREMGKPREQALGEIDFCGDIYGFYADNAEK
                     LLADEPIELLAGEGDALIRKSAMGPLIGIMPWNFPYYQVARFAGPNLTVGNPVLLKPA
                     PQCPESAEAIAKMMDDAGVPSGAYQTILATNDQIADAIADPRVRGVSLTGSERAGAAV
                     AEIAGRNLKKVVLELGGSDPFILLGSGDLEEAIQSAVDARLDNTGQSCNAAKRFIVTE
                     DLYDDFLAGFTEKMKSVEPGDPASDETAIGPLSSSTATDRLQDQLDRAVAQGAEVVVG
                     GERNGNFFPVTVLTGVTPENDAYREEFFGPVATVYKVASEDEAVELANDTPFGLGSYV
                     FTDDPEQARRVAENLDTGMVYVNGVGLDGAELPFGGVKRSGFGRELGTYGIQEFINKK
                     LIRTLK"
     gene            complement(3215..>3464)
                     /locus_tag="PLJ64_11515"
     CDS             complement(3215..>3464)
                     /locus_tag="PLJ64_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94152.1"
                     /translation="GPGTLVMARGYPLTAGDEVEAAVEVEALVAILAEQGEMSREELA
                     RKAGARQWGPGRFGYVLAQAVAGGRVVRSGRRVSLPSG"
BASE COUNT          605 a         1173 c         1092 g          594 t
ORIGIN      
        1 ccgccactct ggcacgagtc gctcgagatc gtgcgagcct ggcagcccga acgcattgcc
       61 tttcagcatt tcggacaggc aaccgacgtc gagcatcaga tcgaaagcat gcacgaatcc
      121 ctcgacctct ttgcagcccg ggcccgggaa accgatgccg ggggccttgc ggcctggatc
      181 cgcgagtggc tatcggggca ggtcgatgac gaaacgctgg acacctattg gcgggccggg
      241 cccttcgaag gaatgtggtc ggggctcgac cggtactggc agaagcagga agagagctag
      301 gtcgctcagg cgatttcgga ccagaaggga gaaagcccgt tgaaccaggc ttcgggatcc
      361 tggatctggg cgaaatgtcc gagaccgtcg acgatcgtca gcttcgcgga aggtttcacc
      421 tcggtcttca gccggtgggc gatcgccacc ggggccaccg catcgtcggc tccccagcaa
      481 atctggatcg gcatcgcccc gtcccggacc gccggcagcc accgtgactt ctcgaaacgg
      541 cggcgctcgc gcaggtaacc gatcaactcg gccatcagcc cgcgcggcgc acccgaggtc
      601 gaggccgaat acatgtcaag gatctgttct tcggtcaggc ggtcgttgcc ctgggcgctt
      661 acgacctgct tctcgaagat cttcgggctg tccatgatcc gggagagcat tcgccccgcc
      721 ggcgagacca gtgccctctg cccgacccgc agtttggcgt ctcccagcac catgctgccg
      781 ttggtgaagc tgacgctgag cgccccgtcc cggaaccagt ccggccggtt ctcgttcagg
      841 cgggcaagga tctcggtcat caccgagtcc cccatgtcat gcgccacgaa gtggcagccg
      901 gtgatgcccg acccgcggat caggcgcagg gcgacatcgg cctgctcgat cagcgaatag
      961 gcaaatccgt cagccggctt ggccgaaaag ccgaagccga tgaagtcgaa cgagacgacc
     1021 cgatcgaagg ccccggagac cagcgggaag gcacccgaat acgagtacga ggattccggg
     1081 aagccgtgca gcagcaccac ggtcgattcg ggcggggctt ccgggttccc ctcatcgatc
     1141 aggaaggcct cgtggcccag tggcgtcgcc gggaccattc ggccacggtc acgccagtcc
     1201 cgcaggtcgg cggcgctcac gctgcggcga accgttttgc ggcccgctcc cgccgcttcc
     1261 gctcctcggt ctcgcggtcc ttgggcggtg cggaagtctc gagcgaatcg agcagcctcg
     1321 ccgagacggc ggcgatctct tcgaccgccc ggtcgaacgc ttcctcgttg gccctggagg
     1381 gtttgttgaa cccgctgatc ttcctcacgt actggaggga ggcggcccgc acttcttcgt
     1441 cagtcgcctc gggttcgaag ttgtaaaggg tcctgatatt ccggcacatg ccccgatctt
     1501 acgccccgcc gcgggggcgt cgggtcagtc cttgccggcc agtttctcct tgaccgtgtc
     1561 caccgcgtct tccaccttct ccttgaccga cgaagcggcc cggtcggcct tgccttcgtt
     1621 cttgagctca tcgtcgtcgg tgattgctcc cgcggcttcc ttcacgcggc ccttggcttc
     1681 gtctgtcttg tgatcggtca ttgctacctc cttctgggtt tcaaccaggt agtaccccgg
     1741 tgctcgcgct ccaatccgat tcacccgaaa cggccaacca atagtctgcg gtgagacacc
     1801 ttcaggtgtc agccaaaggc gcaaatcgag gattggagat tcatgagcga ctacgcagtt
     1861 gtgaacccgg caacaggcga aaccgtgaag gagtatccga cgatctcgga cgccggcctc
     1921 caggaagcga tcgcttccgc cgaggatgcc ttccgggact ggtcgaagaa cagttcgatc
     1981 gaggaacggg ccgcggcggt gcgccgggtc ggcgagcttc acgtcgagcg ccgtgaggaa
     2041 ctggccgaca tcatcatccg cgagatgggc aagccgcgcg agcaggctct cggggagatc
     2101 gacttctgtg gcgacatcta cggcttctac gccgacaacg ccgagaagct tctggccgac
     2161 gagccgatcg aacttctggc cggtgaaggc gacgccctga tccgcaagtc cgcgatgggt
     2221 ccgctgatcg ggatcatgcc ctggaacttc ccctactacc aggtggcccg ttttgccggc
     2281 ccgaacctca cggtcggcaa ccccgtcctg ctcaagccgg ccccgcagtg tccggaatcg
     2341 gctgaagcga tcgcaaagat gatggatgac gccggggttc cgtccggggc ctaccagacc
     2401 atccttgcga ccaacgacca gattgccgac gcgatcgccg atccccgggt ccgcggcgtg
     2461 tcgctgaccg gctccgaacg ggccggcgcg gccgtcgccg aaatcgccgg ccgcaacctg
     2521 aagaaggtcg tgctcgagct cggcggttca gaccccttca tcctgctcgg ctccggggat
     2581 ctcgaggaag cgatccagtc ggcggtcgac gcccggctcg acaacaccgg gcagtcatgc
     2641 aacgccgcca agcgcttcat cgtgaccgag gatctctacg acgacttcct cgccggcttc
     2701 accgagaaga tgaagtcggt cgagccgggt gatccggcct cagatgaaac cgcgatcggg
     2761 ccgctttctt ccagcaccgc gaccgaccgg ctccaggacc agcttgaccg cgcggtcgcc
     2821 cagggcgccg aagttgtcgt cggcggcgaa cggaacggga acttcttccc ggtcaccgtg
     2881 ctgaccggag tcacccccga gaacgacgcc taccgggaag agttcttcgg gccggtggcc
     2941 accgtctaca aggtggcgag tgaagacgaa gcggtcgaac tcgccaatga caccccgttc
     3001 gggctgggtt cgtacgtctt caccgacgat ccggaacagg cccggcgggt agccgagaac
     3061 ctcgacaccg gcatggtcta tgtgaacggg gtcggcctcg acggcgccga acttcccttc
     3121 ggtggcgtca agcgttccgg tttcggccgc gagctgggca cctacggcat ccaggaattc
     3181 atcaacaaga agctgatccg gaccctcaag tagttcagcc ggagggcagg ctcacccggc
     3241 ggccgctgcg caccacgcgg ccgccggcca cagcctgggc gagcacgtag ccgaaccggc
     3301 cgggacccca ttgcctggcg cccgccttcc tggccagttc ctcgcggctc atttcgccct
     3361 gctcggccag aatcgcgacc agcgcctcca cttcaaccgc ggcctcgacc tcgtcaccgg
     3421 cggtaagcgg gtagccacgg gccatcacga gcgtgcccgg gccg
//
LOCUS       DAOQKN010000225         3431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_17415_length_3431_cov_4.880332, whole genome shotgun sequence.
ACCESSION   DAOQKN010000225 DAOQKN010000000
VERSION     DAOQKN010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17415_length_3431_cov_4.880332"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="PLJ64_11520"
     CDS             complement(<1..769)
                     /locus_tag="PLJ64_11520"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HNC94153.1"
                     /translation="MAASGASAAEALAAAIDAFTAAGIETPRLDAEVLLAELAGLDRA
                     ALIADRELILEPAAARAYSEAVRRRLRREPVAYILGRKGFRHIDLAVDRRVLVPRPET
                     EMLVELAREVNPDTVLEIGTGSGAIALAVADELAACQVTATDVSDGALEVAIANAGRL
                     GLGERVRFTLGSLPEGPAGFDLILANLPYVPDSDGLPPEVGQWEPAGALFGGPDGTRV
                     IAEVLAGLGEAGIGAPVIGLEIGQGQGELVAGMVRDAG"
     gene            complement(772..>1820)
                     /gene="prfA"
                     /locus_tag="PLJ64_11525"
     CDS             complement(772..>1820)
                     /gene="prfA"
                     /locus_tag="PLJ64_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006303831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNC94154.1"
                     /translation="AEQIEARFAELEQQMSDPEVIGDRERYAEVGREYRQIQPANALV
                     TEYRTLSDDLEGARELLEEGEDEDLEKVVREAPARLEELEEEIRLAMVEPDPNDDKNV
                     IVEIRAGTGGDEAALFAGDLYKMLTRYAAERGYSTEVLSQSAAEQGGFKDVTFEVRGA
                     GAYSVYKYEGGTHRVQRVPETESQGRIHTSTATVAVLPEAEEVEVDIDQNDLQIDVYR
                     SSGPGGQSVNTTDSAVRITHQPTGIVVSMQDEKSQLQNREKAMRVLRARLYERQLAEQ
                     QAAVAAERKAQVGTGERSEKIRTYNFPQGRVTDHRIKLTAHDLDGIMNGNLNQFTDAL
                     AAEEKRQRLEAQAV"
     assembly_gap    1821..1920
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1921..2800)
                     /locus_tag="PLJ64_11530"
     CDS             complement(<1921..2800)
                     /locus_tag="PLJ64_11530"
                     /inference="COORDINATES: protein motif:HMM:NF018798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HNC94155.1"
                     /translation="MQSNGASPTRSGAVSPAPLEAKRDAPVGGQAVLEGVMMRGVTTW
                     AVSVRKPDGEIETTTEKIEPWARRHRVLRWPIIRGIVALAESLKIGFRALGMSANAQL
                     DEGEEEEELGGMAWGMTIFVSLAFAILLFFVIPVGLTSLIKDWLDSSFLFWLVEGIVR
                     TAIFIGYIVMISRLEDLRRVFEYHGAEHKTISCYEAEDELIPSRAALYSRLHPRCGTS
                     FLLIVMVVAIFVFAPLGLPAWYWLVISRIVGVPLIAGIAYELIKWAGRNRDKSWVRAV
                     MWPGLMLQNLTTREPDE"
     gene            complement(2843..3067)
                     /gene="rpmE"
                     /locus_tag="PLJ64_11535"
     CDS             complement(2843..3067)
                     /gene="rpmE"
                     /locus_tag="PLJ64_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HNC94156.1"
                     /translation="MKADIHPDYKLANVHCSCGNQFYTRSTKEDLHVELCSACHPFYT
                     GKQKLVDTGGRVERFQRRAAKRAKATAGQN"
BASE COUNT          535 a         1155 c         1088 g          553 t
ORIGIN      
        1 agccggcatc gcggaccatc ccggccacca gctccccctg cccctggccg atctcgaggc
       61 caatcaccgg ggccccgatt ccggcttccc ccagccccgc gagcacctcc gcgatcaccc
      121 gggttccgtc cggcccgccg aacagcgcac ccgcgggctc ccattgcccg acctcgggcg
      181 gcaggccatc cgagtcgggc acatacggga ggttggcgag gatcaggtca aacccggccg
      241 gaccttcagg caaagacccg agggtgaagc ggacccgctc ccccaggccg agccgtcccg
      301 cgtttgcgat cgccacctcg agtgccccgt cggagacatc ggtcgcggtc acctggcagg
      361 cagcaagttc gtcggcgacg gccagggcga tcgccccgga gccggtcccg atctcaagca
      421 ccgtgtccgg gttcacctcc cgggccagtt cgaccagcat ctcggtttcg ggccggggaa
      481 cgagaacccg gcgatcgacg gcgaggtcga tgtggcgaaa gcccttgcgg ccgaggatgt
      541 aggcgaccgg ctcgcgcctc agccgacgac ggaccgcttc cgaataggcc cgggcggccg
      601 ccggttccag gatcagttcg cggtcggcga tcagggcggc gcggtcgagt ccggccagct
      661 ccgccagcag gacctccgcg tccagccggg gcgtctcgat cccggccgcc gtgaaggcgt
      721 cgatcgccgc ggcgagggcc tcggcggcgc tcgccccgga ggcagccacg atcagaccgc
      781 ctgggcttcg aggcgctggc gcttttcctc ggcggccagc gcgtcggtga actggttgag
      841 gttcccgttc atgattccgt caaggtcgtg ggcggtcagc ttgatccggt ggtcggtcac
      901 ccggccctgg gggaagttgt aggtgcggat cttctcggag cgctccccgg ttccgacctg
      961 ggccttgcgt tcagcggcca ccgccgcctg ctgttcggcc agttgccgct cgtaaagacg
     1021 ggcccgcagc acccgcatcg ccttctccct gttttgaagc tgggacttct cgtcctgcat
     1081 cgacacgacg atgccggtcg gctgatgggt gatccggacg gctgaatcgg tggtgttgac
     1141 cgactgcccg cccggccccg acgagcggta gacgtcgatc tgaagatcgt tctggtcgat
     1201 gtccacttcg acctcctcgg cctccggcag cacggccacg gtcgcggtcg aggtgtggat
     1261 ccggccctgg gattcggtct ccggcacgcg ctggacccgg tgggtcccgc cctcgtactt
     1321 gtaaaccgag taggcgccgg cgccccggac ctcgaaggtc acgtccttga agccaccctg
     1381 ctcggccgcc gactgggaaa gcacctcggt cgagtagccg cgctcggcgg cgtagcgggt
     1441 gagcatcttg tagagatcac cggcaaacag ggcggcctcg tcgccaccgg tgccagcccg
     1501 gatttcgacg atcacgttct tgtcgtcgtt cggatccggc tcgaccatgg ccaggcggat
     1561 ctcctcctcg agctcttcca gccgggccgg ggcttcgcga accacctttt cgagatcctc
     1621 gtcctcgccc tcctcgagca gttcccgggc cccctcgaga tcgtcactca gggtccggta
     1681 ctcggtgacc agcgcattgg ccggctgaat ctggcggtac tcgcgaccga cctcggcata
     1741 ccgctcacgg tccccgatca cctcggggtc gctcatctgc tgctcaagct cggcaaagcg
     1801 agcctcaatc tgttccgcca nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 cttcgtccgg ctcgcgggtg gtcaggttct gcagcatcag gccgggccac atgaccgccc
     1981 gcacccagga cttatcccgg ttgcgcccgg cccacttgat cagctcgtag gcgatgccgg
     2041 cgatcagtgg caccccgacg atccgcgaaa tcaccagcca gtaccaggcg ggcaggccca
     2101 gcggagcgaa gacgaagatc gcgacgacca tgacgatcaa caggaagctg gtgccgcaac
     2161 gcggatggag ccgcgagtag agggcggccc gggaaggaat cagttcgtcc tcggcttcgt
     2221 agcaggagat cgtcttgtgc tcggccccgt ggtactcgaa gacccgccgc agatcctcca
     2281 gccgggagat catcacgatg taaccgatga agatcgcggt ccggacgatc ccttcgacca
     2341 gccagaagag aaaggaagag tcaagccagt ccttgatcag gctggtcaac ccgacgggaa
     2401 tcacgaagaa gagcaggatc gcgaaggcaa gcgagacgaa gatcgtcatc ccccaggcca
     2461 tgccgccaag ttcctcttcc tcctctccct cgtcgagctg ggcgttggcc gacatgccga
     2521 gggcccgaaa tccgatcttc agcgattcgg ccagggcgac gatcccgcgg atgatcggcc
     2581 agcgcaatac ccggtgccgg cgtgcccagg gttcgatctt ctcggtggtc gtctcgatct
     2641 cgccgtccgg cttgcggaca gagaccgccc atgtggtgac gccacgcatc atcacccctt
     2701 cgagcaccgc ctgaccccct accggggcgt cgcgcttggc ttcgagagga gcaggcgaaa
     2761 cggcgccaga ccgggtcggt gaggcgccgt tcgactgcat tcgctgttgg tctcgcgggc
     2821 cggcagccgg cccgggcgga acctagttct ggcctgcggt ggccttggcg cgcttggcag
     2881 cgcggcgctg gaagcgctcg acccgtccgc cggtgtcaac cagcttctgc ttgccggtgt
     2941 agaagggatg gcaggcagag cagagttcga catgcaggtc ttccttggtc gaccgcgtgt
     3001 agaactgatt gccgcacgaa caatggacgt tcgcgagctt gtaatcggga tggatatctg
     3061 ccttcatgaa acacattcta gagaagaggt caagccaccg acttctctgc tcgccgtggg
     3121 ggcacaagtg gtcacgccgt ggggccggtg gttacgccgt ggggccggtg gttacgccgt
     3181 ggggtcgggt gggggtagcc gggacggacg cacgcgcggc attcagccgc gctggtcctg
     3241 gagtgtcccg gacgacccca cccggccccg cggctacgcg gacccgggag aaacctcgac
     3301 gacccccacc cgaccccacg gctacacgaa ctcgggagat ccccacccga ccccgcggct
     3361 gcccgggccc tccgggggcg atttgcgaga gtccactttt ggccacatgg cgcactcaac
     3421 tccgatctcg a
//
LOCUS       DAOQKN010000226         3414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_17564_length_3414_cov_4.246204, whole genome shotgun sequence.
ACCESSION   DAOQKN010000226 DAOQKN010000000
VERSION     DAOQKN010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17564_length_3414_cov_4.246204"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2098
                     /locus_tag="PLJ64_11540"
     CDS             <1..2098
                     /locus_tag="PLJ64_11540"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC94157.1"
                     /translation="EASFNGAVEVVHVIWAAEDDGFAVVEVRDDSGEEFVATGTIGHL
                     KPGERAKLTGQWSEHQKYGPQVQVFTALPMDPSDRAGQVAYLSSLRHIGPIRAEALCE
                     LHGPEVLEKIAADPEGILGSLPGLGAKQREAALESWYETRAVRDLHVELAPHGLAHLA
                     GKIHARFGDKAMRTIREDPYSLTEIDGVGFARADTIAIAAGFPPESDRRAQAAAWYLL
                     GEAERRGHTHLPLNELVAQATKLLGYRPEPEVLTSASGVIAEEDRIYRQSTLDRECWL
                     AGDLRERAAGEAVLDHSPEPGADEKLTAEQWQAVAGAFGSRLSVVTGGPGVGKTVCTK
                     EIVREAKAAGVRIGLCAPTGRAARRLSEATGADAMTIHRLLEWRPGSEPTYKPGHPLP
                     VGMLIVDEASMINLHMADVLLNGLGHDTHIVFVGDADQLPPVGAGKPFADLIESGIVP
                     VTRLTHIFRQAARSMITTAAHEVNEGRPPHLTPGPDQVQDFHFMERVTPERVRSAVVE
                     MVGERVEAGLGLDPIRDAQVLAPIYRGEAGIDVLNRELQQRLNPEGRKAVSDRFRIGD
                     RLIQTRNAYEYGLMNGTICFLVDDDPDEEMVVLETDDGQSVELPYAETSDLKLAYAIS
                     VHKSQGCEIPVVIFVCHRSHSGMLTRPLIYTAITRAREMCVMVGDRPALENGVRRDEA
                     GRRYSSLAARLAGTLE"
     gene            2185..>3414
                     /locus_tag="PLJ64_11545"
     CDS             2185..>3414
                     /locus_tag="PLJ64_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94158.1"
                     /translation="MKPVGKLGALCFLLMLLFWGSESTASGLGRACTDECNAFYIAAS
                     ADGSEILFSSARPLVREDTNQGGDIYLWRDGTISLLSRTPEGLFREDDWKYPIWPDYR
                     SGSEARAVFSSANSYSPADTDKISDIYEVTPEGLTLLTHDSIFDRPCEKPCDLWSGNF
                     TEDGRTFFFDTKSPLLDEDTDSQRDAYRYENGEVTLVSGGPLAGGGDSRPVSSSDEGA
                     ASPDGSEFYFVTAESLDPADTDQATDVYVNRNGEVHLASPWAANYSVDLNPKASTEVP
                     GRFFMKTWAEFEGIAYRGMIYMAEGDNLYRLNLPGENTGGRQPEVEGESPDGETVFFS
                     TESSLDPRDRNDHTDLYAWRNGSVDLVNVGPRSSNSGYFGGSHLADADGTGGFAAKRP
                     GFAKPIHALSKVLNVVPP"
BASE COUNT          616 a         1178 c         1064 g          556 t
ORIGIN      
        1 cgaagcctcg ttcaacggcg cggtcgaggt tgtccatgtg atctgggcgg ccgaggatga
       61 cggcttcgcg gtggtcgaag ttcgcgacga ttccggtgag gaattcgtcg ccaccggaac
      121 gatcggccac ctgaaaccgg gtgagcgggc caaattgacc gggcaatgga gcgagcacca
      181 gaagtacggg ccccaggtcc aggtgttcac ggccctgccg atggatccct ccgaccgggc
      241 cggccaggtc gcatatctca gctcgctccg tcacatcggc ccgattcggg cggaggctct
      301 ctgtgaactc catggccccg aagtgctgga gaagatcgcc gccgaccccg aggggatcct
      361 cggttccttg cccgggctcg gggcaaagca gcgcgaggcg gcgctcgaga gctggtacga
      421 aacccgggcg gtgcgggacc tccacgtcga gctggcaccc cacggtctcg cccatctggc
      481 cggcaagatc catgcccgct tcggcgacaa ggcgatgcgg accatccgcg aagacccgta
      541 ttcgctgacc gagatcgacg gggtcggttt cgcccgggcc gacacgatcg cgattgccgc
      601 tggcttcccg ccggaatccg accgccgggc ccaggccgcc gcctggtacc tgctgggcga
      661 ggccgagcgc cgtggccaca cccacctgcc cctgaacgaa ctggtggccc aggccacaaa
      721 gctgctcggc taccgcccgg aaccggaagt tctgacctcg gcttcgggag tgatcgccga
      781 ggaggaccgg atctaccgcc agagcaccct cgaccgtgaa tgctggctgg ccggagacct
      841 cagggaacgc gctgccggtg aagcagtcct ggaccattca cccgaaccgg gcgcggacga
      901 gaaactcacc gccgagcagt ggcaggccgt ggccggcgcc ttcggctccc gcctttccgt
      961 ggtcaccggt ggccccggag tcggcaagac ggtctgcacg aaggagatcg tccgtgaggc
     1021 aaaggcggcc ggcgtgcgga tcggcctctg cgccccgacc gggcgggcag cccgccggct
     1081 ttccgaggca accggagccg acgcgatgac catccaccgc ctgctggagt ggcgtcccgg
     1141 ctcggagccg acctacaagc ccggccatcc cctgccggtc ggcatgctga tcgtcgatga
     1201 ggcctcgatg atcaacctcc acatggctga tgtgctgctc aacgggctag gccatgacac
     1261 tcacatcgtc ttcgtcggtg acgccgacca gctgccgccg gtcggggccg gcaagccctt
     1321 cgccgacctg atcgaatcgg gcatcgtccc ggtcacccgg ctgacccaca tcttccggca
     1381 ggcggcccgc tcgatgatca ccaccgccgc ccatgaggtg aacgagggcc gtccgcccca
     1441 cctcacgccg ggccccgacc aggtccagga cttccatttc atggagcggg tcacacccga
     1501 acgggtccgc agcgcggtcg tcgagatggt cggcgaacgg gtcgaggccg ggctcggcct
     1561 ggacccgatc cgcgacgccc aggtgcttgc cccgatctac cgcggcgagg cgggcatcga
     1621 cgtgctcaac cgcgaacttc agcagcggct caacccggag ggccgcaagg cggtcagcga
     1681 ccgcttccgg attggcgacc ggctgatcca gacccggaac gcctacgaat acggcctgat
     1741 gaacggcacg atctgcttcc tcgtcgatga cgacccggat gaggaaatgg tcgtgctcga
     1801 gaccgatgac ggccagtcgg tcgagctgcc ctacgcggag accagcgatc tcaagctcgc
     1861 ctacgcgatc tcggtccaca agtcacaggg ctgcgagatc ccggtcgtga tcttcgtctg
     1921 ccaccgctcc cactcgggca tgctcacccg ccccctaatc tacaccgcga tcacccgcgc
     1981 ccgggaaatg tgcgtgatgg tgggagaccg ccccgccctc gaaaacggcg tccgccgcga
     2041 cgaagccggc cgacgctact cctcgctggc tgctcgcctg gccggcaccc ttgagtagct
     2101 gccggcaggc gcaactctcc gcctgcatgg tgttctcatg aacactcagg cacttcatat
     2161 gcgccttatg tccaggaggg aaaattgaaa ccagtcggga aactcggggc tctctgcttt
     2221 ctgctcatgc tgctcttctg gggttcggaa tcgacggcga gcggccttgg ccgggcatgt
     2281 accgacgaat gcaacgcctt ttacatcgct gcctctgctg acggcagcga aattctcttc
     2341 agttcggctc gaccgctggt ccgcgaggac acgaaccagg gcggtgacat ctacctctgg
     2401 cgagacggga ccatctcgct tttgagcagg accccggagg gcctgttccg cgaagacgac
     2461 tggaagtacc cgatctggcc cgattaccga tccgggagcg aggcacgagc cgttttctcg
     2521 tcggccaact catattcccc cgccgacacg gacaagataa gtgacattta cgaggtcacc
     2581 cccgaaggtc tgacgctgct gacccacgac tcgatcttcg accggccatg tgaaaagccc
     2641 tgcgaccttt ggagtggcaa cttcaccgag gacggcagaa cgttcttctt cgacacgaag
     2701 tccccgctat tggacgaaga cacggactcc cagagggacg cgtaccgcta cgagaacggc
     2761 gaagtgacgt tggtttccgg aggcccactg gccggaggcg gcgattcccg cccggtctcc
     2821 tcttcggacg aaggggctgc gagccccgac gggtccgaat tctatttcgt gaccgccgaa
     2881 tctctcgacc ctgccgacac cgaccaggcc accgatgtct atgtgaaccg caacggagag
     2941 gtccacctcg cctcgccctg ggccgccaac tactcggtgg atctaaaccc caaggcgtcg
     3001 accgaagttc ccggccggtt cttcatgaag acctgggcgg aattcgaagg catcgcttac
     3061 cggggcatga tctacatggc cgaaggcgac aacctctacc ggctgaacct gcccggcgag
     3121 aacaccggcg gacgccagcc cgaggtcgaa ggtgaatccc cggatgggga aaccgtcttc
     3181 ttttcgaccg agtcgagcct cgacccccgg gaccggaacg accacaccga tctctacgcc
     3241 tggcggaatg gttcggtcga cctggtcaac gtcggccccc gctcctccaa ttccggctac
     3301 ttcggtggaa gtcacctggc cgacgccgat ggaaccggtg gctttgccgc caagcgaccg
     3361 ggctttgcca agccgatcca tgcattgtcc aaggtgctca acgtcgtgcc accg
//
LOCUS       DAOQKN010000227         3407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_17641_length_3407_cov_3.784010, whole genome shotgun sequence.
ACCESSION   DAOQKN010000227 DAOQKN010000000
VERSION     DAOQKN010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17641_length_3407_cov_3.784010"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="PLJ64_11550"
     CDS             complement(<1..359)
                     /locus_tag="PLJ64_11550"
                     /inference="COORDINATES: protein motif:HMM:NF024220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich protein"
                     /protein_id="HNC94159.1"
                     /translation="MATADTETIGYTGGPQAWTVPLGVHSVEFDLYGAAGSNNGNSGF
                     VGGKGGRAKATFDVSPGQLMRLYIGGTGGFLAGGWNGGGHGFAGGGGGTDLRIGGTGL
                     GNRVLVAGGGGGVGNYGG"
     gene            520..768
                     /locus_tag="PLJ64_11555"
     CDS             520..768
                     /locus_tag="PLJ64_11555"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HNC94160.1"
                     /translation="MATFSISKARESLSDAVERSRTEAVFLERHGKKAAVMVSPERYE
                     EMLEAFEEIEDVAAFDQAMAEESPNIPWDEAKADLGWD"
     gene            765..1025
                     /locus_tag="PLJ64_11560"
     CDS             765..1025
                     /locus_tag="PLJ64_11560"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNC94161.1"
                     /translation="MSRYRIELRPAALRSLRKLDPQIRRRVQGAIALLADDPRPPATR
                     KLKGRDAFRVRVDDYRVIYTIEDEVLLIVVVTLGHRRDVYER"
     gene            1276..2262
                     /locus_tag="PLJ64_11565"
     CDS             1276..2262
                     /locus_tag="PLJ64_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94162.1"
                     /translation="MSVKFRGFLGLLLAAVVLSTGHADAAKNGPILFSDYTSIWKIKA
                     DGSGLKRVAKKKAWSLHASPNGKQLLYTHDGLFRMPINGGRSVNLLKRYPIVDRFAGV
                     RWASWSPNGKQIAFEGGNDGRIYLIKSNGRGLRYLFRKYRTGLLHPVWSPNGKEIAYI
                     DVHNNSSLMAVNLKSGKKRTIYPGTGEAGTPVSFDWHPSGKRIAFYAPYRNWMIDSDG
                     SDLRQISPDQAFVSYEDLVFSPDGKSLLGRAYSGESSTSELWLLDGFYGAASGGFVNE
                     ITGRFGGSPFYPEWAPRSRVSRRARPRGLPGIRGGTFRRACRGFHRRRSATQ"
     gene            complement(2162..2584)
                     /locus_tag="PLJ64_11570"
     CDS             complement(2162..2584)
                     /locus_tag="PLJ64_11570"
                     /inference="COORDINATES: protein motif:HMM:NF021568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na-translocating system protein MpsC family
                     protein"
                     /protein_id="HNC94163.1"
                     /translation="MPSQSFGAARMTGADPGQATGHRKIAAEISDGMIELIREFYGTE
                     PSRTSTIYQDDLVLVVIVGGFTTIEKTLSEAGRTEEVSRLRHAFQDVMESRFKALVEN
                     ATGRKVTALLSGDDENPDMLGEMFLLESREGPEDEPAG"
     gene            2857..>3407
                     /locus_tag="PLJ64_11575"
     CDS             2857..>3407
                     /locus_tag="PLJ64_11575"
                     /inference="COORDINATES: protein motif:HMM:NF017758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HNC94164.1"
                     /translation="MSEAELFALRNEPRARDELVGRYRSLSKGIAFRYRSQAEIIDDL
                     IQVADLGLVAAVMKYAPRLARELDLAHAFEVHRHETAGHEQRVRERMDQLGHSPSRIK
                     DATGLAGGLGMLLFARSQPDTPGKLAAHAYSYEHMEIAAYGILAAYADRSGDRETAEL
                     ARRIESEERAMAGRISERFGEAVS"
BASE COUNT          634 a         1075 c         1079 g          619 t
ORIGIN      
        1 ccgccgtaat ttccgacacc gccaccgcca ccggccacaa gcactctgtt gcccagtccg
       61 gttccgccga tcctgaggtc tgtcccgccg cccccgccgg caaatccgtg accgcccccg
      121 ttccagccac cggcaagaaa gccgccggtt ccgccgatgt acagacgcat gagctggccc
      181 gggctgacgt cgaaggtcgc cttcgcgcga ccgcccttgc caccgacgaa gccgctgttg
      241 ccgttgttgc ttcccgccgc tccgtagaga tcgaactcga ccgagtgcac gccgagcggg
      301 acagtccagg cctgcgggcc acctgtgtag ccgatcgtct cggtgtcggc ggtggccaaa
      361 gtcggggacc cggcacacaa gagagcggcg atcaacccca caagtccgag ccgccatccc
      421 acccgcagta gaagtgcgct tctaaccggt cggctcatat ggagacaccc tatccggagt
      481 gttctgtaca aattggattc ggaactgtac aatttcagca tggccacttt cagcatcagc
      541 aaagcgaggg agtcactttc ggacgcggtc gagcggtccc ggaccgaagc cgtctttctc
      601 gagcggcatg gcaagaaggc tgcggtgatg gtcagtcccg agcgctacga agagatgttg
      661 gaagcgtttg aggagatcga agatgtcgct gcctttgatc aggcgatggc cgaagaaagt
      721 ccgaacatcc cctgggacga agccaaggcc gacctgggtt gggattgagc cgctatcgga
      781 tagagctgcg cccggccgcg ctccggtccc tgcgcaaact agacccccag atcagacgga
      841 gagtccaagg agcgatcgct ctccttgccg acgaccctcg cccgccagct accaggaaac
      901 tgaaaggacg agacgccttc cgggtccggg tggacgacta ccgggtgatc tacaccattg
      961 aagacgaggt gctgttgatc gttgtggtta cgctcgggca ccggcgggac gtctacgagc
     1021 ggtagatact gccgctgttg ccgtcgcctt agagggcgag ccaggggcgt gctgggccgg
     1081 acgcctcact ccgcgaagcg gttggtcttt tgtgcccgag cgggaaccac gcagggtgaa
     1141 agccgctggg atccggcctg ccgcgcctgg gcgatccata ccccgggctc gtactcgtaa
     1201 acgactgcca gggattcgcg tgcctatact tgcggcggtc cggggggtcc agtcgaacga
     1261 gcagggagaa gtgccatgag tgtgaagttc cgcggtttcc tcggattgct gcttgccgca
     1321 gtcgtccttt caaccggtca cgccgacgcg gcgaagaacg ggccgatcct gttcagcgac
     1381 tacacctcga tctggaagat caaggcagac ggttcgggcc tcaagcgggt ggcgaagaag
     1441 aaggcctgga gtcttcacgc ctctcccaac ggcaaacagc tcctctacac acatgacggc
     1501 ctcttccgga tgccgataaa cggcggcagg tcggtcaacc tgctcaagcg ctacccgatc
     1561 gtcgaccggt tcgccggtgt cagatgggct tcctggtcgc cgaacggaaa gcagatcgct
     1621 ttcgagggcg gcaacgacgg ccgcatctat ctgatcaagt cgaacggccg cggcctgagg
     1681 tacctgttcc gcaagtaccg gaccggcctg ctccaccccg tctggtcccc gaacgggaag
     1741 gaaatcgcct acatcgacgt ccacaacaac tcctcgctaa tggcggtgaa cctcaagtcg
     1801 ggtaagaagc gaaccatcta cccgggcacc ggtgaagccg ggacgccggt cagtttcgac
     1861 tggcacccga gcggcaagcg gatcgccttc tacgccccgt accggaactg gatgatcgac
     1921 agcgacggca gcgacctgcg gcagatctca cccgaccagg ccttcgtctc ctatgaggac
     1981 ctcgtcttct cgccggacgg gaagtcactt ctcggacggg catattcggg tgaaagctcg
     2041 accagcgaac tctggctgct tgacgggttc tacggcgccg cgagcggagg ttttgtgaac
     2101 gagatcaccg gccgcttcgg cggcagtccg ttctacccgg agtgggcccc gcgtagccgg
     2161 gttagccggc gggctcgtcc tcggggcctt cccgggattc gaggaggaac atttcgccga
     2221 gcatgtcggg gttttcatcg tcgccgctca gcaacgcagt gacttttctt cccgtggcat
     2281 tttcgaccag ggccttgaac ctcgactcca tgacgtcctg gaacgcgtga cgaagccggg
     2341 atacctcctc ggttcgaccc gcctcgctca gggtcttttc gatcgtcgtg aagcccccga
     2401 cgatcaccac cagcacaagg tcgtcctggt agatggtgct cgtccgtgaa ggctcggtgc
     2461 cgtagaactc cctgatcagt tcgatcatgc cgtccgagat ctcggcggcg atctttcgat
     2521 gtccggtggc ttggcctgga tcagcgccgg tcatccgggc ggccccgaaa gactgcgaag
     2581 gcaagcgatt gagccgggtt ggcgttccat gtgggagtcc ttctgtcggg ggtctggact
     2641 ggccggtacc cgggagcagg tgcgataacc actttgccga gggaggccgt ttgatacctt
     2701 cgtttcagaa gggcaaccgc cgacgccaac aggcagggct gcgacgtttg actttcgttc
     2761 gaatcagcta cacgaacgga aagtcttttg tcgcaagtac aaattcgtgg ttcatctcct
     2821 tcgaaagcca gtgcggcggg ccggctttac ggcctgatga gcgaagccga gttgttcgca
     2881 ctgagaaacg agccccgggc ccgggacgag ctggtcggtc gttacagatc cctgagcaag
     2941 gggatcgcgt ttcgctaccg gagccaggcc gaaattatcg acgacctcat tcaggtggcc
     3001 gacctgggcc tggtcgcggc ggtcatgaag tatgcgccgc gcctcgcccg cgagctcgat
     3061 ctcgcccacg cgttcgaggt tcaccgacac gagacggccg ggcacgagca gcgggtccgg
     3121 gagcggatgg atcagctggg gcactcgccg tcgcggatca aggatgcgac cggcctcgcc
     3181 ggtggcctgg ggatgctcct gtttgcccgc tcccagccgg acacacctgg caagctcgcg
     3241 gcccacgcct actcgtatga acacatggag atcgctgcgt acgggattct cgcggcctac
     3301 gccgatcgct ccggagaccg ggagaccgcc gagctcgccc gccggatcga atccgaagag
     3361 agggcgatgg ctggacggat atcggagcgt ttcggtgaag cggtatc
//
LOCUS       DAOQKN010000228         3404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_17661_length_3404_cov_4.765602, whole genome shotgun sequence.
ACCESSION   DAOQKN010000228 DAOQKN010000000
VERSION     DAOQKN010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17661_length_3404_cov_4.765602"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="PLJ64_11580"
     CDS             complement(<1..648)
                     /locus_tag="PLJ64_11580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC94165.1"
                     /translation="MESSKTNSLAVLIAARNEADVIAATIGSLREAFPGAVLWVADDA
                     SDDGTADAAMAAGAKVVSRGKPHGKGGNMTACAEAMLSDPGRDGEGPPDCVLLCDGDL
                     GASAGHLAPLVDAVEAGECDLAVAMFAKRVGGGFGFALGFSAWVIRRRCGAEPQAPIS
                     GQRAMKLDVLRRVLPFAPAYGMETGMTIDAVRAGYRLGEYEIDLAHKATGKSIKGF"
     gene            716..928
                     /locus_tag="PLJ64_11585"
     CDS             716..928
                     /locus_tag="PLJ64_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HNC94166.1"
                     /translation="MANIEAHITGNVWKIQVKVGDEVSEEDEVIILESMKMEIPVESE
                     DDGVVKEILCEEGQSVTEGDVLIVLE"
     gene            953..1774
                     /locus_tag="PLJ64_11590"
     CDS             953..1774
                     /locus_tag="PLJ64_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNC94167.1"
                     /translation="MADVTSIETGTEKVRLDFPAEAVARLKISNPPKRNALDHEILDA
                     ITATVPRLDQGIEVRCLVITGEDPVFSAGYDIGGIPAESFETDAEALVAHPFTAALDA
                     IEDFPWPTVAALNGHCLGGALEMAVTCDLRIAAPHAKLGMPPAKLGLIYGHTGLIRFI
                     RTIGLPRTRELFFTGDMLPAGRADEIGLVNRVTAGDEDFAVEAVEFAAGIAANAPLSM
                     KGNKQAIGTITENPVLSEDQVRELVELRESCFTSEDFLEGITAFGEKRKPNWTGR"
     gene            complement(1839..3041)
                     /locus_tag="PLJ64_11595"
     CDS             complement(1839..3041)
                     /locus_tag="PLJ64_11595"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNC94168.1"
                     /translation="MASALRKSPLRAAIGIALLLAGVAALLLLSSPGGDPGSSSGTTA
                     NSTGAGPFVKSESASSPFPPLTRNLSAGLTNHQMAGQRIVAGFEGTTVPPVIRSRIKA
                     GLIGGVILFADNLGSDARIRHLTRALQRIRRPDGLRRYPLMIMVDQEGGLVKRLPGAP
                     NASAEQMGRRGPAFSRRQGALAGLNLKTSGFNVDLAPVLDVARPGGVIADTDRGFGST
                     VKKVKATAIPFARALQQTRVAATAKHFPGLGAARQNTDFAVQKIRLTRAALRRVDMAP
                     YEPFIAARGKLIMVGSAIYPALGRRPAMFEPKIVRGELRQRLGFQGVTITDAMGTVAV
                     NDFGGTKRAAISAAHAGMDILLYGDWQSAGRAEQAIAVRLRTGKLPRGQFTRAVNRIL
                     QLRSSLAD"
     gene            complement(3104..>3404)
                     /gene="yaaA"
                     /locus_tag="PLJ64_11600"
     CDS             complement(3104..>3404)
                     /gene="yaaA"
                     /locus_tag="PLJ64_11600"
                     /inference="COORDINATES: protein motif:HMM:NF015820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HNC94169.1"
                     /translation="EGAGHDKEGNLVLDMRSGAYSAAWKPKQARLVTVRGFTETGGRR
                     KAISHMAKAVRGDVARIALSAKSMPRDPETLAGLLEAGGMTVELTEKTLDVIEQA"
BASE COUNT          571 a         1137 c         1152 g          544 t
ORIGIN      
        1 gaaccccttg atgctctttc cggtcgcctt gtgggcgagg tctatctcgt actcgccgag
       61 ccggtagccg gcccggaccg cgtcgatggt cattccggtc tccattccgt aggcgggagc
      121 gaaggggagt acccgtcgca gcacgtccag tttcatcgcc cgctgacccg agatcggcgc
      181 ctgtggttcg gccccgcagc gtcggcggat tacccaggcc gagaacccga gggcgaaccc
      241 gaagccgcca ccgacccttt tggcgaacat ggcgaccgcg aggtcgcatt cgcccgcctc
      301 gaccgcgtcg accagcgggg cgaggtggcc ggccgaagcg cccaggtccc catcgcagag
      361 caggacgcaa tccggcgggc cctcgccatc ccggccgggg tcgctgagca tcgcctcggc
      421 gcaggcggtc atgttgccgc ccttgccgtg gggcttgccg cggcttacga ctttcgctcc
      481 ggcggccatc gccgcgtcgg cggtgccgtc atcggaggcg tcgtcggcca cccagagcac
      541 ggcgccggga aaggcttcgc ggagagagcc gatcgtggcg gcgatcacgt cggcctcgtt
      601 tcgtgccgcg atcaggaccg ccagtgagtt ggtcttcgaa ctctccaccg tgggcggact
      661 ctagaggttg aggccgcgac ctctgtggat ggccgtggcg ataaagttcc ggctcatggc
      721 gaacatcgaa gctcacatca ccgggaacgt ctggaagatc caggtcaagg tcggggacga
      781 agtctccgaa gaagacgaag tgatcatcct cgagtcgatg aagatggaga tcccggtcga
      841 gtccgaggac gacggcgtgg tcaaggagat cctctgtgaa gagggccagt ccgtgaccga
      901 aggcgacgtc ctgatcgtcc tcgagtagcc gtcccccggc gagccgacgc cgatggctga
      961 cgtgacctcg atcgaaaccg gcacggagaa ggtccggctt gacttccccg cggaagcggt
     1021 cgcccgcctg aagatttcga acccgcccaa gcggaacgcg ctcgaccacg agattctcga
     1081 cgcgatcacg gccacggtgc cccggctcga tcaaggcatc gaggtccgtt gcctggtcat
     1141 caccggcgag gacccggtct tctcggcggg gtatgacatc ggcggcatcc cggccgagag
     1201 cttcgaaacc gacgccgagg ctctcgtcgc ccacccattc accgccgccc tcgacgcgat
     1261 cgaggacttt ccctggccga ccgtcgccgc cctgaacggt cactgcctcg gaggagcgct
     1321 ggagatggcg gtcacctgtg acctgaggat cgccgccccg cacgcgaagc tcggcatgcc
     1381 gccggccaag ctcggtctga tctacggcca caccggcctg atccgcttca tccgcaccat
     1441 cggcctgccc cgcacccgcg agcttttctt caccggtgac atgctgcccg ccggccgggc
     1501 cgacgaaatc ggtctggtca accgggttac cgccggggac gaggatttcg cggtcgaggc
     1561 ggtcgaattc gcggccggca tcgccgccaa cgcccccctt tcgatgaagg gcaacaagca
     1621 ggcgatcgga acgatcaccg aaaacccggt cctctccgaa gatcaggtga gagaactggt
     1681 cgaactccgc gagtcctgct ttacctccga agacttcctc gagggcatca ccgccttcgg
     1741 tgagaagcgc aagccgaact ggaccggccg ctagccgcga ccaggaccca cggttttcca
     1801 caatatttgt gggaaaccgt ggacttcgag acgaggcttc agtcagcgag gctggagcga
     1861 agctgaagga tccggtttac cgcgcgggtg aactggccgc ggggcagttt gccggtgcgc
     1921 aggcgaactg cgatcgcctg ttcggccctg ccggctgatt gccagtcgcc gtagaggaga
     1981 atgtccatgc cggcgtgggc ggcgctgatc gcggcacgct tggttccgcc gaagtcgttg
     2041 accgcgaccg tgcccatcgc atcggtgatc gtcacgccct gaaagccgag gcgctggcgg
     2101 agctcgccgc ggacgatctt cggttcgaac atcgccggcc ggcggcccag cgccgggtag
     2161 atcgcagagc caaccatgat cagcttgccc cgggcggcga tgaacggctc gtaaggggcc
     2221 atgtccaccc ggcgaagcgc cgcccgcgtg agccggatct tctggaccgc gaagtcggtg
     2281 ttctggcggg ccgcacccag acccgggaag tgcttggcgg tggcggcgac cctggtctgc
     2341 tgaagagccc gggcgaaggg gattgcggtc gccttcacct tcttcacggt tgagccgaaa
     2401 ccgcggtcgg tgtcggcgat gactcctccg ggacgggcca cgtccagcac cggagcgagg
     2461 tcgacgttga agcccgacgt cttgagattg aggccggcaa gagctccctg gcggcgggag
     2521 aaggcggggc cccgcctgcc catctgctcg gccgaggcgt tcggggcccc cggcagccgt
     2581 ttgacgagcc cgccttcctg gtcgaccatg atcatcagcg ggtagcggcg caggccgtcg
     2641 ggccgccgga tccgctgaag cgccctggtc agatgccgaa tccgcgcgtc cgagccgagg
     2701 ttgtcggcga agaggatcac tcccccgata agtccggcct tgattcggct gcggatgacc
     2761 ggagggacgg tggtgccctc gaacccggcg acgatccgct ggccggccat ctgatggttg
     2821 gtcagcccgg cggaaaggtt gcgggtcaag ggcgggaagg gcgagctggc gctctccgac
     2881 ttcacaaagg gcccggcccc ggtggaattc gccgtggtgc ccgaagaaga gcccgggtca
     2941 ccgcccggcg aggaaagcag caggagagcc gccacaccgg ccagcaaaag cgcgatgccg
     3001 atcgccgcac gcaggggtga ttttctcagg gcggaagcca tacatcgatg atacggatcg
     3061 gccccgaacg gcccgcctgc caaagcagaa gagcgggtcc ggatcaggcc tgttcgatga
     3121 cgtcgagggt cttttcggtc aactcgaccg tcattccccc ggcttcaagc aggccagcca
     3181 gggtttcggg atccctcggc atggacttcg ccgagagggc gatgcgggcg acatcgccgc
     3241 ggaccgcctt ggccatgtgg gagatcgcct tgcgcctgcc cccggtctcg gtgaagcccc
     3301 ggaccgtaac cagccgggcc tgcttcggct tccaggcggc cgagtacgcc ccggagcgca
     3361 tgtcaaggac gaggtttcct tccttgtcgt ggccggcccc ctcc
//
LOCUS       DAOQKN010000229         3314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_18483_length_3314_cov_5.379871, whole genome shotgun sequence.
ACCESSION   DAOQKN010000229 DAOQKN010000000
VERSION     DAOQKN010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18483_length_3314_cov_5.379871"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2331
                     /locus_tag="PLJ64_11605"
     CDS             <1..2331
                     /locus_tag="PLJ64_11605"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC94170.1"
                     /translation="MKGFKSFPEKTELAFSPGVSVIVGPNGSGKSNITDAVLWCLGEQ
                     SPGAIRGTSMQDVISVGGKNVGARSEAEVEVVIDNAEGQLGDEFTEISITRKLDRNGD
                     GGYWMNGARCRLSDIIELLSDANLGREMHSVISQGRVDSIVHSKPRERRLLIEEAAGL
                     GKFRKRRRRSELKLLATRDNLDRALDVEREARKALRPLKQQANSAEIGARIERQELEL
                     KSRLVAEDLRFGEARLEQARTAAEKARKVRDGLEGELTAVSRRRRAAEERFAERDRER
                     TEIWGILNRLRSGQEKVSIESSRIAEQGRRLQLRLEDLRLELAPLTLDVGESGSASER
                     ARRLVEELSEVDAGIATAAEGLRQARAEGPEAKRLKALGDVHKGAERATTHARRAESL
                     LGERHRERLGERMAEVEKLGDALTSLTASADQTGQAIRDRVARAERTVIGDQGDADEI
                     SAELKQCSDLEVEIQGKLRAVAEQVTGAEVEAAHLGDRKAEAAGELARISGILETEIP
                     EPEEPLEDEEREQIEKRLVNLRTRRERLGPVNPLAKKEYEEALEHVEQLVAQREDTER
                     AMRELEGVIRDIDAEIKRSFDETFAATAKNFEEMIAQLFPGGSGRLRSVDLRPVPQPA
                     AEGSDAEGEEEAEEIEDEDGPRTPSESDFGVELEVTPQGKRMRQMSLLSGGEKAMTAL
                     AFVFAVFLARPCPFYILDEVEAALDDANINRFLELVRRFSDRTQFVIVTHQKLTMDAA
                     DVLYGVSMGGDGVTKVISRRLPKGVTALEGTQLEAA"
     gene            2377..>3314
                     /gene="ftsY"
                     /locus_tag="PLJ64_11610"
     CDS             2377..>3314
                     /gene="ftsY"
                     /locus_tag="PLJ64_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNC94171.1"
                     /translation="MSTTWNDILDLGDRAVPDTAPPPAAEEEAPKKMGAFRRLRESLS
                     KSRRALTEEIGSSLFDRIDDETWERLEEALILADAGATTTAQVVEKLEYEVDSGAVPP
                     DGAAIKERLIGILTEIASTNRAPINLSQDPAVVLMTGVNGTGKTTSIGKIAWHLRNEF
                     GLSVLLGAADTYRAAAAEQLATWAERAGADLVRSNEGADPGSVAFDAINAARSRGSDV
                     VIIDTAGRLHTQSNLMDELGKVKRVIDKQIPGAPHETLISIDATTGQNGLRQAKAFAE
                     ATEVSGVVLTKLDGTAKGGITLAIATELGIPVKLIGV"
BASE COUNT          647 a         1102 c         1124 g          441 t
ORIGIN      
        1 atgaagggct tcaagtcctt cccggaaaag accgagctgg ccttctcgcc cggggtcagc
       61 gtgatcgtcg ggccgaacgg ctccgggaag tcgaacatca ccgacgccgt gctctggtgt
      121 ctcggcgagc agagcccggg cgcgattcgc ggcacctcga tgcaggacgt gatttcggtc
      181 ggcggcaaga acgtcggcgc ccgctccgag gccgaggtcg aggtcgtgat cgacaacgcc
      241 gaaggtcaac tgggggacga gttcacggaa atctcgatca cccgcaagct ggatcgaaac
      301 ggagacggcg gctactggat gaacggcgcc cgctgccggc tgtcggacat catcgaactt
      361 ctctccgacg ccaacctcgg gcgggagatg cattcggtga tcagccaggg ccgggttgat
      421 tcgatcgtcc actccaagcc ccgcgagcga cgcctgctga tcgaagaggc cgccggactc
      481 ggcaagttcc gcaagcgccg gcgccgctcg gagctgaagc tgctcgccac ccgcgacaac
      541 ctcgaccgcg ctctcgacgt cgagcgcgag gcccgcaagg cgctcaggcc gctcaagcag
      601 caggcgaact cggccgagat cggggcccgg atcgaacgcc aggaactcga actgaagagc
      661 cggctggtcg ccgaggatct ccgcttcggc gaagcccgcc tggaacaggc ccggacggcc
      721 gccgaaaagg cacgcaaggt ccgcgacggg ctcgaaggcg aactgaccgc ggtttccagg
      781 cgccgccgcg ccgccgagga acgctttgcc gaaagggacc gcgagcgaac cgagatctgg
      841 ggcatcctca accggctgcg ttccggccag gaaaaagtgt caatcgagtc gtcgcggatt
      901 gccgagcagg gccggcgact tcagctccgc ctggaagacc tccggctcga gctggctccg
      961 ctgaccctcg acgtgggtga gagcggcagc gccagcgagc gggccaggcg gctggtcgaa
     1021 gagctctccg aagtcgacgc cggaattgcc acggcggccg aggggctgcg gcaggcccgg
     1081 gccgaggggc cggaagccaa gcggctgaag gcactggggg acgtccacaa gggcgccgaa
     1141 agggcgacca cccacgcccg ccgggccgag agcctgctcg gtgaaaggca tcgagagcgg
     1201 ctgggtgagc gcatggccga ggtcgagaag ctgggcgatg ccctgacttc actaactgcc
     1261 tcggccgatc agactggtca ggcgattcgg gaccgggtcg cccgggcgga gcggaccgta
     1321 atcggggacc agggcgacgc cgacgagatc tcggccgaac tcaagcagtg ctcggacctg
     1381 gaagtcgaaa tccaggggaa gctgcgggcc gtggccgagc aggtgaccgg ggcggaagtg
     1441 gaagccgccc atctcggcga tcgcaaggca gaagcggccg gtgagctcgc ccgcatttcg
     1501 ggaatcctcg aaaccgagat ccccgagccc gaggaaccgc tcgaagatga agagcgcgaa
     1561 cagatcgaaa agcgcctggt gaacctgcgg acccgccggg agcgcctcgg gccggtcaat
     1621 ccgctggcca agaaggaata cgaggaagcg ctggagcatg tcgagcaact cgtcgcccag
     1681 cgggaagaca ccgaaagggc gatgcgggaa ctggaagggg tgatccgtga catcgacgcc
     1741 gagatcaagc gctccttcga tgagactttc gccgccaccg cgaagaactt cgaagaaatg
     1801 atcgcccagc ttttccccgg tggctccggc cggctccgtt cggttgacct gcgccccgta
     1861 ccccagcctg ccgccgaagg gtcggacgca gaaggggaag aggaagcgga ggagatcgag
     1921 gacgaggacg gcccccgcac cccgagcgaa tccgacttcg gggtcgaact cgaagtcaca
     1981 ccgcagggca agcgcatgcg ccagatgagc ctgctttccg gcggcgagaa ggcgatgacc
     2041 gcgctggcct tcgtcttcgc ggtcttcctc gcccggccct gcccgttcta catcctcgac
     2101 gaggtcgagg ccgccctcga tgacgccaac atcaaccgtt tcctcgaact ggtccgccgc
     2161 ttctcggatc gcacacagtt cgtgatcgtg actcaccaga agctgaccat ggacgcggcc
     2221 gacgtgctct acggggtcag catgggtggc gacggcgtga ccaaggtgat ctcccgtcgc
     2281 ctgccgaagg gggttaccgc cctcgaaggc acgcagctgg aagccgcgta accgcttctc
     2341 tgacaccggc aacccgattc cggcaagatc cgaaccgtga gcacgacctg gaacgacatc
     2401 ctcgacctcg gtgaccgcgc cgtccccgac accgccccgc cacccgccgc cgaggaggaa
     2461 gcgccgaaga agatgggcgc gttccgacgg cttcgcgaaa gcctctcgaa gagccgccgg
     2521 gcgctgaccg aggaaatcgg ttccagcctc ttcgaccgga tcgacgacga gacctgggag
     2581 cgcctcgagg aagcgctgat cctggccgac gcgggagcga ccacgaccgc ccaggtggtc
     2641 gagaagctcg aatacgaggt cgattccgga gcggtgccgc cggatggcgc ggcgatcaag
     2701 gagcgcctga tcggcatcct gaccgagatc gcctcgacca accgggcgcc gatcaacctc
     2761 agccaggatc ccgccgtggt cctgatgacc ggagtcaacg gcaccggcaa gaccacctcg
     2821 atcggcaaga tcgcctggca cctcaggaac gagttcgggc tgtccgtcct gcttggcgcc
     2881 gccgacacct accgggcggc cgcggccgag cagcttgcga cctgggccga gcgggccggg
     2941 gcggacctgg ttcgctcgaa cgaaggcgcc gaccccggct cggttgcctt cgacgcgatc
     3001 aatgccgcca ggtcccgcgg ctccgatgtt gtgatcatcg atacggccgg ccggcttcac
     3061 acccagtcga acctgatgga cgagctgggc aaggtcaaac gggtcatcga caagcagatc
     3121 cccggtgccc cgcacgaaac cctgatctcg atcgacgcga ccaccggtca gaacggtctg
     3181 cgccaggcca aggccttcgc cgaagcgacc gaggtctccg gagtcgtcct gaccaagctc
     3241 gacggaaccg ccaagggcgg gatcaccctc gcgatcgcga ccgagctcgg catcccggtc
     3301 aagctgatcg gagt
//
LOCUS       DAOQKN010000230         3314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_18484_length_3314_cov_4.601105, whole genome shotgun sequence.
ACCESSION   DAOQKN010000230 DAOQKN010000000
VERSION     DAOQKN010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18484_length_3314_cov_4.601105"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1077)
                     /locus_tag="PLJ64_11615"
     CDS             complement(<1..1077)
                     /locus_tag="PLJ64_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNC94172.1"
                     /translation="MARPISAPVVRRSFSRLETPLEAPHLIDIQRRSFEDLTDPEHGM
                     LRETINDVSPIEDYTGNLAIVFGEFEFDDPAHTLEECRERDLTYSRPLNVTVSFQNRE
                     TGEIREQTVFMGDFPWMTERGTFIINGTERVVVTQLVRSPGAYVMAPKDPEKQVFTAN
                     LMPARGSWLELEIDKKGRVYVRIDRKRKLPITVLLRSMGYVSNEQILDLFDNSVYIQH
                     TIAADTEATLTEEGALIELFKKQRPGEPPSVDAAKNLLEQLFFDPKRYDLTRVGRYKL
                     NARLGLDIDLDTRVLTHDDIISLIKELVSLPKLLGMPEVIDEENPIKDYAAEALTYPR
                     ENVSDHLDEYEHFGNRRLRTVGELI"
     gene            complement(1329..1649)
                     /locus_tag="PLJ64_11620"
     CDS             complement(1329..1649)
                     /locus_tag="PLJ64_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94173.1"
                     /translation="MPELNRIKSVYEGWYLLVWIERGHPMPLVNERVFGREIDLFFPG
                     KKFGLELDGGQFHSDPAQKEVDREKMLFLESRGLTMERLPYKVFAADPIGEVDRIARQ
                     LGFC"
     gene            complement(2604..2993)
                     /gene="rplL"
                     /locus_tag="PLJ64_11625"
     CDS             complement(2604..2993)
                     /gene="rplL"
                     /locus_tag="PLJ64_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNC94174.1"
                     /translation="MATSTEEWIEELKGISVLELSERIKALEEEFGVSATAVAAAAPA
                     AGAAGGDAGDGADEGSSTVDVVLTDVGDKKVPVIKVVRAATGLGLKEAKALVDEAPKP
                     VKEGVEKDEAEKLKAELEEAGASVELK"
     gene            complement(3000..>3314)
                     /locus_tag="PLJ64_11630"
     CDS             complement(3000..>3314)
                     /locus_tag="PLJ64_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94175.1"
                     /translation="ASPLVTLTRGLGSMISGLAIALQQVADQGLVGGNSEAAPAEEAA
                     VEEKVEEAPAEEVTEEAPAEKADASDEETEEAPAAEATDGADENDEAPAEEATDGADA
                     KE"
BASE COUNT          603 a         1032 c         1018 g          661 t
ORIGIN      
        1 gatcagctca cccaccgtgc gcaggcggcg gttgccgaag tgctcgtact cgtcgaggtg
       61 gtcggagacg ttctcgcgcg gataggtcag ggcctcggcc gcgtagtcct tgatcgggtt
      121 ctcttcgtcg atcacttccg gcatgccgag cagcttcggc aggctgacca gctccttgat
      181 cagggagatg atgtcgtcgt gcgtgagcac gcgggtgtcg aggtcgatgt cgagaccgag
      241 acgggcgttc agcttgtagc ggccgacccg ggtgaggtcg tagcgcttcg ggtcgaagaa
      301 gagctgctcg agcaggttct tggccgcgtc aaccgacggc ggctcgcccg ggcgctgctt
      361 cttgaacagc tcgatcaggg cgccttcctc ggtcagcgtg gcctcggtgt cggcggcgat
      421 cgtgtgctgg atgtagaccg agttgtcgaa caggtcgagg atctgctcgt tgctcacgta
      481 acccatcgaa cgaagcagca cggtgatcgg cagcttgcgc ttgcggtcga tgcggacgta
      541 gacgcggccc ttcttgtcga tctcgagctc gagccaggaa ccacgggccg gcatcaggtt
      601 cgcggtgaag acctgcttct ccggatcctt cggcgccatc acgtaggcgc cgggggaacg
      661 gaccagctgg gtcacgacga cacgctcggt gccgttgatg atgaaggttc cacgctcggt
      721 catccacggg aagtccccca tgaagacggt ctgctcgcgg atctcgccgg tctcgcggtt
      781 ctggaaggaa accgtgacat tcagcggacg cgagtaagtg agatcgcgct cgcgacactc
      841 ttcgagagtg tgagcggggt cgtcaaattc gaattcaccg aagacgatgg cgagattgcc
      901 ggtgtaatcc tcgatgggtg aaacgtcgtt gatcgtctcc ctcagcatgc cgtgctcggg
      961 atcggtgaga tcctcaaacg agcgacgctg aatgtcgatc agatgtgggg cttcgagggg
     1021 ggtttcgagt cgggagaaac tcctgcggac aacgggggca gaaatcgggc gtgccaaggt
     1081 tggcaactcc tcacaagggg tcagagattc acgtgattag ggcgcggaag cggtgcaaat
     1141 cggccgaaac ttcgcgcaac gacaaacaat agcacaggaa gaacggctcc taacacagtg
     1201 cccggacgca atcccggccc cggcccaccg agattgaaat caaggacaaa tagcgctctt
     1261 catttcaatc tcgccgcccc cgctcccatc ttcctcgcct gacaacgcga aactgccgga
     1321 ccgtcagctc agcaaaaacc tagctgccgg gcgatccggt cgacctcccc gatcggatcg
     1381 gcagcaaaga ccttgtaggg cagccgctcc atggtcaggc ctcgggattc gaggaacagc
     1441 attttctcgc ggtcaacctc cttttgggcc ggatcgctat gaaattggcc gccatccagt
     1501 tctagtccga atttcttccc agggaagaac aggtcaattt cccggccaaa tactctttca
     1561 ttgaccagcg gcatcgggtg tccccgttca atccagacca gcaggtacca gccttcatag
     1621 accgacttga tccgattcag ttcaggcacc cacaggccca gatagggtcg aagcttcctt
     1681 gcgccacgcc gaccggcgag ccgttcgtag ctgtacctaa ggtccggtct gccgaacaac
     1741 cgaagatgac aggcttcgag gaaagcgaat cgaacctcgg ggtcggaggc atcggcggag
     1801 aaatcaacga gagcatgagc aggcctggcc agccttattc ctcctttggt ccggacatcc
     1861 tccggcctca gatcccttcg gaccactctg atcgtcgtgt ctcgcagagc aggcgaacag
     1921 ccctttagtt ccctgacctg gccgaaggga ctgccgacct gcactagccc cgcgagtccg
     1981 ggcctctccc tgacaatccc ccaagcctcg caggcactcc gccccgctat tgccgaaccc
     2041 gggccggcca cgagcaaagc aacccgacgg agggcttccg aagtctggac atcacgtccg
     2101 tacgagtaga ccgagtgggc gaccctgacc agcctcccgc ttcgcagcca gttcttgatc
     2161 tgggaggcac tgaagccagc ggctaccagt tcgcccaggc tggcgacaaa ttcccgggcc
     2221 gccatgaagc catacagccg acgcagttgc cccttcggag ttgcctttat cgagagagaa
     2281 gtatgcaccc ctcaagcctt gcggagaaat gctcacaagt ggctctacag gtgtgacgat
     2341 tctgcaacgc aggggctgaa cggaggagag ctgactgcgc ttccctctca ccagcccttc
     2401 caccctggcg ggcgagaagg tggcccagat tcagtgaggt tgaaatggag agcgctattt
     2461 gtccttgaat tcaacctcgg tgttctgggg cggggcgttc gggcggggcg tgatttcggg
     2521 gcgtgatttc gggttaacga cgaagggcgc ctttcggcgc ccttcgtgaa gcgtcggggt
     2581 ctcccccggc tctggaagaa ctcttacttg agttcgaccg aagcgccggc ttcttcgagt
     2641 tcggccttga gcttttcggc ctcgtccttc tcgacgcctt ccttgacggg cttgggggcc
     2701 tcgtcgacca gagccttggc ctccttgaga ccgagtccgg tggcggcgcg aacgaccttg
     2761 atgaccggga ccttcttgtc gccgacgtcg gtcaggacga cgtcgacagt cgagctgccc
     2821 tcgtcggcgc cgtcaccggc gtctccaccg gcggcgccgg cggccggagc tgcagcggcg
     2881 acggccgtgg ccgaaacgcc gaactcctct tcgagagcct tgatgcgctc gctcagctcg
     2941 aggacgctga tgcccttgag ctcttcaatc cattcttcag tgctggttgc catttctggt
     3001 tactccttag cgtcggcacc atcagtggct tcttcagccg gcgcctcgtc gttttcatcg
     3061 gcaccatcag tggcctctgc ggccggcgcc tcttcggttt cttcatctga agcgtcggct
     3121 ttttcggccg gcgcctcttc agtgacttcc tcggcgggag cctcttcaac cttctcctcg
     3181 acggctgctt cttcggccgg agcggcctcg gagtttccac cgaccagacc ctgatcggcc
     3241 acctgctgga gggcgatcgc gaggccggag atcatcgatc ccaggccgcg ggtcagggtg
     3301 accagcgggc tggc
//
LOCUS       DAOQKN010000231         3275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_18888_length_3275_cov_4.818323, whole genome shotgun sequence.
ACCESSION   DAOQKN010000231 DAOQKN010000000
VERSION     DAOQKN010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18888_length_3275_cov_4.818323"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="PLJ64_11635"
     CDS             complement(<1..593)
                     /locus_tag="PLJ64_11635"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HNC94176.1"
                     /translation="MPEDAQGNAASVRNRDQLGAQGIGELRLKALQIAEAGLAACDGG
                     AATRKRVRLTPDGVMIDGVLHRLDADQRLLVLGGGKATLPVAQALEEVLGDRIDGGAI
                     VLKRGQEAELEGLEVMTSDHPFPSPDSLAAAGKIAGLAAEACEGDLVITCFTGGRSAL
                     LTLPPETVTFDEKRRLHELLIASGMPITAINTVRKHVS"
     gene            complement(627..1604)
                     /locus_tag="PLJ64_11640"
     CDS             complement(627..1604)
                     /locus_tag="PLJ64_11640"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HNC94177.1"
                     /translation="MPTADSGFLNRIRSFSISPETYQVVTLIALVVLVIIVFTGAAVR
                     LTASGLGCPDWPKCYGGVVAPPQINAWIEYGNRLVTGVVSITVIAAALLAFLRKPYRW
                     HLAFFGILLPLGVIGQAILGAFVVYYHLPPELVIFHFILSMILIDAAFALFWCSRYEP
                     GERRYSTDWVSTWAVRALIPIGQLTIFLGTITTASGPHPGDHDEELVRRFDFKGTDTL
                     EWVVQRHAAIAVIFGLSVLAVIFIIRRQGGDRRAQKPLIVTLGLICLQLAVGITQWLL
                     HLPAGLVWVHVVVATLLWLAVLWSVAAAGQITPGTKGALIRKPEAAPTG"
     gene            complement(1591..2307)
                     /locus_tag="PLJ64_11645"
     CDS             complement(1591..2307)
                     /locus_tag="PLJ64_11645"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNC94178.1"
                     /translation="MKMNKFSTKLPVLLASVAILGAVIAGCGTSSSNSDPEELGFQDR
                     GRQLFNANCGTCHVLAQAASTGTQGPNLDDAFAAARLAGVDDDTIKGIVRAQVHRPQV
                     VNGNYPGVTMPANLLEGNDLEDVAAYVAAVAGVPGIEPPKAPGEGAGAQIYANNGCGS
                     CHTLAAAQSAGTLGPNLDEVLPKMSKAEIHEAIVNPGAKVENGYPNAMPSDYASKISE
                     EQLNQLIEFLMTSAAADANG"
     gene            2452..2871
                     /locus_tag="PLJ64_11650"
     CDS             2452..2871
                     /locus_tag="PLJ64_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNC94179.1"
                     /translation="MRVSVGMSEVVLRVGPDHTLREAARKMADRSVGAAIVEHDEWPG
                     PQILTERDVLRAVGEGLDPDTEKVGDHMSEKVITASPEWSLERAATEMSKRGIRHLTV
                     YRDGELIGVLSMRDIMRVWTSDGATSSQAGATTSHPV"
BASE COUNT          589 a         1012 c         1135 g          539 t
ORIGIN      
        1 gacacatgct tgcggacggt gttgattgcg gtgatcggca tgcccgatgc gatcagcagc
       61 tcgtgaaggc gcctcttctc gtcaaaggtg actgtttcgg gaggcagcgt aagcagcgcc
      121 gaacgaccgc cggtgaagca ggtgatcacc aggtcgcctt cgcaggcctc cgcggccagc
      181 ccggcgatct tgccggcggc ggcaagcgag tctggcgagg gaaagggatg gtcggacgtc
      241 atcacctcca gcccttcgag ttctgcttcc tgaccgcgct tcaggacgat ggcaccaccg
      301 tcgatccggt ctccgagcac ttcctccagg gcttgggcaa ccgggagggt cgccttgccg
      361 cccccgagga ctaaaagtcg ctggtcggcg tcaagccggt gaaggacacc gtcgatcatc
      421 acgccgtccg gagtgagcct cacccttttg cgggttgcgg cgccgccgtc acaggcggcg
      481 agtccggctt cggcgatctg caacgccttg aggcggagct cgccgattcc ctgtgcgccg
      541 agctgatccc gattgcgaac cgaagcggcg tttccctgcg cgtcttccgg catcagcgag
      601 caatcactgg gaggtcagcc ggggtttcag ccggtgggtg ccgcttcggg cttgcggatc
      661 agtgccccct tggtgccggg ggtgatctga ccggccgcgg ccaccgacca gaggaccgcc
      721 agccagagca gggtcgcgac cacgacatgg acccagacca ggcccgcggg caggtggagc
      781 agccactggg tgatgccgac cgccagctgg aggcagatca ggcccagggt cacgatcagc
      841 ggcttctggg cgcgacggtc gcctccctga cgccggatga tgaagatcac agcgaggacc
      901 gagaggccga agatcaccgc aatcgccgcg tgtcgctgca cgacccactc gagtgtgtcg
      961 gtgcccttga agtcaaagcg ccggaccagc tcctcgtcgt ggtctcccgg atggggaccg
     1021 gaggcggtcg tgatcgtgcc gaggaagatg gtgagctggc cgatcgggat cagcgccctt
     1081 accgcccagg tgctgaccca gtcggtcgag tagcgccgtt caccgggctc gtaacgggaa
     1141 caccagaaga gcgcgaaggc ggcgtcgatc aggatcatcg agaggatgaa gtggaatatg
     1201 accagctccg gggggaggtg gtagtagacg acgaacgcgc cgaggatggc ctggccgatc
     1261 actcccagcg gcaggaggat cccgaagaag gcgaggtgcc agcgataggg cttgcgcaga
     1321 aaggccagca gcgcggcggc gatgacggtg atcgagacga cgccggtgac caggcggttg
     1381 ccgtattcga tccaggcgtt gatctggggc ggcgcgacca ccccgccgta gcacttgggc
     1441 cagtccgggc agccaagccc ggaagcggtc aggcgcaccg ccgccccggt gaagacgatg
     1501 atgaccagga cgaccagcgc gatcagggtt acgacctgat aggtctccgg cgagatcgaa
     1561 aaactgcgga tccggttcag gaagccggaa tcagccgttg gcatcagccg cagctgatgt
     1621 catcaggaac tcgatcagct ggttcagctg ctcttcgctg atctttgatg cgtagtcgga
     1681 aggcatcgcg ttcgggtagc cgttttcaac tttcgccccc gggttcacga tcgcctcatg
     1741 gatctccgcc ttggacatct tcgggagaac ctcgtcgagg ttcgggccga gggtgccggc
     1801 ggactgggcg gcggccaggg tgtggcagct gccgcagccg ttgttggcgt agatctgggc
     1861 accggcgcct tcgcccggag ccttgggcgg ctcgatgccg ggcactccgg cgaccgcggc
     1921 cacataggcg gcgacgtctt cgaggtcgtt gccttcaagc aggttggcgg gcatggtcac
     1981 gccgggatag ttgccgttga cgacctgggg gcggtggacc tgggcgcgaa cgatgccctt
     2041 gatcgtgtcg tcgtccacac cggcgaggcg ggccgcggcg aaggcgtcat cgaggttcgg
     2101 gccctgggtt ccggtgctgg cggcctgggc gagaacgtgg caggtgccgc agttggcgtt
     2161 gaagagctgg cggccgcgat cctggaatcc gagctcttcc ggatcggagt tcgaagacga
     2221 agtgccacaa cccgcgatca ccgcgccgag gatggcgacg gaggcgagga ggacgggaag
     2281 cttggtcgag aacttgttca tcttcatggc gatcggtgaa gcccggccat tctatatatc
     2341 ggggcggcat cgccgccttg acgtaagcga aggggagcat ctcccatttg agacaactcg
     2401 cacaatccgc taggctggca actatcgtca caaactaagg aaagggatcg gatgcgcgta
     2461 agtgtcggaa tgtcggaagt agtgctccgg gtgggcccgg accacacact gcgtgaggcc
     2521 gcccgcaaaa tggccgaccg ctcggtcggc gctgcgatcg tcgaacacga tgaatggccc
     2581 ggtccccaga tcctgaccga gcgggacgtg ttgcgcgcgg tcggcgaggg ccttgacccc
     2641 gacaccgaaa aggttggcga ccacatgagc gagaaggtga tcaccgcttc gcccgagtgg
     2701 agcctcgaac gcgccgccac ggaaatgtca aagcgcggca tccgccacct cacggtctac
     2761 cgcgacggtg aactcatcgg cgtgctcagc atgagggaca tcatgcgtgt ctggacctcc
     2821 gacggagcca cctcaagcca ggccggcgcc acgaccagcc accccgtctg acccgacccg
     2881 cacccgcaca cggcggccgg ctaggcccat ttcgcgagat tccgtttctc gccacataac
     2941 gcacccaact ccgatctcga cctgaactac cccgattctg aggcggtttc gactccctcg
     3001 tcacctcaat gccctgttcc cgcagtgggt cggatttccg tcgcagcagc tggaaatccg
     3061 acccaccgga ccgtgcgaaa agggccgcgg ggccggtcgg ctgctcccgg gatccaggtg
     3121 ggtcggattt ccggggcggt gacggaaaac cgacccaccg agcgtgaggg tggggctgga
     3181 atcgtcgagt gccctgtttg tgccggtatg cggcacaaag tgggatgtcg cggggtcggg
     3241 ggtcgccgat cgaccccgga tgggtcggac cgact
//
LOCUS       DAOQKN010000232         3250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_19145_length_3250_cov_5.659468, whole genome shotgun sequence.
ACCESSION   DAOQKN010000232 DAOQKN010000000
VERSION     DAOQKN010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19145_length_3250_cov_5.659468"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(234..776)
                     /locus_tag="PLJ64_11655"
     CDS             complement(234..776)
                     /locus_tag="PLJ64_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HNC94180.1"
                     /translation="MSEENNDFTTDQAIREESGSHPYGRFLEEFEVGATYKHMPAKTV
                     TEYDDHLFCLLTMNHHPLHINDVHANASQQGKNVVVGPLVYSLAVGMSVSDFSGKAIA
                     NLATDELSHPAPVFHGDTLFCESEVLEVRESKSKLDRGLVKVHTRVFNQDGTLVCHFK
                     RTALIPRKVHGVPTETNDVD"
     gene            complement(821..1186)
                     /locus_tag="PLJ64_11660"
     CDS             complement(821..1186)
                     /locus_tag="PLJ64_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94181.1"
                     /translation="MASTAQMIESAVTHLQEEVPALKKLKLVFGLDLRAKGDMQTYRV
                     ELPGPKVSKGRGEDERCRVEIDRQQFNQLADEKSTVGSYRRAAETGHIRFEGDSNVAK
                     LVIRVIEAHEQRAKLKKVH"
     gene            complement(1221..1661)
                     /locus_tag="PLJ64_11665"
     CDS             complement(1221..1661)
                     /locus_tag="PLJ64_11665"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNC94182.1"
                     /translation="MAVETIDEYLDSLPEDRREAIEAVRSAVAAGIPEGYAEELRWGM
                     ISWEIPLETYPETYNGQPLAYVSLASQKNHMALYLMGIYSDEGNRAWFENALKERGTK
                     LDIGKSCVRFKRLGDLPLDLVTEAVGRFSVNDYIQTYEKSRGLA"
     gene            complement(1689..2888)
                     /locus_tag="PLJ64_11670"
     CDS             complement(1689..2888)
                     /locus_tag="PLJ64_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNC94183.1"
                     /translation="MSSYQEITEDQKAIIEMVHQFVDEQIIPNAEHYDHEDSFPEPIV
                     EQMKELGLFGVTIAEEYGGMGLDLSTYELIVEELSRGWISISGVVNTHFIGVYLLQQF
                     GSEEQKQKYLPKMATGEMRAAFSLSEPEVGSDVQGIKTGAKKDGDAYVIDGQKMWVTN
                     GLGSSLVFLLAKTDPGAEKAHQGMTCFITEKVPWVEENTGEYAGLNVPPKIKKMGYKG
                     VESTELVYNGYRCPAENILGGEEAGLNKGFPQMMDALEVGRVNVAARGVGIAQRALEL
                     ALQYSQERKTFGKPIANHQAIQFKLADMATKLEAARLVTRKAANMKDAGIRSDLEAGM
                     AKLIASETGKECVEECFRIHGGYGYSKEYEIERLYRDAPLLLIGEGTSEIQRMVIGKK
                     LLQRHKI"
     gene            complement(3026..>3250)
                     /locus_tag="PLJ64_11675"
     CDS             complement(3026..>3250)
                     /locus_tag="PLJ64_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94184.1"
                     /translation="LRAAWHGVSRAKSLAVIAPRTLRAGKSARIRVVLPARLARKLNR
                     SVSPGRLAVTVGARTARGRATLVRRSLAVG"
BASE COUNT          506 a          978 c         1075 g          691 t
ORIGIN      
        1 ccggaagttg cccttgcaga ctctcagcgg gtggatgcca ggggcctcca gccgttcgct
       61 gccgaggaga caggccggaa atggccgcga tcgtacgtgg gtacttgagc tgccgtttcc
      121 ggccgtcgac gaagccatcc ggccgttcag cgagaccgtc attgaacgac catcgagcga
      181 acgagccgga tgttccttgt gttgcggctg gtggttcctg gcgccgcccg ggctcagtcg
      241 acgtcgttgg tctcggtggg aacgccgtga accttgcggg ggatcagggc ggtccgcttg
      301 aagtggcaga cgagggtgcc gtcctggttg aagacgcggg tgtggacctt gaccaggccg
      361 cggtcgagct tgctcttgga ctcgcggacc tcgaggacct cggactcgca gaagagcgtg
      421 tcaccgtgga aaacgggggc cgggtggctg agttcgtcgg tggcgaggtt ggcgatcgcc
      481 ttgccggaga agtcggaaac cgacatgccg actgccagcg agtagaccag cgggccgacc
      541 acgacgttct tgccctgctg cgaggcgttc gcatgaacgt cattgatgtg cagcgggtgg
      601 tggttcatgg tcagcaggca gaagaggtgg tcgtcgtatt cggtgaccgt cttggccggc
      661 atgtgcttgt aggtggcgcc gacctcgaac tcctcgagaa agcggccgta ggggtgcgag
      721 ccggactctt cgcggattgc ctggtcggtg gtgaagtcgt tgttttcttc gctcattgtc
      781 tggttggttt tccttggttg gtgacttaag aagtcagtgt tcagtggact ttcttcagct
      841 tcgcccgctg ttcatgcgcc tcgattacgc ggatgaccag cttcgccaca ttcgaatctc
      901 cctcgaaccg gatgtgacct gtttcggcgg cacgccggta ggaaccgacc gtgctttttt
      961 cgtcggccag ctgattgaac tgctggcggt cgatctcgac ccggcagcgt tcgtcctctc
     1021 ctcttccctt gctcaccttc ggcccgggca actcgacccg gtacgtctgc atgtcaccct
     1081 ttgcccggag atcgagaccg aagaccagct tcagcttctt caaggccggc acctcctcct
     1141 ggaggtgggt gacggcggat tcgatcatct gcgctgtgga tgccatgcgt ggtgtttatc
     1201 agggaaaccg cgtgattgcc tcaggcgagg ccgcggctct tctcgtaagt ctggatgtag
     1261 tcgttgacgc tgaaccggcc gaccgcttcg gtgacgaggt ccagcggcag gtccccgagc
     1321 cgcttgaacc ggacgcagga cttgccgata tccagcttgg tcccgcgctc cttgagggcg
     1381 ttctcgaacc aggcccggtt gccttcgtcc gagtagatgc ccatcaggta gagggccatg
     1441 tggtttttct gcgaggcgag cgacacgtag gcgagtggct ggccgttgta ggtctccgga
     1501 taggtctcga gcgggatctc ccagctgatc attccccagc ggagttcttc cgcgtagccc
     1561 tcgggaattc cggccgctac ggccgaccgg accgcttcga tcgcttcccg gcggtcttcg
     1621 ggaagggaat cgagatattc gtcaattgtt tcgacggcca tcccgatccc ttccctcagc
     1681 ccgtcagatc agatcttgtg gcgctggagc agcttcttgc cgatcaccat gcgctggatt
     1741 tccgacgtgc cctcgccgat cagaagcagc ggggcgtccc ggtagaggcg ctcgatctcg
     1801 tattccttcg agtagccgta gccgccgtgg atgcggaagc attcctcgac gcattccttg
     1861 ccggtttcgg aggcgatcag cttggccatt ccggcttcga ggtcggaacg gatgccggcg
     1921 tccttcatgt tggccgcctt gcgggtgacc aggcgggccg cttccagctt ggtcgccatg
     1981 tcggccagct tgaactggat cgcctggtgg ttggcgatcg gcttgccgaa ggtcttgcgc
     2041 tcctggctgt actgaagggc gagctcgagg gcgcgctggg cgatgccgac gccacgggcg
     2101 gcgacgttga cgcggccgac ctcgagggcg tccatcatct gcgggaaacc cttgttcagg
     2161 ccggcttcct cgccgccgag gatgttctcg gccgggcagc ggtagccgtt gtaaaccagc
     2221 tcggttgact cgacgccctt gtagcccatc ttcttgatct tgggcggaac gttgaggccg
     2281 gcgtactcgc cggtgttctc ctcgacccac ggcaccttct cggtgatgaa gcaggtcatg
     2341 ccctgatggg ccttctccgc ccccgggtcg gtcttggcga gcaggaagac gaggctcgag
     2401 ccgagtccgt tggtgaccca catcttctgg ccgtcgatca cgtaggcgtc gccgtccttc
     2461 ttggcgcctg tcttgatgcc ctggacatcg gagccgacct cgggctcgga gagcgagaag
     2521 gcggcacgca tctcgccggt ggccatcttc ggcagatact tctgcttctg ctcttcgcta
     2581 ccgaactgct ggagcaggta gacgccgatg aagtgggtat ttaccacacc ggagatcgag
     2641 atccagccgc gggaaagctc ctcgacgatc agttcgtagg tcgagaggtc gagccccatc
     2701 ccgccgtatt cctcggcgat ggtcactccg aagaggccca gttccttcat ctgctcgacg
     2761 atcggctccg ggaaggaatc ctcgtggtcg tagtgctcgg cgttggggat gatctgctcg
     2821 tcgacgaact gatggaccat ctcgatgatg gccttctggt cctcggtgat ctcctgatag
     2881 ctggacatgg ctcctcttgg ggtttggtgg cgcagactga gttgaagcgc gctgtggcgc
     2941 ggaaaagacc cgcgaaaagc acgggcaaaa aggggcaaca aggatatcgg gattggcctt
     3001 ctgacgcggt ttccgggagc cggccctaac cgaccgcgag gctgcggcgg accagcgttg
     3061 cgcgacccct ggcggtgcgc gcaccaaccg ttacggccaa gcgaccgggc gagaccgacc
     3121 ggttcagttt gcgggccagg cgagcgggca gcaccaccct gatccgggcg ctcttgccgg
     3181 ccctcagcgt gcgcggcgcg atgaccgcca gcgacttcgc acgggagact ccgtgccagg
     3241 ctgccctgag
//
LOCUS       DAOQKN010000233         3250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_19150_length_3250_cov_4.590923, whole genome shotgun sequence.
ACCESSION   DAOQKN010000233 DAOQKN010000000
VERSION     DAOQKN010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19150_length_3250_cov_4.590923"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /locus_tag="PLJ64_11680"
     CDS             complement(<1..988)
                     /locus_tag="PLJ64_11680"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HNC94185.1"
                     /translation="MEPTSAPSKPKPADKPKPKPGDKGIEQEEGKLQEGKGRLGTLTN
                     PETYENVPGHIIPIIEEEFVDFEGEANEFLDGNTPEDQFIGFRLKQGVYGQRQPGVQM
                     VRVKLPFGGVTPEQMDAFADAVETYAPLNKGHITTRQNIQIHHIPLNQMVHMLRDLAP
                     SGLSSREGCGNTVRNVTGDPWAGVAEDEIFDPTRYAGAYTRYFVRHPTTQLMPRKIKT
                     SFTGSDADRAMSGIHDIAFLSRIRDGVKGFEIRVGGGTSIMPRVAPTIYDFVEAENGD
                     YLKHAEAILRVYDRQAWLRPNRARARIKVLVDKVGIEEVRRMVDEELKGDWVN"
     gene            complement(1252..1656)
                     /locus_tag="PLJ64_11685"
     CDS             complement(1252..1656)
                     /locus_tag="PLJ64_11685"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNC94186.1"
                     /translation="MSDEEATEVQPYERSSAEVAELIAAGARLIDVRTAHEHEAARLA
                     GSERIGLEELLDRRDEIEQDGTVIFYCRVGNRSGMAAQAFSEAGFDVVNLTGGIVQWI
                     EDGQPIEPADGYVAEPGEAAAILEARARAAGN"
     gene            complement(1791..2000)
                     /locus_tag="PLJ64_11690"
     CDS             complement(1791..2000)
                     /locus_tag="PLJ64_11690"
                     /inference="COORDINATES: protein motif:HMM:NF018961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin domain-containing protein"
                     /protein_id="HNC94187.1"
                     /translation="MAVVKIIELVGSSRVSTDDAAQQALKQAQDSLRNIRAVDIVSTG
                     IRGENLDEYRAHVRVAFLIEGKDVT"
     gene            complement(2079..2282)
                     /locus_tag="PLJ64_11695"
     CDS             complement(2079..2282)
                     /locus_tag="PLJ64_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94188.1"
                     /translation="MSEITISQSTTPQEAAAIAAAIQRFSQDTAIAPATEPAGMNPWL
                     KAALEDGVSAKDAFGPGQPFGTL"
     gene            2367..>3250
                     /locus_tag="PLJ64_11700"
     CDS             2367..>3250
                     /locus_tag="PLJ64_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94189.1"
                     /translation="MKTARAARILFGVALLASAALLLHYRLDIGFVADDWAFLRDRSL
                     GGLDSLLIPENENIVILQAAVYRIFYILFGIQSSASLQIASLVFFLLSVVAFYAWIRE
                     RVGEWAAAIASIPILFLGTAYQDLLWAFQMGYTVSILAGLTALIALERNARFSQPVAL
                     VAILVSVTTSSLGLPFLAGAAVILVSRDGWEGLFNYVLLAPFLVFLVWWVGWGRTADS
                     HLSLTNLLHAPAFVAESVKSVVMGLTGLHRTDSVAGDVLAWVVSGALFAAVSFSLIRR
                     KRLPLPLLVALAVGLAFWV"
BASE COUNT          468 a         1023 c         1086 g          673 t
ORIGIN      
        1 cgttcaccca gtcgcccttc agttcctcgt cgaccatgcg gcggacctct tcgatgccga
       61 ccttgtcgac caggaccttg atccgggcgc gggcccggtt cgggcgcagc caggcctggc
      121 ggtcgtaaac ccgcaggatc gcttcggcgt gcttgaggta gtcgccattt tcggcctcga
      181 cgaagtcgta gatcgtgggg gcgacgcggg gcatgatcga ggtgccgccg ccgacccgga
      241 tctcgaagcc cttgacgccg tccctgatcc ggctgaggaa ggcgatgtca tggatgccgc
      301 tcatcgcccg gtcggcgtcg gaaccggtga aggaagtctt gatcttccgc ggcatcagct
      361 gggtcgtggg gtgccggacg aagtagcggg tgtaggcgcc tgcgtacctg gtcgggtcga
      421 agatctcgtc ttcggcgacg ccggcccagg ggtcgccggt cacgttgcgg accgtgttgc
      481 cgcagccctc gcgggacgag aggccgctgg gggcgaggtc gcgcagcatg tggaccatct
      541 ggttcagcgg gatgtggtgg atctggatgt tctgccgggt cgtgatgtga cccttgttga
      601 gcggcgcgta agtctcgacc gcgtcggcga aggcatccat ctgctccggg gtgacgccgc
      661 cgaaggggag cttcacgcga accatctgca cgccgggctg gcgctggccg tagacgccct
      721 gcttgaggcg gaacccgatg aactggtcct cgggggtgtt gccgtccagg aactcgttcg
      781 cctcaccctc gaaatcgacg aactcctctt cgatgatcgg gatgatgtga ccggggacgt
      841 tctcgtaggt ctccgggttg gtcagcgttc ccagccggcc cttgccttcc tggagcttgc
      901 cttcctcctg ctcgatcccc ttgtcaccgg gcttcggctt gggcttgtcg gcgggcttcg
      961 gtttggacgg ggccgaagtg ggctccaatt gggtttcctc ctgctgcgaa aaaggcttgg
     1021 ttgctgacgt gggctgacaa cggtagcaaa aagaccaaaa cctgaccgga tttcatgtaa
     1081 ttagaagtcg ggccggtccg accgaccctc gagtggctac ccgtacctgt ctatggaccg
     1141 gttctgcagg gttcgggtcg gcagtgcttc cagaaaacct ccggaatgag gagcgcagcg
     1201 acggttttcg ggaagtactg ccgttccggg ccccgtcgtc aagccgctcg gctagttgcc
     1261 tgctgctctg gcgcgggctt cgaggatggc ggcggcttcg cccggttcgg ccacgtagcc
     1321 gtcggccggc tcgatcggct ggccgtcctc gatccactgg acgatgccgc cggtcaggtt
     1381 caccacgtca aagcctgctt cggagaatgc ctgggccgcc atgccggaac ggttaccgac
     1441 ccggcagtag aagatgaccg ttccatcctg ctcgatctca tcgcggcggt cgagcagctc
     1501 ctcgagcccg atccgctcgg aaccggccag gcgcgcagcc tcgtgctcat gcgcggtccg
     1561 cacatcgatc aaacgcgcac cagccgcaat cagttccgcc acctcggcag aactccgctc
     1621 gtaaggctgg acttcggttg cttcttcgtc actcatgggt ccggaaaggt agcagggagg
     1681 acaggcctgg cccaccccag gcagagcccc tcccaaccat caattacttc aagtgggagg
     1741 ggcgggtgtc aactggaggg cgggtcggac cccgcccgac tcctgttctc ctaagttacg
     1801 tccttgcctt cgatcaggaa cgcgacgcgg acgtgggcgc ggtactcgtc gaggttttcc
     1861 ccgcggatgc cggtcgagac gatgtccaca gcgcggatgt tgcgaagcga atcctgggcc
     1921 tgcttgagcg cctgctgggc tgcgtcatcg gttgaaaccc gtgagctgcc aaccagctcg
     1981 atgatcttca ctaccgccat gaactcctcc tcggtcgatt tgactcgatg ctagttgctc
     2041 gcgcgaagaa gtgcgagggg gcggagcagc gggactcgtc agagcgtgcc gaagggctgg
     2101 ccgggaccga aagcatcctt ggccgagacc ccgtcttcga gggcggcctt cagccaggga
     2161 ttcatcccgg ccggttcggt cgcgggggcg atcgcggtgt cctgcgaaaa gcgctgaatc
     2221 gccgcggcga tcgcggcggc ttcctgcggg gtcgtggact ggctgatcgt gatttcgctc
     2281 atgcggcgtc agattctatg ctcgcccgct cccgcgacgg ccgccgatag gctgcgccca
     2341 tcgtgggagc cggtttcagt gagggcatga agactgcgcg agccgcccgg attctcttcg
     2401 gggtggcgct tctggcatcg gccgccttgc tgcttcacta ccggctcgac atcggtttcg
     2461 tggccgacga ctgggccttt ctgagggatc gcagcctggg cgggctcgat tcgctgctga
     2521 tcccggagaa cgagaacatc gtcattctgc aggcggcggt ctaccggatc ttctacatcc
     2581 tgttcggaat ccagtcctcg gcgtcgcttc agatcgccag tctcgtcttc ttcctgctga
     2641 gcgtggttgc cttctacgcg tggattcgcg agcgggtggg cgagtgggcg gcggcaatcg
     2701 catctatccc gatcctcttc ctcggcaccg cctaccagga cctgctctgg gccttccaga
     2761 tggggtacac ggtttcgatc ctggccgggc tgacggccct gatcgccctg gagcgaaacg
     2821 caaggttcag ccagccggtg gctctcgtcg ccattctggt ctcggtcacg acctcgtcgc
     2881 tggggctccc cttcctcgcc ggtgccgcgg tcatcctcgt ctcgagggac ggctgggagg
     2941 gcctcttcaa ctacgtgctt ctggcgccgt ttctggtgtt tctcgtttgg tgggtcggct
     3001 ggggaaggac ggctgacagt cacctgagcc tgaccaatct cctccacgcc ccggccttcg
     3061 tggcggagtc ggtcaagtcg gtggtgatgg gattgaccgg actccaccgc accgattcag
     3121 tggccgggga cgtgctggcc tgggtcgttt cgggggcact cttcgccgcg gtctcgttca
     3181 gcctgattcg ccgaaaacgg ctgccgctgc cgttgctcgt cgcgctggcg gtcgggctcg
     3241 ccttctgggt
//
LOCUS       DAOQKN010000234         3228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_19405_length_3228_cov_6.056098, whole genome shotgun sequence.
ACCESSION   DAOQKN010000234 DAOQKN010000000
VERSION     DAOQKN010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19405_length_3228_cov_6.056098"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..1310)
                     /locus_tag="PLJ64_11705"
     CDS             complement(90..1310)
                     /locus_tag="PLJ64_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HNC94190.1"
                     /translation="MSATAPVRLSSAAARRIALAAQGFGRERPFPRVTMRQVQAMIDR
                     IGLLQIDSVNVLARAHLLPLYSRLGPYDIDLLEKASSKRPRRLVEYWAHEASFVPPET
                     HRLLRWRMDRADQDAWRTVRAAAEQPELLEDVVAEVERSGPVTAGQVAAAVDPDHVPD
                     KSNWGWNWPPVKSALEYLFWSGRITAAGRTSQFERRYDLPGRVLPGHVHEAETPEPEE
                     AIEGLLEISARAHGVGTARCLRDYFRLPARESKEAIERLAADGRLIEAEVEGFSAPAF
                     LHPEARRPRKVEARALLAPFDPLIFERRRLEELFGFHYRIEIYTPKEKRRHGYYVLPF
                     LMAEQIAGRVDLKSDREGSALLVQSAWVEPDAPAGTAAELADELRLMAGWLGLEEVAV
                     RPRGDLAADLAAAL"
     gene            1340..2764
                     /locus_tag="PLJ64_11710"
     CDS             1340..2764
                     /locus_tag="PLJ64_11710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HNC94191.1"
                     /translation="MSVHSHIITANATSEGEDLMSGDARAHAYRNVIITAATAGLAGL
                     LFGYDTGVIAGALLQITPDFGLGSFESGLVVGAVPLGAVLGAWIASSRSDKYGRRTLI
                     LTSGIIFIIGAIVAAISPGTWVLVASRVVIGVAIGVASAVAPVYISEIAPPDIRGRLV
                     TFFQLAVTIGILVAYLVGLAFADSNEGWRWMLGLGAVPALALVIGIIRLPSSPRWLLM
                     VGREDDAREALQRVRTEGPEAIELEIKEIESSMSIHEGSWRDLLAPAVKAALVVGIGL
                     AILQQITGINTVIYYAPTIIQETGIDSDASSILASLGVGIVNVLMTVVALRLLDTRGR
                     REMLFVGVSGISISLFILGLAFLIGADSTFATVMAIGSLMLFVSSFAISLGPIFWLLN
                     AEIYPLSVRSKAASAGTMTNWFFNFLVSLTFLPLIDLLSQTGAFWFYGAIGLVTLWFC
                     WKYVPETKGRSLEQINDIFERRAR"
     gene            2886..>3228
                     /locus_tag="PLJ64_11715"
     CDS             2886..>3228
                     /locus_tag="PLJ64_11715"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC94192.1"
                     /translation="MSQTPSCSIRDFRIEDAEAVHRWFNNKEATKTLMEQRESFGMEN
                     AEGWTRAAMKQEGEDRKYAIEVPGIEQPVGFTALYGLFRQTAPELGCLIGDEVRGKGV
                     GRWAERLTVTKA"
BASE COUNT          492 a         1114 c         1056 g          566 t
ORIGIN      
        1 gagatggagc cgcccggtcg ggctcggggc gtagcggccc ggggcagctt ccggctcccg
       61 gtcgggatga aacaggtcga gcctggcggt cacagggccg ccgccagatc cgcggcgaga
      121 tctcccctgg gccggaccgc cacctcttcg aggccgagcc acccggccat cagcctgagt
      181 tcgtcggcca gttccgccgc ggtcccggcc ggggcatccg gctcgaccca ggccgactgc
      241 accagcagcg ccgatccctc ccggtcggat ttgaggtcca cccggccggc gatttgttct
      301 gccatcagaa acggcagcac gtagtagccg tggcgacgct tctccttcgg ggtgtagatc
      361 tcgatccggt agtggaagcc gaaaagctcc tcgagtcgac gcctctcgaa gatcagcgga
      421 tcgaatggag cgagcagcgc ccgcgcctcg accttgcggg gtcgacgggc ctcggggtgc
      481 aggaaggccg gagccgagaa cccttcgacc tcggcctcga tcaggcgccc gtcggcggcg
      541 agccgttcga tcgcctcctt cgactcccgg gccggcaggc ggaagtagtc cctgaggcag
      601 cgcgccgtgc cgaccccgtg ggcccgggcg gagatctcca gcaatccctc gatcgcttcc
      661 tccggctcgg gagtttccgc ttcgtggacg tggccgggca gcacccgccc cggcaggtcg
      721 tagcgtcgtt cgaactggct ggttcgcccg gccgcggtga tccggccgga ccagaacagg
      781 tactcgaggg cgctcttgac cggcggccag ttccatcccc agttgctctt gtcggggaca
      841 tggtccgggt cgacggcggc cgcgacctga ccggcggtca ccggacccga tcgctccacc
      901 tcggcgacta cgtcctcgag cagttccggc tgttcggcgg cggcccgtac cgtgcgccag
      961 gcgtcctggt ctgcccggtc catccgccag cggagcagcc ggtgggtttc cggtggaacg
     1021 aaggaggcct cgtgggccca gtactcgacc agccggcgcg gccgcttcga actcgccttc
     1081 tcgagcagat cgatgtcata gggcccaagc cgggagtaga gcggcagcag gtgtgccctg
     1141 gccagcacgt tgaccgagtc gatctggagc aggccgatcc ggtcgatcat cgcctggacc
     1201 tgacgcatcg tcaccctcgg aaaggggcgt tcgcgaccga agccctgcgc ggccagggca
     1261 atccggcggg ctgccgccga actcagcctc accggggccg tggcactcat ccgaccaagc
     1321 ctcgctccca acttgcgtca tgtccgttca ctcgcacata attaccgcca acgccacttc
     1381 cgagggggag gacctcatga gcggagatgc ccgcgcgcac gcatatcgca acgtaatcat
     1441 cacggccgcc accgccgggc tggccggact tctgtttggt tacgacaccg gtgtgatcgc
     1501 cggggccctg cttcagatca cccccgactt cggtctcggc agctttgaat ccggtctggt
     1561 cgtcggcgcg gtgccgctgg gggccgtgct cggcgcctgg atcgccagct cccgctctga
     1621 caagtacggc cgccgcaccc tgatcctcac ctcggggatc atcttcatca tcggcgcgat
     1681 cgtcgcggcg atttcacccg gtacctgggt tctggtcgct tcccgtgtgg tgatcggcgt
     1741 cgcgatcggc gtcgcctccg ccgtggcgcc ggtctacatc agcgagatcg ccccgcctga
     1801 catccggggc aggctcgtca cgttcttcca gctggcggtg acgatcggca tcctcgtcgc
     1861 ctacctggtc ggtctcgcct ttgccgattc gaacgaaggc tggcgatgga tgctcgggct
     1921 cggcgccgtg ccggcgctgg cgctcgtgat cgggatcatc cggctgccct ccagcccgcg
     1981 ctggctgctc atggtcggcc gcgaggacga tgcgcgcgag gcgctgcagc gggtcaggac
     2041 cgaaggcccc gaagcgatcg agctggagat caaggagatc gagtccagca tgagcatcca
     2101 cgagggctcc tggcgggatc tgctcgcccc tgccgtgaaa gcggcgctgg tggtcggcat
     2161 cgggctggcg atcctccagc agatcaccgg gatcaacacg gtcatctact acgccccgac
     2221 gatcatccag gagaccggga tcgactccga cgcctcctcg atcctcgcct cgctcggggt
     2281 cggcatcgtc aacgtgctga tgaccgtggt cgcgctgcga ttgctggaca cccgcggccg
     2341 ccgcgagatg ctcttcgtcg gcgtgtcggg gatctcgatt tcgctgttca tcctcggcct
     2401 cgccttcctg atcggcgccg attcgacctt cgccacggtg atggcgatcg gcagcctgat
     2461 gctcttcgtc tcctcgtttg cgatctcgct cggcccgatc ttctggctgc tgaacgcgga
     2521 gatctacccg ctctcggtca ggagcaaggc ggcctcggcc ggcaccatga ccaactggtt
     2581 cttcaacttc ctggtctcgc tgaccttcct gccgctgatc gacctgcttt cacaaaccgg
     2641 cgccttctgg ttctacggcg cgatcggcct ggtcaccctc tggttctgct ggaagtacgt
     2701 cccggagacc aagggacgca gcctcgagca gatcaacgac atcttcgaac gccgagcccg
     2761 ataacccggg ctccagcgtt cgcaccggga gtcgggcggg ttcggacccg ccctcccgct
     2821 gaaacccgcc cctcccactt gaagcaacga atggtctgga ggggcttcgc ctttaggatt
     2881 acggcatgtc gcagaccccc tcctgctcga ttcgggactt ccggatcgag gacgctgaag
     2941 cggttcaccg ctggttcaac aacaaggaag caaccaaaac gctgatggaa cagcgtgagt
     3001 ccttcggcat ggagaacgcc gaagggtgga cccgggcggc gatgaagcag gagggcgagg
     3061 accgcaagta cgcgatcgag gtgccgggga tcgagcagcc ggtcggcttc accgcgcttt
     3121 acggactctt ccgccagacc gcacccgaac tcggttgcct gatcggtgac gaggtgcggg
     3181 gcaagggggt cggccgctgg gccgaacggc tgaccgtgac caaggctt
//
LOCUS       DAOQKN010000235         3197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_19750_length_3197_cov_5.097708, whole genome shotgun sequence.
ACCESSION   DAOQKN010000235 DAOQKN010000000
VERSION     DAOQKN010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19750_length_3197_cov_5.097708"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="PLJ64_11720"
     CDS             complement(<1..224)
                     /locus_tag="PLJ64_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94193.1"
                     /translation="MNSRGLAFLAALFVVVAAFALPSLASADPDITPPPTPVDPDGPL
                     LVSSGGSATFTFDDTIGGDVDHYVCKVTKSP"
     gene            complement(355..771)
                     /locus_tag="PLJ64_11725"
     CDS             complement(355..771)
                     /locus_tag="PLJ64_11725"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HNC94194.1"
                     /translation="MSVLTPEATRLIESDALAHLVTLNRDGSPQLSCVWVGLDGDEIV
                     SGHMMKRLKLKNVERDPRIVLSIESPSANEMGLKEYLVVHGSARVEEGGAAALLQRLA
                     HIYMGPDAVFPPPGIDAPGWVLRITPERLGGVGPWA"
     gene            870..1331
                     /locus_tag="PLJ64_11730"
     CDS             870..1331
                     /locus_tag="PLJ64_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNC94195.1"
                     /translation="MPSTVEYENLLRGAELRVTQPRMAVLSAVHDNPHADTTTIIDAA
                     RIQVPEVSHQAVYDVLRALTGAGLIQHIQPPGSVARYESRVGDNHHHVVCRSCGAIAD
                     VDCAVGSAPCLTASEDHGFVIEEAEVVYRGLCPDCAAKSTNEQSPRKESRV"
     gene            1324..>3197
                     /gene="katG"
                     /locus_tag="PLJ64_11735"
     CDS             1324..>3197
                     /gene="katG"
                     /locus_tag="PLJ64_11735"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HNC94196.1"
                     /translation="MSEQNDQPDAVIGEVNEESSGGCPVVHDRFPYPTESGGNIDWWP
                     NRLNLKILAKNPVETNPYGDDFDYRAEFESLDLGAVKKDINEVLTTSQDWWPADFNHY
                     GPLIIRMAWHSAGTYRVMDGRGGGGHGQQRFAPLNSWPDNVNLDKARRLLWPVKQKYG
                     RKLSWGDLMILAGNVALESMGFKTFGFAGGRVDEWEPDEDVYWGPEKEWLGDERYTGE
                     RDLDNPLAAVQMGLIYVNPEGPNGNADPVAAAKDIRETFARMAMNDEETVALIAGGHT
                     FGKTHGAADPEPYIGPEPEAAPLEEQGLGWKNSYGTGAGADTIGSGLEVTWTYHPTRW
                     DNEFFHILFAYEWELTESPAGAQQWRPVNGGGADMVPEADDPSKSREPRMLTTDLSLR
                     FDPEYEKISRRFLEDPDAFSDAFARAWFKLTHRDMGPKERYLGPEVPEEDLIWQDPVP
                     AVEHELVDEVDIAALKEKILGSGLTISQLVSAAWAAAGSYRSSDMRGGANGGRIRLEP
                     QKSWAVNDPASLATVITTLEGIQADFNQASDSKQISFADLIVLAGSVAVEEAARKAGH
                     EVAVPFTPGRGDATQEQTDVESFSYLEPKVDGFRNFDARSNALPAEYRGVAREERKHR
                     A"
BASE COUNT          610 a         1105 c         1019 g          463 t
ORIGIN      
        1 gggctcttgg tcaccttgca gacgtagtgg tcgacgtcgc caccgatcgt gtcatcgaag
       61 gtgaaggtcg cgctgccgcc ggaagagacg agcagcgggc cgtcggggtc gacgggagta
      121 ggaggcggag tgatgtccgg atcagcgctg gccagcgacg gcagcgcgaa cgcagctacc
      181 acgacgaaaa gcgcggccag aaatgccagc cctcgcgaat tcattccacc cacatgcctg
      241 tcgtgtgccc ccatacactt gcgaccttac caaacagtgt tcgatcctgc acgaatttct
      301 tgccaggaag ccgaatttct cgagttatga caacccggac ctcgaccgtc ggagtcaggc
      361 ccaggggccg acgccgccga ggcgctccgg ggtgatgcga agcacccagc cgggcgcatc
      421 gattcccgga ggcggaaaga ccgcgtcggg acccatatat atgtgagcca gacgctgaag
      481 cagcgccgct gccccgccct cctcgacgcg ggcgctgccg tggaccacga gatactcctt
      541 caagcccatc tcgttggcgg aaggagactc gatcgaaagc acgatccgcg gatcccgctc
      601 gacgttcttc agcttcagcc gcttcatcat gtggccgctg acgatctcat cgccgtcgag
      661 cccgacccag acgcaggaaa gctgagggct cccgtcgcgg ttcagggtga ccaggtgggc
      721 cagggcatcc gattcgatca accgcgtcgc ttccggcgta agcacactca tcaaaccgag
      781 cctagccgac ccccggaccc acggacatcg attaggcccc ggcaggcctt aacttgatcg
      841 gttcaagaaa aggtgtataa ctaccggcgg tgccttcgac cgtcgaatac gagaacctgc
      901 tgcgcggcgc cgagcttcgc gtgacccagc cccggatggc cgtcctttcg gcggtccacg
      961 acaacccgca cgcagacacg acgacgatca tcgacgcggc ccggatccag gtgcccgaag
     1021 tctcccacca ggccgtctac gacgtgctgc gggccctgac cggcgccgga ctgatccagc
     1081 acatccagcc gccgggttcg gtggccaggt acgagtcccg ggtcggtgac aaccaccacc
     1141 acgtcgtctg ccgctcctgc ggcgcgatcg ccgacgtcga ctgcgcggtc ggctcggctc
     1201 cctgccttac ggcttccgag gatcacggct tcgtgatcga agaggcggag gtcgtgtacc
     1261 ggggcctctg ccccgactgc gcggccaaat caaccaacga gcaatccccc cgaaaggaat
     1321 cccgtgtctg aacagaacga tcagcccgac gccgtgatcg gtgaagtcaa cgaagagagc
     1381 tcgggtggct gcccggtcgt tcacgaccgg ttcccctacc cgaccgaaag cggcggcaac
     1441 atcgactggt ggccgaaccg gctcaacctg aagatcctcg ccaagaaccc ggtcgagacc
     1501 aatccctacg gcgacgactt cgattaccgg gcggagttcg aaagcctcga cctgggcgcc
     1561 gtcaagaagg acatcaacga agtcctgacc acctcgcagg actggtggcc ggccgacttc
     1621 aaccattacg gcccgctgat catccgcatg gcctggcatt cggccggtac ctaccgggtg
     1681 atggacggcc gcgggggcgg cggccacggc cagcagcggt tcgcaccgct caacagctgg
     1741 cccgacaacg tcaacctgga caaggcccgc cgcctgctct ggccggtcaa gcagaagtac
     1801 ggccgcaagc tttcctgggg cgacctgatg atcctggccg gcaacgtcgc cctcgaatcg
     1861 atgggcttca agaccttcgg tttcgccggc ggccgcgtcg acgagtggga gccggacgag
     1921 gacgtctact ggggccccga gaaggagtgg ctcggtgacg agcgctacac cggcgagcgt
     1981 gatctggaca acccgctggc cgcggtccag atgggcctga tctacgtcaa cccggaaggc
     2041 ccgaacggca acgctgaccc ggtcgccgcc gccaaagaca tccgcgagac cttcgcccgc
     2101 atggcgatga atgacgagga gaccgtcgcc ctgatcgccg gtggccacac cttcggcaag
     2161 acccacggtg ccgccgaccc ggagccctac atcggccccg agccggaagc ggccccgctc
     2221 gaggagcagg ggctcggctg gaagaacagc tacgggaccg gcgccggcgc cgacaccatc
     2281 ggcagcggcc tcgaggtcac ctggacctac cacccgaccc gctgggacaa cgagttcttc
     2341 cacatcctct tcgcctacga gtgggagctg accgaaagcc cggccggcgc gcagcagtgg
     2401 cgcccggtaa acggtggcgg agccgacatg gtgccggagg ccgacgatcc gtcgaagtcc
     2461 cgcgagcccc gcatgctgac caccgacctc tcgctcaggt tcgaccccga gtacgagaag
     2521 atctcgcgcc gcttcctcga ggatccggat gcattctcgg atgccttcgc ccgggcctgg
     2581 ttcaagctga cccatcgcga catgggcccg aaggagcgct atctcggccc ggaggtcccg
     2641 gaggaagacc tgatctggca ggacccggtt ccggcggtcg agcacgaact ggtcgacgag
     2701 gttgacatcg ccgccctgaa ggagaagatc ctcgggtccg gcctgaccat ctcgcagctc
     2761 gtctcggccg catgggccgc ggccggctcg taccgctcaa gtgacatgcg gggcggcgcc
     2821 aacggaggcc gcatcaggct cgagccgcag aagagctggg cggtcaatga cccggccagc
     2881 ctcgccacgg tgatcaccac cctggaaggc atccaggccg atttcaacca ggcgagcgac
     2941 tcgaagcaga tctccttcgc cgacctgatc gtgctggccg gcagcgtcgc ggtcgaggag
     3001 gccgccagga aggccggcca cgaggtcgcg gtcccgttca ctccgggccg tggcgacgcg
     3061 acccaggagc agaccgatgt cgagtcgttc agctacctcg aaccgaaggt ggacggcttc
     3121 cgcaacttcg acgcccgcag caacgcgctg ccggccgagt accggggcgt cgcgcgcgag
     3181 gagcgcaaac accgggc
//
LOCUS       DAOQKN010000236         3193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_19805_length_3193_cov_5.634162, whole genome shotgun sequence.
ACCESSION   DAOQKN010000236 DAOQKN010000000
VERSION     DAOQKN010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19805_length_3193_cov_5.634162"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..1273
                     /locus_tag="PLJ64_11740"
     CDS             98..1273
                     /locus_tag="PLJ64_11740"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNC94197.1"
                     /translation="MKLFRVTVLASVLAFTTAFALAGCGGSDDENAWDGQSITFGALF
                     STTGDGSPFGPQQVKGAKLAVDEINADGGINGADLQLVQRDDESDPATSADEMKELIE
                     QVQAMAVLGPTFSNSAAEADPIANQNRTPVLAVSNTGPGIVGDCDYPCEYIFRDSLGE
                     ATAIPANVKNLVDREHPKFAKVLYPVDDPFGQTSAETAVQAFKDEGVKVTSMQVGANG
                     IGHMTDERQDAFMITASSGETGAGIVKDLRNAYGAKTPILGGNAFNSPVVAESLGRQG
                     KGVQSAAAWYAGNHSDENHEFMANYMAKYGQEPDQFAAQAYTGVKLLADAAEEADLQF
                     GDIAADREALKTALEGVSEETPLGDFSFTPDHDVSQPIWIVQMNGKGTYDLVAQVNP"
     gene            complement(1477..1947)
                     /gene="rpsG"
                     /locus_tag="PLJ64_11745"
     CDS             complement(1477..1947)
                     /gene="rpsG"
                     /locus_tag="PLJ64_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNC94198.1"
                     /translation="MARRNSAVIRPLDADTKYRSRLVQQIINKVMRDGKKSTAERIVY
                     DALALVGERSGEDAVETLEEAIKQLTPALEVRSRRVGGATYQVPVEVPSRRARTLAVR
                     WLVDFARARNEYSMSERLANEILDATKQQGNAYKRKDDIFRMAQANKAFAHYRW"
     gene            complement(1947..2342)
                     /gene="rpsL"
                     /locus_tag="PLJ64_11750"
     CDS             complement(1947..2342)
                     /gene="rpsL"
                     /locus_tag="PLJ64_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNC94199.1"
                     /translation="MPNTNQLVRKGRKKPKKKVTTPGLKSGQGNKKKVAAPQRRGVCT
                     RVATVTPKKPNSALRKIARVRLAHGMEVTAYIPGEGHNLQEHSVVLIRGGRVKDLPGV
                     RYKVVRGTLDAAGVSDRKQARSRYGVKAS"
     gene            2365..2994
                     /locus_tag="PLJ64_11755"
     CDS             2365..2994
                     /locus_tag="PLJ64_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94200.1"
                     /translation="MDFAPSHPSGGHSADSAEKRPILAIDVDGVISLFGFDSPPPPEV
                     ARWELIDGTPHLISIAAAERIRRLSGRFEPVWATGWQDRANDRLSLITGIGPLPVIEF
                     DAHRKRQSGDAREPAAAAGTTEAHWKLGAIGEFCATRPLAWIDDSFDQSCFDWAAARE
                     ERGEPTLLVPTEPDLGFEEAQAAVVADWAASLASTGRSTTFGQRPDHLH"
     gene            2999..>3193
                     /locus_tag="PLJ64_11760"
     CDS             2999..>3193
                     /locus_tag="PLJ64_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94201.1"
                     /translation="MEGYWPVLFMLILLKIPAVGMIWLLYWAATNEQENGVLDEGEEG
                     GGGSKRRSRPLLPRGPRRGPH"
BASE COUNT          573 a         1043 c         1022 g          555 t
ORIGIN      
        1 ttggaggact ggcacttcgc gccctgaatg aagaacaact cagatcgatc ggaccacgga
       61 tcccgggaga gaccgtgggc aaggagggaa agacccaatg aagctgttcc gcgtcaccgt
      121 gctcgcgagc gtcctcgcgt tcaccaccgc ctttgccctt gccggatgtg gaggcagcga
      181 tgacgaaaac gcctgggacg gccagtcgat caccttcggc gccctcttct cgaccacggg
      241 tgacggctcc ccgttcggcc cccagcaggt caagggcgcc aagctcgcgg ttgacgagat
      301 caacgccgac ggcggcatca acggggccga ccttcagctg gtccagcgcg atgacgaatc
      361 cgacccggcc acctcggccg acgagatgaa ggaactgatc gagcaggttc aggcgatggc
      421 cgtgctcggc ccgaccttct ccaactctgc cgccgaagcc gacccgatcg ccaatcagaa
      481 caggactccg gtcctcgccg tctccaacac cggccccgga atcgtcggtg actgcgacta
      541 cccctgcgag tacatcttcc gcgattccct cggcgaggcc accgcgatcc cggccaacgt
      601 caagaacctg gtggaccggg agcacccgaa gttcgccaag gtgctctacc ccgtggacga
      661 ccccttcggt cagaccagcg ccgagaccgc agttcaggcg ttcaaggacg aaggtgtcaa
      721 ggtgacatcg atgcaggtcg gtgccaacgg catcggtcac atgaccgacg aacgccagga
      781 tgccttcatg atcactgcct cttcgggaga gacgggggcc gggatcgtca aggaccttcg
      841 taacgcctac ggcgccaaga ctccgatcct cggcggcaat gccttcaact ccccggtcgt
      901 cgccgagagc ctcggccggc agggcaaggg cgtccagtcc gctgccgcct ggtacgcggg
      961 caatcactcg gatgagaacc atgagttcat ggcgaattac atggcgaagt acgggcagga
     1021 accggaccag ttcgccgccc aggcctacac cggggtcaag ttgctggccg acgcggcgga
     1081 ggaagccgat ctccagttcg gtgacatcgc cgccgaccgc gaagccctca agaccgccct
     1141 ggaaggggtc agcgaagaaa ccccgctcgg tgacttcagc ttcacccccg accacgacgt
     1201 aagccagccg atctggatcg tccagatgaa cggcaagggc acctacgacc tggtcgcaca
     1261 ggtcaatccc taaccgaact ccgacccctc gacacgaagt gggtatctat ttcgtctata
     1321 tagacgaaat ccatagtcac ctcgctcctg gcgagcggga aatccgaggt gggtatgaga
     1381 ttcgttcata tggacgaaat ccatagtcac ctcactggtc tgcaatgaaa agggcggctc
     1441 ggtggccgcc cctttcgcaa gtcttgattt cgcctgctac cagcggtagt gggcgaaggc
     1501 cttgttggcc tgggccatgc ggaagatgtc gtccttccgc ttgtaggcgt tgccctgctg
     1561 cttggtcgcg tcgaggatct cgttggcgag acgctcggac atcgagtact cgttgcgggc
     1621 gcgggcgaaa tcgaccagcc agcggaccgc gagggtgcgg gcgcgccggg agggaacctc
     1681 gaccgggacc tggtaggtgg cgccaccgac gcggcgggag cgaacctcaa gggccggggt
     1741 gagctgcttg attgcctctt cgagagtctc gacggcatcc tcgccggagc gttcgccgac
     1801 cagggcgagg gcgtcgtaga cgatccgctc ggcggtcgac ttcttgccgt cgcgcatcac
     1861 cttgttgatg atctgctgga ccaggcggct gcggtacttg gtgtccgcgt caagcggacg
     1921 gatcactgcg gagttgcgac gggccattag ctcgccttca ctccgtagcg ggagcgggcc
     1981 tgcttgcggt cggaaacgcc ggccgcatcc agggtgcccc gaacaacctt gtaacgaaca
     2041 cccggcaggt ccttgacccg gccgccccgg atgaggacga cggagtgctc ctgcaggttg
     2101 tgcccctcac cggggatgta cgcggtgact tccatgccgt gggcgaggcg cacacgggcg
     2161 atcttccgca gagccgagtt cggcttcttc ggggtcacgg tggcaacacg ggtgcagacg
     2221 ccacggcgct gaggcgcggc gaccttcttc ttgttgccct gcccggactt gagacccggg
     2281 gtggtcactt tcttcttggg cttcttgcgg cccttgcgta cgagttgatt ggtattaggc
     2341 acggcgaggg atactacaca tcctttggac ttcgcgccat ctcacccctc cggcgggcat
     2401 tccgctgaca gcgccgaaaa gcgtccgatt ctggcgatcg acgtcgatgg cgtgatcagc
     2461 ctgttcggct tcgactcgcc gccaccgccc gaggtcgcgc ggtgggagtt gatcgacggc
     2521 actccacacc tcatctcgat cgcagccgcc gagcggattc gccgcctttc gggccgcttc
     2581 gaaccggtct gggcgaccgg ctggcaggac cgggccaatg accggctttc gctgatcacc
     2641 gggatcggac cgctgcccgt gatcgagttt gacgctcacc gcaagcgtca gagcggtgat
     2701 gcccgtgaac cggctgcggc agccggcacc accgaagcgc attggaaact cggcgcgatc
     2761 ggggagttct gcgccacccg gcccctcgcc tggatcgacg attcctttga tcagagctgc
     2821 ttcgactggg ccgcagcacg cgaggaaagg ggcgaaccga ccctgctggt cccgaccgag
     2881 cccgatctcg ggttcgagga ggcccaggct gccgtcgtgg ccgactgggc cgcttccctt
     2941 gcctcaaccg gccgatccac caccttcggc cagcggcccg accatttaca ctgacttgat
     3001 ggagggatac tggccagtcc tgttcatgct gatcctgttg aagatcccgg cggtcgggat
     3061 gatctggctg ctctattggg cagcgaccaa cgagcaggag aacggcgtgc tcgacgaagg
     3121 tgaagaaggc ggtggcggca gcaagcgtcg ctcccggccc ctgttgccgc gcggcccccg
     3181 gcgcgggccc cat
//
LOCUS       DAOQKN010000237         3192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_19816_length_3192_cov_5.832324, whole genome shotgun sequence.
ACCESSION   DAOQKN010000237 DAOQKN010000000
VERSION     DAOQKN010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19816_length_3192_cov_5.832324"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="PLJ64_11765"
     CDS             <1..426
                     /locus_tag="PLJ64_11765"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase"
                     /protein_id="HNC94202.1"
                     /translation="TTAPEDTSIPEPADGEAEKVARDYLVEVSGKRFDVKVIGEATGF
                     AAAGAAPGAGGPKPKREKRAGGGGGGGGPVLASPLQGSIFKLEVAEGAEVAEGDLICV
                     IEAMKMENEITAHKAGKVTKVSISVGDAVASGDPLVTIE"
     gene            499..723
                     /locus_tag="PLJ64_11770"
     CDS             499..723
                     /locus_tag="PLJ64_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94203.1"
                     /translation="MHIFAYLDPGSVSAALAAVLAGVAGIGAAIRTFGSNLKSKLMFW
                     KKDEPAPEAAATATSAAPTDAPAEDKPAEQ"
     gene            728..2134
                     /locus_tag="PLJ64_11775"
     CDS             728..2134
                     /locus_tag="PLJ64_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94204.1"
                     /translation="MAATEPGSFRDRDSRVVVSGDAIYRALSPEGAEDWEAFSASPLL
                     EQLTAAGKVIGTREVDPSVLGESQDLLPTGITKVLEHDRVPFVSYPYEWTFSMLQDAA
                     KLQLELGAAAIDSGLGLKDATPYNVQFIGSQPTFIDIGSFEKITEGEPWIAYRQFCML
                     YLYPLLFQAHKDIPFHPWMRGSIDGIQPIDAIKVFSLRDRLRRGVFLHTSLHARLDRR
                     ANKSGPGSAEENKTKRQVKPGQIKAQLESMNRLVSKLKWKAGETSWSGYRQSNTYSDE
                     DDRRKQAFVGEVAAQLKPGLTWDMGCNDGAYSRIAAESSERVVAFDFDHATVEALYRS
                     LREEGNTKILPLVSNLADPSPALGWRGLERKTLADRGAPDLMLALALIHHVSISANIP
                     IADFLQWARDLETTLLVEFPKRTDPMVRALLANKHEGANPDYEEGNFERELEKRFEVE
                     RREELPSGDRILYLARPR"
     gene            2166..>3192
                     /locus_tag="PLJ64_11780"
     CDS             2166..>3192
                     /locus_tag="PLJ64_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94205.1"
                     /translation="MEPRKGLAAVFDRPAPTGRQLLLGGGHLAALWALAFVQPLLDLL
                     GNNPDFFVARGNSTGDILILAIGFTLLPPLVLFGIEWVVSKVNARAYYGLHLILMGLI
                     ATFFFVPLVSDVFTARSIIILVVSLGLGALLAWMVFRFVFVKNLMDILIIAPIVVLAL
                     FIFNSKTTDLIFPNEGEFKVSADSGNDTPVVLMIFDELGTSNLMTRDGKIAAGRFPNF
                     ARLARSSTWYPNETTTAFFTPHAVPGILTGINQDADTLPTWQEQPLSMFSQFSKGRQL
                     HVLEPVTAICPEDLCPDTEADAGQLTRLKSLFQDLKIVEGKLDLPPGLARKLPDVSSN
                     FEGFGEGS"
BASE COUNT          578 a         1108 c          987 g          519 t
ORIGIN      
        1 accaccgccc ccgaggacac ttcgatcccc gagccggctg acggcgaggc cgaaaaggtc
       61 gcccgcgact acctggtcga ggtttctggc aagcgctttg acgtaaaggt gatcggcgaa
      121 gcgaccggct ttgccgccgc cggcgccgcg cccggagccg gaggacccaa gccgaagcgc
      181 gagaagcgcg ccggtggcgg aggcggcgga ggcggccccg tgcttgcctc accgctccag
      241 ggatcgatct tcaagctcga agtcgccgaa ggcgccgagg tggctgaagg cgacctgatc
      301 tgcgtgatcg aagcgatgaa gatggaaaac gagatcaccg ctcacaaggc cggaaaggtc
      361 accaaggtca gcatttccgt tggcgatgcc gtcgcgtccg gggaccctct ggtcacgatt
      421 gagtaacatt tcgcggtcgg tcggggcctg ctcaggaaag tcaggcaacg gtcgatcccg
      481 cccacgagag gatcacgaat gcacattttt gcctatctgg accccggcag cgtcagcgct
      541 gccctcgccg cagttctggc cggagttgcc ggaatcggcg ccgccattcg cactttcggc
      601 agcaatctga agagcaagct catgttctgg aagaaggacg agcctgcccc ggaggccgcc
      661 gccaccgcca cgtcggcggc ccccaccgac gcccccgccg aagacaagcc cgccgagcag
      721 tagggctgtg gcggccacag aaccgggctc gttcagggac cgggacagcc gcgtcgtcgt
      781 ttcaggcgac gcgatctacc gggccctgag ccccgaaggc gccgaggact gggaagcgtt
      841 ctccgccagc cccctgctcg aacagctgac cgccgccggc aaggtgatcg gcacccggga
      901 ggtcgacccc tcggtcctcg gcgaaagcca ggacctcttg cctaccggga tcaccaaggt
      961 cctcgagcat gaccgggtcc ccttcgtttc ctatccctac gagtggacct tcagcatgct
     1021 gcaggacgcc gcgaagcttc agctcgagct cggcgcagcc gcgatcgatt ccggcctggg
     1081 cctcaaggac gccaccccct acaacgtcca gttcatcggc tcgcagccga ccttcatcga
     1141 catcgggtcc ttcgagaaga tcaccgaggg cgagccgtgg atcgcctacc ggcagttctg
     1201 catgctctac ctctacccgc tgctcttcca ggcccacaag gacatcccct tccatccctg
     1261 gatgcgcggc tcgatcgacg gaatccagcc gatcgacgcg atcaaggtct tctcgctgcg
     1321 tgaccgcctg cgccgcggcg tcttcctcca cacttcgctc cacgcgaggc tggaccgccg
     1381 cgccaacaag tccggccccg gcagcgccga ggagaacaag accaagaggc aggtcaagcc
     1441 ggggcagatc aaggcccagc ttgaatcgat gaaccgcctg gtctcgaagc tgaagtggaa
     1501 ggccggcgag acctcatgga gcggctaccg ccagtcgaac acctactcgg acgaggatga
     1561 ccggcgcaag caggccttcg tcggcgaagt cgcggcgcag ttgaagcccg gcctgacctg
     1621 ggacatgggc tgcaacgacg gcgcctactc gcggatcgcc gccgagagct ccgaacgggt
     1681 ggtcgccttc gacttcgacc atgccaccgt cgaggcgctc taccggtcac tgcgggaaga
     1741 aggcaacacg aagatcctgc ccctcgtctc gaacctggcc gacccttcac cggcgctcgg
     1801 ctggcgggga ctggaacgga agaccctggc cgaccgcggc gccccggacc tgatgctggc
     1861 cctggccctg atccaccacg tttcgatcag cgccaacatt cccatagccg acttcctgca
     1921 gtgggcacgg gacctggaga ccacgctcct ggtcgagttc ccgaagcgga ccgacccgat
     1981 ggtgcgggcc cttctggcca acaagcacga aggagccaac cccgattacg aggaaggcaa
     2041 cttcgagcgc gagctggaaa agcggttcga agtggagcgc cgcgaggagc tgccttccgg
     2101 cgaccggatc ctctacctgg cccggccgcg gtaggagttt gagcgctccg ggcgaaagtt
     2161 cggaagtgga accgaggaag ggattggcgg cggtcttcga ccggccggcc ccgaccgggc
     2221 gtcagttgct gctcggcgga ggccatctgg cggcgctctg ggcgctcgcc ttcgttcagc
     2281 cgctgctcga cctgctgggc aacaaccccg acttcttcgt cgctcgaggc aactcgaccg
     2341 gtgacatcct gatcctcgcg atcggcttca ccctgctccc gccgctggtc ctgttcggga
     2401 tcgagtgggt ggtcagcaag gtcaacgccc gggcctacta cggcctgcac ctgatcctga
     2461 tggggctgat cgccaccttc ttcttcgttc ccctggtctc cgatgtcttc acggcccggt
     2521 cgatcatcat cctggtcgtg tcactgggcc ttggggcgct cctcgcctgg atggtcttcc
     2581 gcttcgtctt cgtcaagaac ctgatggaca tcctgatcat cgcgccgatc gtcgtcctgg
     2641 ccctgttcat cttcaacagc aagacgaccg acctgatctt ccccaacgag ggcgagttca
     2701 aggtctcggc cgattccggc aacgacaccc cggtcgtact gatgatcttc gacgagctcg
     2761 gcacctcgaa cctgatgacc agggacggga agatcgccgc cggccgcttc cccaacttcg
     2821 cccggctggc ccgcagcagc acctggtatc cgaacgagac gacgaccgcc ttcttcaccc
     2881 cccacgcggt gccgggaatc ctgaccggga tcaaccagga tgccgacacc ctgccgacct
     2941 ggcaggagca gccgctcagc atgttcagcc agttcagcaa gggtcgccag ctccacgtgc
     3001 tggaaccggt cactgcgatc tgcccggagg atctctgccc ggacacggaa gccgatgccg
     3061 gccagttgac ccggctcaag tcgctcttcc aggacctgaa gatcgtcgaa ggcaagctcg
     3121 acctgccgcc cggacttgcc cgcaagctgc cggacgtgag ttcgaacttc gaggggttcg
     3181 gcgaaggaag cc
//
LOCUS       DAOQKN010000238         3185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_19891_length_3185_cov_5.655911, whole genome shotgun sequence.
ACCESSION   DAOQKN010000238 DAOQKN010000000
VERSION     DAOQKN010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19891_length_3185_cov_5.655911"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="PLJ64_11785"
     CDS             <1..746
                     /locus_tag="PLJ64_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94206.1"
                     /translation="PGEPAGDEETREYETVATGEGEPVDREEAIEPVEEEEETAEALD
                     EAETGVIEEDTEVIEEEDTEVIEEDDTEVIEEEAWDDEGYEDWEQEAEEVEEEPDPEA
                     KAEPENSGGFAVWFTGFRQRANEVWSGFWGRIGSIKLPRHELNGRKVLAVAGVLAIAL
                     MVGAGGYLLGKGSGDDVDQARLEGEFAGRKAGAIAGATRGYAAGFKKGRDLAFRKSYS
                     ASYKRNYIRAYQDAGMDPPKPEDVEVPEP"
     gene            743..1264
                     /locus_tag="PLJ64_11790"
     CDS             743..1264
                     /locus_tag="PLJ64_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94207.1"
                     /translation="MNYDRISPWLISGVLAVLMAGLAGWAIGAGAANGESDASAARKD
                     GFREGFALVFPDARSATAGRGFKEGVHRGRTAGAKTGSREGATIGAGNVEIEQAVDSE
                     KAAEAAASAAQSEIAARQPNCGIVPDAPSWCPTSDELASYKAAVKAAEEAAKEAEKQK
                     QEDQKPGKANGFP"
     gene            1299..1676
                     /locus_tag="PLJ64_11795"
     CDS             1299..1676
                     /locus_tag="PLJ64_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94208.1"
                     /translation="MIPALAGVALLLAAAGIGFGIGKTTVSRDPGPDLTRAEAFVQAR
                     KKAEKEVSRQMARRGFDAGRRSGHSHGIIAGGMAAESAVTIQFRQQRASVAQSDAAEV
                     QSELAGMTAAPAPPVPTPEPDDG"
     gene            complement(1684..2616)
                     /locus_tag="PLJ64_11800"
     CDS             complement(1684..2616)
                     /locus_tag="PLJ64_11800"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNC94209.1"
                     /translation="MSGSADPKDQHKDATGSAIAGLPVVFGIWLVSVSFVFFPLSLLL
                     TDWLGDPAGATPGEMLGDPLFLSIFLAVTGLWTLFAPLALTGWADRTVWMKRLGCREL
                     TPEERSRLDPLWREVFLYAGIHPEAYRLKMSDKPVQNAFAVGDGMITLDQGILSMDDA
                     DLRAIIGHELGHHVRRHTQINMLALWFGAPARILYGILLFLVAGLIRLAGALSGCSWA
                     IIVLLVAFALAVPAIVLFLLLGIAQLLTRLLDRKSEFEADLYSAGIGYRQEMIGALTE
                     LREIYGDPEPNKPWSPARLGSTHPPFTDRIAALG"
     gene            complement(2662..>3185)
                     /locus_tag="PLJ64_11805"
     CDS             complement(2662..>3185)
                     /locus_tag="PLJ64_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94210.1"
                     /translation="VRPGPYTLKLLQDGIEVDSVTVTGDSYSHTFNVSESGRYGFELT
                     RPLGDQTMIEAYSTPVWFTFKKPSNTFSFGAYKANRAKGTATLKVKVVSPGSVKLTGP
                     GLNNASAKVTKANQTVTLNLKPKASLKKQLKKKGSAKVKVKVTNTPTGGSAASKSKTV
                     KLLGKKAKKKKRH"
BASE COUNT          598 a          984 c         1123 g          480 t
ORIGIN      
        1 tgcccgggga acccgccggg gacgaggaaa ccagggagta cgaaaccgtc gcgaccggcg
       61 aaggggaacc ggtagatcgg gaagaggcca tcgagccggt cgaagaagaa gaagagaccg
      121 ccgaagctct cgatgaggcc gagaccgggg taatcgaaga agacaccgag gtgatcgaag
      181 aggaagacac cgaggtaatc gaggaagacg acaccgaggt aatcgaagag gaagcctggg
      241 acgacgaggg atacgaagac tgggagcagg aagcagagga agtagaagag gaaccggatc
      301 cggaggcgaa ggccgaaccg gagaattcag gtggtttcgc ggtctggttc accggtttca
      361 ggcagagggc gaacgaggtc tggtccggat tctggggtcg gatcggttcg atcaaactgc
      421 cccggcatga gctgaacgga cggaaggtcc tcgccgtggc gggggttctg gcgatcgcgc
      481 tgatggtcgg tgccggtggc tatctgctcg gcaagggttc cggcgacgat gtcgaccagg
      541 cccggctcga gggcgagttc gccggccgca aagccggcgc catcgccggg gcgaccaggg
      601 gctacgcggc cggtttcaag aagggtcgtg acctcgcctt ccgcaagtca tattcggcct
      661 cctacaagcg caactacatc cgggcctacc aggacgccgg gatggacccg ccgaagccgg
      721 aagacgtcga agtccccgaa ccatgaacta cgaccggatc tccccctggc tgatctcagg
      781 cgtgctggcc gtcctgatgg ccgggctggc cggctgggct atcggggcag gggcggcaaa
      841 cggcgagagt gacgccagcg ccgcccgcaa ggacggtttt cgggaaggat tcgcgctggt
      901 cttccccgac gcacggtcgg ccaccgccgg tcgggggttc aaggagggcg tccaccgggg
      961 aaggacggcc ggtgcgaaga ccggctcccg ggaaggagcc acgatcggcg ccggcaacgt
     1021 cgagatcgag caggcggtcg attcggagaa ggccgccgag gcggccgcgt cggcggccca
     1081 gtcggagatt gcggcccgcc agccgaactg cgggatagtc ccggacgcgc cttcctggtg
     1141 cccgaccagt gacgaactcg cttcctacaa ggccgcggtc aaggcagccg aggaagcagc
     1201 caaggaagca gagaagcaaa agcaggaaga tcagaagccg ggcaaggcca atggattccc
     1261 ctgaaccgga agaaagcgaa ccggggggaa ggccctggat gattcccgcc ctggccggtg
     1321 ttgccctgtt gctcgccgcg gcggggatcg gtttcgggat cggcaagacc accgtttccc
     1381 gtgatcccgg gcctgacctg acccgggccg aggccttcgt tcaggcccgg aagaaagcgg
     1441 aaaaggaggt gagccgccag atggcccggc gcggcttcga cgcaggccgc cgttccggcc
     1501 acagccacgg gatcatcgcc ggcgggatgg ccgccgaatc cgccgtgact atccagtttc
     1561 gccagcaacg cgcgtcggtc gcccagtccg acgcagccga ggtccagtca gagctcgccg
     1621 ggatgaccgc cgcccccgcg ccgccggtcc cgactcccga acccgacgac ggctgacccc
     1681 ggctcagccc agggcggcga ttcgatcggt gaagggcggg tgggtggagc ccaggcgggc
     1741 cggtgaccac ggtttgttgg gttccgggtc accgtagatt tcacgcagtt cggtcagggc
     1801 cccgatcatc tcctggcgat agccgatccc ggccgagtag aggtcggctt cgaactcgga
     1861 cttgcggtcc agcagccggg tcaggagctg ggcgatgccg agcagcagga acaggacgat
     1921 cgccggaacc gcaagcgcga aggcgaccag cagcacgatg atcgcccagc tgcagccgga
     1981 aagggccccg gcgagccgga tcaatccggc gacgaggaaa agcaggatgc cgtagaggat
     2041 ccgggccgga gcaccgaacc agagagcgag catgttgatc tgggtgtggc gccggacgtg
     2101 gtggccaagt tcgtggccga tgatcgcgcg gaggtccgcg tcatccatgc tgaggatccc
     2161 ctggtccagg gtgatcatgc cgtcgccgac cgcgaacgca ttctgtaccg gcttgtcgct
     2221 catcttcagc cggtaggcct ccggatggat gccggcgtag aggaagactt cccgccagag
     2281 cgggtcgagc cgggatcgct cttccggggt cagttcccgg cagccgagtc gcttcatcca
     2341 gacggtgcga tccgcccagc cggtcagggc cagcggcgcg aacagcgtcc acagcccggt
     2401 cacggccagg aatatcgaca ggaacaacgg gtcgcccagc atttcgcccg gcgtcgcccc
     2461 ggccgggtcg ccaagccagt cggtcagaag cagcgaaagc gggaagaaga cgaaggaaac
     2521 cgagaccagc cagatcccga acacgaccgg caggccggcg atcgccgacc ccgtcgcgtc
     2581 cttgtgctga tccttcggat cggccgatcc gctcaccggt cgatcccggg gttgcgctgg
     2641 ctgcccctgg gccggccccg gctagtgccg cttcttcttt ttcgccttct tgccgagcag
     2701 cttgaccgtc ttcgatttcg aggcggccga tccgccggtc ggggtattgg tcaccttgac
     2761 cttcaccttg gccgagccct tcttcttcag ctgcttcttc agcgaagctt tgggcttcag
     2821 gttcagggtc accgtctggt ttgccttggt caccttggcc gacgcgttgt tcaacccggg
     2881 gccggtcagc ttgacgctgc cggggctcac aaccttgacc ttgagggtcg cggtcccctt
     2941 ggcccggttc gccttgtagg cgccgaagct gaaggtattc gacggcttct tgaaggtgaa
     3001 ccagaccggg gtcgagtagg cctcgatcat cgtctgatcg ccgaggggcc gggtcagctc
     3061 gaacccgtag cggcccgact cggagacgtt gaaggtgtgg ctgtaactgt caccggtgac
     3121 cgtgacggaa tcaacctcga tgccgtcctg cagcagcttc agcgtgtagg gacctgggcg
     3181 aaccg
//
LOCUS       DAOQKN010000239         3177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_19979_length_3177_cov_6.202434, whole genome shotgun sequence.
ACCESSION   DAOQKN010000239 DAOQKN010000000
VERSION     DAOQKN010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19979_length_3177_cov_6.202434"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="PLJ64_11810"
     CDS             <1..919
                     /locus_tag="PLJ64_11810"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC94211.1"
                     /translation="TLLAVGAAVAVTAASIPAVHSGELNGVLLAALALLAMASFEAIT
                     PLSQAAAVIDATDEAAERIEEVSERPVPILNPEHPRPVPAGGPIRLDGVSFAYPGGDG
                     LLLRDADLEIRPGEAVALIGPSGIGKSTLSELLVRFRDPDRGRITLGGADIRDLDQEE
                     LREAVRLAPQDAYMFNTTIAENVKLGRPEADRERIVEALAEVGLEDWINSLPDGIDSL
                     VGEDGSMISGGQRQRIAAARVFLSRARFLIFDEPTAHLDPDGAAALESHLVDRRDQGC
                     GILVITHTITDPQAFDRVLELRDGTFREV"
     gene            complement(997..2157)
                     /locus_tag="PLJ64_11815"
     CDS             complement(997..2157)
                     /locus_tag="PLJ64_11815"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNC94212.1"
                     /translation="MNEATVSLDEARLRRLIEVGRSLVVESDPEAVINDALEAARELT
                     GARYAALGVLDSRRSELEQFLTRGIEAKLREQIGHPPHGGGVLGLLIEEPEPLILDDV
                     SLHPRSQGFPPGHPEMKSFLGVPVKIRGEAWGNLYLTEKEEGTFDDADIQSVVILAEW
                     IAIAIDNARSAAAERVRLTIEAAEQERGRWARELHDETLQNLAGLRVLLSGTRRRTAM
                     GPVSPILDQAIERIDETIVELRRLIADLRPAALDELGVEAALSSLVERLSSNGVEIDL
                     YVELRPEDEMGGRYEPLLEDTVYRLVQESLNNAIRHGHAVRAIVEVIEEEGEIRIRVI
                     DDGSGFEPNRAGGGGFGLLGMRERVNLTGGHFDVRSGEDGTTITASLPARQP"
     gene            2268..2927
                     /locus_tag="PLJ64_11820"
     CDS             2268..2927
                     /locus_tag="PLJ64_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC94213.1"
                     /translation="METGAESAAEKLTLVLVDDHVVVRSALKVLLESEPDLEVVAEAG
                     TADDAIRYVGAHKPDVLVLDLNMPGRPSLEAIPDIQAASPDTKIVILTMRNEPAFARQ
                     ALQAGVMGYVLKEAADTELVLAVRRAADGDTYLQPSLYQHLADKDKAEGRTGRTLSDR
                     EVEVMRLIALGHTNAEIADRLFLSVRTVESHRASIQKKLDIGSRAELVRYALDQGLIS
                     A"
     gene            2993..>3177
                     /locus_tag="PLJ64_11825"
     CDS             2993..>3177
                     /locus_tag="PLJ64_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94214.1"
                     /translation="MLILLPPSEGKASPSGMSCLDLGSLAFAEYLSPKRAELIAALEK
                     LGKAPEKKGIEALGISKG"
BASE COUNT          521 a         1069 c         1055 g          532 t
ORIGIN      
        1 aaccctgctg gccgtaggcg ccgcggttgc cgtcaccgcc gcctcgattc cggctgtcca
       61 ttcgggtgag ctgaacggcg tgctgctggc cgccctggct ctgctggcaa tggcctcctt
      121 cgaggcgatc accccgctca gccaggcggc ggccgtgatt gacgcgaccg acgaagcggc
      181 ggaacgcatc gaggaagtgt ccgagcgtcc ggtacccatc ctcaaccctg aacatcccag
      241 accggtgccg gccggcggcc cgatcaggct ggacggcgtg agtttcgcct acccgggagg
      301 ggacggcctg ctgctgcgcg acgccgatct ggaaatccgg cccggcgagg cggtcgccct
      361 gatcgggccc agcgggatcg gcaagagcac cctttccgaa ctcctcgttc gcttcaggga
      421 tccggaccgt ggacggataa cgcttggcgg cgcggacatc cgtgacctgg accaggagga
      481 gttgcgcgaa gcggtccggc tggccccgca ggacgcttac atgttcaaca ccacgatcgc
      541 cgagaacgtc aagctgggca ggccggaagc ggaccgcgag cggatcgtcg aagccctggc
      601 cgaggtcggc ctcgaggact ggatcaactc gctgcccgac gggatcgatt ccctggtcgg
      661 cgaggacggc tcgatgatct ccggcggcca acgccagcgc atcgccgcgg cccgcgtgtt
      721 cctttcccgg gcccgctttc tgatcttcga cgagcccacc gcccatctcg acccggacgg
      781 tgccgccgcc ctggaaagtc acctggtcga tcgccgcgat cagggctgcg gcatcctggt
      841 catcacccac acgatcaccg acccacaggc cttcgaccgg gttctcgaac tgcgagacgg
      901 aaccttccgc gaagtctgac cgggcgccgg gaccgggttc gctcgcgaca ctggcgatcg
      961 ggatcgattc gatgcgtccc gccgggcggt gcgagttcag ggctgccggg caggcaacga
     1021 ggcggtgatc gtggtgccat cctcgcccga gcgcacgtcg aagtggccac cggtcaggtt
     1081 gacccgctcg cgcatgccga gcaggccgaa cccgccgccg ccggcccggt tgggctcaaa
     1141 gccggagccg tcgtcgatca ccctgatccg gatctctcct tcttcttcga tcacctcgac
     1201 gatcgcccgc acggcatgtc cgtgccggat cgcattgttg agtgactcct ggacgagccg
     1261 gtagacggtg tcttcgagca gcggctcgta gcggccgccc atttcgtctt cggggcggag
     1321 ctcgacataa aggtcgatct cgaccccgtt gctggaaagc ctttcgacca gtgagctgag
     1381 cgccgcttcc acgccgagtt catcgagggc ggccggccgg aggtcggcga tcagccggcg
     1441 cagttcgacg atcgtttcgt cgatccgctc gatcgcctgg tcgaggatgg gggagaccgg
     1501 tcccatcgcg gtgcgtcgcc gggtgccgga aagcagcacc cggaggccgg ccaggttctg
     1561 aagggtctcg tcgtgaagct cgcgggccca gcgtccgcgt tcctgctcgg ccgcctcgat
     1621 tgtcaggcgc acccgttcag cggcggccga cctggcattg tcgatcgcga tcgcgatcca
     1681 ttcggcgagg atcaccaccg actggatgtc ggcatcgtcg aaagtgccct cttccttctc
     1741 ggtcaggtag aggttgcccc aggcttcgcc gcggatcttc accggaaccc caaggaagga
     1801 cttcatctcc gggtggccgg gaggaaaccc ctgcgatcgg gggtggaggc tgacgtcgtc
     1861 gagaatcagc ggctccggtt cctcgatcag cagaccgagc acgccgccgc catggggagg
     1921 atggccgatc tgttcccgca gtttcgcctc gatgccgcgg gtcaggaact gttcgagttc
     1981 ggagcggcgg ctgtcgagaa ctccgagtgc cgcgtagcgc gccccggtca gttcgcgggc
     2041 cgcctccagc gcgtcgttga tcaccgcttc cgggtcggac tcgacgacca ggctgcggcc
     2101 gacttcgatc aaccggcgca ggcgagcctc atcaagactg acggtcgctt cgttcacgcc
     2161 ggggattgta gaggcaggcc tgccggattc cgctcccctc cctggtaaat cgaagtctgc
     2221 ggaaaggtac ggaaatggaa cccgccccgg cgggtagcct tcccttgatg gaaacgggtg
     2281 cggaaagcgc tgcggagaag ctgactctcg tgctggtcga tgatcacgtc gtcgtgcgat
     2341 cggccctcaa ggtcctgctc gaatccgaac ccgacctgga ggtggtggcc gaggccggca
     2401 ccgccgatga cgcgatccgt tacgtcggtg cccacaaacc ggatgtactg gtgctcgacc
     2461 tcaacatgcc gggccgcccc agcctcgagg cgatcccgga cattcaggcc gcctccccgg
     2521 acacgaagat cgtgatcctg accatgcgaa acgagcccgc tttcgcgcgg caggcccttc
     2581 aggccggggt catgggatac gtgctcaagg aggccgccga caccgaactg gtcctcgcgg
     2641 tccggcgcgc ggccgacggc gacacctatc tccagccttc cctctaccag cacctggccg
     2701 acaaggacaa ggccgaaggc cgaaccggcc gtaccctgag cgaccgcgag gtcgaggtaa
     2761 tgcgcctgat cgcgctcggc cacaccaacg cggagatcgc cgaccggctt ttcctcagcg
     2821 tccgcacggt ggaaagccac cgggccagca tccagaagaa gctcgacatc ggcagccgcg
     2881 ccgaactcgt gcgttacgca ctggatcagg ggctgatcag cgcgtaacgc gcttgacggg
     2941 agtcggcacc gcccagggat ggttgggagg gctccgtcgg tagattcacg gcgtgctgat
     3001 cctgctacct ccttccgagg gcaaggcttc tccctcggga atgtcttgtc tcgatctcgg
     3061 ctcactggcc tttgccgagt acctgagccc gaagcgggcg gagttgatcg ctgctctgga
     3121 gaagctgggc aaggccccgg agaagaaggg catcgaggcg ctgggcattt cgaaggg
//
LOCUS       DAOQKN010000240         3095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_20928_length_3095_cov_3.675658, whole genome shotgun sequence.
ACCESSION   DAOQKN010000240 DAOQKN010000000
VERSION     DAOQKN010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20928_length_3095_cov_3.675658"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..2128
                     /locus_tag="PLJ64_11830"
     CDS             119..2128
                     /locus_tag="PLJ64_11830"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HNC94215.1"
                     /translation="MSLGAGLPDAQGSAYMNGDSVTTDSNGIAHYLVFGPVNYLSGTA
                     VPNLPGLNNAGFNIYPYVPGTTVALVFPATDHLAPQAHLNSPAEGATYTQGQSVPADY
                     TCEDETGGSGISRCEGTTANGQPIDTSSLGPHSFDVLAVDRAGNSSTVTHHYMVVPGG
                     VADLTIEKTATPANPRPGDTVTYTLTALNQGNNVARDVSITDALPAGVSFVSATAPCL
                     EAASTVTCEIGSLAAGEQRSYEIRVTVDQWGNANPEATHLLDVQKAEAQIDLNAGEQR
                     TVSVSCPGGYFVADGSVRIDHVDQGSGDWDSPEVLESRAVSNDTWRGTVRNTASGRAQ
                     AKVFAVCIRQQTNDAGGHSHNLIVSAPVTVTSPAAAGLHEAVLQCGPGQIAVAPGFES
                     SAPGDLVYSQPEGNGWKFRLGLNAPASVTFQIRCLTRQVSSSGGHTHDLALERISHEF
                     TVGPGQVNEAQLTCADGSKGIVAGWRLDQDLLSLGNDPRPVTRAFRLYNPTDHDLTAT
                     LSLLCLGDLTGGEHLAARTITNTAGISTSSNEIVTGNNTGSATITVEDTDNYTPVTEP
                     DTGKPTPNNPTGGPIANRPAPTPIARITARPRRRTFSRNATFRFNSTVVGSTFECRID
                     NQPYAPCSSPKVYSNLARRGHTFRVRATAAGVTGPATVYQWRIRQ"
     gene            complement(2148..2747)
                     /locus_tag="PLJ64_11835"
     CDS             complement(2148..2747)
                     /locus_tag="PLJ64_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNC94216.1"
                     /translation="MTTQKVTADMTITLDGFASGRGQSRERPFGDLDHKRLHHWMFEH
                     REDNLAEAEAIVEAGAFIMGRKMFSAEEGNEWDLDWTGWWGSDPPYHAPTFVLTHLPR
                     ASIEMEGGTTFHFVTDGIEAAMEMAREAAGERNVSIAGGPGTLNQYLAAGLVDELRLH
                     VSPFVAGAGERIFTGAEDFDLKPLSSRATPHVTHLVYGR"
     gene            complement(2744..>3095)
                     /locus_tag="PLJ64_11840"
     CDS             complement(2744..>3095)
                     /locus_tag="PLJ64_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94217.1"
                     /translation="PGMTLVQRGHMPPPEPGAPGIFAMADPARINDLVTGAGFPEPEI
                     QEIAFTFDYPDSDDVWEAIVRIAGPLAEVINHLDEDEREATRAAILENLAGFRNEDGS
                     YSAPAASWGIVSGR"
BASE COUNT          543 a         1096 c          975 g          481 t
ORIGIN      
        1 caggtcggtc aagccggtcg ccccggaggt gacgtagccg agtttcgcgt ccacggcctc
       61 ctgcatggcg tcgccggggt ggccgttcag caacgaggta cctgccacca gcaacgcgat
      121 gagcctcggt gccggcctgc ccgacgcgca gggttcggcc tacatgaacg gtgactcggt
      181 gaccaccgat tcgaacggga tcgcccacta cctggtgttc ggcccggtca attacctgag
      241 cggcaccgcc gtccccaacc tgcctggcct caacaacgcc ggcttcaaca tctacccgta
      301 tgtgcctgga accacggtgg cactcgtctt ccccgccacc gaccatctcg cgccgcaggc
      361 ccacctgaac agccctgccg agggagccac ctacacccag ggccagagcg ttcccgccga
      421 ttacacctgc gaggacgaaa ccggcgggtc gggaatcagc cgctgcgagg gaaccaccgc
      481 aaacggccag ccgatcgaca cttcgtcgct cgggccccac agcttcgatg tgctcgcggt
      541 cgaccgggcc ggcaacagct cgaccgtgac ccatcactac atggtggtac ccggcggcgt
      601 ggccgacctg acgatcgaaa agaccgctac cccggcgaac ccgcgccccg gtgacaccgt
      661 cacctacacg ctgaccgccc tgaaccaggg caacaacgtg gcccgggacg tgtcgatcac
      721 cgatgccctg ccggcggggg tctcgttcgt ctcggccacc gccccctgcc tcgaggctgc
      781 ctccaccgtc acctgcgaaa tcggttcgct cgcggccggc gagcaaaggt cgtacgagat
      841 ccgggtgaca gtcgaccagt gggggaacgc caaccccgag gccacccatc tgctcgatgt
      901 gcagaaggcg gaagcccaga tcgacctcaa cgccggcgag cagcgcacgg tttcggtgag
      961 ctgcccgggc ggctacttcg tcgccgacgg ctcggtccgg atcgaccatg tggaccaggg
     1021 cagcggtgac tgggactccc cggaagtgct cgaatcgagg gccgtctcaa acgacacgtg
     1081 gcgcggcacc gtccggaata cggccagcgg cagggcccag gcgaaggtct ttgccgtgtg
     1141 catcaggcag cagacgaacg acgccggcgg ccacagtcac aacctgatcg tttcggcacc
     1201 ggtgaccgtg accagcccgg cagccgccgg gctgcacgaa gccgtcctgc agtgtggccc
     1261 cgggcagatc gccgtggccc cgggtttcga gtcctccgct ccaggcgacc tcgtctacag
     1321 ccagcccgag ggcaacggct ggaagttccg cctcggcctg aacgcaccgg ccagcgtgac
     1381 cttccagatc cgttgcctca cccgtcaggt gtcgagttcc ggcgggcaca cccacgacct
     1441 cgccctcgag cggatatcgc atgagttcac ggtcgggccg ggtcaggtca acgaagcgca
     1501 gctgacctgc gccgacggca gcaaggggat cgtcgccgga tggaggctcg accaggatct
     1561 gctctcgctg ggcaatgacc cgaggccggt gaccagggcg ttcaggctct acaacccgac
     1621 cgaccatgac ctgaccgcaa cgctcagtct gctctgcctc ggcgacttga ccggtggcga
     1681 gcacctggcc gcccggacca tcaccaacac ggccggaatc tcgacttcct cgaatgagat
     1741 tgtgaccggc aacaacacgg gatcggccac gatcaccgtc gaggacacgg acaactacac
     1801 cccggtcacc gagcccgaca ccggcaagcc aaccccgaac aatccgaccg gcgggccgat
     1861 cgccaaccgt cccgccccga ccccgatcgc aaggatcacc gcccggcccc gccggcggac
     1921 cttctcccgc aacgccacct tccgcttcaa ctcgaccgtg gtcggctcca cctttgaatg
     1981 ccggatcgac aatcagccct acgcgccgtg cagctcgcca aaggtctatt cgaacctggc
     2041 caggcgcggg cacaccttca gggtcagggc aaccgccgcc ggggtgacgg gcccggccac
     2101 cgtgtatcaa tggaggatca ggcagtaggg cgcgaagcct cctcgggtca ccggccgtag
     2161 accaggtgag tcacatgcgg ggtggcgcgg gagctgagcg gtttcaggtc gaagtcttcg
     2221 gcaccggtga agatcctttc tcccgctccc gcgacgaagg gggagacgtg aaggcggagc
     2281 tcgtcgacca gaccggccgc caggtactgg ttgagcgtgc ccgggccgcc ggcgatcgag
     2341 acgttcctct caccggcagc ctcccgggcc atctccatcg ccgcttcgat tccgtcggtg
     2401 acgaagtgga aggtggtgcc gccctccatt tcgatgctgg cccggggcag gtgagtgaga
     2461 acgaaggtcg gcgcgtggta gggagggtcc gaaccccacc agccggtcca gtcgagatcc
     2521 cactcgttgc cttcctcggc cgagaacatc ttgcggccca tgatgaaagc tccggcctcg
     2581 acgatcgcct cggcctctgc gaggttgtcc tcgcgatgtt cgaacatcca gtggtggagc
     2641 cgcttgtggt cgaggtcgcc gaagggccgc tcccggctct ggccccgccc gctcgcaaag
     2701 ccgtccaggg tgatcgtcat gtcggccgtg actttctggg tggtcatcgg ccggacacta
     2761 ttccccagct cgccgccggg gccgaatagg aaccatcttc attgcgaaac cccgccaggt
     2821 tttccaggat ggcggcccgt gtcgcctctc gctcgtcttc gtcaaggtgg ttgatcacct
     2881 cggcgagcgg cccggcgatt cgcacgatcg cctcccagac atcgtcggaa tccgggtaat
     2941 cgaaagtgaa ggcgatctcc tgaatttccg gttccggaaa cccggctccg gtgaccagat
     3001 cattgatccg ggcagggtcg gccatggcga agattccggg tgcccccggt tcggggggag
     3061 gcatgtggcc ccgctggacc agggtcattc cgggt
//
LOCUS       DAOQKN010000241         3077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_21123_length_3077_cov_5.418266, whole genome shotgun sequence.
ACCESSION   DAOQKN010000241 DAOQKN010000000
VERSION     DAOQKN010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21123_length_3077_cov_5.418266"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..349)
                     /locus_tag="PLJ64_11845"
     CDS             complement(110..349)
                     /locus_tag="PLJ64_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94218.1"
                     /translation="MATESEKTTVDRRPACPGCGEPWLRPTQLPGRFRCVYCLSRYEL
                     VSQCPNCGEHQTIARMSTTEDMSCQSCGASMLQPI"
     gene            complement(498..1853)
                     /locus_tag="PLJ64_11850"
     CDS             complement(498..1853)
                     /locus_tag="PLJ64_11850"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNC94219.1"
                     /translation="MSLVTPERQVAFDVLRRTFEREEHTEVAFREEAAARELTGRNRA
                     QAQRLSYGAVQRRGTSDWVISSFMKQKSRRPDPAVAAALRLGIFELLFSDGTPDHAAV
                     DQAVSLVRAAGADHAAGFANALLRRTLRERDSLTEKLADDSTPGKAAIAHSIPDWLAR
                     LWWEELGPDRARALMAASNRPSERAVRVNTARTTVEEAINRLSGPDLELVPSEGPWPL
                     APPELLVARGSLAAIEEAAQEGLVVSQTRGSAAVVEVLDPLPEERILDLCAGPGIKTG
                     QIAARVGRAGNMVSVEPDEERAHEVAAQLERLGFHHGLVVEVDARHGEIMSGFDRVLV
                     DAPCSDLGALASRPDARWRKSPALIERVAELQGEILDRAAGFLREGGSLVYSTCTISK
                     RENSDQALALAKEHGLTIDDLGGRAPQLADPHDSRFLQLMPDRDHTTGFFIARFISGG
                     D"
     gene            complement(1850..2773)
                     /locus_tag="PLJ64_11855"
     CDS             complement(1850..2773)
                     /locus_tag="PLJ64_11855"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNC94220.1"
                     /translation="MAGVFLGTSEFAGTVLDILAASDQRPELVVTLPDRRRGRGRREQ
                     ASPVADKAAALGIEVLKSGNVNEGEDLGRILEVGGGWASICAFGQLIKEPLLTELPML
                     NVHPSLLPRWRGAAPVERAIMEGDEVTGVCVMRLVAGLDSGPVAMREVTAIGQDEDFG
                     SLSARLAEIGGRLLVAALDGAGRGEIEWQEQGEDGLTYAEKITAGDREVKTSGTAREV
                     HNQVRGLTPHIGAWFALEGDDRLGIRRTAVLDEGPAAGELVAAGGWPVLGCAEGAVRL
                     ERVQPPGKKEMDGDAWLRGSGQGVPGSPGKQ"
     gene            complement(2779..>3077)
                     /locus_tag="PLJ64_11860"
     CDS             complement(2779..>3077)
                     /locus_tag="PLJ64_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNC94221.1"
                     /translation="NPAIEWLSDETEVAAEGCLSIPRVIVEVDRPLYARVRGQDRNGA
                     EVLIEASGHEARVLQHEIDHLDGILILDRTEKKQRKGAMKALRRGESYSPHDDD"
BASE COUNT          471 a         1055 c         1033 g          518 t
ORIGIN      
        1 gaagtcggcg gagaggatcg aaggagcgat gcggatgccc tggaaagctt cgggcacagg
       61 ggtttcggag ttgcggggct ggttggtcat cctgggatcc ggtcggggtt cagattggct
      121 ggagcatcga ggcgccgcag ctctggcagc tcatgtcctc ggtggtgctc attctggcga
      181 tcgtctgatg ttcaccgcag ttcgggcact gggaaaccag ttcgtagcgg gaaaggcagt
      241 acacacagcg gaaccggccg ggaagctgag tcggtcgcaa ccagggttcc ccgcagcccg
      301 gacaggccgg acggcggtcg actgttgtct tctctgactc ggtcgccatg gggcgattat
      361 cccggaccgg tcggaaactc ggcgcgcctc cccgttccgg gggcgtttgg ccaccgaaaa
      421 ggtgggtgat cgcccccgga acgggctggc acgaacgcca ccggacttcc gggggcgttc
      481 ggccacggat ccagtggtca atcgcccccg gaaatgaagc gggcgataaa gaagccggtc
      541 gtgtggtcgc gatccggcat cagctggagg aagcgggagt cgtgcgggtc cgccagctgc
      601 ggggcgcgtc caccgagatc atcgatcgtg aggccatgct ctttcgccag ggcaagggcc
      661 tgatcgctgt tttcgcgttt cgagatcgtg caggtcgagt agacgagact tccgccctcg
      721 cggagaaagc cggccgctcg atcgagaatc tcgccctgga gctccgccac cctttcgatc
      781 agggccgggg acttccgcca gcgggcatcc ggccgggaag cgagcgcgcc gaggtcggag
      841 cacggtgcgt cgaccagaac ccggtcgaag ccgctcatga tctcgccgtg cctggcgtcc
      901 acttcgacca cgagcccgtg atggaagccg aggcgctcga gctgggccgc gacctcatgg
      961 gcccgctctt catccggctc gaccgacacc atgttcccgg ctcgccccac ccgggcggcg
     1021 atctgaccgg tcttgattcc ggggcccgcg cagaggtcga ggatgcgttc ctccggcagc
     1081 gggtccagca cctcgaccac ggccgccgat ccgcgggtct gggaaacgac gagaccctcc
     1141 tgcgcggctt cctcgatcgc ggcaaggctg ccccgggcga ccaacaattc cggtggcgcg
     1201 agtggccagg gcccttcgga cggaaccagc tccaggtcgg gtccggaaag ccgattgatc
     1261 gcttcctcga ccgtggtccg ggccgtgttt acccggaccg cccgttcgga gggccggttc
     1321 gaggctgcca tcagggcgcg ggcccggtcg ggtccgagtt cctcccacca gagccgtgcc
     1381 agccagtccg ggatcgagtg ggcgatcgcc gccttcccgg gggttgaatc atcagccagt
     1441 ttctccgtca gggaatcgcg ttcgcgcagg gtgcgacgca gcagtgcgtt ggcgaatccg
     1501 gctgcgtggt cggcccccgc cgcccggacc aggctgaccg cctgatcgac ggcggcgtga
     1561 tcgggggtcc cgtcggagaa gagcaattcg aagattccca gccggagcgc ggcagcaacg
     1621 gccgggtcgg gtcgcctgga cttctgcttc atgaagctcg atatgaccca gtccgaggtg
     1681 ccgcgacgct gaaccgcgcc gtaggagagc cgctgcgcct gggcgcggtt tcggcccgtc
     1741 agttcccggg ccgcagcttc ctcccggaag gcgacttcgg tgtgttcttc ccgctcgaaa
     1801 gtccggcgga ggacgtcgaa ggcgacctgc cgctcggggg tgaccaggct cactgttttc
     1861 cggggctgcc cggcacgccc tgtccgctgc cccggagcca ggcgtcgccg tccatttcct
     1921 tcttgcccgg cggctggacc ctttccaggc gaaccgcacc ttcggcgcaa cccagcaccg
     1981 gccagccccc ggctgcgacc agttcaccgg ccgccggccc ctcatcgagg accgcagtgc
     2041 ggcggattcc gagccggtca tcgccttcga gcgcgaacca ggcaccgatg tgaggggtca
     2101 ggccgcgaac ctgattgtgg acctcgcgtg cggtcccgga agtcttcact tcacgatccc
     2161 cggcggtgat cttttcggcg taggtcaggc catcctcgcc ctgctcctgc cactcgattt
     2221 ccccgcggcc ggcaccgtcg agagccgcca ccagcagtct cccgccgatc tccgcgagcc
     2281 gggccgagag ggaaccgaaa tcttcgtcct ggccgatcgc cgtgacttcc cgcatcgcca
     2341 ccggccccga atcgaggccg gcgaccaggc gcatcacaca gaccccggtc acctcgtcgc
     2401 cctccatgat cgcccgttcg accggggccg ccccccgcca gcgcggaagc agcgaaggat
     2461 ggacgttcag catcggcaac tcggtcagca ggggctcctt gatcagctgg ccgaaggcac
     2521 agatcgacgc ccacccgcca ccgacttcga ggattcgccc gaggtcttca ccctcgttga
     2581 cgttgccgct cttcaggact tcgatgccga gggctgcggc cttgtcggcg acgggagaag
     2641 cctgttcccg gcgccccctg ccgcgacgcc ggtccggcag ggtgaccaca agctccggcc
     2701 gttggtccga ggcagccagg atgtccagca ccgttcctgc gaattcagag gtaccgagaa
     2761 agacgccggc catttgcttc agtcgtcgtc gtggggcgag tagctctcgc cccgccgcag
     2821 ggccttcatc gcgcccttgc gctgcttctt ctcggtgcga tcgaggatca ggatgccgtc
     2881 gaggtgatcg atttcgtgct gcagcacccg ggcctcgtgt ccggaagcct cgatcagcac
     2941 ttcggcgccg ttccggtcct ggccacggac cctggcatac agcgggcggt ccacctcgac
     3001 gatcacccgg ggaatgctca ggcaaccttc ggccgcaacc tcggtctcgt cggaaagcca
     3061 ttcgatggcg gggttga
//
LOCUS       DAOQKN010000242         3036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_21634_length_3036_cov_4.942301, whole genome shotgun sequence.
ACCESSION   DAOQKN010000242 DAOQKN010000000
VERSION     DAOQKN010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21634_length_3036_cov_4.942301"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..820)
                     /locus_tag="PLJ64_11865"
     CDS             complement(17..820)
                     /locus_tag="PLJ64_11865"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HNC94222.1"
                     /translation="MSRPTALLFTHSHAAGMDRAVEVAHAAAERHGWELLASKDAEGS
                     EPSLFIVLGGDGTILHALRSSLDRRVPVFGINFGRVGFLAGVEADQIELGLDRAFAGE
                     YEILDLPGLAISANGETQIALNDASFSRRPRGGVAELSYRIAGEEVGHVRCDGLVAST
                     ATGSTGYNLANHGPILAWGVAGYVVSYIAPHTLTARALVVAPGDVLHVMNHRSRDGVD
                     IAVDGVQIGELKPSAEAEVSYLPGLARLAQLPGSNFYHRIREKFGHLAV"
     gene            complement(817..1659)
                     /locus_tag="PLJ64_11870"
     CDS             complement(817..1659)
                     /locus_tag="PLJ64_11870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HNC94223.1"
                     /translation="MAKRSRLDLLLAERGTFTSRTAASRAIRAGQVKLGKDGPIALRP
                     SQEVPLDQEIEVLEGQRYVSRGGVKLENGLDALGIDVTGKLCLDVGASTGGFTDCLVQ
                     RGARHVIALDVAYGQIDASLRDRTEITVIERLNARDLLPADLEYRPELTVIDVSFISL
                     TKVLPAVVNTVAEDSTILAMVKPQFELGRARVGRGGVVRNQADRREAVEAVADFALEL
                     GLSLDGIAPTGLPGPKGNLETFVQLGHSKASGSGGESTAGEGSSAGARGADSGAALCE
                     GVEL"
     gene            1926..2279
                     /locus_tag="PLJ64_11875"
     CDS             1926..2279
                     /locus_tag="PLJ64_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94224.1"
                     /translation="MTARPIELSVHNAVVLATAPLLMIVPYLLTFSPGIGYLTFFLGA
                     SLMGVSLAGSSPQRPLPLSAQSGFDSAIGIAIFAIGILAGITGQAAMTTIFLVGFGAA
                     HLALTASTRYSARGA"
     gene            2593..>3036
                     /locus_tag="PLJ64_11880"
     CDS             2593..>3036
                     /locus_tag="PLJ64_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94225.1"
                     /translation="MTPATLTSADFEVEAFGLIAELEGEADKGSRQTAIDHRLEALRE
                     SWRQLETEDQERCRTLTSRIAALVAARPGIDTGQPASPRRPQTPAEAAGQTVLIADEQ
                     EEAALALQGLEGIELLESVPTRLYEGPRDPEGLLAHMDYDSFRPGQ"
BASE COUNT          512 a         1035 c          958 g          531 t
ORIGIN      
        1 ttaagcagag cgcgcgctaa acagcgaggt ggccgaactt ctccctgatc cggtggtaga
       61 agttcgatcc cggcagctgg gccagacggg cgaggccggg caggtaactg acctcggctt
      121 cagccgaggg tttgagctcg ccgatctgga ccccgtcgac cgcaatgtcg acgccgtccc
      181 ggctgcggtg gttcatcaca tggagcacgt cgccgggcgc cacgacaagc gcccgggcgg
      241 tcagggtgtg cggggcgatg tagctcacca cgtaaccggc cacgccccag gccaggatcg
      301 gcccgtggtt ggcgaggttg tatccggtgg agccggtcgc ggtcgaggcg accaggccgt
      361 cgcagcggac gtgccccacc tcttccccgg cgatccggta actgagttcg gccactccgc
      421 cgcgcggccg ccggctgaag ctcgcgtcgt tcagcgcgat ctgcgtttcg ccgttggccg
      481 atatcgccag tcccggcagg tcgaggatct cgtattcgcc ggcgaaggcc cggtcgagtc
      541 cgagttcgat ctggtcggcc tcgaccccgg cgaggaatcc gacccggccg aagttgatcc
      601 cgaagacagg aacccgacgg tcgagagatg aacgcagggc gtgaaggatg gttccgtcgc
      661 caccaagcac gatgaacagg gaaggctcag atccctcggc gtccttgctc gccaggagtt
      721 cccagccgtg ccgttcggcg gcggcgtggg caacctcgac cgcccggtcc atgccggcgg
      781 cgtgcgagtg ggtgaagagc agggcggtgg ggcgactcac agttcgacac cttcgcagag
      841 agcggcgccg ctgtcagccc ctcgggcccc ggcggatgat ccttccccgg ccgtcgattc
      901 gcccccggac ccgcttgcct tcgaatgccc gagctggacg aacgtttcga gattgccctt
      961 gggcccgggc aatccggtcg gcgcgatccc gtccaggctc agcccgagct cgagcgcgaa
     1021 gtcggcgacg gcctccaccg cctcccgccg gtccgcctga tttcgcacca ctccgccccg
     1081 gccaaccctc gcccggccca gttcgaactg gggtttgacc atggccagga tcgtcgaatc
     1141 ctcagccacc gtgtttacga ccgccggcaa caccttggtc agagagatga acgagacgtc
     1201 gatcacggtc agctcggggc ggtattcgag gtcggccggg agcaggtccc gggcgttcaa
     1261 cctctcgatc accgtgattt cagtccggtc gcgcaggctg gcgtcgatct gaccgtaggc
     1321 gacgtcgagc gcaatcacat gcctggcgcc acgctggacg aggcagtcgg tgaagcctcc
     1381 ggtcgaggcg ccgacgtcga ggcagagctt tccggtgacg tcgatcccga gcgcgtcgag
     1441 cccgttctcg agcttgaccc cgccccgcga gacgtatcgc tggccttcga ggacctcgat
     1501 ctcctggtcc agggggacct cctggctcgg cctgagtgcg atcggcccgt ccttgccgag
     1561 cttgacctgc ccggcccgga tcgcccggga cgccgcggtc cgggaggtga aggtgccccg
     1621 ctcggcaagg agcaggtcaa gtcgggatcg cttggccatc tgaccagaac cgtaccggga
     1681 ccgaaaacaa accccgaccc gccccctccc ctccggtggg tcggatttcc cgtaccagcg
     1741 acggaaatcc gacccaccgg cttcgggact ctggctgaag gtgaatttct ggctgcctgt
     1801 aacggaaaat ccggctttct gaacccgaaa accgtgaatt cgacatgaat tcatgtcaat
     1861 gtgtgatccg actcacacac gaaaagtaag tggtcgtcta ctttgctctc aggagaacga
     1921 tgaacatgac cgcgagaccc atagaactca gcgtccacaa cgcagtcgta ctggccactg
     1981 cgccccttct gatgatcgtt ccgtacctgc tgaccttcag ccccgggatc ggttatctga
     2041 ccttcttcct cggcgcttcg cttatgggag tttcccttgc ggggtcgagt ccgcagcgtc
     2101 cgcttccgct ttccgcccag tccggtttcg actcggcgat cggtatcgca atcttcgcaa
     2161 tcggcatcct ggccggcatc accggacagg cagccatgac cacaatcttc ctggtcgggt
     2221 tcggggccgc tcacttggca cttaccgctt caacgcggta tagcgcccgc ggcgcataaa
     2281 gagcccacct acctcccatc gatcaaaaaa cacataagag atcattgaat ctctctcctc
     2341 cctaagcaac accaagagcc ccgctttccc ggcggggctc ttttcttgca ggacgcgaaa
     2401 ggtcggtggg tcggatttcc gttcccccgg cgggttttcc ggcccacccc ggtgctggcg
     2461 accggcccgg cttcccgggt gggtcggatt tccgtcgccg gtgctggaaa tccgacccac
     2521 cggtcgaacg gtggcgacgg gctggtgcgg gggcgagttt tcggcgtcag atgctgtctt
     2581 tagtctggtc cggtgactcc agccaccctg acatccgccg atttcgaggt cgaggcattc
     2641 ggcctgatcg ccgagctcga gggcgaagcg gacaaaggat cccgacagac cgcgatcgac
     2701 caccgcctcg aagctcttcg cgagtcgtgg cggcagctcg aaaccgaaga tcaggaacgg
     2761 tgcaggacgc tgacctcgcg gatcgctgcc ctggtggcag cccgaccggg aattgacact
     2821 ggccagcccg cgtccccgcg ccggccgcaa accccggccg aagccgccgg ccagaccgtg
     2881 ctgatcgcgg atgagcagga ggaggccgcg ctcgccctgc agggcctcga agggatcgag
     2941 cttctcgaat cggtcccgac ccgcctttac gagggccccc gtgatcccga gggcctgctc
     3001 gcccacatgg attacgacag tttccgcccc ggccag
//
LOCUS       DAOQKN010000243         3020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_21830_length_3020_cov_6.040135, whole genome shotgun sequence.
ACCESSION   DAOQKN010000243 DAOQKN010000000
VERSION     DAOQKN010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21830_length_3020_cov_6.040135"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="PLJ64_11885"
     CDS             complement(<1..837)
                     /locus_tag="PLJ64_11885"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HNC94226.1"
                     /translation="MICLVGFMGAGKSTALVELAAHGLHSVDADSLIEERAGRSITQI
                     FEADGEAAFRDLEREVVIEALADPSIDVLALGGGAVESEAIREALVVDGHTVVWLEIS
                     AESAWRRVRNSKRPLARDESAFRALFAARQPLYDSVSDAILPGVARRVWDRAYEAVRG
                     LEDLPEGTRMFWAESSGGEYPVYVGRELLGSALFEAGSPALPGSGRTFCFTDESVGGI
                     YGERLGELAGTVTVPPGESAKSMVQLDESLREMARAGVTRSDRLIALGGGVVGDLAGF
                     AAH"
     gene            complement(834..1994)
                     /gene="aroC"
                     /locus_tag="PLJ64_11890"
     CDS             complement(834..1994)
                     /gene="aroC"
                     /locus_tag="PLJ64_11890"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNC94227.1"
                     /translation="MSFRFTTAGESHGPGLVTIVEGLPAGLELDRETLDSDMARRQLG
                     HGRGGRMKIETDQVEIRAGVRHGFTMGGPVAALVANRDYKNWDERMNPWPIEGEIPPV
                     TMPRPGHADLVGMWKYKHDDLRPILERASARETAARVAAGAIARAFLTAVGVTVRSHV
                     TQIGSVVAPERDDLKIDDFEGVDDDPVRCLDSETSAAMVEEINVLRKKNESLGGTFEV
                     RVFGLVPGLGGHISWEDRLDGRLAQAICSIQSIKGVSIGQAWDVAGRPGSAAHDEIFY
                     SEERGYYRETNHAGGLEGGMTNGMPLSVRAAIKPISTMTKPLRSVDTATKEPAQAHKE
                     RTDSTVVPAAAVVGEAMICLALVKTYREKFGGDHMDDVLAAIDAYKERIGWR"
     gene            2093..2572
                     /locus_tag="PLJ64_11895"
     CDS             2093..2572
                     /locus_tag="PLJ64_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94228.1"
                     /translation="MLILLGISSVIAFVVPEPQRNDPATDQAPVTGTTGSTGVPAESG
                     ATGKAGTTGEDGTGKRKGEPKASEPGAARSGGVPIETVTLGGAKPARIEAKAGTRMII
                     TVRSGEGSDVEIEDLGLAGFADPYAPAVFDVILPPEPGRYPIGAPGEKPSAVIIASS"
     gene            complement(2778..>3020)
                     /locus_tag="PLJ64_11900"
     CDS             complement(2778..>3020)
                     /locus_tag="PLJ64_11900"
                     /inference="COORDINATES: protein motif:HMM:NF036850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNC94229.1"
                     /translation="LGGGDGLADLPLDPGGFHPGQTVVDMVYGETPGTLIATAMQDGA
                     KTVDGLEILVRQGARSFEIWTGKRPDLESMRQAART"
BASE COUNT          469 a         1013 c         1028 g          510 t
ORIGIN      
        1 gtgggcggcg aacccggcca gatccccgac tacccctccg cccagggcga tcagccggtc
       61 cgagcgggtc accccggcgc gcgccatttc gcgcagggac tcgtcgagct gaaccattga
      121 cttggccgat tccccgggag gtaccgtgac cgtgccggcg agctcgccga gtcgctcgcc
      181 gtagatcccg ccgaccgact cgtcggtgaa gcagaaggtc cggcccgatc ccggcaacgc
      241 cggcgaaccg gcttcaaaga gcgccgatcc gagcagctcc cggccgacgt aaaccgggta
      301 ctcgccgccg gagctttcgg cccagaacat ccgggtgcct tcaggcagat cttccaggcc
      361 ccgtacggcc tcgtaggccc ggtcccagac ccgccgggcg acaccgggca ggatcgcgtc
      421 cgaaaccgag tcgtaaaggg gttgacgcgc cgcgaaaaga gcgcggaatg cggattcgtc
      481 acgggccagc gggcgcttcg agttgcgaac ccggcgccag gccgattcgg cactgatctc
      541 cagccagacg accgtgtggc cgtcgaccac cagggcctcg cggatcgcct ccgattccac
      601 cgcgccaccg ccgagcgcca gcacgtcgat cgagggatcg gccagcgcct cgatcacgac
      661 ttccctttcc aggtcgcgga aggcggcttc gccgtcggct tcgaagatct gcgtgatcga
      721 ccggcccgcc cgctcctcga tcagggagtc ggcgtctacg ctgtggaggc cgtgcgcggc
      781 gagttcgacc agggccgtcg acttgccggc gcccatgaac ccgaccaggc agatcatcgc
      841 cagccgattc gctctttgta ggcgtcgatc gcggccagca cgtcatccat gtggtcgccg
      901 ccgaacttct cgcggtaggt cttgaccagg gccaggcaga tcattgcctc gccgaccacc
      961 gcggcggccg ggaccaccgt cgagtcggtc cgctccttgt gggcctgggc cggttccttg
     1021 gtggctgtgt caaccgagcg gagcggcttg gtcatggtcg agatcggctt gatcgcggcc
     1081 cggaccgaga gcggcatgcc gttggtcatg ccgccttcga ggccgccggc atggttggtt
     1141 tcgcggtagt agccgcgctc ctccgagtag aagatctcgt cgtgggcggc ggagccgggc
     1201 cggccggcga cgtcccaggc ctggccgatc gaaacgccct tgatcgactg gatcgagcag
     1261 atcgcctggg cgaggcggcc gtcgaggcgg tcctcccagg agatgtgacc gccaagaccg
     1321 gggaccagcc cgaaaacacg cacctcgaag gtgccgccga ggctttcgtt cttcttccgc
     1381 aggacgttga tctcctcgac catcgcggcc gaggtttcgg agtcgaggca acgaaccgga
     1441 tcgtcgtcga ccccttcgaa atcgtcgatc ttcaagtcgt cccgttcggg ggcgaccacg
     1501 gaaccgatct gcgtgacgtg gctgcgcacg gtgaccccga cagcggtcaa aaaggcccgg
     1561 gcgatcgcgc cggcggcaac ccgggcggcc gtctcgcggg cgctggcccg ttcgaggatc
     1621 ggccggaggt catcgtgctt gtacttccac atcccgacca ggtcggcgtg accgggccgc
     1681 ggcatggtga ccggcgggat ctcgccttcg atgggccacg ggttcatccg ctcgtcccag
     1741 ttcttgtaat cgcggttcgc gacgagggcg gcgaccggcc cgcccatcgt gaacccgtgc
     1801 cgcacgccgg cccggatttc aacctggtcg gtttcgatct tcatgcggcc gccgcggccg
     1861 tggccgagct ggcggcgcgc catgtcgctg tcaagcgtct cgcggtccag ttcgagcccg
     1921 gcgggcaggc cttcgacgat ggttacgagg ccgggtccgt gggactcgcc tgcggttgtg
     1981 aatcggaaac tcattgaggc ttctaggcta ttgacaactg catgcccgac gctgtctcaa
     2041 ccaacccggg gtccgacccc tgcatatggc tgcccgtcga ctgctgatcg taatgctgat
     2101 cctgctgggg atctcgtcgg tgatcgcttt cgttgtcccg gagccgcagc gcaacgatcc
     2161 ggcgaccgat caggcgccag ttaccggaac cacaggctca acgggcgtcc cggccgaaag
     2221 cggggcgacg ggcaaagccg gcacgaccgg tgaagacggc acgggcaagc gcaaagggga
     2281 gccgaaagca tccgagccgg gggccgccag gtccggcgga gtcccaatcg aaacggtgac
     2341 tctcggtggc gcgaagccgg ccaggattga ggcgaaagcc gggacgcgga tgatcatcac
     2401 ggtcaggtcc ggcgagggtt cggacgtcga gatcgaggat cttggcctgg ccgggttcgc
     2461 cgacccctac gcgccggcgg tctttgacgt gatcctcccg cccgaacccg gcaggtaccc
     2521 gatcggcgcc ccgggcgaga agcccagcgc ggtaatcatc gccagctcct gagccaaatc
     2581 tccggtgggt cggatttccg tcatgtgtgc tggtaatccg acccacctgg tgctggcggg
     2641 ctggtcggtt ccccgggtgg gtcggatttc cgtcatgtgt gctggtaatc cgacccacct
     2701 ggtgctggcg ggctggtcgg ttgcccgggg tgggtcggat ttacgtcgtg tgggcgggaa
     2761 atccgaccca ctggaagtca ggttcgggct gcctggcgca ttgattcgag gtccggtctt
     2821 tttccggtcc agatttcgaa ggaccgggcc ccctggcgga ccaggatttc gagcccgtcg
     2881 acggtcttag ctccgtcctg catggcggtg gcgatcaatg ttcccggggt ttcgccgtaa
     2941 accatgtcga cgacggtctg gcccgggtgg aacccgcccg gatcaagcgg aaggtcggcc
     3001 aggccgtccc ctccccccag
//
LOCUS       DAOQKN010000244         3004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_22040_length_3004_cov_6.211936, whole genome shotgun sequence.
ACCESSION   DAOQKN010000244 DAOQKN010000000
VERSION     DAOQKN010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22040_length_3004_cov_6.211936"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(235..2121)
                     /locus_tag="PLJ64_11905"
     CDS             complement(235..2121)
                     /locus_tag="PLJ64_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94230.1"
                     /translation="MRRELPITMHDPVLDEALKRLAAEASTRFTSLVATGDEIPFDVA
                     ENNGDSSHFYRYVPLTSRFISTHLEELKSLPSYGPARGAIASSDVAAPYLESRGLPVP
                     SEPTRRAEEMIAVFITSLWEGTTEFSLDISRVEAALAGLEVQTRDIREADVLIVPLVG
                     FEMTPTEVTLEGGIRIAQADSIDAPLEATSSEGTGRSAWQTAYFAMTELESTEDGPRQ
                     AVNRLNGMIRALRLYKEGSVGLGPFAFAPVGESSWRRIETAVSPPRPGSYFLIESEVD
                     SLNELIRHLAKESPAADRMEFAQKRFQYGCERENPVEALSDHLLAIRASLGGEGVIDA
                     PLAARAAALITGNADDLRSRERIELALDLEQSLVNGEDLMSIGGESATYIAAWVEDST
                     RHILREAVLGHYGMNLNIAAEETLITSGLTAGEGSARDYGSSAEWDPVEEPAEDRTTE
                     PVAEEGADIHIFKPRVVGGTSVDGPVDDPLFGIVPEFQAEPLPEAPLQPMPEIERALG
                     IESSVEPSGEPEFVVSSRGNGKGLFDQGTGDEDSGTTRLLEPIPEAGEISVTASTEIG
                     ATLREDWLSQAHSNGSTMEWPSHAMDGLDEARHNPHPKPNRNFFPDPGTTEWSISEMR
                     FKNR"
     gene            2265..2987
                     /locus_tag="PLJ64_11910"
     CDS             2265..2987
                     /locus_tag="PLJ64_11910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94231.1"
                     /translation="MSAAVLAPTAAARPGDLDPDFGVGGKTVITPASGGMEIPADMAM
                     TPDGGYVVAGADRHAGKSLVVRFDADGNLNTDFGDQGELRTAGNGWQQVEVTADGHIV
                     LAGGNNSQMVFERLNADGTPDTGFGGDGGVALDVSSLTLPDGATRKAAVSAMRILGDG
                     SIRAIGTGFDCTGEWTDSSCPEALLLGVDQDGSLAAGLGTGGVKDLGIRGWLDAASIF
                     ADGSALVIRSHYCDPQYGGGSQ"
BASE COUNT          509 a          942 c         1016 g          537 t
ORIGIN      
        1 ggcctctgcc agatcaacga gtacctcttc gtcgaagagg atgaacgagt cgaaagagca
       61 atctcctgac ggtggttacg ccgcggcgtc gggtgagcat cgccgggaca cgacacgcgc
      121 ggcattcagc cgcgctgtca ttgagcgtcc cggacgacgc tcacccgacc ccacggctac
      181 atcaacccgg agcacaccca caccaggcct ttggaccagg ctggtgaacc cgggttagcg
      241 attcttgaag cgcatttccg agattgacca ttcggtcgtt cccggatcgg ggaaaaagtt
      301 gcggttcggc ttgggatgcg ggttgtggcg ggcctcgtca agaccgtcca tcgcatgcga
      361 aggccattcc atggtcgagc cgttgctgtg cgcctgggag agccagtcct cgcgcagggt
      421 cgcgccgatc tcggtcgagg cggtcaccga gatttcgccc gcctccggga tcggttcgag
      481 cagccgggtg gtgccggagt cttcgtcgcc cgtgccctgg tcgaagagac ccttgccgtt
      541 gccgcgagat gacacgacga actcgggctc gccggagggc tccacactgg attcgatgcc
      601 gagcgcacgc tcgatctccg gcatcggctg aagcggggct tcgggcagcg gctcggcctg
      661 gaactcgggc acgatgccga agagcgggtc gtcaaccggg ccatcgacac tcgttccgcc
      721 aaccacgcgc ggcttgaaga tgtggatgtc ggcgccttcc tcggccaccg gctccgtggt
      781 ccggtcttcg gccggttcct cgactggatc ccattcggcc gaagatccgt agtcgcgggc
      841 gctgccttcg ccggcggtca ggccgctggt gatcagggtt tcctcggcgg cgatgttgag
      901 gttcatgccg taatggccaa gcaccgcctc acgcaggatg tgccgggtcg aatcctcgac
      961 ccaggcggca atgtaggtgg ccgactcgcc accgattgac atcaggtctt ccccgttgac
     1021 cagcgattgt tcgaggtcga gcgcgagttc gatccgttcg cgcgagcgca ggtcatcggc
     1081 gttgccggtg atcagcgctg ccgcccgggc ggccagggga gcgtcgatca cgccctcccc
     1141 gccgagcgag gcacggatcg ccagcagatg atcactcagg gcctcaaccg ggttctcccg
     1201 ctcgcagccg tactggaagc gcttctgcgc aaattccatg cggtcggcgg ccggcgactc
     1261 cttggccagg tgccggataa gttcgttcag ggaatcgact tccgattcga tcaggaagta
     1321 gctgccgggc cgcggcggcg agaccgcggt ctcgatccgg cgccagctcg actcgcccac
     1381 cggcgcgaag gcgaagggcc ctaggccgac cgagccttcc ttgtagaggc gaagcgcgcg
     1441 gatcatgccg ttcagccggt tcacggcctg gcgcggcccg tcttcggtgc tttcgagttc
     1501 ggtcatcgcg aagtaggcgg tctgccaggc gctgcggccg gtgccttcgc tggaggttgc
     1561 ttcaagcggc gcgtcgatcg aatcggcctg ggcgatccgg atcccgccct cgagggtgac
     1621 ctcggtcggg gtcatctcga aaccgaccag cggcacgatc agcacgtccg cctcccgaat
     1681 gtcacgggtc tggacttcaa gcccggccag agcggcttcg acccggctga tgtcgagcga
     1741 aaattcggtt gtgccttccc agagcgaggt gatgaagacg gcgatcatct cttcggcccg
     1801 gcgggtcggc tcggaaggaa ccggcagtcc ccgggactcg agatagggag cggcgacgtc
     1861 ggaggaggcg atcgccccgc gcgcgggacc gtatgaagga agcgacttca gctcttcgag
     1921 gtgggtcgag atgaagcgtg aagtgagcgg cacgtaccgg tagaagtgag agctgtcccc
     1981 gttgttctcg gccacgtcga aggggatctc gtcaccggtg gccaccaggg aggtgaaccg
     2041 cgtcgatgcc tcggctgcga gccgcttcaa agcctcatca agaacaggat cgtgcatggt
     2101 gattgggagt tcccttctca tcagaggcat cctgcgaatg caggattaac tgcaactcac
     2161 cccctcgaat acggttatgg gagcgtcaag ccgttgtccc ggtcatccaa ggaggggtct
     2221 tgttcaaatt cgcgcgtctt tcaggcttgg tcgcgttcct gaccttgtcg gctgcggttc
     2281 tcgcacccac ggcggccgcc cgcccgggtg atctcgaccc cgatttcggt gttggcggca
     2341 aaacggtcat cacgcctgct tccggtggca tggagatccc ggcagacatg gccatgaccc
     2401 ccgacggggg ttatgtggtg gcgggggccg accgccacgc aggcaaatcg ctggtcgtca
     2461 ggttcgacgc ggacgggaat ctcaacacgg acttcggcga ccagggtgaa ctcaggacag
     2521 ctggcaacgg ctggcaacag gttgaggtaa cggccgacgg ccacatcgtc ctggccggtg
     2581 gcaacaactc gcaaatggtc ttcgagcgcc tgaatgctga tggcaccccc gacaccggct
     2641 tcggcggaga tggcggggta gccctggatg tttcgagcct gaccctgccc gacggggcaa
     2701 ccaggaaggc agccgtatcg gcaatgagga ttctcggcga cgggagcatc agggcgatag
     2761 gcacgggctt cgactgcacc ggtgaatgga ccgattcgtc ctgtcccgag gctttgctgc
     2821 tcggagtcga ccaggacgga tcgcttgccg cgggcctcgg aacgggcggc gtcaaggacc
     2881 tcggcatcag aggctggctc gacgccgcct cgatcttcgc ggacggcagc gccttggtca
     2941 tccgcagcca ctactgcgac ccccagtacg gcggggggtc gcagtagtgg ctgcggatga
     3001 ccaa
//
LOCUS       DAOQKN010000245         3004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_22045_length_3004_cov_4.166497, whole genome shotgun sequence.
ACCESSION   DAOQKN010000245 DAOQKN010000000
VERSION     DAOQKN010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22045_length_3004_cov_4.166497"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="PLJ64_11915"
     CDS             <1..647
                     /locus_tag="PLJ64_11915"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HNC94232.1"
                     /translation="STGVSVGNGGAGITASNSGITGGGTGFSRLNVFLSIARAEKGYD
                     LDGGFDSDDNEACISARHTSALSLRPNICGIAEDNPIRELPGFIDPVSDFHLAAGSPL
                     IDAVKNFSYPIPSVDLEGNPRDATDPDPGAYEFQKPAPPRRCVVPRLRGLKMPQVRNR
                     LRNSNCALGRVTRKKVAAKRKAGRVLRQSPRAGLTLNEGARVRVTVGRKARSRR"
     gene            644..2557
                     /locus_tag="PLJ64_11920"
     CDS             644..2557
                     /locus_tag="PLJ64_11920"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNC94233.1"
                     /translation="MSKAWSSSIRPMPEAGEHVGGYSVVGEIGRGTTGLILEVSAGSD
                     DPLAMKLLAAGLAADPATRERFERGIRAQREVAGSGVVPVLDAGDDPERGPWLTMPLF
                     TGGNLADRIREGGLTPAGLVEILEPVAEGLDRAHQDGVVHRDVKPSNVLIDPDGRGSL
                     ADFGLARGEGDQSQTLAGTVVGTLACVSPEVVQGNAATAASDRYGFAAILVEGLTGQS
                     VFPRSSDAAVLYAHAQLPPPSVSARRDSLPAGLDGIFERGLSKDPGQRPATCSELLVQ
                     VRSAFGPDLDSAPALESSRLPATDDRTLEPEAGSGDGPGDRGRRPRAAGVLAGFGAIL
                     LAGLLVFAILRNGDSGSTSEAAPPVSDGLVALGSDLPSEGVESVDCRGREAGPNSPSC
                     SVLQTALPGSRLLVPSKGAVHAWSVRGAAGELALQVIRRRDGQYFQLFRSQVTLVPDH
                     NVHTFPVELAVEAGDMVALAVSPDTDIGVIPDVPGARTDRFFGPIGPETRPDLGPGTG
                     FDSEVLLRVEYEPGGVPEPPEQIFGAEAAGLADGKVVASSDTSLPDGRKVEVHLTEFE
                     DRVWVDLYLGAVRQSRIEVPDLQAGGEVVEFKAFEAPGSPSQLNVGWINPGMERPIEH
                     YFGLSGSALEFYS"
     gene            complement(2576..>3004)
                     /locus_tag="PLJ64_11925"
     CDS             complement(2576..>3004)
                     /locus_tag="PLJ64_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94234.1"
                     /translation="SDDGLSRNGTFIGPDRVNGRRVLAHGDVIRAGQTSLVYRAPSEG
                     LDETEAASSGASAARLTPGERRVLVALCEPLLRDPGSGIAASNNEIAERLSLSVAGVK
                     TQIRALFARLEVDDLPQNRKRAELARRAVAAGLVTERDLG"
BASE COUNT          480 a         1010 c         1034 g          480 t
ORIGIN      
        1 gttcgaccgg agtttcggtg ggcaacggcg gagccggaat caccgcttcg aacagcggca
       61 ttaccggcgg cggaaccggc ttcagccggc tcaacgtctt cctctcgatc gcccgggccg
      121 agaagggata cgacctcgat ggcggattcg actccgatga caacgaagcc tgcatctcgg
      181 cccgacacac atccgcgctc agcctgcggc cgaatatctg cgggatcgcg gaagacaacc
      241 cgatccggga actgcccggc ttcatcgatc cggtctccga cttccatctc gccgcgggtt
      301 cacccctgat cgatgccgtg aagaacttca gttacccgat cccttcggtt gatctcgaag
      361 gaaacccgcg ggatgccacc gaccccgatc ccggcgccta cgaattccag aagccggccc
      421 cgccccgccg ctgcgtggtg ccgcggctgc gaggactgaa gatgccgcag gtcagaaaca
      481 ggttgcggaa ctccaactgc gcgctcgggc gggtcaccag gaagaaagtg gcggcgaaac
      541 ggaaagccgg gcgagtgctg cgtcagtcac cgcgggccgg gctgaccctt aatgaaggag
      601 cgagggtccg ggtgacggtt ggccggaagg cgagaagccg gcggtgagca aggcctggtc
      661 gtctagcatc cggccgatgc cggaggcagg cgagcatgta gggggttact cggtggtcgg
      721 ggagatcggc cgcggtacca ccggcctgat tctcgaggtc agtgccggct cggacgatcc
      781 gctggcgatg aaattgctgg ctgccggact ggcggcagac ccggcgaccc gggaacggtt
      841 cgagcgaggc atccgggcgc agcgcgaagt ggccgggtcc ggagtagtcc cggtgctcga
      901 cgcgggcgat gacccggaac ggggcccctg gctgaccatg ccgctcttta cgggaggcaa
      961 ccttgccgac cggattcgcg aaggaggcct gactcccgcc ggactcgtcg aaatcctcga
     1021 acctgtagcc gaaggcctcg accgggccca tcaagacggt gtggtccacc gggacgtgaa
     1081 gccttcgaac gtgctgatcg acccggatgg aaggggttcc ctcgccgatt tcggcctggc
     1141 ccgcggcgaa ggcgaccaga gtcagaccct cgccggaacc gtggtcggaa cgctcgcctg
     1201 tgtctccccc gaggtggttc aaggcaatgc ggcaaccgcc gcctcggacc ggtacggatt
     1261 cgcggcgatc ctggtcgaag ggttgaccgg gcagagcgtc ttccccaggt ccagcgacgc
     1321 ggccgtgctc tacgcccacg cccagcttcc cccgcccagc gtttccgcgc ggcgcgattc
     1381 ccttcccgcc ggactggacg ggatcttcga gaggggtctc tccaaggacc cgggccagcg
     1441 ccccgccacc tgctcggagc tcctggttca ggtccgctct gccttcgggc ccgacctcga
     1501 ctcggctccg gcgctcgagt cttcccggct tccggcgacc gacgacagga cccttgaacc
     1561 ggaggccgga agcggcgacg gtcccggaga ccgcggtcgc cggccgcgag cggccggagt
     1621 cctggccgga ttcggtgcga tcctgctggc aggcctgctc gtcttcgcga tcctgcgaaa
     1681 cggtgactcc ggctcgacct cggaggccgc tccgccggtt tccgatggcc tggtcgcgct
     1741 tggcagcgat cttccgtccg aaggggtgga gagcgtcgat tgccgcggcc gggaagcggg
     1801 gccgaactca ccttcctgct cggtcctcca gaccgccctg ccggggtcgc ggctgctggt
     1861 accgagcaaa ggggcggttc acgcctggag cgtccgcggc gccgccggcg agttggcact
     1921 tcaggtaatc aggcgacgcg acggccagta tttccagctg ttccgctcgc aggtgaccct
     1981 ggtccccgac cacaacgtcc acacttttcc ggtcgagctc gcggtcgagg ccggcgacat
     2041 ggtcgcgctg gccgtttctc ccgacaccga catcggcgtc atccccgatg tgcccggcgc
     2101 cagaacggac cggttcttcg gtccgatcgg accggaaacg aggccggatc tcggacccgg
     2161 caccggattc gacagcgagg tcctgctgcg ggtcgagtac gaaccgggag gagtgccgga
     2221 accgccagag cagatcttcg gggccgaggc cgccggactg gcggacggaa aggtcgtcgc
     2281 ctcctcggac acgtccctgc ccgacgggcg caaggtcgag gtacacctga ccgagttcga
     2341 ggaccgggtc tgggttgatc tctacctggg ggcggtccgc cagtcacgaa tcgaggtccc
     2401 cgatcttcag gccggtggcg aggtggtcga gttcaaggct ttcgaagcgc cagggtcacc
     2461 gagccagctc aacgtcggct ggatcaaccc gggaatggaa cggccgatcg agcattactt
     2521 cggcctctcc ggcagcgcgc tcgagttcta ctcctgaggt ggcgcggccc gcgacctagc
     2581 ccaggtcacg ttcggtgacc agaccggcgg ccaccgcccg gcgggcaagt tccgcccgtt
     2641 tgcggttctg tggcaggtca tcgacctcga ggcgggcgaa cagggctctg atctgggtct
     2701 tcactcccgc cacggagaga ctcaggcgtt cggcgatctc gttgttggag gcggcgattc
     2761 cactgcccgg gtctctgagc agtggctcgc agagcgcgac cagaacccgc cgctctcccg
     2821 gggtcagccg ggctgcgctg gccccggagg aggctgcttc ggtttcgtcg agaccctcgg
     2881 acggagcgcg gtaaacgagc gaggtctggc cggcgcggat cacgtcacca tgcgccagca
     2941 cccggcggcc gttcacccga tcgggaccga tgaaggttcc gttgcgggaa agcccgtcgt
     3001 cgct
//
LOCUS       DAOQKN010000246         2988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_22241_length_2988_cov_5.800546, whole genome shotgun sequence.
ACCESSION   DAOQKN010000246 DAOQKN010000000
VERSION     DAOQKN010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22241_length_2988_cov_5.800546"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..781
                     /locus_tag="PLJ64_11930"
     CDS             155..781
                     /locus_tag="PLJ64_11930"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNC94235.1"
                     /translation="MKVVATSVNAGDAARQALESCISKGGVALFPADGLYGLACDPLN
                     EKAIRRIHEIKGRDDGKPSAVMYLSPLAMRELIRDLGQRVSEAAARLLPGPVTLVVPN
                     PRHRYPLACRDDNSRLGIRVIDGPLSGVRFPLFQTSANLSGEPAPSVFEGVVPEVREM
                     VDLAIDGGRLTGLPSTVVDLTDYDEHGEWEILREGGMSRGDLIAALGR"
     gene            complement(855..1676)
                     /locus_tag="PLJ64_11935"
     CDS             complement(855..1676)
                     /locus_tag="PLJ64_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94236.1"
                     /translation="MRRKSMAAFSGVLLAFSFAAIVGTGSPARGDFSPDKPNGVTDPC
                     RTPKTAKKLLCPDLRIGKPSEMYLDRDLAPGQALLRSTNDIRSRGMGPMEVRGHRDRP
                     RTMNVTQAIRRRGGGYTFFPTQARLRYFNVGYQWGGAYWKIRNPLRFELWRVNAAGKK
                     IELVRTGPKQFYCFRDLVQTNPASTRSPDSAVYPACNQNPRIRHVTLGTSVGWSDIYP
                     STYDRQWINVTGLRGCFAYVLHLDPGNVLFELSKKNNYAQRIVRLPWRGYDRRGC"
     gene            1731..2177
                     /gene="rpiB"
                     /locus_tag="PLJ64_11940"
     CDS             1731..2177
                     /gene="rpiB"
                     /locus_tag="PLJ64_11940"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HNC94237.1"
                     /translation="MKIAMGSDHAGFELKEAVKAQLEDAGHEVEDVGTNSSESTDYPP
                     FAAKAAELVARGEAEKGVIVCGSGVGVSIVANKVDGIRAVNAHDAAEAEMSRRHNDAN
                     VVTLSGARLGPEDAKPIVETFLATDFDGGRHARRVGEIAEVERGEL"
     gene            2376..>2988
                     /locus_tag="PLJ64_11945"
     CDS             2376..>2988
                     /locus_tag="PLJ64_11945"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936093.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNC94238.1"
                     /translation="MSTVSEELLGASLSEVDPAVAEAIGGELNRQRNTLEMIASENFV
                     PRAVLEAAGSVLTNKYAEGYPGRRYYGGCEQVDIVEQLAIDRAMELFGAGHANVQPHS
                     GAQANNAAYMALLEPGETIMGLALDHGGHLSHGMKLNVSGKLYNVVPYHVSREDLRVD
                     MDEVAKLAEETKPSLIVAGWSAYPRQLDFAAFREIADSVGAKLM"
BASE COUNT          508 a          950 c         1025 g          505 t
ORIGIN      
        1 gatgccggct ggccggaaac cgaaatccgc ctcgatctgg ccggtatcga ccgggtcgtg
       61 gtcgggcgtg gcggctgagc ctgttcggcc gaccgggctg actgccgggc ccgctctttc
      121 gccgcgggcg atcccggcct gacatgatcg ggttgtgaag gtcgtagcga caagcgtcaa
      181 cgccggcgac gccgcccgcc aggcactcga gtcctgcatc agcaagggcg gagtcgccct
      241 gtttccggcc gacggcctct acgggttggc ctgcgacccc ctgaacgaga aggcgattcg
      301 ccgcatccac gagatcaagg gccgcgatga cggcaagccg tcggcggtca tgtatctgtc
      361 accgctggca atgcgcgaac tgatccgcga cctcggtcag cgggtctccg aggcggccgc
      421 gaggctgctg cccgggccgg tcaccctggt cgtcccgaat ccccggcacc gctatccgct
      481 ggcctgccgc gacgacaatt cccggctcgg catcagggtg attgacgggc ccctgtccgg
      541 ggtgcgcttt ccgctcttcc agaccagcgc caacctttcc ggcgagccgg ctccctcggt
      601 cttcgagggc gttgtgcccg aggttcgcga aatggttgac ctggcgatcg acggcggccg
      661 gctgaccggc ctgccctcga ccgtggtcga cctgaccgac tatgacgagc acggcgaatg
      721 ggagatcctc cgcgaaggcg ggatgagccg gggcgacctg atcgccgccc tcggtcgtta
      781 gggattcatc gaatgcccgg tttgtgccgg tatgcggcgc aaagtggggt ctcgcggtgg
      841 tcgcagcggc agggtcagca gccgcggcga tcgtaaccgc gccaggggag gcggacgatt
      901 cgctgggcgt agttgttctt cttgctcaat tcgaagagca cgttgcccgg atcgaggtga
      961 agcacgtagg cgaagcagcc gcgcaggccg gtgacgttga tccactgccg gtcgtaggtc
     1021 gaggggtaga tgtcggacca gccgaccgaa gtgccaaggg tgacgtggcg gatccgcggg
     1081 ttctggttgc aggcgggata gaccgctgaa tcgggagagc gggtcgaggc cgggttggtc
     1141 tgaaccaggt cccggaagca gtagaactgc ttcggcccgg tacggaccag ttcgatcttc
     1201 ttgcctgccg cgttcacccg ccacagttcg aagcgcagcg ggttcctgat cttccagtag
     1261 gcgcctcccc actggtagcc cacgttgaag tagcgcaggc gcgcctgggt cggaaagaag
     1321 gtgtagccgc cgccgcggcg cctgatcgcc tgcgtcacgt tcatcgtgcg ggggcggtcg
     1381 cggtgaccac gcacctccat cggtcccatt ccgcggctgc ggatgtcatt ggtcgaacga
     1441 agcagggcct gtcccggtgc gaggtcccgg tcgaggtaca tctccgaagg cttgccgatg
     1501 cgaaggtccg ggcaaagcag cttttttgcg gttttcggag tccggcaggg gtcagttacg
     1561 ccgttcggct tgtcggggct gaaatcgccc cgggcagggg atcccgtacc gacgatcgct
     1621 gcgaacgaaa aggccagtaa cacccctgag aaagcggcca tcgacttcct gcgcatcttt
     1681 cgcatggtag tggagcgacc agcctcttcc cgcgtcgcta ggctcgacgc atgaaaattg
     1741 cgatgggatc ggatcacgcc ggattcgaac tgaaggaagc cgtcaaggcc cagctcgagg
     1801 acgccgggca cgaagtcgag gacgtgggga ccaattcctc cgaatcgacc gattacccgc
     1861 ccttcgcggc gaaggcggcc gaactggtgg ccagaggcga ggcggagaag ggagtgatcg
     1921 tctgcggctc cggagtcggc gtctcgatcg tcgccaacaa ggtcgacggg atccgcgcgg
     1981 tcaatgccca cgatgccgcg gaggccgaga tgagccgccg ccacaatgac gccaacgtgg
     2041 tcacgctctc cggggcgcgg ctcggtcccg aagatgcgaa gccgatcgtc gaaaccttcc
     2101 tcgccaccga tttcgacggt ggccggcacg ccagaagggt cggggagatc gccgaagtgg
     2161 agcgcggcga gctctaggct cgccggggct gccgccgcgc cccgctggaa cgcaacccgc
     2221 cgcaaattga cgccagccgc agcgcgtttc gcgaaccggg aaagccggaa aacgcggcat
     2281 agcgccgatt tcccggccga tccggaaccg gcctcgttgg taccctcgtt gctccccgtc
     2341 cacgaacgat ttcccagaaa gatggttgcc ttttcatgag caccgtttcc gaagagctgc
     2401 ttggcgccag cctgtccgag gtcgatcccg ctgttgcgga ggcgatcggc ggcgagctga
     2461 accggcagcg caatacgctt gagatgatcg cttcggagaa cttcgttccg cgggcggtgc
     2521 tcgaggcggc cggttcggtg ctgaccaaca agtacgccga gggctacccg ggccgccgct
     2581 actacggcgg ctgcgagcag gtcgacatcg tcgagcagct ggcaatcgac agggcgatgg
     2641 aactcttcgg cgccggccac gcgaacgtcc agccgcattc gggcgcccag gccaacaacg
     2701 ctgcctacat ggccctgctg gagccgggtg aaaccatcat gggcctcgcc ctcgaccacg
     2761 gtggccatct cagccacggg atgaagctga acgtctccgg caagctctac aacgtggttc
     2821 cgtaccacgt gtcccgtgag gacctgaggg ttgacatgga cgaggtcgcg aagctggccg
     2881 aggagaccaa gccgagcctg atcgttgccg gctggtcggc ctatccgcgt cagctcgact
     2941 ttgccgcctt ccgtgaaatc gccgattcgg tcggggcgaa gctgatgg
//
LOCUS       DAOQKN010000247         2986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_22281_length_2986_cov_5.353463, whole genome shotgun sequence.
ACCESSION   DAOQKN010000247 DAOQKN010000000
VERSION     DAOQKN010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22281_length_2986_cov_5.353463"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..917
                     /locus_tag="PLJ64_11950"
     CDS             141..917
                     /locus_tag="PLJ64_11950"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HNC94239.1"
                     /translation="MKASDAAPGAPVIIGPGRVGRSLAAAIGDGRVELRGRAEGLGDL
                     AGRIVLLCVPDGAIARVSEQIGAAGESPALIGHTSGATGLDALSGAGTEGSFSAHPLQ
                     TVPDGKTDLAGCPAAIAGSGPAALEVAARIAKSAGMKPFEVSEADRATYHAAASIASN
                     FLVTLEQTAAELMGGIAVENPREVLEPLVRRSLDNWLARGPEALTGPIARGDEATVEA
                     HRRALSEQRPELIPFYDALADRTREIAGNEAVAEGGRPWR"
     gene            908..1780
                     /gene="panC"
                     /locus_tag="PLJ64_11955"
     CDS             908..1780
                     /gene="panC"
                     /locus_tag="PLJ64_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNC94240.1"
                     /translation="MEVIRTPAAMHSLRDELRGQTVTVGLVPTMGYLHEGHNSLLKAA
                     RAENDAVVMSLFVNPTQFKAGEDLSTYPRDEERDLAIAESSGVDFIYAPLPDDVYPSG
                     FATKVEVEGLTEVLCGSETSRGPGHFAGVTTVVTKLLNAVDPDVAYFGQKDAQQATVI
                     RRMAEDLEFRTRIVILPTVRERDGLAMSSRNAYLSPEARERATAIWASLLETESVFSE
                     SGLDAALEAGRRHLQSAGITPEYFEARYPGTLDPAEGTGTDPILVAVAAKVDGTRLID
                     NILIEPSSTSRRNP"
     gene            1777..2718
                     /gene="panB"
                     /locus_tag="PLJ64_11960"
     CDS             1777..2718
                     /gene="panB"
                     /locus_tag="PLJ64_11960"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNC94241.1"
                     /translation="MSSGIRKEGEERRPVTLTRLAEMHEAGEPIAMITAYDFPSAQVI
                     EEAGADIVLVGDSAANCVLGYDSTVPVGMDEMTMLAKAVRRGLTTPFLVGDLPFGSYE
                     GSDELAIASAQRLVKEAGCDAVKLEGGGTSVERAKAIIDAGIPVMGHVGLTPQTATAL
                     GGMKAQGRTAERAIKVAEDAIALDQAGCFAVVIEAVPEAVSEMIVAKMTCPTIGIGAG
                     NATSGQVLVLHDLTGINHGHVAKFVKRYSDVRSEMLKGVEAYIAEVRDGSFPAPEHVY
                     SVDPSELEGLQQRLNREDLEHHSYFESTGSWDWEPLS"
BASE COUNT          523 a         1040 c          973 g          450 t
ORIGIN      
        1 tggctctgcc cgacatggcg gcagcctata attgccggtg gctgccaccc gggttccggg
       61 ggtggaccgt aaacccctca tcgcaggaaa aactcaactc gatccgtctg gtatcgccga
      121 aagggaactg gaacggcaac atgaaagctt cagacgcagc gcctggtgcg cccgtgatca
      181 tcggccccgg tcgggtcggc cggtcgcttg cggccgcaat cggtgacggc cgggtcgagc
      241 tccggggcag ggccgagggc ctgggtgacc tggccggccg catcgttctg ctctgcgttc
      301 ccgacggggc aattgccagg gtttccgagc agatcggcgc cgctggcgaa agcccggccc
      361 tgatcggcca caccagcggc gcgaccggtc tcgatgccct ttcgggggcc ggcaccgagg
      421 ggagcttctc ggcccatccc ctgcagacag tccccgacgg caagacggat ctcgccggct
      481 gcccggcggc gatcgccggt tccgggccgg ccgcacttga agtcgcggcc cggatcgcga
      541 agagtgcagg gatgaagccc ttcgaggtat cggaagccga ccgcgcgacc taccacgccg
      601 cggccagcat cgcttcgaac ttcctcgtga ccctcgaaca gaccgcagcc gagctcatgg
      661 ggggcatcgc ggtcgagaac ccgcgcgagg ttctcgagcc gctggtccgt cgcagcctcg
      721 acaactggct ggcccggggg ccggaggcgc tgaccggccc gatcgcccgc ggggatgaag
      781 ccacggttga agcccatcgc cgggccctga gcgagcagcg tccggagctg atccccttct
      841 atgacgccct ggccgatcgc acccgagaga tcgccggcaa cgaggccgtc gcggaaggcg
      901 gccgaccgtg gaggtaatcc gcaccccggc cgcgatgcat tccctgcgcg acgagctgcg
      961 cggacagacc gtgacggtgg gcctggtccc gaccatgggc tacctccatg agggccacaa
     1021 ctcgctgctc aaggcggccc gggccgagaa tgacgcagtc gtgatgagcc tcttcgtcaa
     1081 cccgacccag ttcaaggccg gcgaggacct cagcacttac ccccgcgacg aggagcggga
     1141 cctggcgatc gccgaatcct ccggcgtgga tttcatctac gcaccgctgc cggatgacgt
     1201 ctacccctcc ggtttcgcga ccaaggtcga agtcgagggg ttgaccgaag tgctctgcgg
     1261 cagtgaaacc agccgtggcc ccggccactt cgccggagtg accacggtgg tcacgaagct
     1321 gctcaatgcg gtcgatcccg acgtcgccta cttcggccag aaggatgccc agcaggccac
     1381 ggtgatccgc cgcatggccg aggacctcga gttccgcacc aggatcgtga tcctccccac
     1441 cgtccgcgag cgtgacggcc tggcgatgag ttcacgcaat gcctacctct caccggaggc
     1501 tcgtgaacgc gccaccgcga tctgggcctc gctgctcgaa accgaatcgg tcttttccga
     1561 gtccggcctt gacgccgcac tcgaagccgg ccgccggcat ctccagtcgg ccggcatcac
     1621 ccccgaatac ttcgaggccc gctatccggg caccctcgat ccggcggaag gaacgggcac
     1681 cgacccgatc ctcgtcgccg tcgccgccaa ggtggacggc acccgcctga tcgacaacat
     1741 cctgatcgaa ccatcaagca cctccaggag gaacccatga gctccggaat ccgcaaagaa
     1801 ggcgaagagc gccgccccgt aaccctgacc cggctggccg aaatgcatga ggccggcgag
     1861 ccgatcgcga tgatcaccgc ctatgacttc ccctcggccc aggtgatcga ggaggcgggc
     1921 gccgacatcg ttctggtcgg tgattcggcc gcgaactgcg tgctcggcta cgactcgacc
     1981 gtgccggtcg gcatggacga aatgaccatg ctggccaagg ccgtgcgccg gggccttacg
     2041 acccctttcc tggtcggcga cctgcccttc ggctcctatg agggctcgga cgaacttgcc
     2101 atcgccagcg cccagcgcct ggtcaaggaa gccggctgcg atgcggtcaa gctcgaaggc
     2161 ggcggcacat cggtcgagcg cgcgaaggcg atcatcgacg ccggaatccc ggtgatgggt
     2221 catgtcggac tcaccccgca gaccgcgacc gccctcggtg gcatgaaggc ccagggccgg
     2281 accgccgaac gggcgatcaa ggtggccgag gacgcgatcg cgctcgatca ggccggctgt
     2341 ttcgcggtgg tcatcgaagc ggtgccggag gcggtgtcgg agatgatcgt cgccaagatg
     2401 acctgcccga cgatcgggat cggggccggc aacgcaacct ccggccaggt cctcgtcctg
     2461 catgacctga ccgggatcaa tcacggccat gtagccaagt tcgtcaagcg ctattcggat
     2521 gtccgttcgg agatgctgaa gggcgtggag gcctacatcg ccgaggtccg ggacggctcc
     2581 ttccccgccc ccgaacatgt ctacagcgtc gacccctcag aactggaagg cctccagcag
     2641 cgcctcaacc gcgaagacct cgaacaccac tcctacttcg agtcaaccgg ctcctgggac
     2701 tgggaaccgc tctcctgagc tccatgcgtt ggaggtaacg ccgtggggtc gggggcatgc
     2761 ccgcccgacc ccgcggctac accaacccgg attccaggca cgaaggtgcc gcaggtttgc
     2821 gacctctccg gtagcaaacc tgcggcacct cagagtccaa agacccgcga agggaggcat
     2881 ctcccgattt ggagtagccg tggggtcggg tgagggtcgt ccgggacact ccaggaccag
     2941 cgcggctgaa tgccgcgcgt gcgtccgtcc cggcgatcct cacccg
//
LOCUS       DAOQKN010000248         2935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_22958_length_2935_cov_6.360069, whole genome shotgun sequence.
ACCESSION   DAOQKN010000248 DAOQKN010000000
VERSION     DAOQKN010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22958_length_2935_cov_6.360069"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1344)
                     /locus_tag="PLJ64_11965"
     CDS             complement(<1..1344)
                     /locus_tag="PLJ64_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNC94242.1"
                     /translation="MAVVTATSIGIHLGGDPLFSEVSFKLMRGDRMTLSGRNGAGKST
                     LLRILAGEAENDSGSLSFQKGARIALHDQRPPRDQGLSLEEYVYSGRSDVIATERELE
                     RLEAAMSAGEHDDETMRAYAAAQQQLELAGGYRWRDEVLTVLRGIGFGEEEFPRPLSS
                     FSGGELTRASLARALGARADLLLLDEPTNHLDIPTLEWLEEYLKDLDASVVLVAHDRW
                     FLESVGTSVLELEAGKARFFAGPWHAWRKEQAARQVALGKQIERQQAEIARMERFVER
                     FRYKATKARQAQSRVKQIEKVKKNAASIDPQDRRVLAFDFAKPERASQVVLKMVNGRI
                     EVPGRVLLEEANMLLERDEHVVLVGPNGAGKTTLMATLAGDRKPAAGHVRLGHNAKVG
                     YLSQHAETATGDGTVLDAAARETGLTGQKVRDLLGAFLFSGEDANKQLSDISGGEQ"
     gene            1483..>2935
                     /locus_tag="PLJ64_11970"
     CDS             1483..>2935
                     /locus_tag="PLJ64_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HNC94243.1"
                     /translation="MSNGGFDPHPHSGPQYGQTWEARIQSPEAVHGVSPYPPIADYGF
                     LSDGEVSALVAPSGNIEWLCLPRFDSPSIFGSILDRSAGNFRLGPADINVPSHRRYLP
                     GTNVLETSWGTGDGWIIVRDVLLIGPWHHERDRSGTHRRAPTDYDADHVLLRMVRCVH
                     GEVQLTLDCKPVFDYGRVPAQWEYTGDAYHEGLATAPGIDLQLRLNTDMNLGFEGPRT
                     TARTMLKEGDVRFVALSWSEHQAPQHYDEAYDRLVWTAHHWQNWLARGTFPDHPWRGY
                     LERSALTLKGLTYAPTGALVAAATTSLPETPGGERNWDYRFSWIRDSTFTLWGLYTLG
                     FDWEANDFFYFIADVAAGEDDLQIMYGVAGEKELREEILEHLHGYEGASPVRIGNGAY
                     DQRQHDVWGAVLDSFYLHTKSRDGMPEEIWPILKRQVNAALEHWRETDHGIWEVRGKP
                     QHFTSSKVMCWVAVDRGARLARLREEHDLADQWR"
BASE COUNT          491 a         1004 c          924 g          516 t
ORIGIN      
        1 ttgctcaccg cccgagatgt cgctcagctg cttgttcgcg tcttcgcccg agaaaagaaa
       61 ggcgccgagc aggtcccgga ccttctggcc ggtgagcccg gtttcgcggg cggccgcgtc
      121 gagcacggtg ccgtcgcccg tagcggtctc cgcgtgctgc gagaggtaac ccaccttggc
      181 gttgtgacca agccggacat gcccggcggc gggctttcga tcgccggcaa gggtcgccat
      241 cagcgtcgtc ttgccggccc cgttcggacc caccagcacc acatgctcgt cacgctcaag
      301 cagcatgttc gcttcctcga gcagcacccg gcccggcacc tcgatccggc cgttgaccat
      361 tttcagcaca acctggctcg ctctttccgg cttggcaaaa tcaaaggcga gcacccttcg
      421 gtcctgcggg tcaatgctcg ctgcgttctt cttcaccttc tcgatctgct tgacccggga
      481 ctgggcctgg cgggccttcg tcgccttgta gcggaaccgc tcgacgaagc gctccatgcg
      541 ggcgatctcg gcctgctgcc tttcgatctg cttgccgagc gcgacctgcc gcgcggcctg
      601 ttccttgcgc caggcatgcc aggggccggc gaagaaccgg gccttgccgg cctccagttc
      661 cagcaccgag gtgcccaccg attcgagaaa ccagcggtca tgcgcgacca gaacgacgct
      721 ggcatccaga tccttcagat actcctcaag ccactcgaga gtgggaatgt caaggtggtt
      781 ggtcggctcg tccagcagca gcaggtcggc acgggcacca agggcccggg cgagggaggc
      841 gcgggtcagc tctccgccgg agaagctgga aaggggccgg ggaaattcct cttcgccgaa
      901 gccgattccc cgcagcacgg tcagcacttc gtcgcgccag cggtagccgc cggccagttc
      961 gagctgctgc tgggccgcgg cgtaggcgcg catcgtctcg tcgtcatgct cgccggcaga
     1021 catggcagct tcgaggcgtt ccagttcccg ctcggtcgcg atcacgtcgg agcggcccga
     1081 gtagacgtat tcctcaaggg aaagcccctg gtcgcgcggt ggacgctggt cgtgaagtgc
     1141 aatccgggcg cccttctgaa aactgagaga tcccgagtcg ttttcagctt cgccggccag
     1201 aatccgcagc agggtcgact tcccggcccc gttgcggccc gaaagcgtca tgcggtcacc
     1261 gcgcatgagc ttgaaggaga cctcggagaa gagcggatcg cccccgagat ggatcccgat
     1321 tgatgttgcg gtgactactg ccatggagcg aaaccatagg tagtcggcgc cggcaacgcc
     1381 cggcctcgtt tgaagacccg cccgccgatc gggcgggttt ccgcttgcca cccggtgaag
     1441 accgggtggc ttgctacgga aaatgtgaca gcattttccg caatgagcaa tggcggcttt
     1501 gacccccacc cccactcggg accccagtac gggcagacct gggaagcccg gattcagtct
     1561 ccggaggccg ttcacggggt ctcgccatac ccgccgatcg ccgactacgg cttcctttcg
     1621 gacggcgaag tttcggccct ggtcgcgccc agcggcaaca tcgagtggct ttgcctcccc
     1681 cgcttcgact cgcccagcat cttcggttcg attctcgatc gcagcgccgg aaacttccgg
     1741 cttggcccgg ctgacatcaa cgtgccgagc caccgccgct acctgcccgg caccaatgtg
     1801 ctggaaacca gctggggcac gggtgacggc tggatcatcg ttcgcgacgt cctgctgatc
     1861 gggccctggc accacgagcg ggaccgctcc ggaacccacc gccgcgcccc gaccgactat
     1921 gacgctgacc acgttctgct gagaatggtg cgctgcgtcc acggcgaagt ccagctgacc
     1981 ctcgactgca agccggtctt cgactatggc cgggtccccg cccagtggga gtacacgggt
     2041 gacgcctacc acgagggtct ggccaccgcg ccggggatcg acctccagct gcgcctcaac
     2101 accgacatga acctcggctt cgagggtccg cgcaccactg cccggaccat gctcaaggag
     2161 ggtgacgtcc ggttcgtcgc cctgtcctgg agcgaacacc aggccccgca gcattacgac
     2221 gaggcctacg accggctggt ctggaccgcc catcactggc agaactggct ggcccggggc
     2281 accttccccg accatccctg gcgcggctac ctggagcgtt cggccctgac cctgaagggg
     2341 ctgacctatg ccccgaccgg agccctggtc gcggccgcca ccacctcgtt gcccgaaacc
     2401 cccggcggtg aacgcaactg ggattaccgg ttctcctgga tccgcgattc aaccttcacc
     2461 ctctggggcc tttacacgct cggcttcgac tgggaggcaa acgacttctt ctacttcatc
     2521 gccgatgtcg cggcgggcga ggacgacctc cagatcatgt acggggtcgc cggtgaaaag
     2581 gagctcaggg aggagatcct cgaacacctt cacggttacg agggcgcctc accggtccgg
     2641 atcggaaacg gtgcctacga ccagcgccag catgatgtct ggggcgcggt tctggattcc
     2701 ttctatctgc acacgaagtc ccgcgacggg atgccggagg agatctggcc gatcctcaag
     2761 cgtcaggtca acgccgcgct cgagcattgg cgtgaaaccg atcacggaat ctgggaggtt
     2821 cgcggcaagc cgcagcactt cacttcgtca aaggtgatgt gctgggtagc ggttgaccgc
     2881 ggtgcccggc tggcccggtt gcgggaggag catgacctcg ccgaccagtg gcggg
//
LOCUS       DAOQKN010000249         2883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_23689_length_2883_cov_4.753182, whole genome shotgun sequence.
ACCESSION   DAOQKN010000249 DAOQKN010000000
VERSION     DAOQKN010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23689_length_2883_cov_4.753182"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1320)
                     /locus_tag="PLJ64_11975"
     CDS             complement(<1..1320)
                     /locus_tag="PLJ64_11975"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNC94244.1"
                     /translation="MFSKILIANRGEIAIRVARACKEMGITSVAVYSEADRDAPHVKA
                     CDEAFLIGPAVPAESYLSIEKIIQAAKDSGAEAVHPGYGFLAENADFARALEDAGIVF
                     IGPPASAIDAMGSKTMAREVMEKAGVPIVPGATEPAKDLDEARAQAEAAGYPVACKAV
                     GGGGGKGFRVAMTPDDLQEAFEGSGREGMKFFNDDRVYIERYLEDPRHVEVQVLADKH
                     GNVIHLAERDCSIQRRHQKLIEEAPGPHVDEEMRERIGKIATDAARAVGYFSAGTIEG
                     MQHGDEYFFLEMNTRVQVEHCVTEEITGVDIVREQIKIAYGEELSLKQEDVKIDGWAI
                     ECRINAEKAHMNFAPAPGPIEVYDEPSGPGVRVDSGVKAGSEVTPMYDPMVAKLIVWD
                     KDREAATRRMLRALDEYEIAPLSTLIPFHKTILRTEQWARGETCKDLT"
     gene            1758..2561
                     /locus_tag="PLJ64_11980"
     CDS             1758..2561
                     /locus_tag="PLJ64_11980"
                     /inference="COORDINATES: protein motif:HMM:NF037405.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HNC94245.1"
                     /translation="MTKLFHGIYAYGRDIETRESAWRAALLVAGPGSALTSRSALELL
                     GAARLLTPIPPVIHVATSRDRAGRHRGLSPALRNTWVYVVRRRFDPGDLRERGGLAVV
                     RAALALLDFAVSAPDTDVKFAFLELCRLRLFGEKDVQFCFRRSVGKRGALKVRPLLGL
                     WVPELNRIKSVLEGLFLLSWVPRRWKLPEVNVKIFGREVDFLWRKEGLVLELDGDAFH
                     TDPIARQRDLEKTRFLESKGLTVIRITWKEFMEDPDGVVERIARELGLV"
BASE COUNT          473 a          877 c          966 g          567 t
ORIGIN      
        1 ggtcaggtcc ttgcaggtct cgccacgggc ccactgttcg gtccggagga tcgtcttgtg
       61 gaacgggatc agggtcgaga gcggcgcgat ctcgtactcg tcgagcgccc gcagcatccg
      121 cctggtcgcg gcctcgcggt ccttgtccca gacgatcagc ttggcgacca tcgggtcgta
      181 catcggggtg acctcggaac cggccttgac gccggaatcg acccggacgc ccggaccgga
      241 aggctcgtcg tagacctcga tcgggcccgg agcgggggcg aagttcatgt gggccttctc
      301 cgcgttgatg cggcattcga tcgcccagcc gtcgatcttc acgtcttcct gcttgaggct
      361 cagttcctcg ccgtaggcga tcttgatctg ctcccggacg atgtcgaccc cggtgatctc
      421 ctcggtcacg cagtgctcga cctggacccg ggtattcatc tcgaggaaga agtactcgtc
      481 gccgtgctgc atgccttcga tcgtgccggc cgagaagtaa ccgacggcgc gggccgcgtc
      541 ggtcgcgatc ttgccgatcc gctcgcgcat ctcttcgtcc acatgcgggc cgggggcctc
      601 ttcgatcagc ttctggtgcc ggcgctggat cgagcagtcg cgctcggcga ggtggatcac
      661 gttgccgtgc ttgtcggcca gcacctggac ttcgacgtgg cgcgggtcct ccaggtagcg
      721 ctcgatgtac acgcggtcgt cgttgaagaa cttcattcct tcgcggccgg agccctcgaa
      781 ggcctcctgc agatcgtccg gggtcatggc gacgcggaag cccttgccgc cgccgccacc
      841 gaccgccttg caggcgaccg ggtagccggc cgcttcggcc tgggcgcggg cctcatcgag
      901 gtccttggcg ggctccgtcg cgccgggcac gatcggcacg ccggccttct ccatcacttc
      961 gcgggccatc gtcttcgaac ccatcgcgtc gatcgccgag gcgggcgggc cgatgaaaac
     1021 tatcccggca tcttccagtg cccgggcgaa gtccgcgttc tcggccagga agccgtagcc
     1081 ggggtgaacc gcctcggccc cggagtcctt cgcggcctgg atgatcttct cgatcgagag
     1141 gtaactctcg gccggaaccg ccggtccgat caggaaagct tcgtcacagg ctttgacgtg
     1201 cggagcatcc cgatccgcct ccgaataaac ggcgacggag gtaatcccca tctccttgca
     1261 agcccgggcg acacggatag cgatttcgcc acgattggcg atcaggatct tcgaaaacat
     1321 agcccgggca gtctatttat ccgccccggt cccggctttg cccccgctcc ctcccagcca
     1381 gcccgcgcag agccatccga tccaacccag ccccagaagc cagccccaga atcgagaagg
     1441 caaaagtggc catatagcgc tccatttagc ctcctcgccg gtcgcggctc gaaccggcga
     1501 gttcaaggca ggcttcgcgg gcgtttcagg ctcccgggac gttgcacaat tgtcaccctt
     1561 atagagccac gtgtgagcat ttctcgggca ggcttgggcc gatgaattcg gtctccgggg
     1621 aaagtccatc cagcgccatc gagcttgacc ggctttggca gttcatgtcc cggcgccgtc
     1681 atgttgccgg gagggatgaa ctcattcgtc atggattcag tccgtccagg atcaagaatt
     1741 ggctcagagg caggcggttg accaaactct tccacggcat ctatgcctac gggcgggaca
     1801 tagagaccag agagtcggcc tggcgggcgg ctctcctggt tgcgggtccc ggttctgccc
     1861 tgacctcacg gagtgccctg gagctcctgg gggcggcgcg cctcttgact ccgatcccgc
     1921 cagtgatcca cgtggctaca tccagagacc gcgctggcag gcaccgaggg ctgtcgcccg
     1981 ctctcaggaa cacctgggtc tatgtggtcc gccgacggtt cgacccgggt gacctgcgcg
     2041 agagaggcgg ccttgcggtc gtgcgagcag ctttggccct gctcgacttt gccgtaagtg
     2101 ctcctgacac agacgtgaaa ttcgctttcc tggagttgtg ccgactccgg ttgtttggcg
     2161 aaaaggacgt gcagttctgc ttccgccggt cagtgggaaa acgaggcgcg ctaaaggtga
     2221 ggccgttgct cgggttgtgg gtgccggaac tgaaccggat caagtccgta ctcgaggggc
     2281 tgtttctcct gtcgtgggtg ccgcgacggt ggaagctgcc ggaggtgaac gtgaagatct
     2341 tcgggcgaga ggtcgacttc ctttggcgaa aagaaggtct cgtgctggag ctcgacggag
     2401 atgccttcca caccgatccc atcgccaggc aacgggacct ggagaagacc cgtttcctgg
     2461 aaagcaaggg acttacggtc atccggatca cctggaagga gttcatggaa gatccggacg
     2521 gtgtggtcga gcggattgcc cgcgaactcg gactggtctg agagtcccgc cagcgaagtg
     2581 gtcgagcggc tgggtgatga gccgagaagg ctgaatggag cgctatatgg ctacttttgc
     2641 cttctcgatt tttggggagg gagttcgagg gggattgagg ggaggagggg ttgaaacggt
     2701 gggggcttgt ggggcggagc ggaattcagg ggtggagctt aggattcggg gggatgatcg
     2761 aacttcagtc tcattccgtt gtctctgatg gccagcttga accggcgggg gtagtcgagg
     2821 aggcggcgaa ggccggggtc gaggtgctcg ccctgaccga tcacgacggg gtggcgggtg
     2881 tgc
//
LOCUS       DAOQKN010000250         2872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_23837_length_2872_cov_6.242812, whole genome shotgun sequence.
ACCESSION   DAOQKN010000250 DAOQKN010000000
VERSION     DAOQKN010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23837_length_2872_cov_6.242812"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="PLJ64_11985"
     CDS             complement(<1..361)
                     /locus_tag="PLJ64_11985"
                     /inference="COORDINATES: protein motif:HMM:NF019499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HNC94246.1"
                     /translation="MLEDQRFIISRDIPIEVGGLFILVALVGIGFAIWGYRTGGPRGE
                     QILRATAGVLSGLGLFVAGYIAYKTQIVNEPFQCAGGGGGCALVEKSKYANFLGVHMS
                     IWGLIGYATILGATIWKG"
     gene            complement(374..967)
                     /locus_tag="PLJ64_11990"
     CDS             complement(374..967)
                     /locus_tag="PLJ64_11990"
                     /inference="COORDINATES: protein motif:HMM:NF019499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HNC94247.1"
                     /translation="MNDQLYLLTHGIPGVIAGIMLALTLIGVSGSIWGIKRGLDGGEK
                     ILRVTAAILCLVGIGIAGYVVVTVEIMNQIPECVGGGGGCAAVEHSSYSRIGGIHVSI
                     FGLIGYTMLLVTSFMKGDLARIGAFFLALFGFGFSLYLTYLELWEILAICQWCVSSAI
                     TMTMLFVVNTSRLIVFFGTGESNGLAEGVEDPGPSSV"
     gene            complement(980..>2872)
                     /locus_tag="PLJ64_11995"
     CDS             complement(980..>2872)
                     /locus_tag="PLJ64_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein meaA"
                     /protein_id="HNC94248.1"
                     /translation="KGQTGLSIAFDLPTQTGYDPDHALSRGEVGKVGVSVAHLGDMEA
                     LLDQIPLDNMNTSMTINAVAAWMLGLYVAAAEETGVDKSQLKGTTQNDIIKEYLSRGT
                     YAFPPEPSMRLIADMVAFTVNEIPSWNPINICSYHLQEAGATPIQEIAYAMSTAIAVL
                     DEVKARGQVSEEDFPKVFGRISYFVNAGVRFIEEIAKLKAMGRLWTEIGRERYGVTDE
                     KMLRFRYGVQVNSLGLTEAQPENNIQRIVVEALAVTLARDARARALQLPAWNEAMGLP
                     RPWDQQWSLRIQQVLAFETDMLEYPDIFEGSVVMEGLVEEFMAGARDEMAVVAEKGGA
                     VEAVPYMKARLVESHRERFARIENGEQTVVGLNKYTETEDSPLTASADGGIMKPDPQV
                     EKERIEALEAWRSERDQAAVDAALAEVARVAGADDENIMPATIAAAKAGATTGEWAAT
                     LREVFGAYRGPTGVGGAAPAGDRDSLTATREKVNEVSGQIGHRIRLLVGKPGLDGHSN
                     GSEQIALRARDVGMEVVYQGIRLTPEQIVESAVQEDVDLIGLSILSGSHLELIPEVVR
                     LLQEEGVEVPVVAGGIIPPEDAARLKEAGVAGVFTPKDFDMNEIMGEFVDLVAANHEK
                     SAAATA"
BASE COUNT          513 a          958 c          908 g          493 t
ORIGIN      
        1 cacccttcca gatcgtcgct ccgaggatcg tcgcgtagcc gatcagcccc cagatcgaca
       61 tgtgtacccc gaggaagttc gcgtacttgc tcttctcgac cagggcgcag ccgccaccgc
      121 cgccggcgca ctggaagggc tcgttcacga tctgggtctt gtaggcgatg tagccagcca
      181 cgaacagtcc gagcccggaa aggacaccgg ccgtcgcacg gaggatctgc tcgccgcgcg
      241 gaccaccggt ccggtaaccc catatcgcga aaccgatgcc caccagagcg acgagaatga
      301 agaggccgcc gacttcgatc ggaatgtccc ggctgatgat gaaccgctgg tcttcgagca
      361 tggggaagcg agcctacaca gacgacgggc cagggtcttc cacgccttca gccagaccgt
      421 tgctctcgcc ggtgccaaag aacacgatca acctgctcgt attgaccacg aacaacatgg
      481 tcatggtgat cgccgaggaa acgcaccact ggcagatcgc gagaatctcc caaagctcga
      541 gataggtcag gtagaggctg aacccgaaac cgaagagagc cagaaagaaa gcgccgatcc
      601 gggccaggtc acccttcatg aaagaggtga ccagcagcat cgtgtagccg atcaggccga
      661 agatcgaaac gtggatcccc ccgattcgcg agtaggagct gtgctcgacc gcggcgcaac
      721 cgccgccacc gccaacgcat tccggaatct gattcatgat ttccaccgtc acgaccacgt
      781 aaccggcgat tccgatcccg accaggcaga ggatcgcggc ggtgacccgg aggatcttct
      841 cgccgccgtc gaggccgcgt ttgatccccc agatcgaacc gctgaccccg atcagggtca
      901 acgccagcat gatcccggcg attacaccgg ggattccgtg agtcagcaga tagagctgat
      961 cattcacgga acctcctgcc taggccgttg cggccgccga cttctcgtga ttggcggcga
     1021 ccaggtcgac gaactcgccc atgatctcgt tcatgtcgaa gtccttcgga gtgaagaccc
     1081 cagccactcc ggcttccttc agccgggccg cgtcctcggg cgggatgatg ccgccggcga
     1141 ccaccggcac ctcgacgccc tcctcctgaa gcagtcgcac gacctcggga atcagctcga
     1201 ggtgcgatcc tgaaaggatc gagagtccga tcaggtcaac gtcctcctgg accgccgatt
     1261 cgacgatctg ctccggagtg aggcgaatgc cctggtagac gacttccatg cccacgtcgc
     1321 gggcgcgaag cgcaatctgc tcggagccgt tcgaatggcc gtcaaggccc ggtttgccga
     1381 ccagcagccg aatccggtgg ccgatctgcc cggaaacctc gttgaccttc tcccgggtcg
     1441 cggtcagcga atcgcgatca ccggccgggg cggcgcctcc gacaccggtc ggaccccggt
     1501 aggcgccgaa gacctcgcgc agggtggcgg cccactcgcc ggtggtcgca ccggccttgg
     1561 cggcggcgat ggtggccggc atgatgttct cgtcgtcggc gccggcgacc cgggcgactt
     1621 cggccagggc agcgtcgacc gcggcctggt cacgctcgga gcgccacgct tcaagcgcct
     1681 cgatccgctc cttctcgacc tgcgggtcgg gcttcatgat gccgccgtcg gcactcgccg
     1741 tgaggggcga atcctcggtc tcggtgtact tgttcaggcc gaccacggtc tgctcgccgt
     1801 tctcgatccg ggcgaagcgc tcgcggtggg attcgaccag gcgggccttc atgtacggga
     1861 ccgcctcgac cgcgccgccc ttctcggcga ccacggccat ctcgtccctg gcgccggcca
     1921 tgaactcctc gaccaggcct tccatcacca ccgagccctc gaagatgtcc gggtattcga
     1981 gcatgtcggt ctcgaaggcc agcacctgct ggatgcggag gctccactgc tgatcccacg
     2041 ggcgggggag acccatcgcc tcgttccagg ccggcagctg cagcgcgcgg gcccgggcgt
     2101 ccctggccag ggtcacggcc agggcctcga ccacgatccg ctgaatgttg ttctccggct
     2161 gggcttcggt caggccaagc gagttgacct ggacgccgta acggaagcgc agcatcttct
     2221 cgtcggtgac gccgtaacgc tcgcggccga tttcggtcca gagccgaccc atcgccttga
     2281 gtttcgcgat ctcctcgatg aagcggacac cggcgttgac gaagtaggag atccggccga
     2341 agaccttggg gaagtcctcc tcggaaacct ggccgcgggc ctttacctcg tcgagcaccg
     2401 caatcgcggt ggacatcgcg taggcgatct cctggatcgg ggtggccccg gcttcctgca
     2461 ggtggtaact gcagatgttg atcgggttcc agctcggaat ctcgttcacc gtgaaggcga
     2521 ccatgtcggc gatcagccgc atcgagggct ccggcgggaa ggcgtaggta ccgcgggaga
     2581 ggtactcctt gatgatgtcg ttctgggtcg tgcccttgag ctgggacttg tcgacgccgg
     2641 tttcctcggc ggcggcgaca tagagcccga gcatccaggc ggcgaccgca ttgatcgtca
     2701 tcgaggtgtt catgttgtcc agcgggatct gatccagcag ggcctccatg tccccgagat
     2761 gcgccaccga gacgccgacc ttgccaacct cgccgcgcga aagggcgtga tcggggtcat
     2821 agccggtctg tgtgggcagg tcgaaggcga tcgagaggcc ggtctggccc tt
//
LOCUS       DAOQKN010000251         2808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_24876_length_2808_cov_4.060298, whole genome shotgun sequence.
ACCESSION   DAOQKN010000251 DAOQKN010000000
VERSION     DAOQKN010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24876_length_2808_cov_4.060298"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="PLJ64_12000"
     CDS             <1..324
                     /locus_tag="PLJ64_12000"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNC94249.1"
                     /translation="IDDEIFGPVFNLIPVDGEAQAIEVANSSSFGLMASVFSADPQRA
                     LGVAEVLQSGGVVINGTDNYRPPVIPFGGVKLSGRGVEGIGYTLEEMTREKPIIFRRF
                     RARGE"
     gene            353..1174
                     /locus_tag="PLJ64_12005"
     CDS             353..1174
                     /locus_tag="PLJ64_12005"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HNC94250.1"
                     /translation="MDSNGGSRQEAGEKPAKYQVPAVVGAISVLHEVATSDEPGVTQA
                     ELVKSTGLSKSTMHNLLATLEAHDFVRRENNSRNYRLGPALIPLGTEASRQVRLVRST
                     IDRVAPLAAEYHLSFAVAQVVSDEECRIIERFYPTEDVHVGILIGSSYGPMDGALGKV
                     LLAYMDPAKARRIVKSRKLPAHTDATITSPENLIETVEEAKVRGWAISAGELNQNHAV
                     ATGVLGQNGELALLLLALGFPADLDDDRMGEIGQLLAGIAASIMADAGISARSEA"
     gene            1402..>2808
                     /locus_tag="PLJ64_12010"
     CDS             1402..>2808
                     /locus_tag="PLJ64_12010"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNC94251.1"
                     /translation="MDGPPVVFVSNCDLLSRPGSQQAGVDIELAGGTVRRIGPTGESP
                     APAGAELIDGTGLLAMPGLVNAHSHSPENCLRGVGEGLRLEPWLLRMFGSSGLYSPED
                     HYVNALAGAVEMLLLGVTTVVDHLWMTPPSLDAVEGAVRAYEEAGIRAAVAPLMNDCD
                     FTGELAAASDFDLGPAFMSEQVAFLSSEELLAQFEQAVARWHGSAGGRIRILAGPGGV
                     QWCSDELLGGLADAARSHGTGLHIHLLETSLQRAVCDQRFGRSGVEALEDLGVLGPNC
                     SLPHSVWIDDEDIERIARTGSIVVHNPAANLRLGSGRCPVGRLLEREATVAIGTDGSA
                     SSDNQNVWEMVKLGALIHNDGPRWVSGADSLTMATGSGYQVWSGGEAGLSLGGEIAVG
                     AVADLILLNRSTDGLAGVQVLEPGLALSESGRSVKHVIVDGRVVVRDGNLATADGAAV
                     REALREQVQRRSREVASPP"
BASE COUNT          440 a          953 c          957 g          458 t
ORIGIN      
        1 atcgacgacg agatcttcgg cccggtcttc aacctgatcc cggtcgacgg cgaggcgcag
       61 gcgatcgagg tcgccaactc ctcctccttc ggcttgatgg cctcggtctt ctcggccgat
      121 ccgcagcggg cgctcggcgt ggccgaggtg ctccagtcgg gcggggtggt gatcaacggc
      181 accgacaact accggccgcc ggtcattccc ttcggcgggg tgaagctctc cggtcgcggg
      241 gtcgagggca tcggctacac gctcgaggag atgaccaggg agaagccgat catcttccgc
      301 cgtttccgcg cccgcgggga gtagggtcac cggaggagct gggaagggtc acatggatag
      361 caacggaggg tcaagacagg aagccggcga gaagccggcc aagtatcagg ttccggccgt
      421 ggtcggggcg atcagcgtgc tgcatgaagt agccacgagc gacgagcccg gggtcacgca
      481 ggcggagctg gtcaagtcca ccggtttgtc caagtcgacc atgcacaacc tgctggcgac
      541 cctcgaggcc cacgacttcg tgcggcgcga gaacaattcc cgcaactacc ggctcggccc
      601 cgccttgatc ccgctcggga ccgaagccag ccgtcaggtc cggctggtcc gctcgaccat
      661 agaccgggtc gcgccgctgg ccgccgagta ccacctctcg ttcgcggtgg cccaggtggt
      721 cagcgacgag gagtgccgga tcatcgaacg cttctacccg accgaggacg tccacgtcgg
      781 gatcctgatc ggttcctcct acgggccgat ggacggcgcc ctgggcaagg tgctgctggc
      841 ctacatggac ccggcgaagg cacgacggat cgtcaagtcc agaaagctgc cggcgcatac
      901 cgacgcgacc atcaccagcc ccgagaacct gatcgagacg gtcgaagagg cgaaggtccg
      961 cggctgggcg atcagcgccg gcgagctgaa tcagaatcac gccgtggcga ccggtgtgct
     1021 cggtcagaac ggcgagctcg ccctgcttct gctcgcgctc ggtttcccgg ccgacctcga
     1081 cgatgaccgc atgggcgaga tcggccagct gctcgccggg atcgccgcgt cgatcatggc
     1141 cgacgcgggg atcagcgccc gctccgaagc ctgactcacc cctcgtaaga ccgcttccct
     1201 ccacccgtcg agaggccggc cgaacagccg ggaagtcgtc ctgcgcgaac ggatatcgag
     1261 atttctttgc aagtttcgat agccggtgct atgtttcatt cctagaactg agttcaagtt
     1321 attgaacttt gtttgcccca ggtcacggga agagaggagc cggtgaccgc tccccacagg
     1381 aggcttgtcg agttgcaccg cgtggatggc ccgcccgtcg ttttcgtcag caactgcgac
     1441 ctgctctcac gccccggctc gcagcaggcc ggcgtagaca tcgagttggc ggggggcacc
     1501 gtccgtcgga tcggtccgac cggtgagtca cccgctccgg caggcgccga gctgatcgac
     1561 ggcaccgggc tgctggccat gccgggcctg gtcaacgccc actcgcactc cccggagaac
     1621 tgcctgcgag gcgtcggcga gggcctgcgc ctcgagcctt ggctcctgcg gatgttcggt
     1681 tcttccggtc tctacagtcc cgaggaccac tacgtgaacg cgcttgccgg ggcggtcgag
     1741 atgctgttgc tcggggtcac cacggtggtc gaccacctct ggatgacgcc gccctcgctc
     1801 gacgcggtcg agggtgcggt tcgcgcctac gaggaagccg ggatccgggc cgcggtcgcg
     1861 ccgctcatga acgactgcga cttcacgggc gagcttgccg ccgcatccga cttcgatctc
     1921 gggccggcct ttatgtccga acaggtcgcc ttcctcagca gcgaggaatt gctcgcccag
     1981 ttcgagcagg cagtggctcg ctggcacggt tccgccggtg gccgcatccg catcctggcc
     2041 gggcccggcg gggtgcagtg gtgctccgac gagctgctcg gcggactcgc cgatgccgcc
     2101 cgctcccacg gcaccggcct gcacatccat ctgctcgaga cctcgctcca gcgcgcggtc
     2161 tgtgaccagc gcttcggacg cagcggggtc gaggcgctcg aggatctcgg ggtactcggg
     2221 ccgaactgct cgctgcccca ctcggtctgg atcgacgacg aagacatcga gcggatcgcc
     2281 cgtaccggct cgatcgtggt ccacaacccc gccgcgaacc tcaggctcgg cagcggacgc
     2341 tgcccggtcg gccgcctgct cgagcgggag gcgacggtcg cgatcgggac cgacggctcc
     2401 gcctcttccg acaaccagaa cgtctgggag atggtcaagc tcggggcttt gatccacaac
     2461 gacgggcccc gctgggtttc cggagccgac tctctcacga tggcaaccgg ctccggctac
     2521 caggtctggt cgggcggtga ggcaggcctc tctctgggcg gcgagatcgc agtaggcgcg
     2581 gttgccgacc tgatcctgct gaaccgctcg accgacggcc tggccggggt ccaggtgctc
     2641 gagccgggcc tggcgctctc cgaatccggg cgttcggtca agcacgtgat cgtcgacgga
     2701 cgggtcgtcg ttcgcgacgg caacctggcc acggccgacg gcgcggcggt cagggaggcc
     2761 ctgagggagc aggttcagcg ccgcagccgc gaggtcgcct cgccgccg
//
LOCUS       DAOQKN010000252         2797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_25040_length_2797_cov_4.377826, whole genome shotgun sequence.
ACCESSION   DAOQKN010000252 DAOQKN010000000
VERSION     DAOQKN010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25040_length_2797_cov_4.377826"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="PLJ64_12015"
     CDS             <1..385
                     /locus_tag="PLJ64_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576296.1"
                     /note="has catalase and peroxidase activities; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="catalase-peroxidase"
                     /protein_id="HNC94252.1"
                     /translation="LLDRANLLGLTAPEMTVLVGGLRVLGANTGGSKHGVLTDSPETL
                     TNDFFVNLLSFETEWKPADEDSTTFEAPGQGWTGTRVDLVFGSNSELRALAEVYASED
                     AKDKFVTDFIFAWTKVMNADRFDLD"
     gene            complement(498..1763)
                     /locus_tag="PLJ64_12020"
     CDS             complement(498..1763)
                     /locus_tag="PLJ64_12020"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNC94253.1"
                     /translation="MSRPPVAVVGAGLAGLKCAGLLADAGHEVLVFEASDGVGGRVRT
                     DEVDGFLLDRGFQVLLAAYPEVPRTFDLEALDPGEFDPGALVRLNGRFSPLLDPLRRP
                     AKTIAAVSSPVIGIADKLRVARMRQELLGKEPAEVLSRPDRTALESLKGRGFSPNAIE
                     RFFRPFFGGVFIDPDLGTSSRLMEIFFRCFSAGPAVLPKDGMGQLAASMAARLPEGSL
                     QLGSPVREVRADGVLPTDGDWIEALAVVVATDERSASRLAGLPEPEPGRVTSCVYFDA
                     PAGEISGRTLYLSPRAAGPINELAVPSAVSAGYAPAGRSLVSVSAIGENARRDDLEDA
                     VSAQLGEWFGAATVAGWRHLGTRTVEDALPDFGPGRFRAGGQDPALDSGLFVCGDYRE
                     SPSIQGALVSGRKAAAAVRAKLARARTEG"
     gene            complement(1792..2226)
                     /locus_tag="PLJ64_12025"
     CDS             complement(1792..2226)
                     /locus_tag="PLJ64_12025"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNC94254.1"
                     /translation="MASYSTSVESPKPVEEAFDYMADFRNAEEWDENTSSVKLTSGQA
                     GQAGSEYEVITGFAGRDMKLSYRTVEVDRPSKVVFESSTGMAKIRDTIEFSGDEKGCE
                     VKYDARILTNGLAKVIDPLFSLIFKRVGDRAAASLRKALGAR"
     gene            complement(2285..>2797)
                     /locus_tag="PLJ64_12030"
     CDS             complement(2285..>2797)
                     /locus_tag="PLJ64_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94255.1"
                     /translation="QSLLSGGDPRSLGRVDEVITTVLDDPGSCRELLDCLYGEDEIVR
                     MRAADGLEKVAATQPGIVEPFLPELLGPVARIDQPSVKWHLAQIFAEVELGEADRRRA
                     TALVEGYLEDSRDWIVITCSMTTLTGFAASDAELRERLVPVLRLHLDSDRKAIAKRAS
                     KMLAELGETS"
BASE COUNT          501 a          955 c          887 g          454 t
ORIGIN      
        1 gctgctcgat cgggccaacc tgctcggtct gaccgcgccc gagatgaccg ttctggtcgg
       61 cggcctgcgg gtgctggggg ccaacaccgg cggctcgaag cacggcgtcc tgaccgacag
      121 cccggaaacc ctcacgaatg acttcttcgt gaacctcctc tccttcgaga cggaatggaa
      181 gccggccgac gaggactcga ccaccttcga ggcccccggc cagggctgga ccggcacccg
      241 ggttgacctg gtcttcggct cgaactcgga actccgcgcc ctggccgagg tctacgcctc
      301 cgaggacgcg aaggacaagt tcgtgaccga cttcatcttc gcctggacga aggtgatgaa
      361 cgcggaccgc ttcgacctcg actgatcaac cgcagggatc gagatggcag aagtggccca
      421 ttatcgcccc aaactgccat ctcgatttgc accgaccgag cgcagaaacc tccggcaaag
      481 aggtttctgc gcgaggttca gccttcggtc ctggcgcgag ccagctttgc ccgcaccgcc
      541 gcggccgcct tgcggcccga gacaagagct ccttggatcg agggcgactc gcggtaatcc
      601 ccgcagacga aaagccccga atcaagtgcc ggatcctggc cgccggcccg gaagcggccc
      661 ggcccgaagt cgggcagcgc gtcctcgacg gtgcgagtcc cgaggtggcg ccagccggct
      721 accgtggcag ctccaaacca ctcgcctagc tgggcgctca cagcatcctc gaggtcgtca
      781 cgccgggcgt tttccccgat cgcgctgacc gaaaccagcg accttcctgc cggggcataa
      841 cccgcgctga ccgcactggg aacggcgagc tcgttgatcg gccctgcggc cctcggcgag
      901 agataaaggg ttctgccgct tatctcgcca gcgggagcgt cgaagtagac acaactcgtc
      961 accctgcccg gttcgggctc gggcaggccg gctaatctcg aagcggaacg ctcgtcggtc
     1021 gcgactacca ccgctagcgc ttcgatccag tccccgtcgg tgggcaggac gccatcggcc
     1081 cgaacctcgc gaaccgggga gccgagctga agcgagcctt ccggaagccg cgcggccatg
     1141 ctggcggcca gctgccccat gccgtccttc ggcaggaccg cgggaccggc cgaaaagcag
     1201 cggaagaaga tctccatgag gcgcgatgaa gtccccaggt ccgggtcgat gaacactccg
     1261 ccgaaaaagg ggcgaaagaa cctttcgatc gcgttcggtg aaaagcccct gcccttcagg
     1321 gattcgagtg cggtccggtc aggacgggaa agaacctcgg ctggctcttt gcctagcagc
     1381 tcctgacgca tgcgggcaac gcgcagcttg tcggcaatgc cgatcaccgg cgaactcacc
     1441 gcggcgatcg tcttggcggg gcggcggagg ggatcaagca gtggcgaaaa ccttccattt
     1501 agccggacca gcgcccccgg atcgaattca cccgggtcaa gggcctcgag atcaaaggtc
     1561 cggggaacct ccgggtaagc ggcaagcagc acctggaagc cgcggtcgag cagaaagcca
     1621 tcgacctcgt cggtgcggac ccggcccccg accccgtctg aggcttcgaa cacgagaact
     1681 tcatggccgg catcggcgag aagcccggcg catttcaggc cggcgagccc ggccccgacg
     1741 acggctaccg gcggcctgct caccggcgcc cggctccttg cgcgggcatg ctcaacgcgc
     1801 gcccagggcc ttccgcagac tggcggcagc ccggtcgccg acccgcttga agatcagcga
     1861 gaagaggggg tcaatcacct tggccaagcc gttggtcagg atgcgggcgt cgtacttcac
     1921 ctcgcagcct ttttcgtctc cactgaactc gatcgtgtcg cgaatcttcg ccatgccggt
     1981 cgaagattcg aagacgacct tcgagggacg atccacctcc accgtccggt aggagagctt
     2041 catgtcccgg ccggcgaagc cggtgatcac ctcatattcg gaaccggcct ggccggcctg
     2101 gccggaggtc agtttcaccg atgaggtgtt ttcgtcccat tcctccgcat tgcggaaatc
     2161 cgccatgtag tcgaacgctt cttcgaccgg cttcgggctt tctacggatg ttgagtagct
     2221 ggccacgggg aaagtctttc gcatccgcct caccggcgtg gaagcggagc ccggccgctg
     2281 agcctcaact ggtttcgcca agctccgcca gcatctttga ggcccgtttg gcgatcgcct
     2341 tgcggtccga gtccaggtgc aggcgcaaga cgggcaccag ccgctcacgg agttccgcgt
     2401 cactggccgc gaaaccggtc agggtcgtca tcgagcaggt gatcacgatc cagtcgcggg
     2461 aatcttcgag gtagccctcg acgagcgccg tcgcccgccg ccggtccgcc tctccgagtt
     2521 ccacttcagc gaagatctgg gccaggtgcc acttgactga tggctggtcg atccgggcca
     2581 ccggcccgag cagctccggc aggaagggtt cgacgatccc gggctgtgtg gccgccacct
     2641 tctcgagccc gtcggcggct cgcattcgca cgatctcgtc ttcgccgtaa aggcaatcga
     2701 gaagttcgcg gcaggaaccg ggatcatcga ggaccgtcgt gatcacctcg tcgacccggc
     2761 ccagagaccg gggatctccc ccgctcagaa gcgactg
//
LOCUS       DAOQKN010000253         2789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_25183_length_2789_cov_5.215801, whole genome shotgun sequence.
ACCESSION   DAOQKN010000253 DAOQKN010000000
VERSION     DAOQKN010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25183_length_2789_cov_5.215801"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="PLJ64_12035"
     CDS             complement(<1..346)
                     /locus_tag="PLJ64_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNC94256.1"
                     /translation="MSQSPEERPDLTELRRRHELTLDEARREAVARRHQRGGRTAREN
                     LEDLIDPGSFVEYGRYAIAAQRARFDTDDLISRTPGDGLIAGTATVNGELFGENSRCA
                     VLSYDYTVLAGTQ"
     gene            496..1161
                     /locus_tag="PLJ64_12040"
     CDS             496..1161
                     /locus_tag="PLJ64_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94257.1"
                     /translation="MGLAAVVAAFFVLAGTAYAGAVIQTDPVEYTFLPGPYVQGLGET
                     ATLDNSVSENWHDVVATKKGPDGKPLFSTPLARGGETKVVQGTQYLAAGTYHFICSFH
                     GAGMSGDLTIDGSSGSASPRPSVKLTFPAQSLKQVRKSGVKVKIKATTASSKVKVTAK
                     AGKVTLGTSRALDFTAGQSKTVNLPLTKAGRKAIARPKSVQIKLSAAVPYGKSASASR
                     KVK"
     gene            1166..>2789
                     /locus_tag="PLJ64_12045"
     CDS             1166..>2789
                     /locus_tag="PLJ64_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94258.1"
                     /translation="MSFRRFFVAFLMLAAILVLPQSASALLPHSTTKRLDDGTERITY
                     RIGPLTVTPGQNRIAFKPLTGIEKPSVDGWITRIKPNMVNEDGSIPLSSKVMFHHGVW
                     LNFSRQDATSGGQERFFATGEEKTITRFPDGYGYQYKASDVWLLNHMIHNLTPEAMTL
                     YATYTIDFIPADSPAADGIKPVTPIWMDVDNGSVYPVFDIWKGSGGKDGKFTYPDDAD
                     NPYPNGVAKNTWTVDRDGVLLATAGHVHTGGLYTDLYLQRPGARYAGPKCVQPKAARP
                     PNRRNIRRYGAKLKKYKSCKASMPSVKGSKVHLFRSQAHYFEPAGPVSWDMAMVGTRD
                     DWRPEVKKGDVLSMNSTYETKLASWPESMGIMVVYMAEGEHGTNPYRKKVDYKGQLIH
                     GHYAENNDHGGKLPVVGPDPTRLPDGANPDGNPFTISGFSFNGGDFRLPGAAGRPPVV
                     DEGKSLTFQLNQDDINDEIWHSLTSCKTPCNKSTGIAYPIPDGKFTFDSGQLGVGGAP
                     TVERTSWKTPADLPRGTYTFFCRIHPLMRGAFR"
BASE COUNT          532 a          945 c          863 g          449 t
ORIGIN      
        1 cctgggtgcc ggccagcacc gtgtagtcgt aggagagtac ggcgcatcga gagttctcgc
       61 cgaagagttc gccgttgacc gtggcggtgc cggcgatcag tccgtcgccg ggcgtgcgac
      121 tgatcaggtc atcggtgtcg aagcgggccc gctgggcggc gatcgcgtac cggccgtact
      181 cgacgaagct gccggggtcg atcaggtcct cgaggttctc gcgggcggtg cgaccgccgc
      241 gctggtgacg ccgggctacg gcttcccggc gggcctcgtc aagggtgagc tcgtgccggc
      301 gcctgagctc ggtcagatcg ggccgttctt cggggctctg cgacattccc gaaaactgtc
      361 acatcccggc gggcgacgcg ccgaagccgt agagccgagc gccagattcc gggccgtcgc
      421 ggcatttacc gttgcaagcg gagttgatat gcgtcataat ggagcgctgt gggagggatc
      481 agttccagaa ttgcgttggg gctggcggcc gtcgttgccg cctttttcgt gctggccgga
      541 accgcatatg cgggtgccgt gatccagacc gatccggtcg agtacacgtt cctgccgggt
      601 ccctacgttc agggtctggg cgagactgcg actctggaca actcggtttc ggagaactgg
      661 cacgacgtcg tcgcgacgaa gaaaggcccc gatggcaagc cgctcttctc gactcccctg
      721 gcccggggcg gcgagaccaa ggtggtccag ggcacccagt acctcgcagc cggcacctac
      781 cacttcatct gctccttcca cggggccggc atgagcggcg atctgaccat cgacggttcg
      841 agcggcagcg cttcgccccg cccctcggtc aagctcacct tcccggccca gagcctgaag
      901 caggtccgca agtccggtgt caaggtcaag atcaaggcga ccacggcgag cagcaaggtc
      961 aaggtcaccg cgaaggccgg caaggtcacg ctcggtacca gccgcgccct cgatttcacc
     1021 gcaggacagt cgaagacggt caacctgccg ttgaccaagg ccggccgcaa ggcaatcgcg
     1081 aggccgaagt ctgtccagat caaactgagc gccgccgtgc cctacggcaa gtcggcgagc
     1141 gcctcacgaa aggtcaagta gccagatgag cttccgcagg ttcttcgtcg catttctgat
     1201 gctcgccgcg attctggtcc tgccccagtc ggcttcggca ctgcttcctc acagcaccac
     1261 caaacgtctc gatgacggca ccgaacgaat cacctaccgg atcggcccgc ttaccgttac
     1321 gcccggccag aacaggatcg ccttcaagcc gctgaccggg atcgagaagc cctcggtgga
     1381 cggctggatc acccggatca agccgaacat ggtcaacgag gacggctcga tcccgctttc
     1441 gagcaaggtc atgttccacc acggtgtgtg gctcaatttc agccgccagg acgcaacctc
     1501 gggtggccag gaacgcttct tcgcaaccgg tgaggaaaag acgatcaccc ggttccccga
     1561 cggttacggc taccagtaca aggcatccga cgtctggctc ctcaatcaca tgatccacaa
     1621 cctgacgccg gaagcgatga cgctctacgc gacctacacg atcgacttca tcccggctga
     1681 ttcaccggcc gccgacggga tcaagccggt caccccgatc tggatggacg tggacaacgg
     1741 cagcgtctac ccggtcttcg acatctggaa gggcagcggc ggcaaggacg gcaagttcac
     1801 ctacccggat gacgccgaca atccctaccc gaacggcgta gccaaaaaca cctggaccgt
     1861 tgatcgcgac ggcgtcctgc tcgccaccgc cggccacgtc cacaccggtg ggctctacac
     1921 cgacctctac ctgcagcgcc ccggcgccag gtacgccggt ccgaagtgcg tgcagcccaa
     1981 agcggccaga ccgccgaacc gcaggaacat ccgtcgttac ggggcgaagc tgaagaagta
     2041 caaatcctgc aaggcttcga tgccgagcgt caagggcagc aaggtccacc tcttccgttc
     2101 gcaggcgcat tacttcgagc cggccggtcc cgtttcctgg gacatggcga tggtcggcac
     2161 cagggacgac tggcggcccg aggtcaagaa gggcgacgtc ctctcgatga actccaccta
     2221 cgagacgaag ctggcctcct ggcccgagtc gatggggatc atggtcgtct acatggccga
     2281 gggcgagcac ggcaccaacc cgtatcggaa gaaggtcgac tacaagggcc agctgatcca
     2341 cggtcactat gcggagaaca acgaccacgg cggcaagctt cccgtggtcg gacccgaccc
     2401 gaccaggctg ccggacggag ccaaccccga cgggaacccg ttcacgatca gcggcttctc
     2461 cttcaacggc ggtgacttcc ggctgcccgg cgcggccggt cgcccgccgg tggtggatga
     2521 aggcaagagc ctgaccttcc agctcaacca ggacgacata aacgacgaga tctggcactc
     2581 gctgacctcc tgcaagacgc cctgtaacaa gtcgaccggt atcgcctacc cgatcccgga
     2641 cggcaagttc accttcgact ccggccagct cggagtcggc ggagcgccca cggtcgagcg
     2701 gacctcatgg aagaccccgg ccgacctgcc ccgcggcacc tacaccttct tctgccgcat
     2761 ccacccgctg atgcgcggcg ccttccggg
//
LOCUS       DAOQKN010000254         2779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_25336_length_2779_cov_6.093979, whole genome shotgun sequence.
ACCESSION   DAOQKN010000254 DAOQKN010000000
VERSION     DAOQKN010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25336_length_2779_cov_6.093979"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PLJ64_12050"
     CDS             <1..262
                     /locus_tag="PLJ64_12050"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HNC94259.1"
                     /translation="PNAMVSSIDSAFDMSLNMASSTNYTLTVMTVVAGLLVPVVLLYQ
                     GWTYWVFRHRLSAEGFGDVKSPMDLLDQKKEAGSGPESDQRA"
     gene            283..1992
                     /gene="cydD"
                     /locus_tag="PLJ64_12055"
     CDS             283..1992
                     /gene="cydD"
                     /locus_tag="PLJ64_12055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydD"
                     /protein_id="HNC94260.1"
                     /translation="MATPSRAPGASRQARETQRRLARSSRVARNHLIVTVGLGFLQAV
                     LIIAQATLLAKVIAGVFMNGDSFAEVAPWLAWLAAISVGRGLAAAGFESAGRFGAARV
                     MSELREKLARHLLMNRPGALQAEQSGELVSSAIEGVAALENYFARYLPQMVLAGIVPV
                     AVLAWVLRYNWEAAAILAVTAPLIIVFMVLIGLLTEQRTRKRWERMSRLSSHFLDLVG
                     GLATLRANSQAYSQSGSIQAVGEDYRRETMGALRVGFLSSLVLELLAMIGVAMVATAV
                     GIQLAGGHLELVAGLTVLLLAPELYMPLRQLGAQFHASADGMAAAERIFEVIDQPAVV
                     STPAEPVPAPDPARWSVAMEGVSYAWPGRERVLESIDLELKPGETVALVGPSGGGKST
                     LASLLLRLTEPGSGRISCGGTDLSEVDPQSWRRQIAWVPQRPTVFADTLAANLRLFNP
                     QATDAQLAEAIGVAVLGEVVRGLPDGLETMVGEGGRRLSAGQTQRIALARALLSEAPL
                     LILDEPTAHLDQDTEKELAEAINEAASGRTALIISHREQPIARADRTLLLNQGRLGEM
                     VNA"
     gene            1989..>2779
                     /locus_tag="PLJ64_12060"
     CDS             1989..>2779
                     /locus_tag="PLJ64_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94261.1"
                     /translation="MIAARLERTPLGRAALATRNRWKLFTISVLLGTGTVLAAVGLLT
                     ASGYLISRAAERPEILSLTVAIVGVRFFGVSRALLRYGERLTSHDLAFRTLTDLRGRF
                     FERLIPLVPAGLDGSRRADLMSRFVGDVDRLQDLYLRALSPPLVAIFCSVFCVALASI
                     ILPVAGLVLAVMLLLGGLAAPALTRWAARTAGRRQSRARGLLAMDLHEIAVGGAEIAV
                     AGRESDWLERSREDDRHLVSLQRRDAMSGGLAEGLGTLLAVGAAVA"
BASE COUNT          402 a          914 c          974 g          489 t
ORIGIN      
        1 cccgaacgcg atggtttcaa gtatcgattc ggccttcgac atgagcctga acatggccag
       61 ctcaaccaac tacacgctga ccgtcatgac ggtcgtcgca gggttgctgg tcccggtggt
      121 tctgctctac cagggctgga cctactgggt cttccggcac cgtctttcgg ccgaagggtt
      181 cggtgacgtg aagtcaccga tggacttgct tgaccagaag aaggaggccg gaagtggccc
      241 ggagtcggac cagagggcgt agccggggcc gaggggagag tattggccac gccctctagg
      301 gcaccgggag cgagccgtca ggctcgtgaa acacagcgcc ggctggcccg gtccagccgt
      361 gtagctcgca atcacctgat cgtgacggtc ggcctggggt ttctccaggc cgtcctgatc
      421 atcgcccagg cgaccctgct ggccaaggtg atcgccgggg tcttcatgaa cggggactcc
      481 ttcgccgagg tcgccccgtg gctggcctgg ctggccgcga tctcggtcgg ccggggtctc
      541 gccgcggccg gatttgaaag tgcgggccgt ttcggcgcgg cccgggtcat gtccgagctc
      601 cgggagaagc tggcccgcca cctgctgatg aaccggccgg gtgccctcca ggccgaacag
      661 agcggtgaac tcgtcagctc ggcgatcgaa ggggtcgccg cgctggagaa ctacttcgcc
      721 cgctacctgc cgcagatggt gctggccggg atcgtgcccg tagccgtgct ggcctgggtg
      781 ctccgttaca actgggaagc ggcggcgatc ctcgcggtta cggcgccgct gatcatcgtc
      841 ttcatggtcc tgatcggact gctgaccgag cagcgcaccc gcaagcgctg ggagcggatg
      901 tcccggctgt cgtcgcattt cctggacctg gtcggcgggc tggcgacctt gcgggccaac
      961 agccaggcct actcgcaatc gggctcgatc caggcggtcg gggaggacta tcgccgggag
     1021 accatggggg ctctccgggt cggcttcctt tcctcgctgg tccttgaact tctggcgatg
     1081 atcggggtgg cgatggtcgc gaccgcggtc ggaatccagc tcgccggcgg ccatcttgaa
     1141 ctggtcgccg ggctgaccgt gctgctgctc gcccccgagc tttacatgcc cctgcgccag
     1201 ctcggcgcgc agtttcacgc cagtgccgac ggcatggcgg cagccgagcg aattttcgag
     1261 gtgatcgacc agccggcagt ggtgtccacg ccggctgaac cagtacccgc tcccgacccg
     1321 gcccgctggt cggttgccat ggagggcgtt tcatacgcgt ggccgggtcg ggagcgggtg
     1381 ctggagtcga tcgacctcga actgaagccc ggcgaaacgg tggcgctggt cggcccgagc
     1441 ggtggcggca agagcaccct ggcttcgctc cttctgcggc tgactgaacc gggttccggc
     1501 cggatcagct gtggcggcac tgacctgtct gaagtcgatc cgcagagctg gcggcggcag
     1561 atcgcctggg tgccccaaag gccgaccgtc ttcgccgaca cactggcggc gaacctgagg
     1621 ttgttcaatc cgcaggccac cgacgcccag ctggccgaag caatcggggt ggcggtgctg
     1681 ggtgaagtgg tcagggggct gcccgacggc ctggaaacca tggtcggcga aggaggccgg
     1741 cggctgtcgg ctggtcaaac ccagagaatc gccctcgcca gggccctttt gagcgaggcc
     1801 cctctgctga tcctcgatga accgaccgct cacctcgacc aggacaccga gaaggaactc
     1861 gcggaagcga taaatgaagc cgcgagtggc cggaccgccc tgatcatctc ccatcgcgag
     1921 cagccgatcg cgagggccga ccggaccctg ctgctgaacc aggggcgact gggtgaaatg
     1981 gtgaacgcat gatcgccgcc cggctcgaac ggaccccgct gggccgagcc gccctcgcga
     2041 ccaggaatcg ctggaagctg ttcacgattt cggtgctgct cggtacgggc acggtgctgg
     2101 cagcggtggg tctgctgacc gcctccggct acctgatcag ccgtgccgcc gagcggccgg
     2161 agatcctctc gctcaccgtg gcgatcgtcg gggtccgctt cttcggggtc tcccgggccc
     2221 tgcttcgcta cggcgagcgc ctgacctcgc atgacctggc cttcagaacc ctcaccgacc
     2281 tgcgcggccg gttcttcgaa cgcctgatcc cgctggtccc ggccgggctc gacggctcgc
     2341 ggcgggccga tctgatgagc cgtttcgtcg gtgacgtcga ccgcctccag gatctgtatc
     2401 tgcgggccct ttcgccgccg ctggtcgcga tcttctgctc ggtcttctgc gtggcgctcg
     2461 cctcgatcat cctgccggtg gccgggctgg tgctggccgt gatgctgctg ctcggtggtc
     2521 tcgcggctcc ggccctgacc cgctgggcgg cacggacggc cggccgccgc cagtccaggg
     2581 caaggggcct tctggccatg gaccttcatg agattgccgt cggcggagcc gagatcgccg
     2641 tggccggtcg cgaaagcgac tggctcgaga gaagccggga ggatgaccgc cacctcgttt
     2701 cactccagcg gcgggacgcg atgagcggcg gtctggcgga aggtctcgga accctgctgg
     2761 ccgtaggcgc cgcggttgc
//
LOCUS       DAOQKN010000255         2764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_25599_length_2764_cov_5.812108, whole genome shotgun sequence.
ACCESSION   DAOQKN010000255 DAOQKN010000000
VERSION     DAOQKN010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25599_length_2764_cov_5.812108"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="PLJ64_12065"
     CDS             <1..243
                     /locus_tag="PLJ64_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94262.1"
                     /translation="PAYGMETGMTIDAVRAGYRLGEYEIDLAHKATGKTFKGFIHRFR
                     QLLDFARVWWARRGGRGPDGKRPVESVAGSRPGATG"
     gene            240..1745
                     /gene="glpK"
                     /locus_tag="PLJ64_12070"
     CDS             240..1745
                     /gene="glpK"
                     /locus_tag="PLJ64_12070"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HNC94263.1"
                     /translation="MILAIDQGTTGSTALVFNDEGRICGRGYAEFRQHFPKPGWVEHD
                     AEEIWETSWGVAARALAGAGISGPDLDAIGITNQRETVVAWDRETGKPLHHALVWQDR
                     RGAARCDELKAVGLEGLVRERTGLVLDPYFSATKIEWLRQNVEGADRAVFGTIDSWLV
                     HRMCGEHLTDLTNASRTMLFDTGRLQWDPELCEVFGTDPASLPEARPSAGMFGTTSGF
                     GGEVPVAGIAGDQQAALFGQMCHEPGMAKNTYGTGSFVLLNAGDEKPDPGEGLLGTIA
                     WGIGNGAGSGAENKEVSVEYALEASVFVTGSAVQWLRDGLGIIKDASETEALAASLDS
                     NDGVYFVPALTGLGSPWWDPYARGTIVGLTRGTGRAHFARAALEAIAYQTVDAVRAQE
                     SASGEKLEALRADGGAVANKWLMQFQADLLGVPVEIPEISETTAFGAACLAGIGSGSW
                     TVEQVRGMWKPTARYEPKMNRDEAESLLNDWHRALESTLTASRAGSDPPSQ"
     gene            1834..2607
                     /locus_tag="PLJ64_12075"
     CDS             1834..2607
                     /locus_tag="PLJ64_12075"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNC94264.1"
                     /translation="MSELKPQKYKDNRPAEYFDHIHERSRNHEPNWVYEAVRAALTPI
                     AVGLYRTRAIGTENVPAEGPVLLAPNHFSNMDHFFAGVYLRRKIRFMAKSQLFGAPVL
                     TQIFKYGGVFPVRRGYKDEEAFKTVDAIFDQGGCVLVYAEGGRSRTGGLGEAKPGIGR
                     IALDSGVPVVPVAIHGSASVRKWKRLVLPKVTVQFGEPMTFPVIENATREQQLDCSKE
                     VFDQVKKMYTALDEKGRRGVKQALREKVKAPEPGRTIVP"
BASE COUNT          497 a          870 c          972 g          425 t
ORIGIN      
        1 cccgcctacg gaatggagac cggaatgacc atcgacgccg ttcgggccgg ctaccggctc
       61 ggcgagtacg aaatcgacct cgcccacaag gccaccggca agaccttcaa gggattcatc
      121 caccggttcc gccagctcct cgatttcgcc cgggtctggt gggccaggcg cggtggccgc
      181 ggaccggacg ggaagcgacc ggttgaaagt gtcgccggga gcaggcccgg agcgacagga
      241 tgatcctcgc gatcgatcag gggaccaccg gttcgaccgc gctggtcttc aacgacgagg
      301 gccggatctg cggtcgcggc tacgccgaat tccggcagca cttcccaaag ccgggttggg
      361 tcgagcacga tgcggaggag atctgggaga ccagctgggg ggtggcggca cgggctctgg
      421 ccggggccgg gatcagcggt cccgacctcg acgcgatcgg gatcaccaac cagcgcgaga
      481 cggtggttgc ctgggaccgg gagaccggca agccgctgca tcacgccctg gtctggcagg
      541 accgccgggg cgctgcccgc tgcgatgagc tgaaggcggt ggggctggag ggactggttc
      601 gcgaacggac cgggctggtc ctcgaccctt atttctcggc gaccaagatc gagtggctgc
      661 gccagaacgt cgaaggcgcg gaccgggcgg tcttcggaac gatcgactcc tggctggtcc
      721 accggatgtg tggcgagcac ctgaccgacc tgaccaacgc ctcgcggacc atgctgttcg
      781 ataccggcag gctccagtgg gacccggagc tctgcgaggt attcgggacc gaccctgcct
      841 cgctgcccga ggcccggccc tcggcgggaa tgtttggcac gacctcgggc ttcggcggtg
      901 aggtgccggt ggccgggatc gccggtgacc agcaggctgc cctgttcggg cagatgtgtc
      961 acgagccggg catggcgaag aacacctacg gcaccggcag cttcgtactg ctcaacgccg
     1021 gcgacgagaa gccggatccg ggggagggct tgctcggaac gatcgcctgg gggataggca
     1081 acggcgccgg ttccggtgcc gaaaacaagg aggtctcggt cgaatacgcc ctcgaagcct
     1141 ctgtcttcgt gaccgggtcg gcggtccagt ggctgcggga cggcctcgga atcatcaagg
     1201 acgccagcga gaccgaagcc ctggcggcgt cgctcgactc gaacgacggc gtctatttcg
     1261 taccggccct gaccggactc ggatctccct ggtgggatcc atacgcccgc ggcacgatcg
     1321 tcgggctgac ccgcggcacc ggtcgtgccc atttcgcccg ggcggccctc gaggcgatcg
     1381 cctaccagac ggtggatgcg gtccgggccc aggaatcggc atccggcgag aagctcgaag
     1441 cgcttcgggc cgacggcggc gcggtggcca acaagtggct gatgcagttt caggccgacc
     1501 tgctcggagt cccggtcgag attcccgaaa tcagcgagac caccgccttc ggcgcggcct
     1561 gcctggccgg gatcggcagc ggcagctgga cggtcgaaca ggttcgcggt atgtggaagc
     1621 caaccgcccg ctacgagccg aagatgaacc gcgacgaggc ggaaagcctc ctgaacgact
     1681 ggcatagagc cctagagagc accttaactg caagtcgggc ggggtccgac ccgccctccc
     1741 agtgagaacc cgccgaccca cagaaaaaat gacggatggg tcggctccgt cgggtgtaac
     1801 cccgggcagg cctttgggta gaccggctaa ctcatgtccg aactgaagcc tcagaagtac
     1861 aaagacaacc ggcccgccga gtatttcgac cacatccacg aacggtcgag aaaccacgag
     1921 ccgaactggg tctacgaggc ggtcagggcg gcactgaccc cgatcgcggt cggcctgtac
     1981 cggacccggg cgatcggaac cgagaacgtc ccggccgaag gcccggtact gctggcgccc
     2041 aaccactttt cgaacatgga ccacttcttc gccggggtct acctgcgccg gaagatccgc
     2101 ttcatggcga agtcacaact tttcggggcc ccggtactga cccagatctt caagtacggc
     2161 ggagtgttcc ccgtgcggcg cggctacaag gacgaagagg cattcaagac agtggatgcg
     2221 atcttcgacc aggggggttg cgtgctggtc tacgccgagg gcggccggtc ccggaccggc
     2281 ggtctcggtg aggcgaagcc cggcatcggt cggatcgccc tcgacagcgg ggttccggtt
     2341 gtacccgtag cgatccacgg ttcggcgtcg gtccgtaaat ggaagcgact ggtgttgccg
     2401 aaggtgaccg tccagttcgg cgaaccgatg actttcccgg tgatcgagaa cgcaacccgg
     2461 gagcagcagc tcgactgctc gaaggaagtc ttcgatcagg tcaagaagat gtacacggcc
     2521 ctcgacgaga aggggcgacg cggcgtgaag caggcgttgc gcgagaaggt gaaggcgccg
     2581 gagcctggcc ggacgattgt gccctgagcc ttgggggcgg ggccggtggg gcctcctcct
     2641 cggcccgaaa cgtcgtcgga gtccccaccg gccccgcctg acgtctcggg ataactactc
     2701 ccagacggcc ggggcgtttt cgtagaaggc tgtttcgtgg ccggcggtta ttacggctga
     2761 gggg
//
LOCUS       DAOQKN010000256         2755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_25745_length_2755_cov_3.153333, whole genome shotgun sequence.
ACCESSION   DAOQKN010000256 DAOQKN010000000
VERSION     DAOQKN010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25745_length_2755_cov_3.153333"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1594
                     /locus_tag="PLJ64_12080"
     CDS             <1..1594
                     /locus_tag="PLJ64_12080"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNC94265.1"
                     /translation="VDSSRVDFAVASGADHGLLVNGETIASVESLTGARGVDRVIVAA
                     ASGNPAPLSLGAAIARDRGSLILVGAVPIEFERAPLFEKELEFKVSRSYGPGRYDTEY
                     EEHGLDYPFGYVRWTEQRNLEAVLGLLERGDLFLDDLVEEIVPVEQAEAAYAKLVAAD
                     PDARPLGAIVLSYGDPADGIQAPEREIGEPQAEPAPIVPTAGPPRIGLVGPGGFASRI
                     LLPAFETAGSDFELVGGGNGPSAEAAVREKGFARVAETPEAVCSDPDVDAVVIATRHG
                     SHAELSRAALLAGKHTFCEKPLALSTDELDGVIEAAEGSKGVLAVGFNRRFAPMVREA
                     RAHISAVTGPVTATFRVSAGELPPDHWTQDLEQGGGRLLGEGCHFIDTLRYLVGHRIE
                     SVHAIAHGNPDTPVQAQDCLLVSLTFADGSIGQLTYVSQGSPKVGKERLEIFGGGGRS
                     AIMDNYKTLELYDGPKVSRTTTKSPDKGHKGEVSAFVDAARGKPGSAPVPLAEVANVS
                     YATLAAVRSLETGGPVRLALIE"
     gene            1597..>2755
                     /locus_tag="PLJ64_12085"
     CDS             1597..>2755
                     /locus_tag="PLJ64_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94266.1"
                     /translation="MKSKLQELLRAGRSGIGRQTLAFLAFSAVGSLLTAAITALLVRE
                     LSTDDFGAFTLSKSLLLLVASIFEFGIFYPAARLTALSETKVEQRKWMGAMLIAYIPF
                     GAVYVATIMFLSMFVDSVFNVNASHALLVSAPFAFAYPFVLVVESIGRGTGRAYLPPL
                     MSAIAGVIMLAGLAAFIFIEDSLPLGFVLALRGGSFIAGGVAVALLVRPLFADLRSRW
                     NRMWSETRSWGAKVYVGSLLAMATYNLDVLIVGALADTQQVASYGLAVAGASLIGLPA
                     IALATALYSRMARLDKLDRRPVELLVLWGMTATVVGAVFAAIVIPKYLNPELEEAAIL
                     VIPLGLAATVGSINTLISFYLSARASGSEMRNAALVLFISNITLTPLLVLGF"
BASE COUNT          462 a          892 c          849 g          552 t
ORIGIN      
        1 cgtggacagc tcgagggtgg acttcgccgt cgccagcgga gccgatcacg gacttctggt
       61 caatggtgaa accatcgcct ccgtcgaatc gctgacgggc gcccgcggtg tggaccgggt
      121 aatcgtcgcc gcggcctcag gcaaccctgc gccgctgtcc ctcggtgccg ccattgccag
      181 ggaccggggc tcgctgatcc tggtcggtgc cgtaccgatc gagttcgagc gcgctccgct
      241 cttcgaaaaa gaacttgagt tcaaggtctc gcgatcctac ggacccggtc gctatgacac
      301 cgaatacgag gagcacgggc tcgattatcc gttcggctac gttcgctgga ccgagcaacg
      361 caacctcgaa gccgtcctcg gactccttga gcggggtgac ctcttcctcg acgacctggt
      421 cgaagaaatc gttccggtcg agcaggcgga agctgcttac gcaaagctgg tcgccgcgga
      481 ccccgacgcc cgtccgctgg gagcgatcgt cctttcctac ggcgatccgg ccgacgggat
      541 tcaggctcca gaacgagaaa tcggtgagcc tcaggctgag cctgccccca tcgtgcccac
      601 cgccgggcca ccacgcatcg gcctggtcgg accgggtgga ttcgccagcc gtatccttct
      661 tcccgccttc gagacagcgg gttctgactt cgagctagtc gggggcggca atggcccttc
      721 ggccgaggcc gccgttcgcg aaaagggatt tgcgcgggtt gcggaaaccc ccgaggccgt
      781 ttgctccgat ccggacgtgg atgccgtggt catcgcgacc cgtcacggat cccacgccga
      841 gctgtcccgg gcggcgctgc tggccggcaa gcacaccttc tgcgagaaac ctctggcgct
      901 gagcaccgat gaactggatg gtgtcatcga agccgccgaa ggttcgaagg gtgttctggc
      961 ggtcggattc aaccggaggt tcgctccgat ggtccgcgaa gcccgcgccc acatttcggc
     1021 agtcaccgga cctgtcaccg ccactttccg ggtgagcgcc ggggagctcc ccccggatca
     1081 ctggacccag gaccttgagc agggcggagg ccgcctgctc ggcgagggat gtcacttcat
     1141 tgacactctc cggtacctgg tcggacaccg catcgagtcg gttcatgcga tcgcccacgg
     1201 caatcccgac acgcccgtcc aggctcagga ttgcctcctg gttagcctga ctttcgccga
     1261 cggttccatc ggccagctca cttatgtgtc ccagggatca ccgaaggttg gcaaggaacg
     1321 gctggagatc ttcgggggcg gaggaaggtc cgccatcatg gacaactaca agaccctcga
     1381 gctctatgac ggaccgaaag tcagccggac cacgaccaaa tccccggaca agggtcataa
     1441 gggcgaggtc tcggccttcg tggatgcggc tcgcgggaag cccggctcgg cgccggtgcc
     1501 actggctgag gtggctaacg tcagttacgc gaccctggcc gcggtcagga gcctcgaaac
     1561 gggtggaccc gtgcgtctgg ccctgatcga gtaggaatga agtcgaagct ccaggaactg
     1621 ctccgtgccg gtcgctccgg catcggacgc cagactctgg ctttccttgc gttttcggct
     1681 gtcggaagcc tcctgacggc ggcgatcacc gcgttgctcg tccgagaact ttcgacggac
     1741 gacttcggtg ccttcaccct cagcaagtca cttctgctgc tggtggcgag catcttcgag
     1801 ttcgggatct tctatcccgc cgcccggctc accgctctgt ccgagacaaa ggtggaacag
     1861 cggaaatgga tgggtgccat gctgatcgcg tatatcccgt ttggcgctgt ctacgttgcg
     1921 acgatcatgt ttctgtcgat gttcgtcgat tcggttttca acgtgaacgc gagccacgcg
     1981 ttgctggtga gcgcgccgtt cgcgttcgcg tatcccttcg tcctggtagt cgaatcaatc
     2041 ggtagaggaa ccggacgcgc ctacctccct ccgctgatgt cggccatcgc gggtgtaatc
     2101 atgctggccg gactcgccgc tttcatattc attgaagaca gtctccccct agggttcgtg
     2161 ctcgccctca gaggtggctc cttcatcgcg ggaggcgtag cggtcgctct gctggtcagg
     2221 cccttgttcg ccgaccttcg ttcgcgatgg aacaggatgt ggtcggaaac ccgtagctgg
     2281 ggcgccaagg tttacgtcgg atcacttctc gcgatggcca cgtacaacct ggacgtcctg
     2341 atcgtcggtg ccctggccga tacgcaacag gtggcctcct acggactggc ggtggccggg
     2401 gccagcttga tcggcttgcc ggccattgct ctcgcaacgg ccctctattc ccgcatggcc
     2461 cgtctcgaca aactcgatcg cagaccggtg gagcttcttg tcctgtgggg gatgacggca
     2521 accgtggtcg gagcggtctt cgccgcgatc gtcatcccca agtatctgaa cccagaactc
     2581 gaagaagccg cgatcctggt catccccctc ggacttgcag ccaccgtggg gtcgatcaac
     2641 accctgatca gtttctacct gagtgctcgg gcttccggca gcgaaatgag aaacgccgcc
     2701 ctcgtcctgt tcatttctaa catcaccctc actcccctgc tcgtactggg attcg
//
LOCUS       DAOQKN010000257         2745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_25917_length_2745_cov_3.765428, whole genome shotgun sequence.
ACCESSION   DAOQKN010000257 DAOQKN010000000
VERSION     DAOQKN010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25917_length_2745_cov_3.765428"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1236
                     /gene="glmS"
                     /locus_tag="PLJ64_12090"
     CDS             <1..1236
                     /gene="glmS"
                     /locus_tag="PLJ64_12090"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNC94267.1"
                     /translation="ETRLALTIDDGEIVTLTADGVTVTKADGEPVERATEEVEWDEET
                     AEKAGYDTFMMKEIHEQPEAIAETILDRLTEDDSVDLSDIGFGDEVFRQCSRIVIVAC
                     GTSYHAGLVGRYAIERWARVPVEMDIASEYRYRDPVIGEDDLVIGITQSGETADTLAA
                     MRLARDRGAKVLAITNIMGSQATRDSDAVLFTRAGLEIGVAATKTFVAQVAAMYLVAL
                     RLAELRGNLGFSEVSRLINELRAIPEKMEATIDSESERMLEIAKLHYDQKFFLYLGRH
                     IGLPVCLEGALKLKEVSYIPTDAYAAGEMKHGPIALLDESTPVVCVATDSPILDKVLS
                     NMEEVKARGADVIAVATEGDDRVGKVSEETISVPRTDWVLQPLVAILPLQLLAYHVAR
                     LNGLNVDQPRNLAKTVTVE"
     gene            1340..>2745
                     /locus_tag="PLJ64_12095"
     CDS             1340..>2745
                     /locus_tag="PLJ64_12095"
                     /inference="COORDINATES: protein motif:HMM:NF023260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3352 domain-containing protein"
                     /protein_id="HNC94268.1"
                     /translation="MSSHLKKFSVPTILATCLAAFAISACGSSSDGSGSTELAKYAPA
                     DVPVYVEGAVQPDSEVAANVDSISETLAGVNLGDMIKDGIESSSDGEVNFDDDVKPWL
                     GENAGVFVQFDPADLAPDSGLGSDGLSSLTSSETTLDGTAVADYSSSSEAFGLVVETT
                     DTDAAQSFIDKQASTDGGSTDGEYEGFSYKISKSDGTAVGIVDDNVVIGSSEAEFKAA
                     VDASKGDNLADTDAFGDLSGHVADGALATVFTNNDPYLAALKQQGFDLGGLYSALGIN
                     LDGSGTVISLVPESDEISMQGYSNAGSDLTSGDPTSLIETFPANTVFATGSGDVGPNA
                     TKIMNALNEEGIPGLLKPGQVDKFINEASGQIDIKGIIDSLETVAFFVNGTTEATLGG
                     ALVATSSDIKPIESSLRGISSLIGLAGDARVQPLPGGVAGFRVFTPELPGRPVVVGVK
                     GDRMVIGIGMKASLNALTG"
BASE COUNT          522 a          934 c          844 g          445 t
ORIGIN      
        1 gaaacccgcc tcgccctgac catcgatgat ggcgagatcg tcaccctgac cgctgacggc
       61 gtcaccgtga ccaaggccga tggcgagccg gtcgagcggg ctaccgaaga ggtcgagtgg
      121 gatgaggaga ccgccgagaa ggccggatac gacaccttca tgatgaagga gatccacgag
      181 cagccggaag cgattgctga gaccatactt gaccgtctga ccgaagacga cagtgtggat
      241 ctttccgata tcggtttcgg cgacgaggtc ttccgccagt gctcgcggat cgtgatcgtc
      301 gcctgcggca ccagctacca cgccggtctg gtcggtcgtt acgcgatcga gcgctgggcc
      361 cgggtgccgg tcgagatgga catcgcctcc gagtaccgct accgcgaccc ggtgatcggt
      421 gaagacgatc tggtcatcgg catcacccag tccggcgaga ccgccgacac cctggccgcg
      481 atgcggctgg cccgcgaccg cggcgccaag gtcctggcga tcaccaacat catgggcagc
      541 caggcgaccc gcgactcgga cgcggtcctc ttcacccgag ccggactgga gatcggggtc
      601 gccgcgacca agaccttcgt cgcccaggtg gccgcgatgt atctggtcgc ccttcggctg
      661 gccgagctgc gcggaaatct cggcttttcc gaggtgagcc gcctgatcaa cgaactgcgc
      721 gcgatcccgg agaagatgga ggcgacgatc gactccgaat ccgagcggat gctggagatc
      781 gcaaagctcc actacgacca gaagttcttc ctctatctcg gccgtcacat cggcctgccg
      841 gtctgcctcg aaggcgccct gaagctgaaa gaggtctcct acatcccgac cgacgcctac
      901 gcggcgggcg agatgaagca cggcccgatc gccctgcttg acgaatcgac cccggtcgtc
      961 tgcgtggcca ccgacagccc gatcctcgac aaggtcctct ccaacatgga agaggtcaag
     1021 gctcgcgggg cggacgtgat cgcggtcgcc accgaaggtg acgaccgggt cggaaaggtc
     1081 tcggaagaga ccatttccgt tccccgcacc gactgggtcc tccagcccct ggtcgcgatc
     1141 ctccccctcc agctactcgc ctaccacgtg gcccgactaa acggcctcaa cgtagaccag
     1201 ccccgaaacc tcgccaagac cgtaaccgtc gagtaacccg ccccgccgcg gccccacgac
     1261 cccacggccc ccgacccggg tccgggggtc gaccggggtt cgcaagaagt tcgcaagggt
     1321 cgtcggtagg atcgatgtca tgtccagtca tttgaagaag ttctcagttc caaccatcct
     1381 tgcaacctgt ctcgcagcct ttgcgatcag cgcctgcgga agcagttctg acggctcagg
     1441 ctcgaccgaa ctcgccaaat acgcgccggc cgacgtgccc gtgtacgtgg agggcgccgt
     1501 tcaaccggat agcgaagttg ccgccaacgt cgactcgatc tccgaaacgc tcgcgggtgt
     1561 gaacctcggc gacatgatca aggacgggat cgaaagctcc agcgacggtg aagtgaactt
     1621 cgacgatgac gtcaagccct ggcttggcga gaacgccggc gtcttcgtcc agttcgatcc
     1681 ggccgatctc gcacccgact ccgggcttgg cagcgacgga ctctcctcac tgacctcgtc
     1741 cgagactacg ctcgacggga ccgcggtggc ggactactcg agcagctccg aggccttcgg
     1801 gctggtggtc gaaaccaccg acaccgacgc ggcccagtcc ttcatcgaca agcaggcttc
     1861 aaccgacggc ggctccaccg acggcgagta cgaaggcttt tcgtacaaga tctccaagag
     1921 cgacgggacg gccgtcggca tcgtcgatga caacgtggtg atcggttcct ccgaagcgga
     1981 gttcaaggct gcggttgacg cctccaaggg tgacaacctc gccgacaccg atgctttcgg
     2041 ggacctctcc ggtcacgtcg ccgacggcgc cctggccacc gtcttcacca acaacgaccc
     2101 ctacctcgcc gcgctcaagc agcagggctt cgatcttggc ggcctctact cggcactcgg
     2161 gatcaacctc gacggcagtg gcaccgtgat cagcctcgta ccggaatccg acgagatctc
     2221 gatgcagggc tacagcaacg ccggatcgga tctgaccagc ggcgacccga cctcgctgat
     2281 cgagaccttc ccggccaaca ccgtcttcgc caccggctcc ggtgacgtcg gacccaacgc
     2341 gaccaagatc atgaatgcgc tgaacgagga aggcatcccg gggctgctca agccgggcca
     2401 ggtcgacaag ttcatcaatg aggcatcggg acagattgac atcaagggga tcatcgactc
     2461 gctcgaaacg gtcgccttct tcgtcaatgg cacgaccgag gcgaccctcg gcggcgccct
     2521 ggtcgcgacc agctccgaca tcaagccgat cgagagttcg ctgcgcggaa tctcctcgct
     2581 gatcgggctg gccggcgacg cgagggtcca gcccctgcct ggcggcgtgg ccggcttccg
     2641 ggtcttcacc ccggagctcc cgggccgccc cgtggtggtc ggtgtgaagg gcgaccgcat
     2701 ggtgatcggg atcggcatga aggcttcgct caacgccctg accgg
//
LOCUS       DAOQKN010000258         2702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_26635_length_2702_cov_6.834907, whole genome shotgun sequence.
ACCESSION   DAOQKN010000258 DAOQKN010000000
VERSION     DAOQKN010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26635_length_2702_cov_6.834907"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="PLJ64_12100"
     CDS             <1..411
                     /locus_tag="PLJ64_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid deaminase/aldolase"
                     /protein_id="HNC94269.1"
                     /translation="VAAGSGFFAPAQFDHYSNLSLRPAAGYALPVVRRPEPRVVTALG
                     GGYLASGPPDPGRMPVPWLPEGLEFEKDEGAGEVQTPLVGEAAQELAIGDNVYLRHAK
                     AGELCERFESLILIEGDEIVDEVPTYRGEGQTFL"
     gene            complement(434..1030)
                     /locus_tag="PLJ64_12105"
     CDS             complement(434..1030)
                     /locus_tag="PLJ64_12105"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC94270.1"
                     /translation="MNPVDAGLPAGVTESVEVPRRADAVRNREKVIAAAEQVFAEHGI
                     EAGVPEIAEKAGVGKGTVYRNFETKDDLVAAVLIRRMNRFDEDILDALESDDPGAAFG
                     EALRKAATRASDLSFQAGIYWSGSSEELIEVKARVKGHMARLVRKAQKAGAIRKDATV
                     DEVWILFGGICRALGDAGEKDPKVWRRHTDLVVDAFRP"
     gene            complement(1027..2505)
                     /locus_tag="PLJ64_12110"
     CDS             complement(1027..2505)
                     /locus_tag="PLJ64_12110"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC94271.1"
                     /translation="MSPFSQSSAGSDRPHPTRTLLILSLAALSFALAQTTLIPALAEL
                     MEEFHTDATGIAWVLTGYLISAAIFTPIFGRLGDMFGKRRLLVIALSIFAAGSTVSAL
                     GDSVPAIVAGRILQGVGGGIFPLCFGIIRDEFPPEKVSASVGLMSATLGIGGGIGLVV
                     GGLIVDHASYHWIFWLGAAMAVMAAAAALIFVPESPNRVPAKVDYRGAAVLAVGLSVP
                     MYAISEANNWGWTSTKTIGLILAGFVVITFWVWLQKRTREPLADVSLLAEPRVAMTNT
                     ATLLIGFGMFASFILIPQLVESPDSTGYGFGFSATQAGLVMLPAALMMLPAGPLSGHL
                     GARFGNRVPLALGGLISGIGLIGLGLDHGSALSIVLWNVLSSIGVGLAYAAMPNLIME
                     AVPLERTGEATGFNTLIRSVGSSLGSQVTASILTGTILAATGFPTSDGFRTAFLVGAG
                     VSIFAGLLALLIPRSGHRHAIFTEEIGAASVLPEPALAADER"
     gene            complement(2545..>2702)
                     /locus_tag="PLJ64_12115"
     CDS             complement(2545..>2702)
                     /locus_tag="PLJ64_12115"
                     /inference="COORDINATES: protein motif:HMM:NF015963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HNC94272.1"
                     /translation="DGRPHWGKLHFRTAEDLAPSFPRWADFQKVRDELDPNRNFSNAY
                     VTEVLGA"
BASE COUNT          465 a          884 c          949 g          404 t
ORIGIN      
        1 gtggcggccg ggtcgggctt cttcgcgccg gcccagttcg accactactc gaacctgagc
       61 ctgcgacccg ccgccggtta cgccctgccc gtggtcagga ggcccgagcc tcgagtggtc
      121 accgcgctcg gcggcggcta cctcgcttcg gggccgccgg acccgggccg gatgccggtc
      181 ccctggctgc cggaggggct cgagttcgaa aaggacgagg gcgccggcga ggtccagacc
      241 ccgctggtcg gggaggccgc ccaggagctc gcgatcggcg acaacgtcta cctccgccac
      301 gccaaggccg gagagctctg cgaaaggttc gagtcgctga tcctgatcga gggcgatgag
      361 atagtcgacg aggtccccac ctaccggggc gagggccaga ccttcctcta ggcgggaggc
      421 gccggaagtt cccctagggc cggaaggcgt cgaccaccag gtcggtgtgg cgccgccaga
      481 ccttcgggtc cttctcgccg gcgtcaccga gggcgcggca gattccgccg aagaggatcc
      541 agacctcatc gacggtggcg tccttgcgga tcgctcccgc cttctgcgcc ttgcgaacca
      601 gccgggccat gtggcccttg acccgcgcct tgacctcgat cagctcttcg gagcttcccg
      661 accagtagat gcccgcctga aagctgagat cgctggcccg ggtggcagct ttgcggagcg
      721 cctcaccgaa ggcggcgccg ggatcgtcgc tttccagggc gtcgaggatg tcctcgtcga
      781 agcggttcat ccggcggatc aggaccgcgg caacgaggtc gtccttggtc tcgaagttcc
      841 ggtagacggt gcccttgccc accccggcct tctcggcgat ctccggcacg ccggcctcga
      901 tgccgtgctc ggcgaagacc tgttcggccg cggcgatcac tttctcgcga ttgcggaccg
      961 cgtcggcgcg gcgcggaacc tcgacgcttt cggttacccc ggcagggagc ccggcgtcga
     1021 ccggattcat cgttcgtcgg ccgccagggc gggttcgggg agaacgctgg ccgcgccgat
     1081 ttcctcggtg aagatcgcgt ggcgatggcc ggagcgcggg atcaggaggg cgagcaaccc
     1141 ggcgaagatc gagactccgg ctccgaccag aaacgcggtc cggaacccgt cgctggtcgg
     1201 aaagccggtc gcggcgagga tcgtgccggt caggatcgag gcggtgacct gggagcccag
     1261 cgaggagccg accgagcgga tcagcgtgtt gaagccggtc gcctcaccgg tgcgttccag
     1321 cggcactgcc tccatgatca ggttcggcat cgccgcgtag gcgagtccca ctccgatcga
     1381 ggagaggacg ttccagagca cgatcgaaag cgccgaacca tggtcgagcc ccagcccgat
     1441 cagtccgatg ccgctgatca gcccgccaag cgcgagtggg accctgtttc cgaaccgggc
     1501 tccgagatgc ccggaaagcg gcccggccgg cagcatcatc aaggctgcgg gaagcatcac
     1561 caacccggcc tgggtcgccg aaaagccgaa accgtagccg gtcgaatcgg gtgactcgac
     1621 cagctgcgga atcaggatga atgaagcgaa catgccgaag ccgatcaaca gggtggcggt
     1681 gttggtcatc gcgaccctgg gttcggccag cagggagaca tcggccagcg gctccctggt
     1741 ccgcttctgc agccagaccc agaaggtgat caccacgaat ccggcgagga tcagtccgat
     1801 cgtcttcgtg ctggtccagc cccagttgtt ggcctccgag atcgcataca tcgggaccga
     1861 aagcccgacc gccagcacgg ctgcgccccg gtagtcaacc ttcgccggaa cccggtttgg
     1921 tgactcgggc acgaagatca gcgccgcggc cgcggccatc acggccattg ccgcgccgag
     1981 ccagaagatc cagtggtagg aggcgtggtc aactatcagc ccgccgacga ccaggccgat
     2041 gccgccaccg atgccgagcg tcgccgacat caggccgacc gaggccgaga ccttttcggg
     2101 cgggaactcg tcacggatga ttccgaaaca gagagggaag attccgccgc cgacgccctg
     2161 gaggatgcgg cccgcaacga tggcggggac gctgtctccg agggccgaga ctgtggagcc
     2221 ggcggcgaag attgagaggg cgatgacgag cagccggcgc ttgccgaaca tgtctccaag
     2281 gcgcccgaag atcggggtga agatggcagc cgagatcagg tagccggtga ggacccaggc
     2341 gatcccggtc gcgtcggtgt ggaactcctc catcagttcg gccagggccg gaatcagggt
     2401 ggtctgggcc agcgcgaagg aaagcgcggc caggctgagg atcaggagcg tgcgggtcgg
     2461 atggggtcgg tccgacccgg cggacgattg actaaacgga ctcaccggtc cggatagtaa
     2521 cagccaagcg gacccgcggg tccgctaagc gccgagtact tcggtcacat aggcgttgct
     2581 gaagttccgg ttcgggtcga gctcgtcacg caccttctgg aagtcggccc agcgggggaa
     2641 gctcggggcg aggtcctccg ccgtccggaa gtggagcttg ccccagtgcg gccggccgtc
     2701 gt
//
LOCUS       DAOQKN010000259         2691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_26836_length_2691_cov_3.918058, whole genome shotgun sequence.
ACCESSION   DAOQKN010000259 DAOQKN010000000
VERSION     DAOQKN010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26836_length_2691_cov_3.918058"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1351)
                     /locus_tag="PLJ64_12120"
     CDS             complement(<1..1351)
                     /locus_tag="PLJ64_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HNC94273.1"
                     /translation="MIVLDATVVNVALPAIQDDLGFSQSSLAWVVNAYLIAFGGLLLL
                     SGRLGDLISRRDVFLVGLVVFTTASLLCGFAQSDVMLVAFRFLAGIGGALTAAVVLGM
                     IVTMFPEPSEQAKAIGVYAFVASAGGSVGLLAGGILTQALSWHWIFFINIPIGIATGY
                     FSLKLLERDKGLGFSHGADILGAVLITASLMLGVFTIVEPAAVHGWGDTTTLLCSAGS
                     FLLLIAFLVRESTVANPLVPLRIFKSRNVSGANAVQALTVAGMFGLFFLGVLYLQEVL
                     GYDALQTGIAFLPTTLVMGTLSVRFSEPLIMRFGARNCLIVGLVLIAIGLALFARVPV
                     DGNYWTDVLPSTLLLGSGVGVCFPSLMTLAMSGATRADAGLASGLVNTTAQVGAAIGL
                     AVLATIAATKTDNLLDAGEPRPEALVAGYEVAFWVAAGLVVVALIVAFIVLEPARPQE
                     "
     gene            complement(1549..>2691)
                     /locus_tag="PLJ64_12125"
     CDS             complement(1549..>2691)
                     /locus_tag="PLJ64_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HNC94274.1"
                     /translation="DPGNFATNVAAGAEYGYMLLWTVLSANLMAMLIQFLSAKMGIAT
                     GKNLPQLCREHFPRRVTWGLWVQAEIIAMATDLAEIVGAAIALNLLFGTPLFVGGLIG
                     SAIAFVLLALRNRGYRRFETAIIGLLGIILAGFLYESFKIGPDASGVLNGFVPELQGT
                     DSLLLAVGIIGATVMPHVIYLHSALTQDRIQPRDDRERHSLIDFQKFDVGLAMGIAGF
                     INMSMLAIAAAVLGGTGIDSIEGAFDGLKDLVSPAAGIAFGLALLASGIAASSVGTFA
                     GQVVMQGFINRMIPLFLRRAITMAPALIILAIGLDPTRSLVISQVILSFGIPFALVPL
                     IVLTSRRDVMGEGFENHRLTTIVASIFAAIIIALNLFLLFQTFTGN"
BASE COUNT          530 a          801 c          987 g          373 t
ORIGIN      
        1 cctcctgtgg gcgggccggc tcgagcacga tgaaggcgac gatcagcgcg accacgacca
       61 gcccggcggc gacccagaag gcaacctcgt agccggcgac cagggcctcg ggccggggtt
      121 cgccggcgtc gaggaggttg tcggtcttgg ttgcggcgat ggtcgcgagc acggccagcc
      181 cgatcgcggc accgacctgg gcggtcgtat tgacaagccc ggaggcgaga ccggcgtcgg
      241 ccctggtcgc accggacatg gccagggtca tgagtgaggg gaagcagacc ccgaccccgg
      301 agccgagcag gagggtggag ggcagcacgt cggtccagta gttgccgtca accgggaccc
      361 gggcgaagag ggccaggccg atcgcgatca gcaccagccc gacgatcagg cagtttctgg
      421 cgccgaaacg catgatcagc ggctccgaga acctgaccga aagcgtgccc atgaccaggg
      481 tggtgggaag gaaggcgatg ccggtctgga gcgcgtcgta gccgaggact tcctgcaggt
      541 agagcacccc gaggaagaag agtccgaaca tgccggccac ggtcagggcc tggacggcgt
      601 tggccccgga gacgttgcgg gacttgaaga tccgcagcgg gaccagcgga ttggcgaccg
      661 ttgattcccg cacgaggaag gcgatcagga gcaggaagga accggccgag cagagcaggg
      721 tcgtcgtgtc tccccagccg tgcacggcgg ccggttcgac gatcgtgaag acaccgagca
      781 tcagcgaggc ggtgatcagc acggcgccga ggatgtcggc tccgtgggag aagccgagtc
      841 ccttgtcgcg ctcgagcaac ttcagtgaga agtagccggt agcgatgccg atcgggatgt
      901 tgatgaagaa gatccagtgc caggagagcg cctgggtgag gatgccgccg gccaggaggc
      961 cgaccgaacc gccggccgag gcgacgaagg catagacgcc gatcgccttc gcctgttcgc
     1021 tcggctcggg gaacatggtc acgatcatgc ccagcacgac cgcggcggtc agggcccccc
     1081 cgatcccggc caggaaacgg aaggcgacca gcatcacatc actctgggcg aaaccgcaaa
     1141 gtagggacgc ggtggtgaaa acgaccaggc cgacgaggaa cacgtcgcgc cgggagatca
     1201 ggtcgcccag cctccccgag aggagcagca gcccgccgaa ggcgatcagg tacgcgttga
     1261 ccacccaggc caggctggat tgggaaaacc cgaggtcgtc ctggattgcc ggcagggcga
     1321 cgttcacgac ggttgcgtcg agaacgatca tcagtacccc ggcgcacagg acgtagagcg
     1381 caatccagcg ggaacgatcc ggatccttcg gatggtcggg ctggttatcg gagtctgaat
     1441 cggatggaat taccgtgttt tcagactggt cggtcaggcg gctgagacta acaaaagtgg
     1501 ccgttcggcc cgggccgccg cggacggtcc ggtccggtcc ggttggcctc agtttccggt
     1561 gaaggtctgg aagagcagga agaggttgag cgcgatgatg atcgcggcga agatcgaggc
     1621 gacgatcgtg gtcagccggt ggttttcaaa gccctcgccc attacgtcgc ggcgcgaggt
     1681 gagcacgatc agcggcacca gcgcgaaggg aatgccgaag gagaggatga cctggctgat
     1741 caccagcgag cgggtcgggt cgaggccgat cgcgaggatg atcagggcgg gagccatcgt
     1801 gatcgcccgc cgcaggaaga gcgggatcat ccggttgatg aagccctgca tgaccacctg
     1861 cccggcgaag gtgccgacgc tggaggcggc gatccccgag gcgagcaggg ccaggccgaa
     1921 ggcgataccg gcggcggggc tgaccagatc cttgaggccg tcgaaggcgc cttcgatcga
     1981 gtcgatgccg gtgccgccga gcacggccgc ggcgatcgcc agcatcgaca tgttgatgaa
     2041 gccggcgatg cccatcgcca gccccacgtc aaacttctgg aagtcgatca gggagtggcg
     2101 ctcgcgatca tccctgggct ggatccggtc ctgggtcagg gccgagtgca ggtagatcac
     2161 gtggggcatg accgtggcac cgatgattcc gacggcgagc agcaggctgt cggttccctg
     2221 gagctccggc acgaacccgt tgagcacgcc ggaagcgtcg gggccgatct tgaacgactc
     2281 gtagaggaac cccgcgagga tgatcccgag cagtccgatg atcgccgtct cgaaacggcg
     2341 gtagccgcgg ttgcgcaggg cgagcagcac gaaggcgatc gcgctgccga tcaggccgcc
     2401 gacgaatagg ggggtgccga agagcaggtt gagggcgatc gccgcgccga cgatctcggc
     2461 caggtcggtc gccatggcga tgatctccgc ctggacccac aggccccagg taacccgccg
     2521 ggggaagtgc tcgcggcaga gctggggcag atttttgccg gtggcgatgc ccatcttggc
     2581 cgagagaaac tggatcagca tcgccatcag gttggcgctg agcaccgtcc agagcagcat
     2641 gtagccgtac tcggcgccgg ccgcgacatt ggtcgcgaag ttgcccgggt c
//
LOCUS       DAOQKN010000260         2650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_27604_length_2650_cov_5.242004, whole genome shotgun sequence.
ACCESSION   DAOQKN010000260 DAOQKN010000000
VERSION     DAOQKN010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27604_length_2650_cov_5.242004"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1284)
                     /locus_tag="PLJ64_12130"
     CDS             complement(<1..1284)
                     /locus_tag="PLJ64_12130"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNC94275.1"
                     /translation="MEEKSARRGFLLSKGWLQAVIIVMLLGFTVLGILAYRTYEDGAP
                     VPVKVLDPQGQLLFDGDDITAGQQVFLHNGLMEYGSVFGHGAYLGPDYTADYLRRSSN
                     IAIAENGGPPVDEDGMELADDPDPGPASDIARQKTINQFRENQFDEESNELTFSQTQT
                     DAFNKLIPYYTRFFSADNTENGLRKEAITDPEDLRNLTAFFAWSAWASAAERPGKDYS
                     YTNNWPPEPRVDNSPTANALVWSAISLVALLGGIGLLFGAFGRYRDLGWHGREQTVVS
                     FRDPSTVALTPGQKSTAWFFFVMAALFVIQCFVGAAVQHYRAELTSFFGFDLAAILPY
                     NLLRTWHVQLSLFWVATSFVAAGIFLAPMIARREPRGQGKLGYFLLVALAVVVFGTLI
                     GSYLGIHGVLEDAATNWFGLQGFEYLDLARLWQVLL"
     gene            1438..>2650
                     /locus_tag="PLJ64_12135"
     CDS             1438..>2650
                     /locus_tag="PLJ64_12135"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNC94276.1"
                     /translation="MTATATARRVPPMVDESVLSQIWKVSTAKNEIFDESRARYGDAF
                     GMNINRGKFYFLAHPDQVKEVFTASPEVLHAGKGNEILATALGEHSVLLLDGSEHMRQ
                     RKLLLPSFHGEKLEAQTGAMERISEEVVASIPRGESFHMRETSQKIALEVILEIVLGT
                     ASTGEQHDRLGKATRELLEWIGKGYRLALSSVFGPRSRPIKRMYKPALAPLDQGLYEL
                     IAERRQATGLEDRHDILSMLLLACDEDGQPMTDLEIRDELVTLIVAGHETTATALAWA
                     FERLTRVPGGTERLHEEAVAGDGEYARWVSQEALRLRPVLDFVLRRVMEPIEIGGYQF
                     GEGDVLAPSIYLVHRREDVYPDPLAFKPERWDGVKPGTYTWFPFGGGVRRCIGMQFAM
                     AEMEIVMKAVAR"
BASE COUNT          487 a          832 c          881 g          450 t
ORIGIN      
        1 cagcagcacc tgccagaggc gggcgaggtc gagatactcg aatccctgaa gcccgaacca
       61 gttggtcgcg gcatcctcca gcacgccgtg gatccccaga taggaaccga tcagggtgcc
      121 gaagacgacg accgccagcg cgacgagcag gaagtagccc agtttgccct gccccctcgg
      181 ttcgcggcgc gcgatcatcg gggcgaggaa gatgccggcc gccacgaacg aggtggcgac
      241 ccagaagagc gacaactgga cgtgccaggt ccgaagcagg ttgtagggaa ggatcgcggc
      301 caggtcgaaa ccgaagaaac tggttaattc ggcccggtag tgctgcacgg ccgccccgac
      361 gaaacattgg atcacgaaca gggcggccat cacgaagaag aaccaggccg tcgacttctg
      421 gccgggggtc aacgccacgg tcgacgggtc acggaaggaa accaccgtct gttcgcggcc
      481 gtgccagccg aggtcgcggt agcgtccgaa ggcgccaaac agcaggccga tcccgccgag
      541 cagggcgacc agggagatcg ccgaccagac gagggcgttg gcggtcgggc tgttgtccac
      601 cctcggttcg ggcggccagt tgttggtgta cgagtagtcc ttgcccggtc gttcggccgc
      661 ggaagcccag gctgaccagg cgaagaaggc cgtcaggttg cggagatcct cgggatcggt
      721 gatcgcctcc tttcgcagcc cgttctcggt gttgtcagcc gagaagaagc gcgtgtaata
      781 cgggatcagc ttgttgaagg cgtcggtctg ggtctgcgag aaagtgagtt cgttggactc
      841 ctcgtcgaac tggttctcgc ggaactggtt gatcgtcttc tgccgggcga tgtccgaggc
      901 cggcccgggg tcgggatcgt cggcgagctc catcccgtcc tcgtcgaccg gtggcccgcc
      961 gttctcggcg attgcgatgt tggacgagcg acgcagatag tccgcggtgt agtcgggccc
     1021 gaggtaggcg ccgtggccga agaccgagcc gtactccatg agtccgttgt gcaggaagac
     1081 ctgctggccg gccgtgatgt cgtcaccgtc gaaaagcagt tgcccctggg ggtcaaggac
     1141 cttgaccggt accggtgcgc cgtcttcata ggtgcgatag gccaggatgc cgagcacggt
     1201 gaagccgagc agcatgacga tgatgaccgc ctgcagccag cctttgctga gcaggaaccc
     1261 tcgacgggca gacttctcct ccacggatct ccttccgcag ttcgcccttg cgggcgagta
     1321 tggactggga cgagtgcttc tggcaggctt tgtaacacct caaccggcaa atgtccatgt
     1381 ggacttctac ggaacttgat gtgaaacgaa gcgttgtcaa tcatctagtg tgaagccatg
     1441 actgccaccg ctaccgcgcg ccgcgtcccg cccatggtcg acgagtcagt gctctcccag
     1501 atctggaaag tctcgaccgc caaaaacgaa atcttcgacg agtcacgggc caggtacggc
     1561 gatgccttcg ggatgaacat caaccgcggg aagttctatt tcctggccca ccccgatcag
     1621 gtcaaggagg tcttcaccgc ctccccggaa gtgctccacg ccggcaaggg caacgagatc
     1681 cttgcgacgg cgctgggcga gcattcggta ctgctgctcg acggctccga acacatgcgg
     1741 caacgcaagc tgctcctccc ttccttccac ggcgagaagc tggaagcgca gaccggcgcg
     1801 atggagcgga tctcggagga ggtggtggct tcaatcccca ggggcgagag cttccacatg
     1861 cgggagacca gccagaagat cgcgctcgag gtcatcctcg agatcgtgct cggcaccgcc
     1921 tcgaccggtg agcagcatga ccgcctcggc aaggccacgc gcgagttgct cgaatggatc
     1981 ggcaagggtt accgcctcgc cctttcttcc gtgtttggcc cgcgcagccg gccgatcaaa
     2041 cggatgtaca agccggccct ggcgccgctc gatcagggcc tctacgagct gatcgccgag
     2101 cgtcgccagg cgaccggcct ggaggatcgc catgacatcc tctcgatgct gctgctggcc
     2161 tgcgacgaag acggtcagcc gatgaccgac ctggagatcc gcgacgaact cgtcaccctg
     2221 atcgtggctg gccacgagac cacggcgacc gcgctggcct gggccttcga acggctgacc
     2281 cgggtgcccg gcggcaccga gcggctccac gaggaagccg tcgccggcga tggcgaatat
     2341 gcccgctggg tttcccagga ggcgctgcgg ctccggccgg tcctcgactt cgtcctccgc
     2401 cgggtgatgg aaccgatcga gatcggcggc taccagttcg gggaaggcga cgtactcgct
     2461 ccctcgatct acctcgtcca tcgccgcgag gacgtttatc ccgacccgtt ggccttcaag
     2521 ccggagcgct gggacggggt caagcccggc acctacacct ggttcccctt cgggggcggt
     2581 gttcgccgct gcatcggcat gcagttcgcg atggccgaga tggagatcgt gatgaaggcg
     2641 gttgcccggg
//
LOCUS       DAOQKN010000261         2644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_27696_length_2644_cov_4.914253, whole genome shotgun sequence.
ACCESSION   DAOQKN010000261 DAOQKN010000000
VERSION     DAOQKN010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27696_length_2644_cov_4.914253"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PLJ64_12140"
     CDS             <1..201
                     /locus_tag="PLJ64_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94277.1"
                     /translation="DAGYVFAAYMVFLALLLIYLGILGTKFQRINRDIARLGEEVESG
                     REREPAGPAGSPESGSREPTSV"
     gene            194..1525
                     /gene="hemA"
                     /locus_tag="PLJ64_12145"
     CDS             194..1525
                     /gene="hemA"
                     /locus_tag="PLJ64_12145"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HNC94278.1"
                     /translation="MSEVLALGLSHKTAPLELRERLALPEGRAARLMAELTDSGDASE
                     ATVISTCNRTEIYVFAHDPVVAESSILSALASQGGISPTELAGHLYSLRGDDAALHLF
                     RVTAGLDSMIIGESEVQGQVKRAHELALVEGVSGPILNRLFRGALAAGGRARNETGIS
                     RTGVSMASVAVEQATRAIGEVGDRRALIIGAGETSELVAGALRARGAESVFVANRHLE
                     KACDLARKFGGTAAPLTELSEQVAEADLVVSATMSPHHLIDRELLEPVMKERQERPVF
                     LVDLAVPRDIDPRVRELPGVSLTDVDELQVAVARNLGRREAEARRAEHVVQAELELFQ
                     RWLQSLEVMPVIVGLRDKADEIVARVLSENEGRWEAMSEEDRRRVETLARTIVGRMLH
                     EPTLRLKEIAGSDESFEVLATVRGLFGLDSGTDPGDGEVATVTPIRREGNS"
     gene            1573..2391
                     /gene="hemC"
                     /locus_tag="PLJ64_12150"
     CDS             1573..2391
                     /gene="hemC"
                     /locus_tag="PLJ64_12150"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HNC94279.1"
                     /translation="MAEALGPQAELVEVDADLPPEAGDKERFTRGVERLLLDGQAEAA
                     VHSAKDLPGEMTAGLAIGAVPARVDPRDAWIGPGGSIEEIPDGARVGTASLRRRAQLK
                     ALRPDLQMVELRGNVDTRLRKLEAGDADGLVLAMAGLQRLGRDHVVSFAFDEEQMTPA
                     AGQGCLVVQTRTGGEAQVEGISDPNSLTRLTAERAAVVGLGADCSSPVGVHARLEGNW
                     LVIDAFAGRSDGSVWVRDRVEGDPARPESLGRELAERMLRAGASEAMAGSTLSD"
     gene            2391..>2644
                     /locus_tag="PLJ64_12155"
     CDS             2391..>2644
                     /locus_tag="PLJ64_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNC94280.1"
                     /translation="MAEVQQGKVSLVGAGPGDPGLMTARALELISEADVIFYDRLIPP
                     DALAGAREDAELIYVGKMPGTVHNPQEDINRRIVEAGREGR"
BASE COUNT          415 a          901 c          942 g          386 t
ORIGIN      
        1 gacgccggat acgtcttcgc cgcctacatg gtcttcctgg ccctgctctt gatctacctc
       61 ggcatcctcg gcaccaagtt ccagcggatc aaccgtgaca tcgcccggct cggcgaagag
      121 gtcgaatcgg gccgcgagcg ggaaccggcc ggtccggcgg ggtccccgga aagcggcagc
      181 agagagccga ccagtgtctg aggttctcgc actcgggctt tcacacaaga cggctccgct
      241 ggagctgcgc gaaaggctcg ccctgcccga aggccgggcg gcccgcctga tggccgagct
      301 gaccgattcc ggggatgcct cggaagcgac ggtcatctcg acctgcaacc ggaccgagat
      361 ctacgtcttc gcccacgacc cggtggtcgc cgagtccagc atcctttcgg cccttgccag
      421 ccagggcgga atcagcccga ccgaacttgc cggccacctc tactcgctgc gcggcgacga
      481 cgcggcgctc cacctcttcc gggtaaccgc cgggctcgac tcgatgatca tcggcgagtc
      541 cgaggtccag ggccaggtca agcgggctca tgaactggca ctggtcgaag gcgtctccgg
      601 cccgatcctc aaccggctct tccgcggtgc cctcgccgcc gggggccggg ccaggaacga
      661 gaccggaatc agccggaccg gagtctcgat ggcctcggtc gcggttgaac aggcgacccg
      721 ggcgatcggg gaggtcggtg atcgccgggc cctgatcatc ggcgccggcg aaacctcgga
      781 acttgtggcc ggtgccctgc gtgcccgcgg ggccgaaagc gtcttcgtcg ccaaccggca
      841 ccttgaaaag gcctgcgacc tggcccggaa gttcggtggt acggccgcac cgctgaccga
      901 gttgagcgag caggtggccg aggccgacct ggtcgtttcg gcgaccatgt caccccacca
      961 cctgatcgat cgcgaactgc tcgaaccggt catgaaggaa cgccaggagc gtccggtctt
     1021 cctcgtcgac ctggcggtcc cgcgggacat tgacccgcgg gtgcgggaac tccccggggt
     1081 gagcctgacc gacgttgacg aactgcaggt cgcggtggcc cgcaacctcg gccggcgcga
     1141 agctgaagcc cgccgggccg agcatgtcgt ccaggccgag cttgagctct tccagcgctg
     1201 gctccagtcg cttgaggtga tgccggtgat cgtcggcctg cgtgacaagg ccgacgagat
     1261 cgttgcccgt gttctcagcg agaacgaagg ccgctgggag gcgatgagcg aagaggaccg
     1321 gcgccgggtc gagaccctgg cccggacgat cgtcggtcgc atgcttcatg agccgaccct
     1381 gcgcctgaag gagatcgccg gcagcgacga gtccttcgag gtgctcgcca cggtccgtgg
     1441 cctcttcggc ctcgacagcg ggaccgaccc cggcgacggc gaagtcgcca cggtcacccc
     1501 gatccgccgg gaaggcaact cctgagggtc gcaacccgtt ccagtgccct cgccctggcc
     1561 caggccggcc aggtggccga ggcgcttgga ccacaggcgg aactggtcga ggtcgacgcc
     1621 gacctgccac ccgaggccgg ggacaaggag cgcttcaccc gcggggtcga aaggctgctg
     1681 ctcgacggtc aggccgaggc cgcggtccat tcggccaagg acctgcccgg cgagatgacc
     1741 gcggggctcg cgatcggcgc agtgccggcc cgggttgatc cccgggacgc ctggatcggc
     1801 cccggcggtt cgatcgagga aatccccgac ggcgcccgtg tcggcacggc cagtctgcgc
     1861 cggcgagccc agctcaaggc gctccggccg gatctgcaga tggtcgagct ccgcggcaac
     1921 gttgacaccc ggttgcgcaa gcttgaggcc ggggatgccg acggcctggt gctggcgatg
     1981 gccggccttc agcgactcgg ccgtgaccac gtggtcagct tcgccttcga tgaagagcag
     2041 atgacgccgg ccgccgggca gggctgcctg gtcgtccaga cccgcaccgg tggcgaggcg
     2101 caggtcgaag ggatctccga ccccaactcc ctgacccggc tgacggccga gcgggcggcg
     2161 gtggtcggtc tgggcgccga ctgttcgagc ccggtcggtg ttcacgcccg gcttgagggc
     2221 aactggctgg tgatcgacgc cttcgccggc cgctccgacg gcagcgtctg ggtgcgcgac
     2281 cgggtcgaag gggacccggc ccggcccgag tcgcttggcc gggaactcgc ggagcggatg
     2341 ctgagggccg gggcgagcga agcgatggcc gggtctaccc tgtctgactg atggccgaag
     2401 tccaacaagg caaagtcagc ctggtcggag cgggcccggg tgatccgggg ctgatgacgg
     2461 cccgtgcgct cgaactgatc agcgaggccg atgtgatctt ctacgaccgg ctgattccgc
     2521 cggatgcgct ggccggagcc cgcgaggatg ccgagctgat ctacgtcggc aagatgcccg
     2581 gcaccgtcca caacccgcag gaagacatca accggcggat cgtcgaggcg gggcgggaag
     2641 gccg
//
LOCUS       DAOQKN010000262         2633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_27915_length_2633_cov_6.062452, whole genome shotgun sequence.
ACCESSION   DAOQKN010000262 DAOQKN010000000
VERSION     DAOQKN010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27915_length_2633_cov_6.062452"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="PLJ64_12160"
     CDS             complement(<1..825)
                     /locus_tag="PLJ64_12160"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HNC94281.1"
                     /translation="MLRVSGHSERTDVGRQRQANEDSYMARAPLFVVADGMGGAQAGE
                     VASLTAIQAFQAGLPEGSPEAAIEQTIGVANRNIYEQAHADPSLSGMGTTITVAAVDS
                     DSEQVIIGHVGDSRAYRLRDGIIQRLTKDHSLVEEMRRRGQITEEQAEDHPQRSIITR
                     ALGPEPEVQADLTAVPSEPGDIFLLCSDGLTTMIGDEKIRDILMGATSLDAAARTLVD
                     EANRAGGRDNITVIVFQVEDPARPLPKGEQPTMIRRGGAARGASRRAAGRRRGDGET"
     gene            complement(845..1309)
                     /locus_tag="PLJ64_12165"
     CDS             complement(845..1309)
                     /locus_tag="PLJ64_12165"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNC94282.1"
                     /translation="MDYEPISVALKFGFLAVLFLFLLWIATSARKDLKRNSGGNFESD
                     TTIGAMPGGSYDAWLVVVRGGGLEAGTRFDVFGGVTLGRSSDADIAFTDRYASGLHAR
                     LYPRGDRYFLEDMNSTNGTLLDGGAVTSDAEVRDGSMIEIGDTAFRFELDRS"
     gene            complement(1328..2095)
                     /locus_tag="PLJ64_12170"
     CDS             complement(1328..2095)
                     /locus_tag="PLJ64_12170"
                     /inference="COORDINATES: protein motif:HMM:NF023818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HNC94283.1"
                     /translation="MSVFRNLEARIEGLVEGVFSRAFSSEVQPVELARKLAKEMDSHK
                     TASVSRVYVPNEYTVHLSEKDRDKLEGYERSLEQELSGYLLEHARRKGYDLLTRPEVH
                     FNTDSRLRLGEFGIQARLVKPPVREGEMPRQADEGHTMVYKVAPQPAADPTARAPERP
                     SLTRAVVDLDDRRYVLDGPRAVLGRSDDAECVLRDPNVSRRHAELRQDAAGQWEIFDL
                     GSTNGVKINGRRVTEAPLREGDQVTIGTTTFRFGIER"
     gene            complement(2123..>2633)
                     /locus_tag="PLJ64_12175"
     CDS             complement(2123..>2633)
                     /locus_tag="PLJ64_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94284.1"
                     /translation="LAAWLSVIAAMPGAALVVLLVSVPPALLIRGSGRPLALAVVGPL
                     LGTLGMAPFLPLLAAVATDWRDRVVVAVTGLVFTAFAESITGRALLFGRIEKAGTGWE
                     DSLSSAVTGLIVPVVSSPSFLLSVAVWAAVALLVGAIVGWTRGRSGGPVHEPLTIAPV
                     GSSRVPNAN"
BASE COUNT          422 a          865 c          914 g          432 t
ORIGIN      
        1 ggtttcaccg tcgccccggc ggcggccggc ggcgcggcgg gaagctccgc gggctgcgcc
       61 gccgcggcgg atcatggtcg gctgctcgcc cttaggcagc ggccgggccg gatcctcgac
      121 ctggaagacg atcacggtga tgttgtcccg gccaccggcc cggttggcct cgtcgaccag
      181 ggtgcgtgcg gccgcgtcga ggctggtcgc ccccatcagg atgtcgcgga tcttctcgtc
      241 cccgatcatc gtggtcagtc cgtcggagca gagcaggaag atgtcgcccg gctcggaggg
      301 caccgcggtg aggtcggcct ggacctccgg ctccgggcca agcgcccggg tgatgatcga
      361 acgctgggga tggtcctcgg cctgctcctc ggtgatctgg ccgcggcgtc gcatctcctc
      421 gaccagggag tggtccttgg tcaggcgctg gatgattccg tcgcggaggc ggtaggcgcg
      481 ggagtcgccg acgtggccga tgattacctg ctcggaatcg gagtcgaccg cggccaccgt
      541 gatcgtcgtg cccatccccg aaagcgacgg gtcggcgtga gcctgctcgt agatgttccg
      601 gttcgcgact ccgatcgtct gttcgatcgc cgcttcggga ctgccctccg gcaggccggc
      661 ctggaaggcc tggatcgcgg tcagcgaggc aacctcgccg gcctgggcac cgcccatgcc
      721 gtcggcgacc acgaaaagcg gggcgcgggc catgtaggaa tcctcattgg cctggcgctg
      781 gcggccgacg tcggtccgct cggaatgtcc cgagactctc agcatttgga tgtttccgag
      841 ctactcatga gcggtcaagt tcaaacctga aagcggtgtc gccgatctcg atcatgctcc
      901 cgtcccggac ctcggcgtcg gatgtcaccg cgccgccgtc cagcagagtg ccgttggtcg
      961 agttcatgtc ttcaaggaag tatcggtcac cgcgagggta gagcctcgcg tgaaggccgg
     1021 acgcgtaacg atcggtgaag gcgatgtccg cgtcggaaga gcggcccagg gtcaccccgc
     1081 cgaagacgtc aaagcgggtc cccgcttcga gtccgcctcc gcggaccacg accagccagg
     1141 cgtcgtagga gccgccgggc atcgctccga tcgtggtgtc ggactcgaaa ttgccgcccg
     1201 agttccgctt caggtctttc ctcgcgctgg tcgcgatcca gagcaggaac aggaagagga
     1261 cggccagaaa tccgaacttg agggcaactg aaatcggttc gtagtccaat tgcgcggccg
     1321 gcgctttcta ccgttcgatt ccgaagcgga aggtcgtggt tccgatcgtg acctggtcgc
     1381 cttcgcgaag cggtgcttcc gtcacccttc gcccgttgat cttgaccccg ttggtcgagc
     1441 cgaggtcgaa gatctcccat tgaccggccg catcctgccg gagctcggcg tggcggcggg
     1501 aaacgttcgg gtcccggagt acgcactcgg cgtcgtcgga gcggccgagg acggcgcgcg
     1561 gtccgtcgag gacgtagcgc cggtcatcga ggtcgaccac ggcgcgggtc agcgagggcc
     1621 tttcgggggc gcgtgcggtc gggtcggccg ccggctgcgg cgccaccttg tagaccatgg
     1681 tgtggccctc gtcggcctgg cggggcatct cgccctcgcg gaccggaggc ttgaccaggc
     1741 gggcctggat tccgaactcg ccgagtcgca gccggctgtc ggtgttgaaa tgaacctcgg
     1801 gcctggtcag caggtcgtaa cccttgcggc gcgcatgctc cagcaggtac ccggagagtt
     1861 cctgttccag agatcgctcg taaccctcga gcttgtcgcg gtccttttcc gagaggtgca
     1921 ccgtgtactc gttcggcaca tagacccggg agaccgacgc ggtcttgtgg gagtccatct
     1981 ccttggccag cttgcgggca agttcaaccg gctggacctc tgaagaaaag gcacggctga
     2041 acaccccttc gacgaggccc tcgatgcggg cttcgaggtt gcggaaaacg ctcatcgccg
     2101 cgaatccgtg aagccaatcg gctcagttgg cattcgggac acgacttgat cctacgggag
     2161 cgatggtcag tggctcgtgg accgggccgc cgcttcgccc tctggtccag cccacgatgg
     2221 ccccgacaag cagcgccacc gccgcccaga cggccaccga aaggaggaag gaaggcgagc
     2281 tcacgacagg gacgatcagc ccggttaccg ctgacgaaag cgaatcctcc caacccgtcc
     2341 cggccttctc gatcctgccg aagagcaggg cgcgcccggt gatcgactcg gcaaaggcgg
     2401 tgaagacgag accggtcaca gcgaccacca cccggtcccg ccagtcggtc gcgaccgcgg
     2461 ccagcaaggg cagaaacggc gccatgccca gggttccgag caggggtccg accacggcga
     2521 gggccagcgg gcgcccggac ccgcggatca ggagggccgg tgggaccgat acgagcagga
     2581 cgaccaaagc cgctcccggc atcgcggcga tcacgctgag ccaggcggcg agc
//
LOCUS       DAOQKN010000263         2627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_28028_length_2627_cov_5.428072, whole genome shotgun sequence.
ACCESSION   DAOQKN010000263 DAOQKN010000000
VERSION     DAOQKN010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28028_length_2627_cov_5.428072"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="PLJ64_12180"
     CDS             <1..220
                     /locus_tag="PLJ64_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94285.1"
                     /translation="DDPTGDDSDNPSSDDPVEDLTDDEAAQSGGPKSNAGMAKAIRAQ
                     KRRVAAKKCQKIGKRSKRKACLKRARSI"
     gene            332..2032
                     /locus_tag="PLJ64_12185"
     CDS             332..2032
                     /locus_tag="PLJ64_12185"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNC94286.1"
                     /translation="MDQSVWNRHRFALVAAIVLSAAGLMVSLLPQTAGAVLAGENGRI
                     VMVSGRPPETDATARLYLLPVPSNSVGGGLISNPIVPAGGQYRHPSWSPDRTKVVFAN
                     GSGGIYDLFVIDLEAGTGPVQITPTEGAGANNLSEDRPAWSPDGTKIAYEHQPSPGST
                     DRQIRIQPADDLQLPITTTDLTTAGAPFEGKPAWTPDSQTIYFHRNDPNTAANSDIYR
                     KPAAGGAETLAIPDSGISEAQPAISPEGDRICFTLTSGGFNATADVLVAPLANPANAI
                     VVSKFANPAVGDYNCTWSPDGQMVAYVNGTFSTGRLVMVKADNTSPLEIELAEAAGFD
                     GNPDWAPDGRPDCPDSSVVTAFDQPATFSTECLDTGPEYEQTEVREYAGTQPAHGTLE
                     QELAGDPFTYTPNQGFSGTDQFEVRSFDELGFGVDTGLVTITVKPQVRCGGKPATVKG
                     TPGPDILTGTLGRDVFAGFGGNDLILGLGGRDLACGGPGKDRLNGAGGKDRLIGGPGN
                     DLLNGGAAADLLSGGAGRDRLLGGAGRDRLRAGPGRDVCKGGPGRDRAPRCERRAGIP
                     "
     gene            2072..2488
                     /locus_tag="PLJ64_12190"
     CDS             2072..2488
                     /locus_tag="PLJ64_12190"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNC94287.1"
                     /translation="MTVRLNPYLNFREEAREAMTFYQSVLGGELTINSFADFHASEDP
                     AEQEKVMHAHLVTADGFNLMGADTPNSMEFNGQSGVSVSLSGDDEETLRRWFEGLSEG
                     GTVVMPFEQAPWGATFGMVVDRFGTSWLVNAGPESQ"
BASE COUNT          462 a          903 c          883 g          379 t
ORIGIN      
        1 ggacgacccg accggggatg actccgacaa cccgtcttcg gatgatccgg tcgaggatct
       61 gaccgatgat gaggctgccc agtccggcgg gccgaagtcg aatgccggca tggcgaaggc
      121 gatcagggcc cagaagcgcc gggttgccgc caagaagtgc cagaagatcg gcaagaggtc
      181 gaagcgcaag gcctgcctga agcgcgcccg ctcgatctga agttcccctc gacgacagga
      241 tcagccaccc gggcttgacg gcgtgacggg cctcggttag gttcggtgtg tatccgatgc
      301 cggcaggtct gggccggcaa gcgaggcgga gatggatcaa tcagtctgga accggcaccg
      361 gtttgccctg gtcgccgcga tcgtcctttc ggcagcgggg ctgatggtca gcctgctgcc
      421 tcaaacagcc ggggcggtgc tggccggcga gaacggccgg atcgtcatgg tcagcggcag
      481 accgcccgag accgacgcca ccgcgcgtct gtatctcctg cccgtgccga gtaactcggt
      541 cgggggaggg ttgatcagca atccaatcgt gcctgccggc gggcagtacc ggcatccttc
      601 gtggtcgccg gaccggacca aggtcgtgtt cgccaacggc agcggtggca tctacgacct
      661 gttcgtgatc gatctcgaag cgggcaccgg gccggtccag atcaccccga ccgagggagc
      721 cggggccaac aacctgagcg aagaccgtcc ggcctggtcg ccggacggca cgaagatcgc
      781 ctacgagcac cagccctccc cgggaagcac cgaccggcaa atccggatcc agcccgcgga
      841 tgacctccag ttgccgatca cgacgaccga cctgaccacc gccggggcgc cgttcgaagg
      901 caagcccgcc tggacccccg actcccagac catctacttt catcgcaacg accccaacac
      961 cgccgccaac tcggacatct accgcaagcc ggcagccggg ggcgccgaga cgctggcgat
     1021 cccggactcg gggataagcg aagcccagcc ggcaatctcg cccgagggcg accgcatctg
     1081 cttcaccctg accagcggcg gcttcaacgc gaccgccgac gtcctggtgg cgccgctcgc
     1141 gaacccggcc aacgcgatcg tcgtctccaa gttcgcgaat cccgcggtcg gtgactacaa
     1201 ctgcacctgg tcgcctgacg gtcagatggt tgcctacgtc aacggcacct tcagcaccgg
     1261 tcgcctggtc atggtcaagg ccgacaacac cagcccgctc gagatcgagt tggccgaagc
     1321 cgccggcttc gacggcaacc ccgactgggc cccggacggc cggccggatt gcccggacag
     1381 ctcggtcgtc accgcgttcg accagccggc caccttcagc accgaatgcc tcgacaccgg
     1441 cccggagtac gagcagaccg aagtccgcga gtacgccggc acccagccgg cccacgggac
     1501 gcttgaacag gaactcgccg gcgacccgtt cacctatacc ccgaaccagg gtttcagcgg
     1561 gaccgaccag ttcgaggttc ggagcttcga cgaactgggg ttcggggtcg acacgggcct
     1621 ggtgaccatc accgtcaagc cgcaggttcg ctgtggcggc aaaccggcca cggtgaaggg
     1681 aacgcccggc ccggacatcc tgaccggcac cctgggccgt gacgtcttcg ccggctttgg
     1741 cgggaatgac ctgatcctcg ggctcggagg cagggacctt gcctgcgggg ggccgggcaa
     1801 ggaccgtctg aacggcgccg gtggcaagga tcgccttatc ggtggaccgg gcaatgatct
     1861 gctgaacggt ggcgccgccg ccgacctgct gagcggtgga gcgggccgcg accgactgct
     1921 cggcggggcg ggaagggacc ggctccgcgc cggccccggc cgtgacgtct gcaagggcgg
     1981 acccgggcgc gaccgggctc cccgttgcga gcgccgggcc ggaatcccct gagtagtatc
     2041 ggtccggatc ccaaccgagg agaggaccgg catgaccgtt cgactgaatc cgtacctgaa
     2101 cttccgcgag gaagcccgtg aggcgatgac cttctaccag tcggtgctgg gcggcgagct
     2161 cacgatcaac tcgtttgccg acttccacgc cagcgaggat ccggccgagc aggaaaaggt
     2221 gatgcacgcg cacctggtca ccgcagacgg gttcaacctg atgggcgccg acacccccaa
     2281 ctcgatggaa ttcaacgggc agtcgggagt gtccgtttcg ctcagcggtg acgacgaaga
     2341 gacgctccgc cgatggttcg aggggctcag cgaaggcgga acggtcgtga tgccgttcga
     2401 gcaggcaccc tggggcgcga ccttcggcat ggtggtcgac cgcttcggca ccagctggct
     2461 ggtcaacgcc ggtcccgagt cgcagtagcg ctcgccggct agagggtggt gccgacttcg
     2521 gattcgagtt cggcctcggt catgccgtcc gggccttccg ccccggcctc ctgcgggcgg
     2581 gccggttcga gcacctcgcc atcgccaacc ccaagctggc gccctac
//
LOCUS       DAOQKN010000264         2601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_28534_length_2601_cov_4.883346, whole genome shotgun sequence.
ACCESSION   DAOQKN010000264 DAOQKN010000000
VERSION     DAOQKN010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28534_length_2601_cov_4.883346"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="PLJ64_12195"
     CDS             <1..434
                     /locus_tag="PLJ64_12195"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNC94288.1"
                     /translation="HHPSSSDDSSASTIGAAITYIAYMLMWFGGDNNSPLGLIGSLAM
                     VLLAPLAASIIQLAVSRQREYAADAGGAAICGNPESLASALLRLEQGAENIPMKGHVS
                     EASEPLYIVKPFKAKGLAGLFSTHPPIEERVKRLRQMRPSL"
     gene            544..891
                     /locus_tag="PLJ64_12200"
     CDS             544..891
                     /locus_tag="PLJ64_12200"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HNC94289.1"
                     /translation="MAEIRVKRIYEDSSPEDGRRVLVDRLWPRGVKKDRIDEWAKELA
                     PSNELRREFHDSDFDFDEFRARYMVELEGHEDEMRQLLGSTDGPLTLLFGSRNLEQNN
                     ATVLAEALGDLTV"
     gene            1025..2197
                     /gene="moeB"
                     /locus_tag="PLJ64_12205"
     CDS             1025..2197
                     /gene="moeB"
                     /locus_tag="PLJ64_12205"
                     /inference="COORDINATES: protein motif:HMM:NF006444.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HNC94290.1"
                     /translation="MAPEATEQQTIEEITPQEAFAELDGGGVKLIDTREPYEYEESHI
                     EGGELIPPGVIADRIEAAVPDKDQRIILQCRSGARSLVAAEALAGLGYTNLANMSGGI
                     LEWEAQGLPVESNVALTPEQRDRYSRHILLPEVGVEGQIKLLEAKVLLLGAGGLGAPT
                     ALYLAAAGIGTIGIVDDDVVDASNLQRQVIHNTSRVGQPKSASARQTIEELNPDVEVI
                     EYNFRLDAGNILEVIEDYDIIVDGADNFPTRYLLNDASVRLKKPVVSASILSFDGQIS
                     TFIPYEGPCYRCLYPTPPPPELAPSCSANGVLGAMAGQMGLLQANEVLKLVLGIGEPL
                     VGRLLLYEALGTRFTELKVRRDPECPICGPNAPKVPESEMGQFPDYEAFCGGHVGS"
     gene            2241..2516
                     /locus_tag="PLJ64_12210"
     CDS             2241..2516
                     /locus_tag="PLJ64_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD family protein"
                     /protein_id="HNC94291.1"
                     /translation="MPSVKIPPVLRKHTGDQTSVDVAGSNVGEVLNALVAEYPETKDQ
                     LFSGEGELNRFVNVYLNDEDVRVLDGLETGVSDTDTVMILPAMAGGE"
BASE COUNT          484 a          872 c          825 g          420 t
ORIGIN      
        1 cgcaccaccc gagctcgagc gacgactcga gtgcctcgac gatcggcgcc gcgatcacct
       61 acatcgccta catgctgatg tggttcggcg gcgacaacaa ttcaccgctc ggcctgatcg
      121 gctctctggc gatggtgctg ctggcgccgc tcgccgcttc gatcatccag ctggccgttt
      181 cgcgccagcg cgaatacgcg gccgacgccg gcggcgccgc gatctgcggc aacccggagt
      241 ccctggcctc ggcgctgctc aggcttgaac agggtgccga gaacatcccg atgaagggtc
      301 acgtgagcga ggcctccgag ccgctctaca tcgtcaagcc cttcaaggcg aagggtctgg
      361 ccggtctttt ctcgacccat cccccgatcg aggaacgggt caagcggctc cgccagatgc
      421 ggccctcgct gtaagggttt cgcaagcagg attttcctcc cgaagcgcag ctggcagtgg
      481 ggcccccgga tcaaatggtc cgggggcctt cccttcgggg cgacgccggg atagcttggg
      541 tccatggccg agatcagggt caagcggatc tacgaggatt caagcccgga agacggccgg
      601 cgggtgctgg tcgaccggct ctggccgcgg ggagtgaaga aggaccggat cgacgagtgg
      661 gcgaaggagc tggccccgag caatgagttg cgccgggagt ttcacgacag cgatttcgac
      721 ttcgatgagt tccgcgcccg ctacatggtc gaactcgagg gccacgagga cgaaatgcgg
      781 caattgctcg gctcgaccga cggaccgttg acgctgctgt tcggttccag gaacctcgaa
      841 cagaacaacg cgacggtatt ggcagaagcc ctcggcgacc tgaccgtctg accggcccgg
      901 tcgggcgctt ttcccgatga cctgtaccct tggtggtcgc ggctgagggg ctggaaaagc
      961 attccggacc tcgctatact ttttgaagta accaaattca gatcgagatt gagggttttt
     1021 gccgatggca ccggaagcga ccgaacagca gacaatcgaa gagatcacgc cccaggaggc
     1081 tttcgccgag ctcgatggtg gcggcgtcaa gctgatcgac acccgcgagc cctacgagta
     1141 cgaggagtct cacatcgagg gcggcgagtt gatcccgccc ggagtgatcg ccgaccggat
     1201 cgaagcggcc gtgcccgaca aggaccagcg gatcatcctg cagtgccgct ccggtgcccg
     1261 ctccctggtc gcggcagaag ctctggcggg gctcggctac acgaacctcg ccaacatgag
     1321 cggcggcatc ctcgaatggg aggcccaggg cctgccggtc gagtcgaacg tcgccctgac
     1381 ccccgaacag cgtgaccgct actcgcggca catcctgctg cccgaggtcg gggtcgaagg
     1441 ccagatcaag ctgctcgaag cgaaggtgct gctgctcggc gccggcggtc tcggagcgcc
     1501 cacagcgctc tatctcgcgg ccgcaggcat tggcacgatc ggcatcgtcg atgacgacgt
     1561 ggtcgacgcc tccaacctgc agcgccaggt gatccacaac accagccggg tcggccagcc
     1621 caagtccgcc tcggcccgcc agacgatcga ggaactgaat cccgacgtcg aggtgatcga
     1681 atacaacttc cggctcgacg ccggcaacat cctcgaggtg atcgaggact acgacatcat
     1741 cgtcgacggg gccgacaact tcccgacccg ctacctgctg aacgacgcct cggtgcggct
     1801 gaagaagccg gtcgtctccg cctcgatcct ttccttcgac gggcagatct cgaccttcat
     1861 cccttacgaa ggcccctgct accgctgcct ctacccgacc ccgccgccgc cggagctcgc
     1921 cccgagctgc tcggccaacg gcgtgctcgg tgcgatggcc gggcagatgg gcctgctcca
     1981 ggcgaacgaa gtcctgaagc tggtgctcgg catcggcgag ccgctggtcg gccgcctgct
     2041 gctctacgaa gcactcggaa cccgcttcac cgaactcaag gtccggcgcg atccggaatg
     2101 cccgatctgc ggtccgaacg cgcccaaggt cccggaatcc gagatgggcc agttccccga
     2161 ctacgaggca ttctgcggag gtcatgtcgg ctcctgagcc ggcgtgaccg acccccgcag
     2221 acgaccgata ggatcagccc atgccgtccg tgaagattcc ccccgttctc cgtaagcaca
     2281 ccggcgatca gacgtcagtc gacgttgccg gttcgaacgt gggcgaggtg ctgaacgcgc
     2341 tcgtcgccga gtaccccgaa acgaaggacc agctcttctc gggcgaaggt gagctgaaca
     2401 ggttcgtcaa cgtctacctg aacgacgaag acgtccgggt gcttgacggc ctcgaaaccg
     2461 gggtcagcga caccgacacc gtgatgatcc tcccggccat ggccggcggc gaataactgc
     2521 aagtcgggcg gggtccgacc cgccctccca gtaaaacccg ccccacccac tcgcacacaa
     2581 gaatggatgg gcggggctcc g
//
LOCUS       DAOQKN010000265         2571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_29140_length_2571_cov_4.840620, whole genome shotgun sequence.
ACCESSION   DAOQKN010000265 DAOQKN010000000
VERSION     DAOQKN010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29140_length_2571_cov_4.840620"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="PLJ64_12215"
     CDS             <1..627
                     /locus_tag="PLJ64_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HNC94292.1"
                     /translation="WDSVLPNGQGPRGHGATAYSSLVLRSLKPARYLEKNLGHAGLGS
                     EAYCHFTSPIRRFPDLLVHRALLSAVGGGEEAPSTGDAAEAALHASEREREAMILERD
                     ADDICAAFLLERELFQEGYEKRFEGEVSGVIRSGAFVRFNGRLGDVYEGFVPARRIRG
                     ERFELNEEETSLVGSKTGRKVSLGDAIAVQVEKVDAPRGRVDLDPADG"
     gene            644..1129
                     /gene="smpB"
                     /locus_tag="PLJ64_12220"
     CDS             644..1129
                     /gene="smpB"
                     /locus_tag="PLJ64_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNC94293.1"
                     /translation="MAKKDKKRAPASGDVATNRRARQKFEVVEKLEAGIALLGTEVKA
                     LREGNAQMGDAYAVVEDGEVWLRNLHIPHYAPATINNHDPERPRKLLLHRAEIERLIG
                     TTQQKGLTLIPTRIYFKGPRAKVELGLARSKEGKDRRREIADRDVRRDVEREFKGRYR
                     S"
     gene            1137..>2571
                     /locus_tag="PLJ64_12225"
     CDS             1137..>2571
                     /locus_tag="PLJ64_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94294.1"
                     /translation="MKPRKLILLALLVAVVLGPGTAVAADEPTTTTAVPTTSSDASTD
                     TAPLTLDQDQINALTEQANGADNDKSVFEKASDFVTDNAPWFIIGIIVLAAIIAGILI
                     MRGRPRKGATQGTPKASKSNKAMRSAGGLSPAEVASSPAQTPSPSELRRRKRAAIQRS
                     REEERARRKAGIENRKAARRGEAPAPVSPATASAAGVTAVTAAGVAAAGGMDPVEAEK
                     QGARDQEVAAGAMARYGAVQAPAAPAETVPAPAFTSGQAIPAPVSPSQTAPAAGVVTP
                     DSVPPAGPGTYESPVPEPVTEPVSEYAAEPAGGYAPEVAETAPAAFAADDTVYEPAAA
                     TPEAEAETSIIHEPSLENPGADAAVGNAAEAFLAGGAAGAAGGLAAARASRPEEPAAE
                     PVEPLEQVIAPEPGMAPEHALADEAPVSPVAGQQAPDAATAAAEERLRAKVAEIKATQ
                     EPPAPPPPVEPPAAGIAPEPVPPREGLP"
BASE COUNT          466 a          858 c          883 g          364 t
ORIGIN      
        1 tgggatagtg ttttgcctaa tggccaaggg ccacggggcc acggggccac ggcctacagc
       61 tcgctggttc tccggtcgct gaagccggcc cgctacctgg agaagaacct cggccacgcc
      121 gggctgggca gcgaggccta ctgccacttc acctcgccga ttcgccgttt tcccgacctc
      181 ctggtccacc gggcgctgct ttcggcggtc gggggcggag aggaggctcc ctcgaccggc
      241 gacgccgcgg aagctgcgct ccacgcttcg gagcgtgaaa gggaagcgat gatcctcgaa
      301 agggatgccg atgacatctg cgccgctttc ctgcttgaac gggagctgtt tcaggaggga
      361 tacgaaaagc gtttcgaggg ggaggtctcc ggggtgatcc gttccggcgc cttcgtccgg
      421 ttcaacgggc ggctcggcga tgtctacgag ggattcgttc ccgcccggcg gatccggggt
      481 gagcggttcg aactgaacga agaagaaact tcgctggtcg gcagcaagac cgggcgcaag
      541 gtcagcctcg gcgatgcgat cgcggtccag gtcgaaaagg tggatgcacc gcgggggagg
      601 gtggatctcg acccggcgga tggctgaaag ctagattccc gtcatggcga agaaggacaa
      661 aaaacgggcg ccggcttccg gtgacgtcgc gaccaaccgg agggcgcggc agaagttcga
      721 ggtggtcgag aagctggagg ccggaattgc gctgctcgga accgaggtca aggcgcttcg
      781 tgaagggaat gcgcagatgg gtgacgccta tgccgtggtc gaggatggcg aagtctggct
      841 gcgcaacctc cacattcccc attacgcgcc ggccaccatc aacaatcacg atcccgagcg
      901 cccgcggaaa cttctgttgc atcgggcgga gatcgaaagg ctgatcggga ccacccagca
      961 gaagggcctg actttgatcc cgacccgcat ctatttcaag ggtccaaggg caaaagtcga
     1021 actcgggctt gcccgcagca aggaaggaaa ggaccggcgg cgtgaaatcg ccgatcgcga
     1081 cgtacgccgg gatgtcgaaa gggaattcaa gggtcggtat cgttcctgat ctgatgatga
     1141 agccaaggaa actgattctc ctcgccttgc tcgtggccgt ggtgctcggt ccgggcaccg
     1201 ccgttgcggc cgacgagccg accaccacca cggcggttcc gacgaccagc tcggacgctt
     1261 cgaccgacac cgccccgctg acgctcgacc aggatcagat caacgcgctg accgaacagg
     1321 ccaacggcgc cgacaatgac aagagcgtct tcgaaaaggc ttccgatttc gtcaccgaca
     1381 acgccccctg gttcatcatc gggatcatcg tcctggcggc gatcatcgcc ggcatcctga
     1441 tcatgcgggg ccgcccccgg aagggagcta cgcagggcac gccgaaggcc tcgaagtcga
     1501 acaaggcgat gcgcagtgcc ggcgggcttt cgcctgccga ggttgcgtct tcaccggccc
     1561 agaccccaag cccctccgag cttcgccgtc gcaagcgggc ggcgatccag cgttcccgcg
     1621 aagaggaaag ggcccgccgc aaggccggca tcgaaaaccg caaggcggcc cgtcgcggtg
     1681 aggcgccggc cccggtttca cctgccaccg catctgcggc gggcgtcacc gcggtgaccg
     1741 cggccggagt ggccgccgcc ggcgggatgg atccggtcga ggccgagaag cagggtgcca
     1801 gggaccagga agttgccgcc ggagcgatgg cgaggtatgg cgcggttcag gcgccggctg
     1861 cgccggccga gaccgttccc gcgcccgctt tcacctccgg acaggcgatc cctgccccgg
     1921 tttcgccaag ccagactgcg ccggcggccg gtgtggtgac cccggacagc gtgccccctg
     1981 ccggaccagg gacttatgaa tcgccagttc ccgaacccgt aacggagccg gtttcggaat
     2041 acgcagcgga gccggccggc gggtacgccc cggaagtcgc ggaaaccgcc ccggcggcat
     2101 tcgctgcgga tgacacggtc tacgaaccgg ccgccgccac gccggaagcc gaagccgaaa
     2161 ccagcatcat tcacgagcct tcactcgaga accccggcgc cgacgccgcg gttggcaacg
     2221 ccgccgaagc tttcctggct ggcggtgccg ccggagctgc cggcggactg gcggccgccc
     2281 gtgccagccg gcccgaggaa cccgcggcgg agccggtcga gccacttgaa caggtaatcg
     2341 cccccgagcc cgggatggcc ccggaacatg ccctggccga cgaagcgccg gtttcaccgg
     2401 tcgccgggca acaggcacct gacgccgcaa cggcagccgc cgaggaacgc ctgcgggcca
     2461 aggttgcgga gatcaaggcc acccaggagc cgcccgcccc gccgccgccc gtcgagcctc
     2521 cggccgccgg gattgcgccc gagcctgtgc ccccgcggga gggactgccc g
//
LOCUS       DAOQKN010000266         2562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_29350_length_2562_cov_4.198644, whole genome shotgun sequence.
ACCESSION   DAOQKN010000266 DAOQKN010000000
VERSION     DAOQKN010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29350_length_2562_cov_4.198644"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="PLJ64_12230"
     CDS             complement(<1..270)
                     /locus_tag="PLJ64_12230"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC94295.1"
                     /translation="MTSENGTTASPGTERITIGDLADRTGMTVRNIRAHQSRGLLDPP
                     KVVGRTGYYDQGHVDRIELIRELQEEGFNLEAIRRILDSAGEDPGE"
     gene            382..1833
                     /locus_tag="PLJ64_12235"
     CDS             382..1833
                     /locus_tag="PLJ64_12235"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNC94296.1"
                     /translation="MNEQSPPEKLIRSRTDRYVGGVAAGLAAYLKVDPVFVRIAFVAT
                     LALGGLGLLAYVILLAVMPVEGDPAEPLPPIEPKRRNLMIGLAVIVGIIGIATADSGN
                     FAGWLFGFWPGTLFGILFWSAAAVAVIWLLASGALTGNRGDEPAEAPRTPSFPDSEPR
                     KQDPSTEVVTYAESPTQLPTEVMETRQMYARAGQGAGGETSEGSGPEASAASAPPARD
                     DGSGVVGKIMVWFAIGLTALIVFCTLFVISAGTTAIFGGVPMAALVIILGGGMIFAGV
                     RGRRQVSLWLLTAAIAITLPMAAVSIADLRVEGSYGEIRETPLTMADIPEDGYTMAAG
                     NMTIDLRKFHFSPLHEQSIEVKSGMGLTSVIVPDDVCVSGLVTGKAGIANIRGLESNG
                     LDIHQSAPGPSDWKPGMRSRGNLLLIDGDFKLGAFEVVDATQWRRDGRGGSFEDNDLD
                     PDARASTAARARATAACAPRPTRLEKLKATASS"
     gene            1857..>2562
                     /locus_tag="PLJ64_12240"
     CDS             1857..>2562
                     /locus_tag="PLJ64_12240"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNC94297.1"
                     /translation="MRDRQGGMLAGVCSGIALRLKVDPTLVRVVAVVLTVITGGVAAI
                     GYGLLWVALPVAGEEAIVRRFKPRQGSREKVVAKPAPARPFKGSWAVAAGAGFVTLGL
                     LFVFRETGVWWSDSLVWPLVIAAAGAALIWRQFSQSSRTILGPFRGGVGIALLLGGAV
                     LFLYINGALSAGGDVALAGLVAVIVIGLLAAPIWARSARQLSTERAERIRSEERAEVA
                     AHLHDSFLQTLALIQKN"
BASE COUNT          382 a          893 c          842 g          445 t
ORIGIN      
        1 ttcacccggg tcttcacccg cgctgtcgag gatccggcgg atcgcctcga ggttgaaccc
       61 ttcctcctgg agttcgcgga tcagttcgat ccggtcgacg tggccctggt cgtagtagcc
      121 ggtcctgccc accaccttcg gcgggtcgag caggccgcgc gactggtggg cgcggatgtt
      181 gcgcaccgtc atgccggtgc ggtcggccag gtcaccgatc gtgatccgtt cggttcccgg
      241 actcgcagtt gtcccgtttt cggaagtcac ctgagaagat cgtaagccgt aaagggggtc
      301 gggcaccccg ggtcgaccct gatgcgatct aagggtcatt cccaatcgac aggatcggcc
      361 cggccgttca gtctcaaggc catgaacgaa cagtctcctc ccgagaaact catccgctcc
      421 cgcaccgacc gctatgtcgg cggtgtcgcc gcaggcctcg ccgcgtacct gaaggtcgac
      481 ccggtcttcg tccggatcgc cttcgtcgcc accctggccc tgggcgggct cggactcctt
      541 gcctacgtga tcctgctggc cgtgatgccg gtcgaaggcg acccggccga gccgctgccc
      601 ccgatcgagc cgaaacgccg caacctgatg atcggcctcg cggtgatcgt cggcatcatc
      661 ggcatcgcga cggccgattc agggaacttc gccggctggc tgttcggctt ctggcctggc
      721 accctcttcg gcattctctt ctggtcggcg gccgcggtcg ccgtaatctg gctgctggct
      781 tccggagcac tcaccggaaa ccgtggcgac gaacccgccg aggcaccccg aaccccttcc
      841 tttccggatt ccgagcccag aaagcaggac ccgtcgaccg aggtcgttac ctacgccgag
      901 tccccgaccc agcttccaac cgaagtgatg gagacgaggc agatgtacgc acgggccggc
      961 cagggcgcgg gcggcgagac ctccgagggg tccggccccg aagcttcggc cgcctcggcc
     1021 cctccggccc gggatgacgg ttccggtgtg gtcggcaaga tcatggtctg gttcgcgatc
     1081 ggcctgaccg cgctgatcgt cttctgcacc ctcttcgtga tctcggccgg gacgaccgcg
     1141 atcttcggcg gggtgccgat ggctgccctg gtgatcatcc tcggcggcgg catgatcttc
     1201 gccggggtgc gcggccgccg gcaggtgtcg ctctggctct tgaccgccgc gatcgcgatc
     1261 accctgccga tggccgccgt ctcgatcgcc gacctccggg tcgaaggcag ctacggcgaa
     1321 atcagggaaa ccccgctcac gatggccgac atcccggagg acggctacac catggccgcc
     1381 ggaaacatga cgatcgactt gcggaagttt cacttttccc cgcttcacga acagtcgata
     1441 gaggtgaaga gcggcatggg cctgacttcg gtcatcgttc cggacgacgt ctgcgtgagc
     1501 ggcctggtca ccgggaaggc cgggatcgcc aacattcgcg ggcttgaatc gaacggcctc
     1561 gatatccacc agagcgctcc cggcccgtcc gactggaagc ccggcatgcg aagcagaggc
     1621 aacctgctgc tgatcgacgg cgatttcaaa ctcggagcct tcgaggtcgt ggacgcgacc
     1681 cagtggcgtc gggatggccg cggcggcagc ttcgaagaca acgatctcga tcccgatgcg
     1741 cgggcatcga ccgccgcacg cgcacgggcg accgcagctt gcgcacctcg cccgacgcgc
     1801 ctcgagaagc tgaaggcgac ggccagctcc tgaacgaacc acccgacaag cggctcatgc
     1861 gcgaccgcca gggcgggatg ctggccgggg tctgctccgg catcgcgctg cggctcaagg
     1921 tcgacccgac cctggtccgg gttgtcgcgg tggtgttgac cgtgatcacc ggcggggtcg
     1981 cggcgatcgg ctacggcctg ctctgggttg ccctgccggt ggccggcgaa gaggcgatcg
     2041 tccgccgctt caaaccaagg cagggcagcc gggagaaggt cgtcgcaaaa ccggctccgg
     2101 cgcgaccctt caaaggctcc tgggcggttg ccgccggggc cggtttcgtc accctcgggc
     2161 tgctcttcgt cttccgtgag accggggtct ggtggtcgga ctccctggtc tggccgctgg
     2221 tcatcgccgc cgccggggcg gcgctcatct ggcgccagtt cagccagtca tcccgcacca
     2281 tcctcggacc cttccggggc ggggtcggca tcgccctgct gctgggcggg gccgtcctct
     2341 tcctctacat aaacggggct ctcagcgccg gtggcgacgt cgccctggcc ggactggtcg
     2401 cggtgatcgt gatcggtctc ctggccgccc cgatctgggc ccgctcggcc cgccagctca
     2461 gcaccgagag agccgagcgg atccgctccg aggaaagggc cgaagtcgcc gcccacctgc
     2521 atgattcgtt cctccagacc cttgccctga tccagaagaa cg
//
LOCUS       DAOQKN010000267         2509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_30510_length_2509_cov_4.892828, whole genome shotgun sequence.
ACCESSION   DAOQKN010000267 DAOQKN010000000
VERSION     DAOQKN010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30510_length_2509_cov_4.892828"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="PLJ64_12245"
     CDS             complement(<1..959)
                     /locus_tag="PLJ64_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94298.1"
                     /translation="MARPIQVHLSPQPLERYRDLLGPDFDPIAELADGARGRLEGKVV
                     WNVNSTARGGGVAEMLRAYLPYVLDDGVDTRWAVLQEDPSFFHLTKRLHNSFHGDPGD
                     GGPLGESEKRFYVESLADSGRQLAGMVSPDDVVILHDPQTAGLVPAIRDRGATVIWRC
                     HIGVDESNEQTVRAQDFLAAEVGQADACVFSRPSYVWEALPDGISHIIPPGIDAFTPK
                     NSEMDEATRDAVLGVIGLSGEKPAEPPAFTRPDGGQGEVLSSGVVVQTNPLPPAVPLV
                     VQVSRWDKLKDHGGLLTLFSKEMKNRDAHLALVGPDSAGVDDDP"
     gene            1136..>2509
                     /locus_tag="PLJ64_12250"
     CDS             1136..>2509
                     /locus_tag="PLJ64_12250"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC94299.1"
                     /translation="MIGLGTVWILDGLEVTLVGSIGSRLTEPGSGLALGASQIGTAAA
                     IYVAGAVLGALFFGQLTDRFGRKKLFLITLAVYITATIATAFSFSAWYFYLCRFFTGA
                     GIGGEYSAINSAIDELIPARVRGRVDLIINGSYWLGAAAAALGSIVLLDTALFAADVG
                     WRLAFGLGATFGVAILIVRRHVPESPRWLFIHGREKEADRIVSDIEREVERETGRQSL
                     EEPEEIIEVEQRKTISLGEVASTAFRKYPKRTALGLALFVGQAFLYNAITFDLGTLLG
                     TFYGVASGTVPIFIAVYALSNFAGPLVLGRLFDTVGRKPMIVLTYGIAAAGGLALGLL
                     FTQDGFDKYWFMALLVTIFFFASAGASSAYLTVSEIFPMETRALAIAFFYAVGTGLGG
                     IVGPLLFGHLIETGDRSTMAIGFYLGAGIMLIGALAELIFGVKAEKQPLEQIARPLTA
                     TSDQVEAE"
BASE COUNT          415 a          823 c          822 g          449 t
ORIGIN      
        1 gggtcgtcgt cgaccccggc gctgtccggc ccgaccaggg ccaggtgcgc atcccggttc
       61 ttcatctcct tcgagaagag ggtgagcagt ccgccgtgat ccttgagctt gtcccagcgc
      121 gaaacctgaa ccaccagcgg caccgccgga ggcagcggat tggtctgaac caccacgccg
      181 gaactcagca cctcgccctg gccgccgtcg gggcgggtga acgccggcgg ctccgccggc
      241 ttctcgccgg agagcccgat cactccgagg accgcgtccc gcgtcgcttc gtccatctcc
      301 gagttcttcg gggtgaaggc atcgatcccg ggcgggatga tgtgcgagat gccgtccggg
      361 agcgcttccc agacatagga gggccgggag aagacgcagg catccgcctg gccaacctcg
      421 gccgcgagaa aatcctgggc ccggaccgtc tgctcgtttg attcgtcgac cccgatgtgg
      481 cagcgccaga tcacggttgc gccccgatca cggattgccg gcaccagccc ggccgtctgg
      541 gggtcgtgga ggatcaccac gtcatccggt gaaaccatcc cggccagttg ccggccgctg
      601 tcggcgagcg actcgacata gaagcgcttc tctgactctc cgagcggccc gccatcaccg
      661 gggtcgccgt gaaaactgtt gtggagccgc ttggtcagat ggaagaagga cgggtcttcc
      721 tgcaggactg cccagcgggt gtcgaccccg tcgtcgagga cgtagggcag gtaagcccgc
      781 agcatttctg ccacgccgcc gcccctcgcg gtcgagttga cgttccagac gacctttcct
      841 tccagacgcc cgcgggcccc gtcggccagc tcggcgatcg gatcgaaatc aggccccaat
      901 aaatcacggt aacgctcgag gggctgcgga ctgagatgaa cctgtatcgg cctggccatg
      961 cgggcagtca accagaccgg aggcagaacg ggagcggggg tgagtgaagc gggaggaatc
     1021 gggtacatga gccgacatga gttcggagtc gggcacggag gagctgggtc ggatacggac
     1081 caggattccg gcgcgtctgg accggttgcc gtggagccgc tttcactgga tggtggtgat
     1141 cgggctcgga accgtctgga tcctcgacgg gctggaagtc accctggtcg ggagcatcgg
     1201 ctcccgactg accgagcccg gcagcgggct ggccttaggg gccagccaga tcggcaccgc
     1261 cgccgcgatc tacgtcgcgg gagcggtgct cggggcgctc ttcttcggcc agctcaccga
     1321 tcgcttcggc cgcaagaagc tcttcctgat cacgctggcc gtctacatca ccgccaccat
     1381 agctacggcc ttctcgtttt ccgcctggta cttctacctc tgccggttct tcaccggcgc
     1441 cggaatcggc ggtgaatact cagccatcaa ctcggcgatc gacgagctga tcccggcccg
     1501 ggtgcgggga agggtcgacc tgatcatcaa cggcagctac tggctggggg cggcggcagc
     1561 cgcgctgggt tcgatcgttc tgctggacac ggccctgttt gctgccgacg tggggtggag
     1621 gctcgccttc ggcctcggcg ccaccttcgg agtcgcgatc ctgatcgttc gcaggcacgt
     1681 gcccgagagc cctcgctggc tgttcatcca cggacgggag aaggaagccg accggatcgt
     1741 cagcgacatc gaacgggaag tcgaacgcga aaccggccgc cagtcgctgg aggaaccgga
     1801 agagatcatc gaggtggaac aacgcaagac gatctccctc ggggaagtcg cctcgaccgc
     1861 ctttcgcaag tacccgaaac gaacagccct ggggctcgcc ctgttcgtcg gccaggcctt
     1921 tctctacaac gcgatcacct ttgacctcgg gaccctgctc ggcacgttct acggtgttgc
     1981 ctccggcacc gtgccgatct tcatcgcggt ctatgccctg agcaatttcg ccgggccact
     2041 ggttctgggt cgcctctttg acacggtagg ccgcaagccg atgatcgtgc tcacctacgg
     2101 gatcgccgcc gccggcggac ttgccctcgg cctgctgttt acccaggacg gcttcgacaa
     2161 gtactggttc atggcgttgc tggtcacgat cttcttcttc gcctcggccg gggccagctc
     2221 ggcctacctg acggtgagcg agatctttcc gatggagacc cgggccctgg cgatcgcctt
     2281 cttctacgcg gtcggtaccg gcctgggcgg gatcgtcggc ccgttgctct tcggccacct
     2341 gatcgaaacc ggtgaccgga gcacgatggc gatcggcttc tatctcggcg ccggaataat
     2401 gctgatcggt gccctggccg aactgatctt cggggtcaaa gccgaaaagc agccgctaga
     2461 gcagatcgcc aggccgctga ccgccacttc ggatcaggtg gaggccgaa
//
LOCUS       DAOQKN010000268         2509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_30520_length_2509_cov_2.980440, whole genome shotgun sequence.
ACCESSION   DAOQKN010000268 DAOQKN010000000
VERSION     DAOQKN010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30520_length_2509_cov_2.980440"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /locus_tag="PLJ64_12255"
     CDS             complement(<1..1002)
                     /locus_tag="PLJ64_12255"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNC94300.1"
                     /translation="MEANTTTTGSAPATPAPGGGISSSYDAIVAGGGHNRLTTAAYLA
                     RAGMKTLVLERRPILGGACVTEEVWPGARVSRCSYVVSMLQSRVVSDLRLKEFGYRAY
                     PLDPAYAAMTEDGPIFFFNEVERTVESIRRFSPKDADNYEAFEDLLERTAAFLRPMLL
                     REPPALGSKSPGDIASLIREGARVAGMSRRDINDLIRIFTMSVGDLLDDWFELDALKG
                     SIASTGVVGVWAGPRTPGTAYNLLHHALGELDGMGGVWGQVVGGMGAISNAIARSAES
                     HGATILTDAEVASIDVESGRAIAVTLASGDSYRAPVVASGAHPKTTILDLVGGEHFDD
                     "
     gene            1107..>2509
                     /locus_tag="PLJ64_12260"
     CDS             1107..>2509
                     /locus_tag="PLJ64_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNC94301.1"
                     /translation="MTGESSALQSAAKQHLMLHFSDMKSLSEEGMTIIERGEGVHVFD
                     DQGGRYIDGLSGLYCASLGHSHGDEIGAAAHEQMKKLPYATNWTVAHPPSIELAEKIA
                     SLAPEGMNKVYFTSGGSESVEAAWKMAVQWQQANGHERRRKVIARRNAYHGVTMGALS
                     FTGIPVCRTPFDPLGIPTVHVGNTSNYHRPEGETEAEFCAALLAEVEATIEFEDPSTI
                     AMMIAEPVQNAGGCLMPPDGYWQGLRKICDEHDILLCSDEVICAYGRIGTWFGGQKFG
                     YVPDLVTFAKGLTGAHFSMGGVLMSDKVAEPFFDGRADYNNGYTFGGHPVGSAVALKS
                     LEILERENVLENVNSNAPVAEEALKSLLDIPIVGQIRGTGHFWAIEVVRDRETKEPFK
                     GKAAEHLFKNVLSEALWHRGLLCRLDDRADPIIQIAPPLVAEPELFTEIAGILREGLE
                     ITVEQAGAIAPAESSISP"
BASE COUNT          433 a          820 c          860 g          396 t
ORIGIN      
        1 gtcatcgaag tgttcgccac cgaccaggtc aaggatcgtg gtcttcgggt gggcaccgga
       61 ggcgacgacc ggggcccggt agctgtcacc gctggcgagg gtcaccgcga tcgcccggcc
      121 ggactcgaca tcgatcgagg cgacctcggc gtcggtcagg atcgtcgcgc cgtgggattc
      181 ggccgagcgg gcgatcgcgt tcgagatcgc gcccatgccg ccgaccacct ggccccagac
      241 gccgcccatg ccgtcgagtt cgccgagcgc gtggtggagg aggttgtagg cggtgccggg
      301 ggtgcggggc ccggcccaga ccccgaccac gccggtcgag gcgatcgatc ccttcagcgc
      361 gtcaagctcg aaccagtcgt cgagcaggtc gccgaccgac atggtgaaga tccgaatcag
      421 gtcgttgatg tcgcgacggc tcatcccggc aacccgtgcg ccctcccgga tcagcgaggc
      481 gatgtcaccg ggcgatttcg agccgagcgc cggcggttcc ctgagcagca tcggccgaag
      541 gaaggcggcc gtccgttcga gcaggtcttc gaaggcttcg tagttgtcgg cgtccttcgg
      601 ggagaagcgg cggatcgatt cgaccgtccg ctcgacctcg ttgaagaaga agatcgggcc
      661 gtcctcggtc atcgccgcgt aggcggggtc gagcgggtag gccctgtagc cgaattcctt
      721 gaggcgcagg tcggagacga ccctggactg cagcatcgag acgacatagg agcagcgcga
      781 aacccgggcc ccgggccaga cctcttcggt cacgcaggcc ccgccgagga tcggccgccg
      841 ctcgaggacc agggtcttca tgccggcccg ggcgaggtag gccgcggtgg tcaggcggtt
      901 gtggccgccg ccggccacga tcgcgtcgta actcgaggaa attccgccgc ccggggccgg
      961 agtcgcgggg gcgctgccag tggtcgtcgt gttcgcctcc atggcgcgac cttaacgcaa
     1021 agtgcgattg actacaaggt caacactgat tatcaactga taagcagacc ctcgatctgg
     1081 cggccccggg gaatacactc cgggccatga cgggagagag cagtgccctg cagtccgcgg
     1141 cgaaacagca tctgatgctc catttctccg acatgaagtc actgtcggag gagggcatga
     1201 cgatcatcga acggggcgag ggggtccacg tgttcgacga tcagggtggc cgttacatcg
     1261 acgggctgtc cggcctctac tgcgcttcgc tcggccattc ccacggtgac gagatcggcg
     1321 ccgccgccca cgagcagatg aagaagctgc cctacgcgac caactggacg gttgcccatc
     1381 cgccttcgat cgagctggcc gagaagatcg cctcgctcgc gccggagggg atgaacaagg
     1441 tctacttcac ctcgggcggc tcggagtcgg tcgaggcggc ctggaagatg gccgtccagt
     1501 ggcagcaggc caacggccac gagcgccgcc gcaaggtgat tgcccgcagg aacgcctatc
     1561 acggggtgac catgggggcc ctttccttca ccggcatccc ggtctgccgg actcccttcg
     1621 acccgctcgg gatccccacc gtccacgtcg ggaataccag caactatcac cggccggaag
     1681 gggagaccga agcggagttc tgcgccgccc tgctggccga ggtcgaggcg acgatcgagt
     1741 tcgaggaccc ctcgacgatc gcgatgatga tcgccgagcc ggtccagaat gccggcggct
     1801 gcctgatgcc tccggacggc tactggcagg gcctcaggaa gatctgcgac gaacacgaca
     1861 tcctgctctg ctcggacgag gtgatctgcg cctacggccg gatcggcacc tggttcgggg
     1921 gccagaagtt cggctatgtg ccggatctgg tcaccttcgc caaggggctg accggggccc
     1981 atttctcgat gggcggggtg ctgatgagcg acaaggttgc cgaacccttc ttcgacggcc
     2041 gcgccgatta caacaacggc tacaccttcg gcggccatcc ggtcggctcg gcggtggcgc
     2101 tgaagtcact ggagatcctc gagcgggaga acgtgctcga gaacgtgaac tcgaacgccc
     2161 cggtcgccga ggaagcgctg aagtcgctgc ttgacatccc gatcgtcggc cagatccggg
     2221 gcaccggtca tttctgggcg atcgaggtgg tgcgggaccg cgagaccaag gagcccttca
     2281 agggcaaggc ggccgaacac ctcttcaaga acgtcctctc cgaggccctc tggcaccggg
     2341 gcctgctctg ccggctcgat gaccgggccg acccgatcat ccagatcgcc ccgcccctgg
     2401 tcgccgagcc ggagctcttc acggagatcg ccggaatcct ccgcgagggc ctggagatca
     2461 cggtcgagca ggccggcgcg atcgctcccg ccgagagttc gatcagccc
//
LOCUS       DAOQKN010000269         2484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_31066_length_2484_cov_4.594072, whole genome shotgun sequence.
ACCESSION   DAOQKN010000269 DAOQKN010000000
VERSION     DAOQKN010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31066_length_2484_cov_4.594072"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2131
                     /locus_tag="PLJ64_12265"
     CDS             <1..2131
                     /locus_tag="PLJ64_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94302.1"
                     /translation="LNGESRAYLLTPTQPLKATLQPVTVGSNDVFDLKMTVQHLGGAG
                     ATEISNLSYPPAPGLVLRTVPVLGGPAPTLTRIFGPTGTFPTSLDPGQKSEHVFAYAI
                     DSPGNGLLQTRVKGTDQEGNEYDASAALLIESELKQPDRQDLDSMIAGMLLQLADQAY
                     RKRNALARELFDIVKKRLRGTPASMRKPTRFEQAAAQAYGLPGSALSAFPNGAKARRT
                     ALGSFMGAVSEEMETVGSEAIDRSVVVPFSYWKDWLFSPDENKGRMALEMADTLSEGA
                     QGMGGVLGGAADYYTSPSQWKATWDDLPEMYAESTAALKKVDVSLSNAILNWDDTMKK
                     DPAEGARQFGKLLGRIEGEVAVGWLEEFTGGKALQGAKYIRDTRIAGGVATETAEVAT
                     SASRLTGAGAKALPDAPSLGNLSNRQIDRFKQIVKNVNEKFGVKVEIQARPINEYAAK
                     VKGGIGKVEAVPTKNLTPDDVILGAPPEWVGQAGYYPPKLPKNFNKLDAAEKARLQLR
                     FDEKTKEFKQFKGVIEDPTGKTAKVHKALKKETEFDLGKNGKLKMELEATTHKSGAVP
                     ISYKKLSVNGKPVFTGKPRPIISDVDMNAVVDAATGRHLPAGIRGQVELEVMRQFAKA
                     AEEGVFPFGYHGWTHSGFDIAAADFRHILKYQLMYMNEKDAVKLAKKYAAIYGIDPSE
                     FLDGYTRGKLLVKITATDAVLGPGAGL"
     gene            complement(2197..>2484)
                     /locus_tag="PLJ64_12270"
     CDS             complement(2197..>2484)
                     /locus_tag="PLJ64_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC94303.1"
                     /translation="SQIERFAPGFRDRIRGETAMYPEDFAQYNANYVGGDIVAGANTP
                     RQMVFRPRVTLNPYKVTEGIWICSAATPPGGGVHGMGGANAARSILSTLGS"
BASE COUNT          505 a          813 c          780 g          386 t
ORIGIN      
        1 cctcaacggc gagagccggg cctatctgct cacgccgacc cagcccctga aggcaacgct
       61 tcagccggtc acggtcggca gcaatgacgt cttcgacctg aagatgactg tccagcacct
      121 gggcggcgcc ggcgccaccg aaatcagcaa cctgagttat cccccggccc cgggccttgt
      181 gctccgcacg gtgccggtcc tcggcggccc ggcgcccaca ctcacccgga tctttggtcc
      241 gaccggaacc tttccgacca gcctcgaccc ggggcagaag tccgaacatg tcttcgccta
      301 cgcgatcgat tcacccggca acggcctgct tcagaccaga gtcaagggca ccgaccagga
      361 agggaacgag tacgacgcgt cggccgccct gctgatcgag tccgagctca agcagcccga
      421 ccggcaggac ctcgacagca tgatcgccgg catgctgctt cagcttgccg accaggccta
      481 tcggaagcgg aatgcgctgg cgagggaact gttcgacatc gtcaagaagc gcctgcgcgg
      541 caccccggca tcgatgcgga agccgacccg gttcgagcag gccgcggccc aggcatatgg
      601 tttgccgggc tcggcccttt cggcgtttcc gaacggagcc aaggcccggc gtactgcgct
      661 cggttcgttc atgggtgcgg tttccgagga gatggagacg gtcgggtcgg aggcgatcga
      721 ccggtcggtc gtggttccct tctcgtactg gaaggactgg ctcttctcgc cagacgagaa
      781 caaaggtcga atggcgctgg agatggccga caccctctcg gaaggcgccc aggggatggg
      841 cggcgttctg ggaggagctg ccgactacta cacctcgcct tcgcagtgga aggcgacctg
      901 ggatgacctg cccgagatgt acgcagaatc cacagcggcc ctgaaaaagg tcgatgtgag
      961 cctctcgaac gccatcctga actgggacga cacgatgaag aaggatccgg ccgagggagc
     1021 ccgccagttc ggaaaactgc ttggccggat cgaaggcgaa gtggcggtcg gctggctcga
     1081 ggagttcacc ggcggcaagg ccctgcaggg ggcgaagtac attcgggata cgcggatcgc
     1141 cggcggtgtc gccacggaaa ccgccgaggt cgcgaccagc gccagccgtc tcaccggcgc
     1201 gggtgccaag gccctcccgg acgcgccttc gctcggcaac ctcagcaacc ggcagatcga
     1261 ccgcttcaag caaatcgtca agaacgtcaa cgagaagttc ggggtcaagg tcgaaatcca
     1321 ggcccggccg atcaacgagt acgccgccaa agtcaagggc ggaatcggca aggtcgaggc
     1381 ggtcccgacc aagaatctga ccccggacga cgtgatcctc ggggctccgc ccgaatgggt
     1441 cggccaggcc ggttactacc cgccgaagct cccgaagaac ttcaacaaac tcgacgcggc
     1501 cgagaaagcc cgcctccagc tccgctttga cgaaaagacg aaggagttca agcagttcaa
     1561 gggcgtcatc gaggatccca cgggcaagac ggcaaaggtt cacaaggccc tgaaaaagga
     1621 aaccgagttc gatctgggca agaacggcaa gctgaagatg gaactcgaag cgaccaccca
     1681 caagtccggg gcggtgccga tcagctacaa gaagctctcg gtcaacggca agccggtctt
     1741 caccggcaag cccaggccga tcatctccga cgtggacatg aacgcagtgg ttgatgcggc
     1801 caccggccgt cacctgcccg ccggcattcg cggccaggtc gagctggagg tgatgcgcca
     1861 attcgccaag gccgccgagg aaggtgtctt ccccttcggt taccacggct ggacccactc
     1921 cggattcgac atcgccgccg ccgacttccg tcacatcctc aagtaccagc tcatgtacat
     1981 gaacgagaaa gacgcggtca agctcgcgaa gaagtacgcc gcgatctacg gcatcgaccc
     2041 ttccgagttc ctcgacggct acacccgcgg caaactcctg gtcaagatca ccgccaccga
     2101 cgccgtcctc ggcccgggcg ccggccttta ggctgaacct gcatctacgt tgaagttatc
     2161 cgcacatgga acgaataact tcaacccaga tgcttctcag ctaccgagcg tgctgaggat
     2221 cgagcgggcc gcgttggctc cgcccatgcc atggactccc ccgccgggcg gggtcgcggc
     2281 ggagcagatc cagattccct cggtgacctt gtacgggttc agcgtgaccc ggggccggaa
     2341 gaccatctgc ctcggggtgt tcgccccggc gacgatgtcg ccgcccacgt aattggcgtt
     2401 gtactgggcg aaatcctcgg ggtacatggc tgtctccccg cggatccggt cgcggaaacc
     2461 gggtgcgaag cgttcgatct ggct
//
LOCUS       DAOQKN010000270         2472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_31365_length_2472_cov_3.707489, whole genome shotgun sequence.
ACCESSION   DAOQKN010000270 DAOQKN010000000
VERSION     DAOQKN010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31365_length_2472_cov_3.707489"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="PLJ64_12275"
     CDS             complement(<1..281)
                     /locus_tag="PLJ64_12275"
                     /inference="COORDINATES: protein motif:HMM:NF039728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6325 family protein"
                     /protein_id="HNC94304.1"
                     /translation="MTEEIEEMGPIDYILVEWPNNEPTGEAIPYLVDLVDSGLIRILD
                     LAFIMKNEDGSVTEMEISDLGGESESFTVFEGASSGILDESDHAEAAEAL"
     gene            complement(366..>2472)
                     /locus_tag="PLJ64_12280"
     CDS             complement(366..>2472)
                     /locus_tag="PLJ64_12280"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNC94305.1"
                     /translation="AVAVCSEAEAELLEAPAGYSKTTTLRLWDEADKRPFAWVRCETR
                     YNDPALLANVIVSSLGETSIDPGLHELLRSPVPDLNLLLERVASAIRGLDPFVLVIDD
                     AHLLESDDAWQVLYSVISALPEGSQVAVATRVRPGLPVGRLRARGELFEFGMNDLALT
                     RGESRELLAGLDLDLGERADEIHEKSEGWPAALYLAGLAIRQGSEARPETVEFDGGDR
                     VVVEYFRDEFIKDMPQEKARFLMETAILEELTGPLCDAVTGRRDSAEMLNELAGENTL
                     VVPLDRNNSRFRYHHLFRDMLRSEMNLRDPGAKPGLHRRAAVWLAANGDIPRATDHAI
                     AAGDFELAGACIWLDLPDLLSRGRVATIDRWFETIGDDRIAMIPGLLLARAHREIAMG
                     RGDETFYWLSVAEREIAEDSPLFGDVLTLRATTGINGARGMIADGERAAGLMSPTSPF
                     QVAAKLYVGIGLGLTDQENASAVLRESAREAAMISGIIQAIALTELALLELDSGRVDA
                     AHDYASRARDVVERSGLRILGVMSLVYVVLALTKAERGRVEEAIVDLETSSRIGEGIL
                     DYIVWHEAQLVLCRCRTLIRIGQFAQAESELGRARIAIGRVPDSPFLERWLEECEGEL
                     EMVRGSGGTTAVSLTRAELRTLQFLPSHHSFRSIGEQLYLSQNTVKTQANSLYRKLGV
                     NSRAEAVIEGRRLGLLKGE"
BASE COUNT          409 a          830 c          777 g          456 t
ORIGIN      
        1 agggcttcgg ctgcttcggc gtggtcggat tcatcgagga tgcccgaaga ggcgccctcg
       61 aacacggtga acgattcgga ttcgccgccg aggtcgctga tctccatctc ggtgacgctt
      121 ccgtcttcgt tcttcatgat gaaggcgagg tcgaggatcc ggatcagtcc gctgtcgacc
      181 agatcgacca ggtaggggat tgcttcaccg gtcggctcgt tgttcggcca ctcgaccagg
      241 atgtagtcga tcggccccat ttcttcaatt tcttcggtca ctgctctgtc cttcctcgaa
      301 ttcaactggg atttgaacga aatctagcca gaaccggttc cgggccatca tccattcggg
      361 gttaatcact cgcccttgag caatccgagt cgccggcctt cgatcactgc ctcggcccgc
      421 gagttcacgc cgagcttgcg gtagagggag ttggcttggg tcttcacggt gttctgggag
      481 aggtaaagct gctcgccgat cgagcggaac gagtggtggc tcggcaggaa ctggagggtc
      541 ctcaactcgg ccctggtcag gctgacggcc gtggtcccgc cgctgccccg caccatctcg
      601 agctcacctt cgcattcctc gagccagcgt tcgaggaatg gtgaatcggg aacccggccg
      661 atcgcgatcc gggcccggcc gagctcgctt tccgcctggg caaactgtcc gatccggatc
      721 agggtccggc agcggcagag gaccagttgg gcctcatgcc agacgatgta atcgagtatc
      781 ccttcgccga tcctagaact ggtctcgaga tccacgatgg cctcttcgac ccggccgcgt
      841 tctgccttgg tcagggccag cacgacatag accagtgaca tcaccccgag gatccgcaat
      901 ccgcttcgtt cgaccacgtc ccgggcccgg gaagcgtagt cgtgggcggc atcgacgcgg
      961 ccgctgtcga gctccagcag ggccagctcg gtcagggcga tggcctggat gattcccgag
     1021 atcatcgcgg cctccctggc gctctccctc agcaccgcgc tggcgttctc ctggtcggtc
     1081 aggccgagcc cgatacccac atagagcttg gccgcgacct ggaagggact ggtcggactc
     1141 atcagcccgg ccgcccgttc cccgtcggca atcattccgc gggcaccgtt gatccccgtg
     1201 gttgcccgga gcgtgagcac gtcaccaaag agaggtgagt cttcagcgat ctccctttcc
     1261 gcgactgaca gccagtagaa ggtctcgtca ccgcggccca tcgcgatttc gcggtgggca
     1321 cgggccagca agaggcccgg gatcatggcg atccggtcgt ccccgatcgt ttcgaaccag
     1381 cgatcgatgg tagcgacccg cccacgactg agcaggtccg gcaggtcaag ccagatgcag
     1441 gccccggcga gttcgaagtc cccggcggcg atcgcgtggt cggtcgccct cggaatgtca
     1501 ccgttcgcgg ccagccagac ggcggcccgg cggtgcagcc cgggtttcgc gccggggtcc
     1561 cgcaggttca tctccgaccg gagcatgtcg cggaagaggt ggtggtaacg gaagcgcgaa
     1621 ttgttcctgt ccagcggcac cacgagcgtg ttctcgccgg ccagctcatt cagcatctcc
     1681 gccgaatccc tgcggccggt aaccgcgtcg caaagcggtc cggtcagctc ctcgaggatt
     1741 gccgtctcca tcaggaacct cgccttttcc tgtggcatgt ccttgatgaa ctcgtcgcgg
     1801 aagtactcga ccacgacccg gtccccgccg tcgaactcga ccgtctccgg acgggcctcg
     1861 ctgccctgcc ggatcgccag accggcgaga tagagcgccg ccggccagcc ctcggatttc
     1921 tcgtggatct cgtccgcccg ctcgccgaga tccagatcga gtccggccag cagctcacgg
     1981 ctttctcccc gggtcagggc aaggtcgttc atcccgaatt cgaagagctc gcccctcgcc
     2041 ctcaaccggc cgaccggcag tcccgggcgg accctcgtcg ccaccgccac ctgggaaccc
     2101 tcgggcaggg ccgaaatgac cgaatagagc acctgccagg cgtcgtcgga ttcgagtaga
     2161 tgggcatcgt cgattaccag cacgaaggga tcgagcccgc ggatcgcgct ggcgactcgc
     2221 tcgagcagga gattcaggtc cgggaccggc gagcgcagca actcatggag tccggggtcg
     2281 atgctggtct cccccagcga cgacacgatg acgttggcga gcagggcggg gtcgttgtaa
     2341 cgggtctcgc agcggaccca ggcgaagggt cgcttgtccg cctcgtccca gagcctcagc
     2401 gtggtcgtct tcgagtatcc ggccggcgcc tcaagcaact ctgcctccgc ttcgctgcaa
     2461 acggccacgg cg
//
LOCUS       DAOQKN010000271         2468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_31451_length_2468_cov_6.078326, whole genome shotgun sequence.
ACCESSION   DAOQKN010000271 DAOQKN010000000
VERSION     DAOQKN010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31451_length_2468_cov_6.078326"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1258
                     /locus_tag="PLJ64_12285"
     CDS             <1..1258
                     /locus_tag="PLJ64_12285"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNC94306.1"
                     /translation="DAEPEGPTRLRLDEILPTAEELAAALRAESSCDRAEIAGSVRRL
                     AETCHDIDLIAASDSRKELAAAIAEHELVAEHGNPNETGIRLTTNSGIGVDVRIVEPG
                     AFGHLLQHFTGSGPHNAELRERAVAQGLHVSENGIKDDETGETEFFATEEEIYERLGY
                     QYIPPELREDRGELDAAARGELPELIERGQIKGDLHSHTTLSDGVASLDEMAAAAEAL
                     GYEYLAITDHSESHGFGNHVEADRLWQRIEEIAELNNEDRKIRLLSGSEVNIHPNGSV
                     DYEDDLLETLDWVIASIHTAFSDDAKKNTDRMITAIENPLVDCIGHPTGRLINQRDPY
                     PLDMERVIAAAVENDTLIEINSNPRRRDLNEIHARMAAEAGVGIVINTDAHRPESLDF
                     IEYGIATARRAWLTADQVRNTGPWWK"
     gene            complement(1329..2249)
                     /locus_tag="PLJ64_12290"
     CDS             complement(1329..2249)
                     /locus_tag="PLJ64_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HNC94307.1"
                     /translation="MADGWSSPNYDEWRETCDTLHAHTQFLGKLCVALAPPEPQLQHA
                     ALRLTARGWETLPLPSPNGNGAFVAALDLRTHHLLIEHSDGMGADIALAPNRPVGEVV
                     REGLTAIDELAGPVEIDPAPQEVDWTAPLDEDDEHRIYDPVQVKAYFEMATAAGMALT
                     RFRAPFRGRSTPLNAWWGSFDLAVNLFSGRPAEPPSQDFIMRNAMDAQEVAVGWWPGD
                     GRYGKPAFYAYAHPAEDGFAGRDLSPVPGGWNEELGEYVLDHEEAAATGNPEQAVIDF
                     CGEIFSHACSVCDWNPALAASAAGDPPPVR"
BASE COUNT          466 a          845 c          799 g          358 t
ORIGIN      
        1 cgacgccgaa ccggaaggcc cgacccggct gcgtctcgat gagatcctcc cgaccgcgga
       61 ggagctcgcc gccgcgctca gagcggagtc gagctgtgac agggccgaga tcgcgggctc
      121 cgtccgccgg cttgccgaga cctgccatga catcgacctg atcgccgctt ccgactcacg
      181 caaggagctg gccgccgcga tcgccgaaca cgaactggtc gccgagcacg gcaatccgaa
      241 cgagaccggg atcaggttga ccaccaactc cggaatcgga gtggacgtgc ggatcgtcga
      301 gcccggtgcc ttcggccacc tgctccagca cttcaccggc tccggccccc ataacgccga
      361 gctccgcgaa cgggcagtcg cccaggggct gcatgtgtcc gagaacggga tcaaggatga
      421 cgagacgggt gaaacggagt tcttcgcgac cgaggaagag atctacgagc gcctcggcta
      481 ccagtacatc ccgcccgaac tccgcgagga ccggggggaa ctcgacgccg ccgccagggg
      541 cgagttgccc gagctgatcg agcggggcca gatcaagggt gacctccact cccacaccac
      601 cctctccgat ggcgtcgcct cacttgacga gatggccgcg gcagccgaag cgctcggcta
      661 cgagtacctg gccatcaccg accactccga atcccatggc ttcggcaatc acgtcgaagc
      721 cgaccggctc tggcaacgga tcgaagagat cgccgagctc aacaacgaag accgcaagat
      781 ccggctgcta tccggatccg aggtgaacat ccatccgaac ggttcggtcg attacgagga
      841 cgacctgctc gagaccctgg actgggtgat cgcctcgatc cacaccgcct tcagcgatga
      901 cgcgaagaag aacaccgacc gcatgatcac cgcgatcgag aacccgctgg tcgactgcat
      961 cggccatccg accggccgcc tgatcaacca gcgagacccc tatcccctcg acatggagag
     1021 ggtgatcgcc gccgcggtcg agaacgacac cctgatcgag atcaactcca atccccgccg
     1081 ccgcgacctg aacgagatcc acgcccgcat ggcggccgag gcgggagtcg ggatcgtgat
     1141 caacaccgac gcccaccggc ccgaatccct cgatttcatc gagtacggga tcgcgaccgc
     1201 ccgccgggcc tggctgaccg ccgaccaggt ccggaacacc ggcccctggt ggaagtagcg
     1261 aggtcggtca agacccacga ttttccacaa atactgtgga aaaccgtggc ttctgatgcg
     1321 aatcggagct agcgaaccgg gggaggatca ccggcggcgc tggcggccag ggccgggttc
     1381 cagtcgcaga cgctgcaggc gtggctgaag atctcgccgc agaagtcgat caccgcctgc
     1441 tccgggtttc cggtcgcggc ggcctcctca tggtcgagca cgtactcgcc cagctcttcg
     1501 ttccacccgc ccggtaccgg tgaaaggtcc cggccggcga agccgtcctc ggccggatgg
     1561 gcatacgcgt agaaggccgg cttgccgtag cggccgtcac cgggccacca gcccaccgcg
     1621 acttcctggg catccattgc gttccgcatg atgaagtcct gcgagggtgg ttccgcgggc
     1681 cggcccgaga agagattcac ggcgaggtcg aaggaacccc accaggcatt gagcggagtc
     1741 gagcggccgc ggaagggagc gcggaaacgg gtcagggcca ttccggccgc ggtggccatc
     1801 tcgaaatagg ccttgacctg cacggggtca tagatccggt gctcgtcgtc ctcgtccagc
     1861 ggcgccgtcc agtccacttc ctggggagcc ggatcgatct ccaccggacc agccaattca
     1921 tcgatcgcgg tcagcccttc ccggacgacc tcgccgaccg ggcgattcgg ggcgagggcg
     1981 atgtccgcac ccatgccgtc ggaatgctcg atcagcaggt ggtgggtgcg aaggtcgagc
     2041 gccgccacga aggctccgtt gccgttcggg gacggcagcg gcagagtctc ccaacccctt
     2101 gcggtaagcc ttagcgccgc atgctgcagc tgcggctcgg ggggagccag ggcgacgcag
     2161 agcttgccga ggaactgcgt gtgggcgtgg agggtgtcgc aggtctcgcg ccactcgtcg
     2221 tagttggggg aactccatcc gtcagccatg cttcgacctt actccccccg aaatcgggct
     2281 ggacgaacca gcatcacctc gggtcccgcc gaaggtctca gaaggcgcgg aagccggggc
     2341 tacggaccag cttgaccggc ttgagcggcg aggccgattc gaccaggaag cgcacgccgt
     2401 cccgtccctc gatcgccagg actgcggcct cgcggtttcg ccaggccaaa gcgaccagca
     2461 gggcgtag
//
LOCUS       DAOQKN010000272         2467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_31485_length_2467_cov_3.516998, whole genome shotgun sequence.
ACCESSION   DAOQKN010000272 DAOQKN010000000
VERSION     DAOQKN010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31485_length_2467_cov_3.516998"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1269
                     /locus_tag="PLJ64_12295"
     CDS             <1..1269
                     /locus_tag="PLJ64_12295"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNC94308.1"
                     /translation="VALAVVVFGTLIGSYLGIHGVLEDAATNWFGLQGFEYLDLARLW
                     QVLLVVGLIVWVYMLFRVMRSRLRREHPGNMPWLFFLAACAIPAFYAVGLLARTAEDF
                     TITEFWRFWVVHLWVEDFLEIFTTVMVAYMFVLLGVVREKVALMVILLDIGLYSVGGV
                     VGTMHHLYFSGTPSEHMALGAFFSAIEVVPLTFLTVEAWSFLQLGANQESKSTSDFPH
                     RWAVMFLVAVGFWNFLGAGVFGFLVNLPIVSYFEIGTALTANHAHAAMMGVYGMLAIG
                     LSLFCLRYMIPSKKWPEKWAKVSFWGTNIGLAWMCFATLLPLGIMQLYKSVDDGYFEA
                     RQLDFLTDKTTEMLEWMRLPGDVVFIVFGAIPVLYMCFLGVKYTIARRGEDGDGRGEA
                     DAELFTVISGTSMEDEPPEISGRPEPTPGT"
     gene            1266..1817
                     /locus_tag="PLJ64_12300"
     CDS             1266..1817
                     /locus_tag="PLJ64_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94309.1"
                     /translation="MNTEVLLAVGYAALLLGGALLLEWLSRHTHNRSQAWRTSGFDYD
                     AQHDFWVCHKGEQLWPTEFDREKRLVRYRAKATVCNACPAKVDCTDSHHGREVTRAVD
                     SWPHSEAGRFHRAIAVMLVVLAVFELAVVLVRNHEPSQIAVLLPLFLLALAVTWWLAV
                     DLIRTPSNFPEAKPAHGNRIGAK"
     gene            1814..2251
                     /locus_tag="PLJ64_12305"
     CDS             1814..2251
                     /locus_tag="PLJ64_12305"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNC94310.1"
                     /translation="MKRSEALQPLSREHHHALYLAKVIRDNGGGGDSPERFLSFWREE
                     GAIHFRIEEEVLLPGSGLAGPGQDEEVARMLDDHLEIRRAAARIEAGEAGPDELVEVA
                     ERLTAHVRFEERELFPRVERELSSEQLRELATAIEAAEQAAET"
     gene            complement(2271..>2467)
                     /locus_tag="PLJ64_12310"
     CDS             complement(2271..>2467)
                     /locus_tag="PLJ64_12310"
                     /inference="COORDINATES: protein motif:HMM:NF012588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94311.1"
                     /translation="TYGGGTALPTQSECLKVLGCDGPGKVNKLAEIIAATVLCGDLSL
                     GSAVVADEWVEAHDELGRNR"
BASE COUNT          377 a          796 c          803 g          491 t
ORIGIN      
        1 gtcgccctgg cggtcgtcgt cttcggcacc ctgatcggtt cctatctggg gatccacggt
       61 gtgctggagg atgccgcgac caactggttc gggcttcagg gattcgagta cctcgacctc
      121 gcccgcctct ggcaggtgct gctggtggtc ggcctgatcg tctgggtcta catgctcttc
      181 cgggtgatgc gctcccggct ccggcgtgag catcccggca acatgccctg gcttttcttc
      241 ctcgccgcct gcgcaatccc ggcgttttac gcggtcggcc tcctggcccg gaccgccgag
      301 gacttcacga tcaccgagtt ctggcgcttc tgggtggtcc acctctgggt cgaggacttc
      361 ctcgagatct tcacgacggt gatggtcgcc tacatgttcg tgctgctcgg ggtggtgcgc
      421 gagaaggtgg cgctgatggt gatcctgctg gacatcggcc tctattcggt cggcggcgtg
      481 gtgggcacga tgcaccacct ctacttctcc gggaccccgt ccgagcacat ggccctcggc
      541 gcgttcttct cggcgatcga ggtcgtaccg ctcaccttcc tcacggtcga agcctggtcg
      601 ttcctgcagc tcggtgcgaa ccaggaatcg aagtcgacct ccgacttccc gcaccgctgg
      661 gcggtcatgt tcctggtcgc ggtcggtttc tggaacttcc tcggggccgg cgtcttcggc
      721 ttcctggtca acctgccgat cgtctcctac ttcgagatcg gcaccgccct taccgccaat
      781 cacgcccatg cggcgatgat gggcgtctac ggcatgctgg cgatcggcct ctccctgttc
      841 tgtctcaggt acatgatccc ctcgaagaag tggccggaga aatgggccaa ggtcagtttc
      901 tggggtacca acatcgggct cgcctggatg tgctttgcga ccctgctgcc gctggggatc
      961 atgcagctct acaagtcggt ggacgacggc tatttcgagg cccgccagct cgacttcctg
     1021 accgacaaga cgaccgagat gctcgagtgg atgcggctgc ccggtgacgt ggtattcatc
     1081 gtcttcggtg ccattcccgt gctctacatg tgtttcctcg gagtcaagta cacgattgcc
     1141 cgtcgcggcg aagacgggga cggccggggt gaagcggacg ccgaattgtt cacggtgatc
     1201 tccggcactt ccatggaaga cgaaccgccg gagatctccg gccggccgga accgaccccg
     1261 ggaacgtgaa caccgaggtc ctcctggcag tcggatacgc ggcccttctg ctgggagggg
     1321 ctctgcttct cgagtggctt tcccgccaca ctcacaaccg gtcgcaagcc tggcggacct
     1381 ccggcttcga ttacgacgcc cagcatgact tctgggtctg ccacaaaggg gaacagctct
     1441 ggccgaccga gttcgaccgg gaaaagcggc tggtcaggta ccgggccaag gcgacggtgt
     1501 gcaatgcctg cccggccaaa gtggactgca ccgattccca tcacggccgc gaggtcaccc
     1561 gtgcggttga ttcctggccg cattcggaag ccggccggtt tcatcgggcg atcgcggtga
     1621 tgctggttgt cctggctgtg ttcgaactgg ccgttgtgct ggtccgcaac cacgagcctt
     1681 cacagatcgc ggtgctcttg ccgctgttcc tgctcgccct cgcggtgacc tggtggctgg
     1741 ccgttgacct gattcgcacc ccgtcgaact tccccgaggc gaaacccgcc cacggaaatc
     1801 gcatcggtgc gaaatgaagc ggtccgaggc acttcagccc ctgtcacgcg agcatcacca
     1861 cgcgctctac ctggccaaag tgatccgcga caacgggggc ggaggggatt caccggagcg
     1921 tttcctcagt ttctggaggg aggaaggggc gatccatttc cggatcgaag aggaagtgct
     1981 cctgccgggg tccgggctcg ccgggcccgg gcaggacgag gaagtcgccc gcatgctcga
     2041 cgatcacctt gagattcgcc gggcggcggc ccggatcgag gccggcgagg ccgggccgga
     2101 cgagttggtc gaagtcgccg agaggctcac cgctcacgtc cgtttcgagg agcgggaact
     2161 gttcccgcgg gtcgaacggg agctctcgag cgagcagctt cgggaactcg cgaccgctat
     2221 cgaggcggcc gagcaggcgg cggaaacctg agtcgccggg ggcgcttgcc ctagcggttg
     2281 cggccgagtt cgtcgtgggc ttcgacccac tcgtcggcga ccaccgccga gccgagtgaa
     2341 aggtctccgc aaagcacggt cgcggcaatg atctcggcca gcttgttgac cttgcccggg
     2401 ccgtcgcaac ccagaacctt caggcattcg ctctgggtcg gcagcgctgt gccgccacca
     2461 taggtgg
//
LOCUS       DAOQKN010000273         2396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_33189_length_2396_cov_3.035455, whole genome shotgun sequence.
ACCESSION   DAOQKN010000273 DAOQKN010000000
VERSION     DAOQKN010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33189_length_2396_cov_3.035455"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="PLJ64_12315"
     CDS             <1..246
                     /locus_tag="PLJ64_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94312.1"
                     /translation="RQVLIGLESPNRAGLDGLERRANWKLRQLDRYEAGVRRIQAHGV
                     TVNGCFILGLDGDTEAVFDAIYDFVARTNLFEVHRVP"
     gene            356..1747
                     /locus_tag="PLJ64_12320"
     CDS             356..1747
                     /locus_tag="PLJ64_12320"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNC94313.1"
                     /translation="MEIRNPEGTELLGEVELTDPAEVAAVVGRAVKARRAWTEMPIGR
                     RADLLDAAAALLEPEVEPLGRLLASESGKPIGQATNEVRGGLHFLRGNATEGRRLVGR
                     MLPSESNARSEWDLAFTRREPLGVVAAILPFNFPVELFVEKCAAALVGGNAVVVKPPI
                     EDPLVVGRFREALIEVGVPAEVIAAVHGDAKVGAALAEAPGVDAISLTGSTAAGIAVA
                     RATAEKLRFLHLELGGNNASLILEDADLDLVAAEMLRGRVLMNGQACSASKRVIVHRS
                     LHDELVARLAEAVAAQRVGAATDDTTTIGPLIHEGAATRVAVQVANARSQGAEIAAGT
                     GESEGAYFAPTLLAGVPHTADIAIDDEIFGPVFNLIPVDSEEQALKVANSSSFGLMSS
                     VFTADYRRALIVAERLESGGVVINGTDNYRPPEFPFGGVKLSGRGREGIGYTLEELTR
                     EKPIVFRGFRERS"
     gene            1755..>2396
                     /locus_tag="PLJ64_12325"
     CDS             1755..>2396
                     /locus_tag="PLJ64_12325"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HNC94314.1"
                     /translation="MGAKPDGKYQVPAVVGAISVLNELAGTGEAGATQSELVEATGLS
                     KSTMHNLLSTLDSHGFVRRDNGSRQYRLGPALIPLGNEATRQVKLVRTTLDRVAPLAA
                     EYGLSFAVAQPIDPDELRIISRFYPPGVHVGITVGSAYGPMDGALGKILLSSKEEAVA
                     RKLIKGRKLPAHTRATITSPESLIEEIAEVRTRGWSVSHGEFNENNAVAAGIWG"
BASE COUNT          393 a          801 c          833 g          369 t
ORIGIN      
        1 cggcaagtgc tcatcgggct cgaaagcccg aatcgcgccg gcttggacgg cctggagcga
       61 cgcgccaact ggaagctacg gcagctcgac cgctacgagg ccggtgtacg gcgcatccag
      121 gcgcacggcg tgaccgtcaa cggctgcttt atcctcgggt tggacggcga tacggaagcg
      181 gtcttcgacg cgatttatga cttcgtcgcg cgcaccaacc tgttcgaggt gcatcgcgtg
      241 ccctgagggg accaggggct ccgcgcaggg cggccaggaa atcaattcgg tgacttgaac
      301 cgggttctgt aggctgaacc tcgatgccag cgatcgaggt gagaatttga acacggtgga
      361 aatcaggaat ccggaaggga ccgagctgct cggcgaggtc gaattgaccg acccggccga
      421 agtcgccgcg gtcgtgggcc gcgccgtcaa ggcccggcgg gcctggaccg agatgccgat
      481 cggccggcga gccgacctgc tcgacgccgc ggccgcgctg cttgaaccgg aggtcgagcc
      541 gctcggcagg ttgcttgcct ccgagtccgg caagccgatc ggccaggcaa ccaatgaggt
      601 tcgtggcggt ctccacttcc tgcgtggcaa cgcgaccgag ggtcggcgtc tggtcggaag
      661 gatgcttccc tccgagtcca acgcccggtc cgagtgggac ctcgccttca cccggcgcga
      721 gccgctcggc gtggtcgccg cgatcctgcc gttcaacttc ccggtcgagc tcttcgtcga
      781 gaagtgcgcc gccgccctgg tcggcggcaa cgccgtggtg gtcaagccgc cgatcgagga
      841 tccgctggta gtcggccgtt ttcgcgaggc gctgatcgag gtcggagttc ccgctgaagt
      901 gatcgcggca gttcacgggg acgcgaaggt cggggcggcg ctcgccgaag cccccggggt
      961 tgacgcgatc tccctgaccg gctcaaccgc cgccggcatc gcggtcgccc gcgccacggc
     1021 ggagaaactt cgctttctcc atctggagct agggggaaac aacgcctcgc tgatcctcga
     1081 agacgcggac ctggatctcg tcgccgccga gatgctgcgg ggccgggtgc tgatgaacgg
     1141 ccaggcctgc tcggccagca agcgggtgat tgtccaccgc tcgcttcacg acgaactggt
     1201 cgcccggctg gccgaggcgg tcgccgccca gagagtcggt gccgcaaccg acgacacgac
     1261 cacgatcggc ccgctgatcc acgaaggcgc cgccacgagg gtcgccgtcc aggtcgcgaa
     1321 cgcccgctcc cagggggcgg agattgcggc tggcacgggc gagtcggagg gtgcctactt
     1381 cgctccgacc ctgctggccg gggttccgca caccgccgac atcgcgatcg acgacgagat
     1441 cttcgggccg gtcttcaacc tgatcccggt cgactccgaa gagcaggcgc tcaaggtggc
     1501 gaactcgtca tcgttcgggt tgatgtcctc ggtctttacg gccgactacc ggcgggccct
     1561 gatcgtggcg gagcggctcg agtcgggcgg ggtcgtgatc aacggcaccg acaactaccg
     1621 cccgccggag ttccccttcg gcggcgtcaa gctctcgggc cggggtcgcg aggggatcgg
     1681 gtacacgctc gaggagctga cccgcgagaa gccgatcgtc ttccgcggtt tccgggaacg
     1741 gagttagcgg cgcgatgggc gccaaaccgg acggcaagta ccaggtcccg gccgtggtcg
     1801 gggcgatcag cgtgctgaac gagctcgccg ggaccgggga ggccggggca acccagtcag
     1861 agctggtcga ggccaccggc ctctccaaga gcaccatgca caacctgctc tcgacccttg
     1921 actcccacgg attcgtccgg cgcgacaacg gttcccgcca gtaccggctg ggaccggcgc
     1981 tgatcccgct cggcaacgaa gcgacccgcc aggtgaagct ggtccggacc acgcttgacc
     2041 gggtcgcgcc cctggccgcc gagtacgggc tctccttcgc ggtcgcccag ccgatcgatc
     2101 cggatgaact gcggatcatc agccgcttct atccccccgg ggtccacgtc gggatcacgg
     2161 tcggcagcgc ctacgggccg atggacgggg cgctgggcaa gatcctcctc tcctcgaagg
     2221 aggaggccgt ggccagaaag ctgatcaagg gccgcaaact gccggctcac acccgggcca
     2281 cgatcaccag tcccgaatcg ctgatcgagg agatcgccga agtccgaacc aggggctggt
     2341 cggtttccca cggcgagttc aacgagaaca acgccgtcgc ggccgggatc tggggt
//
LOCUS       DAOQKN010000274         2394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_33227_length_2394_cov_4.846943, whole genome shotgun sequence.
ACCESSION   DAOQKN010000274 DAOQKN010000000
VERSION     DAOQKN010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33227_length_2394_cov_4.846943"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1072
                     /locus_tag="PLJ64_12330"
     CDS             <1..1072
                     /locus_tag="PLJ64_12330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HNC94315.1"
                     /translation="DSLGPSTITRWAEFMNLFTGNGRIPVVPGLVLSLGGQLYEFLAD
                     AGSLPIVQSRFAEMENTPANVAAAKAEFRQDPRIRILMDNGAAVDGDPGAIGAKWEAD
                     FNSWPIRTTRPTAFYLGKNGTLSTSKSKTGSSVSYLSDPSARPAKTLGENAQGDSWLA
                     QPPYNWQPVADGHGLGFITPALTKDVFMAGPSSVDLYLKSSKPNTDIQVTLTEVRPDG
                     NETYIQNGWLRASHRAINSKLSTAYNPVQTWLKSDQAPLKKGKYNYARIQIFPAAHVF
                     RAGSKIRLNIQAPGGDRTIWDFDTIEKGNTTNTIGLGGVIPSKLVLPVIPGQDAQGTA
                     LPPATALRGEPSRDYVPASNGG"
     gene            1148..1498
                     /locus_tag="PLJ64_12335"
     CDS             1148..1498
                     /locus_tag="PLJ64_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94316.1"
                     /translation="MAKKRKPKRREREKSSTSSYADAEGNVLVLRDSLGKGSIEKINE
                     QIENQAYSVDDLWQRKTELIFERLAVSWEIAGLPLDDQKMLLGRYRMADPDTRRWVRE
                     ILDQHLRRYIPELA"
     gene            complement(1502..2296)
                     /locus_tag="PLJ64_12340"
     CDS             complement(1502..2296)
                     /locus_tag="PLJ64_12340"
                     /inference="COORDINATES: protein motif:HMM:NF011648.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC94317.1"
                     /translation="MNPLPPVEVEPHPWRGLAWLTRRECHRVLKLWKQTILAPVISSL
                     LFMIVFGLSLGDSIAEIGGFEYRVFIVPGLIAMAMAQSAYSNNSSTIFQARNDRYLDD
                     ILSAPMSAWQVHLGYLAGGAMRSLIIGVVLTALALPLTGAPLEMPLELIVAVVLTIGG
                     FGALGIIVGVYAQSWDNQSFINNIVILPLIFLGGVFYSVSRLGTPWEQISHLNPLFYV
                     VEAVRYGFLGAADVSPWLSLGVVGAITFALLAWAQFLFSTGHRLKP"
BASE COUNT          473 a          800 c          786 g          335 t
ORIGIN      
        1 cgattcgctc ggcccgagca cgatcacccg ctgggccgag ttcatgaacc tgttcaccgg
       61 taacggccgg attccggtcg tgcccgggct ggttctgagc ctcggcggcc agctctacga
      121 gttcctggcc gacgccggct cgctgccgat cgtccagagc cgcttcgcgg agatggaaaa
      181 caccccggcc aacgtcgcgg ccgccaaggc cgagttccgg caggatccgc gaatccggat
      241 cctgatggac aacggcgccg cggttgacgg tgacccgggc gcgatcggcg ccaagtggga
      301 ggccgacttc aacagctggc cgatcaggac gaccaggccc accgcgttct acctgggcaa
      361 gaacggcacc ctgagcacca gcaagtcaaa gaccggttct tcggtcagct acctgtccga
      421 cccgagcgcc cgtccggcca agaccctcgg cgagaacgcc cagggtgact cctggctggc
      481 ccagccgccg tacaactggc agccggtcgc cgacggccac gggctcggct tcatcacgcc
      541 ggccctgacc aaggacgtct tcatggccgg accgtccagc gttgatctct acctcaagtc
      601 gagcaagccc aacacggaca tccaggtgac cctgaccgag gtccgcccgg acggcaacga
      661 gacctacatc cagaacggct ggctgcgggc ctcgcaccgg gccatcaaca gcaagctctc
      721 aaccgcctat aacccggtgc agacctggct gaagtccgat caggctccgc tgaagaaagg
      781 caagtacaac tacgcccgga ttcagatctt cccggccgcc cacgtgttcc gggccggctc
      841 gaagatccgc ctgaacatcc aggcacccgg tggggaccgg acgatctggg acttcgacac
      901 gatcgaaaag ggcaacacca ccaacacgat cggtctcggc ggtgtgattc cgtcgaagct
      961 ggtcctgccg gtgatcccgg gccaggacgc ccagggcacc gcgctgcccc cggccaccgc
     1021 gctgcgcggc gagccgagcc gggattacgt gccggcgagc aacggaggct agaacataag
     1081 ggcgccgccc ggtcttcgcc gggcggtaac aggaacccgc ccggccaggg cctgacacac
     1141 tctgcgtatg gcgaagaagc ggaagccaaa gcggcgggaa cgggagaagt cctcgacctc
     1201 cagttacgcc gacgccgagg gcaacgtcct cgtgctgcgc gactcgctcg gcaagggatc
     1261 gatcgagaag atcaacgagc agatcgaaaa ccaggcttac tcggtcgatg acctctggca
     1321 gcgcaaaacc gaactgatct tcgaacggct ggccgtctcc tgggagatcg ccgggcttcc
     1381 gctcgacgac cagaagatgt tgctcggccg ctaccggatg gccgacccgg acacccgccg
     1441 ctgggtccgg gagatcctcg atcagcacct ccgccggtac attcccgaac tcgcctgaaa
     1501 gtcagggctt gaggcggtgt ccggtactga agaggaactg ggcccaggcg agcagggcga
     1561 aggtgatcgc cccgaccaca ccgagcgata gccaggggga gacgtcggcc gccccgagga
     1621 agccgtagcg gaccgcctcg accacgtaaa agagcgggtt gaggtgggag atctgctccc
     1681 agggggtgcc cagccggctg accgaataga agacgccgcc gaggaagatc agcggcagga
     1741 tgacgatgtt gttgatgaag gactggttgt cccaggactg ggcgtaaacg ccgacgatga
     1801 tgcccagcgc cccgaagccg ccgatcgtca ggaccaccgc gacgatcagc tccagcggca
     1861 tctccagcgg ggcgccggtc aggggcaggg ccagcgcggt gaggacgacc ccgatgatca
     1921 gcgagcgcat cgcgccaccg gccagatagc cgaggtgaac ctgccaggcg ctcatcgggg
     1981 cggagaggat gtcatccagg taccggtcgt tgcgggcctg gaagatcgtg ctcgagttgt
     2041 tcgagtaggc cgactgggcc atcgccatcg cgatcaggcc gggcacgatg aagacccggt
     2101 attcgaagcc gccgatctcg gcgatgctgt caccgagcga gagcccgaag acgatcatga
     2161 agagcaggga ggagatcacc ggggcgagga tggtctgctt ccagagcttc agcacccggt
     2221 gacattcgcg tcgggtcagc caggcgagcc cccgccacgg gtgcggctcg acttctacgg
     2281 ggggaagcgg attcaagctt ccccggcttc ttccctgacg atctcgatca cggccgagtc
     2341 gtcgagctcg ccgcggccac tctcgaccag ccggtcgagc gcctcgcggg cgac
//
LOCUS       DAOQKN010000275         2391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_33304_length_2391_cov_4.730308, whole genome shotgun sequence.
ACCESSION   DAOQKN010000275 DAOQKN010000000
VERSION     DAOQKN010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33304_length_2391_cov_4.730308"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /gene="tsaE"
                     /locus_tag="PLJ64_12345"
     CDS             <1..452
                     /gene="tsaE"
                     /locus_tag="PLJ64_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNC94318.1"
                     /translation="NFASGAGFVARGQEETEAAGRDLAALVQPGDLILISGEVGAGKS
                     TLVRAALRQLGVAGAIPSPTFTIGRLYEQPSQRMPVSHLDLYRLDGLGDEDPGLLAGY
                     LGPDRAAFVEWPGGSAGELSEMATRVGRVAIDHIDSESRRIRIDPPA"
     gene            complement(497..802)
                     /locus_tag="PLJ64_12350"
     CDS             complement(497..802)
                     /locus_tag="PLJ64_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94319.1"
                     /translation="MAILAVAGLGVIATGCGDSSSDSVQDTIDKANETANKALDQANK
                     QLDQANKQLDQANQNAQDQLDQAQQKLDNQGAQQKVDDAQKQLDDLQKQVNDAMNGQ"
     gene            908..1603
                     /gene="tsaB"
                     /locus_tag="PLJ64_12355"
     CDS             908..1603
                     /gene="tsaB"
                     /locus_tag="PLJ64_12355"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNC94320.1"
                     /translation="MIGFDTATDFTVVGATDAGRPIFERSLPPARRPLHSQALLENVT
                     GAVESLGGWDAVERIAVGIGPGTFTGIRIGVATAAGLAASTGIPVTGVPTLAALARSI
                     GEATGAGTVLPLLDARRGEVFGGLHDGRGEPLEEPFACGPEELTERLSGPGRSGPAPM
                     VGGSGAIRFRDHLVQAGLSLEPDESAVHRLTGRSICELGAAAETTGPENLQPLYLRVP
                     DAQLWLERDGKKV"
     gene            1578..2102
                     /gene="rimI"
                     /locus_tag="PLJ64_12360"
     CDS             1578..2102
                     /gene="rimI"
                     /locus_tag="PLJ64_12360"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HNC94321.1"
                     /translation="MNETARKSDSRNSGLSVRELAYADLPLIISIERRSFRSPWSVGM
                     FALEMSKVDTIGLAAVENDRVVGYLVLSRFDRAWHLMNVAVAPEHRRRGIASLLITDA
                     LKQIEEDEPVTLEVRPTNRSAIELYVGLGFRSYGLRRGYYPDNGEDALIMWKGDPEAA
                     GVPRESIADLPTGA"
     gene            2104..>2391
                     /locus_tag="PLJ64_12365"
     CDS             2104..>2391
                     /locus_tag="PLJ64_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNC94322.1"
                     /translation="MPLLAIETSCDDTCAAVLDGSRILSNVISSQADHERYGGVVPEV
                     ASRHHLELAVPVVEAALADAGVGFDDLDSVAVTAGPGLIGALLVGLSTAKAI"
BASE COUNT          362 a          770 c          819 g          440 t
ORIGIN      
        1 cgaatttcgc ttccggtgca ggctttgtcg cccggggaca ggaggaaacc gaggccgcgg
       61 gccgcgacct cgcggcgctg gttcaaccgg gggatctgat cctgatcagc ggcgaggtgg
      121 gggcgggaaa gtcaaccctg gtccgggcag ccctgaggca gctcggggtc gccggggcga
      181 ttccctcacc gaccttcacg atcggccggc tctacgagca gccctcgcag cgaatgcccg
      241 tttcccacct cgacctgtac cggctggacg ggctgggaga tgaggatccg ggtcttctgg
      301 ccgggtatct ggggccggac cgggcggcct tcgtcgagtg gcccggcggt tcggccggag
      361 aactaagtga aatggccacc cgggtcggcc gggtggccat tgatcacatc gattcggaaa
      421 gccgccggat caggatcgac ccgccggcct gaacggcctt actggccgca actcccgggt
      481 gtaaagatga acggccctac tggccgttca tcgcgtcatt gacctgcttc tggaggtcgt
      541 cgagctgctt ctgggcgtca tcgaccttct gctgcgcgcc ctggttatcc agcttctgct
      601 gggcctggtc gagctggtcc tgggcgttct ggttcgcctg atcaagctgc ttgttggcct
      661 ggtcgagctg cttgtttgcc tgatcgagtg ccttgttcgc ggtctcgttc gccttgtcga
      721 tcgtgtcctg gaccgaatcc gacgaggaat cgccgcagcc ggtggcgatg acgccaaggc
      781 cagctactgc gagaatcgcc aaggacgtgc cgagtttcct gcttccgatc atcaattcgt
      841 ctccagtcgt tatttctgcg gtggactttc tgctggtttc gccagcctat aggggtccgc
      901 ggtgaaaatg atcggcttcg acaccgcgac cgatttcacg gtggtcggcg ccaccgacgc
      961 cggacggccg atcttcgagc ggagcctgcc cccggccagg cgcccgctcc acagccaggc
     1021 cctgctcgag aacgtcaccg gggcggttga aagcctgggt ggctgggatg cggtcgagcg
     1081 gatcgcggtc ggaatcggtc ccggcacctt caccggcatc cggatcggtg tcgccaccgc
     1141 ggccgggctg gccgcatcga ccggcatccc ggtcaccggc gtcccgaccc ttgccgcttt
     1201 ggcccgttca atcggagagg cgaccggagc cggaaccgtg ttgccgctgc tcgacgcgcg
     1261 ccgcggagag gtcttcggcg gactccacga cggccggggc gagccgcttg aggaaccgtt
     1321 tgcctgcggg ccggaagagc tgaccgaacg gctgagcggg ccgggccgga gcggaccggc
     1381 accgatggtc gggggatccg gggcgatacg attccgcgac catttggttc aggccgggtt
     1441 gtctttagag ccggacgaat cagcggtcca ccggctgacc gggcgctcca tctgtgaact
     1501 gggagcggcc gccgaaacaa ccggaccgga aaatctccaa cccctctacc tgcgagtacc
     1561 cgatgcccaa ctctggcttg aacgagacgg caagaaagtc tgactcccgg aattccgggc
     1621 tcagcgtccg cgagctggcc tacgcggact tgccgctgat catctcgatc gagcgtcgct
     1681 cgttccgcag cccctggtcg gttggcatgt tcgcgctgga gatgtccaag gtggacacga
     1741 tcgggctcgc agcggtggag aacgaccggg tggtcggcta cctcgtgctc tcgcgcttcg
     1801 accgggcctg gcacctgatg aacgtggcgg tcgcgcccga gcaccggcgt cgcgggatcg
     1861 cttcgcttct gatcactgat gccctgaagc agatcgagga agacgagccg gtgacgctgg
     1921 aagtcaggcc gaccaaccgt tcggcgatcg agctttacgt cggcctcggg ttccgttcct
     1981 acggcctgcg ccggggctat tacccggaca acggtgaaga cgccctgatc atgtggaagg
     2041 gtgacccgga ggccgccgga gttccgcggg aatcgatcgc ggacctgccc accggggcct
     2101 gaaatgccgc tgctggcgat cgagacttcc tgcgacgaca cctgcgccgc ggtgctcgac
     2161 ggttcgcgga tcctctcgaa cgtcatttcc tcgcaggccg atcacgagcg ctacggggga
     2221 gtggtccccg aggttgcgtc acggcatcac ctcgagctcg ctgtgccagt ggtcgaagcc
     2281 gcgctggccg atgcgggggt cgggttcgac gacctcgact cggtcgcggt caccgccggg
     2341 cccgggctga tcggggcctt gctggtcggt ctcagtaccg ccaaggcgat c
//
LOCUS       DAOQKN010000276         2347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_34428_length_2347_cov_3.374782, whole genome shotgun sequence.
ACCESSION   DAOQKN010000276 DAOQKN010000000
VERSION     DAOQKN010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34428_length_2347_cov_3.374782"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="PLJ64_12370"
     CDS             <1..230
                     /locus_tag="PLJ64_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94323.1"
                     /translation="KLAGVPGAVIQAARRKLVMLENQQIAASPQGDLFAPAPSPEPAI
                     NPALERLLEIDPDELTPKEALALLYELRGLV"
     gene            complement(503..871)
                     /locus_tag="PLJ64_12375"
     CDS             complement(503..871)
                     /locus_tag="PLJ64_12375"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNC94324.1"
                     /translation="MPRSKPAETIFGKRLRESRLRAGIAQDKLGVMIGLDEGSSSARI
                     SRYETGIHEPTVSLAEKMADILHIPLAYFYCPDDELAGFIRDIGTLDRKGRMRLRAWL
                     DEVVGGGTSDCVPIDPAKWS"
     gene            987..1610
                     /locus_tag="PLJ64_12380"
     CDS             987..1610
                     /locus_tag="PLJ64_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94325.1"
                     /translation="MKYGSRIGAAALVSALIFGLQGCAAPIIAVGAATLGVAATQLYN
                     LARDQYPDINFEAPAPVEVTYAGSYESVWNALVDTLGEMHEQMAVVDKQSGLIRTVKN
                     NLNEESWIGKGLGKSTFKYEFNLTVRKNEKGIGVRSLVPFWEEKVFIAEKQKNLPEGT
                     NMMRHILYRNLAQKAKPLVAKMPDKPDTDVRYAPSENQAAPSAQKRP"
     gene            1636..2001
                     /locus_tag="PLJ64_12385"
     CDS             1636..2001
                     /locus_tag="PLJ64_12385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94326.1"
                     /translation="MQKGLGIAALVLAIIAIFIPFLGTWLTVVAAGLAAFAYGPGFAL
                     GVAGIIIDILHIFFFSPLLWATGGLAAVADGAAKAQGASGAEGASAFMALPWILVAIQ
                     IAALVFLIKTNKKQPTAAE"
     gene            2038..>2347
                     /locus_tag="PLJ64_12390"
     CDS             2038..>2347
                     /locus_tag="PLJ64_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94327.1"
                     /translation="MNILNTALATLFAAIFALPAQADTVQMIDNFESGILDLSKWDID
                     LGDTAHADVFNAISAFYENGEGPKLYYPSEGNWMLVASAGDPVTKLSTHFTTFPGGTF
                     G"
BASE COUNT          459 a          748 c          717 g          423 t
ORIGIN      
        1 ccaagctcgc cggcgtcccc ggcgccgtca tccaggccgc ccgccgcaaa ctggtcatgc
       61 tggaaaacca gcagatcgcc gccagccccc agggcgatct gttcgccccc gcgccgtcgc
      121 cggaaccggc catcaacccg gcgctggaaa ggctgctgga gatcgatccg gacgagctga
      181 cgccgaagga ggcgttggcg ttgctgtatg agttgagggg gttggtgtga gcgcaggcga
      241 ctcaaaccag cccgagggaa gcgcgcgagc gccatcccac aatagcgctc gccatcgacg
      301 agggtgaact tccgccacgc gccacctggc tggagggcac ggtcgccgtc ggttgatcga
      361 agcgtgaact actcctgatc cggcccgaat ccgtttgaac tcgccacccc ggcgtaggcc
      421 ggtgcccggt tgtggtgcca gcggaagaac tggataccgg ctttccccca ttcggggaca
      481 agcgccggta tgagggccac tgctaactcc acttggccgg gtcgatagga acacagtctg
      541 aagtcccgcc tcccaccacc tcatccagcc acgcgcgcag gcgcatcctc cccttcctgt
      601 ccaatgtccc gatatcgcgg atgaacccgg caagctcgtc gtctgggcaa tagaagtacg
      661 cgagggggat atggaggatg tcggccatct tctccgcaag cgagacggtc ggctcgtgga
      721 taccggtctc gtagcggctg atccgggcgc tgctcgaccc ctcgtcgagg ccgatcatga
      781 ccccgagctt gtcctgggcg atcccggccc ggaggcggct ctcccggagt cgcttcccga
      841 atatggtctc agcaggcttg cttctcggca tcctgcgata ttcgtaaatc gcgcttgacc
      901 cgactctacg gaatacatag atttatgacc catgaagtcc gtaggggcat atctgcggat
      961 acggggatgg acgaagggag agaagcgtga aatacggaag caggatcggg gcggcggcgc
     1021 tcgtttcagc acttatcttc ggactccagg ggtgcgcggc acccatcatc gcggtcggcg
     1081 ccgccacgct cggcgtcgcg gccacccagc tttacaacct cgctcgggac cagtatccgg
     1141 acatcaattt cgaggcgccc gctcccgtcg aggtcaccta tgccggaagc tatgaatccg
     1201 tctggaacgc gctcgtcgat accttaggcg agatgcacga gcagatggcc gtggtggaca
     1261 aacagagcgg tctcatccgg acggtgaaga acaacctcaa cgaggagagc tggatcggca
     1321 aggggctcgg gaagtcgacc ttcaagtacg agttcaacct caccgtccgg aagaacgaga
     1381 agggcatcgg cgtccggtcg ctcgtcccct tctgggagga gaaggtcttc atcgcggaga
     1441 agcagaagaa cctgcccgag gggacgaaca tgatgcggca catcctgtac cgcaacctcg
     1501 cccagaaggc gaaacccctc gtcgccaaga tgcccgacaa acccgatacg gacgtgcggt
     1561 acgccccgtc ggagaaccag gccgcgcctt cggcgcagaa acgtccgtaa cttcaaactt
     1621 caggagggaa tcaccatgca aaagggtctc ggtatcgccg cgcttgttct cgccatcatc
     1681 gccatcttca tccccttcct cgggacctgg ctcaccgtgg tcgcggcggg gctcgccgcc
     1741 ttcgcgtacg ggcctggatt cgcgctcggg gtcgcgggga tcattatcga catcctccac
     1801 atcttctttt tctccccgct cctctgggcg acgggtgggc tcgcggcggt ggcggacggt
     1861 gccgccaagg cccagggtgc ctcgggcgcc gagggggcga gtgccttcat ggctctccca
     1921 tggattctcg tcgcgatcca gatcgcggcc ctcgtctttc tcatcaagac caacaagaag
     1981 caaccgactg cggcggagtg acgctcatcg gaccgatacg acgggaagga actgaacatg
     2041 aacattctga atacagccct cgcgaccctt tttgccgcga tattcgccct gccggcacag
     2101 gcggacacgg tccagatgat cgacaacttc gaatcgggaa tcctggacct ctcgaagtgg
     2161 gatatcgatc ttggtgatac ggcgcacgcg gatgtcttca atgccatctc ggcgttttac
     2221 gaaaatggcg aaggtccgaa gctctattac ccgtccgagg gaaactggat gcttgtagcg
     2281 agcgccggtg acccggtaac caagctctcg acccatttca cgacttttcc gggtggcacg
     2341 ttcggga
//
LOCUS       DAOQKN010000277         2340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_34595_length_2340_cov_4.596937, whole genome shotgun sequence.
ACCESSION   DAOQKN010000277 DAOQKN010000000
VERSION     DAOQKN010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34595_length_2340_cov_4.596937"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="PLJ64_12395"
     CDS             <1..734
                     /locus_tag="PLJ64_12395"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNC94328.1"
                     /translation="LVGTTLQVLAEQSRFQFRLRKWQRDLKDHVVVCGFGIKGKSALE
                     YIQSHDEGIDAVVIDENDEALEDANRLDLNGINGKSYDRQVLDAARIGTAKFVIVALS
                     SDEQSILTVLRVRELNPSAKIVTSCKEESNVELLKSSGADEVIVSSSSAGRILGMAAE
                     TPEAARVVNDLLTFGDGLDINERHIETDGEQIEQRPHQTAIAVIRDQQVHRPGDPECR
                     TLKAGDQVIYIDQTAQEDSGQPTEA"
     gene            complement(913..1368)
                     /locus_tag="PLJ64_12400"
     CDS             complement(913..1368)
                     /locus_tag="PLJ64_12400"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002525071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNC94329.1"
                     /translation="MSKATLNTNAGPITIEFFDEDAPKTVENFRKLAADGFYDGLTFH
                     RVIKDFMIQGGCPLGTGTGGPGYTFEDEFNDHGVVRGALAMANAGPNTNGSQFFIVTI
                     PSADWLNGKHTVFGEVTDGMEVVDAIEGTQTDGRDMPLDPQVIESVELA"
     gene            complement(1414..1743)
                     /locus_tag="PLJ64_12405"
     CDS             complement(1414..1743)
                     /locus_tag="PLJ64_12405"
                     /inference="COORDINATES: protein motif:HMM:NF018021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HNC94330.1"
                     /translation="MEARITDEEFEELIIRAIDRLPEQFREIINQIPVVISDQGRERH
                     AYGLYQGDGIARDNYPDRIIIFKDTLVRDFGHDPALLAAQVERTVRHELGHHLGYDEG
                     GVRGLGL"
     gene            complement(1855..2151)
                     /locus_tag="PLJ64_12410"
     CDS             complement(1855..2151)
                     /locus_tag="PLJ64_12410"
                     /inference="COORDINATES: protein motif:HMM:NF021378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HNC94331.1"
                     /translation="MPGLIRGVARTAAIAGTATAVSNRVSRRQAAKWAEQDGPQYANP
                     RADMAQQQYQEPAPAPAAAPADPIAQLKELGELHQSGVLTDEEFAAQKAKILGS"
BASE COUNT          388 a          763 c          765 g          424 t
ORIGIN      
        1 tgctggttgg cactacgctt caggtcttgg ccgagcagtc cagattccag tttcgtcttc
       61 gcaagtggca gcgtgacctg aaggatcacg tggtcgtctg cggattcggg atcaagggca
      121 agtccgccct cgagtacatc cagagccacg acgaaggcat cgacgccgtc gtgatcgacg
      181 agaacgacga ggccctcgaa gacgcgaacc gcctcgacct gaacgggatc aacggcaagt
      241 cctatgaccg gcaggttctc gacgcggcgc ggatcggcac ggcgaagttc gtgatcgtcg
      301 ccctcagctc cgacgaacag tcgattctca cggtcctccg ggtccgtgag ctgaacccgt
      361 ccgcgaagat cgtcacctcc tgcaaggagg aatcgaacgt cgagctgctc aagtcatcgg
      421 gcgccgacga ggtgatcgtt tcctccagct cggccggccg catccttggc atggcggccg
      481 aaaccccgga agccgcccgg gtggtcaacg acctgctcac tttcggagac ggcctcgaca
      541 tcaacgaacg ccacatcgaa accgacggcg agcagatcga gcagcgtccc catcagaccg
      601 cgatcgccgt gatccgcgac cagcaggttc accgtcccgg cgaccctgag tgccgcaccc
      661 tcaaggccgg cgaccaggtc atctacatag accagaccgc ccaggaagat tcagggcaac
      721 ccaccgaagc ctgaccctcc cgccccgggt ggtcgcgccg ggcgagcccg ccgaaaagca
      781 ccgcgagtgc ccactttgtg ccacataccg gcacaaacag ggcactcgac gagtccgaac
      841 ccgccccggc gccggggcgg gtcggattcc cgccccgttg cgggtcggat tcccggcccc
      901 gatgcggccg atctaagcca gctcgactga ctcgattacc tggggatcca ggggcatgtc
      961 gcggccgtcg gtctgggtgc cttcaatcgc gtcgaccact tccatgccgt cggtgacctc
     1021 accgaagacg gtgtgcttgc cgttcagcca gtcagccgag gggatggtga cgatgaagaa
     1081 ctgcgagccg ttggtgttgg ggccggcgtt cgccatcgcg agggcgccgc ggaccacgcc
     1141 gtggtcgttg aactcgtcct cgaaggtgta gccggggcca ccggttccgg tgccgagcgg
     1201 gcagccgccc tggatcatga agtccttgat cacgcggtgg aaggtgaggc cgtcgtagaa
     1261 accgtcggcg gcgagcttgc ggaagttctc gaccgtcttc ggcgcgtcct catcgaagaa
     1321 ctcgatggtg atcggaccgg cattcgtatt cagcgttgct ttcgacatgg gtcgagccta
     1381 caggccgatg gatctccggc tcgccgatcc gggtcagagc ccgaggcccc gcacaccacc
     1441 ctcgtcgtac ccgaggtggt ggccgagttc atgccgcacg gtgcgttcga cctgggcggc
     1501 gagcagggcc gggtcgtggc cgaagtcacg gaccagggtg tccttgaaga tgattatccg
     1561 gtccgggtaa ttgtccctgg cgatgccatc accctggtag agaccgtagg catgccgttc
     1621 ccggccctgg tcggagatca ccaccggaat ctggttgatg atctcccgga actgctcggg
     1681 cagccggtcg atcgcccgga tgatcagttc ctcgaactct tcgtcagtga tccgggcctc
     1741 catgcccgaa tcattgcgaa gtcgactact ggctgagtcc gaccatccat ttcttgatcc
     1801 cgtgatcgga cggttcctgt tgcgatccag cgaagaccgg atcgcgcgtt tgtgtcagct
     1861 gccgaggatc tttgccttct gggcggcgaa ttcctcgtcg gtcagaaccc cgctctggtg
     1921 aagctcgccg agttccttga gctgggcgat cgggtcggcc ggggcggcgg cgggcgccgg
     1981 ggcgggctcc tggtactgct gctgggccat gtcggcccgg gggttcgcgt actgcggccc
     2041 gtcctgctcg gcccacttgg cggcctggcg acgggaaacc cggttgctta cggcggtggc
     2101 ggtaccggcg atcgccgcgg tgcgggcgac gcctctgatc agtcctggca tttcggttct
     2161 ccttgcttct gtgaggtttc taggcttcgg ccgagtcgag gctggcgatc agggcctgga
     2221 ccgggatccg gccgctggca accagctgcc cgccggaacg gcgtacggcg gaagcaaacg
     2281 gtccggccca gctgttctcg tagaccagca acgccgcggc cttgccggtc tccagggctt
//
LOCUS       DAOQKN010000278         2333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_34786_length_2333_cov_4.150132, whole genome shotgun sequence.
ACCESSION   DAOQKN010000278 DAOQKN010000000
VERSION     DAOQKN010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34786_length_2333_cov_4.150132"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            226..1968
                     /locus_tag="PLJ64_12415"
     CDS             226..1968
                     /locus_tag="PLJ64_12415"
                     /inference="COORDINATES: protein motif:HMM:NF023500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3556 domain-containing protein"
                     /protein_id="HNC94332.1"
                     /translation="MSPSDPPFDSIEEWRKLPYRERIKPLAQDWAVNGIGVPQAVYLL
                     YIVKLIVYAGGGLLLISATSDLGGLSEISDWWTQPVVFQKAVVWTMLWEALGLGAGSL
                     PLTLRFSPMVGGFLHWLRPGTIRQPAWPDKVPLTAGDSRTILDVFLYAGLICSAIVLL
                     VGSGADGFDPVIVGVLLAFLVLLGLRDKVPFLAARAEVYGPFLLIFCFPADQMIVAAQ
                     FVMFCIWWGAASSKLNRHFPFVVTVMISNTPWNRSKRAKRKLYRNHPEDLIPSRIGEL
                     SAHMGTVLEFTLPLLLIASSGGVIGTIAVAGMVIFHLHILSTFPLAVPLEWNIFMIFS
                     IFFLFGHYGDVPLSTLDDPLLIACLLPLIVGIPVLGNFRPDLVSFLPSMRYYAGNWAT
                     SQWYFLKGSGAEAKLDGPITKAAKTLPVQLGKFYEPDMNEVLLHKGLAFRSMHTHGRA
                     LVGLSERAVDDVENYDVREGETISGMICGYNFGDGHFHNEKLLASVQSRVGYEPGELR
                     VVTLESQPAGNPFQRYRIFDAAEGLIEEGTVKVTDMVVRQPWLGVDEVFPVTVERGGR
                     EASPVAAGAEEPGR"
     gene            1992..>2333
                     /locus_tag="PLJ64_12420"
     CDS             1992..>2333
                     /locus_tag="PLJ64_12420"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC94333.1"
                     /translation="MPQVHIVGSGPNGLTCAAVLARAGVEVTVYEAGSTIGGGTRSVD
                     LTGQGLIHDVCSAVHPMAVASPAFLELDLEAHGLEWCFPEVDLAHPLDGGRAGVMVRD
                     IEMTAAGLGEDG"
BASE COUNT          336 a          778 c          789 g          430 t
ORIGIN      
        1 cggatgcatc ggaatctcct tggttcgggg gtaactgacg acccgcagac tgccggtcgg
       61 gccttgccgg gttcttgccg tcaccttgct ggggctccgg gcggtcgcga atgcccggtt
      121 tgtgccggta tgtggcgcca agcgggcatt cgcgaatgcg gggaatcgag cgataagctg
      181 cggggcgttg gaggtcgtcc tcaggagggg tgcttcatgg gtttcatgag tccgagtgat
      241 ccgccgtttg actcgatcga ggagtggcgg aagcttccct accgggaacg gatcaagccg
      301 ctggcccagg actgggcggt caacgggatc ggggtgccgc aggcggtcta cctgctctac
      361 atcgtcaagc tgatcgtcta cgccggcggc gggctgctcc tgatctccgc cacctccgac
      421 ctcggcgggc tctcggagat ctccgactgg tggacccaac cggtcgtctt ccagaaggcc
      481 gtggtctgga ccatgctctg ggaggcgctc ggcctcgggg ccggctcgct tcccctgacc
      541 ctgcgctttt ccccgatggt cggcggcttc ctccactggc tcaggccggg gacgatccgc
      601 cagccggcct ggccggacaa ggtgccgctg accgccggtg acagccgcac catcctcgac
      661 gtcttcctct acgccggtct gatctgcagc gcgatcgtgc tgctcgtggg cagcggcgcc
      721 gacggcttcg acccggtgat cgtcggcgtc ctgctcgcct tcctggtgct gctcggcctg
      781 cgggacaagg tgccgttcct cgccgcccgg gccgaggtct acggaccgtt cctcctgatc
      841 ttctgcttcc cggccgacca gatgatcgtc gccgcgcagt tcgtcatgtt ctgcatctgg
      901 tggggcgccg cctcctcgaa gctgaaccgg cacttcccct tcgtggtcac ggtgatgatt
      961 tccaacacgc cgtggaaccg cagcaaacgg gcgaaaagga agctctaccg caaccacccg
     1021 gaggacctga tcccctcccg gatcggcgag ctttcggccc acatggggac ggtcctcgag
     1081 ttcaccctgc cgctgcttct gatcgcctca tccggtgggg tgatcgggac gatcgccgtg
     1141 gccgggatgg tgatattcca ccttcacatc ctctcgacct tccccctggc ggtcccgctc
     1201 gagtggaaca tcttcatgat cttctcgatc ttcttcctct tcggccacta cggggacgtg
     1261 cctctttcga ccctcgacga tccgctcctg atcgcctgcc tgctgccgct gatcgtcggc
     1321 atccccgtgc tcgggaactt ccggccggac ctggtcagct tcctgccctc gatgcgctac
     1381 tacgcgggca actgggcgac cagccagtgg tacttcctga aaggttccgg ggcggaggcg
     1441 aagctcgacg ggccgatcac caaggccgcg aagaccctgc cggtccagct cgggaagttc
     1501 tacgaaccgg acatgaacga ggtgctgctc cacaaggggc tcgccttccg ctcgatgcac
     1561 acccacggcc gggcccttgt cgggttatcc gagcgggcgg tcgatgacgt cgagaactac
     1621 gacgtgcggg aaggcgagac gatctcgggg atgatctgcg gctacaactt cggggacggc
     1681 cacttccaca acgagaagct gctcgcctcg gtccagtctc gggtcggcta cgagccgggt
     1741 gagctcaggg tcgtgaccct ggagtcgcaa ccggccggca acccgttcca gcggtaccgg
     1801 atcttcgacg ccgccgaggg gctgatcgag gaagggaccg tgaaggtcac cgacatggtg
     1861 gtccggcagc cctggctggg ggtggacgag gtcttcccgg tgaccgtcga gcgcggaggc
     1921 cgcgaggcca gcccggttgc ggccggggcg gaggagcccg gccggtgaag ccgggccggt
     1981 agcttcgagc catgccgcaa gtccacatag tcggatccgg gccgaacgga ctgacctgcg
     2041 cagccgtgct ggcccgggcc ggggtggagg tcaccgtgta tgaggcgggg tcgacgatcg
     2101 gcggcggcac ccgttcggtc gacctgaccg gccagggcct gatccacgac gtctgctcgg
     2161 ctgtccaccc gatggccgtc gcctcgcctg ccttcctcga actggacctc gaagcccacg
     2221 gactcgagtg gtgcttcccg gaggtcgacc tcgcgcatcc gctcgacggg ggccgggccg
     2281 gggtgatggt ccgcgacatc gaaatgaccg cggctgggct gggggaggac ggc
//
LOCUS       DAOQKN010000279         2289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_36040_length_2289_cov_4.456132, whole genome shotgun sequence.
ACCESSION   DAOQKN010000279 DAOQKN010000000
VERSION     DAOQKN010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36040_length_2289_cov_4.456132"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..132
                     /locus_tag="PLJ64_12425"
     CDS             <1..132
                     /locus_tag="PLJ64_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNC94334.1"
                     /translation="AQILMRRLGVAATTRASFAAYNTTEEIDTLVEALHDARRIFGL"
     gene            178..765
                     /locus_tag="PLJ64_12430"
     CDS             178..765
                     /locus_tag="PLJ64_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94335.1"
                     /translation="MSQRRITPSLVISVIALIVALGGASYAALRIPRNSVGAGQLKRN
                     SVSASKLRKGSVNSSKVKDRSIRSRDLAPGVIPGATWRGGRDSSTLLDLTGTMQVVAS
                     TPVLPAGSYVVWSRANVLGGVGSSTIICSVASDAAQNITIPAGAVFPLSMAATKVLEE
                     PGPIELNCNKSSGSPQVAQAQVIATRVPKLTAPPE"
     gene            865..1914
                     /locus_tag="PLJ64_12435"
     CDS             865..1914
                     /locus_tag="PLJ64_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94336.1"
                     /translation="MEVKLGVPEGEADEFDTLPPADLPAHEKKLIDARNHLVQTSQAA
                     RIWAEENPAHPVSYLVTRSDVSEQTKIQALRRIESVSLPFQVNELLDAARHFNECQAV
                     SQAAFDAEQPVAEDDLPDHGDRDIFEGLRAAYLNLLRGYRNWGGFGYHGWTTKRDPDS
                     YPGPAIWTRQDCVFRFGRALEREFPDRVHLGLKISKSTTESFDPAVDRRQSVDITVTV
                     PGLFSRDLSARRFRNLRHDLFVEVEWLKKGRWLNTDQLVKRCGRIQQNLINLQRSIDS
                     GRCEFAAMLIVDDEGFLDFHGDLLRWPPDVIPLVVSPGEVVELGLDDGSVEPVLDEIE
                     SLHDSACPCSDGPLA"
     gene            1958..2251
                     /locus_tag="PLJ64_12440"
     CDS             1958..2251
                     /locus_tag="PLJ64_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94337.1"
                     /translation="MDPEDDLVIFPREDVEACLRNEKMQQEANSDGSPSGQSQAMETS
                     RLGAIEAGAPERIRWIYFESPEWTWQNLCGRSGWLLIDPETIDQYDFVMTMMN"
BASE COUNT          415 a          762 c          713 g          399 t
ORIGIN      
        1 gcccagatcc tgatgcgccg cctcggcgtc gctgccacca cccgcgccag cttcgccgcc
       61 tacaacacga cggaggaaat cgacaccctg gtcgaagccc tccatgacgc ccgccgcatc
      121 ttcggcctct gaacggttgg cgttgacagg accgtcccgg cggtgtaagc caagggcatg
      181 tctcaacgtc gaatcacgcc ctcgctcgtc atttccgtca tcgcgctgat tgtcgccctg
      241 ggtggtgcca gctacgcggc cctcaggatt ccgcgaaact cggtcggcgc cggtcaactg
      301 aaaaggaact ccgtttcggc gtccaagttg aggaaggggt cggtcaactc gtcgaaggtc
      361 aaagaccggt cgatccggtc ccgggatctg gcgccgggag tgattcccgg cgccacctgg
      421 cgaggaggcc gggactcctc gacgctgctt gatctgaccg ggacgatgca ggtggttgcg
      481 agcacgcccg tgcttccggc cggttcatat gtcgtctgga gccgggcgaa tgtccttggc
      541 ggcgtcggca gttcgacgat catctgctcg gtcgcttccg acgcggcgca aaacatcacc
      601 atcccggcgg gtgcggtctt cccgctctcg atggcagcga ccaaggtcct cgaagagccc
      661 ggcccgatcg agctcaactg caacaagtcc agcggttccc cgcaggtggc gcaggcccag
      721 gtcatcgcga cgcgggtccc gaaactcacc gcacccccgg aatgattccg ctccccgggg
      781 aacgtcaccg tccccgtgca ggactgcctt cagtctgacc cgtttcaccc cccgcaacag
      841 cagaccacat ctacgatttc gcgcatggaa gtcaagttgg gggtgccgga gggagaagcc
      901 gacgagttcg acacgcttcc acctgccgat ttgccggccc acgagaagaa actgattgac
      961 gcccggaacc acctggtcca gaccagccag gcggcccgga tctgggccga ggagaacccg
     1021 gcccaccctg tttcctatct cgtcacccgc agcgacgtca gtgaacaaac caagatccag
     1081 gccttgcgga ggatcgagtc ggtttcactg cccttccagg tgaacgaact ccttgacgcg
     1141 gcccgccact tcaacgaatg ccaggccgtt tcccaggccg cctttgatgc cgagcagcca
     1201 gtcgccgagg acgacctccc agaccatggt gaccgggata tcttcgaagg cctgcgtgcc
     1261 gcctatctca acctgcttcg cggctatcgc aactggggtg gcttcggcta ccacggctgg
     1321 accacgaagc gggacccgga cagctatccc ggcccggcga tctggacccg ccaggactgc
     1381 gtgttccggt tcggacgggc gctggagcgg gagtttccgg accgggttca cctcggcctg
     1441 aagatcagca agtcgacgac ggagagcttt gacccggcgg ttgaccgccg gcagtccgtg
     1501 gacatcacgg tcaccgtgcc cggattgttc tccagggacc tgagcgcacg ccggttccgg
     1561 aacctgcgcc acgatctctt cgtcgaggtt gagtggctga agaagggccg ctggctgaac
     1621 accgaccagc tggtcaagcg ctgcggccgg atccagcaga acctgatcaa cctgcagcgc
     1681 tcgatcgaca gcgggcgctg cgagttcgcc gcgatgctga tcgtcgacga cgaaggcttc
     1741 ctcgacttcc acggcgacct tctcaggtgg ccgccggacg tgattcccct ggtggtcagc
     1801 ccgggagagg tggttgagct cggcctcgac gacggctcgg tcgaaccggt cctcgacgaa
     1861 atcgaatccc tccacgactc cgcctgcccc tgctccgacg gccccctggc ctgaccggac
     1921 cctgtcaggt ccgggccggg ctcgcgactc tcaactcatg gacccggaag atgacctggt
     1981 gatctttccc cgggaagacg ttgaagcgtg tttgcggaac gagaagatgc agcaggaagc
     2041 caattcggac ggttctccgt ccgggcagag tcaggcgatg gaaacgagtc ggctcggcgc
     2101 gattgaggcc ggagcacccg agcgaatccg gtggatctac ttcgagtcac ccgaatggac
     2161 ctggcagaac ctttgcgggc gctccggctg gttgctcatc gatccggaaa cgattgacca
     2221 gtacgacttc gtgatgacaa tgatgaactg acccggcaag ggttcgtggc ctgggagcgg
     2281 cggtcagct
//
LOCUS       DAOQKN010000280         2284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_36178_length_2284_cov_5.505159, whole genome shotgun sequence.
ACCESSION   DAOQKN010000280 DAOQKN010000000
VERSION     DAOQKN010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36178_length_2284_cov_5.505159"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /gene="otsB"
                     /locus_tag="PLJ64_12445"
     CDS             complement(<1..721)
                     /gene="otsB"
                     /locus_tag="PLJ64_12445"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HNC94338.1"
                     /translation="MTGTDSPLSVLLEDPANSALMSDVDGTLCRIVDRPDQARVPVRA
                     RESLETLADHLALVACITGRPSPVARKMVGAKKITYFGNHGLSFIKPGQDRPTVVIEG
                     EEAERARNFIRENDNPFWVTSKIRIEDKGPIQALHWRGAGHRTEEVIRGIADHAQSAG
                     LATHWGRKVLELRPPGMEGKAGAVDELLDGTDIDTVVFAGDDRTDYDAAVRLKKLADE
                     GQLKRLLLVAIDSDEGPPELVE"
     gene            779..1144
                     /locus_tag="PLJ64_12450"
     CDS             779..1144
                     /locus_tag="PLJ64_12450"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNC94339.1"
                     /translation="MDKRRIIGLAGVGTLAGAFSGLFGVGGGTVIVPMLIALYHYGDR
                     LATGTSLAAIIVIAILAAAMHGGIYGNVDLETGLILVGPAIIGVVAGTAIQQRIPEKT
                     VSFLFALLLIGVAIEMVVG"
     gene            1144..1617
                     /locus_tag="PLJ64_12455"
     CDS             1144..1617
                     /locus_tag="PLJ64_12455"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNC94340.1"
                     /translation="MDIAIACAICLMGGIVAGLIGVGGGIVFVPAMTLVLSKGQVEAE
                     ATSLMMIALVSVVGAWRQYGYGNVNMRDAVVIGLLSPVGVLVGVVVANSVPERTLRIG
                     FAMLALYMAWRLLRRVFGNDPHDPAEPGTEPLADPGPDPPKGAGPEPLTGSGPAR"
     gene            1614..2030
                     /gene="arfB"
                     /locus_tag="PLJ64_12460"
     CDS             1614..2030
                     /gene="arfB"
                     /locus_tag="PLJ64_12460"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HNC94341.1"
                     /translation="MKPGIRPRGGPFIPVGEITLRASRSSGPGGQHANVTASRIEAVF
                     EVESSLALNDAEKARIIRKLGPTVTAVSQDARSQTRNRDLALERLETKLAGALSVPRR
                     RRATRPTRASKQKRLESKRHQSRKKQDRRRPGPDYD"
     gene            complement(2032..>2284)
                     /locus_tag="PLJ64_12465"
     CDS             complement(2032..>2284)
                     /locus_tag="PLJ64_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94342.1"
                     /translation="LLTAKTNVVNQYASGDNMIECTIWTDEVDGVDRATSDVPYNRIF
                     NLSAMAVADVQGLVELRCVAWNGPAQLFDTKLIAQPIQG"
BASE COUNT          352 a          771 c          764 g          397 t
ORIGIN      
        1 gttcgaccag ttccggcggg ccttcgtccg agtcgatcgc gaccagcagc aggcgcttca
       61 gctgaccctc atcggcaagt ttcttgaggc ggacggcggc gtcgtaatcg gtgcggtcgt
      121 caccggcgaa gaccaccgtg tcgatgtcgg ttccgtcgag cagctcgtcg accgcgccgg
      181 ccttgccttc catcccgggg ggacgaagct cgagtacctt ccggccccaa tgggtcgcaa
      241 gcccggccga ctgggcgtgg tcggcgatgc ccctgattac ttcctcggtc cggtggccgg
      301 cgccgcgcca gtggagggcc tggatcggac ccttgtcctc gatccggatc ttcgaggtca
      361 cccagaaggg gttgtcgttc tcgcggatga agttccgggc ccgctccgcc tcttcgccct
      421 cgatcaccac ggtcggccgg tcctggccgg gcttgatgaa gctgaggccg tgattgccga
      481 agtaggtgat cttcttggct ccgaccatct tccgggcgac cggcgagggg cgacccgtga
      541 tgcaggcgac cagcgcgagg tggtcggcca gggtttcgag gctttcccgg gcccggaccg
      601 gaacacgggc ctggtcgggt ctgtcgacga tccggcagag cgtgccgtcg acgtcactca
      661 tcaacgccga gttcgcgggg tcttccagaa gtaccgagag aggagagtct gtgcccgtca
      721 tatgcgagag actaccccgg tccgcctgcg ggtcgaaaaa gtctcctagt atcgcgccat
      781 ggacaagcgg cgcataatcg gcctggcggg agtgggcact ctggccggag cattcagcgg
      841 actcttcggg gtcggaggcg gcacggtgat cgtgccgatg ctgatcgcgc tctatcacta
      901 cggcgaccgt ctggcgaccg gaaccagcct cgccgcgatc atcgtaatcg cgatcctggc
      961 ggccgcgatg cacggcggga tctacggcaa cgtcgatctc gagaccggcc tgatcctggt
     1021 cggtcccgcg atcatcggag tggtcgccgg cacggcgatc cagcaacgca tccctgagaa
     1081 gacggtctcc tttcttttcg ccctgctgct gatcggagtg gcgatcgaga tggtggtcgg
     1141 ctgatggaca tcgcgatcgc ctgcgccatc tgcttgatgg ggggaatcgt cgccggactg
     1201 atcggggtcg gcgggggcat cgtcttcgtg ccggcgatga ccctggtcct ttccaagggc
     1261 caggttgaag cggaagcgac ctcattgatg atgatcgcgc tcgtttcggt ggtcggggcc
     1321 tggcggcagt acggctacgg caacgtcaac atgcgggacg ccgtggtcat cggcctgctc
     1381 tcgccggtcg gcgtgctggt cggggtggtc gtcgccaact cggtccccga gcggacgctc
     1441 cggatcggct tcgcgatgct cgccctttac atggcctggc ggctgctcag gcgggtcttc
     1501 ggcaacgacc cgcatgaccc ggccgaaccc ggcaccgagc cgctcgccga ccccggtccc
     1561 gacccgccga agggtgccgg cccggaaccg ctgaccggct ccggcccggc ccgatgaagc
     1621 ccggcatccg accccgcggc gggcccttca tcccggtcgg cgaaatcacc ctgcgagctt
     1681 cccggtcctc cggcccgggt ggccagcacg ccaatgtcac cgcctcgcgg atcgaggccg
     1741 tcttcgaggt cgagtcctca ctcgccctga acgatgccga gaaggcgagg atcatccgga
     1801 agctcggacc gacggtcacc gcggtcagcc aggacgcccg ctcccagacc cgcaaccgcg
     1861 acctcgccct cgagagactc gaaacgaagc tggccggcgc gctttcggtc ccgcgccggc
     1921 gccgtgcgac caggcccacc cgcgcctcga agcagaagcg cctcgagtcg aagcgccacc
     1981 agtcccgcaa gaagcaggac cggcgacgcc ccggacccga ttacgactga atcagccctg
     2041 gatcggctgg gcgatcagct tggtgtcgaa cagctgggcc ggaccgttcc aggcgacgca
     2101 gcgcagttcg accagaccct gcacatctgc cacggccatc gccgagaggt tgaagatcct
     2161 gttgtacggc acgtcgctcg tcgcccggtc gacaccgtcg acctcatcgg tccagatggt
     2221 gcactcgatc atgttgtcgc cggatgcgta ctggttcaca acgttggtct tggcggtgag
     2281 cagg
//
LOCUS       DAOQKN010000281         2263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_36765_length_2263_cov_4.247736, whole genome shotgun sequence.
ACCESSION   DAOQKN010000281 DAOQKN010000000
VERSION     DAOQKN010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36765_length_2263_cov_4.247736"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="PLJ64_12470"
     CDS             complement(<1..594)
                     /locus_tag="PLJ64_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94343.1"
                     /translation="MKTKALVPIALLLALALLPGAAGAADTYVAPNGSGNACTQASPC
                     SILTGGNEADADSAIILAPGDYGSAVDPIDDRIYTFAPNANYVGRNDVRLFVDASSST
                     ASVTIYSGQKFLGYGTRIISFDEVGVGIYGSARAERIDVRTSIDGSTACRFGNEGMET
                     GGQLTNSLCVADGFGSKGIELAATRNEESAMVMGSTGV"
     gene            complement(731..1978)
                     /locus_tag="PLJ64_12475"
     CDS             complement(731..1978)
                     /locus_tag="PLJ64_12475"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HNC94344.1"
                     /translation="MADIRPLHALHYDLRAIGSLGEVAAPPYDVISGPERSSMLDTSP
                     FNVVEIDLPESTGGDPYEHASGTLEEWILSGILKQDREPSIWALTQEFTGPDGSMKIR
                     NAILARVRVEDYGPGRIRPHERTQPGPKQDRLDLTRATRYNLSPIFSLTTRDAWPVVA
                     PAIEGEEAWAEVLGGDGTLNRVWKIEDPEIHRAVSQELDQAELLIADGHHRYETARAY
                     RDEVGGEGAHCYTLMALTGLDDPGLTVFPTHRLLTGLKDPDLVDRLKRGVEGAFESIA
                     EGEIDPSGVEGTGCFGLALSDGSRQLLRLRDPASLNALFPDESPAFRDLDAAILEKVV
                     LGGVLGMTETDVEAKRGLAYAKTIGEATAAVDSGEAQAAFILRATPIGQVRAVAEAGE
                     TMPPKSTYFFPKIPTGIVFNPLS"
     gene            2041..>2263
                     /locus_tag="PLJ64_12480"
     CDS             2041..>2263
                     /locus_tag="PLJ64_12480"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HNC94345.1"
                     /translation="MAPEGDSQEKLPWALVGAGPTGLSAARNLSRRGIPFVGFEIHTD
                     VGGLWDIESEKSTMYESAHLISSKTTTAYD"
BASE COUNT          373 a          703 c          772 g          415 t
ORIGIN      
        1 aactccggtc gaacccatga ccatcgctga ttcttcattc cgggtcgcgg ccagctcgat
       61 gcctttggac ccgaatccgt cggcaacgca aagcgagttg gtgagctggc ctccggtctc
      121 catcccttcg ttgccgaagc ggcaggcagt gctgccgtcg atgctcgtcc tgacatcgat
      181 tctttcggcg cgggccgaac cgtagatgcc gaccccgacc tcatcgaagc tgatgatcct
      241 ggtcccgtag ccgaggaact tctggccgga gtagatggtc accgaggcgg tgctggacga
      301 ggcatcgacg aacaaccgaa catcgtttcg gccgacatag ttggcgttcg gcgcgaatgt
      361 gtagatccgg tcgtcgatcg ggtctacggc ggatccgtaa tcgccaggag ccaggatgat
      421 cgccgagtcc gcgtcggctt cattcccgcc ggtgaggatc gagcagggcg acgcctgggt
      481 gcaggcgttg ccggagccgt tcggcgcaac ataggtgtcg gccgctccgg ccgctccggg
      541 gagcagggcg agggcgagga gcagggcaat cgggacgagc gctttggtct tcatcgagtt
      601 cagcatccgg ccgaagccgg gacctggcta cgacccaagg gctgaaaccg gccatcccgg
      661 ccgcttggct tcacctgtgt acggattaac ccggcatacg gggttaatcg atccacagcc
      721 gggaatccgg tcagctcagg gggttgaaaa cgatgccggt cgggatcttg gggaagaagt
      781 aggtcgactt cggtggcatg gtttctcccg cctcggcgac cgcccggacc tgcccgatcg
      841 gtgtggcacg gaggatgaag gcggcctggg cctctcccga atcgaccgcc gcggttgcct
      901 ccccgatcgt ctttgcgtag gcgagccccc gtttggcttc gacatcggtc tcggtcatcc
      961 cgaggacgcc gcccaggacg accttttcga ggatcgccgc atccagatcc cggaaggctg
     1021 gcgactcatc cgggaacagg gcattgaggg aggcgggatc ccggagccga agcagctgcc
     1081 tcgatccgtc cgaaagggca aggccgaagc atccggttcc ttcgactccg gacgggtcga
     1141 tttctccttc agcgatcgat tcgaacgccc cttcaacgcc tctcttgagg cgatcgacca
     1201 ggtcggggtc cttgaggccg gtcaggaggc gatgggtggg gaacacggtc aggcccgggt
     1261 catcgagtcc ggtcagggcc atcagggtgt agcagtgagc gccctcgcca ccgacctcat
     1321 cccggtaggc gcgggcggtc tcgtagcggt gatggccgtc ggcgatcagc agttcggcct
     1381 gatcaagttc ctggctgacc gcgcggtgga tctccgggtc ttcgatcttc cagacccggt
     1441 tcagggtgcc gtccccgccg agcacctcgg cccaggcttc ctcgccttcg atcgccgggg
     1501 cgaccaccgg ccaggcgtca cgggtggtca gcgagaagat cggcgagagg ttgtaccggg
     1561 tggcccgggt caggtcgagc cggtcctgct tcgggccggg ctgggtccgc tcgtggggcc
     1621 ggatccggcc gggcccgtaa tcctcgaccc gcacccgggc gaggatcgcg ttgcggatct
     1681 tcatcgaacc gtcgggcccg gtgaactcct gcgtcagggc ccagatcgag ggttcacgat
     1741 cctgtttgag gatgccgctg aggatccact cttcaagggt tccggatgcg tgttcgtagg
     1801 ggtcgccacc ggtcgactcc ggcaggtcga tctcgaccac gttgaacggt gaagtgtcga
     1861 gcattgagga ccgttccggg ccgctgatca cgtcatacgg cggcgccgcg acttcgccga
     1921 gcgacccgat cgcgcgaagg tcgtaatgga gggcgtgaag gggtcggatg tctgccatgg
     1981 gcaaacggta gcggtctcga ctgttggcgc ctggtcaccg gtgccatagg attcgcgatc
     2041 gtggcgcctg agggggattc acaagagaaa ctgccgtggg cattggtcgg ggccggcccg
     2101 accggactct cggcggcacg gaacctctcc cgccgcggta ttcccttcgt cggattcgag
     2161 atccacaccg atgtcggcgg gctctgggac atcgagtcgg agaagtcaac gatgtatgag
     2221 tcggcccacc tgatctcctc gaagacgacc accgcctacg acg
//
LOCUS       DAOQKN010000282         2246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_37289_length_2246_cov_3.785942, whole genome shotgun sequence.
ACCESSION   DAOQKN010000282 DAOQKN010000000
VERSION     DAOQKN010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37289_length_2246_cov_3.785942"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..1005
                     /locus_tag="PLJ64_12485"
     CDS             181..1005
                     /locus_tag="PLJ64_12485"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC94346.1"
                     /translation="MSTPFYKPRFLRVFTGLVYLYLFIPIVIVIVFSFNSAKSLQVMQ
                     GVSLRWYSDFFNDPTLMDSLVTSVQIAVLTMVISTIVGTALAIGLVRAHSGTARGFNI
                     LMLVPLITPEIVAGISALLIFSQLGIGLSIWTIVIAHITFSISYVTIVVRGRLATIGV
                     EVEDAAQDLGASKWESVRLVLVPALWPAIVAAGLLVFALSFDDFVLSFFTTGEDAQPL
                     PVRIWSMIRFGVSPTINAVGTLMMVVSISAVALAVLIPRLFGRRESGVKVLTGAEE"
     gene            1007..2131
                     /locus_tag="PLJ64_12490"
     CDS             1007..2131
                     /locus_tag="PLJ64_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC94347.1"
                     /translation="MNGEGSPGPAAGAGEAIRIEGVTKRFGDFVAVDQVDLSIAQGEF
                     FSLLGPSGCGKTTTLRMLAGFEVPSEGRILLEGEPVENVPPHQRNVNMVFQSYALFEH
                     LDVNGNVAFGLKRKKVPKEEITTRVAEALELVDLSSRASARPNELSGGQRQRVALARA
                     LVNRPRVLLLDEPLGALDLKLRRQMQIELKAIQREVGITFVYVTHDQEEALSMSDRIA
                     VMSDGRVAQCGPPEEVYEHPKEEFVAGFIGISNLLEGRVETGGRVRIGEDCLEVPDLG
                     DGRGTGETVQIAVRPEKVAVDEIGVEPYRIEPEMFTVEGEIESKVYLGVGNQLTLKLA
                     GGGRLVALEQATYRMRAEEQWEVGQRVRIGWHPEHCMVLS"
BASE COUNT          355 a          717 c          767 g          407 t
ORIGIN      
        1 cctacatggt cggcaacctg atccaggacc agttcttcgc cgcctcgaac tggcccttcg
       61 gctcggccct gaccgtgatc atgatggcct tcctcgcgat ctggatggtc ttctacctgc
      121 ggcgcgccag caagggcgag gcggaggttc tgtgagcgca agcgaaacca aaggctgccg
      181 ttgagcacgc cgttttacaa gccgcgcttc ctgagggtct tcaccgggct ggtctacctc
      241 tacctgttca tcccgatcgt catcgtgatc gtcttctcgt tcaacagcgc gaagtcgctc
      301 caggtgatgc agggggtctc gctccgctgg tactcggact tcttcaacga ccccaccctg
      361 atggattcgc tggtcacctc ggtgcagatc gcggtcctga ccatggtcat ctcgaccatc
      421 gtcggtaccg cactcgcgat cggcctggtc cgggcccatt ccggcaccgc caggggcttc
      481 aacatcctga tgctggttcc gctgatcacc ccggagatcg tggccggcat ctcggcgctc
      541 ctgatcttct cccagctcgg gatcggcctt tcgatctgga caatcgtgat cgcccacatc
      601 accttctcga tctcctacgt gacgatcgtg gtccggggga ggctggcgac gatcggggtc
      661 gaggtcgagg acgccgccca ggacctcggg gcaagcaagt gggaatcggt acggctggtg
      721 ctggtgccgg ccctctggcc ggcgatcgtc gccgcggggc tgctcgtctt cgcgctttcc
      781 tttgacgatt tcgtgctcag cttcttcacc accggcgagg atgcccagcc gctgccggtg
      841 cggatctggt cgatgatccg gttcggggtc tcgccgacga tcaacgcggt cggcacattg
      901 atgatggtcg tctcgatcag tgcggtcgcc ctggcggtgc tgatcccgag gctgttcgga
      961 cgccgcgaga gcggggtcaa ggttctgacc ggagcggagg agtgagatga acggagaagg
     1021 cagccccggc ccggcggcgg gcgccggcga ggcgatcagg atcgaagggg tgaccaagcg
     1081 cttcggtgac ttcgtcgcgg tcgatcaggt cgacctctcg atcgcccagg gcgagttctt
     1141 ttcgctgctc ggcccgtccg gctgcggcaa gaccacgacc ctgcggatgc tggccggctt
     1201 cgaggtcccg agcgagggcc ggatcctgct cgagggcgag ccggtcgaga acgtgccgcc
     1261 ccaccagcgg aacgtcaaca tggtcttcca gagctacgcg ctcttcgagc acctcgacgt
     1321 caacggcaac gtcgccttcg gcctgaagcg caagaaggtg ccgaaggagg agatcacgac
     1381 ccgggtcgcc gaggcgctcg agctggtcga cctttcctcc cgcgcgtcag cccggccgaa
     1441 cgagctctcc ggcggccagc ggcagcgggt cgcgctggcc cgggcgctgg tcaaccggcc
     1501 gcgggtgctg ctgctcgacg agccgctcgg cgcgctcgac ctgaaacttc gccgccagat
     1561 gcagatagaa ctgaaggcga tccagcggga ggtcgggatc accttcgtct atgtgaccca
     1621 cgatcaggaa gaagcgctct cgatgtctga ccggatcgcc gtgatgagcg acggccgggt
     1681 cgcccagtgc gggccgccgg aagaggttta cgagcatccg aaggaggagt tcgtggccgg
     1741 gttcatcggc atctcgaacc tgctcgaagg cagggtggaa accggcggcc gggtccggat
     1801 cggcgaggac tgcctcgagg tgcccgacct cggtgacggt cgcggcaccg gtgaaacggt
     1861 ccagatcgcg gtgcggccgg agaaggtcgc ggttgacgag atcggggtcg agccctaccg
     1921 gatcgagccc gaaatgttca cggtcgaagg cgagatcgaa agcaaggtct acctcggggt
     1981 cggcaaccag ctcaccctga agctggcggg cggcggtcgg ctggtcgccc tcgagcaggc
     2041 gacctaccgg atgcgggcgg aggagcagtg ggaggtcggc cagcgcgtca ggatcggctg
     2101 gcaccccgaa cactgcatgg tcctctccta gggttcgtca gcggccttcg taggtcgcct
     2161 tgccggggcc gttctcgagg aaggagtcga ccgcgttctt gaggtcctcc gactcgaaga
     2221 gttcggccgc gtcgcgcctg gtgacc
//
LOCUS       DAOQKN010000283         2244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_37350_length_2244_cov_4.703061, whole genome shotgun sequence.
ACCESSION   DAOQKN010000283 DAOQKN010000000
VERSION     DAOQKN010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37350_length_2244_cov_4.703061"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1087
                     /locus_tag="PLJ64_12495"
     CDS             <1..1087
                     /locus_tag="PLJ64_12495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HNC94348.1"
                     /translation="GRGGGRYGSSGLKDRFGRGGSGRSKGGKRWLKWILFACLGWFCL
                     SVLAFAVSAQLQSMKLDGDAKAALGGSPWILTSPQNVLIIGTDSRPPDTLEPGAAQEE
                     RCYEQQAEGAAPHDGCAGARADTLMVVRAGGGKFKKLSIPRDSYAAIPGQDPQKINGA
                     YAFGGAALQIQTVEDFLGITIDHVVILNFTGFEDFIDAIGGVKVDVPVRLCADISGGE
                     ANGGWSIDLEKGSNTLDGETALAYARTRKPSPCPGSEPSAYTEGYDDLDRAAAQQLVM
                     NGIKQRLTDPLRLPYNFIKGPIIGWTAPKAFVSDMGFFTLPQLALSTVFAGSSKPDVL
                     VPSGPGPGGSLEIPESERQRAAAELEG"
     gene            complement(1232..1639)
                     /locus_tag="PLJ64_12500"
     CDS             complement(1232..1639)
                     /locus_tag="PLJ64_12500"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNC94349.1"
                     /translation="MLCFDSSALVKCYQEEEHSPWVREIMASDPDWCGSALLAAEGPI
                     ALSRSVPEADDLQVADARISRDLGFFFLVAVDADCLVRAVDIGRDFGLRTLDAIHLAA
                     ARILPSECSFVTFDDRQREAAESLGLKVLTPPV"
     gene            complement(1639..1923)
                     /locus_tag="PLJ64_12505"
     CDS             complement(1639..1923)
                     /locus_tag="PLJ64_12505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HNC94350.1"
                     /translation="MDRIGIRELRADLSNQVKRAAAGEAVVVTIDGEPKALLTPFVAE
                     SGEMTLEELIRTGRVIPGPRVNEPRPPRRQGIKGGRPTKDVLDEIREDKI"
     gene            complement(1967..>2244)
                     /locus_tag="PLJ64_12510"
     CDS             complement(1967..>2244)
                     /locus_tag="PLJ64_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94351.1"
                     /translation="CGAPVQKVLHSPAIHFKGSGFHNTDYGTRKSNKAKAAAESAKKD
                     SGSSSSSSGSSDSGSSSSGSSDSGSGSSSSSSSDSGSKASSGSSNSD"
BASE COUNT          418 a          769 c          677 g          380 t
ORIGIN      
        1 cggccggggt ggcggtcggt acggatcttc cggcctcaag gatcgcttcg ggcgtggagg
       61 ttccggccga agcaagggcg gcaaacggtg gctcaaatgg atcctcttcg cctgcctcgg
      121 ctggttctgc ctgagcgtgc tcgccttcgc cgtttccgcc cagcttcagt cgatgaaact
      181 cgacggtgac gccaaggcag ccctcggcgg cagcccctgg atcctgacca gcccccagaa
      241 cgtcctgatc atcggcacgg attcccgccc cccggacacg ctcgaaccgg gggccgccca
      301 ggaggagcgc tgctacgaac agcaggccga gggcgcggca ccgcatgacg gctgcgccgg
      361 cgcccgggcc gacaccctga tggtggtccg cgccggcggc ggcaagttca agaagctgag
      421 catcccgcgc gacagctacg cggcgattcc cggccaggat ccgcagaaga tcaacggtgc
      481 ctacgccttc ggcggcgccg ccctccagat ccagacggtc gaggatttcc ttggaatcac
      541 gatcgaccac gtggtgatcc tcaacttcac cggctttgag gacttcatcg acgcgatcgg
      601 cggggtcaag gttgacgtgc cggtccgcct ttgcgccgac atttccggcg gcgaggccaa
      661 cggaggctgg tcgatcgacc ttgagaaggg aagcaacacg ctcgacgggg agaccgccct
      721 cgcctacgcc cggacccgca agcccagccc ctgcccgggt tcggagccaa gcgcctacac
      781 cgagggttac gacgacctcg accgggccgc cgcccagcag ctggtcatga acggcatcaa
      841 gcagcggctg accgatccgc tccgcctgcc gtacaacttc atcaagggcc cgatcatcgg
      901 ctggaccgcc cccaaggcat tcgtctccga catgggcttc ttcaccctcc cccagctcgc
      961 cctctccacc gtcttcgccg gctcctcaaa gcccgacgtg ctggttccct ccggccccgg
     1021 cccgggcggc agcctcgaaa tccccgagtc agagcgccag cgcgccgccg ccgaactcga
     1081 gggctagaac ccgccgcgag gcggcccgat gtcaccgagt gcccactttg tgccacatac
     1141 cggcacaaag tgggcattcg acgccagacc ccgccatacc gtccgaaatc gctgaacctg
     1201 ccagatccct gaagcgcggt agccgcgctg gctagaccgg cggagtaagg accttcaggc
     1261 cgagggattc ggccgcttcg cgctggcggt cgtcgaaggt gacgaaactg cattcggacg
     1321 ggaggattct ggccgccgcc aggtggattg cgtccagggt tctcaagccg aaatcccgac
     1381 cgatgtcgac tgcccgaacg aggcagtccg cgtcgactgc cacgagaaaa aagaagccca
     1441 gatccctgct gatcctggcg tcagcgacct gcaaatcgtc tgcttcgggt acggagcgag
     1501 acagcgcgat cggtccttcc gccgccagca gagcggaacc acaccaatca gggtcgctcg
     1561 ccatgatttc gcgaacccac ggtgaatgct cttcctcctg gtagcacttg accagagccg
     1621 acgaatcgaa gcacaacatc agatcttgtc ctcgcggatc tcgtccagaa catccttcgt
     1681 cggacgccct cctttgatcc cttgccgtcg aggtggtctg ggctcgttga ctctcggtcc
     1741 gggaattacc cgaccggtcc tgatcagctc ttccagagtc atctcaccag actccgcgac
     1801 gaacggtgtc agaagggctt tcggctctcc atcgatcgtg acgaccacag cttcaccggc
     1861 ggcagcacgc ttcacctgat tgctcaagtc cgcgcggagt tccctgatac caatccgatc
     1921 catatagcga gtgtacacga cggtacaccc gaaaaaccga ccttgctcag tcggagtttg
     1981 aagaacccga cgaggctttt gaaccggagt ccgaagagct cgacgagcta gacccggaac
     2041 cggaatcgga tgagccggaa ctcgaagatc ccgagtcgga ggagcccgac gagctggagc
     2101 tcgagccgct gtccttcttc gctgattcgg ctgcggcctt cgccttgttg gacttgcggg
     2161 tgccgtagtc ggtgttgtgg aaaccggaac ccttgaagtg gatcgccggt gaatgcagca
     2221 ccttctggac gggggcgccg catt
//
LOCUS       DAOQKN010000284         2188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_39123_length_2188_cov_3.617440, whole genome shotgun sequence.
ACCESSION   DAOQKN010000284 DAOQKN010000000
VERSION     DAOQKN010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39123_length_2188_cov_3.617440"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="PLJ64_12515"
     CDS             complement(<1..794)
                     /locus_tag="PLJ64_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HNC94352.1"
                     /translation="MAFSEENFPKAVRIREVGPRDGFQNEPEVIPTEEKIRLINMLGQ
                     TGLRRIEVTSFVRPDVIPQLSDGVEVLNGIDRPEGVSYSVLIPNRRGLENALDLRDRF
                     DETNFFLSASETHNRKNVNRSVEESMSGLKETIPVAVEAGLRCEGVISTSFGCPYEGD
                     VPVENVLALALRLARAGCVEVGFGDTTGMANPVQVRRFFSTVRERFKQEGLGDTELTA
                     HFHDTRGQGLANVLAALEEGIDSFESSFGELGGCPVPPGATGNIAS"
     gene            complement(806..1177)
                     /locus_tag="PLJ64_12520"
     CDS             complement(806..1177)
                     /locus_tag="PLJ64_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94353.1"
                     /translation="MSIAEKTGTEREENFTVEGMLMTDVGGLLETGVLSTPGMIGMME
                     RNCALLVQEFLPEGSATVGFEVNVKHVSGAGEGAECTAWGRLDEVIDERKFRFSVEVR
                     EGDRVLGVGTHERRAITFNVA"
     gene            1223..1918
                     /gene="npdG"
                     /locus_tag="PLJ64_12525"
     CDS             1223..1918
                     /gene="npdG"
                     /locus_tag="PLJ64_12525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HNC94354.1"
                     /translation="MSENSESPVIPVIGGTGALGSGLAKRWAAGGARVVIGSRDAGRA
                     AEAAAKIAEETGGTVEGMSNEDAAKAADIVFLTIPFRNQSEYLTNLKGSLTEGQILVD
                     CSVPLAAAISGKATRTIGVWQGSAAEQAQEMVPDGVTVVSALHTVSAPGLADLSHDLD
                     EDVPVCGDRKADKARVAELISRIEGLRPVNAGALEISRIVEQITALMISVNIRYKTHA
                     GLKFTNLPEGETF"
     gene            1918..>2188
                     /locus_tag="PLJ64_12530"
     CDS             1918..>2188
                     /locus_tag="PLJ64_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HNC94355.1"
                     /translation="MKVALLAGGTGGAKLAAGMQAVVGDGLTVIANTGDDIETLGVYV
                     SPDPDLVTYWLSGQVDEVRGWGIKDDGFDVFQRMARFGAPDWFGLS"
BASE COUNT          403 a          718 c          685 g          382 t
ORIGIN      
        1 tccgatgcga tgttcccggt tgcgccgggc ggaacaggac agccaccgag ctcgccgaag
       61 ctcgactcga acgagtcgat gccttcctcc agcgctgcga ggacattggc gagaccctgt
      121 ccgcgggtgt catggaagtg cgcggtcagc tccgtatccc ccagcccctc ctgcttgaag
      181 cgctcgcgga ccgtcgagaa gaagcgccgg acctgaaccg ggttggccat gccggtcgtg
      241 tccccgaacc caacttccac gcagcccgcc ctggccagcc gcagggccag cgcgagcacg
      301 ttctcgaccg gcacatcgcc ttcgtaggga cagccgaagg aagtggaaat caccccttcg
      361 cagcggagcc cggcttccac agccaccggg atcgtctctt tcagaccgct catcgactcc
      421 tcgacgctgc gattgacgtt cttgcggttg tgtgtctcgg aagcggagag gaagaagttg
      481 gtctcgtcga agcggtcgcg aagatcgagt gcgttctcga gcccacgacg gttcgggatc
      541 agtaccgagt aggagacacc ttcgggacga tcgatgccgt tcaggacttc aaccccatcc
      601 gagagctgcg ggatcacgtc gggccgaacg aaggaagtga cctcgatccg cctcaggcca
      661 gtctggccga gcatgttgat cagccggatc ttctcctctg tcgggattac ttcgggctcg
      721 ttctggaagc cgtcacgcgg tcctacctcg cggatcctga ccgccttggg gaagttctct
      781 tcgctgaagg ccatgggttc cccgctcagg cgacattgaa ggtgatcgcg cgacgttcgt
      841 gggttccaac cccgagaacc ctgtcgcctt cgcggacctc aaccgagaag cggaacttgc
      901 gctcgtcgat tacctcgtcg aggcgacccc aggcggtgca ctcggcccct tcacccgctc
      961 cggagacatg cttgacgttc acctcgaagc cgaccgtggc cgatccttcg ggcaggaact
     1021 cctggaccag aagggcacag ttccgctcca tcattccgat catcccgggg gtcgagagca
     1081 ccccggtctc gagaagaccg ccgacgtcgg tcatcagcat cccctcgacg gtgaagtttt
     1141 cctcgcgctc ggtcccggtc ttctcggcaa tcgacatggg ccaagaatag ttgggctgcg
     1201 gcttgaccta gactgcgacc ccgtgagcga gaacagcgaa tcacctgtca tccctgtaat
     1261 cggcggaacg ggagcgctcg gctccggcct cgccaaacgc tgggctgccg gaggcgccag
     1321 ggtcgtgatc ggctctcgtg acgccggccg tgcggcagag gccgcggcaa agatcgccga
     1381 agagacgggt ggaacggtcg aaggcatgtc caacgaggat gccgcgaaag ccgccgacat
     1441 cgtctttctc accattccct tccggaacca gtccgagtac ctgaccaatc tcaagggatc
     1501 gctgaccgaa ggtcagatcc tggtcgactg ctcggtacct cttgccgcag cgatcagtgg
     1561 caaggcgacc cgaacgatcg gcgtctggca aggctccgcg gccgaacagg cgcaggagat
     1621 ggtccccgac ggggtgactg tggtctccgc tctccacacc gtcagcgccc ccggactcgc
     1681 cgacctgtca catgaccttg acgaggacgt gccggtctgt ggcgacagga aggccgacaa
     1741 ggcgagggtc gccgaactga tttcgaggat cgaaggcctg cgcccggtaa atgccggcgc
     1801 cctcgagatc tcgcggatcg tcgaacagat caccgccctg atgatctcgg tcaacatcag
     1861 gtacaagacc cacgccgggc tcaagttcac gaacctcccc gagggcgaga ctttctgatg
     1921 aaggtcgccc tgctggccgg gggcactggc ggggccaagc ttgctgccgg gatgcaggcg
     1981 gtggtcggcg acgggctgac cgtgatcgcc aacaccggcg acgacatcga gaccctcggc
     2041 gtctacgtct cacccgaccc ggacctggtc acctactggc tgtccggaca ggtggacgaa
     2101 gtgcggggct ggggcatcaa ggacgatggt ttcgacgtct tccagcggat ggcacgattc
     2161 ggagcccccg actggttcgg actctcca
//
LOCUS       DAOQKN010000285         2183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_39268_length_2183_cov_6.165414, whole genome shotgun sequence.
ACCESSION   DAOQKN010000285 DAOQKN010000000
VERSION     DAOQKN010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39268_length_2183_cov_6.165414"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1462)
                     /locus_tag="PLJ64_12535"
     CDS             complement(<1..1462)
                     /locus_tag="PLJ64_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNC94356.1"
                     /translation="MADSRNLLRARQFFSPERSAEGWSETSLRPRDWEQGYRDRWQHD
                     RIVRSTHGVNCTGSCSWKIHVKDGLVTWETQQVDYPSNGAETPDYEPRGCPRGASFSW
                     YVYSPLRPKFPYVRGELLSMYREARQRLGDPVEAWASIVDDPEKARAYKSRRGKGGFL
                     RASWNEVTEIAAAAHVHTIKKYGPDRIAGFSPIPAMSQVSYAAGSRFLSLIGGVPLSF
                     YDWYADLPPASPQVWGDQTDVPESADWWDAAYLIMWGSNIPQTRTPDAHFMTEARYRG
                     QKTIVVSPDYAGNTKFADQWLPATAGTDGALAMAMGHVILKEFFVDRQAEYFDGYARQ
                     FTDMPFLVELEDHGDGTVHGGPMLRASDLGETDENSEWKTVLVDSRTDQAVVPNGTIG
                     ERWGEEGEGKWNLDLEGIEPALTLLGRHDELVEVTLPRFDGGETEGGTTMVRGVPARR
                     IGGKLVTTVFDLTLAQYGVAREGLPGTWPKDYSDPAE"
     gene            complement(1678..>2183)
                     /locus_tag="PLJ64_12540"
     CDS             complement(1678..>2183)
                     /locus_tag="PLJ64_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HNC94357.1"
                     /translation="DTLSIVADRSLDEVMAPDILVIPGGFGTRALLDHEPLLDWIRAV
                     DETTTWTTSVCTGALLMAAAGLLDGRPATTHWLARETLAGLGANPVPDRVVFDGKYVT
                     GAGVSAGIDMALTLVTEIYGPEIAQAVQLGIEYDPQPPHDAGAPEKAAPEIVDVVTAV
                     FTAAETA"
BASE COUNT          330 a          703 c          769 g          381 t
ORIGIN      
        1 gctcggccgg gtccgagtaa tccttgggcc aggtgccggg caggccttcg cgggcaaccc
       61 cgtactgggc gagggtcagg tcgaagaccg tggtgaccag cttgccgccg attcgccggg
      121 ccggcacgcc gcggaccatc gtggtgcccc cctcggtctc gccgccgtcg aagcgcggca
      181 gggtcacttc gaccagttca tcgtggcggc cgagcagggt cagggccggc tcgatgcctt
      241 cgaggtcgag gttccacttg ccctcgcctt cctcgcccca gcgctcgccg atcgtcccgt
      301 tcgggaccac tgcctgatcg gtccgggagt cgaccaggac ggtcttccac tcggagttct
      361 catcggtttc gccgaggtcc gaggccctca gcatgggacc gccgtggacg gtgccgtcac
      421 cgtggtcttc gagctcgacc aggaagggca tgtccgtgaa ctggcgtgcg tagccgtcga
      481 agtactcggc ctgccggtcg acgaagaact ccttgaggat cacatggccc atcgccatcg
      541 ccagggcgcc atcggtgccg gcggtggcgg gcagccactg gtcggcgaac ttggtgttgc
      601 cggcgtagtc gggcgagacc acgatcgtct tctggccgcg gtagcgggcc tcggtcatga
      661 agtgggcgtc gggcgtgcgg gtctgcggga tgttcgaacc ccacatgatc aggtaggcgg
      721 catcccacca gtccgctgac tcgggcacgt cggtctggtc tccccagacc tgcggcgagg
      781 ccgggggcag gtcggcgtac cagtcgtaga agctgagcgg cacaccgccg atcagggaga
      841 ggaagcggct gccggcggcg taggagacct gggacatggc cgggatcggg ctgaagccgg
      901 caatgcggtc gggcccgtac ttcttgatcg tgtgaacgtg ggccgcggcg gcgatctcgg
      961 tgacctcgtt ccagctggcg cgcaggaagc cgcccttgcc ccggcgtgac ttgtaggccc
     1021 gggccttttc cgggtcatcg acgatcgagg cccaggcctc gaccgggtcg cccaggcgct
     1081 gtcgcgcctc gcggtacatc gaaaggagtt cgccacgcac gtacgggaac ttgggccgaa
     1141 gcggcgagta gacgtaccag gaaaaggagg cgccgcgcgg gcatccccgt ggctcgtagt
     1201 cgggcgtctc ggcaccgttg gaggggtagt cgacctgctg ggtctcccag gtgaccagcc
     1261 cgtccttgac gtggatcttc caggaacagg aaccggtgca gttcaccccg tgggtcgaac
     1321 gaacgatccg gtcgtgctgc cagcgatcgc ggtagccctg ttcccagtcc cgcggcctga
     1381 gcgaagtttc gctccagcct tcagcggatc gttccgggct gaagaactgc cgggcgcgaa
     1441 gcaggttgcg tgagtcagcc acgagtggcg tctccggcgc aagattggtg ggaactccga
     1501 ggtgggatca tttccccttc cggtgacggt tcagacttgt tgcgccaaca tattggattg
     1561 aacggtcgct attccagtac ttttccgcag acttcctgcg ggttgctcac actcaattgt
     1621 tgccctgccg gcggggctcc caaaccggcg gcggcggggt cacccgtcgc gtgtcactca
     1681 ggcggtttcg gccgccgtga aaacggccgt gaccacgtcc acgatctccg gtgcggcctt
     1741 ttcgggtgca ccggcatcgt gcgggggctg cgggtcgtat tcgatcccga gctggaccgc
     1801 ctgggcgatc tccgggccgt agatctcggt gaccagggtc agagccatgt cgatgccggc
     1861 cgaaacgccc gcaccggtca catacttccc gtcgaagacc acccggtcgg gaaccggatt
     1921 cgcaccgagg ccggccaggg tttcacgggc aagccagtga gtggtcgccg ggcggccgtc
     1981 gagcagtccg gcggcggcca tgagcaaagc gccggtgcaa acggaggtcg tccaggtcgt
     2041 cgtctcgtcc accgcgcgga tccagtcgag gaggggctcg tgatccagca gcgcccgggt
     2101 tccgaagccg ccggggatta ccaggatgtc cggcgccatc acctcgtcga gggacctgtc
     2161 cgcgacgatc gagagcgtgt ccg
//
LOCUS       DAOQKN010000286         2182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_39302_length_2182_cov_5.096380, whole genome shotgun sequence.
ACCESSION   DAOQKN010000286 DAOQKN010000000
VERSION     DAOQKN010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39302_length_2182_cov_5.096380"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1585
                     /locus_tag="PLJ64_12545"
     CDS             <1..1585
                     /locus_tag="PLJ64_12545"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNC94358.1"
                     /translation="RNSLGSAFDLGPGDSLLLKRGTTCHGRLEVTGGGSEIQRARVGA
                     YGSGELPQLNGTGRDAVLLVDTSNLTVGDLDISNPGTGGPIGEGDQIRNGVRVIAQAG
                     TVSDLTIGGLEIHDVDGDLTKTPEGSAAIQISATGAAPVRFTGLHITGNQITSVSRSA
                     ISISGSNDLDRPEASQAWPEASSGVVIKDNRIDQVAGDGIVTRGTDGALIEGNVVSHG
                     NLAGRPLLDPEGPMCNAGIWAFRANNTVIRGNEVFGMEHNGCDGTGFDVDYRQDGTVI
                     EGNYSHDNEGGFVLLCTDTATHRADVRFNLSVDDGTMINHGPCGIADGILGDLSGIRM
                     FNNTVVGDSPTTSIQLAVSDEMYQPGRFLFRNNLVYARQPAGQIACGNDCTHNAFFNL
                     PPSGTHAVTADPQLIAGYRLGPKSPLRGAGMPITGGATTDYFGNPVPATPSIGFDQTP
                     ASRPAQPTPACRKARKARDRTIERAADLNRKLRKLRSKHAPRRQTRSVASRLGKARGE
                     VKRLTRVARRACRPKGGAS"
     gene            complement(1582..>2182)
                     /locus_tag="PLJ64_12550"
     CDS             complement(1582..>2182)
                     /locus_tag="PLJ64_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94359.1"
                     /translation="TGRYLYGDLCDQALRTLDLDAGGGDPQPAGFSIPGDGGNLRSFG
                     EDAKGCVYVLSNVAVYRVAPDGSTAPACPGTIEPPPTVTYNSYIPHQQVIGKRLIVAA
                     KCSIACTGSATAKVRITRNRFRKEPVIFNLKTPTLNLAANTRTSLVFRVPVKRVTAMK
                     KAARNGSRVTAWVKVSLTGEDGSGGSGSSSIRLVRPKRR"
BASE COUNT          393 a          749 c          731 g          309 t
ORIGIN      
        1 acgcaactcg ctcggcagtg ccttcgacct cggtcccggc gacagcctgc tgctcaaacg
       61 cggcacgacc tgccacggcc ggctcgaggt caccggtgga ggcagcgaaa tccagcgggc
      121 cagggttggc gcctacggct cgggcgagct cccgcagttg aacggaacgg gccgcgacgc
      181 ggtgctcctc gtggacacct cgaacctgac ggtcggggat ctcgacatct ccaatcccgg
      241 aacaggcggc ccgatcggtg agggcgatca gatccgcaac ggagtgcggg tgatcgcgca
      301 ggcgggaacc gtcagtgacc tcacgatcgg cggtctggag attcacgacg tcgacgggga
      361 cctgaccaag acgccggaag ggagcgccgc gatccagatc agcgcgaccg gcgccgcgcc
      421 ggtccggttc acgggcttgc acatcaccgg caaccagatc acctcggtgt cgcggagcgc
      481 gatctcgatc tccggcagca acgacctcga ccggccggaa gccagccagg cctggccgga
      541 agcttcctcc ggagtggtga tcaaggacaa ccggatcgat caggttgccg gtgacggaat
      601 cgtcaccagg ggaaccgacg gcgccctgat cgaaggcaac gtcgtaagcc acggcaacct
      661 ggccggccgg cccctgctcg acccggaagg cccgatgtgc aacgcgggca tctgggcctt
      721 ccgggccaac aacaccgtga tccggggcaa cgaggtcttc ggcatggaac acaacggctg
      781 cgacggcacc ggcttcgatg tggactaccg gcaggacggc acagtgatcg agggcaacta
      841 cagccacgac aacgagggcg gcttcgtcct gctctgtacc gacaccgcga ctcaccgggc
      901 cgacgtgcgg ttcaacctga gcgttgacga cggcacgatg atcaaccacg gtccctgcgg
      961 gatcgccgac ggcatcctcg gcgacctttc cggcatccgg atgttcaaca acaccgtggt
     1021 cggcgacagc ccgaccacct cgatccagct ggcggtatcg gacgagatgt accagccggg
     1081 cagattcctc ttccggaaca acctggtcta cgcccgtcaa ccggccgggc agatcgcctg
     1141 cggaaacgat tgcacccaca acgccttctt caaccttccg cccagcggca cgcacgcggt
     1201 caccgccgac ccgcagctga tcgccggcta ccggctcggt cccaagtcac cgctgcgagg
     1261 agccggcatg ccgatcaccg ggggagccac caccgactac ttcggcaacc cggtcccggc
     1321 aacaccgtcg atcggttttg atcagacccc ggccagccgg cccgctcagc cgacgccggc
     1381 ctgccgcaag gcccgcaagg cccgggatcg gacgatcgaa agagcagcgg acctcaaccg
     1441 gaaactgagg aagctccgtt cgaagcacgc gccccgcagg cagaccagat cggtcgcaag
     1501 ccggctcggc aaggcccggg gcgaggtgaa gcgactgacc agggtcgccc gtcgtgcctg
     1561 ccggcccaaa ggcggcgcca gctaacggcg tttggggcgg accaggcgga tcgaactcga
     1621 gccgctgccg cccgagccgt cctcgccggt caggctgacc ttcacccagg cggtcacccg
     1681 gctgccgttg cgggcggcct tcttcattgc cgtgacccgc ttcaccggca cccggaaaac
     1741 gaggctggtg cgggtgttgg cggcaaggtt cagggttggc gtcttcaggt tgaagatcac
     1801 cggctccttg cggaaccggt tgcgggtgat ccggaccttc gcggtggccg acccggtaca
     1861 ggcgatcgag cacttcgcgg ccacaatgag gcgcttgccg atcacctgct ggtgcgggat
     1921 gtacgagttg taggtcacgg tgggaggcgg ctcgatcgtt ccggggcagg ccggggccgt
     1981 ggaaccgtcg ggtgcgaccc ggtagaccgc gacgttgctc agcacgtaga cgcagccctt
     2041 cgcgtcctcc ccgaaggagc ggaggttgcc tccgtcaccc gggatgctga atccggccgg
     2101 ctgcggatcg ccaccgccgg catcgaggtc gagggtccgg agcgcctggt cgcaaaggtc
     2161 tccgtagagg tagcggccgg tc
//
LOCUS       DAOQKN010000287         2176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_39513_length_2176_cov_3.759076, whole genome shotgun sequence.
ACCESSION   DAOQKN010000287 DAOQKN010000000
VERSION     DAOQKN010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39513_length_2176_cov_3.759076"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="PLJ64_12555"
     CDS             <1..800
                     /locus_tag="PLJ64_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94360.1"
                     /translation="VVPLVASVWSADPARMDEFPVGFLARFLDNHGLLSLNDRPRWRT
                     VKGGSRTYVDALTGPIAATIRTSTPVEWIARYGDSVMLKAGGGSPERFDRVIVATHSD
                     QALRMIAAPTGEEKSFLGAVPYQANEAVLHTDESVMPVRRAAWACWNYHLGHEAQGRT
                     ALTYDMNRLQSLKCRQRFFVSLNVTRRIDRSKVLARFHYEHPVFTPEGVRAQARWSEI
                     SGVDRIHYAGAWLRNGFHEDGVVSGIRAADQVLDLIEAAPQAAALAA"
     gene            797..1654
                     /locus_tag="PLJ64_12560"
     CDS             797..1654
                     /locus_tag="PLJ64_12560"
                     /inference="COORDINATES: protein motif:HMM:NF018770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HNC94361.1"
                     /translation="MNPVRESAIYEGRIRHRRFEPVTREFEYRIFMVYLDLDELPEVM
                     SVHPLWSTRPRAPARFKRSDYLGPAERPLKQAVLDRVESRCGRRPAGPVRMLTHLRYF
                     GVCFNPVTFYYCFDPFGQRLESVLAEVTNTPWGERHSYLVNRQRRPGVLAADVAKRLH
                     VSPLMAMDHRYELVFAEPGPTLSVHMASRRKGHLHFDATLRMERQQLTRAKLGRLLLT
                     RLPMPAKVLGGIHAEAARTWLAGARYHPHPEKTSREDLPNRSKDGNLCPVDHSSPTRP
                     QAERRRGEG"
     gene            1657..>2176
                     /locus_tag="PLJ64_12565"
     CDS             1657..>2176
                     /locus_tag="PLJ64_12565"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC94362.1"
                     /translation="MWRRLVLAVIGRLRRGRIEFIEGGRVTAIGEPGAEPLARVEVHS
                     PRAWRRMVRGSTGMAEGYIKREWDSDDLVALGTIAGLNLGQLDRIKRRFRFILWPFQK
                     LGPLMPRTTRRRGRKQIAAHYDLGNDLFSLFLDESMNYSAAVFRGDPDQTLGQAQIAK
                     MDQIADQLDLSPD"
BASE COUNT          366 a          736 c          709 g          365 t
ORIGIN      
        1 tcgtcgttcc cctcgtggcc tcggtctggt cggctgaccc cgcccggatg gatgaattcc
       61 cggttggctt tctggcccgc tttctggaca accacggcct gctttcgctc aatgaccggc
      121 ctcgctggcg gaccgtcaag ggtggctccc ggacctatgt cgatgcgctg accggtccga
      181 tcgccgcgac gattcggacc agtaccccgg tcgagtggat cgcccgctac ggcgattccg
      241 tgatgttgaa ggccggcggc ggctcaccgg agcggttcga ccgggtcatc gtggcgaccc
      301 actccgatca ggccctgagg atgatcgccg ccccgaccgg ggaggagaag tcgttccttg
      361 gtgccgtgcc ctaccaggcc aatgaagcgg tccttcacac ggatgagtcg gtcatgccgg
      421 tccgccgtgc ggcctgggcc tgctggaact atcacctcgg gcacgaagcg cagggccgca
      481 ccgccttgac ctatgacatg aaccgcctgc agagcctcaa gtgccgccag cggttcttcg
      541 tttccctgaa cgtgacccgc cggatcgatc gatcgaaggt gctggcccgg tttcactacg
      601 agcacccggt cttcaccccg gaaggcgtcc gggcgcaggc ccgctggagt gaaatcagtg
      661 gcgtcgaccg gattcactac gccggcgcct ggctgaggaa cggcttccat gaagacggcg
      721 tggtaagtgg catccgggcg gccgaccagg ttcttgacct gatcgaggcg gcgccgcagg
      781 cggcggcact ggcggcatga atcccgtccg ggaaagcgcg atctacgaag gccggatccg
      841 gcaccggcgc ttcgaaccgg tgacccggga gttcgaatac aggatcttca tggtctacct
      901 ggacctcgac gagctgccgg aagtcatgtc ggttcatccc ctctggtcga ccaggccgag
      961 agcacccgcc cgcttcaagc gctcggacta cctcggcccg gccgagcgac ccttgaagca
     1021 ggccgtgctg gacagggttg aaagccgctg cgggaggcgg cccgccggtc cggtccggat
     1081 gctgacccat ctgcgctact tcggggtctg cttcaacccg gtgaccttct actactgctt
     1141 tgaccctttc gggcagcggc ttgagtcggt gctggccgag gtcacgaaca ctccctgggg
     1201 tgagcgtcac agctacctcg tgaaccggca gcgccggccc ggtgtgctcg ccgccgacgt
     1261 ggccaagcgg ctccacgttt caccgctgat ggcgatggac caccgctacg aactcgtctt
     1321 cgccgagccc ggcccgacgc tctcggtcca catggcgagc cggaggaaag gccacctcca
     1381 tttcgatgcc accctgcgga tggagcgaca acagctgacc agggcaaagc tcggtcggct
     1441 gctcctcacc cggttgccga tgccggcgaa ggttctgggc ggcatccacg ctgaagcggc
     1501 ccggacctgg ctggccgggg cccgttacca cccccacccg gagaaaacct cccgggagga
     1561 cttgccgaat cggtcgaaag acggcaatct ctgtcctgtg gaccactctt ctcccacccg
     1621 gccccaagcc gaaaggcgaa ggggcgaagg ctgatcatgt ggcgtcgcct cgtgctggcg
     1681 gtgatcggac gcctccggag gggccgcatc gaattcattg aaggcggcag ggtgacggcg
     1741 atcggggagc ccggcgcgga gcccctggcc cgggtcgagg tccactcgcc cagggcctgg
     1801 cgaaggatgg tccggggcag caccgggatg gccgaggggt acatcaagcg ggagtgggac
     1861 agcgacgacc tggtcgcgct gggcacgatc gccggcctca atctcggtca gctggaccgg
     1921 atcaagagac gcttccgttt catcctctgg cccttccaga aactcggccc cctgatgccg
     1981 agaacgaccc gccggagggg aaggaaacag atcgccgccc actacgacct cggcaacgac
     2041 ctcttctcgc tgtttctgga cgaatcgatg aactactccg cggcggtctt ccggggtgat
     2101 cccgaccaga ccctcggaca ggcccagatc gcgaagatgg accagatcgc cgaccagctc
     2161 gacctctcgc cggaca
//
LOCUS       DAOQKN010000288         2175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_39538_length_2175_cov_4.614151, whole genome shotgun sequence.
ACCESSION   DAOQKN010000288 DAOQKN010000000
VERSION     DAOQKN010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39538_length_2175_cov_4.614151"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="PLJ64_12570"
     CDS             <1..868
                     /locus_tag="PLJ64_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94363.1"
                     /translation="IYAGEGAEGANKTFKYSGAAFSSTQRLMAELADQDTRLNQFVGN
                     TSKFVTQLAEVSPQLTELVTNSNIALGKIAENNESLSLALQELPPTLRQGNTTFANLR
                     AALDDVQPMIAASGRAADAGLANYLKNDLRPVLIRARPVFNDLALAAGRPGPDNDTSD
                     LLSQLIPLHGRAEPAVNAVVRALNANQEDVSEMRAYAPDIFGSFSKIAGLLGSYDANG
                     PFAKVTPTATGVFQLNGTAIQPADTAIYGGLDVITGINRCPGGGVQAIAGSNPFLDNG
                     NLTGKCDPSQVP"
     gene            844..>2175
                     /locus_tag="PLJ64_12575"
     CDS             844..>2175
                     /locus_tag="PLJ64_12575"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNC94364.1"
                     /translation="MRPLTGTVMLKRIITILLVILAIVAVVLLVRSCNDDSDAYEVRA
                     VFDNGAFVVPGIDVRVAGANVGSVSSVDVSRPGEETGTDVPGKAIVVMNITDEGFQDW
                     KDDASCIIRPQSLIGEKFLDCQVTQPRPAGSEPPPSLDQIPDGQPGAGEYLLPIQNNA
                     QSVDLDLINNIYRLPYAQRFRIILNELGAGLATRGEDLRETVERANPALLQVNKVLAI
                     LASENKRLAQLAKDGERNMTALAAKKENMINFFKNAGYTAAASAERGQDLQQNLKNLP
                     ETLRQLRADFNAVGTFADRATPVFQYLKPAASDISEVQTKLGPFADAGRISITSLGRA
                     TRTAGPDLVASKPIINKLGDLSRKAYSPSANLSFFLKTLDQGGGFDNLMEVIYNTSSL
                     LNGYDQYGHYQRTNLIVTGCSTYANTAVGTCAGKWQPSSASSADLRAGLGDL"
BASE COUNT          415 a          779 c          637 g          344 t
ORIGIN      
        1 gatctacgcc ggcgaagggg ccgaaggcgc aaacaagacc ttcaagtaca gcggcgccgc
       61 cttctccagc acccagcggc tgatggccga gctggccgac caggacacga ggctcaacca
      121 gttcgtgggc aacacctcga agttcgtgac ccagctggcc gaggtatccc cgcagctgac
      181 cgagctggtc accaactcga acatcgcgct cggcaagatc gccgagaaca acgagtcact
      241 ctcactggcg cttcaggaac tgccgccgac cctgcgccag ggcaacacca ccttcgccaa
      301 cctgcgggcg gcgctcgacg acgtccagcc gatgatcgcg gcctccggtc gtgcggccga
      361 cgcggggctg gccaactacc tgaagaacga cctgcggccg gtcctgatcc gggcccgtcc
      421 cgtcttcaac gatctcgcgc ttgcggccgg gcgccccggg ccggacaacg acacatccga
      481 cctgctctcg cagctgatcc cgctgcacgg cagggccgag ccggccgtga atgccgtggt
      541 tcgcgccctg aacgcgaacc aggaagacgt ctcggagatg cgggcctacg cgccggacat
      601 cttcggctcc ttcagcaaga tcgccggcct gcttggcagc tatgacgcca acggtccgtt
      661 cgccaaggtc actccgaccg cgaccggcgt gttccagctc aacggcaccg cgatccagcc
      721 ggccgacacc gcgatctacg gcggcctcga cgtaatcacc ggcatcaacc gctgcccggg
      781 tggcggcgtc caggccatcg ccggctcgaa ccccttcctc gacaacggca acctgaccgg
      841 caaatgcgac ccctcacagg taccgtgatg ctgaagcgaa tcatcacaat cctgctcgtg
      901 atcctggcga tcgttgccgt cgtcctgctg gtacgcagct gcaatgacga cagcgatgcc
      961 tatgaggtcc gggcggtctt cgacaacggc gccttcgtgg tacccgggat cgacgtccgc
     1021 gtggccggtg ccaacgtcgg cagcgtcagc tcggtcgatg tttcgcggcc cggcgaagag
     1081 acgggtacgg acgtgcccgg caaggcgatc gtggtgatga acatcaccga cgagggcttc
     1141 caggactgga aggacgatgc ctcctgcatc atccggccgc agtcgctgat cggcgagaaa
     1201 ttcctcgact gccaggtgac ccagccccgt cccgccggca gcgagccacc accctcgctc
     1261 gaccagatcc ccgacggtca gcccggtgcc ggcgaatatc tgctgccgat ccagaacaac
     1321 gcccagtcgg ttgacctcga cctgatcaac aacatctacc gcctgccgta cgcgcagcgt
     1381 ttccggatca tcctcaacga actgggagcc ggtctggcga cccgcggcga ggatcttcgg
     1441 gaaacggtgg agcgggccaa cccggcgctt ctccaggtca acaaggttct ggccatcctc
     1501 gcttcggaga acaagcgcct ggcccagctc gccaaggacg gcgaaaggaa catgaccgcc
     1561 ctggccgcga agaaggagaa catgatcaac ttcttcaaga acgcgggata cacggcggca
     1621 gccagcgccg aacgcgggca ggacttgcag cagaatctca agaacctgcc cgagaccctg
     1681 cgtcagcttc gggccgattt caacgcggtc ggtaccttcg ccgatcgggc gactcctgtc
     1741 ttccagtacc tcaagccggc tgcctccgac atcagcgagg tccagaccaa gcttggcccc
     1801 ttcgcggacg ctggtcgcat ctcgatcacc agcctcggcc gcgccacccg gaccgccggt
     1861 ccggacctgg ttgcctcgaa accgatcatc aacaagctcg gcgatctctc ccgcaaggcc
     1921 tactcgcctt cggcaaacct cagctttttc ctcaagaccc tcgatcaggg tggcggcttc
     1981 gacaacctga tggaagtcat ctacaacact tcctcgctgc tcaacgggta tgaccagtac
     2041 ggccattacc agcgcaccaa cctgatcgtg accggctgct cgacctacgc gaataccgcg
     2101 gtgggtacct gcgccggcaa gtggcagccc tcttcggctt cgtcggcgga tcttcgcgcc
     2161 ggcctcggcg acctc
//
LOCUS       DAOQKN010000289         2148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_40385_length_2148_cov_5.436216, whole genome shotgun sequence.
ACCESSION   DAOQKN010000289 DAOQKN010000000
VERSION     DAOQKN010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40385_length_2148_cov_5.436216"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..896)
                     /locus_tag="PLJ64_12580"
     CDS             complement(129..896)
                     /locus_tag="PLJ64_12580"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HNC94365.1"
                     /translation="MSKATLFSLPGSHPAVSVKLMLDYKGIDFKTIDLMPLMHKAVVR
                     AAGFPGTTVPALKFNGEKLQGSVDIARALDRIVPEPPLLPADEDARKQVLMAEAFGEA
                     ELQHAVRQILWNAVRRQPKSIATFLEGSSLPLPPKVAAYTSGPIIWGEYKVNESTDEN
                     VKANLIALPEWLDRLDAWVADGTLGGATPNAADFQIATSIRLAATLQDLRPFLENRPI
                     YEAAVRTAPVYAGDVPPGLPTEWLAPLRENAAAPVSD"
     gene            940..1707
                     /locus_tag="PLJ64_12585"
     CDS             940..1707
                     /locus_tag="PLJ64_12585"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNC94366.1"
                     /translation="MLLAIDVGNTQTHLGAFEGNELTRHWRFQTRAGATSDELAERIS
                     GLMELRGTGFDDLDGLVVSSVVPPLGSEYTRLAQQYLDVGCLLVGPGIRTGMSIEIDN
                     PAEVGADRLVNAIAAYDEVGDVCVGVDFGTGINFDAVSADGAYLGGAIAPGVEISLTA
                     LTERAARISRIDLGEPKKAIGTSSREAIQVGVLYGFAGLVDGIARRINRELGGDARFI
                     ATGGYAKSIVPFSEEIDEIDELLTLKGLRIIHERNRG"
     gene            1688..>2148
                     /locus_tag="PLJ64_12590"
     CDS             1688..>2148
                     /locus_tag="PLJ64_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HNC94367.1"
                     /translation="MSETVAEALATTSTGSQPALTEPFEIGGVEISNRVLLAPLASIG
                     NWFVRMQARRYGAGLAVSEMVSSFGLHYGNEKTMKELLRIHPDEHPVSIQLFGQSPEA
                     MESGARIAAEAGADLIDINMGCPVPKVRKTGAGAELLSDPELALDLARAAGA"
BASE COUNT          359 a          665 c          754 g          370 t
ORIGIN      
        1 cgaagttcca ctttgtgccg gtatgcggca caaagtgggc actcgacgat cgggagccgg
       61 ggccggggct cagtcggaac gcgggccgag gccgggggtc agtcggaaac gggagccggg
      121 gccggggctc agtcggaaac gggggcggcg gcgttttcgc ggagcggggc gagccactcg
      181 gtcggcaggc ccggggggac gtcacccgcg tagaccggag cggtgcggac tgcggcctcg
      241 tagatcgggc ggttttcgag gaaggggcgg aggtcctgaa gggtggccgc caggcggatg
      301 ctggtggcga tctggaaatc ggcggcgttc ggggtggcac cgccgagcgt gccgtcggcc
      361 acccaggcgt ccaggcggtc gagccactcc ggcagcgcga tcaggtttgc cttcacgttc
      421 tcgtcggttg attcgttcac cttgtactcg ccccagatga tcgggccgga agtgtaggcg
      481 gcgaccttgg gcggcagggg caggctcgaa ccttcgagga aggtggcgat cgatttcggc
      541 tgccgacgga ccgcattcca gaggatctgg cggaccgcat gctggagttc ggcctcgccg
      601 aaggcctcgg ccatcagcac ctgcttgcgg gcgtcctcgt cggcgggcag cagcggcggc
      661 tccggcacga tccggtcgag cgcccgcgcg atgtcaaccg aaccctgaag cttctcgccg
      721 ttgaacttga gggcgggaac cgtggtcccg gggaagccgg cggcacgcac cacggccttg
      781 tgcatgaggg gcatcaggtc gatcgtcttg aagtcgatgc ccttgtaatc gagcatcagc
      841 ttcacgctga ccgcgggatg ggaaccggga agtgagaaca gcgtcgcctt gctcatgggg
      901 aggatcataa gtcttgacat ttgccacact cccttgttca tgcttctggc aattgacgtc
      961 ggcaacaccc agacccatct cggagccttc gagggcaacg aactgacccg tcactggcgt
     1021 tttcagacga gggccggggc gaccagcgac gagctggccg aacggatatc cggcctgatg
     1081 gaactgcggg gtaccggctt cgacgacctc gacggactgg tcgtttcctc ggtcgtgccg
     1141 ccgctcggct ccgagtacac caggctggcc cagcaatacc tcgatgtggg ttgtctcctg
     1201 gtcggaccgg gtatccgcac cggaatgtca attgaaatcg acaaccccgc cgaagtcggt
     1261 gcggaccggc tggtcaacgc gatcgccgcc tacgacgagg tgggcgatgt ctgcgtgggg
     1321 gttgatttcg gcaccggcat caatttcgac gcggtctctg ctgacggtgc ctacctcggt
     1381 ggcgcgatcg cccccggtgt cgaaatctcg ctgaccgcgc tgaccgaacg ggcggcgcgg
     1441 atttcccgga tcgacctcgg tgaaccgaag aaggcgatcg gcacgtcctc gcgcgaggcg
     1501 atccaggtcg gggtgcttta cggcttcgcc gggctggtcg acggcatcgc ccgccgcatc
     1561 aaccgtgaac tgggcggtga cgcccgcttc atcgcgactg gcggttacgc caagtcgatc
     1621 gtcccctttt cggaagagat cgacgagata gacgagctcc tgacgctcaa ggggctgagg
     1681 atcatccatg agcgaaaccg tggctgaggc cctcgccacc acctccaccg gatcacagcc
     1741 cgcactcact gaacctttcg agatcggcgg ggtggagatc tccaaccggg tgctgctcgc
     1801 gccgctggcc agcatcggca actggttcgt ccgcatgcag gccaggcgct acggggccgg
     1861 cctcgccgtt tcggaaatgg tttccagttt cggccttcac tacggcaacg agaagacgat
     1921 gaaggagctg ctccggatcc accccgacga gcacccggtt tcgattcagc tattcggcca
     1981 gtcgccggag gcgatggagt ccggggcgag gatcgccgcc gaagccgggg ccgacctgat
     2041 cgacatcaac atgggttgcc cggtgccgaa ggtccgcaag accggggccg gggccgaact
     2101 gctttccgac cccgagttgg cccttgatct ggctcgggca gccggggc
//
LOCUS       DAOQKN010000290         2139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_40713_length_2139_cov_2.921305, whole genome shotgun sequence.
ACCESSION   DAOQKN010000290 DAOQKN010000000
VERSION     DAOQKN010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40713_length_2139_cov_2.921305"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2139
                     /locus_tag="PLJ64_12595"
     CDS             <1..>2139
                     /locus_tag="PLJ64_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94368.1"
                     /translation="GVYFYSSDQLVAADTSGDTDLYLRSGGETTLLSADGKGRSGAGG
                     ADFVERFDDGRKIAIVTGDRWTADDRDDGQDLYIRAGDQLTLITKGPAKKRSSYRYNE
                     MFNRYLGGDRYVVSTDSPLVAEDSDRNEDVYLVDLDDPASPWLVSESPLQITSGPIGK
                     VQSEPPFSFSFRQVGARPAFECSIDLDPFRPCRSPLVLRNVKPGGHKVRVRSTGPSGQ
                     GSRVAASRRFIYRPQGTNYEIPEISRISADSGGLLYTIWSDRRISGWGSEYRIFRRPS
                     ANSNSSLIAGGDSYLVGADRDGTNTVIRTRRPLSEADDDQITDLYLRTPQETELIPDG
                     LNLIDPPILGGISADGRTLLFATREQLVPADTDQATDAYVTGPEGIHLMSPGDGIGFD
                     VWPFDISADGSAVTFQTSEALAPEDSDGGKSLYWSDGDTVRFVTSGGLKKACSDCDLD
                     YSDPDPVWFAPDGSRIVLDTAKNLLPADIDRKLDVYEWTPAGLELASAAEEPLITFDA
                     SVPQIGSHLREPRDVAPWGDTNAAVAPDGSMLISTREKLTTEDQDMSTDIYLRSEGRL
                     KLLDPSSKLNEGMRALGASIDLRSVIVVTKSRLVPGDRDDVADIYRLKDGKAARISRS
                     EPEAFRGNTQFVWISDDGREVAFTTNYPFDRADRDEGARDLYIWKQGKRSLVTTRTGE
                     LGKLSVNFVKSGGSGQFFYLTTRRSMLPADQ"
BASE COUNT          430 a          669 c          662 g          378 t
ORIGIN      
        1 ggcgtctact tctattcatc ggatcagttg gtcgctgccg atacgtcagg cgatacggac
       61 ctctaccttc ggtcgggtgg cgagaccacg cttctgtcgg ccgacggcaa gggcagaagc
      121 ggggccggcg gagcagactt cgtggagcgc ttcgatgacg gccgaaagat cgcgatcgtc
      181 accggcgacc gctggaccgc ggatgaccgg gacgacggcc aggacctcta catccgcgca
      241 ggcgaccaac tgaccctgat caccaagggc ccggccaaga agaggtccag ctatcgctac
      301 aacgagatgt tcaaccgtta cctgggtggc gatcgctatg tggtttcgac ggacagcccc
      361 ctggtcgccg aggacagtga ccgcaatgaa gacgtgtacc tggtggatct ggacgatccg
      421 gcgtcaccct ggctcgtttc cgagagcccg cttcagatca cttcggggcc gatcggcaag
      481 gtccagagcg aacctccctt ctccttcagc tttcgtcagg tgggcgcccg gcccgcgttc
      541 gaatgctcga tcgacctgga tcccttccgc ccctgcaggt cgccactcgt actccgtaac
      601 gtcaagccgg gtggccacaa ggtcagggtg cgctcaaccg ggcccagcgg gcagggttcg
      661 cgagtcgcgg cttcgcggcg tttcatttac aggccgcagg gaaccaacta cgaaataccg
      721 gaaatctcga ggatctcggc agatagcggc ggtctgctct acaccatctg gtccgaccgc
      781 cggatttccg gttggggcag cgagtaccgg atcttccggc ggccctcggc gaattcgaat
      841 tcgtcactga tcgccggggg agattcgtat ctggtcgggg ctgaccgtga cggaaccaac
      901 acagtgatcc ggacccgccg accgctgtcc gaggccgatg acgaccagat caccgacctg
      961 tacctccgga ccccgcagga aaccgagctg attccggacg ggctgaatct gatcgatccg
     1021 ccaatcctgg gcgggatcag cgccgacggc cgaaccctgc tgttcgcaac ccgggaacag
     1081 ctggttccag ccgacacgga tcaggcaacc gatgcctatg tgaccggacc ggaaggaata
     1141 catctgatgt cgcctggcga cggcatcgga ttcgatgtct ggcccttcga tatctcggcc
     1201 gacggatccg ccgtgacctt ccagacctcg gaggctcttg ccccggagga ttccgacggt
     1261 ggcaagagcc tttactggag tgacggcgac acggtccggt tcgtgaccag cggaggcctg
     1321 aagaaggcct gctctgactg cgatctggac tacagcgacc ccgatccggt ctggtttgcc
     1381 cctgacggtt cccgcattgt tcttgacacc gcaaagaacc tgcttccagc cgacatcgac
     1441 cggaaactcg acgtgtacga atggaccccg gccggcctgg agctggccag cgccgccgaa
     1501 gaaccactga tcaccttcga tgcttcggtg ccgcagatcg gaagccacct gcgcgaaccg
     1561 cgggatgtcg caccgtgggg tgacaccaac gcggcggtag caccagacgg ctcaatgctg
     1621 atttcaacgc gggagaaact gaccacggag gaccaggaca tgagcacgga tatttacctg
     1681 cgttcggaag gccgattgaa actgcttgat ccttccagca agctgaacga aggaatgcgc
     1741 gcgctcggag ccagcattga cctgcgatcg gtgattgtgg tcaccaagtc acgactcgtg
     1801 ccgggtgacc gggacgatgt ggccgatatc taccggctca aagatggcaa agcggccaga
     1861 atctcaagga gcgaaccgga agcgttccgg ggcaataccc agttcgtttg gatctccgat
     1921 gacggcaggg aagtggcgtt cacaaccaat tacccgttcg accgggctga ccgggacgaa
     1981 ggcgcacggg atctctacat ctggaagcag ggcaagagga gcctggtcac cacccgcacc
     2041 ggcgagctgg gcaaactgtc ggtcaacttt gtcaagtccg gtggtagcgg ccagttcttc
     2101 tacctgacga cccgtcgcag catgctcccc gctgaccag
//
LOCUS       DAOQKN010000291         2106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_41948_length_2106_cov_4.163335, whole genome shotgun sequence.
ACCESSION   DAOQKN010000291 DAOQKN010000000
VERSION     DAOQKN010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41948_length_2106_cov_4.163335"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="PLJ64_12600"
     CDS             complement(<1..430)
                     /locus_tag="PLJ64_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94369.1"
                     /translation="MKHLKNTLAFLLAATVSLAFASSAGAAGESVTATTSISGMGGKL
                     FKEKQVAARLNIHAEVTTPDTSPQVLPMKNVKVTFPAGMAIRPNNSKTPVCSDSKLSN
                     TSNLSNPSGVVASCSRSVVGSGTAAIYLAKVNLPTALITDP"
     gene            complement(625..1494)
                     /locus_tag="PLJ64_12605"
     CDS             complement(625..1494)
                     /locus_tag="PLJ64_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94370.1"
                     /translation="MTFKPNNSKTPACTDSKLSITSNLADPAGVVASCPKSVVGSGTA
                     AIFLAKVNLPETLIVDPILIVFNAGTTGSGQPKLKIYGYSKTTNVGILMTGTLKGSVL
                     DIAIPVLSNDSAVKYFQLNIPGGQLDREDINVHVHGLDPNYVQAKCASSPLKTNAVFE
                     LGERSYPSGTPTGPTTTVTSPQTTQDCVGKAGTPKLGFNKVNGPSAVKNGKTGSYKVT
                     IKNPGTATVKNVVVTNNRGGKTKAGNIAPGKTKTVSVKAKIKGKKGKKVAVKFPAKAG
                     NVKASTTKQVKVK"
     gene            complement(1890..>2106)
                     /locus_tag="PLJ64_12610"
     CDS             complement(1890..>2106)
                     /locus_tag="PLJ64_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94371.1"
                     /translation="NGTATARDIKVTVSGSGKGQARAANIAPGKSLTIPVKVKVTGPK
                     GKPAKLAFRISGQGTRTRAGFRGRIAG"
BASE COUNT          335 a          585 c          719 g          467 t
ORIGIN      
        1 tggggtcggt gatgagcgct gtcggcaggt tgaccttggc gaggtagatc gccgcggtac
       61 cgctgccgac gaccgacctg ctgcaggagg cgacgacgcc cgaaggattc gaaaggttcg
      121 aagtgttgct gagcttgctg tccgagcaga cgggagtctt cgaattgttc gggcggattg
      181 ccattcctgc ggggaacgtg accttcacgt tcttcatcgg caggacctgc ggcgaggtgt
      241 ccggtgtcgt cacctccgca tgaatgttga gcctggctgc aacctgcttc tccttgaaaa
      301 gcttgccccc catgccggag atgctggtgg ttgcggtgac cgattcaccc gcggcgccgg
      361 cgctggaagc gaaggcgagc gagaccgtgg cggccagaag gaaagcgagt gtgttcttca
      421 ggtgtttcat catttcctcg aatgtcgtgg acggagtgaa actcaggcgg ccgcgggagc
      481 cgctacctga agaaaccccc agctcacccc gcaagctttg gcgtttaccg gacctttacc
      541 gggcggggac agggaccggg ccgggaactg cacgaaaaag ggccgcccgg aggcggccct
      601 ttttcgctgt tgattgaggc agtcttactt gaccttcacc tgcttggtgg tgctggcctt
      661 gacgttgccg gccttggccg ggaacttgac ggcaaccttc ttgcccttct tgcccttgat
      721 cttggccttg acggaaaccg tcttggtctt gccgggggcg atgttgccgg ccttggtctt
      781 gccgccgcgg ttgttggtaa caacaacatt cttgacggtg gcagtgcccg ggttcttgat
      841 ggtcaccttg taggaaccgg tcttgccgtt cttgacggcg ctcggaccgt tgaccttgtt
      901 gaagccgagc ttcggggtac cggccttgcc gacgcagtcc tgggtggtct gcggcgaggt
      961 gacggtggtc gtcgggccgg tcggggtgcc gctcgggtag gaacgctcgc cgagctcgaa
     1021 gacggcgttg gtcttcagcg ggctcgaggc gcacttggcc tgaacgtagt tcgggtcgag
     1081 accgtggacg tgaacgttga tgtcctcacg gtcgagctgg ccacccggga tgttcagctg
     1141 gaagtacttc acggcggagt cgttgctcag caccgggatg gcaatgtcca ggacgctgcc
     1201 cttcagggtg ccggtcatca ggatgccgac gttggtggtc ttcgagtagc cgtagatctt
     1261 cagcttcggc tggccgctgc cggtggtgcc ggcgttgaac acgatcagga tcgggtcaac
     1321 gatcagggtt tccggcaggt tgaccttggc caggaaaatc gcggcggtac cggaaccaac
     1381 caccgacttg gggcaggaag cgacgacgcc cgcgggatcg gccaggttcg aggtgatgct
     1441 cagcttgctg tcggtgcagg ccggcgtctt cgagttgttc ggcttgaagg tcatgccgtt
     1501 cgggaacggt gatcttcacg ttcttcatcg ggttgacctt cggagaatcg gacggagtgt
     1561 ttacttcggc tcgaatcgtg agctgggaag acgaagtgac ttccttgaag tagttgccgc
     1621 cgccctgagg ggcgagggtg gtggtcgcgg tcacggtctc gttcgccgcg ttggcattcg
     1681 ctaccagcgc gaagggaacg atcatggcga tcacgagggc tatgtatttc ttcatgggat
     1741 tcattggtgg gcctcaatca ggtgggttgg agagaacgag acgggccgcc gaaatttgcg
     1801 gtccgtcaca agtaaatacc ctcgccagac ggctttgttt cgccgtttac ccaaccttaa
     1861 ttgcgaaccc gccaggcggc ttcggggggt cagccggcga ttcggccccg gaacccggcc
     1921 cgggtcctgg tgccctggcc ggagatccgg aaagcgagct tcgccggttt gcctttcgga
     1981 ccggtcacct tgaccttgac cggaatcgtc agtgacttgc cgggagcgat gttcgcggcc
     2041 cgggcctggc ccttgccgct tcccgaaacg gtcaccttta tgtctctggc agttgcggtt
     2101 ccgttg
//
LOCUS       DAOQKN010000292         2091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_42504_length_2091_cov_5.032417, whole genome shotgun sequence.
ACCESSION   DAOQKN010000292 DAOQKN010000000
VERSION     DAOQKN010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42504_length_2091_cov_5.032417"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="PLJ64_12615"
     CDS             <1..745
                     /locus_tag="PLJ64_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC94372.1"
                     /translation="PAQLSGGQQQRVGVARALAADPPVMLMDEPFGAIDPINRARLQN
                     EFLRLQADLKKTVLFVTHDVDEAIKMGDRVAVLKKGGHLAQYATPTELLMEPADDFVE
                     DFVGADRALKRLALMRVRDVNLWTAPLAQAGDPTEPVKTAVAAAEVPYALLVDGENRP
                     QGWLSDRDLAGERVPETPDSAADPLIDADSVLRDGLANLLQSGSLYAPVVDARGRLTG
                     VLSVEIVSDFLASDEAAESELTAAERPHD"
     gene            738..1484
                     /locus_tag="PLJ64_12620"
     CDS             738..1484
                     /locus_tag="PLJ64_12620"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC94373.1"
                     /translation="MIEAAAGPPLMRFAAIDQSFFTDRSGGSCIQDNKLFCPGWVADN
                     YDRYVDPTLQHLVLVGSAVAAGFLIAFSLAILAHRHRFLQTPFLAGTGILYTIPSISF
                     FFLLLPITGRGALTAIIALTAFNLQIIFRNITTGLRNVPANVTEAATGIGMTRSQVLW
                     QVEIPLAMPEIIAGLRIATVSTVALATLAVFANGGGLGTEIVTGSNITFKTGIIVAGS
                     IAVIMAIVFDGVLLLTGKLLAPWKRKRMAV"
     gene            1484..>2091
                     /locus_tag="PLJ64_12625"
     CDS             1484..>2091
                     /locus_tag="PLJ64_12625"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNC94374.1"
                     /translation="MPGPVFALPAGFLSLFGEAFNFVFSGREAETGGVNVGGTGQVID
                     LATKQLEVTLLALAVAILLAMPVGLWLGHRQKGEFLAVSAGNAGRAIPELALIALMAA
                     AIGVGLVNLVVALAILGIPPILTNTYVGISQVDRASVEAARGMGMTETGIVRKVELPL
                     AVPTIMGGVRTAAINIIATATIAPLAGILTLGDFIIARNVYG"
BASE COUNT          324 a          748 c          668 g          351 t
ORIGIN      
        1 cccggcgcag ctctccgggg gccagcagca gcgggtcggc gtcgcgcgcg ccctggcggc
       61 tgacccgccg gtgatgctga tggacgaacc gttcggggcg atcgacccga tcaaccgcgc
      121 ccggctccag aacgaattcc tgcgccttca ggcagacctc aagaagaccg tcctcttcgt
      181 gacccatgac gtcgacgagg cgatcaagat gggcgaccgg gtcgccgttc tgaagaaggg
      241 cggccacctg gcccagtacg caacgccgac cgagctcttg atggaaccgg ccgatgattt
      301 cgtcgaggat ttcgtcggtg ccgaccgggc cctcaaacgg ctggccctga tgcgggtgcg
      361 tgacgtgaac ctttggaccg cgcccctcgc ccaggccggc gacccgaccg agccggtgaa
      421 gaccgcggtg gccgcggccg aggttcccta cgcgctgctg gtcgacggcg agaaccgccc
      481 gcagggatgg ctctcggacc gggacctggc cggtgaacgg gtgccggaaa ccccggattc
      541 ggccgccgac ccgctgatcg acgccgactc ggtactccgt gacggcctcg ccaacctgct
      601 ccagagcggc tcgctctacg ctccggtggt cgatgcccgc ggccggctga ccggggtgct
      661 ctcggtcgag atcgtctccg acttcctcgc ctccgatgaa gctgcggaat ccgagctgac
      721 cgcggccgag cggccccatg attgaggcag cggcaggccc gccgctgatg cgcttcgcgg
      781 cgatcgatca gagcttcttc accgaccgct ccggcggcag ctgcattcag gacaacaagc
      841 tcttctgccc gggctgggtc gccgacaact acgaccgcta cgtcgacccg accctgcagc
      901 acctggtcct cgtcgggtcc gcggtcgcgg ccgggttcct gatcgccttc agcctggcga
      961 tcctcgctca ccggcaccgc ttcctccaga ccccgtttct ggccggcacc ggaatcctct
     1021 acacgatccc ctcgatctcc ttcttcttcc tgctgctgcc gatcaccggc cgcggcgcgt
     1081 tgaccgcgat catcgccctg accgcgttca acctccagat catcttccgc aacatcacta
     1141 ccggcctgcg gaacgtgccg gccaacgtga ccgaagcggc gaccgggatc ggaatgacca
     1201 gatcccaggt gctctggcag gtcgagatcc ccctggcgat gccggagatc atcgccggcc
     1261 tgcggatcgc aacggtcagc acggtcgccc tggcgacgct ggccgttttt gccaacggag
     1321 gcggtctcgg gaccgagatc gtcaccggct cgaacatcac cttcaagacc ggcatcatcg
     1381 tcgccggctc gatcgcggtg atcatggcaa tcgtctttga cggcgtcctg ctcctgaccg
     1441 gcaagctcct cgccccctgg aagcggaaac ggatggcggt ctgatgccgg ggcccgtctt
     1501 cgccctgccc gccgggttcc tcagcctctt cggcgaagca ttcaactttg tgttctccgg
     1561 ccgggaggcc gagaccggcg gcgtgaacgt tggcggcacc ggacaggtaa tcgacctcgc
     1621 gaccaagcag ctcgaagtga ccctgcttgc cctggctgtc gcgatcctgc tggcgatgcc
     1681 ggtcggactg tggctcggtc accgccagaa gggcgagttc ctcgcggtat ctgccggcaa
     1741 cgcgggccgg gcaattcccg agctcgcgct gatcgccctg atggcggccg cgatcggggt
     1801 ggggctggtc aacctggttg tcgccctggc gatcctagga atcccgccga tcctgaccaa
     1861 cacctacgtc gggatcagcc aggtcgaccg ggcctcggtc gaggccgcac gcgggatggg
     1921 catgaccgaa accgggatcg tgcgcaaggt cgagttgccg ctcgcggtgc cgaccatcat
     1981 gggcggggta aggaccgcgg cgatcaacat catcgccacc gccaccatcg ccccgctggc
     2041 cgggatcctg acccttggtg acttcatcat cgcccgcaac gtctacggcg a
//
LOCUS       DAOQKN010000293         2085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_42692_length_2085_cov_3.878325, whole genome shotgun sequence.
ACCESSION   DAOQKN010000293 DAOQKN010000000
VERSION     DAOQKN010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42692_length_2085_cov_3.878325"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="PLJ64_12630"
     CDS             complement(<1..485)
                     /locus_tag="PLJ64_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC94375.1"
                     /translation="MSGPDLSSPRAKNLALMVLALTQFIVVIDASITNVALPSIGKAL
                     DIDEKSLSWVVNSYTLVFGGFLLLGGRLADFLGRRRMFMIGMTLFGAASLAGGFAQTE
                     AWLIIARAVQGLGAAIASPAALSIVTTTFAEGSERNRALAIWGAVAGAGGAAGVLLGG
                     VL"
     gene            588..1163
                     /locus_tag="PLJ64_12635"
     CDS             588..1163
                     /locus_tag="PLJ64_12635"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC94376.1"
                     /translation="MTSAEKTEKTLRADARRNREKIFAAARELFARDAEGVQMEEIAS
                     HAGVGVGTIYRHFPTKQALLTEMVRTRFSRFTEIALEAERIDDPREAVETLIRQSCVE
                     VEGDAGFQLAMMSPDELEWEGIEQEKAAFAEPATRIIDRAVESGAVRSDFSFADFPML
                     MCGITSTMYFAPAGSDWQRHLDLILAGLRPG"
     gene            1189..>2085
                     /locus_tag="PLJ64_12640"
     CDS             1189..>2085
                     /locus_tag="PLJ64_12640"
                     /inference="COORDINATES: protein motif:HMM:NF024918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HNC94377.1"
                     /translation="MRIVYGVNGEGMGHATRSELVISDLLRDHEVRVVASGAAYRYLS
                     GIFEDVSQVFGPSFAMDQGRIQRWASFTGTLSSARRQLPANVREWISTIDKWKPEVAV
                     SDFEPLTGNYARLSGTPLVCVDNIHMIDRCRHDREITAGAVEDVQLARAVTRAMVPHA
                     GDYVIPTFFYPELARGRTSLVPSILRNAIIEAEPVRGEHLVVYSGGGKDLIDTLRDAP
                     LPVHLYGMRDGDEVGTTDGAIEYRPRSIEGFLEDLVSSRGVITGGGFSLLSEAVYLGK
                     PTLAMPLAGQFEQLMNARYLERE"
BASE COUNT          373 a          679 c          697 g          336 t
ORIGIN      
        1 agcacgccgc cgagcagaac cccggctgcg ccgccggcac cggccaccgc gccccagatc
       61 gccagggccc ggtttcgttc agagccttcg gcgaaggtgg tggtgacgat cgagagtgct
      121 gcgggtgaag cgattgcggc gccaagaccc tggaccgccc gggcgatgat cagccaggcc
      181 tcggtctggg caaaaccacc ggccagtgaa gcagcgccga acagggtcat gccgatcatg
      241 aacatccgcc ggcgcccgag gaagtcggcg aggcgaccgc cgagcagcag gaaaccgccg
      301 aagacgaggg tgtagctgtt gaccacccag gacaggctct tttcgtcgat atcgagggcc
      361 ttgccgatcg agggcagggc gacattggtg atcgaggcat cgatcaccac gatgaactgg
      421 gtgagggcaa gcaccattag cgcaaggttc ttcgcgcgag ggctgctgag gtcaggtccg
      481 gacaagaggt gctcctggga ttcgctggtc ggtttgaaat cggaagcgga attcctgttc
      541 cacttattgc ggtactatac ggaaaccaga ttccgtttgt caacccgatg acttccgccg
      601 agaaaacaga gaaaacgctg agggctgacg cccgtcgcaa ccgcgagaag atcttcgccg
      661 ccgcccgcga gctgtttgcc cgcgacgccg agggggtgca gatggaggag atcgccagcc
      721 acgccggggt cggggtcggc acgatctacc ggcacttccc gaccaagcag gcgctcctga
      781 ccgagatggt ccggaccagg ttctcgcgct tcaccgagat cgccctcgag gccgagcgga
      841 tcgatgaccc ccgcgaggcg gtcgagaccc tgatccgcca gtcctgcgtg gaagtcgaag
      901 gcgacgccgg cttccagctc gcgatgatgt cccccgacga gctcgagtgg gaagggatcg
      961 agcaggagaa ggcggccttc gccgagccgg ccacccggat catcgaccgg gccgtcgaaa
     1021 gcggcgccgt ccgcagcgac ttctcgttcg ccgatttccc gatgctgatg tgcggcatca
     1081 cctcgaccat gtacttcgcc ccggccgggt ccgactggca gcgccacctg gacctgatcc
     1141 tggccggact tcgcccgggc tgagcgcgcc gccgggtagg ctggcggaat gaggatcgtt
     1201 tacggggtaa acggcgaggg catgggtcac gcgacccgct ccgagctcgt catctccgac
     1261 ctgctccggg accatgaggt ccgcgtcgtt gcctccggag cggcgtatcg gtacctgagc
     1321 gggatcttcg aggatgtgag ccaggtcttc ggcccctcgt tcgcgatgga ccagggaagg
     1381 atccagcgct gggcttcctt caccggcact ctctcttccg cccgcaggca gctgccggcc
     1441 aacgtgaggg aatggatctc gaccatcgac aagtggaagc ccgaagtcgc ggtcagtgac
     1501 ttcgagccgc tgaccggcaa ctacgcccgg ctttccggca ccccgctggt ctgcgtcgac
     1561 aacattcaca tgatcgaccg ttgccgccac gaccgcgaga taacggcggg ggccgtcgag
     1621 gacgtccagc tcgccagggc ggtgacccgg gccatggttc cgcatgccgg tgactacgtg
     1681 attccgacct tcttctaccc ggagttggca cgaggcagaa ccagcctggt cccctcgatc
     1741 ctgcgcaacg cgatcatcga agcggaaccg gtccgcggcg agcatctggt cgtctattcc
     1801 ggcggcggca aggacctgat cgacaccctg cgggatgcgc cactgccggt tcacctctac
     1861 gggatgcgcg acggcgacga ggtcgggacc accgacggcg cgatcgagta ccggccgcgc
     1921 tcgatcgagg gtttcctcga ggatctggtc agctcccgcg gcgtgatcac cggcggtggc
     1981 ttctcgctgc tgtccgaggc ggtctacctc ggcaagccga ccctggcgat gccgctcgcg
     2041 ggccagttcg aacagctgat gaacgcgcga tacctggagc gcgag
//
LOCUS       DAOQKN010000294         2064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_43461_length_2064_cov_5.555500, whole genome shotgun sequence.
ACCESSION   DAOQKN010000294 DAOQKN010000000
VERSION     DAOQKN010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43461_length_2064_cov_5.555500"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2064
                     /locus_tag="PLJ64_12645"
     CDS             <1..>2064
                     /locus_tag="PLJ64_12645"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HNC94378.1"
                     /translation="YRDNYRYFFPLNTNPPANGYNNENTTLTTPSYSVHSIDRWIDDQ
                     LRITSGSASGADILDRDKVAFTPGYCGRTEDTFTGRENFNSSDNAEGAFVTNRAGPVR
                     AIRSYIGANSGPYTQREQIFYRDRQDSRTYLRVHSIPYVMSFTDYSPAASGMTWRSSI
                     NTTGVTVDGTPDLFTVPGFTDPRDLTGIQYPSAGAGVSGWEQITGTQGTVDAVSRLVT
                     DIPNVAVTAYYLDNSSPSTTFNQSQMTGDPTYQCTGDAFAYGASGLQLKVATPIGTDL
                     PNTDPRAGGSENEVTMERSIFYGPPGGNSVEAESRRQQVDNPIELTSRRHIRPPDGPP
                     DASPASRDFGTLEISESPGASQQFTIHNAGNYELSLSQAVVSGGAGFEIDGDSCSNQI
                     LPSGMSCSIGIRFDPASAGAVTANLVIPNQSAYPDGNTVHPDISISLAGTGAADPSLN
                     ITPSSHDFGQSAVNLSTPSQVFTVSNPARSSRYVDLVARVGSGAAHFLNTSGNCVART
                     LGPGASCTYRLAFKPTSPGLKNAVLEVRGSGGGSLASASVQGTGISVAVTGTTGQTGQ
                     TGPIGPTGNTGQTGPTGETTATGPTGPTGSTGETTTGPTGPTGPIEPTGPTGPTEPVG
                     PTGPTGPTEPTGPTGPTQPTGQTGPTGPVRPSTACLKSRRNLAAARKRLATAKKSLKK
                     AKSQP"
BASE COUNT          417 a          742 c          620 g          285 t
ORIGIN      
        1 gctacaggga caactaccgc tacttcttcc cgctcaacac caacccgccg gccaacggct
       61 acaacaacga gaacacgacc ctcaccaccc cgagctactc ggtccattcg atcgaccgct
      121 ggatcgatga ccagctcaga atcacctccg gctcggccag cggggcggac attctcgatc
      181 gcgacaaggt cgccttcacc cccggctact gtggtcgcac cgaagacacc ttcaccggcc
      241 gtgagaactt caacagcagc gacaacgccg aaggcgcctt cgtcaccaac cgtgccggcc
      301 cggtccgggc gatccgttca tacatcggcg ccaacagcgg cccgtacacg cagcgtgaac
      361 agatcttcta ccgcgatcgc caggattccc ggacctatct gcgggttcac tcgatcccgt
      421 atgtcatgtc gttcaccgac tactcgccgg ctgcatccgg gatgacctgg cgttcatcga
      481 tcaatacgac cggggtgacc gtggacggca cgcccgatct gttcaccgtg cccggattca
      541 ccgacccccg ggatctgacc ggcatccagt acccgtccgc gggcgcggga gtgagcggct
      601 gggaacaaat caccggcacg cagggaacgg tcgacgcagt ctcgcggctg gtcaccgata
      661 tcccgaatgt cgcggtcacc gcctattacc tcgacaacag cagccccagc accactttca
      721 accagagcca gatgaccggg gatccgacct atcagtgcac aggggatgcg ttcgcctacg
      781 gcgcaagcgg gcttcaactc aaggtcgcga ccccgatcgg gaccgacctg cccaacaccg
      841 acccgagagc gggtggcagt gagaacgaag tgaccatgga gcgctcgatc ttctacggac
      901 cgcccggtgg caattccgtc gaggcagaga gcaggagaca gcaagtcgac aatccgatcg
      961 aactgacttc caggcgccac atccggcccc cggacggacc acccgatgcg tcgccggcga
     1021 gccgggactt cggcaccctg gagatcagcg aaagcccggg cgcgagccag cagttcacga
     1081 tccacaacgc cggcaattac gagctgtcgc tgagtcaggc cgtggtcagt ggcggagccg
     1141 ggttcgaaat cgacggtgac agctgttcga accagatcct gccgtccgga atgagttgca
     1201 gcatcgggat acggttcgac ccggcttcgg caggtgcggt caccgccaac ctggtgattc
     1261 cgaaccagtc cgcgtatccc gacggaaaca cggttcaccc cgacatctcg atctccctcg
     1321 ccgggaccgg cgcggccgac ccgtcgctga acatcacgcc gtcctcgcat gatttcgggc
     1381 agtccgcggt gaatctctcg actccaagtc aggtgttcac ggtcagcaac ccggcccgct
     1441 cgtcgcgata tgtggacctt gtggccaggg tcggcagcgg cgccgcacat ttcctcaaca
     1501 ccagcggcaa ctgtgtggcg aggacactcg ggccgggtgc gtcctgcact taccgcctgg
     1561 ccttcaagcc gaccagcccg gggttgaaaa acgcggttct ggaggtgcgt ggatccggtg
     1621 gcgggtccct ggccagcgcc tccgttcagg gcaccggaat ctccgtcgct gtgaccggaa
     1681 ccaccggcca gaccggtcag accgggccga tcgggccgac cgggaacacg ggccagaccg
     1741 gcccgaccgg ggagaccacg gcgaccggac cgaccgggcc gaccggaagc accggcgaga
     1801 caaccaccgg acccaccgga ccgaccggac cgatcgaacc gaccggaccc acgggcccaa
     1861 ccgaacctgt tgggcccacc gggccgaccg gcccaaccga accgaccgga cccacggggc
     1921 cgactcagcc cacgggccag accgggccga ccggaccggt caggccgtcg accgcctgcc
     1981 tcaaatccag gcggaacctc gccgccgccc gcaagcggct ggccacggcg aagaagtcgc
     2041 tgaagaaggc gaagagccag ccag
//
LOCUS       DAOQKN010000295         2061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_43582_length_2061_cov_6.184447, whole genome shotgun sequence.
ACCESSION   DAOQKN010000295 DAOQKN010000000
VERSION     DAOQKN010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43582_length_2061_cov_6.184447"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PLJ64_12650"
     CDS             complement(<1..336)
                     /locus_tag="PLJ64_12650"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNC94379.1"
                     /translation="MKAIQIQEFGGPEVMNLVELPDPVPAEGEVLVEINRAGVNFSDT
                     HITNDQYVARQQLPLIPGIEFVGVAEGGRRVAAVVPNGSYAEKIAVPESSLVEIPDEI
                     DDEQAAGIMI"
     gene            375..1286
                     /locus_tag="PLJ64_12655"
     CDS             375..1286
                     /locus_tag="PLJ64_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNC94380.1"
                     /translation="MSLTVVGSIAFDAVATPFGERERMLGGSAVHFSLAASFFTDVRV
                     VGPVGDDFTETELAVLENRGVITDDIERVSGETFFWKGHYEYDLNTAHTDDTRLNVFG
                     DFEPKLSDTSKAADLLFLANIQPDLQRQVRAQCPEAGFVGLDTMNLWIDTAKESLEAA
                     ISEVDCLLINDAEIRQLTGEPNLARAAAAVMEMGPKAVIAKQGEYGAALFTPGGFFAL
                     PGFPLEDVRDPTGAGDSFAGGFLGYLDGEAGGIDQQTLRTAMGYGTVLASYNVEEFGT
                     ERVSRLSRDEIESRLEALRSMTSFSLT"
     gene            1342..>2061
                     /locus_tag="PLJ64_12660"
     CDS             1342..>2061
                     /locus_tag="PLJ64_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HNC94381.1"
                     /translation="MSTEQQTEAPAEGDGGGVAAGNDTLSVTDNRTGRTYELPIEDDT
                     IKALDLRQIKVHEDDFGMMAFDPAFTNTASCRSSITYIDGDAGILEHRGIPIDQLCEH
                     STFLEVAYLLVFGELPTRPQLEAWVHDVTHHTFVHEDIKKFLTGFRYDAHPMGMLLAS
                     TGALSTFYPDSNQISDPVERYLATIRLIAKMPTLAAFSYRHNMGLPYVYPDNDLSYPE
                     NFLSMMFKMTEMKYKPNPVLER"
BASE COUNT          383 a          702 c          596 g          380 t
ORIGIN      
        1 gatcatgatt ccggcggcct gctcgtcatc gatttcatcg gggatctcca ccagagaact
       61 ttccggcacc gcgatcttct cggcgtagct gccgttcggg accaccgccg caacccgacg
      121 gccaccctcg gccacgccca cgaactcgat tcccgggatc agcggcagct gttgcctcgc
      181 cacgtactgg tcgttggtga tgtgagtgtc ggagaagttg actcccgccc ggttgatttc
      241 gaccaggact tccccttcgg ccggcaccgg atcgggcagt tcgaccaggt tcatcacctc
      301 cggcccgccg aactcctgaa tctggatcgc tttcacaaag ccatcttttc acggtggcgg
      361 atacgatccc gcccatgtct ctgaccgttg tcgggtcgat cgcctttgat gcggtcgcca
      421 caccatttgg cgagcgcgag cgcatgctcg gcggctccgc agtccacttc tcgcttgctg
      481 cgagtttctt caccgatgtc cgcgtggtcg ggccggtcgg ggatgacttc accgagaccg
      541 aactggcagt gctcgaaaac cgcggcgtga tcaccgatga catcgaacgg gtcagcggcg
      601 agaccttctt ctggaagggt cactacgagt acgacctgaa cacggcccac accgatgaca
      661 cccgcctcaa cgtcttcggc gatttcgagc cgaagctctc ggacacctcg aaggcggccg
      721 acctgctctt tctggccaac atccagccgg acctgcagcg ccaggtccgg gcccagtgcc
      781 cggaggccgg tttcgtcggg ctcgacacga tgaacctctg gatcgacacc gcgaaggagt
      841 cgctggaagc ggcgatttcc gaagttgact gcctgctgat caacgacgcc gagatccgcc
      901 agctgaccgg cgagccgaac ctcgcccggg ccgctgccgc ggtgatggag atgggcccga
      961 aggccgtgat cgccaagcag ggggagtacg gcgccgccct gttcacgccc ggcggtttct
     1021 ttgccctgcc cgggtttccc ctggaggacg tgcgcgaccc gaccggcgcc ggcgattcgt
     1081 tcgccggcgg cttcctcggt tacctcgacg gcgaggccgg tggcatcgac cagcagactc
     1141 tgcgcaccgc gatgggctac ggcaccgtgc tcgcctccta caacgtcgag gagttcggca
     1201 ccgaaagagt cagccgcctg agccgcgacg agatcgagtc gcgcctcgaa gcgctgcggt
     1261 cgatgaccag tttctcgctg acttaaccga cctgagatac tggtccgaag aaagatcaga
     1321 gaacaggatc cgaggagaag catgtcgacc gaacagcaga ccgaagcgcc cgccgagggc
     1381 gatgggggcg gagtagccgc cggaaacgac accctctcgg tcaccgacaa ccggaccggt
     1441 cgcacctacg agcttccgat cgaggacgac acgatcaagg cgctggatct gcgccagatc
     1501 aaggtccacg aagacgactt cgggatgatg gccttcgatc cggccttcac caatacggcc
     1561 tcctgccgct cctcgatcac ctacatcgac ggtgatgccg gaatcctcga gcaccgcggt
     1621 atcccgatcg accagctttg tgagcactcg accttcctcg aagtggctta cctgctggtc
     1681 ttcggcgagc ttcccacacg cccgcagctc gaagcctggg tccatgacgt cacccatcac
     1741 accttcgttc acgaggacat caagaagttc ctcaccggct tccgctacga cgcccacccg
     1801 atgggcatgc tgctcgcctc gaccggtgcg ctttcgacct tctaccctga ttcgaatcag
     1861 atcagtgacc cggtcgagcg ctacctggcg acgatccgcc tgatcgccaa gatgccgact
     1921 ttggccgctt tctcctaccg gcacaacatg ggtctgcctt acgtctatcc ggacaacgac
     1981 ctgtcgtacc cggagaactt cctctcgatg atgttcaaga tgaccgagat gaagtacaaa
     2041 cccaaccccg tcctggagcg g
//
LOCUS       DAOQKN010000296         2049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_44020_length_2049_cov_5.249749, whole genome shotgun sequence.
ACCESSION   DAOQKN010000296 DAOQKN010000000
VERSION     DAOQKN010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44020_length_2049_cov_5.249749"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="PLJ64_12665"
     CDS             complement(<1..468)
                     /locus_tag="PLJ64_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94382.1"
                     /translation="MRGAPTRRARRQNITPSGLMLVVFGFMALTFIVLLPAKAGAAYL
                     DGAGTASLQNSPVEPEAPAPDSGASGSAVSSGGGLGVPGLPEISRVICLRQCVSSSRA
                     TRGAIIAIRGENLDRVIRVIFRGKRGWLRAKYRTRSKAAVRVTVPRQAIRGYLA"
     gene            706..>2049
                     /locus_tag="PLJ64_12670"
     CDS             706..>2049
                     /locus_tag="PLJ64_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HNC94383.1"
                     /translation="MTAIRQKNVTAAQWNANGSAVESTDLTKEENVVFGSNVFSIKTQ
                     KERLPADVFDRLQGTLERGEALDVDLADAVADAMKDWALENGATHYTHVFQPLTGLTA
                     EKHDSFFRPTDEGGSIAKFKGSELIQGEPDASSFPTGGIRATFEARGYTAWDPTSPAF
                     LLDNPNGTLLCIPTAFASWTGEALDAKIPLLRSMDALSRSAVKALKLLGDEETSRVVT
                     TVGPEQEYFLIDEQYFYARPDLVLTGRTLFGAQPAKGHELDDHYFGAIPERVLAYMMD
                     CEAELAKLGVPITTRHNEVAPAQYEVAPMFENSNVGSDHQQLLMQVLQNKARKYGLVC
                     LLHEKPFAGVNGSGKHNNWSMGTDTGHNLLDPGDTPAENLSFLFFCAAVIQAVNKHQG
                     LMRATVANIGQDHRLGANEAPPAIISIFLGGELEKVFETMAAGEGDPNTPDEVLDL"
BASE COUNT          399 a          699 c          611 g          340 t
ORIGIN      
        1 ggcgaggtag ccccggatcg cctgcctggg aacggtcact cgaaccgcgg ccttgcttcg
       61 ggtccgatac ttcgcacgca gccagccccg cttgccacgg aagatcacgc ggattacccg
      121 gtctaggttc tcgccccgaa tcgcgatgat cgcgccccgg gtggccctgc tgcttgaaac
      181 gcattggcgc aggcagatca cccggctgat ctccgggagt ccgggaacgc ccaggccgcc
      241 gcccgatgaa acggccgaac ccgacgcgcc ggaatcaggg gccggtgcct ccggctcaac
      301 cggcgaattc tgaagagatg cggtcccggc tccgtccagg taggcggccc cggctttggc
      361 cggcagcaga acaatgaaag tgagtgccat gaacccgaag acgaccagca tcagaccgct
      421 gggggtgatg ttctgtctcc tggccctgcg cgttggagct cctcgcatcg tggacaccat
      481 aacatgagtt ctagacgatg attccgtgag ctgctgcaca caaacttgca gatcggactt
      541 tcaggtaccc cgtttcatca gtgtcggagc caagcctgtc ccacgggagg gcggccctga
      601 actaaccttg tcctgaggta caagcgaccg catggccgct cggggatagc ctcgacttcg
      661 ctttttccct cgaaatcaat cttgccacca ggaggaactc tccaaatgac cgcgatccgc
      721 cagaagaacg taaccgctgc ccagtggaac gccaacggtt cggccgttga gtccacggac
      781 ctgaccaagg aagaaaacgt cgtattcggc agcaacgtct tctcgatcaa gacccagaag
      841 gagcggctcc ccgccgatgt cttcgaccgg cttcagggca cgctcgaaag gggcgaggcc
      901 cttgacgtcg atctggccga cgcggtcgcc gacgcgatga aggactgggc gctcgaaaac
      961 ggcgccaccc actacacgca tgtcttccag ccgctgacag gtctgaccgc ggaaaagcac
     1021 gattcgttct tccgcccgac cgatgaaggc ggctcgatag ccaagttcaa gggttcggaa
     1081 ctgatccagg gcgaaccgga cgcctcctcc ttccccaccg gcggcatccg cgccaccttc
     1141 gaagcccgcg gctacaccgc ctgggatccg accagcccgg ccttcctgct cgacaacccg
     1201 aacggaacgc tgctctgcat cccgaccgct ttcgcttcct ggaccggcga agccctggac
     1261 gcgaagatcc cgctgcttcg ttcgatggat gccctttcgc gttcggcggt caaggcgctg
     1321 aagctgctcg gtgatgaaga gacttcgcgc gtggtcacga cggtaggccc cgagcaggag
     1381 tacttcctga tcgacgagca gtacttctac gcccgccccg acctcgtcct gaccggccgc
     1441 accctgttcg gcgcccagcc ggccaagggc cacgagctcg acgaccacta cttcggcgcg
     1501 atcccggagc gggtgctcgc ctacatgatg gactgcgagg ccgaactggc caagctcggc
     1561 gtgccgatta ccacccgcca caacgaggtc gccccggccc agtacgaagt ggcgccgatg
     1621 ttcgagaact cgaatgtcgg ttcggaccac cagcagctgc tgatgcaggt gcttcagaac
     1681 aaggcccgca agtacggcct ggtctgcctg cttcatgaaa agcccttcgc cggcgtgaac
     1741 ggctccggca agcacaacaa ctggtcgatg ggcaccgaca ccggccacaa cctgctcgac
     1801 cccggagaca ccccggccga gaacctcagt ttcctgttct tctgcgccgc ggtgatccag
     1861 gcggtcaaca agcatcaggg tctgatgcgg gcgacggtcg cgaacatcgg ccaggaccac
     1921 cggctcggcg ccaacgaggc accgccggcg atcatctcga tcttcctcgg cggcgagctg
     1981 gagaaggtct tcgagacgat ggccgccggc gaaggtgatc cgaacacccc ggatgaggtt
     2041 ctcgacctc
//
LOCUS       DAOQKN010000297         2047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_44108_length_2047_cov_7.918675, whole genome shotgun sequence.
ACCESSION   DAOQKN010000297 DAOQKN010000000
VERSION     DAOQKN010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44108_length_2047_cov_7.918675"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(191..1195)
                     /locus_tag="PLJ64_12675"
     CDS             complement(191..1195)
                     /locus_tag="PLJ64_12675"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HNC94384.1"
                     /translation="MDARRRVSPAELNRKLGQVVKSGHGVITGDDLREIGVSRQTIRR
                     RKESGLLIPVLRDVFTLPGCRPDEIGRRKAAVHAGGSSCCLSHRSALAHHGLIYDQLP
                     VHVLRTGGADRRNGSALKRSRDFGFSVLSHQTRNLPPDEITIIEGIRVATPERALLDF
                     AATGRGSDIGKVLTQGERKGNLCWGRLESILENSRGRRGVTTLREEMDLFDPESVNSE
                     SDPEEDFLRMIRRRSLPMPLVNVWLAPYRVDFLWWHLMLIVELDPYGTHRGKRSFHGD
                     RRKSVELETRGFRVIRLTEKDLYAFEERTARDLETIMEAQAALLGVSVWPAGQNAATR
                     "
     gene            complement(1454..>2047)
                     /locus_tag="PLJ64_12680"
     CDS             complement(1454..>2047)
                     /locus_tag="PLJ64_12680"
                     /inference="COORDINATES: protein motif:HMM:NF014609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HNC94385.1"
                     /translation="LQQVEVSRAGAADPSPEGGEAAAGSGVWRASVLQEPASFGEAVP
                     RDRAARLLNLDESRLHPDYSAQVVSTGFPTVLLPLRNVEALADAVPDRAEFESFEFGD
                     LAVNVYPFVFDVPTRSVRARCFALDLGDFEDPATGSAAGPLVAYLQDILDVDRVEIHQ
                     GVEIGRPSLIEARMDSGRPRVSGRVHVVMTGTATLPG"
BASE COUNT          370 a          674 c          665 g          338 t
ORIGIN      
        1 cggccacact cacctcgtgc cggcgtcctg acacgaggca ggcgccagtt gcgacgctcc
       61 cgtcgtggcg gcactcgcct cgtgtcggca cccacgccgc ggccacactc acctcgtgcc
      121 ggcgtcctga cacgaggcag gcgccagtcg cggggctcgc gtcgtgtcgg gactcgcctc
      181 gtgtcggggc tcacctcgtg gcggcattct gtcccgccgg ccagaccgag acgccgagta
      241 gcgctgcctg ggcctccatg atggtctcga ggtccctcgc ggttctttcc tcgaacgcgt
      301 agagatcctt ctcggtcagc ctgatgaccc tgaagccccg ggtttcgagt tcgacactct
      361 ttcgacgatc gccgtggaat gacctcttgc cccgatgcgt tccatacgga tcgagttcga
      421 cgatcagcat caagtgccac caaaggaagt cgacccggta tggggccagc cagacattca
      481 ccaggggcat cggaagggac cgccgccgga tcattcgaag aaagtcttct tccgggtcgg
      541 actcggagtt gactgattcg ggatcgaaaa ggtccatttc ttcccgcagc gtggtcactc
      601 cccggcggcc tcgagagttc tcaaggatcg actcgagcct gccccagcac aggtttccct
      661 tgcgctcccc ttgggtcagg acctttccta tgtccgatcc cctgccagtc gcggcaaaat
      721 cgagcagtgc acgctccgga gtcgcgaccc ggattccctc gatgatcgtg atctcgtctg
      781 gcggaagatt gcgcgtctga tgactgagga cggaaaaccc gaaatcccga cttcgcttga
      841 gggctgaacc atttctgcgg tccgcgccac cggtccggag cacatggacg ggaagttggt
      901 cgtagatcag gccgtgatgg gcgagagcgc tccgatgcga aaggcagcag ctactcccgc
      961 ctgcgtgaac cgcggccttt cggcgcccga tttcgtcagg ccggcagccg ggcagggtga
     1021 acacatcgcg aaggacgggt atcaaaagcc cggactcctt tctgcgacga atggtctgcc
     1081 tggagactcc gatttccctc aggtcatcac cggtgatcac cccgtgacct gacttgacga
     1141 cctggccaag cttcctgttc agttcggccg ggctgaccct tcgtcgtgca tccatgggaa
     1201 aagcatgaag cggaaaagct cacacatggc tcgacaggag caacaattca gtaacaggaa
     1261 accgccgaag cccgggcggt tgccgaagcc cggggaaccg ccgaagcctg ggcaatcgct
     1321 gaagcctggg caatcgccga agcctgaagc ccggagccgg cggctggtgc cacgagaagg
     1381 gtgccattcc cggggcctgg ttcgtggcag aaccagctct gaggcgaaaa ccccggggac
     1441 cggtgagagc ggttcagccg gggagggtcg cggtgccggt catgaccacg tggacccggc
     1501 cgcttacccg ggggcggccc gaatccatgc gggcctcgat caggctgggg cggccgatct
     1561 cgactccctg gtggatctcg acccggtcga cgtcgaggat gtcctgcagg taggcgacca
     1621 gcgggccggc ggccgaaccg gttgccgggt cttcgaaatc gccgaggtcg agggcgaaac
     1681 agcgcgcgcg gaccgaacgg gtcgggacgt cgaagacgaa gggatacacg ttcaccgcga
     1741 gatcaccgaa ttcgaaactc tcgaactcgg cccggtcggg aacggcatcg gccagcgcct
     1801 cgacgttccg aagcgggagc agcaccgtgg gaaaaccggt cgagacgacc tgggcggagt
     1861 agtccgggtg aaggcgcgac tcgtccagat tgagcaggcg cgccgcccgg tcgcggggaa
     1921 cggcctcgcc gaaactggcc ggctcctgaa ggaccgaggc ccgccagacc cccgacccgg
     1981 ctgccgcctc cccgccctcg gggcttggat ccgcagcacc tgcccggctg acctccacct
     2041 gctggag
//
LOCUS       DAOQKN010000298         2001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_45992_length_2001_cov_5.710175, whole genome shotgun sequence.
ACCESSION   DAOQKN010000298 DAOQKN010000000
VERSION     DAOQKN010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45992_length_2001_cov_5.710175"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="PLJ64_12685"
     CDS             complement(<1..710)
                     /locus_tag="PLJ64_12685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HNC94386.1"
                     /translation="MSDPNSKRPERILVTGGTGLIGSTLCNALIDRGDSVVGLSRDPE
                     RASRSLPRVEWHGWEGERERPPRDAFEGVDAVINLAGEPINQRWNDEVKDRIARSRIK
                     ATKNLVAKIAALDQRPKTLISASAIGYYGDRGDELLYEDAEPGDDFLADIVVRWEEAA
                     RSAEDHGLRVATIRSGHVLDPRGGLLKELMLPFKAGVGGPIAGGKQYMSWVHRHDEIG
                     ILLWALENESVSGPVNATA"
     gene            783..>2001
                     /locus_tag="PLJ64_12690"
     CDS             783..>2001
                     /locus_tag="PLJ64_12690"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HNC94387.1"
                     /translation="MRLVTSNRSRTWTVAAATAALALFGILSGPGPAGAAPARCFGQK
                     VDRVITGSNKTVNVGAREVVFVEGNRITVKATAFSRICAGDGSQTIEAGQGWSFTDGG
                     PGNDRISAAKGDSTVFGGPGDDQIETEKGNNIEVQGGPGDDEIRPGKGNNVKVDGDEG
                     NDRIFLFTQTAKGVVDGGLGNDRIEGSQSHDKLIGGPEKNPRNLPDTDLIIGEGGNDS
                     IFDYGGSGNRLIGLEGADELSSLGDAVSELHGGNGSDKLFSNGGRTAAGVTEQLFGEQ
                     GNDRLYADRPGSNGPAYLDGGEGDDWMYGTSKADTIIANSGIKKIQGEGGDDLLISTA
                     RGAGNFSGGEGRDTISFAAHTPTDRTGPNGGVTVDLGSGNASGASDQNLSGIENVIGS
                     AFDDQIEGAPGVDN"
BASE COUNT          375 a          642 c          668 g          316 t
ORIGIN      
        1 gccgtcgcgt tgaccggtcc gctgaccgac tcgttttcaa gggcccagag caggatcccg
       61 atctcgtcgt gccggtggac ccaggacatg tactgcttgc cgccggcgat cggcccgcca
      121 accccggcct tgaacggcag catcagttcc ttcagcaacc cgccccgggg gtcgagcacg
      181 tggccggagc ggatcgtcgc cacccgcagg ccgtgatcct cggccgagcg ggccgcttct
      241 tcccagcgaa ccacgatgtc cgccaggaaa tcgtccccgg gctcggcatc ttcatagaga
      301 agctcgtcgc cacggtcgcc gtagtagccg atcgcagaag cggagatcag ggtcttcggc
      361 cgctggtcga gggcggcgat cttggccacg aggttcttgg tcgccttgat gcggctgcgg
      421 gcgatccggt cctttacctc gtcgttccag cgctggttta tcggctcccc ggcgaggttg
      481 atcacggcgt cgacaccttc aaaagcatct ctgggaggcc tttcgcgttc gccttcccag
      541 ccgtgccact caacccgggg gaggctgcgg gaagccctct ccgggtcacg gctcaggcca
      601 accacggaat cgccgcggtc gatcagggcg ttgcaaaggg ttgagccgat caggccggtc
      661 ccaccagtga cgagaatgcg ctcggggcgc ttggaattcg ggtcagacat cctgaactag
      721 tctagagttc cgggtaacgg ggcatcgaat gcctcgtcgc ggtgttagca aggggaacag
      781 ggatgagatt ggtcacgtcc aaccggagtc gcacctggac cgttgcagcg gccacggctg
      841 cccttgccct tttcgggatc ctttcgggtc ccgggcccgc cggggcggcc ccggccagat
      901 gcttcggcca gaaggtcgac cgcgtgatca ccggttccaa caagaccgtc aacgtcgggg
      961 cccgggaagt cgtgttcgtc gaaggcaacc ggatcaccgt caaggccacg gcgttcagcc
     1021 ggatctgcgc cggggacgga tcccagacga tcgaggcggg gcagggatgg tcattcaccg
     1081 acggtggacc gggcaacgac aggatttccg ctgccaaggg cgattcgacg gttttcggcg
     1141 gaccgggcga cgatcagatc gagaccgaaa agggcaacaa catcgaggtc cagggtggcc
     1201 ccggcgatga cgagatccgt cccggcaagg gaaacaacgt caaggtcgac ggtgacgaag
     1261 gcaacgaccg gatcttccta ttcacccaga ccgccaaagg cgtcgtcgac ggtggtctcg
     1321 gcaatgaccg gatcgaaggc tcccagagcc acgacaagct gatcggcggc cccgaaaaga
     1381 acccgcggaa cctgcctgac accgacctga tcattggcga gggtggaaac gactcgattt
     1441 tcgattacgg cggcagcggc aaccggctga tcggcctcga gggcgccgac gagctttcca
     1501 gcctcggcga tgcggtttcc gaacttcatg gtggcaacgg gtccgacaag ctgttctcca
     1561 acggtggccg gaccgccgcc ggggtcacgg aacagctgtt cggcgagcag ggcaacgacc
     1621 ggctttacgc ggaccgtccc ggcagcaacg gaccggccta cctcgacggc ggcgaaggtg
     1681 acgactggat gtacgggacc tcgaaggccg acacgatcat cgccaactcg gggatcaaga
     1741 agatccaggg agaaggtggc gacgacctgc tgatctcgac cgcgcgcggg gccgggaact
     1801 tcagcggcgg cgaaggccgg gacacgatct catttgccgc ccacaccccg accgaccgga
     1861 ccgggccgaa cggcggcgtg acggtcgatc tcggctcggg caacgcttcc ggcgcctcgg
     1921 accagaacct gagcggcatt gaaaacgtga tcggctccgc tttcgatgac cagatcgagg
     1981 gcgcgccggg cgtcgacaac g
//
LOCUS       DAOQKN010000299         1943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_48617_length_1943_cov_4.953919, whole genome shotgun sequence.
ACCESSION   DAOQKN010000299 DAOQKN010000000
VERSION     DAOQKN010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48617_length_1943_cov_4.953919"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..342
                     /locus_tag="PLJ64_12695"
     CDS             139..342
                     /locus_tag="PLJ64_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94388.1"
                     /translation="MDFKKLGQQAGKLKKQAEDKLGDRAEPENLKRDAKELREILAGD
                     GSLAEKAKQAKKEVVDRDKPVGN"
     gene            complement(442..999)
                     /locus_tag="PLJ64_12700"
     CDS             complement(442..999)
                     /locus_tag="PLJ64_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94389.1"
                     /translation="MESRISSWLRCYRCWSRNLEVQVHYDAIRPIDPETGIPTEGDDE
                     IQESVVQCLDCMHDQPHLTFERDRVVPIEDRWERMVAGTPWVASCTVTVEADQVEACA
                     GPEAVESLTFGAFGDAGTREFFTHVRFHKHHEDQIAVHLLVELYARNPEEASEVLTGA
                     ARGTIEITSLAEESRPPAHASGDTH"
     gene            complement(1055..1834)
                     /locus_tag="PLJ64_12705"
     CDS             complement(1055..1834)
                     /locus_tag="PLJ64_12705"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNC94390.1"
                     /translation="MATKSQQKRKELRDKRMQAEQAAQSADKRQNLAKILGIAAFLVV
                     VGVVVAVVAISSSGGGDEPKGSGDIDKLLAGIPQNGTTLGEANSKVTLYEFGDLQCPI
                     CEQYSEDATPDIINNIVRPGKAKWNFKQWAILGPDSTLAAKAALAAAEQNRYWEFIEN
                     FYANQGIENSGYVTDDFLTEIANDAGVPDIDKWNADRESAAIAKQVEQTDAQASSLGF
                     SGTPSFGVQVGNGDIEPVQTTSSTAAAGEIAKAIEKAQSGN"
BASE COUNT          343 a          592 c          633 g          375 t
ORIGIN      
        1 cggcgaaaag tgccgccgcg ggcagcaaaa tgatgaacaa catgctgacc attccgacca
       61 gaaagctggc gcctgccgcg aaccagagga agacccggaa tccattcacc ggacagaggc
      121 tatcggtagt tttgtctcat ggacttcaag aaacttggtc agcaggccgg gaagttgaag
      181 aaacaggctg aggacaagct gggggaccgg gccgagcccg agaacctgaa gcgggacgcc
      241 aaggagctac gcgagatcct cgccggcgac ggctcactcg ccgaaaaggc aaaacaggca
      301 aagaaagaag tcgtagaccg ggacaagccg gtgggcaatt aacccgatgc gccgccggac
      361 ggagcccctc ccaacaatta gatcgtatga gtgggagggg cgggttactg gtggagggcg
      421 ggtcggaccc cgcccgactt cttaatgggt gtcgcccgag gcgtgggcgg ggggccggga
      481 ctcctcggcc agtgaggtga tctcgatcgt tccccgggcg gcgccggtca gcacttcgct
      541 cgcctcctcc gggttgcggg cgtacagctc gaccagcagg tgtaccgcga tctggtcctc
      601 gtggtgcttg tggaagcgga cgtgggtgaa gaactcacgg gttccggcat cgccgaaggc
      661 gccgaaggtc agcgattcaa ccgcttccgg tcccgcgcag gcctcgacct gatcggcctc
      721 gaccgtgacc gtgcatgagg cgacccaggg cgtcccggcc accatgcgtt cccagcgatc
      781 ctcgatcggt accacccggt cccgctcgaa agtcaggtgc ggctggtcat gcatgcagtc
      841 caggcactgg accaccgatt cctggatctc gtcatcgccc tcggtcggga taccggtctc
      901 cgggtcgatc ggccggatcg cgtcgtagtg gacctgaacc tcgaggttgc gtgaccagca
      961 ccggtagcac ctgagccatg aagagatacg ggactccatg ctcatatctt tgcgcctcgg
     1021 agacctcggc ggggcatgtg gctcgctatg tgggtcagtt acccgactgc gccttctcga
     1081 tcgccttggc gatctcgccg gctgccgcag ttgaactggt ggtctggacc ggctcgatgt
     1141 caccgttgcc gacctggacc ccaaacgacg gggtgccgct gaagccgagg ctcgaggcct
     1201 gcgcgtcggt ctgctcgacc tgcttggcga tcgcggcgga ctcccgatcg gcattccact
     1261 tgtcgatgtc cggaacgccg gcgtcgttgg cgatttcggt gaggaagtcg tcggtcacat
     1321 aaccggaatt ctcgatgccc tgattggcgt agaagttctc gatgaattcc cagtaacggt
     1381 tctgttccgc agcggcgagc gcagccttgg cagcgagggt cgaatccggg ccgagaatcg
     1441 cccactgctt gaagttccac ttggccttgc cgggccggac gatgttgttg atgatgtccg
     1501 gggtggcgtc ctccgagtac tgctcgcaga tcgggcactg caggtcaccg aactcgtaca
     1561 gcgtcacctt gctgtttgct tcgccgagcg tggtgccgtt ctgcgggatt ccggccagca
     1621 gcttgtcgat gtctcccgat cccttcggct cgtcgccgcc tccgctggag ctgatcgcga
     1681 cgaccgccac gaccacgccg accacgacca gaaaggccgc gatcccgagg atctttgcga
     1741 ggttctgacg cttgtcggcg ctctgcgcag cctgctcggc ctgcatgcgc ttgtcgcgga
     1801 gttcttttct cttctgctgc gatttggtgg ccattggcgg ggatgctact aagaggcggg
     1861 atctggctct gcctcgtcag caaatgaggt ttgctgctcc ggatctccgg ttccgtagta
     1921 accgatcaga cggcaggaat tga
//
LOCUS       DAOQKN010000300         1919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_49752_length_1919_cov_4.432940, whole genome shotgun sequence.
ACCESSION   DAOQKN010000300 DAOQKN010000000
VERSION     DAOQKN010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49752_length_1919_cov_4.432940"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1448)
                     /locus_tag="PLJ64_12710"
     CDS             complement(<1..1448)
                     /locus_tag="PLJ64_12710"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNC94391.1"
                     /translation="MTESLADGFEPVSFEQWSEAATRGDENLALMTLLENGVEAKWLY
                     TPEDAIAPDPAGLPGQAPFTRGTRAGRHWQIRQEQTQPDRGRANAEILEDLNGGVTEI
                     TLRFDRAAREGLAPGTDGFSAARGIDGIAISNLDQLGEVLDGVYLDMAGVALEAGAAA
                     PSAAALLAAHWRENGIAPDSAIGSFRYDPLGEIARTGELPCSPEDALAAAGGLAAETA
                     RDWPKARALAVDTGIYVEAGATAGWELGIAVATGVEYLRAAAAAGLDPAEAASQIEFT
                     LAVGPDQFLEMAKFRAIRRLWARVLEESGVAAEARFSPTYARTSARMISAVDPWVNML
                     RVTTAAFAAGTGGADGVTVTPFDRMIGQPGDLGRRIARNTQIILQDESSLGRIADPLA
                     GSWYGEELTDEVAKAGWERFREIEREGGALAALRSGLIATTLGGLADEREDDLNHRRR
                     VMTGINEFPLLGDDGTEPERADLSRVEGDSAR"
     gene            1531..>1919
                     /locus_tag="PLJ64_12715"
     CDS             1531..>1919
                     /locus_tag="PLJ64_12715"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HNC94392.1"
                     /translation="MKRERKQPPPLPIAPARFRLIRVKQDPLKAILQRFALASLLLLI
                     VTFITYFGREGYVDNEHPNQLLSFVDSLYYATVTITTTGYGDIVPVTEASRLVTTIVV
                     TPIRVLFIVLLVGTTLQVLAEQSRFQFR"
BASE COUNT          281 a          659 c          652 g          327 t
ORIGIN      
        1 aatcgcgccg aatcaccttc caccctcgaa aggtcggccc gctccggctc ggtgccgtca
       61 tcgccgagca gcgggaactc gttgatcccg gtcatcaccc ggcggcgatg gttgaggtca
      121 tcctcgcgct cgtcggcgag cccgcccagg gtggtggcga tcaatccgga acggagcgcg
      181 gccagggcgc cgccctcgcg ttcgatttcg cggaaccgct cccagccggc tttggccacc
      241 tcgtcggtca gctcttcccc gtaccaggag ccggccagcg ggtcggcgat gcggccgagt
      301 gaggactcgt cctgaaggat gatctgggtg ttgcgggcga tccggcggcc gaggtcgccc
      361 ggctggccga tcatccggtc gaagggagtg acggtcaccc cgtcggcgcc accggtgccg
      421 gccgcaaagg cggccgtggt aacgcgaagc atgttgaccc aggggtcgac ggcgctgatc
      481 atccgggccg aagtcctggc gtaggtcggg gagaaccggg cctcggcagc gacgccggat
      541 tcctcgagca cgcgggccca gagccttcgg atcgcgcgga acttggccat ctcgaggaac
      601 tggtcgggtc cgaccgcgag ggtgaactcg atctgcgacg cagcttcggc gggatcaagg
      661 ccggcggcgg ccgccgcccg caggtactcg actccggtcg ccacggcgat cccgagctcc
      721 cagccagcgg tggctcccgc ctccacatag atgccggtgt ctaccgcaag cgcccgggcc
      781 ttcggccagt cgcgcgcggt ctctgccgcc agccctccgg ccgcggcaag cgcatcctcg
      841 ggcgagcagg gcagctcgcc ggtgcgggcg atctcgccga gcgggtcgta gcggaaggag
      901 ccgatcgccg agtcgggggc gatgccgttc tcgcgccagt gggcggccag cagcgccgcc
      961 gccgaagggg cagcggcccc ggcttcaagg gcaaccccgg ccatgtcgag gtacacgccg
     1021 tcgagaacct cgccgagctg gtcgaggttg gaaatcgcga tgccgtcaat gccgcgggcc
     1081 gcgctgaatc cgtcggttcc cggagccagg ccctcgcggg cggcgcggtc aaaccgcaga
     1141 gtgatctcgg tgacgccgcc gttcaggtcc tcgaggattt ccgcgtttgc gcggccccgg
     1201 tcgggttggg tctgctcctg gcggatctgc cagtgccttc cggcccgggt cccgcgggtg
     1261 aatggagcct ggccgggcag accggccgga tcgggggcga tcgcatcctc cggggtgtag
     1321 agccacttcg cttcgacccc gttctcgagc agcgtcatca gggcgaggtt ctcgtcgccc
     1381 ctggtggccg cttcgctcca ttgctcgaag gaaacgggtt caaagccgtc ggcgagggac
     1441 tcagtcatgg cactccagag tggtaaacac gggtctttca gggtacctgt ccggagcggc
     1501 tttcggtctt gccatctatg ctgctgccac ttgaaacggg agcgcaaaca gccaccgccg
     1561 ctgccgatcg ccccggcccg gtttcgcctg atccgggtca agcaggatcc gttgaaggcg
     1621 atcctccagc gcttcgccct ggccagcctg ctgcttttga tcgtcacctt cattacctac
     1681 ttcggccggg aggggtacgt cgacaacgag cacccgaacc agctgctgag tttcgtcgac
     1741 tcgctctact acgcgacggt gacgatcacc accaccgggt acggcgacat cgtgccggtc
     1801 accgaggcat cccgcctggt caccacgatc gtggtcaccc cgatccgcgt tctcttcatc
     1861 gttttgctgg ttggcactac gcttcaggtc ttggccgagc agtccagatt ccagtttcg
//
LOCUS       DAOQKN010000301         1918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_49813_length_1918_cov_3.829844, whole genome shotgun sequence.
ACCESSION   DAOQKN010000301 DAOQKN010000000
VERSION     DAOQKN010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49813_length_1918_cov_3.829844"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="PLJ64_12720"
     CDS             complement(<1..247)
                     /locus_tag="PLJ64_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94393.1"
                     /translation="MSEKDAAPAIDFPTAKNTSIEQLAAMASPTDIQFAPSGKVVEPG
                     NNRYGFAAFDSQGQQQTDLDVALYATSGPKSKVLGPFP"
     regulatory      complement(876..1023)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            complement(1294..>1918)
                     /locus_tag="PLJ64_12725"
     CDS             complement(1294..>1918)
                     /locus_tag="PLJ64_12725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNC94394.1"
                     /translation="EASSAVEGLRDRAGISEVVMLTGDSEPVAAAIAKRSGITRWRAG
                     LLPEDKLDEVIRLEREVGPTAMVGDGVNDSPALAGSTVGVAMGAAGSDVALDSADVAL
                     MGDDLMRLPDAINHSRWAVRIMKQNVSVSLVTKAIFVVLAPLGFVSLIAAIAVDMGVS
                     LLVTLNGLRLLRRPATHSLRETPGDPDPDAVATRAEGDACEEDCCSP"
BASE COUNT          349 a          611 c          583 g          375 t
ORIGIN      
        1 ccgggaaggg ccctagaact ttcgacttcg gtccgctcgt cgcatacagc gcgacatcga
       61 gatccgtctg ttgttgtccc tgcgagtcga atgccgcgaa cccatagcga ttatttcctg
      121 gctcgaccac ctttcccgaa ggtgcgaact gaatgtcggt cggtgacgcc atggcggcga
      181 gctgttcgat cgaggtgttc ttggccgttg gaaagtcaat ggcaggagca gcatccttct
      241 cgctcatctc gctgttcgaa tcgccgcaac cgactagagc cagcacagtc agcgccgcca
      301 gaagaacgag gctccatggt ctgcccgggg ttagcgagct gaaccccact tacggaggta
      361 gggcgtcggg ttgctcacgc ttccaccgcg gtaccagccc ggtcgcgtcc acatctcaaa
      421 atggagatga caggcggtcg agcgtccggt cgacccgacc agtccgattc gctggcccgc
      481 ctttacgagt tggcccgccc ggatttccgg tgtgcgaagc atgtgcatgt acacgtagtc
      541 gaacttcgat gacgtcccgc gaatcacggc atagttgcca gccccgtcag cctggtagtc
      601 gacaagacga actcttccgg tctgagccgc gaccaccggc ctggtgcatt tggcgagaag
      661 atcctggccc tgatgccccc ggcccgctcc tagtccgtcg ccccaggtga ctgaaccacg
      721 gatcgggaac gcgcccttgg ccggagccgg gctcggagaa gccggtgaca gacctcccgt
      781 tgaggcggta gcgatgccgg agaacattcc tagagctcca aaggtcaata acgccctaat
      841 ggccaaccgt ccgatgacgg aatcgtgatt cattccttct ctctttctcg cgccttcggg
      901 gttagctgac gggctcgcgc tgaaagaggt gcgctacgca gagtgtgcga ttcgccccag
      961 agacttgatg gcctcaagtg ggtcccccga tccggaacac tccggattcg gctcattgga
     1021 tttctcacat actacgacac tacgtagtag ttccctttat gggggtcccg ccggccctcc
     1081 ggccgggggg cgcccttccg gattgtgggt gggccggcgg gatccagatc atagcgcttg
     1141 ctacgacagt ctgtagtaga tcccgttgac ccggacaagg atgtccactg ctccgcgtca
     1201 agaggccaga agcggagagt tgctcgttca aagcgtcgat gcccaggcca acgggattcc
     1261 gaccctagca actcctacga cgtcctgtag tagttagggt gaacagcagt cctcttcgca
     1321 tgcgtcgcct tccgcacgag tcgcgaccgc gtctggatct ggatccccgg gggtctctct
     1381 cagggaatgg gtcgctggcc ggcgcagcag acgcagaccg ttcagggtca cgagcagtga
     1441 gacacccatg tcgacggcga ttgcggcgat caggctcacg aacccaaggg gcgccaggac
     1501 cacaaagatc gccttggtga ccaaggagac ggatacgttc tgcttcatga tccggaccgc
     1561 ccatcgtgag tgattgatcg cgtcgggaag tcgcatgaga tcgtcaccca tcagggcgac
     1621 atcggccgag tcgagtgcga cgtcgcttcc ggccgcaccc atcgcgaccc cgacggtcga
     1681 cccggcgagg gcgggtgagt cgttgacccc atcgccgacc attgcggtgg gtccgacctc
     1741 cctctcgaga cgtatgacct cgtcgagttt gtcttcgggt agaagacctg cccgccagcg
     1801 agtgatgccg ctcctcttcg cgatggccgc ggccacgggc tcgctgtcgc cggtgagcat
     1861 tactacctcg ctgattccgg cccggtccct gagtccctcg accgccgacg aggcttcg
//
LOCUS       DAOQKN010000302         1909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_50292_length_1909_cov_4.278857, whole genome shotgun sequence.
ACCESSION   DAOQKN010000302 DAOQKN010000000
VERSION     DAOQKN010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50292_length_1909_cov_4.278857"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="PLJ64_12730"
     CDS             complement(<1..910)
                     /locus_tag="PLJ64_12730"
                     /inference="COORDINATES: protein motif:HMM:NF013044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HNC94395.1"
                     /translation="MKVGSGNGVKGSVSGGLPLSPDDVARALRPLEHASMLPPAAFVD
                     QAVFEWEQKHLFHDGWICLGHVSQVSEQGKFIRREVGPDSVVVIGGEDGRPHAFLNVC
                     RHRGARVIEEADGQVKRRLQCPYHAWSYALDGTLVAAPHMDGVEDFDKSCYGLIPVRM
                     ATIGGLVLVDLGGEAPEPDAHVGELIEHLDRYRTAELVSGGRIDYEVAANWKGIAENY
                     NECLHCPGVHPELNALSNYMSGEEMQGSGSWCGGSMFITAEDAATMGKDGGHAGNRAR
                     IEGLTEEDDKTILYFALFPNALVSMHP"
     gene            1045..>1909
                     /locus_tag="PLJ64_12735"
     CDS             1045..>1909
                     /locus_tag="PLJ64_12735"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNC94396.1"
                     /translation="MNETVTSPDLEGLDEAAIDLRQRARKFVDEVLIPLEQEAELLHG
                     RLPDEAVRMVREKALATGLTGGLHKPEHGGQGWSKVEWFLVEEQLGRSTNAISWHIPN
                     GYNFWDLASGKHVEKWLKPLLRGETRDAYAVTERNAGSDPSGIETRAVRTDGGFRING
                     EKWFVTSGDVADVLVVMANLEEDGELLPTLFVVEKSLGGVETIEDPGFTHSYPDGHPT
                     IRFVDVEVGEDDVIGGIGGGDEVQKAWFAEERLGIAARGIGSMWRLLEETVDWAANRE
                     QGGSRILDYQGV"
BASE COUNT          328 a          622 c          640 g          319 t
ORIGIN      
        1 cgggatgcat cgagaccagc gcgttgggaa agagcgcgaa gtagaggatg gtcttgtcgt
       61 cttcttcggt cagtccctcg attcgcgccc ggttgccggc gtggccaccg tccttgccca
      121 tcgtcgcggc gtcctcggcg gtgatgaaca tcgagccccc gcaccaggaa ccggaaccct
      181 gcatctcttc cccgctcatg taattcgaaa gcgcgttcag ctccgggtgg actccggggc
      241 agtggaggca ttcgttgtag ttctcggcga tgcccttcca gttcgcggcg acctcgtaat
      301 cgatcctccc cccgctgacc agttcggcgg tgcggtagcg gtcgaggtgt tcgatcagct
      361 cgccgacgtg ggcgtccggc tccggtgcct cgccgcccag gtcgaccagg accaggccgc
      421 cgatcgtcgc catccggacc gggatcaggc cgtaacagga cttgtcgaaa tcctcgaccc
      481 cgtccatgtg cggggccgcg accagggttc cgtccagcgc gtaggaccag gcgtggtacg
      541 ggcactggag ccggcgcttg acctgaccgt ctgcttcctc gatcacccgg gcgccgcgat
      601 ggcggcagac gttgaggaac gcgtggggcc ggccgtcctc accgccgatc acgaccaccg
      661 agtcgggccc gacctcgcgg cggatgaact ttccctgctc gcttacctgg gagacatggc
      721 cgaggcagat ccagccgtca tggaagagat gcttttgctc ccactcgaag acggcctgat
      781 cgacgaaggc ggccggcggc agcatcgacg catgttccag gggacgtagc gcccgggcca
      841 cgtcatccgg actcaggggg agcccgccgg agacgctgcc cttgacgccg ttgccgctgc
      901 ccaccttcat ctcgatcctc cggttgtgcc gacgggctcg ggatgggccc gcctagagcg
      961 atactagcgc cggttgacct tgtagtcaat cgcatgtact ctggagacat tccgcttgac
     1021 caaacgagat cggcgaggga gtacatgaac gaaaccgtca caagccccga tctggagggc
     1081 ctcgacgagg cggcgatcga cctccggcaa agagcccgga aattcgtcga cgaggtcctg
     1141 atcccgctcg agcaggaagc ggagcttctt cacggccggc tgcccgacga ggcggttcgg
     1201 atggtcaggg aaaaagccct cgcgaccggt ctcaccggcg gccttcacaa gcccgaacac
     1261 ggcggccagg gctggagcaa ggtcgagtgg ttcctggtcg aggagcagct tggccgttcg
     1321 accaacgcga tctcctggca catccccaac ggttacaact tctgggatct cgcttccggc
     1381 aagcacgtcg agaagtggct gaagccgctc ctgcgcggcg agacgcgcga cgcctacgcg
     1441 gtgaccgaac ggaacgccgg ctcggacccc tcggggatcg agaccagggc ggtccggacc
     1501 gacggcggct tccggatcaa cggagagaag tggttcgtga cttcgggcga cgtggccgac
     1561 gtgctggtcg tcatggccaa cctcgaagag gacggagagc tcctccccac tctcttcgtg
     1621 gtcgagaaat cgctgggcgg agtcgagacg atcgaggacc ccggcttcac ccactcctat
     1681 ccggacggcc acccgaccat ccgcttcgtt gacgtcgagg tcggcgagga cgatgtgatc
     1741 ggcgggatcg gcggcggcga cgaggtccag aaagcctggt tcgccgagga gaggctcggc
     1801 atcgcagctc gcgggatcgg ttcgatgtgg cggctgctcg aggagaccgt cgactgggcc
     1861 gcgaaccgcg agcagggcgg ctcccggatc ctcgactacc agggggttt
//
LOCUS       DAOQKN010000303         1903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_50590_length_1903_cov_4.945887, whole genome shotgun sequence.
ACCESSION   DAOQKN010000303 DAOQKN010000000
VERSION     DAOQKN010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50590_length_1903_cov_4.945887"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /locus_tag="PLJ64_12740"
     CDS             complement(<1..1115)
                     /locus_tag="PLJ64_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94397.1"
                     /translation="MVRGRVLPWTLLPATGFAAIIAVGGIITCFPGLAEFTTGAVTGL
                     AVAGYLALFTGGPRGPARSPAFFKPRSEWVWPAVAAAGVFFVFGAPVILSGSATFAGY
                     VKLDDTSTWLAFTDHVMAHGHSVAGLAPSSYEATVQINLSSGYPLGAFIPLGVGHELT
                     GQDSAWLFQPYLALMASFMSLVFYELAAPVVRDHRLRAAAVFIAAQPSLLVGYAFWGG
                     IKEVATALLVAILAAGAAGLARHPEPRGATVVPIVSGAALIGVMGAGGLPWLAVIFAG
                     AALLMAAKIWSLRREGGEVSGLVRKMLIASAIVLVGVVAIGLPTVFASGTLFSPNQDA
                     LTKGSEEMGNLIRPLGLAQYPGPWPVGDFRVEPVSNLA"
     gene            complement(1184..1783)
                     /locus_tag="PLJ64_12745"
     CDS             complement(1184..1783)
                     /locus_tag="PLJ64_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94398.1"
                     /translation="MSGAPTTVTPEAPKMGARQRAMVLGIAAILVGVAVWGLTACNRD
                     GDPSSASEGVSMANTGDARIVSDDELTGLPEEVGHSLFWAGPKSGTQVEFSSDGTGNV
                     HLRYLTDGAEAGDPNQSYLNIGSYPFADAYEATRKLANGPDVVKVTEGEGTGFYDPNN
                     PYSVIIAWPSEPDLQVEVYDPVKNRALEVVRSGDIVPVS"
BASE COUNT          395 a          619 c          607 g          282 t
ORIGIN      
        1 gccagattcg acaccggctc gacccggaaa tcaccgactg gccaaggtcc agggtactga
       61 gcaaggccga gcggccggat cagattgccc atttcctcgg accccttggt cagcgcatcc
      121 tgattcgggg agaaaagggt tccagaggca aagacggttg gcaaaccgat tgccaccact
      181 ccgaccagga cgatcgcact cgcgatcagc atcttcctga ccagccccga aacctccccc
      241 ccttcccggc gcagactcca gattttcgca gccatgagca gggctgcgcc ggcgaagatt
      301 actgccagcc agggcagacc gccagcgccc atgaccccga tcagcgccgc accggaaacg
      361 atcgggacga cggtggcgcc tcgcggctcc ggatgccgcg ccagtccagc cgcaccggcc
      421 gccagtattg cgactagaag agcagtcgcc acttccttga ttccgcccca gaacgcgtag
      481 ccgaccaaga gcgatggctg agcggcaatg aagaccgcgg cagctcgcag gcggtgatcc
      541 cgaaccaccg gtgcggccaa ctcgtagaag acgagggaca tgaagctggc catcagggcc
      601 agatagggct ggaagagcca ggccgagtcc tggccggtca actcgtgacc gacccccagg
      661 ggaatgaacg cgccgagcgg atagccggat gagagattga tctgaacggt cgcttcgtaa
      721 ctggacgggg ccaagccggc aaccgaatgt ccgtgagcca tcacgtggtc ggtgaaggcg
      781 agccaggtcg aggtgtcgtc cagcttgacg taaccagcaa acgtggccga tcccgagagg
      841 atgaccgggg ccccgaaaac gaagaagacc ccggccgcgg caacggcagg ccagacccat
      901 tcggaccggg gcttgaagaa ggccggactg cgcgccggtc cgcggggacc accggtgaag
      961 agcgccaggt aaccggccac cgccagcccg gtaacggctc cggtggtgaa ctcggccagg
     1021 cccgggaaac aggtgatgat cccgccgacc gcgatgatgg ccgcgaaacc ggtcgccggc
     1081 aggagcgtcc agggcagaac ccggccgcgg accacttcgg ccagcagacc gacaccgacg
     1141 cttagcgcca gcaggacgag agggaagatg acccaggcga gaatcaggac acaggcacga
     1201 tatcgccact gcggacgact tcgagggccc ggttcttgac cgggtcatac acctcgacct
     1261 ggaggtcggg ctcgctcggc caggcgatga tcacgctgta aggattgttc gggtcgtaaa
     1321 atccggttcc ttcaccttcg gtgaccttga ccacatccgg tccgttggcg agcttccggg
     1381 tcgcctcata agcgtcggcg aaggggtagc tgccgatgtt gaggtagctc tggttcgggt
     1441 caccggcttc ggccccgtcg gtcaggtaac ggaggtggac gttgccggtt ccgtcggagc
     1501 tgaactcgac ctgggtgccc gacttcggtc ccgcccagaa caaactgtga cccacctctt
     1561 ccggcaggcc ggtgagttcg tcatcgctga cgatccgggc atcaccggtg ttggccatag
     1621 aaacgccctc cgaggccgac gacgggtcgc cgtcccgatt gcaggcggtc agcccccaga
     1681 ccgcgactcc gaccaggatc gccgcaattc cgagcaccat tgctcgttgg cgggcgccca
     1741 tcttgggcgc ttcaggcgtg actgtcgtcg gggctccgct cacagaagtt gcgaagacta
     1801 gaccagcttc ctttaggaaa cctcaaaagc gcgaggacgc aaatcgagag cccggaagcg
     1861 gattcgaacc gctgacccct tccttaccat ggaagtgctc tac
//
LOCUS       DAOQKN010000304         1897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_50893_length_1897_cov_3.229099, whole genome shotgun sequence.
ACCESSION   DAOQKN010000304 DAOQKN010000000
VERSION     DAOQKN010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50893_length_1897_cov_3.229099"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="PLJ64_12750"
     CDS             complement(<1..959)
                     /locus_tag="PLJ64_12750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNC94399.1"
                     /translation="MSEPSAPRGNPKLSVLDLAPVVEGASPVTALANSTELVRAAERL
                     GYHRHWVAEHHNMTGIASSAPAVIIAHLAANSETIRVGSGGVMLPNHQPLVVAEQFGT
                     LESLNPGRIDLGIGRAPGTDQVTAAALRRGLDPLSDEDLPRQLGELLGFFTGEWPSGH
                     PYQQITAVPGEGAMPEIWLLGSSGYTAQVAGMLGMPYSFAHHFSAGNTEAAVQLYRSR
                     FEPSAFLEEPKVMIGVGVICAETDEEARWLAGPSRLAFARLRSGRPGRFPTPEEAAAH
                     DFTPDEEAVVASVAKGHIFGSPETVQAGLVDLIERTGADELIIS"
     gene            complement(956..>1897)
                     /locus_tag="PLJ64_12755"
     CDS             complement(956..>1897)
                     /locus_tag="PLJ64_12755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94400.1"
                     /translation="IEGPLLGVNLTAYTSDGYSKPSVRKAIKTLADLGGTAITLVPTW
                     YMKSSSANAIEPDEEKSPSDESLAKVISWIRQAGMQVIMKPHVDVIDDSYRGDIQPAD
                     REAWFRSYGTFIDHFASFSTSHSVDLFVAGTELKTMSSETDRWRAVIQTVRDRFTGPI
                     TYAANWDEVDQVQFWDDLDAIGVDAYYPLTRDISKAPSLEELVTAWQGIASQLQAKSA
                     QWERPILLTEVGYPSQVGATVKPYEVTDQPADQNIQALAYEATFKALSGSDWLKGISW
                     WSWRADPSPEENLEIDYPPKGKKAQGVLADGQWMFEG"
BASE COUNT          306 a          607 c          636 g          348 t
ORIGIN      
        1 gagatgatca gctcgtcggc tccggtccgc tcgatcaggt cgaccagccc ggcctgcacg
       61 gtttcgggac tgccgaatat atggcccttg gccacggaag cgacgactgc ttcctcatcc
      121 ggggtgaaat catgcgccgc cgcctcttcg ggagtgggga accggcctgg ccggccgctc
      181 cggagccggg cgaaagcaag ccgcgaggga ccggccagcc agcgggcttc ctcgtcggtt
      241 tcggcgcaga tcacccccac cccgatcatc accttcggtt cttccagaaa ggcggacggc
      301 tcgaagcggg agcggtagag ctgaaccgcg gcctcggtgt tgccggcgct gaagtgatgg
      361 gcgaaggaat agggcatgcc gagcatcccc gcgacctgag cggtgtagcc gcttgaacca
      421 agcagccaga tctccggcat ggctccttct ccgggaaccg cagtgatttg ctggtaggga
      481 tggccggagg gccattcacc ggtgaagaaa ccgagcagtt caccgagttg ccggggcaga
      541 tcctcgtcgg agagcgggtc gagtccgcgg cgcagggcgg cggccgtaac ctggtcggtg
      601 cccggcgccc ggccgatccc gaggtcgatc cggcccggat tcagcgactc gagggtgccg
      661 aactgctcgg ccacgaccag cggctggtgg ttcggcagca tcaccccgcc cgaaccgacc
      721 cggatcgttt cgctgttggc tgccaggtgg gcgatgatca ccgccggagc ggaactggcg
      781 atcccggtca tgttgtgatg ctcggcaacc cagtggcggt ggtatccgag ccgctccgcc
      841 gcccggacca gttcggtcga gttggcgagg gcggttaccg ggctggcccc ctcgaccacc
      901 ggcgccaggt ccagcaccga gagtttcgga tttccgcgcg gcgcggacgg ttcgctcatc
      961 cctcgaacat ccattgtccg tcggccagga ccccctgtgc cttcttgccc ttgggcggat
     1021 agtcgatctc gaggttttct tccggcgagg gatcggcccg ccagctccac cagctgatcc
     1081 ccttcagcca gtccgagccg gaaagggcct tgaaggtggc ctcgtaggca agtgcctgaa
     1141 tgttctggtc ggccggctgg tcggtcacct cgtaaggctt gacggtggcg cccacctggc
     1201 tcgggtagcc gacctcggta agcaggatcg gccgctccca ctgcgcggac ttcgcctgga
     1261 gctggctggc gatcccctgc caggcggtca ccagttcctc gagagagggc gccttgctga
     1321 tgtcccgggt caacgggtag taggcgtcga ccccgatcgc atcgaggtcg tcccagaatt
     1381 gaacctgatc gacttcgtcc cagttggcgg cgtaggtgat cgggcccgtg aagcggtcac
     1441 ggaccgtctg gatcaccgcc cgccagcgat cggtctccga ggacatcgtc ttgagctcgg
     1501 tcccggccac gaagagatcg accgaatggg aggtcgaaaa cgacgcgaaa tggtcgatga
     1561 aggtgccgta ggaacggaac caggcttcgc ggtcggcggg ctggatgtca ccgcggtagg
     1621 aatcatcgat cacatcgaca tgcggcttca tgatgacctg catcccggcc tgcctgatcc
     1681 agctgatcac cttcgcgagg ctctcgtcgg atggggactt ttcctcgtcc ggctcgatcg
     1741 cgttggcgct cgaactcttc atgtaccagg tcggcaccag ggttatcgcg gttccgccca
     1801 ggtcagcgag ggtctttatc gcttttcgga ccgacggctt cgagtagccg tcgctggtgt
     1861 aggcggtgag attcacgccc agcagcggtc cttcgat
//
LOCUS       DAOQKN010000305         1892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_51139_length_1892_cov_5.672292, whole genome shotgun sequence.
ACCESSION   DAOQKN010000305 DAOQKN010000000
VERSION     DAOQKN010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51139_length_1892_cov_5.672292"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="PLJ64_12760"
     CDS             <1..449
                     /locus_tag="PLJ64_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNC94401.1"
                     /translation="VTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDD
                     TVGRVKAYESIVKGENIPEPSIPESFKVLLKEMQALSLDANVVSEEGVGDLAEEEDDL
                     LQAAQDLGMDLTEVSSGNGAADESLTEEDGDATTDDSGDTEEAGAN"
     gene            458..>1892
                     /locus_tag="PLJ64_12765"
     CDS             458..>1892
                     /locus_tag="PLJ64_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNC94402.1"
                     /translation="MIDINNFDAIEIGLASSKKVRSWSWGEVLKPETINYRTLKPEKD
                     GLFCERIFGPTKDWECYCGKYKRVRYKGIVCERCGVEVTRSKVRRERMAHIDLAAPVS
                     HIWFFKGVPSRIGYLIDMAPKELEKVLYFAASMITWVDEEARAKDMGTLEKEVDSVIA
                     EYESERQSATQELDEALKRRTKYLEDGTQTKFDDDDHLWADSLGLTAAQLRKLSDEDR
                     AKQIKELTKNFEAEINDTEAYIEEAIERLQEVWKIFQTMKPKDVINDETVFRELKDRF
                     GSPFGWGEYFRGGMGAESVRDLLEQIDLEATCEELEDQINTAKGQKQARAVKRLKVAS
                     AFLKSENKPEWMILDCVPVIPPELRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLL
                     DLGAPEIIVNNEKRMLQESVDALFDNGRRGRPVTGPGNRALKSLSDMLKGKQGRFRQN
                     LLGKRVDYSGRSVIVAGPSLKLHQCGLP"
BASE COUNT          396 a          598 c          609 g          289 t
ORIGIN      
        1 tcgtcaccca gcagccgctg ggcggcaagg cgcagttcgg cggccagcgt ttcggcgaga
       61 tggaagtctg ggccctcgag gcctacggcg ccgcctacac gctgcaggag atgctcaccg
      121 tcaagtccga cgacacggtc ggccgcgtca aggcctacga gtcgatcgtc aagggcgaga
      181 acatccccga gccgtcgatc cccgagtcgt tcaaggtcct gctcaaggag atgcaggcgc
      241 tctcgctgga cgccaacgtg gtgtccgagg aaggcgtcgg cgacctggcc gaggaagagg
      301 acgacctgct gcaggccgcc caggatctcg gcatggacct gaccgaagtg agttccggaa
      361 acggtgccgc agatgaatcc ctcactgaag aggacggtga cgccaccaca gatgactcag
      421 gcgacaccga ggaagcaggt gctaactgat gagtaacgtg attgacatca acaacttcga
      481 cgcgatcgag atcggtctcg cctcgtcgaa gaaggtccgg tcctggagct ggggcgaagt
      541 cctcaagccg gagaccatca actaccggac cctgaagccg gaaaaggacg gcctcttctg
      601 cgagcgcatc ttcggtccga ccaaggactg ggagtgctac tgcggcaagt acaagcgggt
      661 ccgctacaag gggatcgtct gcgagcgctg cggcgtcgag gtgactcggt ccaaggttcg
      721 ccgtgagcgc atggctcaca tcgacctggc cgctcccgtt tcgcacatct ggttcttcaa
      781 gggtgtgccc tcgcggatcg gctacctgat cgacatggcg ccgaaggaac tggagaaggt
      841 cctctacttc gccgcctcga tgatcacctg ggtcgacgaa gaggcccgtg ccaaggacat
      901 gggcacgctg gagaaggaag tcgactcggt catcgccgag tacgagtccg agcgccagtc
      961 cgccacccag gaactcgatg aggcgctgaa gcgccgcacc aagtacctcg aggacggcac
     1021 ccagaccaag ttcgacgacg acgatcacct ctgggccgat tcgctcggcc tgaccgccgc
     1081 ccagctgcgc aagctgagcg acgaagatcg cgccaagcag atcaaggaac tcaccaagaa
     1141 tttcgaggcc gagatcaacg acaccgaggc gtacatcgaa gaggcgatcg agcggctgca
     1201 ggaagtctgg aagatcttcc agaccatgaa gccgaaggac gtgatcaacg acgagaccgt
     1261 cttccgtgag ctcaaggacc gcttcggcag cccgttcggc tggggcgagt acttccgcgg
     1321 cggcatgggc gccgagtcgg tgcgcgacct gctcgagcag atcgatctcg aagcgacctg
     1381 cgaggaactc gaggaccaga tcaacacggc caagggccag aagcaggccc gcgcggtcaa
     1441 gcgtctgaag gtcgcctcgg ccttcctcaa gtccgagaac aagcccgagt ggatgatcct
     1501 cgactgcgtc ccggtgatcc cgccggagct caggccgatg gtccagctcg acggtggacg
     1561 cttcgcgacc tctgacctga acgacctcta ccgccgggtg atcaaccgga acaaccgcct
     1621 caagcggctt ctggacctcg gtgcgccgga gatcatcgtc aacaacgaga agcgcatgct
     1681 gcaggagtcg gttgacgccc tgttcgacaa cggccgccgc ggccgtccgg tcaccggacc
     1741 gggcaaccgg gcgctgaagt cgctctcgga catgctcaag ggcaagcagg gccggttccg
     1801 ccagaacctg ctcggcaagc gcgtcgacta ctcgggtcgt tcggtcatcg tggccggccc
     1861 gtcgctgaag ctgcaccagt gcggtctgcc ga
//
LOCUS       DAOQKN010000306         1851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_53295_length_1851_cov_3.630290, whole genome shotgun sequence.
ACCESSION   DAOQKN010000306 DAOQKN010000000
VERSION     DAOQKN010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53295_length_1851_cov_3.630290"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..485)
                     /locus_tag="PLJ64_12770"
     CDS             complement(54..485)
                     /locus_tag="PLJ64_12770"
                     /inference="COORDINATES: protein motif:HMM:NF022030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94403.1"
                     /translation="MGEIVIGAMASMLICLFLAPRFIDFLRGREFGQHIREEGPAGHQ
                     TKAGTPTMGGLIIFTAIIVPFLVLSERDAASMTVFGVALGCAALGFADDYIKIIKRRS
                     LGLPGRYKLLGQVVLALILWWVARHEVGLEPVIHFRIGDLT"
     gene            complement(478..>1851)
                     /gene="murF"
                     /locus_tag="PLJ64_12775"
     CDS             complement(478..>1851)
                     /gene="murF"
                     /locus_tag="PLJ64_12775"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNC94404.1"
                     /translation="VIARGSGGVPERAEIDSRKVEGGELFFGLTGGQSDGGEHAANAL
                     ESGAWGVVVSHERAAELVAGGPEPGNSGAPGAFVFASPDPLLSLQMLARAWRRELDAT
                     VIGITGSVGKTSVKDITRALLPGEVHASAENFNTEIGLPLTILEAPAGTETLVLEMAM
                     RGEGQIAELAVIAEPDVGVITNVGPVHVELLGSIEAVAAAKAELIVNMRPEGVMIVPF
                     EAGYLDPHLENVPGLIRFGGEGDVRVIELSVENGATKARIETLQGSQAFEFPFAEPHN
                     LTNSLAAIGAGVAAGFTPAELAARADGIAFSRFRNEHIDLGDRGLIINDCYNANPVSM
                     RAALEYLAQIDRPRKIAVLGLMAELGPDEVRFHEEIGEIARGLEVDVISVGDLARAYG
                     ADLKAGDPEEAARLVDERLGPDLAVLVKGSRAAGLERVTELVTATAKGTNAETAAASS
                     ESEAAGG"
BASE COUNT          341 a          611 c          582 g          317 t
ORIGIN      
        1 gccccggcca gaaccaggaa gacgaggatg aagtaggcga ccgggccgat gttctaggtc
       61 aagtcgccga tccggaagtg gatcaccggt tcgaggccga cctcgtggcg ggccacccac
      121 cagaggatca gggcgagaac gacctggcca agcagtttgt agcggccggg caggcccagc
      181 gaacggcgtt tgatgatctt gatgtagtca tcggcaaagc caagggcggc gcaaccgagg
      241 gcaacgccga agacagtcat cgaagccgcg tcccgctcgg aaagaacgag gaagggcacg
      301 atgatcgcgg tgaagatgat cagtccgccc atggtcgggg tgcccgcctt ggtctggtga
      361 ccggccgggc cttcctcgcg gatgtgctga ccgaattcgc gtccgcgcag gaaatcaatg
      421 aagcgtggcg cgaggaagag acagatcagc atcgaggcca tcgccccgat cacgatttca
      481 cccaccggcg gcctcgcttt ccgatgaagc tgcggccgtt tcggcattcg tgcctttggc
      541 cgtggcggtg accagctcgg tcacccgctc gagtccggcc gcccgggaac ctttgacaag
      601 tactgccagg tcgggaccaa gccgctcgtc gaccagccgg gcggcctctt cgggatcacc
      661 tgctttcagg tccgcgccgt aggctctggc gagatctccg acgctgatca catcgacttc
      721 aagcccgcgg gcaatttccc cgatctcctc gtgaaagcga acctcgtcgg ggccgagttc
      781 agccatcaga ccgagcaccg cgatcttgcg cggccggtcg atctgggcca gatactcgag
      841 ggcggcacgc atcgacaccg gatttgcgtt gtaacagtcg ttgatgatga gaccccggtc
      901 accgaggtcg atgtgttcgt tgcggaagcg tgaaaaggca atgccatccg cccgggctgc
      961 cagttccgcc ggggtgaatc cggccgccac accggcaccg attgcggcca gggaattggt
     1021 caggttatgc ggttcggcga agggaaattc gaaggcctgc gaaccctgaa gggtctcgat
     1081 ccgggccttg gtcgccccgt tttcaaccga gagttcgatc acccgcacat caccctcccc
     1141 gccgaagcga atcaggcccg gcacgttttc gaggtgcggg tcgaggtaac cggcctcgaa
     1201 gggaacgatc atcactcctt cgggtctcat gttgacaatc agctctgcct tggccgcggc
     1261 caccgcctcg atcgaaccga gcagttcgac gtgaacaggc cccacattgg tgatcactcc
     1321 cacatccggt tcggcgatca ccgccagttc ggcaatctgt ccctcgcccc gcatcgccat
     1381 ttccaggacc agggtctcgg ttccggccgg cgcttcgaga atggtcaagg gcaggccgat
     1441 ctccgtattg aagttctcgg ccgaggcgtg gacctcaccg ggcagcaacg cccgggtgat
     1501 gtccttgacc gaggtcttgc ctaccgatcc agtgattccg atgaccgtcg catcgagttc
     1561 cctgcgccag gcgcgggcca gcatctgaag tgaaagcagg gggtcggggc tggcgaaaac
     1621 aaatgcgccg ggcgcaccgg agttgcctgg ctccggcccg ccggcgacca gctctgccgc
     1681 ccgttcgtgg cttacgacga ccccccaggc cccggactca agcgcgttcg ccgcgtgctc
     1741 gccgccgtcg ctctgacctc cggtcaggcc gaagaagagc tcgcctcctt caaccttgcg
     1801 agaatcgatc tcggcccgct ccggcacccc gcccgacccg cgggcaatca c
//
LOCUS       DAOQKN010000307         1845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_53630_length_1845_cov_3.850838, whole genome shotgun sequence.
ACCESSION   DAOQKN010000307 DAOQKN010000000
VERSION     DAOQKN010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53630_length_1845_cov_3.850838"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="PLJ64_12780"
     CDS             <1..830
                     /locus_tag="PLJ64_12780"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNC94405.1"
                     /translation="ELNITDPDTAVSDLVLTTKSYGDLIESSTYIEQIQSRNESIVTR
                     VRSLRDQQEAIVVRLKKAKDRIEAARDSIAAEKQNLTTARQAVQDQQAKLAAARADRQ
                     AQVDSIDTKVDHLEDIEADLQQKIAEQIAAASGVSVLPAGPMTSPSAAGLIWPVSGTV
                     TSGFGGRSSPGGIGSTYHEGLDIAVPEGTPIRAAASGTVIMASYNGGYGNYTCIDHGS
                     GLSTCYGHQSSFAVSAGQSVSQGDVIGYSGNTGSSTGPHLHFEVRINGVAQDPLGYL"
     gene            complement(1197..>1845)
                     /locus_tag="PLJ64_12785"
     CDS             complement(1197..>1845)
                     /locus_tag="PLJ64_12785"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNC94406.1"
                     /translation="VNDCVEAHGMRPAAYLDSLGMLTPTTTLAHGVWLDREEIELIAD
                     RGATVTSNPVANMKLAVGGVFPYPEAARAGVPVALGTDGAGSNNSLDLLADLKVFALA
                     QRHAAADAKAIPKDEAWGVATGQRSALIGGLGRPALPASDPLTVGAPADFIVLDPATP
                     ELALGNLASSLVYSATGSAVKDTVVDGKVLMRDRQVDGHEEILTKSAERARRLGL"
BASE COUNT          313 a          621 c          624 g          287 t
ORIGIN      
        1 tcgaactgaa tatcacggat ccggatacgg ccgtcagcga cctggttctg accaccaaga
       61 gctacggcga cctaatcgaa agctcgacct acatcgagca gatccagagt cgcaacgagt
      121 cgatcgtcac ccgggtccgg agcctccgtg accagcagga agcgatcgtc gtccgtctga
      181 agaaggcgaa ggacaggatc gaggcggccc gcgactcgat cgccgctgag aagcagaacc
      241 tcaccacggc ccgtcaggcg gtccaggacc agcaggccaa gctggccgcg gcccgggccg
      301 accgccaggc ccaggtcgac agcattgaca ccaaggtcga tcacctcgaa gacatcgagg
      361 ccgacctcca gcagaagatc gccgaacaga tcgctgccgc cagcggagtg agcgtgctgc
      421 ccgccggccc gatgacttca ccctccgccg ccggcctgat ctggccggtc agcggcaccg
      481 tcacttccgg tttcggcggc cgcagctcac cgggcggcat cggctcgacc taccacgagg
      541 gacttgacat cgccgtgccc gaaggcactc cgattcgggc ggccgccagc ggcacggtga
      601 tcatggcctc ctacaacggc ggctacggca actacacctg catcgaccac ggctcggggc
      661 tctccacctg ttacggccac cagtccagct tcgccgtctc ggccggccag tcggtcagcc
      721 agggcgacgt aatcggctac tccggcaaca ccggctcatc aaccggcccc cacctccatt
      781 tcgaggtccg catcaacggc gtagcccagg atcctctcgg ctacctgtag ggctcgggca
      841 aagtccgcag gaaccaccag ccgcccgctg gacttcgtcg gacgctcgac gcctcgggaa
      901 cgtacgaaaa gtacgccccc ttcaccgcct tcgcttgcct ccttgccagc gaacggctgg
      961 aggcccctgc gaactacccg ctggaggttg gtggggaagg tgccgcaggt ttgcaaccgc
     1021 agaggtcgca aacctgcgtc accgtcctcg atttcgggtt ggtgtagccg tggggtcggg
     1081 tggccctccc aaagtgcctt cgcgatttct gctccgatca tcgagcaggc acgcgttcac
     1141 gcgacggtag gggcatcttt gggagggcca cccggcccca cggcgtaaac cgtcctctag
     1201 aggccgaggc ggcgggcgcg ttcggcggat ttggtgagga tttcctcgtg cccgtctacc
     1261 tggcggtctc gcatgaggac tttgccgtcg acaacggtgt cctttacggc cgagccagtg
     1321 gccgaataga caaggctcga ggcgaggttg ccgagggcga gttccggggt ggccggatcg
     1381 aggacgatga agtcggcggg ggcgccgacc gtcagcgggt cggaagccgg gagggcgggt
     1441 cggccgaggc cgccgatcag ggcggagcgc tggccggtgg cgacccccca ggcttcgtcc
     1501 ttggggatcg ccttggcgtc ggccgccgcg tggcgctggg cgagggcgaa gaccttgagg
     1561 tcggccagca ggtcgaggga gttgttcgag ccggcgccgt cggtcccgag tgcgaccggg
     1621 acgccggccc gggccgcctc ggggtagggg aaaactccac ccacggccag cttcatgttc
     1681 gcgaccgggt tgctggtgac cgtggcgccg cggtcggcga tcaattcgat ctcttcgcgg
     1741 tcaagccaga ccccgtgggc gagagtggtc gtcggggtca gcatgccgag gctgtccaga
     1801 taggcggcgg gccgcatgcc gtgggcctcg acgcagtcgt tcacc
//
LOCUS       DAOQKN010000308         1844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_53691_length_1844_cov_3.217999, whole genome shotgun sequence.
ACCESSION   DAOQKN010000308 DAOQKN010000000
VERSION     DAOQKN010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53691_length_1844_cov_3.217999"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="PLJ64_12790"
     CDS             complement(<1..237)
                     /locus_tag="PLJ64_12790"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNC94407.1"
                     /translation="MSKGHEAFKGQLPAYLLGALDPGERADLEHHLDGCAECKAELTW
                     LEPATATLAAGVEQFEPSSDLKARVMAAVDADLAE"
     gene            complement(234..824)
                     /locus_tag="PLJ64_12795"
     CDS             complement(234..824)
                     /locus_tag="PLJ64_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNC94408.1"
                     /translation="MSNKVPAQPGDPDENLLVRVGGRDKQAFELLYDRFERRAFSLAY
                     RIVGERSLAEDIVQEAFLSIWRNAARYDPDRGSAGAWALGIVRNRAIDVLRSRSSRTP
                     DLDHDDDSTLENRASPLKTEEEAMQREQGREIRSRISELPENQSKVIGLAFYGGFTQT
                     EISDMLELPLGTVKGRMRLGMEKLRVELSSYEGERA"
     gene            complement(862..1530)
                     /locus_tag="PLJ64_12800"
     CDS             complement(862..1530)
                     /locus_tag="PLJ64_12800"
                     /inference="COORDINATES: protein motif:HMM:NF025049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNC94409.1"
                     /translation="MNELKTEIPAELPADTEMVHPELAAIEVDGSERSDVLMKGALAA
                     GAIFGLGAISPFVRNAMAANGNKGDIEILNYALTLEYLESAFYAEALKKAGLSGDLKQ
                     LVTTISTDEDAHVKAISQTIKDLGGKPVKAPKVEFPYSDTAGFTALAETIENVGVSAY
                     NGAAPMISSKDVLEAAGSIVQVEARHAAAIALQNGNEPAPDAFDKPMTQAQVLKAVQP
                     FLVS"
     gene            complement(1558..>1844)
                     /locus_tag="PLJ64_12805"
     CDS             complement(1558..>1844)
                     /locus_tag="PLJ64_12805"
                     /inference="COORDINATES: protein motif:HMM:NF025049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNC94410.1"
                     /translation="APKPKTEFPLNNAQQVLRLASTVENLGAAAYLGQAARIQSPDVL
                     AAALSIHSVEGRHAAALNTLLGKKPVPDGPFAQPAPAGMVLNAVQPFIVG"
BASE COUNT          298 a          602 c          556 g          388 t
ORIGIN      
        1 ctctgccagg tcggcgtcga ccgcggccat cacccgcgcc ttcaggtcgg aggacggctc
       61 gaactgctcg accccggctg cgagcgtggc agtggctggt tcgagccagg tcagctccgc
      121 cttgcattcg gcgcatccat cgaggtggtg ctcgaggtcg gcccgttcac ccggatcgag
      181 cgcacccagc aagtaagccg gcaactgtcc cttgaaagct tcgtgtccct tgctcatgcc
      241 ctctctcctt cgtatgagct caattcgacc cggagttttt ccatcccgag tctcatccgt
      301 cccttgaccg tgccgagcgg taattcgagc atgtcggata tctcggtctg ggtgaaccct
      361 ccataaaagg cgagtccgat cacctttgac tgattttccg gcagctccga aatccgcgac
      421 ctgatctccc ggccctgttc cctctgcatc gcttcttcct ccgtcttcag cggcgacgcc
      481 cggttttcca gcgtggagtc gtcatcgtga tcgagatccg gggtccggct cgatcggctc
      541 cgcagcacgt cgatcgcgcg gttgcgcacg atcccgaggg cccacgcccc ggcgcttccc
      601 cgatcagggt cataccgggc ggcgtttctc cagatcgaaa gaaaagcctc ctggacgatg
      661 tcctcggcca gtgaccgttc gccgacgatc cggtaggcaa gtgaaaacgc acgtcgttcg
      721 aaccggtcgt ataagagttc gaatgcctgc ttgtcgcggc cgccgacccg gacgagaagg
      781 ttctcgtccg ggtcgcccgg ctgcgcggga accttgttac tcatttccat gtcaggttcc
      841 cgcattccct acccgggaat ctcagctgac caggaagggc tgaaccgctt tcagcacctg
      901 ggcctgggtc atcggtttgt cgaaggcatc cggcgccggc tcattgccgt tctgcagggc
      961 gattgccgct gcgtgcctcg cctcgacctg gacgatcgag cccgccgcct ccagcacgtc
     1021 cttcgaactg atcatcggag cggcaccgtt ataggcgctc accccgacgt tctcgatcgt
     1081 ctcggcaagg gctgtgaagc cggcagtgtc cgaatacggg aactcgacct tgggcgcctt
     1141 gaccggcttg ccacccaggt ccttgatcgt ctgcgagatc gccttcacgt gggcatcctc
     1201 atcggtcgag atggtagtca ccagttgctt gaggtcaccc gaaaggccgg ccttcttcag
     1261 ggcctccgcg taaaacgcgg actcgaggta ttcgagggta agcgcgtagt tgaggatctc
     1321 gatgtccccc ttgttgccgt tcgcagccat cgcgtttcgg acgaaggggc tgatcgcccc
     1381 gaggccgaaa atcgcaccgg cagccagggc gcccttcatc agaacgtcac tgcgttcgct
     1441 gccatcgact tcgatcgcgg ccagctcggg gtggaccatt tcagtgtcgg cgggaagttc
     1501 cgccgggatt tctgtcttca gttcattcat ctgaatcttc ctttccggtc agccggttca
     1561 gccgacgatg aagggctgga cggcgttcag aaccattccc gccggggcag gctgggcgaa
     1621 gggcccatcc ggaacaggtt tcttgccgag cagcgtgttg agcgccgctg cgtgacggcc
     1681 ttcgacactg tggatcgaga gcgccgccgc gagaacgtcc ggactctgga tccgggcggc
     1741 ctgaccgagg taggccgcag caccgagatt ctcgaccgtt gaagcgagcc gcagaacctg
     1801 ctgtgcgttg ttcagcggga actcggtctt cggcttcggt gccg
//
LOCUS       DAOQKN010000309         1807 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_55689_length_1807_cov_3.686644, whole genome shotgun sequence.
ACCESSION   DAOQKN010000309 DAOQKN010000000
VERSION     DAOQKN010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55689_length_1807_cov_3.686644"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /gene="mraY"
                     /locus_tag="PLJ64_12810"
     CDS             <1..637
                     /gene="mraY"
                     /locus_tag="PLJ64_12810"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNC94411.1"
                     /translation="QVVLALILWWVARHEVGLEPVIHFRIGDLTWNIGPVGYFILIFL
                     VLAGATNGVNLTDGLDGLAAGSSAIVLLTYTAISYVTVGQHDLALFAVCLVGGCIGFL
                     WFNAFPATIFMGDTGSLGLGGAIGALAVMTQTEVLLIVIGGIFVIEALSVLIQVFSFK
                     TFGRRVLMMAPLHHHFEMMAWSETKIMLRFWIIAAVCSGIGFAIYQHSIGR"
     gene            1190..>1807
                     /locus_tag="PLJ64_12815"
     CDS             1190..>1807
                     /locus_tag="PLJ64_12815"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNC94412.1"
                     /translation="MEYVRSSKARPPLPQGPFLVVGLARSGVAAARALKAHGEAVFGV
                     DSGEPEGLELLRESGIDFETSVPGTDRLDGVATVIKSPGVPESAEVIDEASRRGLPVI
                     GELELGWRMFEAPVVAITGTNGKTTTTELTAHLFRSAGRPVSAAGNVGHPVCEVAMEA
                     AAGREVGTVVCECSSFQLEDSVEFSPEVAVFLNLGPDHLDRHGDMG"
BASE COUNT          286 a          585 c          569 g          367 t
ORIGIN      
        1 ccaggtcgtt ctcgccctga tcctctggtg ggtcgcccgt cacgaagtcg gccttgaacc
       61 ggtgatccat ttccggatcg gtgacctgac ctggaacatc ggtccggtcg ggtatttcat
      121 cctgatcttc ctggttcttg ccggcgccac gaacggagtg aacctgaccg atgggctaga
      181 cggcctggcg gccggttcca gtgcgatcgt cttgctcacc tacacggcga tctcctatgt
      241 gacggtcggt cagcatgacc tcgcgctctt cgccgtctgc ctggtcggcg gctgcatcgg
      301 tttcctttgg ttcaacgctt tcccggccac cattttcatg ggggataccg gctcgttagg
      361 tctcggcgga gccattggtg cgctcgccgt gatgactcag accgaggtgc tgctgatcgt
      421 gatcggcggg atcttcgtga tcgaagccct ctcggtcctg atccaggtct tctccttcaa
      481 gaccttcggt cgccgggtcc tgatgatggc cccgctccac catcacttcg agatgatggc
      541 ctggtcggaa accaagatca tgctccgctt ctggatcatc gccgccgtct gctccggaat
      601 cgggttcgcg atctaccaac attcaatagg ccggtagccc gggcggcgcc gggagccggg
      661 cggagccagg aaccaccagc tgtccgctgg ccttcgtcaa cggccggaaa cgacagctca
      721 cgtacccgcg tactatcgcg gtcatttccg gccttgttcc tcggcagcga acacctggag
      781 gcccctggca tccacccgct gaggggttga gggtgttggc attcttcgag aacccgcttt
      841 tcggcgtata gcgcacccaa ctccgatctc gacggtatcc cgaccctgat ccggggcagg
      901 tgcctatcgg agttgtctat atagacaact cacatacccg cctcccgaga acgagggttc
      961 aaacccggcc ctggaggtgt cgcgggactc ggggccacct tcaaatcccc cgtcacctgc
     1021 ccggttcaac ccaagtgggt cggattctcg gcagtcgggc cggaaatccg acccacctga
     1081 actcatggtg taaccgggcc tgtcccgggc cgatttcgtg gccgatgaac cgggcgaaag
     1141 aattcggctt tggggcaggt gatcggcttc ccgtggggaa gactcctgag tggagtatgt
     1201 ccgctcatcg aaggcccgcc cgccgcttcc ccaagggcct ttcctcgtag tcgggctggc
     1261 acgctccggg gttgccgccg ccagggcgct gaaagcccac ggtgaagccg ttttcggggt
     1321 tgactccggc gagcccgaag gccttgagct gctgcgtgag tcaggcatcg actttgagac
     1381 ctccgtcccg gggaccgatc ggctcgacgg ggttgccaca gtgatcaaga gcccgggcgt
     1441 gcccgagtcg gccgaagtga tcgacgaagc gtcccggcgc gggctgccgg tgatcggtga
     1501 actcgagctc ggctggcgca tgttcgaagc accggtcgtc gcaatcaccg ggaccaacgg
     1561 caagacgacc acgaccgagt tgaccgccca ccttttccgc tcggccggcc ggccggtgtc
     1621 tgccgccggc aacgtcgggc atccggtctg cgaggtagcg atggaagcgg cggctggacg
     1681 ggaggtcggc accgtggtct gcgaatgctc cagcttccag ctcgaggatt cggtcgagtt
     1741 ctcgcccgag gtcgcggtgt tcctcaatct gggtccggat catctcgacc gccacggcga
     1801 catgggc
//
LOCUS       DAOQKN010000310         1796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_56299_length_1796_cov_5.036760, whole genome shotgun sequence.
ACCESSION   DAOQKN010000310 DAOQKN010000000
VERSION     DAOQKN010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56299_length_1796_cov_5.036760"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="PLJ64_12820"
     CDS             complement(<1..357)
                     /locus_tag="PLJ64_12820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94413.1"
                     /translation="MAAFTLTGSAFAVSGCGGSGEEPKQWPNDAGYPFDEVKLAADGV
                     PRTAKLTADQKAEILRIARNDPLVKELLEGHEVEWRKPYPWSRPNMELLGGGVDVHFA
                     EPVDLSGATIPAIENIE"
     gene            521..1732
                     /locus_tag="PLJ64_12825"
     CDS             521..1732
                     /locus_tag="PLJ64_12825"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC94414.1"
                     /translation="MSDAEKIVIVGASLTGARAAEGAREAGFEGEIVLIGDEPERPYE
                     RPPLSKDYLRGEKDDLAWVHEESFYAEHDIDLRTSTFVSGIDPSAGTVLVEGGDPEPY
                     DRLLLATGCEPRRIDIPGAELDGIHYLRSAGDSAELGERFREGTRLVVVGAGWIGSEV
                     AASARQKGCEVTLVEPQEVPLQSVLGTEVGGVYAEIHLEQGVDFKSGTGVEAFLGEGS
                     VSKVRTTAGEIEADLVVVGVGAVPRTGLAEAAGLELNNGVPVDAGLRTEAEGIYAAGD
                     IANQFHPFFNDRIRVEHWANARRQGFAAGRSMAGAEVIYDDLPYFFSDQYDVGMEYVG
                     FASDWDEVVIRGSKENREFIAFWLKDGRIQAGMNVNIWDVSETIGELIKSKRQIDLVA
                     LADPDTDLATI"
BASE COUNT          315 a          551 c          621 g          309 t
ORIGIN      
        1 ctcgatgttc tcgatggcgg gaatcgtggc gccagacagg tcgaccggct cggcaaaatg
       61 gacatcgact cctcctccca ggagttccat gttcggacgg gaccaggggt agggcttgcg
      121 ccactccacc tcatggcctt ccagtagttc cttcacgagc ggatcgttcc tggcgattct
      181 caggatctcg gctttctggt ccgcagtgag tttcgcggtt cggggtacac cgtctgcagc
      241 cagcttcact tcgtcaaacg gatagcccgc gtcgttcggc cactgtttcg gttcttctcc
      301 gctgccgccg caacccgaaa ccgcgaatgc cgatcccgtc agggtgaagg cggccacgat
      361 cagggtgacg ctccggcgga tggggaacct ctcttctgat cgcaatggca tttcctctgg
      421 gtcctggatc cggtctctgc tgccggtggc agtgattcag gttacgggcc ggagagatcg
      481 ggggagtagc tttgaagaac agacctgaac ggaggcggag atgagtgatg ccgagaagat
      541 cgtgatcgtc ggggcgagcc tgaccggggc gcgggcggcc gagggtgcgc gggaggccgg
      601 attcgagggc gagatcgtgc tgatcgggga cgagcccgag cggccctacg aacggccgcc
      661 gctttcgaag gactatctgc gaggcgagaa ggacgacctc gcctgggtcc atgaggagtc
      721 gttctatgcc gaacacgaca tcgacctgcg gacctcgacc ttcgtctccg ggatcgatcc
      781 ctcggcggga acagttctgg tcgagggggg cgacccggag ccgtatgacc ggctgctgct
      841 ggcaacgggc tgcgagcccc gccggattga catccccgga gccgaactcg acggaatcca
      901 ctacctgcgc agtgcaggcg actcggccga actcggcgag cggttccggg agggaaccag
      961 gctggtcgtg gtcggcgccg gctggatcgg ctccgaagtc gccgcctcgg cccggcagaa
     1021 gggctgcgaa gtcaccctgg tcgaaccaca ggaggttccg ctccagtcag tgctggggac
     1081 cgaggtcggc ggggtgtacg cggagatcca tctcgagcag ggtgtcgact tcaagtccgg
     1141 caccggggtc gaggccttcc tcggcgaagg ctcggtctcg aaagtccgga ccacggccgg
     1201 cgagatcgaa gccgacctcg tcgtggtcgg cgtcggtgcg gtgccgcgga ccggcctcgc
     1261 cgaggcggcc gggctcgagc tgaacaacgg ggttccggtc gatgccggcc tgcggaccga
     1321 ggcggagggg atctacgcgg ccggtgacat cgccaaccag ttccatccct tcttcaacga
     1381 ccggatccgg gtcgagcact gggccaacgc ccggcgccag ggcttcgccg cgggtcggtc
     1441 aatggccggg gccgaagtca tctacgacga cctgccctac ttcttctccg atcagtacga
     1501 cgtcggcatg gagtacgtcg gtttcgccag cgactgggac gaggtggtga ttcgcggcag
     1561 caaggaaaac cgcgagttca tcgccttctg gctgaaggac ggccggatcc aggccgggat
     1621 gaacgtgaat atctgggacg tcagcgagac gatcggcgag ctgatcaagt caaagcggca
     1681 gatcgacctc gtcgccctcg ccgaccccga caccgacctc gccacgatct agaggaccgc
     1741 aaacccggag agccggaagg ccgacgcctt cttccagtgg attaattggg tgcaag
//
LOCUS       DAOQKN010000311         1793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_56486_length_1793_cov_3.459148, whole genome shotgun sequence.
ACCESSION   DAOQKN010000311 DAOQKN010000000
VERSION     DAOQKN010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56486_length_1793_cov_3.459148"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="PLJ64_12830"
     CDS             complement(<1..488)
                     /locus_tag="PLJ64_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94415.1"
                     /translation="MILDGARAAFAADPSRTAVADGSGSWSWREFLEDVERTAADLGE
                     SAGEPVEIRLPSSKRFLARFFAAAELRRPACTLHTDWAGPELEAAITAARSFRPEIPP
                     PEDEDPVFYIGFTSGTSGTPKPFARRSKSWISSFEPAGELFSIEPGDVVYLPGSLQHS
                     HFL"
     gene            complement(485..880)
                     /locus_tag="PLJ64_12835"
     CDS             complement(485..880)
                     /locus_tag="PLJ64_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HNC94416.1"
                     /translation="MTESRSESRPFSYAVLRVVPCLERGERLNVGLALFCRQHDFLEL
                     ETSLDRKRLAAIAPDLDPEPVESRLAVIRRVIEGDEAAGALAELEPSERFGWLTAPSS
                     TIIQSSEVHTGLTADPAAELKRLFESLVL"
     gene            complement(873..1661)
                     /locus_tag="PLJ64_12840"
     CDS             complement(873..1661)
                     /locus_tag="PLJ64_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I and II"
                     /protein_id="HNC94417.1"
                     /translation="MPGTVTENAVSELRRVDAIRYVTALREGGSLPGLVEGDDDGTWV
                     VKFHGAGQGPGALVSEVVAGEIARRIGLPVPELVLVEVAPELGYAEPDPEIQELIEAS
                     PGLNLGMDFLPGSLPFALSPDDPFDPELAADIVFFDALVTNIDRTPRNPNLLTWHGQT
                     WLIDHGAAFYRQHGAAPLAETATSPTPVLRDHVLLPVAGPLAGAGERLTGRARDAIRP
                     AVELVPDEWLGEDPAARREDFAAFLERRLAGQDHFIAELEGDDD"
BASE COUNT          286 a          591 c          627 g          289 t
ORIGIN      
        1 agaaaatgcg aatgctgaag tgatccgggc aggtagacga catcgccggg ctcgatcgaa
       61 aacagctcgc cggccggttc gaagcttgag atccacgact tcgaccgccg ggcgaacggt
      121 ttcggcgtgc cgctggtgcc ggaggtgaag ccgatgtaga agaccgggtc ctcgtcctct
      181 ggcgggggaa tctccggcct gaagctccgg gcagccgtga ttgcggcctc cagctccggt
      241 ccggcccagt cggtgtgaag ggtgcatgcc gggcgccgga gctcggccgc cgcgaagaac
      301 ctcgccagga accgcttgct ggagggcagg cggatctcga ccggctctcc ggcgctttcg
      361 cccaggtccg cggcggttcg ctcgacatct tcgaggaact cgcgccagga ccaggagccc
      421 gagccgtcgg cgacggcggt tctcgaggga tccgccgcga acgccgcgcg ggcgccatcg
      481 aggatcacaa cacgaggctc tcgaagagcc gcttgagttc cgccgccggg tcggcggtca
      541 ggccggtgtg aacctcggag ctctggatga tggtcgagga gggtgccgta agccagccga
      601 agcgctcgga cggttcgagc tcggccaggg cgccggccgc ctcgtcccct tcgatcaccc
      661 tgcggatcac ggccagccgg ctctcgaccg gctccgggtc gaggtcgggt gcgatcgccg
      721 cgagccgctt gcggtcgagg ctggtttcga gttccaggaa atcgtgctgg cggcagaaga
      781 gggcgagccc gacgttcagc cgttcgcccc gttcgaggca gggaacgacc cgcaacaccg
      841 catagctgaa cggccgggat tcggatcgcg actcagtcat cgtcaccctc gagttcggcg
      901 atgaagtggt cctgccctgc caggcggcgt tcgagaaagg ccgcgaagtc ctcgcgtctt
      961 gcggccggat cttcccccag ccactcatcc ggaaccagct ccacagcagg acggattgcg
     1021 tcccgggccc ggccggtgag ccgctctccg gctccggcga gcggtcccgc tacaggcagc
     1081 aaaacgtggt cgcgaaggac cggggtgggg gaggtcgcgg tttcggccag cggcgcggca
     1141 ccgtgctggc ggtagaaggc cgcgccgtgg tcgatcagcc aggtctgccc gtgccaggtc
     1201 agcaggttcg gattgcgggg ggtgcggtcg atgttggtga ccagggcatc gaagaagacg
     1261 atgtcagcgg ccagttcggg gtcgaacggg tcatcagggg aaagggcgaa gggcagcgac
     1321 ccgggcagga agtccatgcc caggttgagc cccgggctcg cttcgatcag ttcctggatc
     1381 tcggggtccg gctcggcgta gccgagttcc ggggcgacct cgaccagcac cagttcgggc
     1441 accggaaggc cgatccggcg ggcaatctcc ccggccacga cctcggagac cagggcgccc
     1501 gggccctgcc cggcaccgtg gaacttgacc acccaggtgc cgtcgtcatc accctcgacc
     1561 aggcctggca gcgacccgcc ctcgcgcagc gcggttacgt agcggatcgc gtcgacccgc
     1621 ctcagttcac tgaccgcgtt ctcggtgacc gtgccgggca tcgctatagc ttcgcatccg
     1681 ggtaggcttc agccgttctt cacgcctaag aaagggtctc gatgtccacg ctttccaaca
     1741 cggttgactc tccccgtgtt cccgtcctcg ctgatttgtt cgccaatacc tgg
//
LOCUS       DAOQKN010000312         1779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_57358_length_1779_cov_4.060905, whole genome shotgun sequence.
ACCESSION   DAOQKN010000312 DAOQKN010000000
VERSION     DAOQKN010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57358_length_1779_cov_4.060905"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="PLJ64_12845"
     CDS             <1..488
                     /locus_tag="PLJ64_12845"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNC94418.1"
                     /translation="VNNAGIGIGQPIHEIQTKHLDLQVAVNLRALAVATRESVEMLKK
                     AGDEHGKALIVNLASIAGVDSPPWLSIYGATKAAVIGFSKSTQKELMGTGVAVTAICP
                     GFVATDMTEFAQQAVPAEEMMQPEDIAEAVSFLLKVSPACAVPELVMARPVAGPETQG
                     M"
     gene            512..1693
                     /locus_tag="PLJ64_12850"
     CDS             512..1693
                     /locus_tag="PLJ64_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC94419.1"
                     /translation="MRCLVAAFGDPGHVFPAVSLALALKGRGHDVILETWPQWQGVVE
                     GVGLGFVSADQYQVFPPPASGSPGAGQAAVALMRLLEEFRPDVVVNDILTVAPALAAE
                     AHGCAWGTLIPHIYPVQDHGMPLFSVGGLPPRTGIGRGFWSFTERALKFGLERGRDDL
                     NDQRALVGLPPTDRFHGGTSPDLAMVATYPQLEYPRDWPGSVHVTGPMPFETPYEDIA
                     LPPGDEPLILVAPSTSQDPDNRLVRSALAALGDQPVRVVATTNRVVPSRPIEVPANSV
                     LVDWLSYSQLMPESSLVICHGGHGTVARALAAGVPVLTCPAAGDMNETAARVTWAEVG
                     RSIRWSLTGPRTLRWAVAEILNEPLYRARAGQISAWDAEHDGAVRGAELVEALADPGR
                     N"
BASE COUNT          287 a          594 c          588 g          310 t
ORIGIN      
        1 tggtcaataa cgcgggaatc gggatcggcc agccgatcca cgagatccag accaaacacc
       61 tcgatcttca ggtagcggtc aacctgcgcg cactcgctgt cgcgacccgc gagtcggttg
      121 agatgctgaa gaaggcaggc gatgaacacg gcaaggcgct gatcgtcaac ctcgcctcga
      181 tcgccggggt cgactcgccg ccgtggctgt cgatttacgg ggcgaccaag gccgccgtga
      241 tcggcttctc gaagtcgacc cagaaggagc tgatgggcac cggggttgcg gtgaccgcta
      301 tttgtccggg cttcgtcgcc accgacatga ccgagttcgc ccagcaggct gtgccggccg
      361 aggagatgat gcagccggag gacatcgccg aggcggtcag cttcctgctc aaggtttccc
      421 ccgcctgcgc ggtgccggag ctggtaatgg cccgtccggt cgccggcccc gagacccagg
      481 ggatgtagac ctggccgcag gttccggaag tttgcgttgc ctggtcgccg ctttcggcga
      541 tcccggccat gtctttcccg cggtcagcct cgcccttgcg ctcaagggac ggggccatga
      601 tgtgatcctc gagacatggc cccaatggca gggcgtggtc gaaggggtcg gcctgggttt
      661 tgtcagcgcc gatcagtacc aggtgttccc gcctccggct tccggttccc ccggagccgg
      721 ccaggcagcg gtggctctga tgcgactgct tgaggagttc cggcctgatg tggtggtaaa
      781 cgacattctc accgtggctc ccgccctggc cgccgaagct cacggctgcg cctggggaac
      841 cctgatcccg catatctacc cggtccagga tcacgggatg cccctcttct cggtcggtgg
      901 cctgccgcca cgaaccggga tcggccgcgg cttctggagc ttcaccgaac gagccctgaa
      961 gttcgggctg gaacggggac gggatgacct gaacgaccag cgcgcgttgg tcggccttcc
     1021 cccgaccgac cgattccacg gggggaccag tcccgacctg gcaatggtcg ctacctaccc
     1081 gcaactcgag taccctcgcg actggcccgg ttctgtccac gtcaccgggc cgatgccctt
     1141 cgaaaccccg tacgaagaca ttgctttgcc acccggcgat gaaccgctga tcctggtcgc
     1201 tcccagcacc tcgcaggatc ccgacaaccg tctcgtcaga tcagctctcg ctgccctcgg
     1261 cgatcaaccg gttcgggtgg tggcgaccac aaaccgggtc gttccgagcc ggccgatcga
     1321 agtccccgcg aactcggtgc tggttgactg gctgagctac tcacagctga tgcccgagtc
     1381 ctcgctggtg atctgtcacg gcggccacgg caccgtcgcg cgggcccttg cggcaggggt
     1441 gccggtactg acctgcccgg cagccgggga catgaacgag acggccgccc gggtcacctg
     1501 ggccgaggtc ggccgctcga tccgctggag tctcaccggg ccgagaacct tgcgctgggc
     1561 ggttgctgaa attctgaatg agcccctgta ccgggcccga gcggggcaga tttccgcctg
     1621 ggatgccgag cacgacggtg ccgtccgggg agcggagctg gtcgaagctc tggcggaccc
     1681 cggaagaaac tgagcccgct ccggttggcg ggcgggctca agcaactcgg gggggcggac
     1741 tcgaaccgcc aaccctctga ttaacagtca gatgctcta
//
LOCUS       DAOQKN010000313         1736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_60117_length_1736_cov_3.765021, whole genome shotgun sequence.
ACCESSION   DAOQKN010000313 DAOQKN010000000
VERSION     DAOQKN010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60117_length_1736_cov_3.765021"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            225..>1736
                     /locus_tag="PLJ64_12855"
     CDS             225..>1736
                     /locus_tag="PLJ64_12855"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNC94420.1"
                     /translation="MTESLADGFDPVPFDEWSDAATRGDENLALMTLLENGVEAKWLY
                     TPEDAIAADPSGLPGQAPFTRGTRAGRHWQIRQEQTNPELTKANREILEDLNGGVTEI
                     TLRFGDDGIPITNLDQLSEVLDGVYLDLARVALEAGASAPAAAGLLKKLWRENGISPD
                     VALGSFRYDPLGTLARTGTLPSSPEQALEAAASLAADTAREWPKARALAVDTGIYVEA
                     GATAGWELGIAIATGVEYLRAAAAAGLEPADAAAQIEFTLAVGPDQFLEMAKFRAIRR
                     LWARVLEESGVEEAARFSPTYGTTSARMITAVDPWVNMLRATTAAFAAGTGGADGVTV
                     TPFDRMIGQPGDLGRRIARNTQIILQDESSLGRIADPLAGSWYGEELTDEVAKAGWER
                     FREIEREGGALAALRSGLIATTLAGLADEREDDLNHRRRVMTGINEFPLLGDDGTDPQ
                     KVDRPIGSGDPLAELEGAVIGETFSLPALNPRHDSTPFEKMRRSAQEHEEKGGD"
BASE COUNT          306 a          579 c          577 g          274 t
ORIGIN      
        1 atgctcgcca gggcgaaacg ctggatgatt gccctgaggg gatcctgctt gacccggatc
       61 agacggaacc gggtcggggc gatcggcagc ggcggtggct gtttgcgctc ccgtttcaag
      121 tgggagcagc atagaacctg caacagccat ttccctaccg gcgaggcgga aaccgggctc
      181 ggacaggtac cctgcaaggc ccgtgtttac ctctctggag tgccatgact gagtccctcg
      241 cagacggttt cgaccccgtt ccattcgatg aatggagcga cgccgcgacc aggggcgacg
      301 agaaccttgc cctgatgacc ctgctcgaaa acggggtcga ggcgaaatgg ctctacaccc
      361 cggaggatgc gatcgccgcc gacccgtcgg gtctgccggg acaggcacca ttcacccgcg
      421 gcacacgggc tggccgacac tggcagatcc gccaggagca gacgaacccc gaactgacca
      481 aggccaacag ggaaattctc gaggatctga acggcggggt caccgaaatc accctccgtt
      541 tcggagatga cggaattccg atcacgaacc tcgatcagct gagcgaggtc ctggacgggg
      601 tctacctgga tctggcccgc gtcgcgctgg aggccggtgc ctcggcaccc gcagccgccg
      661 ggctcctgaa gaagctctgg cgggagaacg ggatctcgcc ggacgtcgcg ctcggttcgt
      721 tccgttacga cccgctcggc accctcgccc ggaccggcac cctgccaagt tcaccggagc
      781 aggcactcga ggcggccgcc tcattggccg ccgataccgc ccgcgaatgg ccgaaggcca
      841 gggcgctggc ggttgacacc gggatttatg tcgaggcggg agccacggcc ggctgggagc
      901 tcggcatcgc gatagcgact ggagtcgagt acctccgcgc cgccgcggcc gccgggcttg
      961 agccggccga tgcggctgcc cagatcgagt tcacgcttgc ggttggtccg gaccagttcc
     1021 tggagatggc caagttccgg gcgatccgcc ggctctgggc ccgggtgctc gaagagtccg
     1081 gggtcgagga agcggcccgc ttctccccga cctacggaac cacctccgcc cgcatgatca
     1141 ccgcggtcga cccctgggtc aacatgcttc gcgcgaccac cgccgctttc gcggctggca
     1201 ccggcggtgc cgacggagtc accgttacgc ccttcgacag gatgattggc cagccgggtg
     1261 acctcggccg ccgcatcgcc cgtaacaccc agatcatcct ccaggatgaa tcttcgctcg
     1321 gccggattgc cgatccgctg gccggctcct ggtatggcga ggaactcacg gatgaggtcg
     1381 cgaaggcggg ttgggaacgc ttccgcgaga tcgagcggga aggcggggcg ctcgccgcgc
     1441 tccgttcggg tctgattgcc accaccctgg ccggacttgc cgacgaacgc gaagatgatc
     1501 tcaaccatcg tcgccgcgtc atgacgggaa tcaacgagtt cccgttgctg ggtgatgacg
     1561 gaaccgaccc gcagaaggtt gatcgaccaa ttggctccgg cgatccgctg gctgagctcg
     1621 agggcgcggt catcggcgag accttctccc tgccggcgct caacccgcgt cacgactcga
     1681 cccccttcga gaaaatgcgc cggtccgcac aggagcatga ggagaagggc ggcgac
//
LOCUS       DAOQKN010000314         1735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_60167_length_1735_cov_5.190476, whole genome shotgun sequence.
ACCESSION   DAOQKN010000314 DAOQKN010000000
VERSION     DAOQKN010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60167_length_1735_cov_5.190476"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="PLJ64_12860"
     CDS             complement(<1..421)
                     /locus_tag="PLJ64_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94421.1"
                     /translation="MNSNRTRLLALLAVVVVAAGLFLVLKPSDDKKDDSTSSAGTEKV
                     ASSTEQTVEAKTEKPKPDIPEVSFENGEPAGGELKVEASKGDTIAFKVRSDVSEEIHV
                     HGYDISREVGPGKPVTLRFPADLVRIYEVEMEGGGIPI"
     gene            complement(447..1679)
                     /locus_tag="PLJ64_12865"
     CDS             complement(447..1679)
                     /locus_tag="PLJ64_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC94422.1"
                     /translation="MPTPSILLPLRNPIYRKLFSAQVIALLGTGLTTIALGLLAFDLA
                     EDDAGQVLGVALALKMASYVVVAPLVAAWAESRSRKNLLVGLDVIRALAVVSIAFVNQ
                     VWEVYLLIVVLSSASAGFTPAFQAVIPDIFEDVEKYTEALSLSRLAYELEALLSPALA
                     AALLGVLSYSGLFAANAAAFVCSAILVLTTAIPAAGAGIARSQRGFARVTSGIRQYLT
                     VPRLRGLFALNLAAASASAMVVVNTVIYVKSDFSLDDSDVAWALGIVGAGSLLVAITL
                     PWVLRRTTDRLLMIRGGLVLALGLVAATLIESFAALLLVWAALGVGLALVQTPAGRLI
                     QRSGNPESRPGLFAAQFSLSHFCWFVTYPVAGFAGAAFGLHITAMILAVLAGASTLLA
                     AALWRSPSHLPAQPAGNT"
BASE COUNT          376 a          497 c          562 g          300 t
ORIGIN      
        1 caatcgggat gccgccaccc tccatctcaa cctcgtagat ccgcacgagg tcggcgggga
       61 accggagggt cacgggcttg ccgggtccta cctcgcgact gatgtcgtaa ccatggacgt
      121 ggatctcttc gctcacgtcc gaccggacct tgaatgcgat cgtgtcgccc ttcgaggctt
      181 caaccttgag ttctcccccc gcgggctcac cattctcgaa gctgacttca gggatgtccg
      241 gcttgggctt ctcggtcttt gcctcgacgg tctgttcggt cgaagaagcg accttctcgg
      301 tgccggcgct actcgtcgaa tcgtccttct tgtcatccga tggctttagg accaggaaga
      361 ggccagcggc tacgacgact acggcaagga gggcaagaag gcgggtgcgg ttcgagttca
      421 taagtgcaaa atatatgtcg agatgttcat gtgttgcctg ccggttgcgc aggaagatga
      481 gagggggagc gccagagagc cgcggcgagg agagtcgagg cgccggctag gacggcgaga
      541 atcatggccg tgatgtggag tccgaatgcg gcacccgcga agccggcgac cggataggtc
      601 acaaaccagc agaagtgtga aagggagaac tgggcggcga aaagcccggg acgactctcg
      661 ggatttccgg agcgctggat tagacgtcca gcaggcgttt gcaccaaggc gaggccaact
      721 ccgagtgccg cccagacgag caggagggca gcgaaggact cgatcagcgt ggctgcgact
      781 agaccgaggg cgaggaccaa gccgccacgg atcatcagca ggcgatccgt tgttctgcgg
      841 aggacccacg gaagggttat cgcgaccagg agggaccccg cgcccacgat gccaagtgcc
      901 cacgcaacat ccgagtcgtc gaggctgaag tccgacttca cgtagatgac cgtgttgacc
      961 acgaccatcg cactcgccga cgcggccgcg aggttgagtg caaacagtcc gcggagtctc
     1021 ggcacggtca agtattggcg gatgcctgac gtgacccggg caaagccgcg ctgggatctc
     1081 gcgatacctg cgcccgcggc ggggatcgcg gtggtcaata cgaggatcgc cgagcacacg
     1141 aatgccgcgg cgttggcggc gaacaatccg gaataagaaa ggactccgag aagcgctgcc
     1201 gctagagctg ggcttaggag cgcctcgagt tcataggcga gcctggacag cgagagtgct
     1261 tccgtgtact tctccacgtc ctcgaagatg tctggaatga ctgcctggaa agctggggtg
     1321 aatccggccg atgcggagct caaaacgacg atgagcagat agacctccca cacctggttg
     1381 acgaacgcga tcgagacgac ggccagagct ctgatcacgt ctagtcctac caacaggttc
     1441 tttcgcgacc ggctctcggc ccaagcggca accagtggcg ccaccacaac gtacgaggcc
     1501 attttcaatg cgagggcgac gcccagcacc tggcccgcgt cgtcttcggc gagatcaaac
     1561 gcgaggagtc ccagagcgat cgtcgtcagc ccggttccga gcagagcgat cacctgggct
     1621 gagaacagct ttcggtagat cggattccgg agaggaagaa ggatcgaagg cgttggcatt
     1681 gaactgagta tatgaagaac tattcatgta tgtacgccaa tgccctagaa tctct
//
LOCUS       DAOQKN010000315         1733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_60304_length_1733_cov_3.815256, whole genome shotgun sequence.
ACCESSION   DAOQKN010000315 DAOQKN010000000
VERSION     DAOQKN010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60304_length_1733_cov_3.815256"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            197..418
                     /locus_tag="PLJ64_12870"
     CDS             197..418
                     /locus_tag="PLJ64_12870"
                     /inference="COORDINATES: protein motif:HMM:NF021378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HNC94423.1"
                     /translation="MSNRVSRRQANKWAAQDAQQAPQPQYVEPAAPAPAAAPAADPIA
                     QLKELGELHQSGVLTDEEFAAQKAKILGS"
     gene            430..861
                     /locus_tag="PLJ64_12875"
     CDS             430..861
                     /locus_tag="PLJ64_12875"
                     /inference="COORDINATES: protein motif:HMM:NF018021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HNC94424.1"
                     /translation="MTDDEQSAPGVCPPGRASVFTRGTGKHGQHKHHEHPHSVSDEEF
                     EALIIRAIDRLPEQFREIINQVPVVISDQGRERHAYGLYQGDGIARDNYPDRIIIFKD
                     TLVRDFGHDPALLAAQVERTVRHELGHHLGYDEGGVRGLGL"
     gene            871..1326
                     /locus_tag="PLJ64_12880"
     CDS             871..1326
                     /locus_tag="PLJ64_12880"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002525071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNC94425.1"
                     /translation="MSKATLNTNAGPITIEFFDDDAPKTVENFRKLAADGFYDGLTFH
                     RVIKDFMIQGGCPLGTGTGGPGYTFEDEFNDHGVVRGALAMANAGPNTNGSQFFIVTI
                     PSADWLNGKHTVFGEVTDGMDVVDAIEGTQTDGRDMPLDPQVIESVELA"
BASE COUNT          329 a          572 c          538 g          294 t
ORIGIN      
        1 ggctggcccc ttcgccgccg ccgtgcgtcg ttcgggcggc cagctcgttg ccagtggacg
       61 catcccggtc caggacctgc tcgccagcct cgacgctgcc gaagcgtagg aacttcagag
      121 aaagaaggag aacaaaatgc caggattgat cagaggtgta gcccgaaccg cggtcatcgc
      181 cggtacggcg acaaccgtga gtaaccgtgt ttcacgccgc caggccaaca aatgggccgc
      241 gcaggatgcg caacaggcac cgcagcccca atacgtggaa ccggctgctc cagctccggc
      301 agcggccccc gctgctgacc cgatcgccca gctcaaggaa ctcggtgaac tccaccagag
      361 cggcgtgctg accgacgagg aattcgccgc gcaaaaggca aagatcctcg gcagttgagg
      421 tggcaggcgt tgaccgatga cgaacagtcc gccccgggag tctgtcctcc cgggcgggcc
      481 tccgtcttca cccgtggtac cggcaaacac ggtcagcaca agcatcacga gcatccccat
      541 tcggtctcgg acgaagagtt cgaggcgttg atcatccggg cgatcgaccg gctgcccgaa
      601 cagttccgcg agatcatcaa ccaggtaccg gtggtgatct ccgaccaggg tcgcgaacgg
      661 catgcctacg gcctctatca gggtgacgga atcgcccggg acaactaccc cgaccggatc
      721 atcatcttca aggacaccct ggtccgggac ttcggccacg accccgccct gctggccgca
      781 caggtcgaac gaacggtccg gcatgagctt ggtcatcacc tcggatatga cgagggtggc
      841 gtccgaggcc ttggcctgta ggctcgcagc atgtcgaaag caacgctgaa taccaacgcc
      901 ggtccgatca ccatcgagtt cttcgatgac gacgccccca aaacggtcga gaacttccgc
      961 aaactcgccg ctgacggttt ctatgacgga ctcaccttcc accgggtgat caaggacttc
     1021 atgatccagg gcggctgccc gctcggtacc ggtactggtg gccccggcta caccttcgaa
     1081 gacgaattca atgaccacgg agtggtgcgc ggcgcgctcg cgatggctaa cgccggcccc
     1141 aacaccaatg gttcccagtt cttcatcgtc accatcccct ccgccgactg gctgaacggc
     1201 aagcacacgg tcttcggcga ggtcaccgac ggtatggacg tggtcgacgc gatcgaagga
     1261 acccagaccg acggccgcga catgcccctg gatccccagg taatcgagag cgtcgagctc
     1321 gcttaactgc agtcgcgccg gtctgtggcg taagtgggat ctcgcggtcc tggaaccggg
     1381 ttgacgtagc cgtgggggct ggtatgcatc tccgggacac tcaggagtag ggcggctgaa
     1441 tgccgcccgc atcccgcgtc ccggggatgc atgccagccc ccacggcgta actaccgagg
     1501 ctaggtcccg gcgatgctca cccaaccccg cggcgcaacc accaacgccg ggatgatcag
     1561 gctttggtgg gttcttcagc ctcttcgggg gcggtctggt cgatgtagat gacctggtcg
     1621 ccggcccgga gggtctggca ttcctcgtca ccggggcggt ggaccttctg gtcgcggatc
     1681 acggcgatcg ccgtctgatg ggggcgttgc tcgatctgtt cgccgtcggt ttc
//
LOCUS       DAOQKN010000316         1721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_61082_length_1721_cov_4.582233, whole genome shotgun sequence.
ACCESSION   DAOQKN010000316 DAOQKN010000000
VERSION     DAOQKN010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61082_length_1721_cov_4.582233"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="PLJ64_12885"
     CDS             <1..620
                     /locus_tag="PLJ64_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HNC94426.1"
                     /translation="ARRIVENTLHIDLPARMKVATRQVGRRPAIVCGTGYTGEDGVEL
                     LIDPEIAPAIWTELVDSGVVPCGLGARDTLRLEVCFHLHGNDLTTETDPISAGLGWAC
                     VESTGFIGSDKVAVFRANGTDEKLAPFVIEGAGIPRQGNPVMLGEKQVGEVTSGTFSP
                     SLEQGIGMAYLRTDLAEPGTEVEVDVRGKRRTARVSSKPLYKKER"
     gene            622..1008
                     /gene="gcvH"
                     /locus_tag="PLJ64_12890"
     CDS             622..1008
                     /gene="gcvH"
                     /locus_tag="PLJ64_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNC94427.1"
                     /translation="MTEASYPEDLKYHPEHDWARIEGGEATLGITWYAQDSLGEVVFF
                     EGPEIGSEVTKDQPYAEVESVKAVSDVIAPVSGEVIAVNDSLGDAPEAINADPYETGW
                     LIKVRLSDPSEADSLMDAAAYAASLE"
     gene            1015..>1721
                     /locus_tag="PLJ64_12895"
     CDS             1015..>1721
                     /locus_tag="PLJ64_12895"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF014408.2"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 2 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HNC94428.1"
                     /translation="MAGYTPATDPERAEMLARIGVDSIDDLFAQIPEEVRLGRPLDLA
                     DGLSESEVFDRLDTMAMQNFGGSGTTFLGAGMYDHYVPAIVEAIISRSEFLTPYTPYQ
                     PEVSQGGLQVMFEFQTAMSEITGLPVSNAGLYEGPSAAASAAYLAMGQTKRTGLVASR
                     GLHPHSRETLATHAVGFGATLTEVGLDGGLTSGEELEAAITEDTAAVFLQNPNFLGAV
                     EDIAALGEAVKARGALL"
BASE COUNT          326 a          568 c          544 g          283 t
ORIGIN      
        1 atgcccgacg gatcgtcgag aacacccttc acatcgatct gcccgcccgg atgaaggtcg
       61 ccacccgcca agtcggccgt cgaccggcga tcgtttgcgg caccggttac accggcgaag
      121 acggggtcga attgctgatc gacccggaaa ttgccccggc gatctggacc gaactggtcg
      181 attccggcgt cgttccctgc ggcctcggcg cccgcgacac tctgcggctc gaggtctgct
      241 tccaccttca cggcaacgac ctgaccacgg aaaccgatcc gatttcggcc ggactcggct
      301 gggcatgtgt cgagtcgacc ggcttcatcg gttccgacaa ggtggccgtc ttccgcgcca
      361 acggaaccga cgagaagctc gccccgttcg tgatcgaggg ggccgggatt ccgcgacagg
      421 gcaacccggt catgctgggc gaaaaacagg tcggcgaggt gaccagcggc actttctcgc
      481 cttcgctgga gcagggcatc ggcatggcat acttgcggac cgatctggcc gaacctggta
      541 ccgaagtcga ggtcgacgtg cgtggaaagc gtcggacggc ccgcgtaagc tccaaacccc
      601 tttacaagaa ggagcgatga attgactgag gcgagctacc ccgaagacct gaaataccac
      661 cccgagcacg actgggcgag gatcgaaggc ggcgaggcca ccctcggaat cacctggtac
      721 gcccaggact cgctcggcga ggttgttttc ttcgagggtc ccgagatcgg ctcggaagtg
      781 accaaggatc agccctacgc ggaagtcgaa tcggtcaagg ccgtctccga cgtgatagcg
      841 ccggtctccg gcgaagtgat cgctgtgaac gacagcctgg gtgatgcccc ggaagcaatc
      901 aacgccgatc cctacgagac cggctggctg atcaaggttc gtctcagcga tccgtccgaa
      961 gcagattcac tgatggacgc ggcggcctac gccgctagcc tggagtagcc aaccttggcg
     1021 gggtacacac cggctacaga tcccgagcgg gccgagatgc tggcccggat cggggtggac
     1081 tcgatcgatg atctgttcgc gcagatcccc gaggaggtgc ggctgggccg gccgctcgac
     1141 ctcgccgacg gtctcagcga atccgaagtc ttcgatcgcc tcgacacgat ggcgatgcag
     1201 aacttcggcg gcagcggcac caccttcctt ggggccggga tgtacgacca ctacgtgccc
     1261 gcgatcgttg aagcaatcat cagccgctcc gagttcctca ccccttacac gccgtaccag
     1321 cccgaggttt cgcagggcgg ccttcaggtg atgttcgagt tccagaccgc gatgagcgag
     1381 atcaccggcc tgccggtctc gaacgccggt ctctacgaag gaccttccgc cgccgcctcg
     1441 gccgcctacc tggcgatggg gcagaccaaa cggaccgggc tggtcgcttc gcgcggccta
     1501 cacccgcaca gccgcgaaac cctggccacc cacgcggtcg gtttcggcgc caccctgacc
     1561 gaggtcggtc tcgatggcgg attgaccagc ggcgaggaac tcgaagccgc gatcaccgaa
     1621 gacacggcgg ccgtcttcct ccagaaccct aatttcctcg gtgcggtcga agacatcgcc
     1681 gcgctcggtg aagcagtcaa ggccagggga gccctgctga t
//
LOCUS       DAOQKN010000317         1718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_61304_length_1718_cov_5.042093, whole genome shotgun sequence.
ACCESSION   DAOQKN010000317 DAOQKN010000000
VERSION     DAOQKN010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61304_length_1718_cov_5.042093"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..906
                     /locus_tag="PLJ64_12900"
     CDS             133..906
                     /locus_tag="PLJ64_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNC94429.1"
                     /translation="MSFCVDGEAKAIKASATWGQYKREKRDDQQDYKGYPLKVVMPDN
                     VTAERSELLEMYGAEIVYSEGAKGSNGAVELALEMAEDPQYYMPYQYGNEANPNAHYD
                     GTAVEILEELDEVTAFVAGLGTGGTLMGNGRRLKEANPETRIVAAEPMQGELVQGLRS
                     LDDGFIPPIIDLSLLDRKIMVSNMDAIIWTKKLLSQEGIFAGVSSGAVAAIAVRIANE
                     IDSGNIVFLIPDGGWKYLSSGVYTKSIEELGDIDATNWW"
     gene            917..1315
                     /locus_tag="PLJ64_12905"
     CDS             917..1315
                     /locus_tag="PLJ64_12905"
                     /inference="COORDINATES: protein motif:HMM:NF025819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HNC94430.1"
                     /translation="MKVPQSLLDEMVEHSKADAPDECCGLIGGAGDEAITLHRMENTA
                     HSPLRYEMDSKEQLKVYKQILEAGHDVVGIYHSHTRTAAYPSQTDINLASGWPDAVYF
                     IVSLENPDEPYVRGFNIREGQVEDVDIQAV"
     gene            1293..1571
                     /locus_tag="PLJ64_12910"
     CDS             1293..1571
                     /locus_tag="PLJ64_12910"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HNC94431.1"
                     /translation="MSIFRRSDSTRSGKTPEPVKVAWANHQAEAELIAGILREEGIPS
                     LIKRNKGFDVPDFLAAGARDIFVPASAADRAREILDDLKSDEDPLPGS"
BASE COUNT          352 a          571 c          530 g          265 t
ORIGIN      
        1 gatgaggaag aagacgaaga gattccgctc atccccgacg aactctcgga aggcggaagc
       61 gacaccgccg atgacggcac aactgacgcc gatactcccg tcgttcaagc ccacttgcca
      121 tcgtcatttg gcatgagctt ctgcgttgat ggagaagcca aggcgatcaa ggccagcgcc
      181 acttggggcc agtacaagcg ggagaaacga gacgatcaac aggattacaa gggctacccg
      241 ctcaaggtgg tcatgcccga caacgtcacc gccgagcgca gcgagctgct cgagatgtac
      301 ggcgccgaaa tcgtctactc ggaaggcgcc aagggttcca acggagcggt cgaacttgcc
      361 ctggagatgg ccgaggaccc gcagtactac atgccttacc agtacggcaa cgaggccaat
      421 ccgaacgcac attacgacgg caccgcggtc gagatcctcg aggagctcga cgaggtgacc
      481 gccttcgtgg ccgggctcgg cacgggcggc accctgatgg gcaacggccg tcgcttgaag
      541 gaagcgaacc cggagaccag gatcgtcgcg gccgagccga tgcagggcga gctggtccag
      601 ggcctccgct ccctcgatga cggcttcatc ccgccgatca tcgacctttc cctgctcgac
      661 cggaagatca tggtctcgaa catggacgcg atcatctgga cgaagaagct gctttcccag
      721 gaaggaatct tcgccggggt ttcttccggt gcggtcgccg cgatcgcggt acggatcgcg
      781 aacgagatcg actcgggcaa catcgtcttc ctgatccccg acggcggctg gaagtacctc
      841 tcctccggcg tctacacgaa gtcgatcgag gaactcggcg acatcgacgc gaccaactgg
      901 tggtaggccc gctccgatga aagttcccca gtccctgctc gacgagatgg tcgagcacag
      961 caaggctgat gctccggacg aatgctgcgg cctgatcggc ggcgccggcg acgaggcgat
     1021 cactctccac cggatggaga acaccgccca ctccccgctc cgctacgaga tggattcgaa
     1081 ggagcagctc aaggtctaca agcagatcct cgaagccggc cacgacgtgg tcgggatcta
     1141 ccactcccac acccggaccg cggcgtaccc ctcgcagact gacatcaacc tcgccagcgg
     1201 atggcccgac gccgtctact tcatcgtctc gctggaaaac ccggacgagc cgtatgtccg
     1261 gggcttcaac atccgcgagg ggcaggtcga ggatgtcgat attcaggcgg tctgattcca
     1321 cgcgttcggg caagacgccc gagccggtca aggtcgcctg ggccaaccat caggccgaag
     1381 ccgaactgat cgcgggaatc ctccgggagg agggcatccc ctcgctgatc aaacgcaaca
     1441 agggattcga cgttccggac ttcctcgccg ccggcgcccg cgacatcttc gtccccgcct
     1501 ccgccgcgga ccgcgccagg gagatcctcg acgacctcaa gtcagacgaa gaccccctgc
     1561 ccggcagtta ggggccggag cccggtgtac cggcctcctc ggcccgaaac gtcgtcggcc
     1621 ggcacaccgg accccggcca ccggatttgg ttcaggccgg ggaaccgccg gccatggccg
     1681 ggaggatcat cacggtgtcg gtgtcgctga ccccggtt
//
LOCUS       DAOQKN010000318         1709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_61940_length_1709_cov_3.619105, whole genome shotgun sequence.
ACCESSION   DAOQKN010000318 DAOQKN010000000
VERSION     DAOQKN010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61940_length_1709_cov_3.619105"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            337..1035
                     /locus_tag="PLJ64_12915"
     CDS             337..1035
                     /locus_tag="PLJ64_12915"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC94432.1"
                     /translation="MTRLAAGRSTHLEPEEIAAETLRLFDRGDDPSIRQLATALSVTP
                     SAIYHHFESRSEIVEAAVELVWIEILTNVAEDVGNTFEADPEEVLVAVGLHTRRAFIL
                     HHTIAPYMTVTPRAKNLQAGGIALFANAFERLGVTGEAAGDAFHHYGSYCFGTALYAA
                     SRLNARAALGPDRSDHGELETLREEAGVTGTSDEKTRAALDRVLDLSEIDPARDEHLF
                     VLGLKQILKGFARV"
     gene            1039..>1709
                     /locus_tag="PLJ64_12920"
     CDS             1039..>1709
                     /locus_tag="PLJ64_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNC94433.1"
                     /translation="MSLVRTERDGPMAVITLNSPPLNLFDRAMTEELMAAFEAIQNDP
                     PRGLLVRAEGEVVSGGVNVNEFKGLTAAEGTRLWEQLLGLIDRVEGFPFPVVFAAHGL
                     TLTAAFELSLACDLIIASESAHFGLVEIVVGLTPSMGGPQRLAERAGSGRARQLVMTG
                     GLFDAATLERWNVVNEVKPDEEIDAYARKLTKRLSNGPTLAHAMTKRIVRAWRDEGMA
                     GADRVT"
BASE COUNT          308 a          587 c          533 g          281 t
ORIGIN      
        1 gcccgcagcc tcaacatcca cccgcagacg gcccggtacc ggatcgcccg gctgagggag
       61 ttcttcggcg acgacctcga ccggcccgat cgccgcctcg ccctcgaact ctcgctgcgc
      121 acccgctgac ccccggttcc catctgggtt gaagttattc gcacatggaa cggataactt
      181 caacccagat ggagtcagcc ggaccggccg ggcgagatta cggggtttcc cgaagttttc
      241 gtcgaaatat tgctgttaga ttccgtgcgc tgttcggtta tgccggtcag ccgcccgaga
      301 acctggagag acgggcgcac caaaaggagg catgttttga cccgattggc agcaggacgc
      361 agcacgcatc tggagccgga agagatcgcg gcagaaactc ttcggctgtt tgaccgcggg
      421 gatgacccca gcatccggca actcgccacc gccctcagcg tcaccccttc cgcgatctac
      481 caccacttcg aatcccggtc cgaaatcgtc gaggccgcgg tcgagctggt ctggatcgag
      541 atcctcacca acgtggcaga ggacgtcggc aacaccttcg aggccgatcc ggaggaggtc
      601 ctggttgcgg tcggccttca tacccgccgg gccttcatcc ttcaccacac gatcgctccg
      661 tacatgacgg tcactccccg cgcaaagaac cttcaggccg gcggcatcgc cctcttcgcc
      721 aacgccttcg agaggctcgg ggttaccggc gaggcggccg gagacgcctt ccaccattac
      781 gggagctact gcttcggcac cgcgctttac gcggccagcc gactcaacgc ccgggccgcc
      841 ctgggacccg acagatccga ccacggcgaa ttggaaaccc tgcgcgagga agccggggta
      901 accggcactt cggacgagaa gacccgggcc gcgctcgacc gggtgctcga cctttccgag
      961 atcgatcccg cccgggacga gcatctcttc gtactgggct tgaagcagat tctcaaggga
     1021 ttcgccagag tctgaggcat gtcactagtc agaacagagc gcgacgggcc gatggccgtg
     1081 atcacgctga acagtccgcc gctgaacctc ttcgaccggg cgatgaccga agaactcatg
     1141 gccgccttcg aggcgatcca gaacgacccg ccccgcggtc tcctggtccg ggccgagggt
     1201 gaagtcgtct ctggcggggt caacgtgaac gagttcaagg gcctgaccgc cgccgagggc
     1261 acccggctct gggaacagct gctggggctg atcgaccggg tcgagggctt ccccttcccg
     1321 gtcgtcttcg ccgcccacgg tctgaccctg accgcggcct tcgagctctc gctggcctgt
     1381 gacctgatca tcgcctctga atcggcccac tttggcctgg tcgagatcgt ggtcggcctg
     1441 accccctcga tgggtggccc gcagcggctg gccgaaaggg ccggctcggg ccgcgcccgg
     1501 cagctggtga tgaccggagg cctctttgac gcggcgaccc tcgaacgctg gaacgtggtc
     1561 aacgaagtca agccggacga ggaaatcgat gcctacgccc gcaagctgac caaacgcctg
     1621 tccaacgggc cgaccctcgc ccacgcgatg accaagcgga tcgtccgggc ctggcgggat
     1681 gaaggaatgg ccggcgccga ccgggtgac
//
LOCUS       DAOQKN010000319         1697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_62765_length_1697_cov_6.792326, whole genome shotgun sequence.
ACCESSION   DAOQKN010000319 DAOQKN010000000
VERSION     DAOQKN010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62765_length_1697_cov_6.792326"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="PLJ64_12925"
     CDS             <1..283
                     /locus_tag="PLJ64_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94434.1"
                     /translation="AAILVLAAGATWYAVSQSWFLGTDPAGRVALYSGLPYELPFGIE
                     LYKLKYSTAIQTGSLPESRQRSVTDNEVRSKDDAVSLIEDIESREGLTG"
     gene            280..1596
                     /locus_tag="PLJ64_12930"
     CDS             280..1596
                     /locus_tag="PLJ64_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNC94435.1"
                     /translation="MSASRNRELLALVPVALLLTVGFAAVFAQNDNQLTNLSLSYGLY
                     FLAICLATHIYIRIRLPNADPYLFPLMALLTAIGLVMLYRLDSELGNELARDQANWFV
                     IGLILFALVIHFFKDYSVLERYRYTIAFISLALLMAPRLPLIGSQVNGAYLGISFGPI
                     SFQPAELAKIGIVIFLASYLREHRELLVVGAKRFMGITFPPLKHLGPLLVVWGAAMFM
                     LIFIRDLGSSLMFFAAFLALIYVATGRLSFVVIGMAMFLAGAWVIYHTVPHVTDRVDI
                     WLDPYQDPSGSGYQILQSMFAMADGGLFGEGFAQAMVVIPGTDAPLLPAAQTDLIYTL
                     ITSELGLFGACAVIATYLLIAARGFKVALMANDGFSKLLATGLTTVFAFQAFVIIGGV
                     TRVIPLTGVTLPLVSYGGSSILANFILLALLLLVSDRARREAREGI"
BASE COUNT          241 a          602 c          503 g          351 t
ORIGIN      
        1 ggcagcgatc ctcgtgctcg cggccggcgc gacctggtac gcggtcagcc agtcctggtt
       61 cctcggcacc gacccggccg gccgggtcgc cctctacagc gggctaccct acgagctgcc
      121 cttcgggatc gagctctaca agctcaagta ctcgaccgcg atccagaccg gttcactgcc
      181 ggagagccgt cagcgctcgg tcaccgacaa cgaggtccgc tccaaggatg acgcggtttc
      241 gctgatcgaa gacatcgaaa gccgtgaggg cctgaccggg tgagtgcctc ccgcaaccgg
      301 gaactgctgg ccctcgtccc ggttgccctg ctcctgaccg tgggcttcgc cgcggtcttc
      361 gcgcagaacg acaaccagct gaccaatctg agcctctcct acgggctcta cttcctggcg
      421 atctgcctcg ccacccacat ctacatccgg atccgcctgc ccaacgcgga cccttacctc
      481 ttcccgctga tggccctgct cacggcgatc ggcctggtca tgctttaccg gctcgattcc
      541 gagctcggca acgagctcgc ccgggaccag gccaactggt tcgtgatcgg gctgatcctc
      601 ttcgccctgg tcatccactt cttcaaggac tactcggtcc tggagcgcta ccggtacacg
      661 atcgccttca tcagtctcgc cctcttgatg gcgccgcggc tgccgctgat cggctcccag
      721 gtcaacggcg cttacctggg aatcagcttc gggccgatct ccttccagcc ggccgagctg
      781 gccaagatcg ggatcgtcat cttcctcgcc agttacctgc gtgagcaccg cgagctgctg
      841 gtggtcgggg cgaagcgttt catgggcatc accttcccgc cgctcaagca cctcgggccg
      901 ctgctggtgg tctggggcgc agcgatgttc atgctgatct tcatccgcga cctcggcagt
      961 tcgctgatgt tcttcgccgc cttcctggcc ctgatctacg tggcgaccgg gcggctctcc
     1021 ttcgtggtga tcggcatggc gatgttcctc gccggggcct gggtgatcta ccacacggtt
     1081 ccccatgtaa ccgaccgggt cgacatctgg ctcgacccgt accaggaccc ttccggctcc
     1141 gggtaccaga tcctgcagtc gatgttcgcg atggcggacg gagggttgtt cggcgagggc
     1201 ttcgcccagg cgatggtggt cataccgggg acggatgcac cactcctgcc ggctgcgcag
     1261 accgacctga tctacaccct gatcacctcc gagctgggtc tgttcggcgc ctgcgccgta
     1321 atcgccacct acctgctgat cgcggcccgc ggtttcaagg tcgcgctgat ggccaacgac
     1381 ggcttctcga agctgctcgc caccgggctg accaccgtct tcgccttcca ggccttcgtg
     1441 atcatcggcg gcgtgacccg ggtgatcccc ctgaccggcg tcaccctgcc gctggtttcg
     1501 tacggcggtt cctcgatcct cgccaacttc atcctgctgg cgctgctgct gctggtttcc
     1561 gaccgggccc gccgcgaagc ccgcgaaggc atctgaggac ggccggaatc gatgaacgca
     1621 cggatcgtca ggctgttcgc tttcatcagc gtgatgttcg ccgtgctggt cggtttcacc
     1681 gcctactgga cggtctt
//
LOCUS       DAOQKN010000320         1697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_62766_length_1697_cov_5.667479, whole genome shotgun sequence.
ACCESSION   DAOQKN010000320 DAOQKN010000000
VERSION     DAOQKN010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62766_length_1697_cov_5.667479"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1697)
                     /locus_tag="PLJ64_12935"
     CDS             complement(<1..>1697)
                     /locus_tag="PLJ64_12935"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HNC94436.1"
                     /translation="LYEGPRDPEGLLAHMDYDSFRPGQREAVEAALQGRDSLVVMPTG
                     GGKSLCYQLPGLASPSLTIVVSPLIALMADQVKRLRSEGHPAVMFASGLGDEDAAASW
                     QAVKDGSARIAYCSPERFGSAAFLDLIGSREIDLLAIDEAHCLSEWGHDFRPDYLRLP
                     KVAERLGRPAVMACTATATPQVVAEIAERMGMNDPAEIHSGFDRPNLSFDTIPLEGKG
                     SKARKQALLELGLADPANRPAIVYCGTRKSTEELAEDLRSAGLSAVGYHAGMAPDERA
                     SAQHRFMEGDAEVVVATNAFGMGIDKANVRSVWHWAIPSSVEAYYQEAGRAGRDGEPA
                     RAVLLAMRADLGRLVRFNEQRKVDPSDVLSYVDDVRRAAGHDADGTPGSVAIEAPRSD
                     EERIRIAVAERAGALKVDPAPGGRLLITPGPESGQGPIRAACRIAEDRGWVAYRAVEA
                     FSFSSTCRRRQLLDHFGDQSPATPTGRCCDICDPIEWLPDPDSIEARKSRSTSRKSAG
                     PPPELAPQDEALYEDLRRWRLEAAGDRPAYHVASNRTLTAIATVKPLDEYSLMGVAG"
BASE COUNT          257 a          563 c          599 g          278 t
ORIGIN      
        1 ccccgccacg cccatcagtg agtactcgtc aagcggcttc acggtggcga tcgcggtgag
       61 ggtgcggttc gaagcgacgt gataggcggg gcgatctccc gctgcctcga gccgccagcg
      121 gcggaggtcc tcgtacaggg cttcgtcctg gggggcgagc tcgggcggcg gtccggcgga
      181 cttgcgggaa gtcgatcggg actttcgagc ctcgatcgag tccgggtcgg gcagccactc
      241 gatgggatca catatgtcac aacaacggcc ggtcggagtt gccgggctct gatccccgaa
      301 atggtcgagc agctggcgtc ggcggcaggt gctggagaag ctgaacgctt cgaccgcacg
      361 gtaggcgacc cagccgcgat cttcggcgat ccggcaggcg gcccggatcg gaccctggcc
      421 tgattccggc cccggagtga tcaaaaggcg gccccccgga gcggggtcga ccttcaacgc
      481 cccggcccgc tcggcgacgg cgatccggat tcgttcctcg tcggaccggg gcgcctcgat
      541 cgcgaccgat cccggggttc cgtccgcgtc gtggccggcg gcgcggcgca cgtcatcgac
      601 gtaggaaagg acgtcggagg gatcgacctt gcgttgctcg ttgaaccgga ccaggcggcc
      661 cagatcggcc ctcatcgcca gcagaacggc cctggcaggt tcgccgtcgc gaccggcgcg
      721 accggcctcc tggtagtagg cctcgacgct cgaagggatc gcccagtgcc agactgacct
      781 gacgttggcc ttgtcgattc ccatgccgaa ggcattggtg gcgaccacga cctcggcgtc
      841 gccttccatg aaccggtgct gggccgatgc gcgctcgtcc ggagccatgc cggcgtggta
      901 tccgaccgcg gaaaggccgg ccgaccgcag gtcctcggcc agctcttcgg tgctcttgcg
      961 ggtgccgcaa tagacgatcg ccgggcggtt ggccgggtcg gccagaccca gctccagcag
     1021 cgcctgtttg cgggctttgg atcctttgcc ttcaagcggg atcgtgtcga agctcaggtt
     1081 tggccggtcg aagccggaat ggatttcggc cgggtcgttc atccccatcc gttcggcgat
     1141 ctcggccacc acctgcggag ttgccgtcgc ggtgcaggcc atcaccgcgg gccggccgag
     1201 ccgctcggcc accttgggca ggcgcaggta gtcgggccgg aagtcatggc cccattccga
     1261 caggcaatgg gcttcgtcga tcgccagcag gtcgatctcc cgggatccga tcagatcgag
     1321 aaaggcggcc gacccgaaac gctccgggga gcagtaggcg attcgcgccg aaccatcctt
     1381 gaccgcctgc cacgatgcgg ctgcgtcctc atcaccgaga ccggaggcga acatgaccgc
     1441 cgggtggcct tcgctgcgca gccgtttgac ctggtcggcc atcagcgcga tcagggggct
     1501 gaccacgatc gtgagcgacg gtgaggccag cccgggcaac tggtagcaga ggctcttgcc
     1561 gcctccggtc ggcatgacca cgaggctgtc gcggccttga agcgctgctt cgaccgcttc
     1621 gcgctggccg gggcggaaac tgtcgtaatc catgtgggcg agcaggccct cgggatcgcg
     1681 ggggccctcg taaagcc
//
LOCUS       DAOQKN010000321         1673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_64505_length_1673_cov_6.101360, whole genome shotgun sequence.
ACCESSION   DAOQKN010000321 DAOQKN010000000
VERSION     DAOQKN010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64505_length_1673_cov_6.101360"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="PLJ64_12940"
     CDS             <1..1131
                     /locus_tag="PLJ64_12940"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNC94437.1"
                     /translation="VKGISLFIVPRFLLDEDGSIGGRNDVVLAGVNHKMGFRGTVNTV
                     LGFGEGEYKPGGEAGAVGYLIGEPNRGLAYMFHMMNEARIGVGLGATALGYTGYLKSL
                     RYARERPQGRNLASKNPEDPQIPISEHTDVKRMLLAQKSYVEGALGLELYCSSLVDDE
                     MTAPDEDDRTRAGLLLEILTPIAKSWPSQWCLEANSLAIQVHGGYGYAREYDVEQHYR
                     DNRLNPIHEGTHGIQALDLLGRKVTMQGGAAFGALFEVITATIDEAKGAGGEAGELAD
                     QLAASLGRLAETTGKLWADRDPELALANATLYLEAAGHIVLAWIWLQQYLAAAGKEGE
                     FYDGKRQATRYFFRFELPKTGPQFDLLASLDRTTLEMEDAWF"
     gene            1134..>1673
                     /locus_tag="PLJ64_12945"
     CDS             1134..>1673
                     /locus_tag="PLJ64_12945"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HNC94438.1"
                     /translation="MPGESTDLPVVQMGAAQIAAAIRAGELTSAEAVEASIARIEEVN
                     PRLNAVVTPTFESAREAAAAADRQIAAQGSDGLPPLFGVPITIKDCYPVDGVRFTAGS
                     WFHRDEVADGDAVAVERLRKAGAIILGKTNIPDMCWGFESVNPVFGRTESARHRGYSA
                     GGSSGGEASIIAAGGSALGL"
BASE COUNT          304 a          546 c          566 g          257 t
ORIGIN      
        1 gtgaagggga tcagcctctt catcgttccg cggttcctgc tcgacgaaga cggttcgatc
       61 ggcggacgaa acgacgtggt cctggccggt gtcaaccaca agatgggctt ccgcgggacc
      121 gtcaacacgg tcctcggctt cggcgagggc gagtacaaac cgggcggcga agccggcgcg
      181 gtgggctacc tgatcggcga gccgaaccgg ggtctcgcct acatgttcca catgatgaac
      241 gaggcccgga tcggggtcgg tctcggcgcg acggcgctcg gttacaccgg gtacctcaag
      301 tcccttcgtt atgcgaggga acgcccccag ggccgcaacc tcgcttcgaa gaatccggaa
      361 gacccgcaaa tcccgatctc cgaacacacc gacgtgaagc gcatgctgct cgcacagaag
      421 agctacgtgg agggtgcgct cggcctcgag ctctactgct caagtctggt cgatgacgag
      481 atgaccgctc cggatgaaga cgaccgcacc agggccggtc tgctgctgga aatcctcacc
      541 ccgatcgcca agagctggcc ctcgcagtgg tgtctggaag ccaattctct ggcaatccag
      601 gttcacggtg gctacggcta cgcgcgcgag tacgacgtcg aacagcacta ccgcgacaac
      661 cggctgaatc cgatccacga aggaacccac gggatccagg cgctcgacct gctcggtcga
      721 aaggtgacga tgcagggcgg cgccgccttc ggcgcgctgt tcgaagtgat cacggcgacg
      781 atcgacgagg cgaagggggc cgggggcgag gcaggcgagc tcgccgatca gctggcggct
      841 tcactgggac ggctcgccga aacgacgggc aagctctggg ctgaccgcga ccctgaactc
      901 gccctggcca acgcgaccct gtacctcgag gcggccgggc acatcgtcct ggcctggatc
      961 tggctgcagc agtacctcgc tgcggccggc aaggaagggg agttctacga cggcaagcgg
     1021 caggcgaccc gctacttctt ccgcttcgag ctgccgaaga ccgggccgca gttcgacctg
     1081 ctggccagcc tcgaccggac caccctcgag atggaggacg cctggttcta gtcatgcccg
     1141 gggagagcac cgatctaccg gtagtccaga tgggcgcggc gcagatcgcc gcggccatcc
     1201 gggctggcga gctgacctcg gcggaggcgg tcgaggcgag catcgcccgg atcgaagaag
     1261 tcaatccgcg gctgaatgcg gtggtcaccc cgaccttcga atccgcccgg gaagccgccg
     1321 ccgcggccga ccggcagatc gccgcgcaag gctccgacgg gttgccaccg ctgttcggcg
     1381 tcccgatcac gatcaaggac tgctacccgg tcgacggggt gcgatttacc gcaggttcct
     1441 ggtttcaccg tgacgaagtg gccgatggtg acgcggtcgc cgtcgaacgc ctcaggaagg
     1501 ccggggcgat catcctcggc aagacgaaca tccccgacat gtgctggggc tttgaatcgg
     1561 tcaatcccgt ctttggccgc accgaaagcg ccaggcaccg tggctattcg gccgggggaa
     1621 gcagcggcgg cgaggcgtcg atcatcgccg ccggcggttc ggccctcggg ctc
//
LOCUS       DAOQKN010000322         1663 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_65282_length_1663_cov_3.843284, whole genome shotgun sequence.
ACCESSION   DAOQKN010000322 DAOQKN010000000
VERSION     DAOQKN010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65282_length_1663_cov_3.843284"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="PLJ64_12950"
     CDS             <1..505
                     /locus_tag="PLJ64_12950"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MsnO8 family LLM class oxidoreductase"
                     /protein_id="HNC94439.1"
                     /translation="PEIWLLGSSGYTAQVAGMLGMPYSFAHHFSAGNTEAAVQLYRSR
                     FEPSDFLEEPKVMIGVGVICAETDEEARWLAGPSRLAFARLRSGRPGRLPTPEEAEAH
                     DFTPDEEAVVSSVARGHIFGSPETVRTGLVDLIERTGADELILSTMVYEQKDRLRSYE
                     LVADIEL"
     gene            616..>1663
                     /locus_tag="PLJ64_12955"
     CDS             616..>1663
                     /locus_tag="PLJ64_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94440.1"
                     /translation="MQTRLPQTSSGRARGFSLAAALIVAAFVIGLAFWARPADALTVD
                     QTQSKIDAINSSVDDLKAQVAEDNRSIDALIGELSGLRARANELEAQLAAKQAELDAV
                     EAKLKQEREHLAEVRARLHRALKILSDQLVSLYVNGSPDVEEMVLASSDFGELVSSAG
                     YADAIQDRNESVITRVEDLRDQIKVMVEKMKVQQKKLEAARDEIAAETKQAEDARDAV
                     EAQKAEFEASSASRQAKIDALEAKAGSLEDSLPDLTMDPASSSAPRAVPPVAGSVAVI
                     NSDGTASPPADAPQAVKDVIAAGNQIIDLPYVWGGGHGSFESSGYDCSGTVSYALHGG
                     GLISSPLDSTGLTTW"
BASE COUNT          303 a          577 c          514 g          269 t
ORIGIN      
        1 gccggagatc tggctgctcg gctccagcgg ctacaccgcc caggtcgcgg ggatgctcgg
       61 catgccctat tccttcgccc atcacttcag tgccggcaac acggaggcgg cggtccagct
      121 ttaccgctcc cggttcgagc cctcggactt cctcgaagag ccaaaggtga tgatcggcgt
      181 cggggtgatc tgtgcggaaa ccgatgagga agcccgctgg ctggccggac cctcccgact
      241 cgccttcgcc cggctcagga gcggccggcc gggaagactc ccgactcccg aagaagcgga
      301 ggcgcacgat ttcaccccgg atgaggaggc ggtcgtctcc tccgtggcca ggggccatat
      361 attcggcagt cccgaaaccg tgcggaccgg cctggtcgat ctgatcgagc ggaccggagc
      421 cgacgaactg atcctctcga ccatggtcta cgaacagaag gaccgcctca ggtcctatga
      481 actggtcgca gacatcgagc tgtagagcca gtttccttca actgcacggt atttcccacg
      541 acccccggac acgacgactg tcgaccctga ggtcgctacc ttgctctttc cgcccgcgct
      601 ctgcggcggg cccttatgca gaccagactc ccgcaaacat ccagcggccg cgcccgcggc
      661 ttcagccttg ctgcggccct gatcgtggct gccttcgtga tcggtctcgc cttctgggcg
      721 cgaccggccg acgcgctgac cgtcgatcag acccagtcga agatcgacgc aatcaacagc
      781 tcggtcgatg acctcaaggc gcaggtagcc gaggacaacc gcagcatcga cgcgctgatc
      841 ggcgaacttt ccggtctgcg cgcccgcgcg aacgaactcg aggcccagct agccgccaag
      901 caggctgaac tcgacgcggt tgaagccaaa ctgaagcagg agcgggagca cctcgccgaa
      961 gttcgcgccc ggctgcaccg cgccctgaag atcctttcgg accagctcgt ctcgctctac
     1021 gtcaacggct ccccggacgt cgaggagatg gttctggctt cctcggactt cggtgaactg
     1081 gtctccagtg ccggatacgc cgatgcgatc caggatcgaa acgagtcggt gatcacccgg
     1141 gtcgaggacc ttcgcgacca gatcaaggtg atggtcgaga agatgaaggt ccagcagaag
     1201 aagcttgaag cggcccgcga cgagatcgca gccgaaacca agcaggccga ggacgcccgt
     1261 gacgcggtcg aggcccagaa ggccgaattc gaggcctcca gcgcctcccg ccaggcgaag
     1321 atcgacgcgc tcgaagccaa ggccggttcg ctcgaggact cgctgcccga cctgaccatg
     1381 gacccggcct caagttcggc tccccgggcg gtccccccgg tcgcgggatc ggtcgccgtg
     1441 atcaattccg acggcacggc atcgccaccc gctgacgctc cgcaggcggt caaggacgtg
     1501 attgccgcgg gcaaccagat catcgacctc ccctacgtgt ggggcggcgg tcacggatcc
     1561 ttcgaatcgt ccggttacga ctgttccggc acggtcagtt acgccctgca cggcggcggc
     1621 ctcatttcca gcccgctcga ctcgaccggc ctcaccacct ggg
//
LOCUS       DAOQKN010000323         1645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_66641_length_1645_cov_4.700000, whole genome shotgun sequence.
ACCESSION   DAOQKN010000323 DAOQKN010000000
VERSION     DAOQKN010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66641_length_1645_cov_4.700000"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..>1645)
                     /locus_tag="PLJ64_12960"
     CDS             complement(139..>1645)
                     /locus_tag="PLJ64_12960"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNC94441.1"
                     /translation="TPDQVQGIVDEVAEIQPAWADLRPSDRATYLKRAATVLHDRLDE
                     IASLLSREQGKPISECYTMELIPSIDALRWCAKEGPGILKDEKISYPQPFFKTKRSKF
                     RYQPIGVVGVIAPWNYPWTIPMQEIAIALMCGNGVVLKPASLTPLLGEAIQSVFEDAG
                     LPKGLIRNVHGGGRIGDALTKSTAGKIFFTGSVPVGYKVGEECAKLMKGCVLELGGKD
                     PMIVLEDANVDFAISGAVWGGFANAGQTCAGIERAYVRREVSEKFIEGVVAGASALKV
                     GDPSEVDTEIGPMVSKDQYELVCELVDDAVKSGATLRCGGPVEVEGFPDGNFIAPVVL
                     TDVTHEMRIMKEEIFGPVLPIITVESDQEALELANDSEFGLGSSVWTKDRQRGERIAD
                     RIEAGMVWINDHMFTHGACQCTWGGVKDSGLGHSHSKFGFYECVEIKLVTYEPGLTRN
                     FWWHPYDETLTQAMKSSASLLYGKGGQRIEALKSGAGPLLQVGKRITKRGS"
BASE COUNT          268 a          559 c          535 g          283 t
ORIGIN      
        1 cgctcgcgga ttcccgggca ggggctggct gaccggttgc acctgatttc gaagagtcgg
       61 attcctcccc gccgcgaccg aacagcagcc agccgccgcc gagcacgacg gcgaccagga
      121 cgatgaaggc gaagagcttc aggagcctcg tttggtgatc cgcttgccga cctgcagcag
      181 gggcccggca ccggacttga gtgcttcgat ccgctggccg cccttgccgt agagcaggct
      241 ggccgaggac ttcatcgcct gggtcagggt ctcgtcatag ggatgccacc agaagttccg
      301 ggtcaggccc ggctcgtagg tgaccagctt gatctccacg cactcgtaga agccgaactt
      361 cgaatgcgaa tggccgaggc cggaatcctt gactccgccc caggtgcact ggcaggcgcc
      421 gtgggtgaac atgtggtcgt tgatccagac catgccggcc tcgatccggt cggcgatccg
      481 ctcgccgcgc tggcggtcct tggtccagac cgacgagccc agcccgaact ccgaatcgtt
      541 ggccagttcg agcgcttcct gatcggactc gacggtgatg atcggcagca ccgggccgaa
      601 gatctcctcc ttcatgatcc gcatctcgtg cgtgacatcg gtcaggacca ccggggcgat
      661 gaagttgccg tccgggaaac cttcgacctc gaccggtccg ccgcagcgca gcgtggcgcc
      721 gctcttcacc gcgtcgtcga ccagctcgca gaccagctcg tactggtcct tgctgaccat
      781 cgggccgatc tcggtgtcca cctcggaggg gtcaccgacc ttcagcgccg aggccccggc
      841 gaccacgccc tcgatgaact tctcgctgac ctcgcgccgc acgtaggccc gctcgattcc
      901 ggcgcaggtc tgcccggcgt tggcgaaacc gccccagacc gcgccggaga tcgcgaagtc
      961 gacgttggca tcctcaagca cgatcatcgg atccttgcca ccgagctcga gcacgcagcc
     1021 cttcatcagc ttcgcgcatt cctcgccgac cttgtagccg accgggaccg agccggtgaa
     1081 gaagatcttg cccgcggtgg acttggtcag ggcgtcaccg atccggccgc cgccgtggac
     1141 gttgcggatc aggcccttgg gcagcccggc gtcctcgaag accgactgaa tcgcctcgcc
     1201 gagcagcggg gtcaggctgg ccggcttcag caccacgccg ttgccgcaca tcagggcgat
     1261 cgcgatctcc tgcatcggga tcgtccaggg gtagttccag ggggcgatca ccccgaccac
     1321 gccgatcggc tggtagcgga acttcgaacg cttggtcttg aagaagggct gcgggtagga
     1381 gatcttctcg tccttgagga tgcccgggcc ttccttcgcg caccagcgca gagcgtcgat
     1441 cgaggggatc agctccatcg tgtagcactc ggaaatcggc ttgccctgct cccgcgaaag
     1501 cagcgaggcg atctcgtcga ggcggtcatg cagaacggtc gcggcacgct tcaggtaggt
     1561 ggcgcggtcg gacggccgca ggtcggccca ggccggctgg atctcggcga cctcgtcgac
     1621 gatgccctgg acctggtccg gggtg
//
LOCUS       DAOQKN010000324         1638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_67221_length_1638_cov_5.013898, whole genome shotgun sequence.
ACCESSION   DAOQKN010000324 DAOQKN010000000
VERSION     DAOQKN010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67221_length_1638_cov_5.013898"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="PLJ64_12965"
     CDS             <1..624
                     /locus_tag="PLJ64_12965"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HNC94442.1"
                     /translation="IFSDIACPFCYIGTRQFAEALEAFPEDEVEVVWRSFQLDPEAPR
                     RAEGDLLDHLTRKFGISRDEARAMNDRVISMGHGAGVEFDFENARAVNTFDAHRMLKL
                     AAEQGLDGALADLFFEAYFTGSADLSDRADLARLASEAGVDPGRAAEVADGEEFATEV
                     RAEQELAGMLGITAVPTFVFDRSTALSGAQPVELMSRAIGQAISPAS"
     gene            679..1320
                     /locus_tag="PLJ64_12970"
     CDS             679..1320
                     /locus_tag="PLJ64_12970"
                     /inference="COORDINATES: protein motif:HMM:NF037470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QsdR family transcriptional regulator"
                     /protein_id="HNC94443.1"
                     /translation="MRNAAGSVTPRATKLQAALSDPAEPEDSGPLRALRLGISNFMAK
                     DRIDMSAMARQLDINRATLYRWVGSRDQFLVEMIWHLTATSLGRLDRECEATGPDRII
                     DVTIDFADEVIGNPGMKHWLATEGESAMSLLTRAGSGYQPRLIAWVEESIERQVDAGN
                     MALPEGVEPHELAYVIERVMGGYIYLDLITGDEPDARRAEPLLRMLLRSTELA"
     gene            complement(1364..>1638)
                     /locus_tag="PLJ64_12975"
     CDS             complement(1364..>1638)
                     /locus_tag="PLJ64_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC94444.1"
                     /translation="PMIPVTPEDAGAAEGQAAARYLMKDERPSLLFWADDDPALPLDP
                     VGRAVQMLFPMAEPLTVIENAGHFLQEDHGEEIGRQIATWLDRTAV"
BASE COUNT          264 a          552 c          564 g          258 t
ORIGIN      
        1 atcttctccg acatcgcctg ccccttctgt tacatcggca cccgccagtt cgcggaagcc
       61 ctggaggctt tccccgaaga cgaggtcgag gtcgtctggc ggagcttcca gctcgatccc
      121 gaagcgccgc gccgggcgga aggtgatctc ctcgaccatc tcacccgcaa gttcggaatc
      181 tcgcgggatg aagcccgggc gatgaacgac cgggtgatct cgatgggcca cggggccggc
      241 gtcgagttcg acttcgagaa cgcgagagcg gtcaatacct tcgacgcaca tcgcatgctg
      301 aagctggctg ccgagcaagg gctcgacggc gcgctcgccg acctcttctt cgaggcctat
      361 ttcaccggat cggccgacct ctccgaccgc gcggacctgg cccggctggc gagcgaggcc
      421 ggcgtagacc cgggccgggc agccgaagtc gctgacgggg aagagttcgc aaccgaggtg
      481 agggcggaac aggaactggc cgggatgctc gggatcaccg cggtgccgac cttcgtcttc
      541 gatcgttcaa cggcgctttc cggcgcccag ccagtcgaac tgatgagccg ggcgatcggg
      601 caggcaatct ccccggcgag ctgaaccgca gcgcagcctg gcccccgccg cgggcgcgat
      661 cgggcgcggt ttcccgaaat gcgaaatgct gccggttccg tgaccccgcg agccacaaag
      721 ctccaggctg cgctgagcga cccggccgag cccgaggatt ccggcccgct ccgggcgctc
      781 aggctcggca tcagcaactt catggccaag gaccggatcg acatgtcggc gatggcccgt
      841 cagctcgaca tcaaccgggc caccctttac cgctgggtcg ggtcgcggga tcagtttctg
      901 gtcgagatga tctggcacct gaccgcgacc agcctgggcc ggcttgaccg cgaatgcgag
      961 gcgaccgggc ccgaccggat cattgatgtc acgatcgatt tcgccgacga ggtgatcggc
     1021 aacccgggca tgaagcactg gctggcgacc gagggggagt cggcgatgtc gctgctgacc
     1081 agggccggtt cggggtatca gccgcgcctg atcgcctggg tcgaggaatc gatcgagcgt
     1141 caggttgacg ccggcaacat ggccctgccc gaaggggtcg agccccatga actcgcctac
     1201 gtgatcgagc gggtcatggg cggctacatc tacctcgacc tgatcaccgg cgacgagccc
     1261 gacgcccgcc gggccgagcc cctgctcagg atgctgctgc gctcaaccga actcgcctga
     1321 ccatctcttc cggccgcacc ggctggcggg gccggtctgc gggtcagacc gcggtgcggt
     1381 ccagccaggt ggcaatctgg cggccgatct cctcgccgtg gtcttcctgc aggaagtggc
     1441 cggcgttttc gatcacggtc agcggctcgg ccatggggaa gagcatctgg acggcgcggc
     1501 cgaccgggtc gagcggcagg gccggatcgt cgtcggccca gaacagaagc gaggggcgtt
     1561 cgtccttcat caggtacctg gccgcggcct gaccctcggc cgcgccggcg tcctcgggcg
     1621 tgaccgggat catcggcg
//
LOCUS       DAOQKN010000325         1634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_67526_length_1634_cov_5.604180, whole genome shotgun sequence.
ACCESSION   DAOQKN010000325 DAOQKN010000000
VERSION     DAOQKN010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67526_length_1634_cov_5.604180"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..957)
                     /locus_tag="PLJ64_12980"
     CDS             complement(<1..957)
                     /locus_tag="PLJ64_12980"
                     /inference="COORDINATES: protein motif:HMM:NF012588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase"
                     /protein_id="HNC94445.1"
                     /translation="MSLPRIPRDPENDYSREAAEARRDLVREQTGAELEHVGSYSFDP
                     AVLPGNIENFIGVAQMPVGLAGPLLIDGEHAQGEFYVPMATTEGTLVASYSRGMKMVA
                     EAGGVKTTIVDDRMQRAPAFIFDSAREAKEFNLWFSEHYDEIKAAAESTTSTGKLIEV
                     ERYHASRFLYTRFDYTTGDAAGQNMVSKATWAACEWIKAHYPPLMDYMLDAQLSTDKK
                     HSQINTLKTRGKRVIAEVQIPSAILEKHMRVTSRQVFQARQISNQGAMLSGSTANTPH
                     PANGIAAVFIATGQDEANVAESQAAIGYTELRGEDADYYYSIT"
     gene            complement(1010..>1634)
                     /locus_tag="PLJ64_12985"
     CDS             complement(1010..>1634)
                     /locus_tag="PLJ64_12985"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HNC94446.1"
                     /translation="QGTATAARTFTLTNTGSAPATLNTFVISGNEPVPFTISSDCDSI
                     PAGGSCTGQVEFATANPGAFQATAIYSFEDVAGDFRVNLTGGVTALTYKAKLKLAGPK
                     KFTAGKKVKVTATTANIGTAALDQVTLSWKAVQAGKTKARGQTSLATIAAGKSLARAI
                     NLAIPKAKLVRGKPVKLTVTASRDGKGTAGDFHVKQEFSLSVAVRQK"
BASE COUNT          230 a          500 c          553 g          351 t
ORIGIN      
        1 ggtgatcgag tagtagtagt cagcatcttc gccccgcagt tcggtgtagc cgatcgcagc
       61 ctgcgattcg gccacgttgg cttcgtcctg gccggttgcg atgaagaccg cggcgatccc
      121 gttggccggg tgcggggtgt tggccgtgct gccggagagc atcgcgccct ggttggagat
      181 ctgccgcgcc tgaaagacct ggcgggaggt gacccgcatg tgcttttcga ggatcgccga
      241 ggggatctgc acttcggcga tcacccgctt gccgcgggtc ttgagagtgt tgatctggga
      301 gtgcttcttg tcggtcgata gctgggcgtc gagcatgtaa tccatcagtg gcggatagtg
      361 cgccttgatc cattcgcagg cggcccaggt cgccttgctg accatgttct ggccggccgc
      421 atccccggtc gtgtagtcga accgggtgta gaggaagcga ctggcgtggt agcgctcgac
      481 ctcgatcagc ttgccggtcg aggtggttga ttccgcggcc gccttgattt cgtcgtagtg
      541 ctcgctgaac cagaggttga actccttcgc ctcccgggcc gagtcgaaga tgaaggcggg
      601 cgcccgctgc atgcgatcgt cgacgatcgt cgtcttgacc ccgccggctt cggcgaccat
      661 cttcatgccg cgactgtagg aagcgaccag ggttccctcg gtggtcgcca tcggcacgta
      721 gaactcgccc tgtgcatgtt cgccgtcgat caggagcggt ccggccaggc cgaccggcat
      781 ctgcgcgacc ccgatgaagt tctcgatgtt gccgggcagc accgcggggt caaaggagta
      841 ggagccgaca tgctcgagct cggccccggt ctgttccctg accagatccc gccgcgcctc
      901 cgcggcctca cggctgtagt cgttttcagg gtcgcgggga atcctgggca gactcatgcc
      961 gcgcaccata tccccgatcc gtcgccccgt tcaccccgac cggggccggc tatttctgac
     1021 ggacggctac cgacaagctg aattcctgct tgacgtggaa gtcgcctgcg gttcccttgc
     1081 cgtcgcggct ggcggtcacg gtgagcttga ccggtttgcc gcggaccagt ttcgctttgg
     1141 ggatcgcgag gttgattgcc ctggccaggg atttcccggc agcgatcgtc gccaggctgg
     1201 tctggccccg ggccttcgtc tttccggcct ggaccgcctt ccaggacagg gtcacctggt
     1261 caagcgcggc ggtgccgatg ttggcggtgg tcgcggtgac cttgaccttc ttgcctgccg
     1321 tgaatttctt cgggccagcc agtttcagtt tcgctttgta ggtgagagcg gtgacaccgc
     1381 cggtcaggtt cacgcggaag tcgccggcta cgtcttcgaa ggagtagata gccgttgcct
     1441 ggaatgcacc cgggttggcg gtggcgaatt cgacctgtcc ggtgcagctg ccgccagccg
     1501 ggatcgagtc gcaatcgctg ctgatcgtga agggcaccgg ctcgttcccg ctgatcacaa
     1561 aggtgttcaa ggtcgccggc gcagaaccgg tgttggtcag cgtgaaggtt ctggcggccg
     1621 tcgccgttcc ctgc
//
LOCUS       DAOQKN010000326         1610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_69457_length_1610_cov_3.513183, whole genome shotgun sequence.
ACCESSION   DAOQKN010000326 DAOQKN010000000
VERSION     DAOQKN010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69457_length_1610_cov_3.513183"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="PLJ64_12990"
     CDS             complement(<1..414)
                     /locus_tag="PLJ64_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94447.1"
                     /translation="MILSGENIPDYLDERPHLGDRVGTVTQVEMPDDGNINGVYIVQG
                     DRGSIVLKQGLPWVRIIETWELTAERTTREANLFEKWTPFAEGLLPEIFDFDADNHVL
                     AMEDLSGFAILADAFGADDQTANVERVGRLLARAFL"
     gene            complement(432..>1610)
                     /locus_tag="PLJ64_12995"
     CDS             complement(432..>1610)
                     /locus_tag="PLJ64_12995"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC94448.1"
                     /translation="HSWWNQIPTLSTICGREEAKLLADESVAAIERIGELSNRHPEVD
                     FRQSGWLWTASTEAQQGSWDACLAECDAQGVEPFAQLSGARLEEKTGTSVTRSGVFES
                     TGGTVNPQGLVDALKQEATELGVEVFERTPVLEVSGHGPASVRTPNGDTAAGKVILTT
                     NAWAARNRALQPYLYVVGSDIVLTNPLHEISDEHLPEVGLAACDSQPRVLYWSLRGDR
                     RLAFGRGGGRIAPFNRIGRHSGGATRWRRDVITSMKRVYPKLGSAEPATAWSGAVDRT
                     IDGFPIFGQTDRRGPLYGVGWSGSGVVGSALGGRILASLACERDDFWSGNRLVTRRPI
                     RLPREPLRTIGAHIVRSAVRQSGKAEEQGTIPNPVTRKVAAMAPSLEADPESGPGAKQ
                     "
BASE COUNT          262 a          540 c          497 g          311 t
ORIGIN      
        1 gagaaaggcc ctggcgagca ggcgacccac ccgttcgacg tttgcggtct ggtcgtcggc
       61 accgaaggca tcggccagga tggcgaaccc cgaaaggtcc tccatcgcca gcacgtggtt
      121 gtccgcgtcg aaatcgaaga tctccggcag gagtccctcg gcgaagggag tccacttttc
      181 gaacaggttc gcttcgcggg tggtccgttc ggcggtcagc tcccaagtct cgatgattct
      241 gacccagggc agtccctgct tcagaacgat cgaaccacgg tcaccctgca cgatgtagac
      301 gccgttgatg ttgccgtcat cgggcatctc gacctgggtc accgtgccga cacggtcacc
      361 gagatgcggt cgctcatcca ggtaatccgg aatgttttcc ccgctcagaa tcatgaagtc
      421 ggccggtcgg gtcactgttt cgctcccggc ccggactcgg gatctgcttc cagcgacggc
      481 gccatcgctg ccaccttcct ggtgaccggg tttggaatcg ttccctgctc ttcggctttg
      541 ccgctctggc gtacggcgga cctgacgata tgcgcgccga tcgttcgcag cggctcgcgc
      601 gggagccgga ttggccgcct cgtgacgagc cggttgccgc tccagaagtc gtcacgctcg
      661 caggcgagcg aagcgagaat cctgccgccg agggccgacc cgaccacacc gcttccgctc
      721 cagccgaccc cgtagagggg accccgcctg tcggtctggc caaagatcgg aaacccgtcg
      781 atggtccggt ccaccgcccc ggaccaggct gtggcgggct cggccgaccc gagtttcgga
      841 tagacgcgtt tcatcgaagt gattacgtcc cgacgccatc ttgtcgctcc gcccgagtgc
      901 cggccgatgc gattgaaggg agcgatcctg ccgccgccac ggccgaatgc gagccggcga
      961 tctccgcgaa gcgaccagta gagcactctc ggctgtgaat cacacgccgc cagtccgacc
     1021 tcgggaaggt gttcgtcgga aatctcgtga agcggattgg tcagcacgat gtccgagcca
     1081 accacgtaaa ggtacggctg aagtgctcgg ttccgggcgg cccacgcgtt ggtggtcagg
     1141 atgaccttgc ccgccgcggt atccccgttc ggggtcctga ccgacgccgg tccgtggccg
     1201 gaaacctcga gtaccggcgt cctttcgaag acctcgaccc cgagttcggt cgcttcctgc
     1261 ttcagcgcgt caacaagtcc ctgggggttt accgtcccac cggtagattc gaataccccg
     1321 gaccgggtga cgctcgttcc cgtcttttct tcaagtcttg cgccgctcag ttgcgcgaag
     1381 ggttcgacac cctgcgcgtc acactcggcg agacatgcgt cccagcttcc ctgctgtgct
     1441 tcggttgagg ccgtccagag ccagcctgac tgcctgaagt cgacctcggg gtgccggttc
     1501 gaaagctccc cgatccgctc aatggccgcc accgattcat cggcgaggag ctttgcttcc
     1561 tcgcgtccgc agatggtcga cagggtcggg atctgattcc accagctgtg
//
LOCUS       DAOQKN010000327         1609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_69513_length_1609_cov_5.835264, whole genome shotgun sequence.
ACCESSION   DAOQKN010000327 DAOQKN010000000
VERSION     DAOQKN010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69513_length_1609_cov_5.835264"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1205)
                     /locus_tag="PLJ64_13000"
     CDS             complement(<1..1205)
                     /locus_tag="PLJ64_13000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC94449.1"
                     /translation="MERKWLSLVAVITGIFMLLIDITIVNVALPDIGTDLDASFSSLE
                     WVVNAYTLTLASSLLVSGSLADLIGRKKVFAAGLIIFTLASLACALAETSLMLNLLRA
                     VQGIGGGMMFSTSLAIIAQTFQGRDRGIAFGIVGATTGAAVAIGPLLGGLLTDTFGWE
                     AIFFVNVPIGIFALIGTFRNVDESKDPHPSGIDWLGAVLFSAALFLMIYALVHGNDKG
                     WGSLEIIGAMVLSVVLLIIFGGVETHKEHPLFDLSLFRKPAFVGACLVAFCMSSSLFA
                     LFLFLTFYIQGGVLGFSPLEAGLRFLPITGMMLVAGPIAGRLTTKVQVRYLLSGGLLG
                     VALGIFLLHGIDVNSGWTALILGFVVAGVGSGFVNPPLASTAIGVVEQRRSGMASGIN
                     ATFRQAGIAA"
     gene            1309..1382
                     /locus_tag="PLJ64_13005"
     tRNA            1309..1382
                     /locus_tag="PLJ64_13005"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1343..1345,aa:Met,seq:cat)
BASE COUNT          319 a          490 c          556 g          244 t
ORIGIN      
        1 gcggcaatgc cggcctgccg gaaggtcgcg ttgatgccgg aagccattcc cgagcgtcgc
       61 tgctcgacga cgccgatcgc ggtcgaggcc aggggcgggt tgacgaagcc gctgccgaca
      121 ccggccacga cgaagccgag gatcagcgcg gtccagccgg agttcacatc gattccgtgc
      181 agcaggaaga tgccgagcgc gacgccgagc aggccgccac tcagcagata gcgaacctgg
      241 accttggtgg tcaggcggcc ggcgatcggg ccggccacca gcatcatgcc ggtgatcggc
      301 aggaaccgga gaccggcctc caggggagag aagccgagca cgccgccctg gatgtagaag
      361 gtgaggaaga ggaagagcgc gaacagcgat gacgacatgc agaaggcgac gaggcaggca
      421 ccgacgaagg ccggcttgcg aaagaggctc aggtcgaaga gcggatgttc cttgtgggtt
      481 tcgaccccgc cgaagatgat cagcaggacg accgagagca ccatcgcgcc gatgatttcc
      541 aggctgcccc agcccttgtc gttgccgtga acgagcgcgt agatcatcag gaagagcgcg
      601 gccgagaaga gcacggcgcc gagccagtcg attcccgacg gatgcgggtc cttggactcg
      661 tcgacgttgc ggaaggtgcc gatcagggcg aagatcccga tcggcacgtt gacgaagaag
      721 atcgcttccc agccgaaggt gtcggtcaga aggccgccga gcagcgggcc gatcgcgacc
      781 gccgccccgg tggtcgcccc gacgatcccg aaggcgatgc cccggtccct gccctggaaa
      841 gtctgggcga tgatcgccag ggaagtggag aacatcatcc cgccgccgat cccctggacc
      901 gcacggagca ggttcagcat cagcgaggtc tcggccaggg cgcaggccag ggaggcgagg
      961 gtgaagatga tcagcccggc cgcgaagacc ttcttgcggc cgatcaggtc agccagtgat
     1021 ccggagacca gcaggctgga ggcgagggtc agcgtgtacg cgttgaccac ccattcgagg
     1081 ctggagaacg aagcgtcgag gtcggtgccg atgtccggca gtgccacgtt gacgatcgtg
     1141 atgtcgatca gcagcatgaa gatgccggtg atgaccgcga cgagggagag ccacttcctt
     1201 tccaccttca ggaggctatc cagccggcct tcccggcccc gcaggagcct caccagcgcg
     1261 aacgggagca agccccaaag cccatgagtg ctagacttgg cggtccgccg cggggtggag
     1321 cagtcaggta gctcgtcggg ctcataaccc gaaggtcgca ggttcgaatc ctgcccccgc
     1381 tatgttcgcc agggtgaccg aatcaccttt cgcgaatagc tcctccgagt tgttcagcgc
     1441 cttcgcgact tcctctgcga tggtcgcgga aatttccacg cggccttccc gtttatcgaa
     1501 ggcgatttgg aggtcgaagg cctcgtagac ctgccgtctg atttgcggtg atgcgttccg
     1561 gaggctttcg ctgaggtccg ggatcgactc gaggcgctcg atcagccgg
//
LOCUS       DAOQKN010000328         1574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_72520_length_1574_cov_3.970375, whole genome shotgun sequence.
ACCESSION   DAOQKN010000328 DAOQKN010000000
VERSION     DAOQKN010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72520_length_1574_cov_3.970375"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(164..367)
                     /locus_tag="PLJ64_13010"
     CDS             complement(164..367)
                     /locus_tag="PLJ64_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94450.1"
                     /translation="MRYELLEPSGICLEAFDCRDHAVEILIGMVADDSSNREITILEI
                     DDENEETLGFIEVDEELLASSTG"
     gene            457..1221
                     /locus_tag="PLJ64_13015"
     CDS             457..1221
                     /locus_tag="PLJ64_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC94451.1"
                     /translation="MNPLNDLAKTVGFRRAGRTFDSPPPRFDPDGPPSTRELANEMLA
                     VAQLPRLFASSPRLLAAPRGDGSVVFDVPGWKAPEVSMQPIRTYLSCLGYDARTWGHG
                     TNRGSVEADVLQFRTTVERAAEISERPVGLVGWSLGGVIAREVAREIPEAVSGVVTFG
                     SPIVGGPAHTVAARNYTEKESRRFEELIEKVNRENPLGVPITAIFTRRDGVVNWRACI
                     DRNTEGVRHVEVGSSHLGLGLDPDVWLATAEALAAA"
     gene            1221..>1574
                     /locus_tag="PLJ64_13020"
     CDS             1221..>1574
                     /locus_tag="PLJ64_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94452.1"
                     /translation="MAGAQLKLVTWNVAGRTRLLTQQAAALSRTEPDLVTLQEIRKGT
                     VERWKAALAEDGLVHTIDSGYRTAERRLFNLTASRWKLSDLGDAPVPQPERVVSAALD
                     TPFGPCEIHNVHVPPA"
BASE COUNT          296 a          518 c          481 g          279 t
ORIGIN      
        1 cttccggtct ttcacgtccg gccaacgaac ctgtgcctca tcgagcagtt tctcgaggtc
       61 ccccgtgtca gtcaccgtgt atcggggctt cgttgttggc atcgccagag tgtagcacca
      121 aaccatttgg gtgtcgcacc ctcaaagtct ggctcttgaa gccttagccc gttgacgacg
      181 cgaggagttc ttcgtcgact tcaatgaagc cgagcgtttc ttcgttttcg tcgtcgatct
      241 cgagaatcgt gatttcgcgg ttgctggaat cgtcggcgac catgccgatg aggatctcga
      301 ccgcgtgatc gcgacaatcg aatgcttcaa ggcagattcc ggatggctcc aggagctcgt
      361 aacgcatgta agcgaaccta ggtaacgggt cggacggaaa cgggcgctgg aaggaagtcg
      421 ggtttcagac ggccccaaac tggaaaccat ccccccgtga atcccctcaa cgacctggca
      481 aagaccgttg gcttccggcg cgccggcagg accttcgact cccctccgcc ccgattcgac
      541 ccggacggac ccccttcgac ccgggaactc gcaaacgaga tgctcgcggt tgcccagctg
      601 ccgcgcctgt tcgcgtcctc accgcgactc ctcgccgccc ccagagggga cggatcggtt
      661 gtcttcgacg tacctggctg gaaggcccct gaggtctcga tgcagccgat ccggacgtat
      721 ctctcctgcc tcggctacga cgccagaacc tggggacacg gaaccaaccg cggcagcgtc
      781 gaggctgacg tgctccagtt ccggacgacg gtggaaaggg cggcagagat ctcggaacgg
      841 ccggttggac tggtcggatg gagcctcggt ggagtaatcg cccgggaagt cgcgagagag
      901 attccggagg cggtttcggg cgtcgtcacc ttcggcagcc cgatcgtggg cggaccagcc
      961 cataccgtcg ccgcacgcaa ctacaccgag aaggaaagcc gccgcttcga ggaactgatc
     1021 gagaaggtaa atcgcgagaa tccgctggga gtgccgatta ccgcaatctt cacccggcgc
     1081 gacggggtcg tgaactggcg cgcttgcatc gaccgaaaca ccgagggtgt ccgtcacgtc
     1141 gaagtcggct cgagccatct cggtctggga ctggatcccg acgtctggct ggccaccgcc
     1201 gaggccctcg ctgcggcctg atggccggtg cacagctcaa actggtcacc tggaacgtgg
     1261 ccggtcgcac tcgccttcta acgcaacagg ccgccgcact cagccggacc gaacctgacc
     1321 tggtcacgct gcaggagata cgaaaaggca ccgtcgagcg atggaaggcg gccctggccg
     1381 aagacggact tgtccacacc atcgactccg gctaccggac cgcggaacgg cgactattca
     1441 acctgaccgc ctcgcggtgg aagttgagcg acctcggaga tgcgccagtc ccccagcccg
     1501 agcgcgtcgt ctcggccgcc ctggacacgc ccttcgggcc ctgcgagatt cacaacgttc
     1561 acgtccctcc cgct
//
LOCUS       DAOQKN010000329         1569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_72938_length_1569_cov_4.379789, whole genome shotgun sequence.
ACCESSION   DAOQKN010000329 DAOQKN010000000
VERSION     DAOQKN010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72938_length_1569_cov_4.379789"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1294)
                     /locus_tag="PLJ64_13025"
     CDS             complement(<1..1294)
                     /locus_tag="PLJ64_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNC94453.1"
                     /translation="MSETREVDLVIKGGTVVSPSGTRKVGIAVDGGKVVAVTADEFLP
                     PAKKTVDASGLHVIPGLVDTEAHPGCYSPLKDDLASESIAAVTAGVTTWGVHAPSTRM
                     GHPDFAEYVQKEDVMSFHDSFPYFEALTESDSAIDIFLTYMLETDQQAEEIPEYAKDH
                     GVTSYKLYLQAMSPESEPNWPGRRAGLGAGFDDGVCWVTMENVAALGRPGVVCMHCEN
                     WEIARIFDKRLKEQGRTDWATWSDRSPHYLEASHIRWYGDMAAEIGCPIYIQHSTTPE
                     SYKEILELRGRGVEIYAQTGPHWLHFGKGEHNAWRINVPLRSRENNPNIWTALQCGII
                     DSVGSDHVVAWEPADYESSYNENIWELKTGFTSRVEMHLPVLLEGVHQGKLTIERLVE
                     VACANPARIFGVYPQKGLIEVGADADIVLVDLNKEVTVK"
BASE COUNT          253 a          491 c          517 g          308 t
ORIGIN      
        1 tcttgaccgt gacttccttg ttcaggtcga ccagcacgat gtcggcgtcg gcgccaacct
       61 cgatcaggcc cttctgtggg tagaccccga agatccgggc cgggttggcg caggccacct
      121 cgaccagacg ctcgatcgtg agcttgccct gatggacccc ttcgagcagg accggcaggt
      181 gcatctcgac ccttgaggtg aagccggtct tgagctccca gatgttctcg ttgtaggagc
      241 tttcgtagtc ggcgggctcc caggcgacga cgtggtcgga gccgaccgag tcgatgatcc
      301 cgcactgcag cgcggtccag atgttcgggt tgttctcccg cgaacggagc ggcacgttga
      361 tccgccaggc gttgtgctcg cccttgccga agtggagcca gtgcgggccg gtctgggcgt
      421 agatctcgac cccgcggccc cgcagctcga ggatctcctt gtaggactcg ggagtggtcg
      481 agtgctggat gtagatcggg cagcctatct cggcggccat gtctccgtac cagcggatgt
      541 gcgaggcctc gaggtagtgg ggcgagcggt ccgaccaggt tgcccagtcg gtccggccct
      601 gctccttgag ccgtttgtcg aagatccggg cgatctccca gttctcgcag tgcatgcaga
      661 ccacgccggg acggccgagg gcggccacgt tctccatggt cacccagcag acaccgtcgt
      721 cgaaaccggc gccgagcccg gcccggcggc ccggccagtt cggttccgac tccgggctca
      781 tcgcctgcag gtagagcttg tatgaggtga cgccgtggtc cttcgcgtat tcggggatct
      841 cctcggcctg ctggtcggtt tccagcatgt aggtgaggaa gatgtcaatc gccgaatcgg
      901 attcggtcag cgcctcgaag taggggaagg aatcgtggaa ggacatcacg tcttccttct
      961 ggacgtactc ggcgaagtcg gggtgaccca tccgggtcga cggggcatgg actccccagg
     1021 tggtaacgcc cgcggtcacc gcggcgattg attcgctggc aaggtcatcc ttgagcggcg
     1081 agtagcagcc cgggtgggcc tcggtatcaa ccaggcccgg gatcacgtga agtccgctcg
     1141 cgtcaacggt cttcttcgcg ggcggcagga actcgtcggc cgtgactgcc acgaccttgc
     1201 cgccgtcgac cgcaatgccc accttgcggg tgccgctggg actgaccacg gttccgccct
     1261 tgatgaccag atcaacttcg cgggtttcgc tcactttgct ctcctgtcag agctcccgga
     1321 aaccgggaga ctgtccagcc gtttgttcaa catgctgaac caagttctat atcgtggacg
     1381 aaaggtaaca cagaacgcgg cggccgggca atggtttctc ctgtacggcc ggacgaaata
     1441 cgcccggccg cctcgtcccg ttgatcagcg gtccgaggaa acaccggcgg tggccatgat
     1501 ttcctgcgag gcctgccgca gcacttcacc gatttcggtg agccggtcgc cgtcgagctg
     1561 ggtcgggaa
//
LOCUS       DAOQKN010000330         1558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_73918_length_1558_cov_3.461743, whole genome shotgun sequence.
ACCESSION   DAOQKN010000330 DAOQKN010000000
VERSION     DAOQKN010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73918_length_1558_cov_3.461743"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="PLJ64_13030"
     CDS             <1..373
                     /locus_tag="PLJ64_13030"
                     /inference="COORDINATES: protein motif:HMM:NF024639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNC94454.1"
                     /translation="QMMDGAELIALQHNRYWKREDGLVLDVGAFSAALEYAAETEAVV
                     VGKPSPDFFRMALADVDASADRAVMVGDDIEGDIGGGLASGITSVLVRTGKYKPEKVA
                     ESGIEPTATIDSIADLPQLLS"
     gene            384..1352
                     /locus_tag="PLJ64_13035"
     CDS             384..1352
                     /locus_tag="PLJ64_13035"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNC94455.1"
                     /translation="MLAIRIDDFGDPSVLKLQEVADPVAGPDQVLIEVDTSGVNFGDV
                     LVRRGEYFGRTALPVIPGWEVVGKAEAADPDGAFEAGDRVVALLDGEGYAERVAAPLR
                     DVVPVPDDVSDQVALAMVIQGATAWRLLEEMKPGQSVLISGAGSGVTHLTVQLARHLE
                     AAEVVVISSGGEKTERVRELGADLVVESTGDDTLQAQLAEELDGRKFDLAIDMVGGET
                     LQAMLRQLRPGGRAVIYGVAGGVPAKVNTGALLKNGLTVSGLWLGHSEGWSPREIVAR
                     LFDLVQSGKLSPTLGPTFPLAEAAGAHLAVEARTGVGKVALDCRNP"
     gene            complement(1359..>1558)
                     /locus_tag="PLJ64_13040"
     CDS             complement(1359..>1558)
                     /locus_tag="PLJ64_13040"
                     /inference="COORDINATES: protein motif:HMM:NF024591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94456.1"
                     /translation="EITAFIVPADDETRLEVDDLRDHLKQGLASYKVPKRWFLVEEIP
                     RTRAGKTDRSPERLFEAATEF"
BASE COUNT          256 a          484 c          545 g          273 t
ORIGIN      
        1 ccagatgatg gacggggccg agctgatcgc cctccagcac aaccgctact ggaagcgcga
       61 agacggactg gtgctagacg tcggcgcctt ctctgccgcc ctcgaatacg ccgcggagac
      121 cgaggcggtc gtggtcggca aaccctcgcc ggatttcttc cggatggctc tggcggacgt
      181 tgacgccagc gctgaccggg cggtgatggt cggtgacgac atcgaaggcg acatcggcgg
      241 cggacttgcc tccggcatca cctcggtcct ggtccgcacc ggcaagtaca agccggagaa
      301 ggtcgctgag tccggaatcg aacccaccgc cacgatcgac tcgatcgccg atctgccgca
      361 gctcttgtcg tagcctcccg accgtgctgg cgattcgcat agacgacttc ggggatccct
      421 cggtcctgaa gcttcaggaa gtggcggacc cggtggccgg gccagatcag gttttgatcg
      481 aggtcgacac ttcgggggtg aacttcgggg acgtgcttgt ccggcggggc gagtacttcg
      541 gccgcacggc gctgccggtg attcccgggt gggaggtcgt gggcaaggcc gaggcggccg
      601 acccggatgg cgcctttgaa gcaggagacc gggtggtcgc cctgcttgac ggcgagggtt
      661 acgccgaacg ggttgccgct cccctgcggg acgttgtccc ggtgcctgac gatgtctccg
      721 atcaggtggc gctggcgatg gtgattcagg gcgcgaccgc ctggcgcctg ctcgaggaaa
      781 tgaagccggg gcagagcgtc ctgatcagcg gggccggcag tggcgtcacc catctgacgg
      841 tccagctcgc tcgccacctc gaagcagcgg aggtcgtcgt catctccagc gggggagaga
      901 aaaccgagcg ggtgcgcgag cttggcgccg acctcgtggt tgagtcaacg ggcgacgaca
      961 cgcttcaggc gcagctggcc gaagaacttg acggccggaa gttcgatctc gcgatcgaca
     1021 tggtcggtgg ggagactctc caggcgatgc tccggcagct ccggcccgga ggtcgcgcgg
     1081 tgatttacgg ggtggctggg ggagtaccgg ccaaggtaaa taccggcgcg ctactcaaga
     1141 acggcctgac cgtctccgga ctctggctcg gacattcgga gggatggtca ccccgcgaga
     1201 tcgtcgcccg gcttttcgac ctggttcagt ccggaaagtt gagcccgact ctcggtccga
     1261 ctttcccgct ggccgaggcc gccggagccc acctcgcggt cgaggcccgg accggagtcg
     1321 gcaaggtggc tctggactgc cggaatccct gacggtgctc agaactccgt agctgcttca
     1381 aaaagccgct ccggcgagcg gtcggttttg cccgcccgag tccgcggaat ctcctcgacg
     1441 agaaaccacc gcttggggac cttgtaggag gcgagcccct gcttcaggtg gtccctcagg
     1501 tcatcgactt cgagtcttgt ctcgtcgtca gcggggacga taaaagcagt gatctcct
//
LOCUS       DAOQKN010000331         1539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_75638_length_1539_cov_5.657682, whole genome shotgun sequence.
ACCESSION   DAOQKN010000331 DAOQKN010000000
VERSION     DAOQKN010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75638_length_1539_cov_5.657682"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="PLJ64_13045"
     CDS             complement(<1..734)
                     /locus_tag="PLJ64_13045"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HNC94457.1"
                     /translation="MRLIITEKNNSAQKIAEILSHGAATETKSFTVPVFRWEDSDGET
                     AVIGTGGHFVGREFPQEKEYKQWKLDLIPGLIDAPLETGPIDGKKNVIKAVQKEAKKA
                     DSLVIGTDFDREGELIGLEALEVCLEVNPDLEHTLERARYSALTREEIEGAFANLDQL
                     SYPLANAAGARQDIDLIWGAAFTRAVSLVAKAYGANFLSVGRVQSPTLGLIVERELER
                     RAHVAKPFWELFARFEHPSGHSFEAH"
     gene            831..>1539
                     /locus_tag="PLJ64_13050"
     CDS             831..>1539
                     /locus_tag="PLJ64_13050"
                     /inference="COORDINATES: protein motif:HMM:NF013255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNC94458.1"
                     /translation="MKTLVTGGAGFIGSHLADALLARGDEVTVLDNFSTGKHQNLESA
                     LAAGADLIEGDILDRELVEKTLQSVRPQTVFHLAAQGEVQRSIEEPAFDATVNVVGTV
                     NLIEASKEIGLDRFVFASTGGAIYGEGSRIDLPASESGETAPLCQYGLSKRAGEMYLE
                     LYRRMYMFNSVALRFANVYGPRQCPKGEAGAVAIFGELLMAGRNPTVFGDGTQTRDFV
                     YIDDLIDAILTASRTDVE"
BASE COUNT          263 a          507 c          480 g          289 t
ORIGIN      
        1 tgatgggcct cgaaactgtg gccgctcgga tgctcgaacc tggcgaagag ctcccagaac
       61 ggcttggcga cgtgcgcccg ccgctccagc tcccgctcga cgatcaggcc gagggtcggg
      121 ctctgcaccc ggccgaccga aaggaagttg gccccgtagg ccttggcgac cagcgagacg
      181 gcgcgggtga aggcggcacc ccagatcagg tcgatgtcct ggcgggcgcc ggccgcgttg
      241 gcaagcgggt aggagagctg gtcgaggttg gcgaaggcgc cctcgatctc ttcccgggtc
      301 agggccgagt aacgcgccct ttcaagtgtg tgctcgagat cggggttcac ctcgaggcag
      361 acttcgagcg cctcgaggcc gatcagctcg ccctcgcggt cgaagtcggt gccgatcacc
      421 aggctgtcgg ccttcttggc ttccttctgg acggccttga tcacgttctt cttgccgtcg
      481 atcgggcccg tctccagcgg cgcgtcgatc aggcccggga tcaggtcgag cttccactgc
      541 ttgtattcct tctcctgcgg gaactcccgc ccgacgaagt gcccaccggt accgatcacg
      601 gcggtttctc cgtccgaatc ttcccagcgg aacacgggca cggtgaacga cttggtttcg
      661 gtcgcagcgc cgtgcgagag gatctccgcg atcttctggg ctgagttgtt tttctcggtg
      721 atgatgagtc gcattggctg gcgagagtag caggggcgcc gaaagacatt cggggaaaac
      781 ccggccaaag cccagtttgt tcacagtttt gttgcctaag attcacaccc atgaagaccc
      841 ttgtcacagg cggcgccgga ttcatcggat cgcatctcgc cgatgctctc ctcgcacgcg
      901 gtgacgaagt caccgtgctc gacaatttct ccaccggcaa gcaccaaaac ctcgaatcgg
      961 cgctggccgc gggagccgat ctgatcgagg gcgacatcct cgaccgggaa ctcgtggaaa
     1021 aaactttgca gtcggtccgc ccgcagaccg ttttccatct tgcggcccag ggcgaggtcc
     1081 agcgttcgat cgaggaaccg gccttcgacg cgaccgtgaa cgtggtcggc acggtcaacc
     1141 tgatcgaagc ttcgaaggag attggtctcg accgcttcgt cttcgcctcg accggcgggg
     1201 cgatctacgg cgaaggaagc cggatcgatt tgcccgccag cgaatccggg gaaaccgccc
     1261 cgctctgcca gtacgggctt tccaagcggg ccggcgagat gtacctcgag ctctaccggc
     1321 gcatgtacat gttcaactcg gtcgccctcc gcttcgccaa tgtctacggc cctcgccagt
     1381 gtcccaaggg tgaagccggc gcggtcgcga tcttcggcga gctcctgatg gccggccgga
     1441 acccgaccgt cttcggcgac ggcacccaga cccgcgactt cgtctacatc gacgacctga
     1501 tcgacgccat cctcaccgcc tcccgcaccg acgtcgaag
//
LOCUS       DAOQKN010000332         1515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_78057_length_1515_cov_3.639726, whole genome shotgun sequence.
ACCESSION   DAOQKN010000332 DAOQKN010000000
VERSION     DAOQKN010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78057_length_1515_cov_3.639726"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="PLJ64_13055"
     CDS             <1..813
                     /locus_tag="PLJ64_13055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94459.1"
                     /translation="DPEAADPAPVPGGNGNAIVPENSTLEKAIVTGPDVRAWYRGISQ
                     GGIMGGALMALAPDFDKGSLGVGAMNYSVLLTRSGSWNTYGSIFNPSYPNEIERPLAL
                     SLIQMLWDRGEPNGYAHRMTTDPLPDTPPHKILFDLAFGDHLVTNWQSNVEARTIGAK
                     AVSPFVADGRWAGVDGQWGIDPVASFPYDGSAIAYWDSGPLRPGLAPDSTIGTDPPPL
                     TNTAPAAGEDPHELPRVSDTAVEMIDSFLREGGAVTNPCAPGPCLAGGWTGS"
     gene            958..>1515
                     /locus_tag="PLJ64_13060"
     CDS             958..>1515
                     /locus_tag="PLJ64_13060"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNC94460.1"
                     /translation="MLRVVTLGALVVAIVFVASLIFKGDSGYKYSFDFQSASGMVSGN
                     FVMIGGSPVGKVTKVELTEDNRVKMEVEIDRELHEGTSAVIRKSSLSSLHNHYISLQP
                     GPDNEPVLPEGHDFGEGVTQTAVELDQFFDTFDETTRKGWSNWIQGIAAIYAGEGAEG
                     ANKTFKYSGAAFSSTQRLMAELADQD"
BASE COUNT          293 a          488 c          475 g          259 t
ORIGIN      
        1 gacccggaag ccgcagaccc ggcgccggtc ccgggtggca acggcaacgc aatcgtcccg
       61 gagaacagca ccctggagaa ggcgatcgtg accggcccgg atgtccgggc ctggtaccgc
      121 ggcatcagcc agggcgggat catgggtggt gccctgatgg cgctggcccc ggacttcgac
      181 aagggctcgc tcggggtggg ggcgatgaac tactcggtcc tgctgaccag gtccggctcc
      241 tggaacacct acggctcgat cttcaacccg tcctatccga acgagatcga gcggccgctg
      301 gccctgagtc tgatccagat gctttgggac agaggcgagc caaacgggta tgcccatcgc
      361 atgaccacgg atccgctgcc tgacactccg ccacacaaga tcctctttga cctcgccttc
      421 ggcgatcacc tcgtgaccaa ctggcaatcg aacgtcgaag cgcgcacgat cggggcgaag
      481 gcggtctcgc cgttcgtcgc cgacggccgc tgggccgggg tggacggtca gtgggggatc
      541 gatccggtcg ccagctttcc ctatgacggc tcggcgatcg cctactggga cagcgggccg
      601 ctccgacctg gtctggcccc ggattcaacg atcggaaccg atccgccacc gctcaccaat
      661 acggccccgg ccgcgggcga agatccgcat gaattacccc gggtttccga cactgcagtc
      721 gagatgatcg acagcttcct ccgggagggc ggagccgtca ccaatccctg tgctccgggt
      781 ccctgcctcg ctggcgggtg gaccggctcc tgacgtcagg ctctaacgcc gggcgctcag
      841 gtagcatgcc cccatcggaa ggaggcttct taggtgggga gaatcaccgg gtagctcaga
      901 ttgacgactg ccaatcaagc aaatccaagt cgcgtcaatc gcggacgtag ctcgaccgtg
      961 ctcagagttg tgactctggg cgcgcttgta gttgcgatcg ttttcgtcgc ttccctcatt
     1021 ttcaagggag attcaggcta caagtacagc ttcgatttcc agagcgcgag cggcatggtc
     1081 tcgggcaact tcgtgatgat cggcggctcg cccgtgggca aggtcaccaa ggtcgagttg
     1141 accgaggaca accgggtgaa gatggaggtc gagatcgacc gcgagcttca cgaaggcacc
     1201 tcggcggtca tccgcaagag ttccctttcc tcgctgcata accactacat ctcgctgcag
     1261 cccggcccgg acaatgaacc agtactcccc gaaggccatg atttcggcga aggcgtaacc
     1321 cagacggcgg tcgagctcga ccagttcttc gacacctttg acgagaccac ccgcaaaggc
     1381 tggtcgaact ggatccaggg catagccgcg atctacgccg gcgaaggggc cgaaggcgca
     1441 aacaagacct tcaagtacag cggggccgcc ttctcaagca cccagcggct gatggctgaa
     1501 ctggccgacc aggac
//
LOCUS       DAOQKN010000333         1513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_78246_length_1513_cov_3.288752, whole genome shotgun sequence.
ACCESSION   DAOQKN010000333 DAOQKN010000000
VERSION     DAOQKN010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78246_length_1513_cov_3.288752"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="PLJ64_13065"
     CDS             complement(<1..390)
                     /locus_tag="PLJ64_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC94461.1"
                     /translation="MSEGAGETEALPAVVEAGAYWPRPWSGEDGGPRRLCKPEGQPGL
                     GIQPGEDLQLEVFRDSFAADMLIRREPGELYALRHDMPRGDPLAGDCEGWVEKLDPET
                     LKPLTVTQRFPAGRYWPGGIAAHANGDI"
     gene            complement(387..794)
                     /locus_tag="PLJ64_13070"
     CDS             complement(387..794)
                     /locus_tag="PLJ64_13070"
                     /inference="COORDINATES: protein motif:HMM:NF018717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HNC94462.1"
                     /translation="MSPVIDPTPEQFKALVESDDDSPIVMVNLIRFKDQATGIDEGRT
                     GAEAYATYGQNIARYLAEVGGEVLIATASVESIIGPEQAEWDAVLLVRYPSRKAFIQM
                     ITNPGYQKEHEHRAAGVEEARLILSGLQYLKES"
     gene            complement(791..>1513)
                     /locus_tag="PLJ64_13075"
     CDS             complement(791..>1513)
                     /locus_tag="PLJ64_13075"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNC94463.1"
                     /translation="PDFNEADVSDKASNVKNLPLLDEAKIETVTASYRGRLGAVKSID
                     DQVGKIVKTLKKNRELNNTVIIFNSDNGYLQGEHRLAASKFLPFENSLRIPLIMRGPG
                     IKAHATVSNPSLDVDIAPTILAAAKAKAKPGRLFDGISLLAAAKKVKKLPYRDIPMEA
                     ERPLFKFTTPLTQFDLPFYGVKTNRYKYVHWSFGDVELYDMKKDPYELENLASDPDMA
                     GVVSQLEAKAQQLQDCKGKTCR"
BASE COUNT          212 a          486 c          509 g          306 t
ORIGIN      
        1 gatgtccccg ttcgcgtggg cggcgatacc gcccggccag tagcggccgg cggggaagcg
       61 ctgggtgacg gtcagcggct tgagggtttc ggggtcgagc ttttcgaccc agccctcaca
      121 gtcaccggcc agagggtcgc ctctcggcat gtcgtggcgg agcgcgtaga gctcgcccgg
      181 ctcgcgccgg atcagcatgt cggcggcgaa gctgtcgcgg aagacttcga gctgaaggtc
      241 ctcaccgggc tggatgccga gcccgggctg gccttccggt ttgcagagtc gccgcgggcc
      301 tccgtcctcg ccggaccagg gcctcggcca gtaggcgccg gcctcgacca cggccggcag
      361 cgcttcggtc tccccggccc cttcgctcaa gactctttga ggtactgcag gccggaaagg
      421 atcaggcggg cttcctcgac cccggctgcc cggtgctcat gttccttctg gtagcccggg
      481 ttggtgatca tctggatgaa tgccttccgc gacgggtagc ggaccagcag caccgcgtcc
      541 cactcggcct gctccggccc gatgatgctt tcgacgctgg cggtggcgat cagcacctcg
      601 ccgccgactt cggccaggta gcgggcgatg ttctggccgt aggtggcgta tgcctccgcc
      661 ccggtccggc cctcgtcgat tccggtcgcc tgatccttga agcggatcag gttgaccatc
      721 acgatcggcg aatcgtcatc ggactcgacc agggccttga actgctccgg agtcggatcg
      781 atcacgggac tcaccggcag gtcttgccct tgcagtcctg gagctgctgc gccttggcct
      841 ccagctggct gaccacgccc gccatgtccg ggtcgctggc caggttctcg agctcgtacg
      901 gatccttctt catgtcgtag agctcgacgt caccgaagga ccagtggacg tacttgtacc
      961 ggttggtctt gacgccgtag aagggaaggt cgaactgggt cagcggggtc gtgaacttga
     1021 acagcggccg ctccgcttcc atcgggatgt cgcggtaggg cagcttcttg actttcttcg
     1081 ccgcggccag cagggagatg ccgtcaaaga ggcgacccgg cttcgccttc gctttcgcgg
     1141 cggcgaggat cgtcggggcg atgtccacgt caaggctcgg gttggagacc gtggcgtggg
     1201 ccttgatccc ggggccccgc atgatcaggg ggatccgcag cgaattctcg aagggcagga
     1261 acttggatgc ggccaggcgg tgctcaccct gcaggtagcc gttgtccgag ttgaagatga
     1321 tcaccgtgtt gttcagctcg cggttcttct tcagcgtctt gacgatcttg ccgacctggt
     1381 cgtcgatcga cttcaccgct cccagccggc cgcgatagct ggcggtgacc gtttcgatct
     1441 tcgcctcgtc cagcagcggc aggttcttca cgttgctcgc cttgtcggaa acgtccgcct
     1501 cgttgaagtc ggg
//
LOCUS       DAOQKN010000334         1512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Solirubrobacterales bacterium isolate AMR_MDS_0573
            NODE_78318_length_1512_cov_4.555251, whole genome shotgun sequence.
ACCESSION   DAOQKN010000334 DAOQKN010000000
VERSION     DAOQKN010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810205
            Sequence Read Archive: SRR3501857
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Solirubrobacterales bacterium (wastewater metagenome)
  ORGANISM  Solirubrobacterales bacterium
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia;
            Solirubrobacterales.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:27:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,617
            CDSs (total)                      :: 2,568
            Genes (coding)                    :: 2,561
            CDSs (with protein)               :: 2,561
            Genes (RNA)                       :: 49
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Solirubrobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78318_length_1512_cov_4.555251"
                     /isolate="AMR_MDS_0573"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1909295"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-10"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="PLJ64_13080"
     CDS             complement(<1..804)
                     /locus_tag="PLJ64_13080"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HNC94464.1"
                     /translation="MSALSGVIVADFSRVLAGPLAAMTLGDLGANVIKVEPPGGDETR
                     GWVPPVDAAGRSTYFQTANRNKRSITLDLKQEEDRALAFELAMRADVLIENFRPGTMA
                     RFGLDWETVHAAHPSLVYCSITGFGSGPGAAMPGYDPLIQALSGMMSITGPPDGEASK
                     VGVALVDVIAGLNASTGILAALHDRKTMRQGQLVEINLLSTALAALANQSSAFLNTGA
                     SPSRLGNVHPSIEPFSTYATATRPIMICAGNDRQFSQLAVTIGAPGLTDD"
     gene            821..>1512
                     /locus_tag="PLJ64_13085"
     CDS             821..>1512
                     /locus_tag="PLJ64_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC94465.1"
                     /translation="MDQTATVTPGTDPAAAPPALSIRGLTKRYADGTLALEDLDLEIP
                     DGSFFGLLGPNGAGKTTLIGAVCNLIRVTGGEISVFGRPGTGMAARRMIGIAEQEPNL
                     DKFLTVYETLLYHGGYYGMDGREASERAEQMIDAFDLRAKRDVEAPKLSGGMRRRLLL
                     ARALLHRPRLVILDEPTAGVDLELRHELWRYIRGLHEQGTTILLTTHYLEEAEALCDE
                     IALISGGHIVAR"
BASE COUNT          249 a          501 c          522 g          240 t
ORIGIN      
        1 atcatcggtc aggccggggg cgccgatcgt caccgcgagc tggctgaact gccggtcgtt
       61 tccggcgcag atcatgatcg gccgggtggc cgtggcatag gtcgagaacg gttcgatgct
      121 ggggtggacg ttgcccagcc ttgagggcga ggcaccggtg ttgagaaagg cggagctctg
      181 gttcgcgagg gcggcgaggg cggtcgaaag aaggttgatc tcgaccagct gcccctggcg
      241 catggtcttg cggtcatgaa gcgcggcgag gattccggtt gaggcattga ggccggcgat
      301 cacgtcaacc agggcgaccc ccaccttgct tgcctcaccg tcggggggtc cggtgatcga
      361 catcattccg ctgagagcct ggatcagcgg gtcgtaaccc ggcatggccg cccccggtcc
      421 cgagccgaac ccggtgatcg agcagtagac gagcgatgga tgggcggcat ggacggtttc
      481 ccagtcgagg ccgaagcggg ccatggtgcc gggccggaag ttctcgatca ggacatcggc
      541 ccgcatcgcc agctcgaagg ccagggcgcg gtcttcctcc tgcttcagat cgagggtgat
      601 cgagcgcttg ttgcgattcg cggtctggaa gtaggtggag cggccggccg cgtcaaccgg
      661 gggtacccag ccccttgttt cgtcgccacc cggcggctcg accttgatca cgttggcgcc
      721 gagatcgccg agggtcatgg cggccagcgg cccggccaga acccggctga agtcggccac
      781 gatgacgccc gagagtgcgc tcatggttgc atggtgacgc atggaccaga ccgcgacagt
      841 cacccccggc accgatccgg ccgccgcccc tcccgcactt tcgatccgcg ggctgaccaa
      901 gcgctacgcg gatggcaccc tcgccctcga agacctcgac ctcgagatcc ccgacggcag
      961 cttcttcggg ctgctcgggc cgaacggagc cggcaagacg acactgatcg gagcggtctg
     1021 caacctgatc cgcgtgaccg gcggcgagat ctcggttttc ggccggcccg gcaccggcat
     1081 ggccgcccgc cggatgatcg gcatcgccga gcaggagccg aacctcgaca agttcctcac
     1141 cgtttacgag accctgctct accacggcgg ctactacggc atggacggtc gcgaggcgag
     1201 tgaacgggcc gagcagatga tcgacgcctt cgacctgcga gccaaacggg acgtggaagc
     1261 gccgaagctc tccggtggca tgcgtcgccg cctgctgctg gcccgggccc tgcttcaccg
     1321 gccccggctg gtcatcctcg acgagccgac cgccggcgtg gacctggaat tgcggcatga
     1381 gctctggcgc tacatccggg gtctccacga gcagggcacc acgatcctcc tgaccaccca
     1441 ctacctggag gaagccgagg cgctctgcga cgagatcgcc ctgatcagcg gcggtcacat
     1501 cgtcgcccgg ga
//
